LOCUS       DAZUMV010000001        92248 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000001 DAZUMV010000000
VERSION     DAZUMV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.001"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..513
                     /locus_tag="ACG8FK_000001"
     CDS             <1..513
                     /locus_tag="ACG8FK_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5095647.1"
                     /translation="DYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEMSP
                     YYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTY
                     IEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELK
                     KKRRRTQKFL"
     gene            641..1324
                     /locus_tag="ACG8FK_000002"
     CDS             641..1324
                     /locus_tag="ACG8FK_000002"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS5095648.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1633..1706
                     /locus_tag="ACG8FK_000003"
     tRNA            1633..1706
                     /locus_tag="ACG8FK_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1667..1669,aa:Arg,seq:tct)
     gene            1865..2224
                     /locus_tag="ACG8FK_000004"
     CDS             1865..2224
                     /locus_tag="ACG8FK_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS5095649.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2347..3978
                     /locus_tag="ACG8FK_000005"
     CDS             2347..3978
                     /locus_tag="ACG8FK_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS5095650.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4040..4681)
                     /locus_tag="ACG8FK_000006"
     CDS             complement(4040..4681)
                     /locus_tag="ACG8FK_000006"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5095651.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4867..4943
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4963..5271
                     /gene="rplU"
                     /locus_tag="ACG8FK_000007"
     CDS             4963..5271
                     /gene="rplU"
                     /locus_tag="ACG8FK_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS5095652.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5298..5642
                     /locus_tag="ACG8FK_000008"
     CDS             5298..5642
                     /locus_tag="ACG8FK_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS5095653.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5655..5948
                     /gene="rpmA"
                     /locus_tag="ACG8FK_000009"
     CDS             5655..5948
                     /gene="rpmA"
                     /locus_tag="ACG8FK_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS5095654.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6382..9189
                     /locus_tag="ACG8FK_000010"
     CDS             6382..9189
                     /locus_tag="ACG8FK_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5095655.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9380..9871
                     /locus_tag="ACG8FK_000011"
     CDS             9380..9871
                     /locus_tag="ACG8FK_000011"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5095656.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9974..10963
                     /locus_tag="ACG8FK_000012"
     CDS             9974..10963
                     /locus_tag="ACG8FK_000012"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5095657.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10996..11799
                     /locus_tag="ACG8FK_000013"
     CDS             10996..11799
                     /locus_tag="ACG8FK_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS5095658.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11820..12734
                     /locus_tag="ACG8FK_000014"
     CDS             11820..12734
                     /locus_tag="ACG8FK_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5095659.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12817..13191
                     /locus_tag="ACG8FK_000015"
     CDS             12817..13191
                     /locus_tag="ACG8FK_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS5095660.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13330..13575
                     /locus_tag="ACG8FK_000016"
     CDS             13330..13575
                     /locus_tag="ACG8FK_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5095661.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13747..14811
                     /locus_tag="ACG8FK_000017"
     CDS             13747..14811
                     /locus_tag="ACG8FK_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS5095662.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15057..15482
                     /locus_tag="ACG8FK_000018"
     CDS             15057..15482
                     /locus_tag="ACG8FK_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5095663.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15475..15924
                     /gene="nusB"
                     /locus_tag="ACG8FK_000019"
     CDS             15475..15924
                     /gene="nusB"
                     /locus_tag="ACG8FK_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS5095664.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16021..16875
                     /locus_tag="ACG8FK_000020"
     CDS             16021..16875
                     /locus_tag="ACG8FK_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS5095665.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16876..18213
                     /gene="xseA"
                     /locus_tag="ACG8FK_000021"
     CDS             16876..18213
                     /gene="xseA"
                     /locus_tag="ACG8FK_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS5095666.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18218..18442
                     /locus_tag="ACG8FK_000022"
     CDS             18218..18442
                     /locus_tag="ACG8FK_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS5095667.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18444..19334
                     /locus_tag="ACG8FK_000023"
     CDS             18444..19334
                     /locus_tag="ACG8FK_000023"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5095668.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19343..20161
                     /locus_tag="ACG8FK_000024"
     CDS             19343..20161
                     /locus_tag="ACG8FK_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS5095669.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20268..20717
                     /locus_tag="ACG8FK_000025"
     CDS             20268..20717
                     /locus_tag="ACG8FK_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5095670.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20735..22417
                     /gene="recN"
                     /locus_tag="ACG8FK_000026"
     CDS             20735..22417
                     /gene="recN"
                     /locus_tag="ACG8FK_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS5095671.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22472..22588)
                     /locus_tag="ACG8FK_000027"
     CDS             complement(22472..22588)
                     /locus_tag="ACG8FK_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS5095672.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22661..23617)
                     /locus_tag="ACG8FK_000028"
     CDS             complement(22661..23617)
                     /locus_tag="ACG8FK_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5095673.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23788..24156
                     /locus_tag="ACG8FK_000029"
     CDS             23788..24156
                     /locus_tag="ACG8FK_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS5095674.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24326..24757
                     /gene="mraZ"
                     /locus_tag="ACG8FK_000030"
     CDS             24326..24757
                     /gene="mraZ"
                     /locus_tag="ACG8FK_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS5095675.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24778..25737
                     /gene="rsmH"
                     /locus_tag="ACG8FK_000031"
     CDS             24778..25737
                     /gene="rsmH"
                     /locus_tag="ACG8FK_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS5095676.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25742..26143
                     /gene="ftsL"
                     /locus_tag="ACG8FK_000032"
     CDS             25742..26143
                     /gene="ftsL"
                     /locus_tag="ACG8FK_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS5095677.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26144..28336
                     /locus_tag="ACG8FK_000033"
     CDS             26144..28336
                     /locus_tag="ACG8FK_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5095678.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28362..29324
                     /gene="mraY"
                     /locus_tag="ACG8FK_000034"
     CDS             28362..29324
                     /gene="mraY"
                     /locus_tag="ACG8FK_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS5095679.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29338..30699
                     /gene="murD"
                     /locus_tag="ACG8FK_000035"
     CDS             29338..30699
                     /gene="murD"
                     /locus_tag="ACG8FK_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS5095680.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYRHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30725..31813
                     /gene="murG"
                     /locus_tag="ACG8FK_000036"
     CDS             30725..31813
                     /gene="murG"
                     /locus_tag="ACG8FK_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS5095681.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31828..33048
                     /locus_tag="ACG8FK_000037"
     CDS             31828..33048
                     /locus_tag="ACG8FK_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS5095682.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33204..34529
                     /gene="ftsA"
                     /locus_tag="ACG8FK_000038"
     CDS             33204..34529
                     /gene="ftsA"
                     /locus_tag="ACG8FK_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS5095683.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34552..35793
                     /gene="ftsZ"
                     /locus_tag="ACG8FK_000039"
     CDS             34552..35793
                     /gene="ftsZ"
                     /locus_tag="ACG8FK_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS5095684.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35810..36487
                     /locus_tag="ACG8FK_000040"
     CDS             35810..36487
                     /locus_tag="ACG8FK_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS5095685.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36501..37100
                     /locus_tag="ACG8FK_000041"
     CDS             36501..37100
                     /locus_tag="ACG8FK_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS5095686.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37181..37402
                     /locus_tag="ACG8FK_000042"
     CDS             37181..37402
                     /locus_tag="ACG8FK_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS5095687.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37451..38233
                     /locus_tag="ACG8FK_000043"
     CDS             37451..38233
                     /locus_tag="ACG8FK_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS5095688.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38254..38961
                     /locus_tag="ACG8FK_000044"
     CDS             38254..38961
                     /locus_tag="ACG8FK_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS5095689.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39223..42021
                     /gene="ileS"
                     /locus_tag="ACG8FK_000045"
     CDS             39223..42021
                     /gene="ileS"
                     /locus_tag="ACG8FK_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS5095690.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42070..43596)
                     /gene="zwf"
                     /locus_tag="ACG8FK_000046"
     CDS             complement(42070..43596)
                     /gene="zwf"
                     /locus_tag="ACG8FK_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS5095691.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43611..44258)
                     /locus_tag="ACG8FK_000047"
     CDS             complement(43611..44258)
                     /locus_tag="ACG8FK_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5095692.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44442..44771)
                     /locus_tag="ACG8FK_000048"
     CDS             complement(44442..44771)
                     /locus_tag="ACG8FK_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS5095693.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44948..45676
                     /gene="sfsA"
                     /locus_tag="ACG8FK_000049"
     CDS             44948..45676
                     /gene="sfsA"
                     /locus_tag="ACG8FK_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS5095694.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45692..46705
                     /locus_tag="ACG8FK_000050"
     CDS             45692..46705
                     /locus_tag="ACG8FK_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5095695.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46705..47982
                     /locus_tag="ACG8FK_000051"
     CDS             46705..47982
                     /locus_tag="ACG8FK_000051"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5095696.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48045..50747
                     /locus_tag="ACG8FK_000052"
     CDS             48045..50747
                     /locus_tag="ACG8FK_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5095697.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50899..51216
                     /locus_tag="ACG8FK_000053"
     CDS             50899..51216
                     /locus_tag="ACG8FK_000053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5095698.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51246..51566
                     /locus_tag="ACG8FK_000054"
     CDS             51246..51566
                     /locus_tag="ACG8FK_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5095699.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51674..53134
                     /locus_tag="ACG8FK_000055"
     CDS             51674..53134
                     /locus_tag="ACG8FK_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5095700.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53202..53423
                     /locus_tag="ACG8FK_000056"
     CDS             53202..53423
                     /locus_tag="ACG8FK_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095701.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53454..53636
                     /locus_tag="ACG8FK_000057"
     CDS             53454..53636
                     /locus_tag="ACG8FK_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS5095702.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53636..54049
                     /locus_tag="ACG8FK_000058"
     CDS             53636..54049
                     /locus_tag="ACG8FK_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS5095703.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54172..55353
                     /locus_tag="ACG8FK_000059"
     CDS             54172..55353
                     /locus_tag="ACG8FK_000059"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5095704.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55424..55588)
                     /locus_tag="ACG8FK_000060"
     CDS             complement(55424..55588)
                     /locus_tag="ACG8FK_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095705.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55742..55815
                     /locus_tag="ACG8FK_000061"
     tRNA            55742..55815
                     /locus_tag="ACG8FK_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55776..55778,aa:Arg,seq:cct)
     gene            complement(55884..57023)
                     /locus_tag="ACG8FK_000062"
     CDS             complement(55884..57023)
                     /locus_tag="ACG8FK_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5095706.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            57195..57329
                     /locus_tag="ACG8FK_000063"
     CDS             57195..57329
                     /locus_tag="ACG8FK_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095707.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(57322..57957)
                     /locus_tag="ACG8FK_000064"
     CDS             complement(57322..57957)
                     /locus_tag="ACG8FK_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095708.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58070..58705)
                     /locus_tag="ACG8FK_000065"
     CDS             complement(58070..58705)
                     /locus_tag="ACG8FK_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFS5095709.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58739..59200)
                     /locus_tag="ACG8FK_000066"
     CDS             complement(58739..59200)
                     /locus_tag="ACG8FK_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFS5095710.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59330..59761)
                     /locus_tag="ACG8FK_000067"
     CDS             complement(59330..59761)
                     /locus_tag="ACG8FK_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5095711.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59779..60099)
                     /locus_tag="ACG8FK_000068"
     CDS             complement(59779..60099)
                     /locus_tag="ACG8FK_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5095712.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60398..61174
                     /locus_tag="ACG8FK_000069"
     CDS             60398..61174
                     /locus_tag="ACG8FK_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS5095713.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61189..61392
                     /locus_tag="ACG8FK_000070"
     CDS             61189..61392
                     /locus_tag="ACG8FK_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5095714.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61408..61746
                     /locus_tag="ACG8FK_000071"
     CDS             61408..61746
                     /locus_tag="ACG8FK_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS5095715.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADNTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61733..61912
                     /locus_tag="ACG8FK_000072"
     CDS             61733..61912
                     /locus_tag="ACG8FK_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095716.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(61955..62425)
                     /locus_tag="ACG8FK_000073"
     CDS             complement(61955..62425)
                     /locus_tag="ACG8FK_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095717.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62512..63210
                     /locus_tag="ACG8FK_000074"
     CDS             62512..63210
                     /locus_tag="ACG8FK_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS5095718.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63388..63729
                     /locus_tag="ACG8FK_000075"
     CDS             63388..63729
                     /locus_tag="ACG8FK_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095719.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63722..64393
                     /locus_tag="ACG8FK_000076"
     CDS             63722..64393
                     /locus_tag="ACG8FK_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5095720.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64399..65085
                     /locus_tag="ACG8FK_000077"
     CDS             64399..65085
                     /locus_tag="ACG8FK_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5095721.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            65088..65837
                     /locus_tag="ACG8FK_000078"
     CDS             65088..65837
                     /locus_tag="ACG8FK_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5095722.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            65849..66118
                     /locus_tag="ACG8FK_000079"
     CDS             65849..66118
                     /locus_tag="ACG8FK_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095723.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            66123..66287
                     /locus_tag="ACG8FK_000080"
     CDS             66123..66287
                     /locus_tag="ACG8FK_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095724.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            66280..66582
                     /locus_tag="ACG8FK_000081"
     CDS             66280..66582
                     /locus_tag="ACG8FK_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095725.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            66579..66740
                     /locus_tag="ACG8FK_000082"
     CDS             66579..66740
                     /locus_tag="ACG8FK_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5095726.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            66737..67042
                     /locus_tag="ACG8FK_000083"
     CDS             66737..67042
                     /locus_tag="ACG8FK_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095727.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            67042..67398
                     /locus_tag="ACG8FK_000084"
     CDS             67042..67398
                     /locus_tag="ACG8FK_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS5095728.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            67358..67603
                     /locus_tag="ACG8FK_000085"
     CDS             67358..67603
                     /locus_tag="ACG8FK_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095729.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            67600..68019
                     /locus_tag="ACG8FK_000086"
     CDS             67600..68019
                     /locus_tag="ACG8FK_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS5095730.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            68016..68573
                     /locus_tag="ACG8FK_000087"
     CDS             68016..68573
                     /locus_tag="ACG8FK_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS5095731.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            68570..68866
                     /locus_tag="ACG8FK_000088"
     CDS             68570..68866
                     /locus_tag="ACG8FK_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095732.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            68943..69356
                     /locus_tag="ACG8FK_000089"
     CDS             68943..69356
                     /locus_tag="ACG8FK_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5095733.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            69665..69817
                     /locus_tag="ACG8FK_000090"
     CDS             69665..69817
                     /locus_tag="ACG8FK_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095734.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(69814..70089)
                     /locus_tag="ACG8FK_000091"
     CDS             complement(69814..70089)
                     /locus_tag="ACG8FK_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095735.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            70585..70749
                     /locus_tag="ACG8FK_000092"
     CDS             70585..70749
                     /locus_tag="ACG8FK_000092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095736.1"
                     /translation="MPATLNAYDGYDYRKKIVKKLYGATYGNVVSVLSVHHQASVTVA
                     EVEDAAVNGE"
     gene            70945..71112
                     /locus_tag="ACG8FK_000093"
     CDS             70945..71112
                     /locus_tag="ACG8FK_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS5095737.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            71138..71482
                     /locus_tag="ACG8FK_000094"
     CDS             71138..71482
                     /locus_tag="ACG8FK_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS5095738.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            71487..71768
                     /locus_tag="ACG8FK_000095"
     CDS             71487..71768
                     /locus_tag="ACG8FK_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095739.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            71871..72185
                     /locus_tag="ACG8FK_000096"
     CDS             71871..72185
                     /locus_tag="ACG8FK_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS5095740.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            72163..73857
                     /locus_tag="ACG8FK_000097"
     CDS             72163..73857
                     /locus_tag="ACG8FK_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5095741.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            73877..75055
                     /locus_tag="ACG8FK_000098"
     CDS             73877..75055
                     /locus_tag="ACG8FK_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5095742.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            75018..75704
                     /locus_tag="ACG8FK_000099"
     CDS             75018..75704
                     /locus_tag="ACG8FK_000099"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS5095743.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            75704..76864
                     /locus_tag="ACG8FK_000100"
     CDS             75704..76864
                     /locus_tag="ACG8FK_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5095744.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            76874..77749
                     /locus_tag="ACG8FK_000101"
     CDS             76874..77749
                     /locus_tag="ACG8FK_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095745.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            77746..78057
                     /locus_tag="ACG8FK_000102"
     CDS             77746..78057
                     /locus_tag="ACG8FK_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095746.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            78047..78400
                     /locus_tag="ACG8FK_000103"
     CDS             78047..78400
                     /locus_tag="ACG8FK_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5095747.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            78390..78791
                     /locus_tag="ACG8FK_000104"
     CDS             78390..78791
                     /locus_tag="ACG8FK_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095748.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            78784..79188
                     /locus_tag="ACG8FK_000105"
     CDS             78784..79188
                     /locus_tag="ACG8FK_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095749.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            79200..79808
                     /locus_tag="ACG8FK_000106"
     CDS             79200..79808
                     /locus_tag="ACG8FK_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5095750.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            79828..80190
                     /locus_tag="ACG8FK_000107"
     CDS             79828..80190
                     /locus_tag="ACG8FK_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095751.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            80193..80375
                     /locus_tag="ACG8FK_000108"
     CDS             80193..80375
                     /locus_tag="ACG8FK_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095752.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            80392..83823
                     /locus_tag="ACG8FK_000109"
                     /pseudo
     CDS             80392..83823
                     /locus_tag="ACG8FK_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            83874..84611
                     /locus_tag="ACG8FK_000110"
     CDS             83874..84611
                     /locus_tag="ACG8FK_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5095753.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            84621..86912
                     /locus_tag="ACG8FK_000111"
     CDS             84621..86912
                     /locus_tag="ACG8FK_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS5095754.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            86936..89062
                     /locus_tag="ACG8FK_000112"
     CDS             86936..89062
                     /locus_tag="ACG8FK_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095755.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            89079..89228
                     /locus_tag="ACG8FK_000113"
     CDS             89079..89228
                     /locus_tag="ACG8FK_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095756.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            89225..89671
                     /locus_tag="ACG8FK_000114"
     CDS             89225..89671
                     /locus_tag="ACG8FK_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095757.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            89673..89810
                     /locus_tag="ACG8FK_000115"
     CDS             89673..89810
                     /locus_tag="ACG8FK_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS5095758.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            89848..90141
                     /locus_tag="ACG8FK_000116"
     CDS             89848..90141
                     /locus_tag="ACG8FK_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095759.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            90138..90362
                     /locus_tag="ACG8FK_000117"
     CDS             90138..90362
                     /locus_tag="ACG8FK_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS5095760.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            90359..91384
                     /locus_tag="ACG8FK_000118"
     CDS             90359..91384
                     /locus_tag="ACG8FK_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS5095761.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            91574..91647
                     /locus_tag="ACG8FK_000119"
     tRNA            91574..91647
                     /locus_tag="ACG8FK_000119"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:91608..91610,aa:Ile,seq:cat)
BASE COUNT        31176 a        15052 c        19380 g        26640 t
ORIGIN      
        1 gattacaaaa aaataacttt tataagttct ataacgttga ttatattttt aattcttatt
       61 cttctcgtcg tcttggttcc ccgaattttt ggtttgactc cttatattat tgaggacaaa
      121 gaaatgagtc catattatgc acaaggaaat ctgatatatg tacgaccgaa aaatacaaat
      181 gagattttag tcggagatat tattacatat tatgaaaatt caggaaaaaa aatctcgacg
      241 cgtcgcgtaa tagccgttga aaacaaccac gaagattttt atacaaaagc tgatacaaag
      301 acttatattg agcctggagt agtaaaaaag agaaatatta tcggtagtcc catctttcag
      361 attccatata ttggactagt cctttcaaaa acggcatttg catggattca ggggttattc
      421 tttacgattg ctttttgcct aacaggaatc acagcatgga atacctttgt agagttgaaa
      481 aagaagcgga gacgaacaca gaaatttttg taagaatgtt aaaaacataa atgtattcca
      541 gagatccaac gactcttctg aacatgaaac acgagaaaac actcatgcag cttgtagatt
      601 tagtcagttg ttcgtataat gtgtgaagag gaggagtagt atgcgcaatg aaatgatgca
      661 tcgtcctgta gtcaaagaag aactattgga ttttatgcgt acccagcaaa aaaaattacc
      721 gggtgagcta ggcaaactgg aagaagaagc tcatgtagca gaagtaccag ttattcccca
      781 tgaaacagtt gtatttttga aatttttact tggacagctt cagcctgaac gaatcttgga
      841 gatcggagca gctatcggtt tttcatcaag tgtcatggcc acgacaatcg gagaaaacgc
      901 tcatgttacg acaattgatc gtttcgatgt catgatcgag aaagctaaaa aaaactatga
      961 acgtctagga ctgacagata aagtgacttt gcttgaaggt caagcagcag atatcttgcc
     1021 ggagttgtct ggtccgtatg actttatttt tatggatagt gcaaaatcaa aatatattga
     1081 atttttacct gaatgcttac gtttgttaag aaaaggtggc gttttgatgg tagatgacat
     1141 cttccaagga ggaacgatct tgctcccaga tgaagagatt ccccgtggca aacgagctat
     1201 ccatcgtaaa ttaaatgaat ttcttcgtgt tgtgatggat catcccgatt tgacatcgac
     1261 aatcttgcca ttaggtgatg gtgtgatctt aatgacaaag gaatcagaaa caatcgagtt
     1321 gtgatacatg attatggcag cctgtagata atcgattgaa tcgagggctc aattcgaact
     1381 tgatgtaaaa gtttctatat ttgtaagtca attaaaggat acaggaaatc taaataaaca
     1441 ggagtagcat gaagtcaatg tagctcctgt tttcaattga taaccttaga acagctgaag
     1501 gagaatgtct cgttttaaaa gattgattta gtagttgggc caaaataatt cattatttgc
     1561 taattaaata actatttcgt attgacagaa gcaatttaat tatctataat tattgatgtt
     1621 ccgctgattc ttgtcatagt agctcagctg gatagagcat ccgccttcta agcggacggt
     1681 cgggggttcg aatccctcct gtgacgctaa aaaaccgtta gaaactgagg tttctaacgg
     1741 tttttctttg ttttttgtgc caatattgtt tgttttcaaa ggcaaataaa ataaaaaaac
     1801 atccttatcg agtacaatta taaggagcaa aaaaataaaa ctcatcagaa aagaggaaga
     1861 agatatgacg atactatcta gaattttagt gactctagtt gcaattgaat ttttttatat
     1921 catgtatatc gaaacggtga gaactgattc tgacacgaca agtagggttt ttaaaatgag
     1981 taaagaagag ttaagcagaa aatctgttca aacattgttt aagaaccaag gagtgtacaa
     2041 tggactatta ggagtgggac tactttatgg agcttatctt tcttccgcat ctaaagaaat
     2101 cacaagtatg ctattaatat ctattttttt tgttgcatta tatggcagtt tgactagcga
     2161 tcgttggatt ttactcaaac aaggtgggct agctctgttg gcattgcttt ctattttctt
     2221 ataaatatag aaaaaataag aatttatcta acaggaaact ttgtgaaaaa tattacttta
     2281 tctcatgaaa tatgatattc taatgaatgg tattttagaa aatttcatga aaaaggagtt
     2341 agataggtgt tcaaaaatta tgtttcctca ctaaaaaaag aattttcagg ttatgatctt
     2401 tcaagatttc agaaagatct tttggctggg atcacagtag ctgctgtagc attgccttta
     2461 gccttggctt ttggggtatc aagcggagct gatgcggccg caggtttgat tacggctatc
     2521 attgccggta tcgttattgg cggtttatca ggcggttttt atcaaatatc aggtccgaca
     2581 ggtgcgatgg cagctatctt gatgtctatt gcggcaacaa agggaatgca aggtgtattg
     2641 cttgctactt ttttagcggg tctattttta cttgcagcag gacttttccg attaggaaca
     2701 ctgacttctt ttatcccagc acccgtaatt acggggttta cttcaggtat tgctatcatt
     2761 attgcattag gtcagatcga taatttattt ggtgttcact cggaaggtac aaacgttatt
     2821 gaaaaactaa gtagttatca aacgatagga tttccgataa acacagcctc tttgatcatg
     2881 ggagggctag tcattttagg gatgtttatc tatccaaaaa aatgggcgaa acgaatgcct
     2941 agttcgctgc tggctatcat tttggcaaca gcagttatgc tattgttcca tttaccagta
     3001 gcaactgtgg gtaagattcc acaaacattg atcagtagta atcgtttgaa tatgggagat
     3061 tttagctttt ctgccttgca acaagtggca gttccagcta ttagcattgc tttattagga
     3121 atgattgaaa gcttgctatg tggtgcttcg gctggccgaa tggcaaataa gcccttagat
     3181 agcaatcaag aattaatggc gcaaggaatt ggaaatctgt tgcttccttt ctttggcggg
     3241 attcctgcta ctgcggccat cgcccgtaca agcgtggcca tcaaatctgg tgcacaaact
     3301 agattagctg gtatgataca tgcagttgtc ttactgctct ccatgcttgt ttttgcgcca
     3361 gtgatgtcac agattccaat gccagcactt gcaggtgttt tgattgtgac ggcttggcgt
     3421 atgaatgaat gggaaacgat caaggagcta tttactaata aatattggtc agcagttcta
     3481 ttgtttatcc ttacgatgat ttgtacagtt atctttgatt taagtattgc tatcgtgatc
     3541 ggggtgatcg gcggctgtgt attttttgtt gtcaaaagcg ctgctatcac gatttcatta
     3601 gaaacgatcg attggcaaag aatggggctg ccagaaacgg aaaagctgaa taactggaca
     3661 gtagtgtata ttagtggtcc actgtttttt atgtcttcag aaaaactcaa aaataaattg
     3721 gaagaactaa atcatcacga tggtgtcatt ttctccatgc gaggcgtacc aagtattgat
     3781 ttgacggcac gtgcagtttt tgatgaattt caagaaaaag cagagcaaag aggacaaacg
     3841 attatctata cttccttgca gccagaagta gaaaaacaac tttctcactt gtgggaacat
     3901 acattcacgc atcaacatca aacagttgct catgcgttga cagctttaca tgcgaaatat
     3961 actgcaatta gccattaaaa caatgaaaaa gaacagacaa gactgtaaac tgaagatttt
     4021 atggtcttgt ctgttctttt taatattgct gtaaaagtga agctagtact tgccagtctt
     4081 gatggataac aggtacaagg gagcgattgt agaaaatagc tgctggatga aaaagtggaa
     4141 gaattcgata gtttttttct gaccattcgt aggcatcctt ttgttctgtt aattgtaaga
     4201 caggccctgt atagagttga ccatgatttt tggtaaccgt atattctttg ccaagtaaac
     4261 gttgaagacc aatgtttcca agtgtcgcaa ttaattttgg ctggaccttc atcagttcgt
     4321 aatccaatat tggtgcatgc gcaaatactt cggaacgagt aggtgtccga tttggataaa
     4381 cagtttctga ctgttgtgtc cgtttattga tccgatgagt cacgcgatac gggcggctcc
     4441 ttacagcact ggtgatatac acctcttctc gtgttaagcc agttgaagaa agtgcctgca
     4501 tcaattcttt tccagcttga ccgctaaaag gaacatgcgt atcaatctct gttttccctg
     4561 gggcttctcc cacgagcatc agtttaggat gaagaggtcc ttgcccagcg ataaatcctt
     4621 ctacaggacg gccgttcatt cttaaagaag cttcttgtat cagttctttt gggtattcca
     4681 tttatgctcc tcctttttct tttattataa gaataaaaga aacacaatgc gactatttta
     4741 tttggtagaa ctgtgaggat agaaaagcag aagtattgct tgaatttttg ttttactttt
     4801 tgattttaaa tttagttgac taacggttgc tgttctggta agctagtact gttgtaattg
     4861 tggactccac agctacaacc gctcagaaca agtaataagc acagcatatg ctgtcacttc
     4921 ggatggcgag tctaagtata tcagaaggag gtgctttata gcatgtacgc aattattaaa
     4981 actggtggta aacaagtaaa agttgaagtg ggacaagcaa tctatgttga aaaacttgac
     5041 gttgaagcag gagaaaaagt tgtttttgac gaagttatct tagtaggcgg agaatctaca
     5101 aaagtaggcg ctccaactgt tgcaggtgca actgttgaag gtactgttga aaaacacggc
     5161 aaacaaaaga aagtcgttac tttcaaatac aaacctaaaa aacattcaca ccgtaaacaa
     5221 ggtcatcgtc aaccatatac aaaagtggtt atcgacgcga tcaacgctta atagcgtttg
     5281 agaaagaagg cctatatatg atcaaaggaa catttaagcg aaatgacgca ggtcagatcg
     5341 tttcattcac tttgaccggg catgctgatg ctggtcctta tggaagtgat atcgtttgtg
     5401 cgggtgtttc tgcgttagcg atcagcactg tcaacgggat tgcctcatta gccggttttg
     5461 agccgattgt tgaaatgaat gaagaagaag gcggttatct ttataccgaa gtcacttcag
     5521 gaatgacaca agaacaaaac aatattgctg agattctatt agaaaatctt ttattaggat
     5581 tacaatcgat agaagctgaa aactcagaat atatccaaat taaaacaatt aatgataaat
     5641 aggaggtgca gaccatgttg ttatctatga atctacaatt atttgcccac aaaaaaggtg
     5701 gcggttctac atctaacggc cgtgactcag aatcaaaacg tctaggtgct aaacgtgctg
     5761 atggacaaac agttacaggt ggatcaattc tttaccgtca acgcggtaca aaaatctatc
     5821 caggtgtaaa cgtgggtatc ggtggagacg acactttatt tgcaaaagtt gatggcgtag
     5881 tacgtttcga acgtaaaggc cgtaacaaaa aacaagtgtc tgtttatcca gtagctcaag
     5941 aagcttaatt gaatcttatg attaaagagg ttgtgacaag acttttgtca cagccttttt
     6001 tgtttataaa aataaaagtt gtagtggagg ctacgaagtt tactttgtct ttttacactt
     6061 aatatttttt tgccaatttg gaatcaggat gttttgataa ctatcgtttt caattgatgg
     6121 aagctatatt gtttgctatc agtgaaataa ttatatggta aacagtgttc tttttcttgt
     6181 ttccattata aaaatcgtat cgggattttt cgttctttta aattgaacat ttctatctat
     6241 ccgcgtgtta tcaacaaaac aaaatcaggt aaatagttga cgaaacagaa ataaatacat
     6301 agtattatgt tttgaagcct aagtacaata gtctatagaa aaggagcgat accattggca
     6361 aaacgcattg accgaattta tatgtatgtc aaagaaaaaa ccgctcattt aacaaaggca
     6421 gaatatgatc aaggagtaac aacacaggaa attgctgaag tgttagggat tcagcgaaca
     6481 aatagcagta aagatctgaa tcagctagtg cgcgaaggaa aactgataaa gacagacgga
     6541 cgaccagtac gttacatata tcaaacactc gttacgcacg taaagccagt tacaaaacat
     6601 gtagtcagtt acaaagaaac gccggtctct gaagaaaaaa atacactcca aatagatact
     6661 aaggatatct ttgcaaaaat cattggagct aacggcagca tgaaaaattc agtggaacag
     6721 gcaaaagctg cgattttgta tccgccaaga ggattaaatt gtttgattac aggacctacc
     6781 ggatcaggaa agacttattt tgcacacgcg atgtatcatt ttgcaaaagc gcatcatgta
     6841 atagatgagg aaacggaact gattgttttc aattgtgctg attatgccaa taaccctgag
     6901 cttttgatga gtcatttatt tgggtacgca aaaggtgctt ttacaggcgc agatgaagaa
     6961 aaaatgggga ttatcgatca agcggatggc ggtatgctgt ttttggatga gattcaccgg
     7021 ttgccgcctg aaggtcaaga aatgatcttt tactttatgg atcatggaac ttacagtcgt
     7081 cttggggaaa caacgaaatc ccatgaagca aatgtacgga tcgtaggtgc gaccacagaa
     7141 gacccaggtt cttctctatt agaaactttt gttcggcgta tcccaatcaa tatcaaactt
     7201 ccggcttttg aaaaacgtcc tgcaaatgag aaagtcgatt tagtaaaaat catgattgcc
     7261 cacgaagcaa accgcacgca gcgaaagatt tctttgacag aagatgtagc aaaagcattg
     7321 atcggcagtg tgacctatgg aaatatagga caattgaagt caaatatcca gttagtatgt
     7381 gcacgaggat ttctaaatca tatgcaatca cccgaaatct cgatcacaat cgatgatttg
     7441 acagaaggga ttcgtagcgg attgattcaa ttagcaagca accgcagtgc aatggcagag
     7501 ctttctaaaa tattggagcc aaagataact gtagcaccaa atgatacgat catgaaaatc
     7561 caatcggact cctatgagtt accttataat ctatatgata tcatcggaga taaagcagct
     7621 ttactgaagt cggatggttt ggatcaagaa gcaatcaatc atttcatttc tacagatatc
     7681 aacatccatc tgaagtcttt ctataaagac catggttttt cttttaatgc agacagcaaa
     7741 ttagcagaat ttgtagatcc gaaaatcata gaagtgacca atcagattta tgtaatggtc
     7801 aaaagatctt taccatatga gtttcagcaa aactttattt atgctatgag cttgcatatc
     7861 agttcttttt tgaaaaaaat caacttagga gaagaacggc atacgaatga taacatccgt
     7921 gagatggctt ttgattttcc agatgaatat gctgttgcga aagaagtgcg tagttatatc
     7981 gaaacgtatt ttcaagtgga gattccagaa agtgaagact attacttgac cgttctactc
     8041 gtctcgttac gggcaaatca agcaagcggc agaatcggtg tagtcgttgc agctcacgga
     8101 aacagcacag caagcagtat ggtccaagtt gtccaacagt tgttagatgc agagaacgta
     8161 cgagcagtag acatgccttt ggatatggat ccgaaaacag cattggaccg aattgaacgc
     8221 aatgtacaag aagtagatga aggaagcggg gtcatactct tagtagatat ggggtccctt
     8281 gcgtcgttta attcgcaaat tcaaagagac actggaattc cagtacgtac tgttgatatg
     8341 gtaacaactt ctctagtttt ggaaactgta cgaaaggtct cagtattagg gacagatcta
     8401 gatatgttgc atgaatcatt gaaaaatttt cgaggttacg cagaaatatc ttctgtagaa
     8461 tctgttcagg aagcaaaacc tattcataag aaaaaagctg ttttggctat ctgcgcatct
     8521 ggcgaaggta cagcacaaag aatcaaagaa ttgatcgaac gagcggtaaa caagcgtcaa
     8581 gaaacagaat tagaagttct agctttgtcg attgtggaag tgaaagaaga actgccagtt
     8641 atccaaaaag aatatcagat catagctact actgggatta ctgatttgaa tatccaagcc
     8701 ccatttattc ctttagaacg ttttattgat caaaatatcg aagtcatttt ggatcagttg
     8761 ctgatggaat cagaattgga ggaaactgaa ttcatctccc tagatgaaga gtcagctaaa
     8821 aatacgtgtg ttgagtttat ctctgataac ttcatcttta ttaatggtag taagttaatc
     8881 gatccaatgt ggcaattttc tacacaaatc tctcagacga cgggaatcgg cgatgaagaa
     8941 tatggattca aaatcaacct agtgatgcac acagcaggaa tgatcgaacg tattattcgg
     9001 aatgaaccat tgactgtgga agaaaacgaa ttgacaaata caacgaatga tcctctttat
     9061 agccaattgg cagcctcagt tgttctgttg gaagatcaga taaaagtaaa agtgccgata
     9121 gaagaaatgt attatctgct acgtctagtc cataatcagt tagataaaaa agaatacact
     9181 gtgccatgaa tacacaaaaa cgatacactt ttatagtgta tcgtttttgt gtattggtta
     9241 agaatccttt aaattcaatt agtgtatcgt aaaaaagttg gcacgctttt tgcttatagt
     9301 agtgtgtgct aggaggtggt tgttcacaca gaaatgatag gggtttctgt gaagtagtga
     9361 ggaagaagga gttttgcaga tggatattcg attggcacgt atagatgatc gtttaatcca
     9421 cgggcaagtc gcaacagttt ggtcgaagca aacaggtgtt gaacgtatca ttgtgatcag
     9481 cgatgtagtc gctcaagata aattgagaaa gtttctacta aaagaagctg caccgccggg
     9541 gatcacagca aatgtaataa ccgtgcagaa aatgattcaa atctatcata gtggattatt
     9601 atcacgagca aaagtgatgc ttttattcac caatccccaa gatgttgaag cggttgtccg
     9661 ctgcggtgta cagttgaagt cgttgaatat cggcggtatg agttttactg aaggcaaatc
     9721 aatgattacc aattttgttt cagtaaatca acaagatatt gattcctttc gttacttgaa
     9781 cagtcaagaa gtggagttag agattcgaaa agtacctgct gacaggaaaa tccttttgat
     9841 ggaactacta gcgaaaatca aaaaggtgta gcaagaatgg ttgttggaaa aggtcaaata
     9901 attctgttgt aatggttagc aattattaat atttttgaat ttgttacaac aaaagtaaat
     9961 aggaggtata aatatggtag ggattatcct agcaagtcat ggacaatttg ctgaaggaat
    10021 cttacaatcc ggttcaatga tctttggcga acaagaaaac gtcaaagccg tgattttgaa
    10081 accaagtgaa ggtccagatg acttgagagc caaattggaa gaagcggttg cttcttttga
    10141 taatcaagac gaagtgttat tcttagttga tttatggggc ggaacaccgt tcaatcaatc
    10201 aaacacatta ttcgaagagc acaaagataa atgggcaatc gtcagcggtt tgaatttacc
    10261 aatgttgatt gaagcctacg catcacgctt ttcaatggaa tctgctcatg aaattgcagc
    10321 acacatcatt gaaacagcaa aagacggtgt aaaagtaaaa ccggaagaat tagaaccggc
    10381 agaagcaccg aaagcagcag ttgaagacgc acaacctaaa ggagcacttc cagaaggaac
    10441 tgtggtcgga gacggaaaaa tcaaatatgt attagcacgt gttgactcac gtctgttaca
    10501 tggacaagtt gcgacagctt ggacaaaagc agtgcaacca aaccgtatta tcgttgtttc
    10561 agatgcagta tcaaaggatg atctgcgtaa gagactaatc gaacaagctg ctcctccagg
    10621 tgtaaaagca aatgttattc ctattagcaa aatgatcgaa gtagcaaaag accctcgttt
    10681 tggaaatacc aaagcattgc ttttatttga aaatcctgaa gacgtattga cagctgttga
    10741 aggtggcgta gacatcaaag aattaaatgt tgggtcaatg gcgcattcgg tcggtaaagt
    10801 cgttgtaagt aaagtattgt ctatgggaca agaagacgtt gaagcgtttg agaagcttga
    10861 acaaaaaggt gtgaaattcg atgtacgtaa agtacctaat gattcacgcg ataacatgga
    10921 tgatattttg aaaaaagcaa aagctgaatt agcaaaagcg taataaataa tattaaaatt
    10981 tataggaggc ttatcatgtc tattatttca ataattttag tcgtattagt tgcctttcta
    11041 gctggtatgg aaggtattct tgatgaattt caatttcatc aaccactagt ggcatgtaca
    11101 ttgatcggtt tagttaccgg taacctcgaa gctggtatcg tacttggcgg tacgcttcag
    11161 atgatcgctt taggttgggc aaatatcggt gccgctgtag caccagatgc tgcattagca
    11221 tcagtagcat cagcaattat tttagtttta ggtggacaag gtgttaaagg tgttccttct
    11281 gcgatcgcga ttgccgttcc tttggctgtt gcagggttgt tcttaacaat gatcgtacgt
    11341 acaatcgctg ttccaatcgt tcatttgatg gatgcggctg ctgaagaagg aaacatccgt
    11401 aaggttgaaa tgtggcacat cattgctgta tgtttacaag gggttcgtat cgcggtccca
    11461 gcagcagcgt tgctatttat cccagcacaa actgtgcaat cattccttga atcaatgcca
    11521 gcatggttaa cagatggtat ggctatcggt gggggaatgg tcgtagctgt aggttacgct
    11581 ttggtaatca acatgatggc tacaaaagaa gtatggccat tcttcgttat tggtttcgtt
    11641 gtagctgcaa tttctcaatt aacattgatt gctctaggtg ctttaggtgt tgcattagct
    11701 cttatttact tgaacctttc taaaatgggt ggttcatcaa ataatggagg cggaagcaac
    11761 tctggtgacc ctttaggcga cattttgaat gactattaat cttgaaggag gaaaataaaa
    11821 tggcagaaga aaaaatcaaa ttatcaaaaa aagatcgttt agctgttgcg tggcgctcta
    11881 ccttcattca aggttcatgg aactacgaac gtatgcaaaa cggtggctgg gcattttcta
    11941 tgattccagc aatcaaaaaa ttatacaaaa caaaagaaga acgttctgca gcattaaaac
    12001 gtcacttgga attctttaat acacacccat acattgcttc tccaatttta ggggttacat
    12061 tggctctgga agaagaacgt gcgaatggcg cgcctgttga tgatgtagcg attcaagggg
    12121 ttaaagtagg tatgatgggt cctttagctg gtgttggtga cccagtgttc tggtttacag
    12181 tacgtccaat gctaggtgca ctaggtgctt cattagctat gggaggaaat atccttggac
    12241 caatcatctt cttcttaggt tggaacatta ttcgttgggc attcatgtgg tatacacaag
    12301 aatttggcta caaagctggt tctaaaatta ctgatgacct ttctggcgga ttgttacaag
    12361 acgtaacaaa aggtgcttca attttaggga tgttcgtatt agctgccctt gtagaaagat
    12421 gggtatcaat caaattctta ccagttgtat caactgtaaa attagataaa ggtgcttaca
    12481 tcgaatggga taaattgcct tcaggtggtg aaggaatcaa gagtgcattt gaacaagtta
    12541 ataatggctt agccctttct cctgaaaagg taacaacatt acaagacaac ttagaccaat
    12601 tgattcctgg tttagctgca ttaggattaa cattgttatg tatgtggttg cttaagaaaa
    12661 aggtttctcc aatcgttatc atccttggtt tattcgtagt cggtgttgtt ggacacgtca
    12721 tcggactttt ataatccata aaaatatagc tcgggcgttg tcccgagctt tttttatata
    12781 gtatactgtt cataaacaaa tatctggagg gagaaaatgg ttcaatcaat caatacaaaa
    12841 gtagacttgg taattgatgc tacttcgttt acgggtctga cagattacgg gcaaatcatg
    12901 ataggtgata atggttttga gttttaccat gcgcgagata gacgcaaata tatccaaatt
    12961 ccatgggaag aagtggatta tgtactagca tcagtcatgc taaagggaaa atggattcct
    13021 cgatatgcca ttcaaacgaa aaaaaatggt acatacacat tttcttccaa agaacctaaa
    13081 aaagtgcttc gtgcgatcag agaatatgta gatgaagata gaatggtaca atcattgagt
    13141 ttctttgatg tagtgaaacg tggtattcgt agtatgttta aacgcaagta aaaaataaag
    13201 aaaacttcta agaggaggtt ttctttattt ttttactttg cagaagaaaa tatgaaatag
    13261 caaaaccaat tatttgtccc ctttaaatcc catatgctaa aattgattag caaagaagga
    13321 ggttatatta tgagttggtt atggacattg attgttggag ccgtgatcgg agcaattgca
    13381 ggtgcgatca ccagtaaagg aaattcgatg ggctgtgtaa cgaatatcat tgctggacta
    13441 gtcggttcaa gtattggaca agcgattttc ggcgaatggg gaccaaaagc tgcaggtatg
    13501 gcacttattc catctatttt aggcgcagtg attttggtag cagttgtctc atttttcttt
    13561 ggaaaacgat cataagtttt tacaaaatag taaactaaaa ggagttgcag cgagactgtg
    13621 actccttttt ggctttattt atttaaaata aaaagaagtt ttccggaaat ttttatttaa
    13681 ctgtcgtgta cgaagaaaag tttgctataa tggaaaaaag cgtgcaaaga aagagggagt
    13741 ttcattatga tgctacgtgt agaaaaattg cgcaaaaaaa tgcaagaaga aaatttggat
    13801 tcatttttgg ttaccagtcc gtacaatctt cgttacttaa cgaactttac aggtacgaca
    13861 ggattagctg ttattacttt ggaaaaagca ttttttatta ctgattttcg ttataccgag
    13921 caggcggcag cgcaggctca agggtttgaa attattaaaa atgtcggacc tatttttgat
    13981 gaggtagcaa acttagtacg caaagaagaa ctgcactcat tagcttttga ggaaacaaca
    14041 gtctcatttt tagaatattc ggttttagaa gaaatcatcg atgcggaatt agtacctgtt
    14101 tcacagatga tcgaagaact cagagaagtg aaagatgaag aagaaatcgc tattatcgaa
    14161 aaagcatgtc atattgcgga tatggcatat gatcacatcc tgaaaatgat ccaaccgggc
    14221 atgactgaga ttgaagtcgc aaatcaacta gacttttata tgcgttctct tggcgcttca
    14281 ggtgtttctt ttgatacgat cgttgctagc ggattgcgtt cggctatgcc gcatggtgta
    14341 gcaagcaaaa aaatcatcga acaaggtgac ttgatcacat tggactttgg ttgctattac
    14401 gaagggtacg tttccgatat gacacgtaca ttcgctattg gtgatcctgg ggaaaaatta
    14461 aaagaaattt atcaaatagt cttagatgct caattagcag ttattgatgc tgcaaaacca
    14521 ggaatgactg gtgtccaatt agatgcagtt gctagagatt atatttcaaa acatggttac
    14581 ggggaagcat ttggacattc tacaggacat gggatcggtt tagagatcca cgaaggacca
    14641 aacgtttctt ctcgtgcaga aaagcaattt gtaccaggaa atgtgatcac tgatgaacca
    14701 gggatctact taccaggaat cggtggagta cgtattgagg atgacttgtt gatcacagaa
    14761 gatggaaatc gtgtgttgac tcgttcacca aaagaattga tcatcttata gtttcatcaa
    14821 tagaagaagt aagcgtaaaa atcagtgttc acagactttg ttatagaaaa tcactcgatt
    14881 taatgataca ctatagaaga acttttaagc tgtaagagat aataaagttg ggtgttttct
    14941 ccaattcata gcagtttgca gctgatcggc ggcagttttc ttaccacgtg atcactatgc
    15001 cgcatcatag agaattcgga tgtatctcgt gcttaggcaa agggaggaat aaataaatgg
    15061 cagatgaaaa aaatttgatt ttaaacgcaa accaagattt aggagagatc gtgatcgctc
    15121 ctgaagtcat cgaagtaatt atcgggattg cagcttctaa agtagaaggc gtatacggaa
    15181 tgcgcggaac ttttgccagc aatgtcactg aattattagg acgtgcagct cacggaaaag
    15241 gtgtttactt agcaaacgag gaagatggct tgaaagtaga tctttactgt tatctagaat
    15301 atggtgtttc agttcctaaa gtagcaatgg aaatgcagga acgggtcagc cagcaagtcc
    15361 ttttcatgac ggatatcgat ttatctgaag tcaatatcca tgtagtagct gttgttcctg
    15421 aaaaattaga acaaccaaca atcgaagaac tttttgaaga cgaagaggaa gagaatgaat
    15481 aaagaattat caagacacga gattcgggaa atggcattgc aggctttatt tccattagac
    15541 ttcaatgcag acctaacaaa agaagatgcg attttcaacg caatcgaatt agaccaccgc
    15601 gacatgatca acgaagatga atcagagttt gttcctgttt atcttgatac gctagttggc
    15661 ggtgtctgtg caaaaaaaga tgaattggat aaagtgattg aaaaacattt aaaaaacaat
    15721 tggcacatca atcggatctc aaaaatggat ttggtgatct tgcgtatcgc gatttttgaa
    15781 atgacttatg tgacagatgt accagcggct gttgctttaa atgaagcaat cgagttggca
    15841 aaaacattta gtgatgaccg ttcacgtaaa ttcgtcaacg gtgttttatc caatgtattg
    15901 aaagaattag aagctggggc gtaagtccta gcttttttct atgaatcatc ataaacctgt
    15961 tataaatcgg gaaaattcta tggtagaatg aaaacacaaa aaatatctgg gagtggttag
    16021 atggcagaat tgattaacgg aaaagaatta gctgaaaaaa tgcaggcaga gattgctgaa
    16081 aaaatccaaa aattgaaaga taatggtatc catcccggat tagtcgtttt attggtaggg
    16141 gaaaatccag ccagccaaat ctatgtaaga aataaagaac gtgtagcaaa agaaatcgga
    16201 atccattcat tggtagagcg ttatcctgag acgatttctg aagacgaact acttgcagaa
    16261 attgaaaaat acaatcaaga tcctagtttt catggtatat tggtccaatt accattgcca
    16321 aagcatatag atgaagaaaa gatcctgtta gctatccgcc ctgaaaaaga cgtagacggt
    16381 tttcatccga tgaatttagg cagactcctt gcgggtaaac cggacaagat tccttgtact
    16441 ccatacggca tcatgaaaat gtttgaagct tattctatcg atccggcggg taaacgagca
    16501 gtagttatcg gacgaagcaa tatcgtcggt aaaccgatgg ctcagctact cttgatggca
    16561 gatgcaactg ttactgttgc tcattctaaa accgaaaatc tggctgaact ggcacaagaa
    16621 gcagatattt tagtcgtagc aataggacgt ggacattttg tcacaaaaga attcgtaaaa
    16681 ccaggtgctg tagtgataga tgtagggatg aatcgtgatg ctaacggtaa gctgatcggt
    16741 gatgtgaaat ttgatgaagt ggagcctatt gcaggctgga tcactcctgt gccaaagggt
    16801 gtagggccaa tgactatcac gatgctgatg tatcaaaccg taaaatctgc cgaagctttt
    16861 ggagcaggtg aatagatggc acaagaatat ttaactgtca ctacattaac gaagtatttg
    16921 aaaagaaaat ttgatgccga tccttattta gaacgcgtat atttaacagg cgaaatatcg
    16981 aatttccgtt tacgtgccaa tgcccatcaa tatttcagct taaaagatga ccatgcaaaa
    17041 atatctgcta ttatgttcaa aggagcattc caaaaattac gattccagcc aaaagaagga
    17101 atgaaagtcc ttgtcgttgg gcgtatttca ttatatgaag cgggaggttc ttatcaaatc
    17161 tatgtagagc acatggagcc tgatggaata ggtgctttat atcaagaatt ggaagaacga
    17221 aaagaaaagc tgatgaagga aggccttttt caagggccta aaaaaatgct gccacgtttc
    17281 ccaaagcgaa tcgcggtcct taccagtcca agcggagcgg ttatccgcga tatcatcacg
    17341 acggtgaaaa gaagatatcc aattgtccaa ttggttttgt tcccaacagt tgtacaagga
    17401 aaccaagcag cggacaatgt tgtcaggaat atccagagag tagaagagat gggcaatttc
    17461 gatacaatga tcatcggtcg tgggggtggt tcgattgagg atttatggcc gtttaatgaa
    17521 gagcgggttg ctcgggcgat ttatcaagct aatacgccgg tgatttcatc tgtaggacat
    17581 gaaacagata ccacgatcgc tgatttagta gcagatgttc gggctgccac tccaacagca
    17641 gctgcagaat tagctgttcc tgttttgtca gaagagttga tgcgcattca agaacggcaa
    17701 gctagattgc agcaggcgtt tgtacgccag atccagcgca aacaagaacg gttcgagcgt
    17761 gctcgacgtt cctatgtatt tcggcagcct gaaagattgt atgaaggtca gaccatcaaa
    17821 ttagaccagt tgaaacagcg attatatcaa gctacacagc aaatttatca tgcgaaagaa
    17881 aaacaaacga tcgcattaag ccaccaattg gaacaagtgg ctccgcttta tcgagttaaa
    17941 gcagctcgcc aagaaactgc ttatctagaa aaacgcttag aagagaaaat ccaacaatat
    18001 atgaaacaac agcagcagcg ttttgctcaa gctgttcaat ctcttgattt gttgagtcca
    18061 ttgaaaatca tgggaagagg ctacagctat acgacgttaa atgatcaagt agtaaaaagt
    18121 gtcaccgaga ttcatcaagg ggatttgctg acgatccatt atcaagatgg aatcgttaaa
    18181 ggacaagtac aagagataga caaaggagaa taaaaatatg gaaaatccaa cttttgaaga
    18241 gtcactgcaa gaattagaaa aaattgtcat gcagttagaa caaggagacg ttccattaga
    18301 atcagctcta gattcgttta aacgaggaat ggaattaagc aaacattgcc aagatacatt
    18361 agctaaagca gaagagacct tgacgaaaat gatgacagaa tcaaatgaag aagttgattt
    18421 tgacggaaac gaggaaagct gagatgagat taactgactt ttcagctatt catctacctg
    18481 ctgtagaaaa agaaatcttg tcttttttag atgagcatac aacggataga ggactctatg
    18541 atgcgatgag ttattcagtg aaagcaggcg ggaaaagaat acgtccctta ttacttttaa
    18601 cggccgttgc ttcctttgat gagcctattg atgttcccgt ctatcaagta gcagctgctt
    18661 tagaaatggt gcatacgtat tccttgatcc acgatgattt gcctgcgatg gacaatgatg
    18721 atcttcgtag aggaaaacct accaatcata aagtattcgg cgaagcatta gctatattgg
    18781 ccggagatgg tttgcttaca ggagcattcc agctaatcag tatggcacac ttaggtaatt
    18841 ctcctaagct tctgctgctt cagcaattag ctgtttgtgc gggatcacaa ggtatggttg
    18901 ctgggcaagc tgcagatatc gagggcgaat cgaaaaagct ttctttagaa gaattagcat
    18961 ttattcatga aagaaagaca gggcatttga ttcgttatgc cttgctggct ggtggcatat
    19021 tagcgaagca acctgaagaa atcttattgc tacttcaacg attagccgaa catttaggtt
    19081 tagcttttca gataagagat gatctactcg atgtgattgg tacaacgaaa acattaggga
    19141 aaacagctgg taaagatgaa cgtatggaaa aaaatactta cccgcgttta ttaggcctag
    19201 aaaagacacg tgaggcgttg gagattgagc tgttatccgc caacaagatc atagacaaat
    19261 tagaagagga cgttttttct tttgatggcg ggctgatgcg ccagatgatc aagcaatttg
    19321 atgtggagaa atgagggaaa gaatggaaaa agaacgcgta gatgtattag cggtcaaaca
    19381 aggattattt gaaacaagag aacaagccaa acgttcagtg atggctggtt tgatttacaa
    19441 cgaaaaaaac gagcggtttg ataaaccggg agaaaaaatt ccagtgagta gtgaattaag
    19501 agtcaaaggt aaaaaacttc cttatgtttc tcgaggggga ttaaaattag aaaaagcact
    19561 caaagcattt gatctatctg ttgaaggaaa aacattgtta gatattggtg catccactgg
    19621 cggatttact gatgcagctt tgcaaaatgg cgcaaaaatg agttatgctt tagacgttgg
    19681 ctacaatcag ctagcttgga aaataagaca agatcctaga gtcgtcgtaa tggaaagagt
    19741 gaattttcgt tatgcaaaac ccgaagactt taccttagga attccagaag ttgctgttat
    19801 cgatgtttct tttatttcac taaaactgat gctgcctcca ttacatgcta ttttaaaaga
    19861 agagggagaa gtgattgctt taatcaaacc ccaattcgaa gcaggcagag aagctgtagg
    19921 gaaaaacgga attgttcgtg accctcagac ccatcaaaaa gttttagaag acattttgtc
    19981 atttgcggca caaggtggat atgatgtgct ggaattaagt tattctccga tcacgggggg
    20041 agaaggaaat atcgagttct tggctcactt gaggaaagta cccgaaagcg gaacaatcaa
    20101 ttcagctatt aatatggcag aagtggtttc aaatgcacac gaacagtttg accataaata
    20161 aattgtaact gtgaaaatca agtgagaagt atgcggattc ttctttatta taccaaggaa
    20221 ataagctatc attgattaag aagaatatgc taaggagcga agacgtaatg agaaaaaaag
    20281 atcggcatcg tttgattact cgattgttga gtgagcaaga tatccgaaag caggaagaat
    20341 ttgttgaaat cctgaaaagc aaaggaatat ctgttactca agctacgatt tccagagata
    20401 taaaggaact gaaactgatc aaggtaccgg cttcagatgg cggctaccgt tatagcatac
    20461 cagctgaaac aaacgaagat gtaaatacta aattggaaaa actattaaaa gacgctttcg
    20521 tggcagttga ccaaatggaa aaatttgtta ttttgaaaac attgccggga aatgcttcgg
    20581 cagcagcaaa tttgattgat aaacgttaca agaaagaatt attttcgatt atcaatgatg
    20641 acgataatgt tttgatgatt acacgaactg aagaccaggc agttttattg aagaaagact
    20701 tcctgcatta cctttaagtt gaggagcgaa tgtgatgcta caagaaatca gtattactaa
    20761 ctttgcgatt attccggaat tgcggctttc ttttcacgaa ggaatgaccg cgctgaccgg
    20821 agagactggt gcgggtaaat ccattattat cgatgcttta ggtctgttag caggaggaag
    20881 aggttcaagc gattatatac gtcaaggagc agaaaaatgt gtgcttgaag ggttgtttga
    20941 actaccaaaa caagaaggat tctctgaact gatggttgaa ttagggatcg aaacagatga
    21001 ggacaatctg attgttcgac gggatatgtc cttaactgga aaaaatgttt gtcgagtgaa
    21061 tggtcatatt atcacattag ctaatctgag aagaatcggc agttatttag tagatatcca
    21121 aggacaaaac gaacatcagg aactgctcca gcctgaatca catcttgctt tattggatcg
    21181 ttttggagat gtagctttcc aacagaagaa aaagagttat cagcaagaat atatctctta
    21241 tcgtgagctg gaaaaaaggg tacgaaaagt gcagcaaaat gaaaaatcct atgttcagag
    21301 aatagatatg ctccatttcc agcaagaaga aatagctgct gctgatttgc aagtcggaga
    21361 agaagagaaa ttaaaagaag aaagagaaaa acttagcaat tatcaaaaaa ttgctgatgg
    21421 attagctgca ggttacggag cactgacgaa taatgaacaa aacagtgtag atggtgttgg
    21481 cttggccgtt tcagaactgc aagggatcgc ccatctagat gtggagtatg aagcaattta
    21541 tgaaaatatc caaagtgctt actatttgct gcaagatgca atcggcgata tgagtcgtca
    21601 gattgattta ttggagttag atgagagtcg tttagaagaa gttacgcaac gtttagaatt
    21661 aatccgacag ttgaaacgta aatacggcga atccattgag tcaatattgg cttactatga
    21721 cgaaatcacc gaagagctag cttcttctga tttttcagaa ggtcaattgg acaaaatgaa
    21781 agaggaacta gagcaaaaag agcttctact tcagcaacaa gccgcagatc ttcatgaagc
    21841 gcgtaaaaaa atcgcaaaag aattggaaaa atccattctg catgaattga aaagtctgta
    21901 tatggaaaac actgagtttg aggtacgttt cttaaaagaa gaaaatagac aattgaatca
    21961 tgatggattt gaccaaatcg aattttacat tacgaccaat ccaggagagc cactgaagcc
    22021 tttggtgaaa gtcgcttctg gtggcgaact gtctcggatg ttgcttgctt tgaaaactat
    22081 tttttcttct gagcaaggtg tgacaagtat tatttttgat gaagtggata caggtgtgag
    22141 cggaagagtt gcacaggcca tcgcagataa aatttctcaa atatcgaaat actctcaagt
    22201 tttgtgcatc actcatctgc ctcaagttgc tgcagtcgcc gactatcaat attatattgt
    22261 gaaagcagtg attgatggac gtacgcaaac ttctgtttca gagttgaaaa caaaagaaag
    22321 agaagaagaa attgcacgta tgctggctgg aagtgagatt acaaaactca cactcgaaca
    22381 tgcaaaagaa ctattgaaac tcgcaaaaag gacctgagcc acctcgcagt ggttcaggtc
    22441 cttttcagca gctgatagct atattttatt tttacatgac actcattaca gcagtcaata
    22501 caacagatcc aatcgttaaa atcagcattg tccaaatgac aaattttgtt actttagcaa
    22561 aagtacttgt ttgttttttt tcacgcattg ttttcaacct cgtcattaat tagttgattc
    22621 aagtgtacaa tcttttttcc atttccacaa gagggctttt ttataaaaac ttcccttttt
    22681 ttaaataata ggtagttacg atacaaataa ataatgttaa tagaactagc catataaagg
    22741 catcatccct ttcagcaata ggtaacttaa tgttcattcc atagatactg ccgacgattg
    22801 tcggtatagt taaaataatc gttaaagaag ttaaaatttt cataatatta ttcagattat
    22861 tcgatacgat tgctgaaaaa gtctgattca tttgttctac tagttttaat tgaatgcgag
    22921 aagtagtcat tgcctgctct gtttccacca atacatcacg taaatgattt ttcaaagcat
    22981 gattttcttg gaaaagttta ttttgcgcta gttctttcaa tgcatctaaa ttagcagaag
    23041 tcgcttctct gaaataaacg aggctttttt ggatatccat gatttgataa agttgtttgt
    23101 tttcagtgga tacttgaagt ttggcttcca aagtatcgca ttgagattga agcgtagcta
    23161 aataactgtt gtaagagagt gtgacctgcc aaatgaattg aaagaagata ttgagattag
    23221 gcgaaatatc actttttgga aaggtctggc taaagacttt ctttaaaaac agtggttcat
    23281 gattcgtaat tgtgataagc ttctttttgg gcgtcaaaat aatagagagc gggtatgtat
    23341 ccacttgaaa ataaccgcta ggacttgttt tagcaaaagg atattgtaac aataataacg
    23401 ctggctttgt caactttgat tgattgaatt cttctgtacg ggaattttct gcatcgtcca
    23461 atatggaagt gatgtaatct gacggaattt caaattgatg tgtcagctga tgaatctctt
    23521 cttgattagg acgttctaca caataccaga tcgtttggtc agtttccttt ccggtattac
    23581 gcagtttctc attatctaat gtaaaatatt tgatcatgat tttcctcctg ttgcctgttt
    23641 cttttattat aacggatagg gaattggaat acatggaatg tgtttgtaag atatagaata
    23701 taactgcata aatttaaata aaatgaaaca gttgtgtaaa gtatatacag tatacttgaa
    23761 gcaacgataa aaaaggagtt tacgttcatg ggcacattca caccacttat cttgttttgg
    23821 tatatttacc cggtaatcgt tttgtttggt tgccaatttc ttgtaaaatt attttcgtta
    23881 aatcgtcgtt ttaaaataaa agcccctgat ttggcgattc cttttttgtg gggcggtatg
    23941 catgcattgt ctagaaatac agggacaatt tctatcttac cgtttttctt tatttctgtt
    24001 ttgctaatgg gaatttgtat tgctgttttc caagcatatt attatgaaga aattatttat
    24061 ccgcgttatt tcaagatgac ttggcgtttg gtttttctgt tgacaatcgt cttgtacgct
    24121 gtcttgatca ttgtgaacat actttcttac ctatgaaaaa aatagaaaag ctgtaaaaag
    24181 tcttttaaaa ggctttttgc agcttgattt ttaaaaaaat gtttatgaat aaaaaaagtt
    24241 gttcccggat ttcccagaaa aggtggtaga aagtggggga ttgtggtaga ctaattttag
    24301 ttagtggagg aatggggggc tttcaatgtt gatgggtgaa ttccaacata atattgatgc
    24361 aaaaggacgt ttaatcgtgc cctcaaaact tcgtgaagaa cttggcgaaa agtttgttct
    24421 gacacgcgga ttggatggct gtcttttcgg ctatccgatg agcgaatggg aaaatctgga
    24481 agctaaactg aacgaaatgc cgttggctaa aaaagacgca cgtacttttg tgcgattctt
    24541 ctattcggct gccactgaat gcgaacttga taaacaagga cgaatcaata tacccagcac
    24601 gttaagaaat tatgcagctt tgacaaaagg ttgcgtagtc atcggtgtat ctaaccgaat
    24661 tgaaatttgg gatgagacac gatggcaaga tttttctgca gcagcagaag agaactttga
    24721 cgaaattgct gaaagtatga ttgatttcgg actataagaa taggaagagg ctgactaatg
    24781 actgaaacat ttcaacacta tacagtcatg ttgaaagaaa cggtagatgg attgaacatc
    24841 aaaccggatg gcgtttatgt agactgtaca ctcggcggtg ccggacatag tgaatattta
    24901 ttaagccaac taagttctaa agggcatctg tatgcgtttg accaagatca aaaagcaatc
    24961 gatcacgcca agctgcgttt agcttcttat gtcgaacaag gtatggtcac atttatcaaa
    25021 gcgaacttca gagaattaga agaacgcttg agtgagcatg tagataaggt agacggcatt
    25081 ttgtatgatc taggggtttc ctctcctcag ctagatgaag cagaacgtgg atttagttat
    25141 catcaagatg caccgttgga tatgcgcatg gatcagtcag ctcctttatc cgcttatcac
    25201 gtaattaatg aatattccta ccatgaatta gtaaagattt tcttccgcta tggcgaggag
    25261 aaattttcca aacaaattgc tcgtgaaatc gaacgagtac gaaaaacaaa accaatccaa
    25321 acaactggtg agcttgtcga aatcattaaa caagtcatcc cagctcccgc aagaagaaaa
    25381 ggcgggcatc ctgcaaaaag aatttttcaa gctgtgcgga ttgcagtaaa tgacgaactc
    25441 ggtgccgaag aggcctcttt agaacaggca atccggttat tgaaagtcaa tggaagaatc
    25501 agcgtaatca cattccattc attggaagac agaattgtca aaagtatgtt taaagaatat
    25561 agtacggtac aagatctacc accgggactt cctgtcatac ctgacgaatt ccaaccagaa
    25621 ctgaaagtca ttactcgtaa accaatcctt ccttcagagg aagagctaac agaaaacaat
    25681 cgatcacgca gtgcaaagtt acgtattgca gaaaaaataa gaatgaaaga ggagtagaat
    25741 gatggcagaa ttaaagaaaa tgcaagatta cgaatatgat ttacctgaat tggtggatca
    25801 gccggaacaa caaatgcctg attcttcttc gaatcaagaa atcattcttc cacaatcacc
    25861 ggcaaaacga ttaaagcaga tttctgcttt ggaaaaaatt gctgtggcaa gcgtggcgat
    25921 tgctattgtt gctttatgtg ttttaacagt tatgctgcgc acaaatatca gcggagctga
    25981 aaaagaaatc acgtcaatac aaacagaaat caatgataaa aagcaagaaa aaacgagttt
    26041 agaacaagaa aaaaacgaac tttctcgtac tgaacggatt aaacaaatcg cagaagaaaa
    26101 aggattgtct attaatgacg acaatttaag gaaagtgaag taaatgagtc ttaaaaataa
    26161 aattcggcgg tttatggaaa agaaaaatct aaatccgatg aacaatcgca aaaaagtagg
    26221 tatcatttta tttgctacga gtattggaat gttcttttta tttgccgtcc gtttctcgta
    26281 tatcgttatt ggaggtcatg ttgctggtac ctcattagct gaaaaaacaa aacagttgta
    26341 ccaaggaagt gaagtagtaa aagcaaaaag aggaacgatt tatgatcgta acggtgtggc
    26401 gctcgcagaa gatgcctcct cttattcaat taaagcgata ctctcaaaaa catatacctc
    26461 tggggataaa aaactgtatg ttgaagaaaa gaattttgat aaaatagcag aaatcctgca
    26521 taagaatctt tctatagaca aaaaagatgc actgaatatt ttagaggacg gcgcaaaaaa
    26581 agagctgtat caagttgaat ttggtagcta tgggaaaaat atctcccaag aaaccaagca
    26641 aaacatcgaa gcggacatga aaaaagaagg tgtcgctggt ctttattttg tcgaccatca
    26701 ggctcggatg tatccaaatg gcgtcttttc ttctcatttt attggatacg cagttccaga
    26761 caaagatgag aatggcttag ttggtaaact aggtttggag tctgcttaca atgatatctt
    26821 gagcggtaaa gatggtaaga ttatttatca aaaagataat tttcaaaatc ctctgccagg
    26881 tacagtggca gaagaagaaa aagctgtcga cggacaagat atctacacga ctttagacag
    26941 cagactacaa agttatttag aaacactgat ggatcaagta aatgaagaat atcagccaga
    27001 agaattgaca gcagtcctga tgaaagcaaa aaccggagaa atcttggcaa tgggacaacg
    27061 acctactttc aatcctgaaa caatggaagg attgactgga aaagatgcta tctggcgtaa
    27121 tttcttggta caagatagtt atgaaccagg atcaaccatg aaagttttca ctactgcagc
    27181 tgctatcgaa gaaggagaat tcaacgagaa tgaaaccttc caatctggaa aaatccaagt
    27241 agaagatgct acgatcaatg accatgactt tggtgaaaaa ggtgtgctga ccatgcgtca
    27301 agctctttca tggtcaagta acgtcgggat ggtcatactg gaacagcgat taggcggacg
    27361 atggtataac tatttacaaa aactgggatt tggacaaagt actcattctg gattagatga
    27421 tgaagtaaac ggtgctttac ctacattaaa tattgtcgac cgagcaatga gtgcttatgg
    27481 acaagcggta ggtgtgacga atttccaaat gatgaaagct ttcacttcta ttgcgaataa
    27541 cggtacaatg attcaacctc gatatatcag caaagtggtg gatccccaaa ctggagaaga
    27601 gcgaacaact caaacagaag ttctagggca acctttttca aaagaaacaa cagaaaaagt
    27661 ccgtgaatat atgagagatg ttgtcgaaag cgaaaactac ggtagtgctt atggtgtata
    27721 tagtgtacct ggatacaatg tatcggcaaa aaccggaaca gcccaaatcg cttctgatac
    27781 agggggatat caaacaggag atacagccta cctgtattcg attgtggaga tggttccttc
    27841 tgaagatcca gactatgtct tgtatctaac aatgaaacac cctaagacat atgatcgaat
    27901 ggcattggct aaaatcgcaa atccattgat gaaacgggca atggatttta aagaaactga
    27961 agaagattca gatacagaaa caaaaacaga aaaagtatcc gtagccgatt atcgtaatct
    28021 tgaagcggac gttgcagcag ctgatgctca aaaaagtgga ctgcaacctg tagtgatcgg
    28081 taatggtaaa aaagtacaaa aacaatctac tgccaacggt gatcagttga tctctggaga
    28141 aaaactgatc ctatatactg gtggtgataa gctgatgcct gacgtaacag gatggtcaaa
    28201 agcagatatc atgaagctgg ggaaaatcct cggtattgaa gttagttttg atggagatgg
    28261 ttattgtgtc aaacaagaac ttgctccata tgaaaaaatc actgaagaca aacttagttt
    28321 tactttagaa gaataagaag agaaatagga gagaaaagag catggaatgg acacaagcat
    28381 tgattcccat tgtcagcagt tgtgcaatga caattgccgc aatgccgcta tttattggtt
    28441 attttcaaat gaaaaagcaa ggtcaggcga ttcgtgagga gggaccaaag tggcacaata
    28501 gcaaagccgg aactcctaca atgggcggct tagtgtttct gatcggctct attttgacag
    28561 gaatttgggt tggagcttgg caaaaacagc taactcctac tcttttcatc ttgctttttg
    28621 tattagcgtt atacggagta atcggctttc ttgatgattt tataaaaata ttcaagaaaa
    28681 gaaacatggg actgaactcg aagcagaaat tgattggaca gattctaggg ggaatcatct
    28741 tttatcttgt ttaccgttct gaaggttatc caggtgtatt gaatttcttc ggactagatc
    28801 tacctttagg aatcatttat ggtattttcg ctctcttctg gcttgttgga ttttcaaatg
    28861 cagtaaatct gacagatggc atcgatggat tagttgctgg cttaggaagt atctcttttg
    28921 cagcttacgg tctaattgct tggcatcagc atcagtatga tgtattaatc atttgcttaa
    28981 gtgttttagg aggtttactg ggtttcttcg tctacaacag aaaacctgcc aagattttta
    29041 tgggagatgt cggttcatta gcgttgggcg gacttttagc ggcaatttct ataatgctaa
    29101 accaagaatg gacattgctt ttgattggtt tgatctatgt tatggaaaca gcaagtgtga
    29161 tgcttcaagt tacttccttt aagcttacag gaaaacgaat ttttaaaatg tctccaattc
    29221 atcatcactt tgaaatgtgc ggatggtcgg agtggaagat tgatacagtt ttttggttga
    29281 tcagcattgt gacttcgttg atcacattat ggatcgtttg gtaggagatg acgggaaatg
    29341 aaaaaaatat caacatatga aaataagaaa gtgcttgtct taggactcgc aaaaagcgga
    29401 gtaagtgcgg ccaaattatt acatgaattg ggcgcacttg tcaccgttaa tgatggaaaa
    29461 ccttttgacg aaaatccgga agcacaagag ctgctttctt tagggataaa agtaataaca
    29521 ggtagtcatc caatcgaatt gttggatgaa gaattttcat tgatggtcaa aaatccaggg
    29581 attccctaca ggcatccttt agtagcaaaa gcccaagaaa tgggtattcc ggtaattacg
    29641 gaagttgagc tagcttacga agtagcggaa tgcccaatta ttgggattac aggaacgaac
    29701 ggaaaaacaa cgacaacaac gatgacagga ctactactga atgcaggagc agatcaagga
    29761 attgcacgtt tagcgggtaa tattgggtat ccagcaagtg gtgtagcgca agaagcaaaa
    29821 tcagaagata aaattgtgat ggaattgtct agcttccaac tcatgggaat cacagatttc
    29881 cgccctcata tagcggtgat taccaatatc tatgaagcac atattgatta tcatggtaca
    29941 agaaaagaat atgtcaaagc taaatggaac cttcaaaaaa acatgacaga aaaagattat
    30001 ctgatcctta actggaatca gtcagaacta caagaattag cgcaaagaac aaaagcacgc
    30061 gtactgcctt tctcgacaaa agaagtgttg gaagacggtg tgtatgctga cgattactca
    30121 atatactata aaaaagaaaa aataatggaa atatctgaac ttggcgtacc aggtaagcat
    30181 aatgtagaaa atgcactagc tgctatttct gtagcaaaat tgtatggtat atcaaatgaa
    30241 gctatacgag aaacgttgca ttttttccat ggcgtaccac atcgtacgca atatgtagga
    30301 gaaatccaag gcagaaaatt ttataatgat tcaaaagcaa ccaatatttt agcgactaaa
    30361 atggctctaa gtggatttga aacgtctaag gtcgtgttat tggctggcgg attagacaga
    30421 ggaaatacat ttgatgaatt aattccttca ttaaaaggaa tcaaagcaat ggtcgtattt
    30481 ggccaaacaa aagaaaagtt gatggatgct ggaaaaaaag caggcatcga gacaatagta
    30541 acagccgatt ctgtagaaca agcagttcct ttagcattag aaaacagtac agatggtgat
    30601 gtagttttat tatcaccagc gaatgccagc tgggatcaat acccaaattt tgaaactaga
    30661 ggaaacagat tcatggaagc tgtgaaccga ttaaaataga aaaagtagaa gcgagcgata
    30721 aaaaatgaaa attttggtta caggcggagg aacaggggga catatttatc cagctcttgc
    30781 ctttgtcaat tatgtaaaga ctcaagaacc gaatgctgaa ttcatgtatg tgggagcaaa
    30841 acgaggtcta gaaaacaaaa tcgtccctga tacaggaatg cctttccata ccttggagat
    30901 tcaaggattt aaacgaaaaa tttccatgca taacatcaag acggtccgat tgtttcttaa
    30961 gagtatccgt caagcaaaaa aaatcctcag tgattttcaa ccagatatcg ttatcggtac
    31021 tggaggatat gtttccggag cggtagtgta cgctgcagcc aaaatgggta ttccaacagt
    31081 tatccatgaa caaaacagtg taccaggtgt taccaataaa tttttaagcc gatatgtgga
    31141 tcggatcgct ctttcattta aagatgcggc ttcctttttt cctgaaaaca aagcagttct
    31201 tgtaggtaat cctcgagcac aagaagtagc agatactaaa aaatcagata ttttgaaaac
    31261 atttgatttg gatccagaaa agaaaacagt cttgattttt ggcggtagtc aaggagcact
    31321 taaaatcaat caagcagtat cagttttttt aagtgaatat attccttctt cttaccaagt
    31381 tttgtatgct tcaggagata gatattatga agagatccta aataaaataa atcctgaaat
    31441 acaaaatatt agtgtccagc cttatatcaa aaatatggca gaggtcatgg cttccagtga
    31501 tctgctggtg gggcgtgctg gtgcaacttc gatcgcagaa ttcacagctc tggggttacc
    31561 ggcagtgttg gttcccagcc catatgttac gaatgatcat cagactaaaa atgcaatgag
    31621 tcttgttcac gcaggagcag caaaaatgat tgcagataat gaactaacag gtgaatcact
    31681 aagtcagaca gtcaacgaaa tcatgggtga tgaagaattg caaaagcaaa tgtgccgagc
    31741 ttctaaagaa caaggaatcc ctgatgcttc aaaacgtctt tatgacttag taaaacaaat
    31801 aatcaaaaaa taaaggcggt ggtcttagtg agtaagaaaa aagaaccaaa aaaattgtct
    31861 catccgtctt ctgatgaaaa caatttgact ccttggcaaa aggctaaccg aaaatatctg
    31921 gcagaacaag agatgaaaaa ccaagagcca aaaaaagaag acataacatt agatacaccc
    31981 ctttcagatt ctgaagaaga aaaaacggtt caagaggaac agcaagaaga aagccagtcc
    32041 gagaaaaaaa tcgaattttt tgaagagatc aagcaaaaga aaaacggtgg accatataac
    32101 ggttcttttt tgaaccggct gcccaattta aaaagtcaac ggcacaaagt actatttcgt
    32161 cggttagctt tgatcatcac tgtattgggt ataccgctga tttttttgat ctattatgtt
    32221 tctccttata gcaaattaca agcagttgca gtatcaggca ataagacagt caacagccaa
    32281 gaaatcatct ctgatacaaa attgtcatta ggagaaaatg tttggggaca gtattttcat
    32341 cgatctactt atatcgatcg ccttaaaaaa gcacagccaa gaatcgaaac agcaaatata
    32401 catttcaaag ggatgaatga attcgatttg gatgtaacag aatataaaga gattgctttg
    32461 atcgcaaaaa acaatcaata ctacccagtg attgaaaatg gaacagtatt agatgaaaaa
    32521 gtagcaaatc ctacaaaaaa tctccctatc ttggaagagt tcaaagactc tgcaaaaatc
    32581 aaagaattaa caaagcaata caatcagtta tcatcagaat tacaaaaagc aatttctgaa
    32641 atcaagtaca cgccaagagc aagcaataaa aatctgatcc aattgaatat gaatgacggc
    32701 aatcaagtta ttgtaaatat caataatctt gcgaaccaaa tgaaatatta ttcgcaagta
    32761 gccaaagata tggatgaaaa aggtgtgatc gacatggaag ttggtatctt ctcgcatcct
    32821 tacggtagca ctcaagaaac ggaagaatcg ggccaatcgg aagaaacaac tgaaagtgaa
    32881 gaaaatgcgg atcaaacaaa cgaatcaagt gaagaggcag aaacgcaaaa agcacaaaca
    32941 cctggatcgt ctagcacaaa cggatcatct gattcaaatg attcaagtga aacaagtcaa
    33001 atagaagata atccaagtac aactccgtca agtttaaatg aaaattgata aaacaacttt
    33061 aaaaagtaag aatgtacttt ctaaaaacca gtaatttatg gtagaattgg aagcagtgaa
    33121 tatatctatt aataagtata ttttaaaacg gacgctagtt gttcgcttgt atatggaata
    33181 gtaagtagga gggaatccca ttcatggcaa aaacaggaat gtatgtaggc cttgatattg
    33241 gaacgacatc tgttaaagtt gtcgtggctg aatatatcga cagccaaatg aatattattg
    33301 gagtaggaaa cgcaaaatca gaagggatta accgaggcat tatcgttgat atcgataaaa
    33361 cggtccaagc gatacaacga gcagtacgac aagcagaaga aaaagcaggt atccagatca
    33421 aaggcgtaag tgtcggtttg ccagcaaaca tgcttgaagt agaaaattgc cagggaatga
    33481 ttgcagttaa cggagattca aaggaaatca ccgatgaaga tgtcagaaat gtggcttcag
    33541 ctgcccttgt tcgttcaata cctcctgaac gtcaaatcgt gtcaatattg ccacaagatt
    33601 ttactgtcga tgggttcgag ggaatcaaag atcctcgtgg aatgatcggc gtaagattag
    33661 aaatgtacgg tctgttgttt acaggaccta aaacgatcat ccataacata cgtaaatgcg
    33721 tggaaaatgc cggattaatc gtcaacgaaa tggtgattac tcctttagcg ctgactgaat
    33781 caattctttc tgacggagaa aaagattttg gtacgatcgt cattgatatg ggtggcggcc
    33841 aaacaacaac ggctgttatg catgataagc aattgaagtt taccaatctt gaccaagaag
    33901 gtggcgaatt tgtcactaaa gatatctcta ttgtattaaa tacgtcattt aacaatgcag
    33961 aagcgttgaa aataaactac ggggatgcat acccagaacg gacatcacca gatgaagaat
    34021 ttccagtaga tgtgattggt cagtcagaac cagtgaaagt cgatgaacgc tacttgtcag
    34081 aaattatttc agctcgaatg gaacagattt tcaacaaagc aaaagatgca ttggaccaaa
    34141 tcgaagcatt agagttgcca ggtggcgtcg tattgacagg cggagctgct agtcttcctg
    34201 gtgttgtgga cttggcacaa gaaatcttcg gcgtaaatgt aaaattgtat gttccaaatc
    34261 acatggggct gagaaacccg gtatttacaa atgtaataag tatcgtggat tattcagcaa
    34321 acttgagtga agtgtatcaa ttggcaaaaa ttgctgttac gggtgaaacc tcagcagctc
    34381 gtcaaatggt agttgaacaa gaaacaacaa acacatatga gagctatgaa gcacctgaag
    34441 aaacaatcta cgatgagccg gaaccaaaag agtccggaga aaatgtaaaa aataaaatca
    34501 agggtttctt tacaaatatt tttgattaat tcatcctggg aggaataaga tatggaattc
    34561 tcaattgata ataatatcaa tgacggcgca gtaatcaaag tcatcggagt cggtggtggc
    34621 ggtggaaatg ctgtaaaccg aatgattgaa gaaaacgtta aaggtgttga attcattaca
    34681 gccaacacag acgtgcaagc attaaaaaat tcaaaagctg aaacagttat ccaattaggc
    34741 cccaaatata ctcgtggttt aggtgctggt tctcagccag aagttggaca aaaagcggca
    34801 gaagaaagtg aacaatcctt acgcgaagct ttagacggtg cagatatgat tttcatcact
    34861 gcaggaatgg gtggaggaac cggtacaggt gctgcaccaa tcgttgcagg tatcgcgaaa
    34921 gaattgggcg cattgactgt tggtgtcgtg acacgtccat tcacttttga aggaccgaaa
    34981 cgtggccgtt ttgctgcaga aggtatcgct cgtttgaaag aaaatgtgga tacattatta
    35041 attatctcaa ataaccgttt attagaagtt gtagataaaa agacaccgat gcttgaagct
    35101 ttccgtgaag cagataatgt tttacgacaa ggtgtacaag gtatctctga tttgatcaca
    35161 gcgccaggtt acgttaacct tgactttgct gacgtgaaaa cagtgatgga aaaccaaggt
    35221 acagcattga tggggatcgg tgtggctagt ggagaagatc gcgtagtaga agcaacgaaa
    35281 aaagcaatct cctctccatt gcttgaaaca tctattgatg gagctgaaca agtacttctt
    35341 aacatcacag gcggtttgga tatgacattg ttcgaagcac aagatgcttc agatatcgta
    35401 gcaaatgcag caactggcga tgtaaatatc attctaggta catctatcaa tgaagaaatg
    35461 ggcgatgaaa tccgtgtgac ggtcatcgca acaggtatcg atgaatcaaa aaaggaacgc
    35521 aagtctagcc gaccagctag acaagcacaa atgcaatcac ctgctcaaaa gactgtatta
    35581 gatatggatc aagctaaacc aacttcttca gaagaagaaa acagttttgg cgattgggat
    35641 atcagacgtg aacaaaatgt tcgtcctaga gttgatgatt ccaattttga caatatcgaa
    35701 aaaaaagaat ttgatacatt caaccgcgaa gaaacaaaat ctcatggcga tgatgaatta
    35761 aatacgccac cgtttttccg tcgtaaaaaa taaaggagag gaagagcaca tgattgctga
    35821 caacttgcag aaaatcaagc aagaaattca ggattcgtgt gctcttgttc atcgtgatcc
    35881 gaaagaagtt acattagtag ctgtaacaaa atctgttgaa tccgctcaag ctgaagaatt
    35941 ggttagagct ggcgttatga attgtgcaga aaaccgagta gataaattat tagaaaagaa
    36001 acaagattta tttgattttc ctgaaataaa gtggcattta attggtaatt tacagcgcag
    36061 aaaggtaaaa ttgattgtaa atgaaattga ttactttcat gcgttggaca gtttaagatt
    36121 agcagaagaa atccagaagc ggttagagaa agaattatcc tgttttattg aagtaaatgt
    36181 ttctggtgag gaatcaaaac atggaatttc accagatgag cttttaccgt ttgtggaatc
    36241 tttagctgcc tgcacaaaaa tcaaggttgt tgggttgatg actatggcgc ctaaagaagc
    36301 ttctgatgag caaatccata aagtgtttgg tacgctgaaa gaattgcgtg atcttgttca
    36361 agcaaaaggt tatgcccatg caccatgtag tgaattaagt atggggatga gtcaagactt
    36421 tcaaattgcg attgaagaag gtgcaacatt tatccgtgta ggaacagcgc tattcaaaga
    36481 cgaataaagg aggaacaaaa atgccgcttt taaacagagg agcaattgct aattttttcg
    36541 gattagctga tgaggaagag tacgaatacc aagattatcc tgaaacaaca aaaaaggctg
    36601 tacagccaac taaaaaaaat acaacacaga atcaatcaac tcattctgta tctgctgcga
    36661 atactcatga aaaacaaaaa acggcgactt cacgtcctgt aacgcatgaa agtattcagc
    36721 cggctgaaaa gaaaaaagta attgaactac atcaatcacc tacaaaaact gtagcgaaag
    36781 aaagaacagc ttcaagaacc tctacacaac ctaaagcaaa tcttgctgga aaaatcactg
    36841 taatggaacc aagagcttat tctgaagcta tggcgattgc taaacgaatc attgctggag
    36901 aagcaacctt agtgaatttt catttagtag aagaaaaaca agcgagaaga atcgttgatt
    36961 ttctaacagg aacagtttat gcactagatg gagatatcaa acgtgttgga gatgagattt
    37021 ttctttgtac accgactggg atggagattg acagttcaac cgctcaatct tttgcagatg
    37081 cgaatttgtt tgatctgtaa gaggaggaac tacgatttat actattgcac tattacttca
    37141 tttacttcaa cgggcagcct atttctatag cattatcttg atggtatatg cattgctctc
    37201 ttggtttcca ggcgggtatc aatcagggct aggacgatta ttagctaaga tctgcgaacc
    37261 gtatatcagt ttgttcgatc gtctgccttt gaggattgga cccttggatt tcacaatttt
    37321 aggggcaatc ctgatcttga acttagccgt acaaggttta gcacagatcg ttatatggat
    37381 aactgtccat ttagtttatt gattgaagca acaaaatctt tattctcaga atagatagga
    37441 ggggaaaatt ttggatgcaa atgtatacca gcactttcgg gcagatgaac gtacatttat
    37501 cgattcggta ggcgattgga ttgaacaggt tcaaggacaa tatgcaccct atctgaccga
    37561 atttcttgac ccaagacaat cctatatttt agaaacactg attcgacaaa gcagtgattt
    37621 gcgtttcatg ttttatggcg gatatgaaca ggcagaaaga aaaagatgtt tgattttccc
    37681 cgattattac gaacctgtag aggaagattt tgaaacagca ttgtatgaaa tccattatcc
    37741 tagcaaattt gccagcctaa gtcatgggaa aatccttgga actttagtgg gaacggggat
    37801 caaacgctct tactttggcg atatcatctc ggacggtgat cgctggcaag tatttattgc
    37861 aaatgaggtg gatcattttg ttgctgcaca agtaacaaag attggaaagg ttgctgtacg
    37921 cttggaacca atcaaataca cagaaatgat tatgccgaaa gatagctgga cacaagaaag
    37981 aggaacagtt agttccatgc gactagacag tgtgatctct gagatttata atatttccag
    38041 acagcggtcc aagcagctga tcgaagctgg taaagtaaaa gtgaactgga cagaaaatac
    38101 aagacctgat tttgtacttg aactattaga tattatttca gtcagaggtt atggcagagt
    38161 ccagatccaa gaacttgagg ggaagacgaa aaaagaaaag tacagggtac tgttcggtgt
    38221 attacgtaaa taaaataaaa aggtggttta ctcatggctt taactccatt agatatccaa
    38281 aataagacat tcccgacaaa aatgcgggga tataatcaag acgaagtaga tgatttctta
    38341 gatttagttg ttcgtgacta tgaggaattg actcaaagaa atcgagaatt agaaaaagca
    38401 gtcaaacatt ctgaagaaaa acttgagtac ttcaatgaat taaaagatgc tttgaaccaa
    38461 tcaatcatcg tagctcaaga tacggctgat aaagtaaaaa caagtgcaag caaggaatcc
    38521 gaagtgatcg ttacttctgc acaaaacaaa gcagatgagt tagttgcgaa cgctgaaaaa
    38581 cgtgcacatc aattaacaac tgatgcagaa gaaaaagcaa gaaaaatctt gacagatgca
    38641 acagaaaaag caagacagct tgcgacagaa actgaagatc ttaagaaaaa aacacgagta
    38701 ttccatcaac gtatcagctt gatgcttgag tctcaattag aacaagtgaa aagtccggaa
    38761 tgggatgaaa ttttacaacc attctccagc tacgtgactg acagccatga agtgatcaaa
    38821 gaagttttag caaaacagct tgacaatgaa aatgatactg atgtaaactc agacacagga
    38881 gtcatagaag cacctatgac ggcatttgat acacaggcta ttgatatctc gatcgtgcct
    38941 ggtgaaaatg aaaacgagta gaacagaaaa gcactttgtt attcttaaag cgagccgatg
    39001 agagtggaag ttcggtaaga cactgcatgg tgaaagatcc tctacttgag acctgctttt
    39061 ccgaaattag agtaagaagc agcggctgac ctcgttattg gtcacagaga gtttgcacta
    39121 gatagatgca actaaatttg ggtggtacca cggcatgttc gtcccttgcg gctgaacatg
    39181 cttttttgtg tccggaaaac aaataaagga gtgtatggtt gaatgaaaat gaaagaaacc
    39241 ttgcaattag gaaaaacaaa attccctatg cgtggtaatt tgccaaaccg agaaggcgag
    39301 tggcaaaaag aatgggaaga aaatcgaatc taccaaaaaa gacaagagtt gaacgaagga
    39361 aaacctagct ttgttcttca tgatggacct ccatatgcca atggtaatat ccatttagga
    39421 cattcattga ataaaatcag taaagacatt atcattcgtt caaaatcaat gtctggctat
    39481 cgtgcaccat atgttccagg atgggataca catggattgc cgattgaaca agtattggct
    39541 aataaaggcg tgaaacgtaa agaaatgtcc atggcagaat acttggaaaa atgtcgtgaa
    39601 tatgcacttt cccaagtaga taagcaacga gcagatttca aacgcctagg agttgcagga
    39661 gattgggaac atccttatgt tacattagat ccttcttatg aagctgcaca agtacgcgta
    39721 tttggtaaaa tggctgaaaa aagctatatt tacaaaggat tgaaaccaat ttactggtct
    39781 ccgtcaagtg agtcttcttt agcggaagct gaaattgaat ataaagatgt aaaatctcca
    39841 tctatttttg tagctttcaa tgtcaaagac ggcaaagata ttcttgatga agatacagct
    39901 tttgtcattt ggacgacaac accatggaca ttgccagcaa accaaggtat tgctgtaaat
    39961 ccaaaattta cgtatgtatt ggtggaagcc gatggccgta agtttgtcgt agcgaaagat
    40021 ttgctagaaa cagtccaatc agaaatcggt tgggaaaatg taacagttct aaaagaattt
    40081 gctggacaag aaatggaata tatgactgca agtcatccat tctatgaacg tgaatcactt
    40141 gtgattttag gcgatcatgt tactttagac gctggtactg gattagtcca tactgctcca
    40201 ggacatgggg aagatgacta tatcgctggt agcaaatata aactatctgt agtgtcacct
    40261 gtagactcaa aaggattgtt tacagaagaa gcacctggat ttgaaggtat cttttatgac
    40321 aaagcaaatc ctatgatcac tgatctatta aaagaaaaag gagctttgct gaagctagat
    40381 ttctttactc atagttatcc tcacgattgg cggacgaaaa agcctgtgat ctatcgggca
    40441 actccacaat ggtttgcatc tatcgataaa ttccgtcaga atattttgga tgaagtagaa
    40501 aaagtagagt gggttatccc atggggtaaa acacgtttgt ataatatgat ccgtgaccgc
    40561 ggcgattggg taatttcccg tcagcgtgca tggggagtac cacttccgat tttctatgct
    40621 gaaaatggag aagcaatcat cacaccagaa acaatcgaac atgtggcaaa attatttgct
    40681 gaacatggat caattatctg gtttgaaaaa gaagcgaaag aacttcttcc tgaaggattt
    40741 acccacccag gttctcctaa tggcgaattt acaaaagaaa aagatatcat ggatgtctgg
    40801 tttgattctg gttcttctca tgaagctgtt ttacgtcagc gaccagaatt atcattccca
    40861 gcagatatgt atttagaagg atcagaccaa tatcgtggct ggttcaactc aagtatcacg
    40921 actagtgtgg ctatcaatgg tgtagcacca tacaaatcag ttatttctca aggatttgct
    40981 ttagatggtg aaggccgtaa gatgagtaaa tctttaggaa acatcattgc accagaaaaa
    41041 gtgatcaaac aatttggtgc agatatcctt cgcttatggg tatctagtgt agattatgaa
    41101 gcagatgttc gtgtttctat ggatatctta agtcaagtgg cagaagtata tcgtaaaatc
    41161 agaaatacga tgcgtttctt attagcgaat acagaagatt tcgatcctaa aaaagatact
    41221 gtttcgtaca atgatttacg ttctgtagac aaatatatga ccgttcgatt gaaccaagta
    41281 atcaaagaaa tccgagaaga agggtacgac aaatataact tcatgcatat ttaccgtaca
    41341 gttatgaact tcttgacggt tgatctatcg tcattctacc tggattttgc taaagatgta
    41401 gtttatattg aagcagaaga tgattatcaa cgtcgttgca tgcaaactgt tttctatcaa
    41461 acagctgtag ctttgacaaa attattgact cctatcattc cgcatacagc agaagaaatc
    41521 tggtcattcc tgaaagaaga agaggagtac gttcagttaa gcgagtttcc tggttatgaa
    41581 gagtttgcaa accaagaaga attgatggat acctggactg ctttcatgga cttccgtgat
    41641 aatgtcttaa aagcattgga agaagcaaga aacagcaaat tgattggtaa atctttggaa
    41701 gcaaaactaa ctgtttatcc aaaacaacaa gttcgtgaaa tgttaaaagc tttggatgca
    41761 gatattgcac aattattgat cgtttcacca gactactttg aagttatggc agcagatgaa
    41821 caagtgccag ataacgctat ggtatttgat gatgttgcta ttacagtgga aaaagcagac
    41881 ggggaaactt gcgatcgctg tcgccaagtt cgtaaagatg taggagtaga tgaaaaactt
    41941 cctcatctat gcggtcgttg tgcaaaaatc gttgaagact tctatcctga agctgtagct
    42001 gaaggattcg aagaaaagta aaagggaaaa gggggtgtga catgttcttt gtcacacccc
    42061 cttttgttat caatcgttta attttccacg ttcacgatac cagttatctg gttgccagat
    42121 ccattcgaat ccgtcttttt caagtagttc gaaagctgct ttaggtccca ttgatccagc
    42181 tttgtagttg ggaaaatccg gttgtgtttg gtcccaagct tttcgaatga catccacgat
    42241 atgccaagat tgagcgactt cttcccaatg agagaagttc gttccatcac cattcaacgc
    42301 atctaatagt aatttttcgt aagcttctgg gctattttca accatttctg cactatttct
    42361 aaattccaat ttgattggtt ccgtttcaaa tccttgccct acttctttcc cattcaagga
    42421 taaagaaaat ccttcagttg gctgaatata gatcgttaga acattaggtg gaagtgtcgt
    42481 atcgtcacat ggttcatcga cagatgtttt gaatacatta acaggaactt gtttgaatac
    42541 gatgttgatc cgagtccctt tttcagtcaa acgtttgcct gtacgtacat agaaaggaac
    42601 acctgaccaa cggaagttat cgatagtgaa tttgcctgcg acgaatgttt cagtctctga
    42661 ttgagcatcc actttgtctt cttcgcgata gcccttgaat tgtacaccat ccaattcacc
    42721 ttgagcatat tgcccgcgaa cgaagttttc taacacttct tcttcgttat aaatgcggat
    42781 agctttcaat gctttgatct tttctgtacg gatttctttt tcggaaaatg ctactggcgg
    42841 ttccattgca agtaatgcta caacctgtaa aatatgattt tgcaccatgt ctttaagtgc
    42901 gccactatga tcgtagtagc ccccgcgatc ttctacaccg aggttttcgc caaaagtaat
    42961 ttggatattg tcgatatagc gattgttcca ttgagcctca aaaatattat tggcaaaacg
    43021 gatagctgag atgttttgga tcatttcttt tcctaaataa tgatcaatcc tgaaaatatc
    43081 ttgttctggg aatacttctc tgatttgctg gttcaattca aaagcagact ggtaatcaga
    43141 accgaacggt ttttctatga tcaaacgatt gaagccattt tttgtcataa tattttgtga
    43201 tttcaaatga gaaacgatcg ttccaaaaaa ttgaggtgcc atagccaaat aatagacacg
    43261 gttaccgttt aattgatagt gtccatctaa tcggtcagac aattctttta atgtctgata
    43321 atgcgttgaa tcatttacat catgggattg atagtaaaaa tggcttgcaa aggtagttgc
    43381 ttcttcatct gaaggattca atgcacggat tgtttctttg acaacttcac ggtagtgctc
    43441 atcgctccag ggacggcgag cggttccgat tacagcaaaa tgctcgccta aatttccttt
    43501 tctgtaaagg cggaacaaag acgggtacaa tttacgctgg gctaggtcac ctgtaccgcc
    43561 gaatatcgta aacaatacat ttttttcatt agacatgtgg atacactcct ttatatttta
    43621 tcgacaaaaa tcgtgctggc cgccttaaag ctgacgggga ttttctttct atcattttgg
    43681 atagtgatcg gtccttcata agctgcgatg tcaacgatct catattcttc gttgatattc
    43741 aggtcaatag tgactaagta atccaacaat tctttttcat caagcactcg tgcgatacgc
    43801 acttttgtac cgacaggata ggactccaat gtttgtcggc gttcttcatg gacaacttcc
    43861 tcttttttag gaatcgatcc tccatgagga caatgttcag gatgattcaa atagttttct
    43921 aaatgatctg caagatgttc cgaagtcaca tgttccaata cttcagcatc atcatggact
    43981 tcattccagc tatagtctaa atgttcaact aaaaaaactt cccataagcg atgtttgcga
    44041 atcagactgc tagctttttg cagtccgctt gcagtcaatt gtacaccttg ataaggggaa
    44101 tgttctacaa gctcttcttt cacaagttta gaaatcattt cactgacaga tgcagctgag
    44161 acatctagac tagatacgat ttgtttattg ttgactttcg ttgtatctcc acctagttct
    44221 aaaatgattt tcaaataatc ttctcgattt ggtgtcatat tcgatcatcc tttcatggta
    44281 aaaaaccatc attagtaagt gtaacaaaaa aaacgttaat tgactatttt tctaaataaa
    44341 aaacaaagga tttttagacg tttttacaac aaaaaacgaa agatgataga gaagactcat
    44401 tctttcgttt ataaaggata tatatataca ttgggaggga tctatttttc agataacaac
    44461 ttatatgaag tgtctgcaga ttcgtcataa agagtttgtt cgggataaga aaagataaga
    44521 taaaaaccat actgatcacg tacgactaaa cgatattcat ctatttctac cacagatgtg
    44581 ccaagagact gaccgttgat caaaaacgtg taatcagatg taatctgtaa ttgttgtttt
    44641 tgctggctat cactaaagaa ccatgtacct tgatactttg agagtccttc tgacttttcc
    44701 gcttgttttt tctttttcaa gtaaataaaa cttgagagaa cggtaatgag tgaaccaaga
    44761 aaatagatca ttttttttat caccaaatca ctcctttatg agaaaataat cgcatagaca
    44821 tattctttct tctttatttc attgtaccga ataaaagcta aaatttctct aagactttcg
    44881 actggttttt ttactgtttt tctacaaatt atgataggat acatagtagg aagaaaggtg
    44941 acataatttg aagtatcagc aagtggaaat agcttttttt attgaccgcc cgaatcggtt
    45001 tatagcacat tgtatgaaaa aagacggaga aataattatt gcgcatgtca aaaatacagg
    45061 tagaggaaaa gaggtcctga taccaggcgc tgaagtggct attgtatttg ctcctggacc
    45121 taaacggaaa accgcctatg atctgattgc tgtaaaaaag aagggtgact ggtttaatat
    45181 cgatagtcaa ttgcctaatc ggctggcaat tgatggtatt ttagacggca cgatcctttt
    45241 accaggatta actggtccat ttacggtata caagcgtgaa gtaacttatc ttcactccaa
    45301 atttgatatt tatttagaga cggtggtcgg acaaaaagca tttgttgaag tcaaaggtat
    45361 gacacttgaa aataaagcga ttggtgcttt tcctgatgcg cccacactca gaggattgaa
    45421 acatatagga gaattaacgt atgccgcaca agatggttat gccgcttatg tcttgtttat
    45481 tgctcagttc gaacatcttc atctggcaac gatccatgaa gaaatgcaac cagcactagc
    45541 tgatatggtt cgacatgcgc agcaaagcgg agttcaaatc ctcgcctata attgtcaagt
    45601 atcggcagat gaggtaactg tcaagcaggc cattccattt gatgtagata tcccttttga
    45661 agatcctata caatagaaag aaggtaaaat aatgatcaac ttaggtatta tcggaacaaa
    45721 ttggattact catcaatttg tccaagcagc tttaagcaca aaaaaatatg aattgacagc
    45781 ggtatattct agacatttgg aaaaagcaca ggaatttggt tctcattatg aaggggatat
    45841 cgaattcgca acagatttga agactttttt caatattact catatggaca ccatttatat
    45901 tgcttctccg aatagtcttc actttagtca agctaagcaa gcaattttgg caggaaaaaa
    45961 tgtgatagtt gaaaagccag ctttttcgac gccaaaagaa atggatgaaa tcatccattt
    46021 ggcaaatgaa cacaatgtgt atttttttga agcagcaaga aatatccatg aatcaggttt
    46081 taaaaagatt gccgattact taccattaaa agacgagata atcggtgcaa atttcagcta
    46141 tatgaaatat tcttcgcgct atgaccaagt attagatgga gaagaaccga atatcttctc
    46201 gactcatttc tcaggtggtg cattgatgga tttaggtgtt taccttgtat atgcagctgt
    46261 tgcttggttt ggaatgccaa aagaagtcca ttatttccca agaaagattt ctactggtgt
    46321 agatggtctt ggatggggga tcttacgtta tgagaatttt gatgtatcga tccagccagg
    46381 taaaatcggt gattcttatt taccgtcaga gatttatttt gactcaggaa cgctagtgat
    46441 gaatggagtc aatgcaattg aacaagcaca atttcatgat cgcgcgcata aagaaatcat
    46501 tgatttggcg gtaacagctg cagaaaatcc aatggcagat gaagcagagg attttgccaa
    46561 tgtcatggct catccgaaag atccagcttg gggattgttg tatgaagagt ggatcgagct
    46621 agctagaaat gtgaaccaag tgatttatga tttaagaaaa aatggcggca tcaaattcga
    46681 tgcagataat gaggaagatt tttaatgaac gaatttgtac aagtatttcc cgaaaattgg
    46741 cagaaaaaat atgaggataa agggtttatc tcaccttctc ttattcaacg agcagttttt
    46801 cagccactaa gtaataaaca aagtatagta gcggtttctc ctacaggttc aggtaaaaca
    46861 ctggcttatc tttggcctct actactaaac gtggaaccag gtgaagcaag tgcgttggtc
    46921 atttttgctt cttctcaaga actagctatt caagtagcag atgttgcacg tgaatgggga
    46981 aaagacaaag agttgaaagt ccaatctttg gtgggcggag ccaacgtcaa acgtcagatt
    47041 gagggtttga aggaaaaacc agaaatcttg attggtacac caggaagaat attggaattg
    47101 atgaaagcaa aaaaaataaa agcacatcaa gtgaaaacaa tggtatttga tgaagcagac
    47161 cagctatttg acgctgggaa cagccaaatc attgatcaaa ttcttcatca agctccaaca
    47221 gagtatcagc tagctttttt ttcagcgaca gcagatcgtt cccttgaaag cattgaaaaa
    47281 atcactggga aaacgtttga gacaattgac gtgacagcag aggacgattc tcgaaaagga
    47341 ctgagacatt actttttacg tgtaccgata cgaaagaaag atgactactt gagacgtctc
    47401 acgcatatag aagattttca tggtctgatt ttctttaatc aactgactga attaggcgta
    47461 atggaagaaa aattgctgta tagaggtgta ccggtaggaa gtctggcctc tgatcagaac
    47521 aagctactcc gaaaagcagc gctagaacaa ttcaaaaaga aaatggtcag agcacttttg
    47581 accacagatg tggcggctag aggtttagat attacaggtc ttccttatgt agtgaatgca
    47641 gaagttccgt taagtgaaga agcttatttg catcgttcgg gaagaactgg tcgaatgggt
    47701 aatgaaggaa tagtaattac actcgtacaa gataataatc taagagattt aaaacggctt
    47761 ttacgtccat caaatataac acttgaagag atttttttat acggaggtaa gctgcaaact
    47821 gagcagccaa ccaaagaaga tgcagctcct gcagtaaata aaaagtatgc gaaaactttt
    47881 tctggaaagg agcagaaccc agaatataac aaagaaaaaa taagaaaaaa agttaaaaat
    47941 aaatcaaaaa aagagagaaa taaaggagca aggcgcaaat aagcgccttt ttatgctatg
    48001 attatgaaaa cgggaacaaa attggttgcc agaggagggg ttatgtgaaa gaacgtcaag
    48061 aagagatctt gcagctattg acagaaaacc aacaaggatt gacagcaagt gatgtagctg
    48121 agcgcttaac tatcgatcga agcaatgcaa gccgatattt aagtgaacta tacaaggccc
    48181 atcatattgt aaaaaccgct ggccgaccag tcgtttactc tttgccaacg gaaaagtcaa
    48241 aatctgatga agtgcatgtc gactcttcta cgcaagtgac ttttgaaaca ctcgttggag
    48301 aaaatgattc tttaaaagtc agcatacagc aggcaaaagc tgcgattctt tatccaccgc
    48361 gggggttgca tacgattatt tttggagaaa ctggtacagg gaagtcgatg tttgctgaat
    48421 gtatgtatca ttttgcaatc gattcagaga tgctttctgc agatgcgcca tttgtttctt
    48481 tcaattgtgc ggattatgct caaaatccgc aactgctctt tggacacatc tttgggatca
    48541 aaaaaggggc ttacacagga gcagctcaag atagtcccgg tctaatagcc aaagcagacg
    48601 gcggtatatt gtttttagat gagatccacc gtttaccgcc ggaagggcag gaaatgctgt
    48661 ttactttcat tgataaaggt atttaccgtc cgcttggaga aagtgcacaa gtacatgaag
    48721 catctgttca gatcattggg gcgacgactg aatcttcaga aagtttctta tctacgttca
    48781 atcgacggat tcctatggca atcaccttgc cgaatcttgc ttcacgttca ctggatgagc
    48841 gttatgaaat catctctctt tttatcaaac aagaagcgaa tcgtttgaat caacgtatcg
    48901 atatcgaaaa agaagcgatt cttgccttca tgctttatga tgcggaagga aatatcggtc
    48961 aagtgaaacg agacttgaaa ctagtctgtg caaaatcttt tttacattac cgtactcatc
    49021 atgagaaaaa gctaatcatt cgaaaagaag aactttccct tcaagtacaa aaaggattac
    49081 tgaaaatcaa ggaaataccg gaacgtctcg accgattcat ggacagcaaa agtcagtatt
    49141 tgacatttga accaggattt gctgatgttg tctggtcaca agatccagaa cgaaacatgc
    49201 aggtctataa cgatattgag gaaaaagtca cttctctttc cgaaaccggt gtggagaaca
    49261 ttgatctaga gaccctcatt tcaaaagatg tggatgctta tttccagaca tatgtcaaag
    49321 aattgactaa aagttcgatt cccaaagatc ttttgccaga tgaaatctgg cggttaacca
    49381 atcaattgta tgatgtggct gaaaaacggt tagaccgtat ttataatgaa aaagctcgtt
    49441 ttgcttttgc tttgcatctg caaagtacat tggatcgtgt gagagaaggt catatgatcg
    49501 tccacccaga tttgaataac gtacggaaaa attttaagag ggaattccag gtagcaatcg
    49561 atctttcaac gatcattgaa gaagaagaac aagttgagat tccttttgat gaaatcggct
    49621 ttattagtat gtttctttct atcaatctag gagaaagtga gcctgttaaa gaaaacaaag
    49681 tagatgttct catattgatg cacggcagac agactgcttc aagcatgttg gagatggcac
    49741 aagaactatt aggaacaaaa gtagggaaag cttttaatat gccactggtt atggaagttc
    49801 aaacaatgta tgaagaagtg ttacaatatg tcaaaaacta tcaagagcat ttgacgaatg
    49861 gcctactctt gttaacagat atgggatcat taaacacctt cagtgatttg atttatgaag
    49921 agactggcat tcggacgaaa gctatcacga tgacaagtac catgattgtg atagaagcga
    49981 tacggatggc aagtgttgga agaagcttag aagatattta tcaaaatatc cagctttcct
    50041 ttgaatcgat cgtacgtgat cagttcaaag cggaaaagtc tgaaacttta cagcgtaaaa
    50101 aagccgtaat cgtgacatgc tttactggtg aaggagtagc tgccaaatta tatcagcgta
    50161 ttatgccagt gattaatcaa aataaggtag aactgatcca gatgcagttt ttagaacgtg
    50221 aatcattcaa aaaacacatc gatgccttat tggaagaata tgagatcaaa gcaattgcgg
    50281 gaacagttga tgttgattat caaaatatcc catttttttc agcctatgac gtttttgatg
    50341 atgaaaaact aaatatttta aagcgtatag ctgatgatga gatcccgaca gctaaaattg
    50401 tcgattcact tgctggatca ttaagcgatg tcttttctat tgatgaactg atccgttcat
    50461 tgcaaaagac agttcagcag atccagaccg atttgtcgat tatcgtggaa cctggagtag
    50521 atactggaat cgttatccat ttagccttca tgttagacgg attgatcaaa caagaaagcc
    50581 atcggcaatt tgagaaactg tctattttct caaaaattta tcgtttggaa atggatatcg
    50641 ttcgggcaaa cttgatggca ctagaacgaa aatatcaggt aaaaattcca gaagatgata
    50701 tcgcatttct tacacaaatg tttcttgaaa ataaaataaa aaaatgaaca cactaatcat
    50761 actgtttaca cactaaacat ctgtgtgcgc actataaaaa agagctaaaa agcatgaaaa
    50821 cgctatttca gctctttttt ttattggcat gcttcttgcg ttatataaaa gtgtaaaaaa
    50881 ataaaaggag tgttaaaaat ggcaaaaaaa acaatcatgt tagtttgttc agcagggatg
    50941 agtacgagtt tattggtcac aaaaatgcaa aaagcagcag aagaaaaagg gatggaagcc
    51001 gatatttttg cggtctctgc ctcagatgca gataataatt tagaatctaa gaatgtcgat
    51061 gtattattat taggtcctca agtacgtttc atgaaagctc aatttgaaca aaaacttgcg
    51121 cctaaaggta ttccattaga tgtcatcaat atgcaagact atggcatgat gaacggagaa
    51181 aaagttcttg cacaagcaga aaatctaatg aaataaatca agtatgcagt tgggggtata
    51241 acaacgtgga agatcaacaa aatctagaag ccatcatggg gttaatcatg tttggcggta
    51301 atgcaaagag cgatgcaatg gaagcgattg cggctgctaa aatcggtgat tttgagttag
    51361 cggataggaa aatcgctgat gcagaagaat cacttataca agcgcatcat gcacaaacag
    51421 acatgttgac acaagaagca caaggaaatc atatgcaagt gacattattg actgttcaca
    51481 gtcaagatca cttaatgaca tctattgcgt ttactgattt agcaaaagaa atcatcgaac
    51541 tttatcgtcg cgtatacagc gaataaatac aacttctttt ttgaacggaa gaagaaaaat
    51601 ttttgaacgg attttttatt ttgttataag gagaactctc tttatataaa aaacttatag
    51661 gaggaagtaa aaaatgaacg gactatcaag ttggatggag aaatacatcc tgccggttgc
    51721 cgcaaaaatc ggagcgcaaa aacatttagt tgctttgcgt gatgctttta tcggcacact
    51781 gcccgcaaca atggcgggat cagtcgctgt aatgatcaat gcaattattc gtgatttacc
    51841 gcaacagttt aacagtacct atgctgacag tttagcagca gacaaaggaa tctttgccgt
    51901 attgaatgtc atcatcggaa tcaatggttt tgtctggaac ggtacactag caatcgccgg
    51961 attgatcttt gccttttctt gggggtataa tcttgcccga gcttatggag taaatgatct
    52021 agctgggggg atcgttggtt tagctacctt gattcaaggt atttcctttg gtccagcatt
    52081 atcagcagca ttagatgtga atgttccttc aaatgtcgca gatgcaatca atagttctga
    52141 gattggtgca acgattgcag atggcacact gtcagttgga ttatggggtt ggctaaaact
    52201 agatcatttg aatggaaacg cttattttac agttatgatc atgggagcta tttcagtcat
    52261 tattttttgt aaattgatgc ttgccaacat tacgatcaaa atgcctgatt cagtaccacc
    52321 agcagtatct aaagcatttg cagccatttt accagcgacg atcgctttat acgtcattgc
    52381 tattatcaat tatatcgtag gtcgtgtagc tgatgggcaa ctgatcattg atttggtgca
    52441 aaaatatatt gcagaaccat tcctaggatt atctcaaggg ttgggagcag tattgatcgt
    52501 cacagtattt gttcaagtat tctggttctt tggtattcat ggaccaaatg tattggcacc
    52561 agtgctagag ggaatctggg gacaagcaca actaatcaat attgatattt tccaaaaagg
    52621 ttataacgga aaaactggta caccagccgt gttagatgca atcgaagatg gaaaagctta
    52681 tatgtgggtc cgcggatcgt tcgatgcttt tgcatggttt ggcggatcag gcggaacgat
    52741 cgtcttactg atagctatct tgattttctc taaacgtgca gattatctaa cagtagctaa
    52801 attatccatt gggcctggta tcttcaatat caacgaacca gtgatgtttg gtctgccaat
    52861 cgtattgaat gcaatcatgt ttatcccatt cttgattgct ccagtagtag caacagcaat
    52921 tggatggaca gctacttatc ttggcctagt cgctccggta tcgcagcaag tcacatgggt
    52981 agtaccgccg ttcttattgt cattcttagc aacaggagcg gattggcggg caccgatcgt
    53041 aacattagta tgtatggtcg tgactttctt gatttgggca ccatttgtca tctctgcaaa
    53101 caaaatggac cctgccgcag gcgaacaaca ataagatgaa cgtgaggagc gggacgcttt
    53161 aagtttcgtt cctcataatg aattttcgag gaggaactga aatgattaca attgccagtg
    53221 caatgcaaca agatgaaatc aaagaattgt tagaaggata caatgaagaa ggaatgactt
    53281 ttacatttaa agaaaaacaa ggaatcaagc tgttctttga aacaagtgca gaagatccgg
    53341 aagttgcggc taaaaaagca aaggaactaa tcaaagcaca gccttgggga acagtccttt
    53401 attttcaatc aacagctgtt tagaaaatgg tttaaaaaga taggatggtt gagatgataa
    53461 aaaacggttt atttttatgt agtattggtt ctttgatcgt gttgtatagt ttgaatccac
    53521 attcgcaaag gtatgatttg tatagtatgg tcacaggtct ttttctaaca ggattaggaa
    53581 tctatttctt catgaagggg aaaaaaagag aagaagcaaa ggagaaaaat aactaatgga
    53641 aatcacgaaa aaattaaata ttccagcatc gtttttttac gataaagtta tggactctgt
    53701 ccttttcgat gtacgtaaag cgaccggcaa atcagtaact agaaaacagc tgaaaaattt
    53761 ggagtacgtc aaacaattct cgcaaaataa ccgggctcgc ataaaaatcg aagaagtcat
    53821 tgaaaaccag tcttataagt ttcgtacatc gacaacgaag aatgaattcg tcgttcatta
    53881 tcaaatcaaa ccattggacg attcgacctg tgaggttcat tacacagaaa aaatggagtc
    53941 ttatggcatg cttcaaaagt tgaatgatat gcttttagga acaatcttga tgtattttaa
    54001 acggagacgt ttcaaaaaaa tgctagatat gatcgaagca acgtattaaa tcaatgaaac
    54061 atggtactat ttagttattg aaaaatgaag taagctagaa ggaatttttc ataggaagat
    54121 agatctttcc tgcgaatcct cactagcttt tttgtaggaa ggggcagcaa aatgacaaac
    54181 aaagtaagag agctaggtgt atcttcgatt gaggaaatgt ttgatttagc cacttatgca
    54241 tttaatgggg cagatacacc tgaaaggcga gaacgttttc gtacacttgc ggagaattct
    54301 tggaattacg gattttttga tgaggaggat cggctaacaa gtcaagtcat ggcgacccct
    54361 tttccagtga atttttacgg acaagagtac ctaatggccg gcattggcta tgttgcttct
    54421 tatccagaag caaggggaca aggcgggatc aatcagatca tggaaaagat ccttgaagat
    54481 tgccgtgatc gtaaagtaag cttatcttat ctggcgccat tctcctatcc cttctatcgg
    54541 cgctatggct atgaacaatc atttgataaa atcagttatc aactaaacag tcgtgatgtg
    54601 ccgtttatca aaaaaaaatc tggaaaaata aagcgtatgg attttgaaga tgcaaaagct
    54661 gcaatcagaa gaatttatga acagatgccg gaaaaccaac gagctggtct gaagcgcgaa
    54721 gactggtggt atacgtataa attcaagcaa aaaggcaatt accagtttgc tttgcatttt
    54781 gatgataatg aggaagcaac gggatatatc gtttatcagc ttgcaacgtc ttcctttatt
    54841 attgcggagt ggggattttt ggatcatgat gctttttgca gccttgttcg cttcgtaagt
    54901 tctcacaatg gtgcttttga aacatttgaa tattcatgcg gctacggcgg taatgatcaa
    54961 aattatctat tagaaaatcc atttgcttct gtaatgatca caccttatat gatggcgagg
    55021 atcgtagata tttctttgtt tttggaaaat tatccattta aaaagagaca agctgcattt
    55081 gccctacaaa tagaagaaga tcattatgcg aaatggaatg aaggaatcgt ggaagtaaca
    55141 atcgaaaatg gaaaaaatca tctctcaaaa gtagaacaaa caaaactccc aatcgttcga
    55201 ggaagtatcc aacatatcac gcagctatta ttagggtatc gtaaagtgga agagttggta
    55261 ttccaagaaa gaatacaggt gacaaaagaa tatgaagaaa cgttaagctc aatcttgcca
    55321 cagcaacagc caattcttaa tgactatttt tgataaaaaa cgggtgatga caatattttt
    55381 gtcaccaccc ttttgttttt catttggcat ttgaaggaaa atgttaaagt aaatcctctt
    55441 ttttcgtaaa tttgaatttt ttgttttctt ttgttttagg gttttctttt ttgtggtctt
    55501 ggattgcttc tttcgtaatc ttttcccgat tttttttaga tgaatctaag cgttctgcgt
    55561 atactttttc ttttggctta tcattcattg gaaaggacct ccctacatta tttcattcta
    55621 cctctagttt atcatctgtt aaggactagt ggaaacgttt tgcctaactt ataagctgat
    55681 ttttttagca gataagcttg caagtaaaaa acaaacaagc tacaattgat ttattgaaat
    55741 tgttcctgta gctcagtagg atagagcaat cgcctcctaa gcgataggtc gctggttcaa
    55801 ttccagtcag gaacgtataa ataacccctg tatcctttgc ggtacagggg tttgttttgt
    55861 atttgtgcca aaaatgtgcc aaactagtta tcatttttat ataaactatc gaacacagta
    55921 ttgatttttt ctttatcctg atcttcaagc tctcgaacaa tatgaagata agtagacatt
    55981 gtagtttcta aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga
    56041 taaagtaaaa tagatgcatg tgtatgcctt agtccatgca aagtaaattg cttttcaatc
    56101 cctaattctt ttaatttttt gcgcaaatat ttgctgacgg cgtttgatga aactaatcca
    56161 ttttttaagt tgaaaaaaac aaaattatga gggtttttga tttcaaaatt ctcgaataat
    56221 tctttttgat tgattttaaa cttttttaag agatcaatcg tatgtttatc aatggaaatt
    56281 ttcctatttg aagtttcgtt ttttgttttt ccccattcat ttaatttata attccatgtt
    56341 ttattaatag tgatgatttg atcttcgaaa tcgatatctt cccatgttaa tcctagaagt
    56401 tcagcaaatc gcattccagt ggcaccagca actaacacga gcataggaga agagtactgc
    56461 gcagaaaggt tttcttctac gagtttcatt aaagatttaa attgatcata atctaaatat
    56521 ttatcttctt ttggcttcaa tgaatctttt cctttgatta ctgcttttct agttggatca
    56581 aacggaatta atccttcttc tacggcatct ttcaacgacg ctctaatatg gttattaaac
    56641 ttaataacgg tagattttac atggtcttta gcatacttat ttaaaaagcg ttgatatccc
    56701 actctatcta aatcagaaat caataccgct ggcatatatt tctttatgtt cattaacgta
    56761 tcttcgtatt ttctataagt gataggagat actgttcctt ctttataaag ttgcatccaa
    56821 tcctcaaagt aatctgataa taatagattt ttgcgatccc ctttaaggcc tttgttcaag
    56881 ttatattcta attcgttggc ggcatctttt gcttctcctt ttgtcttaaa tcctgatttt
    56941 cttatcttcg tgtattttcc gtcatctttt ttgtatgaaa tttcgtattg ccaactattt
    57001 ccacgtttga ctaatcttgc cataattgaa tcagctctct ttctctgata caataggaac
    57061 tataaagaag cccattgttt gggttttatt attttttgca catccttttt gcttgccggc
    57121 gcaaggatgt gctatttgtc gttttaatgg tataattgga tgtgtattag atcatccaat
    57181 cagaggtgtt tattatgatt aaagtgattt ttaaaagaat aactgaactt ccagagttta
    57241 gattatttgt tgattattgg tatgtctggt tttttattgt tgcattgctg ataggtctag
    57301 atgtcatatt agaaagaaaa cttaagtaag agttgagtta ataaattgaa ttcctaaatg
    57361 agatattttg tagcccatat tttgtttgcc gacatcacct aatactatgg agctaatcgt
    57421 tttttctaac agaccaaaat cagataatct atttaagcta ccatctatta gctcttgtga
    57481 atagccagac atgctgatat ttccagtgaa attttcgttt ccacgactca ttaagcatgc
    57541 taacaattga atctcaatcg atgttaattg attcagtaca agaagataat actcctcgtt
    57601 atcccatgaa gcgttgttaa ttcgaagtaa acaattctta taaagatttt tatacatatc
    57661 tattttattt tgtgcctttg atttttctac ctcgtcattt atcccttcga atattccaat
    57721 gagttggtcc ttgttctcta agttgaaaac atcttctgga atttgatcgc gaatgttttc
    57781 taatgattcg tttaaatttt tgtagaattt ctcgatgcgt ttgaaacgct tctcgttttt
    57841 agacccaaaa taaagtgact gcacaacgcc accaatataa ggaattgctc caataccgcc
    57901 ttcaactatt aattcaagat attctttttt ggttagtttt tcattgctat tatccatagt
    57961 tacctcctat tttgtcattt cctttacaag cattgtgact cctgatggca ctcttttgta
    58021 taggttttat tattgtgcac aatctaactc ttagtggggc gtggttcatt tattttttac
    58081 ttactttact attaatcata tcccattgag cataggattt ttctatgttc gatcctcttg
    58141 aactaatttc tcctgtaaaa gttactttat cgccctttgt aaaggtatca gcattgttta
    58201 agtccttcgc aaaaattaca tatgaaactt taggtgttgc agaaatatca ttccaacttt
    58261 tatcatcgta ctctttgtct gctataatgg ctattctagt agtcattgat tcgataacag
    58321 ttccttcaaa agtatatgtt ttaccactaa tatatttagt aaaagctgta ttttgctcag
    58381 tagctggagt aatagaataa taactatcta agaaagcacc ccagtcatga ttatttgtct
    58441 ctaaaaattc ttcaaattga gttaggcttt tattgctgct gttagatgta gtagtttgag
    58501 tatcagatga tgagctttcc tgtttgttat tgtcatttgc ctcgtttgat gaggaaagcg
    58561 agaaacctac ggtagtaaaa ataatcccga taataaggag aatcataggt gttttcttat
    58621 ttgttttttt gataaatctt acaattaaca tgattaaacc tataataaat aaaacaatac
    58681 cagtaaaacc aaatataccg tccattaata gatcctcttt ctcttttgat attgaatatt
    58741 ataaaccaag caattctttt ttcttaaaat cgaattcttc ttgattaata atcccttcat
    58801 ctaacaactc tttatacttt ttaatttcat ctgcacttga aatttgtgtt tctttggaat
    58861 tattatctaa tatagattgt tgcttctcta tatatttttt taaattttcc atttcattcc
    58921 aatatttttt tgtaaacata acggtgtttt catctttcgt ggccgctaat atacctccgc
    58981 gactttcgtt tccgcctagc aaggtaaatt gtatataacc attactcata cctggttttt
    59041 ttaattgtac ggctgatata tttttaaagg ggatagtttt ttctccttta ataccttgat
    59101 taactaaatt tataaatcct tttctaataa ttgaaataga atcatcagtt actttaatca
    59161 aggtttttcc tggagatttt attaaaatct ctttttccat tttttcttcc tcatttctgt
    59221 gatatgattt tttttgtaga agctcatata tgaggaaaga gcccgtgttg cagcacgggc
    59281 ttttttaatg ttttgctgaa atcggttttt ttaataattc ttgatgtatt catacatatt
    59341 gccttgcgta agaatatttt ttcttaaaat agcattggca gacagcataa caagtgtatc
    59401 tgagcttatt ctgaatagaa taatactcca taaatttttc gagattaaac tgagattcat
    59461 cagtcagttc gttctcaata tagatattta ataaaattag aatagctatt ttatctgctt
    59521 ctgtttcaaa ttttgagtga aaagttgtag aagtatcgta caaaactgaa aattcaaaat
    59581 gagaggcgct gaaatgtgca agttcatgag ataaatgaaa ggcttctgca gtttcaccgt
    59641 atagattttc attcaaaaaa atgattctgg gttttggata gtagaaacca ggctctttca
    59701 tctccatata aactactttc aaattgtatt cgctcaacat ttctttcaat ttcaaataca
    59761 tacaaaccat cactccaact attcattttc ctctaaagct ttagcaattg caatcgcttt
    59821 acgcattgtc tccttagata tttcttttcc atcaaaagaa aaaacagtat cgtcttctga
    59881 taaatccaca tgtttagggg tttctctttc ttctctacct agaaggtagt ctacagagac
    59941 atcgaaatag tcagcaatcc gacttaattc atctgaatta ggagtattat ttttccactt
    60001 tgctagataa ccgttagaat atccaaaatt aatttctaac tgccgtatag atatcttttt
    60061 cctcttcgcc aagtctttta ttatttcata ggtattcatc gataaaacaa cctttctaaa
    60121 tgtttacaaa aaaagtttag aaaaatacgc agaaataatt tgactaattc tgagtaatga
    60181 gctatactat atcttgtaaa cagatttaac aactaaaaag acaacaaaaa acactattga
    60241 tatgtaaatg cagaccgcca agaaagcttt aaaatcaatg tttttatgtc ttatttaatt
    60301 atgctcttag tatagagtat tactcagata ctgtcaattg aatttagaaa aaagttgtta
    60361 aatttgttta cgaatataaa agaaaggaat gagaaatatg aacacaccgc aaatttttaa
    60421 tttcgaacaa aacgaagttc ggacaatttt agtaaatgat gaaccatatt ttgtaggcaa
    60481 agacgttgcg agtgttttgg gttattcaaa cactaaagat gctttgtcgc ggcatgtaga
    60541 tttggaagat aagatggggt cgcgaatcac gacctcaggt caatcaagag agatgacaat
    60601 catcaacgaa tctggtttgt acagtttaat cttaaaatca aaacttccct ctgccaaaaa
    60661 atttaaacgt tgggtaacaa gtgaagtgtt gccagcaatt agaaaacatg gaggttatct
    60721 aactccagaa aaagtagaag aagctttgct taatccagat acaatcattc aattagcaac
    60781 tcaactaaaa gaagaaagaa ctggaagatt aatcgcagaa caaaagattg cagagtacga
    60841 acctaaaatc tcctatttag atagcatatt atcttctaca gattcagtaa caattagtca
    60901 gattgcagca gattatggga tgtctccaca acagatgaat aaattacttc ataaactagg
    60961 tattcagaaa aaagtcggta accaatggtt attgtgcaaa aaacacatga accaaggata
    61021 cacaaaatct catacaactg agatcccgaa agccgatggt ggcactaaaa ttgtaatgaa
    61081 taccaaatgg acacagaaag ggcgtctatt tatctacgaa ttactaaaaa aagaaggata
    61141 ttaccctcaa atggatttag aggaaattgg ttagaaagga gttttagtat gactgacatt
    61201 gcagaaatca ctcaacgaga tagagaaaaa atcaaagaat atgtcgaaag ttcaaagttc
    61261 ttaacttaca ccatgcttgc tgaaagattt ggaattagca aaagctactt atctttaatt
    61321 ttaaacggta aaaagacttc tgcagaagca aacagaatta tagattcgat tatcactatg
    61381 tacgaattgt aaagggagga acagctaatg caatatctag aagcaaaaat cccaattcca
    61441 gaaggctatg taattatctc ccaagtggat tatgaggagt taaaaaaagc tgataatact
    61501 ggtagatgga tgacgttgcc agaagtgcta gaacggatta acagaaaata tgattggttt
    61561 acttctagag ttttaaagaa cccaagatat agaaatatta tcgatatcga gaaaaacaaa
    61621 aatggatttg tctattatcc agttgaagga agagacacat atctattttt aagaagtaaa
    61681 acacttgaat ttttagaaac aaatttttcg gaaatcttaa ggaggcaagc ggatgggaaa
    61741 atttaataga gcgctagtgt tcagcgcacc gctaatcatc tacgctttag gactttgggg
    61801 aagcaggcaa gcgttgatag gaacgattgt ttacatggtt tggattttta tggggcttga
    61861 tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag ggggctgact aaaaatgaat
    61921 agaaaagaga aactagaatg tatattacta ttactcagtt taattctttc actaatttct
    61981 ctattgggaa gtttgtattt ttgatatcaa caattttgtg gatttgggtt ctatttagag
    62041 taaatatcat ttttgtttgg tgattaagga gtttattaaa attaattttc cctacttcaa
    62101 atgcaaggta acaagatata gcacttttag gaggaatatt tagcggtaca gcttcagagt
    62161 atattctatt atcatcgact gatcctcctt tagcaattaa tttcttaaat ggagtagctg
    62221 accatctgcg gtttaagtta gcggatgaaa gttctaaatt aactaacata gctggttctg
    62281 ttgaatagtt agaaatgatt acttttgtat aaatcatatc tgcagcgaag taagaagcat
    62341 taagttcaac ttctagttga gggcgcattc cttttatttt tatccctgtt aaaaacgtac
    62401 tcaatataaa acttattatt gacatccatt gaaaaattgt taagtgaaaa aaattcaaaa
    62461 taaacaccac cagtttttaa ctaaattata ccaaaaagga gagaagaaat aatgcaagaa
    62521 ttagtaattt tgaaaaataa agaagctgtg actacgagct tacaagtggc agaaagcttc
    62581 gagaaaaaac atcaacatgt tttaagagat attgatgcac taaaaaaaga tgtgtccaat
    62641 tttggacaga tgtttgtaga aggtaatgaa ccagattcat atggcagaaa tcgacgagtt
    62701 ttcttcatta gtagagatgg atttttcttg ctggctatgg ggtttacagg aaagaaagct
    62761 atctacttca aacaaaaata cattgaagca ttcaacgaaa tggaagatgt tattcgcaag
    62821 aatactgttc ctcaaacaat tgaagatatg atgatctatc aattagaaga aatgaaagat
    62881 gttaaaaaag atgtttccat gcttaaagat actatgcgaa ttagcggaca acaagagttt
    62941 gaaattaagc aaaaaggaaa tatgaaagtt atgggagttc tagggggaaa agaaagccga
    63001 gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc
    63061 ttttcaatcc caagatatgg cgagttacct cgtaagagat tcgatgatgc tgtttcattt
    63121 attgaaatgt ggttgccaga aactgcgatc cgtatggaaa ttgatcaact gaacagacaa
    63181 cagagacttt ttggtgatga caatgaatag agctgaagcg ctaagaatag ggacggcaat
    63241 tgctaattgc tggtggaaat actataaacc aatcatccta agccaacaac atattgacaa
    63301 gcaaaaagca tggcaacaaa taaaaaagtg actccgccgg caagcaaaga gtcacaaaga
    63361 aaacacatca taaggagatt ttagcatatg gaaaaagaac tttccactct agatcaatat
    63421 ttgattgatc ctgattgggg caagccgaaa attgaggaaa caagtggtcg aaaaatcaga
    63481 cgaaatcttt tgacgaatga agaactagct tgggatcaag atgatttagg caaccatgta
    63541 actatttggg atcatgttta tcttatccat ctatcgaagc attcgaataa acctgaatat
    63601 atttacgtca tcgaagatgg cttgattgat gcgctagaag agtacgacag agataacttg
    63661 attgatatct cttattacgg accaggtaag aaatacattg ctgaaatgga ggcagaattt
    63721 gatgagtgaa atcaaaggga caacgaactt tgaaaaactt tttagtcgta agttaaataa
    63781 aattctcaag aaaaaaggaa attttgatta tttatcttgg gctcacgcgt gggagattat
    63841 gaaaaagaat gatccacagg caacggtaac tattaatgag tacaaacact acagggttgt
    63901 ttctggaact catcaagact ttcttgttga ggaatataaa ccttttctta tggatgaaac
    63961 tgggacttat gtatctgtct cagtaacggt taaaggacac acggaaaccg aattatttcc
    64021 tgttttagat tatcgaaacc aaccagttgt taaaccaaat gcaatgcaaa tcaataactc
    64081 attgaagcga tgctttgtga aagcattggc tctacacgga ctgggattat atgtatttca
    64141 aggggaagat attccaacac cacctagaat cgatacaaag aaattaaaca tgctagagac
    64201 gattctagaa gctttcaatg agcagatggg taaagatatg accaaaacct taatcgaata
    64261 tgttaatgag cagacagata aattagggct cttagctgat aacgttgaaa ctattgaaca
    64321 gttaagctat gagcaatgtg ccttgatgga gcgagcaata gcagctaaga gaaaagaatt
    64381 agataagaag tgatatgagt gtttaaacca ttaatcgatt catattcagc ggttctgaaa
    64441 aagttcaaag gaaaagacat aagcgcaacc atcaatgagg aagtgaacat tgatcgacta
    64501 aagacgatgt atgacggcta cgatggtgat cgagtcattg aaattcgttt tattgatcct
    64561 agacgtttca ccgtacagca acgaaacttc atctatgcgc tgataggcga tatttttatc
    64621 gatacaggca tgccaacgga cttctggaag gaattcttct actttcgttt tgaaggtgtc
    64681 acagggcgca aaataagcct gaaagacgaa tcgaatacaa ctgtgagtga tgccaatgtc
    64741 ttagcaaata tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat
    64801 gagattttac ctgcgaatca agaatattac ttctacaaat gcatcacaaa aagagtctgc
    64861 tgcatctgtg gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga
    64921 aagcgcaaag aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac
    64981 acagagaagc acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc
    65041 aaattaaacc agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc
    65101 gaagttatta tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata
    65161 ctaagttgtt atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag
    65221 gcaatgagta ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa
    65281 gtgatttgat taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc
    65341 aaataatcaa taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc
    65401 ctattgacga aaaagtcaaa gataataata catctattaa taatacattt aataatacaa
    65461 aagaatatat aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata
    65521 aatacggaga gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag
    65581 aattccctaa tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg
    65641 gtaagactta taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg
    65701 aacctaagaa cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat
    65761 gggcaatcga ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat
    65821 caaaagcacc attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga
    65881 aatagcgaat tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact
    65941 cgaagaagct ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata
    66001 ctatttcgca ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact
    66061 atttgttgat tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc
    66121 agatggacct aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac
    66181 aaagtgtatc gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac
    66241 ttgatcaagt actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg
    66301 cagcgaatta tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca
    66361 gaagtgatga ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga
    66421 aaaatcgcaa tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca
    66481 agtctcacag gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac
    66541 gctaagaaga gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc
    66601 aaatgcgtat tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga
    66661 tggtcaatga aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa
    66721 tccatcgatt ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt
    66781 aaccaagtca gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg
    66841 taaatcaatg tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca
    66901 tgatgaccga attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag
    66961 caaaaacatt atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt
    67021 taggtatgtg gaggagagat aatggatctc attacacaat acagtgacat catcctcaag
    67081 aaaatcatga tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag
    67141 ttagaaatgg ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca
    67201 aacattgaat tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat
    67261 cggcaaacaa attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat
    67321 aaagaaatat tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt
    67381 cagcatggat ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct
    67441 aatcataaat tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt
    67501 tccagaaatt gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa
    67561 agtaatggaa ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg
    67621 tgggataaac gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc
    67681 aaaacaaact ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga
    67741 aacatcgacc tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa
    67801 ggggatatag tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt
    67861 attgtcaaaa attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta
    67921 gagtatcaaa tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat
    67981 atatacgaga atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa
    68041 agttagaaag tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta
    68101 taaattcagg tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac
    68161 aactcgtggc cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag
    68221 cgtatttaat caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag
    68281 atggcatagt gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat
    68341 atgaaacact attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa
    68401 taaagaatgc cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa
    68461 attatagttt tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg
    68521 atgctgcagc attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa
    68581 agacggattt tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa
    68641 gaaccctaaa acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc
    68701 agaagagttc gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat
    68761 cattcgtatt gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt
    68821 agctgtagcg agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg
    68881 ctccgactga ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact
    68941 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
    69001 tgaagaactt tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
    69061 cgattataac ggatatgccg aaggcaccga agcatggcaa taaggcagaa gacgcgatta
    69121 ttcagatgat ggatatagaa acggagagag atgcgattct agcggctttg atggctctta
    69181 gtctgattag tcgtcagata ctctactaca gcttctgtgt gccagatagc ttctcaaact
    69241 acagaattag ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag
    69301 ctctaataga gtttgcagaa gcatataaac acggaaaaat aattgcttat aaataatttg
    69361 gcggtttttt ggcggaatga tggcggtttt tagacattta ccagtgttat tatggtagtg
    69421 tcgaaagatt aggaaacagg atcgacaaaa taaacgaagg gaggcaatct ccctcatcgt
    69481 tttaaacttc ttcttagata gacagcaact aattatgaag aaaaggatgt gaattcaact
    69541 cctcctaaat tcttcatcgg ttgctgttta ttttgattag acagcagcac tcacaaaact
    69601 aaacatgttc ttgtcgtctt tcaactgctg ctgtctatta atttatgtat tggagggaag
    69661 aaaaatgaaa ttatcagtag aaggcactcc agaagaaata caagaattgc tccaagctat
    69721 tggtagtagc aaggagcaaa aggtaagtgt ttctaattca gatattgacg gtatttgcaa
    69781 ttgtttaaac agagaaaaaa tatctaatcc ttactaagag aaaatatgtt cgttaatgaa
    69841 tttccaattt tcctctgtag ctctaccttg ataatttttt tgcagttcac agatgaataa
    69901 ggaatcgtta ttatcaagta ctgtatttaa tttttcacac atttgatttg gattcaaatt
    69961 agttttaact aaccaaaaag atttttgtag ctttatatat gccccaaatt cttttattat
    70021 ttcaaatact ttatcatact tttgccctgg gttattaaga tcataactaa tcatgtaggg
    70081 tttatacatg ttgtcttgca catccttgtt ttatatttca gcggaccact cgctgataaa
    70141 taaaattata cgcttagtat ttattttcac aatattaatt tatcgtaata acttttgagt
    70201 aaatagaaca aaaaacctgc actagtttcc gctagtacag gcagtgacta tatcggttga
    70261 taatctagca tatattaaaa ataattgcaa gaaaagaaat ttatagtggt ttataccaaa
    70321 ttatcaaagc aatgttattt tgttgctgtc tattgttttt taattattca cacgataact
    70381 aaaggtgggt gaagagaaat gattccatta ataatttcaa tttttgcgct ctgtcttaat
    70441 gtctatatga ttggatttaa aaatgggcaa aataaaaaat agtagcagcc aagaataatt
    70501 ttatagtgtc actgtggcgg aaagggtaga cgctaagcat gtgtgctagg tcaatgcttc
    70561 ggcaaccatg caaggttcga ttccttgcca gcgacattaa atgcctatga cggttacgac
    70621 taccgaaaaa agatcgttaa gaagctatac ggtgctacgt acggcaatgt agtaagtgtg
    70681 ctatctgtac accaccaagc ttcggtcact gtggcggaag tagaagacgc agcggtaaat
    70741 ggcgagtagc ctcgtgagag cctggtaagt tctcgtgagt ggtgcaatcc cactccagcg
    70801 actttaagca accgaggcat cggcggttta aaaatatagg ggtgcgcaat ttcgtacgcg
    70861 ttttgtgcat cgtgcaagtt actattacat attagatcac tctttgagtg gtctttttat
    70921 ttttgcacaa aggaggaaac aacaatgtat agaccacaat acttagaaca gaagtatgaa
    70981 gtaatcactg ttcataaagg taatggcgaa aaagtatatg agtatagaag accaataaag
    71041 agcgatacat ataaacgaaa ggaaaacaat gaagttattc cattgtatgg caaaagaata
    71101 gctaagcatt aaataagatt gcgaaaggag acggaacatg accgaggaat tctatagatg
    71161 gctattacag ttgacaagag aagatcgttt ggttaagttc tatcagtctc ctaaatggcg
    71221 caggcttaga gagaaagcga tgaaacgaga tcactatgaa tgccaagagt gtagaagact
    71281 aggtaagtat catagagtag agaacgttca tcatataaag gaagtcaagg atagacctga
    71341 cttagcttta gatttagata atcttatttg tttatgtgtt gaacatcata atgaagttca
    71401 tggcagatat cttacagcat tagataaaca agagaagaag atagaaagct ttgctaactt
    71461 cgatgcaagt gaaaggtggt aagtgcatga tcatcaatga taatggcaga gagtatgata
    71521 cagaaaagat tgaagagtat tcatcttata ctcagggatt aattaaacgt ttgatatacg
    71581 ttcgctatgt aggtattagg gatctgttat cagataactg ttgtagtaaa tacaaagtga
    71641 atcaagtaag agaagcgttg aataaagata ataacgtcga aagaataaaa aatgtttttg
    71701 gatatagtat tgaagagatt aattattaca ttgacttcgc tgaagctttc attccgatgg
    71761 tgagataacc cccccttaaa ataaatcgca aattttttgg gggtgatgaa acggaggggg
    71821 ctgtcaggaa aagagatttt ttcgaacttt atcatgaaag gagggctaaa atgtttaaaa
    71881 acgaattgtc tcaaaatcgc tacagagaaa aattacgccg ctctttaata agccaattgg
    71941 aaagtcagaa aacaaatatt gagccattct tagataatgt tgatcgttat atcagtttat
    72001 gggaaacggc gatatcactg gaagaagata tatccgagaa cggcattaga ctggagaatg
    72061 gtaaaaagaa tgaatcagta gcgttgcttg tttctgtcaa caaacaaatg ggattgatgt
    72121 tggataaact tgccattact cctgaattgg taggtgaagc aaatgaatca attcctgagt
    72181 tataagcata ttgaaaattg gttcaaagct atagaagaag gcactatcaa ggtatgcaaa
    72241 gagcaattat tgctaaaaaa ttatctagaa gaaagagtct ttactagaga agatatttac
    72301 ttcgataagc agatggtaga ggattcaatc aatataccag cacaatactt tccattcgaa
    72361 ttaattccgt gggaaaaatt tctacaatgt tttatttatg gtgttcgatg gaaaaaagat
    72421 aaaacactag tgttcaatag atatctttca ttaatgggac gtggtaatgg taaaactggt
    72481 tttgcttctt ggaacaactt ctttctacta accgctaaac acggtattaa aaattatgat
    72541 attgatatct atgccaataa tgaaagccaa gcaaagacta gttttgatga tgtatttaaa
    72601 gtaattaaag atcatcctga tttagataaa aaagtattta aagctacgaa ggaagttatt
    72661 caaaatatcg ctacaaacag caaacttcgt tataacacgg caaatgctag aacaaaagat
    72721 gggaagcgac caggtgcaaa ccgctttgat gaaattcacg aaaatgaaga ttattcaatg
    72781 ataaatgtgg ctacttctgg tggtggtaaa attcgagatt atagagaatt ttatgataca
    72841 actaatggtc atgttcgtgg tggtccgctt gatgacatta tagaagaatc aaaaatgatt
    72901 ctttctggag aacttggaat tgacaaggat ggagcagaat tttctagttt gtttccattt
    72961 atttgtcgct tggataacga taatgaagtt gatgatcccg acatgtggga aaaagcttgt
    73021 ccaactatta attacaatgc agatttaaaa cggaaaatgt ttcaagaata ctctcaaatg
    73081 caacgtaatg ctggtttaag acttacgttc atgaccaaac gaatgaacag acctatggaa
    73141 gatacacgat ttgctgttgc ttcatatgat gatgttctgc atacgaaaga aaaagaattt
    73201 cctgaaaaaa tggatgaagt gataggaaca gtcgattttg ctgatagacg agattttgcc
    73261 agcgttgggt tgctaggaaa atacgataaa gatgtttatt ttacacaaca tacttttatc
    73321 cacgaatcag cccttcgatt acaaaacatc aaacgagagg ttatagatat ttctatagat
    73381 caaggaaaat cacagatcgt tcatggaaaa aatatagaag ctgattatat tgtaggttgg
    73441 tttcttgaaa tgagtaataa atattatatt aaaaaaatcg ctatggatat gtaccgtgca
    73501 aaaatattga agcccgcttt agaagaagca ggttttactg tggaaattgt tcgaagcgga
    73561 tctgttacac atggtatgtt aaaagatctg gttgatgacc tttttattaa tcaacgttta
    73621 ttttttggtg acgatgcgat tatgcgttgg tattgcatga atgtatatga agagcatatt
    73681 tctaatggaa atatacgcta tgaaaaaata gaacctgaaa ctagaaaaac ggatggcttt
    73741 ttttcattcc ttcatggttt gaatttttta gatgatattt atgattctgc tcctgtaaca
    73801 gtcacaaata gctcagtaga aaatacagga actggattta ctcctctagt attctaactt
    73861 gaaaggaggt gagaaagtgg ggatttttca aaaggcggta ggatacttca caaaaaaagc
    73921 aacggttcct ttagaagaat acttttgtaa attgcaagtt gattttgtgt atcgaaaatt
    73981 tgcgattgaa acttgtattg atttgattgc aaatgcgatg agtaaagcgg aattcaagtc
    74041 atatgaagat ggaaaaaata aaaagaatga tctttactat aggctgaatg tagctcctaa
    74101 taagaaaaat aatgcaacag aatttagaaa aaaactgatc aggagattaa tattctacaa
    74161 tgaagtattg atcgtttctc cgtctaataa ttctagcgaa atatttattg cggatagttg
    74221 ggatgtcaca gaatatgcat tgaaagatga tgtgttttct caagtgcaaa ttaacaacat
    74281 agtccttgat agagaatttc tagaaagtga tgttatctat ataaaatacg cagatcaaca
    74341 aattaggcaa ctagtcgatg cgtattatca agcgtatggg aaactcattt ctagtgccat
    74401 gaatgtttac aagcgctcta acgctcgtag atacgtactg aaagggaatt tattccgacc
    74461 gcaagacaat acaacacaag atcaaatcaa taaaatgatg acatcacaat ttaaggcttt
    74521 tatggaagct gataatgcag gtgcggtatt tcaattacaa aatgagtaca cattagaaga
    74581 tttcagcgga aacttccaaa gcaattcaag agatataaaa aacttaatag acgacatctt
    74641 tgagatgaca gcagcagcgt ttcacgttcc gaaaaaccta ctaaagggag acatgagtgg
    74701 gttatcggat caagtggacg cttttttaat gttcgaaatc ataccgattg ctgaacttat
    74761 tcaggatgcg tttaacgcta gtctctatga agtagaagaa tacttgtcag ggaattttgt
    74821 acgtgtggat acaactatga tcaagattac tagcttcaaa gatttggttg acgctattga
    74881 tgtaggcatt agaaatggag tatttacaat caacgaagga agagaacgcg ttggaaatga
    74941 tcgctctgat aaggcgatgg cagatgaaat atttataact aaaaacaatc aacaagtatc
    75001 gaaaggaggt gaggcgaatg acgacaatga aaacatttct agcagtaaag aatgaaggcg
    75061 cagtaccgca aatttttatt cagggattta ttggttctag ttggttcttt gaagggaata
    75121 ctgacaaggg aatcaaaaat attttggata gtctaggtga tcaagaagaa attgaagtag
    75181 taattaattc aaacggtgga gacgtatttc aagggattgc tattgggaac ttacttaagt
    75241 caaataaagc aaaagttaac gttgtgatta acggcttagc cgctagtgct gcttcaatta
    75301 tcgcaatggc tggcgatact ataaaaatct acaacaatgc acaattgatg attcaccgcg
    75361 cttccacata cggagaagga aatgtcgatg acttccgtac gattgctgac caactggaat
    75421 caattgataa atcggtaaag gcttcatata aaacacgatt caatggcaca gatgaagcat
    75481 tgcaagaact tcttgaaaaa gaatcgttta tggatgcaga aacagctttg agttatggat
    75541 tggtcgatga aattatcgat gcagaaaata gctcaggtac tgaagctaaa aaagaacaaa
    75601 gcgttgaaga aattttgaat gacgttaaag aaaaaagagc agaaaaaatt gctgcattta
    75661 cagcagcatt aaataaaaca tttggacaag gagatgtaaa ataatgacag ttaaaaattt
    75721 aaaaggtgta acagctgcaa gcgaccaatt gatgaaagct tttaaagatg gtaacgaaga
    75781 atcttttagc gcagctatgg taagcttatc taaggaaatt caggataaaa ttttagaaga
    75841 agcaacagca aaaaatcaag atcaattagt attaatgaac cgtggtcagc gtgtattaac
    75901 tacacaagaa acaaaattct ataacgaagt agtgaaaaac gaaggttttg caggggtcga
    75961 agaattagtg ccagctactg tatttgaacg tgtatttgaa gatttagaac aatctcatcc
    76021 actattgcaa aaaattactt ttgttaacac aactggtgta acagaatgga ttgtgtcacg
    76081 tggagtcaat ccagcatggt ggggtaaact ttgcgaagct gttaaaaaag ttttagataa
    76141 tggctttgat gtaattaaca tgaagcagtt caagctatca ggttatattc ctgtatgtaa
    76201 ggcaatgctt gatttaggtc cagtatggtt agatcgttat gtccgtactg ttttagtaga
    76261 atcattgaga attgcattag aacaagcaat tgttgatggt actggtaaag atatgccagt
    76321 cggaatgatg cgtgacatga gcaaacaaac tagcggagaa tatgctgaaa aaaaagcaga
    76381 acctattaca gctttagatg ctgtaactat gggcggtttg atggcgcgac tatcaaaatt
    76441 caatatcgaa ggtgtgaatg atccgattta tcgtaatgtg aatccttctg atgtggtcct
    76501 aattgtgaat ccaactgatt actggtctaa agtattccca gctaagactg tactaactgc
    76561 taatggagaa tacgtacaag tattgccagt accagtttca gatttgcagt caacggctgt
    76621 gccagaagga aaagcagtta ttggggtagc ctcagattac ttcatgggtg taggatctac
    76681 actaaaaatt gaagcttcag atgaatacca ttttgttgaa gacgaacgca tttatctagc
    76741 taaacaatat gcaaacggtc aacctaaacg taacgatagt ttcattgtat tagatattag
    76801 cgctttggga actactacta caactacaaa accaacaacc acaacaacta caacacaagc
    76861 gtaggtgatc agaatgaagt atattctttg tcagccggca atcaatcggt ttaaatggga
    76921 gcttgaagtt tgtttaacta atctgaagaa actaggaatc aaagatatcg tattgctttt
    76981 cagcagacac gatgatcaga ttcctatttt ttttgagaag gaatatggtg ttgaagttca
    77041 tgtgtacgat gatctgcggg acgacaaaga gtatattcct tcgattaaac catatttatg
    77101 gtggaaatat ttagaagaag atcattcgcg tgaggacgac cgatatttct atatcgattc
    77161 ggatgtcatt ttcaataaaa gaattaattt gcgcaaattg ccttctaaag atgatgtttg
    77221 gtattgtagc gactgctgta gttatctaag tcttgattat attagaagct gtgaaaacgg
    77281 agaaaatatt ctaaaagata tggcaaacat tgtaaatgtt acagtagaat ctttggaaac
    77341 tataaacact aattcaggag gcgcacagtg ggttattaac cgtcctaaag cgaattattg
    77401 gaaaaaggtt tatctggatt ctaatcggct atatcgctac cttagagggc aaaaaacaaa
    77461 tatacaaatc tggacagccg agatgtgggc acagctttgg aacatgatgt atttcaatat
    77521 tggtcctaaa gttcacgagg aattagactt ttgttttgct actgatccaa tagaaaaagt
    77581 taaagaagta aaaatcttac acaatgctgg agtaacaaca aacgatgaag atttattttt
    77641 caaagggaga tacgtgactt ctacgccttt tgatgaagat ttatcatttg taaacaagaa
    77701 aaaatgctct tacgcatatg ttaaagcaat taaggcggtg gttagatgac gcctgaacaa
    77761 gtgactgaag aattgctaat agctgtgaag gataatattt acgttacctg gaacgaagaa
    77821 gatgagtcaa ttaaaaagat gatagctaaa aatgctgttt atcttcaaag taaagtgagt
    77881 acaacacttt ctttttctcc tgaaagctta gaatacggat tgctaatcga aagatgtaga
    77941 tacgactgga atcgtgcttt agatgagttt gaacaaaatt tcgctagtga gttattaggt
    78001 ttcattcaac attatgcgct acaagaatat attgcaggtg atgtgaatgg cgaataatcg
    78061 tagactcgaa gaaacattca acgatggttg gttaaagatt ttgacgcaaa ctaccaaaag
    78121 aaatgaacta ggaaaaaaga ttggtgtaga agatacagaa atcacttctt taaaatttag
    78181 aaatctttcc atgagagata gtgatataac agctatggat gcgatgggat cgaaattaac
    78241 taagaaagta aagactccat ttcatccaat cgccaagaaa tttaataaag atcaatattt
    78301 tatcgtaatc gatagtatgc gttacaacgt tatctatgcc gattacgata atttttatat
    78361 ctatttttat cttgaaagtg tgggtgaata tggtgattga taattctaaa gaaaaagaac
    78421 gtttaaataa gcaaatttct gctatcaaaa cttccttaga agagcatttt ggcctcaaac
    78481 tctttcaaga ctccgtaggc gaggatgagc tacctgatga ttttaattac ttcattctcg
    78541 aaacaggaga aatagaaatg atcactgagc caaaatatag cgtgggtcaa aatctatatc
    78601 taactttcta ttcagaaaat agagaagatt taacaggaga ctcactagat attatttcat
    78661 tgattcaaaa tcgttcgatt cgttttcaga gaatggatcc caatcattta aaactagaga
    78721 accaagatcg ctatatcgat caattggtat ttacgtttag acgattattg aagagtgatt
    78781 gtcatggcta aaaatagttg ggagctaaaa ataaatggac atgatgaact tcttgtgcgg
    78841 atggaacgct attcaagcga gagcgaacga ctgattaacg aagcattgaa atcaaaaggt
    78901 tcggctattg cagtggatag gattacagaa aaaattcctg tttctgaagc agatttaaga
    78961 agagggcacc aacacgcaaa aaatagtcgt ccacttaaga ctcaatacat taatttgggt
    79021 ttcatcatta gacctacaag aaaatttgag tatttaaaat atcctgattt ggggataggt
    79081 acttctaaaa gaaatcagcc ggacgaattt atgagaagag gattaggtct tgcacttgat
    79141 ccaattacag aacttctgat tcgtcaattc gataaattaa ataaataggg ggaacaacaa
    79201 tggctaaaac aaaaactgta gtaacaacgt tcgataacgt aagtatcaaa cgaattgctt
    79261 ttaattttaa gaacgcagga aatgcaatcg caacgaattg taacggacaa ttagatggcg
    79321 aaacagaaat gcaaacggtg gttaaaaaat gtggagcgac agaagtaaaa tcaaaatcta
    79381 aaccaatcaa tatgacggta acaattactg cacatgtacc gatggaagtt tatcgacgtt
    79441 tcaatgggtt gaaacaagat ggacgtatta aaccaggcat ttactcttac ggtcctgatt
    79501 ccgtaggcga agatttctca cttgctgcag agatcgtgga tgacttcgaa gaaaatagaa
    79561 agttagttgg tatgttagca tgcacttcga atacaggatt aacattctct attgaaaatg
    79621 gtgcggatga agtagctgcg ttagaactag aaacaaaagt tatgcaagat gaatttggta
    79681 aattttatca tgaagcaatt gttgcagaac ttgaagaaga cttaacagat caatggatga
    79741 cgaatctatc tgctgatgtg attaaaaaaa cttcagttgt gacaactacg gccactcaat
    79801 cacagtaaaa aaaacggagg tagcgaaatg aacgaagatt actcaaaaat tgaactaaac
    79861 gatggaacaa ttttgaattt agaacctaaa ctgaatatca agaaattatt gatgatcaat
    79921 agagatttta acacagacga gtttgcaaaa atgactgtgg gaaaaggatc catggatatt
    79981 tctgttattc aaggtgcaaa ggctgtgtat attgcttacc gccaagcgaa catgactgat
    80041 tatatttcat tcgatgaatt tatcgataaa tgggattttg atatggctac tgccagctat
    80101 atttatcaat tgatgatgtt caaacaagca cgcgatgctt atcaaaaaga atttgaaaaa
    80161 gcaaataagg aaaaaaagct tcaaaagtaa aaattccaaa gctcttagtt gaaacgtggg
    80221 tcgatgtcta ttcgatgttg accgacgttt tttctatgcc ttcagatttg gttttaagcg
    80281 atatctgttt agatgacatt ttgcaaatgg cttacaacaa gagtgcttat gaaggatgga
    80341 aaaactatgc aataaaccaa tcccagaaaa actaaagaaa ggaggtaaaa aatggctaaa
    80401 aagagaacag aagcagaagt aactttcata gctaacgatg acggattgaa atctacgtta
    80461 aaagaaatca gtgctgaatt aactaaaaat agagcagaat taaaactaga acaagctcaa
    80521 ttacaacaga ctggttctga atcagacaag ttaggaagta aattatcttc tttagaaaag
    80581 cagtatgaat tacaaagtca aaaagttgaa gtaactagcc aacgtttagc caatgcgaaa
    80641 aaatattatg gagaaaattc caccgaagtt aagaaacttg aaagagaact gattaatcaa
    80701 caaacagcgc aacaacgttt gtcaaacgaa attgataaaa cgagtaatgc actagctcaa
    80761 gcaaaaggcg aaatacagac gtacgagtct acaatgcaac agttggatag cgaacaaaaa
    80821 aatgttcaag ctagtgcttc tctgattgaa tccgaataca aaaaatggca agcaactgct
    80881 ggtcaatcag cttctgaagc cgagaaatta gcgaaagccc aagaatatgt ttctcaacaa
    80941 tctgaaaatg cggagaaaac gatagatatc ctaagacgac agttagaagc tacacagtct
    81001 gaatttggcg ctacatccac agaagcaatg cagatggaag cgaagcttaa tgatgctgaa
    81061 cgtgaatttg aagagttagg acaagctgct aaaaatgtag atacaactaa cttggacgat
    81121 atcggaagca aaatagatat gaataattta atggaagctt ctgacgtttt aagcgacatt
    81181 ggcgataagc ttacagaatt agggaaacaa gcagtggact ctgctaacag tgtaggtagt
    81241 tcccagagta agatacaagc taattttggt ttgtctaaac aagaggctga agaattaacg
    81301 aatgtagcca gagacattta ttataaaggt tttggagaat cgttagatca gtccacagat
    81361 gcattgattt tggtaaagcg taatttagga gatttaaata atcaagattt acaaaatatc
    81421 acggaacaag ctatggtcct agaaaacacc atgggcgctg atatggatga aacgttacgt
    81481 ggtgtaaatg gcttaatggt caatttcggc ttgagtgctc aagatgcaat ggatttaatg
    81541 gtttcgggta ctcaaaacgg tttagataaa acgcacgaat taggcgacaa tatggcagaa
    81601 tatagccaat tatggagtca aatgggatat tcagctgatg aaacgttcgg aatgcttcaa
    81661 aatggtttag atgcgggtgc ttataacctt gataaagtca atgacttagt taaggaaatg
    81721 ggaatatcgt taacagatgg tcgatttgag caaaacatgg atatgtttag tgaaagtact
    81781 agaaaagctt ttgaagagtg gaaaaatggc ggaggaacac aaaaagacgt tattaattcc
    81841 atgattcaag attttagcaa tatggatggt caatacgacc aattaaataa agcttcgaca
    81901 atttggtctg cgcttggcga agataatgcg atgaaagttg tccaatcttt aactgatgtt
    81961 aaccatacat ttgatgatgt tagtggatct gcacaaaaaa tgaatgaaga ttctactact
    82021 ccgttgcaag agttgaacgg gaaaatagct gaattaaagg attcattagc tcctataggc
    82081 aacacaatca tagatgcact cgaaccagta attgattttc taggaaagat ggctgatgcg
    82141 tttaataatc ttccacaacc agtacaggat ttcattgttg ctataggtgg tcttacagca
    82201 gcatttggca tactgttacc ggcaatacta gccgtgtcat atttatttgg tccgatgatg
    82261 cttataattg gaggaattat agctgttata gcaggcgtta ttgtggcaat taagaactgg
    82321 ggtgcaatta ctgactggtt tagtggttta tggaaaaaat ttactgattg gttgggtgat
    82381 acttgggaaa gtataaaaga cggagcctca tcagtttggg atggggttaa agaaacctgg
    82441 tctggatttg tagattgggt tcaagatatt tggcaaggag tttctgattg gtttggagag
    82501 ctatggagcg gattagttga aggagcttcc aacatctggc aaggagtcca agaaacttgg
    82561 caaacattcg ttgattgggt ttcaaatatt tggaacggag tcaaagaagt atggtcgatt
    82621 atttgggcag acattgtagg aattgttcaa ataccatgga cattaataac gtcattgatt
    82681 caagctggta ttaatattat cgtgggtatt tttgatgtag ctggacagtg attgggcgca
    82741 gcttggcaag ctgtttggac acctatttct gatttcctta aaaatacttg ggatactatg
    82801 acacaatgga taagtatcgc ttggaatggg attgtaacta cattccatac tatatttgat
    82861 ccagtagtgg catggtggaa tggtatatgg acagctatta gtactacggc ttcaaatatt
    82921 tggaattcaa ttagtgcaac agcttctagt atttggaaca gtatcaagaa tacaatcact
    82981 agcttggtac aagcagctgc tacagtaatt caaaatattt ggtcaactgt atctagttgg
    83041 ttaggtggaa tttggaattc aatcagctct acagcatcaa atatctggaa tagtgtgact
    83101 agtagtataa gcaatgctat aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt
    83161 atatcttcgt ggattagcgg aatttggaac ggtatcaaaa atactgcttt gaatctttgg
    83221 aatggaatta caagcactat tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc
    83281 ggttggtcca atctaacagg tattgtttcc gacatattca ataatgttaa aagtacaatt
    83341 gctaacatat gggaaggcat caaaaagact gttagcgctc caattgattg gattagagac
    83401 aaaatcagtg gcatttttga taatttgaat atttctatac cacatattcc gttaccggaa
    83461 tttatcatgg aaggcagctt taacccgcta aaaggtcaaa tcccccattt gcgtgtaaaa
    83521 tggcatgcta aaggaggtat ctttactaaa ccaactttac taggtggaat gaacggtgtc
    83581 ggtgaagcag gacctgaagc agttttacct ttgaaaagat ctgttttgca agaaattggt
    83641 gatcgtatct tgagtagcac atcagtttca tctagggcac aaacgattca acctgtgaat
    83701 aactacgaat tcaatttcac aattgatggt aacgcagatg aggttactat gaagcaaaca
    83761 actcaacaaa tcattgatag cattacaaaa attcaaaatg ataatgcttc ggcatggcgt
    83821 taaacaggag agtatttctc ctgttttttt agtattaaaa aggatgtgaa aaaatgactg
    83881 attgtataca ttctataatc gatggatttc ctgattattt gcataaattg gctttagctg
    83941 aaagaccaac cataccttct ccaaaaagac agagagttga aacttctgtt ttaggtaggt
    84001 taggtggctt agtacaagat tactcgtttg aagatatgtc gtttacattg cactataact
    84061 atttagagga tgtggaagac catcaagcgt tcaagcaatc gttttatatc atgcgtcatt
    84121 ggttaaacta tgcaaagaaa ttagaattct ctgacgatcc caacgtctat tacgttatcc
    84181 agactatcga tattggggat gcagaaaacg atattgttga atggggagag ttcgatgtaa
    84241 atattactgc gaaaccattc gcaagagttc aagaagatgt acctataacc gtagataaac
    84301 cacagtcatt taacttgctg aataatagtt tagaagaaag ttttccaaag attatcatca
    84361 ctccttcagc tacttcatgc cagttcatct taaatgatta tgtgtttagt ttcgaaggct
    84421 tagtagcagg aactgatgta gtcattgata gtgatttgat gctttgctac gaagagcaat
    84481 cggacggaga tattttagat cggtccaaca aaatgaagac catgcaatat ccgacattgc
    84541 aagtggatat caatcatttt aattgcactg gtttgagcaa aatacaaatt tatcgtaacg
    84601 ggttaaggta ggtgaaatag atgatcgata atttaataac tatttacgat aaaaatgacg
    84661 cgaataattt agctgaacat ttatatgata cgcaaggttt aggcgctttg tcagactggt
    84721 taacagctac tgttagcaat aaactaaacg gagccgagat atttcagggt acttattcaa
    84781 taaacggaac taatgcagat ttgattgtag acggacgtat tattcagtgt tatgtagatg
    84841 aaaatcgagc aaaacagcgt ctacggattt attatgcaaa aacttctgta ataggcaata
    84901 cgatagaagt aaaagctgaa cctattttca atgatataag aaaatcggtg ttgaataaat
    84961 atgacagcgg aacagaaaag atcactgcta ctcaggcatg gcaaaacgca aaagttttag
    85021 cgaaaccagc tatcccttcg cagttttctt tctcgtcatt agtagatacg cttgctaatg
    85081 tgaagataga aaaggcgaat tttttagaat tctttggtgg aaaagaggga tctattctag
    85141 atcgatttca tggtgagttt ctaaaagata ataacacatt acgtcatgaa aaaagtctag
    85201 gtacggatca taaaatcaaa gcgatttata ctaaaaactt aactggtctt gacttagaga
    85261 ttgatgctca aagtgtttta gttggagttt atccattcat tagcagctct tcagaaggag
    85321 aagacgagat cactctacca gaagaagtta ttttcacgga ttacgtggat gattatcctg
    85381 ctggatatgt ttcttttgtt gattttaaag acaaagcgac tgatgtagcc acattaaggg
    85441 aagctgctaa agactggttg aaaacaaaca tagataaaca aaaaccacaa gtgagtggtt
    85501 cgattgaatt agtaccattg aggcatcaaa gaggctatga aaaatttgtt gatctagaaa
    85561 aagtttcgat gggtgacgga gtagatgtgt atcatccaca gttaaaagtg aatatgtcag
    85621 cgagaattgt ggaatatacg tttaatgttc taaccaattc atacgataaa ttagttgtag
    85681 gaaacgtcaa aacaaacttc ttagaaaaca cagagaataa tgtcagcaat ttgattaatg
    85741 atgccattga tcaattgaaa aacggtggcg aaatcagtga tttactcaat gatattgtag
    85801 atcatcaaac tgatatgatt actggtcaaa atggtggtta tgttttatta gatcctaaag
    85861 aagcgcctag tcgtattttg attatggaca caccagataa gaataccgca aggaatgttt
    85921 tacaaatcaa caatgctggt attggttttt ctaaaactgg cattaatgga acatatgaaa
    85981 cggcatggac gttagatggc ggattcaatg cctcgtttat tacggctggt gaaatagtag
    86041 gaattactat tagaggtact acattaatta gtgatggtgc tgattataga acaagtattg
    86101 ctaatggcaa aatgacttgg tactcaaaaa aagttaacaa agatattatg gagctagaag
    86161 cacgtgatta tgtaagtgct gatgccggta ttgtatcata caccatgaaa actggtggtg
    86221 gtttcatgat tagaaatcca cagggtaact tggtttttag tacgtgggat aatggtaata
    86281 acagaccgtt tctatctttt ggtgcgccca atttcaggta tagcaatgct agttatgtaa
    86341 cttctggcga cggtagttct ttaagcatta atggtagtgc gggtaactca tgggaattta
    86401 aggtagctgg taggactatg aaatttacta gtgatggtat gctaacgtta ccaggttgtt
    86461 tttttggttc atgggaagat gggaaacttg ctaggtttga acaatcaacg gtacaagtat
    86521 ataaagattt tactgttaga ggtactaaaa actcaactgt accaacagaa cattatggac
    86581 aacgactatt gaacgcttat gaaactccag aatattattt cgctgattat ggggaagccg
    86641 ttacaggtga caatggtaaa gttcgtgttg atattgaccc catgtttgct gaaacagtaa
    86701 atctaagtcg gtatatgaca catgtgacac ctacagaact agttttgtgt gctgttactc
    86761 atgaagatat tgaccatttc atcattgaaa ctagtaagcc aaacgtatta gttagatgga
    86821 atttagtggc acaccgtcta gggtatgaag atattagatt aaaagaggat acagcatatg
    86881 atagcacagt gcttgaccaa aaacgttttt aaaacgaaga caaggaggta tataaatggc
    86941 tagcagttta tataatttgg ctttagattt cagcaaagaa ttaaactaca ccaaagctat
    87001 tatggctcgt caaggtgata aagggattac ggtgacggtt aaaccgtttc taaatggctt
    87061 gcagatggat acgagtggcg gaacatttac tttaaaagga acaacaccat ctaaccgtta
    87121 cgtagataat gttgcaacta gtgtaactag tgaagaagtc acgttttctc ttgatggcac
    87181 atttatgagt gaagcaggat attataaaca ctgctacgta gaatatagaa aagacaatca
    87241 aattttaaca acgcaagata tcattttttt ctcactagga gtgtctgaca tttcgcaagg
    87301 ccaagccgat gaatatgttt cgcaattaga agagttgatt cgaaagtaca acgaaacttt
    87361 tgatgctttt atggctgaaa tcaaaggtag agtggatagc ttaaatcaac agattactga
    87421 tttaactggt caagctaaaa cgctacaaga caagttagat gctctgaaag aagaaatttc
    87481 taagttaggt aacttacaag tgatgtacag taacagcatc gacttcgggg actatgatta
    87541 ttcggagaac ccgaatttaa tgccatatat tacagaacct tgggttggac ctttgttagg
    87601 taatggacat acagttaaag atagtgttaa aagggttata actcatacta aaactagaac
    87661 agctaattcc ggcgacatat tatcgcttgg cttaggtatt ccgtgtacag cggaagctaa
    87721 taataggtat cttataacca ctttacgacc atctacgaca tacacattaa gcgttactat
    87781 gagtgtcggt tcagattgga ctggagagac caatactata ggggttaggc ttagatattt
    87841 aaatgaacaa ggtggtatag aactgcccat taatgcatta ataccagcaa atgtagaacg
    87901 cgataaaatg gtcactcata catttactgg tataacaaaa gataatgtaa ctagcataac
    87961 aaactgttat gttgaaatat tctcacttaa tagtgaatat aaaggaacgg ttagtgtcag
    88021 ctatgacgtt aagttaaaag cacactaccc taacttacta gatggccctt actggttagg
    88081 taaagttcct ttaggcgaga atattgctga ccctacagtt gtctttccac acaaaactag
    88141 tgaatatatg gtttatggta gacgcaatac agaaaactat atcgctgacc aaacatatac
    88201 catatcaatg aaagcaacta aactaacagt tcaatcgttt gccgtgtata ttgctgctgg
    88261 ccgcgttaaa gtaggagaca tgaagcctac tgaggggtta gctaatacat gggagttaac
    88321 ttttactgta actaagcaac atatagatag tggggtaact aattatctgg aaatatacca
    88381 atacccgtca gctacaaaag gagctgttca aattgagtgg ctcaaactag aaaaaggcaa
    88441 tactcgaacc ccgaatatta gtgagtataa atatcgtggt actggtatgc gtgattcaaa
    88501 caatccaaaa gattatgttt gggatctagc accagaatat gtcgaagata atttggccac
    88561 agatattaaa atttctgaaa ttactggtaa agcaaacaat tataccgatg ggaaagtatc
    88621 ggagattaat tcgcagttga ctgcttcaat taatgaagta gacaccacag ctaaggatgc
    88681 tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa ttagacaata agatcgatga
    88741 acgcattaat gatacagcta ctaccacatt aacagttaca aacgggaata ccggatcagc
    88801 aaagctttat cgtgaaggaa aaacagtttc tatatatttt gtggctttaa acggaaaaag
    88861 cagtggtgga aatgattcaa cgatactaac aattccagaa ggctatcggc caccaattag
    88921 ttttgagcaa ctggttggct cgatagaccg ttctactttg aacagtgctc agttatctat
    88981 tggtgcagat ggagccatta aatggcgaag aaactcaagt tatggatcgg attatacctt
    89041 tgcaattact tacacgattt agaaaagcgt gaatcgatat gaaggcagca tatagaccaa
    89101 ttgaacctta cggattcgag caaatcattg tgaatgatga agaacattta ccggaagaat
    89161 gcacagaagt cgaaccaccg attccaaatt ggaaaccgaa attcaattac tgggagggaa
    89221 ataaatgaaa aacatttgga aatatggacg tactggcgga gagtacgcag gaaaagtatt
    89281 ggacgacatg cttgtatccg ttccttacac agatcagcca ccgcttgaag gaattcgtgc
    89341 tgatggcgaa ccgttaacaa tcgctgatca aatgtttgat cctaaattga atcaatggat
    89401 tattttagcg aacgcactag atcacaacga tttaaacaat ctcaaagcaa tgtatgagtc
    89461 gttagaaaat gagaacggcg atttaaaaca gatcaatgcc aaactcatgc taagcgatgt
    89521 agcaattaaa caggaaaata ctgcattgaa agaaaaagcg gatagtttag cacaaatcaa
    89581 ttcaaaaatg atgcttgctt cgttacaaaa tagcaaagac atttcagaaa ttaaagagca
    89641 actaaatcca gcttcaaagg gaggtgagta gtatgtttag ttttagcgat gtgaaaatga
    89701 tgtatgattg gggctgtttc actaacgaac aagtaatggt tttcgttccg ttgtgcatta
    89761 ctgaagaaaa agcagataaa atcattagca aagaagagag cgcatcttaa ttgatgtgct
    89821 ttttattttg attcaaggag ttgtcacatg attaatttag gggaatgggg agcgatagca
    89881 ggatcaataa ccgctatcgt ttctttgatt ttattagtaa taaaaccaat tactgcatct
    89941 ttctcgaaga ttactgagac tctttcaaaa gtaagtcaca atttagattt gctgactaaa
    90001 gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa gaaacacgat
    90061 gaaagattag atacacatgc agaaaaattg gtagaacaca cgcaacaaat taaaacttta
    90121 tttagggaga gaagaaaatg aataataaaa cgttcgaagt actaaaatgg ttcgcactgg
    90181 taattattcc cgcactagct actttcgtgg ggttagttgg taaagcgctc aattggcagt
    90241 acacagatat ctgtgttgtc atcattactg gttttggcgc gtttttaggg agtgtgttgg
    90301 gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag cgaagaagga ggaaacaaat
    90361 gaaaaagaaa attactatta ctgcgatgag cctgttaacg gctctttttt tattacccat
    90421 taatacgttt gcttatacta ttaatgacga gtataattta gcgccgaatc aaggagactc
    90481 cagattagca attcctaaca aaattatttt gcacgaaact ggaatagatg caccagcaag
    90541 aaacgtagcc gccaacatga aaaataatta taacggaagc aatccttata ctacagatgt
    90601 tattggtgac ggtgggattg tttaccgtgt gggtgagcaa ggatatgttt cgtggggagc
    90661 tggtaacgct aatccttatg cgcctgtaca gattgaatta cagcgcacat atgataaagc
    90721 attgtttgaa aaaaactatc gagcttatat tgaatataca agagatagtg caaaaaaata
    90781 tggaattcca ttgactcttg atcaaggaac ttctttattt acaaaaggaa tcatttctca
    90841 tttgtgggtg acaaattatg tttgggggaa ccacacagat ccatatggtt acttatcgca
    90901 aatgggagtt agcaaagaaa agcttgctta tgatttagct catggattta ccgatgaaaa
    90961 tccaacaact tctgaaaaca agcctgtcat tgatccaaca cgagctggtg cagctaatcc
    91021 tacgctgaca gatggaacaa atcacgccca cattgatcag tttggggaaa tcgaaaacgc
    91081 aaacttgcat gtggctggat ggcacattgc taactataaa tacgagtata ttttcattat
    91141 ggactacaat actgggaaag aattagctcg agtaagagct gatggaattt atagaccaga
    91201 tgtaaaccaa gcttataata cttctggaaa tgttggttat catgtatctt tcaatatgcg
    91261 taattttcct agtaagaaag tctatgtaat gatgcgggca acgaatgatc cagagggaaa
    91321 cactaaaggc ggtgcgcaag atttccatga caaacgttgg tatttaaata ttcctaaacg
    91381 ataaaaatag ctcctcgttg aggagcagta cataactata ttgacaacta taaaaattat
    91441 tcgataaaat agtgatgtta tcgcatatct tcactatcac ccataaatag tcacactcca
    91501 agctatgcga taacaggttt gttgccacac attctactgg ttgattgttt atggctttat
    91561 gtggcaacaa ccagtaccct tagctcagtt ggtcagagca gacggctcat aaccgtccgg
    91621 tcgtaggttc gagtcctaca gggtacatta acgtagccat ttgaatcgtt ctgtgttaga
    91681 atttttttga agagtattat acaagctaaa gcttttcttc attgccactc aaatgagtgg
    91741 cttttttatg tatcctttta tggattaatg aaaggatgtt tcacatagtt atacttctgt
    91801 atatttgaaa agttttactt tgatttttaa atagaaagac atttgggtta aattgtgaga
    91861 taataataaa gaagagttta aagcgcaccc caaaccactt ccccataagt gtgttacgct
    91921 ttaaactctt ttatatttga agccattaaa aagcatacca tatttttgaa aaaaagtgag
    91981 aaaaaaggct tacaattgga gtggtagtta attagtgact tatttttgat tttatagcac
    92041 tgatactata aaatatagat atcatcatat tacacaatct taatactaac ttaaaaaata
    92101 tctcctttca caagtatggt gataaaattc gttccgggct acctttttag gtagcctact
    92161 ttaatcttta tacctttctg gatcaacgaa agtatacttt atatagtcat aacgccgatg
    92221 atcgctacgt gcgtccggca cgtcagtc
//
LOCUS       DAZUMV010000002        65559 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000002 DAZUMV010000000
VERSION     DAZUMV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.002"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            217..849
                     /locus_tag="ACG8FK_000120"
     CDS             217..849
                     /locus_tag="ACG8FK_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5095762.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            972..1610
                     /locus_tag="ACG8FK_000121"
     CDS             972..1610
                     /locus_tag="ACG8FK_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS5095763.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(1729..1801)
                     /locus_tag="ACG8FK_000122"
     tRNA            complement(1729..1801)
                     /locus_tag="ACG8FK_000122"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1766..1768),aa:Thr,seq:cgt)
     gene            1922..2164
                     /locus_tag="ACG8FK_000123"
     CDS             1922..2164
                     /locus_tag="ACG8FK_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095764.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2303..2932)
                     /gene="upp"
                     /locus_tag="ACG8FK_000124"
     CDS             complement(2303..2932)
                     /gene="upp"
                     /locus_tag="ACG8FK_000124"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS5095765.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3066..4013)
                     /locus_tag="ACG8FK_000125"
     CDS             complement(3066..4013)
                     /locus_tag="ACG8FK_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS5095766.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4166..5410)
                     /gene="glyA"
                     /locus_tag="ACG8FK_000126"
     CDS             complement(4166..5410)
                     /gene="glyA"
                     /locus_tag="ACG8FK_000126"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS5095767.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(5585..6610)
                     /locus_tag="ACG8FK_000127"
     CDS             complement(5585..6610)
                     /locus_tag="ACG8FK_000127"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS5095768.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(6632..6811)
                     /locus_tag="ACG8FK_000128"
     CDS             complement(6632..6811)
                     /locus_tag="ACG8FK_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095769.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(6804..7643)
                     /gene="prmC"
                     /locus_tag="ACG8FK_000129"
     CDS             complement(6804..7643)
                     /gene="prmC"
                     /locus_tag="ACG8FK_000129"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS5095770.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(7636..8709)
                     /gene="prfA"
                     /locus_tag="ACG8FK_000130"
     CDS             complement(7636..8709)
                     /gene="prfA"
                     /locus_tag="ACG8FK_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS5095771.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(8712..9296)
                     /locus_tag="ACG8FK_000131"
     CDS             complement(8712..9296)
                     /locus_tag="ACG8FK_000131"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS5095772.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(9402..9650)
                     /locus_tag="ACG8FK_000132"
     CDS             complement(9402..9650)
                     /locus_tag="ACG8FK_000132"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5095773.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            complement(9641..9871)
                     /locus_tag="ACG8FK_000133"
     CDS             complement(9641..9871)
                     /locus_tag="ACG8FK_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095774.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            9977..10579
                     /locus_tag="ACG8FK_000134"
     CDS             9977..10579
                     /locus_tag="ACG8FK_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5095775.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(10683..11252)
                     /gene="lepB"
                     /locus_tag="ACG8FK_000135"
     CDS             complement(10683..11252)
                     /gene="lepB"
                     /locus_tag="ACG8FK_000135"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5095776.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(11482..12249)
                     /locus_tag="ACG8FK_000136"
     CDS             complement(11482..12249)
                     /locus_tag="ACG8FK_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS5095777.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12246..12725)
                     /locus_tag="ACG8FK_000137"
     CDS             complement(12246..12725)
                     /locus_tag="ACG8FK_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS5095778.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(12850..13587)
                     /locus_tag="ACG8FK_000138"
     CDS             complement(12850..13587)
                     /locus_tag="ACG8FK_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS5095779.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            13748..15148
                     /locus_tag="ACG8FK_000139"
     CDS             13748..15148
                     /locus_tag="ACG8FK_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5095780.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15371..16723
                     /locus_tag="ACG8FK_000140"
     CDS             15371..16723
                     /locus_tag="ACG8FK_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS5095781.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            16716..17414
                     /locus_tag="ACG8FK_000141"
     CDS             16716..17414
                     /locus_tag="ACG8FK_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS5095782.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(17476..18627)
                     /locus_tag="ACG8FK_000142"
     CDS             complement(17476..18627)
                     /locus_tag="ACG8FK_000142"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS5095783.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(18640..19077)
                     /locus_tag="ACG8FK_000143"
     CDS             complement(18640..19077)
                     /locus_tag="ACG8FK_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5095784.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19092..20858)
                     /locus_tag="ACG8FK_000144"
     CDS             complement(19092..20858)
                     /locus_tag="ACG8FK_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS5095785.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            21122..21799
                     /locus_tag="ACG8FK_000145"
     CDS             21122..21799
                     /locus_tag="ACG8FK_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5095786.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            21802..22929
                     /locus_tag="ACG8FK_000146"
     CDS             21802..22929
                     /locus_tag="ACG8FK_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5095787.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(23129..23569)
                     /locus_tag="ACG8FK_000147"
     CDS             complement(23129..23569)
                     /locus_tag="ACG8FK_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5095788.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(23580..25613)
                     /locus_tag="ACG8FK_000148"
     CDS             complement(23580..25613)
                     /locus_tag="ACG8FK_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS5095789.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(25615..27318)
                     /locus_tag="ACG8FK_000149"
     CDS             complement(25615..27318)
                     /locus_tag="ACG8FK_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5095790.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(27412..28362)
                     /gene="manA"
                     /locus_tag="ACG8FK_000150"
     CDS             complement(27412..28362)
                     /gene="manA"
                     /locus_tag="ACG8FK_000150"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS5095791.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(28401..29426)
                     /locus_tag="ACG8FK_000151"
     CDS             complement(28401..29426)
                     /locus_tag="ACG8FK_000151"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5095792.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHGG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(29635..30504)
                     /locus_tag="ACG8FK_000152"
     CDS             complement(29635..30504)
                     /locus_tag="ACG8FK_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5095793.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31208..33100)
                     /locus_tag="ACG8FK_000153"
     CDS             complement(31208..33100)
                     /locus_tag="ACG8FK_000153"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5095794.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33097..34824)
                     /locus_tag="ACG8FK_000154"
     CDS             complement(33097..34824)
                     /locus_tag="ACG8FK_000154"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5095795.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(34840..35490)
                     /locus_tag="ACG8FK_000155"
     CDS             complement(34840..35490)
                     /locus_tag="ACG8FK_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS5095796.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            35694..35990
                     /locus_tag="ACG8FK_000156"
     CDS             35694..35990
                     /locus_tag="ACG8FK_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5095797.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36182..36802
                     /locus_tag="ACG8FK_000157"
     CDS             36182..36802
                     /locus_tag="ACG8FK_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5095798.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(36894..38060)
                     /locus_tag="ACG8FK_000158"
     CDS             complement(36894..38060)
                     /locus_tag="ACG8FK_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5095799.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38072..38425)
                     /locus_tag="ACG8FK_000159"
     CDS             complement(38072..38425)
                     /locus_tag="ACG8FK_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5095800.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            38553..39575
                     /locus_tag="ACG8FK_000160"
     CDS             38553..39575
                     /locus_tag="ACG8FK_000160"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS5095801.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            39594..39755
                     /locus_tag="ACG8FK_000161"
     CDS             39594..39755
                     /locus_tag="ACG8FK_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095802.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(40014..40448)
                     /locus_tag="ACG8FK_000162"
     CDS             complement(40014..40448)
                     /locus_tag="ACG8FK_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095803.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(40558..42417)
                     /locus_tag="ACG8FK_000163"
     CDS             complement(40558..42417)
                     /locus_tag="ACG8FK_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5095804.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            42418..42558
                     /locus_tag="ACG8FK_000164"
     CDS             42418..42558
                     /locus_tag="ACG8FK_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS5095805.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(42535..43695)
                     /locus_tag="ACG8FK_000165"
     CDS             complement(42535..43695)
                     /locus_tag="ACG8FK_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5095806.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(44002..45426)
                     /gene="araA"
                     /locus_tag="ACG8FK_000166"
     CDS             complement(44002..45426)
                     /gene="araA"
                     /locus_tag="ACG8FK_000166"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS5095807.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(45442..46140)
                     /locus_tag="ACG8FK_000167"
     CDS             complement(45442..46140)
                     /locus_tag="ACG8FK_000167"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5095808.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46097..47758)
                     /locus_tag="ACG8FK_000168"
     CDS             complement(46097..47758)
                     /locus_tag="ACG8FK_000168"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS5095809.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            47976..49376
                     /locus_tag="ACG8FK_000169"
     CDS             47976..49376
                     /locus_tag="ACG8FK_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS5095810.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(49628..50707)
                     /locus_tag="ACG8FK_000170"
     CDS             complement(49628..50707)
                     /locus_tag="ACG8FK_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5095811.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(50793..51752)
                     /locus_tag="ACG8FK_000171"
     CDS             complement(50793..51752)
                     /locus_tag="ACG8FK_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS5095812.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(51764..53275)
                     /locus_tag="ACG8FK_000172"
     CDS             complement(51764..53275)
                     /locus_tag="ACG8FK_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS5095813.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(53297..53860)
                     /locus_tag="ACG8FK_000173"
     CDS             complement(53297..53860)
                     /locus_tag="ACG8FK_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5095814.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(53866..54729)
                     /locus_tag="ACG8FK_000174"
     CDS             complement(53866..54729)
                     /locus_tag="ACG8FK_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5095815.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(54743..55639)
                     /locus_tag="ACG8FK_000175"
     CDS             complement(54743..55639)
                     /locus_tag="ACG8FK_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5095816.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(55814..57103)
                     /locus_tag="ACG8FK_000176"
     CDS             complement(55814..57103)
                     /locus_tag="ACG8FK_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5095817.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57075..58592)
                     /locus_tag="ACG8FK_000177"
     CDS             complement(57075..58592)
                     /locus_tag="ACG8FK_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS5095818.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            58790..59680
                     /locus_tag="ACG8FK_000178"
     CDS             58790..59680
                     /locus_tag="ACG8FK_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS5095819.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(59753..60577)
                     /gene="nadE"
                     /locus_tag="ACG8FK_000179"
     CDS             complement(59753..60577)
                     /gene="nadE"
                     /locus_tag="ACG8FK_000179"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS5095820.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(60589..62061)
                     /locus_tag="ACG8FK_000180"
     CDS             complement(60589..62061)
                     /locus_tag="ACG8FK_000180"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS5095821.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(62698..63060)
                     /locus_tag="ACG8FK_000181"
     CDS             complement(62698..63060)
                     /locus_tag="ACG8FK_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5095822.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63283..64035
                     /locus_tag="ACG8FK_000182"
     CDS             63283..64035
                     /locus_tag="ACG8FK_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5095823.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64175..65098)
                     /locus_tag="ACG8FK_000183"
     CDS             complement(64175..65098)
                     /locus_tag="ACG8FK_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5095824.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(65113..65385)
                     /locus_tag="ACG8FK_000184"
     CDS             complement(65113..65385)
                     /locus_tag="ACG8FK_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095825.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        18961 a        13435 c        11411 g        21752 t
ORIGIN      
        1 ttaaaaacat tctaaattaa atcagtaaaa atttttattc taagattttt attagaatta
       61 cgcctaattt gtgtctctca gggcaattct aagaacgttt caagagatag cgacactatc
      121 gttatcgaaa aaggcgaaaa cctatctcat tttctgctaa tgtaagttgg tttagctttt
      181 gttaatatga ctttatgaaa atggaggtaa ttaaatatga tttttcctaa agttaatgaa
      241 gcaagcttga ataaaggatc cttttttcat atctatgaaa acgaagattt tgaactgtat
      301 aatatccttt cacaaaaaga ggtcatcgaa aaccaaataa tctgccatcc aattatttac
      361 aaatctattt tcaagtatgc attttttgct tccaatgatt ttcaaagaat ggagattacc
      421 gactgtatat tcgaaaactg tgatttttca ggctgtatat tgacaaaaag catgttgcat
      481 cgcgtgaaat ttgtcaattg taaattaaca ggtactaaat tgatggaaag ttatattggg
      541 aacacgttat tcgagaattg taaaatggac tatgtaaatt tatctggttc aaacataaaa
      601 gaaagtacct ttgagcactc tgtcttatca agcgcagatt ttgtagactg ttccctcact
      661 aaaataatgt tttctactaa tgatttagaa aatgctagct tttttgatac tgatttaaaa
      721 aatatcgatt taagtagcaa ctcctttgaa atgattgaag tacgctcgga acatctaaaa
      781 aattgtacgc ttaatcagtt gcaagcactt ggttttgcta gaaaattttt acaaataaaa
      841 gtagtataat caaaatgaat atataagata aatttttagc aatacaaata tccataaaaa
      901 aacattgcct attttaactc ttcatgtata ctgactctat cttcaaaaac tgaatagtag
      961 gagttattat catgaataac attttaggaa aaaaaataga acttaaaaaa gggatattgg
     1021 gcactattgt cgttgtactc ggtttgttag ggttgctatt tgcgccatcc attattagca
     1081 ttttttcttt aactatcact ggatttatag catttacact tgtttttggt aaaaaagata
     1141 cgaaaaaaat gttctcgaaa cctgtcgctc ctgtcaaaaa tatagccaaa tattttttta
     1201 tcaatgtagt aatcagtgcc gcagtctcgg tatttttgca acaaattttg aaatggggtt
     1261 taacaggcaa tccaataaat gaggctttca gcccgttatt atttattatt ttgcctatca
     1321 tgatacttgg agaagaattg ttctcagtgt attttctcgc tattttttct agtaagttca
     1381 gcattcctgt cgctagtttc ttgtcagcaa tcatctttgg atttatccat tacagtactt
     1441 atgataatgg aaatatttta cacactgtag cacacatcct tttgatacaa ggggtggcaa
     1501 gattgttatt caaccaagct gccattaaaa gcaactcgat cattacaagt tgggcagttc
     1561 atgtgatatt tgatttcaca gctatattgc tagtcgtttt attctcctaa atttttatag
     1621 aaataataca tgctctattg ctaagaaagc atactttatt tcctttaaac aaaaagacag
     1681 atgtatcaat ggattaaaaa tccttgatat atctgtcttc tcttttaatg ccagctatag
     1741 ggatcgaacc tacgacctac gcgttacgag tgcgttgctc taccaactga gctaagctgg
     1801 ccttatccaa aagaaattat atactctatt tggctgattg taaagagtcc gatggaaaat
     1861 tagtttaatt tcatgatatt tttcataaaa cgtggtataa ttgatgtgaa aggcaggttt
     1921 tatgaaaatg gcaacttttg gaaaatttga tacaactatc gtaccatcgg aaattggtga
     1981 aaaattcaag atcaatactc acgtaatgtt cacaattcac ggcaagtcgc aaactggaac
     2041 gatttcaaag caattgaaaa attccgctgt tgttgagatt gatgaaactg cagaaaatgc
     2101 gaacttgatg acgcaaagta agggtgtagt aatcgttaac tacaaagaac taaaaaagat
     2161 ttgaatttga ccatctctgc atttttggga tggttttttt atatgcaaac gaaagctgtg
     2221 aaaaagctgt tctgcatcaa acgttaaaaa tgaccaaaaa aggatgtgac aaaagttgtg
     2281 tcacaccctt tttcgtattt acttattttg taccaaacaa acggtcaccc gcatccccta
     2341 gtccaggaac gatataacca tgttcattca atttttcatc tagagaagct gtatagatgt
     2401 caatatcagg atgcgcttct tgcaaagctt ttactccctc aggtgctgct accaaacaaa
     2461 caaatttaat gttcgacgcg ccacgttctt tcaatgcatc gattgccatg attgctgatc
     2521 cgcctgttgc caacattgga tctactacaa acaactgacg tgcatcaatg tcttctggaa
     2581 gtttgacgaa atattcatgt ggttccaatg tttcttcatc tcgataaaga ccgatatgtc
     2641 ccactttagc agctggaatc aattccaaga taccgtcaac cattccaata cctgcgcgca
     2701 agattggaat aatcgctact tttttaccag acaacgtttt ttgtgttgtt tcagtgatcg
     2761 gtgtttcgat cacgatgtct tccaatggca tatcccgtga tacttcatat gccatcaaca
     2821 tagcgatttc atcgactact tcacgaaaaa cctttgttcc gcagtttttg tcccgaataa
     2881 tagttagttt gtgttgaatc aatggatgat cgatcacttg aaatttgccc attttttaag
     2941 ctctcctttg ttctgtcgtt tctttttaat tgtaaaacaa aatcaagtga ttgactagtc
     3001 aaactcgcca tttttcctaa gaaaatccgt ttttgaaaag aagttttcct gcggacaagc
     3061 aaagcttatt cttttttctt gatccaagaa gtagataatg ccattgctgc acgcgttgcc
     3121 tttgtctgat ccatagcatt taccatgaca aaatcatgga tgattccttg gaagcgtact
     3181 tgtgtaacag gaacccctgc atcacgaagt ttgcgcgcat aagcctcacc ttcatctctt
     3241 aatacatccg cttctcccgt taaaatcatt gctgaaggaa gatcatttag ttcatttata
     3301 tttgctctca aaggcgaagc tgtgatttca gcacgctctt cttcattcgt cgtatattgg
     3361 tcccaaaacc acatcattcc ttctcttgtc aaaaaatagt tttctccgaa ttcatgatag
     3421 gaatctgtat caaaatcagc gtttgtgact ggataataca gcaattgctt ggatattggg
     3481 aggccctttc gttcttttgt taagatcgtc atgactgtag ccatgttacc acctactgaa
     3541 tctcctgcaa cgattagatt ctcgagatcc agattttttg acttggctaa tttattcagc
     3601 tgttgcaaca cagcataatt ttgttcaatt gctgttggat attttgcttc tggtgatcga
     3661 ctatattctg gaaagacaac gacggaattc gtacgaaccg caagttctct gatcaattta
     3721 tcatgggttt gagcactgcc aaaaacccag cctgctccat gaatataaaa aataactggt
     3781 aatttatcca tattcccttc tggtcgtacg agacgaatat tgagttttcc ccattcgcca
     3841 gtatccacca tcaagtcttc aatatcgacg gaatatttgt tcacaggtga ttcttgtact
     3901 tcttctagta aaactcttcc ttcttccggt gctagttcat agatacgcgg atgaggcgca
     3961 tttgcatcac tgaatgctac tgcttccttt tctaacgaga tacgatcagt catgactttt
     4021 cctccctgag atttagtgtg aagactaaaa ttacacctca tatactagag agcatcaccg
     4081 atattgtcaa agaatccgtt ttcccaaaaa ataaacagaa aagcccaaca ttgaactttt
     4141 ctgtttacta atggaattta aatttttatt ttttatgcaa cggaaatttt tcagttaatt
     4201 cgcgtacacc tgttttgact tcgtctagtt gagcgttatc atcttttgct tgtaatgctt
     4261 tgacaactag ttcagccact tttacagcat cttcttcttt gaacccgcga gtagtgattg
     4321 ctggcgtgcc gattcgaata ccgcttgttt tgaatggact tagtgtttca aaaggaatcg
     4381 agtttttatt tacagtgata ttcacactgt ccaaaatgct ctctgcttct tttccattta
     4441 tacctaactc gcgaacatcg atcaacatca gatgattatc tgtcgcccct gaaatgactc
     4501 tggttccaat tgcttgattg aaaactttga ccattgcttt tgcattggcg atgatttgtt
     4561 cactatattc tttaaatgca ggatctaatg cttctttgaa tgctactgct ttacccgcaa
     4621 tcacatgctc tagagggccg ccttgaatac caggaaatac cgcactattt attttttttg
     4681 ctaatgcttc atcgtttgtc aaaatcatgc cgcctcgagg gccacgcaat gttttatgag
     4741 tggtagtcgt tgtgatatct gcataaggaa ctggattagg atgaagtcct gctgctacta
     4801 atcctgcgat atgtgccata tctaccatca gttttgcgcc tacttcatct gcgatttctc
     4861 tgaatttcgc aaagtcgatc gttcgtccgt aagcactggc acctgctacg atcaattttg
     4921 gttgatgttt tctagctaaa atacgtacca cattataatc aataacttct gttgtcggat
     4981 caacaccgta agcaacaaag tgataggttt tgccactgaa gttgactggt gatccatgtg
     5041 ttagatgtcc gcctgcggaa agatccattc ctaagatcgt gtcccctggt tctaccaaag
     5101 cgagataagc agccgtgttg gcttgagagc cggagtgagg ctggacgttc gcaaatttcg
     5161 ctccgaataa ttcttttgca cggtcaatcg ctaaattttc aacgatatcg acaaattcac
     5221 acccaccata atagcgatgc cctggataac cttctgcgta tttatttgtc aagatacttc
     5281 cttgtgcagc catcactgct tcggagacaa agttttcaga ggcaatcagc tccaaattat
     5341 gttcttgtct ttcttcttct ttggcgatcg cagcccaaag atcagggtca aacgttttgt
     5401 aatctaccac aaacaacagc tcctttttat tttcctacaa ttttattttt cccatcaaaa
     5461 acaaattgcc aaatattcgt atctcttcta ttacgattag ttacgcttag tttttcgttc
     5521 actgatagaa gatttacttg atagcattgt atcatactaa aagaatgcga caataaccaa
     5581 ttgatcattt ttcaaaataa ttttgtcctg ctgctttttt caagcgattc atgtaagcat
     5641 tccccaatcc agtttctgga tacacttgta caaaaatcaa atctaatcca agtgttggtt
     5701 catctaatcc tcttagacct gcaaacaatc ctttagccgc cgcttctact gagttgtcgt
     5761 tatacatata aacagctgca gtatctgtcc gcacttgatc cgcaatttcg gggcttgcta
     5821 tgacacctgc acggatcttc ttattttttg cccattgaac tgcagttgac caatcgccat
     5881 ctctgaccat cagaacacgt gtatcgggtg agtagtgttt gtatttcatc ccaggggctt
     5941 ttggtgtttc attttctttt acaagatgct gatcaatagc aacaggagat tcgatgactg
     6001 cttcgatttg ttctttcgtt actgcacctg gtcgtaaaat cataggcatg gctgttggat
     6061 cacttaaatc aagaacagtg gattctacac caattcgcgt agctccatca tcaataatcc
     6121 cggtaatttt tccttgtaga tcatgatata catgatctgc agtcgttgga cttggttttc
     6181 ccgacgtatt tgcacttgga ccgacaagcg gaacaccgga aagttcgatt acttctagtg
     6241 tttttttgtt atccggtata cgaaatgcag ctgtggataa tcctcccgtc acaactgagg
     6301 gcaaagtgtc ttttttgatc ttaaatatca aagttaatgg tccaggccag aagttcttca
     6361 caatcttctc tgctgccgga tgaaagttgt ccacatactc ttttacttgt tcaaaagagg
     6421 cgacatggac gattaatgga ttatcttgcg gtctcccttt tacagaaaac actttcttga
     6481 cagcgtttgg taaaagagcg ttggcgccca atccgtatac tgtttcggta ggaaaagcaa
     6541 caagttctcc ttctttcagt gcttcggctg cctcttgcaa ttctgtttca tcatatcgtt
     6601 tcgtttccac agtttatcca caaccccttt ttcatacatt cttattttgt ctgagtttat
     6661 cctcaagttt tccactgttt gtggataatc tatttgtcaa tagtggataa acttgttgat
     6721 aaaccaaaaa gtcgtttaca tccttgtcaa ataagtgttt ttttataata aaaaaacaaa
     6781 taaaaaattt ttcatccaca acttcaacca taaacttgga tcattcgttc attgccaaaa
     6841 agatcctttt ttatcgccac ttttttatct ggaaatgctt gctgaaatat tttttttact
     6901 gcttcacctt ggcgaaatcc gatttctaaa aaaatctttc catttgattt tagtacgctt
     6961 ggtgcttcaa ctgctatttt ttcataaata gctaatccat cgttctcagc aaaaagggcc
     7021 attttaggtt cgaatgaccg gacactttca tccatcagtt cccattcatt ccgactgatg
     7081 tacggcggat tagaaataat cacatcaaat tgctgatcca ttactggtcc taatgtatct
     7141 ccatgataaa aactgatttt cgttccttct ttctgagcat tttctttcgc tacttctaat
     7201 gcttcttctg aaagatccac agcagatata tgccaatttt ttcttgctgc tttcaaacta
     7261 atagcaattg ctccagtccc tgtcccaata tctatcactt ctaacggtac accaggcgtc
     7321 tcgtctaaac accattcgac taactcctct gtttctggtc gagggatcag tgttgcttca
     7381 gttaccttta accgatgatc ataaaaatct gcatatccta gcaaatactg aggtggatga
     7441 tctgcaagca gaagtcgcag atcggtttcg atcaaccgct gttcttcttc tgaaatctct
     7501 tcgttcatat gaaggagcca atcaagtttt ttccattgtt ttcgttccaa aaataaaaat
     7561 tggatactat atgcttcttt cccatgatcc tctaaaaaag aagaagcccg tgacaggact
     7621 tctcgatagg tcattttacc cattttgcat agcttccagt tttgatgttt gatcatacat
     7681 gatcaatgca tcaacgatct catctaattt ccctgctaaa atctgatcta atttttggat
     7741 agttaatccg atacgatgat cagtcacacg attttgcggg aaattatacg tccggatccg
     7801 ttctgaacga tccccagttc cgactgctga ttttcgattg gcatcatact cgctttgtgc
     7861 ttcttgctgg atcttatcgt acacacgagc acgcaagatt ttcattgctt tttcacggtt
     7921 cttgatttga gaacgttcat cttgcatagc aacaacgatc cctgttggta agtgagtcaa
     7981 acgtacagca gatgctgttt tattgacgtg ctgtccgcca gcacctgaag catgatagat
     8041 atctgtacgg atatctttgt cttcaatatc gatctctact tcttcagctt ctggcatcac
     8101 gacaactgtt gcggtggaag tatggatacg cccttgcgat tcagttgaag gaacacgctg
     8161 gacgcggtgc gcaccacttt catattttaa tttcgaatat acgttgtctc cggtgatcat
     8221 catgatcact tctttatatc caccgattcc ggtaacgtta gcttccatca cttctgtttt
     8281 ccagccttgt gcttcagcat acttttggta catattgaaa agatcgccag cgaataatgc
     8341 tgcttcgtct ccgcctgctg ctccacggat ttccatgatg atatttttat cgtcatttgg
     8401 atcttttggt aaaagcaaga tcttgatttt ttcttctaag acttcttttt ctttttttaa
     8461 ttcagataat tcttcttttg ctagttcagc catttcatca tccagttttt caccaagcag
     8521 ttcttctgta tcggtgattc cttgtactac ttctttataa cgacgataaa cttcgactgt
     8581 ttcgcgcgtg tttgcttctt cttttgaaag ctgcataaaa cgctttgtat cagaaatcac
     8641 ttctgggtca cttaaaagtt ctccaagctc ttcataacgg tcttcaattg cttgtaattg
     8701 atcgtacatg cttattctcc ttctgtaatc attgatgggt gaaagtaatg ttttcgacaa
     8761 acggggtaat aagcttcatt tccgccaatt tgcacttgat cgccttcata aacagggttt
     8821 ccatcaatat aatgtaaatt catgattgct tttttgtggc agaaccaaca gattgttttc
     8881 atttcttcga ttttgtctgc ataaagcaat aaatattttg atccttcaaa taactcgttt
     8941 cgaaaatcat ttttcaaacc aaatgccata accggaatat ccaattcgtc cacaattctc
     9001 gtaaaagcaa tgacatgttt cttttttaag aattgcgctt catcgatcag cacacaatag
     9061 ggtttgcttt ccattttctg aattacgtca aatacgtctg tttcttcaaa tatcggcatc
     9121 gcattacgtt tcagtccgat tcggcttgaa acaacgccta aaccatctct tgtatcaata
     9181 ccgcttgtca ttaagacaac tggcttattt tgttcttcat aattatgtgc gacttttagg
     9241 atctcgatcg tcttgccgct attcattgcg ccatacttaa aaaatagttg tgccatgctc
     9301 tttttcacct tccagtttgt tcctatggta ttatacttga ttttcagcat tttttacagt
     9361 gagaattttc atcaagcaca gattagtatt aaatctattg ctcacttttg ttccaatttt
     9421 ttgacaagaa ctaaacatgg gttccattca tcccataaat ttggaaaaac ttccaacctt
     9481 gaaaatccta atgattcgta gaaacaaata gtttgatcat atatagagta gtgcccctcg
     9541 tctacagtct tcacttgcaa atatttgtaa tgttttgctg cgtaaccttc tgcttcttta
     9601 aaaagttttg ttcctaatcc ttggtgatga tagtcttttt ttattcccat acaatagaat
     9661 agatttcggc agtatcttca cttgtctgtg aaagagtgat aaagcctaaa tattcttctt
     9721 ttcttttttc acaccataaa ggtagtttac ttgattcctc gatatatttt tccgttgatt
     9781 caggaagccc aaaccactca ggcaagtcta gtaatatttc tcggacacac tttccttttt
     9841 cctctctatc ttttacaaac gatatcggca tattctttct agattctccc tttttgaatt
     9901 gagtatagca tgctttcttt cttttcacgt gctataataa gttaaaattt caaagtttta
     9961 cgtaaggagc tatttgatga caaaaaatat ttttgattcc atcgctaacc attatgattc
    10021 ccctgaccgt caagcattag ccaagattat tcgcgaagaa ctaactactt atctaccgcg
    10081 cgacagccac caaaaagtcc tgctggatta cggaggcgga actgggcttg ttagtttacc
    10141 gcttgcggaa agattcaaag aactgatcat tgccgatgct tcagaaacta tgctgaaaat
    10201 ggcagaagaa aagatccaag cagcagatct gaaaaatgtc cgaacgatcc acgctgatgc
    10261 ttctgtcgaa tttcctgctg tccaagctaa tctcatcctt ctatcacttg tacttttaca
    10321 cattcctgat acggagaata ttcttacaaa actttatgaa attttagctc ctggcggtca
    10381 attgatcatc gtagactttg acaagaatga acaaattagt catccgaaag tccataatgg
    10441 ctttagccaa gaagaattga ataatcgatt ggaaaaaacg ggctttgtct ctgctactag
    10501 tcataccttc catcgtgggg aaaagctatt tatgaacaaa aacgcttcgc ttttcttatc
    10561 catcagccaa aaagcttaaa atgagattgc aaaaaaagtg agcaactcca agaaataaga
    10621 aggaattttc ggaaaaagaa gctgtgacaa aaaacatgtc acagcttctt tttatttttt
    10681 catcacatga ttttaatttc tgaaagaggc caataacgga aaacaacttt tccttggatc
    10741 atatctttgt cgactaaccc aaacattcgg ctgtcttttg aaatcaagcg gttatcgcct
    10801 agaacaagat actttccttt tggcacagta tattcaaagt cactcgtaaa ggtggatgaa
    10861 ctttctgctt gtgcttggaa tcctgagcta taagcatatt ctccttgtaa cttatcttct
    10921 gaaaattctt ttttgaactc atccagataa ggttcatcat actttttgcc attaatggtc
    10981 aactggtcat tttccatctt gactgtatct ccaggcatac cgatgatccg tttaacaatc
    11041 atccgttctt catcccccgg ttcctttgta gtaataatat ccattcgttc atagtttgag
    11101 atcttagatg aaatcaatcg ttgtccatca cttaatgtag ggtccatcga atgcccgttt
    11161 acagtaactg gtgtaaacac gtacactcta gctaataaaa tagccgctaa tacaaggatc
    11221 aaaatccccc aatttttcaa aaattctttc acagcttacc tcctacttgt attttcctcg
    11281 ttatttatta cgttttttca caatacttcg ttgtactttc tttattctac aatgtttata
    11341 aatgaaagga aagaataagt gatgtgttcg tcttatcttt ctcgttatat aaaaaaggaa
    11401 cgtgacaaaa gacgtgtcac atccccctta ttccgaataa acgtttttat cgcaaccact
    11461 gatacacggc gtttcatcac attatgaatg tcttcttctt tggctacgac tataaaccac
    11521 tggttcttcc tgcgactgtg ccttttttgc aatcaatttc agacgataag gctcatgata
    11581 gcgtaacccg tctttgacaa aatcaccgcc gacgatgaac aatccgacag gttccaatgt
    11641 gtcttctgtt gagtcaagtc gaatggtccc tactttttcc atgtccgagt agcttcgtcc
    11701 aatatttaga acatactctt tctctcctgt tttcttgaca tagtaataag gatacaatgt
    11761 atttctatcg accagaaatc catccatgcg accgagttca tttgttttga tctcaccatt
    11821 tccaatcaaa taccgcatat tctcggctaa tttcaattga taatcttcgt cataagaaac
    11881 catgacctct tgtgtattca ctttatcgat cgaatttcca ttatctatcg attcttctgt
    11941 ctggatgttg aacgcttcat caggtaaata cttcacaaaa agctgagtga catcggcttt
    12001 cgtgttttta tctaaaaagt aataaaatac atttaaaaca gtaaaataaa caagcactag
    12061 aaatgcaagt ccatataaga aaaattgaag atagttttgg ttttgccaaa gtccataaac
    12121 aattctcaat aaataaagcg tgggaatcac acttaaaatc gtatatccac ggttaagata
    12181 tttaggactg atgtctaaat agccaaggaa tttatgcacc ccgttgataa ggtctagtaa
    12241 aaatgtcatt gagaatcacc tgcttgctgg ttatacgttt gggtatttcc tgaatcttgg
    12301 ttttgctgtg ctgtacgtgc tgcatcatca ttttgcgtgt tatcatctac agtcgtactg
    12361 cttggtatcg tttcggagct agcacttgta tccggacttg tacttggatt ggttgaatcc
    12421 gatggagtgc catctggtac cgtattactt gtttcgcttg agctgtcagt tgtatatgtc
    12481 tcatcatacg acgaactagt tgttgtatcg tcccatgtac ttgtcgtagt ggtttcattc
    12541 aaactttccg ttgttgttgg ttcagatgtc gagactgaag tagttggtgt tgtttcttct
    12601 acagtactag aacttgaact tggtgcacgg tagctggaac tattcacaat acctgtagta
    12661 gtcgcgacaa tcgtcgagat tgttaataca gcgattggct tcaaaatagg tgctactttt
    12721 cgcatcttat ctcttccttc attgaaattt attcttgttt ccttacttta ctacggacaa
    12781 attatacagt ctaccttatt tctaccataa attctttatt tttttcttac gtttttccga
    12841 aagatttctt taattttcct caaaaactac ggttgatgtt tttaagtaag cttttgcttc
    12901 ccgattgatt tctttcagat cgcatccttg ctgcttggca gcgaacacgc aaccacaata
    12961 acattggcga taaatatcat actctttgca catttgcaaa gagcgttcat agcctttgtt
    13021 tttcttgaaa tcacttggca agtattgtgt cccgtaaatc ttttggatat ccattccgat
    13081 ctgattgatc aaagctgcat ttttctttgg cgagatggtc agggcacttc caaagtaatc
    13141 gtatcctagt tcttgcgctt tttgcgcaac aagatctaat cgcatattga agcatgcact
    13201 gcagcgagca ccaccttctt tttcgttttc aagatggttt gttaaaacca ttttggtgaa
    13261 ttcatttgga cggtattcat caacgattag cgcaacatga tggtcagttt tctcattaaa
    13321 ttggtctaca aatcgttttt gttcatgtaa tcttcgcaaa tactcacttt taggatgaat
    13381 attcgaattt gaaaaaaata acgtgatctc tgcatattga gacatgaatt ccaatgtgtg
    13441 cgtactgcaa ggagcacagc atgtatgtat taatatctta ggctttactt cttctttctc
    13501 ccattgcatg atcatttttt tcagaactgt atcataattt attttttgat ttttcatttt
    13561 ttctacgatt tgttgagcat taatcaattt gttattcctc ctccttcttt tttctaggat
    13621 tttccttatt ataagccatt tctattttaa tcgaaagctg tattgttatc tgtttgataa
    13681 tttctttgag tattcctaat tccccttgta tactggtaga aacgactatt gtcaggagga
    13741 tagtactgtg catgttgatt tattctttcg aaggctgcag cggcgaggac agaagtttgc
    13801 tgccaatcat ttctcatcga tccaaatcat cgttttttat tatatattga tgacggtctt
    13861 atcactcgtt ctattttata tgccgatttt tcgcgaacca gatagccatg tttcttttgt
    13921 ggacatgctg ttcatggcaa tcagtacagt gagtgtgact ggactatcga cttttgatat
    13981 caattctgtg ttcaatgata acggtattat actgctggaa atattatttc aagttggtgg
    14041 tttggggatc atgatgattt ctactgcttt tgtcattttt tctaaacgac ggatcacact
    14101 gaaacagcga cagctgatca tgacggatat gaatcaaccg cgtttgtctg gtatcgtccg
    14161 actgattcgg atcacttttg ctattttaat ctggtttcaa ttactttttg ggacattctt
    14221 ttctatttat ttttattaca gaggctattt tgatcgttgg cgtgatgccg ttttttacgg
    14281 tttttatcag gcgatatcgg ctgtcacgaa ctctgggttt gacgtcactg gggattccat
    14341 caagcccttt gcacatgact attttttctt gatcctgatc atgtttttga tttttgtcgg
    14401 aggaattggt tttcctgtat tgatggagtg ccgggaatgg ctattataca aacgttccaa
    14461 tgcgaagctt ccgtttcgtt tttccttgtt taccaaatta gctgtgttag cttttgttat
    14521 cttgtttgtt tcgggaactg tcttgattta tctattggaa aaggaccatt tattccaaga
    14581 ttccaatatg agtgtcaaat ggatcaattc gatgttttat tcgattacga ctcgtaatgc
    14641 tggtctgcag attcacgatt tgggtgattt tcaaatcaca acactgatca ttttttcttt
    14701 actgatgttt atcggatgca gtcctagttc tgtaggcggc gggattcgaa caactactgt
    14761 agcaatcatc ggtttgtatc tatattcctt cctcaaaagt gaagataaca tcaatatttt
    14821 tggacggaga atcgatcaag atgatgtgcg aaaatcagtc gttgtcttta tgctttctct
    14881 tggtatgtgt ttcttttgta tcgttttttt atctgctaca gaagaacaaa cgctgatctc
    14941 gatcatcatc gaagtaactt ctgcgtttgg aacaactggt ttatctttag ggattaccgg
    15001 agacttgtct gttgtgggca aaatcacgat tgctacactg atgtttattg ggcggatcgg
    15061 aatgctatat acattgatgt tattcgtgcc aaaagaaaca cgagatctag gctatgaata
    15121 tccaagcgag aaaattatta tcggttaaaa tgggaatata ggtaagaggg aataataaaa
    15181 tcacagcagc ttgaaacaat cattttgttt caagctgttt ttttgttaaa tcatgcgatt
    15241 ttgtccgatt tcatgcactt tcagccgtaa ccttttctta agaataagaa aagcaattta
    15301 accttttttt caacatatgg tatgattagt tgaatttatt cgtcagcaaa tcgtttttgg
    15361 aggttacata atgggaatac gcagtcattt tgcgattgca gttggaaaaa cgtcgcaatg
    15421 ggcattaaaa acttttttta aaggcggatc aagtttacca ggtaaactag cgctgaaagt
    15481 cgatccacat atactagata gtttggcaaa agactaccaa gtcgtcgttg tcaccggaac
    15541 aaacggtaaa acattgacta ccgctttaac agtcaatatt ttgcgccagc aattcgatga
    15601 agttctaacc aaccctactg gtgcgaatat ggtacaggga attgtatcga ctttcttgca
    15661 ggcaaaacca aaaaaaggac agaaaaagtt tgctgtccta gaaatagatg aagcaagttt
    15721 gagcaaagtc acagaatata tgaaaccaga actgttctta tttacaaata ttttccgtga
    15781 tcaaatggat cggtatggag aaatctatac gacctataaa atgattgtag acggtgccgc
    15841 aaagtcacca aatgcaacga ttatcagtaa cggtgattca ccaatcttca attctatcga
    15901 aacagtcaat cctagaaaat attatggctt tgaccatgaa gaagatcgtg aacaaatggc
    15961 tcattacaat acagacggtg tcttatgtcc aaattgtcat cacatcttgc attataaaat
    16021 gatcacttat gcaaacttag ggaaatatta ctgtccaaat tgtgacttca aacgtcctga
    16081 attggattat cgattaactg atatggtacg gatggacaat gtgtcagcag atttcgtgat
    16141 cgatggtcat gaatacggaa tcgaagttgg cggtatgtat aatgtctaca atgctttagc
    16201 agctactgca gtggcagagt acttcaatgt cgatcctgat aaaatcaaac aaggtttagg
    16261 atatgacgag aaagtattcg gccgacaaga agtcatcaat atcaatggga aaaaatgtac
    16321 attagtactt gtcaaaaatc ctgtaggtat caatcaagta atcgatatga ttggtctagc
    16381 tccttatcca ttttctttag tggctttatt aaatgccaat tacgcagacg gaatcgatgt
    16441 cagctggatt tgggatggta attttgaaaa ctttgccgat atggatatcc cagctgttat
    16501 ttctggagga gaccgtcata cagatatgac tttacgtcta aaagttgccg ggattccaga
    16561 aaataaattg acagaaacaa aagaattacc agaagtaatc gaaaaaatca aagaattgcc
    16621 aactgagcat gtctatattt tagctactta cactgctgta ttgcagcttc gtaaagaact
    16681 agctgctcaa ggatttatca aaggaggaat gaaccgtggc taattatgaa ctcaatatcg
    16741 cccaccttta tggaaatttg atgaacacat atggtgataa cggaaacctt ttgatgttga
    16801 aatatatagc agaaaaaatg ggggtttctt gccatacgga gatcgttagt atacatgaac
    16861 cttttgatgc tgataaatat gacttggttt tctttggcgg tggtcaagac tttgagcaat
    16921 taatcatttc aaaagacatc cagaaaaaga aagattcttt gatcaactat atcgaaaatg
    16981 atggcgtcat gttagccatt tgtggtggct accaattgct aggtcactac tacatgggag
    17041 ctaatggaga aaagatccaa ggaatcagtg cactggatca ctacacgtta agtcaggaaa
    17101 ataaccggtt tatcggtgat atcgttatcc ataacgaaga attcaatgaa acttattatg
    17161 gatttgaaaa ccacaatggt cggactttct taggtgaagg tgaacgacct ttggggaaag
    17221 tggtacaagg tcaaggaaat aatggtgaag accaatctga aggtgtcatt taccgaaatg
    17281 tcttcggttc ttatttccat ggacctatcc ttgcaagaaa tgagaacctg gcagctcgtt
    17341 tgatccgcct agctttagaa caacgttacg gtaaagaatt ggatctgccc tatcttgaag
    17401 gacaaacagc ctagttattc gaaaaagggg aatgtgacaa aaatttcgtc acattcccct
    17461 ttttcgtatc ctattttaag cgacttgctt ggcataacgg ttcacgtttt gcttgatatt
    17521 cccgattgtt ttcggatcat tgattcctgt gacttcttga atcaattgtt ccaagttttc
    17581 atggatcttc atttcatgca attgacggct ttgttcatct tcaggatcat agtaattaaa
    17641 aataatcccc attgttgccg tcaaatgtgg gcaagtcaag ttatgttctt gtagttctct
    17701 cactggacgt gtaaatcttt cttgataacc taacttacgt aatggtgtcc gagcgacacg
    17761 tgtgatcgca tcagagatat ttttgttttg gaagcgatga atgatttttt caatatacgc
    17821 atgatgctgc tctgcatcaa acccccattt ggcaatcagc aaacttcctg tttcctgcaa
    17881 aactgatttt aattgtgcca caactaacgc atcttgcatt gcttcatcaa tggtctggta
    17941 acctaacaag gcacctgtat aagcgacagt cgcatgacca gtattgacac taaatagttt
    18001 gcgttcgata tatggttcta gatcatctac atataaaacc ccttttaatt ggattttttt
    18061 attttttctt tggctatcgt caatgaccca ttctttgaaa ggttccactt ggacgaataa
    18121 aggatcttca tggtgttgta atgggacgat tcgatcgact gctgcatttg gaaagccgat
    18181 atatttgtca gtaaatgatg tttccggaag atatttatac acttcttttt ctagaaatgc
    18241 tgaaccgcca atcatatttt cacaggcaat gatatctaaa gatgttgtgc ttccttcctt
    18301 ttctctttct tggatacctt ttgcaatcaa ctcagcaata aaaggtaaga tattcgggcc
    18361 aatggcggta gtaactaaat ctgcttcagc gatttcttgg attacttttt ctggatttgt
    18421 ttgattgttg attcctttga cattttttac agtgattttt tcttttgatt catctgctaa
    18481 ttcgatttcg tattcttttc tttcatttaa agcatcaatg atcgtgtgat tcacatctac
    18541 gaaagtaatc tcaaatccat tagctgccaa aacctctcca ataaatcctc gaccgatatt
    18601 tcctgcacca aaatgtactg ctctcatttt tctctctcct taagcaattg ctagattttc
    18661 cgttacttct tctttgctga gcgcgtctgc tagctggaca acattgtcca tatcactgca
    18721 ataaagtgcg atttgctgta caagttgcaa atgctcatct ccgactcctg caataccgaa
    18781 caaaacagtc gcaatctttt cttcttcttc ggtaccaaaa tctacaccat ctggtacttg
    18841 aacgacacag atgccggatt ttttcacata tttttttgct tcatctgttc catgaggaat
    18901 cgcgataaaa ttccccatat aaacagataa cattttgtct ctatctacca tggcatcgat
    18961 atattcctca tcaacgcacc ctgcttctac aagcatctga ccgcaatagc ggatcgcttc
    19021 ttctttggtt ttaaaagatc gattgagctc gatcatgtcg aatggcaaac tttgcatgct
    19081 gttttcctcc cttaagctgt ctgtttcaaa tattgtgcaa tcgattcgta tttttgcgga
    19141 ttgaccatgc tgttgactga taaatgaatg gattgaggtg cagccatctg tactttttcc
    19201 gctaaacttt ctttggctac gatcagtgaa tcaggttcat cttttaaatc ttggatattg
    19261 acttttctta acgggatagt taaatggttc ttgtctaata tatctttgag cacggtcatc
    19321 cccattgttg ctgatccgga acgatcatcg tggacaagta gaatctcttt gatttgtttg
    19381 atgtttgctg aaagaaactc ttctgctggc tcattctcaa tttcagaaac tgatgttttc
    19441 tgcttgctgc ttaattcttg aatgatttca tcatatcgag gtgaattcaa aaagttttct
    19501 actgagacaa aacgcgcgct cggtgctttt tgttttgctc tttcttgtag ttcgacctga
    19561 gtaacgatca atgtattcga gtcatctgtc agttggctga ttgcttgatt ggtcaccgtt
    19621 tgaggcaagt ctgcttcctg tacttttttt ctcaagatcg aagcgcccat cgcacttgat
    19681 cccattcctg catcacaagc aaaaatgatt ttttgtacgc cagagaatgt agctccttga
    19741 ggaatacctt gggcaccttt ggaagcttgc ttagctgcct ttgtctcttg aacagatttt
    19801 tcgaagtctt catttgtatc tttcgcatcc gctttcaaaa ttactgcaga aataccaaac
    19861 gaaacagctg cggcaacaat cacgcccaaa ataacaggaa ggtaagaatt gaacggtgtc
    19921 atcgctagaa tcgcaatgat cgaccctgga gaagctggcg ctcttagtcc agcatccatc
    19981 aattggaaaa tgaaacttcc tgacattcct ccgcctataa ctgataagaa taataacggt
    20041 ttcatcataa cgtaaggaaa atagatttca tggatcccgc ctaaaaagtg aatgatcatg
    20101 gctcctggtg ctgaagattt tgctgctcct ttgccaaaca aagtaaaagc aagtaatacg
    20161 ccaagtcctg gaccaggatt tgcttcaagc aagaatagaa tagattttcc agcctctgct
    20221 acttgttcag ttcccaatgg tgtcaaaatc ccatgattga ttgcattatt caaaaataag
    20281 atttttgctg gttcaataaa aatacttgtc aatggaatca aatgggcaga taaaatgatc
    20341 tctacacctt ttgccattgc ttgagtcaat gcatcgacaa ctggaccaat cgcagaaaaa
    20401 cctaataacg ctaatccaaa accgataaga cctgctgaaa aattattgac tagcatttca
    20461 aaaccggatt tgatccgttt ttggaatagc tgatcaaatt tcttgatggc atatccacca
    20521 agaggtccca tgatcatcgc gcctagcatc attggaatat ctgttccgac aatcacaccc
    20581 atcgttgcaa ttgccccaac aactgatccg cggtcaccag caattacttt tccgccagta
    20641 taaccgatca ataaaggaat cagataagtt agcattgggc ttaccatggt agcgaaagcc
    20701 tcatttggta aataaccatc tgggataaat agagccgtta atacacccca ggcaatcagt
    20761 gctccaatat ttggcatgac catgcttgat aaagtactgc ctaatttttg taccctcgct
    20821 tttagcgaaa tgttgttttt gggaatcgtc cgttccattt tgtctctccc tctctccttt
    20881 tgatactcta attgtatccg cttactaaaa agagaaatag tcgtactttg gcacgaaact
    20941 ttgtgccaaa ggtttgagta tgtcaacaaa ataaaaagat ttcggtatca gaaaacttca
    21001 attttaaata aaactgttat tttaattttt ttataatatg caaacaataa cttttgttag
    21061 catttaattt agatgttttc cattctatta tgttattaaa ttttatattg gaggcctatc
    21121 tatgacatta ctgatcaaaa acatgaacat gaaacgtggt aaaaaacgtg tgattcgaca
    21181 attgaatctt ttagtagaat caggagaagt agctgctttg attgccccga acggatttgg
    21241 caaaacaact ttgctgaatg gcattgccca actgctgcca acaacttatg atcaattgtc
    21301 ccttcacaac atctctccta ctgcttatac agattatcgc cggtctttct tttttctaga
    21361 atcaacgaat cagttatccg actatctatc ggctgttgat tatttgactt tgatcaaata
    21421 ctattggcag agcaaagtag aatgccaaac tgttttccaa cgattgaaga tgactgagta
    21481 ccttactttg ccgattggaa aaatgtctct aggaatgaaa caacatgtct tgcttgctgc
    21541 ttacttgatc agtgattcat ctgtcatgtt attcgatgaa ccgttgaacg gattagaccc
    21601 aggaagtatt gatatcttga atagcctcat cagacaatgg cagaaagagg ggaagacgat
    21661 tcttctttct tcccacgcta tcgcgaatat ccaatcgatt tgttctaaag catgttttct
    21721 aaaaaatgga gaaatcgtca cagaatcctc aaatatggaa gagattttag ctatttatcg
    21781 taaattatat gaggtgtaac tatgtcttta cttacatttg aacgcaatct cttactaaaa
    21841 aacaaagtaa actttttatt tcctctattg ttggtcgtgc tctttgcatt cccacttttt
    21901 tttgatcata aacttgctta tacagaattc gatgaactga atcataacta tgaagagatg
    21961 caacgtttga ttgaaacgtt gaaagaagat gagaatgaga aagaatttgt ggaaagttta
    22021 gagaaaagca acaaactgat cgaagcaatc ttacatgcaa aaaacacagg gaatgtccag
    22081 caaatggtcg aagcaactta tcattatgaa aaagatatac tggatcgact gatttctggt
    22141 caaagacaag gaattccaat cattgaacaa caaaaacgtg tcgagttgct ccgttatatg
    22201 aaagaacatc agatccaaag atattctatt tttgacttgc cagcgcatct ctctttagca
    22261 aattactacg aaaatatttt ttcaggaatg atttctagtt ttttgatttt atgtataacc
    22321 gccttgtttc tttcttccat catctcttat gaaaaaagaa agcaggtaat ctctctagtc
    22381 aatctattac cggatagtat ggtaaagaaa catagcatcc gttttaccat ttattatggt
    22441 gcagctatgc taagtctcgt gatgccattt ttgatcgtct ctatactagt aataatcaaa
    22501 aatggtttgg gagacttccg ttatcctgta ggaacgatca ttggtcagga gatacgaatc
    22561 ctgccgatgt atgagtatct tttccagagc tttctgttcc tgttgttgtg ggtgctcttt
    22621 ttaagtacaa tcagttttct tctatctgct ttatttgaac attctctagt caatctttta
    22681 ggtacacttc tttgtctttt cttagcagag tatcgcttgt tttcttcgat tggatggata
    22741 gaatcaatca gtcattactt gccaacttct tatgttgatt ttcaaaatgt gatcatagga
    22801 ggagatattt tctcgccact agcttcagaa caggttactt tcatgaatgg tatcttgaca
    22861 cttggtatat ggagtatcgt tttattattc ataggtatgg gcactatcta tataaaaaag
    22921 agctattaaa acacaaaaat aataggggcg gtgacaaaaa ccttgtcacc gtcccctatt
    22981 tcaaataaac ggttttctag aataaatagt tttcaaaagc ttgcatccgc agtaataaga
    23041 acctcccaac aaaaatagct cctcttattt ttatcttggt tgaagttatt acttgatgca
    23101 aggcagcttt ttttactgtg aacattggtt atatatgtcc atgttcaaaa atcatttgga
    23161 cttcttcaat ggtttccgca tcactgagcg ccattacttg gtcgatgtct gaacagaaaa
    23221 aggctaaatc ttggagcacc gctaactgat gttctttttt taaagccact acaaataaga
    23281 gtgtcgcgat ttttggttcc tcttttgttc caaagtccac accatcaggc acttggacta
    23341 aatagattcc ttcttctact acttcttcat ctttgtcagt cccatgtggc aaggctacga
    23401 aattcccgat atagacactc gctttttctt gtctttcgat cattgattgg atatatgccg
    23461 gtttgattcc cccttgttgg gtcatccact ggccgatctc ggtcaacgct tctttccaat
    23521 tttgatagga ttgatcgagc aatatttttt ctgctggtag ctgtatcatc ttttcacctt
    23581 catttcttta tttcttcaat caggattttc gctaacaact gataaacgat ttctttattt
    23641 cctgagcgga agatctcggt gttcaagtca ttcatgatga ttgctccact gatctttcct
    23701 aaaagaatcc cgtctttttc ttggattggc gaaggcgcca gcataactaa catacgggac
    23761 aaatcgactg gctgtctatc catcccttca atggtcaacg gatgtttcaa attgaaaata
    23821 caaaagatcg gtttagaaat caatgagctg gatgtatgga ataaccccat atttgtatga
    23881 ggaataccga taggtgcttg ttcatatcgc ttaagcagcc ggcgatgaac cacctcttca
    23941 tccgttacta gtccttcagg aaaataagga aataagctgc gaatcgtttc ggaaatcgtc
    24001 tcaggattat ccagttcttg gataaaaaaa cgaccgagta attgattgat ttgatccatg
    24061 aaatgcattg tttcctcata agtatcttct atcatccact cgttattgct gttatacgta
    24121 attttttgcc tttttttctt ctcaatatgc gaaaaagctt cttttagctg cttgacttca
    24181 tcttctagca gtagcgggga gactaaaata tattttccac cgtatcctgg taaaatagat
    24241 gtagaaataa caacatcata ttcttcttgt aaatcgattt tttttaaatc agcaatccga
    24301 tagaaatgaa tttgttggat gaagggaaaa taccttttca atcgtagttc caacatgcta
    24361 gtagaagcta atccacttgg cgaaaatcct gcaatactga tattgctttc tttcggactt
    24421 cgttccaatg aattcgcaaa atgaaggacc atataagcga tctcttcttc ggataatttt
    24481 ttttcttgaa aaatccgctc tactgcttta cggattgcat tcgcaacttc ctcgtattga
    24541 accatgattt tttctaaaat cggattggtc agtgtctctt cttgtaaaac ggctcttgaa
    24601 aacacaccag ataaatgagt caacagcatt ttgtacaaag tttcatcttc ataaaaatgg
    24661 acattcgtct cttcacttac ttcttgaatc agttgtttta ctaaataagc aagctgtgta
    24721 tcgaacgtct cttcaaagaa attctgatca aatgagttcg tgaagtcatt taataatcct
    24781 gcaaaaaaga cgatctcatt tactggataa agttgttttg tctcagaagc gatccgtgaa
    24841 aacaatcgtt tagctgtctg tagtaattct ttgtttgcca gactattata tgtctcattt
    24901 gtcagtaaat gatttttcgt cacacgctcc atcgtcaatg tcagcatcaa aaccaaatgt
    24961 tcaagctttc tatcacttag attggtcttt ttttggatca attcttcccc gattactttg
    25021 cgtgcaagag atagatgttg cggatcaacg aatcgaaaaa aataattcag tgatgcatcg
    25081 aaagaaatac tatgaaaaag ttcatattca ttgatttcta attctaatac attcgctgtc
    25141 agaagccggc ggtatttttc cggtccttcg atctcgtatc cctggttccg aatgatttcc
    25201 aaaggcagtt ggcgaatact ttcttccaac tgtttgatat ctgcataaaa agtggtatta
    25261 ctgatgcgat atgattccat gaaatgagca agactcaacg gctctgtcgt cagtaacaac
    25321 tcggctaaaa tcgcgtgttg tcgtttagca gtggataagt cattactttt cccatctgta
    25381 ttttctttta attgctctaa tacttcttct gatgcctcaa tgacaaatga gcctctagct
    25441 gcttttttca aagaagcatt taccgatttc aatgagactt caagattatc taattcgcgg
    25501 taaatagtcc gtttactgac cttcaataaa ttcatcattt tttggataga tacaggtgac
    25561 gggctactga gaaattctgc taataattgt ttttctctgg gcgataaata catcttatat
    25621 ttctcccttc acgagttttt cgtattcatt cgtagctacc caatgttcaa ctagaactag
    25681 ccgagggatc ttttcttcta gctgttcagt tacagctagt tctctttgtg cgatataaat
    25741 cgtttcttct tggattttga ttttttctat tgattctttt gagataacaa actgtttttt
    25801 cgcttttttt gctaattgac ttaaaatcgt catgcccata gtttgtgagc cgcgtctgtt
    25861 gtttttatac aaaaatacaa tattagcagg cgacgttttt tgttctgtga tatctgctat
    25921 ctcttttttt tcttcccgct ttggaaattg gatcgcttct tttattcgtg acagcaagat
    25981 atctgtttcc aaaaagtgat ccagtacata gatttgactt tgaggtactt tttctcttgc
    26041 aagacccgct agttcatgtt gcacgataac cagctgattt acttggttat tcaattcata
    26101 gatagagctg tatccgacag aaagatcgat tttttctgct gctaatttct ttttcagcag
    26161 ggcggctccc atagcacttg atcccatacc tgagtcgcaa gcaaaaatga cggaatgaat
    26221 gatagatggt tccaagattt ctcccctttc tttgaccgtt ttgttctctt tttctttatt
    26281 tggtaatttc aatagtatcg ctgcacaaat gaaggtcaca aatgtactga ttgctatccc
    26341 tatacttacc ccagctagtt gtggataagg cgcattggca aaaatcacga gtagtgaccc
    26401 aggcgataca ggtgcagcta atcccgtatc taaccattga aatatcaacg tgccgcttgc
    26461 cccaccagcc attacagcta aaaatagata cggtcgcagc aagacaaatg gaaaatagat
    26521 ttcgtgaatc ccgccaatcg catggatcat caatgctcca ctcgcattta ctttttcctg
    26581 tttttctgca aaacaaagga tagaaagtaa gatccctaaa ccaggtccag gatttgcctc
    26641 catcaaaaat aaggaagagt acccgactgt ttgtgagaat tcgattccta aaggtgtcaa
    26701 tacaccatga ttgattgcat tattgaaaaa tagtactttt agaggttcta agatcagact
    26761 gacaagtggt aacagattca ttgtgactag ccaagacaca acggaggcga gcaacaagtg
    26821 aacactattt aaaactggtt caataaaaac gattccaatt atacaaataa cgctaccagt
    26881 cagtcctact agtaggttgc tgacaagcat ttcatatcca gccttgaatt tcacatggaa
    26941 tctagaaaca aattgttcat atacgatgcc ggcaagcggc ccgataatca tcgctcctac
    27001 gatttgtgga gcattggaac gagcgatcaa ccctaaagaa gcaattgcac cggcgactgc
    27061 ccctttttgt ccccctaccg atttcccgcc tgaataactg atcaagatgg gtaaaagcag
    27121 ctggatcatc caatggcata cctcttctaa tgtggtgtgc aaatcgccag acacaccttt
    27181 tgttgctaat gacaagattc cccaagcgat aaacagactt agatttggca tgatcatttc
    27241 atttaagtga tgtccagctt ttctcagttg tttcatagac acgcgactgc tgttgtttgc
    27301 tttccatttg tattccatct ttttcaccag acttctattt attgatggac ggattttatg
    27361 tagaaaaagc agcaaacgaa aaaccgtctg ctgctttcag ctatctggga actatttgcc
    27421 tggttcagac gcaatgattg ctagttcacc ttggaccgcc cactcagaaa cgccatttgg
    27481 aataatacag cttgttcctt tttctaatgg atatgttttt ccgtcaacga caaggtctcc
    27541 tgctccttcg attaccgtca tcaatgtata aggggcttgt tttttgaaag atgtgattcc
    27601 tttaatatcc cactcatata cattgaaaaa ctcagtttct aaataagtga cgatcgatga
    27661 attccctttt cggacttctt tgatttgtaa ttctggtgtt ttcgcaggga cagttgttac
    27721 atcgatcgat tgttggatat gcagctctct tgtttgtcct tgcgcatctt ttctgtcgta
    27781 gtcatacacg cggtaagtcg tgtcagaact ttgctgtgtt tctaaaatca tgattccttt
    27841 accaatagca tggatcgttc cgcttggtac ataaaagaaa tcaccttttt taactggtac
    27901 tttcttcaaa aggtcatccc agcgcccatc tttgatcatc tcagctagtt cttctcttgt
    27961 ctttgcatga tgaccataga tgatttctgc accaggttca gcatcgatga tataccagca
    28021 ttcagttttt cctaattcac cttcatgttt catcccatag gcatcatctg gatgtacttg
    28081 tacagataga tcatcttctg cgtctaagat cttgatcaat aatggaaaca ctggttctga
    28141 aggatggcca aacaattcct tgtgttctga ccatagttca tctaatcttt tccctttgaa
    28201 ttcgccattt tctactacac tgacaccatg aggatgcgca ctgatcgccc aatcttctcc
    28261 gattttatcg ttagggatat caaaaccaaa gactgaacgt aaacgacttc ctccccaaat
    28321 tttttcttga aaaactggtt ttaaaaacat aggttcctgc ataattcaaa ctcctctcat
    28381 ttttcatagc tattatagca ttatttcggt aataccgctt tctctttcaa aaagttttca
    28441 acaatttcat ctcgacatat gagatactcc gcacgatgat cgacaggatg tacgcgattt
    28501 gacaaaaata tcaacgcttc ttgtgttttt atatcaatca agaaaaacgt acctgtataa
    28561 ccagtatgaa aaagaatcgc atgaccgtct tctacatcct tttttaagtc ccatcctaaa
    28621 gaacgatagc catttttttt cggtgtttga tcttgcagta aagaatcaat cgtctcttct
    28681 tctaaaaaac gaccattacg gatttttccc ttgttcaaat acatttggac aaaagtaatg
    28741 ctgtctttca aatcaataaa gagacccgcg ttacctccat gttcagccaa aactcttgct
    28801 ttcggatcat gagtttgtcc acgcaaaacc tcacccgaag gcagttgttc tgtgggaacg
    28861 atttttttgg aaggaaaagg aagaaataag ctgttcatca ttcctaatgg ttccaatact
    28921 tgttcttgaa agatttccac tgcagtttct tggtaaattt cctctagcat caacccaaga
    28981 agaatcgtac cggcatctgt atattttact tcttttccta gttgctcacc tggttctagc
    29041 ttcaaataag cggcgatcaa ttcttcctga gatagcttat ctctgtttgg tatccatgtt
    29101 ttgatatcag aagtatgtgt cagtaaatga cgcaaagtga ttgtctgatt ttcaaattca
    29161 ggaagatatt tatgaagcgg ttgatcgagt tctatctttc catcttctaa tagacgtaaa
    29221 actaatgaag tggttgcaac gactttcgtc agtgaggctg catcaaacaa cagatcttct
    29281 gtcatcagct gcttttcagg aacgacttgg gcaaagccgg ctgtaaatgt ttctacttga
    29341 ttttctttta tgaaggcgac tgctacacct ggataaaatc cttcttccat ctttttcaaa
    29401 ataagctgtt tcgtgtgtgg atacatcttt ttctcctgct tttctatcag attcttacag
    29461 tccaagctta aagcttaatc tatgaggaat caaccttttt tacacaaaaa aactgcgcga
    29521 aaaaccgcgc agtttccccc ggtatactat tttctctttc tatctctaga ataagcgata
    29581 gtatgaccgt tcataaaatg acgagacatg cgacaatcgt taagaaagaa aaagttaagc
    29641 gtcgcatcga atgtcagttt tattcttttc ttttggctca ctgacgaacc accattcgat
    29701 tcctatagca tcatagattg tgagtactgt gtgctttttg tctaagaaga aatcaactga
    29761 tagttgctgc aagtgagcaa tcagtccttc catgctttca gtatcgatca tgaatttcag
    29821 aaaatcaagt ccaatcactt catctgtagg caaatcaatt gcgcctccgc tagcttctgt
    29881 caaaccaaca tggaaatctc cttcattcaa tacatggatg ccctcttctt cattatgtac
    29941 cttcaaacca agtacatgat taagaaattg gcgttctttt gttaagttag taacatttaa
    30001 atggattttt tcaaaataag aacctgttga tagtttactg aatttttctt cagattttct
    30061 taataatgct tcttgatcca ttgtttctgg aatttctctt tggtcgttct tattctcttc
    30121 atggtaaatt tccaattcat tctcttctgg gtctacgagc aaaatcccca tacgtccatg
    30181 atcttctaaa gcttctttga ttggataatt cattttacgg atacgacaaa ggatatctgc
    30241 catttcttct gtgctaggga taatcaatga aaaacggttc agctttttga tcacccccat
    30301 gtgatcattc gctctaggac tttcttccag cagcaacatt tcacttccag cttcttctgt
    30361 tcctaaaatg gctaattcgt tttcttctct tttcaaatca aagccaacaa cgttcttgta
    30421 aaaatcaatc attttatctc ggtctttcac ccgaatggct acacttttta aataggtggc
    30481 agcccccaat tgaaaatctt tcatatttct tgctcccctc tacgtcaagc tttattttaa
    30541 cgatggattc ttcttctttc aagcaataac tttgattttg ttaaaaaaaa tgtgagaaaa
    30601 ttcgcatttt gcttgttttt tgtaaattaa tgagtaaaaa ctcttataaa aagaggttgt
    30661 gaaaaagctg ttctgcatca agtaataaga acagccaaac aaaaatagct cctcctattt
    30721 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg
    30781 ttgtgaaaaa gtcgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat ggctttctac
    30841 atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg tttaccaaga
    30901 ggttgtgagc ctgccgttta actccgagtc acaaggagca atttcaaaaa acagcttctc
    30961 aggtttgttg actattgtga cttgtgaccg aggagttggc aggagaacac cgtttaagga
    31021 agaggttgta aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct
    31081 tctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat
    31141 tcggaaaaag ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg
    31201 tactattcta tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc
    31261 cgtctttttc catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca
    31321 caataatgtt gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc
    31381 ggttttccaa taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac
    31441 tagatgttgc ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg
    31501 tgattagctg acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat
    31561 atccttctgg caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga
    31621 tttgttcgtc tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa
    31681 acagccaagt atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat
    31741 cacgggtatc tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa
    31801 gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac
    31861 caggtttgac atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt
    31921 gaacattttc aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt
    31981 cattggacat ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg
    32041 attggatagt gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat
    32101 attgcaagaa cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc
    32161 ctagtacagc gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga
    32221 ttgccgaaat aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa
    32281 atacttcctg gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg
    32341 tttcttctac ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt
    32401 gtgatttcgg cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga
    32461 ctaacgtcaa ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg
    32521 cactagttac aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat
    32581 tgattgctcg agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac
    32641 gattcatttt ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg
    32701 tcataataaa ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg
    32761 tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt
    32821 gttgcatctg ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca
    32881 agacctttgg tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga
    32941 tgatcgcaac catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat
    33001 ttttaggctt ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc
    33061 ctgctggtcc atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag
    33121 ttgtgaatga acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc
    33181 tttgccaacc agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact
    33241 tactcgttga gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg
    33301 aagattggca tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc
    33361 tgctgttttg actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt
    33421 tcctcctcct tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt
    33481 agcttgtgcg atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata
    33541 ctgcatattg tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat
    33601 taattctcgc aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga
    33661 accagattcg atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc
    33721 agtcccccct atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa
    33781 tgcaagtttt tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt
    33841 ttttccagca aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc
    33901 caacacttca ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc
    33961 ggaaagcatc ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt
    34021 accgacttcc aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt
    34081 tgtaccgctc atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt
    34141 gattgccgta tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt
    34201 cttatcgaat gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa
    34261 acggtcagtt ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc
    34321 gatcaaaata atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt
    34381 ttgataagca agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa
    34441 aaacatttgt gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga
    34501 ggccgttcca tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc
    34561 cgtacgcaat tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat
    34621 agcagctaag atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata
    34681 atcgacatct ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata
    34741 taaatctgcc agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga
    34801 gatccgtttt acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt
    34861 cagccaagaa agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga
    34921 tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg
    34981 gatcagtaat tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc
    35041 aagcaactct tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg
    35101 actcgtatga ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg
    35161 ataaaaagaa gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc
    35221 gaatcctttg aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct
    35281 gtcacgtctc attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg
    35341 atagaaactg cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt
    35401 taagggtact ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt
    35461 aggcaaatgg aaaaatgtct gtgtcggcat atggattctc ctttctttca agatgacaca
    35521 ttgtcatatg acgctatgtc attataacac ctcctacctg ttttgcaaga catttgcata
    35581 gtttttttca ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa
    35641 ttaatgagag ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc
    35701 aatcaattag tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag
    35761 atacaagaaa taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg
    35821 ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa
    35881 gctatacagg caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg
    35941 ctgtttatat cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg
    36001 ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt
    36061 tttttagtta tttattcttc aaccttatca atatgacttg atagcctttt ctttttcgag
    36121 taaactgatt ttgttagtca tagttataac aatcatagca ataaaaggag aaggtaagca
    36181 tatggcatct gttttagtag tcaaaggtca tcccctaact gcagaagaat cgcgcacagt
    36241 taaggcattg acgtcttttt taacaagtta taaagaaaat catccagatg atgaagtgac
    36301 tgttttggaa ctttatcgcg atgatattcc ggaaatcgat gaagaattat tatcaggttg
    36361 ggaagcatta cgtgctggtg cagaattcac ttccttatca gaaagtcaac aacaaaaaat
    36421 tgctcgtttc aatgaattga ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa
    36481 cgccttatgg aatctaaata tcccaacaaa attaaaagct tggttcgata ccgtcaatgt
    36541 agctggaaaa acgttccgct atacagaaaa cgggccagaa ggattagtga caggtaaaaa
    36601 agcactgcac atccaatcta atggcggtgt atacaacgga caagactttg cctctcaata
    36661 tgtaaaaggt attttaaact ttgtaggcat cgatcaagta gatcagctat tcatcgaagg
    36721 aatcgattat gaccctgacc gtgcagatga gttgatgcaa aatgcattag ataaagctgc
    36781 tgctttaggt aaatcatttt aaaaattgag ataaatgcat acaaaagaga cagaggaaaa
    36841 aatcttcaaa gcagcatttg tgccttggat attttcccat ctgtctcttt ttgttattct
    36901 ttctgctttt tcaacggatt ttcttcataa agagaagaga ttgttgtgta ccattcaaaa
    36961 acatgagtaa tcactacttt agctgctgca taaacaggaa tccccaagat cacaccgatg
    37021 attccgaaca atttcccaga agtcagcaga actaataaaa tcgttactgg atggattgct
    37081 aactgattcc ctagtactaa tggagagatc aagcgtcctt ctattgtctg ctctactgca
    37141 aaaacgatca gtactttgat gatcatgaat ggacctgcta caatggctaa aaagatagcc
    37201 ggaatcatcg ctaaaaatga tcctaagtaa gggatcaggt ttaaaaaacc cgcaataatc
    37261 cccaacgtga cagcatactc taaaccaatg accgcaaaac caatcataaa catgattgcc
    37321 acagcaaaag ccacagttag ttgtcctctt atataagaag aaacttgact attcatttca
    37381 ctcagcacat tcaacgtcgg tttttgcatg cgtgtcggta aaaatttgac aaaataaggt
    37441 gctaattgtt taccgtcttt caatagataa aataagataa acggcatcgt cagcagagcc
    37501 actaaaatag tcgctaccgc gccgacaaaa cttccaagac tctgaactgt actcttagat
    37561 atatcttgga tcatatcgcc tgccgaattc atgaacttat cacctgctgt ttccaactgt
    37621 tcgcgaaatt ggttgaacag cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta
    37681 tctacatatt gagggaagtt actgatgaag ctcactgttt gttcctgaat cttagggatg
    37741 atcactacgc tcccccatgc gattagtgca acaaccaaaa caaacagccc aatgatactg
    37801 taaagccttg gtacttttcg cttttcaaag taatcaacta ccggattcat caagtaatac
    37861 aagattcctg ctaaaatgat aggcagacct acgattgcta aaaattgcca gattggtgca
    37921 aacaaatatg aaacttttgt aaataaaaag acgatcagca atattaacaa tacaacaagt
    37981 aaggctgtga ctacttgatt atttaaaaac cagcgccaaa accacgatac cggtctgttc
    38041 ttcttatttt ttccttccat aattcctcct gttagttata aaaaatttgg cttcccaaac
    38101 caatagcagg cagtctatgc ggatgaagat agattccatt tgcaatttgt ccttcttctg
    38161 gacaagcatc aaaaatcaat gatacgtgag ctactgtctt taaattccca ttggccatcg
    38221 gaccgacata atcgatcgta tatacctgat catcaaaagc aattgttccg cctgctttca
    38281 aagaaaaagg ttcgtctttt gatacttctt gaatcaccga atattttcgt aaggcagctg
    38341 ttgctgtttt atcgaacaaa atcagcatgg gttcttcctt gtttattgct tctgtcccaa
    38401 tttctgtaat ctttgcctga atcattgcac tctctccttt aattgctttc aaaaacagta
    38461 taacacattc tcattcttta gccgtgtgga ataattatgt gatagaataa aaacgaaaac
    38521 tatttcatta caattaggag gaatcagctc atatgatcga aaagataatt ttaggaacct
    38581 atacgcgtcg cgaaagtaaa ggaatctata cgatcgacct tgatacagaa aaagaagaat
    38641 tagccaacct ttcattagct gttgaagaaa atagccctac ctatgtagca aaaagtaaag
    38701 ctggcaatct ttacacagtt actagtgtgg atggattagg tggtgccggt gcgtacgata
    38761 aggattttca ttttttgaat gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg
    38821 ttgatgaagc acgtcaatta ctatacgggg cgaactatca taaaggtgaa gtaaatgtct
    38881 accatatatt aggagatggc gaactgaaag cagcagattc aatattccat caagaagcga
    38941 caggtcctca taaaaaccaa gatcatgcgc atgtccacta tacggatctg actcctgacc
    39001 aacgattagt cgtatgtgat cttggtacag atcgtgtgta tacttatgat gtgtccgaaa
    39061 acggcaaatt gaatcttgta actaccttca ctgcagagcc aggaacaggc cctcgccatc
    39121 ttgtctttca tccaaatgga caattcgctt atttatttgg tgaattagac agcacagtcc
    39181 gcgtgctttc ttataataaa gaaacaggtg cttttgaaga attacagaaa atatcgacct
    39241 tgccagaaga attcgacggt gaaaatggag gggcagctat ccgcatttca aatgatggca
    39301 agttccttta tgcttctaac cgaggacata actcgattgc tgtattcgca gtttttgaag
    39361 atggttccca cattcaaaac aagcaaatca tctcaacaga aggggatttt ccccgcgatt
    39421 tcgcactaga tccaagcaat aattttgtcg tgtgtgccaa tcagaacacc gataatctga
    39481 cattatatcg ccgtcatcct gaaacaggat tactggaatt actgaaaaaa gatgtctatg
    39541 taccagaatg tgtctgtgtt tattttgatc gttagtaaga aaaggagaat gaaatgaatc
    39601 agcaagacgt attagacaga ctacgggaag aactaaatat tcctttcttt gatgggaaaa
    39661 tcgaagataa agagtatagc gaagaggaat atcagaaact caaagccgat ttacagaact
    39721 actttgaaca atatgtacag aatgtagaaa actaatcagc tttccatact atctgaaaag
    39781 aacatcagga caagactttt ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc
    39841 cgagatggtt agactcgacg gaaaaggagt atttactcga ttttgagtaa gtacaaggtt
    39901 gttgttggtt ataggtatgt cattatcata aaggattttg atcactctat cataaaggta
    39961 atattacatt tatattagat aactctagtt tttattgtta tcaaagattt cttttatttt
    40021 ttcaacaaca aattagtcat tttttggatg gcagctgtca attggtctgt cgcttggtct
    40081 tgttttgctt cagatgtatt gaatagatct tcatctaccc ctgattcaag atcgatactt
    40141 tggatcattt cttcacagcc tgctacatat gaaagataag ctttttcaaa atttttatga
    40201 atgcccattg cttttgctgg tggacgcaaa gtactgactt tcataagcat tgcccgatat
    40261 ttttttgttc cttcttcaaa taacaggatg atctcttcaa gagtcgattt gtccagttca
    40321 cttgtttttc cttcatcaat tgcgtttctg atcacttcat atttcggatg cattccttct
    40381 ccgattgctt ctgtttcctt gacgatttga tcgatggact gcacataata gccaatattc
    40441 ggtaacatgt tcgttttcct actttctcca ttgttgttat tatttattct acttgttttt
    40501 atcctcaaat gacaagtact tgaaaaaatt taatggtttc tatacagctc gccattctta
    40561 ttcgtgctca aagaaaccat ctgctaaact atattgtttc cctgcgacaa tgtcatattg
    40621 gatcaattga tattcgtcgt aaatctcttg ttgcgagcga ttgaattgcg cttctttata
    40681 ccactgtccg ttttgatagt acagttctcg ttcaaacgcc ggaagttcct gttctaatga
    40741 taaaagcagc tgataaaagc catttgttgt ctgattcgtc tgttccatta gattcgctgc
    40801 aaaataaaac ggactggtga ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact
    40861 gtcgaacata agaaattgtg tttcatgcaa tgtttgtttg tcattttttg cttggatcgt
    40921 atctgaatag atccctggaa gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag
    40981 attcttcaat tcttccgtga actgcttcag tgccgtgctg ctataggaaa tatcttgcag
    41041 ataattctct aagtcaagtg tcccacttcc atctgaagtt tttgcaggat agtctagatt
    41101 atcatacttt ccatcgtagg gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg
    41161 tgttttttct tccttgagaa gtgtcattac ttctttgtaa gctgactcat cagaaatata
    41221 tggattattc tctatcgtat ccgtataggt catcgtacgc tcgctgatga actggtcaaa
    41281 tcctaatgtc tgataaacat cctctctttt atacatcgaa gtattgtatg gatggatcgc
    41341 tgtcgtctgg tatgattgtg cgtttaacgc tgaaacgata gaaggaagct gatccatttt
    41401 tggtacaagc atcgtgtaag gggtggtgag ttgcgcatta aataacgcca tagaaaaacc
    41461 agtcaatgct tcaaattcga tatttgctgt tccaccgcca tagttttgag aaagcatatt
    41521 gccactatat gtttgatcag ctacttcata ataatctgcc aatggctcac cagatacttc
    41581 tacgccgttt aatcgactag gatctgagaa actttcactc atcacgaaca cgatgttcgg
    41641 cgactcttcc tctacagttt tatttttttc atcggctagc ttctggtatt tttcagtgat
    41701 ttctttgatc ttttctttcg aatatccttc aggttcatcc attggttcta ccttcagatt
    41761 gtacaaaaac ccaccgataa aaccagtgtt atagtagttc attttctggc tgtaaggtat
    41821 ccatagtgcc gttttattat atgcttttcg caacaaatta tcgggattgt tgaaattact
    41881 gatataccct agcagtccaa ttgccacaag cagtgtcgtt aatcggatca attggaatcg
    41941 ccttcctttt ttcaggcttc gaaacatgta ccagcaaaac aatcctatca cggttcctgc
    42001 ggcagctaag atcactgtaa acagcatagt gccggtcata tctttcagca atccgatttc
    42061 agtgatcatc ttcaaatcgt ctggataaat cggttcttgc cgataactca ttttcatata
    42121 atttgcaaaa cctaatacac cgatggctac gacatacgta agggaaccta aagggacaga
    42181 tcctgctagt gcaatcagaa aaataagaat aatcagcaag acgagacagg ctaagaagaa
    42241 tttttccgta tgccaagaaa aagcaaattt caacgcaagg tctacggata gatcattttg
    42301 acaccactgc aaaaataaat tactaaaaac aacaagaaaa aagatactga tgattgctgc
    42361 tgttcctttt aaaatctttt tctgtttggt cgaaagtctg cttatatatc ttttcaaatg
    42421 cttgctcctt catttctatc aataaaagaa tgttgcttct cttattttag ttgtacgaaa
    42481 ttcgaaagta aagaacggaa agaagaatta atgaaatcca aatattctct ttctttatgg
    42541 cctttctggt ttctttaata agcggacctt tcgtactcca gaaatttttt ctagctgttg
    42601 tttcacttga ttgagctgtt caaaatcagt actgtctatg tcaatcaata tgtagacatg
    42661 ctcattctta tggttacgcg atatatctgc gatcgctagc tcgttctcat tcagcagaga
    42721 aaatatctct gctaatatat tggttttctt ttgataaaat atagtgaatc gaaacggcgc
    42781 ttggaaaaac attcgtgcgg atggataatt gaccgcttct ctaaccgtac cgaacaaaag
    42841 ataatctttt aaggcttcaa ctgccaaacg tccgctgtct gccaaagctt cctgagtcga
    42901 tccccctaga tgaggcaaca taagtatacg gtcattttct aagaactctt cttctggaaa
    42961 atcagtcaag tatttagcca gatgttgttt ttccaatgct tgcataacag catatttatc
    43021 aacaatttca cttcttccta cattgataag tacagcatct tttttcattt tttctatatt
    43081 cgcctgatcg atcaatcctt ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc
    43141 cgaggtagaa agaactgttt ctaaatctgc ttgccgaaca taatccaact gggcatcgcg
    43201 gatagaataa cccaagacat ccatccctaa gctatagcaa cttagcgcta cttttgtgcc
    43261 tatcgctcca agaccaagaa gaccgatggt tttgccttgc aattctcgtc ccacataagc
    43321 acttcttttg ttttctgcct gttctaaaat attaggacct gttagtgttt gtaccatacg
    43381 acttgcttcg attatcggac gggaagataa gagcaaacag cacaaaacaa gctcttttac
    43441 tgcattagca tttacacctg gtgtattcat gacgatcgtc ccgttttcag aagctttttc
    43501 aacgttgatc gtgttcaccc caacaccagc tcttgaaatt gctagcaaat ctggtgtaag
    43561 ccattcatct ttcactttcg tactacgaat aactattcct tcggctggca aatcatctat
    43621 tattttgaaa tattcccctt gtaacttttc tatctcttgt tctggaatag catcaatcaa
    43681 atgaacgtga tacataaaac cccctcctta ctaacaagta tctcgaaatt tctgcaatta
    43741 gacaactgtt taccatttga cttcctagaa gcaaaaagag cagttagagc agagtaggct
    43801 gagtgataaa aataaggcta tgacaagtta ctgccataag ccttattttc gagagcaaaa
    43861 tattgagggt gagatatctt gataaatggt gttccgttag ctgacattga taataagact
    43921 aaatttcaaa aaagttgaaa agcaaatttc gtcaatctct tcttatttct taaaactaag
    43981 tggctttttc acaacctctt tttatctaat aaccactgtt tccaactcaa ctaattttgc
    44041 ccacgtttca atttgatctg ttgttagatt tagcgagaca acggtatgat gaccgccacc
    44101 tgcctgaatc catgaatgga caccttcatg gaaattcggt ttagttttcc ataacacacg
    44161 tgcaacaggt aaattaggtg cttcagtcgt tggttcaaag gcttctactt cattgatcag
    44221 cagtttataa tgcgtaccga aatctgccat cgatacgact accccttctc ctgctttccc
    44281 atcaaataca agacgcgccg gatcttctct atctcccatt gataaaggag aaacaacgat
    44341 tcttggtttc gtcgcagcaa gcgtcggatc tacttccatc atatgggatt ccaagattgc
    44401 ttcttggcca ctagctagct cgtaagtata atcttccatg aatcctgtat ctttccctcg
    44461 agccatgatt ttcagcaacc gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa
    44521 accgtatcct tctgccatca aacgttgtac agctagtcca ggtagttgtt tcattccata
    44581 caggtcttca aaattagtcg taaaagcgtt atagcctcct tcttctaaga aacggcgaat
    44641 accgatttcc tgctgagctt gtacttttac atgttcctcc catgtctttt cttcataatc
    44701 accatagtcg aaggtataca attccttgta ctcttcaaat aattgatcga cttcttcgtc
    44761 tgatacgcga tcgattactt gtacaagatc tccgatacca aaataatcaa cagtccaacc
    44821 aaattgaatc tgtgcttcca ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc
    44881 accaaaacga gccactttta ttccaaaact ttcattatag gctactgcaa catccatcca
    44941 atcagcaatc tctttttgga tctcaggtcg tttccagtag ccaacgacga ttttattttg
    45001 tttatttaat cgagcattga tgaacccata ttcgcggtca ccatgagcgg attgatttaa
    45061 attcatgaaa tccatatcga tcgttttcca aggaatgctt tcgttatatt gcgtagctaa
    45121 atgaagcaat ggtttttgta gaagctttgt cccgcggatc cacatttttg ctggtgagaa
    45181 agtatgcatc caagtgatca cacctgctac ttcttctcga taattgacct ctttcatgat
    45241 ttttgtgatc gtatcagcag taactgctaa ttcttgaagg cggattggat aaggcaattg
    45301 gccattttca ttcagttcat cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc
    45361 ttcttcccca tataaatgct gtgatcctac aacaaaccaa aattcttttt ctccaatatt
    45421 caacataatt agccactcct tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt
    45481 tcgtaaataa tgtttatcta aaatccgttg aggcagctct tctgaaaatg aattgagctg
    45541 tctggtaaat aaattcatct tgcatacttc atctagtacg actgcgttca ttaccgcact
    45601 ttgcgcatct ttcccccaag taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc
    45661 taagggatca attttgcgtt ctttgaatgt ttctatgata acttttcctg tttcatattc
    45721 gtacccgctg tcgatttcct gttgtgtcaa aaatcgagca caaggtaccg atccataaaa
    45781 agtatctgca tgcgtcgtcc ccatcgcagg aacatccaat cctgcttgtg cccagatcgt
    45841 cgcccatgtc gagtgagtat ggacgattcc tccgatttga ggaaatgaac gatataaaac
    45901 agcatgagta ggcgtatccg aagagggatt gagtttacct tcaatgatat gattgtccaa
    45961 gtccacaacg accatgtcgt ctgctgtcat ctcttcataa ctgactccgc taggtttgat
    46021 aacgaaatat cgtgtttcag gatcaaaagc gctcacattt ccccatgtat atttgacgag
    46081 tccgtgttta ggcaagtcaa gatttgcttg gtatacttct tctttcagtt gttccagcat
    46141 tttctatcac tcctttttct tgcaattgat gaatcgctgt tttttcaatg tcgatcccct
    46201 cacggtaacg tctcataaat atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg
    46261 tgattccttg gtcttgtcca aacacgtctt ctgccaagaa gttttctaaa gacttcccat
    46321 gtcgatccat catataggct gctaataacg cgattcccca cgctccacct tctccggctg
    46381 tgtccatcac tgtaactggt gcctccatag cagatgctag aatccgctgt ccaacttctg
    46441 gtgttttaaa aatcccccca tgccccacta aacgatcaat ttggacattc tcagatttca
    46501 atatatccat tccgattctc attgcaccaa aagcactggc taaatggatc cgcatgaaat
    46561 tcgctaaatc aaaacggcta cctggtgtac gtacaaataa tggccttcct tcatttactc
    46621 ctgtaatgtt ttcaccggaa aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc
    46681 cttctagcgc tttgttgaat aataccgtga ataatttatc tctgcttatc tccataccca
    46741 aagaattggt aaattcttca aaaatactga cccaagcatt gatatccgaa gagcagttat
    46801 tcgtatgaac catgcctact agttctcctg aaggtgtagt gaccatgtcg atttccggat
    46861 ggacattttc tagttctttt tctaatacga tcatagcgaa ggcggaagta ccagccgaaa
    46921 tgttccctgt ccctttggct acactgttcg tagcgaccat accggttcct gcatcccctt
    46981 ctggcggaca aaccgggatt cctggtctca aatttcccgt agggtcgatc agataagccc
    47041 ctgattcgct taacttgcca gcttcttctc ctgctactag aactttaggc aaaatgtgat
    47101 ccaagtcaat cgctacgtgc gctttttcga ttaactgacc aaaaatattt ttcatttctt
    47161 gatggtaggt tttcgttgaa ggatcgatag gaaacatgcc tgatgcatct cctacgccta
    47221 gtactttctc accagtaagc tgccaatgga cataacctgc aagcgttgtc aaaaaattaa
    47281 tttctttcac atgttcttct ttattcaaaa tagattgata caaatgagcg atactccatc
    47341 tctgcggaat attgtattga aatttttccg ttaatattcg ttctgcttct tcagtgattg
    47401 tatttcgcca agtacgaaat ggtactagta actgttcctt gctatcaaat gctagataac
    47461 cgtgcatcat tgcactgaac ccaatcgacc cgattgtcgt aagattctcg ccatagattt
    47521 cgaatacttc tgctgccatt tttcggtaac tgacgcgcag tcctttccaa atatcttcta
    47581 atgaataggt ccagatacca ttttctagtt tgttttccca ctcataacta ccagcggcaa
    47641 taggagtaaa agatgaatca atcaatacag ctttgatcct agttgatcca aactcaatcc
    47701 ctaaagatgt acgaccatct atgacatcta acatactttg cgcatttact tccttcataa
    47761 tgatttaact ccctccttct cgtcaaacct gatcaaaccg tctttttagt agcgcttaca
    47821 atgattattg tatacttttg tacgtacatg tcaagcagtt atttacaaga atacaataaa
    47881 aaacacttgt tttgtacgta cataagttat aaaatgaaat tgcttacaaa atctgatcaa
    47941 ccgccttctc gaaattctag aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct
    48001 catcgtttat ttattttttt ggcgcatttg gcggcattct atttggatat gatattggtg
    48061 ttatgaccgg cgccttacct tttttacagc atgattgggg tttggcaggt aaagcttctt
    48121 tgataggatg gatcacgtct tcggtcatgt taggtgcgat ccttggcggc tctttatctg
    48181 gtcttctttc tgataagctt ggacgacgaa aaatgattct tctttctgca cttattttta
    48241 tggctggttc tgtcctttct gcatctgccc ctcacaatgg ttcttatttt ctgattgcag
    48301 caaggattct tctaggttta gctgttggcg ctgcatctgc cctagttcct gcttatatgt
    48361 ctgaaatggc tcctgctaga ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg
    48421 cttctggcat gctcctttcc tatgttgcag attatttgct aaaagggttg cctgaaacga
    48481 tggcttggcg cgtgatgtta gggctcgctg ctgtccccgc gctgatttta tttttcggtg
    48541 ttttagctct accagagtca ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa
    48601 aaagagtatt gaactatatt cggacaccaa aagaagctga acaggaattc gaacaaatcc
    48661 agctgaatgt caaacaagaa aagacaaccg gaacttcttg gcatactttg tttttagaaa
    48721 aatatcgttc cttagtcatt gccggtattg gtgtagcagt attccagcaa tttcaaggag
    48781 ctaatgctat tttttactat attccgctga tcgtagaaaa agcgactggg catgcagcga
    48841 gcgatgcatt gatgtggccg atcatccaag gaatcatttt agttgctggt tctttgctct
    48901 ttttggttat tgcagacaag ttcaatcggc gaactttatt gaaaataggc ggtagcgtga
    48961 tgggactttc tttcatccta ccagctgttt taggaactgt actggatgct catacaaatt
    49021 cgttgttgat tctcttgttt ttatgtattt atgtcgcatt ttattcttgc acatgggctc
    49081 cactgacatg ggtaatcgta ggcgaaatat tcccactagc tgtccgcggg cgagcatctg
    49141 gcctagcctc ttcttttaat tggattggtt ctttcctagt tggcttgctt tttcctgtga
    49201 tgactgcttc tatgtctcaa gaaatcgttt tcggcatttt tgggatcatc tgctttttag
    49261 gggttttatt tattcaagag atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg
    49321 aacaaagtgc atctaaaaaa acttatccta aacgtatttc tatccatcat tcttaaagag
    49381 gttgtgacag aagtggtcag cttcaagaaa taagaaggaa ccgcttagtt tccagtcaca
    49441 aggaacaatt tcaaaaaaca gcttctcatg tttgatgaat attgtgactt gtgaccagtt
    49501 ggtaggagaa caccgtttag tggagaggtg gtgaaagaaa cgttcagcct tgagcgatga
    49561 caaaagctct cacaaacatt gcgacgagta accgtaggag caataagagg cggtaaaaac
    49621 ccgtctctta tttttcgttt tctttcactg tttcccgttc aactagtaca ggttcataga
    49681 gaatatttgg ctgtgcctgt cctttttcaa tcgttgtgac gatccaccgt gcggcatctt
    49741 gtcccaactt ttcttttgga tgcgtcaatg ttgtcaattt tttattccct aaatggctta
    49801 aagaagaatc gtctgttcct acgatcgata ggtcatctgg aatatgatag ccattttctg
    49861 tcaacttatc tagcattaaa ctagctattt gatcgttata gcaaatgatg ccatctaaag
    49921 cttgttcttc tttcaaccgt tgtactgttt tttcagcgat tgtttctcgt gtttccgtgg
    49981 tgaaagtaat gatatcttcc gtcaaaaaac gcacattata ctgttcacac gcgctgataa
    50041 aacctttcat cctgtatttt ccttgaagat cgtcgatttt tgtgatgaac aacagacgct
    50101 gatgtccttt ttttatcaaa tattctgctg ccataaaacc gactttcaca tcatctaaac
    50161 aaatataaga ggcatttaac tcttcataag cagcattgat catcaccatc ggaatgcctt
    50221 gttctctcaa agcaacataa agtgctaagt ttgggttata ctgattactt ttcgtaggct
    50281 caacgatcaa accatctacc ccataagcaa tcattttctt caaacactct ttctccaatt
    50341 cgtgatcgtt attcgtactg gctaaaagca atgaataccc agattctcgt aattcatttt
    50401 cgattcctcg gataatcgac ggaaaaatat aatcagagag atatgtggtg atgacaccga
    50461 tcgtttttgt tctgttcaat ggtgtttttt ctttcttata gctgtcatct acatatgtgc
    50521 ccgacccttt ttctttacgc agatagccct catttaccaa cacagcgatt gcttgccgaa
    50581 tcgtatgtct gctgacgtca tatatttctt gcagcttgaa ttctgaggga atggttgtat
    50641 ttacttcgta ttcctcacga aggatctttt gttttatctc ttctgcaata tcttgatatt
    50701 ttgccatttg ccttgcccct ttttatttgt acgtacatat atcataacga gtctgcaagg
    50761 tttattcaag caaaaatcaa ggtttcttgt gctcataagg catacggaac aggttcacca
    50821 aattctggaa atccttgctc attccacgta aagcattgcg cgttcgcgtg ccgattagga
    50881 ttgtctaacg gatccccttt ttggttcttc tctggtcgag catgatagat caaaatatca
    50941 aagcacccgt cttctgaaac cgtaaacgag ttgtgaccag gaccaaattg cttatttttt
    51001 tcagaagaaa cgaatactgg ctccgataat ttatgccatg attcttttaa taaaagattc
    51061 ttgtgctcat cagcccacag caggcccatg gcatagtttt catctgttgc actaccagaa
    51121 tacgtaatga atattttccc attgcgagta aggactgctg gaccttcatt tactgaaaaa
    51181 ccccgttttt cccattcata ctcaggtata gaaaggagga tctgcttccc ctttaacgtc
    51241 caaggattat ccatttcgga tagataaaga ttagaattcc cagtaatttc aggatcttgt
    51301 tgggcccaca cataatatag ctttccttga gaagagaaag ttgttccgtc taaactgaaa
    51361 gtttcccttt tcgtttttat ttgtcccttt tctatccaat ctgtattcat tggatctata
    51421 tgctgatttt caagtacgaa catgcggtga tgatgagatt catcacgtat agttttatga
    51481 ttgcttgcag caaagtaaat ataccatttt ccattgatat aatggacttc aggtgcccag
    51541 atcaattcac tcatcggtcc ttcttcatga gcctgccata caatcaggct tgcagcatgt
    51601 tctaattcat tcagtgactt tgcccttctt aattcaatca cttggtagcc tggaaccgat
    51661 cctgtaaaat aataataacc atctgtatgt ttatataccc aaggatctgc ccgttgccga
    51721 acgataggat tattataaag tgtcttcatc ataacaaatt tcctcacatt ttctttattc
    51781 taattacatt ccaagagtat ttttctatcc ctacatttaa ttcgtcatgt ttttctattg
    51841 aaaaatagtt tatttcaggt tttatttttt ctggttcttc acgtgtattt ttgtccaaca
    51901 gatcgctact ggttaatgtg tagtgctctc cttgatctag caaattgaaa tcatgttcaa
    51961 tcgttatctt ttgttgttca tttgtgcgat tgatcaagaa aatagtcagc tgattgccgt
    52021 tatctacgat cacactgtcc acataaggaa cattttttat cttttcacaa tcataaacag
    52081 ggcaaatgat ttgttgtttc aatacttgac ctttagcata tttcgataag tgttgcatca
    52141 cataataagt tggattcctc catgcttgtc ctttagcatc tgtcagtatt aaaggtattg
    52201 tgtttactaa caatgattga caactaatct ttacgcgatc tgcgtgtcgt aaaatagcca
    52261 gccctaatga accaaataat aatgtatctt ccatagtaaa gtaacaccaa tcgatgggac
    52321 taccgacttc aaaatcccta tgtctttttt ccggttgggc atgtacattc cattcatcaa
    52381 acgctagata cattgttttc tcacttctca aatatccttt tacataatca caagtagcaa
    52441 cgatttcttc aatttgacga tcgaatcgat aagcttttgc aaggtatgtc gctgtatcat
    52501 cacgctcatt tttaggaggt ttagtcaagt cttcttcatc atacttatca atatagttat
    52561 gcaaagctaa ataatctaca ttctcataag cttcttctaa tgcgatacga tcccattcag
    52621 ggtacgatgg taaacgtggc gtagagcttc caactaagat tgtttcaatt gaatcatcca
    52681 ctaacttcat gacttttgag gcttcatttg ctaacctacc atattcataa gcactcttag
    52741 cccccacttg ccatggaccg tctagctcat tccctaaaca ccataattta acgccaaaag
    52801 gctttttttg cccatttttt attcttaaat cactccagaa ggttcctttt ggaaaattac
    52861 aatattctac taaatttttt gcttcatcaa ttccttttgt tcctaaattc accgtgaata
    52921 tcggtttcgc ttgtacatga ctgatccatt taaaaaattc gtggagtcca aaagaatttg
    52981 gttctatacc tcgccatgct aaatcaattt tttttggtcg ttgttctttc ctacctacag
    53041 tatccaccca atcataacca gatgtaaagt ttcctccagg atagcgaatg acacctaatt
    53101 ctaattcctt tacataagag aggacatctt gtctaaaccc gtcaccatca gctgtttcat
    53161 ggcccggttc ataaatccct gtatagacga cacttcccat atgctcaaca aatgagccaa
    53221 acagccgaga actgatctct cctaccgtaa aatcctctct gatttttact ttcacttctt
    53281 cgtccaacag ctttccctac ctttcatttc ctatagtcaa gataatagat acaagtttcc
    53341 atacgccacc agcaaagata aaaataaaag gagggaaaag aaatatgagc agtacaaatc
    53401 ctaataacaa tacgcaacaa agaatatttc tcaaaaaatc aaaaaatact ttccatgcac
    53461 tttgttcaaa aatttgtaaa aatgtgatat ctttgttttc agaaatcaca tatatccagg
    53521 ataaaagaaa catggaataa ataagtaaaa ctacatatcc actaattgaa agtacgattg
    53581 tctgttcttc taataattga ttaacaagaa aataaataat gatcgttaac agactaagga
    53641 acaagttctt accaaaatgt tgctttattc cttgaaagaa aaaataaatg atatttcctg
    53701 atccctcacc tttccattgc gccattacat aaaaaacagc acatgtagca ggcacgatag
    53761 taataacagg caaacacacg attacccaga gaatacctaa taagatgtaa tcagaaacca
    53821 caatcaactg tccataaagt ttagtctcaa ctattttcat cttcctcact ctttcattcc
    53881 agacatagct gttccttgaa caaaatattt ctgtgcaaaa atataaacca ctagtaatgg
    53941 cagcattgaa ataaccgttc cagccattac aggtccataa aaagaaatgt tctgccctgt
    54001 aaataaagct aaccctgctg gtaaagttct catttttgaa ctacttgtca atatcaatgg
    54061 ataaagcaag tcattccaac tattcatcag cgtgaaaatc cctaatgata aaagagtagg
    54121 tttgctcaat ggcaacatga ttttcaaaaa aatattaaac tcacttaatc catcaatgcg
    54181 tgctgcctct tccaaagaat ctggtaaagt aataaaaaag gaacgcatca tgaagatacc
    54241 aaatgcagtt gtcatacgag gaatgatcaa acctgcataa gtatcaagca tgccaagcaa
    54301 attgacttca ataaatagag gaatcatgaa gacttggaat gggatcatca ttgtcagcaa
    54361 aacgaaatag aacaaaactg attttccttt gaaacgcata cgagcaaatg cataccccgc
    54421 aagagaatca aagaatacag aagtgatcac tgtccctcca gcaaaaatca ctgtattttt
    54481 gatgtaatct aataaaggga tcgttgtcca aaccttaata tagttatcta gggtatagct
    54541 atctgcaaat attctaggcg gaaatgagat aatgtctttt tcgtgtttaa aagaagaaat
    54601 aatcagccac aaaaaaggaa acgcaataac caacaatata actagcatac taatcgttcg
    54661 aatgattttt ttaccactaa ctgcttttcg actattcaca gtaactgtaa tcgagctttc
    54721 tggtgccata caggtttccc tcctatttca tttgtttttc tttccgtgac atgtacacat
    54781 tagtggagat agctatagtt gcaacaatga taaacaaaac cgaagacagt gctgatgcat
    54841 agcccaattc ataaggtgct gtaaatcctc gactgtaaat atattgaaca gctgtttctg
    54901 tcttaaattg tgggccacca gcggttgcaa catatacttg gtcaaacact tgcatcgaac
    54961 caatcagatt cgttaaaata caaaaaccta aggagggaac gatctgaggt agcgtaatgt
    55021 taaaaaactg ctgtcgattt gttgcaccat ccatttgagc agattcatac aaagagggag
    55081 agattccctg aatagcagtc aagagaatcg tcatagtaac accaaaattc ttccaaacag
    55141 tcatgatggc taccgtcggc atagcaaggg ttggatcgcg aaaaaattga gggatcggca
    55201 agccgacttg tgaaaacaag taaggaatcg cccctacatt cggatcaagc aacatagaaa
    55261 aaataatcgc aattgacgtc aatgaacaaa taactggaat gtaaaatgtc gaacgacaaa
    55321 aacgattgaa tctggtattt ttagataatg ctactgctgc taataaacca acgataattt
    55381 gtgttggcgt ttcgagtacg gtgaaatata aagaatgaat caacgaatta actgctcgtt
    55441 catcactaaa tatacgtgtg aaattttcaa aacttacaaa atttcttgat gtaaagaaaa
    55501 tattcaaatc tgtaaaacta atagcaaatg ttcctaaaag cggtatgaca gtaaataata
    55561 ataaaactaa aaaagatgga gttaaaaaca aatacgcagc acgatcagga cgattccaat
    55621 atttggtgaa tccgttcata tagtccacct catctttcat tttgataaaa tatacaatct
    55681 tggtttaaac gaatctgaat aagatatccg aacaatcgct gtgatttttg caataacacc
    55741 tatctgcaaa tactaggtcg atcattcaga tatctgtatc ttgccaacag tctatcctgt
    55801 ataaagttct gtactatttt tgcagtaatt gattgatggc atcatctgca tcgttcatga
    55861 gtgtctcagg atcttctcct gctaataatt tttcaatcag tggattgatg atatcgttat
    55921 tgatcgcaga gatttgggta aaacctggta aatatggctc tgcatagtcc atttgtttcg
    55981 ataattcaga tacaatagga tcatttttta cttcttcatc ttttgctact gaatccaagt
    56041 aggcaggaaa accattttcg atactccatt ttttaccaat ttcagttgta ttccaatatt
    56101 tcaaaaactc atagacagct tcttttttat tattatctgt acttgccgga atggcatatc
    56161 ctacgccatc taaaatagct gtttttttgt ctgaatcact cccttgtggt aaggtagtca
    56221 cattataatc aatctcgttt gctttcaatc cgttatttaa ccatggccca ttgatttcaa
    56281 tggctagttg accagcattc attaaattgt ccatttcggc gccagaaatc gattctggac
    56341 cagtcttgtc tacatggatc attttttgga tcgcttttaa cgttttaagg ttctcaggag
    56401 acgcaaattc agacttagta taatcatcgt tcactaattt accgccattt gctaacatcc
    56461 agttatataa cggtgcagtt ccatccttat tgaagacaaa acctgataca tttttactac
    56521 tatcagttaa ttttggtgcc atttctgtca attcttccca agtagtaggt ggttgttcag
    56581 tatctaatcc agcttgttta aacaaatcat tattccaata catatacatg ccttgtacct
    56641 gcatggggac aaaatactgt ttaccttcta cttcacctaa ttcaatagcc gtttctttaa
    56701 aatcagattt atctactcca tcatattccc agaaatcgtc taatggttgt accgctccgt
    56761 tttctacata ctgtgcaaaa tctggataat ttaatgcaat aaagtcaggt gcttttttag
    56821 aagaaatcgc tgtaggaagt ttttcattca aattggccca agtcatgata tccatttcaa
    56881 ttttgatgtt tttatcattt gttttattaa attcattaac gatttcttgc aatatttcac
    56941 catcagatgc agtaaaccca ttccaaaaag tcaaagtcac ttctttatca gaatcagatt
    57001 ctgctgaact tccgccaccg caagctccta aaatacctgt tgcagctacc aacaaagagc
    57061 caattaacca ttttttacct ttcatttttt gttcctcctt catctgataa acgacatctg
    57121 accacattcc aagatagcgg tttcaataaa caattgattt tcccatcttc agcttttaca
    57181 tttgttgaat gacttggttt tacttgttca cttttaggaa gattagttgc ttttgtatca
    57241 aatccactca tttcagaaaa atcaatcact ccctctaatg ataattcaga caagtgaaca
    57301 gttagattca tttcttcttc tccacggtta actgcaaata tgacaagttc tttgtcattt
    57361 attacggcta tactttctaa atacggcact tcttcaaaat cttccgtcgc atagtttgct
    57421 gaacgaattt gtgggcttaa cacctctcct tgtccataca cagcagcttg cataaatgga
    57481 taatagatcg tctgtctcca agcttctccg tttgtttctg tcctgattgg tgcaataaca
    57541 tttaccaatt gagcaagaca agcaatcttt actctatctg catgtttcaa taaagtgatt
    57601 aatagacagc ctaatagtaa tgcatcttca aaattatagt tatcttctag taaaggagga
    57661 gcaatctgcc aaggcgctgc ttgctcatct tgttccgctg aatgatacca aatattccat
    57721 tcatcaaagg acaggttgat ctgcttctta ctatgtttct tcgccttgac tgtatcgcac
    57781 atcgctgtca cttcagaaat gaaccgatcc atgtctaagg aacgtgcaag ataatgagaa
    57841 agatcatttt tttggttgcc ataatattga tgcagagaca aataatctat ttgatcgtac
    57901 gcttgatcaa gaactgttaa ctcccaatca ccaaatgtag gcatttgccg attcgaactt
    57961 ccgcataaaa ccacctcaat tgaatcatct acgagtttca tcgccttagc cgtttcctct
    58021 gctaaccgtc catattcttc cgctgtttta tgtccaatct gccaaggacc gtccatttca
    58081 ttccctaaac accatgtctt aattccaaaa gggctctctt gaccattcga acgccttaaa
    58141 tcactccaat aagttccttt aggaaaatta caatattcga ctaaattacg agctgcatct
    58201 actccacgag tacccagatt gacagccata ttgatttcgg cccccacatg ctgacaccag
    58261 ttataaaatt catgaatccc aacttcattc gtttcaattg tccgccaagc taaatccaac
    58321 cttctaggtc tatttgctat gggcccaata ctgtcttccc aacgaaatcc tgacacaaaa
    58381 tttccaccag gatatctaat catgggaatg ttcaattctt ttaccaagtg aatgacgtct
    58441 tggcgaaatc cacttttatt agctgatgga tgatctggct gataaatccc ttcatatact
    58501 gctcgtccca tatgttcaat aaatgaactg tacaaccgcg gatcaacttt gcctacacgc
    58561 ttgacactac taatcaacat tgtggcttcc ataactgacc tcctttaatt tatacatgaa
    58621 taatacacct cgttgaaagc gttgtcaata atataaatgt taaacttact tattttttat
    58681 cgattttaat taaccaaaat attaataatg attaaaacaa ataatttaat ataactttta
    58741 ttaacaaata tgttaatata taagaaaaac cttttaagag gtgatatata tgcaactaca
    58801 aataaacgaa gaatccttac cggtatatga agcattagct agtaaaacaa ggatcaagat
    58861 catccagcta ctttccaaaa aaaagatgaa tgtcaaagat ttggcaaaag aacttggagt
    58921 gagcagtgct atcacgacga tgcacgtgaa aaagctagag gaagcaaaca tcatcaaaac
    58981 agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc gataaaatcg atatcagttt
    59041 tccagaaaaa atattcaatg cttttgatac gaaagaaaca tcgataccta ttggtcacta
    59101 tacaaattat gcaattgaac caacatgcgg tttagctacg atccatgatt ttatcgggaa
    59161 agtggatgaa ccacgctatt ttatggatcc tcgaaggatg gatgcccgca ttctctggtt
    59221 taccagcgga tttgtcgagt atcaggcacc taatttttta aatacagagg atacattgga
    59281 gatgttggaa gtttctgtgg aaatttcttc tgaatttcct ttttcaaatg acaattggcc
    59341 ttcagatatc actttcagcc taaatggtgt ggagctagga acatggacga gccctggaga
    59401 ttttgctgat atccgcggta aatatacgcc tgattggtat ccagataatc ttaatcaata
    59461 cggcttgctg aagacgatcc gaattaccaa acacttgacg aatatgaatg gagaaccctt
    59521 gtcaaacatc acgatcaacg acatccctaa agagcaagac acttggcatt tgcgaatcga
    59581 agtgaaagac gatgccaaac atgtgggggg ctgtacttta tttggtaaag gattcggtaa
    59641 ttacgatcag gatatcaaat taaaagttta ctacagctga tatttttctt ttctatttta
    59701 taaaacaaaa acagccaatc cctatgaagg gacggctgtt tttgctttat tgttatttcc
    59761 agaaatcatc caaaacggaa attggcaaat gtcgtttatg ttgtgtcttg ttccaccatg
    59821 cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc ttctagataa tcatcaattt
    59881 cattatatgt gacacctagt gcgacttcat ctgcaatcat tggtttccca tcttcaagat
    59941 ctgcagtcgg aatttttgtg tataattttt ccggtgcatt caatgcttga agcaattgtt
    60001 tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc ggctccacca tcaccaaatt
    60061 tcgtgaagaa tccagtaatg ttttctgcag catgatctgt ccctaatacg gctcctgctc
    60121 tttctccagc tacggcatat tgcgtaatca tacgttgacg tgctttgata tttcctttgt
    60181 taaaatcgct gattcccaca cctgcttcgc ttaatacttg tacttgtgca tcaacagctg
    60241 gtttgatatt tactcgcaga ctaacatcgg gctggataaa gtcaagagca gcttttgcat
    60301 cttcttcatc tgcttgttca ccataaggaa gacgaacagc gataaattga tagttttcat
    60361 cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc caaacggcct gctaaagtag
    60421 aatcttggcc gccgctgata cctagtacaa aagttttaag aaacggatat tttttcaaat
    60481 atgctttcat aaagtcaata cttttacgta tttcttcttt tggatcaatt gttggtttga
    60541 cacctaattc attgataatt tctgtctgca ggctcattta ttctcctcct atgcttctgt
    60601 atgaagttct gctacgcttt ttttcattcg ttccatgata gccattttat ggttccagca
    60661 ttcagtagac agatcgactg ggtatttttg cggattcaag tctcgtttat actcttccca
    60721 tagtgaatct agattttctt ttgtatattc tttgatttcg tttagtgcag gtaattcgta
    60781 aactcttttt ccttcgataa agatatcttg taaaactggg cgagctgtaa agtctcgaac
    60841 agttttattg ataaacgtat gcactggatg gaacatgaag atagcttctt cttctcgagg
    60901 gtcttcatcc cataatgtca cgtagtctcc ttcagatttc ccatcaaaat ttcgagtgat
    60961 gcgccacact tgttttttcc ctggagtagt tactttttct gcattgcttg ataacttgat
    61021 cgtatccatc atttttcctt catcatcttc gatcgatact agcttgaata ctgcaccaag
    61081 ggctggctga tcataggctg tgatcaattt tgtcccaaca ccccatacat caattttagc
    61141 tttttgcatc tttaaattca aaatcgttgc ttcatccaag tcattcgaag catagatttt
    61201 tgcttccgta aatccggctg catctaattg ttcccgaaca cgcttagata tataggccat
    61261 atctccactg tcgatgcgta cgccttggaa gttgattttg tcacctaatt ctttcgccac
    61321 gcgaatcgca cttggcacac ctgatttcaa cgtatcataa gtgtcgacta agaacacgca
    61381 gtctttatgg gttttagcgt aggccataaa tgcttcataa tcatttccat aagattggac
    61441 taacgaatga gcatgggtcc cgctagctgg tatcccaaat atcttcccag cacgtacatt
    61501 gcttgttgca tctgctccgc cgatatatgc cgctctagtt ccccagactg ctgcatccag
    61561 ttcttgtgcc cgtctagttc caaattccaa tagtggatct tctccgatga cagatttgat
    61621 acgagctgct tttgtcgcga tcaacgtttg gaaattgacc atattcaaca gtgctgtttc
    61681 tactaattgg cagtgcgcta aaggcccttc cacttggatt aatggttcat ttgcaaaaac
    61741 tagttctcct tcacgagcag aacgaactgt tgctttaaat tcaaaatttt gtaaatactc
    61801 caaaaaacct tcaggataga cttctaatcc acgcaagtaa gcaatatcgg aatcattgaa
    61861 cgtcagattc tctaaataat tgactaaccg ttccaatcct gcaaaaaccg cgtatccgtg
    61921 gttaaaaggc atttctcgaa agtaacattc aaaaacagca tgtaaatcag ctcgacctaa
    61981 ttcccagtat gtctgcatca tattgatttg atatagatcc gtatgcagcg tcaaactgtc
    62041 gtctgggtaa gtattattca tcttgtatta ctcctcttgg caagttttca tttcctaccc
    62101 attataacaa aaaaaattaa taggaaaaga aatcttccat tttttctgaa aaagcacatg
    62161 ttttacggat atttcttgct aaaaatgaac tccatctgct aaaactatcc tttcttattg
    62221 gtatttaacc taaacgagaa agaatactta ttttattcaa tgtatccgag acacgaagaa
    62281 attagcgttt tctatttttc ttacttgata aaataagtag attttcctac tacaatcgta
    62341 gccgttaaga taccgatgat tgaacaaata taaccaaatg tctctacaac ataatcaatg
    62401 gcaaaagttt ttgaagcaat aatacgtctt tccttttttg cttcttatcc ccaaagaaac
    62461 ggatataact tattatgtat tgaatttttt tggaaaggcg aattatgaca agatatacct
    62521 tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt aaaaagatac aaagtaagaa
    62581 agcagctgtc aatagaatgg aaacaaaaat ggcgagacat caatcagtct cgccttgttt
    62641 tttattgtat acattagata tggagcaggt taccataaag atgattatta gtcgttatta
    62701 aatagaatcc ttcgtatttt tttgttctat cactacaata tctttatttg gacgtttgaa
    62761 ttgtattccg gcacgttcta gctcctgttt cactcctaca tggactttga catcttctcg
    62821 tgtcattttt aaaacgtgat gaatcgcata gtcgatttct tgccttgtga tttttttctt
    62881 cgtattgatc atcacgactt gctttttatc atatgtgtct accaatagca gttgtgttcg
    62941 atctaacgaa aataatttgt aataattaat agccgtattc ccaaaaaaga attccactac
    63001 tttaggataa aatgtcttaa tatttaaatt actattcaca ggtgtttcga ttagcttcat
    63061 ctcattcctc ttttctatat agtcgattta gatccaatct taagttatgt taccatcttt
    63121 cctagcatta aggaatcggt tacaattgaa cctttaaaga caaaaggatt taccttttat
    63181 cacttaatag tataacataa tttcgtgaga aaaaaataca attttttctt gaccatctcg
    63241 ttacgtaaag gattatgctt acatttgtca ggaggaaaaa taatggagta cacaataaag
    63301 aaaatgtcag agatatcagg agtcagtccg agaaccttgc gcttttatga tgagattgga
    63361 ctgctaaaac cagctaggat caattcttca ggttatcgga tttatggcaa aaaagaagtc
    63421 gatcgtctgc aacacatctt attttatcga acaatggcat tcaagttgga tgatatccaa
    63481 gaggtcttag acaatccatc ttttgaccac cagaaagctt taatcaagca tcagcaaatg
    63541 ttgctggaaa aacgggcaca aatcgatacc ctcttgacaa ctgtccaaca gacacttgat
    63601 atgtacgaag gaggaagaaa aatgagtgac aaagaaaaat ttgaagggtt taaacaacaa
    63661 aagttgaaag aaaatgaaga atcctatgga caagagattc gacaaaaata cggaaaaaaa
    63721 aaggttacag catccaatga aaaatggatg aatatggata aagaaacgta tgaagaaatg
    63781 caaactgttg aaaagcagat gttatttgat ctcactcttt acttaaaaga accagaagat
    63841 gaaagcttag cagaccgtat cttccaagct cataagaagt ggttctctta cagctggcct
    63901 gaatatcagc caaaagcgca taaaggacta ggaatgatgt acgaagccga tgaacggttc
    63961 actgcatatt atgacgagcg aagtggtcat ggtgcagcaa aagctttaaa tgaaattatc
    64021 caaaaaaagg cgtaatcttt tacatgatcc aacaaaaaag tacacgtaaa atgacctaat
    64081 aacaaaacag cagacaccca agttagattt ttatgctaac ttttggtgtc tgctccattc
    64141 tttcacaagt tttttcatcc tactttaaat atattcatcg ttgaataaac gtataaggta
    64201 ttttcacttt atcctttttt tctgtaagaa acaattgaaa tgccatttct ccgcaagctt
    64261 ccagatggtg gtcgatggtt gggaaaccaa gaagctcgct gatcagcaaa ttatcgcgtc
    64321 caatcaccac tggtgtctta tcttgcagat agctttgcaa gatgcctgct gcaaccatat
    64381 ccgaatttgt aaagacacca tcaactccaa gctctttaaa aaagaagcct gcttgttttc
    64441 ctttctctgc atctatacaa tcccaaaaaa caagtgattc atcaaaagat ggaaaaagtt
    64501 gatgacatag ctgcaaagtg atttttgagt tataacttag cctgccactt cgccccaacg
    64561 tgatgcccaa ttgctttata ccttgctgct tcaaataagt aagaccttct gtaattgatt
    64621 gttctcgatc aataaagaca cagcttgcct gttttccctt gatttcttca caaaaaacta
    64681 gaggtccata tttttgatat gccaataaat catctagtgt attggcacga gacgtaatga
    64741 tcaatccatc gaaagcttta gctgcaaatt cttcaagata ccttcgctct ttttctttat
    64801 gatagcccgt cggtaaaagt gtcactttgt aatcataatc aaacgctgct tcaatgatcc
    64861 cactgatcaa ctgatcaaaa tacggatgat tggtataagg cagaatcact ccaatgcttt
    64921 tcgtttttcc ataacttaac tgtctggcat ttgcattagg gatataatcg acttctttca
    64981 tgaccgcaag tatttctttc cttttttcct catctacata tggatgtcca ttgattacac
    65041 gtgaaatagt agaaactgaa taacctgtga gctttgcgat atctcgaatg tttgccactg
    65101 ctgcttcgcc acctatacaa aaagattttc ttgtaaatag tctaccacag ctggaccgca
    65161 ttcaaaaaac tgatacattt ttccttcatg cgaaaaactg atccgctgcc cgccccaata
    65221 tatatctttt gtcaaatttt cgatttcatt cattggtatt tcttttacta tattcctatt
    65281 caacccttga aaactgtaca acgttaatac ggatcccttg aactcgacaa acgcataaaa
    65341 ataattgttc gttgtgccat ctgttatctc tacataagta ttcattgcaa gccctccttt
    65401 tgatactttg agtatagtgt atgaaacgcg tctcatagca aggagtttgt tttgccaaag
    65461 aaaagtttta ttcctctttc attattacca aagtctctta ttttaaattg aatacattat
    65521 aatgatactg taaatatatc tttattatta gtgaagaat
//
LOCUS       DAZUMV010000003        65434 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000003 DAZUMV010000000
VERSION     DAZUMV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.003"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1963
                     /gene="tdc"
                     /locus_tag="ACG8FK_000185"
     CDS             86..1963
                     /gene="tdc"
                     /locus_tag="ACG8FK_000185"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5095826.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            2206..3627
                     /gene="tyrP"
                     /locus_tag="ACG8FK_000186"
     CDS             2206..3627
                     /gene="tyrP"
                     /locus_tag="ACG8FK_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS5095827.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            3736..5103
                     /gene="nhaC"
                     /locus_tag="ACG8FK_000187"
     CDS             3736..5103
                     /gene="nhaC"
                     /locus_tag="ACG8FK_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5095828.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(5384..6403)
                     /locus_tag="ACG8FK_000188"
     CDS             complement(5384..6403)
                     /locus_tag="ACG8FK_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5095829.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(6425..7432)
                     /locus_tag="ACG8FK_000189"
     CDS             complement(6425..7432)
                     /locus_tag="ACG8FK_000189"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5095830.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(7425..8090)
                     /gene="pgmB"
                     /locus_tag="ACG8FK_000190"
     CDS             complement(7425..8090)
                     /gene="pgmB"
                     /locus_tag="ACG8FK_000190"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5095831.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(8083..10377)
                     /locus_tag="ACG8FK_000191"
     CDS             complement(8083..10377)
                     /locus_tag="ACG8FK_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS5095832.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            10637..12802
                     /locus_tag="ACG8FK_000192"
     CDS             10637..12802
                     /locus_tag="ACG8FK_000192"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS5095833.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            13106..13939
                     /locus_tag="ACG8FK_000193"
     CDS             13106..13939
                     /locus_tag="ACG8FK_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS5095834.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(14041..15741)
                     /locus_tag="ACG8FK_000194"
     CDS             complement(14041..15741)
                     /locus_tag="ACG8FK_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095835.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLHA
                     "
     gene            16248..17408
                     /locus_tag="ACG8FK_000195"
     CDS             16248..17408
                     /locus_tag="ACG8FK_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5095836.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            17424..17867
                     /locus_tag="ACG8FK_000196"
     CDS             17424..17867
                     /locus_tag="ACG8FK_000196"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5095837.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(18350..18880)
                     /locus_tag="ACG8FK_000197"
     CDS             complement(18350..18880)
                     /locus_tag="ACG8FK_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS5095838.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            18985..19536
                     /locus_tag="ACG8FK_000198"
     CDS             18985..19536
                     /locus_tag="ACG8FK_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5095839.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(19689..20162)
                     /locus_tag="ACG8FK_000199"
     CDS             complement(19689..20162)
                     /locus_tag="ACG8FK_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5095840.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            20300..21772
                     /locus_tag="ACG8FK_000200"
     CDS             20300..21772
                     /locus_tag="ACG8FK_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5095841.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            21769..22479
                     /locus_tag="ACG8FK_000201"
     CDS             21769..22479
                     /locus_tag="ACG8FK_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS5095842.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            22831..23115
                     /locus_tag="ACG8FK_000202"
     CDS             22831..23115
                     /locus_tag="ACG8FK_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS5095843.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(23396..23815)
                     /locus_tag="ACG8FK_000203"
     CDS             complement(23396..23815)
                     /locus_tag="ACG8FK_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS5095844.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(23817..24401)
                     /locus_tag="ACG8FK_000204"
     CDS             complement(23817..24401)
                     /locus_tag="ACG8FK_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS5095845.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(24448..25923)
                     /locus_tag="ACG8FK_000205"
     CDS             complement(24448..25923)
                     /locus_tag="ACG8FK_000205"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS5095846.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            26104..26415
                     /locus_tag="ACG8FK_000206"
     CDS             26104..26415
                     /locus_tag="ACG8FK_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095847.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(26530..28866)
                     /locus_tag="ACG8FK_000207"
     CDS             complement(26530..28866)
                     /locus_tag="ACG8FK_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS5095848.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            29008..29676
                     /locus_tag="ACG8FK_000208"
     CDS             29008..29676
                     /locus_tag="ACG8FK_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5095849.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            29679..30722
                     /locus_tag="ACG8FK_000209"
     CDS             29679..30722
                     /locus_tag="ACG8FK_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5095850.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(30959..31840)
                     /locus_tag="ACG8FK_000210"
     CDS             complement(30959..31840)
                     /locus_tag="ACG8FK_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5095851.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            31999..32727
                     /locus_tag="ACG8FK_000211"
     CDS             31999..32727
                     /locus_tag="ACG8FK_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS5095852.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32883..33200
                     /locus_tag="ACG8FK_000212"
     CDS             32883..33200
                     /locus_tag="ACG8FK_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5095853.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            33193..33801
                     /locus_tag="ACG8FK_000213"
     CDS             33193..33801
                     /locus_tag="ACG8FK_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS5095854.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            33856..34647
                     /locus_tag="ACG8FK_000214"
     CDS             33856..34647
                     /locus_tag="ACG8FK_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS5095855.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            34785..35273
                     /locus_tag="ACG8FK_000215"
     CDS             34785..35273
                     /locus_tag="ACG8FK_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS5095856.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            35324..36196
                     /locus_tag="ACG8FK_000216"
     CDS             35324..36196
                     /locus_tag="ACG8FK_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5095857.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            36372..36734
                     /locus_tag="ACG8FK_000217"
     CDS             36372..36734
                     /locus_tag="ACG8FK_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5095858.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            36743..37495
                     /locus_tag="ACG8FK_000218"
     CDS             36743..37495
                     /locus_tag="ACG8FK_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5095859.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            37509..39311
                     /locus_tag="ACG8FK_000219"
     CDS             37509..39311
                     /locus_tag="ACG8FK_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5095860.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            39588..40628
                     /locus_tag="ACG8FK_000220"
     CDS             39588..40628
                     /locus_tag="ACG8FK_000220"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5095861.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(40680..42824)
                     /locus_tag="ACG8FK_000221"
     CDS             complement(40680..42824)
                     /locus_tag="ACG8FK_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5095862.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(43181..44554)
                     /locus_tag="ACG8FK_000222"
     CDS             complement(43181..44554)
                     /locus_tag="ACG8FK_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5095863.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(44554..44949)
                     /gene="rbsD"
                     /locus_tag="ACG8FK_000223"
     CDS             complement(44554..44949)
                     /gene="rbsD"
                     /locus_tag="ACG8FK_000223"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS5095864.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(44977..45894)
                     /gene="rbsK"
                     /locus_tag="ACG8FK_000224"
     CDS             complement(44977..45894)
                     /gene="rbsK"
                     /locus_tag="ACG8FK_000224"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS5095865.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(46002..47015)
                     /locus_tag="ACG8FK_000225"
     CDS             complement(46002..47015)
                     /locus_tag="ACG8FK_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS5095866.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(47096..47695)
                     /locus_tag="ACG8FK_000226"
     CDS             complement(47096..47695)
                     /locus_tag="ACG8FK_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5095867.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            47881..48582
                     /locus_tag="ACG8FK_000227"
     CDS             47881..48582
                     /locus_tag="ACG8FK_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5095868.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            48592..51303
                     /locus_tag="ACG8FK_000228"
     CDS             48592..51303
                     /locus_tag="ACG8FK_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS5095869.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            51754..53382
                     /gene="treC"
                     /locus_tag="ACG8FK_000229"
     CDS             51754..53382
                     /gene="treC"
                     /locus_tag="ACG8FK_000229"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS5095870.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(53442..54623)
                     /locus_tag="ACG8FK_000230"
     CDS             complement(53442..54623)
                     /locus_tag="ACG8FK_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5095871.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            54875..56398
                     /locus_tag="ACG8FK_000231"
     CDS             54875..56398
                     /locus_tag="ACG8FK_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5095872.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(56467..57195)
                     /locus_tag="ACG8FK_000232"
     CDS             complement(56467..57195)
                     /locus_tag="ACG8FK_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5095873.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(57208..58488)
                     /locus_tag="ACG8FK_000233"
     CDS             complement(57208..58488)
                     /locus_tag="ACG8FK_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5095874.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(58629..59231)
                     /locus_tag="ACG8FK_000234"
     CDS             complement(58629..59231)
                     /locus_tag="ACG8FK_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5095875.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            59476..59682
                     /locus_tag="ACG8FK_000235"
     CDS             59476..59682
                     /locus_tag="ACG8FK_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095876.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            59994..60713
                     /locus_tag="ACG8FK_000236"
     CDS             59994..60713
                     /locus_tag="ACG8FK_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5095877.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(60794..61477)
                     /locus_tag="ACG8FK_000237"
     CDS             complement(60794..61477)
                     /locus_tag="ACG8FK_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS5095878.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            61695..63881
                     /locus_tag="ACG8FK_000238"
     CDS             61695..63881
                     /locus_tag="ACG8FK_000238"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS5095879.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            64227..64637
                     /locus_tag="ACG8FK_000239"
     CDS             64227..64637
                     /locus_tag="ACG8FK_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5095880.1"
                     /translation="MIKVYTSPSCTSCRKAKAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            64651..65007
                     /locus_tag="ACG8FK_000240"
     CDS             64651..65007
                     /locus_tag="ACG8FK_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5095881.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        20914 a        11619 c        13256 g        19645 t
ORIGIN      
        1 ggccaaaact tagtttacag aatagaagaa caggtgtaat gtagggctcg acaaaattta
       61 aatatttttt taggagggtt tcactatgag tgaatcattg tcgaaagatc taaacctaaa
      121 tgccctattc attggggaca aagctgagaa cggccaaatc tataaagctt tgttgaatga
      181 attggtggat gaacatttag gctggcgtca aaactacatg cctcaagaca tgcctattat
      241 tacgccagaa gaaaaaagca gtgctagttt tgaacacact gtgaataaaa caaaggatgt
      301 tctatcagaa atttcagcgc ggatgcgtac ccattccgtt ccttggcata atgccggacg
      361 ttactggggt catatgaact ctgaaacatt gatgccatct ttactagcgt acaattttgc
      421 tatgctatgg aatggtaata acgttgccta tgaatcttct cctgcaacaa gccaaatgga
      481 agaagaagta ggaatggaat ttgcaaaatt aatgagctat aaagatggct ggggccacat
      541 cgttgctgat ggttctttag caaacttaga aggactttgg tatgcccgca acatcaaatc
      601 attgccgctt gcaatgaaag aagtgacacc tgaactagtt gctggaaaaa gcgattggga
      661 attaatgaac ttgtcaacag aagaaatcat gaatctgtta gacagcgtcc cagaaaaaat
      721 cgacgaaatc aaagctcact cagcacgtag cggaaaacat ttagaaaaat taggaaaatg
      781 gttggttcca caaactaaac actattcatg gctaaaagct gccgacatca tcggtatcgg
      841 tttagaccaa gtgattccag tgccagttga ccacaactat cgcatggaca tcaacgaact
      901 tgaaaaaatc gttcgtggtc tagcagctga aaaaacacca atcctaggtg tagtcggtgt
      961 tgtaggatca acagaagaag gagcaatcga cggtatcgat aaaatcgttg cattgcgtcg
     1021 cgtattagaa aaagacggta tttacttcta cttacacgta gatgctgctt atggcggata
     1081 cggccgtgcg attttcttag acgaagacaa caacttcatt ccatttgaag atttaaaaga
     1141 tgttcattac aaatacaatg tctttacaga aaacaaagat tatatcttag aagaagtaca
     1201 cagtgcatat aaagcaatcg aagaagcaga atctgtaacg attgacccac ataaaatggg
     1261 ctatgttcca tattctgccg gtggtatcgt catcaaagat atccgcatga gagacgttat
     1321 ctcttacttt gcgacatatg tatttgaaaa aggcgcagat atcccagcat tacttggggc
     1381 ttatatcttg gaaggttcaa aagcaggggc aacagcagcc agcgtttggg cagcacacca
     1441 tgtcttacct ttgaatgtca caggatatgg taaattaatg ggtgcatcaa tcgaaggtgc
     1501 acatcgtttc tacaacttct tgaatgactt gtcatttaaa gtaggagaca aagaaatcga
     1561 agttcatcca ttaacttatc cagatttcaa tatggtagac tatgtattca aagaaaaagg
     1621 caacgatgac ttagttgcaa tgaataaatt gaaccatgac gtatatgatt attcttcata
     1681 cgtgaaagga agcatttacg gtaatgagtt cttaacttca catactgact ttgctatccc
     1741 agactatggc aacagcccat tacaatttgt gaaccaacta ggattctcag atgaagaatg
     1801 gaaccgtgcc ggaaaagtaa ctgtattgcg tgccagcgta atgacaccat acatgaacaa
     1861 agaagaacac tttgaagaat atgctgaaaa aatcaaagca gctcttcaag aaaaattgga
     1921 aaaaatctac gcagaccaat tattggcaag cgaagcaaaa taagcaaggt aatcatgtta
     1981 gaaaacagct tgaatactaa ctgtttctga cgcatgtttg gatctgaaga gcaaggggag
     2041 acctaagctc ttcgatccct ttgttttgag gttgtctttg aagaaaccct tcaagcaata
     2101 aactcaaaga atcccctcaa atattcgctg aattgtttcg tttattgctg aaactacttc
     2161 aaagacaccg ttattttatc caaaaaatca aggagagtgt caacggtgag taaatctgaa
     2221 gtaagtaaaa tgggtcttgg gacctttatc ggtctgacaa tggcactgtg tgcaacggtt
     2281 cggagtattc ctacgttagc cgcagtcggt tggacattga ttttttattc aatttttgca
     2341 gttttgtttt ttgccggtcc gatatcaatg gtttccggtg agctttccac gatgcttcct
     2401 caagaaggcg gtcctcagtt atgggtaaaa acagcactgg gcagcaaatg gggatttgtc
     2461 gttgcatggc tattatgggt gcaaatgttc ccaggtatgg taatggtcgc ttctacttta
     2521 ggaccattgt taggaaacac atttggtaat gtagaacttg gcaataatca ctggttcgtt
     2581 ttaggttgta ttttagttat ctactggatc atcacgatct tgaacttgaa attcgatatg
     2641 gcaaaagtcg gcgggaacat cggtgtatgg ctaggggtat atatcccagt agtcatcatg
     2701 ttcgttttag gtgttttggc agccttcaaa gtcggactag tctcaaacgg ctatctaggt
     2761 gacttttcat ggtcaaaagc tttccctgac ttggaacata ttgattcatt gaaatactta
     2821 gctggtatca catttatttt tgttgggatc gaaatgagtt ctgtttacat gcctcgtttg
     2881 aaagacgcaa caaaaaatta tacaaaaggc gtatttatcg ctttgatcgg attagtttta
     2941 ttgaatgtca tcaatgcgat gcttgtagca aatgtcgtac cagatggaaa aatggaatta
     3001 gcaaatatca cgcagcctat cttgattgac tgtcaaatcc taggcttacc agaagtgatc
     3061 ggaaatatct tcagcttcat ggtatttatt ggtgtgcttt tacaattatc agcttgggtt
     3121 actggaccat caaaaacaat catccaagta gctcgtgaag gattccttcc accaaaattc
     3181 ggattccata aagaaaacaa atacggcgta tctagaaatg tcgttctaac acaatcaatc
     3241 gttatttcat tatttgcttt gctttatggt gtaatggatg acgtaagtgc tgtattcttg
     3301 acacttacaa atgctacaac tgttatttat tgtatcgtgt acattttgat tgctgtttca
     3361 ttgcttaaaa tgcgtaagaa acatccagaa ttcgaacgtc cttaccgaat tggtaaaaac
     3421 ggaaacggat tagcttgggt agtcagctgc atgctgatct tctctatcat cgttgtcgta
     3481 tttgctacat taggtacagc gacattgagt gatgcattat tagtagcagc tatcacagtg
     3541 gtcatgttcg ttattccatt gatcatcaat cacttcaaga aagattcttg ggggattgag
     3601 gtagaaaaaa gtttagaaga aaaataaaaa aataagtctt tcagccgacg ttttgtcgct
     3661 gatttagtag gaggtcctgc caagtgaata gttttttcat ggcagggcct tttcatttga
     3721 aataggagat cgacgatgaa taaaaatatt tctttaaaag aaagtatcgc tgtattgatc
     3781 gtgttgttgg cactcctagg agtactgatc atcggattcc agctctcgcc acatatcccg
     3841 atccttgcag ctttcatgtg tttgttgttt tatggcagat ttaaaaagtt cacatgggat
     3901 gagatccatg acgggatcat caaaggaatc actccaggga tcattccaat cctgattttt
     3961 cttttgattg gtgtcttagt tgcttcatgg attttatctg gaaccatccc tacgattatg
     4021 gtttatggat ttaaacttgt ttctgtgcgt ttctttttac cgacagcatt tcttgtttgt
     4081 gcaattgtcg gagtgactgt cggcagttca tttacaacga tttcgacgat tggaattgct
     4141 tttttgggga tcggtcattt acttggattc aatgatgcaa tgactacagg cgcagtcgtt
     4201 tccggtgcct ttttagggaa taactgctcg cctttatcag atacgaccaa cttggcagcg
     4261 ggtattggag gagttaatct attcgaacat atcgctggta tgcgttatac ggatttccca
     4321 gcattcgtcc tttcgttgat tttttatacg atattaggac ataacagtca ttcagccgat
     4381 ctagctagta tcaatgaaat gatccaaaac atgaaagcga gcttttggat ctctccttgg
     4441 acattgattc cactagttat tttatttggg ggggcaatca aaaaaattcc ggctatccct
     4501 acattactag cgggatcgac agctgctctc gttctttctt ttatccatga gaaaagtttg
     4561 acggtcggaa aagcagcaga catcttgatg aatggttatg tcgcaaatac acctgatcca
     4621 aagctagatg aactgctttc tcgaggcggg atcatgagta tgttaggttc tgctagtttg
     4681 attttacttg cattggcatt aggtgggtta ctgatcaaat atctgatcgt tgataccatc
     4741 gttcaagaat taaaagaaaa gatggaccga ccttctcgtt tagtcgggtt tactgccttg
     4801 agctgtatcg gaatcaatct gatcgttgga gaacagtatc tgtccatcat tcttccagga
     4861 gaaacattca agcgttcttt tgatgacgtt ggcttatcaa aaacctattt gacacgaaca
     4921 ctagcggatt caggtgcgac cgtcaattct ctcgtaccgt ggggagtcag cggcacattc
     4981 attatgggaa cgatgaaagt tagcgcgttg cattacttgc cattcgcctt tttctcgatt
     5041 cttgcacctg tctttacaat attaggcggt tttttattga atcgtcgacg tgtaaaaaat
     5101 tgaaggaagg aaaaaagagg ctgggacaga agcgtttaac tccaagaaat aagaagaaac
     5161 tcaagaaaat tgctttttaa atttttgtga atttcagctt atttccgaag gagttgcttc
     5221 tgcttccgcc gtttatacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc
     5281 acgctctttt ttacagataa acaatgtgtt agaagtagta tcttctttta cataaaaagc
     5341 tgaccatagg ataaatggtc agcgtattta gtgagtcagc tatttagtat gggttttcta
     5401 aaggaacgac tgtctctctt ttgatcaagc gatgagggat gaccaaacgt acgccactgc
     5461 tcaattcttc atggatcaat tcgcgcagct tttgcatagc gacacgtcct aattcggaga
     5521 tatcgatatc gatacttgta aggtaagggt gtgttaatgt tgagaagatc gagttgttga
     5581 agctaatgac ggagagtgtc tctggaatga catagccata catttgcgcc aactgcatcg
     5641 tgcgaagggc gaagatatcg tcaatgacca ccaatgctgt ggcttcgttt tctttcaacg
     5701 ttttctcaaa ggacagataa tcctccggat gctctagtac tacactggat aacgcaggaa
     5761 gaccagccat catcattgct ttttgataac cgaaatatcg ttcgaaatac aaactttcat
     5821 gagtcgtgtt tgtcacaaac aggatattct tatgtccatt ctggatcaaa aattctgttg
     5881 cttgtttacc tagaagctgg ttatcattat ctacataaac gatttcgttc tcatgatgat
     5941 aaggctgtcc gatcaaagtg aaagggatgt ggttttggta taaataatca atgacagtat
     6001 cttgactatc tgaatacact agaataaaac catctacttg tttttgtaaa tgcatacgct
     6061 ggacgttctc taacagcaat tcaaaatttt tagcagtagc gattgctgta gtcatatcat
     6121 aattccgcgc ttcttcgttg attgcttcca tgatctcaag ataaaatggg ttaccgatcc
     6181 gctcttttga atcaagaggg ggtaagatta cgccgatggc actggaaatg cgttttccta
     6241 gattgcgggc agtcatgtta ggaacataac ctagttcatc cataattttt cggactttct
     6301 tctttgttgt ctcggaaata ctaggatgat cgttgatgac acgagagacg gtcgaggtgg
     6361 caacaccggc ttttttcgcc acatctttta cggtaatagc catgataaaa acacctttct
     6421 cttgttattg tgcagagaac cgataaatcg tctgactgat ccgttcttga ccaggatgga
     6481 ggttgatcga tccaaatttg gggaaatgaa cgatatcagg gatttcttgc ggttcgattg
     6541 ctaatccgta gtttgaatgc atctgttttc cattgacaga gaaatcagct tcgaatccag
     6601 tcgtagaaaa taataccata cttttattag aagttgctat agtcatttgg cgaccagaat
     6661 gcttctcgaa taattgtaag gaagttttgg aaagcctagg atgatgcaaa agaaatacat
     6721 catctagtcc atctggataa catgcaagga tctcttgcag tgttggggat tttctaaagt
     6781 cgattggcag tttttctttt tttgtccaat ttccagtagg cagtttttct tgatctaatt
     6841 caagcacacc atctaaaaag accgttaact gatgttcata gatgtctctt gttccatttc
     6901 ctgaaaggtt gaagtaggca tggttggtcg gattgcagat ggtttcaaca aggctactag
     6961 cagaggttgt catctctaat tcgttttttg ttaaacggta agtgatcgta gcagtgattg
     7021 gtcctggata ggtagaaaat tcatctttta agtaaaagac cacacctatt gatgatggag
     7081 ttttgaaaac ttctacttcc caaaattgga aagaccagcc tttcgtgcca ccgtggatat
     7141 ggtgtgatcc gcagtttttc tccaatctat gctcacccca agaagcatcc cggatccttc
     7201 cggctacagg tccgacagtt gctccaaaaa agcttttgtc tgttagaaca tcttcgaaac
     7261 gatcgtaaga caataaaaca ttttctgatt ttccttcttt gtctggtgcg aagatttggt
     7321 gcaggcgggc accgtaattg agaagaacga cttctaagta gtcgttcttc aatgtgattt
     7381 tctttatttc agtctctgga actggtacga cggagatctc tggattatcc atggtcatgc
     7441 cacttttctt tcaagtaatc gatcgtaaga aaagtcgtat cagggacaag cgggatctcc
     7501 tctccaaggt cttcagcacg tcccacgcca attggttgag cgccacttga gaggatggct
     7561 tggataccgg cttgcgcgtc ttcgatcccg atacattctt ctgcttttag tccgacagct
     7621 tttgctgcaa ggaggaagat atctggcgca ggttttccgt ttgttgcttc tgccgggtcg
     7681 gcaatagcat caaagtaagg tgtaagaccc attttttcaa gtaggaatgg cccgttttta
     7741 cttgcagaag caagagcaat cttgattttt tcttctttta aagaatccag taaaggcacg
     7801 accccgggga agacatcttt tggttcgatc gtctggatca tctctaaata ataatcgttt
     7861 tttcttttcg ctaattctgc aaattcttcc tttgaaaaag agtgttcttt tccgccataa
     7921 gctaaaagca aagagagtga gtcttctcgg ctgacgcctt ttagctgttc gttgaatgtg
     7981 tggtcgatcg taatgccgat ttcctgacca agctttcgcc aagcacgata atggaattca
     8041 gcagtatcgg taatgacacc gtctaaatca aacaataccc ctttaaacat ttgcttcctc
     8101 ctttcttagc gttatttcta gacgatccgc taaagaataa gactgttcat agacgtaaat
     8161 ttctagagga gaaccttcta ataactcgaa agtaacttgt tcatctgtga ctgagatgaa
     8221 caataaacgt ccgcgataat tgatatggaa agaataagct ttccaagttt ttggcagaaa
     8281 tggtgcgaat ctcaactgat catgggcagt tttcatttga gcaaatcctt ggacaatcga
     8341 caaccagcta ccagtcatgg aagtgatgtg caagccgtct tctgtgtcgt tattgtaatt
     8401 atccaaatcc aagcgagctg ttcgctggta catttctaca gctttgcttt ccattcctaa
     8461 ttctgctgct aaaatcgaat ggatacttgg cgaaagggaa gactcatgga cagtcattgg
     8521 ttcatagaaa tcaaagtttt tttgcttttc ttcttttgta aaagtatcat tgaagaaata
     8581 aatcccctgt agtacatctg cctgcttgat gaagcaagat ctaaggattt tgtcccaaga
     8641 ccaatgctga ttcagaggaa gatcgcttgt gggaatatcg gatacaggca tgagatcttt
     8701 atctaagaat gtgtcatgct ggacaaagat tcctaattct tcgtcaactg ggaagtacat
     8761 gttctctgcg atatctttcc atttggctag ttcttcttcg ctgatagtga tagcagcttc
     8821 tttttgataa gttaaataat tttctcgcgt gtaacgcaag acccaagctg cgatcgtatt
     8881 tgtataccaa ttgttattga tattgttctc atattcgttt gggccggtca caccgtggat
     8941 catgtattta tttgctcgtt ttgaaaaatg gacacggtct gcccagaagc gtgcgatttc
     9001 tgctaaaact tccagtcctt catttttcaa atattccgta tcgccggtgt aattcgtata
     9061 attgtagatt gcataggcaa tcgcgccatt tcggtgaatc tcctcgaatg tgatttccca
     9121 ctcgttatgg cattcgacac cagtgaaggt gaccatagga taaagcgcgc cttttaatcc
     9181 ttgttgctga gcgttgtgga ttgcttgcgg cagttgatta tgccgatatt tcagcagatt
     9241 cttagttact tcaggtttgg ctaaagcaag ataaagaggg acagcatacg cttctgtatc
     9301 ccagtaagtg gctccgccgt atttttctcc tgtgaatcct ttggggccga tattcaatcg
     9361 ttcatcttcg ccgtagtaag tagagaatag ttggaacaaa ttgaatcgaa taccttgttg
     9421 tgcttcttcg tctccttcga tgaccacatc ggcaagcttc catcgttctg cccaagccgc
     9481 tgtttgctct tcttttgctt ggatataatt gtttgtctgg ttcgttaatt ctgagacagc
     9541 tttttctgct tgctgcgctt ctggaatatc tctactagta agaacgataa tttctttttc
     9601 cactgaccat gtctcacctt gagataattc tgcagacaag caaccatgta atgctaaagt
     9661 atcttcgtga tagtcaggga tgactgattt tccattaacg aaatggcgca ttgctgcagt
     9721 aacagtaaat tgttcaatcc caaagttgtt tggaatagtt ttagtcgtca aatagccgat
     9781 ttcttctata gttccacgat caatctcatt ccaaaaatgt tcatcatagt tgctgtcttc
     9841 gttttgaaca tttccatcca attttgatac gagtttgact gttccgctac cttcaagcat
     9901 ttctatagtt aaatagatat aagctgattc ttttttgaca atgctcagaa aacgttcgaa
     9961 ggaaatgcgt actttattag tagaagtagt gattgtgaat gtacgagata atacaccatt
    10021 tttcatatct aattcccagt aaaagtcttc tggttccaaa gaagccaagt cgatcagctg
    10081 atcgttgatc gaaatatcta tcgcgataaa gttcaaggcg ttgatcactt ttccaaagta
    10141 ttctggataa ccatttttcc accagccgac acgtgtctta tcgggatacc agacacccgc
    10201 caaataagta ccttgatggt ggtcccctga gtaagtttct tcaaagttcc cgcgcatccc
    10261 catataaccg tttccaatac ttgttaaaga ttcttgcagt cgaagatttt ttttatctaa
    10321 ttgtgtcgtg ctgattttcc atggatcaat agcaaatagt cgtttgatct gtttcatttt
    10381 tctcctccta atgagctgtt ttatacgttc ataataaacg caaccgattg cacaagtcaa
    10441 tagacaaaag aaaatatttc atagaaaaaa gcttaaaaaa ataaattttt aggagaaaat
    10501 tatagaaggg aacacacgta ttataggttt tatagaatgg tatagacaga aaaataaaaa
    10561 ataaagcatt tacaaaaaag aaaacgcacg ctataatacg tttaacgaaa ccgattgcgt
    10621 aaaggagcta aacggaatga aaaagatatt tagttttgag ttttggcaaa aatttggaaa
    10681 ggctttaatg gttgtggtag cagttatgcc ggcagctggg ttgatgatca gtatagggaa
    10741 atcaattccc ttgatcaatc ctgattggac gccgcttgtc acaacgggcg gagtaattga
    10801 aaatattggt tgggcaatta ttggtaatct tcatatttta tttgctttag caataggagg
    10861 aagttgggca aaagaaagag caggcggagc atttgcagct gggatctcat ttattttaat
    10921 caatcggatc actggggcta tctttggtgt cacaagtgac atgcttgcga atgaagatgc
    10981 atttacccac actctgttcg gtacgaagat catggttaaa ggatttttta caagtgtatt
    11041 ggaagcacca gctttgaata tgggagtatt cgtagggatc atcgctggat ttgtcggggc
    11101 aatggcttat aataagtact ataattatag aaaattgccg gatgctttgt catttttcaa
    11161 tggaaagcgc tttgttccgt ttgttgttat cttatggtca acaatcgtgg cattgattct
    11221 tgctgtggtg tggccaaacg tacaggctgg aatcaataat tttggtttat ggattgcaga
    11281 atcacaagaa agcgcaccga ttctagcacc gtttttattt ggaacattag aacgtttact
    11341 attgccattt ggattgcatc atatgctgac gattcctatc aactatacac agcttggcgg
    11401 atcgtatgag attttatcag gcgcacaggc ggggacacaa gtatttgggc aagatccgtt
    11461 atggttggct tgggccactg atttagtgaa tcttaaaggt gcaggggata tgtcgcaata
    11521 tgagtttgtg ttgcataact ggacgccggc tcgtttcaaa gtaggacaaa tgattggttc
    11581 ttcaggtatc ttgatgggaa tggcttttgc gatgtatcgt aatgtcgatc ctgataagaa
    11641 agcaagatat aaatcaatgt atttttcagc agcgttggct gtctttctaa caggtgtaac
    11701 agaaccattg gagttcatgt ttatgtttgc tgctgtgcca ttatatgtca tttattcagt
    11761 gatccaaggg gctgcatttg cgatggcaga tatcttgcca ttgcgggtcc attcatttgg
    11821 gaatatcgaa ttgctgactc ggacaccgtt agctatcaaa gcgggtctag gtggagatct
    11881 tatcaacttc gtgttgtgtg tgatcgcttt tggtgttgtt acgtatttct tagcaaattt
    11941 cttgatcaaa aaattcaact ttgctacacc gggacgtaac ggaaattacg acaatgacag
    12001 tgaagaaacc gttgtatcta acagcggaac aggaacagca gatcagcaag tggtccagat
    12061 cattcatttg ctgggaggaa aagaaaatat caaagatgtc gatgcttgta tgactcggtt
    12121 acgcgtaagt gtaaatgatc gagaaaaagt agggactgaa gaagcctgga aacgagcagg
    12181 ggcgatggga ttgatcgtca aagacaatgg agtccaagca gtttatggac caaaagcaga
    12241 tgtcttgaaa tcagatatcg aagatctgct gcaatcagga gcagagattc cagagccaga
    12301 aatggtttct acaggaaatg gacaagcagg tagtaagcaa tacttaggta tagaacaaga
    12361 actagtttcc gcagcttctg gagaagtgat tcctttatct gaagtaaaag atccggtatt
    12421 ctcacaaaaa atgatgggtg acggctttgc tgttattcca acatcaagag aagttgttgc
    12481 gccgatcgct ggaaaagtga caagtatttt ccctagcaaa catgcaattg ggctggaaac
    12541 gaaagatggt atcgaagttt tgatccatat gggaatcgat acagtccaaa tgaatcagcc
    12601 agcttttgag attgctgtca aagaaggcca agaagtagga acaggaacga aattagctga
    12661 gatggatctt gatgtgatcc aaaacgaaga aaaagatcca acgatcatga tcgtatttac
    12721 aaacgataaa gtagaagaag tagtgatcaa gcaattaggt acagtgacag ctggtaaagt
    12781 gatcggctcc attaaaatgt aaggagaggt tgtgaaaaag ctgtcctgca tcaagtcata
    12841 agaacagcca aacaaaaata gctcctccta tttttcgttt ggttgggctt attaccgaga
    12901 agcagcagtt tgaatactgt ttagtggaga ggtcgtgaaa aagctgccct gcaccaagtg
    12961 ataaaaacag ccaaacaaaa aatagctcct ggtccattga gcttattact gaggagttag
    13021 caggagaaca ctatttgctg gagaggttgt gacaaaaatt ttgtcacaac ccttttgttt
    13081 cgaaatgaag acaaggagaa aaccaatgaa attcatgaca ctcaacacgc atagctggct
    13141 tgagccagaa ccagagaaaa aattgcaaga attagcagat aagatcctgt tagaagatta
    13201 tgaagtgatt gctttacaag aaatcaacca attactggaa tctgaagaag tagaacctgc
    13261 gaagctaatg aagttctgtc cggtaaagaa tcaagtacct atccgtaaag ataattttgc
    13321 ctaccgttta gttcagcttt taaaggaaca cggaaaagag tatttttgga gttgggaaat
    13381 gagccacatc ggttatgata aatatgaaga aggaaatgca ctgcttacga aaaaaacatt
    13441 ggaaagtcag gtccttaccg tctcccaatt tcaaaagaaa gaagattatc agacacgcaa
    13501 gatcttgatt gggaaaacaa agatagatga tcaggacatt tttgtctcga gctgccactt
    13561 ttcttggtgg acagataaaa aaagtggctt ttattttgag tggaaaaact tggaaaacta
    13621 ctttttggaa actcgcgtac cacttttttt cctaggtgat ttcaataatc cagtagactc
    13681 tcaaggttat tatactgtaa gagaaagttg ccttcttctt caagattcgt atgttgtcgc
    13741 gaatgaaaaa ggaaaagcag ctactgtgga aaagaaaata gatggttggg aacaaaatac
    13801 agaaaaattg cgtatagatt ttattttcgt ccctgaagga atgcaggtta aaaaatatca
    13861 acggattttt gatgggatcg atagtccaat catcagtgat cactacgggg tggaaatcga
    13921 attagatgtg aacgaataaa aaatataaca tataacataa aaaaactgat taaaataaaa
    13981 ctctccaaac gattataccg tttggagagt tttttgatcg gatgtattta tcccttttgc
    14041 ctaagcatgt aaaagcttgt atctatgata aataaataaa catagcaagg taaaccagcc
    14101 aatcaaagta agagaaagaa tgaaatagaa atagattggc gtttgataat gaagcgtgag
    14161 ctcgttgatt cctttttgct gcttcacgat tggggtcccg atatctgtta cttcataatc
    14221 agtcaatgtt tttccatttt gctgtagcac agtccgatcg tagacgataa cagggatatg
    14281 aacagttttg tccccagtgt ttttccactt gattgtcaaa agaccgcctg gatgtgtttt
    14341 tataaaagtt gtttcgtttt ctagtatttc ttcgttatac cggtcgtatt tattgtcttt
    14401 tgtctcctta tagattggca gatagtcagg tgtggttttt tggaaaacga acaagctggt
    14461 agacatatct ttgctgaaga atgttttcct tgcttcttcc ggcgtatcga atagataagt
    14521 atgtcgactg acaaatgttt cattttccca cttttccaga tgctggtata agttctggat
    14581 cgtctgtgct gaaaaaataa gaatgccaaa tatacttaaa agacggaacc acttttgttc
    14641 agaccactgg ttcagtgaaa gggcaaggta aagtagaagc aatagcgtaa atggcacgaa
    14701 aaatcgaaat ggaaactgga tcaaactaat aaaaggcaca tttttttcga tcaatgtcgt
    14761 ccatggaatc aagctagtag aaagaaaaaa gaaaacaaga cttgtgtaca acacgatttt
    14821 ttggaataag gtgttctttt gttgaaagaa aataaaaagc aaaaaacctg ataatatcaa
    14881 tataggaaag gccttagggt aatggagcca ataactgcca tccagatcta tcgccttttg
    14941 actcatttct ctgttaacaa agggaggaag aatcttattc ccactgtaaa caatagctaa
    15001 tcctgcccaa atatttgctg tcagaacaaa ataaagcaag accgacttaa tcagttgaag
    15061 cagtgctttt cctttttcag tctggttaaa aaagtaataa agataaaaag ggatatacat
    15121 caaagctaac atgatcgaag acagtaaatg gatctgtgtc ataagtccca ctgagacagc
    15181 gactttgata acagggaact ttttatgaag gacgaaatca gcaataggga tcatacataa
    15241 aggataaaaa caagtccccc agctagtgaa tccttggttg atagaccagt attgaataga
    15301 atacgtactc atgaataaaa cggctactgg tacacttacc caatttcgaa cattgacata
    15361 tctcagtaaa atcagcatag aaaatcctga aataagataa agaatgaaat ttgaaagaat
    15421 ctgataatga aaccagctac ctgcaagcaa cacgatcaat ccttgtaaat aagcgaaaaa
    15481 tgggccgtaa acggcattca cgatcctgcc actttgctgg aatccataaa gggaaggaaa
    15541 ataatggaaa tccccatatt taatttgttg cgcggcatca tagaaccgat tgtaatggaa
    15601 tggggcatca gcacccaaaa ttagtttatg tgtgaataaa tgaggcatga gaaatgccaa
    15661 agcaatgaaa aaaattgcta aaaatgggcc atattttttc aattgtcgaa ctcctttgca
    15721 aactttagat aaattcatca tatatcctat gcgagaggta ggcaagaaat tagaaaattc
    15781 atgaaaatat ttatgaattt tacaagaaaa acataagtga aagttctaag gagagaggat
    15841 gcgacataag ttttgttgca tctccttgtt ttgaaacaga atttttctta atactcgaaa
    15901 caaagtagct ttttcatgac ctctcggtaa acggtgttca atcagctgct tctcggtaat
    15961 aagctcacta gcctagaaaa tatgagaagc tattttctga ctagcgctct tattactcaa
    16021 agcataacgc agttttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg
    16081 taataagccc aacccaacaa aaaatatgag tagctatttt tttgggctgt acttattact
    16141 tgatgcagga cagctttttc acaacctcat cggctttcat tttctatttt tttcctgaac
    16201 ccatataata aaagaaagaa caaaaaaact tgcaagggag gacacatgtg aagcagagta
    16261 agcaaaaaca gtctcgtcga cttttgtttc gtttgttttt attaatatgt atcgtcgtag
    16321 ggttactgat cgctttatat ccattttatg tggattcttt aaatagtttt attgatcaaa
    16381 aacgtatcga acaagtacaa aaacggaccg cagctgaaat tgaagtgcag cgaaagaaaa
    16441 tggaggagaa aaaccaacga ttaactgacc aaggattcaa tcctggtact gatcctttca
    16501 atgaaaagaa cagaaatgaa agtacaacat ccagccaatt agaggaatgg ctgatcggaa
    16561 gtatcaatat acctaagatc caattaaata tttcacttta tgatcggctc aatggtagga
    16621 tccttgaaaa tggagcaggc gtcttgcaag gaacctcttt cccgcttggt ggaaattcga
    16681 cgcattcagt gatctctgct cacagcggtt tacctaatcg acggttattt accgaattag
    16741 atcgtttaga aaacggagac acgtttattt tgactgtttt gggggagaaa ttggcttatc
    16801 aagttaaaaa tatacaagtg gtactgccag atgacacgtc tgttctaacg atttcagaag
    16861 gagaagactt agttaccttg cttacatgta cgccgtatat gatcaacacg catcgtttgc
    16921 tagtgacagg acatcggatt ccttataacg aatcggtaaa aaaagaagaa ggaaaaggaa
    16981 atcaggaaag aaccatgcgg caatggttta ttcttgcagg aacgataata gctgtagtca
    17041 tactgttagt gtttatcgga cgactaatct atcagtatag attaagtaaa aaagtgcttg
    17101 atttttcatt tattattagt gattcgtcag ggaaaccggt aaatggtagt tcattcattt
    17161 tgaaacataa gaaaaaaaca cttactagaa atggtgtgcc gttttcagta caaagcgacc
    17221 atcatgggaa agtcaagctg gatcaattgc ctggtggaac ttatcgaatc gtttcagctg
    17281 atgatcctaa agttgctgtt tcttttggta ttagaaaagt aaaacaagag aagatatact
    17341 tttttgaggg gcgaaaactt gtaaaagagc ttcaaaaaaa tggtttttat tttaaaatca
    17401 atgaataagt aaggagagaa aaaatgatcc gcacaacaac aaaattgtct gccaaagaac
    17461 tggaatctgt tctatcgatt tggctgcatg ctaacttgga ttctcatgaa ttcattccat
    17521 caacttattg ggagaatcaa gtagaatctg ttaggaaagc tttaccagat gcagtgattt
    17581 acttagcaga aatagatcaa gaaattattg gttttgctgg ggtgattgat cagtttatcg
    17641 caggaatctt tgtgaaaagg gaataccggc aacaaagaat cggtacgcaa ctaatgaaaa
    17701 aaataaaaga aaccaataaa acactgactt tgacagtcta tcaaaaaaat caatcagcta
    17761 ttcatttcta tctttcacaa ggttttacaa ttcaaacaca gcaaaaagat gaagcagtaa
    17821 atgaggagga acttgtgatg aaatggaatc aaaacgctga tttttgatga acagaataac
    17881 gaaataatgc tcccagccta attttttgct gggagcatta tttcattttg aagtcactaa
    17941 tttaactgtg tgaatatgca gttgttacaa tctttgataa acggtgttca atcagctgac
    18001 cctcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccat ttgtttctct
    18061 aatactcagg tcaaaacgct gatttcacaa cctcttgata aacggtgttc aaacggctgc
    18121 ttctcggtaa taagcccact agcctagaaa atatgaaaag ctattttcta actagcgttc
    18181 ttattactca aagcataacg ctgatttcac aacctcttga taaacggtgt tcaatcagct
    18241 gaccctcggt attagctcaa aaaacggcaa aacatgagga actatttttc cgtttctttc
    18301 tctaatactc aggtcagaac gctgatttca caacctctct ttcacaacct tattttactc
    18361 gtttataatt ttcatcgaag tattttccgc tacggatatc atctggcatt ccatcgtatg
    18421 gttcttcgct aataacatct tcattgtcta tacctgcatc acccatataa gtgatcgtag
    18481 caaattcagg aacaagtaat tttggataag tttcatattt ttcacgagct gcttccattt
    18541 ctgcgatatg ttcgttttgg aaagtaacag tgatttctgg atgttcttca acccatttag
    18601 ggaaaaagat cattccgtga agtttctttt catttggcat aaagtccaat gccacgtctg
    18661 tccctgttgg ctctgtccaa gtgattttga atacgccgtc cgtcaattta acgatatcag
    18721 cttcttggtc ttttacccaa cggcctttga ccattccgcc atggatacgg taatcaacag
    18781 tatggtcatt tttggcatac cattcatatt cccaaccatt gtcatacgta tagataaagt
    18841 gagttccaat aaaatcttcc aattctttaa attctttcat gtttaagcat ctccttataa
    18901 attaaaacat gtaataaact acatgttttc gattactcat ttatgataga atggatttaa
    18961 agatttgtca aggagggaaa actgatggca agaaaaaata cacttcgata tattttgtta
    19021 gggttattaa gtaagaagaa gatgacagga tatgaattga atcaatcgtt caaaaatgaa
    19081 ataggagaat tttggcaagc aaaacatagt cagatttatc ctgaacttgc aaaaatggaa
    19141 gagcaaggaa tcattcagca tcaagtagag atcacaggtg aaaaattgga aaagaaagtt
    19201 tatgaattaa cagaagaagg gaaggaacaa ttggcagagt ggattcatac acccaccaat
    19261 gaactaccgg ttaatcgaga cgaatttgtt ctgaaattgt atttcgtcaa agacgtgaat
    19321 gatccggcat tagtagaaat tattcagcag cagcgtgacc ttcatgaaga aaaacgactt
    19381 catctattag agcgaaaaaa aacgatcttt cctactgaaa aagaaaaaca tgaaaattac
    19441 ggacactacc tgattttagc gcatgcgatc aatcgggaaa cagaatatgc aaattggcta
    19501 acagatgtat tagaagaaag taaccgaaaa aaataaaaaa atatccgaac agtgatggag
    19561 aattcctttc tatatgaaag aaacgcagat caccgttcgg atattcttat gttcaaacag
    19621 gccttttttc aagtatttat gtagcatcta cttattaaaa agcttggtac ttaggagatt
    19681 agtggcgttt agagttcttt taacaactgt tcgtagtcag ctttttcatc gacaggaagg
    19741 agcttgtaag ttaattcgtc tgtctcgttg tcatgaatga tgtacatctt agcattctct
    19801 tctttgaagt taccgatttg taaaatcgta tagcgttcgt cgatcaattc gatatctttg
    19861 attgcttttg aaaaaacttt acggacatgg tgcattttct tttggcggat ttctgccttt
    19921 tctgcagctt ttgacaaagt aaatccgtca tctaaatatt ttttgatcag ttctactttt
    19981 atgactgtag gtaaacgata ttttctagga caattcgcat cagcttcgat cgaacgaatg
    20041 aatccctttt gttcccaata ccttaattga cgtggagaca cacctacgat atcacttaat
    20101 tcactgatgc caacaaccaa ttgatcattt tccaacaaac gtttaaggga agccccaacc
    20161 atttttgtca ctccttttgt cctcttgctt cccttaatta tatccgtatt aacttatgtg
    20221 tcaagtaggt tgacaaatag cgaatttagg actaaagtat tctagtagtt tcagaaaaaa
    20281 ttcagaaaga aggagaaata tgagtaacaa ccaacccgta gatatttacg gaaaaccata
    20341 taatcggaca cttttagttg ttgtcttatt aattggtaca ttttgtaccg ttttgaatca
    20401 gaccttattg acgacagctt ttcctacatt gatgaaagct ttcgatattt cagcgtctga
    20461 tgtgcaatgg ttaacgactg gtttcttgct agtcaacggg atcatgatcc caataacagc
    20521 atggctgatc aataaattca gttcaagaaa tttatatctt tctgccatga cgatcttttt
    20581 gatcggaacg attacttgct ttacagcacc taattttagt acattactaa tcggtcgttt
    20641 gatccaagct tgcggtgtgg gagtatcgat gccattattg caaaatatca tgctatctat
    20701 tttcccgcca gaaaaaagag gatcagcaat ggggatgtca gggatcgtta ttggacttgc
    20761 tcctgcttta ggccctacat tatctggtta tatcattgat aactaccatt ggcgtgactt
    20821 gtttggtatg gtactgccaa tcgttgttct tgtcttggtt ttagcattct tcctaatgaa
    20881 aagtgttatt ccattgtcaa atcctagcat tgatatttta tccgctattt tatcaacgat
    20941 tggattcggt agtttacttt acggattttc tagtgtagga gatgacggct ggggaagctt
    21001 gaaagtaatt ggtttcttag ttgttggtgt gattttcatc gccttgttct catggcgtca
    21061 attacatttg gaacatccat tcttggaact tcgtgtattc aaatcatcta cattcacgat
    21121 tgcagctgtt ttagctggtg taacaaatat ggcgatggta ggggcagaaa tggtcgtacc
    21181 attgtatatt caaaatattc gtggagaatc agcattccat tctggcttga tgttgttgcc
    21241 gggtgcattg atcatggggg ctatgatgcc tgtgacgggt gcgattttcg ataaatacgg
    21301 cgcgaaacgt ttagccatca cagggatgtt cattttgaca gcggcaactg caccatttgc
    21361 tttcttgaca aaaacgactc cagttttata tatcgtgatc ctttatgcga tccgtatgtt
    21421 tgggatctcc atggtcatga tgccggtaac gacttctggt atgaatgcat tgccaaataa
    21481 cttgatcagt catgggacag cggtaaataa tacattccgt caaatcgcaa gctcgattgg
    21541 tacagctatt ttgatcagtg tgttgacgaa tgtgacaaaa gcaaatatcc caagcaaatc
    21601 tgtattggat aatacgccat tagcttataa agatcaggca atcaatgcca cactgtcagg
    21661 ttaccatgcc gcgtttatcg tagcgatcat ctttgggatt cttggcttta ttattgcctt
    21721 tttattgaag aaaaaagaac ctgttctaat gaaagcgggg gataatgcat gattattgga
    21781 gttttgattg tcagcttgct tgcttttgct ttaacgaacg tgttcgctaa aaaaacatgg
    21841 caaacctttt tatctttgat tttcggacta atcttcgtcg cttcactaag tttgatcgtc
    21901 gctaatttgt ccaatcactt tggaatggaa aaagtaacgg aaacaaaaac agaaaaaatt
    21961 gtttccagtg ctgattccca aggagcagat atgcttttgt ataaagcttt agggaatgga
    22021 aaagaaaaag tgtatcttta ccgaacaaat gaaaaacaag aaaaaccaaa agcaactggt
    22081 acggataacg aaacaaataa agtagaaaag acagacggcg atgctgaaaa agtgacgaaa
    22141 actacttatt gggaatacaa aaacgacatg tataaattct ggttcaatat tgctgataac
    22201 aatcacgaat atgacagccg agtgaataca ttcaaaatcc cagagacatg ggtagaatta
    22261 agtacggacc aagcagccaa gttagctgaa ttagtgaaga aacagcaaag tacgatggaa
    22321 agtgaagcaa aagcttatgt tcaagacggt atggtaaaag caatgactga aaatccaaaa
    22381 atgagcaaag cagagcaaga acaacgtacg aaagccttag ctgctgaatt ccaacaacaa
    22441 gcattcgcaa aattagtgaa agaagcaaaa ggcgaataat attttgaaca ccatgtagga
    22501 ggttgtgaaa aagctgctct gcatcaagca gtaagaacaa tcagataaaa atagcactcc
    22561 atatttttca tctgattggg cttagtgccg tagatgcaag cttttgaaca ccgtttaagg
    22621 aagaggttgt gagcctgccg tttaactctg agtcacaagg agcaatttca aaaaacagct
    22681 tctcatgttt gttgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta
    22741 ttcgaaaaaa ggaactgcga caagactttt gtcacagttc cttctttttt ttgctatgct
    22801 atactagatg taaaaaatag gagaaataaa atgagggaaa tttattcttt tgaatcaaaa
    22861 atctatgctt ccgaagtcgg caagggcggg gcatatatta tttttccgta tgatataaga
    22921 aaagaattcg gtgctggaag agttaaagtc caagcaactt ttgacaatgt gccgtatgaa
    22981 ggcagtatcg tgaatatggg cgtgaaaaat ccagatggtt cagtctgcta catccttgga
    23041 ctacgaaaag atattcgtaa aaacatcgga aaagaaatcg gtgatattgt agcagttaca
    23101 gtgaagcaaa aataaaagag gttgtgacaa aagacgtgtc acaacctctt gataaacggt
    23161 gttcaaaagc cagtttctcg gtagtaagtc aaatttatgc aaaaatgtgg aaacccatgt
    23221 ttacaaaatt tttcttacta ctcgaaacga agcggcttta tcacaacctc ttgataaacg
    23281 gcgttcaatc agctgtccct cggtattagc tcaaaaacgg caaaatatga gaagctattt
    23341 ttccattttt ttctctcata ctcaggtcat aacgctgatt tcacaacctc ttctttcata
    23401 atggtttttc accatcttta taataaactg gttctttgat atggaaatct attttttcca
    23461 accaatctaa aatcttgtaa cctaactctt ctgacatcgg aaaagaggcg ttgatttgtg
    23521 cattggagat atggtcaaca tggataccgg aagtgatcgt acaacttccc ggaacggcag
    23581 ttttgatgat cacagctatt ttttcggcta agacatcgtc tttatggaac cgaccatcgt
    23641 ggctagggaa acgtacagtt tgcatctctg tatcacgaga aaatgtagtg actgttccga
    23701 tatgtggtgt atctccgcct gttaaagtga tcaataagtc tttaccgatt actttaacag
    23761 cagcttggat cgtgaaacct agtttggtta cttcaactat cgtttctttt tccatattaa
    23821 tcaccctgcc aacgttttga atgctcattt acaatttcaa attgatccaa aagtttcatt
    23881 gtatggtgat taacaagatc ttggatcgtt tgaggctgat tataaaaagc aggaattggt
    23941 ggtatgattt gcacacctag tcgtgctaat ttagtcaagt tttccaagtg aatggcattt
    24001 aatggcgttt cacgaggaac aatgactaac tttcgttgct ccttcattgt tacatcagca
    24061 gcacgtgcaa tcaggttgtc gcctaaacca acagagatac ctgcgactgt tttcatactt
    24121 gccggtacga tgatcattcc atcggtcaaa aatgatccgc tggcgacagc agcacctaaa
    24181 tctttgtttg aataatggta atcaaacagg ctttcgaatt cagttagtga taagtcggat
    24241 tccagcttta agttctgttt tgcccatgga ctgatgattc cgtgtgtttc tatcatcgga
    24301 tattctttta gtttcttcac taggttgatg gcataaatgg tgcctgaagc gccacttaca
    24361 ccaatcacga tttttttctt ccgttgtgcc aattgttcca ttttccgcac tcctttttat
    24421 gatgtcaaaa cagttgcaga tcattcatta tttcaaatat ttttcccaat cttctacttc
    24481 ttggaatttt gcacgtacaa aatgttcttt catatcgaaa ggaactgtac cgtcaaagat
    24541 agtttttgca cttatcccac ggaaacgaat agagccagga ttatattctg gtaattctga
    24601 tgggtccaat ggatggttcc tcattcctgg gatcacgatg atatctttat ctccttggaa
    24661 acgagtgttg aaagtccaaa ctacatcgtt catgtcgaaa atatcaacat cttcatcgac
    24721 taaaatgact gtcttcaatt ctttgaaggc tgataaagca agaatagctg cttgacgctg
    24781 aataccttca tccgcttcgt tttctttacg gatttgcatg atggtcatca atttaccacc
    24841 acctgcagga ggattgtaga catttactac ttttcctgga atagctttat cgaccaaggc
    24901 aagaatactt gcttctgtag gaataccggc catagatacg tgttcttcac tgggaccaat
    24961 cgttgtttgc atgattggat tgtcttttcg gtgagtaact gctttgactt tcactacttg
    25021 tacagctgga ttagcatccc cagtatagcc aggaaattct ggcattgctt ttcctgagtt
    25081 cgtgttgata tcttcttgca ttgtttcatt tggcatgatt tctgcttcaa tgatgaattc
    25141 agcacgagca atacccactt catcgactgc tataccatca acgatttgaa taggttcgtt
    25201 acgcaaagct cctgcacacc atagttcatt gtagccaagt ggagtggtag gtggttcgaa
    25261 agtcgtgcca atggcaattg ctggatctaa accaatattg atagtgatag gcattggttt
    25321 attcaaggct tcgaattgtt tttggaagtg accaatgtgg cgtccgccag gcatgatgta
    25381 cataccgatc gtatctttat cttctagaac catacgatgg atcgttacat cgctcattgt
    25441 tttttctgga tctgatccta agactacacc cattgtgatg tatggaccag catcttcagg
    25501 agtatttgta ggtgcaggca aaatgttgcg aatatcgaag tctgcatcgg tagctagatg
    25561 aacgacttct tgtgctggag cgtctgcttt gtctactttc acaggtttga ttggattttc
    25621 tacagcgtct ttcaagaaac gaccaagtgt tcgataatca tggtgcaaaa tttcacctac
    25681 acgtttacga ctagccattg ttccgatgtt cacacgtgtg ccggggaaac ctttgatatt
    25741 gttgaaagtc atagctggtc cttcttgagt cggacgtttt actgtaccgc cagcaccgat
    25801 ataacggtaa acaccagata attctgcttc aggatcgatt tcaatatctg tttcgtgata
    25861 ttgacctgga agtgttttta gttcttcgat gacttttctt aaatcatacg gttgatctgt
    25921 catgagagat tcctccttag ttgtctttac ctatctatag taaaaggtga aaaagaggaa
    25981 aacaattcaa cttgttcggt aaagtattct acaatggaat ggatgcgttt ttttgataat
    26041 agaataaact atgatatact tatcactaaa agaaagcgca tacttagtgt tggaggaagg
    26101 aaagtgaaaa aaatggagga gtcatttcct aaagcagtaa aagtggaaaa tatagcgaat
    26161 attttgaaag ttacatttga aaacggagaa gtaaaatacg tgaaatctca ttggacagaa
    26221 gagataaccg atgccttgca atttggaaaa aaaggaagag gaaaaagaaa aaatcttctc
    26281 gcactttctc gtaatatgtg gattgggact gaagtaacga ttgaagcaga tggaacagta
    26341 ttcataaatg gaaaagacag gtatacgcca gaagagttgt ggtacaaagg aaagaaaagt
    26401 ataccagaat tataaaaaat aattaaaaaa aacagcgcga aacaaggaac atattacttc
    26461 ctcgtttctt gctgttttct actatttcag cgctcttatg actcaaatct aaatgtggtt
    26521 gtcacaacct tattctgcac gcaaagcgat agcggcgtct tttttcgctg ccattcgtgc
    26581 aggaagatga ccgccaagca tcgtcaatat agtagagacg atcactagaa taatcgcatg
    26641 gacaggattt agttgagcaa cattttttag atccgtcatg ttgtataaga tgctattgat
    26701 agggaacgta gctagccatg caataaacac acctaatatc ccagaagaaa tacctaaaat
    26761 acacgtttct gcgtcaaata cgcgagtgat gtctttttta cgtgcaccta aagcttttag
    26821 tacaccgatt tcttttgtcc gttcaatcac agatgtataa gtaatgatac tgatcatgat
    26881 catacttgtt actaaagaga tccctgcaaa agcaataagg acataagtga tagcatccat
    26941 caatccgcca gtcagttcag tcatcgttcc agctaaatca gtataaatga ttttatcttc
    27001 atctgatttg cctttattgt aatcatctag ataatccaag atcttttctt tgtcttcaaa
    27061 attgttcgga taaatcatga tacttgaagg caagctgtct ccacctaaat aactaagcaa
    27121 tgtttgtttg gcattttcgt caactttttc agtagttaag acatttacac cactgtctct
    27181 ttgagctttg acgatttctg attctttgtt ttcgttgacg atttgagtcg ttaattgatc
    27241 gctataagcg attcctggag ataagagatt cattgtagaa gaagatttca cgcgtaggat
    27301 ccccgagatc ttcaattcat cagaagcatt ctgatacatc gcatcataat ctgtattagg
    27361 gataaaattc ccagttggaa gttttgtata gtaagtattg ttgtttacta atttgaatgt
    27421 tgtaccaacg atatcatcaa aatcaagttt ttcgtcttct ttcacatcaa atccaagatt
    27481 cttcaatgcg ttgatattag tattattatt gccgtctacg atcagcacga catctgttgc
    27541 tgaggctgga taacttccgg caagtaaaga ataattgtct tttaaaaagt tttctttgct
    27601 agtgtcaagc tgtgtaggga aacttgacac tcccacacct gtcatcgctg acatcgtact
    27661 agataaagaa agagattccg tatcaggatt ttgattcgag aagctgacag gctgtacttt
    27721 gccatttaca tcacgtaaaa gattgattcc tgtagtacgg gtaaatccga tattattact
    27781 taagttagga tcgatgtctg ttacgtaatc gacgtatgtt tgatcaatct tattcgtgtg
    27841 ctgtgcttta tcttcttcgc tgacttttgc agtaatcgtt ttgctgtctg gataatccgc
    27901 tttgcttgca cccaatccgg catcgtcacg agtctggtta gtcgttacct tagaaatagt
    27961 aatcggaaat ttcgccatcg tttcagcttg cgtgttatcg atttgttttt ggaatccaga
    28021 agaaagggac agaacgatcg cgattccgat gatcccaata ctggaagcaa aggaggtcaa
    28081 gaaagtacgt cctttttttg ttcggatatt gttgaaggaa agctttaacg ctgtccaaaa
    28141 actcatcttt gttctgcgta agttaaattg atcatctttt ggtcgttcga tgtgaggatg
    28201 ggaatctgtt aagatcttac catcagaaaa ttcaatgatt cgatctgcgt attgatgggc
    28261 cagttcagga ttatgtgtga ccatgatcac taatttttct ttcgataact cttggatcaa
    28321 ttccatgatc tggatacttg tttctgtatc taacgctccg gttggttcat cgcacaaaag
    28381 gatatctggg tcattggcta gtgctcgagc gatggccact cgctgcattt gtccaccaga
    28441 taactgattt ggctttttat gcatatgatt tgtcaagccg actcggtgca atgcatcttc
    28501 tgcccgtttt cgtttttcgt ctttagaaac accacttaat gtcatcccaa gttcaacgtt
    28561 ttcaataatg cctagatgac caatcaagtt gtaactttga aaaatgaaac cgatagagtt
    28621 gtttcggtag gcatcccagt cactgtcctt aaagtcttta gtagactttc cgttgattac
    28681 catatcaccg gaatcataat tatctagtcc accaatcaca ttcagcatag tcgttttacc
    28741 agaaccacta ggacctaaaa tcgctacgaa ttctttttgt ctgaaagcga cagacacacc
    28801 atccaatgct ttggtggtgg tttcgccgac tttataatat ttttttatat cttttaattg
    28861 aagcataatc acccttctcc cgtattgaaa tattcggtat actaataagt atatcagaac
    28921 aatgatcgta gtatacgaaa taaatgttaa ctgaagatga actacaagaa aaaatctaga
    28981 aatttttcaa aaaacagagg gagatcaatg aatcgtattt tagtagtaga agatgatccg
    29041 caattacagt tgctttaccg ttctgtgttg gaacgagcgg gcttttcagt catcaccgca
    29101 ttgaatggag aggatgcgct gaaaaaacta gaagcgacac gtatagaatt aatcattaca
    29161 gatatcatga tgccgaaaat ggatggctat gacttgctag actcattacg tagcaccaga
    29221 tttgacacac ctgttctaat gattacggca aaggccgatt ttgaggataa gaaacgcggg
    29281 ttcaaattag gagcagatga ttatatgaca aaacccatcg atgtcaatga gatgatccta
    29341 cgtgtggaag cattgcttcg acgttcaaag atcaattaca gcagtagctt gataatcgga
    29401 caaacaaaat tagagctaga tgcttataca gtgttacaag atcagcattc gttgattctg
    29461 cctcaaaaag aatttctgct tctgtataaa ttactgtctt atcccaacaa gatcttcact
    29521 cgccaacagt tgatggatga tatttgggga ttagatacga atacagaaga acgaacgatc
    29581 gatgtacata tcaaacgttt aaggacccgt tttgaaaaga cggaagattt tcaaatcatc
    29641 acagtacggg gactaggcta taaggcggtt atttaaagat gaaaaaaaag ctattttcac
    29701 aattatggat ctattttgct agtattgtct tcatttcgat cttagtgaca ctgctgtgtt
    29761 ttctcggttt tattttctta ttgtctcgac attctatctc gcctgctgaa tcggctggaa
    29821 aaccaacgtt atacccgtta ttcgtctttg cggggttcag tatggtcgtg ggtacaggaa
    29881 tatcgatttt tgttgggcgg cgtatcctgc atccaatcag tgcgctaggg acaaatatga
    29941 gtctggtagc aacgggagat ttttcgattc gaatggatga acagcagaaa gtagcagaag
    30001 tacagcaact gtataaagat ttcaacgtta tggtccaaga actcaatagt atcgagacac
    30061 tgaggaatga ctttgtgtcc agtgtatccc atgaattcaa aacaccacta gcaacaatcc
    30121 aaggatatgt tcaattgtta caagccccaa atctctctga cgaagaacgc caaatatttt
    30181 tatatcggat tatcgaaagt atcacgcagc tttcacaatt aacagagaac atcttaaaat
    30241 tgaacaaact ggaaaaccag cgtatccaac tggaaaagaa agagtatcga ttagatgaac
    30301 aaatcagaga agtaatcgtt ttttttcagc ctaaatggga gaaagaacag ctagaattgg
    30361 atatcgaact tgctgcagta aattacacag gcaatgaaga gtttttgtac caagtgtggc
    30421 tgaatatcat ggataacgcc ataaagtaca atcaaatcaa tggacagatt catatcaaac
    30481 tatttgagac agccacagag attgtactgg aagtgaccga ttcaggtgta ggaatgaatg
    30541 aagagaccag agatcgaatg tttgaaaaat tttatcaagg agatacaagt cgtcaaattt
    30601 ctgggaatgg gctaggactt tcactggtta aaaaaattct agaactgcat gatggacgta
    30661 ttgattatag cagtatcgaa ggagtaggaa cgacagcaat gatacggtta agtaaacaat
    30721 aggaaaaaga ataaaacccc taaaaattct attgaaggtt tttaggggtt tttagtcgtt
    30781 tttttgtgat tagggaaatg ttcttacggt tcttttggca taaaaatcgc tgctttctta
    30841 ttgaactacg agaaatgtta gaggtgtcat tgaaacaagg acatgatgca agaaagagag
    30901 cgaaagtcat gcgtcgctct ctttttttgg gtggatagat ggacagaaaa tgtagggctt
    30961 agttttcttc ttgatttaaa tactcccaaa atgtatctga agccaaagaa tgctcaccga
    31021 ctttacgagt aaatgcaaga taactctctg ttgttggaga aatagggaga atcacagtat
    31081 tttcttttaa tagattcttg actgtttttt ccataacaat agcaatcccc atgtcatttg
    31141 caatcatatt gatgatcaag tcgattcttg ctcctttata agagattttt ggttcaaatc
    31201 cagcttcgcg acacagatct agtacaggtt gatacaagtt cgtagtgggg ccaaggagca
    31261 aaaagttctc ctttttcagt tcgcttagat ccaatttctt tttactagct aaaggatgat
    31321 ttttaggtag aactgctaca aaatcgtctg tttcagtagg caaccactca atcgaacttg
    31381 gtggggcctg gaaacttcta gcaaagtgga tcgtgtcact atgttcattg gcgtcattgt
    31441 aagttaattc gacactttcc acttctttca actggactgt aaattcagga tggcgtttca
    31501 aaaactcggt gattttggaa aaacctttat aattgatcat tgtcggtatc gttaagatat
    31561 ttaatgaaag attgttgtct ttcgtatagg tttcaagcac ttcttgcata gcatggtatt
    31621 gttctacgac attttttaca tatgggagca ataagcttcc agcttcggtc aattcgattt
    31681 ttctatgggc gcgttcgaat aatttgacgt ctaattctgt ttctaacgaa agaatctgtt
    31741 tagaaatatt tccttgggtg gtgaattgtc gatcagcagt tttggtgaaa ttcattgttt
    31801 cggctaaatc aagaaaaact tcgaatttat ggatatccat catattcctc ctacttattc
    31861 caataaagaa tactttaaca caaaaaatga attggtgtaa tcttctctgt ctgttacaat
    31921 ctttcttgca gcgaaagcaa taattacaga aatttttctg atttttttcg cttcgtagaa
    31981 aaagagagga gttttttgat gccaactgga gttttgatca atgtaggttc cgttttgttt
    32041 ggcggactaa ttggcggatt agtaggaaat aaattaagtg aacattttaa agcacagctg
    32101 acgatggttt ttggtgtgtg ttcgatgggg atgggaatct attccattgc gccgatgaaa
    32161 aatatgccgg ctgttatttt tgctttagtg ataggaacag cgattggttt gattgttcat
    32221 ttaggtaatg ggataaataa gggggctgcg ctgatgcaag tacctatttc taaaatcttc
    32281 cctagtgaaa agcttggtat gacccacgat gaatttatca gcacattagt gacggtcatt
    32341 gttctatttt gtgcaagtgg tacggggatc tatggaagtt tagattctgg gatgacagga
    32401 gacagtacga ttttgatttc taaatctgtc cttgattttt tcacagcagc tatttttgcg
    32461 tgtaatttag ggtatgtcgt ttctgtgata gcaattcccc aattcattat tttttatatt
    32521 ttgttcttat tggcaaaatt tatttatcca ttgactacac cagacatgat cttagatttt
    32581 aaagcttgcg gcggattctt gatggtcgct accggtttta gaatgatcaa cgtgaaaatg
    32641 ttcccagtag ctgacatgat acctgcaatg atcgttatca tgccactgag ctggatgtgg
    32701 acaaattgga ttatgccact gttataataa aaatcaaaga gaagccattt tttgctgact
    32761 aaaaaggatc aaatctttta gtcagttttt ttgtatttga caaactgatt ttgaaactat
    32821 attatatgtt gtataatata gattgaaata aaatattaca cttgagaata gaaggtgacg
    32881 taatgcacat ccagattcca actgttcttc ttgatggtac tgtgttagca gcattaagca
    32941 aggaagatat gtatgggtat gccttaacaa aaatcgtaca agaaagtctc tctgtcagtg
    33001 aatctacact atatccggtt ttgagaaggt tgaaaaaaga aggatatctt gagacctatg
    33061 atgaaccttt tgaaggcaga aaccgtcgat attactcgtt gacaccaatg ggaagtcgtc
    33121 atttagaaga aattattgat gaatggattg tttttcgaga ttctttagac cggttattgg
    33181 aggagagcac aaatgaatga atatttgaat gaagttgtgg atcatcttca agaaattcct
    33241 gaagacgatc gttttgattt gatccagtat tatgaggaat attttttaga ttctggaaaa
    33301 acattagatc agatcattga tgaatatggt acaccaaagc agtttgcctt aaaattaaaa
    33361 atcagttact tttcagaaga agaacaagca gaaaacgagt tagtgaaaaa tccaaaaaga
    33421 caaattcgcc tgatttggtt aatcattatc ggtttatttg cttctcctgt cctcattcca
    33481 ctagcactgg ggtttatatt ggttattggg ggactattgg ttgctttact agcgctcatt
    33541 tttagtatct atgttttatg tatcagtatc ttaggtgttg gcctattcat gatcatctca
    33601 ggaatcagtg tgtttggaca atcgattctg agtggattat tctttatcgg tgtcggtctg
    33661 ttcgccacag gaagcgcagt atttatttcg ccactgttgc tgaaaggaac aaaagggctt
    33721 ttttatgtca tgatgaactt tgttaaatgg atgggacgac gttttattac aaaaagagat
    33781 ctgcattcag cgggcgtttg aaggaggaca cataaattga gattgaaaca ttttttgatt
    33841 accggtattg gtctgatgct tactggtggc ctcctagcag gtgcaactta tacattaggc
    33901 gctcaaaaga gtctgacttg ggataatggt cctcggttga tcgatatggt tactgagaca
    33961 aaaaaaatca aggatgcaaa caaaatcgtc atccatgcag aaaatcaaac agtttcggtc
    34021 atacgcggat tggattttga gataaaaacg acttatgata agacagaaaa gccagaaata
    34081 aaggagaaaa atggcgtgtt gactgtgaat gcagtcaaaa aatcaacagg tgcaatcatt
    34141 agtttagaag acacaagtgc acaaatcact attgttgtcc ctgcagatca tgaattatct
    34201 gaattgacaa tcgaagggaa taatagtgca attgatatca acgaagtatc aatcaaaaaa
    34261 gtgaatacta agacacatga tggctgggta tcgttcaatg aatttacagg aggaacaata
    34321 actgcgaaag atgaattagg ctcacttcga ctagatgagg ccaatgtgga taacttgatc
    34381 gtcacaggtg aaaacagcaa cttccatcta gaagacagtt tgatcagcaa agaaggaaaa
    34441 gtcatcctaa acggtggtaa tttatctttg catgagatgg aaaacagtgg ctatactatc
    34501 caaacgtctg gtgaatctta tattgaaaaa gactaccagc caataacaaa tactttgtta
    34561 gaacaaggac agaaaaagct aaagatcact gctaaagaca catcgatcaa tattacgacg
    34621 gatcaagagg acaatgaaac agattgatcc tgtactcaac tggatatttt aagaaaaaac
    34681 agcaaagatc cttccaatct ttgctgtttt tcatcgctta acgcacataa ctatagtaaa
    34741 ataagaaaga aaatatctaa aaagaaaaca gggtgaaaga gtatatgaag gtagaagaag
    34801 gtcaaagaga aaagttaaag acagaaatgg aaagattaca tacttatatc actcaactaa
    34861 gccaaacctt ctatgatcca gacaaagaaa aagtgatggt taattacccg aacaattcag
    34921 aaggtcgcca attagagcaa gtgtaccatg aagttttcaa acatttattg acggtgaaga
    34981 aagagctgga ttattattct ttaccgatta tcgatacagg aatattgaag tatgatggga
    35041 agaaggaacg atttatcttt aaatctgttc gggaagattt gcctctttca gcagggatgg
    35101 atctagaggt cttggtagaa gactatttta cagaagaaaa gcactgggtt cgaacaaaat
    35161 tggattattt accgcaggca gccggaggga cacaggcaag cggttggtac atcactgaag
    35221 acaaagagtt agaactagaa ggtgtaatgg cgcgtattcg aaaaaaaaat taaaaaagta
    35281 acattagata tttattgaag gataaaggag cgcaggaagt agcatgagag aaaaaagagc
    35341 attttatatc aatggttatg cagggattgt cggattggtc gtattagctt tagttggatt
    35401 gtttttcttc tatttaggta tgtggcaagc gaaagtttgg gcactttttt taagtatttt
    35461 tctttggttg atcacgttat tgttgttaag ttcggcaact gtagtcagtc ctaatcaagc
    35521 aaaagtaatt ttgttttttg gacaatattt aggtacgatt cgagaaaatg gctttttcct
    35581 tacgatacct ttggcacaaa aaatgactgt ttcgttgaaa gttcggaatt tcaatagttc
    35641 tgttttaaaa gtcaatgatt tggatggaaa ccccatcgaa atctcagcag tagtagtctt
    35701 taaagtcgtt gatacagcaa aggcattatt cgatgtagcg tattatcaag attttgtaga
    35761 aatccaaagt gagacagcca ttcggcatat cgctagccaa tacccgtatg atacattcaa
    35821 tgagaatgat ttgacgcttc gaggaaacac gacggcagtg tctgacgagt tgcaaaaaga
    35881 gctgcaagag cgtttagcag tagcaggtgt cgaagtaatc gaaacacgct tgaaccattt
    35941 agcctatgca acagaaatcg ctagtgccat gctccaacgt caacaagcaa aagccatttt
    36001 gtctgctcga caaaccattg ttgaaggagc tgtcacaatc actcaaatgg ccttagagca
    36061 aatcgaagat ggacaagaaa tcaatttcac cgatgatcgg aaagtccaat tgatcaataa
    36121 tttacttgtc tcaatcatta cagacaaagg aacgcaacct gttatcaata caggcgacat
    36181 gaaagaccgt aagtgacata ctttttgtaa gataggaaga aggagtttag gacggattat
    36241 tgtcttgaac tcttttttta gttgttttaa agaaaatgac aaaattgaaa gaagaatgat
    36301 agttgattcg aaggcagaat tgttcgcttt tttctataat cagtatataa agtaagagag
    36361 aaggggaaaa agtgaaaaaa cttctgatcg gtctacttgt tttcatcgtt gtggtacttg
    36421 caggggtgaa aatcgcagat tacgtagtta tgggtggaga agaatactat gtacaaatta
    36481 cgacagatgg tacaaaaaca gtttcaaaat cagactctgg acaagaaaca gtcaactata
    36541 gatatatcct gcctggctat acgaaagagg gtaaggaaaa agaactggac ttcaagggac
    36601 aattgactcg accgcttcgt aaaggagcat atttaaaaat cacctggaac aaaaataaag
    36661 gtgtcacttc gtatgaagaa gtcgaaaaaa aagacgtgcc gaaaacagca ctggaaaaac
    36721 taattaaagg gtagggaaag aaatgaacaa aatagtcgaa gtacagaatg taacaaaagt
    36781 atacggaaaa aacaatgaga aaaagacgca agcattaagc ggcatcagct tcgatgtaga
    36841 aaaaggcgaa tttattggga tcatgggcgc ttctggttct ggtaagtcga cacttttgaa
    36901 tattttatcc acattagata aaccaacaga tgggcatatc cgaatcaatc aaaaagatgt
    36961 gaccacatta aaaggaaatc aactagcaga ttttcgtgcg aatgagattg gctttatttt
    37021 ccaagatttc aatttattag aaagcttgac agcacaagaa aatatcgctg ttcctctttc
    37081 tttacaaggc gtcagaccaa aagaaatcaa gcaacgtgtc caaaaaattg cagaacgttt
    37141 atccatctct catattttgg aaagctatcc ttctgagatt tccggtggac aaaaacaacg
    37201 ggtcgccgcc gcacgtgcat tgatcacaca accaacgatt ctattagggg atgagcctac
    37261 aggtgcgctg gattccaaaa gtgcccggga tcttttagat acgatggacg aattgaatac
    37321 aaaagatcat gtatcgattc ttcttgtgac gcatgatccg ttttctgcaa gttattgcca
    37381 acgtatttta ttcatcaagg atggcggtat ccatcaagaa gtgaaacgtg gagatcaaag
    37441 ccgtgattcc ttctatcgag aaatactatc tattcttgga aacttggaac agtaggggga
    37501 gattgactat gctttggaaa ttatcactta ctgggatcaa gagccgttta agagactact
    37561 tggtgttatt ctcaggatta gttatggcat ctgctatttt ttacatgttt gaatcaatgg
    37621 caagcaacga agcttttttg aaaagtaata cgatcgttgc ggctacagtt atcattttcc
    37681 gattcggttc tgttttacta gggatcatca cattcgttta tattttatat gcaaattcct
    37741 ttttgatgac gatgcggcaa aaagactatg caatgtttat gatgctggga gcaaaaggca
    37801 gaaaaatcgc acagatgatt ttttctgaaa cattcttagt agggatcgca gctactttag
    37861 tgggttcagc tgtcggtgta ggattgacaa gtatcgtcca ccgattatta gtggatcagt
    37921 tagatctcaa tatttcgcat tttacacctt ttagtattca cgggttgttg atcactgttt
    37981 tgttttttgc tgttttgttt ttattagctg ctgttgtcaa tgctttttcg attgtcaaaa
    38041 aaccgatttt agcattgatt cgtgcggatc agacaccaac aagaatcaag cagcataaat
    38101 tcttgttttt ccttgaagta gtactgggaa ttgcttttct aggctgcggt tactatatga
    38161 tgaaacacgt ggggacctat caagtctttg gtataggggt agccttagtt acgatcgtat
    38221 taggaactta tttcattttc cattctgtga tcattttctt tttatcatta ctaaagaaat
    38281 cagaacagat ttcattgaaa aaattgaaca atttcacatt gtcacagtta aactttcgta
    38341 ttcgtgaata tacacaaatg ctctcaatgg tcgctatgct gtttgccttg gcactgggcg
    38401 ctttgacggt aggattaggt tttaaaaatg aaattgcaga cttgacagaa aacaattctg
    38461 gatatgatct agtgatgaat aatcctcggg cagaagatca gcaaaaaatc aaagaacttt
    38521 caccaacact ttctgcttct tatactcaaa aagaagatgc gggaacgatt tactataatg
    38581 caagtgaatt tgcggctcaa ccattgatca tgataaaaca tgaagatgga acaccgccga
    38641 agatcacgaa agaaaaagtt cctgctaaaa aaatgaatga actgactgta caagaggaac
    38701 ttcgaagcta tgaattgccg gaacagcaag aaaaagaaat caaggtagtc agtcaatcag
    38761 aatttgatca actgaacttg ccacaatcca ctttacagct agttcaggta aaagacttcc
    38821 aagcaaacct gaaaccaatc gaaacacttg ttgctcaaaa taaaatgaac aatccatctt
    38881 tgcaaaaagt agagtacagc aaccagaaat atgaaatgta tgaagtgtat aatggtctat
    38941 tctctggttt tgaatttatg ggattcttcc taggtttagc tttcttgacc atgcttgcta
    39001 gctgtttgat gttcaagatc ttgtcaggaa gcaagagtga cattgctcgt tatgaaatgc
    39061 ttgaaaagat aggaacacga cgcgggttgc ttaaacaatc gatccgtaga gaagtaggcg
    39121 tgttattctt agcaccagga atcttaggtg tagtccatgt cttattcgga ttacaaatgt
    39181 ttggtctatt catgcaaaat ccatacaaag atatttgggt tccgtttacg atattcttcg
    39241 tattgtattt catctactat gtactgacga catggttata tactggaatc gtattgaaaa
    39301 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaagca aaaatagctc
    39361 ctcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgtttg
    39421 ttcggaaaaa gaggttgtga caatattttt gtcacaacct ttttttcacg gtcttttatt
    39481 agaatatagt aaattttttc tcgttactca cggaataagc tgttaagaaa acggtttcca
    39541 tgtatactgg aatcagtgca aatgaaagaa aagagggtgc aaggaacatg gaaatcaatg
    39601 aacaaaattt tggcgaaaac tatcacttga ttacgatcac gaatcaaaat gggacatctt
    39661 tgtctgtcag tgatcttggg gcacgggttg ttcattttca aacaataatc gataatgaaa
    39721 aacgagagtt gatcttaggt tttgatgaag caaaagaata tctagaaaaa gacccttata
    39781 tcggtgcttc aatcggacgt atggctggac ggattacgaa tggcacattc aaattagatg
    39841 atgaaacgtt cgaagtaaaa acagacccgg acactggtca ttgtttacat ggtggtgcac
    39901 ctagctttga agccaagcag tggcattata cagtcatcaa tggtgaaaac gaagcatcgg
    39961 tcattttcta tcagactagc ccagatatgg aaaacggatt tcctggaact ttagatgtag
    40021 aagtacggta tacgctgacg aacgatgata catggcgagt gacggtccaa gggatcagtg
    40081 accgcaaaac agtattcaat cctacaaatc atgtctattt caatttaacc ggtgatccag
    40141 cacagtcaat cgacgaacat atactttggt tgaatagccg attatttgct acgctcaata
    40201 aagatacaac gcctacagga gaagtagttt cagtaagtgg cacagcattt gacttccaac
    40261 gtcctaaaaa attaggtgag gtctttgcca gtgattttcg ccaatcaatg atagtgggcg
    40321 gcatcgatca tccatttttg ttgaaaaaac aatctggttt actaaatgct gcggaattga
    40381 caagcccaga tgaaaaagta tcgatccaag taaaaacaga tgcttcagga atcgtgattt
    40441 ttacagcaaa ttttggagaa gatggaccgg aaatgcgcgg gaataaatta gtaaatcatg
    40501 gcggtatcac atttgagact caagaactgc caggagcgga gcgacttgaa agttttggag
    40561 atgtcactct ggaacctaac caagtacgcg agtttgtaac ggaatacaaa ataaatataa
    40621 agaaatagaa taaattaaaa agggagtgtg acagtgtcac atcccctttt taatttattc
    40681 tatttcttta aaggcagatc aagccagaat gatttgtcta aacttaacaa ggttagtaca
    40741 ggaacagttg cagctttaac tgtacgagta gttgtttctg gtatctgtct tgccgtaaaa
    40801 ttgttccaca aatcccagat tgaagcgaca cggaagatgg aagaagcgtt gttgacgcgt
    40861 tttatcgttg aaaattctaa tcctgtgatt ggcacgccat ctgttgtcca tttgattggc
    40921 acatcgaatc gcttagacat attgaggatt tcagcgtttg cttcttcagt tgttgctgct
    40981 tcttttttcg gttcatcata ttttgtcagg tcgtacgctt cgatgatcgc gttcaatttt
    41041 tgtgaatatt ggcgatctgt agcataacgt ccttccaaat atttggttgc ttctttgtag
    41101 tcttttgttt ccgacttcca agttggtttg tagaaatcag gattgccagt cagtccattt
    41161 ttgatcaagt cggcatagtc ttccaaactt tccttgtaag aaggatattt acggaattgt
    41221 gcgcggattt ggtagctttg tccggaacta tcttgttcaa gtgtattgaa ggtcacgctg
    41281 cttcctttat atgatccttt gataccaaaa aggttatgat ttggttcgct tgataataag
    41341 ctgtttccag atccactttc aaggatagct tgagccatca tgactgatgc gtagattcct
    41401 tctttttgcc cgatttcctg tgcatcatct gcaatctttt tcaagaactg atcagttgtt
    41461 tggttttgac tgaaatggaa cgagccatct aatccatcta aaatagatga gccgcctgtc
    41521 agcgaacctg aagcagcagg taaacctgct aagtttactc ggacagcaaa atcaggcgtg
    41581 aaatctgaaa tagaagttac tcgaacttta tctcctggtt gaggcgcatg gataaattgt
    41641 ccgttcccaa tatagatacc tacatggtga gtaacaccgg aagaaggtcc ccagaagacg
    41701 aggtcgccag gttgggcctg acttactgga atctgcgtac ctgcatattg ctgttcgcct
    41761 gtccagccac cgatataatg accggttgct ttcatatata cgtaataagt caaaccagaa
    41821 cagtcaaaag tatttggacc ttttgctccc catacatatg gttttccaag atgcttgaat
    41881 gcttctcgaa cgattgcttg ttggacattc gttgtatttg taggcaatac agcatcaatt
    41941 ggtgatttta caacaaaatc agaagaagca gaagatggat tctctatacc gctttgattt
    42001 gctgtatttg gacgagatga agcattgttc gttccgccat tcgatgaacc attgtttgga
    42061 tggtttggtt ctctctgagt atttccattg ttattcgatc cagatgggtt tgtttgattc
    42121 gaagaattcc cgctgttcga attattgtta ttattgtttg aattatttga accattatta
    42181 ttgttgttat tacccacatc tggtttgttt ggcgtttccg gatctggtgt actagggact
    42241 tcttcactag gatcagttgg tttctctgtt gaagtatccg gtccagcttt aaattctgca
    42301 gtaatagata aattgttcgt acctactgta aaggtctttg catcataatt tacttctttg
    42361 gcaccattaa cagcgactga agcaatttgt tcaccttcag ctggcgtaat agtgtaagtt
    42421 accttcgttt ccgctaataa accttctacc gttccacttt ccgtaatatc caaaggttgc
    42481 tgttcagggg aatccgctgt cgaagcagtc agttggatct taccaatcaa agaaggagca
    42541 attgtaaggg aatatgtctc ggctggcttt tcattttctg atggatcagc cggatcaatt
    42601 tcagttggct cggtcgatcc tgttgttgag ttagttgttc cagttgggtc ttctgtaaaa
    42661 gaatccgttg tttcactagt agctgagctg gatgtagtcg tatctgttgt gactgtttga
    42721 tcagctattt gattatctac ggcaaatacg gtattggtcg acaaaacaga cggagcaatc
    42781 agccaagtag cagctagcaa acgtatcgtt tttttatttt tcataataaa ctagtccttt
    42841 tcctttcatt gattttctgt cttattcagc attcattaga cattttttgg aacagataat
    42901 cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt attctaaata aaacaatttg
    42961 taaataagca acgagcattc attcgttttt ctttgacgtt cattttattg catagaccac
    43021 ctttgtttgg ggataaacaa gtggggataa aaatggttat gcatatgtaa attataccaa
    43081 agtttggaaa gtatcacaaa aatttataaa aataatgatc ctaaaaagaa aaagactgaa
    43141 atatgtgata aacatctcat acttcagtct ttttcattaa ctattcataa gacattaggt
    43201 cttcatcaaa cccgctaacg agagtagtga tcattgctac gactagacta acagccagtc
    43261 ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg caatgtggtc agactaccaa
    43321 atacaaaagc gatcgctttc acttgggcaa atcccattac agctccacca gcagctgcac
    43381 caatgcaagc agcaacaaat ggttttttca atgggatcgc aattccgtaa acagttggtt
    43441 cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc ggagatcgaa cgggtttttg
    43501 aacgtttact tcgcagaaat acacctaaag ctgcaccagc cattccaaaa ttggctgctc
    43561 ctgtcaacgg aacgattcgg tcgaacccat aagcagagat attactgatc atgacagggt
    43621 ttacagccca ttggatacca aatattacca aaatattcca accaccagcg atgattgcgc
    43681 cactgatcgg tccgttaata tccatcatcc aagtaattgc ggcagctaaa ccttcgccaa
    43741 ggtaaacacc cgcaggcccg aataccatta acgtcaaagg aaccgtgata actaatgaaa
    43801 gtaaaggtac aaatactaat tgcatatttt cgcgaatata ttttttgagg aatttttcaa
    43861 caaaagaata aaagaaaata cctaaaatcg ccggcatgac agtagacgta tactgcatca
    43921 gtactacagg aatccccaga aaatcagtag ctgctttgcc actgtctgaa agtaaagagg
    43981 tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc aatatatcca ttcactttaa
    44041 acgttttggc agcagaaaaa gcaagaaaga ttggcagaaa ataaaagacg gcattcgacg
    44101 cagcagacaa aatatgataa gttccgcttg tttcactaag ccagccaatc gtagtcgcta
    44161 aaacaaccag accttttaat ataccagaac cggcaaacag cggaatgatc ggcgtaaaga
    44221 tttccgatat caaaccaaat agtttttcga tcggatttag cttttctttc ggagctgaat
    44281 gatcgatttg acgcgtcaat tccttgtaag caggagccaa tccctgatcc atgattattt
    44341 ctgcatatct atcgagtaaa atgaaatact taatggaagg gatttgctgt tttaattttt
    44401 ctgcattggc tactggctgt tttttcaatt tgatacgaag ccgagtcgca catggaagga
    44461 cagaatcaat attgtctttt cctcccaact gttcaatcag catatgaatc tgctcggaca
    44521 gtaccatctg ttcaggattc attgtcgttt ccattagaag accactccac tcactaatat
    44581 aatattggcg taaggtgtca tttcgcctgt acgaataaat gcatgacagt tattcaactc
    44641 ttttttcatt tcttcatgtg ggataaagct aatcggtgta tctggtaaca ataatttgat
    44701 tttttccaat atttccgggt tcattgtctt tatttcttcc gccaattcaa tcgattgtac
    44761 ctcaagttct tctaaaacgt tttctaaaac agataaaaaa cttggaagac cgtttgagac
    44821 agccaaatct attttttcag taccaaaggg gacaggcatg cctgcatccc cgatacttaa
    44881 tttatcaaag tgccccattt gtgcgattac tcgtgaaata tctgaattga ttacttttcc
    44941 ttttttcatg tttgtttctt cctctcattg ttaattttag actgctgatt gttctaaagc
    45001 ttcgcggtaa gggatagaag gttgcgcacc ataagactgc acagcaacag aagaggctag
    45061 atttccataa aggatcgctt ctttcaagtt actaaggtct ggctgaagtg tgctgcttaa
    45121 tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg gcatccactt taaaagcagg
    45181 gacgataccg tgttcttttt ctgtatgata gaaagcgccg gcactgccta gtgtaatgat
    45241 tactgttcca atacctaatt catggagttt ttcggctgct gcgaccaaac tgttatgatc
    45301 tgtcactctg attcctgtaa tgatttctgt ttctgtttca tttggtataa tgatatctgt
    45361 cttttcaagt agttcagtag gaatcgtttc acgagctggt gcaggattca ggatcgttgt
    45421 ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc gcatctaagc tactttcaaa
    45481 ctgagcaata ataaagtcac tgtttgtaat cagttgcttg ttttttagga cttcttgttc
    45541 atggaaagca ttatttgccc ctgcatggat caagatacta ttttctcctg ctgcgtctac
    45601 cataatcatt gcttgtccgg ttgtggcgcc ttggatttca gctatcccag atgtatcgat
    45661 gttttctttt gtcagtagat ctaataactg ttgtccttcg ctgtcagccc caacgcctcc
    45721 aataaaagat gtccgtgcgc ctgaacgttt ggctgccact gcttggttgg cacctttacc
    45781 tccgccagcg tgaaaaatct catgcgcatg catcgtttcg cctggcttgg gcatgttggt
    45841 taaacgaagg gtagtgtcca tattaatact tccaattact gtaaccgtgt tcattttaca
    45901 aacctccaac tatctcattt attttgatgg gaaaattatt ttttaaaacg ttttagtaaa
    45961 acgttttaat agttcgataa aaatatagca caccttgaaa gctatttcaa gggtgcagtt
    46021 gaaaatcttt tttctaatcg tacaggtaat cttttttctt cccattcttt ttctgggaat
    46081 tggatccgat ctaacaataa cttggcagat gtttgtccca attcgaagat tggttgtgcg
    46141 atcgttgtca gtttcggctt gatatactcg cacatatcta tattatcata gccaataatg
    46201 ctgtagtctt cagggatgct ttttccagct tcctctaatc cgcgatataa tccaaaagct
    46261 aattcatcat ttaaagcaaa cacaccagtt gcagcagaat caagaagttc tgctgtgatc
    46321 tggtagccac cttgttttga gaactgtgta ggtaagagga tcagttggtc gtgagggatt
    46381 tggtaaacat caagagcaga tttaaacccc tcaatccgag cgtggacatt ttccggtgga
    46441 ttttcaggat agacaagggc gatcgtttga tgtcctagac taagcaagtg catgcccgct
    46501 aggtaaccac cgcgaaaatc gtctgttcgt acggcatcac taaatccttc agattttttc
    46561 tgatccaaaa caatgaaggg ccggccttgt tttttcaaat tttcgttgat tgcatctgta
    46621 gagacagcag aagtcgcgat aatgaatcca tccacacctc gacgtgtgag ctcggctaga
    46681 tattcgattt ctttttgatg atcagaatca gcattacata atatcgtgac gaaattctgc
    46741 ttatacaaga tatcctcaat tcctcgcatc agtgtactga agaatggatt cgtgatatca
    46801 ggaacgagta caccaattgt tttcgtttcc ttggtaatca tttgtctagc aaaatagtca
    46861 ggctgatagc ctaattcttc tttggcagct aaaactttct gaacagtttt cggactgaat
    46921 ttctccgtat ttccatttaa tatcaaggag acagttgtga ttgaaacgcc ggaaagttta
    46981 gcgacttctt tgattgtgat cttttttttc accatgttca tactcctatc taaatcctgt
    47041 ggttattttc ccatgaaatc aaaaagaagt aaatgaaaat cacgtgtgcc agtacttatt
    47101 tgctttgttg gatagcttcg attatttgtt tcgttgagcg tactcgtgca gttttcggaa
    47161 atatcttttt tatgaacaat tgatgggatt ctttgtcacg atcactgcaa gcatcttcag
    47221 caagcacgat atggtacccg tgttgaaagg catctaatgc agtagcatat acaccgtttg
    47281 ttgtcgctac accgcttaaa atgatagttt cgattccacg ccggcgaagt tgaagatcaa
    47341 gagaagtacc aaaaaaagca cttggattgt attttgtgat agtcaacata ttatctgtat
    47401 ctttcaaaga atccttcaaa agtaaatcag tgaattctgg aggtacagaa gcagtcattc
    47461 cacccatatc agtttgttga ctcaaataag ggaaagtgga gatgtctacg ttcactaaaa
    47521 ctttcaaggc agcggtacgt ttcaatgttc ttactaacaa atcattgttt gtcagaatag
    47581 atgctgcagt gtgtggagag agtgtaccgt tatttacgat ctctttttgc atgtcgatcg
    47641 cgacaaaagc agtttttgat aaatcaaaaa tcatattttc ctttaaatca ttcatgatgt
    47701 tccttctttc aaataacaat gttttttatg taagtaaatc ataaatcgtt tatttgtcaa
    47761 ctaaattaaa ggattccttt ctttagtcta gagaaattca gtggtaaaat aaataagtgt
    47821 gagaaaacgc attcgatgca tagatatagc gagaaatatc gtgaaaatgg aggaatcaga
    47881 gtgaagtatg tcgaagtaaa aaatgaatac aaacgataca aaatgggaga aacgaccatt
    47941 acagcaaatg acgatatttc tttttcaatt gaaaaaggcg aattagtgat tatacttggt
    48001 ccaagtggag caggaaagtc aacgattctt aatattttag gtgggatgga tacacctgat
    48061 gaaggtcaga tcattattga tgacacagat attgcgcaat tttcagataa acagcttact
    48121 gcttaccgac gaacagatgt agggttcgtt ttccaattct acaatctcgt tcctaatcta
    48181 acagcaaaag aaaatgtgga attggcaacg gaagtttctc ctgatgcgct tgatcctgta
    48241 gaggtattga ggcaagtggg cctagctcat cgattgaata actttccgtc ccaattatca
    48301 ggaggcgaac agcagagggt ttcaattgct cgtgcactag ctaaaaatcc caaacttctt
    48361 ttatgtgatg aaccgacagg cgcgctggat tttgaaacgg gcaagcaagt attgaaatta
    48421 ttgcaaaacg ctagcagaca acacggcaat accgttttga tcattacaca taattctgct
    48481 ttagcgccca tcgctgatag agtcattcac attaatgatg ctaaagtaag gtctgttgaa
    48541 ttaaatgatc atccttcatc cattgatgag attgtatggt agggggattt tatgaaaaca
    48601 agaacatatc ttaaagcagg ttttcgcgaa attcggcaat ctaaaggacg attcattgca
    48661 atcatcctta ttgtcatgct aggaacactt ttatttgtag gtgtcaaaac tgctggacct
    48721 gtcatgcaaa aaacgatgga tcactatgtt ggtacagcag gactttctga tttgcaaatc
    48781 gtttctactg gtggcttgac acaaaaagat attgctcaag cagaaaagat cccagatgca
    48841 aaagtagaaa caggaaaaca gatctattat tccaatactg gcaaaaacga agtgattcaa
    48901 atattttctt ataacaaaaa taacaagcag aacaagctac aattaactga tggaaaactc
    48961 ccggaaaaac caaaccagct tgtattggat gaaaaagcgg aagatgaagg gtataaaatc
    49021 ggtgatattt atcagattga ttcagatgaa ttgaaagaaa aagagtatac catcacgggc
    49081 tttgtccgtt ctccattgtt catcaacaat ttggagagag gatacgcgaa cgtcggaaat
    49141 ggtagcgtcg attattttgt ctatttacct gaatccgttt ttaaaacaga tatttattct
    49201 gtcttatatc ttgattttac aaacgtaaag aatttgaata cgtacagcaa agcttacaaa
    49261 gataaaatgg agcaaaatca agaaaaggca gaaaagtatt tacaagatcg acccgaagaa
    49321 cgcttagaag agctgaaaaa atcggccaac gaagaattag aaccggcaca gcaaaaaata
    49381 gcggatggca aagcacaaac aacgcaagca agaacacggt tagaagatgc taaaaaacag
    49441 ttagcgcaac agtcagcaac tatccagcag cttcctgaag cgcaacgaac aatcgctgtt
    49501 caaacattat ctcaacaaga agctcaactt gctgaacaag aaaggcaatt agttgagaaa
    49561 gaacgtgaac tggcctctgc agaggaagaa ttgaatcgaa atgcagaaaa aattgaaaat
    49621 ttaactaaac catcatacct atacaatgaa cgttcagaaa atgtcggttt tcaagaattt
    49681 ggtgatctgt ctgatcgtat cgctgcgatt gccaatgttt ttcctgtttt ctttttcttt
    49741 attgccgcat tgatcacctt tacgacaatg acacgtatgg tagaagaaaa ccgcagggaa
    49801 atcgggacat taaaggcgtt aggttatacg aaagttgaaa tcgcaaaaaa atatgcaatc
    49861 tatgcaagtc tagcctcagg gataggtatt gttttaggaa cgatattagg aacgaatctt
    49921 ttgccaagga tcatttttga gctgtctaac gagcggtatg atattgggag tgcgcttatt
    49981 ttttatgact ggccgccgat tttacaagca gctgccgcat tcttcattgc cgcatttggc
    50041 gctgcaatga tcgttttatt caaagattta agagaacggc ctgctgcatt gcttcaaccg
    50101 aaagcaccga agccagggaa acgcattttt cttgaataca tcactccgct gtggtcacgc
    50161 cttagcttta atcaaaaaat cagttaccga aatctcttcc gatataagtc aagaatgctt
    50221 atggcgatta tcggaattgc tggatgtgcc gggctgatgg ttgcaggcgt gggattgaaa
    50281 gattctctaa gttctgtttc agccaaacaa tttggaccga tcattgacta tcaagcaatc
    50341 gttacattgg attcatccac ggaagatagt cgtcaaaaag cagcggatat gctgcagaag
    50401 caagaaaaag taaatgagat cctagctatt catactcaaa ccgtagaatt gagaaaaaaa
    50461 ggacatgctt cgcaaagtct gacagtgatc gtaccaagta aaacagctga tttggatcgt
    50521 tttatccatt taactgatct tgaagagaac ccacgctcct tgcctaaaac aggaatcgct
    50581 attacgcaaa aagtagcaga actatttgac ttatctacag gagatcatat ttcgctttat
    50641 gatgaagatc aaaaagaatg gaaagtcgaa gtaaaagaaa tcgtgaaaaa ctatctaggt
    50701 agctttattt atatgtcccc tgaatattat gcttcagtta ctgatcaatc gatgatggat
    50761 aatgcatttt tgctgcgtac agataagatg acaacaaaag aagaagaaat gctttccgag
    50821 aaactgcttg ccacagacgc tgtgacaaat acgtctttca tgtccaaaca aatcgaaacg
    50881 caagaagaat caatggcgaa cttagactcg gttgtcttaa tctttgtcgt attatcagga
    50941 ctgctggctt ttatcgtctt gtataatcta actaatatca atatttccga gcgtgtacgc
    51001 gaattatcta caataaaagt ccttggattt tttgataaag aagtcacgat gtacattgtt
    51061 cgagaaaata tcatctttac tcttttagga atcatcggag gatttggtgt aggatatata
    51121 ttgactgact tcatcttgca acaagcgtct atggagaatg tcatctttcc attagtgatc
    51181 acatggtggg cttatgtctt atcagcagga ttaactatcg tatttacggt tattgtgatg
    51241 attgttaccc atttcaaatt aaagcatatc gatatgattg atgcgttgaa atcgaatgaa
    51301 taaaaacaga ggttgtgaaa tcagcggttt gacctgagta ttagagaaac aaacggcaaa
    51361 atagtttttc atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc
    51421 atgaatcaga ggttgtaaaa aagccgtcct gcatcgagca aaaagaatac tcaaataaaa
    51481 ttagctcctc atgttttaca tttgagaaga ctgattgccg tagataaaag cttttgaaca
    51541 ccatttatag aagaaacaag aatattttgc tattttaaac aagaggatag acaaatacat
    51601 gtctgatctc ttgtttttta ttgggcaaca gacctcttag aatgtataaa gataaatttt
    51661 acaaacgctt tcggttcagt tataataata aatgataact tgtatatata agttgtggta
    51721 ggtaataaat cagttgaatg gaggtaaata tccatgacat ttaaagacaa agtcatttac
    51781 caaatctatc ccaaatctta caaagacagt aatggtgatg gaattggaga tttaaaagga
    51841 atcaaagaaa aactcccgta tctgaatgat ctaggcatcg acatgatttg gctaaaccct
    51901 atttatccaa gccctcaaaa agacaacggt tatgatatct ctgattacac agccatcgat
    51961 cctatctttg gtacgatgga ggaattcgaa cagctaatag ccgaagcaaa gaaataccgg
    52021 atagaaatca tgctcgatat ggtgctgaat cacgtttcga cagaacatga atggttcaaa
    52081 aaagcactag caggggacca gcattatcag gacttcttca ttttaagaga agagccaacg
    52141 gattgggtat caaaatttgg tggaagtgct tggtcgccat ttggagatac ggggaaatat
    52201 tatttgcact tatatgatcg gacacaagct gacttgaact ggcggaacca agcagtaaga
    52261 gaggaactat tcaaagttgt tcgtttttgg atggaaaaag gggtaaaagg attccgtttc
    52321 gatgttatca atgttatcgg taaagacgaa caattgaaaa ataataaaga aaatgatggc
    52381 aaacctgaat atacggacaa accaatcacc catacgtatc taaaagaatt gaaccaagca
    52441 acgtttggga cggattccac tatcatcaca gtcggtgaaa tgagctctac gacgatcgaa
    52501 aattgtatct tgtatacaga acctagtaga gaagaattat caatggtctt taatttccat
    52561 catttgaaag tcgattatga aaatgggaat aaatggtcta ctcctccatt tgattttgca
    52621 gaattaaagc gactatttca tacatgggga gaaaagatga gtgagggtaa cggctggaat
    52681 gctctatttt ttaataatca tgatcagcca cgtgcgctca atcgatttgt agctatcgat
    52741 cagtatcgaa aacaaggagc agagatgcta gcaaccatga tccatttgaa ccgtgggacg
    52801 ccctatattt atatgggaga agaaatcggc atgatcgacc cagactattc atcgatttcc
    52861 gactatattg acgtagaaag tttgaatgct tataagttac tccttgaaca aggattcaca
    52921 gaaaaagaag cgtttcgtcg gatcaaagca aaatctcgcg ataactctcg aacacccatg
    52981 caatggtcag acacagaaca agcaggattt actacaggga aaccttggtt aaaagttgga
    53041 ggcaagctag aagagattaa tgtagaaaaa gaactatctt ctgaagattc tattttgtcc
    53101 tattacaaga aattgattcg gctgagaaaa acatatccaa tcgttgcaca aggagattat
    53161 catgcttatg aagcaaatca tccggaagtt tatggctttc ttcgccaatt tgaaggacag
    53221 cagctgcttg tattaacaaa tttctatgat aaagatacaa ggatcgccat cccagaagga
    53281 ttcacagaag ccaatattct tatcgataat tattccgata ctgttcttca tcaaagtctg
    53341 gagctaaaac cataccaggc aattgctttg ctgaaaaatt aaaacaaaaa cacaaaacgt
    53401 cgttcctacg agctattctc gtaagatcga cgttttgtgt tttatttaaa ataatcttct
    53461 tcagaaaatg aagagttttc atactctcct ttatcgccat aaaatttatt gtagcgttgt
    53521 ttgaatttac ggaaaataaa agtaacgagt aatttgacta acgtatagat aggaatacca
    53581 aaaataacgc ccacaattcc cattagatca cccatgacaa gtaaaacgat taagatcgta
    53641 atcggatgga tctgtaaacg atcacccatt actcgaggaa tcaccaagtc accgtggaga
    53701 agttgtacgg caaaccagac aatgacaaat ttgacagcca taaaagtaga gtcttggaat
    53761 gcaatgatca aacctggaaa gaaggcgata aacggcccaa tatatggaat gatacataac
    53821 aacccagcag aaatagctaa aacactagca aattctaatc cgatcagtaa gaaacaaacc
    53881 cagtagactg ctccaaggat aaatgaagca atgatttgcc cctttagaaa tgaacccaat
    53941 tgttgattag caatctttgt caaattttta aaatctgtac gaaatgctgg tgggacaatc
    54001 gctaaaactg actgataaaa tttttgagga tctctcaaaa ggaaaaaagc gacaattgga
    54061 ccagtaaata aagtaataaa aatagctgta acagttgaaa aaatgttgct gatccgttgt
    54121 gcgccacttt cccaataatt tccaataaat ccagctatat cagacgtcac gtcgtcaata
    54181 gaagcgaaaa attgattaaa tgaatcggca aaaggtgttt tctccaaaaa ttgtcggaga
    54241 ctctcttgaa aatcttgaaa caaatctggc aaactactga tcaatgtctt tgcctgttct
    54301 tcaagaaaag gaataagcca tatgcttcca agccccaata aaaataaaat aatcacataa
    54361 acgataatga ctgacaagtt cctcgaaagg tgcttgctca aacgtttgat gactggttcc
    54421 aaaagataat acaagatcag tgcaaacaat aatgggggca agatagtagc gataatgatt
    54481 ccgacaggct tgaatataaa cgagatctga tggaaaaaat aaatgatcag cgcaagtaag
    54541 ataactattc ctaaaacata gtaagaggtt tttcctccta aaaagcggat caaacgattc
    54601 gttgtatctg tttctttttt cactcttttt cctcctttat ctataaattc attatagaca
    54661 aaagcgggta taaaaggaaa atgatagagg ttgattgcaa aaatatcgtc aatcattcat
    54721 ataaactttc tcatcaatta aaataagaaa acgcttgaca gttcttttaa ccatatgata
    54781 aattaaatcc tgttgctaaa acgtaactat tacggtttgc gaaaagcgaa tcggaacatt
    54841 caaatgaaag aagatgagaa gaggagtgtc ataaatgaaa cgattgatag gtatattggc
    54901 catgctggtt ttagcaggta tgttaactgc ttgcggggca agtgggaaag ccgaggacag
    54961 taaagaaaaa ttgagcgtga tgacgacctt ttatcccatg tatgatttta caaaagcaat
    55021 cgtcggggat gaaggagaag tagagttatt gattccagca gggactgatt cccacgatta
    55081 tgaaccttct gctaaagaca tggcaaagat ccaagataca gatatctttg tttataatga
    55141 tgaaaacatg gaaacatggg tccccgcaat ccaaaaaaca ttacaagaag gcaatgtcca
    55201 tacgataaaa gcgacagaag gtatgctttt acttccggga agtgaagaag gacatgatca
    55261 tgaccatgaa cacggggaag aaggccatac acatgagcta gacccacatg tatggctagc
    55321 tccaagttta gcaatcaagc aggtagcaaa catacgtgat cagctgattg aagcttaccc
    55381 agaaaaacag gaagtatgga caaaaaatgc agcagcctat accgagaaac ttcaagcact
    55441 ccatcagctt tatcaagaaa cctttaagca agcaaaacaa agatcttttg tcacgcaaca
    55501 tgctgcattc aactatctag cgttagaata tggattgaat caagtatcga ttgctggtct
    55561 ttcttctagt gaagaaccgt ctgcggcgag aattgctgaa ctgaaacact ttgttaaaga
    55621 acatggaatc aactatattt attttgaaga aaacgccaaa gatagtattg ccagaacgtt
    55681 agcaaacgaa gcaggtgtat ctttagaagt attgaatcct ttggaaggat tgacgaatga
    55741 acaaatagaa aatggagaaa actatctttc tatcatggaa gcaaacctcg aagcgttgaa
    55801 aaaaacgaca gagacagaaa acccgctaga agatactttg acccccaaaa aagaaaagag
    55861 cgtctacaac ggttattttg aagacgagga tgtacaagat cgctctcttt cagattgggc
    55921 aggaacttgg caaacagtag atacgtatgt agaagatggg acttttgatc ctgtttttga
    55981 atataaagaa aaattgaatc aggataagac agcacaagaa tataaagaat attatacgaa
    56041 gggctatcaa acagatattg cttcaatcaa gatcagtgag aaagagatgg catttacgtt
    56101 taagaatgga acaacgaaac gttcaaatta tcgttatgta gggaaagaaa tcttgaccta
    56161 cgaggcagga aaccgtggcg taaggtatct attcgaagca gaagataagg aaagtggcgc
    56221 ttttagatat attcaattca gtgatcacac gattgcaccg gctgactccg aacattttca
    56281 tttatatgca ggaaatgaaa gtcaagaagt attgctgaaa gaaatggaga attggccaac
    56341 attttatcca gatacaatgg atggacgtga aattgcacag gaaatgcttt tgcactgata
    56401 ggcaaaaaag aaccgaaggg tttggatcgg ctggtcattt agtcggtcca aactattcgg
    56461 ttctttttat gaaatcaatt tctttaatcc acggatagag gctgggatgg tctcgccgtt
    56521 tgccaacgtg acttctctgg tttttttatt aatcgaccgc acattttgtg tattgataac
    56581 ataggattta tgacaacgga taaaattccg atgcattttc tcgatttctt tgatcttctc
    56641 atagaattgt aattgggcat gagtcaaatg gacttccagt ttatgtggcg ctcctagcga
    56701 agtagaaaaa tattggatgt ctttgatcgg taaaaatttg atctcatcat taaactctat
    56761 tttgaagata tctttttgga tttgggacat ttgaaaatct tcgaaaattt tgctcaggat
    56821 atgctcgaca ttttctttta aatcataaag atcttctttg acgatataat cagtcggttc
    56881 gatgttgctt tttaagattt tcatcgacat ttccgtatgg gaagtcacat agattatttt
    56941 tcctaaagga tctaattcac ggatcaattt accgatcgct acaccatcca tttcactgtc
    57001 ttctagttcg atatctaaaa agtaaatacc aaaagaagaa gttctgatct tggctgcatt
    57061 gagaagttct tccggatcag aggtagaaat atggacataa ctgtctaagt tttcaatcat
    57121 gattctattt ttgatcaccg ttgataaaac ttctaattgt tttggatcgt cttcacaaat
    57181 ataaatggga atcatatcat tactccttta ttttatttgt atataaatcg tttgagaaaa
    57241 acgctggttt tcaattgatg tttcaaggaa aagataagga ttggtctttg tcaattcatc
    57301 caagataaac aagccaaggc cttcgttttt tggatttgtt tttgttgaga accctttttg
    57361 tttcaactgg tttaacggta atgtatttgt tgttgtattg atcacacgaa tcatcacgga
    57421 atgattcttc ttgagtatgg tcaattcgat ttttttcttc tttgcagcac tagctccttc
    57481 aatcgcattg tccaagataa taccaagcat acgtacaagg tttaccgtat gtttcgtatc
    57541 cagttcgatt ggctcaggaa aatgaagctg gacgatcaac cctttttcct gtgccatcat
    57601 gagttttaat gacagtaaac tacggatctc aggaactttc atattatcca acatacttag
    57661 attggccgga aaaaggttca tcatctggcg tgtaggtaat atcgtttgat cataataaga
    57721 ccgtaatcct tctaagtcgc cttctgaaat atagctgtga agagaaaaaa gcagattttg
    57781 gtaatcatgt ttgaattctc tcaagtcttg atagtttttt tctagatctt tgatataatc
    57841 ctgttgcatc ctgacttgtt gctctaataa ctgatcagct aatttcttat tagagatttt
    57901 ccaataataa accaagatga tcaggccaac acacagatat agaatgaaaa aactcgtatt
    57961 caaagctaag acatcaggtg tttcaccaga aaaacgggta taaaaaataa tgatatagta
    58021 tgtaaataat atcaatgtcc cgatcgtacc gatcatacgg cggtccacag tgtgaaacca
    58081 agtctctaac gattttttta aacaaaaagc taataaagca gataaaaaag cggataagac
    58141 aatgtgaaaa tactgcaatg aatctcctgg ttcaataacc gaagatttca aaacaaaaaa
    58201 atcgccgatg cttgttaagt gatctcctaa aagcgacgtc aataacccaa aaggaagcgc
    58261 gtaaaccaat tgttgacgcg aagtcgaaaa gaagaaaaaa gtaagcatca gcattcctat
    58321 agcaattaga gcagtaaaaa tccctaaaat cgaatagacc catccaatca aaatacaaga
    58381 aatcagaatg actcccgcaa acagatattt gtatcggatg tgatggtcaa tcaataagat
    58441 acaaattgtg taaatcaaat attgcaacgc tatggaccat aaaaacataa agtacagctc
    58501 ccattgattt ttaccaaatt atagcacaat tggcgtatta tttttttaat ttttcaggga
    58561 ttttgaattc agaactcata ccaaagcagc agccagatac aaaaaagtca gataagttag
    58621 ttaattgttt aatgattgtt tttttcatga aagattcctc caaaattcat caagtagttt
    58681 gtaataggaa gcaacgtagc tatttctaaa acaatcccaa gtagcaaata ggtagaaaag
    58741 taagaagctg agaaaaataa cgtaatgaga gaaaaaatta gaaacatgct gatcgccttc
    58801 tttaataatg ggggacgttt ctcgggttca atgggattga ttgctgtacc tcttgatcca
    58861 tagcgaagca gtaacaaaaa gcagctgatt aacaaaaaga ccatcattgg gaaggagata
    58921 gtcaattgca gatgtttcat caccaaggga acgccccata aataaagtag tgtccacagc
    58981 agacaaacga attcagagtc ggaatgcaat ccataagcat atctgcggat gcagatataa
    59041 gaaagaaagg tgattcctgt ttccgcccat gagcctgtca ccagagaaaa gactagaacg
    59101 accgctagtt ttccaagatt attcaataaa acttctaaga caaattttac ttggacatac
    59161 accatatctt cttccgattt gtctccgctg aataaggctt ctaataattt gttcacaact
    59221 ttatctttca tctcatacgt acctccacca caaattttat gctaaaaaaa ataaaaaacg
    59281 tcttttgttt cataaaaagt ggttttgaca tcaaaaccgt catgatctcc taaaaaaatc
    59341 ttcgttttcc aagaacctta ccaaaaaaag tgacagtttt gatacgaaaa gtacttttta
    59401 tgacaaattt gtgctttttc gagtgattta tgttaaaaaa ttactgacct tataaaaggt
    59461 gatggaggga ttcttatgga aaatgaacaa ttgtttgaag tcggcgaaac atatgcattt
    59521 aaatctgatt tatttgaatc accaataaaa ggaaaaatag aaaaaatcta tgaaaatagt
    59581 gtgatgatac gtgtcatgaa atgcgcagta gaagacaatg ataccgctca taatctgcac
    59641 tggcagttag ttgtacgcaa atcaagtaca atagaagaat aaaaacagat aaaatgttga
    59701 gagcataaag aggttgtgag tctcccgttt agctctgagt tacaaggaat aatgttaaaa
    59761 aacagctttt catgttttat gaatattgcc ttgtgaccga ggtgttggca ggagaatact
    59821 gtttatttga aaaaagggct gcgacaagac ttttgtcaca gcctttattt tcatgaatag
    59881 gagttacttt tggctacttt tagagaagta gcttttaagg agcagataaa gtcaaggctt
    59941 ttatttctat aaatgaagta gtaaaatgaa gaacagattt tattacagga aggatgccga
    60001 taattgtgat cattgttgca ggaacgatcg gtgcagggaa aagcacacta actgaaatgc
    60061 ttgcccaaga tttagaaaca aaaccttttt atgagaacgt ggaagataat gaagtattgc
    60121 cgctatttta ttctaatcca gaaaagtata cttttttatt acagattttc tttttgaaca
    60181 aacgcttttt ggcaatcaaa gatgctttca gccatgatga caacgtacta gaccgctcca
    60241 tctacgaaga tagtatgctg tttcatttga atgcagattt agggcgtgtt tctgaagtag
    60301 aagtaaagca gtatgaaggt cttttagaaa cgatgctgaa ggagttagaa gaaatttcgc
    60361 cacaaaagaa accagatcta ctggtttata ttcgtgtatc ctttgaaact atgttagcga
    60421 gaatcaaaaa aagaggccgg gaatatgaac agctagaaca agatcctgaa ctctattcct
    60481 actataaaga actcaatcga cgatacgaag agtggtatga acagtttgat atatgtccca
    60541 aaatagtgat cgatggggac aaatatgatt tcgtagccga tccggcatgt ggagaacaaa
    60601 tcgtacaaga aatcaagatg cgggcaaaga aaatgacaga agaggcagat gcccagtcga
    60661 tccgctacac tacaaataac ccgaaagcaa agggaatctt tacaactaaa taatataaaa
    60721 actaagcaaa ttgctcactc gcagatattt ctctgtgagc agcatcttgc ttagtttttt
    60781 tgattcattg agatcaaata gtgaaccgat tcgtatgata aaattgccat aaatgaaatg
    60841 tctgagtgag ggccatgatc aaaaataaaa tggaaacgat caaaatcgaa tgggcgccaa
    60901 acagatgatg acagaaaaca aggacaatag ctcctgcgat aaaggcaaga acgacactta
    60961 gatagtggaa aaatcgatca aaatctgctt tttctttaga aatacaataa tcatataagt
    61021 tcgttgctac cccttttaaa tttccagttg taaataaatt agtgtaggca cgcccgttga
    61081 ttttatcaaa agctacccac tggatcgatg cagtaaaagc aatcaaaatc gttactaatg
    61141 cgggatgctc atggacgggt gtcaaactaa caaagaaaaa aatcactgct tcataatata
    61201 gaacaaacaa gcgccagaaa tgtttttctt tttttctaaa ataatcaatt agatactttg
    61261 ttagaagaat ccctaagaaa aagaaaaggg tagacaatac ttttttacca acacttagcc
    61321 attctccgtc aaatgcttgg atgatcgata gaatgatatt gccagtttga gcagaggcaa
    61381 aagcaccata ttggatataa gtataactgt ccatggctcc tccgataaat gtcagcaaaa
    61441 gtcctattgt acgcccctcg tgaaaggaag tctgcatttt tttcatcacc ttttatgaat
    61501 atttttgtct tccttatctt agcataaacg attgaaaaaa aggaaaaatt ttcagaaaac
    61561 taaaatcata tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat
    61621 agacaacgaa actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga
    61681 aggaaatcaa tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct
    61741 gggagaacaa gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta
    61801 cctatgaagc ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag
    61861 aaagtgaagg aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca
    61921 atcaagaaca aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag
    61981 gagctgggag cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa
    62041 aacaagtaga cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac
    62101 taaaaaaacg agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc
    62161 atagtatctt ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc
    62221 aaggaataca aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac
    62281 agctgatacg taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt
    62341 ctcggttaga ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta
    62401 aagaaaaata tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg
    62461 gattgctcac ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg
    62521 tactccaaaa tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa
    62581 aatatgatta tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg
    62641 tgaaacggat gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga
    62701 gtatctatgg attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc
    62761 ctaccaaaac tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag
    62821 caaatagatt gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac
    62881 aaaaagaagc aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt
    62941 ggctaatcaa acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca
    63001 tattataccg atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata
    63061 ttccttttgt actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc
    63121 aacatttcta tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc
    63181 aggcctacca gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat
    63241 gcgatcaata cttttctcct aaatatcaaa atatatccgt tgcagattat attttgtcga
    63301 taagccccaa tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta
    63361 aaacagacca gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta
    63421 tagctaagga aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg
    63481 ctactttcag aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa
    63541 aaacccgctt gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc
    63601 acaaaagtaa aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt
    63661 tgccttataa cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac
    63721 cagtaacgac gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa
    63781 atcgtctgta tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat
    63841 ttatcaaaga aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca
    63901 gaagtggtca gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt
    63961 gaaatttcag cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga
    64021 ggtgtgacaa gaattttgtc acacctccct tatttatttt gaaaatgaga atactattat
    64081 caaaatagag aatatataga aaaattctca ttattcaaac gtaaataatc agttattttg
    64141 atttttttta tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg
    64201 gattataatt aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt
    64261 cctgtagaaa agcaaaagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata
    64321 tttttgccga accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag
    64381 gagttcaaga tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta
    64441 acgaattaac tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac
    64501 gacctatttt gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt
    64561 catttgtccc tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata
    64621 acatcttaaa ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac
    64681 tgtgtaacaa gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt
    64741 gttgacttag aagaacgcga tctaagtaca gaagaagcag aagaattatg cagttttgaa
    64801 gaagtgcaag cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt
    64861 gacctttcct gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc
    64921 ctcagacgtc ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg
    64981 atggaacagc ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt
    65041 tcatacagtt gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac
    65101 tttatttttc tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg
    65161 ggatatagag ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt
    65221 gttttaagta gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca
    65281 tgttagaaat gacatgataa gataactgag ttgttaaaaa agaaccactt gtgaagcttg
    65341 attctatcga aaaaaacttc aaaaaagttc ttgacaaata acaaatgata agttatgata
    65401 aatgagttgc tgtaagattt cgaaaaacga aatc
//
LOCUS       DAZUMV010000004        52600 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000004 DAZUMV010000000
VERSION     DAZUMV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.004"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..1119
                     /locus_tag="ACG8FK_000241"
     CDS             118..1119
                     /locus_tag="ACG8FK_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS5095882.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            1138..2157
                     /locus_tag="ACG8FK_000242"
     CDS             1138..2157
                     /locus_tag="ACG8FK_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS5095883.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2259..2975)
                     /locus_tag="ACG8FK_000243"
     CDS             complement(2259..2975)
                     /locus_tag="ACG8FK_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5095884.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2944..3729)
                     /locus_tag="ACG8FK_000244"
     CDS             complement(2944..3729)
                     /locus_tag="ACG8FK_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5095885.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3720..4469)
                     /locus_tag="ACG8FK_000245"
     CDS             complement(3720..4469)
                     /locus_tag="ACG8FK_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5095886.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4767..5291
                     /locus_tag="ACG8FK_000246"
     CDS             4767..5291
                     /locus_tag="ACG8FK_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS5095887.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5307..6806
                     /locus_tag="ACG8FK_000247"
     CDS             5307..6806
                     /locus_tag="ACG8FK_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS5095888.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6894..7496)
                     /locus_tag="ACG8FK_000248"
     CDS             complement(6894..7496)
                     /locus_tag="ACG8FK_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5095889.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7600..8556
                     /locus_tag="ACG8FK_000249"
     CDS             7600..8556
                     /locus_tag="ACG8FK_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS5095890.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8861..9817)
                     /locus_tag="ACG8FK_000250"
     CDS             complement(8861..9817)
                     /locus_tag="ACG8FK_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5095891.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9991..10551
                     /locus_tag="ACG8FK_000251"
     CDS             9991..10551
                     /locus_tag="ACG8FK_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095892.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10544..11368
                     /locus_tag="ACG8FK_000252"
     CDS             10544..11368
                     /locus_tag="ACG8FK_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS5095893.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11378..11995
                     /locus_tag="ACG8FK_000253"
     CDS             11378..11995
                     /locus_tag="ACG8FK_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5095894.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12013..12696
                     /locus_tag="ACG8FK_000254"
     CDS             12013..12696
                     /locus_tag="ACG8FK_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS5095895.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13379..14662
                     /locus_tag="ACG8FK_000255"
     CDS             13379..14662
                     /locus_tag="ACG8FK_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5095896.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14678..15565
                     /locus_tag="ACG8FK_000256"
     CDS             14678..15565
                     /locus_tag="ACG8FK_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5095897.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15582..16418
                     /locus_tag="ACG8FK_000257"
     CDS             15582..16418
                     /locus_tag="ACG8FK_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5095898.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16421..17044
                     /locus_tag="ACG8FK_000258"
     CDS             16421..17044
                     /locus_tag="ACG8FK_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095899.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17041..18333
                     /locus_tag="ACG8FK_000259"
     CDS             17041..18333
                     /locus_tag="ACG8FK_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5095900.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18692..18789
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18905..19486
                     /locus_tag="ACG8FK_000260"
     CDS             18905..19486
                     /locus_tag="ACG8FK_000260"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS5095901.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19489..20796
                     /locus_tag="ACG8FK_000261"
     CDS             19489..20796
                     /locus_tag="ACG8FK_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS5095902.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21212..21700
                     /gene="purE"
                     /locus_tag="ACG8FK_000262"
     CDS             21212..21700
                     /gene="purE"
                     /locus_tag="ACG8FK_000262"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS5095903.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21693..22817
                     /gene="purK"
                     /locus_tag="ACG8FK_000263"
     CDS             21693..22817
                     /gene="purK"
                     /locus_tag="ACG8FK_000263"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS5095904.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22830..24125
                     /gene="purB"
                     /locus_tag="ACG8FK_000264"
     CDS             22830..24125
                     /gene="purB"
                     /locus_tag="ACG8FK_000264"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS5095905.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24305..24682)
                     /locus_tag="ACG8FK_000265"
     CDS             complement(24305..24682)
                     /locus_tag="ACG8FK_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095906.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24938..25666
                     /gene="purC"
                     /locus_tag="ACG8FK_000266"
     CDS             24938..25666
                     /gene="purC"
                     /locus_tag="ACG8FK_000266"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS5095907.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25666..25920
                     /gene="purS"
                     /locus_tag="ACG8FK_000267"
     CDS             25666..25920
                     /gene="purS"
                     /locus_tag="ACG8FK_000267"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS5095908.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25922..26593
                     /gene="purQ"
                     /locus_tag="ACG8FK_000268"
     CDS             25922..26593
                     /gene="purQ"
                     /locus_tag="ACG8FK_000268"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS5095909.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26594..28816
                     /gene="purL"
                     /locus_tag="ACG8FK_000269"
     CDS             26594..28816
                     /gene="purL"
                     /locus_tag="ACG8FK_000269"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS5095910.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28801..30240
                     /gene="purF"
                     /locus_tag="ACG8FK_000270"
     CDS             28801..30240
                     /gene="purF"
                     /locus_tag="ACG8FK_000270"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS5095911.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30263..31315
                     /gene="purM"
                     /locus_tag="ACG8FK_000271"
     CDS             30263..31315
                     /gene="purM"
                     /locus_tag="ACG8FK_000271"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS5095912.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31312..31890
                     /gene="purN"
                     /locus_tag="ACG8FK_000272"
     CDS             31312..31890
                     /gene="purN"
                     /locus_tag="ACG8FK_000272"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS5095913.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31887..33422
                     /gene="purH"
                     /locus_tag="ACG8FK_000273"
     CDS             31887..33422
                     /gene="purH"
                     /locus_tag="ACG8FK_000273"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS5095914.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33438..34712
                     /gene="purD"
                     /locus_tag="ACG8FK_000274"
     CDS             33438..34712
                     /gene="purD"
                     /locus_tag="ACG8FK_000274"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS5095915.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34834..35748
                     /locus_tag="ACG8FK_000275"
     CDS             34834..35748
                     /locus_tag="ACG8FK_000275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS5095916.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35881..37446)
                     /locus_tag="ACG8FK_000276"
     CDS             complement(35881..37446)
                     /locus_tag="ACG8FK_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5095917.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37439..38890)
                     /locus_tag="ACG8FK_000277"
     CDS             complement(37439..38890)
                     /locus_tag="ACG8FK_000277"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5095918.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38904..39920)
                     /locus_tag="ACG8FK_000278"
     CDS             complement(38904..39920)
                     /locus_tag="ACG8FK_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5095919.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            40140..41228
                     /gene="chvE"
                     /locus_tag="ACG8FK_000279"
     CDS             40140..41228
                     /gene="chvE"
                     /locus_tag="ACG8FK_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5095920.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41282..42808
                     /gene="mmsA"
                     /locus_tag="ACG8FK_000280"
     CDS             41282..42808
                     /gene="mmsA"
                     /locus_tag="ACG8FK_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5095921.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42810..44015
                     /gene="mmsB"
                     /locus_tag="ACG8FK_000281"
     CDS             42810..44015
                     /gene="mmsB"
                     /locus_tag="ACG8FK_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS5095922.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44273..45250
                     /gene="guaC"
                     /locus_tag="ACG8FK_000282"
     CDS             44273..45250
                     /gene="guaC"
                     /locus_tag="ACG8FK_000282"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS5095923.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45514..46938
                     /locus_tag="ACG8FK_000283"
     CDS             45514..46938
                     /locus_tag="ACG8FK_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5095924.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(46980..47237)
                     /locus_tag="ACG8FK_000284"
     CDS             complement(46980..47237)
                     /locus_tag="ACG8FK_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095925.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            47399..48508
                     /gene="pdhA"
                     /locus_tag="ACG8FK_000285"
     CDS             47399..48508
                     /gene="pdhA"
                     /locus_tag="ACG8FK_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS5095926.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48511..49488
                     /locus_tag="ACG8FK_000286"
     CDS             48511..49488
                     /locus_tag="ACG8FK_000286"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS5095927.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49513..51156
                     /locus_tag="ACG8FK_000287"
     CDS             49513..51156
                     /locus_tag="ACG8FK_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS5095928.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            51164..52570
                     /gene="lpdA"
                     /locus_tag="ACG8FK_000288"
     CDS             51164..52570
                     /gene="lpdA"
                     /locus_tag="ACG8FK_000288"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS5095929.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT        16823 a         9104 c        11648 g        15025 t
ORIGIN      
        1 tattagcaaa ataaaagctt tttttattgt gaataaatga acataattag gaggagaaag
       61 atgaacttat ttgacatttt tcgatcgact ttgacagata tgtcgattgt cagtgcaatc
      121 acttcaacag tttttattat attattaggg tttgtttgta gaaaaaaggg gattttttct
      181 gaatctgttg gaaaaattct atcgaaagtc gtcttaacag tggctttgcc agcactggca
      241 tttcgagctt tcatgcaaga tatcaataca gaattactga tgcaaggaat caatgtcttg
      301 atttggggat tagttattta tattattctt attctagttt caaaaccttt tttcttaagc
      361 tataaagggg ataagcaaac aacgttgcgt gtcctgacga tctttggttc tacgaccttt
      421 ttcggaacgc ctatcgtcag tgcgatttat ggtcctgtcg gcgtgatgta ttcctcaatt
      481 ttcaatattg gctaccgaat ctttttgtat tcttatggat acataaaaat gagtggcttg
      541 aagatggaag ctaaaaatat caaagcgatg ttcgtcaatc caatcattat cgctactttt
      601 gccggccttt tcatttggct gttccaaggg tatctgccac aagtgtcagt tacaggtgca
      661 gaaggagaga taagccaagt agcgtttttg cgaattgacc agactgcacc gtggctattt
      721 cagccgatgg attatttagc tggtttaagt tcaccattgg cgtggctagc aatcggcgca
      781 acattaggtg aagtaagctt caaaaaagct gcatctgaaa aaacttcatg gtattacagt
      841 ctagtcaaag tggtatttgt acctgcattg aatatcgtga ttctagcagt tttgacggca
      901 acgaatattc taccagtcaa ttttgaagca ctggcaacaa tcgttatcat gatggctaca
      961 ccagctgcaa cagtggcagc agcctatgct atcagttttg atagggaggc acttttagcc
     1021 tccaatgcat ccttactgtc aactattttt gctgtggtac tgacacctgt atggatcgtt
     1081 gtattacaaa tgattcatgg cataggttta ttttcataag aaatggagga agaaagtatg
     1141 agaatcgcat gttacggtgt tcgtccgaac gagatagatt tttttgaaaa attgaatatt
     1201 taccattatg atctcagtct ttatgaagaa ctgttgacac atgacaatat cgtgtcagca
     1261 aaggaccatg atgctgtatt gttgagggga aattgtatag cagatgagac caatcttgca
     1321 aaaatgcagg aatatgggat tcgttatgtt tttacacgga ctgtgggagt caatcatatc
     1381 gatctacaag cggcagctga ttttggtatg acagtagccc gtgtaccatc gtattcgcca
     1441 aatgcgatag cagagttatc tttgactttt gccatgatgc ttttgagaaa tactgcgtat
     1501 acgacgatcc gaacatcttt caaagatttt cgagtggatg agcaaatgtt tagtcgtgaa
     1561 atcagaaatt gcacagtagg gatcatagga acaggcagga tagggttgac ggaagctgtt
     1621 ttgtttaagg gactaggagc caaagtgatc ggctacgata ttcatcagac agaagctgct
     1681 aaaaaagtat tgacgttcca ctcgcttgaa gagttattag ccaaaagcga tattgtcagt
     1741 attcatgtac ctcatatccc aggtgaaaac gatcaaatga tcaatgaagg atttttagct
     1801 caaatgaaaa aaggtagcat cttgattaat acagcccgtg gagagcttca agataatgaa
     1861 gctattttaa gagctttgaa aagcaatcat ttagctggtt ttgcaaccga tgtttttgca
     1921 aatgaaacag aggttttttt ccgatccttc aagccatggg agacgattcc agaccctgct
     1981 atccagcaat tagtagaaat gtaccctaga gttcttgtaa ccccgcatgt aggttcaaat
     2041 acagatgagg cattatcaaa tatgatttcg acaagtttcg agaactttcg tgaaataata
     2101 gaaactggaa aaacgaaaaa tgaggtatct cttcctaaag cccgtcaatt aagataagaa
     2161 aacatggata ttcgtcaata aaatgtttaa aaatagtcca agtaaacagt tgttacttct
     2221 gtatacttgg actattttgt atgggataat tcgatgactc aacagtaatc tgtgagcttt
     2281 ttccgcccat tctttgtcaa ttcataacag gttagcggcc ggccgatttc caaatatttg
     2341 attctttcgt tcaatatatc ttgttctact aaaaaattca aatatttctt gacggatata
     2401 cgtgacagtg agcttttttg agccagttct tttgttgaaa aaatagtatc ttcttttaga
     2461 ataaatgcac ttattttttt taaagtcagt tttgacagac ctttaggcaa atcgatgaat
     2521 gatggtattt cgtgaatggg agtggtgtgg ttgaatactt gatcgatcaa tttctgatcg
     2581 gctttttcag tcgtttggat tgtctgagag aaagcaagat acttttcgat cgccagctga
     2641 aaacgttgca aagtgaacgg tttgatcaaa taatctaagg caccgtagcg gatcacattc
     2701 tttatggttt cgatatcatc tgcagcagtg atcaaaatca ctgggatatc atttccttgc
     2761 tgtctaagcc aagataaaaa atggatacca ctttcgccag gcatgtatac atcaagcagc
     2821 agcagatctg cgtttttttg tctgaaatat ttttttcctt cttctacatt tctgaaactt
     2881 tcgattacgt tcatattagg gatgctttcc acaaaagtac gattcaattc tgcaaccatt
     2941 ggatcatctt cgattatcag gacatttgtc atcattttga tcttcctttt ttggtaattc
     3001 tagatagacg attgtaccgc catttgtttg tttggaaaat tctaaaaatc caccgtggcg
     3061 tttgacgatt gaatggatga gattcaatcc aaacccacga ttgattccct tcgtcgaaaa
     3121 acctagcgta aatatttttt ctgagatttc ttctgatatc ccgacaccgt catcgcagac
     3181 ggtcacgatg aatacttcag cttctgtgtc atagtttaaa tgaatccgga cctgtccgtt
     3241 tctttcatgt ttttgtagca cggcttcttt tgcattatca agtaagttcc ctaacacttg
     3301 caaaagtgaa tggatggagt catccatctc cattttaggg ataaaagaat cgctttctaa
     3361 cagcatttca acagattgtt ctttggcttc gtttattttg cctaataaaa aaccggcaat
     3421 tgcaggtact ttgatcagac ttgagacttg gccaatctct tttttataat tttgatttag
     3481 ctgctggata aaatgggtga cttcttcata cttcttcaaa tcaatcaaac cagaaatgac
     3541 atgaagttta ttcatgaatt catgggtttg agctctcatg gagtttatat attgtttcgt
     3601 ccccccaagc tcctccatta atttcattac ttctgatcga tttcgtaaag ttgcgactgc
     3661 tcctaaaaaa cggtcattgc taaaaagagg tgtaacgcta gaaatagttt ctatcccatt
     3721 taaatacatg gattgagtcc gaacggtcgt ttgatgctca atcgtctctt taaacaaaac
     3781 gtcatataga ttatcactga tttttttcct acttcgaaat ccaatagctg ctctgtttgg
     3841 tagaaaatgt tttttgcagg tgtattgatc aagaggatag ttttatcaga agagatggct
     3901 aagattcctt catctacttc attttcgata atcgtttttt cacttaactg ttctgctatt
     3961 tcttgtggtt caagtccaag cagtactttt ttcatttttt gggcaagaaa aacggcgcct
     4021 atgatgccta cgctaagtcc aataatcaat gaaaaaacaa tcattttttg tccatctttg
     4081 acttgattgt caatcgtgtt gtttgttaag ccaacacaaa ctgcaccaat aatctcttga
     4141 tcataataaa tagggacgaa atagcggaag ccgaacccta acattccttc tttttcagag
     4201 aaaaattctt gtcctccaat cacttttttc acatcttcat gacttgagaa aggaagaccg
     4261 atgtaacttg ggtctgcgtg agaatatcga atgccttcag ggtccatgac aacgataaaa
     4321 tctaaattcg atgcaatcat gatttcattt gttattcctt gaatcgcatg tgtcgggtca
     4381 ccagtctgca gctttttttg gatgagttta tcattggcaa cgattttagc aatgctagag
     4441 attttatctg acacactaga tcggttcatc gaaagaataa aattcctgat caatatggca
     4501 gaaacaatca aagaaacgaa tgtggagaaa caaacgacaa ggatgatcgt gttctggagc
     4561 tttaatggtt ttttcttaaa aaaaggaaac ataggctacc tcgaaagaat ttattgtgaa
     4621 aattttaaca tgaaaacaaa aatttaacaa attgtcaacg ataaacacaa ccttcctata
     4681 aaaaacacca ctgagtcggt aaaagaagct atacagaatt ttcaaacatt ggtatgatag
     4741 acgtagaaat ggaaggaggc gtgtatatga tagggggaat cgcacttgag acgatttatt
     4801 ggtatacatt gctgatttgt gcaggattgg cagtcctatt gattattttt ggagatgtat
     4861 tcgattttga tggcccgctt gatccgatgc ttattatccc ttggttaact ttcacaagct
     4921 tatttggttt tctaggtgag cgtttaacag atcaatcatc tttattttta tttattgcta
     4981 gtggaggcat atcgatagtg ttggtatttt tgcttaattt ctatatattg atgccgatga
     5041 aacatgccga atcaacgatg tcgtcttctg aaaaaactct ggaaggacaa gtagctgttg
     5101 tgatcacacc gatccctgta cgaggtatgg gagaaattca gttgaaaagt gttaccggtt
     5161 ccattagtcg tccagcttgt ttttacagcc cccaagaaca gacagctgag cggggaacga
     5221 aagtattgat cattgagatc aaggaaagaa tctgttatgt catgccttat gaaagcatga
     5281 taaaagctta aaggagcgtg tgttaaatgg aaataccagt tgtttttatc gcgatcgcaa
     5341 tcgcagcttt gattttactg atgttgctga tcgtctttgt agcgaagtac cagacagcaa
     5401 aaccggatga agcgttgatc atcagtggaa gttatttagg aagcaaaaat gtgcacattg
     5461 atgaaagcaa taacaaaatc aaaattgtgc gtggaggtgg cgcattcgtc ttgccagttt
     5521 ttcaacgttc gaatcggatc agtctattat cgagtaaatt ggacgtctcc acccctgaag
     5581 tgtatacgga acaaggcgta ccggtgatgt gtgatggtac ttcgatcatc aaaatcggtt
     5641 catctgtaga agagattgct acagcagcag aacagttttt aggcaagaca agagaagaac
     5701 tggaaaatga agcaagagaa gtattagaag gacacttgcg ctcgattctt ggttctatga
     5761 ctgtagaaga aatctatcaa aatcgcgata agttcagtca aagcgtacaa gaagtcgctt
     5821 ctgtagacct tgcaaaaatg gggttgatca tcgtttcttt cactatcaaa gaggtcagag
     5881 acaaaaacgg ttatcttgac tctttaggga aaccaagaat cgcccaagtc aaaagagatg
     5941 cagatatagc agaagcggaa gctctaaaag aaacgcgtat caaaaaagct caagcagaaa
     6001 aagaatcaca agcagctgaa ttacagcgac aaacagagat cgcagaatca ttaaaagaaa
     6061 aagaactgaa attagcaacc tacaaacaag aacaagatgt tgctaaagca aaagcagacc
     6121 aagcctacaa tttagaaagt gcccgagcac aacaacaagt aatcgaacag gaaatgcaga
     6181 ttaaaatcgt agaacgtcaa aaacaaatcg aactggaaga aaaagaaatc acacgtcgtg
     6241 aaaagcaata tgattcggaa gtgaagaaaa aagcagatgc cgatcggtac gctaaagaac
     6301 aagaagcaca agctcaaaaa gtcaaagaag tgactgaagc cgaagcagaa cgattccgtg
     6361 tggaagcact ggcagaagcg gaagccaata agacacgttt agctggacaa gcagaagccg
     6421 aagcagcact agcaaaagga aaagcagaag ccgaagcaaa acaaaagatc gccaatgcct
     6481 tcaaggaata tggcgaagca gctgtcttga gtatggttat cgatatgcta ccgcaattga
     6541 tgcgcgaagc agcacagccg ctaggaaata tcgaaaaaat ctctgttgtc gatacaggaa
     6601 gcagttcagg agaaaccagt ggagcgaacc gagtgacaaa ttatgccaca aatttgcttt
     6661 cttctactca agaaacacta aaagcgacaa caggacttga cttgaaggaa atcgtagaga
     6721 atttgtcgac acgtgggact actagccaag taaatatgta tgaagcgcca aaagaggcag
     6781 aacaaaactc gatgaaggaa gaataaagta gtacacttca ccgttcaatc aaaaatggag
     6841 gaagactttt taaagttctt ctctccattt ttttatttta ttgcaaaaga aaattagtcc
     6901 tcgctatttt tcttttgttt ttggatctta gcttctatct cttgtaaatt tgttcttagc
     6961 aatcgttctt tcttttggag ttcgaccaaa tcttctgata gttcttcctt cttttctgtt
     7021 tcaggaggac gaagaaagaa attgataccg attgcaatga atgtccctat aaaagtatct
     7081 agtacacgtt ggatagcata caaagcggat tcgccttgag ggatgctcaa cgcaatcagc
     7141 aacaacgtag ctattgcgga aatgataccg gaattattgt taataccatc tgacacgacg
     7201 atcacaataa taactagaat aggaagcaag atcaattcta ccaaaaaatc attgtgaaaa
     7261 tagcttttga caaaaaaata aatgatagct aatgtaccac ctaatgtatt gccgatgatc
     7321 ctagactttc caaaagaaac ggttgtagtc aagtcctgac gcaaagaaaa aacagcagat
     7381 aatgcagcaa tcatcgggat tcctcgttga gtcaaatgaa aaaacaagat acataaaaaa
     7441 acagctaagg cacttttgat cgtacgcata cctagtcgaa aacggccaaa ttgcatagag
     7501 attctccttt ttggtctttt tactatcata cctaaacttt tgtctgatag ctagtttttc
     7561 attattcaag tataattaaa ataaaaaagg gggaagtaaa tgcgtatagg aatgcgaaca
     7621 gtcaaaacag tgatcagtgt cttcttttcg atggtcacag cgggtagctt atcgctgttg
     7681 tattggccag ctgcagggat cattgcatta ttaagtgtag gaaataccaa gcgctctaca
     7741 ttgcgtacgg gcatgaatcg aatcgcagcc tttatattag ctacggcaac agcactgttc
     7801 agtttttttg ctgttgggta tactattttt ggcttcagtc tttttttatt cctgttcatc
     7861 ccgcttgcct ctcgcttcaa attaaccgaa gggatagtag taaattcggt tttggtcact
     7921 cattatttag tggaggaaaa catgtcttgg cagctgatcg gcaatgaagc gggattaatg
     7981 gcgattggat tagtatttgc tttgcttgca aatgtttaca tgccagatcg tacgaagcag
     8041 ctgaaggaaa accaaataca gatcgaaaaa gaatttcgaa atattttgag acaaatggca
     8101 gaatttcttt tatcagaaaa taaagaagat gtgcagatca aatgcgaaca tttactgaca
     8161 ttcattcgcg aaagccaaga agatgccaga gaacatcaag aaaactactg gctcagacag
     8221 ccgctatact acgaaactta cttctctatg agaagagcac aggccaatgt gattaaagac
     8281 atgctggaaa atctagagag aatccagcaa ccagcttatt atggaaaaca tatctatggt
     8341 ttattgattt atactgcaga aacattttca gagtcaaatg atggcagaca aattttaaca
     8401 cgtattgaag aagtttatgt attgtatcgg caaatgcctc tgccgactag tcgccctgaa
     8461 ttcgaggatc gagcagaatt gttccagttt ttgcagtctt ttaaaagttt tattgaaatc
     8521 aaggcagaat tctcccaaca aaaaatccaa aaatagaggt tgtgaattca gctaatattc
     8581 aggtcaaaaa gctgatttca caacctcttc tttaaacggt gttctcctgt caactcctcg
     8641 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ctccttgtga
     8701 ctcggagctg aacggtaggc tcacaacctc tttcttaaac ggtgttcaaa agctttcctc
     8761 tgcggtaata agtccagcca aacgaaaaat aggaggagct atttttgttt ggctgttctt
     8821 attacttgat gcagaacagc tttttcacaa ccttttaaaa ttacatattt ttccatagaa
     8881 cttgcatttg atccaagatt tcgatgacat cttttgtcaa gaagagagag cgattgggca
     8941 agtcaccttc aatcatgtgt accatattct ttatttcgta attcattgct tcagatgtat
     9001 tcccgctttc gatgaattcc tttgtaccat ctgtaaataa gatctccgca cgatcagcac
     9061 gcgggtaatc agcaatcgtc acataaccgt tctcaaaggc aacgataccg acttttggca
     9121 tttttgcctg aaaagtcaag ctgacagcag caagttcatt ttctttgtta cgcaagatag
     9181 tgacggattg ttcgtccacg cctgtttcaa aaggaaccat tgtagaggca actacttcag
     9241 gttgagagct caaaaagaaa cgagcgaaag atacagcata cgtaccgata tccaacagag
     9301 ctcctccagc taattctgga ttgaagaacc ggtttttagg gtctggttct ttgtaactgc
     9361 cgaaaggtgc ttggatcatt tttaaagccc ccagttttcc tgtatccatt attgagcgta
     9421 actgctgata aagtggcatg ttgaagattg tcatcgcttc tgctaagatc aaattctttt
     9481 cttctgctaa gcgcatagcg tcagccagtt cttttttatt catcgtgatg gctttttcac
     9541 atagaacgtg tttgcctgct tccaatgctg ccaagatatg gtcgatatgc tgtctatttg
     9601 gtacagcaat gtacacgatg tcgatttcag catcgtctaa catttcttga tagcttccgt
     9661 aagcttttgg aatattgtaa cggtgagcga agttttccgc tttttctgaa gttctggaag
     9721 caactgctgt tagttcgctc gtctcttgat tgaagctttc ggcaaactca tgtgcgatgg
     9781 taccaagacc aacgattccc cattgatatt ttttcatctg aaaacgctcc ttcatagttg
     9841 gattgagttt cttgtctgtc tattattata acaaaaatca cgaagaataa agaggagagg
     9901 acttgaaaca ttaaaaaaaa tgaaagcagc gatttaagag gtgctttttg atcaaaactt
     9961 aagtagaata gttaaaagga ggggataaac gtgcatcaag tatatattga tcttattatg
    10021 agtgtattat tagaacagtt tgaaactgaa aaaaattttt tagatgaata cttgctgatc
    10081 agtccagaac aatggcaaaa atggaaagat ggcgagaaaa atctgccatc tgaagcgatg
    10141 cagaaagtaa aaaatctttt cactgattat gaatggatgc taacgcaaaa gattttgcgt
    10201 caaacgatta tttttcccga aaaacgaaac gttgccgttg cagagtacaa gcaaatcaaa
    10261 acacgcatcg cgcaaaaatg gcttagcagc aaagcagcaa aagtcgagct gatccctttg
    10321 aaaaataatc agcaggctgg tagatacctt gacttgaaag taagtattca atatgatgaa
    10381 tggggctatg atgatatttt gaattttcgt ctgccagcag atgttcaaaa acagatcgaa
    10441 ggagaaaaaa tcgaacttct cgattgggtg aatgaaaatc tggaagaaac ttacgttcat
    10501 gaggcgaatg gggaacgtga agaacaacga aaggattttt caagtgaata aatttttagg
    10561 atttggagca gttgtgatta tgatcgcagc aatgggctat gttattttta gcgatgatga
    10621 ttcgcatgtt caagccggca atcaagcagt cgaacgaaca agcaccaaaa caactgctag
    10681 taaagaaaag aacagtcaaa caaccagcag caaggtaaag gacaaagaaa caaaagatcg
    10741 tacgtccaaa caattgccag atgttaaaac agacgattgg aagctagttt tggttggtcc
    10801 agatcacaaa ttagaaaaag aaattgatga gtctactcaa ttggcatcat taagtaacgg
    10861 atacttgatc gacaaacgca ttgctccaga atatgaagca tttgccaagg ctgctgaagc
    10921 agctggattt ccattagtga tggtctcagc ttaccggtct gtatcatccc aacaacaagt
    10981 tttcgaacaa aatgtccaag atgtgatgag tcgtcaaggt gtatcagaag aggaggcgac
    11041 aaagattact aaagaaacga ttacggagcc aggatacagc gaacaccaca caggtctggc
    11101 agtcgatgtc gtcgatcaaa actggtacaa cagttatcct tctactgtat tggatgcaag
    11161 ttacggagat cagcctggag ctaaatggat cgcagataat gctgcgcagt atggatttat
    11221 cgttcgttat ccaaaaggac acgaagatat aactaagatc acatatgaac cttggcactt
    11281 acgttatgtc ggtaaagaaa acgctgagta tatcaccaaa caccaattga cgctagaaga
    11341 atatctgaat gaattaaatg aaaagtagga attgatcatg gcgaaaaaaa agtggaaaaa
    11401 gcaaaagcgc aagaatatcc gttggccagc agtggttgcc ggtgtagcag tgatactctt
    11461 agcctttgtt ttttccttga agaatctgtc ttctcctttt actgataatc agcaagaaaa
    11521 tgaagcgcaa tcccatcagc aatttattga acggcttgtt ccgcatgccc aagaactaca
    11581 agatggttat ggagtgcttc ctagtatcat tttaggacaa gctatattag aatcaaattg
    11641 gggaaaaagt accttggcta gcaaatacaa taatcttttc gggatcaagg cttatgggga
    11701 tcagaagaaa gtaagcctgg aaacaaagga atttgttaac gaagaatgga taactatcca
    11761 aggagacttc aaagtgtatg attcatggga acagtccatg gatgaccata cacaactgtt
    11821 cgtccaagga gtagattgga atcctgcgct gtatgaaaaa gtgattacgg ccaccaacta
    11881 tcaggaagcc gcacaagcat tgcaagatgc aggttatgcg acagaccccg gttatgcgca
    11941 aaaaatcatc caggtcatcg agacgtatca attgaatcag tatgaccgga gatagcaaag
    12001 gagaagtggc tgatggcaga acaatttacg ccagaaatca tgacggaatt gagaaaagat
    12061 atcgtaagag taccggaaat cattttagaa gcaagcggga tcaagatatt tggaaaattg
    12121 atccgttcga tcatctttac tacggatatt gcgatcatac gtaatacaaa tgcgaatgca
    12181 gtaattgctg tgtatccttt tacacctcat ccagcgatta ctaaaagcat tattgaggca
    12241 gctgacattc cagttttctc cggtgtaggt ggtggtttga cacaaggtgc tcgttcttcc
    12301 tacatgagca tgttcgcaga agcgcaaggt tcgttagggg tcgttttgaa cggtcctacg
    12361 cctttagaga cagtaaaaga agtttgtcga gtagttgata ttccagttat cagtacggta
    12421 acgtcgaaat atacagaaat agatgaaaag ttagcagctg gtatttcgat tatcaatatc
    12481 agtgcaggta aagaaactgc agaagcagta cgccattttc gtaaaaaata tcctgaactt
    12541 cctatcattg cgacaggtgg tccaacggaa gaaagtattg ctgagaccat tcaagcagga
    12601 gcaaatgcca tcacatatac gccaccaagt agcggagagc tttttagtcg taaaatggaa
    12661 aaatatcggg aaactgagaa aaagaatcat gaataacagg tgagaatcaa aagacgagga
    12721 agagcagata gtggctcttc ctcgtctttt gtactccagt actatttgtt cagaattcaa
    12781 aaaaaagaga aatttttttg tttttttacc tgtaatgtac ataagaagag ttaaaacctt
    12841 ttgttattat tagcattcta acaaaaaaat gtcttgattt attgaagcgc tttctataga
    12901 atgaaaaacg aataaaaact aaaaaaggca cccgacaatt tagtagcagc taaattgtcg
    12961 gggcttgaaa agtcagcggg aactgaccaa tcaagttgcc atagtcaatg cttgtgcatc
    13021 gacgtgtata ttgtacctaa tttcatacag aattactagt tatttttctg ttttttaggg
    13081 aagagcgcac acgaagaagc atgcttattt agctatgtca aggatattgg gcagttgtct
    13141 gatatccttt tttctttgcg taattatcct cccatcattg aagcaagaag tgtttttagc
    13201 gatgaaagaa gaatcagcac tatctaattt ttcttgaaag tattgagttg ttcatcctag
    13261 gcagaacgat aaggcaagtc ttggttttta ttccccttga aaaagatcgt ttaataaata
    13321 tgttctgtat aaaaatacag tatattgaaa aataacaatg gaagtagagg tgcagagaat
    13381 gaagaaaaga atgagaatca tcttagcttt aagtgttatg ttattcttct tgacggcttg
    13441 tgggcaagaa gatgacaaaa aaatcacgct gaacatggct tggtggggtt cgcaagtccg
    13501 acacgatgca acagtaaaag taatcgagct ttatgaagaa aaaaatcccc atgtgacgat
    13561 cgattatgaa ttttatgatt atgaaggata ttacacaaaa ttaggtacac tagcagcatc
    13621 agatgatatc tgggacattt tccaactgaa caatcaattt cctcaataca tcaatcagat
    13681 tgaaccatta gatgaatatg tggagcaagg gataattgat atttctaatg cagatgaatc
    13741 ctatttagca acgacgactt acgacgggca attagttgga ctgtctaacg gggtgaattc
    13801 gtttgcgatt gcttacaata aaaatatatt tgaacaatta aatatccctg aaccagcttc
    13861 aaattggaca tgggatgaat ttgaacaaat cagctatcaa atcactgaag aattaggaat
    13921 ctatgctatt tcaagattcg aagattttat agctggctgt attattcaaa taccacaagt
    13981 tgagaaagga ttgaattttt ataatcggga agatctatct aacagcttgg gatttgaaaa
    14041 tccagactat ctaacagatt ttttccagct gcgtaaaaat ctggtagaca aaggggccca
    14101 tccagaacca ggtggtggag ctgcaagtac gacggatctg tcttttcagg cggttaggga
    14161 tagagaagcg gcaatggtgt ttctatcaag taaccagttg acagaaatat tgtcaggggc
    14221 tcctgaagat atggagattc agttgattaa tccacctcgc agaaaagcag atggagaatc
    14281 tggtatccct ttacgttctt cgcaaatgct gagtatggca aaaagctcag cccataaaga
    14341 agaagctgct aagtttattg atttctttca aaacagtgaa gaagccaatg aaattttaaa
    14401 aggagaacgt ggtgtaccaa tcatgtcgaa tattcgagaa cagctcgctc gtgcagatga
    14461 tgcaactttg caagcgacat ttgattattt agaaatgatt tccgatatgg acaacggcga
    14521 agtgaatgtc tttgatagtg ctttgaatcc ggaaattcaa gaccaataca atcttttagt
    14581 tgaaaaagtc atttatgaag aagtaacagc agaagaagca gcagagagtc tttatgagtt
    14641 tgcgtcaaac attctaggat agcagcagga ggaaggcatg aaaaaagaaa aactaaaacg
    14701 gttcttggat caagacaatg tggtcggtta cgtatttgct tctccattca tcctaggatt
    14761 tctattgata acagtgatac cgatgatctt gtcactttat tattcgttca ccgattatcg
    14821 tttgggaaat ccagtctcat ggatcggctt ggataattat ataaagatgc tgagtgatgc
    14881 ccgttttttg aactccatcc gagtcacttt tctctatgta tttgcatctg tacctgtgaa
    14941 actgatcttt ggtctattga ttgcttatct tttaacgagg aatatcaaag gaacgacttt
    15001 ctttcgttct ttgtattatg tgcctagttt gatcggtggc agtatttcta tcgctctagt
    15061 ttggcgggag ttgttttcta aaaacgggtt agtgaatatt ttcctggaac gattaggcgc
    15121 acctgcgctt tcctggttta gcgatcaaaa actggtcatg atccctcttg ttttgatgtc
    15181 tgcttggcaa ttcggttcgg caatgatcat ctttgctgca ggtatcaaag agatcccagg
    15241 ctcgtattat gaagcggcag agatcgatgg agcgaacaaa tggcagacgt tttacaagat
    15301 cacgttacct cttctttcgc cgatcatttt gtataacttg gttatgcaga caatcgcggc
    15361 gtttatgact tttacgcagg catttatcat tacccaaggt ggtccgaatg atgccacgaa
    15421 cttttatgct ctttatgtgt ataaccaagc tttttctttt aattctatgg ggtatgctag
    15481 tgctatttcg tggatcatgc tgttgattat gggagctgtc acctttctga ttttcaaatc
    15541 atcaaaaaaa tgggtcgttg tgtgaatgag aggaggaaga aatgaataat acagtaacga
    15601 aatggtttca atacggtttt attttgctat taggtctagt catgatttat ccggtcttat
    15661 ggatgatcgc aggttctttt aaaacggatc aagagattat gagtggttcg ctgaatttga
    15721 ttccagagac ttttcaatgg gaaaattacg caaaaggatg ggcggggttt gctggaattt
    15781 cattttttac tttctttagg aattcgtttg tcatctctgc agtttcgacg atagggacag
    15841 ttctgagctc gacttgtatt gcttacgcat tatcgagaat cgatttcaaa ggaaaaagat
    15901 tctggttcgt cgtcatgtta gtaacgatga tgattcctgc acaagtgatt ttgattccgc
    15961 agtttattat ttacaatcgg ctgaatttgg ttgggacgta tgtgccgctg attttaccac
    16021 atttttttgg acaagcattt ttcatttacc agatcatgca attcatggtc agcatcccca
    16081 aagagttaga tgaatcagcg attattgacg gatgcagcaa gtatagtgtc tttaccaaaa
    16141 ttatttttcc tttgctgaaa ccttcgatca tcacgacgat catcatccag ttttattgga
    16201 aatgggacga cttcatggga ccattgattt atttaaacaa gccgagatct tacacggtat
    16261 cgattgccat caaattattt gcagacgctg gatctagtac gacttacgcg agtatgtttg
    16321 cgatgtctac gttgtcgctg ctaccggttt ttctgatttt cttattcttc aataaatatc
    16381 ttgtccaggg gatcagtaca agcggtctga agggctgaat atgaaaaagc caatagaaga
    16441 gatgtttctt tacaagctca actactatgt ctgtcggatt tttttaacca gtcttatcag
    16501 taatgctctt ttcgctttag ttctaggatg tttgttcact ttaggattag tgctgtctgc
    16561 tcgtgttgtc tatctgattt attttttgat gatgtatcct tcttattatc tatctgtcca
    16621 ttacttggca gaaaaaaagg tatgttggag gaaaggaaaa atcaaacgga tgtgtttcat
    16681 cattacgttg tttcagctgt ttgtcattat ttttgcagtt gattggtatt tattagtggg
    16741 aaagattgga gcttggtatc tgattccttt tctattgtgt ctgttgcttt ttgtatccca
    16801 tagttttttt agtacgctaa ttgatatctg caaagaagat aaagcagata gtcttgttta
    16861 tttgatcaaa aggaatatct ggctgttgtc aaaaaacgtc aaggggtttc tgtggtattt
    16921 atgccttttg tatggactgt tctggtgtat acagcatgat tttttactca ctgcattact
    16981 gtatccaggg ctgccatttg tcacagtatt ttgcttaaaa gaaaaggaga atgactttaa
    17041 atgaagaaaa aatatgtaca agtcggactt ggtgggagag cgcgtttttt ttatgaggca
    17101 attgcctcta cgtatcatca aacttgtgaa ctagtcggtt tttgtgatgt atctcaaaca
    17161 aggatggatt atgcaaatcg gatacttgtg aacaagtatg gacagaaaaa agtaaagaca
    17221 tgtatgccat cacaatttga gcaactactg gatgacgtac ggccggctga agtcattatt
    17281 acgtcagtag atcgaacgca tcatgattat atcattcgtg caatgaaaaa aggatatgat
    17341 gttatttgcg aaaaacctct gactatcgat gaggaaaaag ctcaagaaat aataacggtc
    17401 caaaaacaga cgggaagaag cctgcgagtc acttttaatt atcgttatgc accgtatcac
    17461 acgaagataa aggaactgat agaaagtgat gtgattggtg atgtgttttc tatccacttc
    17521 gaatggctgt taaatacgca acacggggca gattatttca gaagatggca tcgaaataag
    17581 cacaatagcg gcgggcttct tgttcataaa tctacccatc attttgatct agtgaatttt
    17641 tggttgaaca gccgtcccca aaaggtattt gcattcggtg gattaaactt ttatgggatg
    17701 cataatgcgg agaaacgcgg agtagaaata ttttacagtc gatgctatgg aagcgaacta
    17761 gctaaagctg atccatttgc tatagacctg tcagccaatg aggaattgcg agaaatgtat
    17821 ttagaagcag aaaaagacag tggatatatt cgtgaccgca gtgtattttc cgatgatatc
    17881 tctattgaag atacactagg cgtaacggtc aaatatgaaa ctggtgcgat tttgacgtat
    17941 tcgctaaacg cgtatatgcc gtgggaagga ttcaatgttt ctttcaatgg cagcaaagga
    18001 cggatcgaaa tgaaagtgat cgaaaagtct tacatcaatg caggtggaga taaaaaggat
    18061 gaaggagcag ccgttcttca gtccatccat gtctatccga tgttcggtga gccatatgag
    18121 gtaatcgtcg aacaagaagc gggcggacat ggcggaggag atccgcgttt attagatgat
    18181 ctatttggtg aagaaaaaga agaagatccg tggaatcggg cagcaactca cgtagatggt
    18241 attttgtcga ttttgacagg aatcgcagca aatcattcta ttgcttcagg aaaagcagta
    18301 gtgattgacg agcttgtcag cttaaaagaa taagaataga tttacggaaa ttacttcccg
    18361 tagtttataa aatcttgcct tattgccgat aggctgtctt ttgaacacta tttattcgga
    18421 aaaaggggct atgacatgct ttttgtcatg ccttcttttt atgtgggctg cttttttaga
    18481 taggagaaaa taaaaataac tatttaaacc cgaacaaaaa ggaataatgc attaaaaatc
    18541 attagatttt acaaaaaaat aagtaaacca cgttgtcagt ttcagaaagt agtgttagac
    18601 tgttttcaat atttgaaaca acatttttta ttcaaatatg gcaaactgcc ttcacgtgca
    18661 aaacagttgc aagaaaatga atttacacta ttattagttt tacataccta cttatatatc
    18721 gtcataatat ggatgacagt ttctagccag taccgtaaat gctggactat aagtaaaaga
    18781 ttggctattt cacccttctt ttcttatgat aaagtggagg gtgtttttct ttaacagaaa
    18841 catagtaggt ttttgcacaa aagcggttat ttcggtatta ataaatgata aaggagcaaa
    18901 taaagtgaaa gaattagttg aacgtattca aaaagacgga cgtatattag gcgagggcgt
    18961 attaaaagtc gacagcttcg tcactcacca agtagaccct gtattgatgg aacaaatggg
    19021 aaaacggttt gcagaagtat ttaaagaaca aggaatcacg aaagttgtga ccattgaagc
    19081 ttctgggatc gcaccagctt tatttgctgc aagagaatta gatgttccaa tgatcttcgc
    19141 tagaaaagca aaaagtttga cgatggatga agaattattg acagcttctg tttattcttt
    19201 tacgaaacaa gtgacaagta cgatttctat ttctcgtaaa tttttatctt cagaagacaa
    19261 agtattgatt atcgacgatt tcttggcaaa cggacaagcg gcaaaaggat tgatcgaatt
    19321 atgccagcaa gctggagcgc aagtagaagg aatcgggatc gtcatcgaaa agtctttcca
    19381 agatggacgt cagttacttg aagaaatggg attgagagtc gtttctcttg cgcgtattgc
    19441 ttccttgacg aatggacaag ttgagtttct tgaggaggac gcataatcgt ggaaaaagaa
    19501 acacaaaatg gaaaagctgc ggttttaggg ctgcagcatc tgctagcgat gtatgcagga
    19561 gcagtagcgg tgcctctttt gatcggtaca ggactaggct ttaatcaaga acagatgact
    19621 tatttgattt caattgatat ctttatgtgt ggattagcga cattattgca attgacagtt
    19681 aaccgatttt tcggaattgg cttgcctgtc gttttaggat gtgcgatcca agcagtagct
    19741 ccactgatct tgatcggaac caatgaagga gtaggagcta tctacggatc tattatcgct
    19801 tctgggatct ttgttgtgtt agtttctggt gtcttttcta aaattaaaaa actattccct
    19861 cctatagtaa caggaacggt tatcaccgtt attggtttga ctttgattcc tgtagcaatc
    19921 gagaaaatgg gtggaggtaa tgcaacagca gctgattttg gtgacaaaac aaatctgcta
    19981 cttgcatttg tgacgattct cctaattgta ggcgtgcaaa tgctggcaaa agggttcatt
    20041 cgttcaatcg cggtattgat tggtttagtc ggaggaagca tcttagctgc tttcatggga
    20101 atgattgatc tgagcgcgat tggagatgcg ccgttgttcc atgttccaca acccttttat
    20161 tttggtaaac caactttcga cgtatggtcg attttactaa tgatcatcat ttccatggtc
    20221 agcatggtag aatcaacagg tgtatatttt gcacttggtg acattactgg gaaaaatgtt
    20281 ggagaagaag aattgaaaaa gggatatcgt gcagaaggac ttgccgttat cttaggcggt
    20341 attttcaata cattccctta tactggcttt tctcaaaatg tcggattggt tcaactttcg
    20401 ggaatcaaaa caagaaaacc catctatttc tctgcattct tcttgattgt tttaggatta
    20461 ttccctaaaa tcggtgcgtt agcacaaatc atccctgagc cggtacttgg tggaggaatg
    20521 ttagtcatgt tcgggatggt ggcagtccaa ggaatccgta tgctgttgga agttgatttc
    20581 agcaatgata aaaatctatt gattgttgct gtttctgttg gatttgggct gggtttcaat
    20641 gttacgccga cattattcag tcaaatgccg caaacattgc aaatgttcac tggaaatggc
    20701 atcgtaatga gtagcattac ggctatcata ttgaatttga tctttaatgg attgaaaaaa
    20761 gatgaagaaa tattggaaga agccttcaag aagtaataga gcaaaagaaa ggttgtgaaa
    20821 tcagcgtttt gacctgattg ttagagaaac aaacggaaaa atagttcctc gtattttgcc
    20881 gtttgtttga gctaataccg agggtcagct gattgaacac cgttgaagga agaggtcgtg
    20941 agcctgccgt tcatctccga gtcacaagga acaatttcaa aaaaaagctt ctcatgtttg
    21001 ttgaatattg ttccttgtga ccgaggagtt ggcaggagaa caccgtttat ccataaaaag
    21061 gaatgtgacc taacttttgt cacattcctt ttttttatct ttaataaaca cgaacaataa
    21121 aatgtttttc tttgttaaca ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa
    21181 tatgaatgta gaaaaactgg gaggaaaaac aatgacagca aaaatctctg tgattatggg
    21241 gagtacttca gattgggaaa caatgaaaga agcatgtcaa atattggatg aattcggtgt
    21301 gtcttatgaa aaaaaggtcg tttctgctca tcgaacccca gatttgatgt ttgaatatgc
    21361 acaaaacgct cgaaaaaaag gaatcaaaat cattattgca ggagcagggg gagccgcgca
    21421 tctgccgggt atggtcgcat caaaaacaac tttgccggtt atcggtgtac cggtccaatc
    21481 acgaacatta aatggtttgg attcgcttct ttcgattgtc caaatgcctg gaggagtacc
    21541 tgtagctact acagcaatcg gaaaagcggg agcaattaat gctggactac ttgcgataca
    21601 gatgctttcc atgtatgatt tagaattgga ggctaaattg gctgaaagac gttcaatgtt
    21661 ggctaaaact gttatagaaa gtagtgatca acttggttaa acctttgttg cctggttcaa
    21721 cgatcgggat cgtcggtggc ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa
    21781 tgggctttaa agtaggtgtg ctggatcctg tgagcgattg tccagccgca caagttgcag
    21841 attggcatat tgaaagtgtc tatgatgaca cctttgcgct gaaagagctt gccagaagaa
    21901 cagatgtcat tacctatgaa ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac
    21961 ccatgtcatt tattcctcaa gggacagact tgcttgcgat tacgcaagac cgcctgttag
    22021 aaaaatcttt tttggaaacg aataacatcg tgattgctcc ttatgcaacg atcgtcagtc
    22081 cgacggatat ccaagatgcg attgacggga tcggctatcc ttgtgttttg aaaaccactc
    22141 gaggcggcta tgatggcaag gggcagtatg ttttgaaaag tcgggcggat ctagctcctg
    22201 ccatggacct tttaagagaa ggaacctgtg ttttagaagc atggatcccg tttgaaaaag
    22261 agatttcgat catggtggca ggaaatggtc aaggagattt cacgactttt ccggtcgtgg
    22321 aaaacatcca ccacaacaat attcttcatg aaacgattgc tccagccgcg attgatcagg
    22381 atgtgatcga ggaggctgag cgtattgccc gtgtgattgc agaagcagtg agcctttcag
    22441 gtgttttagg ggtagaaatg tttttaacaa aaacaggcgg gctatatgtg aatgaattag
    22501 ctcctcgtcc tcacaattct ggacattatt ctattgaagc ttgttctatg agtcagttcg
    22561 atgcccatat tcgaggtatt tgtggttggc cattaggaga aaaagtacgt ttgataagca
    22621 atgcagtaat ggtcaatgtt ttgggagaac aactgctgga aagctatcgc ttgattgaga
    22681 ataagccaga ctggcaattc catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga
    22741 tgggacatat cactatcttg acagaagaca tccagcaaac gctggaagaa atcaaacaaa
    22801 cgaatatttg ggattagagg agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg
    22861 ggaatatctg gacggaagag aacaaatacc gtgcgtggct cgaagtcgag attttagcag
    22921 atgaagcttg ggcagaactt ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc
    22981 atgcatcatt tgatatcgat cggatcttgg aaatcgaaaa attaacacgc catgatgtcg
    23041 ttgcgtttac acgagcggtt tctgaaacat tgggagaaga aagaaaatgg gtccattatg
    23101 gtctaacaag tacggatgtc gtagatactg cttatggcta tctcatcaag caagcaaatg
    23161 atattttacg ggaagacttg aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac
    23221 acaaatatac agtgatgatg ggtcggacac acggtgtcca tgctgaacca actacttttg
    23281 gattgaaact tgccacatgg tattcagaaa tgaaacggaa catcgaacga tttgaacatg
    23341 cggcaaaagg agtagaagca ggaaaaatct caggagcagt gggaacgttc gcaaatatcc
    23401 cgccatttat tgaagagtat gtatgtaaac aattggggat ccgtccacaa gaaatttcta
    23461 ctcaagttct ccctcgggat ctacatgctg aatatttctc agcgatggcg cttattgcta
    23521 caagtattga acgttttgca acagagatac gcggattgca aaaatccgaa acacgtgaag
    23581 tagaagaatt ttttgcgaaa ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc
    23641 cgatcggttc agaaaatatg gctggtctag ccagagtggt gcgcggacac atggtcactg
    23701 cctatgaaaa tgtcacatta tggcatgaaa gagatatttc tcattcttca gcagaacgaa
    23761 tcatcttgcc tgataccacg attttattga attacatgtt aaatcgtttt ggcaatatcg
    23821 ttaaaaatct aacggtcttt cctgaaaaca tgaagcggaa tatgtctgca acatttggtt
    23881 tgatttatag ccagcgcgtt atgctgaaat tgatcgataa gggaatgaca agagaagaag
    23941 cttatgatct tgttcagcca aaaactgcgt acgcttggga caaccaagtg cagttccgcc
    24001 cgctgttaga agaagatcca accatcacat cggtattatc taaagaagaa atagacgatg
    24061 cctttgatta tcatcatcat ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg
    24121 aataaagaag agtgagttgc aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa
    24181 gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt atcaataata
    24241 ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac aaaaaataga
    24301 ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg agatgttgca
    24361 gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc tggatacgat
    24421 aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt
    24481 ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt gctcggcgga
    24541 tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct ttgatcgaca
    24601 gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa aaaagctgac
    24661 cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattaac caaattttta
    24721 gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg gtataagatt
    24781 ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt acatattttt
    24841 gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg
    24901 tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta
    24961 tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata
    25021 tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga
    25081 attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa
    25141 tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc
    25201 tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc
    25261 cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga
    25321 tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc cggaagaaat
    25381 cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa
    25441 ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt
    25501 gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca
    25561 tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt
    25621 atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa
    25681 gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca
    25741 gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata
    25801 caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg
    25861 cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa
    25921 catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc
    25981 agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga
    26041 aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg
    26101 tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa
    26161 accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg
    26221 tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa
    26281 ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc
    26341 tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa
    26401 tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat
    26461 tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga
    26521 cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa
    26581 ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag
    26641 aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt
    26701 aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg
    26761 ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt
    26821 gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt
    26881 caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg
    26941 agttggcgga atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga
    27001 ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt
    27061 tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc
    27121 atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga
    27181 acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg
    27241 tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca
    27301 agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct
    27361 gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga
    27421 tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg
    27481 acttatattg acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat
    27541 gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa
    27601 aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga
    27661 cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc
    27721 attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga
    27781 atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga
    27841 tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca
    27901 agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg
    27961 gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc
    28021 aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg
    28081 taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt
    28141 ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc
    28201 tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac
    28261 accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt
    28321 caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag
    28381 tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc
    28441 cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag
    28501 tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca
    28561 tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca
    28621 atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg
    28681 agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac
    28741 tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt
    28801 atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat
    28861 ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa
    28921 ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg
    28981 ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc
    29041 ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta
    29101 ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt
    29161 tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag
    29221 atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt
    29281 gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt
    29341 gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca
    29401 tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat
    29461 gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg
    29521 aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag accagattca
    29581 aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa
    29641 gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca
    29701 agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac
    29761 attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa
    29821 ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt
    29881 gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa
    29941 gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt
    30001 caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc
    30061 ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa
    30121 aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg
    30181 ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa
    30241 aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt
    30301 agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga
    30361 acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt
    30421 tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt
    30481 ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa
    30541 tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa
    30601 aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg
    30661 aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga
    30721 ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga
    30781 aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg
    30841 gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact
    30901 gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc
    30961 ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg
    31021 agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact
    31081 tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga
    31141 gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag tcgaagaatc
    31201 gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg
    31261 gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc
    31321 gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa
    31381 aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg
    31441 aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca
    31501 aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc
    31561 gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga
    31621 atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat
    31681 gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta
    31741 gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat
    31801 tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa
    31861 attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa
    31921 ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag
    31981 gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga
    32041 cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg
    32101 gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc
    32161 aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag
    32221 atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta
    32281 gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag
    32341 tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg
    32401 caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt
    32461 gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc
    32521 gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat
    32581 attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag
    32641 atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga
    32701 aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag
    32761 cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag
    32821 atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt
    32881 atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag
    32941 actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc
    33001 ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac
    33061 gtaagccgac tccagcagaa gcaaaagcat tagtgtttgc ttggagagct gtcaaacatg
    33121 tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc
    33181 agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag
    33241 gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca
    33301 aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg
    33361 atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt
    33421 agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa
    33481 taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc
    33541 cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt
    33601 taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt
    33661 tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa
    33721 aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc
    33781 aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca
    33841 aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag
    33901 tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc
    33961 gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga gaagaattct
    34021 cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata
    34081 agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag
    34141 taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg
    34201 ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg
    34261 ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc
    34321 aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga
    34381 atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag
    34441 ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag
    34501 agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag
    34561 gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt
    34621 acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa
    34681 aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac
    34741 aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac
    34801 ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct
    34861 gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca
    34921 gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt
    34981 tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa
    35041 cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt
    35101 tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat
    35161 gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa
    35221 gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa
    35281 ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg
    35341 caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat
    35401 gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg
    35461 atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa
    35521 aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca
    35581 actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt
    35641 acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg
    35701 gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa
    35761 gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc
    35821 gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta
    35881 ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag aaatcccttg
    35941 ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt caaaagcaat
    36001 atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga ttctttgttc
    36061 tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga
    36121 gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat cggtaaaatt
    36181 ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc ttgaaggctg
    36241 tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt aaagcgagtc
    36301 gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt cttgcgggat
    36361 tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata aacgatataa
    36421 tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact tagatacttc
    36481 gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag aatcaactgg
    36541 aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa gggaactggc
    36601 ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga ttctttctgc
    36661 gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt catggaccaa
    36721 cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat caactattaa
    36781 aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct ccctaaataa
    36841 aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga cttggtttgc
    36901 tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt cagcaagttt
    36961 caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat gatttttttt
    37021 caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt
    37081 tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa gcagataatc
    37141 atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa
    37201 tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca acccgtccat
    37261 gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa ttgaagacag
    37321 tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag aaccaaggac
    37381 agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga taaaaacttt
    37441 atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt gtgacagttg
    37501 ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg taataaagaa
    37561 ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa tcgatccctt
    37621 tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc ccgttgtcta
    37681 taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata aatcctttgt
    37741 gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc aagatcattt
    37801 ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca agcatatggc
    37861 cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct ttttcaagag
    37921 gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca
    37981 tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca tccaaactgt
    38041 tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt tgtgctaaat
    38101 ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc attttattca
    38161 tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga aagccaaggt
    38221 tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct tggacctttc
    38281 tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga actccaagtt
    38341 ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg cttgctaagt
    38401 cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg acagaatcta
    38461 cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata ttattgatca
    38521 gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct aacgtactaa
    38581 tttctttaag tgtatttgta ttatttttgc tctctcgaat atcattccta agttcttcta
    38641 ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag tcccacatac
    38701 tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg atatttgcga
    38761 tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt agcaaaggga
    38821 taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt atgcttaaat
    38881 tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc
    38941 tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata
    39001 tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg
    39061 actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata
    39121 gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca
    39181 ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt
    39241 tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg
    39301 tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta
    39361 cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa
    39421 ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca
    39481 aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc
    39541 tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt
    39601 gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc
    39661 actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca
    39721 tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc
    39781 tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt
    39841 gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa
    39901 aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc
    39961 agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa
    40021 aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca
    40081 atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa
    40141 tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat
    40201 taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta
    40261 tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag
    40321 aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag
    40381 cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg
    40441 gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct
    40501 atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg
    40561 gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag
    40621 gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt
    40681 acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat
    40741 ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag
    40801 caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt
    40861 cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga
    40921 gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat
    40981 cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa
    41041 atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg
    41101 aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg
    41161 ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga
    41221 aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat
    41281 tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc
    41341 gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa
    41401 cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta
    41461 tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga
    41521 agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa
    41581 agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac
    41641 agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct
    41701 agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa
    41761 atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc
    41821 gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc
    41881 acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca
    41941 gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc gcggaatggt
    42001 gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt
    42061 taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca
    42121 catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg
    42181 cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg
    42241 tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg
    42301 actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg
    42361 ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga
    42421 aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat
    42481 ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg
    42541 gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg
    42601 ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg
    42661 tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa
    42721 cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa
    42781 attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa
    42841 aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg
    42901 tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc
    42961 gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag
    43021 ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt
    43081 ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag
    43141 ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt
    43201 atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga
    43261 cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt
    43321 ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt
    43381 taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga
    43441 aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag
    43501 cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt
    43561 tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag
    43621 gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa
    43681 caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat
    43741 tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg
    43801 atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca
    43861 tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag
    43921 gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc
    43981 tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa
    44041 gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc
    44101 gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc
    44161 tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa agccttgatt
    44221 aaattaataa tgttcgtgtt ttgctggatg tgaacatcag gaggataatg taatgaaagt
    44281 atttgattac gaagatattc aattaattcc taataaatgt atcgtaaata gccgttctga
    44341 atgtgataca acggtcacat taggtaaaca tacattcaaa atgccagtag tgcctgccaa
    44401 catgcagacg attattgatg aaacaatcgc agaattctta gcggaaaatg gttatttcta
    44461 tatcatgcat cggtttgatg aagcagctcg aattccgttt atcaaaaaaa tgaaaaagcg
    44521 tggattgatt acctcgatca gtgttggcgt caagaaagag gaatattcct ttatagagaa
    44581 gttggcagaa gaatcgctaa atcctgatta tatcactatc gacatcgcgc atggacatgc
    44641 taactcagtt attgatatga ttcagcatat taaaaaatat ttgcctgaaa cttttgtgat
    44701 tgccggtaat gttggtacac cggaagccgt ccgtgaattg gaaaatgcgg gcgcagatgc
    44761 aacaaaagtc gggattggtc caggaaaagt atgtattaca aaaatcaaga caggctttgg
    44821 gactggaggc tggcaactgg cagctcttag atggtgtgcg aaagcagctc gcaagccgat
    44881 catcgctgac ggtgggatca gaacacatgg cgatattgca aaatctgtcc gttttggtgc
    44941 gacaatggtc atgattggtt cattgtttgc cggtcacgag gaatctcctg gcgaaacaaa
    45001 agtcgaaaat ggaatcgtgt ataaagaata ctttggttct gcttcagaat tccaaaaagg
    45061 cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa cacaaaggat ctttgaaaga
    45121 tacactagtg gaaatgcaac aagaccttca atcatctatt tcttatgctg gaggaagaga
    45181 tctggaagcc attcgaaaag ttgactatgt catcgtgaaa aattcgattt ttaatggaga
    45241 cacaatctaa aaaataaata aaatattagg atgttgtgaa aaagttgtcc tgcattaagt
    45301 aataagaaca gccaaacaaa aatagttctt tatatgtttt gtttggctgg aattattatc
    45361 gcagatacaa gcttttgaac atcattattc agaataaggg actgtgataa gatttttgtc
    45421 acagtccctt atgttttgca aaaaaagttt ttcttttttc tcgtaaattc ggtaaaatca
    45481 aaagaaaaaa tagacaatag aaaagagaag cttatgtggg aaaaagtaga gaaaaaacaa
    45541 ggaccgattt atcgtcaaat tatggaacag attatgagag aaatcgaaaa cggaaggatc
    45601 aatcctggaa gtcaacttga atcagaacga aaactagggc aactttttgg tgtgaatcgt
    45661 tccaccgttg tccatgcatt ggaagagctt agggagcttg gggtactaac gagcaaacgt
    45721 ggcagcggat ggtttgttaa tcagactgaa tgggggaaat tcgctgttcc aagaatcgat
    45781 tggcggcaaa tgatctctcc tcgttatgag cagacggact ggtatgagca aaaaataaga
    45841 gaagcaaagc agatccaaaa aacatcgttt cttgatttat acgctagcga gatgccagat
    45901 gagcttttgc cgaattttga gtttccttct tattctcttg aagaaatctt agcggaagaa
    45961 aaagaaatgg atcttttagg ctatactccg ttacgtaaca aaatcaaacg gtatttggaa
    46021 agtaaatttg aattggcatt ttcccccaac cagcttcttg tgactggtgg cggtcagcaa
    46081 gcaatctttt tgattttaca aacgattctt tcagctggag aggggatagc tgtagagtca
    46141 ccttcttttt tttaccgatt acctcttttc aaagcttcag gaacgcgttt atttgggata
    46201 ccgatggatg aagaagggat cgacttagaa aaactgaaag aatcgatcca taaaaataaa
    46261 ttgaaagcag tgcttatcaa cccgaatttt caaaacccta caggaaaagt gatgagtcaa
    46321 aaacgacgag aaaagctagt agaattgtgt cgacagtatc ggcttccaat tattgaagac
    46381 gatgtattta cggatctttc atttctaaac caagaagaag tccttcctat acgaacgatt
    46441 gatccggata atgtgttata cgtcggttcg ttatctaggg ttttaggaaa aacaacgaaa
    46501 atcggttggg tgataggacc tgagactttt atctcgcaac tagctcaagc gcaagaaatg
    46561 atggagtttt cgatgaatat ccttacacaa atcacagtgg ctaatgtatt taatgaagca
    46621 attgatttga aaatgcaaga tgtaagaaaa aagttgaaag aaaaaagccg actgataagc
    46681 gcttggggag attcacaatc gtttttcaag cttcattcaa ttttaggtgg ttactatgcc
    46741 tggattactt gggatgggaa aaaactagac cccgaactag cagaaaaagt agtagatatg
    46801 ggtcttggaa ttgcaccaag tctacttttt gggcaagata taaatggaat cagaatcaat
    46861 tacagtaggc tggatgaaaa ccaaataccg ttgttttatt caaagatgaa aattctagaa
    46921 aagtggttaa cagaataatt tcacaaagta aatagattaa agaatataac agcttggatt
    46981 taaatttcaa gctgtttctt ttttttcgga aaaagaatac gatataaaga aactaagaaa
    47041 aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt gcaacaaaac
    47101 tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt agaaaataga
    47161 aaaaatgcag atgaaatata aaacgctttc cttattgtga ttttattcac taatgctgtt
    47221 aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta gcttgtattt
    47281 ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca gttttattca
    47341 ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg tgtgacaaat
    47401 ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca atgcagattt
    47461 cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt tagtacctga
    47521 tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc gtgtattaga
    47581 ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac caactgctgg
    47641 acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg ttcttcttcc
    47701 aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc aagctttttt
    47761 atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag ctctaccacc
    47821 acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag gtttgaagaa
    47881 acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat cacaaggtga
    47941 tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat tttatatcca
    48001 aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa aaacacttgc
    48061 acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg atccgcttgc
    48121 agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg gaccagtcct
    48181 aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg atccaacccg
    48241 ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga tccgcttccg
    48301 taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag tcatcgaagc
    48361 aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa aacaaaaggt
    48421 ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag aacaaattgc
    48481 aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat ccaagcaatc
    48541 acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt tggtgaagat
    48601 gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa gttcggagaa
    48661 gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag tttcggtctt
    48721 gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat tttcgaagca
    48781 atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg cactagaaat
    48841 ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga attgcattcg
    48901 gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat tccttccaac
    48961 ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc ggttgttttc
    49021 ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga agcttacgaa
    49081 gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat tatcacatac
    49141 ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga aaatatatct
    49201 gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat cattcaatcc
    49261 gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc gggcgttgcc
    49321 gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc cccaatcgga
    49381 cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt ctggttgcca
    49441 aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga aaagaaggga
    49501 cgaaacaaag tcatggctta tcaatttaaa ttgccggata tcggtgaagg aatcgcagaa
    49561 ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga tgatacatta
    49621 ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt cacctgttac aggaacggtt
    49681 aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg gggatgtatt agtagaaatc
    49741 gatgcaccag gacacgaaga caatgaaggg gacagtggtg tagccgcaga atcccaaact
    49801 ccagctaaac cggctgctga acccactgtt gatacagaat cagctggatc gtcaagcgaa
    49861 ggagtcttcc aatttaagtt accggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc
    49921 aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa
    49981 aatgacaaat cagtagaaga aattccgtca cctgtcacag ggacagttaa aaacgtgatt
    50041 gttccagaag gcactgtagc aaatgttggg gatgtattag tagaaatcga tgctcctgga
    50101 cacaatagtg caccgtcaac tagtgcgcca tcagctgaag caccaaaaga aaaagtggaa
    50161 acatcaggtt cagcaagtgt cgttgaagct gcagatccaa ataaacgtgt attggctatg
    50221 ccatctgttc gtcaatttgc ccgtgaaaaa gatgtagata tctcacaagt aactgcaact
    50281 ggaaaaggtg gcagagtaac aaaagaggac atcgaaaact tcttggcagg tggtccaagc
    50341 agtgcaccag cgaaatcaga agcgccagaa gcggcagcac cgaaagaagc agctcctgca
    50401 gcagaaagca aaccagctgc accagcaaaa ccattcaagt ccaacttagg agacttggaa
    50461 gaacgtgtag caatgacgcc aacacgtaaa gctatcgcta aagcaatggt caacagtaag
    50521 catacagctc cacacgtgac attgcatgat gaagtagaag tgtctaaact atgggataat
    50581 cggaaacgat ttaaagaagt tgctgctgca aatggtacga aacttacttt cttgccatat
    50641 gtcgtgaaag ctttaacagc gactgttaag aaatacccag tattgaatgc atctatcgac
    50701 gatgcaaacc aagaaatcgt ttataaacat tactacaata tcggtattgc aactgatacg
    50761 gatcacggac tatatgtacc aaatgtgaaa gatgcagacc gcaaaggaat gtttgctatt
    50821 gcagacgaaa tcaatgagaa agcaaaatta gctcatgacg gcaaactatc tgcagaagat
    50881 atgcgcaatg gaacaattac gatcagcaat atcggatctg taggtggcgg ttggtttaca
    50941 ccagtcatca actatccaga agtagctatt ttaggtgtag gaacgattgc acaacaacca
    51001 atcgtaaatg ctgaaggcga aatcgttgtc ggtcgtgtga tgaaactgtc attaagtttc
    51061 gatcaccgta tcgtagatgg agcaactgcg caacaagcaa tgaataatat caaacgtctt
    51121 ttagctgatc cagagctatt aatgatggaa ggatgataaa caaatggtag ttggagattt
    51181 tgctgtagaa ttagatacag ttgtaattgg tgcgggacct ggtggctatg tagctgccat
    51241 ccgcgctgct gaaatgggac aaaaagtagc aatcattgaa agagagtata ttggcggtgt
    51301 ttgtttgaat gtcggctgta ttccttcaaa agcactgata gctgcaggac accattatca
    51361 agagtcacta gattctgaaa tgtttggagt aactagtgaa aacgtcaaac tagattttgc
    51421 aaaaacacaa gaatggaaag aaaacaaagt tgtccatacg ttgacttctg gtgttggttt
    51481 cctattaaaa aaacataagg ttgaaacaat cgaaggagaa gcgttcttcg tagatgatca
    51541 cacactacgc gtcattcatc cagattcagc tcaaacttat tcgttcaata acgcaatcat
    51601 cgcaacaggt tcccgtccaa tcgagattcc aggatttaaa ttcggaggac gtgtgttaga
    51661 ttctactggc ggattagcat tgaaagaagt acctaagaaa tttgtgatca tcggtggcgg
    51721 tgtgattggt gccgaattgg gcggagcgta cgcaaactta ggagcagaag taacgattct
    51781 tgaaggctca ccacaaattt taccaactta cgaaaaagat ctagtaaaat tagtagaaga
    51841 tgatttcaag aaaaaaggcg taacagtcgt aacgaatgca atggcgaaag aagcagtcga
    51901 taacggtgac agcgtgacag tgaaatatgc agtggacggg aaagaagaat ccgttactgc
    51961 tgattatgtg atggtcactg taggtcgtcg tccgaataca gatgatatgg gcttagaaca
    52021 agcgggtgtc gaagtaggag aacgaggatt gatcacggtg gataaacaag gacgtacgaa
    52081 cgtacctaac atctatgcca tcggtgatat cgttccaggg gcggcattag cacacaaagc
    52141 cagttatgaa gcaaaaatcg ctgctgaagc aatctctggc aaaaaagtag ctgtagatta
    52201 taaagcaatg ccggcagtag ccttcactga tcctgaattg gcttctgtag gtatgacgat
    52261 caaagaagca aaagatgctg gtatcgaagc aaaagcatat aaattcccat tttcagggaa
    52321 cggtcgggca ttatcgcttg gtaaaacaga aggattcatc cgtctagtta cgacgatcga
    52381 agataacgtg cttattggcg cgcaaatcgg tggagtcggc gcaagtgata tggtctctga
    52441 attagcttta gcaatcgaat caggaatgaa tgctgaagat atcgccttga cgattcatcc
    52501 tcatccatct cttggcgaaa tcgtcatgga tgcttcggaa ttagcattag gtttaccaat
    52561 ccatatttga tttgaataat acaggcgaaa cgcaactgaa
//
LOCUS       DAZUMV010000005        49278 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000005 DAZUMV010000000
VERSION     DAZUMV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.005"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..940
                     /locus_tag="ACG8FK_000289"
     CDS             <1..940
                     /locus_tag="ACG8FK_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5095930.1"
                     /translation="DFYQKERYWFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSY
                     RQPWIQKDKVMEFPSRELTDYHIGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGH
                     IHVPTVLNEKQTIVYPGAPQGHTKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKE
                     ISLRKARTTKEVIVHIRQQLSKVEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLL
                     EEFSDRINDFFIWRISLIENTFYTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSH
                     QEAARILSELPEYQEETLEKAKQLLNQDFLFEEDQE"
     gene            937..3657
                     /locus_tag="ACG8FK_000290"
     CDS             937..3657
                     /locus_tag="ACG8FK_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5095931.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3641..4585
                     /locus_tag="ACG8FK_000291"
     CDS             3641..4585
                     /locus_tag="ACG8FK_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5095932.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(4654..5664)
                     /locus_tag="ACG8FK_000292"
     CDS             complement(4654..5664)
                     /locus_tag="ACG8FK_000292"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5095933.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(5844..6188)
                     /locus_tag="ACG8FK_000293"
     CDS             complement(5844..6188)
                     /locus_tag="ACG8FK_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5095934.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6181..6615)
                     /locus_tag="ACG8FK_000294"
     CDS             complement(6181..6615)
                     /locus_tag="ACG8FK_000294"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS5095935.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            6753..7490
                     /locus_tag="ACG8FK_000295"
     CDS             6753..7490
                     /locus_tag="ACG8FK_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5095936.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7483..8694
                     /locus_tag="ACG8FK_000296"
     CDS             7483..8694
                     /locus_tag="ACG8FK_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5095937.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            8898..9680
                     /locus_tag="ACG8FK_000297"
     CDS             8898..9680
                     /locus_tag="ACG8FK_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS5095938.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            9750..10412
                     /gene="trmB"
                     /locus_tag="ACG8FK_000298"
     CDS             9750..10412
                     /gene="trmB"
                     /locus_tag="ACG8FK_000298"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS5095939.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10510..12264)
                     /gene="ade"
                     /locus_tag="ACG8FK_000299"
     CDS             complement(10510..12264)
                     /gene="ade"
                     /locus_tag="ACG8FK_000299"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS5095940.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12396..13325
                     /gene="comGA"
                     /locus_tag="ACG8FK_000300"
     CDS             12396..13325
                     /gene="comGA"
                     /locus_tag="ACG8FK_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS5095941.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13282..14331
                     /gene="comGB"
                     /locus_tag="ACG8FK_000301"
     CDS             13282..14331
                     /gene="comGB"
                     /locus_tag="ACG8FK_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS5095942.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14328..14606
                     /gene="comGC"
                     /locus_tag="ACG8FK_000302"
     CDS             14328..14606
                     /gene="comGC"
                     /locus_tag="ACG8FK_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS5095943.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            14581..15051
                     /gene="comGD"
                     /locus_tag="ACG8FK_000303"
     CDS             14581..15051
                     /gene="comGD"
                     /locus_tag="ACG8FK_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS5095944.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15023..15370
                     /locus_tag="ACG8FK_000304"
     CDS             15023..15370
                     /locus_tag="ACG8FK_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095945.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            15333..15758
                     /gene="comGF"
                     /locus_tag="ACG8FK_000305"
     CDS             15333..15758
                     /gene="comGF"
                     /locus_tag="ACG8FK_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS5095946.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            15730..16089
                     /gene="comGG"
                     /locus_tag="ACG8FK_000306"
     CDS             15730..16089
                     /gene="comGG"
                     /locus_tag="ACG8FK_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS5095947.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16261..17268
                     /locus_tag="ACG8FK_000307"
     CDS             16261..17268
                     /locus_tag="ACG8FK_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5095948.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17293..18477
                     /locus_tag="ACG8FK_000308"
     CDS             17293..18477
                     /locus_tag="ACG8FK_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS5095949.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(18914..20497)
                     /gene="abc-f"
                     /locus_tag="ACG8FK_000309"
     CDS             complement(18914..20497)
                     /gene="abc-f"
                     /locus_tag="ACG8FK_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS5095950.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKKVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(20602..20730)
                     /locus_tag="ACG8FK_000310"
     CDS             complement(20602..20730)
                     /locus_tag="ACG8FK_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095951.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(21075..21218)
                     /locus_tag="ACG8FK_000311"
     CDS             complement(21075..21218)
                     /locus_tag="ACG8FK_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS5095952.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21441..21920)
                     /locus_tag="ACG8FK_000312"
     CDS             complement(21441..21920)
                     /locus_tag="ACG8FK_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5095953.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(21993..22277)
                     /locus_tag="ACG8FK_000313"
     CDS             complement(21993..22277)
                     /locus_tag="ACG8FK_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5095954.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22355..22549)
                     /gene="ssrS"
                     /locus_tag="ACG8FK_000314"
     ncRNA           complement(22355..22549)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8FK_000314"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22621..23907)
                     /locus_tag="ACG8FK_000315"
     CDS             complement(22621..23907)
                     /locus_tag="ACG8FK_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS5095955.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24045..24716
                     /locus_tag="ACG8FK_000316"
     CDS             24045..24716
                     /locus_tag="ACG8FK_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS5095956.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            24682..25170
                     /locus_tag="ACG8FK_000317"
     CDS             24682..25170
                     /locus_tag="ACG8FK_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS5095957.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25183..26130
                     /gene="prmA"
                     /locus_tag="ACG8FK_000318"
     CDS             25183..26130
                     /gene="prmA"
                     /locus_tag="ACG8FK_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS5095958.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26131..26895
                     /locus_tag="ACG8FK_000319"
     CDS             26131..26895
                     /locus_tag="ACG8FK_000319"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS5095959.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27128..29341
                     /locus_tag="ACG8FK_000320"
     CDS             27128..29341
                     /locus_tag="ACG8FK_000320"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS5095960.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29352..29798
                     /gene="dtd"
                     /locus_tag="ACG8FK_000321"
     CDS             29352..29798
                     /gene="dtd"
                     /locus_tag="ACG8FK_000321"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS5095961.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            29969..31228
                     /locus_tag="ACG8FK_000322"
     CDS             29969..31228
                     /locus_tag="ACG8FK_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS5095962.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31317..32264)
                     /locus_tag="ACG8FK_000323"
     CDS             complement(31317..32264)
                     /locus_tag="ACG8FK_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5095963.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            32658..33950
                     /gene="hisS"
                     /locus_tag="ACG8FK_000324"
     CDS             32658..33950
                     /gene="hisS"
                     /locus_tag="ACG8FK_000324"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS5095964.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            33965..35734
                     /gene="aspS"
                     /locus_tag="ACG8FK_000325"
     CDS             33965..35734
                     /gene="aspS"
                     /locus_tag="ACG8FK_000325"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS5095965.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            35843..36718
                     /locus_tag="ACG8FK_000326"
     CDS             35843..36718
                     /locus_tag="ACG8FK_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5095966.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            36984..38867
                     /locus_tag="ACG8FK_000327"
     CDS             36984..38867
                     /locus_tag="ACG8FK_000327"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5095967.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(38942..39226)
                     /locus_tag="ACG8FK_000328"
     CDS             complement(38942..39226)
                     /locus_tag="ACG8FK_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5095968.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39216..39950)
                     /locus_tag="ACG8FK_000329"
     CDS             complement(39216..39950)
                     /locus_tag="ACG8FK_000329"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS5095969.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40079..40702
                     /locus_tag="ACG8FK_000330"
     CDS             40079..40702
                     /locus_tag="ACG8FK_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS5095970.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            40894..42015
                     /locus_tag="ACG8FK_000331"
     CDS             40894..42015
                     /locus_tag="ACG8FK_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5095971.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42022..45150
                     /locus_tag="ACG8FK_000332"
     CDS             42022..45150
                     /locus_tag="ACG8FK_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5095972.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45169..48363
                     /locus_tag="ACG8FK_000333"
     CDS             45169..48363
                     /locus_tag="ACG8FK_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS5095973.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48509..49216)
                     /locus_tag="ACG8FK_000334"
     CDS             complement(48509..49216)
                     /locus_tag="ACG8FK_000334"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5095974.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16027 a         8713 c        10325 g        14213 t
ORIGIN      
        1 tgatttctat caaaaagagc ggtattggtt ctcctttcct aaaaatgtcc atttatttac
       61 atccgaaacg gtagagacaa aaaaaataac gataaaaagt ggagaaactg tctctctcag
      121 tggatttagt tatcgtcaac cgtggattca aaaagacaaa gtgatggaat ttccttctag
      181 agaactcaca gattaccata ttgggttgta ccacggggaa cctggcgtct ctcaaaaagg
      241 aaactatgct cctttccaac cctcaaagat gcaagaaaag ggatacgatt attgggcttt
      301 agggcatatc catgtgccaa cggtcttaaa tgaaaagcaa acaattgttt atccaggtgc
      361 gcctcaaggt catactaaaa aggaacaagc ttctacatct attctactag ttgaattaag
      421 taaaggaagt tgccaaatac atccgattaa agtagcagag gtctattgga agacaaagga
      481 aatatcattg aggaaagcaa gaacgacaaa agaggtcatt gttcacattc gtcagcagct
      541 ttcaaaagta gaggatggat ttagtttaat tgagataaaa ttaaaagatt atgaccatct
      601 caatacagat gttttggaac ggatacaatc aggagagcta ttagactatt tgttagagga
      661 attctcagat aggataaatg atttctttat ttggcgtatt tctctcatag agaatacttt
      721 ttatacgaag accccacttg ctgcttctgc taaattgatg gaacagttat ttcaatacta
      781 tcagacacct caagatttcc aacaaatttt aaatgaagta tatagtcatc aagaagcggc
      841 tagaatattg agcgaactgc cggaatatca agaagaaaca cttgaaaaag ccaagcagct
      901 gcttaatcaa gattttttat ttgaggagga tcaggaatga agattttaag gatcgagatt
      961 gctgcttttg gaaaatggcg tcaaaaaagt tttgactttt attcaggcaa tcagctgatc
     1021 tatgggggaa acgaagcagg aaaatcaacg atttatcaat ttattcaagc aatattattt
     1081 ggtttccctt caaaaggccg taaaaaaaag gattatacac caaaagacgg ctcagcctat
     1141 ggcggcaaaa tatggcttaa acatcctgta tatggtgaat ttgctgtcga gcgctacaag
     1201 cagcaaaatc gcggaaaatc gaaggtgtgg ctgggggatc aagtgggaag tgatgagtta
     1261 ctagaaaaac tgatcgcacc tttgacgaaa gagttattcc agcaggtatt tactttccag
     1321 caagagcagt tgatggaact agatcatttg agagaaaaag agctacatga tgcgctgatt
     1381 tctttaggca tcacgggcag ttctcaagta ttccaaaagc ggcaagaata ttatcagaaa
     1441 gcacaacaat tattcaagaa aaaaggccaa aaattactaa tcaatcaaaa attaaatgaa
     1501 tggaaagagc tgcaagaaaa aattcatcaa aaacaagaac aggaagctta ttttggcaag
     1561 ttgatccaac aagaacagga atacgagtcg caacagcagc agttattggc acaattaaaa
     1621 gaggcgaaca aacaattttc attagccaga cagcaagaac ttaatttttc tttatatgaa
     1681 gaatggcaat ctctcaaaaa aaacagcact acaggtcttc ctgatgagca acttctttta
     1741 gatctggagt ctttctccaa acggtatcag caaacaaatg agcgtttgga agaattagaa
     1801 gcaacactgg aaaagcatag cgggatggat cagcagtcag ccagatatta tttttattta
     1861 gagcaggagc aggaactaaa agatctccag caacttcaat ttacagcgga acaattaaac
     1921 gaagaatggc aaagattaca aattgagact agagaaacaa attcagaatt gaagattttg
     1981 gaacaaaggt ggcattggca gaaaaatccg ttaccggaag aatttttata tgaagaagaa
     2041 tggaagcatc ttctggctga agaaagaagg attgctgatc tagaaagcca gatttctgtg
     2101 cgtctccaaa tagctaaaga acagcaagag atgattgaag cagaagtgaa tgacttggaa
     2161 gagacctatc cagcattatt acaaaatcag tcagagatag caccagtctc ttcatctaaa
     2221 ccgtatatgc tgatggctgg cggagcaata atcattgcag gtatatttgt accaatgccc
     2281 ttgaaagtga tccttttact aattggtgca ggcatgattg gctatttctt ctacaaaagg
     2341 caaccaaaaa ttaaaactaa aaatgatttc tctgagataa aaggaatgtg gcaagaaaaa
     2401 ctaggacaac ttgatctagc cttagctcaa ttagctgaag cagaagaaaa acaaaagaga
     2461 actggagaag aaaaacagat tcttcagcaa gaagtatatc gttttgcgga tcatcggcac
     2521 ttaggaaaga tggatacgct caaactcatg aaagaatatg gacaggaagt catagattat
     2581 cagaaattat atggcgtatt tgagcggaaa cataaaagag aacaggaaat acaagaaaaa
     2641 ctacgacaaa tcgaccaaaa atttgagttt gttaaagaat ggttaccgat acaagataag
     2701 acaatttcag aaaaaatggt attgctttcc cgttttatac aagagatgga acagatgaaa
     2761 tttgccagaa gctatcagca aaatacttta ttgaagcaag aaatcaatca attgaaaaag
     2821 aaacaaaaag tatggataca agaatacagc gaacaattga ctcaagcagg cttatcttat
     2881 ccttcggaag tacctgtctt tttacaaaaa gccaaacaag ccgaacagca gatgaaaaga
     2941 tacaatgaat tatcggaaat gcttactcca ctatttcctg aagagctgac aaaagagaaa
     3001 ttatctgtaa gactgcgcca actgaaagaa aagcaatccc aactccagca agaagaaaaa
     3061 gaagcaagtg aaaatcggca acgactccaa cttcaagtag aagagttgca aagagacggc
     3121 acattggacg aactttatca acaagctagc cggcaaaaag caaaattaca ggaattactg
     3181 gaggaatggg gagcacttga aatcgccggg acattcttag gtgatctttc aacagagatg
     3241 agtgaaagac aattacccca gttgttaaga gcagcaggtg attatttagg tattttaaca
     3301 aaaggaaatt atcagaagat attactgaaa aatgatacac tgactttaac agacggatat
     3361 acagaatttc ccatctatga gttatctaca ggtacgaaag atcagctgat tatggcgatg
     3421 agatttgctt acctctcatt agaagctaac cgttcgatat gcccgatcat cattgatgat
     3481 ggctggcttc attatgatca tcagcgaaag tatcaattag ctgaactttt agctaaattt
     3541 ggagaaaagt atcaaataat ttgtttttct tctgataaag aaatggtaag ctattaccat
     3601 gagatgaacc aaatcgtaaa acaattagaa ggagcaaata atgaaaaaaa ttcgtgattt
     3661 agtaatcgat gagcagtttg aacaatttgt cctgatcaaa agtgcagatg taagaatcgc
     3721 taaaaacgga aaaaaattta ttgcttttac tttccaagat acttcaggta ctatcgatgg
     3781 taaatactgg gatgcttcgg aggaggaaat caataaattt acagccggca cagtcgtttt
     3841 tctaaacggt aaaagagagg tttatcaagg aaaccctcaa gtaaagattc ttcatatgcg
     3901 cttaacaagg gtagatgagc caggagagcc tagtttatac atggaaagag cccctttgaa
     3961 gaaagaagaa atggaagaag aaatcaatca attgttgttt gagatcacca atgcgcattg
     4021 gaatcgtatt gttcgctatc tacttaataa atatcaaaaa caattttttg agtttccggc
     4081 agcaaaaaga aaccatcatg catttgccag tggattagct tatcatactt tgacaatgct
     4141 tcacttaggg aaatcaatcg ccgatgaata tcctgagctg aatcgagcat tgttgtatgc
     4201 tggtatcatt ttgcatgatt taggaaaggt gattgaactt tccggtccta tgtcgacaga
     4261 atatacgctt gcaggaaatt tgatcggtca tctggtattg attgacgaag aaataactaa
     4321 agcctgtctt gcgttgaaaa tcagcgaagc tgacgaagat gttctcgtat tacgtcatat
     4381 ggttttagca caccatggct tattagaata tggctcacct gtacgtccaa gaataatgga
     4441 agcagaaata ttacatcaaa tcgacaatat cgatgcttcg atgcagatga tgttgacttc
     4501 gattcgtcaa acagaaccag gaaaatacac cgatcggatt ttcggtatgg acaaccgaag
     4561 tttctatgta ccaaaagatt tatagcaaaa atttattttt ttcattcaaa acaaaaaaag
     4621 cgagacataa gtcttcgctt tttttgtttt cttttatttt gatgaatcag ttgtttcttc
     4681 agttgaagat tcttttgttg aatcagttga ttttgtgtca gaactctttg ttgaagctgt
     4741 agtttcactt gagtctttcg tacttgatga agttgttgtg aatgcactca acacattttc
     4801 aaaggcatca tctttgattt ttacatttgc atcttttaat tcttcaccga ttactttagt
     4861 tgtgaatgca ttgtcactta gtttagtttc agtagcgata tcttttagtt gatctttgta
     4921 tttatccatg tcattgcctt tattttggtt tttcaccatt ttgacaacgt agtattctgt
     4981 cgcataagaa gtaggatttg ttgtagtaat aacatcggag atctcaccgt ctttcaattt
     5041 gaaagctgct tcttttactt ctgcaggaat agttgttgtc gttgagtcga atttaacttt
     5101 accgccatct tcttttgtct ctgtatcagt tgatttgtct ttggctaatt tagagaaatc
     5161 atctccatca tccgctgatt ttttcacatc ttttgcttca tcttcacttg ataatttgat
     5221 gatttgtgct tctacttctg gatggaaaga tttccaagca gttttcaaat cgtcatctgt
     5281 aatatctacg tgagctttca agccagcttc aaaagctaaa ttgttacgaa tatattcttt
     5341 ataagtatct ttcgtatatc cagcagcttc taattgtgat tcaaaagtat cacctaatga
     5401 tttcgcttgt ttgtcgtatt ctgcatccac ttgcttatcg gttactttat cgccatattt
     5461 gttattgaat actttataaa tgatcatccg ttgtactaag gattggttcg atgattcaag
     5521 ttttgcttca tcatagaaat cagatacagt gatcgttccg cctttcatcg ttgcgatatc
     5581 tttattagta tcgcctgaac aagctgctag agttaaaaca gccaacgcac tagtagctgc
     5641 caaaataatt gatttttttt tcattaaaat ttgcactcct gtaattagtt attattttta
     5701 tatattaact ataccatatc ccatttcctt taaaaacgtt gtcgtggtag tttcaaacaa
     5761 aattcaaaat ttcatcacat tcctaggaaa tacgcaaact tttctcactt atatagttaa
     5821 atctgttgag aaaataatca ttattatatt tgttcaggat actctggcag atcagctttt
     5881 agtttttcta cttgttcatt gatttgtgtg atacgtggtt cggcttggaa tttaaaatct
     5941 tcgatatctt ttttgattgc ttcttggaaa gcaggaatca cagattcagc tgtttctttt
     6001 gtttcgatca atgcgctttt aaaatgattc aggctgttat ttaaatcatt tgtcagagtt
     6061 gttgcttctt ccaattcttc gatcagtttc tttcttgttt cattcccgct cctgggcgcc
     6121 aataacagac ctccgacagt gccagcagca gctccaaata acagtccttt gaaaaagttt
     6181 ttaatcatcg caggacacct gctttacgat tgtttcttga atagctttta gttcttccga
     6241 tccataacta tcttgatgat tgacaaagtg aatagaaaaa tcatcctctt tgctgtagcg
     6301 agggactaaa tggatatgtg aatgaaaaac agattgatag gctagttcac ggttgttatt
     6361 caagatatta agacctttca tttctggaaa agctttttcc aatgcttgag caatcttagg
     6421 cacacgcgaa aaaacctctc ctgcaagttc gggatcatat tcaaaaatat ctgctacatg
     6481 acgtttaggc acaactaatg tgtgtccttt cgttgcttga gagatgtcta aaaaagcata
     6541 gaccttgtcg tcttcatata ttttatagct tgggatttct tgattgatga ttttgcaaaa
     6601 aatacagttt tccatataaa acatcctttc tataaagaat aaatacttca gcttaacttt
     6661 accataagtt aaagctattg taaggaaaaa aacaaaatac cgtcattttc ttcgtggtat
     6721 aataaataaa aataaatggc ggaggaaaaa gaatgagttt aattatcgat catgtcacag
     6781 gggggtacgg acatctccct gtgttaaaag atatcagttt cgatgtacag cctggagaga
     6841 tagtagggtt gattggactg aacggagcag ggaaaagtac gaccatcaag cacattatcg
     6901 gtctgctgga agcttcaaaa ggaacgatag aaatcaatca tctatcctta aagaaaaata
     6961 cggaagaata tcgcaaacaa attgggtata ttccagagac tccttcttta tatgaagaat
     7021 tgacattgag agaacatatc gaagtcactg cgatggccta cgatatacct atggatatag
     7081 caatgaaaag agcagaaaag ttgttggaga cttttcggtt atctaatcgt cttgactggt
     7141 tcccagctaa tttttcaaag ggaatgaaac agaaagtgat gatcttatgc gcctttttga
     7201 tcgagccttc tttatatatc atcgacgaac catttttagg cttggatcca ctagctatca
     7261 atgcattgct tgaattgatg gtagaaatga aaaaagaagg agccggtatt ttgatgtcta
     7321 cgcatatttt agctactgca gaaaaatatt gtgatcgttt tgtggtctta cataacggag
     7381 agatacgagc agacggttct cttgagatgt tgagaaaaga atttcagctg ccggattctt
     7441 ctttggacga aatctatatt gctttaacaa aggagcaggc acatgagtag tttttttcaa
     7501 aaacgtttag caagacatca aaagaaaatg atgaaatata tgcgttatgt attgaacgat
     7561 cattttgttt tagtttgtct tttcttgatt ggcggtgtgg gattatatta ttctaattgg
     7621 ttgaaaaccc tgcagccacc gtttgcaatt ggagggctca taatctctgt attttggatg
     7681 ctctgtttat tcgtgggtaa gatggctact tttgcagagg cagcagatat tgttttttta
     7741 cttcctaaag aaaaagaaat gcaaacctat ttggaacgag cttttcgcta ttcttgcatt
     7801 tttccgttga tctttttgat attggcatgc ggattggcca tgccattagt ggtggttagt
     7861 acggagcaat ccttttcgat gtttttcgtc tatctgatca tattatggag tttgaaattc
     7921 agtcatctgc aaatcaagag agcagaattg tttcgactag atcaacaagt tattcgttat
     7981 tggaaattag gctggttttt ttcttctctg atctttttgt ttctatcatt ttattggcta
     8041 atatgggcag gaatagcagg tgcattagta caggcaagtc tatattactg gtttttgtgg
     8101 aagcaacaag aaaaaggatt ggattgggag aaaatggtgc atgtagaaga acataggttg
     8161 catcagatct atcaatttat caatttattc acagatgttc ctgaaattac ggcaaagatc
     8221 aaaagaagac gctatttgga tcctatattg actcatatta aaagagaatc aaaaaacact
     8281 tatctttact tgtttgcaag aagattggca agaggttctg attttagtgg tctttatttc
     8341 cgattagtaa ttataggtgg gttattgatt gccggagtag tcgactttta ttttagtctg
     8401 attatcggtt tcttattttt atatttgatc ggtttccaac tgttaccttt agcgaaccag
     8461 ttccgctata tggttctcgt tcagctttat ccaatccata agaatcagaa gaagcaggcg
     8521 attcagtatc tactcacatg ggtattaatt atcgcgtcgg ttatttttgg tttgattgct
     8581 gcagtcgtat tagatgggca gcagcgatgg gttccgctag cggcttattt gattgttact
     8641 atgctctttg ttaaactata tgtaccaact cgtttgaaga agatgtcaga ctgaaaacaa
     8701 agctgtgtag ggtaaaaatg agaatgaaaa atagagcgtt gacagtaaga atgctgttcg
     8761 ttctattttt tttgagaaaa gctggttttt tgaatggcaa ttaaatgaaa aagaacgtat
     8821 atgtgcttga cgtgtagtgt tcaggcgact agaataataa gtactgcaaa acaaagagta
     8881 agaggacggg agaatgcatg atggagtttc agttagacca agagtggcgg ttacgtccca
     8941 tcaaaggcga cacaggaaaa acatacatag gcatgcgtga tgaggacaaa gtctttatta
     9001 aacgaaacac gactccgatg ttggctgctc tttcaaggga aggaattaca ccaaaacttg
     9061 tttggacaaa acgaacgggg aatggcgata ctttgacagc acaagagtgg ctggaaggtc
     9121 gcttgctgac tcctaatgag attggtcaaa gaaacgacgt gattgatgtt ttgtaccatt
     9181 tacatcattc aaattctctt aaaaccatgc tgacccgtat cggtggtcgt gtcatgacgc
     9241 cggagaagat gctgcaaaaa tatgaaaaag aattaccgga agaactccag aaaaatcaat
     9301 ttctgcaaat tgtccatcgt tttcttcgtg agcagatccc gtcaatagat aaggaaaact
     9361 tatcagtagt acacggagat gtaaaccatc gtaattggtt agtatgtaaa aattatttat
     9421 atctagtcga ttgggattca atcatgtttg cagaccctgc ggtggatatt gcaacgattt
     9481 taggaaatta tgtgccactg tctagctgga atcagtggct gatcagttat ggcatacgcc
     9541 cgactgacga aatgcttgaa aaatttcatt ggtatgcggc catgaatttg ctccaagaaa
     9601 tcaccagata ttgcaaacgt gaagatcatc gacggatgaa cgaagaaatt ttacaattaa
     9661 aacgaatttt cagtggctga ggtttggccc aaactctgcg gagatttggc cagacctttt
     9721 taattagagg aaaggaatgg agataaataa tgcgtgtccg aaatcgtccg ggagcagcgg
     9781 aaatgttagc ggctcacccg aattttgtaa ttagtgatcc aacattgtgg aagggaaaat
     9841 ggaatgaatt gtttgaaaac gatcatccaa tccacattga gattggtatg gggaaaggcc
     9901 agttcatcac tggaatggca aaagcccatc ctgaaatcaa ttatattgga gtagaaatgc
     9961 aggtcagtgt tgtttcgatt gcactagaca agttgatcga acaaccgctt cctaacctga
    10021 aacttctaca tgtggatgga tcagcgttga cagagtattt cgctgacagc gaagtagatc
    10081 aaatctatct gaatttttcc gatccatggc caaaaaaacg tcatgaaaaa cggcgattga
    10141 catataagac ttttcttgct gtagatgaac agattcttcg tccaaatgga gaaatccatt
    10201 tcaaaacaga taatcaagga ttgtttgagt attctctggc aagtttttct caatatggca
    10261 tgatcctcaa acaggtatgg ttagatttac atcaaagtca atttgaaggc aatattatga
    10321 cggagtatga agaaaaattt tcttcaaaag gtcaacgtat ttatcgagta gaagctcaat
    10381 ttcaagataa gaccaaaaag aaaagtctgt gaaactaact ttctgctttc ataaaaacat
    10441 taatatccat aaataaatcc cagaaaatga acattacaga tgtaatgtat tttctgggat
    10501 ttatttgatt taaaatgtat ttatttcatc ggtttttcca caaggtggaa tcaattggaa
    10561 gcgagaaaat gaaaataatc ctttgtcggt gatcttcaat tcgggtatga cagataatgt
    10621 taagaaagaa agtgtcaaaa aaggatcaaa ggttttagct gctcctagtt gatgtgcttt
    10681 agttattaat tcattcaact gttcatagac gatcgaaaaa ggttcctgtg tcatgattcc
    10741 gcctatagga agagaaacac aagctatttc ctgctgtttg ttgacaacgg tcatcccgcc
    10801 acctttttga atcaaaagat tagcagcata cagcatatcc tcgtcgtttg tcccaacgac
    10861 aactagatta tgcgagtcat gggcaatcgt tgtcgctatc gctccttgtt tcagctgaaa
    10921 tcctttcact atgcctaatc caatgtttcc cgtttgatgg tgacgctcga tcaccgcaat
    10981 ctttaattgg tctttttcga ttgaaggaat gaatcgttcg ccttgtcggt ccactttttc
    11041 taccaaatct ttagtcacta gactattcgg gataatttcg atcacatggg cttgtgaaga
    11101 agaaagaagc agctctaacg aatgtggatt taatggttga gcgtttagtt ttggcagaca
    11161 acacgtatat gaatttgaat gatcagcaaa taaatggtct tggagttgcc cagattgaac
    11221 aactaatgtt cccttataga aaacttcatg aattggcagc ttggtaagat cgtctgttaa
    11281 gaagaaatca gcttgatacc caggggcaat cgcacctagt gttttcaaat caaaacattc
    11341 tgctgcattc aatgttgcca tctgaatggc ttgtaatgga tcgattccaa gtgcaatagc
    11401 ttgacggacg atatgatcga ttgatccttc ttttactaaa tcattgatca gtttatcatc
    11461 ggtgacaaag aagcatcttc ttgaattttc aggtgtgatc gccggtagta gcgatgccag
    11521 atcttttgca acggtccctt ctcgaaccat taaatacata ccaagatcca aacgttcttt
    11581 agcttctttt gcagttgtag cttcatgatc cgtccgaata cctgctgcag gataaacatt
    11641 taactcttcc atgccgatac cagctgcatg cccgtcaatt tttttcttat gctggtgcat
    11701 caattggatc ttttctagga tgtctgtttc attatttgct actgcttgat agttcatgac
    11761 ttcagctaag cctaaaactt cgggtttttg taaaaaaggg gctaattttt ttgcatttaa
    11821 atttgcacca ttggtctcaa aaggggttac aggtacacaa gaaggtagca taaaaaagat
    11881 attcatgagt gtttgtcttg catcttctat cataaactca agacctgtac tcccagcaat
    11941 gtttcctatt tcatggggat ctgtcactac agtagtaact ccatgaagca aggatacttt
    12001 tgaaaattcg cttggtgata aaagtgagct ttcgatatgc acatggccat cgatgaatcc
    12061 tggcactaga tattgcccat tagcattatg tacgcttttt ccttcgtatt caccgattcc
    12121 tacgatcatt ccttcgcaga ttgctacatc tttttccatg atttctttcg taaagacgtt
    12181 gacgatctta gcatgcttga tcactaggtc agcaggcttt tttttacttg ctacttcgat
    12241 atatttcctt aatgttgttt ccaatggagt gcctcccaag ataaattttt ataagtataa
    12301 cataaattcg gtttttgcca atattttttt gtaaattcat tttatttgaa acaaaaagat
    12361 caaaaagacg tatgtttttt gtggagggga gaataatgga aataaaacga ttatcggatc
    12421 aaatgataag tgaaagtttt gccaaacatg cccaagacgt atatatattg ccagcttcag
    12481 aagtatttta catctatttt cgctatggaa aaaaacgaac attctggaag aaagtctctt
    12541 cggcagatgg acagcaactg atctcaagat ttaagtattt gggacagatg gatatcggag
    12601 aaaaaagaaa agctcagtta ggtgcattta cttattcttt agaaaaacag gaagttcgtc
    12661 tgcggttgtc ttctgtagga gattaccttc aacgagagag tttagtgatt cggctattat
    12721 acggtacttt tgaaacaccc catcgttgct ttagttctcg agattttgca actattttac
    12781 agaaaaccgt cagacgtgga ttataccttt tcagcggacc agtcggatca ggcaagactt
    12841 ctctaatgta tcggttggct ttggaagaaa aattgcaagt gattactatc gaagatcctg
    12901 tagagataga agaaccgttt tttctgcagc tacagatcaa tccgaaaata gcacagactt
    12961 atgatacact tttgagactc gcattacggc atcgtccaga cttgctaatc attggcgaga
    13021 taagagatga atcaactgca catgctgcgg taagagcagc actaacaggg caccgggttt
    13081 ttgcaaccgt acatgcacgc aatcttgaag gtacattggt cagaatccat gaactgacaa
    13141 agaaaaaaga agagctatct gaatgtttag ctggtgtcat ttatcaagaa cttttggaag
    13201 atgccaatca ccatccttct gttttatgga gttatcaatt tatggaggaa gaatcagtaa
    13261 aaaaatcatg ggaggaaagt tatgaagaag ctcaggaaaa tatttggcag tcagaactat
    13321 tctaaaggat taacaagaag ccagcaaaac cagtttgcac aaaccatggg agaactgctt
    13381 ttatctggtt ttagtctgca agaatccctt tatttattac tcacgatccg ttcgtttcca
    13441 cctgttattt tggcaaatat ccaacatcag ctaaaggaag ggcaagcgtt tagtgaggta
    13501 ctttcgcaaa taggatatgg agcagagcaa ctggtacaga tcgagttagc tgaacgtcat
    13561 gggaatctgc cgacaacttt gttggaaatt gccaaacagt ttcggcttgt tgaaggattt
    13621 cgcaatgatt tgaaaaagct gcttgcatat ccgatgctat tgctcacctt tttgctaggt
    13681 atcttgttta gtatgcggtt gttcgttttg cctcagctta ttggttcagg aatggtcatg
    13741 caagagcatt ggggtattca gctgatacaa gtctttcatt ggtatgttct actattcatc
    13801 ctagcggtta ctctattggc tttactagta aggtatcgtc tgatgaagtt gtctcctgtt
    13861 gatcagagtg aatggataag cagacaatta ttgattggac cgatatattc cttgtatcaa
    13921 tcggcttatt tgtcactaga gattggaaag ctttttcatg aaggtttcga attaaggcaa
    13981 atcatagtct gcttaaagga aacgaaacaa gaaagtctga ttcagcatct tgcctctaag
    14041 ttgattcaag gattggaggc aggtatgtca ttggctgagc agttccagaa ttatcgtttt
    14101 ctatcagaag aattcagtaa aattgtttta cagggagagg caaagggaag tttaggtaaa
    14161 gaactgattt tttacagcga atggacaaga aaacagcttt tcaaacgtct tcatcactgg
    14221 atgcatttta ttcagccaat catttttttg ggcgtagcgg ctttgatttt attagtttat
    14281 gccgctgttt tattacctgt ttacggaagc atagaggagg ttttgccatg aaaaagagaa
    14341 aaatatctta ttatagcggt tttacattga tcgaaatgct aatcgtctta ctgatcatca
    14401 gtgtattagt gttgttgttc gtccctaatt tgtcgcgtta taggaatcat gtagaccaag
    14461 aaagtcgtga agcgatcatc cagttggtag acacacaaaa agagctctat gcgcttcaaa
    14521 ataatggaag agttcctact gtggaggaat tgttgaatga aggctatatc aaacgagaac
    14581 atgcagaaat ctatcagcgg ccttgaaggt aaggttttag catcaggata tacgttgttt
    14641 gaatcactct tactgatttt attacttaca acgatccttt cttttccagt tctcgcattt
    14701 tctgcatgga agaatgaatt ggcagtctat caatttttcg gccaatttga aaaacgaatc
    14761 tattctacgc aaaaaatcgc tattgtgaat cagatccaaa caggctttta ttggaatagt
    14821 gaagagaatc aaatcatttt tcatgttccg aatccagata aaataaattg ggaagtatta
    14881 gatattcctg atgagattac cttgaaacgc catgcttcta ttacttttgc tgctagaacc
    14941 gggaatgaaa gttcactaaa agcttatcaa ttttattggg aagaaaaaga gcggacgatt
    15001 acgtatcaat tccagatagg gagtggtcgc tatacaaaaa gaattgaata aaacaggatt
    15061 tgttttgttg gagtccctta cagcgcttat gatcgcttct gtctgtgtct acttagtgag
    15121 ctttggccag ctgtttttgc ttcaggcaga aaagaaaagt ggggaggaac tgctatttat
    15181 tcgaatgatt tatgaagata tcaagacgca gcggatgtat cacaaaagac cagagcgaag
    15241 ggaagaaatg atcccatacg atatccaaat tgtcgataat aatggagtaa tcgaaaaagt
    15301 gtttgtcaat atggagaaag aagcgatcag tattgaaaaa acaagtaatt ccagcattta
    15361 cgctgattga atgtctaatt ggactgatga tattgagcag tctattgctc acattcaatc
    15421 ttttgattca gcaaaccatc caagtaagtg aatttttgca gcgtaaagat caaaaagagt
    15481 ggctaatatt tttagcacaa ttagaagaag aactcaagag cagtcacaat gtctctgtga
    15541 aaaatcagca actgcactac acgatcgagg ataaacagta tatcatcgaa agatatcagg
    15601 caatgatacg taaaagaaga acttccggcg gacatcaacc tatgttaaca agtatcactg
    15661 agcttcaatt attggaaaaa gaacacacaa tttcttttta tgtacacttt gaaaatggag
    15721 aagatggata tgccatatgg acaaaaaacg atcagtagaa aatgttataa gggctctatt
    15781 ttattttctg tcttgctttt atttctttta tgtacgtttt tattgatggc agcattagaa
    15841 agctaccgtt tgtcaatgga tcttaatata cgaatcaaaa attattatat agcaaaaata
    15901 atgaagcaga tggctttatc ggaacagtct acgcttgccg aaagttcgga aggagttttt
    15961 tactacacaa ctggatctgt aacatatcag tgggtacaac agtccctatt cttagaggtg
    16021 gaagtctcac cgtttatctt tcggttcaca gaagaagtaa aaaatgatac agatactagt
    16081 acagaatgat ggaatgttcg tgaaaacgcc ctcttttctg ttataacact gtttttaata
    16141 aaaaaatgtt tgccgtttca caaataaaaa acagtgaaaa cgattgaaat tttgtcaatt
    16201 gttcgataga attaaacagg tgaaaaaatt gttataatat atctagaaat gaggtgcacc
    16261 atgttaccag aaaaaatgga acaagggttt aatcaaaacc tggaagctat tcagctttta
    16321 cagaatgcat tgggaacttc ctttttagaa gcctatgtcg aaaatgcaga aaatttaata
    16381 gatgattatc aagtacgtgt ggtggatgga gtgccgacaa aagagacaac gcaacggatc
    16441 acagctcttt atgaagagtt gaagaaactt tctttcgagc cagaagaatg gcgcagactg
    16501 tctcaattac ttttattgaa gggcagccag acagaacatt tgcaggcgaa ccatcaactc
    16561 actcctgata gtatcggttt tttatttgtt tttcttattg aacaactata cacaaataaa
    16621 aaagcaccgg ttaagattct ggatattgcg gcaggaatgg gcaacttgtt attaacagtt
    16681 cttttaaacc tatcgaatgc ggggtaccaa acagaaggaa ttggtgtgga tattgatgat
    16741 acattgttgg ctgtcgcagc ttctacaagt gatttgacac aagcaaatgt tcaatacttt
    16801 catcaagacg ggttgcagga attattaata gatcctgtcg attttgcaat cagcgatttg
    16861 ccaatcggtt attatccgaa tgacgaaaaa gcaaaagagt ttttaacaag taccgaagaa
    16921 ggccatagtt atgcacatca tttattgcta gagcaatcaa tgaaatatgt gaagccggat
    16981 ggttttggtt tgtttttgat gccgagtgga tttttagaaa cggatcaaag tgaagaaatc
    17041 aaaaaatggt ttaaagaaga aggttatctt caaggaatga ttcaattgcc agatgaattg
    17101 ttccgtaata aacaatcgca aaaaagtatt ttgatcttac agaaaaaagg accacaagca
    17161 caacaagtaa aagaagtctt gcttgtgaaa cttgcttcat taaaagaacc agagaaggtg
    17221 acggaatttt tcaacgagtt taaaaactgg aaatcatctg ctctgtaatc attcaacgag
    17281 aaggagagtc atatgtctaa aacaattgca atcaatgctg ggagttcaag tttgaaatgg
    17341 caattatatc aaatgccaga agaaaccgta gttgcgaaag ggatcgtcga acgtatcggt
    17401 ttacaagatt ctatttttac catcaagtac ggcgataatc aaaaattcga acaaatcatg
    17461 gatatcgaaa accacgaagt agcagtaaaa atgctgttag atcagttgat tgaactgcaa
    17521 attttaagct cttatgatga aatcaccgga gtagggcacc gtgttgttca cggaggagaa
    17581 acttatgggg attcagtagt gatcgatgag aaagtaatgg accgcattgc tgaattagca
    17641 gaatttgctc ctttgcataa tcctgcaaac ttaatgggga tcaaagcgtt taaaaagatt
    17701 ttacctgaca ttttgagtgt ggctgtattc gatacatctt tccactcaac aatgccaaag
    17761 caaaattatt tatatagctt accggtgtct tattatgaag atttcaaagt aagaaaatat
    17821 ggcttccatg gcacaagtca tcgttatgtg tcggaacgtg cggcaaaatt attgaacaaa
    17881 ccgatcgaag aactaaaaat catcacttgc cacttaggaa atggtgtatc gatcacagca
    17941 gtcgatggag gtaaatccat ggatacttct atgggattca caccattagc tggggtgaca
    18001 atgggtacac gttcaggtga tatcgatcca gctgttctcc catatttaat ggaaaaatta
    18061 gagattgata ttgatgaaat gatcaatgtt ttgaacaaga aatctggtct gctaggccta
    18121 acaggattgt ctagtgatat gcgtgatctt gaaaagaact atgaaaaaga acatattcgt
    18181 ttagcttatg acattttcgt ggaccgcatc cgaaaataca tcggcggtta tgtgacaacg
    18241 atgaatggtg ttgatgcaat tgtctttact gctggtatcg gcgagaatga cggacatgta
    18301 cgtagtgaaa tcatcaaagg tatgtcttgg tttggttgtg aaatcgatcc agcgttgaat
    18361 gaattgcgtg gtgaagaagt agatatctct acaaaagaat caaaagtaag agtattagtg
    18421 atcccaacag atgaggaact aatgattgcc cgtgatgtgg agcgtctgcg cggctaagcc
    18481 aaagcattcg ttaggataaa agatgaaacg catgcaagag ggaattcttg catgtgtttt
    18541 ttttgcggag aagaccgcaa aatggtaatt taacttcaat ttcccttctt ttttacagga
    18601 tcttcttagg gaattttgct tggaaaagtg gtcaaaaggg aaaagactgg cattttattt
    18661 attgattttc aggaaaatcc caatatttta ttttcattca tctttataag ggacattaca
    18721 tatgtaaaaa gaaaaaacat ttttagatca gcctcgtggg ctgtcgactt tattcttcgc
    18781 tttatgttta gctagaaaac cgatccgaaa cattcattga tcgaataata aaaacttgat
    18841 tttccacctg tactttgcct gaatcgtgaa agcacaggta tgaaaatcaa gttttttgtt
    18901 gcgaaaaaac gtcctagtca atcagttggc cgagcggctg gcggctgatc tgctcctgat
    18961 caataacgag tgcttcttgg aagtgttgct gcaaaaagta gcggtcatgg ctgactgcta
    19021 ggagcgttcc ttgatagtct tgcagcattt cttcaatttc ttctcgagca tagctgtcga
    19081 ggtgattggt tggttcatcc aaaatcagga cgttgatttt ttctaaaaag agtttcagca
    19141 acgccaaacg gattttttca ccaccggata aatctttgat ccgtttaccc acatcgcttt
    19201 gataaaagcc acttttggct aattgccgtc gagcgacttc ttcgtttcca gtcaatgtga
    19261 tggcgtaagc cagcaaacgg ctatcgggat cggcaaactg cagctgctgc ggcaaataac
    19321 cgatttgcaa gctactacct aatttgatgg tgccaccgta aggcttttcc tcaccgagta
    19381 ataatttgag taaggttgat tttcctgcac cattgtcgcc gatgatggct aaatgatctc
    19441 cacgatagac tgcaaaactg ctgtctttga atagtacttg atcagcgtac tgctggtgca
    19501 agttttgaat gatgaaaact tttttgccag attttccacc attagacaag ctttgcagcc
    19561 gatttttcgg gggaggtggc ggtttgacga gtgtcagttt cgccaggcgg cgttccagtt
    19621 ctttggcttt tttgaagaag gactcattgt ctccttcatg tgcccactga cgaaaacgac
    19681 gaatcatgac tttcaaccgc tggacttctt tttgctgcag ttcgtaattt tttgtcagct
    19741 cagccaagcg agcttctttc aagactcgaa aacggctgta gtttccggga tactcaatca
    19801 atgcaccttc ttccagttcc catgtttttg tggtgacttg atcgagaaat tgccgatcat
    19861 gagaaacgac cagaaccgat ttttttgtat tctgtaaata gttttccaac cagcgaatac
    19921 tgttaagatc taaatgattg gtcggttcat cgagtaaaag taaatcggca tcggctgcta
    19981 aaattttggc taaagcgact ttaactcgtt cgcctccgct aagttctgtt aaccgtgctt
    20041 cctgacggtg tggaatccct aagccttgca agatcgctgc aatccggtct tctaatacgt
    20101 aaccgcctag ttcttcaaag cgatgctgca gctcaccgta acgggccaga cacttctcta
    20161 agtttgtatc tggttctgtc atcaatgctt cgtagcgact catttgctgt ttgaggtcct
    20221 gtatctcagc ggcagaatgg agcaagtagt ggttgacggt cgcttcggaa actgtaagtt
    20281 cttgttcgac cgtcccgatc tttaagcctt ttttgcgact gataacaccg ctgtcgacac
    20341 cttctctgcc ggtcaagatt tgtaaaaagg ttgatttgcc agttccgttg ataccgacta
    20401 aggcaatttt ttcaccagct tcaatttgac aggataaatc ctcaaataat ggattcccgc
    20461 cgattgtttt tttaatagaa attgcttgta tgatcatgat ctctctcctt gagaccactc
    20521 aaaaaggcca tggttatcca tggcctgtgt tatttaaact cagattatca tgagaaaaat
    20581 accttgagat ggccattgat tttacgtgaa ttcaactgtt ttggacaaac ttgtcccgtc
    20641 aacagcaact gtaaaaccaa ttgcatggct aaatgcgata tggtatcgtt ggtaattttc
    20701 attacttgtt attttagtca tgcgcatcat agtattttcc ctccttcgtg tagttgtctt
    20761 ttccagtgta cctgaaagtg tcagaaagtt caactagcgc tgacatttac ttaatattta
    20821 tcgatgtgta attctgtcag agagcttttt ttatctgact gagggtaatg tctcgtggac
    20881 cacatcattg tattctgagc taaaccagcg ttcaggcgaa atcatatggt gtttgataaa
    20941 aaactttgtc tacatgactt ccttttgcca tttgcttaat accgttgcat taaacagctc
    21001 ttctctttgg aaatctcacc aacatcgcgt tcaggagtat cgttgtggtt gcaatggcaa
    21061 gcggtagcct ttcatcaatt cttgaatgat ttaccgaatg tagcacttgc cttctcgcgg
    21121 tacccgaacc cgaaataaaa ttttagtttg gcgataaaat cggtatccgc catcgatact
    21181 tcatattttt tcgtatcaga ttcatcggaa acttccacat tgacgaacca ataagctttg
    21241 gcatgcttag gtcgttttaa accacaccag ggcgactaat cgccctggtg ttcacttttt
    21301 tttattttac ttgaactcat tgactgtttt ccctttattt taaacattat aaagaccgat
    21361 gagtgcttgc cgcgtctttt gtttttatca cggttttttg tgcaaaaaaa cctgccgata
    21421 gttggcaggt cgtctacgtg ttaagctttg gtgtggtctt tagctggcaa ttggttttct
    21481 aaatcagttc ggacaacaag cacatcgcaa gctgcgtttc taataacata ttcagaaaca
    21541 gaaccaataa ataaacgttc tacagcattc aatcctgtgg ctcctaacat aatcaaatct
    21601 acttgattat cttctgggat ttctctggca ataatttgtt ttggtgagcc atattctacg
    21661 acagaggtta cattattcaa gccagctttc ttcgcattgc tttcataatc tgccaatgtt
    21721 tgctttgcca tttcagtagc ttgttctgca agcattccat caaaggatga aacggtttgg
    21781 aaagaacgtg tgtcaatgac atgtgctaat agaagttctc cgttatttcg gatagctaca
    21841 ttgaccgcct ttttaaaagc taattcagct tcatcagaac catctacggc aaccatgatt
    21901 tttttgtatt gttgtaacat aagcattcac tcctcaagtc aatcgtattg tactctttta
    21961 ttgtagaaca ctttagggaa aaagagaatt atttactgct ttggttcagc aatttttttt
    22021 gcaaatcgga tactgaaggt ggcagaaaca ataattacga tcagtaaaaa gaccaaggca
    22081 ataaagcgtt gattgaaaaa agctgttaaa atacccatta cgcctaaaac agcatatgca
    22141 gctccgtaaa attgtaggaa tccttgattt ttatcatttt tgttgatcaa tacaaaaaag
    22201 atatcctgtt tattaaataa ataatagcta acgaataata atactaaagt ggcgataatc
    22261 attaaaatcc taatcatgaa caaatcctcc aaaataagca acaaatagaa aaagcaattt
    22321 gccaagagca aattgcttta ttctttaaaa gatggtaatc tccgttaatg ccgttgtttg
    22381 tcctgtagta ttgatcccgc gaattcaagc aggtgggtcc catggtcgta gcttcgaact
    22441 accaaatgat aaggcatgtt accagcagtg acacaagacg ggtcccaaga tatcaataaa
    22501 aatgttcggt caacacacaa agaggacaat tcgaacatca cagatttccc acctctatag
    22561 tagcatatga atttaaaaag agcaatgaga gagctcgcag aaacagaacc gttttcttgt
    22621 ctagtttttt ggaggatgct ttttccactc ttttatacgg taatactgtt gacccagagc
    22681 ctgttcatat ttccctgtat cttttggctg ataatactga gcattcttca gtttatctgg
    22741 taaatattgc tgattgaccc aagcctgatc aaaatgatga ggatactgat aacccacgcc
    22801 acggttgagt gatttcgcac ctttgtaatg gctatctctc aaatgatcag gaacatctcc
    22861 agctttgccc tctcgaatat cagcaagtgc tgcatctaat gccatgtagg cagaattgga
    22921 ttttggcgac aagcaaagat cgacgacgac gtctgctagt gggatacgtg cctcaggcaa
    22981 tcccaatttt tcagcagcta atacagcgtt cactgtacga gccgcagcag caggattacc
    23041 taaacctata tcttcataac cgataaccat cagacgccgg caaatacttg ccagatcacc
    23101 tgcctcaact aaacgggcga gataatgaag tgcagcatcc acatcacttc cacgaatcga
    23161 tttttgaaaa gccgaaataa cgtcataatg agcatctcca tttttgtcat gggtaagagc
    23221 ttttctttgg acacattctt caatgatcga cagtgtaaga tgaatccttc cttctttatc
    23281 tgaaggagtc gatagtgtag ccagttccag cccattcaat gcactccgca aatcgccgtt
    23341 cgttgcacgc gaaaggtgaa gaagagcatc ttcgtctaac tggattgctt gttgtccaag
    23401 gccgcgttct ttatccctta aagcatgttc gactgccaac tggatgtctt gttctgttaa
    23461 tggtttgact tcaaagatct gtgtacggct gcggatcgct ggattgatgg taatataagg
    23521 attctcagta gtcgctccga tcaagatgat tcggccactt tctaaatgag gaagcagaaa
    23581 atcctgtttt gttttatcta atcggtgtac ttcatccaat aagaggatca ctgtaccact
    23641 catttttgct tcttccgcta caacttgtag atcctttttt gaatctgtag cggcgtttag
    23701 catgcgaaat gcataattag tagagccggc gatcgcactt gcgatactcg tttttccagt
    23761 accaggaggt ccatatagaa tcatggaaga gagcatgcga gcatcgacca tgcggcgtat
    23821 gatttttcct tccccgacta gatgctgctg accaacgact tcatcgatcg ttcgaggtct
    23881 catgcgataa gccaatggct gttgcatagg attttcctcc ttcttgtttt tacgtccctt
    23941 aattatatca tatttagcaa gatgcctaga aagaacagcc ttctgttgtt ttgttttcat
    24001 agcttatcca actaatattg tgtatgataa agtaggtgag aaaaatgacg gataaacaaa
    24061 agatagatga atttttttgg aataaaacga cgccagaagt ggcacgggat ttattgggga
    24121 tgtatttgga gcatgacaca ccagaaggaa gattagctgg atatattgta gacgcggaag
    24181 catacctagg tccagaagac gaggccgccc atagctatgg tttaagaaga acaccgcgtg
    24241 tgcgggcaat gtatgaaaaa ccaggtacga tctaccttta cacgatgcat actcatcgga
    24301 ttttgaacat cattactcag ccagaaggga ttcctcaagg agtaatgata cgtgcaatcg
    24361 aacccgccgc aatgattgat caaatgagta aaaatcgtgg cggaaaaact ggtccagata
    24421 tcagcaacgg tccgggaaaa ttagtggaag cattagcaat tcctcaagaa ctttatggtc
    24481 agtctatagc agatagttcg ttacgcttag tttttgagaa aaaaaagaca ccaaaaaaga
    24541 tcaatgcttt gcctagaatc ggtatcccaa acaaaggtgt atggacagaa aagccgttgc
    24601 gttttgttgt gtcgggaaat ccttatatct ctttacaaag aaaaaaccaa atcgagaaag
    24661 attggggttg gaggaaagaa aatgaaaaag aaggatctat taacatattt agatgaaata
    24721 attgaaaaac aagtgacaga ctatgatgta gctttagatt gggatacaaa aaatcatacg
    24781 atcgagatcg ttgttcgctt gtttgcagaa aataaagcac aatttgagat tgatgatgca
    24841 gaagggatcg tttcgcaaga accaattatc gaatttgaag acggcgtact tctttttaat
    24901 cctcagaaat ctgtttttga tgaacgcaat tatctagcag taatccctta tgaaggaaaa
    24961 aaagggttgg caaaatcagt agctgattct ttagtggaat atctaaatga agtattagct
    25021 caagggcaaa gcgatttgct tgattttttg gatgaagacg atgacgaagc tgtattcgag
    25081 cttcactggg ataatcagca actagcagaa ttagtagaag taaaacagca aaatgaagca
    25141 gatacttatt taccgtaccc aagttattaa ggagtgacag atatgaaatg gaatgaagta
    25201 aaagtagaaa ctgaaagtga agcagtcgaa gcagttgcta atattttgat ggaggcaggg
    25261 gcgagtggtg tcgcgattga agattcttta gatgttgaac attttcagtc ggatcaatat
    25321 ggtgagattt tagataaaaa tgattttaca tcgctgaaag aaggcgcact agtcatggct
    25381 tattttccgg aaacgacttt cttaccggaa atcttgcctt ttatcaaaga tcgtatcaca
    25441 caattacctg agtttggtct agctattgga aaaaataaag taacagttag tgaagtagag
    25501 gaaagcaact gggcaacagc ctggaagaaa tactatcatc cggtgcgtat tacgcgaatg
    25561 ctgacggttg cgccaagctg ggaaaactac cagacagaag atcctctaga aaaaattatc
    25621 acactagatc ctggtatggc atttggcaca ggcactcatc ccacgacttc cttgacattg
    25681 acagcactag aaacagtctt gcgtggtggt gaaacattgc tagatgtggg aactgggtcg
    25741 ggcgtgctaa gtattgcagc aaaatatctt ggtgcaaaag atgtctatgc ctttgattta
    25801 gacgaagtag ccgttcgttc ggcgaaagaa aatatggata tgaatgaaat agccaaagat
    25861 gtccatgtat cagctaacga tcttttgaca ggaatcgaga ttgaaagtga tgtaatcgtt
    25921 gcgaacattt tagcagatat catcttattg atgatcccag atgcttggcg tttactgaag
    25981 caaacaggca cgctgatcgt ttcaggaatc attgaagaga agaagcaaat ggttctggat
    26041 gccatgttag aacaagggtt tatcgtggat caaattttcc agcaaaaaga ctggtatgca
    26101 attattttga aaaaaccaga ggtggaatag atgcagcggt attttttaaa agaatcatat
    26161 gaatcaaaag actattttgt tttagaaggg gaaaactatc accacattgt acgggttatg
    26221 aggatggaac ccgaggatga agtttttttg gcattcaatg atcaaatagc cattgttgca
    26281 gaaatcacgg agattactga agaggctgtt tatctgaaag aaattaaaaa agaagaaaat
    26341 caaaaggaac tgccaagttc gattaccata gcgagtggct atcctaaagg ggaaaagctg
    26401 gattggatcg ttcagaaatc cactgaatta ggagcgcaag cttttcttgg tttcccggca
    26461 tcagcctctg tcgttaaatg ggatgcaaaa aaacgtatga aacgacaaca acggcttgaa
    26521 aagattgctc aagaggcagc ggaacaatcc catcggcaga tcgtgccatc agtagagtta
    26581 ttggaaacga aacaagattt atttgagcgg ctagatgatt atgacgtggt cttggttgct
    26641 tatgaggaat cagcaaagca aggcgaaaaa tcaaatgcag ccaaaattct tagtgaaaca
    26701 aaaccagata gtcgaatctt agctgtcttt ggaccagaag gtggattatc ccgcgatgaa
    26761 gtcgattatt ttgtagaaca caaagggata ctttgtggat taggaccaag aatccttcgt
    26821 acagaaacag cacctcttta tttattaagt gctgctagct atcaatggga attaatgaat
    26881 ccttctttca gctaacgttc ttcattaata aaagagtatc gacagaaaga attgaaaaaa
    26941 tagtcaacaa tttgtataat agaaacaatt atttatacaa tggatacgaa aaatcgtttg
    27001 aaaaagcttt taaaaagcga acatttccgt aaaaaaacgt ataactgcac ggattttggg
    27061 acaattatca aacagatatt tgtcccgatt cccgttttac cgaaaaaaga aagggggatc
    27121 gattcctgtg acaaaagaag aaattttaac gggacccggt gtcataaagc tcgttagtca
    27181 atacatgggt cccgaacatg tagcatttgt tgagaaagca tgcgaatacg cgactgctgc
    27241 acatgacgga cagtttagaa aatcaggcga accctacatt attcatccta tccaagtcgc
    27301 aggtatatta gcagatttaa aaatggatcc ccatacagtg gctacaggct tcttacatga
    27361 tgttgttgaa gatacagaaa tcactttaga agatctgaga gaagaatttg gcgatgacgt
    27421 tgctatgtta gtggacggcg taaccaaatt agggaaaata aaatataaat cccacgaaga
    27481 gcagctagca gaaaaccacc gaaagatgct gctagcaatg gctcaagatt tacgagttat
    27541 catggtcaaa ttagctgata gactacataa catgcgtacg ttgaagcacc tgcgagaaga
    27601 taagcaaaga aggatcgctc aggaaacttt agaaatctat gcgcctcttg cacatcgtct
    27661 agggatcagc cggatcaaat gggaattaga agatacagca cttcgttatc taaatccaaa
    27721 acagtattac cgtatcgtcc acttgatgca gacgaagaga gaagaacgtg aaaaatacgt
    27781 aagtggtact gttgaagata ttcgaatagc gacagaagag ctggggattt ttgcagaaat
    27841 ctatggacgg ccaaaacaca tttattcaat ctatcgtaaa atgaaagatc agaaaaagca
    27901 gttcaacgaa atttatgact tgctagctat tcgagtgatc gtagattcaa tcaaggattg
    27961 ttatgctgta ttaggagcaa tccacacaaa atggaaacca atgcccggca gatttaagga
    28021 ttatattgcc atgccaaaag ctaatatgta ccaatctttg catactaccg tgatcggtcc
    28081 ggcaggaaat ccggtagaaa ttcaaatccg aacacaggaa atgcatgaaa tcgctgaatt
    28141 cggggttgct gcacactggg cctataaaga aggaaaaaat gaaaaagtag aaccagatgg
    28201 tatgacgaaa caattaagct ggttccatga gatactcgaa cttcaagacg aaagctatga
    28261 tgcttctgaa tttatggaag gcgtaaaagg agatatcttt agtgataaag tctacgtctt
    28321 cacaccaaaa ggagacgtta ctgagttacc aaaaggatcc ggaccattgg actttgcata
    28381 cagtatccat acagatatcg gtaacaaaac cactggtgca aaagtaaatg gcaaaatggt
    28441 gcagcttgat tacaaattga aaaacggaga tatcattgag atcatgactt ctccaaattc
    28501 atttggccca agtcgcgact ggttgaaatt agttgctact agcaaagcaa gaaataagat
    28561 caaacgtttc ttcaaagccc aagatcgaga agaaaatgtg atcaaaggcc acgaatccgt
    28621 ggtcaaatgt attacagatc taggatttac gcctaaagat attttgacga agaacaaact
    28681 gcaagaagca ctcgatcgtt ttaattatca aacagaagat gatctctatg cagctgtagg
    28741 gtatggagaa gttagcccct tgacgatggc caatcgtctg actgaaaaag aacgtaaaga
    28801 acaaaaaatc gagcagcaaa agcaagaagc agaagaaatc atgaatcagc cgaaaaaaga
    28861 acctgacaaa atgaaagtac gtcatgaagg tggcgttgtc attcaagggg tagaaaactt
    28921 actaattcgt atcagtcgct gctgtaatcc gattcctggt gatgatatcg ttggttatat
    28981 cactaaaggc agagggatat ccattcatcg tcgagattgt ccgaatgttc agcctgacaa
    29041 accaaatgta gcagaacgtt tgattgaagt cgaatgggaa gatacatcga atacacgaaa
    29101 agagtatgat gcagatttgg aaatttacgg ctataatcgt tcaggcttat tgaatgatgt
    29161 acttcaaaca gtcaatgcgc taacgaaaaa tctcaacagc gttgaagcac ggacgaataa
    29221 agataaaatg gcgacgatcc atttgacggt tggtatccag aatttatccc acctaaagag
    29281 tatcgtggat aaaatcaaag cagtacctga tgtctacagt gtacgccgga cgaatggata
    29341 ggaggacaga tatgagagca gttgttcaac gggtaagtaa agctgctgtg tcagtcgatc
    29401 agcaagaaat tggaaaaatt ggctacggcc tacttgtttt attaggcatt catgaaaccg
    29461 atacgcaaaa agatgtggac tatctggtaa aaaaaattgc acaaatgcgt atttttgaag
    29521 atgaacaagg aaaaatgaat attagtgtag aagatgtggg aggacaaatt ttaagtatct
    29581 ctcaattcac actgtttgcg gatacgaaaa aaggaaatcg accaagcttt gtggaagccg
    29641 cacgaccaga aacagctatt cctttatacg aatcattcaa tgaaggaatc agaagtcgcg
    29701 gcatcacagt cgctaccgga caattcggag cagatatgtc ggtttctctc gtcaatgatg
    29761 gacctgtaac gatcattatt gattctaaaa acaaataaag taatctggtt ttcactagta
    29821 aaaactatga aacacgtcgc ttaagttaga ctgataaact ctaacttaag tggctttttt
    29881 gtctgcaaaa agcgcttctt ttgtcagcag tcggaaaaaa agttaagctt aaattataca
    29941 agttattttt tataggaggt tgctgaagat gaaagaagta ccagtagtag atatcaaaat
    30001 cacagattta aaaaaattgt atcaaaaagg atacaacatg ttggaggagt tacgccatga
    30061 agcggacgct cccgtcgtca aagcaaagat tttcaataaa gaagctatca cgatctacgg
    30121 atcatcagca gcaaaagtat tttatgatcc aaggaatttc aaacgaaaag gtgctatgcc
    30181 aaaattagta ttgaagacac tttttggtca aggtggagta cagactttgg atggagctgc
    30241 tcatcaccat cggaaaaata tttttatgga tctaatgaca ccggaaagaa tggaagatta
    30301 tcatcgtatc ttagataaga atctgacaca agcacttgaa gcacagcatg gtcaatttga
    30361 actatttgat ttaagtaaga tggttttctt tacatctatt tgcgagtggg caggtatcaa
    30421 tttatcagcg atttctaaag atgaggtcga aaaattagca gaatatcaaa tctctatgat
    30481 cagcggaacg tttacttctc cgattgatca cataaaaggt gtcgaaaatc gcaaaaaatc
    30541 agaaaaatgg gcgcaaggct tgattgaaga agctcgacaa aatcctgtgg ctggaaaaga
    30601 aaatgtggca ttatatgctt ttgccaatgc cacagattta gatggtcagc ttttacctct
    30661 ggaagtggca gctgttgaat tattgaacat catccgtcca acggttgctc taactgtgtg
    30721 ggcggcattg atgggacatg cacttttcag tagaccagat ttgtatcagc aattaaaaaa
    30781 tgacttttcg acgttacaag atccgtttat tcaagaaatg agacgctatt atcctttctt
    30841 cccaatgcta cctgcgattt cgctgaaaga agtagaagta gatggttatc gtataccaga
    30901 aggcagttgg gttattttag atttatatgg aacagaccat gatgaacgaa cagttgaagc
    30961 gccagattcc tttatgatca aacggtatgt agggaaagca aaagatattt cctataaaga
    31021 agaatatgag atgattgcac aaggcggggg aaattttcga caaatgcatc gctgtgcagg
    31081 agaatggatc actcttcata gtctacgtgt gttttccgat cagctagtaa ataagtttga
    31141 gttctctgta cctgaacaag attggacgat tccattcaat cagtttccga cttatccgaa
    31201 tagccgagcg ctactttaca agaattagat tagcataaac ttatcgaaaa aaaggaagat
    31261 caaacagtgc tgatcttcct tttttcatat tactataaat tgataaggtc aattgatcaa
    31321 aatccacgaa atgcttcatc aatggtttga taatcttcat tggataaatg caaatctaaa
    31381 gcttggacat tgtcagatac ttgctctgga cgcttagctc caggtataac tacagacact
    31441 cgtggattct tcatgtacca agctaaaaca agttgcgcag gagtggcttg gtatctttta
    31501 gcaatggggc gaagtacatc gacagcggtt aaaatttctc tgaaacgttc tccttggaaa
    31561 tctggatttc tttttcgagg atctccttca ccaaattgtt tttcctcacc aagttcatat
    31621 tttcctgtca gcaaaccgga agcaagagga aaatagggaa caaaactgat tttgtttttt
    31681 tcaagataag ggaataattc tttttcagcc tgccgatgga tcaaactgta tttatcttct
    31741 actacatcga catatccatc tgcattcgct tctttcaatt gttctaacgt gaagtttgac
    31801 acaccgactg ctcggatttt tcctgcttct tttaattcgt gcaatgtagc aactgattca
    31861 ttttttggtg tgctttcatc cggaaagtgg atataaaaaa tgtcgatgta atcagtttgc
    31921 agacgtttta gtgcatcttc caccgattgt ttgagaaact caggagaatt atcaaatgtt
    31981 cgccctttat ttggtacatg agcagctttt gtagcgatga cgacacgtga gcgatcatat
    32041 tctttttcct tcagtacctc gccaatcagt tcttcggaac ggccgtttcc gtaagcataa
    32101 gcggtatcga tcaaattgat cccgctattc aaagctgtac gaactacttg ttttcctgtc
    32161 tcatcttcca aaccgtcaaa aagattatgt cctccaacgg catttgctcc taatccaagc
    32221 tgttctgcgt agacttgtga atgtccgatt cttatttctg ccatagctga ttcctccttt
    32281 ttgtctagtc taaaacaaaa taccaaaaag ctgaactaaa acgtttttgt gaggggaaat
    32341 agtcagatat ctcttgactt tgagactggt ttttagtagg ttataagtaa tatcgaatgt
    32401 caatgaaaga gcgagtaagt ttttgaaact ttacaagaga ggatcatcga ggctgaaaat
    32461 gatctaaagt ttcattagcc taaagacact ctggagatgg acgagtgaaa agccgtcccg
    32521 cgtacgagcg ttattcgtaa gaggaaagag aaatctttca aaattaggtg gtaccgtgta
    32581 tgaatttcat tcacccttgt tcatgatgaa caagggtgat ttttttattt acatagctaa
    32641 gaaagggaga gaaaactatg agtttccaaa gaccaaaagg aacaaatgat attttgccag
    32701 gcgaatcaga aaaatggcag tttgtcgaag aaacagcacg tttattattc agagattatc
    32761 agtataatga aatgcgtaca ccgatttttg aacactatga agtgatatcc cgaagtgtag
    32821 gggatacaac ggatatcgtt tcaaaagaga tgtatgattt ttatgacaaa ggggaacgcc
    32881 atgtgacgct tcgaccagaa ggtacagcac caatcgttcg ttcttttgtt gagcataaat
    32941 tgttcggccc agagtatgcg aaaccgttca aaacttttta tatgggacca atgtttcgct
    33001 acgaacgccc tcaaaaagga cggttacgtc aattccatca aatcggagta gaagcatttg
    33061 gatcagaaaa ccctgcaact gatgtggaga gcatggtgat ggcacttgat ttcttcaaac
    33121 agttggggat cacgcaaatc cggctagtca tcaattcatt aggtgataaa gaaacgcgtc
    33181 aagcctatcg tcaagcattg atcgactatc ttttaccttt tgaatcagag ctgagtgaag
    33241 actctaaacg ccgccttcat gaaaatccat tacgcgtatt ggacagtaaa gacaaacgag
    33301 atcaaaaatt tgtggcagaa gctccatcta tcttagatta tctaagcgag cctgctcaaa
    33361 aacattttga taccgttgtt tccatgctgg atgcattggc tgttccttat gaaatcgaca
    33421 gtaatatggt tcgcggacta gattattaca cacatacgat ttttgaaatc atgagtgacg
    33481 caccaaaaat gggggcacaa gcaacgattt gcgcaggcgg acgttatgac aatctagtgg
    33541 aagaactagg tggtccttca acacctggct tcggatttgc aatgggtatc gagcgattac
    33601 tcttgactat ggaggcagag gaagtagtta tccctgcatt taatgagttg gatgcttacg
    33661 tggtcgcact cggagacgaa acaaatatcg aagcattgaa agtggtccaa gcgattcgaa
    33721 actttggttt ttcagctgac cgcgacttca tgaaccgtaa agcaaaagcg caatttaaga
    33781 cagcagataa agcgaatgcc aaattagtat tggtgatcgg tgaagatgag cttgctagtc
    33841 aaacgattaa tgtcaaagca atggcaagcc gaaaagaaaa aagttttcca ttagaagaga
    33901 tatatgaaca ttttgatgaa gtctatgatg aaatgacttt attcgagtag gaggaaaaaa
    33961 ataaatggca caaagaacga tctattgcgg attagtttcc gaacaacaat tagaacaaac
    34021 agttacatta aaaggatggg tacaaaaaag acgagacctt gggggagtaa tctttatcga
    34081 cctacgtgat cgtgaaggaa tcgttcaaat cgtctttaat cctgaaatca acaaagaagc
    34141 atgggaaatc gcggataaat gtcgcagtga atacgtgatc gaagtgactg gggaagttag
    34201 aagacgcgga gaaaatgcag taaatcctaa aatggccact ggtgaattcg aagtgattgc
    34261 aagtgaaatc gtgattttga atcaagcaaa aaccccgcca tttcaaattg aagatgatca
    34321 agcaatcagt gacgaaatac gtttgaaata tcgttatttg gatctgcgtc gtccaaaaat
    34381 gactaaaaac atcaaattaa gaaatgcgac aacaaaagcg attcgtcatt atttagatga
    34441 taatgacttt ctcgatatcg aaacacctta cctagggaaa tctacaccag aaggcgcaag
    34501 agactaccta gttccttcac gtgtccatgc tggacatttc tatgcgttgc cgcaatctcc
    34561 tcaattgttt aaacaattgc tgatgaatgc tggattcgac cgttattatc aaatcgttcg
    34621 ttgtttccgc gatgaagatt tacgtggaga ccgtcagcct gaatttacgc aagtagatat
    34681 tgaaacaacg ttcttaacag cagaagaaat ccaaacttac acagagggat tgattgcgaa
    34741 ggtaatgaaa gaagttcgcg gtatcgaagt cacattgcca tttcctcgta tgacctatga
    34801 tgaagcaatg gctcgctacg gcagcgacaa accggataca cgttttgata tggaattgat
    34861 cgatctaagc gatacagtca aagaagtaga attcaaagtc ttccaaatgg ctcttgaaaa
    34921 cggcggtgtc gtgaaagcat tgaatgctaa aggcgctgca gatcgctact cacgaaaaga
    34981 catggatcag ttaggtcaat atgtaggaca attcggagct aaggggcttg catggctgaa
    35041 agtagaagaa gacggattga aaggtccaat tgctaaattc atgggagaag caacagaagc
    35101 aatcattaaa gcaacagatg caaaaccagg tgacttgctg atgtttggcg cagataaaag
    35161 tgaaatcgtc gctgctgcat taggtgctat ccggacacgt ttaggaaaag aattaggact
    35221 aattgatgaa tcgaaattca atttcttatg ggtaactgat tggccacaat ttgaattttc
    35281 agaagaagaa ggtcgatatg tctctgctca ccatccattc acgatgccaa aagaagaaga
    35341 tatcccgtta ttggctgaag accctgcaaa agtctatgca gaagcttatg atatcgtctt
    35401 gaacggttat gaactaggcg gaggttctat ccgtattcat actcgtgagc ttcaagaaaa
    35461 agtactagaa actctaggct ttacaaaaga acaaatggaa gaacaatttg gtttcttgtt
    35521 aacagcttta gattacggat tccctccaca tggcggtatt gcattaggat tggaccgttt
    35581 tgcaatgtta ttagccggag aagataatat tcgtgaagtc attgcctttc ctaaaaacgg
    35641 aaaagcagca gatgtgatga gtgatgcacc aagtatggtt tcgcctttac aattgttcga
    35701 attgaatatt gatgtaacga atatagaaga atagccgata aaaaggtaaa aaggatttgt
    35761 cgaatacgct tcttttgaca accttgattt aattgaaaaa cactttataa tgaagccata
    35821 acatataaag cggggctttt ccatgaaaaa aacattcgaa aattgggctt atcatgatta
    35881 cacgaccaag ctttcggtat ctatcgttta tgcgttgatc gcatcagtcg cattgaattt
    35941 cttttatcag ccaggacata tttattcaag tggtattact ggtctagctc agattttgac
    36001 gactttgtca aaatcactga taggattcga agtaccggta tcaacaactt tgtatttact
    36061 gaatatacct ttgtttgtat tggcgtggtt aaaaatcggg aagaagttta caattttcac
    36121 ttttatcaca gtcactttga cctccctttg tatccatatt ttaccggaaa cagtcctatc
    36181 taccgatcca atcatctgtg cgttgtttgg gggggctgtg atgggactag gtattgggct
    36241 agcgttgaga aacggcctat catcgggcgg tctagatatc gtgagtatca cgattcgtaa
    36301 aaaaacagga aagtcgattg gttctatttc gatctacttc aatgcattga tcgtttttgt
    36361 tgccggaatt ctatttggct ggcaatatat gttttacagt acgttgtcta ttttcgtcag
    36421 tggaaaagta actgatgtcg tttatactaa acaaaagaag atgcaggtaa tgatcgtaac
    36481 gaaacatcca gaaaaagtag taaatgaaat ccaaaactgt atgcgtagag ggatcacgat
    36541 catcaatgaa gcagaaggtg cttataagca tgataagcag acagtattga tgacgatcgt
    36601 tacacgatac gaactgcctc tacttagaga agcaatgaaa gcttctgatc cacaagcttt
    36661 tgtaagtatt gcagaaaacg tccagatact tggtcgtttc tatgaggatg gcatttagat
    36721 cgagtttgac aaaaattcat gaaatactgc tgtagacaga cgttcgtttg tctgcagttt
    36781 ttttctacca tagtagaatc agcaaagaaa aactgctttt ttatttgaaa ttttcttaaa
    36841 gttgaaaaaa agcatgttta tttttttaaa tttatgggat gatagaaaaa aatagtagag
    36901 aaaggacacg attctacttg ctattttcac ttaatacccg tatattacga atgaaaaaag
    36961 tttatgaaaa gttggaatat tgaatggaaa aacaaaaaag gttaaaaaac agccgctaca
    37021 tcactgggtt tgatgggctc cgttcattag ctgtggtggg tgttatcttc tatcaccttc
    37081 tgccaactag tatgaaaggc ggctatttgg gcgttccgat ttttttcgtc gtatctggtt
    37141 atttgatcac ggatctgctg cggcaggaat gggaacaaaa tggaaaaatc aatatttggc
    37201 aattttatgt cagaagaatg aagagactct atccggcact tgtttttatg cttgtgacag
    37261 cttcggctta tatcactttg tttcagcgag ggctgttaaa caatcttcga ggagttgtgg
    37321 tcagcagctt actgtatgtc aacaactggt ggcaaatcaa taacggttta tcttatttcg
    37381 atcgatttgc taatgagtca ccgtttacgc atatctggtc tttagcagta gaaggtcaaa
    37441 actatttggt ttggccgatc ttgtttgtat tgttgatggt atttgttcgt aaaagaaaat
    37501 ggatctttta tactgtgtta ggtacatcct tcctctccgc catcttgatg atgatcttgt
    37561 atacaccagg aggcgatcca acccgtgttt attacggtac cgacactcgt ctgttttcta
    37621 tatggttggg aagtgcgctt gcgtttgttt ggccaagtac gcgtttaaag aaaaacatcc
    37681 cgaaacaagc gaaaaatatt ctgaatatcg ctggaggaat ctcgcttttg gcactgattc
    37741 tttgcttctt ctttttagat gatcattaca atttcatcta ttacggagga atgctcctag
    37801 tgagtctgtt atgcgtagtg ctagttgcag tgacagcaca ccccggagca agtttgaatc
    37861 gatggctgac aaatccagta tttacttgga ttggcaaacg aagctatggc atttatctgt
    37921 atcagtttcc agttatgatt ttttatgaag caaaggtgac tgatattgcc gatcatgttt
    37981 tgttgcatac tttagtggaa atcacattga ttctaatcat cagtgaactt tcttatcgat
    38041 atgtggagcg tccgctagca cgttttgatt atggtcgtac attccaagtg atgaaaggat
    38101 ggttcagcaa accgatcatc agtaagagca aaccatgggt tgttccaggc acattacttg
    38161 tagcaattgc actcgttggt ttcgttacgg caccgaaaaa cgctgtaaca gccgatcaaa
    38221 aacaactgca ggcaaaaatc gctgaaagta aaaaattagc agaagaaacc agaaacaatt
    38281 cagaaaacac cactagttcg gttgaccaag cgatccttga taagtacgag ttgtccaagg
    38341 cacaagggaa taaggcacaa aaattagaac taacggcatt tggtgattca gttatgttgg
    38401 atgctgcaac ggatctacaa gaactttatc caaaagtagt ggtagatgga gatgtcggac
    38461 gacaattata tgccagtgta ccgtacattg aaaaattaaa ggaacaagat ttgttgaaag
    38521 acactgttct gatcggacta ggaacgaatg gctcattctc ggaagctcag tttgatagtg
    38581 tgatggatgc aatcggtgat cgaaaagtat actgggtcaa tgtccgagta ccgacgaagc
    38641 gctggcaaaa tgacgtgaat gcgatgcttg aacagatgac gaaaaaatac gacaatatga
    38701 cgctgattga ttggtacagc tacagtaatg accatagtga ctggttttat gatgatcaag
    38761 tccatccaaa tcctgaaggg atgacacatt acattcactt agtcagcgaa gccttgctag
    38821 gaggaagttc aagtacgaac agcagtgaga caaacgtgac agaatgatag ctacaaaaaa
    38881 cttcaggcaa aaacatttgt ttttgcctga agttttttta tatttaccgt gtttttaaca
    38941 ttcatataga ttcaggaagc aaaacggttg agtgtgtctg taaataacgt tctttttgtt
    39001 ttcgggtgag ttttttgaat gcagcttctg cttttgttgc ttcgcttctt gaagaaaata
    39061 cttctgcatg gatcatctta acaggtcttc ttttttgaag acgagtatat ttagcaccag
    39121 taccattgtt atgttcctct aatcgtctag aaagatcggt tgtatatcca ccataaaatg
    39181 tctgatcttt acagaataat acgtaaaaat aatgttcatt tgccatagag catctcacga
    39241 atctctgcag tgtattctcc atccatattg taagtgatca atggtggagc gactttgaat
    39301 ccgtctaatt tgccttcttt gattccttca actaacaaaa tatttgcctc ttttccaggt
    39361 tttgggtaaa caaattggat ccttttaggt gcaattcgat agtcttccat gacctgaatg
    39421 atttctaaga accgatcagg acgatggacc atagtaaagc gtccatttgt ttttagtagc
    39481 ttacttgaaa tatcgatcac ttcttctaac gtcgtatgaa tctcatgacg ggcgattgcc
    39541 aggtgtttat tagggttcgt tttatttgtt ggcagccctt tgaaataagg tggattacag
    39601 acgactaagt cacaagaatt atgtcgaacg acagataagc tgtctttcag atcgatggtg
    39661 tggacagtca tttgttcatt taaatggtta agcgcgatac tacgtcttgc catgtctgct
    39721 agacgaggtt gaagttcgat agcgtcgata gccgcctggg ttcgtttact taaaaaaagt
    39781 ccaactgcgc catttcctgc acaaaggtca acaatcttcc cttttttagg tacacgggaa
    39841 aagtgagcaa gcagcacagc atctaaagaa aaagaaaaca ctttggaact ttggataatc
    39901 ttgatatctt ctgcgtataa ttggtcgatt cgttcgtctt cgtgaagcat ggattccctc
    39961 caaaatgaaa aaaatagtcg tctgtattat actattttta ttaaaaagaa ccaaggtggc
    40021 ttgtcaaacc tctatttttt atgcatacta tgaccataag aagggaaagg aagagactat
    40081 gttttatcga tttatgcgcg gggtagttcg agtactatta gccttagtta atggaaatgc
    40141 ccgctatgag aataaagaag ttttaccgca agatgagaat tacatactag ttgctcccca
    40201 tcgtacttgg tgggacccat tatatctggc ggtagctgca gcacccaagg aattcagctt
    40261 tatggcaaaa gaagaactgt tcaaaaatcc agttctgcga tttatattaa ctcgatccaa
    40321 tgcttttcca gttaaaagag aaaatccagg accaagcgtc attaaaacac ctgtaaagat
    40381 tttgaaatcg acaaatttga gtttgatcat gtttcccagt gggacaagac atgctacaga
    40441 acttaaaggc ggaatggcat tgatttccag aatgtctaaa gcgaaaatcg tgccagctgt
    40501 atatcaaggt ccgttgacac ttggggatct gttcaagcgt cagagagtga cagtgcgttt
    40561 cggcgaacca atcgacttat cagatatcaa gaaaacggat aaagaaggta tggcggagat
    40621 cgaacgacga acacaagcag cttttgacaa attggacaaa gaagtgaacc ctgatttcaa
    40681 atatgaagtc aaaaaaaaat aaaatgataa gtttatgcgt gggcggtctt ttgaatctac
    40741 tgtcctgttc agttcagact ttttcaatga aaacgaggga gagacgtcaa gtctgccctc
    40801 tttttgcgtt ccaatatgta aaatagaaga caggtgtcat gtaatggatc aaagtgttat
    40861 aataattaga aggacatttt ttgaggtgat tgaatgcgtt ttttacatac agccgattgg
    40921 catatcggaa aaaaactaca aggttatgac ttgattgaag accagcgtca tgtactatct
    40981 tctatactag atatcgcgaa agaagaaaaa gtagaggcaa tcgtgattgc cggagactta
    41041 tatgatagga gtgtcccagc tgttgatgcc gtagaattat acaatgaatt gatggtcgat
    41101 tggaatttaa aggaaaagat cccgattttt gctatatcag gaaatcacga tagcagtacg
    41161 agactttcag cgggtacgcc atggttcagt tattcgaact attttttgcg aacacagcta
    41221 gcagaagcat ttgaaccagt cgagtttaaa aacgtacaat ttttctttct tccttacttt
    41281 gaaccaattg cagcgagatt atattttgaa gatgacaata tccgaacgat caagcaagcg
    41341 gtagccaagg tgatagataa aatgcaggag ctatttgatt cagaaaaaaa acaagtattg
    41401 attagccatt tttttgtaat gggaagtgta aaatctgatt cggaaacgaa aatcgaagta
    41461 ggcgggttgg attcgatccc tggtgaacta cttacaccgt ttgattatgt agcactagga
    41521 catttgcata atcaagctgc tttgagacag gataatgctc gctatagcgg ttccccattg
    41581 aaattctctt tatctgagat caaccaaaaa aaaggcgtat ggatcgttga cctgacaaca
    41641 ggcctggagt tgaattttag ggagatcgaa cccctgcatg atattgaaga aataacagcg
    41701 agtttcaaag aattgctgga tcctgccttt tatcagtccg tcaatcggga gaattacatc
    41761 tatatctatc tcactgatag aacgatcatc ccgaatatga tgaaccagct tcggcaaata
    41821 tatcctcgta ttttggggat tgaacgggca aacggtcgag cagaacaaac aaagcgcaaa
    41881 cgaaaagtac tagaaatgaa agatcctcaa aaattagcag aagcattttt tagtgaaatg
    41941 acggaagagc agctaactgc aaaccaagaa aaatggctcg gagaaacgtt gcaagaaatc
    42001 atggaaatgg aatgatcaag aatgaaaccg aaaaaattac aacttaaaaa ttttggtcct
    42061 tttatcaatg agaccgtaga tttttcacgt ttgacagaag caccgctttt tttgatcagc
    42121 gggaagacag gagctggcaa aacgacgatt tttgatggga tcaccttcgc actttttggt
    42181 gaaacttccg gaagacttcg ctctggaaaa gagatgcgtt ctttgtttgc tacaccagac
    42241 gaagaaacaa gcgtgacttt ttcttttgaa catcagcaga tgagctatga aatcgaacga
    42301 aaaccagaac aagttttagc aaaacgaaaa ggaaacggaa caaaaaaaca ggcagccaaa
    42361 gtaacgttga ccatttttga tgggaaagga aaagaaataa aacaaatcac aaaacgtaca
    42421 gaagcagatc agttgatcag agaactgatg aatctcgatg cgaaacagtt ttctcaaatc
    42481 gttttactac cccaaggaga attccgtaat tttttggttt catcaagcag tgataaagag
    42541 actgttttac gtcatttatt cggtactcaa tttttccaac agttcaatga gcgattaaaa
    42601 gaaaaagcaa aacaacagca acaaaaattg gatcatttag agcaagagct gcatcttctg
    42661 caaaaaaggt ttgttttagt caaagaagag gaaccagcag cagggtttga agccgtttta
    42721 gcccaatgga caaaccatca ggcaatactc gaaaaacaaa tcacggaaga aaaagaagct
    42781 ctaacggaac ttcaggaaaa acaaaagcaa ctagaaaatt cctactatcg ttttgaaagc
    42841 ttgaaaaaaa gttatgaaga aaaaacgata ttaataaaca agcaaaagga attagctgct
    42901 caaaaagacg aaatagagga aaagaaacga tggattgggt attatgaatt tacgcaacga
    42961 ttagtcgaac caatccagcg cctacaagaa ataaaagttg aaaaaataga actgtcacaa
    43021 aaaagtagtg agcaaacaaa gaaactagcg atgactacgc agaattatga agagtggcaa
    43081 aaaaatgaag caaaccgcgt acgccgacaa acaaaaatca aagaagctgc tcaaaaactg
    43141 caaaacaaac agatgatggt gccaattgtc gaatccttac gtagtaaaaa acaagacgca
    43201 gaacaattaa caatttcatt ggataaagaa gaattgaatg ccgagaagct gtatcaaaat
    43261 ctcgctatct atcaggaaag aactcgaatt cttcaagaaa atttactgga tcaggaaaaa
    43321 ctgcaagaag atcgtcttgc ttttggacag ttaaaatatc tagatgaaaa gcagcaagaa
    43381 atagaaaaac aaaaagcaaa agacactggg tcgctcgcac agataagaga agaactcaaa
    43441 aaagcaactg aagaaataac aaaaaatagt cagctattga aagaacagac agaacaagca
    43501 gaacagctga aaagccaatg ggcaaagcag caaattgctc gtcttcagct ttttttgcgt
    43561 ccaggggagc catgtcttgt ttgtggatca acggaacacc ccatacaaca attaaaacac
    43621 gaacagccaa ataaggaaga tatcttaaaa gtagaacaac aactggcaga agctgagtct
    43681 gctgtagaaa gaactcagca aatcattgcc aaaagtgaag cacatgaaaa taatcttcaa
    43741 caacaattat cagatttaga acaaaaaatc atggcaacga atatacaatt ggctgaacaa
    43801 gaagaacact tgcaacaggc tttgaaaaag gcatttcctg agataacaga acaaaaaaca
    43861 aaaacaactc ttgtagtaat cgaacaatct ttagatagac gaaaaagcaa gatggccaat
    43921 gcaagaatag agctggaaga aataagacaa caagcagaaa agctcgaaca acaattagct
    43981 gtctacaaac agcagcagac agaaaaaaat aatcagctcc agcaactgca aggagaaatc
    44041 aaaagtttag aacaacaatt gcaagatacg gatagtaact gtttagaaga ggagatacga
    44101 cgacttgaaa aacagttaga agaagactcg aatcaactag ctgttgagca gaaagcagga
    44161 gtgcaattgt tgcaagatct tcatacgctt cggacacagc ttgatcttca acatgaacaa
    44221 atagaaaaaa tagatgaaaa aatagaaaag gaacaagaaa gaatagctaa aaagttagaa
    44281 agccagtcgt atttttcaac aattgaagaa atctgtgcat ttgcacaaca gcaagcacaa
    44341 tataaagaaa accaacgtac agttacttct tatcatgaag agcaattagt gacagctgac
    44401 cggctgcgtc aattagaaaa tatccagcta actgagacat tcccagatac agaagagatc
    44461 agccaacagt tagaagaaat aagacgtatg atcgaaataa agcaagaaaa gatatacggt
    44521 ttacaagaac aaaaaattag taatcaaaaa atctacgatg aatgtctttc gatttatcac
    44581 acaagccaga cgaaattaga tgagcttacg caattacagc agctttcgca aacattcaac
    44641 ggagaaaatc ctaagaagac tagtttggaa cggtatgtgc ttcaggttta tcttcaagaa
    44701 gtgttacaag tagcaaatga tcatctccaa cgtttaacga aaaatcgtta tcaatttgag
    44761 ctagcggata ccattggcag ttatcgaggg aaaaccggat tagaaatcaa tatctatgat
    44821 gatcaggcag gtatgtctcg aagtgcgcat accctgtctg gcggtgagag ttttattgca
    44881 gcactttctt tagcattagc tcttgccgat gtgatccaga cacaagctgg tggtgtagca
    44941 atcgatgcat tgtttattga tgaaggattt gggtcattag atgaagaagc tttggaaatg
    45001 gcaatggaag ctttagaaac gatcgaaagt gaaggaagaa tgatcggcat cattagtcat
    45061 gtaagagaat tgaaagaacg agtattacaa caaataagaa ttgatacaga aggtagcgga
    45121 caaagcaaaa tacgctatca acttggataa aaagggagat tggtaactat gaaatggagt
    45181 attccagaaa aagtcattgc acgtggacgt caatacttgc aagaaggccg agtgttatcg
    45241 gttgtccctg accaagaaga aaacgtgtgg catgcagaag tcttgggcag tgaattgtat
    45301 atggttgatt tggatggtac agcaaaagag atagattact gccaatgtcc ctattgggag
    45361 gaacataaat attgtaagca tacagttgct gtggaacttt atttacggtc gaaaaatcta
    45421 tctcgtatca tgaaaaaaga ccagccaatg ctagaaccgg taaaagcaac aagtgaggga
    45481 gaaatcattg caaaaggttt ttctcgtcta aaaacacaaa acaatcctca agcggtgcaa
    45541 cctttgatta ttgaatacca agtagaaacg attgagacta atcagtatca cccagagctt
    45601 tctattttgg cactttactt gcgtatcggt tcattggcat caccgaaaaa aacatatatc
    45661 gtaaaaaaca tctatgattt tttacaagct tacacagaaa aagaaactta cactgttaat
    45721 aaacagtacc agtttcgtct agataaacgg gcgtttcaaa aagaagatca aaaaatactc
    45781 acgcatcttg caggaagtgc tcagacgcat caattattag gtacgaacgg cttacaagtc
    45841 aaaggaaaac tagataaaag atatctgctt ctcccagtag aacaagcccg cttcctgtta
    45901 gagcagatga atcagactac acgtttgcat ctgcagataa atgaacaaaa cgatcaagga
    45961 atacaatttt ccgatcaaaa caaaccttta tcattcaaag tggaaaaatt tggagaagat
    46021 tatttgctgc aagcattgaa tgattttgat tttttcttta tgcattatca gtggggagtt
    46081 atccaaggga cattttattc tctgacgaaa tatcaacaaa cggtttatca aacttggaaa
    46141 caattgattc gccgattgga gaaaccagaa gtagtgttca agaaaaaaga attaccgtct
    46201 ttgtttaaag agattatccc tctgctgtca gagatcggcg acgtagctat tgcccctgca
    46261 gtagaagagg aagtagcaga tatccccgtc gaatttattt tctttttccg aaaagcaaaa
    46321 ggaaaaatcc aagctcgagt cgatttcgtc tatggcgaag tcatctattc gactgacccg
    46381 aagcatgagg tctcgacaga acaggctttt caggtgttac gtgacacagt agaagagaaa
    46441 cgagtacttg atctattcag aatgtatcgc taccagaaaa atgaaactgg ttatgaacgt
    46501 ccattgccaa gcggggaaga actatatgcc tttttccgca cagaactatc tgtcttccgt
    46561 cagtttggtg aagtcagact tggaagaaaa ttgcgagagt tgtatttaga tgcgcataaa
    46621 catcaaccga aaatcgaagt aaccgagacc ggttcatggc tcgatattcg attcgatgtg
    46681 acagggatag aggagcaaga aatcgatcat gtcctgcaaa gcttgctgag aaatgatgct
    46741 ttttatacat tagaaaatgg tcaggttctt tcttttgatt cagaagaatt tcaacaaaca
    46801 agccaagtgt tgcaacaatt acgggaatcg atacgtacag aagaaggaac gatccatgta
    46861 cctaaaaatc aaggattgat cattcaaaat caattggaaa aaagcaatgc tacttttagt
    46921 gaatcgttcc aaacgatggt acaagatctc attcatccag aaagatacca ggcacagctt
    46981 cccaaaggat tgaatgcaac gatgagagat tatcaaaagc aaggttttcg atggttgaag
    47041 atgttaggtc attatcagtt tggcggtatt ttagccgatg aaatgggact tgggaaaaca
    47101 ctacagacaa ttgccttttt actttcggaa aaagaagaac ggaaaagctt ctctgctttg
    47161 atcgtagcgc ctgcgagctt aatttacaac tggcaggcag aggtgagaaa attcgcccct
    47221 tctttaagta tccaagtaat caatgggaat aaaaaagaac gtgaagaatt actggctaaa
    47281 gacacagata tccgagtgac ttcttatgcc agcttaagac aggatctagc taattatcag
    47341 tcgcaaaaaa ttgattattt gattcttgat gaggcacaaa tggtcaaaaa tagcagtacg
    47401 aaaactgcac aagcattgag agaattagct gtacctcagc ggtttgcact gagtgggaca
    47461 ccaatcgaga ataatttaga agaattgtgg tcattgtttg caacgattat gccaggattt
    47521 ttcccgacta aaacaaaatt cagggaactt gccgcagaag aaattgcgca aatgattcga
    47581 ccgtttattt tacggcgaga taaacaaact gtgttgaaag acttgccgga aaaaacagag
    47641 atgaatctat attctgcact tacagaagaa caaaaaactg tttatttagc ttacttgcgt
    47701 caaatgcgtg aagaaatcac atcaatggat tctgaagcat tcaagaaaaa ccggatcggg
    47761 atacttgctg gtctgacaag actgcgacaa atttgctgcg atcctcgtct gtttattgaa
    47821 gattaccaag gcggttccgg caagctcgaa caagtaaaag atctactggt agctgccaaa
    47881 gagaacaaac gaagagtttt gctgttttct caatttacag ggatgctgac tatcttgcaa
    47941 gaagagttag ccgagctagg gatctcgaca ttttatttaa gaggcagtac gaaaccacaa
    48001 gatcggttaa gtatggtaga cgcatttaat gctggcgaaa aagatgtttt tctgatttcc
    48061 ttaaaggctg ggggaactgg attgaatctc acgggagcag atacggtcat cttgtatgac
    48121 ctttggtgga atcctgccat tgaagaacaa gctgctggac gtgcacatcg gatcggacaa
    48181 aaaaatgtgg tagaagtgtg gcgtatgatc tctgaaggaa cgattgagga acgcatggat
    48241 tcactccagc aagaaaaacg cgaactgttc caaaaagtga tccaaggtaa tgaagaacag
    48301 ttgacgaaac tgactgaaga agacatccgc atgattttaa gtgtaggaga aatcgaggag
    48361 taagagactc gaaaaagcgt tcagtaatgg gtaagaaagc ctttctaaag aagcgattgt
    48421 ttatttgatt taaaaaatag caaggaggct gggattccat tttatcccag cttccttgct
    48481 atcatttttt tatttctatt tttaaaggtt atacaaagat tgtcttcatt gtctctaaca
    48541 gctgatcaaa agaagaaatc tcgatatcgc aaattggagt ctgttcaatt ttgcgttctc
    48601 gatggttgaa ccaaagcgct tgccagttag cactttttgc acctagaaca tcattgtcgt
    48661 aattatcgcc aacgtataag gtctcttctg gaagcatatg aaattctttc tcagctagct
    48721 gaaaaatctc tttttctggt ttttggaaac ctgttgcttg agaaatgagc atattgcggg
    48781 aaggaatcca attgttcaat tgaagttgct tcagtttttt tgtctgatgg tcagtcggcc
    48841 catttgtgat aattcctaat gggacattct tttccttcaa gaaatcgagg gtttttttga
    48901 cctcctcatg caagcagata ttatcaagtt cttcctcgta aattttttga aataagaggc
    48961 catcttcttc cgtaatatga gggtagtcta aatcttttag tgattggctg atccgatgtg
    49021 ctcgcatata ttctagtgtc caatctcctg ccattacttt tgggaagttt tcatcactat
    49081 gatggcggaa ccggatatat agtggatgca tatctgcatc tgaaacaagc ggaacgactc
    49141 ttttgaccgc gtttcgaaat ggctgctgct gatcatacat cgtatcatca acatcaaaca
    49201 caactgattt aatcaaaatt ttacacgcct ttcggttttc attgattttt tcacaaatcc
    49261 ttgactattg taacagaa
//
LOCUS       DAZUMV010000006        48772 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000006 DAZUMV010000000
VERSION     DAZUMV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.006"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..852)
                     /locus_tag="ACG8FK_000335"
     CDS             complement(28..852)
                     /locus_tag="ACG8FK_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS5095975.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(825..1505)
                     /locus_tag="ACG8FK_000336"
     CDS             complement(825..1505)
                     /locus_tag="ACG8FK_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5095976.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1528..2466)
                     /locus_tag="ACG8FK_000337"
     CDS             complement(1528..2466)
                     /locus_tag="ACG8FK_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5095977.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2711..3562)
                     /gene="ispE"
                     /locus_tag="ACG8FK_000338"
     CDS             complement(2711..3562)
                     /gene="ispE"
                     /locus_tag="ACG8FK_000338"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS5095978.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3716..6289)
                     /gene="mprF"
                     /locus_tag="ACG8FK_000339"
     CDS             complement(3716..6289)
                     /gene="mprF"
                     /locus_tag="ACG8FK_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5095979.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6430..7236
                     /gene="proC"
                     /locus_tag="ACG8FK_000340"
     CDS             6430..7236
                     /gene="proC"
                     /locus_tag="ACG8FK_000340"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS5095980.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7315..8871)
                     /gene="rny"
                     /locus_tag="ACG8FK_000341"
     CDS             complement(7315..8871)
                     /gene="rny"
                     /locus_tag="ACG8FK_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS5095981.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9282..10331)
                     /gene="recA"
                     /locus_tag="ACG8FK_000342"
     CDS             complement(9282..10331)
                     /gene="recA"
                     /locus_tag="ACG8FK_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS5095982.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10440..11681)
                     /locus_tag="ACG8FK_000343"
     CDS             complement(10440..11681)
                     /locus_tag="ACG8FK_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS5095983.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11921..12499)
                     /gene="pgsA"
                     /locus_tag="ACG8FK_000344"
     CDS             complement(11921..12499)
                     /gene="pgsA"
                     /locus_tag="ACG8FK_000344"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS5095984.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12708..13631)
                     /locus_tag="ACG8FK_000345"
     CDS             complement(12708..13631)
                     /locus_tag="ACG8FK_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS5095985.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14370..15656)
                     /locus_tag="ACG8FK_000346"
     CDS             complement(14370..15656)
                     /locus_tag="ACG8FK_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5095986.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15649..16917)
                     /locus_tag="ACG8FK_000347"
     CDS             complement(15649..16917)
                     /locus_tag="ACG8FK_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5095987.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17169..17630
                     /gene="mscL"
                     /locus_tag="ACG8FK_000348"
     CDS             17169..17630
                     /gene="mscL"
                     /locus_tag="ACG8FK_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS5095988.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17689..18135)
                     /locus_tag="ACG8FK_000349"
     CDS             complement(17689..18135)
                     /locus_tag="ACG8FK_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5095989.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18228..18473)
                     /locus_tag="ACG8FK_000350"
     CDS             complement(18228..18473)
                     /locus_tag="ACG8FK_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS5095990.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18588..19166)
                     /locus_tag="ACG8FK_000351"
     CDS             complement(18588..19166)
                     /locus_tag="ACG8FK_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5095991.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19243..19773)
                     /locus_tag="ACG8FK_000352"
     CDS             complement(19243..19773)
                     /locus_tag="ACG8FK_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS5095992.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19777..20637)
                     /gene="rlmB"
                     /locus_tag="ACG8FK_000353"
     CDS             complement(19777..20637)
                     /gene="rlmB"
                     /locus_tag="ACG8FK_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS5095993.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20630..21025)
                     /locus_tag="ACG8FK_000354"
     CDS             complement(20630..21025)
                     /locus_tag="ACG8FK_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS5095994.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(21022..22434)
                     /gene="cysS"
                     /locus_tag="ACG8FK_000355"
     CDS             complement(21022..22434)
                     /gene="cysS"
                     /locus_tag="ACG8FK_000355"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS5095995.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22431..22967)
                     /gene="epsC"
                     /locus_tag="ACG8FK_000356"
     CDS             complement(22431..22967)
                     /gene="epsC"
                     /locus_tag="ACG8FK_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS5095996.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23053..23292)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23289..24749)
                     /gene="gltX"
                     /locus_tag="ACG8FK_000357"
     CDS             complement(23289..24749)
                     /gene="gltX"
                     /locus_tag="ACG8FK_000357"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS5095997.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24919..26076)
                     /locus_tag="ACG8FK_000358"
     CDS             complement(24919..26076)
                     /locus_tag="ACG8FK_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS5095998.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26110..27480)
                     /gene="radA"
                     /locus_tag="ACG8FK_000359"
     CDS             complement(26110..27480)
                     /gene="radA"
                     /locus_tag="ACG8FK_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS5095999.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27543..28070)
                     /locus_tag="ACG8FK_000360"
     CDS             complement(27543..28070)
                     /locus_tag="ACG8FK_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS5096000.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28300..29106
                     /gene="proB"
                     /locus_tag="ACG8FK_000361"
     CDS             28300..29106
                     /gene="proB"
                     /locus_tag="ACG8FK_000361"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS5096001.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29103..30350
                     /locus_tag="ACG8FK_000362"
     CDS             29103..30350
                     /locus_tag="ACG8FK_000362"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS5096002.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30426..30770
                     /locus_tag="ACG8FK_000363"
     CDS             30426..30770
                     /locus_tag="ACG8FK_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096003.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30887..31213)
                     /locus_tag="ACG8FK_000364"
     CDS             complement(30887..31213)
                     /locus_tag="ACG8FK_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096004.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31828..33030)
                     /locus_tag="ACG8FK_000365"
     CDS             complement(31828..33030)
                     /locus_tag="ACG8FK_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5096005.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            33147..33662
                     /locus_tag="ACG8FK_000366"
     CDS             33147..33662
                     /locus_tag="ACG8FK_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5096006.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33861..34307)
                     /locus_tag="ACG8FK_000367"
     CDS             complement(33861..34307)
                     /locus_tag="ACG8FK_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5096007.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34354..35832)
                     /gene="msr(C)"
                     /locus_tag="ACG8FK_000368"
     CDS             complement(34354..35832)
                     /gene="msr(C)"
                     /locus_tag="ACG8FK_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5096008.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(36186..36815)
                     /locus_tag="ACG8FK_000369"
     CDS             complement(36186..36815)
                     /locus_tag="ACG8FK_000369"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5096009.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(36799..37263)
                     /locus_tag="ACG8FK_000370"
     CDS             complement(36799..37263)
                     /locus_tag="ACG8FK_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5096010.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37750..39018)
                     /locus_tag="ACG8FK_000371"
     CDS             complement(37750..39018)
                     /locus_tag="ACG8FK_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5096011.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39249..39986)
                     /locus_tag="ACG8FK_000372"
     CDS             complement(39249..39986)
                     /locus_tag="ACG8FK_000372"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5096012.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(40053..40622)
                     /locus_tag="ACG8FK_000373"
     CDS             complement(40053..40622)
                     /locus_tag="ACG8FK_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS5096013.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40750..41622
                     /locus_tag="ACG8FK_000374"
     CDS             40750..41622
                     /locus_tag="ACG8FK_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5096014.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            41964..42836
                     /locus_tag="ACG8FK_000375"
     CDS             41964..42836
                     /locus_tag="ACG8FK_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5096015.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            42833..44791
                     /gene="nagE"
                     /locus_tag="ACG8FK_000376"
     CDS             42833..44791
                     /gene="nagE"
                     /locus_tag="ACG8FK_000376"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS5096016.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPTAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(45123..45974)
                     /locus_tag="ACG8FK_000377"
     CDS             complement(45123..45974)
                     /locus_tag="ACG8FK_000377"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5096017.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            46097..46564
                     /locus_tag="ACG8FK_000378"
     CDS             46097..46564
                     /locus_tag="ACG8FK_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS5096018.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(46827..47309)
                     /locus_tag="ACG8FK_000379"
     CDS             complement(46827..47309)
                     /locus_tag="ACG8FK_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5096019.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(47409..48272)
                     /locus_tag="ACG8FK_000380"
     CDS             complement(47409..48272)
                     /locus_tag="ACG8FK_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5096020.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        13942 a        10443 c         8700 g        15687 t
ORIGIN      
        1 ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg caaccaatat
       61 tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga ttgttgctcc
      121 tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta gaaatcctac
      181 acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac cgattgctgc
      241 gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca tcacagcaat
      301 agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta gcccatccac
      361 gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata aaaagaacaa
      421 tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg taatggtcac
      481 gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc tcaaattcat
      541 caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga tttctgaata
      601 ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa cgactagcat
      661 ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg ctaaagagac
      721 atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca cacctagcat
      781 cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga attcatatga
      841 aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc cgcatactgt
      901 ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac atgcgcacta
      961 tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt tgttggttca
     1021 tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat tcgctgcttt
     1081 tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc tacagcttct
     1141 aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca gcggcctctt
     1201 ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc attgaaagat
     1261 gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt tttctcagca
     1321 atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac caaggttgac
     1381 ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac atgataagag
     1441 acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat ttcgatataa
     1501 cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag ctttcagatt
     1561 tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag tcaagctttc
     1621 taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct cagctacctt
     1681 cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca caaattcttt
     1741 tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg aaatcgctac
     1801 ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag tgacaaatgt
     1861 gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt caagcttttt
     1921 gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt tttcgcctaa
     1981 ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc aaacatgtgg
     2041 atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt ctgcttcttg
     2101 cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt tgctatccac
     2161 tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca cgaaaacatc
     2221 tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg gctccgttcc
     2281 agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg taaaatcata
     2341 catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt ccttgttcgt
     2401 atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca aaagatattt
     2461 tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat taacgtgaac
     2521 taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg atttaagcat
     2581 gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag ccatttcact
     2641 acaataaaaa actgaagaat accattttag gattcctcag tttttcatca cctattttac
     2701 ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa ccttttaacc
     2761 cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg gttggtccgc
     2821 ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg atttgatgga
     2881 tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt ttgatcatct
     2941 gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga tgttggatgg
     3001 cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt ggtttgacca
     3061 acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct ttgccaccga
     3121 caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc aaaccgattt
     3181 ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct cttagagcag
     3241 ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc ttcttaacaa
     3301 agatcttgac accggttttg atattgtatt tattttttag caataaagca gcttgataaa
     3361 cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg attttgtctt
     3421 ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc accatttcca
     3481 actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt atcttggctg
     3541 gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc gcaagtattc
     3601 tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt ttgtcatcgt
     3661 ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt gattcttata
     3721 attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt cctatcagac
     3781 tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca aaataacgtc
     3841 cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag gaatagaaaa
     3901 aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa aaatctgttt
     3961 ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc attttctcgt
     4021 atacaataaa cagattggct aacagatagt cagctaattc ctcagattct ttaggtaaga
     4081 cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg atgaagccaa
     4141 cgactttttg atctttacgg acaataccga caccgctaga taatacatat gatttatcaa
     4201 atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt gaaacttcat
     4261 ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg tatccttctt
     4321 tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct gttgctagat
     4381 caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga agcatcacta
     4441 cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct gcttgtttca
     4501 taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct cctaaaacaa
     4561 agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg tcttcttggt
     4621 aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg cctccgtatg
     4681 tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc gtagtatctt
     4741 cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa ccaataagcc
     4801 ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt attcgaccga
     4861 ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa aagccgaaca
     4921 agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga taaaattctt
     4981 ttctggataa ccacacagct aacagaatca agacatatac caatatcaac tgccaagata
     5041 acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta ggcagaaatg
     5101 cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag ccaaccaata
     5161 gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga aatagccgac
     5221 tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc gcaaaataga
     5281 cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga atgttatcta
     5341 aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc gtcataaaag
     5401 gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca tcttgccgaa
     5461 cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt ccacctggga
     5521 ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc gaaagcatcg
     5581 aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct cccgtccatt
     5641 gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg aagaacaatg
     5701 tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta ccgccgaaaa
     5761 gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca tcaataaatg
     5821 taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt gatacagtgg
     5881 ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga cttgctccta
     5941 ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca gcaagaagaa
     6001 actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc gtcacccaat
     6061 catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc aggatcgtca
     6121 tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca ttagcaatat
     6181 ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt tttaaaatca
     6241 gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca ttttcccatt
     6301 cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt aatttttcca
     6361 ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa tgctaaagga
     6421 gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc tattattgat
     6481 ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag tggacatttt
     6541 gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag taatcaggaa
     6601 gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat tgaaaaagta
     6661 ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc ttcaggaaaa
     6721 tcattaacag atttagaaac agcgcttgaa gatcctcagg cctccatcgt acgtgtgatg
     6781 ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa tgaagctgcc
     6841 acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag cgtctatcct
     6901 ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc cgcattcgtt
     6961 tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc caaacagaca
     7021 gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct ttcaagtgat
     7081 aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac tgttgcaggt
     7141 gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat caatacgacg
     7201 atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt tttcgtgcct
     7261 tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt tttattattt
     7321 agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc caggataatc
     7381 cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt ctaaatccga
     7441 gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga
     7501 ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac ggatgtagtt
     7561 ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg ctgcagctac
     7621 aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga tcgcgttgat
     7681 tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa cgtgagatcc
     7741 ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac gttttgctaa
     7801 ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa cctcgaccga
     7861 atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga tgatcttgat
     7921 tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg
     7981 aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac gcgctggatg
     8041 gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc ttcggattgg
     8101 atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat cgataccagt
     8161 cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac ctttcatttc
     8221 atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg
     8281 ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt tggcacgctg
     8341 ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag tcgatttcat
     8401 gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt ctttttgttg
     8461 atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct gttttgaact
     8521 aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt catctttacg
     8581 attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt ctaacttact
     8641 ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct tagcttctaa
     8701 caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga ttccagcagc
     8761 tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca taaaccctaa
     8821 accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca taaaatccac
     8881 ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa ttattctaaa
     8941 ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact ttattctaat
     9001 gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta cttattcatt
     9061 aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt taaattgaga
     9121 aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta tgtacccact
     9181 cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag
     9241 tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc
     9301 aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct
     9361 gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta
     9421 gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc accgctttta
     9481 tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa
     9541 ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact
     9601 ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt
     9661 acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga
     9721 ttcccaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt
     9781 gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac
     9841 ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact
     9901 gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga
     9961 ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt
    10021 tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt
    10081 ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca
    10141 tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca
    10201 agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata
    10261 gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga
    10321 tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc
    10381 ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat
    10441 cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg
    10501 atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa
    10561 atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg
    10621 gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc
    10681 acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa
    10741 ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca
    10801 gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca
    10861 gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg
    10921 tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct
    10981 tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt
    11041 ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg
    11101 accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca
    11161 gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca
    11221 ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa
    11281 gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga
    11341 ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct
    11401 tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca
    11461 tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga
    11521 gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg
    11581 atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct
    11641 agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata
    11701 tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag ctgtgacaaa
    11761 aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa cctcggtcat
    11821 aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct tgtgactcag
    11881 agttagacgg caggctcaca acctctccac taaacgatac ttacattgat cctttgaaca
    11941 caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc agacaagcat
    12001 aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta ttgataaaca
    12061 gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gctgcagcca
    12121 tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat tcacgacaga
    12181 caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg ataaaagcag
    12241 tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc
    12301 ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa gcaaaaataa
    12361 ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat tgtccccaat
    12421 ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta
    12481 atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt gactatcagc
    12541 gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga gtatttttat
    12601 tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt cacgatcctt
    12661 cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca ttctgcgtat
    12721 tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt aactggtacg
    12781 gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat ggtcgcattt
    12841 gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt cccttgataa
    12901 acataggcat tgtctactag cacacctaac catacacgat tgacagcagt aaattcaagg
    12961 gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga atcttctcct
    13021 gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac tgtagaagac
    13081 tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc ttctacttta
    13141 agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca agtcatatag
    13201 cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg aagacttgtc
    13261 caaaagcgac ttggatgcga ttcttcctga tgcattgctt ttctagaagt acttactgtt
    13321 tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg
    13381 actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa tgcccgcacg
    13441 taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt
    13501 tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag gcgtgcttgc
    13561 cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt ctgtttgatt
    13621 ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg acaaaagtca
    13681 tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc ggtcacaagt
    13741 cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg actcggagtt
    13801 aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta
    13861 gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta atactcaggt
    13921 caaaacgctg atttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
    13981 ataagtccaa ccaagttaaa aacattaaga agctgttttt acttggctgt tcttattact
    14041 tgatgcaggg cagctttttc acaacctctt ggtacacggt gttccagaag tagcttcttc
    14101 ggcaataagc cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    14161 tcttgaagct gaccacttct gtcacaacct ctttgattac ttcgtttgat tttattttaa
    14221 tataagttga ttaaaagaga gcatgatttg ttttattttc tcactaattt ttccaacaca
    14281 actaagaata ccataaaagc tgattttgga ggaggttcct actacaagtc taaaacgaaa
    14341 tttaagactt ttcctcataa aatactggtt tattttggtc tcatgtaaaa ctcgctgaag
    14401 gcctcttcac ttataaatgc ttccccagct gccagtacgt ctttcatttg gatcgaatcg
    14461 atgatttcag gaagatcaaa taaagttcga tcgccaaata aactttgcgt aaattgattg
    14521 gcaatatatt cgatcgaatt taatgattgg aaatattgtc caagcatttt cttcttcagc
    14581 aaatcgagat ttgtttctga cacttccgga tcatcagcga atccaagaat gatttctttg
    14641 actttttctg ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat
    14701 tcacgatcca agctgaattc aaaaccaaag ctgtcatcga taattccttg attgtacatc
    14761 gctagatagt ttcgagaagt attgcctaaa atcatttgga acaacagatt gattgctgtt
    14821 ttatagcgta acagttctgt tccttcttgc ggcaatgtgt ctaatccttt aataccaagt
    14881 acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg
    14941 ttttcaggga aataacgtac aatttcttgc tttggaggga aattttttgc ttcttggttt
    15001 tcccgaataa gtttcatcaa tttctcaggc tccatcttac caactacaaa taaaaccata
    15061 ttgcttggct gataaaacgt ccgatagcac gtatacaagt cttgagctgt gattttgtcg
    15121 atactttcaa ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata caagttattt
    15181 agtatgccaa aaaacatccg ccagttaggg tcgtcttcat acatttggat ttcctgaccg
    15241 atgattcctt tttccttgtt tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg
    15301 atcagagtcg tcagattctt ttctacctga tccgtcgtag aaaacagata gctggttttc
    15361 gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc caaataattg aaagacatca
    15421 ccatcttctt tttcaaacaa cttatgttct aaaaaatggg caattccatc tggaactttc
    15481 accttttctt tttcaccata gggaataaat tcgttatcga tcgaaccata attcgtactg
    15541 aacaaaccat atgtcttgtg atagtcattt ttaggcaaca aatacacggt caaaccgttt
    15601 ggcagtactt catgataaag tgtttcattg atttgctcat attctgtttt attcatttgc
    15661 atttcctcct gctaaaaaga agattgcctg caattcgatt tgctcagcta cttgttgtat
    15721 ctctttgatc gttacgctct ccatacgttt caaccattct tcatcagata attttgtttc
    15781 cggcagccag ctgtccaaaa aagaagcttc gattgccgct tgcggagagt ctagagaaag
    15841 cagatattgg tttctcagca ttgcttttgt ctgagacagt tccaactctg ttatttcacc
    15901 atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt acttgatttc ggttcttttc
    15961 atcgataccg gtttggacac tcatgaatcc tcggaacgta tccacgctac tagaggcata
    16021 ataagctaga ctttctttct ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc
    16081 aaataaaccg ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat acacatttgt
    16141 ctgataagct aggttcagct ttgcttgaac gattggctca cgtacttgtc gttccttcac
    16201 gatattcact tgaggttggg tataaaacag atctggataa actgtttccc gcgactcaaa
    16261 tggtaattgc ccaactgctt cagctgcttt gtccggatca acatcgccga caacaaaaat
    16321 gtcgatttga tcatttgcca tcatttcttg atagtaggac gctagcgaac tagcagtgat
    16381 cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa
    16441 ataaagttct tgcaaggcat aggaagcaaa agtttgttta tcttccttca aactttccaa
    16501 atatagacgc agattgttct tttccaagtc aaacgtcaac tgatcaaact gatgattttt
    16561 aatatttgga taaaataaaa cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg
    16621 atcattgaca tattcaccat tcacaaaact gatccctgcg tttagccaat gtaaatttcc
    16681 tttttttcta actgataatc caaagcttgc gccataaagc tctgcaagtt gcgcacttaa
    16741 ctttgtctga tccgaataat tcaaactatt tgtttccaac atgctgctga gaagtgaacg
    16801 tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt
    16861 gtattgtttt gttggtaaga catgaagatt cacgccttct gccaattgat acaccatatt
    16921 tatatgctcc ttttcacagt tgattgcccg cttactcttt taatgggtca acctgccttt
    16981 tttacttatt cctctgcttg aaatttataa gttcaggcaa ttcgccgttt tttctaaatg
    17041 atgttacact ttatagtata gcatagatgt tttttatcaa aagaaacaga cggctaaaag
    17101 cgtatattgt ttttttattt tttatttttc gatatactaa agacaaataa aagaagggag
    17161 ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg
    17221 ctgtcggcgt tgttattggt agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct
    17281 tgatcacacc actaataggt tggtttgtcg caatgatcac aggtacttct gatttagaag
    17341 gagcactttc aattcttgac tggtcgccga caaaaggcgt cacatttgcg tttggtaacg
    17401 ttattagtgc tatcattaca tttctgatca ctggttttgt cttattctta gttgtcaaag
    17461 cggcaaaccg tgcgaagaat ttacgctcca aagaagagca agtggaagtg gaagaaactc
    17521 ctactgcaga agattatctt agtgacatac gcgatcttct tgccaaacaa gtggcagaag
    17581 acaatacaca tccagtagag caaacaaatc tttccgatac ttctaaataa taaagaatct
    17641 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    17701 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    17761 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    17821 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    17881 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    17941 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    18001 agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    18061 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    18121 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    18181 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    18241 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    18301 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    18361 aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    18421 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    18481 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca taaaaaaaaa
    18541 agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta ttcctcatta
    18601 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
    18661 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
    18721 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
    18781 aataaaagat gctgaattgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
    18841 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
    18901 gcgttctgct tcctcaacag agaatagatt tgattgctta taacgctttt ataataactt
    18961 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
    19021 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
    19081 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
    19141 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
    19201 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
    19261 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
    19321 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
    19381 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
    19441 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
    19501 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
    19561 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
    19621 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
    19681 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
    19741 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
    19801 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
    19861 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
    19921 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
    19981 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
    20041 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
    20101 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
    20161 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
    20221 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
    20281 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
    20341 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
    20401 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
    20461 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
    20521 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
    20581 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
    20641 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
    20701 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
    20761 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
    20821 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
    20881 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
    20941 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
    21001 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
    21061 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
    21121 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
    21181 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
    21241 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
    21301 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
    21361 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
    21421 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
    21481 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
    21541 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
    21601 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
    21661 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
    21721 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
    21781 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
    21841 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
    21901 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
    21961 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
    22021 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
    22081 catatgatcc atgactcttg gatgcagtgt agcaggttcg acattcaacg ctttcgtatc
    22141 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
    22201 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
    22261 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
    22321 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
    22381 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
    22441 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
    22501 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
    22561 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
    22621 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
    22681 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
    22741 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
    22801 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
    22861 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
    22921 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
    22981 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
    23041 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
    23101 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
    23161 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
    23221 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
    23281 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
    23341 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
    23401 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
    23461 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
    23521 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
    23581 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
    23641 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
    23701 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
    23761 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
    23821 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
    23881 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
    23941 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
    24001 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
    24061 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
    24121 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
    24181 cgtttttgga tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
    24241 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
    24301 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
    24361 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
    24421 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
    24481 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
    24541 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
    24601 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
    24661 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
    24721 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
    24781 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
    24841 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
    24901 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
    24961 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
    25021 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
    25081 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
    25141 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
    25201 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
    25261 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
    25321 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
    25381 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
    25441 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
    25501 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
    25561 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
    25621 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
    25681 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
    25741 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
    25801 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
    25861 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
    25921 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
    25981 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
    26041 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
    26101 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
    26161 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
    26221 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
    26281 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
    26341 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
    26401 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
    26461 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
    26521 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
    26581 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
    26641 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
    26701 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
    26761 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
    26821 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cggttttgag
    26881 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
    26941 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
    27001 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
    27061 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
    27121 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
    27181 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
    27241 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
    27301 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
    27361 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
    27421 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
    27481 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
    27541 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
    27601 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
    27661 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
    27721 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
    27781 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
    27841 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
    27901 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
    27961 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
    28021 atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
    28081 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
    28141 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
    28201 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
    28261 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
    28321 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
    28381 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
    28441 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
    28501 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
    28561 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    28621 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    28681 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    28741 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    28801 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    28861 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    28921 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    28981 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    29041 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    29101 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    29161 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    29221 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    29281 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    29341 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    29401 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg atcggcatca
    29461 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    29521 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    29581 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    29641 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    29701 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    29761 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    29821 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    29881 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    29941 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    30001 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    30061 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    30121 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    30181 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    30241 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    30301 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    30361 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    30421 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    30481 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    30541 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    30601 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    30661 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    30721 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    30781 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    30841 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    30901 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    30961 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    31021 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    31081 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    31141 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    31201 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    31261 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    31321 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    31381 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    31441 cagtctcaca acctcttggt aaacggcgtt caatcagctg accctcggta ttagctcaaa
    31501 aaacggtaaa atatgagaag ctatttttcc gttttgtttc tctaatactt aggataaaac
    31561 gctgatttta caacctctgt taaacggtgt tcaatcagct gtccttcggt attagctcaa
    31621 aaaacggcaa aatatgagga actatttttc cgtttgtttc tctaatactc aggacaaaac
    31681 gctgatttta caacctcctg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    31741 caaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag
    31801 gacagctttt tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg
    31861 gtgataaatg atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata
    31921 gctttcacca atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa
    31981 aaagcggaca aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat
    32041 agcaatcgtc gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct
    32101 gatcggtaca aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa
    32161 taagacaaat gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc
    32221 aacgaaaatc gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc
    32281 taattcagaa tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa
    32341 attatataac aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac
    32401 accttcccaa aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag
    32461 ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc
    32521 actgcccaag accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc
    32581 atacatcatg attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc
    32641 agtagagaca aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc
    32701 gatattccca gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat
    32761 caatgttttt ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc
    32821 agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg
    32881 tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca
    32941 ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg
    33001 tatatctatt tgattctcca atatttccat ccctttctct ctattagcat catacatgat
    33061 ttattttaca tgtcaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag
    33121 aaaataagac gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc
    33181 ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag
    33241 cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg
    33301 ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg
    33361 ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc
    33421 tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca
    33481 ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct
    33541 cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt
    33601 gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt
    33661 aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa
    33721 gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg
    33781 atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt
    33841 tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa
    33901 agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca
    33961 ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa
    34021 gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc
    34081 ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg
    34141 aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat
    34201 aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa
    34261 cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc
    34321 tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt
    34381 tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc
    34441 atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat
    34501 cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt
    34561 aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt
    34621 cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga
    34681 aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc
    34741 cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt
    34801 gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt
    34861 tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa
    34921 ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg
    34981 aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct
    35041 ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga
    35101 agacaaccga tccagtctga tttgttgctt tttcttttta ctggacactt tttccaactt
    35161 ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat
    35221 cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact
    35281 ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag
    35341 ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag
    35401 ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag
    35461 catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc
    35521 gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa
    35581 atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc
    35641 tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga
    35701 tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga
    35761 atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc
    35821 taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa
    35881 aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga
    35941 aataactatg actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga
    36001 aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta
    36061 gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt
    36121 ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa
    36181 accttttatt cgaacatagt gatcttttct ggtgctaaac gaacagactc gtaaccttct
    36241 tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt atccaaacca
    36301 aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga
    36361 tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg
    36421 ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct
    36481 ctgacttctt ttggcatctt ccctgattcg taagcttggt tgtagatttc ttcgccatat
    36541 tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc
    36601 gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca
    36661 aagcgccatg gtttcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc
    36721 agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca
    36781 gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt
    36841 tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc
    36901 tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg
    36961 cttgattttc ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg
    37021 atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc
    37081 tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt
    37141 cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt caaagatctg
    37201 gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga
    37261 cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata
    37321 aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag
    37381 tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc
    37441 taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg cagtagataa
    37501 ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa
    37561 attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt
    37621 ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa
    37681 acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac
    37741 tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag
    37801 ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt
    37861 aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca
    37921 accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact
    37981 gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc
    38041 ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca atgtatataa
    38101 atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt
    38161 atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg
    38221 cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct
    38281 tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt
    38341 tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt
    38401 aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc cattatagct
    38461 tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc
    38521 actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa
    38581 actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg
    38641 agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg
    38701 tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt
    38761 ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg
    38821 ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac tttctgtatc
    38881 gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc
    38941 ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa
    39001 taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag
    39061 tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt
    39121 aataaatttg ctttttctgc ataaaaagac gtaaatgaag aatattcttc tccattcacg
    39181 ccttaaattg atggtcgttt gaataactat ttaacaagta attctctctg gatattatcc
    39241 gttatggatt atacttcctg tgtcgtcgga cggataatca tgttgctgac agacatccgg
    39301 tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcagatgt taatccccat
    39361 tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat
    39421 tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct
    39481 tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa
    39541 tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt
    39601 ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca
    39661 agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct
    39721 gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct
    39781 tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa
    39841 gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct
    39901 ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca
    39961 atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct
    40021 tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg
    40081 attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca
    40141 ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct
    40201 ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt
    40261 ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct
    40321 tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc
    40381 cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca
    40441 tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac
    40501 ccagtattca gcgctgctaa aggctcttcg ttttccgctt ggatctgatg gatcacttca
    40561 aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc
    40621 attttgctcc ctccttttat ccttacttta taacggacgg cttatgatgt ctaatactta
    40681 taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg
    40741 agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat
    40801 atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa
    40861 gatttagaag aagaacttgg tgtgacactt ttcactcgag gctcccgaga gatacagttg
    40921 acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa
    40981 acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc
    41041 gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct
    41101 gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc
    41161 ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt
    41221 cgactgccag cccaagatcg ttggggactt ttgatacatc ggaatcatcc tttagcaaag
    41281 caaacagtca tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg
    41341 tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc
    41401 ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca
    41461 ttagctctag atgggatcat caatacttat gggacggaaa aagtattcat cccactcgat
    41521 ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct
    41581 gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc
    41641 gctttcagct ttttaacaac gaataagtta agcaaaagat ccaatgatta aataacataa
    41701 gtttcttaat ttgttccttc cttttatttc tgataattcc agtaaattac tataattata
    41761 aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg
    41821 tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg
    41881 aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc
    41941 aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat
    42001 tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa
    42061 aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag
    42121 gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag
    42181 aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga
    42241 tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc
    42301 gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat
    42361 gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat
    42421 atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc
    42481 gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata
    42541 tccattcaga accattttca ctgaattatt caagactggt gaatcattta cgactgcttc
    42601 tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca
    42661 aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag
    42721 aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt
    42781 taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc
    42841 atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc
    42901 tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt
    42961 tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt
    43021 agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt
    43081 tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc
    43141 agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg
    43201 attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc
    43261 tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag
    43321 tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt
    43381 cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat
    43441 ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa
    43501 tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc
    43561 tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa
    43621 tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ttagcagcag cttttgcttc
    43681 attctttact ggtgtaacag aaccacttga attctcattt atgttcgttg cttggccatt
    43741 atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg
    43801 gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc
    43861 gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta
    43921 ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc
    43981 agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc
    44041 aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac
    44101 tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc
    44161 cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac
    44221 agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg caacacgacc
    44281 tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga
    44341 tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt
    44401 cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc
    44461 tccaaccgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac
    44521 agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga
    44581 accattcact ttatatgtaa aagaaggtca aaaagtcgca agaggtcaat tgattgcttt
    44641 agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac
    44701 aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga
    44761 taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg
    44821 ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg
    44881 gcgttctcct gccaactccg cggtaataag tccagccaaa cgtaacatat gaagagctat
    44941 tttttatttg actcttctta ttacttgatg cagaacagct tttttacaac ctctgataaa
    45001 cggtgttcaa aaaccagttt atcggtatga agtcaaattt atgcaaaaat gtggaaagcc
    45061 attttcacaa aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata
    45121 cattattcct gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc
    45181 aatacttttt ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg
    45241 atcgctaaaa acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca
    45301 aacgctgcat cacttgcttc aataatttct aactttttac ctgtcgcacg ctctaatgcc
    45361 ttggctaatt cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca
    45421 ctttctacta gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt
    45481 tttccatttc ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt
    45541 aattcattct ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta
    45601 atcatttttt cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct
    45661 gcaaaacttg tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc
    45721 ttatgttcgg cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct
    45781 ttcaacgctt ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca
    45841 gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt
    45901 ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt
    45961 actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta
    46021 catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat
    46081 gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc
    46141 ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat
    46201 cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat
    46261 ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt
    46321 actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac
    46381 aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg
    46441 gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa
    46501 aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt
    46561 ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta
    46621 ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag
    46681 cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc
    46741 tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa
    46801 gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct
    46861 cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag
    46921 gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt
    46981 aattcttcag gcgattctgc taaagcgatt acaacaagat acgttccttt tgggaatggg
    47041 atgattcggg tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt
    47101 ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat
    47161 gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa
    47221 ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct
    47281 ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca
    47341 tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta
    47401 ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa
    47461 tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaactcata atctgaagaa
    47521 ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct
    47581 ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac
    47641 ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag
    47701 cgggctccat caaatccata agaaacgttg attacttgat ttggatataa atctcctaac
    47761 ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga
    47821 ttgactcctt caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct
    47881 ttcttttcga tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag
    47941 cgtggaaaag atttttgtat gccgtatctg ctgatttctg aaggcagata tcctgaccaa
    48001 tcacgaaaag ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct
    48061 gtcactcttg atccatctag gagatcgcga ttagccaaag ccaatctacg gtttttgata
    48121 tagtccatta gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag
    48181 attcctgtat tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg
    48241 taatcgatca attggttcag atctttaagc atatttatct tcctccttgc ttgccttttt
    48301 atcatattat caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta
    48361 gaaaatgttg tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt
    48421 attatctgcc tatctatgtt ttacctttag aaaaataaat tttggggaag acacattcta
    48481 agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg
    48541 tacaaagcaa attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga
    48601 tacatcatac ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt
    48661 tttgtcagat aaataaaaaa caaaaaaagt tccatcttta aaacacgcta ttcttttact
    48721 ttttctcaca ttctctaaga aaagaaaagg agaatgaatt tttgacaatt cg
//
LOCUS       DAZUMV010000007        45536 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000007 DAZUMV010000000
VERSION     DAZUMV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.007"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8FK_000381"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8FK_000381"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS5096021.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8FK_000382"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8FK_000382"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS5096022.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8FK_000383"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8FK_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS5096023.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8FK_000384"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8FK_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS5096024.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8FK_000385"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8FK_000385"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS5096025.1"
                     /translation="MLEKDIERILISQEEIQVRCKELAKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8FK_000386"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8FK_000386"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS5096026.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8FK_000387"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8FK_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS5096027.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8FK_000388"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8FK_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS5096028.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8FK_000389"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8FK_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS5096029.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8FK_000390"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8FK_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5096030.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLGALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8FK_000391"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8FK_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS5096031.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8FK_000392"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8FK_000392"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS5096032.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG8FK_000393"
     CDS             15402..16376
                     /locus_tag="ACG8FK_000393"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5096033.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG8FK_000394"
     CDS             16546..17946
                     /locus_tag="ACG8FK_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS5096034.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG8FK_000395"
     CDS             complement(18024..18746)
                     /locus_tag="ACG8FK_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5096035.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG8FK_000396"
     CDS             complement(18825..19331)
                     /locus_tag="ACG8FK_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5096036.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG8FK_000397"
     CDS             19454..21253
                     /locus_tag="ACG8FK_000397"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS5096037.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACG8FK_000398"
     CDS             complement(21321..22895)
                     /locus_tag="ACG8FK_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS5096038.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLHQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8FK_000399"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8FK_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS5096039.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8FK_000400"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8FK_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS5096040.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACG8FK_000401"
     CDS             complement(24672..25310)
                     /locus_tag="ACG8FK_000401"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS5096041.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8FK_000402"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8FK_000402"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS5096042.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8FK_000403"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8FK_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS5096043.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8FK_000404"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8FK_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS5096044.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACG8FK_000405"
     CDS             complement(29104..29844)
                     /locus_tag="ACG8FK_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS5096045.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8FK_000406"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8FK_000406"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS5096046.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8FK_000407"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8FK_000407"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS5096047.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8FK_000408"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8FK_000408"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5096048.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8FK_000409"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8FK_000409"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS5096049.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8FK_000410"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8FK_000410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5096050.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8FK_000411"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8FK_000411"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5096051.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="ACG8FK_000412"
     CDS             complement(36724..37008)
                     /locus_tag="ACG8FK_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096052.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="ACG8FK_000413"
     CDS             complement(37026..37916)
                     /locus_tag="ACG8FK_000413"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS5096053.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="ACG8FK_000414"
     CDS             complement(37936..38154)
                     /locus_tag="ACG8FK_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS5096054.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="ACG8FK_000415"
     CDS             complement(38124..39824)
                     /locus_tag="ACG8FK_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS5096055.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="ACG8FK_000416"
     CDS             40091..40864
                     /locus_tag="ACG8FK_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096056.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ACG8FK_000417"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ACG8FK_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS5096057.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="ACG8FK_000418"
     CDS             complement(41688..42659)
                     /locus_tag="ACG8FK_000418"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5096058.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ACG8FK_000419"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ACG8FK_000419"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS5096059.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEAFFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ACG8FK_000420"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ACG8FK_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS5096060.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
BASE COUNT        12408 a         9872 c         8242 g        15014 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttggct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcaccta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgatgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaaaaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaa
//
LOCUS       DAZUMV010000008        44045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000008 DAZUMV010000000
VERSION     DAZUMV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.008"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..389)
                     /locus_tag="ACG8FK_000421"
     CDS             complement(<1..389)
                     /locus_tag="ACG8FK_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096061.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFIT"
     gene            complement(686..3067)
                     /locus_tag="ACG8FK_000422"
     CDS             complement(686..3067)
                     /locus_tag="ACG8FK_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5096062.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3057..3704)
                     /gene="recU"
                     /locus_tag="ACG8FK_000423"
     CDS             complement(3057..3704)
                     /gene="recU"
                     /locus_tag="ACG8FK_000423"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS5096063.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3768..4307
                     /locus_tag="ACG8FK_000424"
     CDS             3768..4307
                     /locus_tag="ACG8FK_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS5096064.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4397..4816
                     /gene="gpsB"
                     /locus_tag="ACG8FK_000425"
     CDS             4397..4816
                     /gene="gpsB"
                     /locus_tag="ACG8FK_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS5096065.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4838..5205
                     /gene="rnpB"
                     /locus_tag="ACG8FK_000426"
     ncRNA           4838..5205
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8FK_000426"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5414..6580
                     /locus_tag="ACG8FK_000427"
     CDS             5414..6580
                     /locus_tag="ACG8FK_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS5096066.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6626..8122
                     /locus_tag="ACG8FK_000428"
     CDS             6626..8122
                     /locus_tag="ACG8FK_000428"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS5096067.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8203..8886
                     /locus_tag="ACG8FK_000429"
     CDS             8203..8886
                     /locus_tag="ACG8FK_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5096068.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9531..10721
                     /locus_tag="ACG8FK_000430"
     CDS             9531..10721
                     /locus_tag="ACG8FK_000430"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS5096069.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10823..11776
                     /locus_tag="ACG8FK_000431"
     CDS             10823..11776
                     /locus_tag="ACG8FK_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS5096070.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11845..13194
                     /locus_tag="ACG8FK_000432"
     CDS             11845..13194
                     /locus_tag="ACG8FK_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5096071.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13377..13700)
                     /locus_tag="ACG8FK_000433"
     CDS             complement(13377..13700)
                     /locus_tag="ACG8FK_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096072.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13829..14992
                     /locus_tag="ACG8FK_000434"
     CDS             13829..14992
                     /locus_tag="ACG8FK_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5096073.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            15062..16411
                     /locus_tag="ACG8FK_000435"
     CDS             15062..16411
                     /locus_tag="ACG8FK_000435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5096074.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16456..16886)
                     /locus_tag="ACG8FK_000436"
                     /pseudo
     CDS             complement(16456..16886)
                     /locus_tag="ACG8FK_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            17274..19916
                     /gene="alaS"
                     /locus_tag="ACG8FK_000437"
     CDS             17274..19916
                     /gene="alaS"
                     /locus_tag="ACG8FK_000437"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS5096075.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            20116..20712
                     /locus_tag="ACG8FK_000438"
     CDS             20116..20712
                     /locus_tag="ACG8FK_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5096076.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20834..21982
                     /locus_tag="ACG8FK_000439"
     CDS             20834..21982
                     /locus_tag="ACG8FK_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5096077.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(22020..22433)
                     /locus_tag="ACG8FK_000440"
     CDS             complement(22020..22433)
                     /locus_tag="ACG8FK_000440"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS5096078.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22512..22943
                     /locus_tag="ACG8FK_000441"
     CDS             22512..22943
                     /locus_tag="ACG8FK_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS5096079.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(23042..23275)
                     /locus_tag="ACG8FK_000442"
     CDS             complement(23042..23275)
                     /locus_tag="ACG8FK_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5096080.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23458..25128
                     /locus_tag="ACG8FK_000443"
     CDS             23458..25128
                     /locus_tag="ACG8FK_000443"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS5096081.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            25275..25661
                     /locus_tag="ACG8FK_000444"
     CDS             25275..25661
                     /locus_tag="ACG8FK_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096082.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25648..26346
                     /locus_tag="ACG8FK_000445"
     CDS             25648..26346
                     /locus_tag="ACG8FK_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS5096083.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26351..26815
                     /gene="lspA"
                     /locus_tag="ACG8FK_000446"
     CDS             26351..26815
                     /gene="lspA"
                     /locus_tag="ACG8FK_000446"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS5096084.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26812..27723
                     /locus_tag="ACG8FK_000447"
     CDS             26812..27723
                     /locus_tag="ACG8FK_000447"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5096085.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27958..28497
                     /gene="pyrR"
                     /locus_tag="ACG8FK_000448"
     CDS             27958..28497
                     /gene="pyrR"
                     /locus_tag="ACG8FK_000448"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS5096086.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28511..29794
                     /locus_tag="ACG8FK_000449"
     CDS             28511..29794
                     /locus_tag="ACG8FK_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS5096087.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29807..30733
                     /locus_tag="ACG8FK_000450"
     CDS             29807..30733
                     /locus_tag="ACG8FK_000450"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS5096088.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30752..32032
                     /locus_tag="ACG8FK_000451"
     CDS             30752..32032
                     /locus_tag="ACG8FK_000451"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS5096089.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            32037..33113
                     /locus_tag="ACG8FK_000452"
     CDS             32037..33113
                     /locus_tag="ACG8FK_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS5096090.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            33118..36303
                     /gene="carB"
                     /locus_tag="ACG8FK_000453"
     CDS             33118..36303
                     /gene="carB"
                     /locus_tag="ACG8FK_000453"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS5096091.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36357..37133
                     /locus_tag="ACG8FK_000454"
     CDS             36357..37133
                     /locus_tag="ACG8FK_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS5096092.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            37130..38062
                     /locus_tag="ACG8FK_000455"
     CDS             37130..38062
                     /locus_tag="ACG8FK_000455"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS5096093.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            38059..38775
                     /gene="pyrF"
                     /locus_tag="ACG8FK_000456"
     CDS             38059..38775
                     /gene="pyrF"
                     /locus_tag="ACG8FK_000456"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS5096094.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38782..39411
                     /gene="pyrE"
                     /locus_tag="ACG8FK_000457"
     CDS             38782..39411
                     /gene="pyrE"
                     /locus_tag="ACG8FK_000457"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS5096095.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39399..39713
                     /locus_tag="ACG8FK_000458"
     CDS             39399..39713
                     /locus_tag="ACG8FK_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096096.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39751..40392
                     /locus_tag="ACG8FK_000459"
     CDS             39751..40392
                     /locus_tag="ACG8FK_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS5096097.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40435..41127
                     /locus_tag="ACG8FK_000460"
     CDS             40435..41127
                     /locus_tag="ACG8FK_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS5096098.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            41124..42026
                     /locus_tag="ACG8FK_000461"
     CDS             41124..42026
                     /locus_tag="ACG8FK_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5096099.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(42184..43890)
                     /gene="efbA"
                     /locus_tag="ACG8FK_000462"
     CDS             complement(42184..43890)
                     /gene="efbA"
                     /locus_tag="ACG8FK_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS5096100.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        14154 a         7736 c         9542 g        12613 t
ORIGIN      
        1 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
       61 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
      121 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
      181 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
      241 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
      301 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
      361 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
      421 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
      481 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
      541 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
      601 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
      661 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
      721 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
      781 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
      841 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
      901 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
      961 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     1021 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     1081 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     1141 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     1201 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     1261 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     1321 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     1381 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
     1441 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
     1501 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
     1561 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
     1621 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
     1681 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
     1741 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
     1801 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
     1861 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
     1921 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
     1981 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
     2041 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
     2101 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
     2161 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
     2221 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
     2281 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
     2341 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
     2401 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
     2461 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
     2521 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
     2581 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
     2641 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
     2701 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
     2761 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
     2821 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
     2881 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
     2941 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
     3001 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
     3061 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
     3121 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
     3181 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
     3241 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
     3301 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
     3361 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
     3421 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
     3481 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
     3541 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
     3601 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
     3661 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
     3721 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
     3781 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
     3841 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
     3901 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
     3961 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
     4021 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
     4081 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
     4141 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
     4201 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
     4261 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
     4321 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
     4381 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
     4441 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
     4501 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
     4561 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
     4621 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
     4681 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
     4741 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
     4801 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
     4861 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
     4921 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
     4981 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
     5041 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
     5101 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
     5161 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
     5221 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
     5281 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
     5341 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
     5401 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
     5461 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
     5521 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
     5581 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
     5641 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
     5701 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
     5761 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
     5821 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
     5881 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
     5941 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
     6001 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
     6061 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
     6121 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
     6181 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
     6241 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
     6301 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
     6361 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
     6421 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
     6481 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
     6541 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
     6601 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
     6661 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
     6721 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
     6781 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
     6841 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
     6901 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
     6961 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
     7021 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
     7081 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
     7141 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
     7201 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
     7261 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
     7321 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
     7381 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
     7441 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
     7501 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
     7561 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
     7621 ttatcgttta ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
     7681 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
     7741 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
     7801 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
     7861 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
     7921 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
     7981 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
     8041 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
     8101 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
     8161 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
     8221 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
     8281 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
     8341 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
     8401 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
     8461 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
     8521 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
     8581 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
     8641 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
     8701 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
     8761 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
     8821 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
     8881 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
     8941 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
     9001 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
     9061 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
     9121 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
     9181 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
     9241 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
     9301 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
     9361 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
     9421 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
     9481 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
     9541 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
     9601 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
     9661 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
     9721 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
     9781 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
     9841 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
     9901 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
     9961 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    10021 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    10081 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    10141 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    10201 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    10261 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    10321 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    10381 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    10441 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    10501 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    10561 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    10621 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    10681 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    10741 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    10801 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    10861 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    10921 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    10981 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    11041 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    11101 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    11161 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    11221 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    11281 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    11341 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    11401 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    11461 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    11521 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    11581 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    11641 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    11701 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    11761 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    11821 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    11881 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    11941 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    12001 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    12061 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    12121 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    12181 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    12241 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    12301 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    12361 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    12421 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    12481 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    12541 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    12601 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    12661 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    12721 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    12781 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    12841 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    12901 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    12961 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    13021 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    13081 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    13141 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    13201 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    13261 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    13321 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    13381 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    13441 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    13501 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    13561 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    13621 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    13681 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    13741 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    13801 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    13861 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    13921 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    13981 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    14041 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    14101 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    14161 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    14221 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    14281 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    14341 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    14401 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    14461 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    14521 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    14581 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    14641 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    14701 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    14761 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    14821 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    14881 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    14941 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    15001 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    15061 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    15121 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    15181 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    15241 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    15301 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    15361 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    15421 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    15481 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    15541 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    15601 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    15661 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    15721 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    15781 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    15841 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    15901 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    15961 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    16021 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    16081 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    16141 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    16201 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    16261 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    16321 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    16381 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    16441 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    16501 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    16561 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    16621 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    16681 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    16741 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    16801 aatcccttgt tctaatacaa aaattgatat ctcaaaaagc agctagctgc gcgtatccat
    16861 tcatccgctc caactatttt tttcatcata ttcctccttg tcatgaatag cagagatgta
    16921 ttgattcctt tattcttatc gtaccaaaaa tcgtggagta tgaccaaaag gaaatgaaaa
    16981 taaaaattga gatattgaca gtgatcaata gaatgcgtat actaattaag tacgaaaagg
    17041 atatgatttg ttttttaaga ctcacagaaa attctttctt ggctgaaaaa agaatagtca
    17101 ctaatcaaat tgctcccttt ttctattttg gtagaaatac cgacgcagct cagcgttatc
    17161 gagtttaaga ggtaatgatt acacatcatt gcaatcaagg tggtaccgcg agttttcgtc
    17221 cttggctgtg atggtgtttt tttgtattta atttatagaa agcaggtaat gacatgaaac
    17281 aattaagtag tgcacaagtt cgacaaatgt ttcttgattt cttccaatct aaaggacata
    17341 cgattgaacc tagtgcgtct ttgatccccg tgaacgatcc aaccttattg tggatcaact
    17401 ctggtgttgc aacattgaaa aaatattttg atggttcagt tgtgccagaa aatccaagaa
    17461 tcacaaatgc ccaaaaatct attcgaacaa atgatatcga aaatgtcggt aaaacagcac
    17521 ggcaccatac gatgtttgag atgctgggta atttttcgat tggtgactat ttcaaaaatg
    17581 aagccatcca ctgggcatgg gaatttttga ctgatccaaa atggatggca ttcgatccag
    17641 aaaaacttta tgtaacagtt tatccaaaag atacagaagc aaaacgcatc tggcatgaag
    17701 aagttggcct gccactagat cacattattg atgtagaaga caacttctgg gatatcggtg
    17761 caggcccatg tggtccggat acggaaattt tctatgaccg tggagaagct tataacgatt
    17821 tagcagaaga cgatcctgaa aactatccag gaggagaaaa tgaacgttat ttagaaatct
    17881 ggaacttagt attttcagaa ttcaaccata agcctgataa cacatttgaa ccacttcctc
    17941 ataaaaacat tgatacgggg atgggattgg aacgcatggt ctcaatcgtt caagatgcgt
    18001 caacgaactt tgagacagac ttgtttatgc ctattatcca cgccgtagaa aagctaagca
    18061 aacaagtaaa atatggtgaa aacccagtaa cagatatttc ttacaaagta atcgcagatc
    18121 atattcgcgc cctttcattt gctattggcg acggtgcatt gccatcgaat gaagggagag
    18181 ggtatgtctt acgccgtttg cttcgtcgtg ctgtcatgca tgggaaaaaa ttaggtattg
    18241 aacaagcatt cttatatcgt ttagtccctg ttgttgggga aatcatggta agttattatc
    18301 ctgaagtact tcaacaaaaa gaatttattg aaaaagttat ccgtacagaa gaagaacgtt
    18361 tcaatgaaac aatcaatgaa ggtttggaaa tcttaaatca agtgatccaa gaagtaaaaa
    18421 acgaaaacgg gacgactcta gcaggaaaag acatcttcaa actatacgac acatatggct
    18481 tcccggtaga attaacggaa gaggtggcag aagatgaagg actgaaagtt gatcatgctg
    18541 gttttgaaaa agaaatggat gcacaacgcg aacgtgctcg ctctgcccgc agtactgaaa
    18601 aatcaatggg ggtacaatct gctttattga cagatatcaa agtagaaagt aaattcattg
    18661 gatacacaga acaagaatca gatagtcaat tattggtcat cgttaaaggt gagtctcttt
    18721 tgaatgaagt cgcagaaggt gaagctcaaa tgatctttga tcaaacacct ttctatgcag
    18781 aaatgggtgg gcaagtagcg gatcaaggat ggatcatgaa tgaagtaggc gaaatcgtag
    18841 cagttgttac agatgttcaa aaagcgccta atggtcaatt tatgcacacc gtagaagtaa
    18901 aagcaccaat gagtgaagga caaacctatc atctattgat cgatcaatcg atgcgcaatc
    18961 ggatcatcaa aaaccataca gcaacccatc tactgcacaa ggcattgaaa gaagtacttg
    19021 gcgagcatgc aaatcaagct ggttctttag tcgctcctgg acatttacga ttcgacttta
    19081 cccactttgg tcaaatcact tcagatgaat tagctgaaat ggaaagaatc gtcaacgaaa
    19141 aaatctggga agctatccca gttgaaacaa ttgaaacaga tatcgataca gccaaaaaca
    19201 tgggtgcaat ggctttgttt ggtgaaaaat acggacatga cgttcgagta gtaaatattg
    19261 gcggctggtc aatcgaactg tgcggaggca cacatgtagc aaatacagaa aacatcggaa
    19321 tcttcaaaat cgtttctgaa tcaggaattg gtgcaggcgt gcgacgaatc gaggctgtaa
    19381 caagtaaaga agcgtacgaa ctacttgaag aagaagaaaa acaactaaaa caagtagcaa
    19441 caattgttaa atcacctcaa ttgaaagaag ttgtttcaaa agcggaacaa ttgcaacagc
    19501 aagtaagaga gctacaaaag gaaaatgaac aattagctag caagttagcg aatcaacaag
    19561 ctggtgatgt attcaaagat ccagaaacga tcaatggaaa aacaatcatt gcagcacaag
    19621 taaaagtgaa agatatgaat cagctgcgtc aattagctga ccaatggaaa caaaaaaata
    19681 tttcagatat tcttgtttta gcaaacgaac aagaaggaaa agtcagtctg ctagcggcaa
    19741 tgacgaaaga atcaaatgaa gctggactaa aagctggcga cttgattaaa gcaatcgcac
    19801 cgaaagtagg cggtggcggt ggtggacgtc cagacatggc acaagctggc gggaaaaatc
    19861 ctgctggtat tccagatgca cttaaagctg cgaaagaatg ggttgcgcaa caatagggcg
    19921 tttacgtttg acagaaaaac aaaagttcaa acattgaacg aaacatgatt atgggggcga
    19981 agacaaacaa gtcttgccct catacttttt tcaattggat ggcgcatcta atcaaatggt
    20041 cggattcgga tggtctttag ttgacgaaag tgacagaaat ttttacgatt agtataaata
    20101 aaaaggggtg aaaaaatgaa tagtagaaat aagacctttc gactagtctt acgtgcgatc
    20161 ttgttagcaa tcatcatcgt acaagcgatg gttccttggc tggggtttat tccattagga
    20221 ttcattagtt taacaattat ccatatcaca gtaattattg ctgcagtagt attaggccca
    20281 aaagatggca tggtgattgg cttgttttgg ggaatcgcta caattgttcg tgcctatgcc
    20341 atgccaacaa cgccatttga tacattagtg ttcacgaatc cgattatctc ggtagttccc
    20401 agagtacttg ttggcttagt agcaggcttg gttttccact ggatttatca aaaaaataaa
    20461 tcaataacga tcagctcagc ctttgcaggg gtactgggtt cattggtcaa tacagtctta
    20521 gtgttaggat tcatgggatt attttataca ggtgcaacgg cagatgccta tggtgtagac
    20581 tctgctcttt tatttaaaac cttagcaggg gttgcagcaa tcaatgggat accagaagca
    20641 ataggtgcgg gaatcataac accattacta gcaaaagcgt tatttgcagc aacaccgttg
    20701 aaaccagaat aatgaccatc ttttaatagt aataggacgc acgatagata gaaaaaggaa
    20761 gagaggagag gaaaacaatc gtcctctcca tctttttacc tgagcaacaa gcaaactaga
    20821 aggggtaaaa aacttggcta agaaacaaga agaaaaaaag aaacaaatac cgcaacagcg
    20881 gacaaaaaaa ggagtactag cgggtatctt aggattggtt acgaatctga tcttgtttgt
    20941 ctctaagcta atgatcgggc ttttttcagg aagtgtctcc attatggctg atgcagtgaa
    21001 cagtctatca gatactgctt catccatatt gactttgatc ggatttaaaa ttgctgcaaa
    21061 gccagcagat caggaacatc cttatggaca cgaacgcttc gaatatatca gtgggttatt
    21121 tgtttcgatc atcattactt atgtaggatt tcaattttta gagtcttctg ctgaaaaaat
    21181 catccatcca gaaaatgtct ctttaacacc catcgtcttt tttgtcctaa tcatttccat
    21241 ctttttgaaa tttgcacaag gcaaaatgta tcaggttatt gcgcaatcca ttgattctga
    21301 aacattgaga gctacagcaa cagatagtta taatgatgtc tttactacac tggctgtatt
    21361 gctttcagca gcaatcgaat gggtaactgg atggcgtatt gatggatata taggttttgc
    21421 tttagcatta tatatccttt atagtggatt gacgatgctt cgaaattttg tatacgaact
    21481 attaggaagt cgcccaacgc cagcagagat tgaagaaatg gaagagcatt taaatgcttg
    21541 cgatgatata cttggctatc atgatttatt ggtgcataac tatggaccca ataaacgctt
    21601 tgcatcagtc cacatcgaag tagatgaatc aatgaatttg aatgtggctc ataagattat
    21661 tgatctgata gaaaaagact ttaaaaagtc tttggatgtg gaacttgtct gtcacttgga
    21721 tccaatgcct gttgataacg aaaaatacta taatatcttg cggcagttaa agcaaattct
    21781 tcttgaaaca ggtacagggc ttaatattca tgattttaga gtaattcatc aaggaaaaac
    21841 gctgcaattt gatgtagtgg tccctgagaa attccagttt gttgatacag aactagaaag
    21901 aatcttacgt agtcaaatag aagaaaaaat cggagattac caattggaaa ttacgtttga
    21961 tcacaattat cttttatatt aaatgagaaa ctataaaaag actccttaaa gtttttaatt
    22021 taaagagtct ttttttcttt taattgttta tggagagcct gacgtgcaag attatctgct
    22081 cctttatttt tgttttcggg caaccattgc aggatcaata aagaaaattc ggatagaagt
    22141 tcttggattc tttgaaggta gggaaggaag agctcgttct tcgttgcgtt tttgtcgacc
    22201 gtagaaacta gtgttttgct gtctgaaaaa cagaaaatcg tttcattctg taagtcgttt
    22261 ttctttagaa attctaaagt ttttaaaaag acttcgaact ctgcttgatg gtttgtccct
    22321 atagaaagcg ggatagatag ctgttcttgt ttaccttgat agacaatcaa gattccacct
    22381 ccactaggac cagggttgcc ttttgtagag gcatcaatgt atacttttaa cataaaaaga
    22441 gattctcctt tttgttcaaa ctaatgttat ggtaagatta tatcattact gaatcggtga
    22501 gaggttagag aatgaaaaaa aatattcgat ggcagcctga actggcacat acgatcatct
    22561 attggtcatt gaccttgatc attctgtttt ttagtctgat tctttcttta gaaaacacag
    22621 gtccttattg gaaaagcaat tttgttttag gtatttttct tgtttttcta tttctaggtt
    22681 tgcggcgttc ttttatttta aaatcaaact ctgtgattat acgttatgct gctttttgga
    22741 aagaccgaga aatatctgtc ggacagattc gatctttgac gccaactaat actggggtgc
    22801 aaatgatgtt gcaagatgag aaagccccat ctgttttcat gatgcgaaaa aaagatcaag
    22861 tagtcctttt tgatcatttt gaaaaaaacg gacccaaatc agaatcaatt ccttatcctt
    22921 atacgaaaaa aagaaaaagc taattttaaa aatcagaacc taaaaaaacg aatttttaca
    22981 ggaaaagaaa agagtgagac gtacggttca tcccgtttag cgtctcactc tatttgttta
    23041 tctattcatg catactagta tcggtagtct gaacgttgcc tttcttgaca tgggcagctt
    23101 gtgttcctcg attcccttcg atgatctcaa attcgaccac ttgattttct tccaaagttt
    23161 tgaatccctc ttcttcaatc gcagtaaaat gtacaaaaat ttcttctgtg tcatcataac
    23221 tgataaagcc gtatcctttt ttgttgtcaa accattttac aattcctgtg gtcaaagcac
    23281 tcacgacctt tctaaagtct gatcatagcc ttcattataa agagtattta taagtacgtc
    23341 aaataaatta aaaacctgta attttatgtt ttttttctga ataaaaaaag gtataattgg
    23401 catatcttaa caaagaagat attttgttga ataaagttcg tgaaaagggg taatcaaatg
    23461 aagaatgatc tgcaaatttc acaagaaaca gagctagaac cgattgttga agtggctagt
    23521 aaattagatc ttaaggaaga cgatttggaa ctgtatggga aatataaggc gaaaatcaat
    23581 ttttctggaa tcaatcgatc aaaggataac gctgaggggc atttgatctt agttacttcc
    23641 atcaatccaa caccagctgg cgaaggaaag tcaacgatca ctgtagggtt aggagacgcg
    23701 ctcaataaaa tcaataaaaa atctgttatt gcattgcgag aaccctcact tggtcctgtg
    23761 atgggaatca aaggtggtgc tgcaggcgga ggatatgcac aggtattgcc tatggaagat
    23821 atcaacttgc attttactgg tgacatgcat gctatcacaa cagctaataa tgcattatct
    23881 gctcttttag ataatcatat tcatcaagga aatgaattga acatcgatgc ccgccgagtg
    23941 atttggaaac gggtagtcga tctcaatgat cgtgaactgc gtaaagtaat tgttggcttg
    24001 ggtgggccaa tccaaggtgt tccaagagaa gatgggtttg atatcacagt tgccagcgaa
    24061 atcatggcta tcttgtgtct tgcggcagac cttgaagatt taaaggctcg tcttgcaagg
    24121 atcgttgtcg gctatacatt tgaccgtcag cctgtgactg ctggagactt gaaagcagaa
    24181 ggcgctctgg cattactgct gaaagatgca atcaagccga atcttgtaca aacaatttac
    24241 ggaacaccag cttttgtcca cgggggaccg tttgccaata tcgctcatgg ctgcaatagt
    24301 attttagcta ccaagacagc attgagatta gcagattacg ttgtcacaga ggcaggattt
    24361 ggtgcagatc ttggcggaga gaaattttta gatatcaaag tacctaactt gaaaaaagcg
    24421 ccggatgctg tggtgattgt cgccactgtc cgtgcattga aaatgcatgg cggaatgaaa
    24481 aaagatgaac ttaaaaatga gaatcttgat gctttgaaag taggttttgc aaatttaaag
    24541 cgccatatac gtaatatgga acaatatcag ctacctgtga tcgtcgcaat caatgaattc
    24601 gtaacagata cagacaatga actcacactg ttggagcact tgtgtgaaaa tcaggggatt
    24661 cttgccaaga gagcgagtgt atgggcagat ggagcagaag gcggcgttga tttagcaaaa
    24721 gctgtagtac gattgattga tagaaaagaa gctaattaca agccattata ccgcctagaa
    24781 gaaacgatac aagaaaagac agaaacaatc gtcaagaaaa tttacggagg aaatggtgtt
    24841 gttttcagca aaaaagcgca aaaacaaata gaagagttta cgaagaatgg atgggacaaa
    24901 ctgccgattt gtatggcaaa aacacagtat tcattttctg atgatccttc actgctaggt
    24961 gcaccggaag atttcaccat cacgatccgc gaaatcatcc caaaattagg ggcaggattt
    25021 ttagtagcct tgactggaga tgttatgacg atgcctggtt tgcctaaaaa acctgctgca
    25081 ttgaatatgg acgtgacaaa tgacggagaa gtgttgggat tgttttaagc ttgattataa
    25141 tctaccatcg aaggagctgt attttgacta cagcttcttt ttttagacga tagggaatta
    25201 attttgttta cactaggatc attctttttc tttattgtat tttgagctat actaaagcat
    25261 aataggaggg tatgatgaaa acgacattta taagaaaaac cggtttttac ggtatgggta
    25321 gctcgattca gctaagaatc aacaatgaaa aacgcagtgt tctcagtcat gaacaaaaga
    25381 taacgcttga tctggaagtg ccctttacga tgcaggtcac cttttattgg ctaaaaagcc
    25441 ccgtttatac agtgaaagaa gcaaagcctg cttatgtgat cacaatgaat tacttgattc
    25501 ttcaaatcta tccgttttta tttctagcaa ctgggttggg ggccgttttc ctacaaaatt
    25561 tatttttcag tttttttgca gttattgtgt tgattgcgtt ctttttctat ataaaaaacc
    25621 gggcatattt gatcaaggag gaaaattatg gcaaactctg atcttttttt aatcagtttc
    25681 aatcgaatcg aaaaatggat gagagacgag atgggaaatg cacgaaatat gggatttacc
    25741 gaacttgtca gacgcctagc tcaaagaaaa caattaatga tcagaaaata cgaagatgat
    25801 cttttacagc ttgctcagtt aaggaatgcg atcgtccatg atcggatcgc agtcgatttt
    25861 gtcattgcag agccaaatga ttggacagtc aagcgaatcc agctgatcga acaagaactg
    25921 attcagcctg aaaccgtgct gcctcgattt gcaaaacgtg taacaggttt tgaacaagat
    25981 cttccgttac ttgaactgtt aaagatagta gcagaaaaaa ggtattcaca atttccgtta
    26041 tacaataaag gaaaatttgt agcattgatt actttacgta atatcgggtt ttggctagca
    26101 aaagaaagcc aaaaaggtcc ggttgattta acaaataaac gagcgttgga tttgatcatc
    26161 caaaacggaa agtacacaaa ttatcatttt gttccagcta aaacacatat ttacgaggtt
    26221 gaagcgatgt ttcgtgagca agggactttg gaagccgtac ttatcactaa agatgggaat
    26281 cccgatggta atctgctcgg gatcgtacga cctcgtgata tttataaaca aatagaaaag
    26341 gattgatttt ttgttaatta tttattggat attcagcgct ttgctagtag gagtggatca
    26401 gttggtcaaa tggtggatca ctgataatct agcgcttgga gagacgaaaa atcttatacc
    26461 aggtattttg tcactgaatc atattcgaaa cactggagcc gcatggagta tgctagaagg
    26521 gaagatgtgg ttctttacta tagtgacact cattgcagtc gttgtgattc tcacattgat
    26581 gattaaaaat cgtgaaaaag gtaatcgctg gttcatgatt ggattgagtt tgattctagc
    26641 tggtgcgatt ggcaatttta tcgaccgtat tcgtcttggc tatgtagtag atatgttcca
    26701 gacagatttt atgagctttc cgatttttaa cgtagcagat gtgacacttg tatgtggagt
    26761 gatttgtatg ttaatttata ttattttaga tgaaaaggat caaagaaaaa aatgacagaa
    26821 cttaaaacga cgattcacca tgaatctggt aggatcgata aagtattgac taatttattc
    26881 accgattaca gtcgttcgca aattcagctg tggctgaaaa atggagcggt ttctgtcaat
    26941 ggacagccag tcaaagcaaa ttacaaagta aaacaaggag acgagattac tgtcttgatc
    27001 cccgaaccag aaacattaga tgtggtccca gaaaatatcc cgttgacgat cgtttacgaa
    27061 gatgaagatg tagcggtgat aaacaaacct caaggaatgg tcgttcatcc ttcagcaggt
    27121 catacgcatg gcacaatggt caatgcgctg atgtaccata tcaaggatct ttctacgatc
    27181 aacggcgtta ttcgtccagg tattgttcac cgaatcgaca aagatacttc cggactgcta
    27241 atggttgcca aaaatgacca ggcacacgaa tcattagctc agcaattaaa agataagacc
    27301 tctttgcgca aatatgttgc tttagtccat ggagagattc ctcacgaaaa aggaaccatc
    27361 aatgcgccaa tcggccgttc gaaggaaaac cggaaaatgc aggcgataag agaagatggg
    27421 aagtcggctg tcacacattt tactgtattg gaacgtttcg aaggatttac tctggttgaa
    27481 ttaaaactag aaaccggaag aacacaccag atccgtgtgc atatgaagta tatcggttat
    27541 ccgttagctg gagatccagt atacggaccg aagaaaactt tgaaaggcaa tggtcagttt
    27601 ctccatgcgc aaatgttagg ttttacgcac ccgactacaa acgaaaagat gatctttgaa
    27661 gcacctcttc cagaagtttt tgaaaaaact ttagaaaaat taagaaaaaa cattgcattt
    27721 taatttagat aacggtatag taattaacaa cgaaagcata aaacttaata gaattccttt
    27781 aagtttagtc ccgtgaggct aagaagggtg ggagcaagga tacgtatggt gcgccataag
    27841 tagattgctc attgacatag tatagctatc agtgtatcag tatacgctca gtctaaacat
    27901 tcctcctgtc ctcttagggc aggagttttt ttatgcaaaa aagtaggggg aatgaaaatg
    27961 caagcaaaag aagtagtaga tcaggtaacc atgaagcgag cattgactcg aattacttat
    28021 gagattatcg agagaaacca tagtatccaa gatatcgtat tggtaggtat caaaacacga
    28081 ggaatctaca ttgcatcaag aattgccgaa cgcctgaagc agctagaaga tatcgatatt
    28141 cctgtaggag aactggatat cactttatac cgagatgaca aaaaagaaaa tccagaagaa
    28201 ccggaacttc attcttctga catcccagtt tccttagaag gaaaagaagt tattttgatt
    28261 gatgatgttt tgtatacagg acgaacgatt cgagcagcaa tggacgcagt aatggatttt
    28321 ggcagaccaa gaaaaatttc attagcagtc ctggtcgatc gtgggcatcg agaattgcca
    28381 atccgtgcag attatgttgg gaagaacatt ccaacatctc gggcggaaga gattttagta
    28441 gagatgcaag agcttgatgg acaagatcgc atcatgattt tgaaagagga agattagagg
    28501 agagagaaac atgtcggaga aaaaaggatt tcataatgat gacgtggtgt tagatatcca
    28561 cgatcgccca accccaggac attgggtagg attaagttta caacacttgt tcacgatgtt
    28621 cggagcaacc gtgttagtcc ccatcttagt aggtatcgat ccaggtattg cgctagtcag
    28681 ttcaggacta ggtacattgg tgtacttatt tactactaaa ggaaagatac ctgcgtactt
    28741 aggaagcagc ttcgctttca ttgcagcgat gcagcttttg atgaaaacag atggttatcc
    28801 agcgattgca caaggagcag tcacaactgg attagtctat ttgattgttg cgctgatcat
    28861 caaaaagatc ggttcagcat ggctggataa aatcttgcct ccaatcgtcg tcggtccagt
    28921 catcatggtt atcgggttgg gacttgcggc aaatgcagca acaaatgcta tgtataatca
    28981 gggcacgtat gatttcaagt atattgctgt agcgctgatc acattaggat taacaatttt
    29041 cttcaacatg tggctaagag gttttctcgg attgattccg attttactag gtattattag
    29101 cggatacatc gtagccttgc tgtttggaat cgtggatact aagccaatcg tagacgctgc
    29161 ctggtttgct gttcctaact ttgagattcc ctttgtacaa tatgaaccaa agctatatct
    29221 tggagcaatc acaacaatgg cacccatcgc ttttgtgacg atgacagagc atatcggcca
    29281 tcttatggta ttgaataaac taactaaacg taatttcttt gaagaaccgg gtcttcataa
    29341 gacgttgaca ggtgatggat tagcacaaat cgtggcagcc tttgtcggag gtcctcctgt
    29401 aacaagttac ggtgaaaaca ttggtgttct ggctatcaca cgtgtccata gcgtgtttgt
    29461 gatcggtgga gctgcagtat ttgcagtcat tttaggattc gtaggaaaat taagcgcttt
    29521 gattttaagt atccctggac cagtcatctc aggaatcagt tttatcttat ttggagtaat
    29581 tgccgcaagt ggaatgaaga ttttaatcga gaacaaaatc gattttgata aaaagaaaaa
    29641 tctgttgatt gcttcagtta tcttagtagt aggaattggc ggactcgtct ttgaagtagg
    29701 cacatttaca ttgtcggcaa tggcattagc aactgtactt ggcatcattt taaatcttat
    29761 cttaccagaa acttcacgaa gcgaagaaaa ctaaggagga aacattatga tcatcaaatc
    29821 agaacgtatc agcttaaagc atttattgac cgttgaggca cttactgatc aagaagttat
    29881 gggcttgatc cgcagagggc aggaattcaa gcaaggtgct aattggactc cgcaaaagcg
    29941 ccagtacttt gctacaaacc tctttttcga aaatagtact cgcacacata aaagcttcga
    30001 tgtagcggag aaaaaactag ggctagaagt gattgaattt gaagcaagca caagctctgt
    30061 ccaaaaaggg gaaacactat acgatacagt actgacgatg tcagcattag gcgtagacgt
    30121 agcggttatc cgtcatggtg atgagaacta ttatgacgaa ctgatacaaa gtaaaacaat
    30181 ccagtgttcg attatcaatg gaggagacgg aagcggacag catccaaccc aatgcttgct
    30241 tgatttgatg accatttatg aagaatttgg tcattttgaa ggtctgaaag ttgccatcgt
    30301 aggtgatatc actcactctc gcgtagctaa atccaatatg cagatgttaa aacgtctagg
    30361 cgcacaagta ttcttctcag gtcctagaga atggtacgac gatgaattcg aagtgtacgg
    30421 acattatatg ccattggatg aactactaga tcaagttgat gtcatgatga tgcttcgggt
    30481 gcaacacgaa cgccatgatg gaaaagaaag tttctcaaaa gaaggctacc atcaggaaca
    30541 tgggttaaca gttgagcgtg cgaagaagtt gcaaaaacat gcaatcatta tgcacccagc
    30601 accagttaac cgtgatgtag aattagctga ttcattagtg gaaggattac agtcgcggat
    30661 cgttcagcaa atgagcaatg gagtattcgt cagaatggca atcttagaag cagtgctaaa
    30721 tggaaaagcg taattatagg aggatgaaaa aatgaaaaca ctcattaaaa acggtcagat
    30781 caatacacga aaaaatatga cgacaccagc agaaatttgg atagaagatg ggcgaatcaa
    30841 agcaatcggt acaggattct cagaagccga atttgatgaa gtgtttgatg caaagggaca
    30901 attgatcaca cctggtttag tggacgtcca tgttcatttg agagagccag gatttacata
    30961 caaggagacg atcgaagctg ggactcgctc agctgctcgt ggcggtttca ctactgtatg
    31021 cgcgatgccg aatttaaatc ctgtacctga tacggcagaa aagctgagac aagtatatga
    31081 catcattaga aaagatgcag ttgtgaaggt attgcagtat gcgccaatca cagaaaatct
    31141 gcgaagtgag aaattagttg atcaagaagc actgattgaa gaaggtgctt ttgctttcac
    31201 aaatgacggt gtaggtgtcc aaacagctgg gacgatgtat ctggctatga aagaagctgc
    31261 aaaaaacaat aaagccctag tagcccatac agaagatgaa tctttattat ttggcggcgt
    31321 tatgcacgct ggcaagaagg cagaagaact tggtcttccc gggatcttaa gcgtaaccga
    31381 gtcttctcaa attgctagag atctgttgct agctgaagca actggtgttc attaccatgt
    31441 ttgccatgtt tctactaaag aaagtgtccg tgtaatcaga gacgcaaaaa aagcaggaat
    31501 ccacgtaaca gcagaagttt caccccatca tcttattttg atcgacgaag atattcccga
    31561 agatttcggc ttttggaaaa tgaacccgcc gctaagagga cgggaggaca gagaagcttt
    31621 gatcgaaggc ttgcttgacg gtacgatcga ttgcatcgct acagaccatg ccccccatgg
    31681 attagaagaa aaaagccaaa gtttcatgaa atcaccattt ggtatcgtag gaagcgaaac
    31741 agcgtttcaa ctgatctata ctcattttgt agaaactggt cgattcacgt tggaacaagt
    31801 gatcaattgg ttggcagtta aaccggctga gatttttggt ttgaatgcag gaacgctaac
    31861 ggttggtgcc cccgctgatg tagcagtttt tgatattaca caaacttgca cgatcgataa
    31921 agaagacttt ttatcaaaag gggagaatac accttttatc ggctggaaag taaaaggcga
    31981 aacacaaatg acttttgtca acggaaaact tgtgtggcag aagggagaat aaaagtatgg
    32041 aacgtttgct tatattagaa gatggaacag ttttcgaagg aaaggctttt ggtgcagatg
    32101 ctagtgtctt cggagaaatc gtttttacaa caagtatgac tggttatcaa gaaactatca
    32161 ctgatcaaag ttttaacggt caaatcatta catttaccta tcctatggtg ggtaattatg
    32221 gggtaaatag agatgattat gaatcgattt ctccaacatg caaaggagta gtagtaaaag
    32281 aacatgcacg tgtagcttca aactggcgaa accaaatgac gctagattca tttttgaaac
    32341 aaaaaggaat tcctggaata tcaggtatcg atacgcgcgc ccttactcgc aaaatccgtg
    32401 aagtcggaac gatgaaagct agtatagttg atgctggaga ttccttcgaa catgcatttg
    32461 atcagctgaa agcaaccgtt ctagcaacca atcaagtgca gcaagtctca actaacaaac
    32521 cttatccaag tccaggaaca ggtcggaaag tcattgtcat tgacttcggc ttgaaacata
    32581 gtattttacg agaattgagt aaaagaaact gtaacctcac agtacttccc tataacacag
    32641 atgccgagac gattttagcg ctatcaccag acggagtcat gctgacgaat ggacctggtg
    32701 atccaaaaga tgtgccagaa gcaatcgaaa tgatcaaaaa aatccaagga aaagtaccga
    32761 tttttggtat ttgtctcggt caccagttat ttgctttagc caacggtgca gatacctata
    32821 aaatgaaatt cggacatcga ggattaaatc acccggtacg agaagtagcg accggaagaa
    32881 ttgattttac ttctcaaaat catggctatg cagtcgatga agccacgatt gatccagaaa
    32941 aactcatcgt aactcatgta gaagtaaatg acggaacagt agaaggagtc aaacacagag
    33001 attatcccgc tttcagtgtg cagtttcatc cagatgctgc ccctggtccg catgatgcac
    33061 tacatttatt tgatgaattt atggaaatga tggacgcagg aaaggagcag taaacagatg
    33121 ccgaaacgta cagatatcaa gaaaattatg gtgattggat ctggtccgat catcattggt
    33181 caagccgcag aatttgacta cgcaggaaca caagcctgcc ttgccttaaa agaagaagga
    33241 tatgaagtgg ttttagtaaa ttctaatcct gcgacgatca tgacggataa agaagtggca
    33301 gatcaagtat atatcgaacc gatcacactt gaatttgtat caaggattct tcgaaaagag
    33361 cgtccagatg ctctccttcc aactttaggc ggacagacag gcctcaatat ggcaatggag
    33421 ctggcagcat caggtatttt agaagaattg aacatagaac ttctaggaac aaagctttct
    33481 gcgatcgatc aagcagaaga tcgtgatcta ttcaaacagt tgatgaaaga attgaatcag
    33541 ccaatccccg agtctgaaat cgtcaataca gtagaagaag ctgtagattt tgcaaataaa
    33601 atcggctatc ctgtcatcgt tcgtccagcc tttactttag gcggaactgg tggaggtatg
    33661 tgtgaaaatg aagaagaatt acgtatcgta tcagaaaatg gattaagtct ttcccccgct
    33721 acccaatgtt tgattgaacg aagtattgca ggttttaaag aaattgaata tgaagtgatg
    33781 agagattcgg ctgataatgc aatcgttgtc tgcaatatgg aaaattttga cccggtgggt
    33841 atccatacag gtgattctat cgtatttgcc cctagtcaaa cattgtctga ccatgaatat
    33901 caaatgcttc gtgatgcttc tctttcgatt attcgagcac ttaagatcga gggaggctgt
    33961 aatgtccaac tcgctcttga tccacatagt tttaattatt atgtgattga agtcaatccg
    34021 cgtgtttctc gttcatctgc gttagcaagt aaggcgactg gctatccgat cgctaagcta
    34081 gctgctaaaa tagcgcttgg attaacatta gacgaaatga aaaatcctgt tacaggaacg
    34141 acttttgcag aatttgaacc tgcattagat tatgttgtag ctaaaattcc tcgctggccg
    34201 tttgataagt ttgaaaacgg agaacggctt ttaggcacgc agatgaaagc aactggtgaa
    34261 gtgatggcta tcggtcggaa tatcgaagag tctttattga aagctgtgcg ttctttagaa
    34321 atcggtacac accatatcga acttcctgag ttggcagaca tcacggacga tgacctaatc
    34381 caaaaagtgg ttcgagcaca agatgatcgc ctgttttatg tagctgaagc tattagaaga
    34441 gggtacacca tagaagaatt agcggatttg acaaaaatcg atttattctt tttagataaa
    34501 ctgttgcata tcgtagaaat cgaacagcag ttgaaacagc agccgttgga tgacaagata
    34561 ttgaaaacag cgaaacaaaa cggttttaca gacaaaaaag tggcggagct gtgggaaata
    34621 aacgaagaag ccgttcgcca gatgagaaaa gaaaatgatt tgattcctgt atataagatg
    34681 gttgacactt gtgcagcgga attcgaatca aaaacacctt atttttatag ttcctatgaa
    34741 tatgaaaatg aaagttcacg atcagaaaag ccctcaattc tagtactggg atctggtcct
    34801 atccgtatcg gacaaggggt tgaatttgac tatgcgactg tgcattctgt caaagcaatc
    34861 cagcaggcag gatacgaagc aatcatcatg aacagcaatc ctgaaacagt ttcgactgac
    34921 ttttctattt cggataaatt atattttgag ccattgacct tcgaagatgt gatgaatgtg
    34981 atcgagctag aacagccgat aggagtaatc gttcaatttg gtggtcagac agcaatcaac
    35041 ttagccgaac ctttgactgc tgctggtgta aaaatcttag ggacaacgat cgaagacttg
    35101 gatcgtgcgg aaaatcgtga tttattcgag caagcgctga aagagttgga tattcctcaa
    35161 cctttagggg atactgctac aagtaaagaa gaagccgtgg ttatcgcttc gagaattggt
    35221 tatccagttc ttgttcgtcc aagctatgta ttaggcggac gtgcgatgga aatcgttgaa
    35281 aaccagcgag atttagaaga ttacatggaa catgctgtga aagcatctcc agaacatcca
    35341 gtactagtgg accgttactt gcttggtggt gaatgcgaag tggatgcgat ttgtgacggt
    35401 gaaacagtac tcatcccagg gatcatggag catattgagc gtgctggtgt ccattcaggg
    35461 gattcaatgg ctgtgtatcc acctcaaaca ttatctgaat cgatcaagaa aacgatcgaa
    35521 gattacacga tccgcttagc attaggtttg aattgtgtcg ggatgatgaa tatccagttt
    35581 gtcatccatg aaaatgaggt ttatgtgatc gaagtcaatc ctcgagcaag tcggactgtt
    35641 ccattcttaa gtaaggtgac aggaattcca atggcacaaa ttgcgacgaa agctattttg
    35701 ggagagaaac taaagacact tggttatgaa aacggacttt atccagaaac aaaagaagtt
    35761 catgttaaag cacctgtctt ttcttttacg aaattgcaga aagttgatac ttacttaggg
    35821 cctgaaatga aatcgactgg agaagtaatg ggatctgaca gacaattaga caaagctttg
    35881 tacaaggcat tcgaagcttc tggattacac ttgcctgaat atggagccgt attatttacg
    35941 attgcggacg aaacaaaaga agaagcacta gaattagcta aacgttttac agaaattggc
    36001 tatagtctga taggaacgaa acatacagct gcttatttcg aaaaaaatgg tctggtcgtt
    36061 gaaacagtag cgaaaatttc tgaggaagca gcagaaaaaa atgtagtcga cttaataaga
    36121 gatggtaaaa cacaagtggt tgtcaatacg attgataaag atcggggaaa tgcttctaaa
    36181 gacggtttca tcatccgaag agaagcagtt gaacatggta ttccattgtt tacttcttta
    36241 gacacagcag atgcgatcgt tcgagtgatg gaatcccgtg ctttttcaac taaccctatt
    36301 taacggcgag cacatgttaa gacaaaaaga cgaaaaaccg ttgcaggagg ggacagatga
    36361 aacaagaaat gatggaaatc gttcagcaaa aacagctggc accaaagatt tttgaaatga
    36421 cattgaaagg tgatctagta aaagaaatgc agcaaccagg acagttcttg catattctcg
    36481 tgccgcgtgc agacttgcta ttacgccgac cgattagctt gaatcatatc gaccacgaaa
    36541 caaatacttg ccgtatcatt tatcgggtgg aaggtgaggg gactagagtc ttctctactc
    36601 tttctgccgg agaccattta gatgtcatgg gtcctttagg caatggtttt gatttgtcca
    36661 atcttcatac aggagacgaa gtatttataa tcggtggagg aatcggtgtc cctcctctat
    36721 atgagttatc aaaacagtta gtcgaaaaag gaattaagcc aacgcatttt ttaggttttg
    36781 ccacacatga agtcgtttat tatgaaaaag aatttaatcg tctaggagaa acaagaatcg
    36841 ctactgacga tggaacatac ggtatgcacg gaaatgtcgg taatttattg ttaggaacga
    36901 agacggaacc tacagctgtt tttgcatgtg gttcaaacgg gttattgaag acagtggaac
    36961 agctgttttc ttcgcatcaa aatgtccagt tatcattaga atcccgcatg gcatgtggga
    37021 taggggcttg ctatgcttgc gtttgtcata cgccggaaga tgaaacagga acaaaaagtg
    37081 tgaaagtttg tgacgaagga ccgattttta aaataggaga ggtaattata tgaatgagaa
    37141 tcctttagcg gtcaaacttc ctggcctaga attaaaaaat cctatcatgc cggcaagcgg
    37201 ctgtttcggt tttggcaaag aatacggaaa atactatgat ttgaatcagc ttggatcgat
    37261 catggtcaag gcaacgactc caaatgcccg ctttggcaat cccacgccaa gagtggcgga
    37321 aacaccatct ggcatgttga atgcaattgg tttgcaaaat cctggtctag acgttgttat
    37381 gcataaactt cttccagaat tagaggagta caatgagtta cccatcatcg caaatgttgc
    37441 tggtgcttgt gaagaagact atgtagaagt ttgtagcaaa attggcgaag caccaaatgt
    37501 gaaagcaatc gagctgaaca tttcttgccc aaatgtcaaa catggcggaa ttgcttttgg
    37561 aacagattct gatgttgctt ttcaattaac acaagcagta aagaaagttt cttctgttcc
    37621 tgtctatgtc aaattgtccc caaatgtaac agacatcgtt ccaatcgctc aggcaatcga
    37681 agctggaggg gcagatgggt ttacgatgat caatactctt cttggtatgc ggattgacct
    37741 taagacaaga aaaccgattt tagccaatca aacaggtgga ctctccggac cagcaatcaa
    37801 gcctgtcgct attcgtttga ttcaccaagt agcaagtata tccagccttc caatcattgg
    37861 gatgggcggc gtgcagacag tggatgatgt tcttgagatg tttatggctg gagcaagtgc
    37921 tgtagctata gggaccgcga atttcaccga tccgtatatc tgtcctaaac taattaaaga
    37981 gctccctgtg cgaatgagcg aactaggcat cgaatcgctg gaaagattaa tcaaagaagt
    38041 gagagaggaa cgtgaacgat gaatcaacta gaccaacgac caattattgc attagatttt
    38101 tcaactaggc aagaggtaga agactttctt cgatttttcc cgaaagaaga aaaattgttt
    38161 gtgaaaatcg gaatggaatt gttctaccaa gaaggacctg aaatcgtacg ttatctaaaa
    38221 gaggctggtc ataatgtatt tttagatctg aaacttcacg atattccgaa tacggtcgaa
    38281 aaagcaatgc ggggattggc aaagcttggt gttgatctca cctgtgttca tgccgctggc
    38341 ggtattcgta tgatggaggc ggcaatgcga ggattagaag aggggactcc agaaggtgga
    38401 aaacgtccgc tacttctagc tatcactcag cttacttcta caagtgaaga agaaatgcat
    38461 gccgatcaac taatcgaagt ttctttagaa aaaagtgtga tccattatgc tagctgtgcc
    38521 aaaaaagcag gattagatgg tgtggtttct tctgcttggg aagtagaagc gatcaaagaa
    38581 atggcaggag acgaatttgt ttgcttaact ccgggtataa gaccagaagg aacagtcgca
    38641 ggagatcaga caagagtagt cacaccttct caggcaaaaa agatcggctc aacttttatc
    38701 gttataggtc gaccaatcac tcagtcaact aatccttata aagcatatca aacgattcaa
    38761 acagaatgga gctaacctaa aatgaatgtt gaacaaagta tcgcaaaaga cttattagaa
    38821 attgaagcag tatttttaaa tccaagtaat ccttttacat gggcaagtgg aatcaaaagc
    38881 ccaatttact gtgataaccg aatcacgatg agctatccaa aagtccgaaa agagatcgca
    38941 aaaggattag caagcaaaat caaagaagct taccctgaag tacaagtgat tgctggaact
    39001 gcaacagcag gaatccctca tgccgcatgg gtagcggaaa tactggattt gccaatggtc
    39061 tatattcgaa gtaaggcgaa agatcatggg aaaggtaacc aaatcgaagg acgtatattt
    39121 gaaggtcaga agatggttgt gatcgaggat ttgatttcta caggcggaag tgtcttggaa
    39181 gcagctgaag cagcaaaaag agaaggtgcg gatatccttg gtgttgcggc tatctttacg
    39241 tatgagttgc caaaaggaaa agcaaatttc gaaaaagccg aaatccctct tatgacgttg
    39301 accaattatt ctgtattgat tgaagcagct ttagaagatc gttatatcga tgagcaagaa
    39361 ttaactctgc taaaagaatg gaaaaaagat cctgaaaatt ggcagattta agcgcgcttg
    39421 atgaagatag agaaatccga tttgtgtctc cagaatcatc aattggaata gcaaaaatcc
    39481 aaaaactgat tgcgagaagc atcttttcat caggtatgaa aagaaacttc tatcgtgatc
    39541 agtttttgat ttttttaaaa ttcatttttc taagactggt tagcagaaag aagagaataa
    39601 tctcttctct tttttctatt atatatagat tttctctatt ttttggtaag ggagctgttt
    39661 caaaaatggt aaagagcgga aatgttgcga atatcagtta tttaatgata tgatattcgg
    39721 aaaaagaaat ctcaaaggga ggtaactttt atggggttga atgaatttat tttaggattg
    39781 aacaatttag agacgatcac aagggcacct ggatttttta aatatacaga gcatacagtt
    39841 gcagcacatt cgtatcgtgt agcatctatt gcgcaagttc taggagacat tgaagaagcg
    39901 aatggacaac cgatcgattg gaaactgctc tatgaaaaat cattgaatca tgactacaca
    39961 gagcgtttta ttggtgatat caaaacgccg gtgaaatacg caagcaaaga gttaagaggg
    40021 atgctgcaaa cagttgaaga aaagatgacg gacgaattca tcaaccaaga gattccagct
    40081 gagttccaag aagtttatcg ccgtcggttg ttcgaaggaa aagatgaaac cgtagaagga
    40141 gaactattag ccatttcaga taaggttgac cttctttatg aatcttttga tgaaatcatc
    40201 aaaaataacc cggaaaaagt ttataaggag atgttcattg aggcagtgac tacgattaaa
    40261 agctttgaac atcggccttc tgttcagtat ttctttaaag agatatttcc ggaactattg
    40321 aaccaggaat tttatggaaa agaggcgttt ttagaaaccg tctcaacgat tgtaaagaaa
    40381 gaagaaaaat aaaaatgata tgagatgcag ggggaagtat agttgaaaaa gaatatggat
    40441 gtcgaatggg gctattctgg agacaaaggt ccggaaaatt gggcgagttt atgtgattgg
    40501 tttgcgagag gtgcggaatt tccattgcag tcgcctattc atttgaccaa gggggaagaa
    40561 accaaaatag aaggagacac gctttccttt cattatcaaa agcaacggtt tactgacaag
    40621 gaattcaaaa acacgattca tttagtacct tttgaccaac tcagttatgt agagttcaag
    40681 aaagaacgct attatttgac agatattcat ttccacttgc cttctgaaca tatcatcaac
    40741 ggaaaacagg aagatattga atttcatttt gttcatatga acagcaaaaa agagaatcta
    40801 gttgtgggtg tcatgtacca gctaatggta caggaaggtt ggctgtataa tcaagaaaat
    40861 ggggaaagtt ggaacttaga aaaacatgag cattgggtaa atccggatgt gttctttccg
    40921 gagcaaaaaa gccatttcca ttatattggc tcattgacaa ctccgcctac cagcgggcca
    40981 atccaatggt ttgtaatgga tcatgtagga aaactgaacc aagagtttct tctccccttc
    41041 gatggtcagt atgctcgacc gaacaaccga ccaatccagc ccttgaatgg aagagaaatt
    41101 tattatttcg aggagtttga gcaatgaata ttcagcagat gaaatacgtg gtcgctgtag
    41161 caaacaatgg tagtttcaga gaagcggcaa agaaaatgtt tgtctcccag ccaagtcttt
    41221 cagctggtat caaagaatta gaaaccgagt tagggatcac gctgtttact cgaacgaatc
    41281 gaggggcata tttaacagaa gaagggcaag acttcttaaa tcgtgccgag aaaatattgg
    41341 tccagcttga tacattagaa actcactatc taacgagtga aaagagagac aatttttcga
    41401 tcgctgccca gcattatgat ttcttaggtc ctttgaccgc acagctactc gaggaattca
    41461 aagacgagat caaacaattt cggatcatgg agacgacaac agcgagagtg atcgaagaag
    41521 tcaaagagca gcacagtgag atcggcatta tttatatgaa tgagcataat caagctggta
    41581 tcagaagata tttacaacaa ggagagctaa gtggccgcag cattggaaaa ttcaaaacgc
    41641 atattttttt aaggcaagga catccattag ctgaacacgc agagatcacc aaagaagaat
    41701 tgttatctta tcctcaagtt cgttttacac aggatggaaa taatttcccg tatttttatg
    41761 aagaccttat cgagatccct gatcaagaaa cagtgattta tacgagtgat cgaggtactt
    41821 tgatgaatat cgttcttgaa actgatgctt acgcttcagg atctggtatt gtgattggag
    41881 ggattcgcga gcacttgcgg ttaatccctt tggcagaagg cgaactaaat gaattttata
    41941 tcatttattc ggcgaaacgt ccgctcagtg aaatcgctca aagatttatc aaagagctta
    42001 ctcggttact ggaccagcag agctaagaaa agcagatgag aacgctgttt attgaacacc
    42061 gttgatcaaa aaagaggggt tgtgacagaa ttttgtcaca actcctcttt ttgtagcaaa
    42121 caatttataa ctaattgctg ttgtgggaaa gtacgcaaat atgattctag aaacactcat
    42181 tgattacctg ttttgtttca tagctgtaat tttttcttct tctggtgtca cgataatcgt
    42241 cttttggttt tcataaataa cataaccagg cttggcgcca tttggttttc ggatatgctt
    42301 tacctgtaca agatcaaccg gcacttgggc agagtatcgg tattttgaaa aataggcagc
    42361 taactcggca gcttctgtaa ttgtttcatc tgagggcttg tcacttttaa tgatgacgtg
    42421 cgaaccgggt atattttttg catgaagcca atagtcggtt tttttagctg tcttcattgt
    42481 cagctggtca ttttgaagat tgtttttacc aacaagaatg agcgttccgt cagaagaaaa
    42541 gtattgatca ggctgagatg gtttcttacg tttttgtttt tttaagttct tctttttcag
    42601 gtatccttct gaagtcaatt cttctttaat tgcttcgata tccattgggc cggcaatttc
    42661 cagttgagat aagacagatt caagatattg gatttcatct ttggcttctt ggatttgttc
    42721 gccaatcagc ttaacagcgt tcttcagctt ttggtatcga tggaaatatt tctgtgcatt
    42781 ttgatttggt gtcaatgccg gatctagtgc tattttgatt ggccgatctt cttcataata
    42841 attaggtaaa acaacttctt ttgcgccgcg tggtacttgt gtcagaaaag tagtaagtag
    42901 ttcgccattt ctacggtaat tctcagcgtt ttcggattct ttgagtgttt gttcacgttt
    42961 tttcaattta tttttatttc gttttagttc attttcgatt tttcgaatca gttctccgcc
    43021 ttgttgtttt gcccgatctt tctctgcttt ttcgtgataa tagctatcta ataattgact
    43081 gagtgttgga taagctgtta cagcagatgc ctgttcggac aatacctgat aagcaatcgg
    43141 tgtgaaaaaa tctttatttt ccgtttcgta aaaagtagga ataggctgat ggctgatggc
    43201 tgagaaaaat tcgtcccata caaccatctt ttcattagga cgttccagta atcttttggt
    43261 cagctcttga gcgatatcga aaccgattcc ctgaaactga cgttggattg cttttggatc
    43321 tagttcagtt tgggaaagcc gctgaaagat tttttctttc tcggagctaa atgggttcaa
    43381 gacctcttgt tttggcggag caacatattc tacaccagga agcaaagaac gataagtgtt
    43441 ttgagagctt ccaatgtgtt tgatcgcatc cagtattttt ccagtttctc gattaactaa
    43501 gacgatcgta ctatgacgtc ccattaattc aacgatcaat atgatatttt gtagatcacc
    43561 caactcgttt cgtttagcaa agtggaaatg gatcactcga tcattttcga tttgttcgat
    43621 gtcttctaaa atagcgccat ccaaatattt tcgaagcatc atcacgaagt taggcggatt
    43681 atcaggattc tgataatcga tttgggtaat ctgtacacga gcataacttg ggtgcgcaga
    43741 taataataat cgatggtttt tgccgcgtga acgaatcacc agcacgattt cattttcata
    43801 aggctgatgg attttcgaga tccggccaga tagtaacgtt tcacgcaatt cgttgaccat
    43861 cgcgtgggta aatacaccat caaaggacat ggttcattcc tcatttcttt tcataagtat
    43921 attcattata acgaaaaaaa gaagaaaaac caatttggcc gaatagtaaa gcaaaagtct
    43981 agaaaaatct tggactaatt acataaaaaa cagtgtttct atcgttgaaa tccaaatttt
    44041 taggt
//
LOCUS       DAZUMV010000009        43189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000009 DAZUMV010000000
VERSION     DAZUMV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.009"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..718
                     /locus_tag="ACG8FK_000463"
     CDS             122..718
                     /locus_tag="ACG8FK_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFS5096101.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            699..1145
                     /locus_tag="ACG8FK_000464"
     CDS             699..1145
                     /locus_tag="ACG8FK_000464"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5096102.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1488..1811
                     /gene="relB"
                     /locus_tag="ACG8FK_000465"
     CDS             1488..1811
                     /gene="relB"
                     /locus_tag="ACG8FK_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS5096103.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            2131..2358
                     /locus_tag="ACG8FK_000466"
     CDS             2131..2358
                     /locus_tag="ACG8FK_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS5096104.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2374..3540
                     /gene="hemW"
                     /locus_tag="ACG8FK_000467"
     CDS             2374..3540
                     /gene="hemW"
                     /locus_tag="ACG8FK_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS5096105.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3775..4818
                     /gene="hrcA"
                     /locus_tag="ACG8FK_000468"
     CDS             3775..4818
                     /gene="hrcA"
                     /locus_tag="ACG8FK_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS5096106.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4912..5475
                     /gene="grpE"
                     /locus_tag="ACG8FK_000469"
     CDS             4912..5475
                     /gene="grpE"
                     /locus_tag="ACG8FK_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS5096107.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5526..7355
                     /gene="dnaK"
                     /locus_tag="ACG8FK_000470"
     CDS             5526..7355
                     /gene="dnaK"
                     /locus_tag="ACG8FK_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS5096108.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7506..8672
                     /gene="dnaJ"
                     /locus_tag="ACG8FK_000471"
     CDS             7506..8672
                     /gene="dnaJ"
                     /locus_tag="ACG8FK_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS5096109.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(9040..9777)
                     /locus_tag="ACG8FK_000472"
     CDS             complement(9040..9777)
                     /locus_tag="ACG8FK_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5096110.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9779..11281)
                     /locus_tag="ACG8FK_000473"
     CDS             complement(9779..11281)
                     /locus_tag="ACG8FK_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS5096111.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11540..13534
                     /gene="uvrB"
                     /locus_tag="ACG8FK_000474"
     CDS             11540..13534
                     /gene="uvrB"
                     /locus_tag="ACG8FK_000474"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS5096112.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13544..16360
                     /gene="uvrA"
                     /locus_tag="ACG8FK_000475"
     CDS             13544..16360
                     /gene="uvrA"
                     /locus_tag="ACG8FK_000475"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS5096113.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16623..17888
                     /locus_tag="ACG8FK_000476"
     CDS             16623..17888
                     /locus_tag="ACG8FK_000476"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS5096114.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17892..18623
                     /locus_tag="ACG8FK_000477"
     CDS             17892..18623
                     /locus_tag="ACG8FK_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS5096115.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            18758..19642
                     /gene="rapZ"
                     /locus_tag="ACG8FK_000478"
     CDS             18758..19642
                     /gene="rapZ"
                     /locus_tag="ACG8FK_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS5096116.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19639..20637
                     /gene="yvcK"
                     /locus_tag="ACG8FK_000479"
     CDS             19639..20637
                     /gene="yvcK"
                     /locus_tag="ACG8FK_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS5096117.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20671..21606
                     /gene="whiA"
                     /locus_tag="ACG8FK_000480"
     CDS             20671..21606
                     /gene="whiA"
                     /locus_tag="ACG8FK_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS5096118.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            22174..22830
                     /locus_tag="ACG8FK_000481"
     CDS             22174..22830
                     /locus_tag="ACG8FK_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096119.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            23009..23574
                     /locus_tag="ACG8FK_000482"
                     /pseudo
     CDS             23009..23574
                     /locus_tag="ACG8FK_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24273..24794)
                     /locus_tag="ACG8FK_000483"
     CDS             complement(24273..24794)
                     /locus_tag="ACG8FK_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS5096120.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            24992..28672
                     /gene="nifJ"
                     /locus_tag="ACG8FK_000484"
     CDS             24992..28672
                     /gene="nifJ"
                     /locus_tag="ACG8FK_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS5096121.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            28861..29748
                     /gene="cdaA"
                     /locus_tag="ACG8FK_000485"
     CDS             28861..29748
                     /gene="cdaA"
                     /locus_tag="ACG8FK_000485"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS5096122.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            29745..30914
                     /locus_tag="ACG8FK_000486"
     CDS             29745..30914
                     /locus_tag="ACG8FK_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HFS5096123.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYEFDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            30938..32293
                     /gene="glmM"
                     /locus_tag="ACG8FK_000487"
     CDS             30938..32293
                     /gene="glmM"
                     /locus_tag="ACG8FK_000487"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS5096124.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(32365..34170)
                     /gene="glmS"
                     /locus_tag="ACG8FK_000488"
     CDS             complement(32365..34170)
                     /gene="glmS"
                     /locus_tag="ACG8FK_000488"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS5096125.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            34580..34870
                     /locus_tag="ACG8FK_000489"
     CDS             34580..34870
                     /locus_tag="ACG8FK_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096126.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            34883..35908
                     /gene="sppA"
                     /locus_tag="ACG8FK_000490"
     CDS             34883..35908
                     /gene="sppA"
                     /locus_tag="ACG8FK_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5096127.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            35911..36570
                     /locus_tag="ACG8FK_000491"
     CDS             35911..36570
                     /locus_tag="ACG8FK_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5096128.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            36727..37659
                     /locus_tag="ACG8FK_000492"
     CDS             36727..37659
                     /locus_tag="ACG8FK_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096129.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            37813..39102
                     /gene="tig"
                     /locus_tag="ACG8FK_000493"
     CDS             37813..39102
                     /gene="tig"
                     /locus_tag="ACG8FK_000493"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS5096130.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            39349..40599
                     /gene="clpX"
                     /locus_tag="ACG8FK_000494"
     CDS             39349..40599
                     /gene="clpX"
                     /locus_tag="ACG8FK_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS5096131.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            40682..41269
                     /gene="yihA"
                     /locus_tag="ACG8FK_000495"
     CDS             40682..41269
                     /gene="yihA"
                     /locus_tag="ACG8FK_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS5096132.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(41343..41498)
                     /locus_tag="ACG8FK_000496"
     CDS             complement(41343..41498)
                     /locus_tag="ACG8FK_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS5096133.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            41634..42962
                     /locus_tag="ACG8FK_000497"
     CDS             41634..42962
                     /locus_tag="ACG8FK_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096134.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42973..43182
                     /locus_tag="ACG8FK_000498"
     CDS             42973..43182
                     /locus_tag="ACG8FK_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096135.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT        14314 a         7371 c         9325 g        12179 t
ORIGIN      
        1 tagatgaggt ataataatga tatttttata tattacagat aacttgacac aaaatgagtt
       61 tttttcgatt tttcaagtta tctaatgcaa ttatattgat atcattagga gctgcattgt
      121 tatgaatcca acattgatta ctaagaaaga attactaaaa aaattagata tttctactgg
      181 tgtattagct aatttaatta gaaatggcat gccaaaagag ggagaaatgt ttaatcttga
      241 taagattatt acatggcgag aaaattggtc taaaaacata ttaggtgagt tagaagttgg
      301 tagagtatat actaacaaag aaatttcaga gaagtttaaa tgttccaaac agggtggaat
      361 gagaagatct catcaaacta atactcttgt actgtttagt gatcaaacag gaagtaatgt
      421 ttataaagat aagtggttaa atggtatttt acaatatacc ggtatggggc taaaagggga
      481 tcaagtattg gataagaatc aaaataaagt tttagccaat tcaaaaagta attttgtaaa
      541 gattcattta tttgaaacat ttaaaccaaa agaacacaca tatctgggag aagtatattt
      601 agcaggacaa atatatactg ttaatgaaaa agatagttcg ggcaacagca gaaaagtata
      661 taaatttcca ttagctttaa taaatcaaga gcaattgatt gaagataaag atatataacc
      721 aggaagaaaa tcaaactaga catattcgta atttatctaa tgctaaatta gaggaagaag
      781 caagaaaagt tagtaattat aataaaaaaa tggctgcaaa atatgttaac gaagaggagt
      841 tttgcttctc aaaaacaaaa atatataaac gtaatccctt cgtatctgaa tatgttaaac
      901 gacttgcaaa aggtatttgt caactttgcc aagaaaaagg gccatttata aaagacggtg
      961 taccttattt acattgtcat catatagaat atttgtcgca aggtggaaaa gatgtgattg
     1021 aaaactgtat cgcgttatgt cccaattgtc atgctagaat tcatgaactt gagttacctg
     1081 acgataaaga aaaattgctg aagatcgttg aaaaaagaga aaatagcttt tctaaaatac
     1141 cgtaatttat ttttatagga caaataagct aatgtgagct atgccttcta tataagacta
     1201 agttctatta taagtaacga agtaattctc gttccttcct gtagtaatat tttttaaata
     1261 acctataggc ttcttcaaaa gtacatcctc cagtaaattc taagtactgg aggattcttt
     1321 ttctagtgtt gctttactca tgaaaaagta gttggctact tcatcaaaat ttctgaactt
     1381 ttttaatgta taataaagag ctcgatcatt tactacaagg atgctagctc ctacattttc
     1441 acagtttttt agcaagtaaa tttttctaaa atcacatcag gaaatgcatg agattcttta
     1501 agagaggcaa ttatagggct atgattacgg ttcgtgtaag tgacgccaaa aaagaatggc
     1561 tttcttatat ggctgaattt tatgggatta ctttatctga acttctaaaa aactattcga
     1621 tggaacaact agaagatgaa tacaatcgtc aaacggctga aattgcccat aaacattgga
     1681 taagaagtgg gaaaacaaca gtattcatgg aagaatcctt aatgaatttg gtgggctgga
     1741 atgacctgcc gtttaaaatt cacgcccgaa attcagaagc agctctgatt ggcaaatacg
     1801 tgtattggta gagatagaag ataatcaatt cgttgttatc gctattcaag tagggcaccg
     1861 aagaaatgtc tatgattaag agactgttta tttttacaaa aaaataataa ttggaatcta
     1921 gttaaaactc ttgaagtgag agatttgact agattttttt atgaataaaa taaggacaaa
     1981 attgtttagg aagaatcatg agtgtaaaaa aatcatccaa gaagtcgatt cttggatttt
     2041 ttagtggatt tgttcgccaa atacgtttag tatgcgtata tgagaggttg taagcaaagc
     2101 gttgtggtct gagcacttaa ggaggaatct gtgaaaatag cttttcatat ttttgcagat
     2161 tttgacttaa tgccgaaggt cagctttgcg attaccgttt atcgagaggc tgtaaaagca
     2221 ccgtgtagct ccgatgacaa taagcaagca tttttagagc taggtttcct tgattctaaa
     2281 tatgcggctt atttcaatga gttggtgctt gaacgcgatt ttaagaaggt cgaaaacagg
     2341 cacgactttt cgacgtaagg agaaagaatt tcaatgatag aaacaataaa tcccgcagaa
     2401 aacaaggttt ctgcgtacat tcatatccca ttttgcgagc atatctgcta ttactgtgac
     2461 tttaataaag tttttttaga agggcagcca gtagatgagt atgtggaaat gctgctgaat
     2521 gaaatgagat taatgctgga aagtcatcct acagcgaatt tggaaacact ttatgtagga
     2581 ggcggaacac caacttcttt atctgctaaa caacttgatc gactattatc aggtgctaga
     2641 gagatcctgc cttttcaaaa tggtaatgag tttactgtag aagcgaatcc tggtgatttg
     2701 acccgtgaaa aacttcaagt gatgaaaaac tatggcgtca atcgtttatc catgggagtg
     2761 cagacatttg acaatcgctt attgaaaaaa attggacgta aacatactgc agaagatgtg
     2821 tatcaaacaa tgcaattttt agaagcagaa aattttacca atgtaagcat cgatttgatt
     2881 tatgcattac cgggacaaac actagaagga aatcgggata ctttggaaaa agctttggct
     2941 ttggatctgc ctcactattc tttgtactca ttgatcctcg aaaacaaaac aatgttcatg
     3001 aactgggtac gacaaggaag actcgaactg cctgatcagg aaacggaaac cagaatgttt
     3061 gaagagacga ttgaagcaat ggaaaagcat ggacgtcacc aatatgaaat aagtaacttt
     3121 gggctctccg gacatgaatc tcaacataac ctcatgtact ggaacaatga ccactatttt
     3181 gggtttggag ctggtgctag cggttatctt ggagatattc gttaccgcaa caaaggacca
     3241 atccaacact atttacgccc attacgagca aatgaacgcc cggtattgga agaagaaaaa
     3301 ttgacacgaa aaaatcaaat agaagaagaa atgttcttag gactaagaaa aaaaacaggg
     3361 attactaaaa aacatttcta tgatcgctat caaaaaacaa ttgaatcggt gtacggtgat
     3421 gtgctaaggg aattagagca agaagggtta cttgttaatg aagaagaccg tgtttatttg
     3481 acaccaagag ggactttttt ggggaacaat gtgtttgagc gctttttatt tgaaaaatag
     3541 tttgaagaat atttgttttt tatcgaaact tgaaaagaaa aagtttagaa agtttgacaa
     3601 tgaggtattg aagtattgat ataactggat ttacatcaat tatttagaat tagcactctt
     3661 gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag gagaagggct tcttgctata
     3721 tttataagtg aattagcact tagaatatat gagtgctaat gagaggtgag gaagatgtta
     3781 acaaagcgac aaaacgacat tttacgtctg atcatccaac actatacgag tagtggcgtg
     3841 cccgttggtt ccaaaacatt aatgtctgag ggagtcaagg ccagctcagc aacgattcgc
     3901 aatgatatga aagcattaga agatgaagga ttgctgttga agactcactc ttcttctggg
     3961 cggattccat ctgctttagg ctatcgttat tacgtggatc atttgttaaa acctgctcgc
     4021 gtagcagaag atgatttgct gctgattcgt cagtctctag gcagagaatt tcatgaaatc
     4081 aatgagatta ttaagcaatc tgctgagata ttatctgagc tgacaagcta tacgactttt
     4141 tcattgggac ctgaaataaa agaccgccga ctgactggct ttcgtatcgt acccttgaat
     4201 aatcgacaga ttattgctat cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc
     4261 actcttcccg aatatatggg aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac
     4321 cgtctagtcg gagatcctct gatgacggtg taccataaac ttcgtacgga gattccgatg
     4381 attcttcata aatatttcca gacgacagaa gggatgtctc agttatttga caccgttctt
     4441 ggtcatgttt ttgaagaaaa ggtttatgtc agtggacaaa tgaatctctt ggattatgag
     4501 ccccgtcaag atcttgatca gttcaaatcg atgttttctt ttatgaaaga ctcagacgga
     4561 cttacacaaa tgatcactcc gatggatagc aatattcata tcaagattgg tgctgaatta
     4621 ggcaatgatt tattgcagaa tatgagtatg attcaagcaa gttacgagat agatggtcat
     4681 ggaagaggta caatagcctt gttagggcct actagtatgc cttattctcg gatgttcggt
     4741 ctagtcgatg tttatcgaag agaactagca gctaaacttg ctgattacta ccgctcgctt
     4801 gacttgtcaa actcgtaatg tgaataacga agctatttgc agctgatcgt tctctctttc
     4861 ttagtaagag caatcaaaca gctgactgaa taatgaaagg aagactgtat cgtgaaagaa
     4921 aatcaagaag aactagataa agaaatgaca gatgctcagc cagaaccgga aatcgatgtc
     4981 gaagctgcag aagattcggg tatttcagaa gctgaagcgg aagaattcga aacagcgaaa
     5041 ttaaaagctg agttagaaga aatggaagat aaatacttgc gggctcgtgc tgaaatcgca
     5101 aatatggcaa atcgaggaaa aaatgagcgt gagcaattac aaaaataccg ttcacaagat
     5161 ttagccaaaa aattacttcc atcaattgac aacctggaac gtgcattagc cacagaagtg
     5221 tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt
     5281 aacgcactgg aagaagaagg aattgaaaaa atccctgcta aaggagaagc ctttgatccc
     5341 aatcttcatc aagcagtaca aactgttcca gcgactgaag atacaccagc cgatacgatt
     5401 gtagaagtac tacaagaagg ctacaaatta catgatcgcg tgttaagacc aactatggtc
     5461 attgttgcac aataattgtg cggatataaa acttacactt tcaattaaag aaggagacgt
     5521 taattatgag taaaattatc ggtattgact taggtacgac taactctgca gtagcagtat
     5581 tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg aaaccgtaca acaccttctg
     5641 ttgtatcatt caaaaatgga gaaatccaag taggggaagt tgcaaaacgt caagctgtga
     5701 caaacccaaa tacgatttca tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag
     5761 atgtagaagg taaatcatat acaccacaag aaatctcagc aatgatcctt caatatatca
     5821 aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag
     5881 cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc tggtaagatc gctggtctag
     5941 aagtagaacg tatcgtcaat gaaccaactg cagctgctct tgcctatggt ttagataaaa
     6001 cagatcgcga cgaaaaaatc ttagtattcg accttggtgg cggtactttt gacgtatcta
     6061 tccttgaact aggagacggt gtctttgatg tattgtcaac agctggcgac aaccatctag
     6121 gtggggacga ctttgataac aaaatcatcg actacatggt agcagaattc aaaaaagaaa
     6181 acagcattga cttgtctcaa gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa
     6241 aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat cagcttgcca tttatcacag
     6301 caggagaatc tggaccattg catttggaaa tgacattgac acgtgcaaaa tttgatgaat
     6361 taacagctga cttagtagaa cgtacaaaag taccagtacg tcaagcgtta aaagatgcag
     6421 gtctgtcaca atcagaaatc gacgaagtta ttttagtcgg tggatctact cgtatccctg
     6481 cagttgtaga agcagtacgt aaagaaacag gaaaagaacc aaacaaatca gttaaccctg
     6541 atgaagtagt tgctatgggt gctgctatcc aaggtggcgt gatcactggt gatgtgaaag
     6601 atgttgtttt acttgacgtt acaccattgt cattaggtat cgaaacaatg ggtggagtgt
     6661 tcactaaatt gattgaccgc aatacaacga ttccaacaag taaatcacaa gtattctcaa
     6721 ctgcggcaga taatcaacca gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg
     6781 ctgctgacaa caaaactcta ggaagattcc aattgacaga tattccagca gctcctcgtg
     6841 gtattccaca aatcgaagta acattcgata ttgataaaaa cgggattgta aatgtatctg
     6901 cgaaagatct aggtacacaa aaagaacaaa aaatcacgat caaatcttct tcaggtctaa
     6961 cagacgaaga aatcgaacgt atggtgaaag atgcagaagc aaacgctgaa gcagataaag
     7021 ctcgtaaaga ggaagttgac ctacgtaatg atgtagacgc attgctattc tctgtggaca
     7081 aaacattgaa agaattagaa ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag
     7141 ctcgtgacga attaaaagcc gctgttgaag caaacaacat cgaggaaatg aaaactaaac
     7201 gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa actatacgaa caagctgcac
     7261 aacaacaagc tcaagaaaat ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg
     7321 ctgattttga agaagtcgat ggcgacgata aataatcagt tcactgaatt tcgagagaag
     7381 gccaaagttg ctactttggc tttttctttt gaaaaatagt ttgaagtatc ggtttttgtt
     7441 tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg atgagaacca ataggaggaa
     7501 agcctatggc aacaaaacgt gattattatg aagtcttagg gctctcaaaa ggagcatcag
     7561 aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa ataccatcct gacatcaata
     7621 aagaacctga tgcggaagaa aaattcaaag aagtatcaga agcatatgag attttaagtg
     7681 acccgcaaaa acgcgctgca tacgaccaat acgggcatgc aggcacagat ccaaattacg
     7741 gtgctggtgg agctggtggc ggatttggtg gattcggcgg cttctctggt ggcggcttcg
     7801 gtggttttga agatatcttc gactctttct ttggaggagg cggtcggtca gtcgatccaa
     7861 atgcgcctcg tcaaggtgct gatttgcaat atacggtcga cttgtctttt gaagaagcga
     7921 tttttggtgt agaaaaagaa atcaagtata atcgggaaga aatctgtcat acttgtggtg
     7981 gaaacggtgc aaaaccaggc acacaaccaa ctacttgtca taaatgtcat ggtgcaggta
     8041 cgatcaatgt agaacgccaa acgccacttg gacgagtgat gagccgtcaa acttgtgatg
     8101 tttgtcatgg cacagggaaa gaaatcaaag aaccttgtcc aacctgtcat ggctcaggac
     8161 atgagaagaa agcacattca gttaaagtga atgtaccagc tggtgtagaa gaaggacaac
     8221 aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg cggaccattt ggtgatttgt
     8281 atgtggtttt tcgtgtagaa gaaagtgata tctttgatcg tgaaggttct gaaatttact
     8341 atgaattgcc tttaagtttt gtacaagcag ctttggggga tgaagtccaa gtaccaactg
     8401 tccatggaga tgtgaaattg aagatccctg ctggaacaca gacaggtacg aacttccgtt
     8461 tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac aggcgaccaa catgtcaaag
     8521 taaaactgat cacaccgaaa aatctaaatg aacaacaaag agaagcatta cgtgcattcg
     8581 ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga cggtttcttc gataagttta
     8641 aagatgcttt tggcgggaaa aagcggaagt agtttttcta gtaaaaatat aaagtagact
     8701 tgcccaaatt ttctatttct agtaaaaacg ctaagtcatt aataattaaa aaccagacga
     8761 acaatcatac gttcgcctgg tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca
     8821 gctttagaat aagataatca tcatagtttc tgaaggtggt atcgactatg tacttgatat
     8881 cgtcaatgaa ccgatggtac tttcttatgc gatggaacag gtagagcgat tgcttgtttg
     8941 tgcaggagaa caagcgatgc ggctggtaca gttaagtgaa aaaacaagaa actaaaaatg
     9001 cccggtcgtc atgaagcgac cgggcatttt tggtttttat taaatattta atactttatt
     9061 caagaaatct tttgtccgtt cattttgcgg attttcaaag atttgttgag gcgttccgtc
     9121 ttctaaaaag tttccgtcgt caatgaacat gacacgattg gccacttctt tagcgaagcc
     9181 catttcgtgc gtaacgataa ccatcgtcat accttgttct gctaactttt tcatgacgtt
     9241 caagacatca ccgaccattt cggggtcgag tgcagatgta ggctcatcaa aaagcatgat
     9301 gtcagggttc atcgctagag cacgagcaat agccacacgc tgcttttgtc caccggaaag
     9361 catttcagga tacatatctt ttttatcttc tagaccaact gtctgcagca atttgattgc
     9421 ttttttctct gcttcttctc tagattcttt cttcaggtcc atcggtgcta aaatgatgtt
     9481 ttccaaaata gaaagatgag ggaataggtt aaagtgctgg aataccattc cgatatgttg
     9541 tctcaccaag ttgatatctg tgcttttatc catcaagtgg gcgccgtcaa taataatttc
     9601 accgtcagaa ggttcttcta aacgattcaa gcaacgcaaa aatgtacttt ttcctgaacc
     9661 tgaagggcca atgatacaga cgacatctcc ttcgttgatg gacacattga tgtcgtttaa
     9721 tactgtgtta tcgccgtatt ttttgactaa atgctcaact aagatttttt ctgccatctt
     9781 acttcacctt cttttctaaa acttttgcta attttgtcaa tgctgtgatc aaaatcaagt
     9841 acatgatcgc aatgatcaag taaacatacg tactttgtgt tgtacgtgca acaatgatct
     9901 taccagtttg cagcaattcg actacaccga ttgcggaaat gatcgttgtg tcttttaatg
     9961 agataacgaa ctgattgacg aatgatggga tcatgatgcg tatcgcttgc ggcaaaatga
    10021 ttttttgcat tgtccgatta taagaaagac ctaaacttct ggaagcttcc atttgtccga
    10081 caggaacggc gttgatcccg cctcgaacaa tctctgcgat ataagcactt gcgttaagag
    10141 tcaaggtgat aacaccagca aggaagtcag gaatcgtaaa tccaacgata ccaggcaacc
    10201 caaagaaaat aaagaacgca agaaccatca ttggaattcc tcggatgata tcgacataaa
    10261 tactagcaat tgtgcgaaga gcgcgtattg gtgctacact gaataatcca aaaatcacac
    10321 cgattactat agccaaagca aaggagatca aagttaggag aattgttctc caaagtcctt
    10381 tcaacaatgc ttgatagttg ttttggataa gtccaagaaa tgttgattca tcagcagtgg
    10441 aagactcact ggaagtttct gaaccatccg ctacatattc tttaataatc ttatcgtatt
    10501 cgcctgttct ttttaattct ttcaatcctt cattgaacat attaagaagc tcaggatttt
    10561 gtcctttttt aacggcaaat gcatattttc cgccagattc ttttttgatc ggtgtttcca
    10621 attcttgtcc ttgtttgatc gcataaccga ttaccggata atcatccatc attgcatcga
    10681 ttgcgccaac ttttaacccg tcgtacaatt gatcagcagc atcaaaatat ttgatagaat
    10741 agccatattc agattcatgg tcgcttaaaa attcagcact ttcggtacca acttttgctc
    10801 cgactgtttt tcctttcaaa tcttcataag acttgatcga agtatctcct tttttgacag
    10861 ctaattggat cccgctatca aagtaagaat cagaaaaatc atagcttttc tttcgttcat
    10921 ctgttacagt cattccggca atcattccgt ctgcttgtcc tgattccaaa gcttgcaaag
    10981 cagcagagaa accacggtag tccattcgga tgttaaatcc ttgcagtttg gcgatgtttt
    11041 ccatcaattt tacatcgatg ccttcgtact cgccctttgc gttttgaaat tcaaatggag
    11101 caaaagcaga gtcactggca attacgtata cgtctttttt gggctctatt ttttgcattg
    11161 tctcgcttgt gcttgactct gccgttgtag tgttgtcagc ttgtgcaaca tgtccaaacg
    11221 caagtgtcac aagtcctgtc attaagaaaa caaatgagtg aaataaatgc tttcttttca
    11281 taatcaacct cctaaatttc caatatttta ttattatagg cgaaaagtgc tgaatcagac
    11341 aagactgtaa gctcgaaaaa atgaaaaaaa tgcgagtttc taacatttta tgttataaaa
    11401 cgtaacatgc ataataagat aaaaaagtgg cgaaagtatg ttcggtgtgg tataattttt
    11461 tagtaaggaa tgcctttgct ttccttttct ttttattggt cgtaaaatga aaaggaatga
    11521 atgaaaagga ggacgaagaa tgattgagag agatacctcc cgacactttg agcttgtctc
    11581 gaaataccaa ccggctggtg atcagcctga agcgattaac caattagtgg atggcgtagt
    11641 cggcgggaaa aaagcacaaa tcttattagg tgcgactgga acagggaaaa catatacgat
    11701 ttccaatctt attgagaaag tgaataaacc aacactaatt attgcccata acaaaacatt
    11761 ggctggtcag ctgtacgggg aattcaagga atttttccct aacaatgctg ttgaatattt
    11821 tgtttcttat tatgattatt atcagcctga agcttatgtg ccttcaagcg atacgtatat
    11881 cgaaaaagat tcaagtgtaa atgatgagat cgacaaatta cgacattcgg ctactagttc
    11941 attattagaa agaaatgatg tgatcgtcat tgcatccgtt tcatgtattt ttggattagg
    12001 ttcgcctttt gagtaccaaa aacaagtagt ttcgattcga cagggagcag aactagatcg
    12061 caatcagttg attcgtgact tagtcagtat ccaattcgaa agaaatgata tagattttca
    12121 gcgcggtcgt tttcgagtgc gcggtgatgt cgtggagatt tttccggctt caagagatga
    12181 acgtgcctta cgtgtggagt tttttggaga tgaggtcgaa cgtatccgag aagtcaatgc
    12241 actgacaggt gaagttttag gagaaacgga acacgtggca atcttcccgg cgactcactt
    12301 tgtgacgaat gatgaacata tggagcatgc cgtagcgaat atcaaagcgg aattagaaca
    12361 gcgtttaact gtcttgcgaa atgaaaacaa acttctggaa gctcaacgtt tagaacagcg
    12421 aaccaattac gatattgaaa tgatgttaga aatgggctac acgtcgggaa ttgaaaatta
    12481 ttctcggcat atggacggac gtaaagaagg tgaaccgcct tatacgctat tggatttctt
    12541 tcctgaagat tttttgatcg tggcggacga atcgcacgta accatgcctc aagtcagagg
    12601 aatgtataat ggtgaccgag cgagaaaaca gatgctagta gattacggtt tccgtttgcc
    12661 atctgcttta gacaatcggc cacttcggtt agaagagttt gaaaaacatg tcaatcagat
    12721 catttatgtc tctgcgacgc caggaccata tgaacatgaa caaacagata ccgttatcca
    12781 acaaatcatc cgtccgacag ggctgcttga cccagtgatc gaagttcgtc caatcatggg
    12841 acaaatcgat gatctagtag gcgaaataaa tgaacgagtc gaaaaagacc agcgggtgtt
    12901 tgtaacgaca ttgacgaaaa aaatggcaga agacctcaca gattatttca aagaattagg
    12961 gatcaaagtc aaatatttgc atagtgatat caaaacgctg gaacgaacag aaatcattcg
    13021 agatcttcga ttaggtgaat ttgatgtgtt agttggaatc aatttgcttc gtgaaggatt
    13081 agacgtacct gaagtttccc ttgttgctat cttggatgcg gataaagaag gctttttacg
    13141 tagtgaacgt tccttagtcc aaacgattgg acgagctgct cggaatgaag aaggtaaagt
    13201 aatcatgtac gcagacaaag taacagattc tatgcgtcta gcgatggatg aaacatcaag
    13261 acgccgtacg attcagcaga agtacaatga agaacatggg atcgttccaa aaacgatcat
    13321 taaagaaatc cgagatttga tttctattac gaaagaatcg gaagatgata caaaagaagc
    13381 ggtacaagtc tcgtatgaag aaatgacgaa agaagaaaaa gatacgttat tgatgaaatt
    13441 agaaaaagaa atgaaaaatg cggcgaaagc attggatttt gaaacagccg ctaatgtgcg
    13501 agatatgatt ttagaattaa aagcttcaaa gtaggaggac aacatggcta acgataaaat
    13561 tacgattcac ggtgcacgtg cccataactt gaaaaatata gatgtaacga ttccacgaga
    13621 taaattcgtg gtcgttacag gtttgtccgg ttctggaaaa agttccctag catttgatac
    13681 gctgtacgct gaagggcagc ggagatacgt agagagcctt tctgcttatg ctcgtcagtt
    13741 tttaggacaa atggataagc cggacgtaga tagcatcgac ggattgagtc cggcgatttc
    13801 tattgatcag aaaacaacaa gtaaaaatcc tcggtcgaca gtaggtactg tgacggaaat
    13861 caatgactat ttacgcttgt tgtttgcacg tgtaggacat ccgatttgtc ccaacgatca
    13921 tatcgagatt accagtcaat cagtagatca aatggcagat caagtaatgg ctttgcctga
    13981 gcgcacaaag atccaagtgt tggcaccgat cgtggtaaag aaaaaaggac aacataaaaa
    14041 aatattcgaa cgaatccaaa aagagggata cgtccgcgtt cgagtcgatg gtgagatcta
    14101 tgatttaagt gaagtgcctg agctggagaa aaacaaaaaa catgatatcg cgatcgtcat
    14161 cgaccggatc attgtgaaag aagggattcg ttctcgtcta tttgattctt tagaggcggc
    14221 tctgcgattg gcagacggct atgctctggt tgacgtgatc ggtgaagaag aaatgttgtt
    14281 tagcgaacac tatgcctgtc cttattgcgg atttactgtt ggcgaactag aaccaagact
    14341 gttctcattc aatgcgcctt ttggagcttg tccagactgt gatggtttag gtgtcaaatt
    14401 agaagtcgat caagatttag tgattccaga tagaacgaag acacttagtc aaggtgctat
    14461 cgttccatgg aatccaatca gctctcagta ttatcctcaa atgttagcgc aggcttgtga
    14521 aagtttcggt atcgatttag atacaccatt cgaagaatta ccggaagagc atcaggatat
    14581 catcttgaat gggacagatg atcttttcca tttccattat gaaaatgatt ttggtggcgt
    14641 tcgcgatgta gaaacgactt tcgaaggggt attgaagaat atcgagcgtc gttatcatga
    14701 tacgaacagt gatttcacgc gtgagcaaat gcgtctttat atgacagaat taacttgtca
    14761 gacatgtaaa ggttatcgat tgaatcccca agctctatct gttcaaatca atggaacgaa
    14821 tatcgggcaa acaaatgagt tgtctattca aaaagcagta gatttcttct caaacttaac
    14881 gttatctgaa caagagaaag taattgccaa accgatttta aaagaagtaa atgacagact
    14941 ttcattcttg gaaaatgtgg gactagacta tctaactttg agtcgcgctt ccggaacatt
    15001 atctggggga gaagcgcagc gaatcagatt ggctacacaa attggatcga atctttctgg
    15061 agttttgtat attttagatg aaccatcgat cggattgcat caacgcgaca acgatcgact
    15121 gattggctca ttaaagaaaa tgcgtgatct tggcaacacc ctcgttgtag tcgaacatga
    15181 tgaagatacg atgtgggcag cagattatct gattgacgtc ggacctgggg ccggagaaca
    15241 aggcggacaa atcgtggctg caggcactcc tgaagaagta gctaatgatg aaaattcttt
    15301 gacagggaaa tatctttcag ggaaaaaatc aatccctgta ccaaaagaac gccgtaaagg
    15361 taatgggaaa gcaatcaaga tcactggtgc ttcagaaaac aatctgaaaa atattagtgt
    15421 tgaatttcca ttaggagaat ttgtagcagt cacaggtgtg tctggttctg gaaagagtac
    15481 attggtcaat agtatcttaa agaaatcgtt agcgcaaaaa ttaaataaga attctgctaa
    15541 gccaggtaaa ttcaagacaa tttccggcta cgaaagtatc gaaaagatca tcgatatcga
    15601 tcaaagccca atcggccgga cgccgagaag taatccagcg acttatacaa gtgtatttga
    15661 tgatatccgt gggttatttg ctcaaacgaa cgaggcaaaa atgcggggtt ataagaaagg
    15721 gcgttttagt ttcaacgtaa aaggcggtcg ttgtgaagct tgtcgcgggg atggaattat
    15781 taagatcgaa atgcactttt tgcctgatgt ctatgttcct tgtgaagtat gtcatggcaa
    15841 acgatataac tctgaaacat tagaagtgca ttacaaagga aaaagcattg ctgatatttt
    15901 ggaaatgaca gtagaagatg ctgtagaatt cttcaagcac attccaaaga ttcatcgcaa
    15961 actgcaaacg attgttgatg ttggcttagg ttatgtgacg atggggcaac cagcaacgac
    16021 attgtccggt ggtgaggcac aacggatgaa acttgccagt gaattgcaca aaatctctaa
    16081 tggaaagaat ttctatatac tagatgaacc aacgacagga cttcatagcg atgacatcgc
    16141 ccgcttgttg catgtattac aaagattagt agatgctggt aacacagttt tagtgattga
    16201 acacaatcta gatgtaatca aaacagcaga ttatatcatt gatttaggac cagaaggtgg
    16261 agaaggtgga ggaacgatcc ttgcgactgg aacaccagaa gaaatcatta acgtaaaaga
    16321 aagttataca ggtcactatt tgaaaaaaat aatggtataa agaggataga tagctgagat
    16381 agttaaaaaa acataaaaaa atgaattttt gcttgtttta ttaatatcag tgttctagaa
    16441 tcattacatg gtagttcttt ctacactgat tggtatagac aaaaacttcc tataagttca
    16501 atccgttcaa aaaagaattg agcaatcgga ggagtttcct ttgattgctt ttttctttat
    16561 ggtatgatat tccttgtgta gttggaaaag aaaaacaaga gattatgagt aagtgagaga
    16621 taatgatgaa cagtattgaa aatgaagaat ttatccctat attattagga agcgacatga
    16681 acgtttatgg tatggcacgt tcttttaatg aagcatacgg caaaatctgc caagcatatg
    16741 cttcggatca gttagcccct acccgctaca gtaaaattgt caatgtagaa gttattccag
    16801 gatttgataa agaccctgtg tttatcgaga cgatgcttcg tttggcaaaa gaaagatatt
    16861 ctgataagag taaaaaatat cttttgattg cttgtggtga tggatatgca gaattgatct
    16921 cccaacataa acaagagctg tcagaatatt ttatctgtcc atatattgat tattctttat
    16981 ttgaaagact gatcaataaa gtaagctttt atgaagtttg tgaagaatat gatcttccgt
    17041 atcctaaaac attgatcgtc agagaagaaa tgttagtaaa tggacatttg gaacaggaat
    17101 tgccttttga attcccagtt gctttgaaac ctgcaaacag tgtggagtat ctttctgtcc
    17161 aatttgaagg aagaaaaaaa gcttttattt tagagactag agaagagttc gatttaattt
    17221 taggccggat atacgaagcc ggttataaaa gtgaaatgat tgtacaagat tttatcccgg
    17281 gtgatgatag taatatgcgt gtgctaaatg cgtatgtaga tgaagatcat caagttcgga
    17341 tgatgtgtct tggtcatcca ctgttagaag atcctacacc agcatcgatt ggtaattacg
    17401 ttgtgatcat gccagattat aacgaaaaaa tctatcaaac cattaaggca tttttggaaa
    17461 aaatcgaata tacaggcttt gccaatttcg atatgaagta tgatcctaga gatggagaat
    17521 ataaattatt tgaaatcaat ttgagacaag gtcgtagcag tttctttgtg acattgaacg
    17581 gcttgaatct ggctcgtttc gtcacagaag atcgagtgtt taacaaaccc tttgttgaaa
    17641 cgacctatgg aacgaatcaa tcagataaag caagattatg gatgggtgtg cccaaaaaaa
    17701 tctttttaga atatgctcgg gaaaatgaag acaaaaaact agctgagcaa atgatcaaag
    17761 aaaatcgtta cggtactact gtgttttatg aaaaagaccg gtcaatcaag cgctggttgt
    17821 tgatgaaata tatgttccat aactatattc caagattcaa aaagtatttt catgtgaaag
    17881 aaggctagtt catggagaat tttttcagta ttttaggcgg aatgggcacg atggcgacag
    17941 aaagttttgt tcgcttgatc aaccaccgaa cgaaagctac aaaagatcaa gaatatttga
    18001 attatgtctt attcaatcat gcaacagttc ctgatcgtac agcttatatt ttagatcgat
    18061 cagaagaaaa tccaatgccg tttctgctgg atgatattga gaaacaaaat ttattgcagc
    18121 cgaacttcat tgtgttgact tgtaacaccg cacactactt ttttgaagaa ttacaagcgg
    18181 caacggatat tcctatcctt catatgccaa gagaagcagc aaatgaactt gtccgtcagc
    18241 atacgacagg gagagtagcg attctaggaa cagaaggaag tatgaaagct ggaatctatg
    18301 aacgcgaagt caaaaatctt ggattcgaaa cagtgattcc tgatactgtt cttcaagaaa
    18361 aaatcaatta tctgatctat catgagatca aggaatcgga ttatttgaat caagagctat
    18421 attacgaaat cttagaagaa gcggtagagc gcctaaactg tgaaaaagtt atcttaggtt
    18481 gcacagaatt gtcattgatg catgaatttg ctgaagataa ccattatcca gtgatcgatg
    18541 cccaatctat tttagcagat cggacgattg aacgagcgct agctgaaaga agcaaggcat
    18601 tggatacagc atcggaaaag taaaaaaatt ggatttgggg caaatatcct aaaagatatt
    18661 tggtccaaat ctttttgtaa aagaaaaaga aaactggtaa tgatacgccc ggaatgatat
    18721 aattttacta tgcacaatgt gagaggagtt cgtaattatg acagataatt tgcaattagt
    18781 catcataaca ggtatgagcg gtgcagggaa aacagtagct atccaaagtt ttgaagatat
    18841 gggctatttt tgtatcgata atatgccgcc tcgattgatt ccaaaattct gggaattaat
    18901 caaagaatca ggcaaagtca caaaaatggc tttaggagtg gatttacgtt ctcgttcctt
    18961 ctttgaagaa atccaaaaca tgctgatcga aatcgaaaat acgaacttca tcgacacttc
    19021 tatcttattt ttagatgctt cagacgaaga actagtggct cgctacaaag aaacaagacg
    19081 agcacatcca atggcgatgg acggtttgat cactgaaggg atacgtaaag aacgggcgat
    19141 actagacgac ttgaaagccc aagcttccgt gatcattgat acgacgacat tgacaccacg
    19201 acaattacgt gaaaaaatca atgagtcatt caaatcaaag ggagaccaag gatttcggat
    19261 cgaatttgta tcatttggtt ttaaatacgg gttgccgatc gattcggata ttgtgatgga
    19321 tgtgcgtttt ttgcccaacc ctcattatat cccggaacta agaccattga ccggaaaaga
    19381 tcagccagtc tatgattatg tgatgtcctt tgatgaaacg caatcattct accataaatt
    19441 tttggatctt ctggaaactg tattgcctgg atacattaaa gaaggaaaaa gcagcttgac
    19501 agttgcaatc ggctgtacag gcggtcaaca ccgttctgtg gcattgactg aacgtttggc
    19561 aaatgcttta gggaaaaact ataaagtcaa tgtgacacat cgggacaagg ataaaaggaa
    19621 agagacggtg aaccggtcgt gaaaatgaaa acctaccgga taagaaaacc aaagatcgta
    19681 gtggtcggcg gtggcactgg tctgccagtt atcctgaaaa gtttgcgaaa tcaaggagca
    19741 gatatcacag cagtagtgac tgttgcagat gatgggggaa gcagcggtgc aatcagagaa
    19801 tcgatcgcta tggctcctcc tggtgacttg agaaatgtct tagtggcgtt atcggatatg
    19861 ccacaattct atgaagatat tttccaatac cgtttcaaaa aggaagatca gtttttagcc
    19921 aatcatacga tcggtaatct gatcattgcc gcagtttccg agatgcgggg aagcacttat
    19981 gaggcgattc aattattagc aaaaatgatg catgtagatg gacatgttta tccttcttca
    20041 gaaactcctt tgaccttgca tgctgtcttt aaagacggaa cgactgcagt aggagaatct
    20101 aaaatcgcga tcgaccgtaa aaccattgac cgtgtgtatg tctgcaacac aaatgataaa
    20161 agtgaagcca aagcggctcg aaaagtcgtt tcagcaatta tggacgccga catggtcgtt
    20221 ttgggacccg gaagtttatt caccagtatc cttccaaatt tagtgatacc agaaatcggc
    20281 gaagcaatgc tcaagacaac tgctgaaacc gtctatatct gtaatatcat gacccaaaaa
    20341 ggggaaacgg aacatttcac agatgccgat catatacgtg ttttacacaa gcatttgaat
    20401 cagcctttta tcgatacagt attggtcaat acggaaaaag tgcctgaaga ttatatggac
    20461 ccagagatct atgatgagta tctagtacaa gtcgcacacg acttcaacgg attgagaaat
    20521 gaaggatgtc gagtgatttc caccgacttt ttggaattga aagatggtgg tgtcttccac
    20581 gatggagaca aagtagtaga agaattgttc cgtttagttt tcggttcaaa atattaagca
    20641 atttattgtt ggagaaagga ggagaatagg atgtcatttg ctgcagatgt gaagaaagaa
    20701 ctaactggtt tggaagttca tcgagaacat gcgaaagcgg aattagctgc tttgctgcga
    20761 atgaacggct ctttaggtat tgtgaaccag cagtttattt tgaacgtcca aacagaaaat
    20821 gctgcaattg ccagacgtat ctattctctt ttgaaagatc attatcaggt acgaagtgag
    20881 ctactcgttc gtaaaaaaat gaaattaaaa aagaacaatg tctatatcgt ccggttgaag
    20941 caaggcacaa aagatgtgtt gatggatctt gatatcatgg atggtatgat gttcaatgga
    21001 cacgtttcgg acgagattat gggaaattcg caaaaaatgc gttcttactt acgtggtgct
    21061 tttatggcct caggttcagt caataatccg gaaacgagcc gttatcattt ggaaatattt
    21121 tctatttatg aacagcataa ccaagacatt tgcgatatgt taaattatta cggtctacat
    21181 gctcgaacgc ttgaacggcg caatggctat atttcttatt taaaaggagc agaaaaaatt
    21241 gctgatttct taacattgat tggtgcaacc aactcaatgc tccgatttga agatgttcga
    21301 atcgtacggg atatgagaaa ttcggtcaat cgtctagtca attgtgagac agcaaatctg
    21361 aacaagacga ttgatgcggc ttctaaacaa atcgaaaaca tcgaattgat cgaagcaaag
    21421 gtcggtctac atgcccttcc tgaaaaactt caggaaatcg cagaacttcg gcttcaacat
    21481 ccagaggtca gcttgaaaga attaggggaa atgattccct caggtgcgat atcaaaatcg
    21541 ggaattaatc accgaattcg caaaataaat gaatttgcgg aaaaactgcg tgaagaagca
    21601 gtttaatcca aagaaaaaac tgatacatga agcaggagat gcgaatgtat cagttttttt
    21661 taaaagttat acttgctttt cttccgtcaa actgtaattc catctttact ggagtaacat
    21721 aatttggaaa ggcttctatt gtttttaata caatcgtata aaaagcgggg agacaactga
    21781 atgaagattt tagttttgta cataaatttt tgcttaatat agaagtcctg atatagataa
    21841 gatcaagtat ttatctataa ctggcctgat taattaaaaa actggaagga actgctcgat
    21901 catccttgag aactgctctt cttacttctg gtcaagattc tcgtggttgg tggataagga
    21961 tggttgataa gttcattgga ttagctagtc gaaaacaacc aggtgtaatt aatgtagaag
    22021 ttttaacaag gggatggagt gatttattca gtgataatca taagttcata aatagtaatt
    22081 gagatgtatg gttggaatac gttatacctg aacttgaact accaagattt ggtttagaat
    22141 ttgagaatcg atgtttataa agggagggca aaaatgcgtt tttttacaac ttttactatc
    22201 attatgattg cagtactttt tatttttcta gatatagcaa agcgaaatac agcattttta
    22261 ttatatcggg ttttacttcg agcaggtctc ataacattta tatcgatcgt tggatttttt
    22321 ctattcacag ttattgtatt tatttggaga actccagcac caccattgcc agagattaca
    22381 tatggagaat ttccttttag acttgagtat gaattgaatg aagaattaca tgtgatagaa
    22441 gacacgttaa tagttgaatt tgatggattt ggaatgaacg aaggcatagg gagatatcgt
    22501 agatggacaa gtcgtttagc cagcggagaa gatttagtgt tgctgttaga agttagtgat
    22561 aataagcaaa tattttattt tccaggacct gctaattatt acatgggaga taggttgaat
    22621 ggttataatc atacttttcc tagtgcttct ttcattgaaa gggaaagagg cattactaga
    22681 agggatattt tacatgacaa agaattatta gaacaatttg gtccgcttga tcaaaataca
    22741 ataaatgaag aagaattgtt aaatcaatat aatataaggc tagtgaattg ggaaatatct
    22801 gaaccgattg ttaacaattt tggtgattag aaaacaatta cgataaatag aaacaagcca
    22861 aatcatcagc aagtaaaagc tgatgatttg gcctttctta tacgagtagt aggaattcat
    22921 agcgaatatt attacgatca gtagatattt tgatattaaa ggagtattac gaactagtat
    22981 gtgagaatgt agaggtgatt tgcgaataat gagaaattta tcaaatagaa acaaaatatt
    23041 aatcattatt gttgttatag ctgtgtttca tctgggaacg aatgcggttt taagtagaat
    23101 aatattgggt cctaagccac caagacctgc gattacccga ggggagtttg attttagatt
    23161 agaatatgaa gtagatgggg aaaggattgt gatagaagat acaattgttg cattatttga
    23221 tggattttct gcagatgcag gtagcatggc atggtatcgc acgtggcgtt tgcatttggc
    23281 gagtgacaag agggcacaac aattattgtt acatgagtta gatgatggtc gaaaaatata
    23341 ttatcatttt ggaggagctg gattttatat ggggaagtta gtagcagtgt aactgtaaca
    23401 aggaaagatt tgccaggtct tggcattcga aactccatat tttatatcga gcgtgggaga
    23461 gatacatcta ttgggaacag gaaagggtta actcttgaag agttatataa tgactttgga
    23521 atccgtcttg ttagttggga acacaatcca ccaatagaaa atcgctttga ataatcgaaa
    23581 tctattaatc catttcatca gccagtgaat gttgtaatgg aacaatttgg tttagtgttc
    23641 aaaaccattg aaatactagg ctgtgatcag caaaaatgta tctgaataaa aaagagagca
    23701 actattaaat gagaggagtt aggagttgtt agctttatac gaatcaaagt ggcaaataca
    23761 gatgcgtatg catttgtgtt tgccatcttg cgtttggtag acgagatagg tataagctaa
    23821 agaaacaact tgtttaaaat aaacgaccta gctcaacaaa cgaccatcta atcaactaac
    23881 agcatacata aaagcaacta tttttgaagt agaattgaaa tcgggataac ttaaaaagtg
    23941 attgggatac tagtgtgaat caccgtacat aaatattact cgttaaggga atttgagaaa
    24001 ttttgtatga aaatttaaga attagttagt atgcgcaagt caaaaatatg aataaattat
    24061 ttggtggaga taatttgctc agcttgtcat aagaaatgag ataatctggc agatgcaagc
    24121 ttttggacac catttattcg gaataaggag gctgggacag aagcgtttaa ctccaagaaa
    24181 taagaaggag ttgcttctgc ttccgccgtt tataggtgtt tagagcgtga gacaaaagtg
    24241 ttctttactt ttgtcctacg ctccttattt ttttattttg tttggatcaa ttcttttaat
    24301 tcctctattg aaatagattc atcaaagatt tctttaccca agatgacagt cggaacaaat
    24361 cgaatatgtg cttgctcagc ttcgttgatg acagcttggc tcagctgttc gtctttttgt
    24421 tctgtcagct ttagtttatc tactgcaaaa tccgctactt cttggagaga aagatgtttc
    24481 cactcgtctt gagtatcgaa tatttgtttg atctctttga tcgctttttg accatcgctg
    24541 attgtgagat accggtgcat cacgtttcct cgctgcaagc tttctttttc tttatcgaac
    24601 aatttgatca ctcgctgcaa ttgcccgctt tggactgctt cttctaatag ctcataagat
    24661 tcttcaaacc attgtcggca atacggacaa gataggttga tgaattcgac cattttttta
    24721 ggagcattcg atgaaccata aaggattcct ttttgtgtat ttgttttcgt tgcatcaatc
    24781 actgatatat ccatgttaaa acacctcttt cgcttatagt ttatacatgt ctgacaaaaa
    24841 aaggcaacga tttagactgt ttgtctaaaa attagatgac caattcaaat agacatatgt
    24901 gaaatatttt tataaataaa agaaaaaatg gaatgtttta cgttataatg gaacagataa
    24961 taagagagaa tatgaacaag gatgatgaat catgcgaaaa atgaaaacaa tggatggcaa
    25021 tacggcagcc gcatatattt cttatgcatt taccgagatt gctgcaatat atccaataac
    25081 acccagttcg accatggctg aattagtaga tcaatggtca gcagcaggaa aagaaaatgt
    25141 attcggtcaa ccagtaaaag tagtagaaat gcaatccgaa gcaggagcag ctggtgtggt
    25201 ccatggatca ttgaaaactg gtgcactgac aactacatat accgcttctc aagggctgct
    25261 tttgatgata ccgaatatgt acaagattgc tggggaacta ctgccaagtg ttttccatgt
    25321 tgctagtcgg gcagtgacga caaatgcgtt gaatattttc ggagatcacg gagacgtgat
    25381 ggctgcaaga caaacaggat ttgctatgtt agctgaaagc agtgtacaag aagtgatgga
    25441 tttagcacct gttgcccatc ttgcttctat tgaaggagaa gtaccttttt tgaatttctt
    25501 tgatggattc cgtacgagtc acgagattca aaaaatcgaa gttttggaat atgaagaatt
    25561 agctcctctg atcaatcagg agaaattagc ttcatttcgt aaacgaggga tgaatcccaa
    25621 tcatccgact gttagtggta cgaaccagaa tccagacatc cattttcaac aacgggaaac
    25681 gatcaacccc tattatgata aattacctga aatcgttcaa aaatatatga aagagatcaa
    25741 ccgattacga ggaacttctt atgacttggt gacttattac ggagcagacg atgcggaaga
    25801 agtcattgtt tctatgggat cagttgcaca gacgattgaa caaacgattg attacttgaa
    25861 tgcaaacgga cggaaaacag gttttttgaa tatccatctt tatcgtcctt ttccagtgga
    25921 aacattcgtg caaaagttgc ctcatacagt aaaaacaatt gctgttcttg atcgaagtaa
    25981 agaaccaggg gcaggtggag aacctctctt attagacgtt caaagtgcgt tatatgattc
    26041 tgaacttcgc ccggctgtta ttggtggtcg ctacggattg gggtctaaag atgtgactcc
    26101 cgaccaaatc tcagcagttt ttgatgaatt gaaaaaagac ccaagcatac gcaaaaaacg
    26161 gtttaccata gggatcgtgg atgatgtgac ttatcaatct ttggaaccaa aggaaagtct
    26221 ggatttgaca gaaccgcaaa cattccaggc caagttttgg ggatttggat ctgacggaac
    26281 agttggagca aataaatcag ctatcaagat tattggagat cacaccgata aatatgcgca
    26341 aggctatttt tattatgatt cgaaaaaatc tggcggtctg actgtctctc atcttcgttt
    26401 tggagatacg ccgatacgtt cagcgtatct tgtcgagcat gcggaccttg ttgcctgtca
    26461 cacaccagct tacctgcata gctatgactt ggtcaaagga ttgaagccag gtggcatttt
    26521 tttattgaat actctttgga gcgacgaaca attagaaacg catctgccct taaaattaaa
    26581 acgttacttg gctgaaaata acattagatt ctatacgatc aatgccatga gattggccca
    26641 agaagtcggt ttgggacgcc ggatcaatac ggcaatggaa acggcttttt ttaaattagc
    26701 tgacatcatt ccttttgatg aggtgcttcc gctgctaaaa gaagaagccc tgaaaagcta
    26761 tggacataaa tcaatggcta tcgtagaaaa aaacatacaa gcaatcgatc ggacgaccga
    26821 actgcttcat caagtcgcga tcccagacca ttggagaacg ttggagatgc ctgaaaaaat
    26881 caataaaagg aacactggtt atatacaaga aatacttgaa ccagtgaatg cacaggaagg
    26941 aaatcaactc tctgttggta cacttgtagc aaatggaatg acaactggcg aaatgccttt
    27001 aggaacggct tctatggaaa agcggggcgt cgctttagaa gtacctgagt ggattagtga
    27061 tcgctgtacg atgtgtaatg aatgtgcctt tgtttgtcct catgctgcca tccgtccatt
    27121 tttagcagat gaagaggaaa tggaagaagc gccagaagga tttatcgtga gagaaatgcg
    27181 aggcgcagac ggcgtaaaat accgtatcca agtttcagtg gaagactgta caggttgtgg
    27241 tctgtgtgta gaggcttgtc ccgcaaaaga aaaagcttta gtgatgaagc cttatgaaga
    27301 acagaaagaa caagctgtga attgggcttt tgcaatgaca ttaagacaaa aagcaaatcc
    27361 agtcaaagga aagaatactg tcttgtctac acaattcaat caaccgcttc ttgaattctc
    27421 gggcgcttgc tcaggctgtg gcgaaacacc ttatgtaaaa ttattgacac aaatgttcgg
    27481 tgatcgaatg atgatcgcga atgcgacagg ctgttcttct atttggggcg gtgcttctcc
    27541 tgcttctcct tatacgacca atgaatgtgg tcaagggcct gcatggtcaa attctttatt
    27601 ggaagataat gctgaatttg gttatggtat gcttctagcg gcacagactc gtcgcgaggc
    27661 tttggcaagt tcaatcaaag aagtattgcc aaaagtatca gaagaactac gagaattgat
    27721 ggaagactgg ttggtacaca tgtttgagag cgaaggaacg cagcaaagag cagcaaaact
    27781 gcaagctgcg atcgaaagcg aacgatcaat tgtccctgaa ctggaggata tatacaagag
    27841 aaaggatcta ttcgtaaaaa acagccaatg gatgattggt ggagacggat gggcttacga
    27901 cattggtttt ggcgggatcg atcatgtact tgccagcgga gcagacgtga atattcttgt
    27961 tttggataac gaagtctatt ccaatactgg cggtcaaacc tcaaaagcaa caccagcttc
    28021 ggcaatcgct aaattttcgg ctagtggaaa gtatgcttca aagaaagatc taggaatgat
    28081 ggcgatgaca tatggaaatg tttatgtggc gcaaattgct tcaggtgcca atcaaatgca
    28141 gacgataaag gcttttgaag aagcagaaaa gtatcctgga ccttctttaa tcattgccta
    28201 tacgccttgt atcacacatg gtttgcttgg tggaatgaaa gaatcgctta aagaagcaaa
    28261 agaagctgtt tcttctggct attggtcatt atatcgatat aaccctgcaa tggtcgaaaa
    28321 aaacaagaac ccaatgactt tggactacaa aaaacctgat ttctctgcta tgcctgattt
    28381 catgaggaaa caagttcgct tttcctcgct agagaacgca aatccaaatc ttgcaaaagc
    28441 tttatttgaa aagactatcc aagacgctaa aacacgtttt tacaactatg ctgcactgac
    28501 gggagaatta gaaaaaatca agaaacgact tgaaccagag gaatctatcg atgtacccgt
    28561 taagactaga aaagaaaaaa cgacggacct tgaagcagaa gaaagaagag ctgccagagc
    28621 agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat aacagaaaaa
    28681 atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa taaaacgcat
    28741 acttgcaatt ttcctgaaaa ttatgcgttt ttctcaagaa aaatacgagc agatcagcct
    28801 ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga gaggcttttt
    28861 atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat gttttccggt
    28921 gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt ctggtatctt
    28981 gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct gaaaggtgta
    29041 gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac cttgtcttgg
    29101 ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt tcagccggaa
    29161 atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc aaaaagcgaa
    29221 cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata tatgtccaag
    29281 cgaaaaatcg gtgcgctaat tactatcgaa cggcatactg gattagatga gtatattgag
    29341 acaggaattg cattagatgc agatatcact ggagagctgc tgatcaatat ctttattcct
    29401 aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc agtagcaagc
    29461 gcctatcttc ctctatcaga aagtatgttg attccaaaag aattcgggac tcgacaccgg
    29521 gcagctgtcg gtataagcga agtcagcgac gcgattacca tcgttgtctc tgaagaaact
    29581 ggggatgtca gcattacatt agataatgaa ctgatggctg gcttatccca gcaagaatat
    29641 ttggcaattt tgcgtagaga actgattgct gaagagaaaa caaaaaacaa gaaaaacgtg
    29701 attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc tcaaaagaga
    29761 aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg ctgtacttca
    29821 atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat aatcaaacag
    29881 ttactggggt gccaatcaag atttcttatg attcaaagcg ctactatatc catggatatg
    29941 aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat gcagaggcaa
    30001 acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc gaaggaacac
    30061 atgaggttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag atcgaaccat
    30121 cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg gaaacgttga
    30181 ttccttcaac gttcacacca tctggatatg aatttgatga tacttccgtt agtcctaaaa
    30241 aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta gtagctgatg
    30301 tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata caggcgctga
    30361 atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca gtgagagctg
    30421 attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga acaccagctg
    30481 ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta agcggctcta
    30541 gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc tcaggtatca
    30601 atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt atcaagcccg
    30661 aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc tctgcttcga
    30721 cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct gctgattcag
    30781 gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca agcagctcga
    30841 ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc agaacaaatt
    30901 cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt ttggaacaga
    30961 cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta agctgggacg
    31021 gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac gcgtgttagt
    31081 agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt caggactttt
    31141 atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg ttgcttatct
    31201 gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata atccagcaca
    31261 agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg accaagagct
    31321 agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat cagctgaagg
    31381 attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat tccttgtaca
    31441 aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa acggagcaac
    31501 ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata ccatgggcac
    31561 aaatccagac gggttaaata tcaatgatgg cgtaggctct acccatccag aacgtttagc
    31621 tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg atggcgatcg
    31681 tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca tgtttatctg
    31741 tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta cgactgtcat
    31801 gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg tcgttacaca
    31861 agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact ttggcggcga
    31921 gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga tgctttcagg
    31981 aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc ttgcagcaga
    32041 ggtaacaatc tatccgcaaa aattagtgaa tatccgagta agtgataaaa acggtgcgat
    32101 ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg gatcagaagg
    32161 aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg cagaagcgcc
    32221 tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca aagaagaaat
    32281 cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag tgtcagatgt
    32341 ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta aattacgtgg
    32401 tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt gagtcggtac
    32461 gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct ggtcactttc
    32521 cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt cgacttcttt
    32581 caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccaa tcactggtgt
    32641 tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa aaccttctgc
    32701 ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt tgtagtcagc
    32761 gccacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg ccaactcttc
    32821 taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac tcaattcatg
    32881 agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag ctttggctaa
    32941 taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg cgatttcggg
    33001 tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac cagcaacatt
    33061 tgtaatcgta agtgaagggt agccgagacg gttgattttt accagaactt ggcggctatc
    33121 tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa gaagaggtgt
    33181 gttatagcca aattcactgg ctaaatgaac ctcaacaggg atctgtgtca atttttccag
    33241 agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga tatagattcg
    33301 atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat tgtcttctgt
    33361 tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt ctttcagcat
    33421 ataaaaagga taggtacctt tctcgatatc tgaagaatct acttgtgctt cgaatgaaga
    33481 acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg tgacggtaac
    33541 catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag ccattgcatc
    33601 gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt tatttttagc
    33661 gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag aacctttgat
    33721 gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga aagtttcaat
    33781 tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg ctaaataagc
    33841 aattttaagt tcttcaaagt tttcgattac gccattgtga acgagcacga agcgtccact
    33901 ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg cccagcgtgt
    33961 atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca aattttgaat
    34021 tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga taccagctga
    34081 atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg ttacattttt
    34141 taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta taatgaattc
    34201 gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga tattttttgt
    34261 ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat aaaccctctc
    34321 ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat cctcctcttt
    34381 tgttttttat ctggaaacat ttaaaatcat aaccttttgc gaatgttctt gtcaacaaaa
    34441 aaagaataaa aaataaattg gtatatatca aatgctttgt caaatgctct ttttatgtca
    34501 aaaaataatg ggtttttatc tatcatcttt ttcaggagtt cggtacaata gaaagtaatc
    34561 ggatagagga ggagtaaaaa tggattcaaa aattgagatc atgacactgg gaatgttaaa
    34621 aaaacaattg tccaaattcg aagcatcagc tggtgtatca gatgatacga aaatattttt
    34681 agatactggc tgggatagca tccaagaaat cgcacctgat gccttggaag ttgctgaagc
    34741 tcgtgagttt acggtagaag atgaattgac aaaagagtcg ttttccgggt atgcgcggga
    34801 agaaaaagca gagcggttcg acacttctga gcagtcagaa actgtgatcg tcataaaaaa
    34861 cttgtattag aaaggagaaa gaatgaataa gagacgttgg gcagcagttc tgattgctgc
    34921 aggattattt gtggtttcgt tagtttcagc tagtttgact aagcctcagg aaaacgatca
    34981 aattgaggga ttgaacagtt ggttatatgg ggatgaagaa ctagcttcaa atgtgttgga
    35041 agagggagac agtacgcaaa aaatcgtaaa attatcagta gatggaacga ttgctgcggg
    35101 aggggcttca ggtttatttg cttctgaagg ttacaatcat aaaaatttta tggaacaact
    35161 gaaggcgatt gaagaagatc caacagtcaa aggtatcttt ttagaagtaa attctcctgg
    35221 tggcggtgtc tacgaaagtg cggaaatcgc taagaaattg gatacgattc gtaaagaaca
    35281 tgatatccca atgtatgtca gcatgaaaaa catggcagct agcggtggct actatatttc
    35341 tgctcaagca gataaaatct ttgcaacaga agaaacagtg accggctcaa ttggtgtgat
    35401 catgtcagga ttgaattatt caggacttct agaaaaactg ggtgtggaag atacaacagt
    35461 taaaagcgga gcgttgaaag atatgggttc aggaacgcga ccagagacaa aagaagaaga
    35521 agcggtgctt caagcatata tcgacagcgc atatagtcgt tttgtagcaa tcgtcagcga
    35581 gggacggaat aaatctgaag aagaagtcaa aaaaattgct gatggaagga tttatgatgg
    35641 tgtccaagca aaagaagtag gtttagtcga tgaattggga tttcctgaag atgctttaaa
    35701 agctatgcga aaggaacaac agcttgaaga tgcggaacta gtagaatatt cttccacgcc
    35761 atctgggttt gctagtacat ggttaggaag taaactagca gaattccagg gattgaagcc
    35821 ttctgagact agtcagatct tatccgttct tgagaatcta gggacagcgg aatcaccaaa
    35881 agcaatgtat tattacggag gtgagtaagc atgtcagagc tttcaaatga aaataaggaa
    35941 caagaaaaag ataagcaaca gcagcctgct gtaggacctg actccaactt ttcccaaaag
    36001 gtactgagct cttttgaaag agagcctttg acgcccgaag aaatcaaaaa gaaacaacgt
    36061 aaatggaaaa agtatacgga cacaagaaat gaaccgccaa aagcaaatga ttttcctaag
    36121 tatttctatg caggtttttt tatccgaatg tttgctttct tggtcgatct tttgtgtatc
    36181 ggagctatta cgcgtttaac acttggtatt gccaaaaatc taggttggct taatttttca
    36241 gattcttatc tgagtatcta cggattcaca gccttactta tttatctagc gtattttatt
    36301 ttattgacga aattcaatca tggtcaaacg attgggaaaa tgatctttgg tattcgagtc
    36361 atcagctttg atgaagaaga acttagctgg tcaaccgttt tagttagaga aggtgcttgc
    36421 cggttcattc taaaatttcc tttgctgatg ttagggtatc ttccagcaat ctttaatcag
    36481 aaaaagcaac atctaggcga ttttttcact aatacaagtg ttgttacgat caatctgatc
    36541 aaagcattca accaggaagt caacagctga tgagcaataa aaagcagtaa cagttgaaag
    36601 caccttctaa aagtcagatc taaaatctag cttttagagg gtgcttcagt tatttttaat
    36661 tgtcatgctt tttttgtttt gctactattg aacatgagct gaacatgagc aagaggagga
    36721 cactgcgtga agccgattga ttttccagaa aaatatgaga atttaatgcg agtagcacaa
    36781 caagcactag caaaccagca atatcagcag gcaaaagaat tatttcaacg agcttacgag
    36841 ttgaaagaaa gctttgaggc taatagcctg cttgtttttt gtctttacga gttggacgag
    36901 aaaaaagaag cattgaagca agcactcttg catgaaaaac agtatcttca aaatgaggag
    36961 tttgctgaat tttattttga cttgttaatc agtacacaag attttttata tgctagaaaa
    37021 ttgatagcaa gtacagattt ttatgaagct ttcgagcagc gggtcattga aaaaatccaa
    37081 tttgcagaag aattatccgg acaaatggag aggcagaaga tcaaggcact ccacaataaa
    37141 tcggaggaac ttccttcgtt ggaacctgca cgtcagcttg cgtctattga tgagatcgaa
    37201 cagcttcctt atcatgagtt tatccaaaca gcgagtaaac ttattgtatc gccagaggtc
    37261 catattttag caagggcaaa attactggag acacttaggc aattgaacga atgccatcct
    37321 gttttctatt tgacgattga agaaaagtta gtcaaagtca taccaaaaga tctgccaaag
    37381 ccgcagcagc aggcaagtta ccagcaatta tgtgtatttt cagatcgtta cggtaatgaa
    37441 gatgcgctgc ttagttccgt gttaaatgaa gaattcacgt tgcaatctgc aattgtttat
    37501 cctgtctatg atacatacat cgaagacccg aagcgctggt ttcaactgac tgtagaggcg
    37561 tgtacaggaa aaacgatgta tgaaggtaca gaagatgaaa aacaagattt tttaaaaaaa
    37621 agagaaaaaa ttttgcagca aatgatcttc ttccattaga ttttatcagt gaaaaagata
    37681 aaaatgttat gaaaaacaag gattctatcc attcaatatg tttacaattt gaaagggtta
    37741 gtgtactatt taaaggtatg catgaaacgt ttttgatgta taaaatgaat ttctaaactc
    37801 ggagggaatt aaatgactgc aacatttgaa aaaaaaggca cgaatgatgg tgttttaaaa
    37861 tttacgattg cgcaagacga aatccaaaaa ggattaacaa ctgcgtttaa taaagtgaaa
    37921 aaaagcttga acgtacctgg attccgtaaa gggaaagttt ctcgtcaagt attcaaccgt
    37981 atgtacggtg aagaagcttt atatgaagat gcattgaatg ctgtattacc agaagcttat
    38041 gaagctgctg tcaaagaagc tggcatcgat cctgtttctc aaccaaaaat cgatgttgaa
    38101 agcatggaaa aaggtcaaga ctgggtaatc actgctgaag taacagtgaa accagaagta
    38161 aaattaggac aatataaaga tctagttgtt gaaaaacaag accgtgaagt aacagatgct
    38221 gacgtggatg cacgtatcaa acgtgaacaa gaagcacaag ctgaattagt tatcaaagaa
    38281 gatgctgcag ctgaaaatgg cgatacagta gtaatcgact tcgaaggctt tgtagatggc
    38341 gaagcgtttg atggcggaaa aggcgaaaat tactcacttg aattaggttc aggctcattc
    38401 attccaggtt ttgaagatca attagttggt cataaatcag gtgaaagtct agatgtaaat
    38461 gtaactttcc cagaagatta tcaagcagaa gatcttgctg gaaaagaagc agtcttcaaa
    38521 gtgacaatcc atgaagtaaa agcaaaagaa gtgcctgaat tagatgacga attcgcaaaa
    38581 gatgttgatg acgaagtatc aacattagac gaattgaaag aaaaatatcg caaagaatta
    38641 gtagaaacaa aagaaaaagc agcggacgat gcaaaagatg aagcagcgat ccgtatggct
    38701 gttgaaaacg cagaaatcgt tgaattgcca cacgtgatgg ttcatgatga agtacatcgt
    38761 tcaatggacg aattcttgaa caacatgcaa cgtcaaggta tctctcctga aatgtactat
    38821 caattaactg gttctacaga agaagacttg cataaacaat tcgaaggtga agcagaaatg
    38881 cgcacaagaa caaatcttgt gatcgaagca attgcagctg ctgaaaatct tcaagcaaca
    38941 gaagaagaaa tcaatgcaga aatccaagaa ttatcagaaa catacaacat gccaatcgaa
    39001 caaatcaaac gcgtgttgac agaagatatg ctggaacatg atgtgacaat gaaaaaagca
    39061 atcgaattga tcacaggtac agcgacagaa gcgaaagaat agaaatcaag taataaatca
    39121 tttctgaaga agctacttct agaaaattgt ttattcgaaa taagggactg tgacacgatt
    39181 ttgtcacagt tccttatttt ttgttaagag taaaaaatgc aaaggtatag gtcagttgat
    39241 cctttgttaa acactttttc tttttactaa actatgttat gatagtaaaa gactgtaaag
    39301 tctttgatag tttcttgcat actttttgca agttaagggg tgaagaccat gtatgacaat
    39361 ccaagtagca atgagaccgt acgctgttct ttttgcggga aaacccaaga agaagtgaaa
    39421 aagatcgtag ctggtccagg ggtctacatc tgtaatgaat gtatcgacct atgtaaagaa
    39481 atcattgatg aagaatttta tgaagaagct gtacgcgaat tcactgaagt ccctaaacca
    39541 ctagaaattc ttgaagtatt gaataactac gtgattggac aagatcgcgc aaaacgctcg
    39601 ttagctgttg ccgtatacaa tcactataaa cgtgtcaatc agcaagcaca agaaaacaca
    39661 gatgatgttg aactgcaaaa aagcaatatc tgcttgatcg gacctacagg gtctggtaaa
    39721 acatttttag cacaaacatt agcaaaaaca ttgaatgtac cttttgcaat cgcagatgcg
    39781 acaagtttga cagaagctgg ttatgtgggg gaagatgtag aaaacatttt gcttaaattg
    39841 cttcaatctg ctgattacaa tgtggaacgt gcagaaaaag ggatcatcta tattgatgag
    39901 atcgataaaa ttgcgcgtaa aagcgaaaat gtatctatta ctcgtgacgt ttccggcgaa
    39961 ggtgtccaac aagccttgct gaaaattcta gaaggaactg ttgcaagcgt gccacctcag
    40021 ggaggacgta aacatccaca tcaagaattt attcagattg atacgacgaa catattgttc
    40081 atcgtaggtg gtgcatttga tggcatcgaa acgattgtga agaatcgtat gggcgaaaag
    40141 acgattggat ttggtacaca aaaccaaaaa ctttctgaag acgaaagtgt catgcagcat
    40201 atcattcctg aagatttact aaaattcgga ttgatccctg agtttatcgg acgattacct
    40261 gttatggcag ctttagagaa attgacaaca gatgatctgg tacggatctt gactgagccg
    40321 aaaaatgcgc tagtcaaaca atatcaaaaa cttctttctc tagataatac cgaacttgaa
    40381 ttcgaacctg aagcgttgag agccattgcg aaaaaagcaa tcgaacgcaa taccggtgct
    40441 cgaggacttc gttccatcat tgaagaaatc atgatggatg tgatgtttga tatcccttca
    40501 aatgaaaaca tcgaaaaagt gattatcaca aaagaagcag ctgaattgtc tggtgagcca
    40561 acagtaatct ataaagattc agagaaaaaa gcgggttgat gaaaagaggc tgccgtaaaa
    40621 gtcattttgg cttttgcgac agccgcttgc tttattcatc accttgttaa ggagagagac
    40681 catgaaagtc catcaagctg aaattatcat aagtgcagtg gcaccaaaac aatatccaga
    40741 tacacaattg ccagaaatag ctctggcagg acgatcaaat gtagggaaat cttcttttat
    40801 caacacattg attgatcgaa agaatttagc ccgtacttcc ggtaagccag gaaagaccca
    40861 gacattgaat ttttatttga tcgagaacgc attacatttt gtcgatgttc cgggatatgg
    40921 gtatgcaaaa gtatccaaaa ctgagcgagc aaaatgggga aagatgatcg agacatattt
    40981 aacgactaga gaccagctac gtgcggtcgt ttctttagta gatttgcgtc ataagccttc
    41041 tcaagaagat atccagatgt atcaattttt gaagtattat gatatccctg taattgttgt
    41101 tgccactaag gcagataaga tccctcgtgg taaatggaac aagcatgaga gcgaaatcaa
    41161 aaaagcgttg gattttgatc gcaatgacac gtttattctc ttttcttccg ttacaaaaca
    41221 gggaaaagaa gaagcgtggg cagcaatcga acaagcaatt gaatcataaa aagaataaaa
    41281 aagacttacc agtcaaactc tttcataaaa gtttggctgg taagtctttt tttgagctta
    41341 acttactttt gtttcttgtc ttttttcttt tcattttgtg gctgtggttt tagaaatcga
    41401 tcgatctctt cttttggtgc aactgttttt tccggatcga cagcaaatga ttcgaaaagt
    41461 ttatctaaat tatctgtacg ttcaaatttc aaccccattg taaacacctc tttgtttctt
    41521 tttacttagt ctatcatgta atcgtccaaa ttggtttcta agacataaag ttttagtttt
    41581 tctttatcgt tggttataat taaattataa aaagatttta aagaggtgag aaaatgattg
    41641 gcgaagataa attattgatg actatccagc tcacagactt aaatgaagaa gaaaaaaaac
    41701 aggaaataag cgattgggct attttgacac gtctgttgat ccagccaaca tttatccgct
    41761 ctttcacaca acaagcggat ccgtatgatc ttcgaaagct tcaagaaaaa ataatgctag
    41821 catcggtaag agacgaaagt tggttagttg taggaaatga cgagaacgaa tgtagttttc
    41881 gcttggaaga taatcaatta ttgataaaaa atgtattgtc tatctctgtt ttcaaagaaa
    41941 agcaattttt gattcgagac ttcattcaga aaaaaatgat tgctcatggg gtgttcgcct
    42001 atatgcgagc ttatagcgag tttatctatc acaatacaaa acagatctct caacggctat
    42061 tgtttgagaa aaaggatgaa atcgaacatt tacctaaaat gaaacaacaa aacggggaag
    42121 tagtagtcga ttgcaaccaa tttccaggat acgatctttt ttaccaaggt ttatgtttca
    42181 cttcgtgttg ggaaatgtat tatagtcgct attatcatca aatcatccct aaaccgattt
    42241 ttctcgacat ccaacaagta gaaaaagtga aggaattaga aaacgaagtc atatgtatcc
    42301 agctttatcg agaccccttt aactggcaaa agccaaacaa tcaaatgttt caatcctatt
    42361 ttagggacca gcttgggttt gatcatcttg cttgggacaa tggtgtcggt ttattgaaac
    42421 cgccattcgt tgaatacatt tacactgacc atacgatcca atctgtccag tatcagaatg
    42481 cacaaatgca acctgttcct aaaaagaatg cttcattttt tgtaacaaaa agttatgata
    42541 ttcaacaagg agattataaa gaaaggcgtg ttcgcggaac actaaatgct caggcgtatt
    42601 ttccttgggt tgacgagaat cgctcgcgca tgatgtgcta taaagttatc gatccgactg
    42661 ttgctttgga taatggaatc gaagcttatt gttattatat tagggaatat ctagaagtag
    42721 aggtaacaga tgaaaaatat caagagtatc ttttatcctt aagaatctat gttccttcag
    42781 aatccctgtc agaattaccg ttaaaggaaa tcaaacatag actctctgat gttaccttca
    42841 aacgaataaa gaaaaagcgg cgaagtatcc aattcgatgt caagaaaggt gagcagcatc
    42901 tgcgtgtgca atttttagat taccgagaat tagaacaatt gattacttta caaaaaatat
    42961 gaggtgaaga aaatggataa acaactctat tatcgaataa tggttccggc atcattatta
    43021 tccgtctgtc tcttttttgt tcctaatttt cctattcgat cagtcatcgg ttttttggtc
    43081 atgtttggcg ggatgggact ttattattat cttcttcata aggaaagaaa acctgcttct
    43141 gtttatatga aaaaaacaag aagcgaaaat aaaaaacgat aaaacggct
//
LOCUS       DAZUMV010000010        41721 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000010 DAZUMV010000000
VERSION     DAZUMV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.010"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACG8FK_000499"
     CDS             complement(159..1469)
                     /locus_tag="ACG8FK_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS5096136.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="ACG8FK_000500"
     CDS             complement(1481..2899)
                     /locus_tag="ACG8FK_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5096137.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="ACG8FK_000501"
     CDS             complement(3139..4707)
                     /locus_tag="ACG8FK_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS5096138.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="ACG8FK_000502"
     CDS             complement(4711..5121)
                     /locus_tag="ACG8FK_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS5096139.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="ACG8FK_000503"
     CDS             complement(5118..6041)
                     /locus_tag="ACG8FK_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS5096140.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="ACG8FK_000504"
     CDS             complement(6038..6916)
                     /locus_tag="ACG8FK_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS5096141.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="ACG8FK_000505"
     CDS             complement(6903..8240)
                     /locus_tag="ACG8FK_000505"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS5096142.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="ACG8FK_000506"
     CDS             complement(8243..9346)
                     /locus_tag="ACG8FK_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5096143.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="ACG8FK_000507"
     CDS             complement(9420..10928)
                     /locus_tag="ACG8FK_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5096144.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="ACG8FK_000508"
     CDS             complement(10894..11586)
                     /locus_tag="ACG8FK_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5096145.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="ACG8FK_000509"
     CDS             complement(11608..12582)
                     /locus_tag="ACG8FK_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096146.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="ACG8FK_000510"
     CDS             12882..13754
                     /locus_tag="ACG8FK_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5096147.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="ACG8FK_000511"
     CDS             complement(14212..16410)
                     /locus_tag="ACG8FK_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5096148.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="ACG8FK_000512"
     CDS             16642..17634
                     /locus_tag="ACG8FK_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5096149.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="ACG8FK_000513"
     CDS             17694..19115
                     /locus_tag="ACG8FK_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS5096150.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="ACG8FK_000514"
     CDS             19108..20607
                     /locus_tag="ACG8FK_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS5096151.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="ACG8FK_000515"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="ACG8FK_000515"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5096152.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="ACG8FK_000516"
     CDS             22199..22882
                     /locus_tag="ACG8FK_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS5096153.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="ACG8FK_000517"
     CDS             22884..23720
                     /locus_tag="ACG8FK_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5096154.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="ACG8FK_000518"
     CDS             23731..25485
                     /locus_tag="ACG8FK_000518"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096155.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="ACG8FK_000519"
     CDS             complement(25761..26102)
                     /locus_tag="ACG8FK_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5096156.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="ACG8FK_000520"
     CDS             complement(26170..28341)
                     /locus_tag="ACG8FK_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5096157.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="ACG8FK_000521"
     CDS             28552..29409
                     /locus_tag="ACG8FK_000521"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5096158.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="ACG8FK_000522"
     CDS             complement(29480..30337)
                     /locus_tag="ACG8FK_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5096159.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="ACG8FK_000523"
     CDS             complement(30322..33024)
                     /locus_tag="ACG8FK_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5096160.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="ACG8FK_000524"
     CDS             complement(33037..34326)
                     /locus_tag="ACG8FK_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS5096161.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="ACG8FK_000525"
     CDS             34476..35522
                     /locus_tag="ACG8FK_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5096162.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="ACG8FK_000526"
     CDS             35524..37677
                     /locus_tag="ACG8FK_000526"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS5096163.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38150..39601)
                     /locus_tag="ACG8FK_000527"
     CDS             complement(38150..39601)
                     /locus_tag="ACG8FK_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5096164.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39598..41322)
                     /locus_tag="ACG8FK_000528"
     CDS             complement(39598..41322)
                     /locus_tag="ACG8FK_000528"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5096165.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41315..>41721)
                     /locus_tag="ACG8FK_000529"
     CDS             complement(41315..>41721)
                     /locus_tag="ACG8FK_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5096166.1"
                     /translation="LRGNLLFSLFLSGTALLSYNLYLSVQIKGLIFLAIDFILLFALL
                     CLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSGIILGVTWQFKGL
                     ILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
BASE COUNT        12281 a         8545 c         7365 g        13530 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt
    37861 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta
    37921 gtggagaggt cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtgaaaatg
    37981 gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt tgaactccgt
    38041 ttatcgagag gtcatgaaaa agctgccttg cctcaagtaa taagaacagc ccaaaattag
    38101 atttctagac aattttttat aagttatttc attacattgg ctttttcttt tatgattctc
    38161 cactgttttc tcggtactct ttaggggatc tgccgacgat tttcttgaat aatttagaga
    38221 aatacgtggt gttgttgtag ccaatctcac ttgctatctc attgatgttt tgattactgt
    38281 gcaaaagcag ttgtttcgct ttttccatac gtaaatggtt caaataagct gaaaaacttt
    38341 ttttcgtttc ttttttgaac agctgtccta gatacatgac atttaaatgc aggttttctg
    38401 acacttcttt gagtgtaagt ggttcttgat aatgctggtg caggattacg attacttttt
    38461 ccacattgtc actgtacttt ttctgtctct gatgcttttt gatgaatgac agaagtagct
    38521 gctgaagttc ttgcaccgtt tttgcctgat ggattttttc gattccttgt aaatattcct
    38581 catcttccag atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa ctggaatatc
    38641 gtatatcttc cggcatcatg gctgctaact gaaactgttc aaaaaaatca gtgatcattt
    38701 tttgagcgac ttccaattgt ttattttgca gcacgcgtct gaaacttgaa aaatcgatgg
    38761 tttgatcttt tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta tctccataaa
    38821 attgatgcaa ctgtagattg tctttcgctt gttggaatgt ttcagggatt ttgtccaaag
    38881 aatctgtctc ttccccgata ctgatcagcc attcctgatc aggaaagttt tcttgaacaa
    38941 aacaacagaa gccatcaact tcatctgcct ccaacaacaa cgtgatcaat acacggtctc
    39001 cgtaatttcg ttgatagtaa aagatttgtt gccgttcttg caaccatcta ttgataagag
    39061 tatcatgagt tcttgccagt tgaatcaata ctatcctatg ctgcttctca cttatttgtc
    39121 ctattatttc ttcttcactt atttcatcca attcttcatt caaccattga ctgaataaca
    39181 gttcttgata gatttcttgt tgattttttt gttcattttg ctgatctaac cgtgttttga
    39241 ttttcttgag gctttcaacc aattcgaact tattcacggg tttcatcaaa taattcacgg
    39301 cgcctaattg catacctcct ttcaaataat cgaattcttg gtatcctgat aaaatcataa
    39361 attcaaaatt atgacgttct ttttgagccg cttcaataaa ttccagtcca tttatttctg
    39421 gcattgtcac atccgttact actaaatcaa tctgtcgctg ttcaagtaca gctagtcctt
    39481 ctttggcatt ctctgctgtt gctactattt gaaagcctaa actttgccaa tcaattatct
    39541 tttttaaccc attcaaaatc atgtattcat catctaccaa taacacttga tacacgctca
    39601 tccctccttg aatgtaatag aaacggtcag tccacctgtc tcattattct caagcgacat
    39661 ataataactg ctgccgaagc tggcacgtaa acgttcattc acgttttgca gaccgataga
    39721 accgtgaagt tccactttag gatgagacag tttttcttca atctgtttta gccgtttttc
    39781 agtcatccct tttccattat ctttgatgat gatccttacc cgtttgcctt cctgcaagac
    39841 tttgacactt agcgcattat cgaatcgagt aaaatcaata ccgtgtttaa aataattttc
    39901 aactaatggc tggatcacaa atttaggtac ttcgatcttt tccaaatcag gatcgatcat
    39961 aaaatgatag gctactcgat taggataacg catttgatac aaatagacat atttttcgca
    40021 aaagtccagt tcttctttta aagtgatcgt tttctcttga ttcgtattgt ttcgaagcaa
    40081 agcggaaaac gcatacacaa catctgctaa ttcttcactt ccttcgctga ttgcatacat
    40141 tcggatatat tctaatgtat tatataaaaa gtgtggattg atctgtgctt gaagcgcccg
    40201 catatgtgcg tcttgctgtt tgatttctaa tttgtagatg tcttctacaa actgattgat
    40261 gctatccaac atttcgttga taccgattga aagttccttc agttcatatt ccgtgtttgt
    40321 tatgtcgatc cgcgtctcta aattccctgc tgctgtttga tccatggcct gcatgacgat
    40381 agatacttgc tgtgaatatc gtttgaatgt tttataaaga aagataagca gtatattgtc
    40441 taaaataatg ataccaatga tcagcggggc aagatttttt cccgtttcga tttttacact
    40501 ttttttatct agtacagcta atatacgata atcatcagac agttcttggt gttgaacaaa
    40561 attgtttttt tccagatcgt caatcggcaa ttgtgaattt ttcttcagac tctctgtgat
    40621 gatcatttct tgtctttcaa acgtttcttt agctatttta ttatcatgga atgtgtatag
    40681 tcgatttgtc gttccagaaa tcatgtataa agagagtcca ttgaatgacg ttagattttc
    40741 taaaatcata tctagttctt tcttttgaaa accaataaaa atacttcctg actcagctcc
    40801 tccttgtgtg ataggcaact tgatataaaa ggcgtctagt aattttggtg tccccttttt
    40861 tatttcccca cctttgtttt ctctagaaga ttcataatac tccggtagtt gattcaacac
    40921 gatatatatc gcacttagtt cttcataatt tgcgtagaga tctttgattt gatttgggaa
    40981 agaaactaat tcatcggtac tgttttgctg ttcgtataca tacatcagat actgatctat
    41041 gggttttgtt aagtaggtcg tcacattttc gatcttttcg gaattcccta caagctggtt
    41101 tttgatgatc ctactcaagg ttgttttatc attcagcatt ctgcttgtgc tttgaacggc
    41161 atcacttgtc atgttttctg cctgtttttg attttgttcc caagtatgga tactaaaaat
    41221 aacggtagca gtcatcgtta caccgatcat gataaccgcg tatttccgca acagacgatt
    41281 tagtaaggtt ccttttgaaa atagaatccg aaacttattc ataactttcc aactgtttat
    41341 ctaattcttc acgccaatgt gttgtcatcg taccattcca aaccgtcaaa agaccaatga
    41401 caccaaacag gatcaatcct ttgaattgcc aagtcacgcc taaaattata ccgctaccaa
    41461 taattatttt tagaaaagac gaaaagctgg aaaaaaccga aataaaactt agctttagca
    41521 gattaggaac agagatcgta tattcactat ctaaaatcat actgtattga taagtagcaa
    41581 ataggcagag caaggcaaaa agtaaaataa aatcaatagc taaaaaaatg agccctttga
    41641 tttgaacaga taaatataaa ttgtaactca ataaagcagt accagataaa aataaactaa
    41701 atagcagatt gcctcttaaa a
//
LOCUS       DAZUMV010000011        40092 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000011 DAZUMV010000000
VERSION     DAZUMV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.011"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            587..1813
                     /locus_tag="ACG8FK_000530"
     CDS             587..1813
                     /locus_tag="ACG8FK_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5096167.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(1902..2294)
                     /gene="rpsI"
                     /locus_tag="ACG8FK_000531"
     CDS             complement(1902..2294)
                     /gene="rpsI"
                     /locus_tag="ACG8FK_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5096168.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(2310..2738)
                     /gene="rplM"
                     /locus_tag="ACG8FK_000532"
     CDS             complement(2310..2738)
                     /gene="rplM"
                     /locus_tag="ACG8FK_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS5096169.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(3272..3739)
                     /locus_tag="ACG8FK_000533"
     CDS             complement(3272..3739)
                     /locus_tag="ACG8FK_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS5096170.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3859..5397)
                     /locus_tag="ACG8FK_000534"
     CDS             complement(3859..5397)
                     /locus_tag="ACG8FK_000534"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS5096171.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5477..6163
                     /locus_tag="ACG8FK_000535"
     CDS             5477..6163
                     /locus_tag="ACG8FK_000535"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS5096172.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(6364..6891)
                     /gene="msrA"
                     /locus_tag="ACG8FK_000536"
     CDS             complement(6364..6891)
                     /gene="msrA"
                     /locus_tag="ACG8FK_000536"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5096173.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6975..7652)
                     /gene="pgmB"
                     /locus_tag="ACG8FK_000537"
     CDS             complement(6975..7652)
                     /gene="pgmB"
                     /locus_tag="ACG8FK_000537"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5096174.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7649..8779)
                     /locus_tag="ACG8FK_000538"
     CDS             complement(7649..8779)
                     /locus_tag="ACG8FK_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5096175.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8779..10962)
                     /locus_tag="ACG8FK_000539"
     CDS             complement(8779..10962)
                     /locus_tag="ACG8FK_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS5096176.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10959..11984)
                     /locus_tag="ACG8FK_000540"
     CDS             complement(10959..11984)
                     /locus_tag="ACG8FK_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFS5096177.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANAIRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(12029..12856)
                     /locus_tag="ACG8FK_000541"
     CDS             complement(12029..12856)
                     /locus_tag="ACG8FK_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5096178.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12853..13920)
                     /locus_tag="ACG8FK_000542"
     CDS             complement(12853..13920)
                     /locus_tag="ACG8FK_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS5096179.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13946..14782)
                     /locus_tag="ACG8FK_000543"
     CDS             complement(13946..14782)
                     /locus_tag="ACG8FK_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5096180.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14779..15648)
                     /locus_tag="ACG8FK_000544"
     CDS             complement(14779..15648)
                     /locus_tag="ACG8FK_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5096181.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15718..17001)
                     /locus_tag="ACG8FK_000545"
     CDS             complement(15718..17001)
                     /locus_tag="ACG8FK_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5096182.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(17015..18700)
                     /locus_tag="ACG8FK_000546"
     CDS             complement(17015..18700)
                     /locus_tag="ACG8FK_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS5096183.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18975..20000
                     /locus_tag="ACG8FK_000547"
     CDS             18975..20000
                     /locus_tag="ACG8FK_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5096184.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20595..24248)
                     /gene="rpoC"
                     /locus_tag="ACG8FK_000548"
     CDS             complement(20595..24248)
                     /gene="rpoC"
                     /locus_tag="ACG8FK_000548"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS5096185.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(24327..27944)
                     /gene="rpoB"
                     /locus_tag="ACG8FK_000549"
     CDS             complement(24327..27944)
                     /gene="rpoB"
                     /locus_tag="ACG8FK_000549"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5096186.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSLLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28672..29640
                     /locus_tag="ACG8FK_000550"
     CDS             28672..29640
                     /locus_tag="ACG8FK_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5096187.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29658..30563
                     /locus_tag="ACG8FK_000551"
     CDS             29658..30563
                     /locus_tag="ACG8FK_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096188.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30560..31366
                     /locus_tag="ACG8FK_000552"
     CDS             30560..31366
                     /locus_tag="ACG8FK_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096189.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31523..32491
                     /locus_tag="ACG8FK_000553"
     CDS             31523..32491
                     /locus_tag="ACG8FK_000553"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS5096190.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32808..33332)
                     /locus_tag="ACG8FK_000554"
     CDS             complement(32808..33332)
                     /locus_tag="ACG8FK_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS5096191.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33619..35430
                     /gene="scm"
                     /locus_tag="ACG8FK_000555"
     CDS             33619..35430
                     /gene="scm"
                     /locus_tag="ACG8FK_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS5096192.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(35585..36571)
                     /locus_tag="ACG8FK_000556"
     CDS             complement(35585..36571)
                     /locus_tag="ACG8FK_000556"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5096193.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(36594..37160)
                     /locus_tag="ACG8FK_000557"
     CDS             complement(36594..37160)
                     /locus_tag="ACG8FK_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS5096194.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(37174..37590)
                     /locus_tag="ACG8FK_000558"
     CDS             complement(37174..37590)
                     /locus_tag="ACG8FK_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS5096195.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(38047..39981)
                     /locus_tag="ACG8FK_000559"
     CDS             complement(38047..39981)
                     /locus_tag="ACG8FK_000559"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5096196.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
BASE COUNT        11513 a         8566 c         7482 g        12531 t
ORIGIN      
        1 agtaaactcc atttaacttt tttcgtagtg tgtcttttta tacatgttta tcgttattaa
       61 tagtgaatag gaaaaaacct atttttgatc attgacaatt aaatacaatt tttgaataac
      121 gatccctgct attcgagcta gaaactcaag tacgccatga cctgattagc gacaaatact
      181 tagagatttc tttttgttct gggaaaacaa aatgccatga caatagcagc aaataatgat
      241 acttccgtaa ggctcgccgt aaactgggga gaggtgaaat tcctatgtgc ttacccaaag
      301 caattcaaaa attctaatct tttttaggag atgattttat aaaattgaga gtctacttga
      361 ataaaaaaca aacctgtgag tttctaggaa ttgataataa tactttagat aagtggattc
      421 gtatatataa ttttccagta atcagaattg acggtatagt tcgctttgac atagaggata
      481 tcaaagattt tatgaataca tttaaaacaa tttgaaaatt tcttctcgcc gcatatacta
      541 agaatagcga aatatactgc cttacttttt aagggagtga ggaattttgc ctacaaaact
      601 ttcaaatggg aaatataaaa ctaatctgag gtatccaaaa aaattcagag aaatcactgg
      661 aattacatca gaaaagtatc aaaaagtatt tccaactagg caattggcta ttaaagccga
      721 aaatgcaatg aaaaggaaaa ttgaaacagt tttaagagag gagaatgcaa attcactaga
      781 attaaaagga aaaataaaat ttaaggaatt ctatgaatca aaatggcttc ctagatatga
      841 actaggtcag actactagat caaaaagagc tccaagttac gtaacaatta gtaatactaa
      901 agatatattc cgattgcaca tccttccaat gtttggtgag tatgcaatga actatctaaa
      961 ttcaaatact gagataatat cagatgaatt aacaaaaaaa tcgaaagaat acgcaaatat
     1021 aaaaattatc aaaggatatg tccgttctat gtttgatatt gcagaaatac taaattatat
     1081 tgaatttaac cgaactacca aaattattca atgtatcact gcacctaaaa aaattgcttt
     1141 agaagaaaga aggatacggg aaggtaatca agcattatcg tcagaagagc ttattgcttg
     1201 gatagaagca gtaaacactg acttgaataa tcacttgctt actcttcatg actatactct
     1261 tttcatgctt accctatatc ttggtgatag aaaaagtgag acgtatgctt tgcaatggaa
     1321 atatatcgac tttgagaaac aaacggttcg ccttaaacat gctttggata aatatcaaaa
     1381 aaagaaattt acaaaaggtc gaaaggatac cgtaattcaa gtacccgaaa ttgtaatggc
     1441 attactaagc aaatggaaat cagtgcaagc tgagcaattg ttgagattaa aaattaatca
     1501 aacaccagat cagtatctct ttacctatac taaaccatcc ggagaagtaa attgccctgt
     1561 tcacgctgat tacctaaact atcgaatcaa ttcaattaaa agacgacatc ctgaattggc
     1621 tcatttatct ccacataagc ttcgtcatac ctatgcaact atagctagac aaggcggagc
     1681 aaatatgaat caaatatcta gtgctttaac acactcagat atttctacaa cgagaatata
     1741 cgtaaataca ccagatgtag tagataaaac tgtttttgaa gcctttcaga gaggattgaa
     1801 taaatgtgat tagtatgtgt tcttaaatgt gttcttaaac aagaaaaaga cacttttcaa
     1861 acaattgaaa agtgccttga aacattgata tataaataat attaacgttt agagaattga
     1921 gatgctttac gagccttttt gagacctggt tttttacgtt cgaccatacg tgcgtcacga
     1981 gtaagtaagc cagcacgttt aagagctaaa cggaagtcag gatcaacttc tagcaatgca
     2041 cgagcgatac catgacggat agctcctgat tgtccagcgt atcctccgcc gtttacgtta
     2101 acaaatacgt catatgcgcc ttttgtttca gtaacagcga aaggttgatt gattacttca
     2161 cgcaagtcag catgtggaat gtattcttca acatcttttt tatttacagt aattttgcca
     2221 gttcctggta ctagacgtac gcgagcaact gcgtttttac gacggcctgt gccgatatat
     2281 tgaacttgtg ccaatgaaat tccctcctat tagattaagt ttgtgatgtc taaaacttct
     2341 ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata cgttcaattt tgtaaattgt
     2401 ttgcggccta aagtgttttt aggaagcata ccttttacag aagtttcgat caaacgacga
     2461 gaatttttag cacgcaattc gccagcagtg attgatttca atcctcctgg gtagttgctg
     2521 tgacggtaat aaattttgtc agtcgctttt ttacctgtta atttcacttt gtctgcattg
     2581 atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt atttttacca
     2641 cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt tgcgtctact
     2701 acataccatt tacgttctac ttcgcctggt ttggccatat atgttgtacg cacgttattt
     2761 tcctccaatt taagttctga tgtttgacat acacttcgag tttccggggc tctttgtggg
     2821 gcaaacaata ccatttaaca tattacccgc taatctagtc aatgtcaacg tttttctgct
     2881 tgattgaaaa atttatatga aactttactt cttttctttc ttttatataa aagggtcgtt
     2941 tcctgtcaaa atgaaaaaca aaaagaggct gtgacaaaag tcttgtcaca accccttatt
     3001 ttgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccataaaaca
     3061 taagaagctg ttttttaaaa ttgtttcttg tgactcggag ctgaacggca ggcttacaac
     3121 ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat
     3181 atgaggaact attttgacgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
     3241 cctctttttc ggtcaagttc aacaacagtt tttacgcgtc gatctctggc gctttgccca
     3301 aatgagcttg gatcatccag atacgttttt ctgtggctgt tttgaattcg ataaagatat
     3361 cttgtgttgg atcgtcgcct tcttctccag aaacttcgat tcctttttgg taaagatctg
     3421 ctagataacg atagcctgca acaagtgttt ccaaacgttc tggaatagta cgatcccagc
     3481 ggccaatttc atcagggatt tttgtgttat ctgcaaattc acgcaatgta gaataaggtg
     3541 aaccgtctaa tgtgatcaag cgttcggata cttcatctaa ctgagcgttc aaatcatcca
     3601 tgaactcatc catcattggg tgcaatgtaa ggaaacctgg tcctctcatg taccaatgtg
     3661 tttggtgaac aacaacagac atttggctta gatctgcaac taattgattt aatacttctt
     3721 tggtttgttg gaatttcatc tttcttgcct cctaacattt tcttacactt aaaataatag
     3781 tagactttca atgaaaagta caacaaaatc atttacctga gaacgatttt cagttcgctt
     3841 ttttatccgt aaagccaatc atttttcaga atcagctaat tcttctcgca gttgagcaaa
     3901 ttgctgataa gtctgtgcta aatgcttttg tgtcgaaaca aataatggat agattttctg
     3961 atatttttta tgatcagttt cttgtactcc aacttgctct ttcaattcta tcaattcctg
     4021 tgcttcactg atactttgga tctttccgat gcttctccag cctaaaatca cagctcctaa
     4081 tgcggatgct tctgtgacag tagggaaaac aagcgggtaa cccagtacat cagccatcat
     4141 ctgacgaaaa atcggagatt gagcaaaccc gcctgtagca cggatctctg tgagctgatt
     4201 ttcctggcaa actcctttta aaatccgata aaggttgaga cagatccctt ctagaatcgc
     4261 cttcatcatt tctttttctg tatgttgccg ctgtaaaccg ataaaactgc caaacgcatc
     4321 tgcgtcccaa agcggtgctc gctcaccaag taaataggga tgaaaaatca gaccattggc
     4381 acccgctggc acaaaagcga tagccgaaag cagtgcatca tatggatttt ctcctttttc
     4441 tttagcagat tgaacgactt cctgtaaaaa tgtatgccct gcccaatcaa agatgccagc
     4501 accattgcta gtcgctcctc ccactaccca atgtttctga tccagaacat aacaaaacgt
     4561 ctccatttct ggatggaact gaggctgagt agtaataaaa cgaagtgctc cgctcgttcc
     4621 tacggtcaaa gcggcgatcg tatcaccaaa agcacctaac cctagattag aaagcggccc
     4681 gtctgcccct cccaaaacaa atgatatatc atttggcaaa cctagttctg ctgctgtttt
     4741 tgatggtaga cctgttactt gataggtgga aggaaagact tgcggcaatt gctctattcg
     4801 aatgttcaag tagtcaagca cttcttctgc ccattggaat tcatggatat caaaatatcc
     4861 agttcctgaa gctgttccgt agtcgctgat cagttcgcca aacaatctat aaaagatata
     4921 gtctttgata ccgacaaaat aagcagtttt tttgaataat tcaggttggc tgttttgcag
     4981 ccacttgatt ttgttcaatg gtgtcattgg atgaatcggc gtcccagtct gccgataaaa
     5041 ttctttgcca agcacggtat atttcagttt ttctgcttcg tccgctgctc ggttatctgc
     5101 ccaagtgatc attcgagata acggtgtttt ctcttcatcc aacaatatca ggccatgcat
     5161 cgcagaggag aaggagacaa attgaatgat tgtgtctgaa ggcaactgtt taattacttc
     5221 ttttataacg gtgcagacac cttgaaagat caattcctga tcttgttctg ccatcccttg
     5281 tgtttcttgg atcaaagggt agcgaaaata agcggatgct gcttcttggg cttcatctgt
     5341 gaaagcaact gctttggttt gtgtggttcc gatatctaat ccgattgaaa cgttcatttt
     5401 ttcacctcaa attttatgta tgtacaaaaa tttagtttaa gcttgcgtat cttggttttg
     5461 aaaaggggga gatggcgtgt tactttattt tatcattggt tgctgtatcg gatcattttt
     5521 atgtttaacc gcccagcgga ttccgttagg tcactctatt atttatccta gatctcattg
     5581 tgtcaaatgc tgtcgttcgc tttcttggta tgaattgatt ccgattctct cgatcatcgt
     5641 ccaacgattt cgctgccgct attgcaggtg tcggttgccc ttctactatt tgttggcaga
     5701 agccttatgt ggtggtcttt ttgcctggtt ctttactttt tcttccgcaa gaaatttttc
     5761 gacgttcata tggatgcttt cggctttgct attttcgttg atggatttat tttacttcat
     5821 ggtccattct cacacgctat tctgttcttg ggcaatttta tggatttttt ggttgcaggc
     5881 cggcgtcttc caatggcaaa gtgtgctgtt aacgtttatc gtaggcggtg tgtgccttcg
     5941 ctatggtcaa tcctatctcg gagctggcga tactctgttg atgctttctt ggagtggtgg
     6001 cttgtctcta gaagagttgc ttcagatctt gtttcttgct agtctgtttg gaatatccgt
     6061 tttttctgtt tactttatct gtcataaaaa aaagttggaa aagctgcctt tcattccttt
     6121 tttgagcagt gcgttgctga ttgtccttca tcttaggtat tgaaaaaagg ttgtgacaaa
     6181 cgacttgtca caacctctga tatatagtgt tctcttacca aatccttgtg acttggagct
     6241 aggcgacagg ttcacaacct ctccactaaa tggtgttcaa tcagctgacc ctcagtatta
     6301 gctcaaaatg ctggttttac aacctctgat atacggtgtt ccataagtag ctttttcggc
     6361 ttcttatttc ttgacgcccc aatgtcgacg aaggaaacct gcacgtccag aaccttctcg
     6421 ataaagattg taatggagct tgtttttctt gtaataatcc tgatggtaat cttctgcagg
     6481 ataaaattcg cttcttggtt cgatttttgt cacgattggc ttatcgaatc gcccgctgtc
     6541 ttgcaatgct tgttttgaac gctctgcaat atttcgttct tcttcatcat tatagtagat
     6601 cactggacga taagaatcac cgcgatcagc gaactgtcct aatgcgtcag tcggatcggt
     6661 ttgctgccaa tagatttcca ccaactgatc ataagaaata acttctggat catattcgat
     6721 ctctactgct tctgtatgcc ctgttgtgcc tgttgttact tgttcataag ttggatttgg
     6781 tacatgcccg ccagtataac cggatgtcac agaaatgatc cctggctgct cttcaaatgg
     6841 tttgaccata caccaaaagc atccgcctgc gaaaactgct ttttttgtca tataaaatcc
     6901 cccttttttc tcatcatacc ttgatttcca aaaggaggat aacgttttgc ttgtgacaga
     6961 aaaaacggaa actattatat acgtgatacg gcttctgata aatgccaaaa cgaacgtact
     7021 tgatcaaaaa cggttaaatc aggaatcaca tattcagcag aaggaagatt tatgttttca
     7081 cctattccca atacatgcat gttaccagcc aaagctgctt gacaaccagc ttctgaatct
     7141 tcaatgacca agcatgcatt tggcggtaca ttcaattgct gtgctccttt gagaaaaact
     7201 tctggatcag gttttgcttt tgatacttgc gtgccgtcaa taaggacatc aaaataactt
     7261 gtcaaattcg ttttttctaa aatgaggcgt gcatttttgc ttgctgatcc caatccgatc
     7321 ttgatgtttg tttttttcaa gtactcaaga acagctattg caccaggcaa aacggatgtt
     7381 tcatccattt tttgaatcgc ttgaagatat agattgtttt tttgctcagc aaaggctgat
     7441 ttctcttttt ctgagaattt ctgttccatt tttccaaatg acagcaatcg ctctagtgac
     7501 tcggttctgg atattccttt cagttgctcg ttttctttct cagtgaatgg aatggacagc
     7561 tggttagcta aaaccagcca cgcttcgtaa tgataacgag ctgtgtctac aagaacgcca
     7621 tctaaatcga atagtgctgc tttgattttc atcgattacc tccttgaaaa tgaacgactt
     7681 gaagtaaaga tgcgatgttt ttagctgtgc gatgaagatt atcagtggct tgctgtaaag
     7741 cttcttgtaa aggcacagct gcaggaagga tcggaaaaac ggccgaaatg ccaacatctg
     7801 ctgctttttc gcttccttta ccaacacttc cgcaaatagc tatcacagga acgccctctg
     7861 gtactcttct agcaatccca actggcgctt ttccttttag actttgtcca tctagacgtc
     7921 cttcgcctac gattacgagg tctgcttgct gtacccgttt atcgaactcc agtaaatcca
     7981 atacgtagtc gatgccagag actattttcc ctgaagcaaa tgctaacagt cctgcaccca
     8041 ttccaccacc agcactactg cccgatgcct cgattacttg aggggcaaat ctcatataaa
     8101 aatcatgcat ggcttggtca gccactgcta attcattttc tttcaagcct ttttgtggtc
     8161 cgaaaacaaa agaagcgcct tcttttccgc ataaaagatt agtaacatca gtaatgacag
     8221 tgattttggc tttaatcagc tcaataggca cgttcgtttt atcaactgta gcaattttac
     8281 ctagattttc tcccagtgct tctacttttt gattatcttg atcgtaaaaa gcataaccta
     8341 gaccttctgc catgccgatg ccgccatcgt tggtactact tccgccaacg ccgatgatga
     8401 tgtgtcgaac accttgttgc aacaattgtc gtatcagttc tcctactcct cgcgtggata
     8461 acctaagtgg ctgttgttgg tttagtggga catctttcaa tccgacgatt tctgccattt
     8521 caaaaatcgc agtttgaccg tctgaagcgt actggatttc tttgtttttg ccaaatgcat
     8581 tagtaacgac tgctgtttgc cgcttcaacc ttaaactgtc tgctaatgat gaaaccgttc
     8641 cttctcctcc atcgcctata ggtagcagat catattcagc ctgaacaaaa acttcttgaa
     8701 aacctgattg aatggcttgt gctgcctcta aggctgtcag actttctttg aaagaatctg
     8761 gcgcaatcag aatacgcatt attttcccct ccgttccaag atcagttctt tttccagctg
     8821 gtattggtct ccattgacaa aaataggtaa tgtttctttc gtctcttttt ttattactat
     8881 ttgttttttt gtcaaaacaa atagcaacct gtgatttttc caaataaaag gaaaagaaat
     8941 tttttcccat gctgaaggaa gtcttggaga aatagaaaga gttccattcg ttactgtaat
     9001 acctgaaaaa ccaaatataa ctgctagcca tagtgctcct aatgaagcag catgaattcc
     9061 gtcatctgat gaatgcggat ctggtcctaa atcaatcagg cacgcttctt ggaataaatg
     9121 atacgcccat tccatttctt ggatacggca agcctctatg gcgtgaatcg ctttactcaa
     9181 cgatgagtcg tggattgttt tgttttcata gtaatgcaaa ttcttagaaa cgattggttg
     9241 agaaaaatac tcaggtaata agtataacag catgacaaga tctgcttgct tgctgacttg
     9301 gagttcattt acttcgctac gggcataatc aagtaaaata gactggctgc caggacgttc
     9361 tttgtaagtg gtcaaatcaa taactggctt actgagatac gtgtcatctt gcggaatcag
     9421 ctgttctttg ttaggcactg gcaaatataa cttatctaaa aaattttgac attctttcaa
     9481 aaaaagcgga tcgccagctt gatatttaac agctaaggcc acattataat gcgccagata
     9541 gttcgtatat gcattattgt cgatatattc tgtgtattca tctggaccaa tgactctgtg
     9601 tatctctaaa tgatcatcga cttttacagc tctgctcagc caaaaagctg ctgtttcatg
     9661 aatgagcgtt tttccctttg tttctaaaaa cgattgatcc tctgttgctt ggacatactc
     9721 ttgaacggca taagcaaggt ctgcactgat atgatgttct gcggcagctg aagcaacttc
     9781 ttgtcttgtt ccggttttga tattcaatgc agcaaatttg ggtgtctctt cctgaccagt
     9841 gtgtgcgctt tcccatggga aaagtgcacc ttgatagtga taatctcttg ctttttgccg
     9901 tgcgccttct aatcgatcat aacggtacag cagcaaattc tttgctactt caggaaactg
     9961 atataaaaag aaaggcagga taaaaatttc cgtatcccag aaaacatgcc ctttataacc
    10021 ttcgccagtt agtcccttcg cccctacact aacacttgga tcattgcggg gagtcataat
    10081 ctgtaaatgg taagcagcga agtccatcgc taactgatcg aaagaagaat cgccttggat
    10141 cgttatccga tgctgatgcc aaaaatctgt ccaataagac atcgactgct ctaaaatttc
    10201 tgtataacta acgcttttct gggcttgggc aatacttttc ttaaacgggt ctgtttgctg
    10261 aagagaggta gagagcacga tgatcttctc cagcgtaaag gacgtatttt ttctgatctt
    10321 ttctgaaaag gaagcggtga tccggcgatt ttttgcttgg aatacacctg ctttattgaa
    10381 tttagctgca atcccaatat caattttact ttctgttgtc tggtaggatg cactgatcaa
    10441 atcgtcacca aatactcgca gttctttttc aaccaactgc tgtgtaccaa aattggtttg
    10501 ctgtccatcg atacccgttt tcacatctac ttggcagtct tcggtcaacg gcttgatgac
    10561 cactttcagg gctgcctgat ggagattatt ttgatcagca acacgacaag taatgacttc
    10621 cacttttttt ccttgagcag tttcaatgag cgcatagcgt ttaagctctc ccatttctaa
    10681 atctagtgcc cgctcgtatg caagaacttt catcgtctca agagaaaaat gttcaccgtc
    10741 gatttttatt tcccatctga tgacatctgg gagattcact aactcggctg attctgctcc
    10801 taaaggtcgg ttatatacac cagctgcaaa cattcccctt acttgattcc gatagtcttc
    10861 ctcctggctt cctctgatac caaaaaatcc atttccaatg gtcatctgac tcgcatactt
    10921 attgatatac tctaaatcaa actgtttttc ttctactttc ataaggtaat aacttctcct
    10981 gtttctgccg aacgatataa attttccacc agattctgga tgacatatcc ttgccagcca
    11041 tctgcaatca tctgagtctg gtcaccgagc gcccgattca caaaatgagc catacttagt
    11101 tcatgtgggt cattaactgg cgtggtcttt tgtaaggaaa ccagctttcc atctacatct
    11161 tcgaatagtt cgcctggata cagattagca cctgcctgat cgccacaaaa agccacattc
    11221 agtttgtctt ctctgatatt caaagcaaac gaagtattca gttggatgat cttgccgttt
    11281 tctaattcca gtgtgccaaa aattgcatct tctactgtga atttgcttgg atcccatttc
    11341 ccgaatttcc ctgaattttt tttcgtacca attttttgga agctgtacgc ccgaacgctt
    11401 tttactttag gaaatccagt aatatacatc gctgtatcaa gcatgtgaat gccataatcg
    11461 atcaaaggcc cgcctccttg aagttctttg tcgataaacg ttccccagcc tggaacacca
    11521 gaccgacgaa tcgcattcgc ttctacgtaa taaatatccc cgagaacatc aagatgattc
    11581 ttgatcattg ttgcttcgtc agaaaaacga tgctggaatc cataagccaa aatctgcccg
    11641 tttttatcgg ctgtctctgc catctttctt gcttgatcag ctgtcatagc tggtggtttc
    11701 tcgcacatca ctgaagcatt agcttcgagg gcagccaata catgttcgaa gtgaaagcga
    11761 ttgggtgtac agacactcac gatatcggga gaaatctcat tcagcatttg tgtaactgat
    11821 gtatacgcat gagaaaatcc tgtacgtcgg caaaagtctt ctgctcgctg gctgactgga
    11881 tcaaccacag cggccaattc taagacatct gatctttttt gataataagc agcatgtact
    11941 ttttctgcta cttgtccagc gcctatgatt gctactttct tcatttattt ctccctcctt
    12001 tgcttattta cttattgtta ttctttctct attctttctc taaatcattt aaatgacgcg
    12061 ctgtttccag acagtttttc aagtattgca atgattcctg ataaattttc acttcatttt
    12121 ctcctcgcaa acgacattca tataccatat aaccttcata ctcattttga agtagcgttt
    12181 gaaaatggcg agtaaaatca attgcgccag atcctggttg gtagcgatga ttatctgcta
    12241 aatggatatg acccacatac tctttttgcg tagtcaatgc ttttgaaata tcatcttctt
    12301 caatattcat atgataaaaa tcagcgatga tctgcacatg tttcaattga ttagagtcaa
    12361 tgtaatgacg cgcatccgct aacaaattga tcatatggtc ttggtatcgg ttcaaaggct
    12421 ccaaataaat aaccgtacct gtttcttcgg ctactttatc taaataacga agtgattcgc
    12481 tgactgcttt atagtctcct tctttgcttc ggggtgaaac catcggcggt aatcgataag
    12541 tgaacattcc ccaagctgct gggataacga ttcctttgcc tccaacctcg cttagtgcac
    12601 gcaaaatctc agcaatttga cgtacaccat taagacgccg ttcttcgata aagtcaccga
    12661 tccacccatc gtagccgtta caagctgtca ctactgggat ccctgtttct ttgattgcct
    12721 gtttgactgt gtcgatattt tctattaaca acttaccgtc gatttcgtat tcttcaaatc
    12781 cacagtcttt gataaatcta aatttttcta aaatgtctct tgggaaaaat gcttgatctt
    12841 gcgtaccgaa tttcattgtt ctcctcctcg gacttgaacg cccattttga tactttcttc
    12901 tgggtgctgg tctacataac gcatgtaagc ttctgcgctt tctgcaaatg gcacaactgg
    12961 atcaatgatt tcttcacaat ccaagtaacc gttcatcaat aattcccaac atgtgtcctc
    13021 gattcgttta cggttccatc ttgggtaatc tgggtttggt tcactagatg ctcgagcaaa
    13081 gacgattttt gcattattga aatgtgcttc gcgaccaaga ttaaacccag ctggaaaagg
    13141 tttagcaaag gcaacataag aaatgatccc gccatagccg atccctctta aagaagcttg
    13201 taatgcggca gcactgccac tcgtttcaat aactgaatct actccacgtt tatctgtcaa
    13261 ttcctttaga cgaaaaccga catctgcttt cataggatct agcgtgtaat cggcaccgta
    13321 tttttctgct aattctctac gctgcgctat gggatcgatc ccaacgatgg tagtagcacc
    13381 tgctttttta gctaattgaa tcgcaatttg cccgatagcg cctaaaccga agattgctac
    13441 agcatcacct gctcgaacat gtgcttcccg gataccacct aaagcgaatt gagcaggatc
    13501 gtaacatact gcatttttcc attgatccaa ccgatcaagt ttacgtagtt tataattatc
    13561 aacaccgttt gtaatcagcg tttcacgaat gggtccataa gtgcagacta actctccttt
    13621 ttgatactct gtcaccaaac tgcccgtttc ttcgatcgta ccgacaatca tatttcctaa
    13681 ttgaaattcg ccaaatacca caccgcgttg gcttccttct tctcttggca agaataggcg
    13741 ccattcttct gaaaattctt catcgataaa tggacttaat cctctaaagt cgacaatttc
    13801 tgttcctggt tttggagtag cgaaaattac tcggatacgt acttcgttct tttcgatctc
    13861 acggtcttcg taatctttga atccggctat tcttggttct aatgcaacta attttttcat
    13921 cttttctttc ctctttcttt tctttttatc cttttacgcc gccttcgact aaatcacctt
    13981 taataaatcg ttcagataac gcatacataa tgatgactgg taaagcggtc accaatgaag
    14041 cggccatcat tcgtccccaa atgtagtctg gtgtactgaa taatgtgttc aaaccgattg
    14101 gtaaagtaaa tttttcggag gtcgacagga aaatcgaagc aaataaataa tcattccacg
    14161 aaaccataaa gcaataaacg aagacagaaa tgattccaga aatagccaat ggtataatga
    14221 tccgaataat gatctggatc cggctaagac catcgatcat tgctgcttct tcaatatcag
    14281 tcggaatcgt gtcaaaataa ctttttagca tgaatatcgc agtaggaagt gtttgaacga
    14341 ccatcgtgat aatcagtgct gtattggtat catacaaacc tagaccggaa atgattttaa
    14401 ataatggaac aacgatcaaa ataccggaaa acatataaac ggtgtaaaag ctagcattga
    14461 ttttcgagcg agctttgaag cgtagttttg acaaagcgta tgccccgctg atcccaatca
    14521 ctactgcagt tacggaggct atcaaggaga tcactaaact atttttgaaa tacatgatga
    14581 atggaaaaat ctcagcattg aaaatatcct gataatgttc aaaagtccat tctttcggaa
    14641 aaatagtttg tgtcgttgca attgcttctt gtgaatcctt gaatgatgtc atcagcatga
    14701 tcaaaaatgg aaacaaattg acagctaata gcaacaaaat cgtcgcgtaa aataagattt
    14761 tcctgctctt tttatttttc atttcaagtc caccttcttt cgtgagagca agacgaccgc
    14821 tgcaataatg atgaacaata gaatcgatat agctgctgct tttccaagat cattgaacgc
    14881 aaaggctgtc tgatacaaat aaacgcctaa gatattgact ttattcgtca gtagatagac
    14941 atctgtaaac atatagaaca tccagataga tcgtaaaaca atcacagttg ctagtactgg
    15001 tttgattgcc ggcagtgtca cgatcttgaa cttctgccaa gcatttgcac cgtccatttc
    15061 agctgcttca taaagcgttt tgtcgactgt ttgcaaaata gccaaaaatg aaataaacgc
    15121 atacggatag tatctccaga tagcaaacaa aacgaccata aagaagctag caactggctg
    15181 gtcgaaccac agcggtgctt cgttgaaaag ctgcaaccta tctactaaca gatcattgac
    15241 gattccataa ctattattga acatatattt ccaagcaaaa atcaaagaaa tagatggcgt
    15301 aacataactt aataaaatca gtgaacgcac cgttttccta aatctgaact tgcggttaag
    15361 caaaatagct acggctaatc ctaaagctgt actgcctgct acaactaaga atgtgtaagc
    15421 aactgttaaa cctaaagaac gataaaattc actatcactt aaaatttcaa gataattgct
    15481 taatccgata aaaacggcgt ctgagttagg attcagcggc atatcgaaaa aactgatctg
    15541 aatattggaa agcattggaa tcagcactaa aacagctaat aaaagcatgc ttggtgctaa
    15601 aagtcccatg gctaatttta agtcagaatg tttccttgga cgattcattg aaacctcccc
    15661 ctgataatct gccaatagct atctactttt ttcgaacacc attggcagaa cttcttgtta
    15721 tttcgtattt ttttgtgcgt cagctaaagc ttgatcgaca tcttcgtttc cgacagttac
    15781 ttggttcact gcttttccga tcacacctga gcttgtgata tctcccattt tcgtgaaatt
    15841 cttttccccg actagtccaa agacttgtac ttcatcaaaa gcactagcaa tctcagatgg
    15901 cagctcgcca aaagacttga tgacttcatt ttctttgtaa ccatccagtt cgactacttg
    15961 tttattcact ggttgagcac caccaggaga catcaagacc cacttttcca tgtttttcgg
    16021 ttgagataag aaagctgtga atgcttcagc agcctctttt tgtttctcgt ctagaccaga
    16081 agaaatcgtc aatccagata ctgtgccata gacagcttga tctttttgtg ttgggatagc
    16141 aaaaccgata ttttctgagt tccccgcttc ataaacagaa ggcaaaatgt aagttgaata
    16201 gattgccatt ggtgctgttc cattcatgaa agcatctttg atttctgtca catcattaga
    16261 gcctggcatc gtgtattggg ataagttttg ataaaacgac aatgcttctc tcatcttttc
    16321 cgtatcgatc gttacctcgc cttcatcatc caaaacattt gctccatttg acaaagcaaa
    16381 ttgagagaac gcttgctcac tcattgttcc ttcagcagta ggcatagcaa ttccatattg
    16441 tttattctct ttatctgtga aatgttgagc aatttttaaa atatcatccc aattttctgg
    16501 ttctgaaaaa ccagcttctg ataatttttc tttgtcatac caaatacctt gaacccagcc
    16561 gctaattggt gcagcaatat agctttttcc atcttctgaa cgaacaaggt ttttggctcc
    16621 gtcataataa ttatcttctc cagcttcctc gataacgttt tggactgccg tctgatcaat
    16681 cagttcatct ttatccatca ctttagcaaa atcctgactg acttccatta cttctggtaa
    16741 tttaccggaa cgagctaacg taacgatttt tgtattgaac gctgattctt ctaccggcac
    16801 ttgcttgatt ttgatgtctg gattttcttt ttcaaaatca gcaaccagtt cattgatcac
    16861 acttaatcgt tcctgttcga cagaagagtg catgaattga atcgttgtct gttgtgattc
    16921 gttagagctt tgactttcac cacaagcagt aagaagaaat ccagtagcta gcagacaggt
    16981 agtaacaata atttttttca tttttttcca cctcttattt tattattttc agccacatat
    17041 attcatacgg ctggaatatc aactttcgtt cttgactgca atggataatt tgtccagaaa
    17101 acaggtctag gtaatctcct tttggtaaaa gataacctgc tgattgattg gaaaaattat
    17161 ggataactaa gactttctct tccttcgctt cgcgaataaa actgataaaa gaatctgctg
    17221 agtccagtaa gtaaatcaaa ctattcggat ggaaggcttt ttcttttttt cgaatagata
    17281 gcatctgggt caattcacca tatactgctg ttcttaatgg atctgtctgc aactctttct
    17341 caatatctgc taacaaaaat ttttgccgat tgatttttcg attctctcct gttttttcta
    17401 cgcctttgta atcatttcga cttcccagca cactttgaat ataaattgcc ggcacgcctg
    17461 gtaacgttac caaaaatgaa tgagcagcta aaaagcgttt gcttctccat tccgtcgtat
    17521 ctgttggaga acttaacgca tctagatacg tagcgtttat ctcatatgga ctggtcgttc
    17581 catctgggtt tttcttgtag tttactcggg caccctcttt cttcagctgt tccactaatt
    17641 caaggatcgt tttttccggc agaatacctc gtaccggatt caatccgata ccgtcatggg
    17701 aggctaaaaa attaaaaaaa gttgtttttt tcgatgcgaa atcgatggtt tttaaccatt
    17761 ctttcaactt acttgtatta cccgaacgaa tcgcttgaag ggtcaaaggt ggtagcgaaa
    17821 actgatagac catctgtgct tcattctcgc cattcccaaa ataactgata ttatctttgt
    17881 gtggcacatt tgtctcagtg atcaataccg ttcctggagc tgcttcatca gcgattgcac
    17941 ggaataattg gatgatttta tgcgtttctg gcaggtgtat acaagaagtt cctagttctt
    18001 tccataaaaa tcccacagcg tccaagcgaa taaatgaagc gcctttttct aagtaaaaca
    18061 aaagaatatc gatcattttc aataaaactt ctggattttt aaaattcaag tctacttgat
    18121 cttcgctaaa agtcgtccat acgtgttttt ctttcccgtt tttgtcaatg aacgttgtaa
    18181 gtaacgggct ggtccttggt ctagtcacgc tgcttgtatc tgtatccagt ggcaaatcaa
    18241 taaaaaaatc tttgtacgta tcattcaagt ttaaatattc ctgaaaccaa gtactttcgg
    18301 cagacatatg attgcagaca aagtcgaaca ttaaccgtgt ttcgcttctc atttcttcta
    18361 tatcttccca tgtgcccaaa cggtcgttga ttgcctgata atttacaacg gagaaaccat
    18421 catcagatgt atatgggaaa aatggcaaga aatgtacaac tctaaatgta tctgccaaat
    18481 acttttgata aaatttctta aatgtacgca aagttggttg ctgctcttca aaaaattgat
    18541 ccccataagt aatcaagaaa atatcttctt cgtcccaatg cagtttgcct gcctggcctt
    18601 tcttctgctg ataaacactg actttttgtt caatcataga ggcggttttc tcagacaaat
    18661 ctgagtcgcg ataaatcgct tgtaaaagtt ttgtatacat cggattcccc tttctttttt
    18721 gggagcgttc ccaacgattt gatcatatta tggaaaacgc ttactgtcaa tagcattttt
    18781 ttaaataatt ttgatataca caaaaataca aataagaaag cttatgtatc aataattaat
    18841 agcttcttaa aaaaatatgg tctctcatga tcatgcaagc tattcctaaa ttttcaaaat
    18901 catagacaaa aaagaatgtc tattgtacta tggaattatt ctaaaacgct cccaaaaagg
    18961 aggtgcatat aaaattgaaa cctactattt atgacattgc gaaaaaagct aatgtctcaa
    19021 aatcaactgt ctccagagta ttaaataacc agccaaatat ctctgataaa gcaaaagcaa
    19081 gtgtacttca agcaatcaaa gaattgaatt atgaaccgag caaaattgca agaggactgt
    19141 caggaaatgg ttttgacgcg attctagcat tatctcatcg tacttctcat acgacctatg
    19201 gcaatccttt tttttcagat gttattcaat ccatttctgc cgtttctgaa caaaataact
    19261 tcgacttgat tctacaaacg gcaaaaacac cagaagaaga gctagaaaaa tgcgcgaaaa
    19321 agatccaaga aaaaatgatc cgaggaatta ttattttaag ctctacgatg gatgaaacac
    19381 ttctttttga attggatcaa tacaatattc ctattgtggt aatcggaaaa gtcggaaacg
    19441 actacacgaa catctactca gtggacacgg ataatttcca ggatagttac gatttggtgg
    19501 aaacactgat caagaatgga caccgtcata tcggctgtct ccatgctccg acagaagttt
    19561 atgtatccaa tgatcgtctc aatggttacc gtcaatgctt atttgatcac cacctagata
    19621 tccgtaatga atggattata gacggcggct ttcgcttaga agatagcatg gcagcaatga
    19681 atgatttaat gaagctacga gaaagaccaa ctgcgatttt ttctaccgat gcattaaaat
    19741 cattgagtat ttacaaagtg gcagaacaat ctggaaagca gatacctgat gatctttcga
    19801 tcgcaggatt cagtgatccc ttattgtcct ccctattttc gccacagttg actcggatag
    19861 acattccgac aaatcagtta ggaatcaaag ccacagaact tttattccaa ctaatccacc
    19921 accaataccc tgaagaaaaa agaatcattc tttcaaccga gattactctc acagattcga
    19981 tccaacaaca agaaatgtag aacagaatta taaaagaaca gaaacactga ggataagtca
    20041 gttactctgt tcttttttca atcactcatc cctcgttacc ccctcccggt attttagcga
    20101 atttttctta ttttccatct gttttagata gctgtggata actataatgt ttgtgttaaa
    20161 ctattttttc cacagggaag aaaaaactat ttttaaccac gttgtgtata agtttttata
    20221 gttcgttttt ttaaacacat ttacccttat ttcaatccat gttatttggg gatacttatc
    20281 tctaaaacat aaatagaata acagacatgt ctactggttt tgttttattc gaggaattat
    20341 tcaataatag ctagcaaaat attaaaaata accgctctat cttcccatca atttattttg
    20401 cattcaaaaa atgatctatc tttacttttg ttgattgact tctcttggaa gatgatcgtt
    20461 ccgatacctt aattggatac gaaccgatgt tgttaagatc ctttcccact tttcttctag
    20521 ttagacaaat tattttagac aaaccaaaaa agaggttgtg acaataattt gtcacaacct
    20581 cttttttaaa atatctattt tgtttgattg ttcattgcgt cttctgcagc catttgcgct
    20641 tcaatatctg aaatactgta gacattttcg cttgctacac cgacttcttt tggttccatg
    20701 tcacgataac gttgcatacc agtaccagct gggatgatct tacctataat aacattttct
    20761 ttcaaaccaa gaagaggatc tttcttaccg cggatcgcag catccgtcaa gacacgagtt
    20821 gtttcttgga atgatgcagc agataagaaa ctatttgttt ctaatgatgc ttttgtgata
    20881 cctagtaaga caggtcgaga agttgcagga acgcctcctg agactagcgt attgtagttt
    20941 tgatctttaa agtctgcgat gtctaataat gtacctggta agatatctgt atcacctgga
    21001 tccattacac gaactttacg cagcatttga cgaaccatta cttcgatatg cttgtcgccg
    21061 atttctaccc cttgcatacg gtaaacacgt tgtacttcac gaagcaagta gttttcaaca
    21121 gataatacat cgcgaacttg caacaattgt tttggatcga ttgatccttc tgtcaatggt
    21181 gcaccacggt ggatcatatc gccttctgct actttcatac gtgctgtata aggaacagta
    21241 tacgtacgtg tatctgtttt tcctttgatt gttacttctt tggtacgtgt tgcaggatct
    21301 tctgagatat cgatgacatc ccctgttact tctgtgatca ctgcttgccc tttcggatta
    21361 cgtgcttcaa agatttcttg gacacgagga agaccttgag tgatatcgtc tccggcaacc
    21421 ccacctgtat ggaatgtacg catcgtcaac tgagtaccag gttcaccgat tgattgagcg
    21481 gcgatcgtac caactgcttc tccgacttca acgtcagagc cagttgcaag gttgcgtccg
    21541 taacaatgtt tgcagacacc atgttttgta ttacatgtga agacagaacg gattgtcact
    21601 gtttcgacac ctgcatcaac gatttgtttt gcgatgtctt cggaaatcaa ttggtttcct
    21661 gcaatgatca attcatttgt ttcaggatga cgtacttctt tacgggtgta acgtcccact
    21721 aaacgatctt ctaatgattc gatgatttca tttccctcgc ggatggcttg gatttcaaga
    21781 cctcggtcag taccgcaatc ttcttcacgg atgattacat cttgggccac gtcaaccaga
    21841 cgacgagtca agtaacctga atcggcagtc ttcaaggctg tatcggtcat ccctttacga
    21901 gccccgtgag ttgagataaa catttccaag acggatagtc cttcacggaa gttcgaaatg
    21961 attggcaatt ccatgattcg accattagga gcggccatca aaccacgcat tccggcaagc
    22021 tgtgtaaagt tggaaatgtt accacgagct ccagaatcag acatcatgaa gattggattt
    22081 ttcgcttcca aaccttccat cagtttttgt tggatgctgt cttttgcagc attccaaaca
    22141 gcaatgacac gttcataacg ttcgtcatct gtgatcagac cacgacggaa ttgttttgta
    22201 atagtttcaa cttgtttatg ggcattttca atgatttctt gtttttcatt cagtacactg
    22261 atgtcagcga tccctactgt gataccggca tgtgtcgaat gtttgtaccc taagtctttc
    22321 atacggtcaa gcatcttaga agtttctgtt actttgaatc gtttgaagac ttctgcgatg
    22381 atgtttccta agtttttctt cttgaacggt aatactaatt cttgctcttt gatatgtgca
    22441 ggaatatcag taccagcttc aacaaagtat ttgtccggtg tttggacggt caagttgaag
    22501 tctgttggtt cattcagata agggaattca ggaggcatga tctcgttgaa gatgatctta
    22561 ccaactgttg tgatcatcgt acgttctttt tgccattcag taaatggttt ttcacctaat
    22621 gagttagggt tcacaccgat acgtgaatgt aaatgaacat atccgttgcg ccatgcgata
    22681 acggcttcgt tttgatctct gaagaccatt ccttcgcctt cgcgtccttc ttcttccatt
    22741 gtcaagtagt agttacctag aaccatatct tgagaaggag taacaactgg tttaccatct
    22801 ttagggttca agatgttttg agcagctaac atcaacatac gtgcttctgc ttgtgcttct
    22861 tcattcaatg gaacgtggac agccatttgg tctccatcga aatcggcatt ataagcttca
    22921 catacaagtg gatgcaaacg gattgcgcgt ccttgtacta aaactggttc gaatgcttgg
    22981 ataccaagtc ggtgaagcgt aggtgcccgg ttcagcaata ctgggtgttc cttgattacg
    23041 tcttctaaaa catcccatac ttcgtcttct tgacgttcga ttttacgttt cgcatttttg
    23101 atgtttgaag ctagttcgcg ttgaacaagc tcacgcatca cgaatggttt gaataattca
    23161 atggccattt cttttggaag tccacattgg tacatcttca agaaaggtcc aacaacgata
    23221 acggaacgac ctgagtagtc gacacgttta cctagcaagt tttgacggaa acgaccttgt
    23281 ttccctttca acatatgaga aagagatttc aatgggcggt tacctggtcc agtaactgga
    23341 cgtccgcgac gaccgttatc gatcaatgca tctactgctt cttgcagcat acgtttttca
    23401 ttttgaacga tgattccagg tgcattcaag tctaacaaac gtttcaaacg gttattacgg
    23461 ttgattacac gacggtaaag atcattcaag tcacttgttg caaaacgtcc accttctaat
    23521 tgaaccattg ggcgtaaatc tggtggaata acagggataa cgtccataac catccagctt
    23581 ggttgatttc ctgaagcgcg gaatgcttct aaaatatcca aacggcggat tgcacgtgta
    23641 cgtttttgtc cttgtgcaga tttcaattct tctttcaatt cggctgcttc tgcatctaga
    23701 tcaacattat caagtaattg tttaatcgct tctgcaccca tagcagcttg gaattcttgt
    23761 ccatattgtt cacgtttttc gcgatattcg cgttctgtca acaattgttt tttctctaaa
    23821 ttcgtatttc ctggttcgat gactacatat gaagcaaaat aaatgatttc ttccaaagcg
    23881 cgtgggctca tgtctaatac aagacccata cgtgaaggaa tccctttaaa gtaccagata
    23941 tgtgaaacag gagctgctaa ttcaatatgt cccatgcgtt cacggcgaac tttcgaacga
    24001 gtaacttcca ctccgcatcg atcacaaacg attcctttat aacggatacg tttgtatttt
    24061 ccgcaggcac attcccagtc ttttgtagga ccaaaaatcc gctcacagaa caacccttcg
    24121 cgttcaggtt ttaaggtacg atagttaatc gtctcaggtt tttttacttc accgtaagac
    24181 cagcttctga ttttttcggg agaagccaaa cctatttgca tactttcgaa tttatttaca
    24241 tcgatcaaaa ggggttccct ccatttcatt taatggcaaa ccgcaagacg gctgatcttc
    24301 gaccgccttg cagctcatag accggattag tctaaattat cttctgcggt ttcaaagttt
    24361 tggatgacat cgtctttttc atcttccact tgttcagcag ctttcttttc taattctttg
    24421 gcagtttgct gttcggcaaa ttttgtcaaa gcatcaacag tgatcaaatc gtcgtcttca
    24481 tcgtccatgt cgcgaagttc gatctctgtt tcttcgatat ccaagacacg catgtccaga
    24541 cctagtgatt gcaattcttt caccaatacg cggaatgatt ccggtacacc tggttttgga
    24601 attggttcac ccttgacgat tgcttcgtat gttttcacac gtccgactac gtcatctgat
    24661 ttgtatgtca agatttcttg taaagtataa gccgcaccgt aagcttccag tgcccatact
    24721 tccatttcac caaaacgttg tccaccaaat tgagctttac ctcccaatgg ttgttgtgta
    24781 accaatgagt aaggtccgat tgaacgtgca tgcaatttgt catcgaccat gtgagcaagt
    24841 ttgatcatgt acatcacacc gacagacaca cgtccatcaa atggttcacc tgtacgtccg
    24901 tcgtacagaa cagttttcgc atcgctggcc ataccggctt cacgaactgt ttcccatacg
    24961 tcttcatcac ttgcaccatc aaatactggc gttgctacgt ggatacctaa ctgacgtgca
    25021 gccataccca agtgtaattc caatacttgc ccgatgttca tacgtgaagg cacccctaaa
    25081 ggattcagca tgatatcgat tggtgttcca tcaggtagga atggcatatc ttcttccggc
    25141 ataatacggg atacaacccc tttatttccg tgacgaccgg ccattttatc cccttcatgg
    25201 attttacgtt tttgaacgat ataaacacga actaacatgt ttacgcctgg tgacaattca
    25261 tctccagctt cacgagtaaa gattttcaca tcgtgaacga taccgccgcc gccatgtggt
    25321 acacgtaatg atgtatcgcg gacttcacga gctttttcgc caaagatcgc atgcaataga
    25381 cgttcttctg cagataattc tgttacccct tttggcgtga ctttccctac aagcaaatcg
    25441 ccatcttgga cttctgcacc aatacggatg atacccattt cgtctaaatc ttttaatgcg
    25501 tcttccccga cattagggat ttcgcgtgtg atttcttcag gtcctaattt tgtatcgcgg
    25561 gcttctgatt catattcttc aatatggata gaagtgtata cgtcatcttt taccaaacga
    25621 cggctcatga tgatcgcatc ttcatagttg tacccttccc aagtcatgaa accaactaaa
    25681 acgttttggc ctagagccat ttcgccttgt tccatagatg gtccgtctgc cagagtatcc
    25741 cctttttcga ctttctcacc caaatgaacg attggacgtt ggttgtagct tgttccggag
    25801 tttgaacgac ggaattttgt tacatcgtat ttatctaatg caccgttgtc gcggcgtacg
    25861 cggatttcag aagcatcaac gtattcaacg acaccgtcat gtttacataa caaagcagct
    25921 cctgagtcat gcgctgattt gtattccata ccagtaccaa cccatggtgc ttgaggattg
    25981 atcaacggca ctgcttgacg ctgcatgttg gcacccatca aggcacggtt ggagtcatcg
    26041 ttttccaaga aaggaataca tgctgtcgca acggcaacta cttgtttagg tgatacgtcc
    26101 atgtagtcta ctttatcaat agatacttct aagttttcac tttgagcacg agccataaca
    26161 acgtcttgag caaatgttcc atcttcattc aataggctgt ttgcttgggc tacgatgtaa
    26221 tggtcttcga tatcagctgt caagtagtcg atttggtcag taacacgacc tgtttcacgg
    26281 tctacacgac gataaggtgt ttcgataaat ccgaatttat ttactttcgc ataactggac
    26341 aaactattga tcaacccgat gtttgggcct tcaggagttt cgatcggaca catacggcca
    26401 tagtgggagt agtgaacgtc acgaacttca taaccggcgc ggtcacgagt caatccgcca
    26461 ggtcctaagg ctgaaagacg acgtttgtgt gtcaattcgc caagagggtt tgtttgatcc
    26521 atgaactgtg acaactgaga agaaccaaag aattctttga tacttgccac aactggacgg
    26581 atattgatca attgttgagg tgtcaatgtt tcagtatctt ggatcgacat tctttcacga
    26641 accacacgtt ccatacgtgc tagcccgata cggaattggt tttgtaaaag ttcaccgact
    26701 gaacggatac gacgatttcc taagtggtcg atatcatcga cattgccgat accttccatc
    26761 aagttcaaga aatagctcat tgaagcaatg atgtcggctg gacgaaccgt ttttacagct
    26821 gcttctggat agccgtttcc gatcacgttt acttcacgtt cagggtctct tggtgaaaat
    26881 actttgatga cttggacagt cattggatct gtgaccacac cgtcttctga tggatagtaa
    26941 gtcactgaat ttaggccatt atcaatgaaa ggagctagtg tttccatgac ttggtgagtc
    27001 agaactgttc ctttttcaac gatgatctcg cctgtttctg gatcaactaa tgtttcagct
    27061 aatgtcagat tcaatagacg tgttttcaaa tctaattttt tgtttacttt gtaacgacca
    27121 acgtttgcta agtcgtaacg ttttggatcg aagaaacgag cattcagcaa gttacgtgag
    27181 ctgtctgctg tttttggttc gcctggacgc aaacgttcgt aaacgtcttt caaaccttct
    27241 tctgttcttg agtcgcttgc gtttttatgc aagtcttttt cgactgtatt gcgtaatgtt
    27301 tcgctgtcgc cgaagatttc aaagatcgta tcatcggaac caaatcctaa cgcacgaact
    27361 aatacggtta aaggaatctt acgtgtacgg tcgatacgta cataagagat gtcttttgca
    27421 tcagtttcca tttctagcca agctccacgg ttagggataa cagtagaacc gaatccttct
    27481 tttccgtttt tgtcgacttt tccatggaaa tagacacctg gtgaacggac taactgagaa
    27541 acgattactc gctctgcccc gttgatgatg aatgttcctt gttctgtcat taatgggaaa
    27601 tcaccgaaga atacttcttg tgctttgatt tcgccagtct cgcggtttgt caaacgcaat
    27661 gttacatgta atggtgcaga gtagtttgca tcatgcgcac gagcttctgc tactgtatac
    27721 tttggttctt tcaattcata atctacaaat tccaaagata gattgccatt gaagtcatcg
    27781 attggcaaaa tgtcttcaaa catttctcta agtccctcat ctaagaacca ttggtaagag
    27841 tctgtttgaa tttcgatcaa atttggtaat tccaacactt cactgattct tgcgaaactt
    27901 ctacgttcgc gatgttttcc gtattttact acgtgtccag ccaagctctt cacccctcta
    27961 aaatgtcatt caaaaagact acccgatcaa ttgttacaat aatatttcaa atattatttt
    28021 taggtaacaa cagacatttt ttgcggcttt taggcaaaaa aaaaccaaaa atagaacatt
    28081 ctcttgaaac cttacttcgt gagcttctac ttttgtttcc ttatattaaa gccgaaacgg
    28141 acaatatttc gtttctgcct tttcttcatc agatagtttt tgcaacttca tatactacac
    28201 ttattttcta taaaagtcaa cacttcctgg cgtttcttct gctttttcgc aacattttct
    28261 taatgttttc aattttatgt tttcaattat ctgacaattc ttaattgaca gaccttttta
    28321 tctcggttat actttagaaa attcattttg tggaggaact tctatgcgaa ccaacatcaa
    28381 ccaaatcgca ccaacacaac tgaataactt ttactttagc tactttagat aggtttgtat
    28441 tcatcatgat ggatacaaac gttaaacgac agtttgtatc tatgatggct aaagaacttt
    28501 gtattgcagc aaaaagccac gtagacaaaa tgctaagtgg ctgagcagtg aaaatacaaa
    28561 gtaaaagtat caagcattct actttttcaa tttattttcc gacttttcaa tccatttgga
    28621 cattttgtct gaatggattt tttgtttccc aaaattaagg aggaaaaaga aatgaaaaaa
    28681 aatataatca tcggattagg cgctgccctg ctactagctt taggaggttg cgcacaaaaa
    28741 gacaattccg cagaggcatc agatggacaa aaaacaattg gcgtattgca acttgtacaa
    28801 cataactcgt tagatgctgc ctataaagga ttcaaagaag gattagcaga agaagggtat
    28861 aaagaaggcg ataatctaac aatcgattat caaaatgccc aaaacaatca agacaacctg
    28921 aaaagtatga gtgagaaact cgtcaaagaa gatcctgatt tgctacttgg aatcgccact
    28981 ccagcagcac aatctttatt aaatgaaaca accactatcc cgatgactgt cacagcggtc
    29041 acagacttgg aacaagccca tttagtcaca tccaatgaaa aacctggcgg caatgtgact
    29101 ggcacgacag atatggtgcc aatcgaaaaa caaatcggct tactgttaaa tatcgtgcca
    29161 gacgccaaaa caatcggcat catgtataac aacggtgaag caaactctaa gatccaagct
    29221 gatttggcag aaaaagaatt gaaaaaagcg ggcgtcaaag tcaaagtact gacagcaaat
    29281 tccacaaatg atgtccaaca agtgacgaaa agtctagcta aagatgtaga tggaatctat
    29341 attcctactg ataacacgtt cgcaagtgcg gcaagtgttg tcggtgaagt cgcaaaagaa
    29401 acaaaaacac ctgtcgttgc tggcgcagtt gatcaagtca aagacggcgg tctagcaaca
    29461 gtaggaatcg actacgaaag cttgggcaaa caaacaggga tcatggcagc aaaaatctta
    29521 gacggcaaga atccaagtga acttcctgtc gaaacagcga atgatttgga actgtatgtc
    29581 aataaagaca tggccaaagc attaggcatc aatccagaca gcatcaaaga agctgaataa
    29641 aacacaggag gattctcatg acaaatttgc tctctctttt cttatcatct gcatcgcaag
    29701 gggtcttatg ggccctactt gcgattggtg tatacgtcac tttccgtatc ttggacattg
    29761 ccgacttgac tgctgaagga agctttcctt taggtgcagg gatcgcggca gtaagtatta
    29821 caaatggcta tcatccattg acagcttgtc ttctaggctt tttagggggt gcggcagcag
    29881 ggcttgtttc cggactgctt cacacaaaat tgaaaattcc cgcccttctt gcagggatca
    29941 tcaccatgac tggtttatat tctgtcacat cccgtgtgat gggtgcgccg aacatctctt
    30001 tattgggtca gccaacgatc tttcattggg tcgagtcatt cggcataaca aaagacaacg
    30061 ctgtactgat cgtcggtctg ctttttgctt tgatcgttgt atgtttactt gttttgttct
    30121 tacgtacaga aacaggactt gctattcgtg caactggcga taatctggca atgagcgaag
    30181 caaacgggat caacacagat aatatgaaga tcatcggtta tatgatttct aatggatgca
    30241 ttgctctttc tggctcctta cttgcgcaaa acaacggctt tgctgattta aattctggta
    30301 ttggaacgat cgtaattgga cttgcttcta ttatcatcgc agaagttttg ttacgtaacc
    30361 agccactatt tctccgtctc ttgactgtcg ttttaggtgc ggtcatttat cgttttattt
    30421 tagcgttagt cttcgaactg aatgttgaac caagtgattc gaaacttgct tctgctttcg
    30481 tgctagtgat ctgtctttct ttcccgcaga tccagcaaaa attccattta cctcaaaaaa
    30541 agaaaggcgg tagtgtcaaa tgaatgacgt cttaacattg gaaaatctcc atcatacctt
    30601 tgaaaaagga acgatcaacg aaaatcatgt cctgaaaggc attaatcttt ccttagatcc
    30661 cggcgacttc gtgacgatca tcggtggaaa tggggctggt aaatctactt tgctgaacag
    30721 catcgccggt acattttctg tcgatcaggg tcaaatttta ttgaatggaa aaaatatcac
    30781 gaaaaaatct gttgtcgaac gttcgaaatc catcagtcgc gtattccaag atcctaaact
    30841 agggacagcc gttcgtttaa cggtagaaga aaatcttgct cttgctatga aacgaggaaa
    30901 aaaacgcggc ttcttccgtg gtgtcaaacc acaagaccga acatttttca aagaccagct
    30961 ggttcggcta aatttggggt tggaaaatcg tttatcctca gaaatcggtt tgctgtctgg
    31021 aggacaacgt caagctatta cgttattgat ggccacatta cagcgtccag aactgatctt
    31081 attagatgaa cacactgcag cgcttgaccc tgctacttcc aaaacggtga tggaattgac
    31141 agaaaagctg atcactgagc agaatctcac tgcttttatg gtcactcatg acatggaaga
    31201 cgcgatccgc tatgggagtc gactgatcat gctgcatcaa ggaaggatcg tcgtagatat
    31261 ccagcaagag gaaaaacagc agctcacagt gccccaatta ttagcgttgt tcaaacaaaa
    31321 cagcggcacg gcattaaaag acgatcaagt acttttatct tcataatcag aaagaggatg
    31381 ggacaaaaaa tgaacgccca ccctcttctt ttttctcttt ccttctttcg tttttgcttt
    31441 atgtatcaac cgttgtttct tcgaaaacta ttttgttctc tccttcttcc tgatagaatg
    31501 gatgggaaag gagcttgatg ccatgacaac gaaagacaag gtacttcaat tattgaaaaa
    31561 gacaccagat tttctttccg gagaaaagtt ggcacagcaa ctggaagttt cccggacaag
    31621 tatttggaaa gccatcaaag aattggaaaa ggcaggctat cgttttgagc acgggcctac
    31681 agggtatcgt tacctgccct ctgacgtact agatgcaaaa gaaatccagc aagagctacg
    31741 gtcgtttatt cctgatttga aagtacaaac gatgttgact tctgaatcta cgatgaaaga
    31801 tgccaaactg gcagcagctc aaggagattc tgtttcgaag ttatttgttg ctgatacaca
    31861 agaacaagct cgtggacgtt ttggacggcc ttttttcgct gaacctggac gaggaatcta
    31921 catgagtctc ttgctccatc cagacaaacg gtttgatgaa ttgcctcaat atacgatcat
    31981 cgcagccagt gcctgtgtga aagccattga atctgtaacc ggtcgttctg cttcgatcaa
    32041 atgggtcaat gatatctaca tggatggaaa aaaagtctgt gggattttat ctgaagcagt
    32101 cagtgatatg gaaagcggca ggatcagtca tatcatcatt gggatcgggc ttaatttttc
    32161 tgttcctcaa aatcattttc cagctgatct ccaagaaaaa gccatctctg tctttcccga
    32221 cggacaagct agtgtcacaa ggaatcagct aatcagcaaa atctggcaag aattgttcac
    32281 ttatctttct cacccagaaa aagcgttgga cttctataaa gaaaaatcat tcgtattggg
    32341 aaaaaccgtg tcgttcaatc aaaaaggaac gacttatgtc ggtcttgcaa aagaaatcgg
    32401 tgatcatggc gaactgatcg tccaaacaaa tgaacagaag atgagtttgt cttctggcga
    32461 aatcagtctc acatctatcc gtacgcaata attatcagat ttcacaaaag gacacctgat
    32521 ctcatacagt gtcctttttt ctgaattctc ttcaaaagac tctccttatt ctatcagctg
    32581 acagtttctc tatcctctag aaaaaggtat tctaaaaata tctataaagc atggtacact
    32641 ttctttaaat gaatcaatgc aaaaaacaag agagtcatca gtttacacga tcaaaagaga
    32701 tagcaggccg cttccttttt caatcttata gatagaccat gggtaacgat aaaaacaaaa
    32761 aagaacttgc gacttatttt gccgtaagtt cttttttagc tagtgcctta atctcttaat
    32821 cccaacaaac gtctcgcata gatcacctga ccagagtgca tagcagcatc atcaataata
    32881 gaaacgagac gcattcctcg tgtgactggt ggtgtccagt tctcgtcaat tacttcatct
    32941 agctcggaag gttccagcct tcttaagtaa gaaatagcaa aatcagcagc tcctcgcaag
    33001 tatcctagca gatcttctat ttgattagct tggatctgct ttgcttgttc cagactatgc
    33061 ttccaatctt gttcgttatc tgctatcgtc atcggaaacc ggctttccca gtcttgacta
    33121 tgccaaattg gagaagttcc tgctaaatca gcaatctgga aatctaattc tcgagcggta
    33181 tgccaagcca gccaagtcat tgatttgatc tgcggggctc tatcttccac tggaaaacga
    33241 ttggcctcct caatagagac gccttcaaac aaacgttcaa accgatcgta tgcccgatcc
    33301 acactctcgc atagcatatc taaataatcc atttattttc ctccttttta tttatcctac
    33361 ttactttttc ctaattctag gatacctact tatatgttac tacacaccct tattttttac
    33421 actttaacag tcaccatcat caatagtttg gagattgaaa gaaaaagata gtgtcgaact
    33481 atcaattgaa caagaagcaa caatatgaac gtttcattaa tattctttaa ataaacaatg
    33541 atttttcaaa cgaacagttt aagactagga gaaaaagagt ataataggaa aatgtttgaa
    33601 aggaaaggaa ggggaaaaat gaaaaaaagg gcaagtttac tagtcctagt tgctttgttg
    33661 ttcaatatct tttcgtatgg tgtggccgtt tcagcggaaa catacattcc taaagaccct
    33721 gggaatattt ataatgatct aactggtaac tatggcttgt taggaattgc tagtcaattc
    33781 cacgtatttg ctaaaaataa gacaactgtg aatgcacata cagacggaaa tatagcaaca
    33841 catgaacttg atgcaaaaaa taattttggt acagagcttc atgaaggttt attgaaccaa
    33901 gaaataaatt atatacaaaa aactgattct ttcgctaatt cttcaggcat acctacaggt
    33961 agcacaagaa tgaataaatt tgtcgttggc agtaacgtgg atgtagcact tcaagatggt
    34021 catcctacag tcaatggatc caggcttgat cacttaactc cagatgattt ttataaagat
    34081 cgtgctgggc atgaatatat tgatttcgag caggaatttg ctaaattgaa tgtagcagca
    34141 aatgatcttg caacgatcac tccagctaaa acatatactg ctgcagattt tccagacatg
    34201 aacaatcgga caatcgattt aactgattta tcagataccg ggtttttatt ggtaaatatc
    34261 gatgcagaag tgttaacgat gaatacgcca cttcaaatca ttaatccaaa tgatcaagta
    34321 gttgtattta atgtcatcaa cagttcttct gcattgaacg tacagtcacc aatcaaatac
    34381 aatgatcgtt caaatcatga aacagaagat ttttcggatg cgaatatctc ttggaatttt
    34441 ggaaatgaga tgactgattt gacaatcagt gctccttttc aaggaacaat ccttgcgcct
    34501 aatgctacta tccgcattac tcaaaaccaa gacggaacga ttataggaac aaatgtgatt
    34561 ttagatgcag ctaccaatcg ttgggatcct aacgaaatat ttatcagcga caatggtaca
    34621 gatacgacag acacaagcga cagtacagat acatctacca caagtgatag ctcagattca
    34681 tcaacttcaa gcgacagtac agatactacc tctaccacaa gtgatagcac ggatacatca
    34741 gcttcaagcg acagtacaga tactacctct accacaagtg atagcacgga cacatcagct
    34801 tcaagtgaca gtacagatac tacctctacc acaagtgaca gcacggacac gtcagcttca
    34861 agcgacagta cagatactac ctctaccaca agtgatagca cggacacatc agcttcaagt
    34921 gacagtacag atactacctc taccacaagt gatagcacgg acacgtcagc ttcaagcgac
    34981 agtacggata catctaccac aacaagtgat agcacggaca cgtcagcttc aagtgacagt
    35041 acagatacta cctctaccac aagtgatagc tcagattcat ctaccactac aagtgatagc
    35101 acggacacgt cagcttcaag tgacagtaca gatacatcct ctaccacaag tgatagctca
    35161 gattcatcaa cttcaagcga tagtacagac acgtcgtcta ccacaagcga tacacgtcat
    35221 gcaagtgtct ccttctcaaa aacaagttcg gataacagga cgaactttaa cttaactaat
    35281 aaaacaagca atgacaatgg aaacaaatca aaaagagcac ttcctaaaac tggaagtcaa
    35341 tcgaataatt ggataacact cgcaggcgtt atattacttg taatcggttt aagaatcact
    35401 tttatgagtt ataagaaatc gaaaaggtaa aaaaatattc aagataaaaa agagcaacgt
    35461 cgctaacgga cgttgctctt tatttttgct ttttatcata aatacaagaa ctacaattca
    35521 atttgtcaaa aaacacgtag atttccttct gagaaattta tttacgtatc gaacctttcc
    35581 ctattcagtc tttgcgatcc aaaatcagtt gggtaaggga aaagagattc tcttttgttc
    35641 gaagagaggt ggcaggtaat ttctttattt ccttcaaagc cttttctgtg taagaggccg
    35701 ctaacttcct agcttcttcc acgcctcctg tatgttcaac gattttgcgt acggcgtcga
    35761 catcttcttc tgtcattgcc gctttcttct ctaaataagg tagtagcttt ggtttgtttt
    35821 tttgcaaaga gtagatcaac ggtaaagaat agaccccttg acgcatgtct tctagtacag
    35881 gtttgccgat ttcctggctc tcttttgtat aatctaaaat atcatcgata atctgaaagg
    35941 ccatcccgat agaaagtccg atatctcctg ctttttttgc gaagcgctcg cttgttccac
    36001 tttcaaaagc gccaacagaa caacttaatt ggaacagttc agctgtctta cctgaaatat
    36061 tttccaaata ttcttttaca gaaagttcat aattatagcg atcatccatc tgccctaatt
    36121 cgccattcaa tactttttcc atactgcgag aattcaattg gagacttttc aaggaagtgg
    36181 agtaatcaga caatagttta aaacagcaga caaataaata gtctcctgca tagacagaaa
    36241 cgacattacc aaactgacta cggatattag gctgaccacg tcgcagatcc gcttcatcaa
    36301 cgatatcatc atggatcagt gtggccgtat ggagcatttc gatagaagct gccaaagcga
    36361 tggctttgtc tcgatcttgc tctggtccaa attgtgagaa caatagctga taggcaggac
    36421 gcaacagctt tccgccggca tggatcgttt ctttgatggc ttgttcgaca ggtttgtttt
    36481 tcaggcggac agtttcttcc atccgtttta gtgtttctgt caattcttta cttaaagcag
    36541 gatagttttc ccacattgga tgtagcttca taaaaaaact ccttgtgtga cgattatttt
    36601 ttggtaaaca ttgggctcca tttttgtttg gttccctgct cataatcttt ttggaatcgt
    36661 tgaagtgtct gcacatgtcg taaaagataa ttcgctgcga tcccgattgc aatccctgat
    36721 aaaatcccta aaactgaaag gactggcaag tacaacatga ctgtccatga ttgtgcaatg
    36781 aaagaagcca tcaaaagctg tccgacatta tgcatgaaac cgccggccgc actaattccg
    36841 atgatactga cacgttttgg tcctaattgc ttgaccaata acattccaaa ataactaagc
    36901 aaagcaccac tcatactata aaggaatgtg gaaatcgttc cgcctagcaa agtagtcagg
    36961 aatagtcgta gccaaagtag taagaagctg tcttgttttt tcatggtaaa aatggcaacg
    37021 atcgtgatta gattggctaa gcctagtttt gcaccaggcg caaaagaaaa aggataaggg
    37081 atcatatttt ccaataaacc aatgatcact ccttgtgcaa caagtagcga aatataaatc
    37141 attttttgta atttactcat ggtgtgtgaa ggtctaataa atcagactgt cttcgtcacc
    37201 tccatctgtt gaacggactt cgatcactaa tttatgtggc aaacagacga tggtttcgcc
    37261 atttttcgtt gcccagcctc gacgtacgca gatctggtca tcgcaatctg cttcttttat
    37321 ccgtactcga tcgccatcta tctcgatcaa gttataatct ccgtgttcat ctttgtaagt
    37381 atatgtgttg ctctcttctc cagccatcaa agggaatgtt ttgatttctg tgccatccac
    37441 acgcagtacc gcttcttttt ctgctgttgc ggtgttttgc tgaagaccaa aaatcatgac
    37501 tggtaaaaaa gaaagaacag tcaaaagcaa aataataaaa acatcccaag gtttgagcag
    37561 acttttttca gcaaattctt taaggctcat ttttgttctc ctttttccag attaactcac
    37621 ccaattttag cataaaaaca agaggttgtg acacctgtgt cttgctttga gtaataagaa
    37681 cgaagaatag aaaacagctt tccatgtttt ctaattcttt gggcttatta ccgagaagca
    37741 gcaggtggaa caccgtttat aacaagaggt tgtgacagaa gtgggcaact tcaagaaata
    37801 agaaggaatt tacgaaaatt acttttcaag tttttgtgaa atttcggctt atttttcgaa
    37861 gaagctactt ctggaacacc gtttataaca agaggttgta acagaggttg tgacacctgt
    37921 gtcttgctca tggaggctta tctcgggcat cttattcctt taattttaca taaaaaggag
    37981 gctgtgacgg tttgtctcag cccctttcta ttgaaatctt gcattcaaat aacgttcttt
    38041 atctatttat ttcttgccgt actgggaact tggtctaccg taccaccaat tacctagtgt
    38101 acgttgtagc catggaatta cccagcgatc caacccaatc gcacgtccgg aaccgttcat
    38161 caatgcgaaa gcaacaaaaa tgaaccaaat gtttacccag taaaacatgc ctgataagca
    38221 gaacgtgatc accaatacaa tcgttgtcgc actgcatagc catgtgaata atccagcaat
    38281 caatgctaat gccaaggcaa tttcaaagaa taccatgaat ttttggaaga acattgctac
    38341 atcattgttt ggcatgatca cttgcatgat ctttgtgaac cagtctggtg acttctcaat
    38401 cacagccatt ggttcttcac cgtatgcata actcaaacca aaatgtgcag cttgagtcgc
    38461 tgcttctccg gcacttgccc ctgctccgct ggcagctgtg gttgcatcac cagcagcaga
    38521 agcaccactg gcaacatctg ttacattgta ttcccatgtc caagggaaca cactgtttcc
    38581 agtgaaccat gatccttcac caaaccaagt acttggtttg aacacttcac cattcccaat
    38641 gatttttttg gaagcttcaa cgaaccaaac taaaccatag aaaatacgaa gtggcacact
    38701 ccataagaca ttgccgtaac gagaagaatg tcctcgagtc acgttgcgat catctttgat
    38761 atggaaaaac tcatgcatta aatattggaa catataataa ccagaacgaa tatcaaagaa
    38821 gtatttcaaa ttcacgatat gtttcattac gatagctaaa aatccgctta aatggatctt
    38881 atcaaatagg ttagctaccc cccatttggc accaatagaa accataaatc cttgatagtt
    38941 tcctttaaat ttatgctttt ctccgccttt gatcgaagcc acgatattcg ctgcggctgt
    39001 gtgtcctgtt tgttcagcag cttgaacgat ctgcggtgtc ggtgttcctg ggaattcttc
    39061 ataaaagact aaatcaccaa tgatatagat gtttttatct tcgtagcctt ttgcctgcat
    39121 atattcattg gcgatcaacc gctgtccgcg tgctgattct aatccaaagt cagcagcgtc
    39181 agaagttcct ttgacaccag ctgtccaaat cagtgtgtga gtcggaatgt tgcgtccgtc
    39241 tttcaatttg atatgatcag cagccacttc tacgattggt gcattcaata ataattccac
    39301 gttgtgctta cgcaaatagc gttcagcttt tgcagcatca tttcgatcca acatattcaa
    39361 gatcgtcggc attgcctcta cgaccatcag tgtgatttcg tttggatcta atttataatc
    39421 tttggctagg cgatttttcc agtcgatcag ttccccaatc atttcaatac cggtaaatcc
    39481 agaaccacag acaacgaaag tcagcatggc tttacgcagt tcatcatctg gttccaatgc
    39541 agcttttgct actgtcttct caatatgttc ccgaatcctt acagaatctt caaatgacca
    39601 aagggtgaat ccgtgtgctt tgacaccagg tgtaccaaag tcattcggct ctccgcccat
    39661 tccaaggatc agctgatcaa aaggatagga acctaatttg gtcttgacga tttttcgttc
    39721 tttatcgata ccgattacag tgtctgtgac aagttgtaca ttcttttgac gagcaaacaa
    39781 atgctgcaga tcgtattgga ttgcttctgg ttctacacgt ccgccagcta cttcatgcaa
    39841 ctcggtcatc atagtatggt acgaatgacg atcgatcaga gtaattgcta catcttcttt
    39901 tttcagtttc tttgccaagt attttgtagc tgaaacaccg gcatatcctg ctcctacaac
    39961 gacaatattt tgctttgcca ttgatcatcc gctccttata gactatctat ataatgaata
    40021 tatgataatt cttttagttt gatacttatt acacaatctc catatattat atagatgttc
    40081 tgttcttttg tc
//
LOCUS       DAZUMV010000012        39842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000012 DAZUMV010000000
VERSION     DAZUMV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.012"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..907
                     /locus_tag="ACG8FK_000560"
     CDS             38..907
                     /locus_tag="ACG8FK_000560"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS5096197.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1072..2343
                     /locus_tag="ACG8FK_000561"
     CDS             1072..2343
                     /locus_tag="ACG8FK_000561"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5096198.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2340..3626
                     /gene="rho"
                     /locus_tag="ACG8FK_000562"
     CDS             2340..3626
                     /gene="rho"
                     /locus_tag="ACG8FK_000562"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS5096199.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKIRPEI"
     gene            3733..3996
                     /locus_tag="ACG8FK_000563"
     CDS             3733..3996
                     /locus_tag="ACG8FK_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS5096200.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4441..4890
                     /locus_tag="ACG8FK_000564"
     CDS             4441..4890
                     /locus_tag="ACG8FK_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5096201.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4947..6365
                     /locus_tag="ACG8FK_000565"
     CDS             4947..6365
                     /locus_tag="ACG8FK_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5096202.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6365..7225
                     /locus_tag="ACG8FK_000566"
     CDS             6365..7225
                     /locus_tag="ACG8FK_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5096203.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7399..8172
                     /locus_tag="ACG8FK_000567"
     CDS             7399..8172
                     /locus_tag="ACG8FK_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5096204.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8319..8684
                     /locus_tag="ACG8FK_000568"
     CDS             8319..8684
                     /locus_tag="ACG8FK_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS5096205.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8832..10040)
                     /locus_tag="ACG8FK_000569"
     CDS             complement(8832..10040)
                     /locus_tag="ACG8FK_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5096206.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10059..10337)
                     /locus_tag="ACG8FK_000570"
     CDS             complement(10059..10337)
                     /locus_tag="ACG8FK_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096207.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10530..11186
                     /locus_tag="ACG8FK_000571"
     CDS             10530..11186
                     /locus_tag="ACG8FK_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5096208.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11628..12425)
                     /locus_tag="ACG8FK_000572"
     CDS             complement(11628..12425)
                     /locus_tag="ACG8FK_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS5096209.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12422..13606)
                     /locus_tag="ACG8FK_000573"
     CDS             complement(12422..13606)
                     /locus_tag="ACG8FK_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS5096210.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13631..14878)
                     /locus_tag="ACG8FK_000574"
     CDS             complement(13631..14878)
                     /locus_tag="ACG8FK_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5096211.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15093..16838
                     /gene="dnaX"
                     /locus_tag="ACG8FK_000575"
     CDS             15093..16838
                     /gene="dnaX"
                     /locus_tag="ACG8FK_000575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS5096212.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16858..17172
                     /locus_tag="ACG8FK_000576"
     CDS             16858..17172
                     /locus_tag="ACG8FK_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS5096213.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17197..17793
                     /gene="recR"
                     /locus_tag="ACG8FK_000577"
     CDS             17197..17793
                     /gene="recR"
                     /locus_tag="ACG8FK_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS5096214.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17857..18501
                     /gene="tmk"
                     /locus_tag="ACG8FK_000578"
     CDS             17857..18501
                     /gene="tmk"
                     /locus_tag="ACG8FK_000578"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS5096215.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18516..18845
                     /locus_tag="ACG8FK_000579"
     CDS             18516..18845
                     /locus_tag="ACG8FK_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS5096216.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18859..19797
                     /gene="holB"
                     /locus_tag="ACG8FK_000580"
     CDS             18859..19797
                     /gene="holB"
                     /locus_tag="ACG8FK_000580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS5096217.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19833..20657
                     /locus_tag="ACG8FK_000581"
     CDS             19833..20657
                     /locus_tag="ACG8FK_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS5096218.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20650..20997
                     /locus_tag="ACG8FK_000582"
     CDS             20650..20997
                     /locus_tag="ACG8FK_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS5096219.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21066..21938
                     /gene="rsmI"
                     /locus_tag="ACG8FK_000583"
     CDS             21066..21938
                     /gene="rsmI"
                     /locus_tag="ACG8FK_000583"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS5096220.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22047..23168
                     /gene="dinB"
                     /locus_tag="ACG8FK_000584"
     CDS             22047..23168
                     /gene="dinB"
                     /locus_tag="ACG8FK_000584"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS5096221.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23222..23824)
                     /gene="nrdG"
                     /locus_tag="ACG8FK_000585"
     CDS             complement(23222..23824)
                     /gene="nrdG"
                     /locus_tag="ACG8FK_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS5096222.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24139..26328)
                     /gene="nrdD"
                     /locus_tag="ACG8FK_000586"
     CDS             complement(24139..26328)
                     /gene="nrdD"
                     /locus_tag="ACG8FK_000586"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS5096223.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26654..27421
                     /locus_tag="ACG8FK_000587"
     CDS             26654..27421
                     /locus_tag="ACG8FK_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096224.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27411..29453
                     /locus_tag="ACG8FK_000588"
     CDS             27411..29453
                     /locus_tag="ACG8FK_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096225.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            29734..29883
                     /locus_tag="ACG8FK_000589"
     CDS             29734..29883
                     /locus_tag="ACG8FK_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS5096226.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            29894..31408
                     /gene="dltA"
                     /locus_tag="ACG8FK_000590"
     CDS             29894..31408
                     /gene="dltA"
                     /locus_tag="ACG8FK_000590"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS5096227.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            31405..32610
                     /gene="dltB"
                     /locus_tag="ACG8FK_000591"
     CDS             31405..32610
                     /gene="dltB"
                     /locus_tag="ACG8FK_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS5096228.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            32655..32888
                     /gene="dltC"
                     /locus_tag="ACG8FK_000592"
     CDS             32655..32888
                     /gene="dltC"
                     /locus_tag="ACG8FK_000592"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS5096229.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            32891..34159
                     /gene="dltD"
                     /locus_tag="ACG8FK_000593"
     CDS             32891..34159
                     /gene="dltD"
                     /locus_tag="ACG8FK_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS5096230.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            34300..35382
                     /locus_tag="ACG8FK_000594"
     CDS             34300..35382
                     /locus_tag="ACG8FK_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS5096231.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            35382..36185
                     /locus_tag="ACG8FK_000595"
     CDS             35382..36185
                     /locus_tag="ACG8FK_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5096232.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            36255..36995
                     /locus_tag="ACG8FK_000596"
     CDS             36255..36995
                     /locus_tag="ACG8FK_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5096233.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            37006..38010
                     /locus_tag="ACG8FK_000597"
     CDS             37006..38010
                     /locus_tag="ACG8FK_000597"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS5096234.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(38055..>39842)
                     /locus_tag="ACG8FK_000598"
     CDS             complement(38055..>39842)
                     /locus_tag="ACG8FK_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096235.1"
                     /translation="WSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKD
                     DPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQ
                     RKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFI
                     YSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDI
                     LSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLY
                     ATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLI
                     YIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQ
                     HYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFC
                     NHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAI
                     ENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVAD
                     RYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        12777 a         7018 c         8268 g        11779 t
ORIGIN      
        1 caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat caggagctga
       61 atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca caaacaactt
      121 ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag tacttatcca
      181 aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag atttaatcac
      241 tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag accatggtga
      301 ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt tcgatggttc
      361 acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa aagcacatgc
      421 aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag acggaatcat
      481 cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta caggtatcga
      541 ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact ggaaaggatt
      601 agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc ctttagtatt
      661 acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa tgggtatctc
      721 aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc gtaaatatat
      781 cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat tagctccagg
      841 taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt cagctaacaa
      901 agcgtaagca tccacataac agatacgata tataggaatg gagaacgact tttgaagtcg
      961 ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt ttatctatga
     1021 taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca gatgaaaaaa
     1081 atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg tgcaaaaaat
     1141 agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat cttggaaggt
     1201 gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat gggcgcaact
     1261 gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc gattccgatg
     1321 ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttatgggaac actgcttggt
     1381 aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc tagaccgatt
     1441 gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga acatggtgcc
     1501 atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat ggacgtggtt
     1561 tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg acagacagta
     1621 attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact gaataatatg
     1681 ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt cgaaacatta
     1741 catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta tctagcatta
     1801 gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca tttggaaagc
     1861 tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga tgaaatcgaa
     1921 gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta tccaggattt
     1981 gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg cacttcagaa
     2041 atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc acgaatgggc
     2101 gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa acttcacggc
     2161 acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc tggactattg
     2221 gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta tgatcatatt
     2281 atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt gggagctgaa
     2341 tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa gtttatagtt
     2401 atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa ttatctctag
     2461 ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga atattggata
     2521 tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca agtgcagaag
     2581 atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg gacaaagtgg
     2641 caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg cacgtggaaa
     2701 gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca gccttaacac
     2761 ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta tctacccgaa
     2821 tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc gcaccgccaa
     2881 aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa aactatccgg
     2941 aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg gatctagaac
     3001 gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa aaccatactc
     3061 gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa cgagatgtcg
     3121 ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc gttccaccaa
     3181 gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct aagaaatttt
     3241 tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg accgctttag
     3301 tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga actggaaatc
     3361 tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc atcgatatca
     3421 aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta gaagaaattt
     3481 ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa ctgatccgct
     3541 ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat gtctcctttg
     3601 gtaaaaaaat ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa
     3661 gatgttaatg ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg
     3721 agcgaaaaaa tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat
     3781 tcaacaactg gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa
     3841 tgggaagacg gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca
     3901 ttctacactg gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa
     3961 aaatacggtc tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt
     4021 tagcagacta tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc
     4081 gtttgatgct gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata
     4141 gttttaatta tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc
     4201 gaagtaaaaa accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt
     4261 tgttttgtta tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac
     4321 acgcggatga tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga
     4381 tgctatactg acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt
     4441 atgaggtttg atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca
     4501 aaaggatcat tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat
     4561 gttcaaaaag tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa
     4621 gatggatttg ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt
     4681 tttcatacag ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt
     4741 tttgccatcg tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc
     4801 tccagacgat tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca
     4861 gatatcatca gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat
     4921 agtgaagaaa aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg
     4981 gaattgctca tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc
     5041 atcaagcaaa attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa
     5101 tcaaagaggc agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg
     5161 ccgggaaaaa agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa
     5221 tagacgaagc attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg
     5281 caaatgagaa aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc
     5341 catttgtgat cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg
     5401 ctgatcccaa ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag
     5461 atacagcgtt tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg
     5521 gaaaagcagg ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag
     5581 gttttcttgg cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa
     5641 ataaaattag cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt
     5701 tatcgatctt aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg
     5761 tgttggatgc tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg
     5821 gattacttat cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt
     5881 cgactttctc gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg
     5941 ctgcaggaat gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat
     6001 ttaccaaaga agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta
     6061 cagaaggtgc tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga
     6121 caggttcagc agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc
     6181 atggagggat ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct
     6241 tggcaattgc atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag
     6301 tgaaagagaa aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag
     6361 cgtaatgtta tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt
     6421 accagccttc aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag
     6481 attagaagca ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga
     6541 gtttgttgcg gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt
     6601 ggatcatggc ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt
     6661 gatgatcgat gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt
     6721 tgcgttagct catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa
     6781 tggttctgct gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat
     6841 ttttgtgaaa gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt
     6901 gtatccgaaa aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca
     6961 aatcgatctt ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga
     7021 agcagtaaaa tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt
     7081 caatgctttg agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta
     7141 tccagcagct aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac
     7201 agtaggcaag gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata
     7261 gtcttgtcat accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc
     7321 ttcttatttc tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa
     7381 agtaaatggg gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat
     7441 tatctaaaaa acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc
     7501 gaagccacga ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt
     7561 cgcggtggcg ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc
     7621 accaaattag aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt
     7681 cgagatgata tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac
     7741 ctagcaagat ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg
     7801 agtgaatata caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca
     7861 acaatcaacg gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata
     7921 atgagttgtc gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca
     7981 atggtcaaaa aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa
     8041 aaaattgcac ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg
     8101 acaagtaaag aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt
     8161 ctgtacggat aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc
     8221 ttatctttgc tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat
     8281 aatcttactt ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg
     8341 tagtgaaggc aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg
     8401 ttagctttat ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag
     8461 gggatctgtt gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt
     8521 cagctgcagt tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga
     8581 tgaaagtatt gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt
     8641 tgctaagtca aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta
     8701 ttcttatcga taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa
     8761 actattttta ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct
     8821 attcatcaag atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga
     8881 ctatgttttc ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca
     8941 atcacataca atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca
     9001 acgcctaagc ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt
     9061 tcgcggacga tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg
     9121 ccgactaatg ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat
     9181 aatcctgctg ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg
     9241 aaaatccttc cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa
     9301 aatgacccag cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca
     9361 cttgaataag aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca
     9421 ataaaactat tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata
     9481 ttttttacag gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac
     9541 cagataatga aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca
     9601 gcggcagcta aactagtact taatccataa taattgatgc cttccccttt tttagatgca
     9661 gggatatatg cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca
     9721 ttcaagaatc gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt
     9781 gaaaggagat agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac
     9841 tgtttcccca tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa
     9901 cctgcttggc tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc
     9961 aacagataat aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta
    10021 ttaaataatt tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat
    10081 ttttgtattt gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga
    10141 ataaataaat ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga
    10201 ttctcatcca ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact
    10261 aaagaagctt tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg
    10321 ttcatccagt aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata
    10381 acgaataaaa aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt
    10441 aatatcatta gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt
    10501 tagatgaaag tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg
    10561 ataaaaatga ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga
    10621 tggaagattc ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa
    10681 atgatgggag agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa
    10741 aagacgaata ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg
    10801 cagagtttta tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc
    10861 tacaacacta tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc
    10921 agcaaatgct attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg
    10981 atttattcgg tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag
    11041 aaatcgccaa attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac
    11101 tgaaaaaaga tggtattatc accgttaaag accatcatat cgttattttg aatatggact
    11161 tgttagaaga caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc
    11221 tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa
    11281 taccgagggt cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact
    11341 ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt
    11401 gtgaccgagg agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg
    11461 catcaagtaa taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac
    11521 ttatcaccgt agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca
    11581 cgatttttgt cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg
    11641 ccgccaccgc caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt
    11701 ccaggaggtg ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg
    11761 gttttttcag ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat
    11821 ttcaattgat aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa
    11881 acaacagcaa tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt
    11941 tcttcgtcta cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta
    12001 gacaggtaag cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca
    12061 tctaagatcg aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa
    12121 atgtagattt cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg
    12181 actgcgtctc ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc
    12241 gttacgatca agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa
    12301 gattggattt gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca
    12361 tgagtagcca ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc
    12421 atcatatcaa gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag
    12481 cggtcagtcc caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc
    12541 cattcattgc gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc
    12601 ataccggcag taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac
    12661 cggtcaatgt tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta
    12721 attcagattg aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga
    12781 acccggctaa atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc
    12841 cttcgaccat cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag
    12901 aaagcttgcc tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta
    12961 tccgccatat gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg
    13021 tagcccaata agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag
    13081 ctttgggaac aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt
    13141 tctcgacagc aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata
    13201 gtacgacggg attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga
    13261 tctctgcacc acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt
    13321 cgtccgctgt atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca
    13381 tatcggcaag ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt
    13441 tgctttcttc gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt
    13501 cttctgtata gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag
    13561 tcaacggacc gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt
    13621 tctaaataaa ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt
    13681 tagggatgcc gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct
    13741 taggctgaac gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat
    13801 tttctgtccc gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat
    13861 tttgtttttc tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat
    13921 aagaaccatt tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt
    13981 cattttttcc ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta
    14041 cgctaggatc acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat
    14101 cggtgtaaga aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc
    14161 aagaatcatc caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat
    14221 aagaaatccg ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga
    14281 attcagctaa atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga
    14341 ttgcgtttgt ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc
    14401 gtaagaataa ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga
    14461 atttgattcc ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga
    14521 cgccgccaaa tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga
    14581 acatggaagg agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga
    14641 tgatttttcc accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc
    14701 cgtctgttaa aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga
    14761 ctttgacacc ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta
    14821 gccattgatc tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa
    14881 aaaattcctc ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag
    14941 gaagatgtga aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta
    15001 aatacctgaa aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga
    15061 gatagtgaat tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct
    15121 ggcgttcgca gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa
    15181 atgctattgt ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg
    15241 gaaagacgag tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg
    15301 gtgagccatg caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg
    15361 tgatcgaaat cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc
    15421 gggcaaatta tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata
    15481 tgctttcaac aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg
    15541 tcatattcat cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga
    15601 ctcagcgatt tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta
    15661 tcttgaaaga atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg
    15721 cagaaggcgg gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag
    15781 gaacaattac tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg
    15841 acagttactt gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc
    15901 aaattctcgc agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc
    15961 gtgacttgct gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca
    16021 gaagtacatt tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa
    16081 agattttgag cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg
    16141 aagtcgctac agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag
    16201 catttgcaga tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg
    16261 aaatcaagga actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc
    16321 aaacaaatca taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag
    16381 ttctgcggga agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc
    16441 tgatgagtct atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg
    16501 gacctacagg actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg
    16561 acgaagaatt gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc
    16621 ctgatcctgt ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc
    16681 aggcacaaga agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg
    16741 agttgcttcc tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac
    16801 tgtttggctc cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg
    16861 atgcgtggaa tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa
    16921 atggcagaag cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa
    16981 ttggtcaaag cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa
    17041 gtcgttgatc ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat
    17101 gcgatcgcac aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg
    17161 ccaggatttt agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg
    17221 aaactgatcg acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc
    17281 gccttttata cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta
    17341 agtgtaaaac gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc
    17401 tgcgaaatat gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg
    17461 aaagatgtaa tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat
    17521 ggtgtgttat caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg
    17581 cagcggctgc atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag
    17641 ggagaagcaa cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact
    17701 cgtctagcac atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta
    17761 ttgaaagctg tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata
    17821 caatataaaa gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc
    17881 gaggggcctg atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa
    17941 gaagtagcaa aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa
    18001 gagatccgag cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca
    18061 ctgctttatg cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca
    18121 aaagggaaaa tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca
    18181 ggccgacgca tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact
    18241 acgccggatt ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag
    18301 aaaaacagac aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt
    18361 gtacgtcatg cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat
    18421 gcaagaaaaa gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat
    18481 ccgcaatttt ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc
    18541 aagacaaaga cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa
    18601 aactatcatc gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg
    18661 aagatgaccg tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc
    18721 aatttgtatc tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag
    18781 tagaagtaga agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat
    18841 tttaggagga gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg
    18901 tatcagcagc ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa
    18961 ggggagaaag gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt
    19021 acccaaatga aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag
    19081 aaagaacatc cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag
    19141 atcagacgtt tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt
    19201 ttgatcaaag aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg
    19261 gaagagcccc ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc
    19321 cctactattc aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg
    19381 atccatcgat tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta
    19441 acaaacagtt taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag
    19501 gacttgattt atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat
    19561 gtccaaaaaa aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct
    19621 gtcttgatgt tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc
    19681 aaagtgaaac agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc
    19741 agcaatgtca gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt
    19801 gccgcaaagg atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata
    19861 gagatgcagg tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca
    19921 aggtattagt cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag
    19981 agattgatcc caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag
    20041 ctgaattaga aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa
    20101 aagaaaaagt gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg
    20161 atcgaaataa attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg
    20221 taaaagatct ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg
    20281 atgaagcaaa gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt
    20341 ttttaggcga tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga
    20401 atccaacaaa aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg
    20461 aagaatacga gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg
    20521 atggtaaagg gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga
    20581 ctggacggga aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag
    20641 gtgagatgaa tggataagag atcattatac gacggattga atcaattaga atcagagttg
    20701 agaaatacat taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa
    20761 aatacaactt tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa
    20821 gttgcacaag ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt
    20881 gaaaaaattt atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac
    20941 aatgacgagc cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca
    21001 ataacaagag gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt
    21061 gacagatgta taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg
    21121 taccgacgcc aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag
    21181 aatccgatct tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg
    21241 aaatccatac accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat
    21301 tgattgcttt gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt
    21361 ccatcagcga tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga
    21421 tctctatccc aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc
    21481 aaccattttt attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga
    21541 aaaagctaaa agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg
    21601 ctacattgac gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat
    21661 tgacaaaggt ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg
    21721 aagaacatcc agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc
    21781 cagtgacgga agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg
    21841 cttcaggcga aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa
    21901 aacaagaagt ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat
    21961 gaaagactaa tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt
    22021 tcttgtataa tgaataagag gtgaggatga tggagctgca atttccttta cataatgata
    22081 catcaagaaa aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag
    22141 atcatcctga gtttcgcgga catccattag taattgccag acaccccagt gacactggtg
    22201 ggcgtggagt agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa
    22261 tgagtgcaca aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc
    22321 ataaatacag cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg
    22381 tggaaccagt tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca
    22441 agtcagccat caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa
    22501 catgttctgc gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa
    22561 aacctagagg aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc
    22621 caattgaaaa atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga
    22681 tttatacagg aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa
    22741 aaatgggcta ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta
    22801 caagagagcg aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag
    22861 aagcagtgct gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag
    22921 tccaaaaaca tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga
    22981 ccaaaagagt gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa
    23041 gccttatatg ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt
    23101 tgacgaatct ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag
    23161 aaatataaaa acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt
    23221 tttatatcaa gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt
    23281 cccaaatcac aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc
    23341 tacttccgcg aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa
    23401 ggatatcgat ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa
    23461 aggtatagcc tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc
    23521 gtacgacttg cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc
    23581 cttgcacgta atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg
    23641 gtttgccata tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc
    23701 agccactaac gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt
    23761 agtcagcaat atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc
    23821 tcataaaaaa acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac
    23881 tcgaccacat aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta
    23941 ttttgataga gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt
    24001 gtcatagcct cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc
    24061 caactaaacg aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag
    24121 gacagctttt tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg
    24181 gccatgtacc atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac
    24241 atcacatgtt tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg
    24301 tttgaaatca ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag
    24361 atacccgatt ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg
    24421 gagcataggg tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt
    24481 ctcaaattcc aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt
    24541 tgtataatat tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg
    24601 gcagaaacgg tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata
    24661 ttcatccccc catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc
    24721 ttttgcttct gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa
    24781 tccaatgtag cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc
    24841 ggtacgtgaa agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg
    24901 aatggcttct ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg
    24961 gtcagacagt aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg
    25021 caaattcaat gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc
    25081 gtctttccac ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa
    25141 ttcaacgatt ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa
    25201 tgctagttgt ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa
    25261 agtgaagatc agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg
    25321 aatctgtaaa attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc
    25381 taatgaagtg aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa
    25441 actttgcata gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg
    25501 ttctggcgaa tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc
    25561 aaaaggagca agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc
    25621 tacattggca ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc
    25681 gacttcggca ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca
    25741 gcaattcgtc atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt
    25801 ttgatgcgca tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat
    25861 cccggctgtc agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt
    25921 cacgacagtt tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt
    25981 actgcgagca aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc
    26041 gtttttttct agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc
    26101 agcaaaccgg cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata
    26161 agtaagtggt gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat
    26221 cttttgttca tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt
    26281 ctctggctgc tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc
    26341 ctgctaaatt gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg
    26401 ctatatatag tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt
    26461 ttattgtgaa aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttatttt
    26521 tgctgaataa aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca
    26581 taagagcaaa ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag
    26641 aggaggagaa gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag
    26701 caaactttct cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt
    26761 tgttgggatt atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac
    26821 gatcaatttg cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa
    26881 aaacagtgag ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa
    26941 tttgatcgat accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat
    27001 gactaaagag gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga
    27061 actgctcaaa cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc
    27121 tagagcactt gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga
    27181 ctcgaagtcg gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc
    27241 aacgatactg ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt
    27301 tattaaagat ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt
    27361 tgagaaagta atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata
    27421 aactagccgt taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt
    27481 tgactttatc cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag
    27541 tcagaagagc aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca
    27601 ttactgttgt tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc
    27661 aaaaggaaat aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat
    27721 atgtgttaga aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta
    27781 ttattttttc caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta
    27841 gtccattttt tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc
    27901 ttttagcagt atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt
    27961 ttggagaaaa agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac
    28021 tcgcagtcat cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg
    28081 aaatcacttc atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt
    28141 ttttaatttt tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt
    28201 tatttttgat catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa
    28261 tgggtaacac gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca
    28321 ttctcggaat ttctttaatg acaattggcg gcacggctag tatctctact attttatcct
    28381 accaggagaa agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa
    28441 tcaagccgat tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata
    28501 aagtcattgg gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc
    28561 tgatcacgga aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt
    28621 ctttgaaatc acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata
    28681 tttcattata tggatctagt attggactgc cagataatca agaattgaag attcaacaaa
    28741 tgcttccaag tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag
    28801 atgataccgt ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat
    28861 caggtggaaa taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc
    28921 tcaatgctgt ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca
    28981 caaagaaact tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt
    29041 tgaatttctc ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata
    29101 tttttgtcac actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag
    29161 tccggcaatt agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg
    29221 ggattccaca aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc
    29281 cacccatgat attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata
    29341 taaaaactgc cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc
    29401 ttttgcttta tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg
    29461 aataagacaa gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt
    29521 tgtgacacga tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat
    29581 tataaagaaa aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg
    29641 ttagtaaaaa aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac
    29701 actttgtgtg aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa
    29761 ttgaattatt ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc
    29821 ctgatttatc tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt
    29881 taggaggaag agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa
    29941 tgaacctgat cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca
    30001 agcatcagac gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt
    30061 cgtttttggc aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg
    30121 ccatgcctat ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg
    30181 ggtggctaaa ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt
    30241 tcctgtcata acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc
    30301 cagaaatagc gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg
    30361 agaaccaaaa ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat
    30421 ggaagacttt gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga
    30481 tttatcggta ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa
    30541 taaagcagtt gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa
    30601 tgtgtgggtt tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg
    30661 agaaaatgtg ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac
    30721 tgcagaaagt ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac
    30781 agaagcaact gtagctattt caagtattca gattgaccag catgtactag atagttatga
    30841 ccgactaccg attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa
    30901 acctgtttca aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg
    30961 ttacctgaac aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag
    31021 ttaccataca ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg
    31081 tatggatcag caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta
    31141 tctgctccaa gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa
    31201 agtacagcag ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca
    31261 gttgactaaa tctatcaaag aacaattact agaagttgta atggattata tgattcctca
    31321 gaagttccat tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa
    31381 attaacagct gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg
    31441 ttttattttt ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg
    31501 gaaaaaagat taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt
    31561 ggaggtaatt attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata
    31621 cttgtatggg gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc
    31681 gccgttattt tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat
    31741 ggaaaagcat cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa
    31801 atgatcatgg aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag
    31861 tttctgctgt tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa
    31921 aaagatcagc tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc
    31981 caaaatattt ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta
    32041 ctgcttcctg tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta
    32101 ggttatatgt acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt
    32161 gcagtcggta caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt
    32221 ttatcatgga atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc
    32281 cgtgattatg tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc
    32341 aagatcgtaa tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat
    32401 ggattgacgt ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca
    32461 gatgcatgga tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg
    32521 catggcttag ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca
    32581 ggatttttag ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa
    32641 aggagaaaag gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag
    32701 atgaagtaag aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag
    32761 catctgttca attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg
    32821 aattcgagcg agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga
    32881 agggttgagt atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg
    32941 cttactgttt gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat
    33001 tagacgtgcc tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta
    33061 tgcattatct gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag
    33121 tccatttcat ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct
    33181 gggagcaccc gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc
    33241 tatgaaaaac aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt
    33301 aaaaactgat tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc
    33361 aatgaagaaa gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa
    33421 agtaaaagag aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc
    33481 tttacctgaa tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga
    33541 agtattcagc aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg
    33601 attaccaaaa caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg
    33661 agaaacgacg aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc
    33721 ccacgagcca gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt
    33781 ttctgatttt cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat
    33841 catcccaccg gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca
    33901 aggatttgcc aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga
    33961 ttttgtcaat caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa
    34021 aggctggttg gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc
    34081 tagtaagtat catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga
    34141 taaactacaa ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt
    34201 gaatattctg aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg
    34261 gtataatcac aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag
    34321 ctacttattg atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa
    34381 gtattcagag aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt
    34441 gtgattgcca aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac
    34501 gaagttggtt ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt
    34561 ggaggttggt gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg
    34621 aaaatcgtcc acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga
    34681 aataaacctt atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa
    34741 gccaaagagt ggggcatacg tcctggagat atggtcactc catatattga atacaaacga
    34801 atgaatggca gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt
    34861 tctctgcgag tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga
    34921 agcgatgtgc aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat
    34981 cccgatattg cttttgcttt agatactggt acagcaggag acactccagg aatgacacca
    35041 aaagaagcag attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg
    35101 atcccgcata aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca
    35161 ttccaatata cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga
    35221 gatggtgttc cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca
    35281 gtgattcatg aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg
    35341 cttgatagtg aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta
    35401 tttctgaata tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt
    35461 ggcttgtttt cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca
    35521 gtccttttcc ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac
    35581 ttttattcgc attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca
    35641 gaccaaaaag taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg
    35701 gcatcaattt gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca
    35761 tgtttttaat cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac
    35821 ctacgggtgt catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga
    35881 tgatttttgt ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat
    35941 ggacaaagtt cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt
    36001 tattgaaaga tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt
    36061 tgctaggagc aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt
    36121 cagttgcaga attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta
    36181 cttagcatgt aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg
    36241 agggggaatg tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa
    36301 atcaagggac aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag
    36361 gtagtgctac gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga
    36421 tccttagcca ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc
    36481 aacttcatcc taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt
    36541 ccattcactt tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag
    36601 ctgaagaatt aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag
    36661 tatgttacgc catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg
    36721 attcaggtta tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag
    36781 atacctatct ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg
    36841 gagaacttgc gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg
    36901 gtgatttaga acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag
    36961 aacttgctgc agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca
    37021 aatgaaaacc acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg
    37081 cctttagatg aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga
    37141 aaccaaaata cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt
    37201 gtccgtcgct taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt
    37261 tttatcatgc ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc
    37321 atccaagcac ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta
    37381 gtcattgatg gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt
    37441 gctcatggaa caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta
    37501 aaaaaagaca aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc
    37561 aaaccatttc ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc
    37621 ttgttgaaaa ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat
    37681 gaagattgga aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat
    37741 tacgggaagt ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt
    37801 gaagtccatt tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc
    37861 ttcggactag gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa
    37921 gcatctctat caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa
    37981 ccagaagaat tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg
    38041 gattccgcta ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga
    38101 tcaatgaaag tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc
    38161 atcatcatta agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat
    38221 aggtttgtat cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg
    38281 tgtatcgaca aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag
    38341 gaatgctcta aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct
    38401 tcgggaagat ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa
    38461 cgcgctaaga taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt
    38521 tttgcttctt ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt
    38581 aaatcttgca gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg
    38641 atatgctgtc gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa
    38701 tctacttgtc tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata
    38761 ataacgatta gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata
    38821 cctaatgccg tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt
    38881 gtacaagcta cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg
    38941 actaaagtgc ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca
    39001 ggagtaaaag ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa
    39061 attccgtacc attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct
    39121 gacgttaaga taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata
    39181 ccacttaaaa tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag
    39241 agaataagtg agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag
    39301 gtccctatag cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa
    39361 taaataaaac taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca
    39421 tagttctgat aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt
    39481 aaatctatca cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg
    39541 tataaaatct tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa
    39601 tttgcttcgt ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc
    39661 acggtaggaa tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta
    39721 tagaaaaccc acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata
    39781 aaataaaaga aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc
    39841 ca
//
LOCUS       DAZUMV010000013        39441 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000013 DAZUMV010000000
VERSION     DAZUMV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.013"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..658)
                     /locus_tag="ACG8FK_000599"
     CDS             complement(113..658)
                     /locus_tag="ACG8FK_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5096236.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(670..1362)
                     /locus_tag="ACG8FK_000600"
     CDS             complement(670..1362)
                     /locus_tag="ACG8FK_000600"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS5096237.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1381..1665)
                     /gene="macP"
                     /locus_tag="ACG8FK_000601"
     CDS             complement(1381..1665)
                     /gene="macP"
                     /locus_tag="ACG8FK_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS5096238.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1678..2241)
                     /locus_tag="ACG8FK_000602"
     CDS             complement(1678..2241)
                     /locus_tag="ACG8FK_000602"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5096239.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2469..3131
                     /locus_tag="ACG8FK_000603"
     CDS             2469..3131
                     /locus_tag="ACG8FK_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS5096240.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3255..4451
                     /locus_tag="ACG8FK_000604"
     CDS             3255..4451
                     /locus_tag="ACG8FK_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS5096241.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4552..5928)
                     /locus_tag="ACG8FK_000605"
     CDS             complement(4552..5928)
                     /locus_tag="ACG8FK_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5096242.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5965..6831)
                     /locus_tag="ACG8FK_000606"
     CDS             complement(5965..6831)
                     /locus_tag="ACG8FK_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5096243.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6920..7945)
                     /locus_tag="ACG8FK_000607"
     CDS             complement(6920..7945)
                     /locus_tag="ACG8FK_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5096244.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7963..8637)
                     /locus_tag="ACG8FK_000608"
     CDS             complement(7963..8637)
                     /locus_tag="ACG8FK_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5096245.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8821..9714)
                     /locus_tag="ACG8FK_000609"
     CDS             complement(8821..9714)
                     /locus_tag="ACG8FK_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5096246.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9788..11485)
                     /locus_tag="ACG8FK_000610"
     CDS             complement(9788..11485)
                     /locus_tag="ACG8FK_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5096247.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11478..12791)
                     /locus_tag="ACG8FK_000611"
     CDS             complement(11478..12791)
                     /locus_tag="ACG8FK_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5096248.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12791..17060)
                     /locus_tag="ACG8FK_000612"
                     /pseudo
     CDS             complement(12791..17060)
                     /locus_tag="ACG8FK_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
     gene            complement(17357..17875)
                     /locus_tag="ACG8FK_000613"
     CDS             complement(17357..17875)
                     /locus_tag="ACG8FK_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5096249.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17877..18494)
                     /locus_tag="ACG8FK_000614"
     CDS             complement(17877..18494)
                     /locus_tag="ACG8FK_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5096250.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18491..19345)
                     /locus_tag="ACG8FK_000615"
     CDS             complement(18491..19345)
                     /locus_tag="ACG8FK_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5096251.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19342..20253)
                     /locus_tag="ACG8FK_000616"
     CDS             complement(19342..20253)
                     /locus_tag="ACG8FK_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5096252.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20294..21628)
                     /locus_tag="ACG8FK_000617"
     CDS             complement(20294..21628)
                     /locus_tag="ACG8FK_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5096253.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21836..22666
                     /locus_tag="ACG8FK_000618"
     CDS             21836..22666
                     /locus_tag="ACG8FK_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFS5096254.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(22910..23159)
                     /locus_tag="ACG8FK_000619"
                     /pseudo
     CDS             complement(22910..23159)
                     /locus_tag="ACG8FK_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23350..23544)
                     /locus_tag="ACG8FK_000620"
     CDS             complement(23350..23544)
                     /locus_tag="ACG8FK_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096255.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24049..24234)
                     /locus_tag="ACG8FK_000621"
     CDS             complement(24049..24234)
                     /locus_tag="ACG8FK_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096256.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25084..25425)
                     /locus_tag="ACG8FK_000622"
     CDS             complement(25084..25425)
                     /locus_tag="ACG8FK_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5096257.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25425..25655)
                     /locus_tag="ACG8FK_000623"
     CDS             complement(25425..25655)
                     /locus_tag="ACG8FK_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5096258.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            25942..26280
                     /locus_tag="ACG8FK_000624"
     CDS             25942..26280
                     /locus_tag="ACG8FK_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096259.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26485..27047)
                     /locus_tag="ACG8FK_000625"
                     /pseudo
     CDS             complement(26485..27047)
                     /locus_tag="ACG8FK_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27779..28360)
                     /locus_tag="ACG8FK_000626"
     CDS             complement(27779..28360)
                     /locus_tag="ACG8FK_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5096260.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28543..29169)
                     /locus_tag="ACG8FK_000627"
     CDS             complement(28543..29169)
                     /locus_tag="ACG8FK_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5096261.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29191..30522)
                     /locus_tag="ACG8FK_000628"
     CDS             complement(29191..30522)
                     /locus_tag="ACG8FK_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS5096262.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30677..31078)
                     /locus_tag="ACG8FK_000629"
     CDS             complement(30677..31078)
                     /locus_tag="ACG8FK_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5096263.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31164..31370)
                     /locus_tag="ACG8FK_000630"
     CDS             complement(31164..31370)
                     /locus_tag="ACG8FK_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096264.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31465..32967)
                     /locus_tag="ACG8FK_000631"
     CDS             complement(31465..32967)
                     /locus_tag="ACG8FK_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS5096265.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(32985..33440)
                     /gene="argR"
                     /locus_tag="ACG8FK_000632"
     CDS             complement(32985..33440)
                     /gene="argR"
                     /locus_tag="ACG8FK_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5096266.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33538..34155)
                     /locus_tag="ACG8FK_000633"
     CDS             complement(33538..34155)
                     /locus_tag="ACG8FK_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5096267.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34376..35749
                     /locus_tag="ACG8FK_000634"
     CDS             34376..35749
                     /locus_tag="ACG8FK_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5096268.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            35824..36936
                     /locus_tag="ACG8FK_000635"
     CDS             35824..36936
                     /locus_tag="ACG8FK_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5096269.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37008..38645)
                     /locus_tag="ACG8FK_000636"
     CDS             complement(37008..38645)
                     /locus_tag="ACG8FK_000636"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS5096270.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(38813..39421)
                     /locus_tag="ACG8FK_000637"
     CDS             complement(38813..39421)
                     /locus_tag="ACG8FK_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5096271.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11351 a         8386 c         6837 g        12867 t
ORIGIN      
        1 gaataagttt ttttaaaata acgtataaaa aagagcataa aacaaagtcg tttgttttat
       61 gctcttcttt tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa
      121 tttctgcgcc tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga
      181 aacttgccac ggcatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg
      241 ctgtatgcaa cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc
      301 gttctcgcaa acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta
      361 tccaataaac attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca
      421 attcccgtcc acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa
      481 aggaatcttt tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa
      541 acttctttgt atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca
      601 gctttccatc ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct
      661 tagtcctcct tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc
      721 tgcgtcttcg ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat
      781 tgcacgtacg atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc
      841 ttccatttcg caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg
      901 cgagtcgaca aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc
      961 ttttgctgct ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat
     1021 tcctggtaat tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac
     1081 tttgtctgat ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc
     1141 tgtattgatc accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc
     1201 taagactttt cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga
     1261 aataaataaa gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat
     1321 ttttacttct tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt
     1381 ctatataaat gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga
     1441 tttcatcaaa aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt
     1501 gattggttta ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg
     1561 ctgataagct gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg
     1621 tcgcttgcga agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta
     1681 ctttcctttg gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat
     1741 caactgatct ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac
     1801 ttcgtcttca tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat
     1861 atgaaggact tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga
     1921 cttcgcacga tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg
     1981 tccttctcct ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt
     2041 tcgaaattgt ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc
     2101 gccaggatga aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc
     2161 tacagcaaca tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc
     2221 aaattgctca ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt
     2281 ttcatttttc aatttttctc aaaaactcct tattagttta ccatagataa accttagatt
     2341 ttctctaaat atagaaaatc cttctttagt ccgatactct ttatagtagt ccgtatgcta
     2401 aaataagtat gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag
     2461 ggtttattat gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct
     2521 ttgtccgaaa caatagtttt tcttttgttc taggactttt attgataggt gtagttttag
     2581 ttttgtgggg actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac
     2641 tgactgacga acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat
     2701 tttttcgaca aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc
     2761 agcagacagc caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag
     2821 ctttattcaa agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt
     2881 atacgcatct gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg
     2941 agatcaaaaa caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc
     3001 tgtcagcttt aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg
     3061 acgtagagat atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac
     3121 aaaaggatta actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct
     3181 gtttcgagta ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca
     3241 aggaggaatt ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg
     3301 atacacttga agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt
     3361 cacaacaagc ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca
     3421 aactaccggc ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg
     3481 atgcacagtc tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac
     3541 aatcgatgtt gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg
     3601 aactgatgta tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca
     3661 tcttccagcg tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc
     3721 aaaagattgg tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc
     3781 tgctaaaaga taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg
     3841 cactgaatat ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca
     3901 ttccagaagc gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca
     3961 atgattacac gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta
     4021 gacagatcac gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct
     4081 tggctgaaaa gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg
     4141 tgatcgcatt gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg
     4201 aaacaacaaa cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa
     4261 cagcgaaaga aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg
     4321 atttgatcga aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc
     4381 gtcatgcaga agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt
     4441 taacccaata gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac
     4501 aactgttgat gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc
     4561 attcatcttg ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac
     4621 gataccgaac acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa
     4681 gatggagacg attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt
     4741 gccaaacatt tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc
     4801 catgatattc gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc
     4861 tgccacacca gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt
     4921 cataaactta ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata
     4981 catcatcgag atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc
     5041 cactgttcca tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc
     5101 agtatacgga acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat
     5161 cagtatcgaa ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc
     5221 tgccattgca acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag
     5281 cacaccggaa aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat
     5341 tcctacaatg ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa
     5401 acgtaatgct ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac
     5461 cgcaatcaag tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg
     5521 aagttcggcc cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac
     5581 agaggaaacc gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa
     5641 ttcaccaaat acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc
     5701 aaattctgcg aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag
     5761 tgacagtgct ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt
     5821 accgatgatc gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat
     5881 ctcactcgta tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc
     5941 ccattttagg ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc
     6001 taaaatgact aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc
     6061 gccaaggatg aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa
     6121 gccaacagct aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca
     6181 taataatcca cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc
     6241 aaagaagctt gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc
     6301 caaattcgcc cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa
     6361 tgctctaaaa cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc
     6421 atcttgtcgg gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta
     6481 tacataatct ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa
     6541 aattaattga aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc
     6601 atggaactgt ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa
     6661 aatccaaggg gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc
     6721 accgatcgtc attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa
     6781 accaatacta ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct
     6841 ccttttattt cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt
     6901 tttcgtttct aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata
     6961 aagtcattgt tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt
     7021 cggccagatt ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac
     7081 catttctccc agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga
     7141 tccctgaatc tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga
     7201 tgtttccgtg atccggcgta tatttgatgg cattactaag caattggttg aagataaaag
     7261 cgatccattt agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat
     7321 ttttttggat aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc
     7381 catattcttg gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt
     7441 gttctacgta ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt
     7501 caatatcgaa ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt
     7561 catggaccca tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca
     7621 ttacttcttg gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt
     7681 ctgtcctccc accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt
     7741 ttccccacca tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc
     7801 cctcaatcaa agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc
     7861 acatcacaaa gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat
     7921 ctttcaaata tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc
     7981 ctgtcctact tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa
     8041 ccgattgatg ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc
     8101 tcgcagcaat ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag
     8161 acgatattca tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc
     8221 aacattcaac gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta
     8281 agaacggcga agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt
     8341 cacaaaatca tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct
     8401 ggagataaaa atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc
     8461 atccaaaacg ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc
     8521 tgagaacact tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc
     8581 ctccatgatc aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa
     8641 aaatccctcc atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt
     8701 ggcttacctg gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta
     8761 atcgcgtttg tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta
     8821 ttatctgtta tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc
     8881 gaaaagcgtt agcactagca atatacctag aaggagtata agtaatcgat aaagatggta
     8941 tttttttgtc tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt
     9001 cactagtaat cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc
     9061 ctgcttttct ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac
     9121 gacttcataa gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat
     9181 ttcttcaaga tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga
     9241 atgtgtactc tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc
     9301 taacaaaaga tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata
     9361 gatggctccg atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac
     9421 cgcttgctca aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag
     9481 cgtctgattt ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg
     9541 ttcaagctgc ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc
     9601 tgctaaaaat ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa
     9661 ttgtaataaa agactgattt gggtttgagg tctattacct attttctttt tcattttctt
     9721 caccttctta tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt
     9781 ttgttgctta tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca
     9841 tcaaacttat tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg
     9901 acccgcgttt catattcggt actctttctg gccctggatt ttctactcct acgatctgaa
     9961 tttcttcatt atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg
    10021 gaagtacata accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc
    10081 cttgaacctc aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg
    10141 cccactcaat tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa
    10201 atattgcgta ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt
    10261 cactgcttga tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg
    10321 gctctactcc ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt
    10381 tgttatggaa atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta
    10441 aatctcctcc acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt
    10501 cttctgtcaa aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc
    10561 cttttgttaa aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac
    10621 caactgtagg cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga
    10681 cagaatcgat gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt
    10741 ctagatcaat ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc
    10801 taaggtcgct ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat
    10861 acaaatgcac atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca
    10921 ccatatcagg catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat
    10981 gagctggcga ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt
    11041 ccgtcacttg gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa
    11101 tacttgtcag tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa
    11161 tcgcttcggc agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca
    11221 aatcgtaata tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt
    11281 caccaaaatc cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac
    11341 cttttttgtg taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga
    11401 ctagttttcc gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc
    11461 ccgtgacaat cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca
    11521 gcacctaaca ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc
    11581 gggaatacgg accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt
    11641 ttgtccgttg tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca
    11701 gagactggaa ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat
    11761 tgtggttggc cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt
    11821 gcttcatcca tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa
    11881 ggcaagaaac gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca
    11941 tttttgtcat tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta
    12001 gcaggagcca tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca
    12061 gcaccttcta atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag
    12121 tatggatctc ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg
    12181 gttgattctg tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg
    12241 atatcaacat ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca
    12301 tcccgatctt ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca
    12361 accgtatctt tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt
    12421 agctgacgga tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc
    12481 atgttttcag cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc
    12541 gctccattta gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag
    12601 atagccaagc catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc
    12661 atccgtttat gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca
    12721 tgtggaagaa ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac
    12781 agctttttca ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg
    12841 ctcctgcaag cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc
    12901 ctgtagcagg caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat
    12961 ttgtctccaa aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt
    13021 ttggataata cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat
    13081 cattttctat gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt
    13141 ttctatagct gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg
    13201 aagcattgaa tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat
    13261 actctcctgc actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat
    13321 tcatactgtc aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta
    13381 cttgtagtac accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga
    13441 tgaccgtatc ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat
    13501 cagatagccg atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca
    13561 gatgcagagg ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat
    13621 agaagtatcc gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt
    13681 atgctcgata cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat
    13741 ctaatgtatg gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat
    13801 tgtaagcagt gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt
    13861 gccaattttc ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc
    13921 gcaagatcca tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa
    13981 tgattttgta ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg
    14041 gctctccgat tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat
    14101 ctactgaata gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc
    14161 ctttcgctac tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact
    14221 ctgcgccatt gattggcata ttactgcctt caattagttt attgatattc atgtggattg
    14281 gcttgaattc atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta
    14341 tgttaggctc ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat
    14401 ctttaccttc cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt
    14461 gacctaacat ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat
    14521 ctactaactg ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg
    14581 ctccagtttc atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg
    14641 tagttttact tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg
    14701 caaattcgac tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac
    14761 ggcctttatg ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat
    14821 cattctcata atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc
    14881 cgtcttgagc tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac
    14941 ctaaagaaat cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag
    15001 tcgccactac ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt
    15061 ggtacttcat gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc
    15121 cagctccaaa tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg
    15181 ttctcttgaa ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg
    15241 cattgtaata tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat
    15301 ttcgtccaat agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa
    15361 tggagaagat acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa
    15421 ctgtgttgat cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag
    15481 aagaaccatc accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac
    15541 gatagtgtac atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat
    15601 gagtaaaccc tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt
    15661 cattatcttc agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc
    15721 tcgtacgata acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa
    15781 cgattaggat tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat
    15841 agccattttt aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg
    15901 tattctcact tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga
    15961 tttgctcatg gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa
    16021 tatccagact cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg
    16081 cagcattttt agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat
    16141 ctagaacaaa aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta
    16201 gatagacatt gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg
    16261 gcagaacagg ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt
    16321 caaaattccg ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt
    16381 cgcctgcagt tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt
    16441 ttgtataatc actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt
    16501 gatagcgttc attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa
    16561 acttcatcta ttctttcatc aaccaccact tgaagaggta gttacccttc gttcacatcg
    16621 gggatctttt ctgtttcaaa aatcaatgtg ccggtttctg cttcttttga ataaactttt
    16681 ccgacatacc attcttgacc atcgatcgtc tcgactttta ggtcttctcc atcttcatca
    16741 taggaggata gacctgttcc acgaacgttt tttacggtac caatcccggc ggctgccgca
    16801 tcgattgcta aacgtattct accttcattg gcactttcct ttttttcaaa agccagatgc
    16861 cattcaaggg tttcttcttt ttcaatatac ttcccttcaa tttttgcata gtcctcatct
    16921 aagaatacct catcctcctt tgtttctgtt gactcagcag ctacatccag ggtcgtcata
    16981 tgatagactg actggaaata tggtccaaat agcaaactga cagttactaa ctgcaaaagc
    17041 ttcaatttta accctttcac atccttatac tcctttcaaa aatatttgtc ttctttcaca
    17101 acaatcaaca aaacaatgcc catctaatta taatttaatt taattttaat tttaaatcaa
    17161 atgatatcct ttttagttaa aaacaccaat tatacaacat ttattattaa aattaaatta
    17221 taaaatttta taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca
    17281 actacgcttt cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt
    17341 tattcgtaca attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa
    17401 tagctcctcc taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga
    17461 tcaaacaagc aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag
    17521 ctactgataa aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac
    17581 ttgtgtaaag gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt
    17641 tgataaaaag caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat
    17701 attctctagt atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac
    17761 cattagagat attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt
    17821 gcttggcgaa cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat
    17881 tcctcctttt taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa
    17941 aacaagaaac tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca
    18001 agaaggatca acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag
    18061 aaaaatgcag taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta
    18121 aagagaagcg cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt
    18181 cccccacccc aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt
    18241 gctaaactat cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt
    18301 gaaccgagct ttttctcttc attttttttg taatgataaa tcatatacag aactgttcga
    18361 cttgcagttc ctaatgtgaa aacaggcaag acgcacagga accatagtag ctgtaaatgg
    18421 ataagacgtg gaatccattc aatgatttct aagaaataat cattggcttt tttaccaatc
    18481 gatcgattca tcatcctttt gaacctcctg tcaaatccat cccttcaatg aaataacgtt
    18541 gtgcaaaaaa gaacagcaaa atcgtcggga tcaagacaag tgttgctcct gccatcagat
    18601 aattccattg tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt aacgtgtatt
    18661 tttcttgatc ttggatatat aacagaggac ctaagaaatc attccaagct gcgttgaagg
    18721 aattgatcgc aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa tgactccaaa
    18781 tatacaaatg attcgctccg tcgatttcag cagcttcgat caattcatta ttgatcgata
    18841 agtaaaattg acgcatcaga aatatattga acgcattgcc gaagaacgat ggaacgatca
    18901 ggggcaaata agtacctacc cagccgatct tggagaataa tacatattga gggatcatcg
    18961 ttacaaaccc cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc tttttcccag
    19021 gaaaatcaag cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca ttaccgatca
    19081 ctactagcac agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga aaggcggcaa
    19141 tcgtatccag atagttttcc catacgattt tttctgggaa aaaggtcgga ggataacgaa
    19201 cgatttcttc cattggtttc aatgaagtaa acaccatcca aagcaatgga atgatcaaaa
    19261 tgattcctat tactgtcatg aggacataac ttagtatttt ttttgtttgc cgattcacac
    19321 tggtcttttt gaaataatcc atcatttgcc gtcacctcca gaataataga cccacttagg
    19381 tgcaagcttc aaattgacaa acgtcagaac caacacgatc agaaacagga tcattgacat
    19441 agccatcgca taacccatat caaaagctac gaacgccttg ttccacatat gcagattata
    19501 aaaaagcaaa gaatttgcag gtccgcctgt tccatttttc gtcatgacat aagcttcttg
    19561 aaaaatttgg aaagaaccga tcgtagaggt gatcacatcg taaaaaataa taggagtgat
    19621 taatggcaaa gtaatacttt tgaactttcc ccaaacgcct gctccatcga tttctgctga
    19681 ttcatataaa gatctaggaa cattttgtaa tgtagctaag taaagtagca ttgctcctcc
    19741 agaactccat attttcatca aaatcaacgc aggtttcgtc cattgtggat caaacagcca
    19801 agctggtcca gggatatgga accagcccag aaatgtgttg atcaatcctg cagatggaga
    19861 aagcagctgc atccataaga tgtagactgc tactcctgat aagactgccg gtaaatagaa
    19921 agctgtacgg aatacactga ttcctttgat tttttgattg agtactgtgg ctaaaaacac
    19981 actgaaaatc gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt tgtataacga
    20041 tttccaaaat aaatcatcgt gaacgaacat tcttttgaaa tttgctagac caatgaaatt
    20101 catcttggat gtcaaattat aatcggtgaa actaccaata actgaaaaaa gcataggccc
    20161 tagcataaat accaagaacc cgataataaa aggcgagata aataaatagg cccatcttgc
    20221 ttcttttttc tttcggatag ataaacgctt caaattttct tgcctcctct gtacctagac
    20281 agtcaaaaat ggattattga ttttgcttga ctaagttttc tactgctttt tgtgcatctg
    20341 ccaatccttc ttttggtgat tttgttccta aaagaatctg gtcaatctgt tcattcacta
    20401 atgtcgtgta atccggagca tctaaaggaa ctggtgtgat caccgtattt ttaaagtttt
    20461 cgtccgccat ttgatagatc atttttcctt tatccgttag gttgtcatta ttcactaaat
    20521 tctcattggc tgtcttgctc gccatgatat caaaactttt ttcgccgaat ttttcttgta
    20581 cttctggaga agaaagatat ttgatgaatt cataagaggc ctcaggatct tttgctccgt
    20641 atggtacttc caagacgaat ccgccacccc acgaccaatg gcctgaacct tcttcttttt
    20701 ctgggagctg catcacgcca aaatcaaaat catccggtgc attttccatc aaactagagt
    20761 aataattgat attttgggca cgcatggcta ctaatccgga aataaatgga tcagatacac
    20821 cagacccaaa ttcggcttct aatttattga tcgtattttt cccataataa tcttgccagt
    20881 cttggatcca ctccaaggct tccactttat ttttagtatc aatcttgact ttttcatctt
    20941 tgtcaaacca actagtcccg ttatctgcgt ttagcgccca tacatcagcc cctaaattcc
    21001 ataatggata aaaaccgatg cgtgtaaatt cttttccttc ttttttatct agtttacgtg
    21061 ctgtttcttc taattcatcc caagttgtcg gtagatcttg ttcactgatt ccagcttctt
    21121 taaataactt tttattataa aaaatgactt gtgtatctgt attgaatggg atcgcataag
    21181 gatcaccttc atattctact gtatcccaaa gctgcggata aaaatcctta gaagtgtctt
    21241 tttctacata tttggagaga ttagttgctt gctgtgcttc tgctctttgg gcaacggaat
    21301 ttatgtcctg tacaataaca tcgggtgcat ttccagctgt tattgcagat aaagatttag
    21361 tccagatgtc accccatggc tgatacttat aatttacatg gatctcgttt tgtgattgat
    21421 tgaaatcctc aatgatttct tcgatagctt cttttcgtgc accggatccc caaaaggacc
    21481 agaaatcaat attttttctt ccatcacttg ttttttcaga agaatttgct gcacttgaac
    21541 tggcatcgct tgcattactt gatccgcatc ctcctaataa aaacaatgct gaacttgcca
    21601 gcaaccctgt aaacaataat tttttcatat tttacctcca aattttaatg taaccgatgc
    21661 cggttttaat ataacgatat cattttgttc tgaaaataac aatttataag gtatgattct
    21721 aaaaaaatta ttaatgatac caatacttta tataattcgc gagcgctttc tttgaatttt
    21781 ttaatctttc gtagcagaat gagaatgtat taaaaataga ttaggaggaa ttttgatgtt
    21841 tagacaccag aaagaattgc agtttgaagc aaaaccagat cgtccgaatc cattaatcgc
    21901 gaaatatctg caggagctga tcggcggcca atatggcgag atgagtgtag ctatgcagta
    21961 cctgttccaa ggctggagct gccgaggaga agaaaaatat aaagatatgc tgatggacat
    22021 tgcaacagaa gaaatcggcc acgtggaaat gttggcgaca atggttgctc gtttattaga
    22081 gggtgcgcct gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa tcggtgccat
    22141 tatctctgga atgaatccac agcacgcgat cgtagctggc ttaggacctc gtcctgctga
    22201 cagcgtgggc aatccatgga gcggtgccta tatcattgcg agtggcaact tgttagcaga
    22261 ctttagagct aatttaaatg ccgaatcaca aggaagactt caagtagcac gtatttacga
    22321 aatgatcgat gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa gagatagcta
    22381 tcatcaaaat ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag gttcaatctt
    22441 agtcccaagt actttcccgc gtgacaaaga acgaaaagac attgcttatg acttctataa
    22501 cttctcagag ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag cactggatgg
    22561 cgaaggcgaa tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac caaaattaga
    22621 accagtaaca ccaaaaatgt atgggacacc tccattcaag aaataaatat ctacccgtag
    22681 aaacacccgt tttttgttcc acacgctttg tgagcaaaac tgactaaagt cattaatcat
    22741 aaccaccctt caaaagggtg gttttctcta gagctataag ctcaaatcac cggccagcgc
    22801 ctaaagacgc tggctttcat attttatgtt caagccatat tgcctttgcc agttttgccc
    22861 acctcttaaa aggtttatgt actactcctt agcccttaaa aggatcgtct tactctcgta
    22921 cactcagctt atctaacgca ctatcatgtt tttcttgttc ttgaatgtac tttttgatgg
    22981 tcgcttcatt ttgtcctact tttgtcacat agtatccttc cacccaaaag tgtcgatacc
    23041 caaatttata ttttagattt gcacaatctc ttgatgatct cctgaatatt ggctcgatat
    23101 tgattataaa ttatttttct tctatacttt ggtgtgaata caatgtggta tttacacatc
    23161 tactttgtac gggctaaact atttcccttt attgctccac ccgcatagca agtggttttg
    23221 tatttcaatc gctcggcgcg ctcaattagg tctaaagaca ataaatctaa aaacccgctg
    23281 cttcactgac agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga tctcttacgg
    23341 caatctattt tatttttttg attttttata aatggaaaat gcttcaagaa gtccaagaaa
    23401 taaagccgta taaaaacaag aacctatgaa atctttattt attagatatt caacagaaat
    23461 cccgataaca ctagccaaca atatatttat cagaagtgta aaataaggat gctttttata
    23521 gaatagaact agtgttgttt tcatagactc tcctttcttc atatttccaa ttaatcacaa
    23581 attcacggca ttactagaaa aattaaaggg gcgcagaagt ggaaaaatac tcttcgactt
    23641 tccctacaaa ccaggccatc ttgtgtttaa taactatacc aaatctaagg tacctctaaa
    23701 tctaaattcc atttgtggcg gtgacctcca atttttaact cttctatctc ccatccagta
    23761 tacataacct tgatacttcg accacttata tatcgaacac acaaatacct atccattgca
    23821 ggagtactac ctaacttagt tgctacttgc atctaaataa cagctgaaaa gataggactt
    23881 actcttagta aagtatctga actattaatt tttaaactat catttttatc ctcaactaca
    23941 tgcacatctg ttgcaacttc actttctgtc tcatccagat aaatgattaa ggaatcggga
    24001 attgcaatat cttcagctga cgctggaata gttgagctaa attagtcatc atatctttat
    24061 tataaaataa ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta atgtatcatt
    24121 aataaatgtt gaaaaattca gcaactcatt actgtatgat tttagcagcc caaccaacat
    24181 ataccctata aaagctaata agatggttag gaatccttta gaatttttac tcattcatag
    24241 acacttcctt aaataacttt tattatagaa ggtattgtaa ataataatat tgacaaaaag
    24301 tgactcaaaa taaaaaaaaa cttacattat actggaatga actgtattga tagtgtacct
    24361 aaaagctcta ctgacataag atcgtgtaat cggtaaagta attttattaa ttatacggga
    24421 tgtaatcaat tagagaataa actaatcata aaaggtactt ttacattcat aattgtcgct
    24481 ttgagattct actttggttc actatctgac ccaattgcac attttcgatc attacaagta
    24541 ttggtgtcgt tagaacaact aaataaaaac tagcggtaga tttttcagaa tctatgctaa
    24601 tttctgtatg gatgttccag tgatggcaaa tttctctttt cattgttttt cttgtcgcat
    24661 gatatgcctt tttgtgttat tcagttaaac tatagaaatt ctttcctaga cagatcttga
    24721 tcccaaattt tacgtttttg agcatcgata tagtttaaaa tgacattact catatctaaa
    24781 gagtttagat tttttaggac taaaactgtc agttgttctt tttcttcttt ctgttaaagt
    24841 attgtcgcga cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca
    24901 aaaaaacgaa tgacttgagg aaatcacctc aatcattcgt ttaaaatgct atatattctt
    24961 tagtttaaga tacggaaatt aatgccatcg accacactag tagaatgaac gacatttact
    25021 tggccttttt ttaatttcgt tatgtttagc atataaaaat attatagagt ttacttacaa
    25081 tgcttaaaac atatactgta taatctgatc aattttagcc atgtcttgca atggtaagtt
    25141 ttctattttt ttgagttttc gttccttaaa atcaagagat tttaattgtg taataattac
    25201 ttgtcctttt atatctagct cttcaggtaa tgagtaacgt gtcggtaaat tctttatagt
    25261 ggaagtaatt ggacatatga ccgaaaacat tgttttacgg ttaaattcat aacgagagac
    25321 gactagtcct ggtcgtcttt tttgaatttc ttttcctacg gagggatcaa aatcaatcca
    25381 tacaatatca ccttttttag gaatataaat tctttcttct gacactataa aatttctctc
    25441 ccttcaggag ttaaatcttc ccattcgtct ttctcataat actcggcctc ttccccacca
    25501 ctaaatggat cttcaatttt tggaactaac gtaattgttc catcatcaga atacacaacg
    25561 atatattctt ggttttctga aggtttcttt ccgttatcag aaggtaaagt taccactaca
    25621 gaacttcctt gtaatctaga ttttgtaaca agcattatca tcactccctt aataaaagta
    25681 tactatataa cactaagtaa cgcaaaattc ccctttgtaa actatctaga aaattgtttt
    25741 gtttaaagta tatcttattg gatataaata gaaataaatt gtagccagtt cattttttct
    25801 ttaactaaaa aaataatcca agaaacattg aacctatgga tccaaaagct acccaaatac
    25861 tatagctaac cccccgctac cttcaatttt gcagataaaa cgctttggga gaaaatttca
    25921 aagacaacag gccatcctat catgcccgat tataaacaaa aagctggcta ttccttttgc
    25981 tctgacaaat ttcaattggg aaagttcacg aaactagcag gtgaagaagt ttcaactgtt
    26041 cggatcaaag aatgcccgct tcaaatagaa acattggttc aacgaatttc aactagacaa
    26101 gactttgcta ttgtagaacg tgagatcgta gctatttttg tagaagaagg gattttacat
    26161 gatccaactc atatcaatgt ggataaatgg caacctctta tctataaatt cagagaatat
    26221 acaactgcca accagtcgtt aactttgaat tttagattcc aagaattcaa agagtcataa
    26281 tgataaacat cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa aacaaataga
    26341 agctatacat tagaaaggtc gactgtgacc ctatagatgc agtatatgca gcttatgagc
    26401 atttcttctg ttctctttgt ttttgggatc tgtgctgaag tctttttcgt tttttgccgc
    26461 tctttctttg aaaaatcgat tatgttatac cttctccatt actaattcag gcatctgctg
    26521 agtaatgatt tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt gttcttcagt
    26581 caagacaagc ggcattcggt catggattgg tgcgactgtg cggttaggtt cagtagtcat
    26641 tatgattgat tctgccattt tttcatcctg atggctacga taaaaaccgc caatatacac
    26701 tattttttcc ttatttgaaa acataaattt gtctttttct tcattccact cattgggaaa
    26761 acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac tgtctctgca
    26821 cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt catgatttcc
    26881 ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga cggaaaaact
    26941 tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc tcgataataa
    27001 ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct aatccctccc
    27061 aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac gctttcaatt
    27121 tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg tattctcctg
    27181 ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct ttttgaaatt
    27241 gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac ggtgttcaaa
    27301 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagagagcta ttttcacaaa
    27361 atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc
    27421 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca gctattttta
    27481 tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc actaaacgat
    27541 gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    27601 gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct aatacacggt
    27661 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt gagaagcaat
    27721 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctaattatt
    27781 aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta
    27841 gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca gcaatcaacg
    27901 ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt gccaggatat
    27961 tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga cggtattctt
    28021 catccactgt actcaactca ataatcaaat tacctacagg acagccatga taagtgatct
    28081 gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct tctggctgtt
    28141 ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga
    28201 tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga ccttttcctg
    28261 tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt ttataaataa
    28321 gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc ctttttcatt
    28381 tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc gtttaaaaaa
    28441 gactgtgcca aattatctga cacagccctc actataaaac tgttctcata tagctagatg
    28501 ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata gttgctctaa
    28561 tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca acgctgcatc
    28621 cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat tgaatccact
    28681 ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc caccaagaat
    28741 tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa
    28801 gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca attcttcgat
    28861 aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag caaacatctg
    28921 ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt catgtgggta
    28981 aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt ttaccatttc
    29041 aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac caaagccacc
    29101 gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg tatttttatt
    29161 attgaacatg gtggattctc cattcgttat ttattttaat acatcattta gagcttcact
    29221 cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt ttccattcat
    29281 agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac ttgcccctag
    29341 aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat cttctaatac
    29401 ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga cgccttgtgc
    29461 tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa atacactgta
    29521 aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact gatccataat
    29581 gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga cgtctccaat
    29641 cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg ctccgcgatc
    29701 tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt ttcttccagt
    29761 tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg taaaggtgac
    29821 ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat ggaattcgat
    29881 tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag
    29941 aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga acattgatgc
    30001 aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta attgttcctg
    30061 atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt tgattggtaa
    30121 aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa tcactttttc
    30181 accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac
    30241 tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa
    30301 tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa
    30361 tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac gtgttgactt
    30421 ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag tcttaccgcc
    30481 ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct cgcttctttt
    30541 ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc caaatcatct
    30601 gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt agtctggttc
    30661 gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct ctagttgctt
    30721 tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga
    30781 tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca tctaaattat
    30841 aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct ttgatcatat
    30901 gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt tctggatttg
    30961 ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca tttggtgcct
    31021 cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct tttttcattg
    31081 tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta tgacaagtaa
    31141 ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc tactattttt
    31201 ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc aaaataaata
    31261 gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata taccagaaag
    31321 ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat tttttcctcc
    31381 ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg ccgtccaagc
    31441 aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat cagaaaaatg
    31501 tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt catcattttc
    31561 tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc
    31621 gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga agttcatccc
    31681 tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg
    31741 ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt tcaactcttt
    31801 gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca tcaggatctt
    31861 tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag cgtgtccgct
    31921 tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg ctttattgat
    31981 tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt caatgaatct
    32041 ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat gctcttttaa
    32101 tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc cagaagcact
    32161 gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg cttcatttgc
    32221 taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg ggatatgatt
    32281 aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt
    32341 caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa tgattttcca
    32401 accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg tgactaaatc
    32461 ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt cgctttttat
    32521 tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc cttctgaggt
    32581 aaagaatttt attccattat aataataggg aagatggcta gcagtcagca taaccgcagc
    32641 atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag tagctaattc
    32701 gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct ctttcagtgc
    32761 tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat ttggatcggt
    32821 tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt ttaccatgtc
    32881 tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa tatccgatcc
    32941 attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc cctcattttt
    33001 tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat aatgaatgta
    33061 tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag ccaattcgtt
    33121 accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac agactggata
    33181 cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga aacagatggc
    33241 tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac taacttcatt
    33301 tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc
    33361 gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg gatcagtgct
    33421 tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca aaagataagt
    33481 aatatttctt catattttgg tattttttac ttttattttt tgttttcatt caggttgcta
    33541 acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg attttcttgg
    33601 atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt cttttgcgat
    33661 gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga cgtgtaagat
    33721 ttgggcacaa agaccttctt ccatcttttt gaatactact tgttctttca agtcttccgg
    33781 aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa agtcaggctg
    33841 tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat gcaaatactt
    33901 ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat aatcaggaat
    33961 cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata gacaggctac
    34021 tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa agaatgattt
    34081 tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt ctttacgcca
    34141 ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta ttatcatacc
    34201 tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg ctagaataag
    34261 tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag gcggtttcta
    34321 tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga tgtaaatgac
    34381 aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga tgtcttttgc
    34441 gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca gacagtttga
    34501 tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga tttcgatcat
    34561 tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc
    34621 aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct tcatggtcct
    34681 attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt tgatgttcca
    34741 tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt
    34801 gacaacggcc attgctccag caattggccc cacttatgga ggcattatga cttcacattt
    34861 tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt tgattatcgg
    34921 ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt ggataagcgt
    34981 tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt tgggaacagt
    35041 ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt actatcgaag
    35101 taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa cattccgctt
    35161 gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt ttgtcttgcc
    35221 gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag cgatgcttcc
    35281 cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg atcaatatgg
    35341 tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc tagcattatt
    35401 cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt tttatatgat
    35461 cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac tgaatgaaga
    35521 actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg gcgctgtggc
    35581 tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc ataactatga
    35641 agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt
    35701 aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga ttccatatac
    35761 ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt
    35821 tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgaccct agccagttac
    35881 ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca ggcaactaaa
    35941 gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca atggtatcgt
    36001 ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca acttgatcaa
    36061 acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt agctgaattg
    36121 gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt
    36181 tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc actgcgtatc
    36241 agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca gcgtttagca
    36301 caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag cgattcttgg
    36361 ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac acctggcccg
    36421 gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc gcaaaaaggg
    36481 cagctcttag catttcagac atcttataag atgagtcaag actggcctgt tgctatgctt
    36541 gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg tgctacccat
    36601 gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg attgactaag
    36661 agtacagaac agttcttgac tgatccagat cagataaaaa actggccata cgagttccgt
    36721 gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact gccagatgat
    36781 ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg tccttatatc
    36841 ggctatctat tagccaactt cttaaatact ggtcattggc ctacggaaga ttatcaaaag
    36901 gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt caagtaagga
    36961 tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta gttcaatttc
    37021 tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt tcctatctga
    37081 gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt ataggacgta
    37141 tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc aaatggacca
    37201 aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa tgtcgtttca
    37261 ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc tgtttgctgt
    37321 ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac acggaaagga
    37381 ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat attttccgcg
    37441 tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg gtcattgatc
    37501 ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc gatcttttcg
    37561 ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg cccatttccg
    37621 tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt cacgcccaca
    37681 ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc ttgtgtttta
    37741 tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat aacgaaagca
    37801 tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt aatcgttgat
    37861 tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt actagcatat
    37921 tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc
    37981 gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc cgctgtatta
    38041 tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca ttcgggatca
    38101 caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt ctctaagatc
    38161 ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc aaatccttct
    38221 ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat tacttttgta
    38281 tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt ctcgtatttg
    38341 ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct tcccacaatg
    38401 cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc ttcagcatgt
    38461 gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg ataagctgtc
    38521 ttcctatcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac aatcgatcga
    38581 atactggata acagtggatt tccacttgtg atttcgttta attcgagact ctcttttgta
    38641 ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata cattattcga
    38701 gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac
    38761 cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg gcttagtacc
    38821 agccgtttgc taaccagaat gttttagcag catcccatga accataacga gatgttacat
    38881 attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag tatgaagcat
    38941 ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg cctgattctt
    39001 tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca gctggggcag
    39061 caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc gttgctgttt
    39121 cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa gaatcatttg
    39181 aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt
    39241 tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag atcttagata
    39301 atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta gttcctacaa
    39361 acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta aatgaattca
    39421 tattaaactc ctttttttct c
//
LOCUS       DAZUMV010000014        37823 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000014 DAZUMV010000000
VERSION     DAZUMV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.014"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            178..408
                     /locus_tag="ACG8FK_000638"
     CDS             178..408
                     /locus_tag="ACG8FK_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS5096272.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            505..1806
                     /gene="murA"
                     /locus_tag="ACG8FK_000639"
     CDS             505..1806
                     /gene="murA"
                     /locus_tag="ACG8FK_000639"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5096273.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1819..1995
                     /locus_tag="ACG8FK_000640"
     CDS             1819..1995
                     /locus_tag="ACG8FK_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5096274.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2130..2426
                     /gene="yidD"
                     /locus_tag="ACG8FK_000641"
     CDS             2130..2426
                     /gene="yidD"
                     /locus_tag="ACG8FK_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS5096275.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2611..3078
                     /locus_tag="ACG8FK_000642"
     CDS             2611..3078
                     /locus_tag="ACG8FK_000642"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5096276.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3163..4047
                     /locus_tag="ACG8FK_000643"
     CDS             3163..4047
                     /locus_tag="ACG8FK_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5096277.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4161..4397
                     /locus_tag="ACG8FK_000644"
     CDS             4161..4397
                     /locus_tag="ACG8FK_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS5096278.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4485..4565
                     /locus_tag="ACG8FK_000645"
     tRNA            4485..4565
                     /locus_tag="ACG8FK_000645"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4519..4521,aa:Tyr,seq:gta)
     gene            4572..4643
                     /locus_tag="ACG8FK_000646"
     tRNA            4572..4643
                     /locus_tag="ACG8FK_000646"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4604..4606,aa:Gln,seq:ttg)
     gene            4768..4938
                     /locus_tag="ACG8FK_000647"
     CDS             4768..4938
                     /locus_tag="ACG8FK_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096279.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5158..5865
                     /locus_tag="ACG8FK_000648"
     CDS             5158..5865
                     /locus_tag="ACG8FK_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5096280.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5924..6568)
                     /locus_tag="ACG8FK_000649"
     CDS             complement(5924..6568)
                     /locus_tag="ACG8FK_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS5096281.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6694..6981
                     /locus_tag="ACG8FK_000650"
     CDS             6694..6981
                     /locus_tag="ACG8FK_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096282.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7089..7409
                     /locus_tag="ACG8FK_000651"
     CDS             7089..7409
                     /locus_tag="ACG8FK_000651"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5096283.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7490..8803
                     /locus_tag="ACG8FK_000652"
     CDS             7490..8803
                     /locus_tag="ACG8FK_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5096284.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8800..9519
                     /locus_tag="ACG8FK_000653"
     CDS             8800..9519
                     /locus_tag="ACG8FK_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS5096285.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9647..10198
                     /gene="raiA"
                     /locus_tag="ACG8FK_000654"
     CDS             9647..10198
                     /gene="raiA"
                     /locus_tag="ACG8FK_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS5096286.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10420..12954
                     /gene="secA"
                     /locus_tag="ACG8FK_000655"
     CDS             10420..12954
                     /gene="secA"
                     /locus_tag="ACG8FK_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS5096287.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13080..14181
                     /gene="prfB"
                     /locus_tag="ACG8FK_000656"
     CDS             join(13080..13151,13153..14181)
                     /gene="prfB"
                     /locus_tag="ACG8FK_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS5096288.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14286..14972
                     /gene="ftsE"
                     /locus_tag="ACG8FK_000657"
     CDS             14286..14972
                     /gene="ftsE"
                     /locus_tag="ACG8FK_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS5096289.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14965..15849
                     /gene="ftsX"
                     /locus_tag="ACG8FK_000658"
     CDS             14965..15849
                     /gene="ftsX"
                     /locus_tag="ACG8FK_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS5096290.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRHREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16001..17449
                     /gene="cls"
                     /locus_tag="ACG8FK_000659"
     CDS             16001..17449
                     /gene="cls"
                     /locus_tag="ACG8FK_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5096291.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17786..18643
                     /locus_tag="ACG8FK_000660"
     CDS             17786..18643
                     /locus_tag="ACG8FK_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS5096292.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18758..19678
                     /gene="pstC"
                     /locus_tag="ACG8FK_000661"
     CDS             18758..19678
                     /gene="pstC"
                     /locus_tag="ACG8FK_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS5096293.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19678..20562
                     /gene="pstA"
                     /locus_tag="ACG8FK_000662"
     CDS             19678..20562
                     /gene="pstA"
                     /locus_tag="ACG8FK_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS5096294.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20577..21383
                     /gene="pstB"
                     /locus_tag="ACG8FK_000663"
     CDS             20577..21383
                     /gene="pstB"
                     /locus_tag="ACG8FK_000663"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5096295.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21399..22157
                     /gene="pstB"
                     /locus_tag="ACG8FK_000664"
     CDS             21399..22157
                     /gene="pstB"
                     /locus_tag="ACG8FK_000664"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5096296.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22170..22847
                     /gene="phoU"
                     /locus_tag="ACG8FK_000665"
     CDS             22170..22847
                     /gene="phoU"
                     /locus_tag="ACG8FK_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS5096297.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23423..24115
                     /locus_tag="ACG8FK_000666"
     CDS             23423..24115
                     /locus_tag="ACG8FK_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5096298.1"
                     /translation="MLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24112..24879
                     /locus_tag="ACG8FK_000667"
     CDS             24112..24879
                     /locus_tag="ACG8FK_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5096299.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24979..26547
                     /gene="liaX"
                     /locus_tag="ACG8FK_000668"
     CDS             24979..26547
                     /gene="liaX"
                     /locus_tag="ACG8FK_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS5096300.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26844..27164
                     /locus_tag="ACG8FK_000669"
     CDS             26844..27164
                     /locus_tag="ACG8FK_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS5096301.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27164..27514
                     /locus_tag="ACG8FK_000670"
     CDS             27164..27514
                     /locus_tag="ACG8FK_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS5096302.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27681..28619
                     /gene="hprK"
                     /locus_tag="ACG8FK_000671"
     CDS             27681..28619
                     /gene="hprK"
                     /locus_tag="ACG8FK_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS5096303.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28634..29464
                     /gene="lgt"
                     /locus_tag="ACG8FK_000672"
     CDS             28634..29464
                     /gene="lgt"
                     /locus_tag="ACG8FK_000672"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS5096304.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29508..30533
                     /locus_tag="ACG8FK_000673"
     CDS             29508..30533
                     /locus_tag="ACG8FK_000673"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5096305.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30548..31486
                     /gene="galU"
                     /locus_tag="ACG8FK_000674"
     CDS             30548..31486
                     /gene="galU"
                     /locus_tag="ACG8FK_000674"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5096306.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31611..32051
                     /locus_tag="ACG8FK_000675"
     CDS             31611..32051
                     /locus_tag="ACG8FK_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS5096307.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32054..32656
                     /locus_tag="ACG8FK_000676"
     CDS             32054..32656
                     /locus_tag="ACG8FK_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5096308.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32715..33821)
                     /locus_tag="ACG8FK_000677"
     CDS             complement(32715..33821)
                     /locus_tag="ACG8FK_000677"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS5096309.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34031..35032
                     /gene="ccpA"
                     /locus_tag="ACG8FK_000678"
     CDS             34031..35032
                     /gene="ccpA"
                     /locus_tag="ACG8FK_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS5096310.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35369..37714)
                     /locus_tag="ACG8FK_000679"
     CDS             complement(35369..37714)
                     /locus_tag="ACG8FK_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS5096311.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT        12317 a         6545 c         8118 g        10843 t
ORIGIN      
        1 gggactgcga caagactttt gtctcagtcc ctttttttgc atatttttag catttatttc
       61 ctaaaaaatg taaggggatt cctatatttt taagtatact tgtttttcct tttatacatt
      121 ttatggtatt atcattttat tagttattat aaaacaatcg ttagaaagga cgtcattatg
      181 caagtatacg gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt tatctactta
      241 gccttctggt ctttacgttc gttacgcatt gaaacttttt tcagaccaat gcataatacg
      301 caaattagaa tggcaatcgt acttttttca atttttttag ggtacactgc aagtaatttt
      361 ttcttagaag ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat tttaaaaaaa
      421 ggtactattt taaaagcaag gatgttataa tagaaaagat tttgagtaag tgtatgctca
      481 ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg caatcaatta
      541 aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt agctgctagt
      601 ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc tgatgtcttc
      661 actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga acaaaagaat
      721 caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga gtatgttagt
      781 caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg acatgcaaaa
      841 gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca tttaaaagga
      901 tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc aattgcggat
      961 gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac tcaaaatatc
     1021 atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc tcgcgaacca
     1081 gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta tggagctggt
     1141 actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca ctcaatcgta
     1201 caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca aggcaatgtg
     1261 ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt gatcgaaatg
     1321 ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa acatatctta
     1381 cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca agctcaaatg
     1441 acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt gtttgaaaat
     1501 cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat cgacggaaat
     1561 gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc aacagacttg
     1621 cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac acgtgtgcgt
     1681 aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca gcaattaggt
     1741 gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc acaaacgatt
     1801 gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt gataaagatt
     1861 ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat cggatatggt
     1921 gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat tcatattcga
     1981 gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa gtgagccatg
     2041 aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg atcccttttt
     2101 ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat ttagttagag
     2161 gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac ccaacctgtt
     2221 ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg gtaatgggga
     2281 ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat gtaccattga
     2341 cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa aaggttgtga
     2401 aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc ttatattttg
     2461 caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa aaaggttgtg
     2521 aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc aaagtaacaa
     2581 ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa gaaagattta
     2641 ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt atatgtttcc
     2701 agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact gatctatgtg
     2761 gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta tcaaggaaag
     2821 gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact cgcttccagt
     2881 caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca tctctatcaa
     2941 aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca ttactctgag
     3001 cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat tcacttacct
     3061 aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat gctaaataga
     3121 tacattacaa tagctttagc aaacaaaaag gagaatattt ttatgggaat attagttttt
     3181 gattttggtg gatcggctgt caaatatggt tgttgggatg gcaaagagat taaaggtaag
     3241 ggacaatttg caacgcctga atcttgggaa gaaatgaaag cgcaattatt tcaagtttat
     3301 aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc tggagtagtg
     3361 aataatgaga aacaggtgat tgagggaatc agtgcgattc cttatattca tggatttaat
     3421 attttccgag atttggaaac tctgtttcaa cttccagtaa cgattgaaaa tgacgccaac
     3481 tgtgccggaa tggctgaatt ttatgaaggt gctgcccgcg attatcaaga tgttgctttt
     3541 gttgtggcag gaactgggat tggcggggcg ttgttcacga aaggacaaat caataaaggg
     3601 gcacatcttt acggtggaga atttgggttg atgttcttaa atggcgataa gacattcagt
     3661 aaacttggta cagcggtaca gatggcttgg cgttattgtg aacgcaaagg tctcgataaa
     3721 aatgcttata ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc aatagcaaaa
     3781 gaagaagttg agagttttta tacgtatctt acaaagggat tattcagtat tcaattttcg
     3841 tttgatccag aagtgatcgt attaggcggc ggggtttctg caaaagaagg gctcattgat
     3901 gagatcaaaa gccgaatgaa gaagctgaca gaacaattcg atctgcatga tttcgaaccg
     3961 cagattttat tatgtgaata cagaaacgat gctaatcttg taggtgcagc agcaaactat
     4021 ctcgctgtac gtcaagggga gaattaagag ctttagagaa ggtgcagctt gcataaaaga
     4081 agtgaatgaa atgaatagga gatttttacc taaggaatgt tttatttgct gcagtatgat
     4141 aaaatatctg agaggtgaaa atgatgtcaa aaaaaaataa agatattgaa gtgcgtatag
     4201 aagaagtgaa aaaagatatc aaaggtaaga attatgaagt cactcagtta tttataggga
     4261 aaaaaatgat tggtgagatc ttagcctatg gaccaaaaga atatgaaatt ttctttggtg
     4321 aagaagattt tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg attcgacact
     4381 ggaatctgca cgaataaaaa aatatcaaaa aaactttcaa taaagggttg caaaaatctg
     4441 gaaaattagg tattataact aagtcaggtt gatacaaacc tttcggagga gtagcgaagt
     4501 ggctaaacgc gacggactgt aaatccgttc cttagggttc agtggttcga atccactctc
     4561 ctccatttca ttggggtata gccaagcggt aaggcaaggg actttgactc cctcatgcgt
     4621 tggttcgaat ccagctaccc cagtatacca caatcgtaag ctctcgttag ttcgagagct
     4681 cttttttttg ctttgctacc ttgttccttg aagattctgc tgatttaaga tagagttaag
     4741 ttaactacaa gaattggagg gatccacatg aaatggaaag aaaaagtaga cgaagcagct
     4801 gaaaagttat atgatttggt caaatcagaa aaatacaata ttgaagtgaa tattccgaaa
     4861 aaaggcggaa aagcggtccg ggtaaaatca aaacgcccaa cgaatcatac aaaaaaatgg
     4921 atggctaaaa accgctaata ataacaaaag atccataagt cggcattttc atcgatttta
     4981 gggtcttttg ttattttcta ggttggacat atctaaaaaa ttgtttctga caataggagt
     5041 aggaagaacg atgtgtgtct aaatcctctg ttgatggata gaaaccttta tctttttcag
     5101 ttgtgaatca ggtatatacc agttataata gaagaaacga aaggacgggt gtacgttatg
     5161 tcgaatcaat tacctgtata tatacagatt catgaccaat taaaaaatga aatcgaacaa
     5221 ggtatatgga aagtcggaga ccgtcttcct tcagaaagag aattagcagt gaaattcaat
     5281 gtcagccgta tgacgttgag acaagcaatt caaaacttgg cagatgaagg aatcttagaa
     5341 agaaagatcg gttcgggtac atatgttgca cgagaaaaag tacaagaaaa aatgtctggt
     5401 gcaacaagtt ttacagaaat tatggaatca caaaatcgag taccgtctag tcggacaatc
     5461 tcttatttct tgacttcgcc aagctcaagt gaaatggaaa aactaaactt gagtaaagat
     5521 gacacgatct tgcgaatgga acgtattcgt ttcgcagatg atgtccctat ttgttttgaa
     5581 gttgccagtg tgccacaaaa gctgatcgat ggctacagca aagcagagat tactgaatcg
     5641 ttgtatcgca cgctagaaga aaaaggcggg aaaaagatcg gtccagcgaa tcaaacgatt
     5701 actgctgtgt tggcttctga gaaaattgct gactatttag aggtaaaacg aggagatgca
     5761 gttcttcgtt tgcgccagat ttcttatttt gaagacggta cgccgtttga gtatgtgcgt
     5821 acgcaatacg taggaaatcg ttttgagttt tatttggaaa aataaaaaac aggtgatccc
     5881 tgattccgta ttagaaataa aagagaatca gaatcacctg tttttatata tgatcagtta
     5941 ccagttgctc gtgataagaa acaggaccct cttcaaattg gacttgtccg ttcagcagct
     6001 caatgatgga ttctttaaag tggtcagcac ttttttcatc tacagcacag atgacgctaa
     6061 cttgatcagt atattggatt tcttttataa aaaaatgttg gagttcaaga taattctgta
     6121 attttcctag ttgcggatac gtgatcatca cagagatctc ctgttgcaag gtaccttcta
     6181 cgattccaat gtcagacaat gcttgagaga ccgcatgagt gtatgctcgt attaatccgc
     6241 ctgtgccgag tttcgttcct ccaaaatatc gagtgactac tgcagctaca ttgatcagtt
     6301 gatttttttt gaggacttcc aacatcggta tgcccgccgt tccacttggt tctccatcat
     6361 cgctgcttcg ctggatttca tttttttctc caatgatgaa ggcgctgcag ttatggttcg
     6421 ctttccaatg ttcttttttt atttcttgaa tgaaactttt tgcttcgtcc tctgattcta
     6481 ttcgtctaag agaacagata aagcgtgatt ttttgatctc gatctcactt tgtccatttt
     6541 cttttattgt acgatagcgc tcgatcatct tgtcacctct tttttcattt tctttattca
     6601 gtataaataa agttctaggt tgtttcaaca ctgtaacggt tgcgcaattg ctgtagtatg
     6661 cgataataaa gacaaaggca ggtggatctg gggatgcagg aacgaatgaa aaagtacgat
     6721 gcaattacgc attatttgaa gaacaatggc ggctctcaag taacgttgac ctttacccaa
     6781 tttgacgaac tgctgtttcc cagtaacgga cttcccaaaa cagcaaggga aagtacagat
     6841 tggtgggcaa atgattataa acaccctgaa aaaggtgcct atggctggat aaatgcaggc
     6901 tatgaagtag ttgtcatcaa tttggacaaa gaatatgtcg tgttcaataa attagtgaaa
     6961 tctagttggc tatttgatta aataaaccgc gtttagaaaa cgttgacaaa tttctttctt
     7021 tttatataat agagtaacag acgcgaagaa aacgtttgcg tagtttgttt taaaaaggag
     7081 agaaaagtat ggctaaaaaa acaatcatgc ttgtatgttc ggctggtatg agcacaagtt
     7141 tactagtaac aaaaatgcaa aaagctgctg aagcaaaagg aatcgactct gatattttcg
     7201 ctgtgtccgc ttctgacgca gataacaatc ttgcaaataa agacgtggat gtgttgttat
     7261 taggcccaca agttcgcttt atgaaagctg attttgagaa acgtctggaa ccaaaaggca
     7321 ttcctttaga tgtcatcaat atggctgatt atggtatgat gaatggcgaa aaagtgttag
     7381 atcaagcaat cacgctcata ggtgaataaa tagaacagta taactatcga acaagcgatc
     7441 caaaaaatag atcgcttgtt ttttcgtata tttttttagg gcgaataata tgaatcattt
     7501 ttggggtaga aggcttctga cgagagatat taaagcaata gactgtgagg aggtaaattt
     7561 accaaattat gagaagatcg cagcagtgga acgtttgagc aactggattc tttgtcatcg
     7621 ctgtggaatg agaacgtccg tcattgatgg acagcttgca gattacggtt atttttgtac
     7681 acattgtcta tatttagggc gatgcgacac tcaacaagat ttatatttgt ttgatcagcc
     7741 tgaagcaaaa cctcgagaag tcgtcttttt ttggacagga cagcttactg aaaaacaaac
     7801 ggaaatctca aagagaatac tttgccattc agaaaaaaga catcatctta tttgggctgt
     7861 aacaggtgca gggaaaacag agatgcttta tcctatttta gtggaaactt taaaagctgg
     7921 aggaagagta gcgatttgta caccgcgagt cgatgtttgt aatgaattgt ttttacgcta
     7981 tcgttatgtt tttccagaag aaaaaatcac gttgctccat gggaatacag caacggctta
     8041 tacctttact tcttttgtta tctgtacgat tcatcagcta taccgttttt atcggtcctt
     8101 tgatttaata gtgatcgatg agatcgatgc gtttccttat aacggggatg caggattagc
     8161 tcatgctgtg caaacagcga ttaaaccaga cggacgattc atctacttaa gtgccacacc
     8221 tgataaaaag ttactaaagg agatcaagca gacgtttgac atccacaaac ttgctttacg
     8281 ctttcatcgg cgtttgcttc ctgaacccgt cttggttttc atgaataatt ggcgacaaaa
     8341 atgcaagaac aagaagaaga taaaatcttt ggtgcggatc attcatcaat taattaaaga
     8401 aaaccatatc cttttatttt gtccaagtat cacgatgatg catcgtttga aagccacact
     8461 tcaagaggct cttccgagat atgagatcac agatgtttct tcaagagaca atcagcgggc
     8521 ggaaaaagtc cagaaaatgc gagaagcaaa atatgacata ctgttaacca cgatgatcct
     8581 tgaacgtgga gtcacatttg aaagaatatc cgttatcgtt ttaggagcgg atcatccggt
     8641 ttttactaaa tcttctttgg tacaaatcgc aggaagagcc gatcgtaaag ggccttatac
     8701 gaacagtcaa gtatattttt tctatgaaga aaaaactgca gctattcgaa aagcttgtca
     8761 ggaaatcaaa gaaatgaatg aagaagggaa gaagatactg tgaggtgcag ttgctgcagt
     8821 aaagatatcg tccaaaattt aacattcagc gagattattg gtgtttcgaa atacagatta
     8881 cctcagactt gttcagtttg tcgcgcacaa tttcatttat tggaaacaag aggctgttct
     8941 ggctgtcaaa aaccaatcca tttcaatcta gagagtacga aaaagaaagt aaattctatc
     9001 cccgtttact gcagtgactg tcaagaatgg aagaagaaat atccgaccta tgattttcag
     9061 caccgtgcct tttttgtgta cgatgaaaat atgaaggcct ggctcaatca atacaagttt
     9121 atgggagata tccgactagc aggcaccttt gcatataaat ggacgcagat aaaaaaagaa
     9181 tacccgacat acatctattg tccaatacct ttatctgaaa aaaggcgaac agaaagagga
     9241 ttcaatcaag taagcgagat gttgaaagca gcaagaattc cttttcagat gctactcaaa
     9301 agagagaagc atctgctggc tcaagcaaaa aacacgcgaa aggaaagatt ggagatgcct
     9361 caaccttttg aacttgcagt caccttttct aagatacaaa ataaaaaaat attgattatt
     9421 gatgatgtct atacaacagg acagactctt ttccacgcag cagattgttt gcagatcgca
     9481 cagccgaaaa tcatccgaac tatttctttg gcgagatgaa ttagatgtgg aaatttgata
     9541 gcgatttaat ttgcaaataa cctagagttc gatataatag aattaagaag agggaaagag
     9601 tggtccttct ccttcttgat tgtggtagac gaaaggggta atttttatgt ttagatataa
     9661 tgtacgcggc gaaaatatcg aagtaactga agcgattcgc gactacgttg agaaaaaagt
     9721 aggcaaacta gaacgctatt tcagtgacgc acccgatgca acggcgcatg tgaacttaaa
     9781 agtttataca gaaaaaacag caaaagtgga agtaacgatc ccattacctt atcttgtatt
     9841 acgtgccgaa gaaacttctc cagatttata cgcaagtatc gatttggttg ttgacaaatt
     9901 ggaacgccaa atccgcaaat tcaaaacaaa aattaatcgt aaatcccgtg aaacgggcat
     9961 gaatactgca aatgctgctg ctttattcaa tgaagaggac gaaaacgaaa atgattctga
    10021 attagatatc gtacgtacga aacgcctttc attgaaacca atggatagtg aagaagcagt
    10081 cttgcaaatg aacatgctgg gacataactt ctttattttt gaagatgcag aaacaaatgg
    10141 tacaagtatc gtttaccgcc gtaaagatgg aaaatacgga ctgattgaaa cagactgatc
    10201 agccttactt aaataacaac gaaaacttgc agcagctact gacagctgcc gcaagttttt
    10261 ttgagtttcc tcctctttta gatgaaatat gaataaatta tgaagttctt actttaatac
    10321 gcttaagata tttcaattat ctataactaa tgataaaatg aaaagtaaag tctacatagt
    10381 ctggataata cacagaagga aaaggagatt acacaacgca tggccaattt gattcgttca
    10441 cttatcgaaa acgataagaa agaactaaaa cgtttagata gcattgcaaa aaaagttgaa
    10501 gcatacgctg accaaatggc agcattaact gatgaacaat tacaggcaaa aactgctgaa
    10561 ttcaagggca gataccaaaa aggagaaaca ttagatcaat tacttccgga agcttttgca
    10621 gtcgtccgag aagcagcaaa acgtgtatta ggtttatatc cataccacgt acaattgatg
    10681 ggtggaatcg tgcttcacga tggaaatatc cctgaaatgc gtactggtga aggtaaaacc
    10741 ttgaccgcaa cgatgccagt atatctaaat gctctgactg gcgaaggggt acacgtagtt
    10801 accgtcaatg aatatttagc aacccgtgac tcgacggaaa tgggcgaact ttataatttc
    10861 ttaggattaa cagtggggtt gaatatcaac tcaaaatctt cggatgaaaa acgcgaggct
    10921 tataattgtg atatcactta tagtacgaat aatgaattag gatttgacta cttgagagac
    10981 aatatggtcg tttaccgcca tcaaatggta caacgtccgc tgaattatgc cattgtcgat
    11041 gaagttgact caatcttgat tgatgaagcg agaacgccgc tgatcatttc agggcaagca
    11101 gaaaaatcca cagctttata tacaagagca gacaacttcg tgaaacgttt gaaagaagaa
    11161 gaagattata aaatcgatgt acaatcaaaa acaatcagtt taaccgaagc agggatcgaa
    11221 aaagcagaag aaacattcgg tttggataac ttatacgaca ttgaaaatac tgcattgacc
    11281 catcacttgg atcaagcatt gagagccaac tatatcatgc ttttagatat cgattatgtt
    11341 gtccaagacg gaaaagtact gattgttgac cagttcactg gtcggatcat ggatggacgc
    11401 cgttattcag atggacttca ccaagcaatc gaagctaaag aaggcgtaga gatcgaagac
    11461 gaaacgaaaa ccatggcgac gatcactttc cagaactatt tccgtatgta caagaaactt
    11521 gcgggaatga ctggtacagc taaaacagaa gaagaagaat tcagagaaat ttataacatc
    11581 caagtatacc agattccgac aaatcgtccg gttatccgtg atgatcgtcc agatttgctt
    11641 tacccaacat tacaaagcaa gttccacgct gtcgtgcaag atatcaaaga tcgctaccgc
    11701 aaaggtcaac ctgtgttagt cggaactgtt gcggttgaaa catctgagtt attatcagat
    11761 atgctgaatc aagaacgtat tcctcatgaa gtgctgaatg cgaaaaatca ctttaaagaa
    11821 gcagagatca tcatgaatgc tggtcaaaaa ggtgctgtga cgattgctac caacatggct
    11881 ggtcggggaa cagatatcaa gttaggactt ggggtaagag aacttggtgg tctagcagtt
    11941 atcgggacag aacgacatga atctcgtcgt atcgataacc agcttcgagg acgtgcagga
    12001 cgtcaaggag accctggtat gtcacagttc tacttgtcat tggaagatga tttgatgaaa
    12061 cgattcggtt cagaacgaat caaagctttc ttagatcgga tgaagatcgg tgaggaagat
    12121 gctgttatcc aaagcaagat gctgacgaaa caagtagaat ctgcacaaaa acgtgtagaa
    12181 ggaaataact atgacacacg taaaaacgtc ttacaatacg atgatgtgat gcgtgaacaa
    12241 cgtgaagtaa tctataaaca gcgacaagaa gtaatcatgg aagaaaaaag cttatccaag
    12301 ccactaatga acatggtcaa acggacaatc agtcgtgtag tagatgcgca tacacaatta
    12361 gagaaagaaa attggaattt ggaaagtatc atcgattttg ccggcagtac tcttgtccat
    12421 gaagatagta ttactcttgc agatatcgaa ggaaaaacag ctcaggagat caaagagtat
    12481 ctaaacgaac gagcacaaga agtatttaat accaaagcgg ctcaactaaa tggtccagag
    12541 caactgttgg aatttgaaaa agtcgtgatc ttgcgtgttg tggactctaa atggacagac
    12601 catattgacg caatggatca attacgtcag tcaatcggct tacgtgctta tggacagaac
    12661 aatccattag tcgaatatca aacagaaggc tataagatgt ttgaagatat ggtaggcgcc
    12721 atcgagtatg aagtcactcg aatctttatg aaagctgaga tccgtcaaaa cgtacagcgt
    12781 gaacaggttt ctcaaggaac agcttctcac tcagaagacg gagatgccaa agaaaatagt
    12841 aatactagcg cgaaaaaaca gccaatccat gtgaacaagg tcggaagaaa tgatccttgt
    12901 ccttgtggaa gcgggaaaaa atataagaac tgtcatggaa aaaatgcaaa ctaatttctg
    12961 acagcaggat tggtgagaca aattcttttt ttaagaattc gtctcacctt ctttctgtta
    13021 tttgctgatt ctgttagact agatataatg aaaaaagtat agtgaaagaa ggaaaaaaca
    13081 tggaaatatc cgaaattcga aatctcctga cagaaatgca ggaaaaggtc acaagtttca
    13141 ggaggtctct ttgacttaga tcagctggaa gaagacatcg cggaagcaga agcaaaaatg
    13201 gctgaacctg gtttttggga tgacagcgaa gccgcacaaa aagtgatcag tgaaaataac
    13261 gctaataaag aaacctatga ccaattccat tctcttgccg aagaactaga agaaatcgaa
    13321 ttgatggtgg agatgctgca agaagaacct gatgcagcaa tgcaagaaga agcagaaaca
    13381 cgtatcaatg cattagatga aaaaatgaag acgtatgaat tatctatgct gttagacgga
    13441 ccttatgatc gaaacaatgc gatcgtcgaa ttgcatccag gggctggcgg aacggagtcc
    13501 caagactggg gcagcatgct tttacgtatg tatacacgtt gggcagagca gcatggctat
    13561 caagtagaaa cactagatta tcaagcaggt gatgaagcag ggatcaagag tgtgaccctg
    13621 ctgattaaag gacataacgc ttttggttat ctaaaatcag aaaaaggtgt tcatcgcctt
    13681 gtacggattt cgccttttga ctcagcgaaa cgacgtcata cttctttttg ttcggtagat
    13741 gtgatgccag agcttgatga aacgatcgat atagaaatca atccagatga tttgaaaatc
    13801 gatacgtacc gagcaagtgg tgcaggtggt cagcacatca ataaaacaga atctgctgta
    13861 cggatcactc acttgcctac aggaacagta gtagctagtc aggcacaacg ctcacaattg
    13921 aaaaaccgtg agcaagcgat ggggatgtta aaagctaagc tttatcaatt agaagtggaa
    13981 aagaaagaac aagaagcagc tgcacttcga ggagaacaaa aagaaatcgg ctggggttca
    14041 cagatccgtt cttatgtttt ccatccctat tctatggtaa aagaccatcg gacaaacgta
    14101 gagacaggaa atgtccaagc agttatggac ggtgaccttg atttatttat tgacggctat
    14161 ttaaaaatgc atctacaata agaacccatt gttaaattga aataaaattg taagattttg
    14221 caagcatgtt gaaacattgc catgctataa tagctaaaga ctgaaaaaga tatggagaga
    14281 acgccatgat tgaaatgaag gatgtaatga agaagtactc aaatggtacg accgctatcc
    14341 gcaatctctc gattgtcatc gaacaaggag agtttgtcta tgtggttggt ccgtctggag
    14401 ccggtaaatc tacattcatc aaattgatgt atcgagaaga aaaagcttca aaaggaactt
    14461 tgaacgtcgc aggtcatgat ctaatagcaa tcaaaaaccg cgaagttcct tatctacgca
    14521 gagaaatcgg cgtggtgttc caagactata aattattacc aagaaaaaca gtatatgaaa
    14581 atgttgcgta cgctatgcaa gttatcggca gaaaacctcg agatatcaaa aaacgagtaa
    14641 tggaagtgct ggaccttgtt ggtttgaaac acaaagttcg agtctttcct agcgaattat
    14701 ccggtgggga acagcaacgt gtctctattg ctcgggcaat cgtgaatacg cctaaagtac
    14761 ttatcgcgga tgaaccgaca ggaaatctgg atccagaaaa ctcatgggaa atcatgaaat
    14821 tattagatcg gatcaatgcc caaggaacta ctgtcgtgat ggctacacat aacagtacga
    14881 tcgtaaatac gattcggcac cgagtaattg ctatcgaaaa cggccgcatt attcgagatc
    14941 aagcggaagg ggaatatgga tacgatgatt agaacctttt tccgccattt attagaaagt
    15001 atcaagagtt tgaaacgtaa cggctggatg acagttgctt cagtcagtgc ggtaacgatc
    15061 acgttgactt tagtcggtgt attcatggca gttatcatga atgcaactaa attagctcag
    15121 gatatagaag gaaacgttga tgtatctgta ttcgtagata tcggtacaaa agacgaagag
    15181 atgaaaaagc tagaaaaaga attgaatgac ttagatcatg ttaaaaaagt tgaattttca
    15241 agtaaagaac aggaattgaa aaaaatccaa aacgaaatgg gtgattcctg gaaattgttt
    15301 gaaggtgaca ataacccgtt atatgatgtg tatgtagtaa gtgctactga tccttcttat
    15361 acaaaacaag tagcagaaga agcagcagat ttgaagaatg tttctcgttc tgattacggt
    15421 ggagcttcct cagatcggat tttccaaatc gctggtgcag ttcggacatg gggattagtt
    15481 gcagcggcat tgctgctgtt cgtagcgatg tttctcattt cgaacacgat tcggatcacg
    15541 attttgtcta gacaccgtga aattcaaatc atgcgtttag tcggtgcaaa aaatggatat
    15601 attcgctggc cgtttttcct tgaaggtggc tggatcggtc tgatcggtgc gattcttccg
    15661 attttgatca tcacatttgg ttatgcatgg tttttccaat tgatcaatcc atcattatta
    15721 cgttcccatt attcattgat tcatccacaa aatatcgttt ggaaaatcaa tcttttaatg
    15781 gtcggcattg gtgtgatcat tggttcactt ggctcgatca tttctatgag acgtttcttg
    15841 aaattctaat accacaatat aaataaaatg agaaagttgg acagtttcgt tccagctttc
    15901 tttttttatt ggctaggaga atagaaggaa gcggcttttt tccctttact ttaaaaaaca
    15961 acttgggtat tcttaagttg gtagaggaag aggtgaagcc gtgaaaatta tcgttgacaa
    16021 tttttttacg atccttttga ttatgaatat tttgctttcc tttatcatcg tttttcgaga
    16081 aagaaaggaa actgctcaaa catgggcatg gctgctcgtt ttgatgttca ttccagttgt
    16141 aggatttatt ttatatattt ttttaggaag agggatttcc aaagagaaaa tctttgattt
    16201 gaagatccaa gggaaaatcg gtaagaacct ggaaattgaa gaagacaaac aggcattgat
    16261 gcgagggctt tatcctcatc cgcctactgg acaagtagat gtgaaacaat tgatctatat
    16321 gctcacggta tttgaaagta cgctctatac gactaaaaat gaaatcactt tatttacaga
    16381 tggtcgtgaa aaattcgatg cgctgattga agatatcaag caagcagaag accatatcca
    16441 cttccagtat tatatttatc gcagtgatgc attaggtgaa gaagttcgag atgcattgac
    16501 agaggcggca agacgagggg taaaagtcag agtccttctt gatgcatggg gttccacaca
    16561 agtttcttcc agcttttttc agaatttgaa aaaagcaggc ggagaaatcg catttttctt
    16621 tcctctattt gttccgtata ttaatcctcg gatcaattat cgcaatcatc gtaaaatcgt
    16681 agttattgat gggaaaatcg gatatacagg tgggttcaat gttggaaatg aatatctagg
    16741 attggtaaag aaatttggtt attggcgaga caatcatttg cgtatttacg gagaagcggt
    16801 gtatatcttg caaaaccgtt ttttgatgga ctggaattct cagcatacaa aagaagtagg
    16861 ctattctccc aaattttttc cgtccattca ctctactggt gatatagctg ttcaaatcgt
    16921 gacaagcgga ccagataccg aacatgaaca aatcaaaatg acttatttga aaatgatttc
    16981 gttagccaaa cgggagatat tgatccaaac gccttattac ataccggatg gatcgattca
    17041 tgaagcactg aagttggctc ttctttcagg cgtaaaagtt catatccaaa tacctaacaa
    17101 accagatcat cttctcgttt actgggcaac gtattctttc gctgcagagc tgatcgagta
    17161 tggagcaagg atcgaaacat atgagaatgg atttattcat gcaaagacga tgattatcga
    17221 tggtggcatt gtttcagttg gctcagcaaa tatcgatgtt cgttcatttc gcttggactt
    17281 tgaggtcaat acactgattt atgatgaacg aatggcttca cgtgtaagaa aagcattttt
    17341 tgaagactcg aaaatttcta cgcatctcac aaaagaaatg tatgaaaacc gaggaataat
    17401 catcaagatg aaagaaggat tagcaagatt gatttcgccg ctcctttaaa acacaaaaag
    17461 gacatgagaa ccatcaacag atggagatct cgtgtccttt tctaacgggg ccatttctga
    17521 gagtacgtca gaaatggaga aagcgtctcg catgagcgaa attaactttg gggtatctct
    17581 tgttttttgg ggaaagcaag aaataagcta atatatgcta ttaaaagaga cacatttcaa
    17641 agccagtata gcatagaaac ttttaatgag atagtttttt ttacaattat gaggatttat
    17701 tttgttattt aatgattttg attcgttttc atcaagtaat gaaaatgata aaataaacat
    17761 actttaggat taaaggagat ttctaatgaa aaaacttaaa ttattcttac caataatagg
    17821 attagctatt cttttatcag gctgttcaaa gtggattgat cgaggagaat ccattacggc
    17881 tgttggctct tctgctttac agcctttagt agaaacagtt gctgaaactt accagacaga
    17941 aaatcctgga aaattcatca atgtacaagg gggcggaagc ggaactggtt tgtctcaagt
    18001 acagtcagga gcagtcgaca tcggaaactc tgatttgttt gcggaagaaa aatcggggat
    18061 cgatgcctca gcattagtcg atcatcgagt agcagttgtt gggatcactc ctattgtgaa
    18121 caaggatatt ggcgtaaaag acatcagtat ggaaaacctg aaaaaactat ttacaggcga
    18181 gatcaaaaac tggagtgaag tcggtggaaa agatcagcca gtagttatat tgaaccgtgc
    18241 ttcaggaagc ggaacccgta gcacgtttga aaaatgggta ttggacggag aaacagctgt
    18301 tcaggcacaa gaacaagatt caagcggaat ggtgcgccag atcgttgccg acacacccgg
    18361 tgcaatcagc tatgttgctt tttcttatgt ttccgatgat gtagatacgt tgaatgttaa
    18421 cggtgtagaa ccgacagatg aaaatgtgac aacgaacaaa tggacgattt gggcatatga
    18481 gcatatgtac acaagagatc atccaaaaaa actgacaaaa gaatttttag actatatctt
    18541 atctgatgaa gtacaagata acatcgttgg agaactaggt tatatccctg tttctcatat
    18601 gaaagtcgaa cgtgattggc aaggaaatat cgttaaagag taatctttac acaaaattta
    18661 caaacttagc taaactttta catgttatta aaaccaaatt gatgttcaat ttgtacactg
    18721 agtgagtatg ttaaacctga ttaaggggga gtcatctgtg gaagatgtga aaaaagtatt
    18781 attaacaaaa tcaaaacgtt caaaaatgga acagcgtgga aaattcatca gttttttatg
    18841 tatcgcgctg atcgtgttgg ttgtattatc tattttttat tttgtagcta gtaaaggatt
    18901 ggccaccttt tttactgata agatcaatgt atttgatttt ctgtttggta cagattggaa
    18961 tccaagccaa atcggagcaa atgggaaacc aatggtaggg gcattgccta tgatcacagg
    19021 ttcgtttatt gtgacctttt tatctgctat tgttgccaca ccatttgcca ttggtgcagc
    19081 agtattcatg acggagatct ctcctaaaaa cggaacgaag atcttacagc cggtcatcga
    19141 gctgcttgta ggtattcctt ctgtcgttta tggttttatc ggtttatcag ttatcgtgcc
    19201 ttttgtcaga agtatttttg gcgggacagg gtttggtatc ttagctggta catttgtgtt
    19261 gttcgttatg atcttgccga ctgtcacaac gatgactgtt gatgcattaa aggcagtacc
    19321 acggcactat cgggaagctt cattagcgct tggtgccaca agatggcaaa caatctacaa
    19381 agtcgtgcta cgagcagccg tgcccgggat tttaacagca gtcgtttttg gtatggctcg
    19441 tgcgtttggt gaagccttag ctatccaaat ggtcatcggt aatgcagcat tgatgccaac
    19501 aggacttact actcctgctt ctacattgac gtccatctta acaatgggaa tcggaaatac
    19561 aatcatggga acagttgaaa ataatgtgtt atggtcatta gcgctgatct tgctattgat
    19621 gtcattattc tttaatatcg tgatccgtat gattgggaag aaaggagagc tgaaataatg
    19681 aacgcaaaac gctcagataa aattgctaca ggtgttttat atgtagtttc ggggatcatc
    19741 gtattgattt tagcagcttt actactttat atcttagttc ggggaatacc gcacatttca
    19801 tggtccttct tgaccgaacc ttccaaagcc taccaagaag gcggtggaat cgggatccag
    19861 ctatttaact cgatttattt gcttttgatc acgatgatca tcagttttcc tatttcttta
    19921 ggtgcgggta tctatctttc agaatatgct ggaaaaaact ggctgacaga tgtgattcgc
    19981 acgtcgattg aaatcttgag ctcattgcct tctgtcgttg tcggtttatt cggtttcttg
    20041 gttttcgtta ttcaattcca gtatggtttc tcgattattt ctggtgcatt ggctttgact
    20101 gtattcaact tgcctctttt gactagaaca gttgaggagt ctttacaagc ggtccattac
    20161 acacaacgag aagcaggatt ggctttaggt ttgtcacgct gggaaacagt gataaaagtg
    20221 gtggtttcag aagcgacacc agggattttg actggtgtga tcctaagttc tggaagaatt
    20281 tttggtgaag cagctgcatt gatctatacg gctggacaaa gtgcacctgc gttagatttt
    20341 acgaactgga atccattaag tgtttcaagc ccgatcagca ttttccgtca agctgaaaca
    20401 ttagctgtcc atatttggaa gatcaataca gaaggcacga tgcctgatgg tgtttctgtt
    20461 tcggctggcg cttctgccgt gttgatcatt gctgttttac tgttcaactt cggcgctcgt
    20521 gcgattggca aacgattata ccgaaaaatg acttcagcct aaggagaaga agcaaaatga
    20581 aaaaatataa ctggaatgaa acaaatgtca tcaccatgcc aaaagagaaa gaagtagcac
    20641 tatatacgga tgatctacat gtctggtatg gcgacaatga agcaatcaaa ggagtggatc
    20701 tggaatttga aaaaaataag atcactgcac tgattggtcc ttccggctgc ggaaaatcta
    20761 cgtatcttcg ttcgttgaat cgaatgaatg atgggatcgc taatacgaaa atcactggga
    20821 aaatcatgta tcaaggcgta gatattaatg cgcctcaagt ggatgtttat gagatgcgca
    20881 aacggattgg catggttttt caacgtccta atccgttcag caagtcgatt tatgacaata
    20941 ttacatttgc tttgaaacag cacggcgaaa aaaacaaaaa gaaattagat gaaatcgttg
    21001 aaacaagctt gaaacaagct gccttgtggg accaagtaaa agacagcttg gacaaaagtg
    21061 ccttggcttt atctggcggg caacagcaac gtttatgtat tgctcgtgcg atcgcgatga
    21121 aacctgatat cttgctgcta gacgagccgg caagtgcttt ggaccctatt tcgacaggaa
    21181 cggttgaaga aacacttgta aacttgaaag aagactatac gatcatcatc gtgacgcata
    21241 acatgcaaca agcggctcgt atcagcgact atactgcctt tttctatcta ggaaaagtaa
    21301 tggaatatga caaaactaac aagatattta ccagaccaaa aattcaggca accgaagatt
    21361 atgtgtccgg tcattttggt taagacggag gcctcaacat gacaaaagaa attatttcat
    21421 ctaaagatct tcatctgtat tatggaaaga aagaagcact aaaagggatc gatctaagtt
    21481 tcaatcaagg ggaaatcact gcgatgatcg gtccttctgg ttgcgggaaa tccacttatc
    21541 tgcgttcttt gaaccggatg aatgacctga ttccgaatgt aacgatcacc gggagtgttt
    21601 tatataaaga caaagacatc tatggtccaa aaacagatgt tgttgaacta agaaaagaaa
    21661 tcggcatggt attccagcag ccaaacccat ttcctttttc tatctatgaa aatgtcatct
    21721 atggattgaa attaaaaggg gaaaaaaata aaaaagtcct tgatcaagta gttgaagaaa
    21781 gcctgaaggc agcgtctgtt tgggatgatg tgaaagacaa attgcataaa agtgctttat
    21841 ctttatccgg tgggcagcag cagcgtgtat gtatcgcacg tgttttggca gttaatccgg
    21901 aaatcatctt attggatgaa ccaacaagtg cgcttgatcc tgtttctact gggaaaatcg
    21961 aaagtatgct gttagaatta agagagcggt atacgatgat catcgtgacc cataatatgt
    22021 ctcaagcttc acgtatttca gataagacgg cattcttttt agatggtcac ttgatcgaat
    22081 acaatgatac gaagaagata ttcatgaatc cagaaaaaca agagacagaa gattatattt
    22141 caggtcgatt tggataagga ggaaacggaa tgctacgtac acaatttgaa gaagaattgc
    22201 tgaatctcca taatcagttc tacgaaatgg ggatgatggt gagcagcgcc gtgcacaagt
    22261 cagttcgtgc atacatcaaa cacgataaaa tcttagccca agaagttatc gaaaacgata
    22321 taaatatcaa taacatggaa acacgcttgg agaagaaaag ttttgaaatg atcgcattgc
    22381 aacagccagt tacaactgat ttgcgtatga tcattacggt catgaaagct agctcggatt
    22441 tagaacgtat ggccgatcat gcggtgtctg ttgctaaatc cacgattcgc gtaaaagggc
    22501 agacgagaat accggaaatc gaaaaagaga tctccgatat gtccgattat gtgaaaaaaa
    22561 tggtggacaa cgtcctagtt gcttatgtca aaacagacga aaaagacgca cgtacgatcg
    22621 ccaatatgga tcaaagagtc aatgactatt tcaatcgaat ctataatgca acaatcaaaa
    22681 atatgcagga aaatcctgag acagtcatca gtggaacaga ctacttgaat gttgccagtt
    22741 atctagaacg gatcggagac tatgtgacaa acatctgtga atggatcgtc tacttggcta
    22801 cagggaaaat cactgagtta aatacgaacc acaatgagat tttctaaact aaagcggtga
    22861 ttctgccgtt tgtttaggaa aagaggttgt gaaatcagcg gttgtgaaaa agctgttctg
    22921 catcaagtaa taagaacagt caaacaaaaa tagctcctca tatttttcat ttggctggac
    22981 ttattaccgc agatgcaagc ttttgaacac tgtttattcg ggaaagggtt tgtgacaaaa
    23041 tatgtcacaa acccttttca tcatttcata gaaaggataa agaatgcaaa aaaaaattag
    23101 catcatcact atatttttta tttgtgtgct tacttcatgg gttattcagc atcaagctgc
    23161 tttatatgaa gtacctgtca tcgagatcga aaaagtacga ataaaagagg atcatcaatt
    23221 gattacagga agactgatga acacgaatca aaaaggagaa tcgatttatt tatcggttcc
    23281 ttatcaaaaa aatcaaatag aaacagcaaa tataaaagcg agacaaatgt atctagtcca
    23341 aatgcatgga gatgaatggc agctagtagc aaaaaaaaat gatggctggc ttttcttctt
    23401 cacaagtatc ttcattggcg ccatgttgct tattggcgga aaacatggtc tatttgcttt
    23461 aggaagtctc atcatcaata tcgttttact tttacttttg ttgctcatct ttactaaaat
    23521 agaagggatt tctctattaa atatatctat cctgttcgtc gttataggga tgattattag
    23581 catattggct ttagatggat ggaatagaag cagtataatc aaaatttcag ctgcagttac
    23641 gagtgttttt ttagcatttt ttatttgtct tttgacaatg aatctttgga aagaccaagg
    23701 actgcgctat gaggaactga attatctgac acgaccgtat cgttctgttt ttctttctag
    23761 cgtactgatt ggaacagctg gaggaacact cgataccgtt atcacagtca ttgcgacatt
    23821 agaagaattg actaagcgac aaccagaaat aacagcgaaa cagttatggc tttcgggaag
    23881 gcaagtgggt cgagatgtga ttggctcgat gaccaatatc cttttgttcg tctatatcag
    23941 tgggtcagtt ccatcgcttc ttttatattt aggaaatcaa tggtcgttca aagaaacgat
    24001 agaacagcat ttatcattgg aatttttacg cgtcattgct ggcagcttag gaatagtatt
    24061 atcgatccct atctcggtcc tcttttttct gactgtaagg aggctgaaac gatgaatgta
    24121 cttattatac ttgttgggat tttagcaatc agcgtgctac ttattggcgg tacgcaaggt
    24181 atgtatattc ttttaggctt attcatcaat ttagggatct ttttcttgtt gttattcggt
    24241 tatcatcaga agtggccgat actggcattg tcggtcatag gatttttatt gattgctgtg
    24301 gttatcttat tttttatcaa cggttataat ctaaaaatgc gtgcagcttt tgtgagtata
    24361 ctgatctttt tattttgttt cctactattg cttcctatca ctgatttttt agcaatccaa
    24421 ggttttacaa cgatagaact agaagaatta tctggcttag ataagacttt agcgattgat
    24481 tttagattac ttacgagatc gttgcttctg attagtctta gtggtgctgt tttagatgcg
    24541 agtgtagcaa tttctagtgg gacattcgaa gtatatcagg caaatccgca attatctttt
    24601 taccaattgc gccattctag tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg
    24661 acacttcttt ttgcattcat gggaagttct ttagcgttga tcctctggtt tattgattta
    24721 gacattcctt ttcagcaagt gatcaatgaa aaatcatttg ttctagaata tacgatggct
    24781 attcttacca cattgtccgc actacttgtt cttcctttaa cctgcgtgac tacagcatat
    24841 ctctttacaa aaaagaaaga acaactcaag atggagtaaa agttacaact taagtcctat
    24901 gacattttta ctgaatcagt gcataatatg gatgtaagga aaagcttgca aacaattttg
    24961 cttaaggagg caatttccat gaaagaaaga gaacgtattt tagatttagt gaaaaaagga
    25021 atcctttcga ctgaagaagg cttagattta ttagaaagca tggcaactga aaaagacgaa
    25081 aagcaaatca aaaaagaagc agacaaggta actgctagtc ataaagaaaa agatcaggca
    25141 agtcaattga tcgataaact tgaaaacggc gaagaagaaa tttctgagcc agttgatcca
    25201 aaggaaagag aaagacaaga tcaagaaaac ttagaaaaga tcttagatga actagcaact
    25261 gaagcgaata aaacttcggc gcgtctagat gaagtaaatg ctcaattcgc tgacaataag
    25321 gcagcacgca atgagaaaca ggaagcattg atgcagctga atacaaaaga agaattaggc
    25381 gagttgacag aagaagaatt agcacagcgt cagacgttag aagcagaaat caaagagtta
    25441 gaagctagtg gcgatacact gttagaagaa caattgaaat tagaagcaga attaaaagat
    25501 atccgtaaga atcaatggtc tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca
    25561 gatgattgga aagatcaagc aacagataca ctgaatcaag taggcgaaaa aatgtcagaa
    25621 gctggatcac agttaggaaa attcttgaag aaaacattcc aaactgtttc agaaacagtg
    25681 aatgacaaca tggaatggaa agatgtcagc ttgcgtgttc ctgggattgc gacaacgaaa
    25741 tttgagcatg aattttacta tgaagctcct gcagcaagta tccttgatat caaggcagca
    25801 aacggaaacg tgacgttaaa aacgtgggac agcaacgatg tgaaagttga agctaagatc
    25861 aaactttacg gaaaaatggg tgctgaacca tttgaagcat tctctgaacg gagccagatt
    25921 gaggttaatg aagatcatat ctccttccaa attccaaata aacgtgtgcg tgcagacctt
    25981 gttttctact tacctaaacg agtatacgac cacgcagcta ttaaacttct aaatggcaac
    26041 atcatgattg aaacattgga agctaaagat atttatacaa aatctacgaa tggtaacatc
    26101 atcgtggacc aattaactgc aacgatgctg gaagtagaag gtgtaaacgg caatatcgat
    26161 attcgaaacg ggaacatctt agattctatt atcgaaacag taaatggtac agtgacattt
    26221 ggtgcgacag cagaaaacct ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt
    26281 aaagaagata atttgaaaaa agtagaagca agttcagtga acggaaatgt caaagtcgct
    26341 ttaccagatg gcatcggttt ggaaggacat gctaaaacaa gtcttggaag tatcaacagc
    26401 cgtctgtcca attacgaagt agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa
    26461 ttccgtcgtg tttcagatgg tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt
    26521 atctatttaa aagatacaga taaataaaga ggttgtgaaa gagatgttca gccttgagca
    26581 ataagaagcg attttgtaaa atagcttccc ctattttaca aaattgtgcc ttattgccga
    26641 taggctatct cttgaacacc gtttaccaag agattatgaa cttgccaatt atcgccgagt
    26701 cacaaggaat aatttcaaaa aaagctcgtc acgttttatg aatatggtaa tttgtgaccg
    26761 agaattttgc aaaaagacac cgattaagaa gaaggttgtc acaacgattt tcttcattta
    26821 aaatagtaga acagaggtga gacatgatga aaaaaaagtt gacaaaatcc aataaaaatg
    26881 ttgttttgac agggacttta gcaggaatag gagaatacat cgggattgat ccgactgtta
    26941 tccgtgtgct ttatgtattc ctgagcttag ttgcttttgg aagtccaatc attttataca
    27001 ttttattagc tttgattatc ccatctgcgc gttctgcacg aacaaactat ggacataaca
    27061 atgcatatta tgaaaaaaac aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca
    27121 aagaagcgga aaaaatcgat gatgacgaat ggagtgactt ttaatgtcgt actttcaacg
    27181 tattttagtt aatacgttaa cattcatatc attggctgtt ctttttcctg cgatggtcca
    27241 tgtgagcagt atttggacgg ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt
    27301 tgtcaaaccg attttgacga tactgtccct gccctttaca ttagtcacat tgggattgtt
    27361 tacgtttgtt atcaatgcag cgattcttaa aatgacctcc ttttttgtgg gagcagaaca
    27421 ttttagtttt tcttcattcg gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa
    27481 tgtaattgtc agcgaacata acagtagaaa ataaaagaaa gctgtgccga actatcggcg
    27541 cagttttttt atttagaatc aggaatgcgt tttctattac attggtaatt aaataaaaac
    27601 gtttttttta attggtagta cttttggcta gacaatgata aaatgataga gaacactcta
    27661 atagaaatga ggtagcatga atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat
    27721 tatctttgga aatcgtgaca ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg
    27781 acatctcacg cccaggacta gagttgactg gttattttaa ttattattcc cataatcgtt
    27841 tacagctatt tggcagtaaa gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt
    27901 tgatcatcat gagaagatta tgtagtgaag atatgcccgc atttattatt tcaagaggat
    27961 taccagctcc agaagaaatg atccaggcag caaatgaaaa tgggatagct gtactgcgtt
    28021 ctcccatctc aacttctcgc ttattaggag aattatccag ttatttagat ggtcgccttg
    28081 cccctcgaac aagtgtccac ggtgttttag tggatgttta cggattaggt gtactgattc
    28141 aaggtgacag tgggatagga aaaagtgaaa cagcgttgga gttgatcaaa cgcggtcatc
    28201 gattgatcgc agacgatcga gtagatgttt atcagcaaga tgaattaacg gtgatcggag
    28261 aaccgcctaa aatcctggaa catctgattg aaatccgagg aatcgggatc attgatgtaa
    28321 tgaatctttt tggtgcgagt gcggtacgtg gctctatgca agtccagttg gctgtttatc
    28381 tggaagcatg ggcaaaagat aagaaatacg accgtctggg ctctgaagac acgaccgttg
    28441 aaatcgcgga agtaggcatt ccacaaataa aaatccctgt caaaacagga cgaaacgttg
    28501 caatcatcat tgaagtagcc gcgatgaatt tccgtgcacg tacaatgggc tttgacgcaa
    28561 cgaaaacatt tgaagaaaga ttaagtcgct tgattgaaga aaactcaggc aacgactagc
    28621 aaggagtgac aatatgttag cacaaatcaa tcgagttgct tttgagcttg tagggattcc
    28681 tatctactgg tatgcattga tcatcgtatc aggtatcatt atcgccatgt ggcttagcag
    28741 cagagaggct gttcgggtag gattaaaagc agatgatgtg acagatttta tgttgtgggg
    28801 attgcctatc tccattattg gcgctcgctt atattatgtt ttatttgatc ttccccagta
    28861 tatagctgat ccaatccaaa tattcaatat ccgttcagga ggcttagcta tttatggcgg
    28921 actgattgca ggcgcgatca cgctgtattt ttttactaaa tatcatttta tttctttgtg
    28981 gactttttta gatatcgctg caccaagtgt attattagca caagcaatcg gtcgttgggg
    29041 aaatttcatg aaccacgaag catatggacc agacacgacg cgaagcttct tagaaagtct
    29101 gcatttaccg caattcatca ttgataatat gtatatcgaa gggatctatc gtcaaccaac
    29161 atttctttat gaatcacttt ggagtttgat tggatttatt ttgcttttgt ttttacgaaa
    29221 gaaaccacac cttttgaaaa aaggtgaagt ccttttatgt tatctaatgt ggtatagttt
    29281 cgggcgtttc tttattgaag gattacgtac agacagtctt tactttttag gtgtgatccg
    29341 tatttctcaa ttattatctg ctgtgttatt tgtcggtaca ttgattctat ttatttggcg
    29401 cagaaaaaaa ggaaatttac cggattatga ccgctcatta ggaaaaaatc aacaaattat
    29461 ctgattttta gagcgttaaa aatgaaaaaa atttcaggag tggtaaaatg aattatagag
    29521 tagcggtttt aggaccaggc tcttggggta ctgctttggc acaagtcctc gccgaaaacg
    29581 gacatgacgt tcggatttgg ggacatcgtg cttcacaagt agaagaaatc aacacccaac
    29641 acacgaatca acgttattta ccagatcatt gtctacccga ttccatccaa gcattcacca
    29701 atatggaaga tgcagtaaaa gacgcagatg cggtgctatt cgtcattcca acgaaagcaa
    29761 tacgttctgt tgcaaaagaa ctagtgccta aattgaatac aaagccagtg atcatccatg
    29821 caagtaaagg attagaacag gggacacata aaaggatctc tgatatcttg ttagaggaaa
    29881 tcccagtaga aaaaagaaaa gctgttgttg ttctttctgg accaagccat gcagaagaag
    29941 tagcgaaaca tgacatcact acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat
    30001 atgtacaaga gctgttcatg aataattatt ttcggatcta taccaatcct gatgtgatcg
    30061 gtgtagagat gggggcagct ttgaaaaata ttattgctat tggtgctggc gctattcacg
    30121 gcttagggtt tggcgataat gcaaaggcag caatcatgac gcgcggatta gctgagatca
    30181 gccgtttagg tgtagcaatg ggtgccaacc cgctgacctt tatcggatta agcggagtag
    30241 gcgatctagt cgttacttgc acaagtgttc attctaggaa ctggcgcgca ggcaacttgt
    30301 taggacaagg gcagccccta gaagaggtgc tagccaatat gggaatggtc gttgaaggtg
    30361 tgaatactac ccaagcagcg atggaactag cccaaataat ggaaatcgaa atgcccatca
    30421 ctcaaacaat ctatgaagtg ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa
    30481 tcatgttacg tgatgggaaa aaagaaaatg aattctcatt aacaattttt tgaaggagcg
    30541 aatgtatatg aaagtcaaaa aagcagttat tccagcagca ggattaggaa caagattttt
    30601 accagcaaca aaagctatgg caaaggaaat gttgccaatt gttgataaac caacaatcca
    30661 gttcatcgtt gaagaagcgt tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa
    30721 agctaaacga ccaatcgaag atcactttga ttcaaatatt gaattagaaa caaacctaag
    30781 tgaaaaagga aagaccgaat tattgaaatt agtagaagaa acgacagatg tcaacttgca
    30841 tttcatccgt caatctcatc caaagggttt aggacatgcc gttcttcaag caagagcctt
    30901 tattggaaat gaaccatttg tcgttatgct aggcgatgat ataatggaag acgaagtacc
    30961 attgacacag caattaatcg atgattacga acgtactcat gcttctacga tcgccgttat
    31021 gcaagttcct catgatgaaa cttcaaaata cggaattatc aatccaggag aagtattgga
    31081 agatgggtta tacaatgtca aaaactttgt tgaaaaacca gatccaagca aagcaccaag
    31141 tgatttagct attattggac gttacttatt gactccagaa atttttgatg tattgtccga
    31201 gcaacaacca ggagccggaa atgaaatcca actaactgac gcaatcgata cattgaataa
    31261 aacacaacgc gtatttgccc gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt
    31321 tggttttatg aaaacaagta ttgaatatgg tctaacacat ccagaagtag gtgcaccttt
    31381 acgtgagtac atcctgtctt taggtaaaac attagaagca gaaggaagca agatcgatca
    31441 taatccaaaa ggaataacag aaatcaaaga agaaaagaaa aaataaccct tttttctaac
    31501 gagcttcagc tgctaattga ataaaaagta gttgaagctc gtttttattt gtttaaaaaa
    31561 cacgaaaatg ctatccttat agaaaagaga gacaagaagg gagtttgatc atgtcaggag
    31621 gagaaattgc aggattgatc gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc
    31681 gtgtgttgtt gcaagtttct aaaactttag aaaaagtaga tcagactgta gcagaagcaa
    31741 atacgacgat cgaaatcgtc acaaaagacg tagatctttt gtctagacaa gtagaaggct
    31801 tactggtcaa aagtaatgaa ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc
    31861 cattgttcac agcagttgct gatttaagtg aaagtgtatc agaacttaat cattctagta
    31921 aaaacatcgc gacgaaagtc ggcggaatcg gtaaaacgac agctaaagcg acggttgccg
    31981 gaaaagtcgg tgggacagcg atgaaatttt ttaaaaacaa aaaacatact gaaacgacag
    32041 gaggaaaata aaaatggcta aaaaaggtgg atttttatta ggtgcagtag tcggaggcac
    32101 agccgcagca atcgccgcgt tattgttagc accaaaatct ggtaaagaac ttcgggatga
    32161 attagctgcc caagcggatg atttgatgga tatggcttca gattacacag atatggcgaa
    32221 agaaaaatcg actgagttaa caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca
    32281 agcaggtgat ctagcaggta gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg
    32341 aagtgaatct gacgatatct tgttaaatct taaagaacaa tcagctgact tatctaatcg
    32401 tttcaaaaaa acggcagaag atatgaaaaa acctgcagat gaattaggaa gtattgctaa
    32461 agatacttca gaagatatca tgattgatgt aaaagacgct gctggtaaag caggagatac
    32521 tgtcaatgaa ggaatgtcag aagcaaaacc agttgcagat gactcaaaag aaatgatgaa
    32581 aatggcaaaa gaagatgcga aagaagctaa aaaagaaata aaagatacaa tgacagattc
    32641 atctacaaat cactaaaatc acaaatttat agaataaaaa ggtgggagcg gactagctct
    32701 cacctttttt actcttattt ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc
    32761 acacagccat cgcttgtcac atatacacag tcttcgatac gtacccctac tttttcagga
    32821 atatagattc ctggttcaag agagaagcac atcccttctt cgattactaa gtcatttcct
    32881 tctacaaggg aagggaattc gtggacggtt gttccgatcc cgtgacctaa acggtgattg
    32941 aaatattcgc cgtagccata gctgttgatc acgtttcgtg cgatttgatc aagttcacct
    33001 gctgtcacac ctggacggac ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg
    33061 tagatttttt cttggaagtc ggttggtttt tgataagcaa ttgtacgagt agcatcactg
    33121 caatagccat tccaaacaac gccaagatcg aataacacaa gttcattcgg tgaaatcttc
    33181 gtatttcctg gtgtaccatg agggcttgca gcatttgctc ctgcaaggac taatgtgtcg
    33241 aaagacattt gtgaaacacc ttgttttttc aattggtatt caatttctgc aatgatcgct
    33301 tgttcagtca cgccttcttt cactgcttta aagccaatct caaatgcaac atcggcccat
    33361 ttaccagctt cgatcagttt gtctttttcc tctagagttt tgatcagttg tagacgttgg
    33421 atgatggaag ttagatcagt gtaagaatcc gttcccgtca agatatcaga gatagcatcg
    33481 aagcgctcga cagtcaaggc gttcttttca atccctacat tgccaggctg accatagcga
    33541 gcacgaagtt cttttccgat gatctcccaa gggttttcgc tgtctaaata gccacggaca
    33601 tcatacgccc aactactgtt ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa
    33661 ggatcttgtt caagagggat aaaaagagct agtacacgct catgaggatc actttcgtat
    33721 ccggaaaagt aagcgatgtg gccagggtcg ctgatataag ccaattcgac ttggtgttgt
    33781 gccatccatt gacgtagttc attgattttt acgttattca tttggcaacc tactttctaa
    33841 attaattttg tattcaataa ttgtaccact gatttattaa aagaccaacg ttctaaaaac
    33901 tgaaaaaagt aaaaaatttc atgaaaacag agataaaacg gttgcaattc ttgagattat
    33961 ttgatattct attttagaaa tgtaagacgt attttcagaa aacatataaa atatgaatag
    34021 gagaacacaa atggaaaagc aaacaatcac tatctatgat gtagcgagag aggcaaatgt
    34081 ttcgatggct actgtttcac gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag
    34141 aaaaaaagtc ttggaagtta tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg
    34201 cttggcaagt aagaaaacaa caacagttgg tgtaatcatc cctgatgtca gcaatatgtt
    34261 ctttgcttct ttagcacgtg gtatcgatga tgtggcaaca atgtacaaat acaatattat
    34321 cttagcaaat tcagatggca acgaccaaaa agaagtcaat gtattgaata acttactggc
    34381 taagcaagtg gacggcgtga tcttcatggg tcaccatatc acagacgaaa tccgtggaga
    34441 attttcacgt tcaaaaactc ctgttgtatt agctggctct attgatccag atgaacaagt
    34501 aggtagcgta aacattgatt atgtatccgc aacaaaagat gcagtaaaca aattggcaaa
    34561 aagcggcaat aaaaaaatcg cttttgtcag tggtgcgttg atcgatccca tcaatggaca
    34621 aaatcgtctg aaaggctata aagaagcatt aagcgaaaat aacctaccat atagcgaagg
    34681 attgatcttt gaagcacaat acaacttcaa agatggtcta tctctagtcg atcggatcca
    34741 taatagcggt gcaacagctg tctatgtttc agatgatgaa ttagccatcg gtattttgga
    34801 tggtttatta gaccgtggcg tgaaagtacc ggaagaattc gaaatcatca caagtaacaa
    34861 ttcactactg acagaagtag ctcgtcctcg tttaacaagt atcacacaac cattatacga
    34921 tttaggggct gtatctatgc gcctattgac gaaaatgatg aataaagaag aagtcgaaga
    34981 aaaaacgatt atcttgcctt atggaatcga agaaaaagga tctacaaaat aataaaagga
    35041 caaccattca gaggttgtgt gcctgccgtt taactctgcg ttacaaggag caatttcaaa
    35101 aaacagcatc tcatgtttga tgaatattgt gactcgtgat tcggagttgg caggagaaca
    35161 ccgtttattt gggaaaaggg gttatgacaa aattttgtta taatcctttt tttgtgtctt
    35221 aaaaaagaga gaaagtggtt gaaattacat ttatcgtata taaaacaatc cgtgtataga
    35281 tgctgcattt tgttcaccac catcaaaaaa ataagggact gtgacaatac tattgtcaca
    35341 atcccttatt actgtgtatt ctattaaatc aatcatcttt ctcatctgat tctttagttg
    35401 cttgattcgc tcttgcataa gctgaagcgg tgttccagta atctgtatag ttttcatcgg
    35461 taccgccaac gccaaattta aaggcgcttt tcttcggtcc atttttcgcc catagcgatt
    35521 caactgtttt tcctggtgtt tggatggatt ttttattgac agtaactttt ccaggtgttt
    35581 gaccagtaaa tccagatact tttttcttca tcacatcaga tgataattcg aatttctcat
    35641 cagtgccaaa aatgtcaggt tttgcttggt aagcttgatt gatcagattg gcaagataat
    35701 tagccgaacg tatccttgct ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc
    35761 tactgatggt aatagacgga gtagagacaa ttagccatga atctcggtat tcattggtag
    35821 aaccagtttt tccgacccaa tctgcttttc ctagatttcc gtttaagctt gagatggcat
    35881 ctttgaatgg tgtagtgatt tttgcattga tcacactacg catcatgtca ttcatgatcg
    35941 atgccgttgc ttccgaatag atccggacag agttgctttc gtgtttataa attacatttc
    36001 ctgcgttatc tgtgatggaa tcgatgatat agccttcttc atagacgccg ccgtttgcta
    36061 aagcctgaaa tccgttggtc tgttgaagcg tcgtgacatc tactgtacct aaaggagcag
    36121 attcaacgcc ccaattgtca tttgccgggt agttcatctt tgccaagtag tgctcgtaag
    36181 catattgttt cgatccaccg gaattcaaca catcttgata taagtgataa gccgggatat
    36241 tgttggacca ttctagtgac tcacggacgg tttggaaggt gttcgatcct tcgttagtag
    36301 cattgacgat tttctcgcca gcattttctc cttcttgcca agttgttgca taatctgata
    36361 cacgggattc tgatccgata agccccatgt cgattgcagg tccataaaca agaactggtt
    36421 tgattgatga gccagcttga cgttctgtat cgaaggcatg gttattttga ttctgactgt
    36481 aattccgtcc gccaacgaat ccgtagatgc ggccagttct gttatccatc aatacatttc
    36541 cggtttctac ttgactgcca gcaccatcat ctaacatgta tccataattt tcaacgccat
    36601 tttgcaatgc agcatagaca tctttatcga ttgtcgaatg gattgtatat cctttatttt
    36661 ggatcgtttg ctgtgccttt tgataataag cgtcactgac cgatgaatca gacatatcag
    36721 cgttgtcttt ttcagccaat tgttgtgcaa tgattcttgt tgcttcattc atcacagtat
    36781 aatacaagaa ttctctgtca gtctgttctg cgatttgttg tggtagaaag tcttttgtaa
    36841 ggtcataggc ttttgcttct tcgtattgtt ctttcgtaat ttggccttca cgatacatac
    36901 tgaataaaac aaaatctttt ctggcaaggc cggcagaaag atcttctttt agcgcgcctg
    36961 tgttcgtgta tggactatac gtgatcgggc tttggggtaa tccagcgata taagcagctt
    37021 gcggcagcgt cacatctttt gcatctacac caaagattcc ttttgcagct tcctggactc
    37081 cagcaatgtt ttgtccctta ttattacgac caaatggtga gacattcaaa tacgtagcaa
    37141 tgatctcatc ttttgaaaag tatttttcta cttgtgcaga aagaatgatc tcgtttgctt
    37201 tacgtttgaa tgttgtttca tctgtaagga tctgctgttt gactaattgc tgtgtcaaag
    37261 ttgatccacc agaagagcca ataccagtag cttcagatac taatgctcgt aaaacagctt
    37321 taggaacgta accttggtgt ttatcgaagt attcatcctc tgtgctgata attgcagttt
    37381 tcaataaagg tgaaatctta tcagatgaaa tagtcgtgcg catcagatct gtctggattt
    37441 ttgatatctc tgtattatct gcataaacaa tctttgacgt ttctgtaatg tttcctagtt
    37501 ctgtctgcag tgctttttta gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc
    37561 cagcgcctat tccaatacct agagctgcga acatcaaaag aagaacagta ataaaaacag
    37621 tcaatgattg aaaaactcta aaaataatct taggaacaaa ccatttttct ttttttcttc
    37681 tattctgttt ttttcgagta tttttgcgtg gcaaaagggt cacctcaact aatggtttta
    37741 tttcgctaat tatagcaaat aatagaacaa aaaaacagac aaggctgttt ttggcaggaa
    37801 tttctttttt aaaagagtta aaa
//
LOCUS       DAZUMV010000015        37276 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000015 DAZUMV010000000
VERSION     DAZUMV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.015"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(166..1422)
                     /gene="tyrS"
                     /locus_tag="ACG8FK_000680"
     CDS             complement(166..1422)
                     /gene="tyrS"
                     /locus_tag="ACG8FK_000680"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS5096312.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(1534..1739)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2002..3303
                     /locus_tag="ACG8FK_000681"
     CDS             2002..3303
                     /locus_tag="ACG8FK_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5096313.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(3372..3971)
                     /locus_tag="ACG8FK_000682"
     CDS             complement(3372..3971)
                     /locus_tag="ACG8FK_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5096314.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            4226..5107
                     /locus_tag="ACG8FK_000683"
     CDS             4226..5107
                     /locus_tag="ACG8FK_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5096315.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            5120..6730
                     /locus_tag="ACG8FK_000684"
     CDS             5120..6730
                     /locus_tag="ACG8FK_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096316.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAKGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(6807..7466)
                     /locus_tag="ACG8FK_000685"
     CDS             complement(6807..7466)
                     /locus_tag="ACG8FK_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5096317.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(7749..8414)
                     /locus_tag="ACG8FK_000686"
     CDS             complement(7749..8414)
                     /locus_tag="ACG8FK_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS5096318.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(8561..9166)
                     /gene="dhaL"
                     /locus_tag="ACG8FK_000687"
     CDS             complement(8561..9166)
                     /gene="dhaL"
                     /locus_tag="ACG8FK_000687"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS5096319.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(9194..10204)
                     /gene="dhaK"
                     /locus_tag="ACG8FK_000688"
     CDS             complement(9194..10204)
                     /gene="dhaK"
                     /locus_tag="ACG8FK_000688"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS5096320.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(10179..10574)
                     /gene="dhaM"
                     /locus_tag="ACG8FK_000689"
     CDS             complement(10179..10574)
                     /gene="dhaM"
                     /locus_tag="ACG8FK_000689"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS5096321.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            10728..11048
                     /locus_tag="ACG8FK_000690"
     CDS             10728..11048
                     /locus_tag="ACG8FK_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS5096322.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(11160..12017)
                     /locus_tag="ACG8FK_000691"
     CDS             complement(11160..12017)
                     /locus_tag="ACG8FK_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5096323.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(12125..13429)
                     /locus_tag="ACG8FK_000692"
     CDS             complement(12125..13429)
                     /locus_tag="ACG8FK_000692"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5096324.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(13426..13935)
                     /locus_tag="ACG8FK_000693"
     CDS             complement(13426..13935)
                     /locus_tag="ACG8FK_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096325.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(14110..16245)
                     /locus_tag="ACG8FK_000694"
     CDS             complement(14110..16245)
                     /locus_tag="ACG8FK_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5096326.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(16328..17983)
                     /locus_tag="ACG8FK_000695"
     CDS             complement(16328..17983)
                     /locus_tag="ACG8FK_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5096327.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(17985..18299)
                     /locus_tag="ACG8FK_000696"
     CDS             complement(17985..18299)
                     /locus_tag="ACG8FK_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5096328.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            18469..18729
                     /locus_tag="ACG8FK_000697"
     CDS             18469..18729
                     /locus_tag="ACG8FK_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS5096329.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            18743..19057
                     /locus_tag="ACG8FK_000698"
     CDS             18743..19057
                     /locus_tag="ACG8FK_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS5096330.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(19787..20341)
                     /locus_tag="ACG8FK_000699"
     CDS             complement(19787..20341)
                     /locus_tag="ACG8FK_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS5096331.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(20467..22396)
                     /locus_tag="ACG8FK_000700"
                     /pseudo
     CDS             complement(20467..22396)
                     /locus_tag="ACG8FK_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(22702..23418)
                     /locus_tag="ACG8FK_000701"
     CDS             complement(22702..23418)
                     /locus_tag="ACG8FK_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5096332.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(23419..25242)
                     /gene="glpO"
                     /locus_tag="ACG8FK_000702"
     CDS             complement(23419..25242)
                     /gene="glpO"
                     /locus_tag="ACG8FK_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS5096333.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(25256..26752)
                     /gene="glpK"
                     /locus_tag="ACG8FK_000703"
     CDS             complement(25256..26752)
                     /gene="glpK"
                     /locus_tag="ACG8FK_000703"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS5096334.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(26888..28348)
                     /locus_tag="ACG8FK_000704"
     CDS             complement(26888..28348)
                     /locus_tag="ACG8FK_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5096335.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(28338..29633)
                     /locus_tag="ACG8FK_000705"
     CDS             complement(28338..29633)
                     /locus_tag="ACG8FK_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5096336.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(29965..30642)
                     /locus_tag="ACG8FK_000706"
     CDS             complement(29965..30642)
                     /locus_tag="ACG8FK_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS5096337.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(30672..31763)
                     /locus_tag="ACG8FK_000707"
     CDS             complement(30672..31763)
                     /locus_tag="ACG8FK_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS5096338.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(31864..32298)
                     /locus_tag="ACG8FK_000708"
     CDS             complement(31864..32298)
                     /locus_tag="ACG8FK_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5096339.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(32882..34339)
                     /locus_tag="ACG8FK_000709"
     CDS             complement(32882..34339)
                     /locus_tag="ACG8FK_000709"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5096340.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(34336..36231)
                     /locus_tag="ACG8FK_000710"
     CDS             complement(34336..36231)
                     /locus_tag="ACG8FK_000710"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5096341.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(36289..>37276)
                     /locus_tag="ACG8FK_000711"
     CDS             complement(36289..>37276)
                     /locus_tag="ACG8FK_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5096342.1"
                     /translation="PYSFSNQEKQAIENRLLELNYLMINDEKNKEIEKNLRAFEQTII
                     RFLDLPVSLLFHETEFLLTHIKQMKTRMDAGHNTMNHFVQKIISRYPLEIYLIRRFFP
                     IHFQLRPNLAESAYFVLYLAEALNPFRKQGEILIVSNQPISILNTLKKQIQQSMNMWI
                     KECRIEPVYLFKNQSNKITEYDAILTTEQELIFEWDDLIYLPSVLDEFESWEIYQILR
                     EKLTIVEEKKKTKLLDHYYKEENQIKIHKKIDSIQQLISPSSDLVMLPLNAETLLCCL
                     IKSQVETKITEYQLEIPLIHQQRKIKRVFFVSFDYKRNDILVFFQAVSDLLG"
BASE COUNT        10477 a         7894 c         6465 g        12440 t
ORIGIN      
        1 ttgttcacac ttttgaaata attaaaaaaa acgagggaat ttacaaatgt aaactccctc
       61 gcaaagattg tattaacgac aaccagcgtt ctattttagg atgggtttca aaattggaat
      121 gttgtaatag tatttccctt tttcaaggta aaaacttact aaattctaat caattacttt
      181 ggccaaaaag taatttttct taccttttcg aataaccaca aacttaccat cgaattcatc
      241 ggatggattt atgacaaaat ttaaatcggt aacgcggtca ccgttaatac ttatagctcc
      301 gtttgaaaca tcttcacgtg cttgacgttt agatggttca atttttgtgg aaacaagcag
      361 ttccacgata ttttcaggtg tgcttgaaat ttcaacgttc ggcattttac cgaatccttg
      421 cgcgatttct tccgcattta gttctttaat atttccagag aacaatgctt gcgttatttt
      481 ttgcgcttct ttcaagtctt cttcactatg cacaaatctt gtaacttctt ctgccaaacg
      541 gcgttgtgct tcacgtttct caggttctgt ctctactttt ttcgctaagt cttcgatttc
      601 ttcttgagac aagaaagtaa agaatttcaa gtatttcact acatcgcgat catcttgatt
      661 caaccagaat tggtagaact caaatggtga agttttcttc ggatcaagcc aaacggcacc
      721 gcctgcagtt tttccgaatt tcgttccgtc agccttcagc atcaatggga tcgttaaacc
      781 gaacgcttct gcttctggtc cttctaattt tcggattagg tctaatccag ctgtgatatt
      841 tccccattgg tctgctccac cgatttgcaa ttgcacatcg tatgttttat gcagatgcaa
      901 aaagtcgata gattgcagga tttggtaagt aaactctgta aatgagattc ctacttccaa
      961 acggctggca acgatatctt ttgctaacat cgtgttgatg ttgaagtttt taccgtagtc
     1021 tctcaaaaat tcaagtaaag agatttttga caaccagtcg tagttgttca caaatgtgat
     1081 atttgcatct ttaccaaaca attttctcat ttgattggaa agtgcgtcca cgttatgttg
     1141 cacttgttcc atcgtttgca acacacgttc tgttttgcgg ccgcttgggt cgccgatcgt
     1201 tcctgttccg ccgccaatca gaatatatgg atgatggcct gccaattgga atcttttcat
     1261 catcataaat ggaatcaaat gtccgatatg catcgaatca ccagttggat caacgccgca
     1321 ataaagtgaa atgctctttt cttcaacgag ctcgcgaagt ctttcttcgt ttgtttgctg
     1381 attaatggcg tcgcgccaag ttaattcatc aataatattc attttactga cctccgtgta
     1441 gtttttttat gaaagcagga acctgctagc atgtgtaatt aattatttct tcaggatagg
     1501 ataggaaata aaaaaagtcc cttggcatat aagccaaggg acgaaatctc gcggtaccac
     1561 ccaatttgta tgtctagata tccaacatac ctctcgaaca tttaacgctg tcgcgtggca
     1621 atcactgcca aagactcctg aaatgtaatt cgctttaaag catgtactgg tttgcagcga
     1681 ccaccagctt tctgaaacat tggctttaac aactacttcg tttcgatctt cgtcttgtta
     1741 ttattaatat gtgtctatca tagagcgcaa ctttttcttt gtcaacagaa aaaggatatt
     1801 ttttttaaat tcctcatttt ttcttttttg aatccgctct aattccgaac attttatttt
     1861 caattattgt taaaagaaat aactttttct gtattttttc tgcaatttat cgagaagtca
     1921 ctttactttg cctcttattc tttcttcgtt acaataagga atgggacaag taaaaaaatg
     1981 aaaaagcaag ggacgaggta tatggaaaaa aagcaaaaaa ttattttagg agccacttct
     2041 gctttaacta tcgttatttt aattctaagt ttgttttttg tcatccgatc atctacaaaa
     2101 aaagaaccaa tcattcctga aaaaaaaata acagaaaaag caaaagaacc gacagatgat
     2161 aagcaacaac tagttactca agcaaaaaat ctggctcaaa gctacgatta tgaccaagcg
     2221 attgcgttat tggaaaaaga tagtaagaac ggatctactg aaacacaaca gcttcttcaa
     2281 acactaaaaa aggagaaaga acaactgatc gactggtctg atcctactca gatctctcat
     2341 gtctttttcc acagcttgat cgtggacccg caaaaagcct tccactctca acaggcacaa
     2401 ggatacaaag attatatggt gactgttgaa gaattcaacc gttcgatcga acaactttat
     2461 caaaatgatt ttgttttagt taatttgaat gatttgatcc aaaaagacga acaaggaaat
     2521 ctttccttta acgggcttgc gctacctgaa ggaaaaaaac cattggttct ttcacaagat
     2581 gatgtcagct attatgaata tatggataat tccggttttc ctagccgatt ggtcgtatca
     2641 aataacaatg aaatcaaaaa tgtctataaa gagaaagata aagaagaaat cggtgacttt
     2701 gacatggttc ctctgatcga tgcctttgtc aagaaacatc ctgatttttc ttatcgaggc
     2761 gcaaaaggta ccttggccct gacaggttac aacggagttt taggttatcg aacatcaaaa
     2821 tctcaatatg gagacaatga aaaaacaaaa aaagagatta aagaagctaa aaaagtggcg
     2881 gaacagttga agaaagacgg ctggagcttt gcttcccata catggggtca cttgaatatg
     2941 actcaagctt cactagaaga tgtaaaaaaa gacaatgaat tgtggcagca agaagttgca
     3001 cctatcctag gaaaaacaaa tattctgatt tatccatttg gtgcagacat cagcgactgg
     3061 cagccttatt cagcagaaaa tcaaaaattt gcttacttga agagtcaagg tttcgatatt
     3121 ttctgtaatg tcgatgcttc tacaccagct tggggacaat taggtacgga tttctatcga
     3181 aatgctcgga tcaatatcga tggcattcgt tttcaatctg atttagacgg aaaaaatcct
     3241 attcttgatc cattcatcaa tgtccatgat gtctatgaca aagaagcacg cgaatctgct
     3301 tgattttacg tcatttatga accaaaaaag ccgaactgac attttatcgt cagtttcggc
     3361 ttttttatgc tttatttctt cttcggtgcg attcccttga tcaggacttc tgtccattct
     3421 gctgtatatg gaagcagttt tgcgtatatc tccggcttag aaattccttc taccattgct
     3481 tgaaaataga ggatcaagaa ctcatccgaa tatttcaaat ccacttttcc ttctttccgc
     3541 cctcgatgga aaagatcaag catgatactg aaactttctt gcgtatacgc atccatcgcg
     3601 tcagaaaatt cttttttttc tttttttgta tagtatgtca tcaaatcgat ataaaattgt
     3661 ttgttcactc gattgagata acggattttg ttttcactca tggcaatcag tgtttcttca
     3721 aatggaagat tttccgcaat caacgctttc gcttcttgcc ctaaatcatt cataaatgca
     3781 aaaaatactt cataaatcag cccttccttg ctgccaaaat atttaaaaat cgtcgcttta
     3841 cttacctgag cgttctcagc taccttatcc attgtcactc cagcggttcc tatgtctgtg
     3901 ttcatcaagt caaaagctgc ttgtaaaatc tctttctttt tctcttgtgc tctcttttca
     3961 aaaccgttca tttgtagagc ctcccgtaat tttaaacttt tataatatat atagttcaaa
     4021 tttattctga ggtttattat aactatctat tgagaaagta tcaagctagc aattccttgt
     4081 ttatgaattc attgacagaa atttataaaa agaatattat gtactaaaat aataaaaaaa
     4141 agttttataa aaaggtataa aaccttttta ttaaactatt taatcttgtt tagtatatat
     4201 ttttagaaaa aggagtgggg tttttatggc agaaatcgtc aaagtcacac atttgaaaaa
     4261 gagctttggt cgtttcgaag ctttaaaaga tgtctctttc accgtacatt caggtgaagt
     4321 cgttggattt atcggaccaa atggttctgg aaaatccacg acgattcgga cacttttagg
     4381 gattataaaa aaagacggcg gaacggcaac gatttttggg caagatgtat gggaagatag
     4441 tttatctatc catgaacgtc tttcttatgt tccaggcgat atctctttgt ggggaaattt
     4501 gactggagga gcgatcatcg atctcttcat gaagcttcat ggaaaaggga gtgctcaaaa
     4561 aaaagatgaa ctgattcaac gtttctctct tgatcctaag aaaaaagcaa aaaactattc
     4621 aaaaggaaat cgccaaaaag tgggcttgat tgctgctttg gctgttgatt cggatctcta
     4681 tttatttgat gagcctactt ctggtttaga tcctttgatg gaaacgattt tccaagaaga
     4741 agttgaaaaa ttgaaagaac agggcaaatc catcctactt tcttctcata ttctcagtga
     4801 agtcgagcga ctagcggata aagtcgtgat catacgcgaa ggaacactcg atgaagaagg
     4861 aacactcgat gaattgcgtc atttgactcg ttcgaccatc actatagaaa cagaagcaga
     4921 gaatagtcac ttagcacaat tgaacggtgt ccatgacttt gtgcaaaaag gtcacctcgc
     4981 tcaatttgct gctgataacg aagctttcga tgtgatttta gctgaagcgt ccaaattagg
     5041 tgtgaaaaaa tttgaatcta ttccaccgac tcttgaggat ttattcatga gacattatga
     5101 aggatgagga ggaagcgaaa tgactgataa attttcacgc tggtccaccc ttttttctca
     5161 aaaccttagc cgtgactgga aaaagatcct gatatggcta gtcggtgtca ctgctttttc
     5221 tggtggtttt gtccctgcgt ttgaagaatt gacttccgga caaggattag ctggattata
     5281 cgaaaccttg caaaacccgg caatgattgc aatggtcgga ccaactccag cttcgaatgc
     5341 tgctgagtat actttaggag cgatgtatgc aaatgaaatg ttgctttttt gcagtttgtt
     5401 tgctgctgtg atctctgttt tacacgtggt gagccaaacg agaaaaaaag aagaacttgg
     5461 tctgattgaa cttattcgtt cctttcgagt cggacgtcaa gcaaactctt tagctgtcat
     5521 cgctgaaagt attctgatca atttgctttt agcaatatcg atcagctgta cgatgatttt
     5581 tttccaagcg gattcgatca ctgcaaaagg tgcttggctt tttgggctat ctgtaggtag
     5641 tatggggatt ttggggagtg ccgttgcact gtttgctgca caactgatgc caagttcagc
     5701 cagttcgaat ggtctgtctt tagcgttttt aggtggactt taccttctac gagcaataac
     5761 agatgtagct gattcctccc tttcgatgtg gaatccttta ggctggtctt atttgaccta
     5821 tccatttaca actaacaact ggattccttt gctttatttg tttttgttca gtctaatctt
     5881 gataatcagt tcatttgttt tagaggaaca tcgagatttg aacaccggct atatccccga
     5941 acgtgaagga aaaccgtatg ctaaacgttc tcttttatcc ttgcgtggtt tattattcta
     6001 tctaaataaa gggatgacaa tcagttggct tatcactttt ggtttgatgg gggcggcata
     6061 cggctctatc tacgggaata tgcaacagtt tttagaaagt aacgacttaa tgaaacagat
     6121 gtttatccaa tctggcgtct caattgaaaa aaactttaca agcacgatca tggtagtctt
     6181 gatttgtcta gctgctattt taccaattgc tagcatcaat cgtttattta cagaagaaac
     6241 ccacggacgt atgaatcagc tgttcgcaac aaaaatttct aggaaccaac tttattggac
     6301 caatatgtta ctagctattc tatttgctat attggcaatc ctgattagtt ctggtgcact
     6361 agggctttct gctttggctt cgatgaagga tgcgaaagac atggatctat tgatgtttct
     6421 taaagccggg ttcaatttcc tgcctgccgt tttagtgttt atcggaatta gtggctttct
     6481 attcggatgg cttcctcgaa tcggtaaact tgcttatatc tacttagctt actcctttgc
     6541 actcaattac tttggcggaa ttttggattt accagaatgg ttttccaaaa cagctgtccc
     6601 tagctgggtt ccacggatgc caaacgattc ttttgatctt tccgtgttcc ttacgttatc
     6661 atgtatcagc cttgcactgt tcgtccttgg ttctattggt tataggcgaa gagacttact
     6721 agaaggataa taaaaaagca tgtacaattt catgctagcc tccaaatctg ggtcaaaaat
     6781 ccaacatttg gaggtcattt ttttatttac aagtcttcta actgctcttt atcaatgatc
     6841 cagacttctc gattctttct ttttacataa ccattctcct ctaataattt aaattttcta
     6901 gataaagttt cgggggtcgt accgataaat gcagcaagtt ctttcatttt cattggcagg
     6961 aaaaagtgat ccttccctgt cgctttagct aagtcaagca gatagacagc gactcgttct
     7021 tctacttttt ccatcatcaa aaaagttgtc tgctgctgta cttttgttaa tttctcaacg
     7081 ttgattgcta aaagtttcag actaagggcg ggataatgca gtaagagttc cgagaaatct
     7141 ttttgtctca atatgcaaat ttccgttttt tctagtgctt cgccaaataa agactcattt
     7201 ggaacgccta gcaactggct ctctccctca tatccgcccg gctctattac tcgtaaaagc
     7261 tgttcctttc cgctagcaga taattgatac actttcatac tgccggaggc cacgatcact
     7321 aattgaggtt ctccattcgg cgcaagaatc tgttctcctt tttccacata gtgatgtttg
     7381 acaagtgcgt gtactttttc ttgatcttgc tgtactaagt gattgaacaa aggaacaaat
     7441 gaaacacata aatgatcatg actcataatc tctcctcctt ctaaacagct atttctaata
     7501 aagaaacata acttccctta ttccaactga taattttctt ttatcatagc ataaacgtca
     7561 agcgaaccag ataccagcaa atagagtgga tttctttttg tcccttgctt tattttcctt
     7621 cttttgagac cttaagtaat tgattcctat caatttttta aatatttact ttttgaataa
     7681 tcaaaaacaa accttcactc gcaagccgag agtaaggttt gtttttcgca ttgcttattt
     7741 cgtgttgtct attgaaattt ttccgcacct agcttttgat atagtggtgc tagttcttgt
     7801 aaggtttcag cggttatttg ctcaacgtgt tcgatttctt gcggatagaa gaatagttta
     7861 cccatttgaa aggttcggtc tctcatcgct tttttcaact cttcttgttt ctttgtatcg
     7921 gacaaatagt cagacaaagc taagtggtcg tcaagttcat gttctggttg cggccatatg
     7981 taataatcct tgatatcgac tgttttgctc cactctttca gtatcgcaag caactggtta
     8041 tacgctgcaa tgctaccata tcgttcttcg cttttataat gttggtacat caaatagatt
     8101 tggtattgtt tttgattcaa caaaactgct agacaggcat tctcgtcttg acgagattcg
     8161 cttcggtacg cgctccaaaa atgactgcgt aaattccagc cattcgtcca gctttcgatt
     8221 ttaggtttgc tcatgccgta catatcaggc aaaagtgcag ctgtctggag ctgaatctct
     8281 ttccattttt gccagtgttc tttatattct ttcttgattt gttcaatctt atctgttgga
     8341 tggctctctt tgagctgttt gaatgcgtag attggtcgat caaacaactg ataatctgat
     8401 tttccaagtt ccattgtttc gcctctttcc gttatctttt gtattctacc tgaattttgc
     8461 gatatggaca aatagaggtt gtgaaaaagc tgttctgcat caagtcataa gaacagccaa
     8521 acaaaaaact gcgatgagca tcaccgcagt tttattgaac ttaaggttga ttgatcacat
     8581 ctaaaagcgt tgaaaataaa atggcactag aagcagctcc tgggtcgata tgtccgattg
     8641 cacgctctcc tagataggaa gctcgtcctt ttttagctac caagactttg gtgtgttctt
     8701 ttgcctggtc aatgatttct tttgttaaat tccctgcctt caatgcctct gtgactggca
     8761 gccaaacatc taacatcgtt ttatcgtcag cttctgcctt tcctcggctt tgaatacccg
     8821 ctgttccctg ctcgatcact tcgcctaatt tttcttgaga atcaatagaa tcgacctctt
     8881 ttgtcgcttt catcatgttc atgaatgcag atccgtataa cggaccagaa gccccaccga
     8941 ctttcgatat catcgccata gagaatactt tgaatgtatc gctgattgtt tcaggatgat
     9001 cggcttgaaa agcttcttca taagctgaaa cccctcttcc catgttattt ccatggtctc
     9061 catcgccaat cggggtatct agatcactga gatattgttt gttctgattg attgcctctg
     9121 caaattgcgt gagccattct tggatttctt gaacggttag tttcatatta tcaccctttt
     9181 atcttgagat tttttaccat gaaatcgtct gtacattgct ttctaaagaa tctttccatt
     9241 gtgtctccgt caagtcgatc agtgttagag aaagaccttg catatctagt gaggtcataa
     9301 aattaccgac ttttcggaaa actacattga cgcctctttc ttccaacaga gctaaaacat
     9361 cattcatgaa cacgaactgt tccatcaaag gcgtcccgcc cattccgttg actagtacac
     9421 ctgcttcttt tggtaaattc gagtaatcat ctaaaatctt agtaacaagt tcctttgcca
     9481 gtactttaga aggctgcatc ttttctcgac gatagcctgg ctcaccgtgg ataccaacac
     9541 catactcgat ctcatcttct cctagttcga atcctggttt ccctacttct ggtacagttg
     9601 ctgctcgaag cgctagaccg atcgtcttag ttgcctgtac gaccttttct cccagttctt
     9661 tcaactcagc tagggaagca ccgtttcttg ctgcatcgcc cacgattttg tgtacaagga
     9721 cagtacctgc aactccgcgt tttccagcag tatacgtact attttcaacg gcgatatcat
     9781 catctacgat cacactttct acttcaatat cgtccatagc agctaattcc tttgccatat
     9841 cgaagttcaa tgcatcccct gtataattct taacgatcag caaaactcct tttccactat
     9901 cggcttcctt aatagccgtc tgaatctgat ctggagttgg agaagtaaac acatcaccaa
     9961 gcaccgctgc acttaacatc ccttccccta caaacccggc atgggatggt tcatgtccgc
    10021 ttcctccacc agaaaccaaa ccaacttgtt ccgctttctg attcttggct acaacacggg
    10081 aagattctac acgatgaaca agttctggat agctttttac taatccttcc agcatttctt
    10141 cgacaatatt gcttggatca ttcatgattt ttttcaattt attcccatcc tttctttccg
    10201 gcattttgtg cttctgttaa gatttgctga gtatcatctg ttataccggc agtgatggct
    10261 gctgcaaaag ctccttccac taaaggcgca tccaccagat gataacgttg ctgctgctct
    10321 tcttccaaca tgtcttttgc cagttccgag ttcaataccg cgctaccgat atccgcaaaa
    10381 acaaaataac tagctgcatc gcttgattca ttgactgctt ctacgatttt catcgggtct
    10441 gatccgatct cgccatttat tccgccgaga gaaaaaattt gcacctcttc ggatttttgc
    10501 atctgctcaa tcatttcttt aataccatct gttacttttt gactatgaga aataagcaaa
    10561 atactttttt tcacaagaat cttcctttct tttacatagt tgctaacgat tggtttcgct
    10621 ttcattaatg aacaattcaa gtatataata aatttctaat tttgggaaaa tatgtgtagt
    10681 gcttcttgaa gtttgttata ctttaattaa aagagaggag tgatcgaatg attacaacaa
    10741 caacgaacag tgtagaggga aaagccgtag tagcttataa agggatcgtt tttggtgagg
    10801 tcattactgg gatccatgca tttaaagact tggaggcggg gttgcgaaat atctttggcg
    10861 gccgctcaaa aagctatgaa aacgaactaa tgggtgcaag agaagaagca cttgccgaaa
    10921 tggccgaacg tgcaaaagca ttaggagctg atgcgatcat tggcgtaaaa atggactatg
    10981 aagtattagg cagcgacaat ggcatgctga tggtcacatg tagtggaaca gctgtctcca
    11041 cgagataagt aggggacatc tgttttaata actaaagaga gatgggacat gtagtggcac
    11101 tcaagaatta gatttttcag tctaacttcg aggtactcta ccttcccatc ctctcttttt
    11161 tatttctttt tgcggatgat tccgtaagct gctgcgacta acacacccgc ccctgctaag
    11221 gaataactga cagaagctgt tttttcattt gtttttggca aagtctgact tgttgatttg
    11281 gatggctctg tcgtttgctg aactgtttct gtcgtgcttc cagaggtcac tgatttttct
    11341 cctgccgaat gcaaaatatc ttttattcgt tggaaactag ctgctaagtc agcttttttt
    11401 gcttcaaaat cattagcaac tgtttgtgct tcgctgatct ttccgtggat tgcttgactg
    11461 gccgctgtga tatccgcttg agtttgactt agctttccag aagctgtttg agcaacctga
    11521 tttaattgct gattgaattc ttttgcttgt tggatcttcc cgtcaagatc tgtttggaca
    11581 gcttccgctt ttgctttcgc ttcggcaagc tttttctcaa gttgtgcacg gtcatttccc
    11641 actttatttt taagatcagc taaatcttga tcgattgctg cttggtcttc tttcaatttg
    11701 ttttttgcag cttctaatgc ttcttcgagt tcttttcttt tattttccaa gtcttgcaaa
    11761 tgatcgattt ggtcagaaga ggtagaacta tttattgatg tatcttctga agatgaaatc
    11821 gtttctcctg aattatctgt cggtacattg gaagtcattt ctgtttccga ggcaccgttt
    11881 tcagcaccag aagagcttat ggttgttgaa ggttctgttt gagcaggttc agaagtttcc
    11941 gcaaaagctg tctgtcccaa taagaaaata ctggaaacag caattggcca gattaacggc
    12001 ttcattcttt ttttcatcac ttttcccatc cttactactt tttaactaat ttcttattat
    12061 aagcaaaaat gtttggaaga accataaaca caacaggctg tttgtctaaa aaacggtgca
    12121 tcttttattt ttttgtacgt tccgggcttt tccatgtccc gcttccataa cctaaaatcg
    12181 ttttgactgc tggaatgaaa gtcgtcacga tcgttatcgc gttcactatc cagaacaaaa
    12241 gcatatatag tggtgcgaaa agaaggtatt tcaattttct tcgtcgatca tctacaatca
    12301 gcgaagctag caactggaaa aatcctgcga ccatttcaaa gcagatgaac acaaaactga
    12361 atgtaaaagt aatataaatc tgttcgtagt ttccttgata tacataatgc ccgattaatc
    12421 cagcaaaaag aactacggaa atacaaaaga atattgacca gacaatactc aatgtttgat
    12481 caatgaagat gatcgttctt ccaatatgtt taaaaggatg gagcatcact tttttgaaat
    12541 tcgtcagcca gacttctgtt ccaccttttg cccaacgttt tctgtgtcga tatagcatct
    12601 tcaacgtcac aggtacttcc atgaaaaaga taatctcagg agcaaaaaca gataaccagt
    12661 catttaactg atgatcccaa gcaatactga tgtcttctgt cgctcgatct tgtctgaatc
    12721 cgcccacatc gatcaaggct tcttttcgat acatcgtatt tgctccactg tacgcataaa
    12781 gaccaccgaa aacagctgac tgtgttcgtt taatgatgcc cacgatactt gagaattcaa
    12841 ccgtttgaga ttttgcaatc aatttggtac gattttggac atccatattt gcagtgaccg
    12901 cagcaatatg tcttgcacca gggcgaatga aataattcac atacctaatc agtgcatctg
    12961 gctccggtac cgtgtctgca tcattgctta aaatcaattt cccttttgca aaggcaagtc
    13021 cgatattaaa cgcgtgggct tttcctttat ttttttcgat ccgaaccaca cgaagattag
    13081 cgtatttttt catcagtcta gctaatattt ctggtgtttg gtccgttgaa ccatcgtctg
    13141 tcactaatac ctcatagttg tgataattca atttcgtcat caaatactcg attgtatatt
    13201 cgatcacaat ttcttcatta tgggctggca ccatgatcgt gatgaacggt tcgacactca
    13261 agggaacatc tacccattct ttttgcttat gtttgtataa aaacacataa caccagacac
    13321 caataaacca tacaaatccg cctaaaatcg gataagaaac gagaaaaaaa tacataatag
    13381 acaaacaagt atctaagaat aaattacctg tatgggtgat tgagatcatt tcagttccac
    13441 cccattttct ttatacaaat ctgcaatgaa tgtcgtatct agatttttct cttcggagac
    13501 agaataaaaa cggaccgttt ctctctcttc ttttgttcca aaacgttccg tatacacttc
    13561 ttcaagtaat tcttgtcttt ttttcaattt ttcatcttca tattcaaaag atttatttac
    13621 ttttttgtat agatttcgat tgttccacct agtcaataca aatgaggtaa tcaggaaact
    13681 gaaaaaaatc aatacaaaaa agccgattaa gaaatacagc attttctttt ctgataaaaa
    13741 agcgtacaag gaaaaatcct tggctctttc tggtctcgtt aatgggaaaa agacccatat
    13801 aaaaggaaga acaacgatca tccatcctaa aagagccact aacgtttgcc aaatctttaa
    13861 ccagtaatta ccttttgaaa aatacttatc tttcaccaaa attgatgcaa cttcttctct
    13921 tgttactttt ttcatatcct cacctcacgt ttttttgtta cgacgtagga gtttaccata
    13981 catttcattt cttttcacat aaagtgttcc taaatttttc ctaagaaaaa gaacaaaaca
    14041 tttctcatca gcaaatgaaa aatattttgt tctttttatc agttgttctt tttatttttt
    14101 atctcttaat tatgttgctc tttccaatac gacaatgctt ttcattcctc tagatatcgc
    14161 cgtgtacatt tgtctgcgtc ttctttgctc atctccatcg ttttcaaatg gatataagat
    14221 tacatgatca aattccagtc cttttgctag actcaaagga atgatttggc agttttctaa
    14281 tccatatgaa ttttttttaa tcgattcttg tagcgtttgg gtttcctctt ctgtttgggt
    14341 gataactgcc gctttttctc cttttagaga aaccaaaatc tcagctagta ttttcaacaa
    14401 ttcttcagag gatcgaaaag agcggtattc gggttctttt ccttgcggtc ggatcgatac
    14461 gatgtcgatc ttctcttgat ccacagcata ggttttgaaa agctctgtga ttgctccgct
    14521 agaacggtaa ctatttctaa gatcataccg atggatcggc agatggtagt cgtcgaaaca
    14581 acgcataata tcagcaaaag tagtggaaga attaaagatt gcctgattct cgtcacctac
    14641 taaagtaaag tgagtttttg gaaataattc gatcaataga cctaattgtg ccagggtata
    14701 atcttgtact tcatcaatca atagatagcg aaatgctggt acatttacct tttcaacaag
    14761 taaatgacga atagctaata taatgactgc ttcatcaaca gtacgtgtgg gttgccaagc
    14821 ataagctgag ccatatcgtt tttcataaat tctttcaaac aactgatgct catccaccca
    14881 tgccatttct tcgatttgtc tggttatttt tcgatatttt ttttgtaaaa gtttccttgc
    14941 ataagctaca atactttgtt ccgaatcatc agaaatcaat ttaccgaata attccaactg
    15001 cctatcttct gtcaacgaga gtacctgatt ctgcacagca ggactttttg cttgtttcaa
    15061 aagatgattc ttccattcgc tcatcagtgc ctttttcgtt gcttgaagcc tatcgatcaa
    15121 tttaggatga gcaggaactt tctcatatat tttttcgatc gtttttttag aaaggactgt
    15181 ttttccattt ttagtaagtc cacggataaa attcggatga tctaaaaaca aaggatctga
    15241 ttgttttaga ttatcgataa actttttgct acgcaaacgt tctgtctgct ccgatacatt
    15301 ttctcccgtg atacgtttga aataagcctc ttcaccttct atttcctctg ctgaaagctg
    15361 tgaaaccagc tgtatgatgg ttagattcag tggatttctt tcacctaatg atggtaggac
    15421 attcgaaata tagtcgataa accgatgatt aggtgataga atcagcaagt catcggatgt
    15481 catcgtatct cgatataaat agagcagata cgcaatacgc tgcatgatag cagaggtttt
    15541 accacttcct gctacgccat ttaccaagat attttgactt ttcacatccc gaatgattgt
    15601 gttttgttcc gattggatcg tagcagtgat agccttcatt tcattggtct catcttggcc
    15661 taagacttct aataatacgt catcttgaat agctacagac gtatcaaaaa acttgaccaa
    15721 acgatctctt tccaaaagaa attgtcgtct ttctttaata accgtatcga ttttattttg
    15781 acgaaccgta taagaagatg gaccaatcat gttattgtaa aaaagttcgg caattggtga
    15841 acgccaatca taaacaagat tttcatctgc atcatctgtg aaatttgctg taccgatata
    15901 aaaggattct tttggctcat tatccagaaa gtctacaacg acttttccaa agtagggact
    15961 tttcaataaa cgttcgatct ttgctagctg attttctgcg tttttctgtt tgatattcat
    16021 ctgatcgatt tctctatttt tcatttcaat catcgaaaaa gtatccaggt tatctaagta
    16081 actgtcaaaa ttcaatcgaa gctcgccagc catttctttc aacgactggt ttccttctat
    16141 ttttgccgta tccatcgatt cccgcagttc gtttttttta tcaattagct tttgatacac
    16201 gttcttcaga tatgcttctt ctaactgttt ttgtgtgttg accatacaca ttctcctctt
    16261 tttttaaagt ggtggaaccg attagtttac ctcttttaag agtcgaatgc aagcttttta
    16321 acgagtatca tttgttagga tatatcagtt cttcaggtcg gacagtttga tataaagaaa
    16381 atgcaccatc aaggtttttt acgtggtagc cagactgctt caagattctt tccgctagat
    16441 agctacgtaa tccgctatga cagctgacga tatattcttg ttggctgtct aattcattta
    16501 atcggtcccg aagttcattc aaagggatat gtttggcttc cttgaatttg cccttttcca
    16561 attcttctgc tgttcgcaca tctaataaaa tttttccttt tgctagttct gtcggcaatt
    16621 catgccattg tacggtttca ctaagccctt ctgcaatgtt cattgctgca tatcctagca
    16681 tgtttactgg atctttggct gagccaaatg gcggtgcata cgtcaattct aattccggta
    16741 aatcgaagat cgtcaaatgc cctttgattg ctgttgctaa aatatcgatc cgtttatcca
    16801 caccttttgc cccaacacct tgtgcaccat aaatctcgcc agtctctgga tggaagatca
    16861 acttcagtaa aatatctgtt gcttctggat aatagcttgc atgatctttt cctgatacat
    16921 gaatgacttc aaaagacagt tcgttttctt tggctgttct ttcacttaaa ccggtggatg
    16981 cggcagtcat tccaaacgtt cgtataattg ctgttccaat accgccttga tttcttcgag
    17041 aaattccgga aatcgtgtcc gcaacttgtc taccttgacg attggctggt gaagcaagag
    17101 aaataagtgc atcttttcca gtgatttgct gttttacaac gatagcatcc cccaccgcat
    17161 aaatatcagg aatattcgtt tgataacgtt cgtctactag gatacccccg cgtatgccta
    17221 attcgattcc agcggtcact gctagctggt tatcaggacg aacaccaatg gataaaataa
    17281 ctaaatcagc agggatggta cgcccatcat ccagtcgcaa tcgtttcccg tggtcttcaa
    17341 atccagcgac agcatgagaa gtaatcactt ctactccttg atgagacaat tctttttcta
    17401 taaaagctgc catttcttca tccagtgttg gaagaatatg tggtgcttgt tcgatcacca
    17461 tgacttctaa acctcttctt tttaggtttt ctgccatttc caatccgatg aatcctgcac
    17521 caacgatcac ggcacgcttt ggctgttttt ctaatgcatg catcacttta tcgatatctg
    17581 gtacatttct aatagaaaaa acattgtcgg cttctgttat ccctggaatc gatggaacaa
    17641 ctggttttgc accaggagat aaaaccaatg catcgtactg ttcaatgtgt ttttgaccct
    17701 catgtacgac ttctaccgtt ttgttttcgg gaaagatggc tgtcacttca tgatttggac
    17761 gcacatctaa acaaaaacga gcggataatg attctggcgt ttggacaaga agattttccc
    17821 gttcactgat ttctcccgaa agataataag gcaagccgca atttgcaaaa gaaacatacg
    17881 gtcctttttc aaaaacaacg atttctgcat cttccattaa tcggcgaagc cgtgtagcag
    17941 cagacattcc acctgctaca cctccgacaa taacgatttt catcttattt ccctcctact
    18001 gttcgatcaa accattgatt catgccacca cgtacattca ctacatcata accagatttc
    18061 tttaattctt tagctgcttg tttactcctc atccctgatt gacaaatcac atagacggtc
    18121 ttttctttgt ctccatcata tctgtttata ctttgtaatg gaacattttt agctcctttt
    18181 atatgcccgc cccggtattc tgcaggtgtt ctaacatcaa gtaacgctgg attttccaaa
    18241 agcttttcct ttaattctgg gactgttatc gattttattg attgaaatag caaattcatt
    18301 ttctgcacct catttacatt tttataaata cctcatgggg tatattaaac aggagcttta
    18361 ttaatatgtc aaccagaatg actcttcatt tttttcacta ctttttatat ttgcataaaa
    18421 taccgataag gggtattatc taattataaa aatagaggag aatgtgttat ggagattgat
    18481 aataaaaata tcctgaaccg tttgaaacgt acagagggac aaattcgtgg tatccaaaaa
    18541 atgatcgaag acgaaaaaga atgtatggat gtcatcactc aattaagcgc tgttcgttct
    18601 agtattgatc gcgtaatggg catgatcgtt gcagataatc tgaaaaattg ctttgagaaa
    18661 cctgatagta atccagaaga acaagcggct aaactagaac aagcgatcaa gatgattatt
    18721 aaaaagtaaa ggaatgattt caatgaaagt cgttttccat attgatgaat tagaaaaatg
    18781 gtcagaaaca gggaaaaacg taaaaaacct gataaaagct tctccagaaa cgacgattgt
    18841 cgtatctgta aatggcatcg cgattaccgg ttatctagac tctgccaatg cagaattctt
    18901 agatttgcaa ggagtcgttt ttcacgcttg cgccaatgct atgcgtgcta atcatatttc
    18961 agaaagttct ttacctgaac aagtaatcgt tgtaccagca ggtgtccttg atttagttga
    19021 actgcaatcc caaggttatg cttatatcaa gccttagtca gtagaggttg cgacagaagt
    19081 gatcagcttc aagaaatttc ggcttatttt tcgaagaagc tacttctgga aaaccgtgaa
    19141 tcaaaggttg tgaaaacgct gtcctgcatc aagtaataag aacagccaaa caaaaatagc
    19201 ttctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    19261 taaggaagag gttgtgaaat cagcggtttg acctaagtat tagagaaaaa aacgggaaaa
    19321 tagctcctca tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg
    19381 tttaaggaag agattgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
    19441 aatggatttc tacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaata
    19501 ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg agtattagag aaacaaacgg
    19561 aaaaatagct gctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa
    19621 caccgtttac caagaggttg tgagcctacc atttaactcc gaatcacaag aaataatttc
    19681 aaaaacaact tctcctgttt ttggttcggt tggcttatta tcgagaagta gctactggga
    19741 gtctatttat caaaaaaagg aatgcgacat aggtcacacc ccttttttat tttctttgaa
    19801 acatctttcg gtactcgaca gcatacaaga caaaagattg taaaagaccg taagtgatga
    19861 cggcaaaaat gactctcacc cacagcgttg gtaattctgg gaaaagcagc aaaaggatca
    19921 tgccaacata gatgacagca gtcgctactt tgccatacca cttcgctcca cttaatttga
    19981 tatttttaga tagcccgatt aatccagcta caaacataaa gccttctttt atcaagaata
    20041 gaccaaacaa cagccaaagc cacggtcgat accatgccat agacaaaatc agaaaaagtt
    20101 gtgtcaattt atctgcaaac ggatcaagca ccttccctaa gtctgttacc tgattatatc
    20161 tccgagcaat gaccccgtct agataatcag acaaacctga gacaacaatg accgctcctg
    20221 ctatataaaa tgactgtctc agcacaaaat aaagataaat cggcagcaaa aaaatacgga
    20281 atatcgataa aagattcgga atcgtttgta tttctttacg ccagtctgtg cttaatttca
    20341 ttttatcgcc ctacctcctc agcactcttt tctttttatt ttagcttaca cggggaataa
    20401 aaccaatgat ttcactgttt ttatttttct ttacagacac tgatattatg agctaatttg
    20461 agataatcac ttgctgtttt catcgtttac tcttctttct tcctgtcgtt tgcgcatttc
    20521 ttttactagt actttatgag ctttttttga tcctcgctct aggtcacgct gccgtttacg
    20581 tgttttataa tcgataaaca gactgtctaa atcgtaacct gtcgggtaca gatcttctgc
    20641 tgaacgcacc agacgaacac gctgacgagg cacgtggatt atttttttgt ctaaatatac
    20701 atcaatcgta tcttcaccta tatcttcata aactaacgct tctttttggt attcattgac
    20761 aaatatccga tcaccttttg caaagagctg acgttctttt ttattctcct tcctatttgt
    20821 aactgctgtc ttaggaagaa attctttttt ctgtgtccga taggcttttt ccgccaagta
    20881 acgctctgct tgatggatca atttcatcga catatccatt ttatgagcaa tccacaaagc
    20941 ttggctttct cctgtttctt ccaacccgca actgatattt aggacgaagt gcttcccgat
    21001 cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc ttgatttctc
    21061 cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc atgattgcga
    21121 tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct aaaagtacca
    21181 acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg
    21241 tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc tcatcaaaga
    21301 cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc atcaaagtca
    21361 ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg gtgataatca
    21421 agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca ggtaaaaatg
    21481 gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt gtgcttccat
    21541 tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg atcgtttcga
    21601 tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc gctaggactt
    21661 gatattcttc actgatttct tcagctttta acaactgata gtgttcattc aatttagaaa
    21721 tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata atcgttccat
    21781 ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct ttttgaacaa
    21841 tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc agtaattttg
    21901 actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg
    21961 cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct acatttatca
    22021 aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga ttcttatcaa
    22081 aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc aaatcggttg
    22141 gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca
    22201 taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac
    22261 gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt tttttgctat
    22321 agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt aattgactaa
    22381 tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca aaataaataa
    22441 gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc ctatccttat
    22501 tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac caaataaact
    22561 ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa aacatacccc
    22621 attcttctag gtcttattta ttttccactt aactatagca agacacattt tggttgtcaa
    22681 actttagtat gttttataga tttacaccat taatgaatag acacctaccg cgataattgc
    22741 tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag ttcctttatt
    22801 agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag ggttgattgc
    22861 atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac ctacagcgaa
    22921 tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa gaactaatac
    22981 aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag caggacctgt
    23041 tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg gcaaataaga
    23101 aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac
    23161 gacaagattc caactaattg cccctgtcat cgccatagct agagagactg caggattcaa
    23221 atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga gtccccaacc
    23281 aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat tcacagccgc
    23341 acacacacca tctccaagca aaactaggat cagtgtccca ataaactccc ccatgatttg
    23401 agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg attctgcaat
    23461 caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt caaaatatcc
    23521 agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt caaacaacag
    23581 atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt cttctaaacc
    23641 ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca tttcttttgc
    23701 taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag tagcatcttc
    23761 ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt cttctaattt
    23821 agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt ctttcgtctc
    23881 tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt ctgccatctt
    23941 acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt ctctttctaa
    24001 agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt ccaaatggtt
    24061 caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac ttactgtatc
    24121 gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat tgtaatcaga
    24181 accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga tatcattcaa
    24241 tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat ctacatcttc
    24301 ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg taccaaagta
    24361 agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc ctgtatcgaa
    24421 gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga tccctttagt
    24481 tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt tatctaccca
    24541 aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat ctgtcagcag
    24601 gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa ccgctttcat
    24661 tttacttact agataagccc catcttcggc agcctttttg atattatcaa tcactaaacg
    24721 ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt cttttttcaa
    24781 aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat atttggttcc
    24841 tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga acatattgaa
    24901 ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt ttgggatgtg
    24961 tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta cttccacatc
    25021 aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag aggaagtccc
    25081 ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg ctgactggac
    25141 tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt cttcttgctg
    25201 cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt cctccttatt
    25261 caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt tctcggcgct
    25321 cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg atctcgtctg
    25381 tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt
    25441 ccaagttttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat tgcatcagat
    25501 attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt ccagtgtcat
    25561 cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa gtagctttaa
    25621 tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt gctgcctgat
    25681 cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca tcttcgcttg
    25741 tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga agtccatctc
    25801 tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca tagtaaacct
    25861 ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat tgcggttttt
    25921 caccggtatt catgacaata aatgaacctg taccataagt attcttgacc atgcctggtt
    25981 cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct gaaattggtg
    26041 cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag ttagaagtga
    26101 cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga tcccaatcta
    26161 gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta acatgtgctt
    26221 gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc aattcgcctt
    26281 tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc ttcgttgctg
    26341 agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga tagccttcat
    26401 tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact gcatgataga
    26461 ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc gttatgccaa
    26521 tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca ataactgact
    26581 gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc gggaaatatt
    26641 gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag ataattgctc
    26701 gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc attgtgattc
    26761 cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt tttaaaaaaa
    26821 gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt atgaagctat
    26881 tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat cgaataatgt
    26941 cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt ctctggcaaa
    27001 atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca ataccgccga
    27061 ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc ggcagacttt
    27121 ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc aaaagaacat
    27181 tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc ctcggcaatt
    27241 gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc ccgagcattt
    27301 tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca acaaagaaaa
    27361 accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct ttgattttca
    27421 aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct aaaacagcgg
    27481 ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg tctactatcg
    27541 aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta tacattcagt tctgctgctg
    27601 ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg tccgactgat
    27661 agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat agtctttttt
    27721 tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac gttgtttcta
    27781 atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg
    27841 gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt atctgtagct
    27901 cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag agctggttca
    27961 attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc gctaatcgaa
    28021 cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc aagctttcct
    28081 gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca agcagcgcct
    28141 gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc aattcaatat
    28201 cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc aaaccattga
    28261 tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt aactgctctt
    28321 ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga ccacaccata
    28381 tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc
    28441 catccttctt ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt
    28501 tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag tttaactttg
    28561 ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt ttggggataa
    28621 aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa ccactgattg
    28681 ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt cttgctgaca
    28741 tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc atttgttgga
    28801 cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga cgtttgcaag
    28861 tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc ccacatctga
    28921 ttgtcgggac gagaatgatt agccagcaaa acaagatcat agttctcctt tcttttttcc
    28981 gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc taaaaaaagt
    29041 gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg atctagataa
    29101 cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct tgatgccaac
    29161 ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc
    29221 atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg aataagcgtt
    29281 gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat caaacgatca
    29341 aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc tattactgtc
    29401 ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa
    29461 agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct gggaatccat
    29521 cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa ttggcgaaga
    29581 taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt
    29641 cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca gtattcataa
    29701 ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc catcataccg
    29761 tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt atcatttctg
    29821 aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt tacgtacctc
    29881 tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag
    29941 ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg cttcgcctaa
    30001 ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt gaaccacttc
    30061 tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat cttctgctaa
    30121 gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga acccagtcac
    30181 tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca atcctacaac
    30241 accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag cgactttccc
    30301 aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat aataaaccgc
    30361 tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg cccgatttct
    30421 gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa cactttgcgg
    30481 ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc cattttttac
    30541 taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac gctcgatctt
    30601 ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat cctctttcta
    30661 taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct tggcaaataa
    30721 agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg gttttccaat
    30781 aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc aagttctgca
    30841 tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc aactaaaggg
    30901 atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt gtatttatca
    30961 gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag ttttgactca
    31021 cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt ttcatattct
    31081 tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc aaccgctgag
    31141 aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct accgccccat
    31201 tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata cgtatttgct
    31261 ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat
    31321 gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc cgcatcagga
    31381 cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt tgctcgactt
    31441 gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc ttggattgct
    31501 ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag ttcacctggc
    31561 cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc aagaccttta
    31621 gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa aaagctgagt
    31681 tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga aggtaaagta
    31741 attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga ttaacctatc
    31801 gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt tgcttaaaaa
    31861 attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta attctttact
    31921 tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc taccatcgac
    31981 ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc gaccttcgct
    32041 aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa tcgttgatgg
    32101 ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt cttttaatat
    32161 caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt catcagctaa
    32221 tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat ctaacgcttt
    32281 ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt atgattttta
    32341 ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg aaaaaaagaa
    32401 gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga
    32461 atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg
    32521 ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt gaacaccgtt
    32581 tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc caaacaaaaa
    32641 tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga ttgtacaccg
    32701 tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca atttcaaaaa
    32761 acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc aggagaatac
    32821 tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt atttcctttt
    32881 attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat gattttttct
    32941 ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata tatccataac
    33001 gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac gacgtataac
    33061 caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc tgcttcaaat
    33121 agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct atagctccga
    33181 gtccgttttc agctatgaac agtggttttt gatacctatc atataattga ttcaaagcaa
    33241 ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga tacggatttt
    33301 taattcctcc agttattaag tttccttgga ccccttccca attttctgga tgagcactga
    33361 ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct gctaagattt
    33421 cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt cgttgcgtat
    33481 acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt ctttgctgct
    33541 caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc tgatagctga
    33601 tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta gctaaagcac
    33661 ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg ggatggtttt
    33721 cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc tcattgaaag
    33781 tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg aaacgaacgt
    33841 aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa tataacggca
    33901 tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat tcgtcaaaca
    33961 cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa
    34021 ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca aataaacgga
    34081 tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa taatacttgc
    34141 tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat tgatctgtcc
    34201 atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat aatccttttt
    34261 catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc cataaaaatc
    34321 cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg tttgattatt
    34381 tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa taaccattgt
    34441 ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca ataaatcgcc
    34501 tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat ttaaattcac
    34561 tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta ccccaattgc
    34621 atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca gaactgtaga tttttccgtc
    34681 cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg gatcgtttac
    34741 ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat cctttgagga
    34801 ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg aactgatcat
    34861 cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa tcgggaaaga
    34921 caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag ctactccacc
    34981 aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa gagtcacacc
    35041 ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata aagataaact
    35101 ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg cttgcgcagc
    35161 tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa gattgttggt
    35221 cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc
    35281 gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc cagctgcaat
    35341 cagctgactt gcataatctg caattggacc tacaactaat aaaataattg ggaccatcac
    35401 gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat
    35461 cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa ccgtacttgt
    35521 ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat cactatatgc
    35581 actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga aacgatctgt
    35641 attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga aaaatgcatc
    35701 agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat tcaaactcgt
    35761 acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta aagtcggggc
    35821 aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct cagttcctct
    35881 ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt catacacttc
    35941 atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta cagtgatcac
    36001 accttctagt tgctctacct tctccttatc tgccttctta ttatctttta gggtaaagcg
    36061 taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga caaatcggac
    36121 aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa ttaatgccgt
    36181 agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca ttcaattagt
    36241 accccttcta atagcgaacg aaactgtaca atagtgtgta tggtttactt agccgagcaa
    36301 atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa aggagacaaa
    36361 aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct gatactctgt
    36421 gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg catttaacgg
    36481 aagcataact aggtcagaac taggagagat tagttgctga atggaatcta tctttttatg
    36541 gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt tctttttttc
    36601 ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt caaactcatc
    36661 tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt gctcagtagt
    36721 caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat acactggttc
    36781 tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt ttagtgtatt
    36841 caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc gaaaaggatt
    36901 caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg gacgtaattg
    36961 aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac gggagatgat
    37021 cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt tcatttgttt
    37081 gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa
    37141 acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt ttttttcatc
    37201 attaatcatc aaataattta actctaatag tcgattttca attgcttgtt tttcttgatt
    37261 ggaaaaagaa tagggt
//
LOCUS       DAZUMV010000016        34397 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000016 DAZUMV010000000
VERSION     DAZUMV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.016"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8FK_000712"
     CDS             352..1041
                     /locus_tag="ACG8FK_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5096343.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8FK_000713"
     CDS             1028..2236
                     /locus_tag="ACG8FK_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5096344.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8FK_000714"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8FK_000714"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS5096345.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8FK_000715"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8FK_000715"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS5096346.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG8FK_000716"
     CDS             complement(6339..7034)
                     /locus_tag="ACG8FK_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS5096347.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8FK_000717"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8FK_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS5096348.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG8FK_000718"
     CDS             complement(8233..8424)
                     /locus_tag="ACG8FK_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS5096349.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG8FK_000719"
     CDS             complement(8445..9335)
                     /locus_tag="ACG8FK_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS5096350.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG8FK_000720"
     CDS             complement(9322..10089)
                     /locus_tag="ACG8FK_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5096351.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8FK_000721"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8FK_000721"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS5096352.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8FK_000722"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8FK_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS5096353.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8FK_000723"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8FK_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS5096354.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG8FK_000724"
     CDS             14660..15043
                     /locus_tag="ACG8FK_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096355.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8FK_000725"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8FK_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS5096356.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG8FK_000726"
     CDS             complement(15987..16805)
                     /locus_tag="ACG8FK_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS5096357.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8FK_000727"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8FK_000727"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS5096358.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8FK_000728"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8FK_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS5096359.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8FK_000729"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8FK_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS5096360.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8FK_000730"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8FK_000730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS5096361.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8FK_000731"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8FK_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS5096362.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACG8FK_000732"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACG8FK_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS5096363.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8FK_000733"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8FK_000733"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS5096364.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8FK_000734"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8FK_000734"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS5096365.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8FK_000735"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8FK_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS5096366.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8FK_000736"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8FK_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5096367.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8FK_000737"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8FK_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS5096368.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACG8FK_000738"
     CDS             28113..30086
                     /locus_tag="ACG8FK_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS5096369.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACG8FK_000739"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACG8FK_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS5096370.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG8FK_000740"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG8FK_000740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS5096371.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACG8FK_000741"
     CDS             32965..34257
                     /locus_tag="ACG8FK_000741"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS5096372.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10495 a         6692 c         6807 g        10403 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25201 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25261 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26641 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca
    34381 cagtcccttt ttccgaa
//
LOCUS       DAZUMV010000017        33285 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000017 DAZUMV010000000
VERSION     DAZUMV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.017"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..406
                     /locus_tag="ACG8FK_000742"
     CDS             131..406
                     /locus_tag="ACG8FK_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS5096373.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            412..1179
                     /locus_tag="ACG8FK_000743"
     CDS             412..1179
                     /locus_tag="ACG8FK_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS5096374.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1347..3176
                     /gene="typA"
                     /locus_tag="ACG8FK_000744"
     CDS             1347..3176
                     /gene="typA"
                     /locus_tag="ACG8FK_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS5096375.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3437..5209
                     /gene="recQ"
                     /locus_tag="ACG8FK_000745"
     CDS             3437..5209
                     /gene="recQ"
                     /locus_tag="ACG8FK_000745"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS5096376.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5323..5625
                     /locus_tag="ACG8FK_000746"
     CDS             5323..5625
                     /locus_tag="ACG8FK_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS5096377.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5628..6791
                     /locus_tag="ACG8FK_000747"
     CDS             5628..6791
                     /locus_tag="ACG8FK_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5096378.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6817..10245
                     /locus_tag="ACG8FK_000748"
     CDS             6817..10245
                     /locus_tag="ACG8FK_000748"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS5096379.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10266..11390
                     /locus_tag="ACG8FK_000749"
     CDS             10266..11390
                     /locus_tag="ACG8FK_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS5096380.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11371..11817
                     /locus_tag="ACG8FK_000750"
     CDS             11371..11817
                     /locus_tag="ACG8FK_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5096381.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11801..12139
                     /locus_tag="ACG8FK_000751"
     CDS             11801..12139
                     /locus_tag="ACG8FK_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS5096382.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12258..12818
                     /gene="rsmD"
                     /locus_tag="ACG8FK_000752"
     CDS             12258..12818
                     /gene="rsmD"
                     /locus_tag="ACG8FK_000752"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS5096383.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12815..13306
                     /gene="coaD"
                     /locus_tag="ACG8FK_000753"
     CDS             12815..13306
                     /gene="coaD"
                     /locus_tag="ACG8FK_000753"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS5096384.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13296..14339
                     /locus_tag="ACG8FK_000754"
     CDS             13296..14339
                     /locus_tag="ACG8FK_000754"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS5096385.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14498..15226
                     /locus_tag="ACG8FK_000755"
     CDS             14498..15226
                     /locus_tag="ACG8FK_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS5096386.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15342..15587
                     /locus_tag="ACG8FK_000756"
     CDS             15342..15587
                     /locus_tag="ACG8FK_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5096387.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15584..15802
                     /locus_tag="ACG8FK_000757"
     CDS             15584..15802
                     /locus_tag="ACG8FK_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS5096388.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15938..16477
                     /locus_tag="ACG8FK_000758"
     CDS             15938..16477
                     /locus_tag="ACG8FK_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS5096389.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16470..18743
                     /locus_tag="ACG8FK_000759"
     CDS             16470..18743
                     /locus_tag="ACG8FK_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS5096390.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18781..19827
                     /gene="holA"
                     /locus_tag="ACG8FK_000760"
     CDS             18781..19827
                     /gene="holA"
                     /locus_tag="ACG8FK_000760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS5096391.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19916..20170)
                     /gene="rpsT"
                     /locus_tag="ACG8FK_000761"
     CDS             complement(19916..20170)
                     /gene="rpsT"
                     /locus_tag="ACG8FK_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS5096392.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20570..20839)
                     /locus_tag="ACG8FK_000762"
     CDS             complement(20570..20839)
                     /locus_tag="ACG8FK_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096393.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21101..21229)
                     /locus_tag="ACG8FK_000763"
     CDS             complement(21101..21229)
                     /locus_tag="ACG8FK_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096394.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(21309..21764)
                     /locus_tag="ACG8FK_000764"
     CDS             complement(21309..21764)
                     /locus_tag="ACG8FK_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS5096395.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21902..23830)
                     /locus_tag="ACG8FK_000765"
     CDS             complement(21902..23830)
                     /locus_tag="ACG8FK_000765"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5096396.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(24027..24860)
                     /locus_tag="ACG8FK_000766"
     CDS             complement(24027..24860)
                     /locus_tag="ACG8FK_000766"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS5096397.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(25052..25756)
                     /locus_tag="ACG8FK_000767"
     CDS             complement(25052..25756)
                     /locus_tag="ACG8FK_000767"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5096398.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25779..26405)
                     /locus_tag="ACG8FK_000768"
     CDS             complement(25779..26405)
                     /locus_tag="ACG8FK_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5096399.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26731..29106
                     /locus_tag="ACG8FK_000769"
     CDS             26731..29106
                     /locus_tag="ACG8FK_000769"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS5096400.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGVYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29130..29621)
                     /locus_tag="ACG8FK_000770"
     CDS             complement(29130..29621)
                     /locus_tag="ACG8FK_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS5096401.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29808..30728
                     /locus_tag="ACG8FK_000771"
     CDS             29808..30728
                     /locus_tag="ACG8FK_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5096402.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30882..31226
                     /locus_tag="ACG8FK_000772"
     CDS             30882..31226
                     /locus_tag="ACG8FK_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS5096403.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(31416..31916)
                     /locus_tag="ACG8FK_000773"
     CDS             complement(31416..31916)
                     /locus_tag="ACG8FK_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5096404.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            32444..32527
                     /locus_tag="ACG8FK_000774"
     tRNA            32444..32527
                     /locus_tag="ACG8FK_000774"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32478..32480,aa:Leu,seq:cag)
     gene            complement(32851..>33285)
                     /locus_tag="ACG8FK_000775"
     CDS             complement(32851..>33285)
                     /locus_tag="ACG8FK_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS5096405.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11004 a         5772 c         6785 g         9724 t
ORIGIN      
        1 aatcagttgc gttttgtttt ttagaattat caataatatt attatgtaaa cttaaaaata
       61 tgacaacttc ttggtttatt accacttcct atgctacact gaagaggaat atgaaatagg
      121 agagttgaag atgcaagaat atcagtatcc acttgattta gattggacaa cagacgaaat
      181 ggtcattgta atgaagatgt gggaagatct ggaacgtgcg aatgaaaaag ggataaacaa
      241 tgaagctttt ttgaagactt atcagcagtt taaaactgtt gtcaaatcca ttggtgagga
      301 aaaacgattg ggacgggaat ttgaaaaagc ttccggttat tctttatatc gtacagtgca
      361 gcaagcaaaa aaaaatccaa acaaaacttt gagaatgaac gggtgagata aatggaacag
      421 atgattgatc atatcaagtc ctggcttgaa gaagctggtg agaaaatcaa atcttctcaa
      481 caaacattaa caatctcgca aaaatcaaat cgaacggatc tagtaacaaa tgtcgatcga
      541 aacatccaga attttctaat cgaaaaaatc cattcctttg atccagaggc gaaaatattg
      601 gcagaagaag aagggtgtaa tcaattatcg gatctcgatg gaagagtctt tatcatcgat
      661 cctattgatg gcaccttgaa ttttgtgatt gaaggggaaa atttttgtat tatgctcgct
      721 gtttatgaat ccggcgttgg aaagcttggt ttcatttatg acgtgatgag aaatgaactt
      781 ttttggggtg gaaaaggaat aggcgtgtat aaaaatgatg agccattgcc tagaccagca
      841 aataaatcct tatcggaagg cttacttggt gtgaatagtt atcttttcgg tcataataaa
      901 tatggaatcc gagaaattgc ggaaagcagc atggggacac gtatgtacgg atgcgctggt
      961 cttgaactag tggcaatctt gaaaggaaac cacattgctt atatttcaaa ccttagccct
     1021 tgggattatg cagcgggaaa tgtcttattg gaagagtttg gtatggtaac aagcggtctt
     1081 tttggtactc ctttgacatt tgaaaaaagg gaatactatt tagcagggac ttcttgtgca
     1141 tatgcagaaa tcgcagacct tttgaaaaaa gaaggctgaa aaaaaggtag aaatcattca
     1201 caaaaatgat aaaaacgagc aaaagattaa ttttctcgaa aaaatgaaaa cattttacta
     1261 tatttcatga aaaaagtctg ctataattag cagtcgagta aaatttgccc tgtcataaag
     1321 aaccaataaa aaaggagtta gttacattga attatagaaa cgatatccgt aacgtcgcaa
     1381 ttattgccca cgttgaccac ggaaaaacaa cattagttga tgaactatta aaacaatcag
     1441 atacattaga cgcacatacg caattgcaag aacgtgcgat ggactcaaat gcgctagaaa
     1501 aagaacgtgg tatcacgatt ttagccaaaa acacagctgt agactataaa ggtattcgcg
     1561 taaacatcat ggatactcca ggacacgcgg acttcggtgg cgaagtagaa cggatcatga
     1621 aaatggttga cggcgttgtg ttggtcgtag atgcgtatga aggaacaatg cctcaaacac
     1681 gttttgtatt gaaaaaagca ctggaacaac atatcacacc aatcgttgtc gtaaacaaga
     1741 ttgacaaacc ttctgctcgt cctgagcatg tcgtagatga agttcttgaa ctattcatcg
     1801 aattaggagc agatgatgat cagttagact ttcctgtgat ttatgcatct gcactaaatg
     1861 gtacgtcaag cttgtcagat gatccagcag atcaagaacc aaccatggca cctatttttg
     1921 atacaattat tgaaaaaatc cctgctcctg tcgataacag cgacgaacca ttacaattcc
     1981 aagtttcatt acttgactac aacgattatg taggtcgtat cggaatcgga cgtgtattta
     2041 gaggaacaat caaagtagga gaccaagtag cattaattaa attagacgga acagttaaga
     2101 aattccgtgt aacgaaatta tttggtttct ttggactaaa acgtctagag attcaagaag
     2161 caaaagctgg agacttgatt gctgtctcag gtatggaaga tatctttgtc ggtgaaacag
     2221 ttactcctgt ggatcatcaa gatgcgttac ctatccttca cattgacgaa ccaacattgc
     2281 aaatgacttt cttagttaat aattcaccat ttgccggtcg agaaggtaaa ttcgtaaccg
     2341 ctcgtaagat tgaagaacgt ttgatggcag aacttcaaac agatgtatcc ttacgagttg
     2401 aaccaacaaa ctctccagat gcatggacag tttctggtcg tggagaattg catttgtcta
     2461 tcttgattga aaacatgcgc cgtgagggat acgaattgca agtatctcgt ccagaagtta
     2521 ttgaaaaaga aatcgacggt gtaaaatgcg aaccgtttga acgcgtgcaa atcgatacac
     2581 cagaagaata tatgggtagt gttatcgagt ccttgagctt acgtaaaggt gaaatgcaag
     2641 atatggtcca tacaggaaac ggacaaatcc gtttgacatt cttaacacct gcccgcggtt
     2701 tgattggtta ttcaactgaa ttcctttcaa tgacacgtgg ttacggaatc atgaaccata
     2761 cttttgacca atatctgcca atgcttcctg gtcaaattgg cggacgtcac caaggggcgc
     2821 ttgtttcgat cgatacagga aaagcaacga cttattctat catgagtatc gaagaacgcg
     2881 gtacagtctt cgtagaacca ggtacagaag tatatgaagg aatgattatc ggggaaaaca
     2941 gccgtgataa tgatttgact gtcaacatca cgaaagcaaa acaaatgaca aacgtacggt
     3001 ctgctactaa agaccaaaca tctgtcatca aaaaaccaaa acaattgacg ttggaagaat
     3061 ctttggaatt cttgaatgat gatgaatatt gcgaagtaac accagaaagc attcgcttga
     3121 gaaaacaaat tttagagaaa aatgctcgtg aaaaagcatc taaaaagaaa aaataatctg
     3181 taaaaggatg aagagagaaa ccagcttgtt ataaaaaata gctggtttct ttttttgtcc
     3241 aactcttttc tgtggagaag tcatctttgt caatggacat aaattcagaa aatacgccga
     3301 aagacgttta aaagactttt aaaaaactta tttctagaga gtttttgttt cataataaga
     3361 taatgtttca cgaaaggaga tcaacttgtc cttcgaagaa aatcgaagta aaatggcaag
     3421 agaaaggagc gggaagatga acgacttatt aaaacaatac tttggctatg atgagtttag
     3481 acctggtcaa aaagaaatta tccagaaagt cattgatcag gaaaatgtat taggaatcat
     3541 gcctaccgga agtgggaaat caatctgtta ccagcttcca gctttactat tagatgggct
     3601 tactgtggtt gtgtcccctt taatctcttt gatgaaggat caggtggacg cggcgaatca
     3661 attaggtata cccgccactt ttatcaatag ctcgttagat ggttacgaaa cagctagacg
     3721 cttccaagaa atcgatagac agcagtaccg tttactttat atcgctcctg aacgttttat
     3781 catgccggat tttatccaag caatgaagcg atggaacgtc cgtatgatcg ctattgacga
     3841 ggcacattgt atctctcagt gggggcatga tttccgccca agttatttac agatggcgaa
     3901 ccagctagat caactgccaa atcgtcctgt aattgttgct ttgacagcta cagcgactgt
     3961 gcaagtagct gctgacataa aaagactttt aaaaattcca gaaaacaacc atattcagac
     4021 tggttttgaa cgagaaaatc tacgatttca agtaattaaa gatcaaaaaa aagaacaata
     4081 tctaatcgaa tatttaaaga tcaacaaaaa tcaatctggt atcatttatg cagcaacgag
     4141 aaaagaagta gaccgtctct atcatttgct tagaaaattc gatttttccg tgggtagata
     4201 tcatggtggt ctaaatgaga atgagcgaac agaaatgcaa gaagcctttt tatacgatcg
     4261 cttgcagctg attgtagcga caaatgcatt tggtatgggt ataaataaaa gcaatgtccg
     4321 ttttgttatc cattatcaaa ttcctggctc tctagaagca tactatcagg aagctggtcg
     4381 ggcgggaaga gatggtcttt ccagtgaagc catcctgctt ttctctcctc aagatatcca
     4441 agtccaaaag ttttttatcc agcagtccca aagagaagaa gggcaaaagc aaaaagaata
     4501 tgaaaaatta aaagcgatga cagaatacgt gtatattgag tcctgtcttc agcaatacat
     4561 cctaaactat tttggcgaga caagcagtcc gtgcaaccgt tgtggtaact gtttagatga
     4621 cagagaaata gtagaagtaa cgacagaagc acagatggtt ctttcttgct taaaacgtat
     4681 gggtgagaac tatggtaaac aaatgctgat gaaagtctta gcaggatcga aagaacaaaa
     4741 gctgcaagct ttaggttttg gacatttatc tacctatgga ctgatgaaaa accaatcgca
     4801 aaaagaaacg atgcagttaa ttgaatattt gatatcgaat ggctatcttc tgaccatcaa
     4861 tggtgagtat cctgttttga aagtaacaga aagagggata caagtattga aaggacaaga
     4921 atctgtttat cgaaaggaac ccaagaaagt ccagcagcta tcggatgaag aaaccgatac
     4981 actatttgaa gtgctgcggg aactgcgaac ggatctagca tcagaagcag gcgttccccc
     5041 gtatgtcgta ttttcagatt ccacattgaa agaaatgagc cgaatacgtc catcaagcag
     5101 attggagatg cttcagataa aaggagttgg ccaaagcaag cttgataaat atggcgaagc
     5161 ctttttatct cggataaaaa atgcggactc taacgttctg gataagtaga aagaacgaat
     5221 acgtaaaaat gaataaaaac aactggttaa atctgttttt tttgctcctt atgaaaatca
     5281 aatatgttat aattaaggaa ttagaatggg aggtaagcac agatggattc tttgtcgaaa
     5341 aaacttgcga tccaagtatt aaatgatgaa gcagaacgga tcaaacgatt aatccgaaat
     5401 caaaacaaca gtctctgtat ctcacaatgc aaagccttcg aagaagtggt agatacacag
     5461 atgtacggct tttcccggca agtcggtttt gctgtccgta taggagtaat cggaaagaac
     5521 gagggccagc tgatgttaag tgagctagaa cgagaattga atcagttata tagtgaggtt
     5581 taccaagaaa tttatgataa aaaagaagtt ggcaaggagg aatagacttg cctaagaaag
     5641 tgaaaaaaag gcatctatta gactatagca tcttgattcc ctatttaatt ttatgtgtat
     5701 tgggattgat catggtatat agttctacgt cgtatctatt attagaaaac ggacaaaatc
     5761 cgtccgcatc agtcattaac cagagtattt tttgggtgct tagcttgatt gctatcgcac
     5821 tactttataa aatgaaaaca gatgttttga aaaaccagcg tttgatcatg gcagccatcg
     5881 cagttttgac gattttgctg ctgattgttg tgttttttgg taaggagatc aatggggcga
     5941 aaggttggct gcaaattgca ggattctcga tccaacctgc cgaataccta aaaatcattt
     6001 ctatctggta cctgtctctg actttatcta aaagacaaaa cagtgtacaa aaagattttc
     6061 ttgggactgt gaaacgtcca cttgcaatgg ttatcggact tactgcatta gtcgctattc
     6121 ttcccgattt tggtaatgcg gcagttatct ttttgattat tttggttctg cttttagcca
     6181 gcggagtcaa ttatgtgtat actttgattg taggagtagg aggcttttgt ctaagtactt
     6241 ttacgatctg gctgatcaat atcacaaacg gaaagatctt acccggacgt ttacagtata
     6301 tctataatcg atttgctatt taccaaaatc ctttctcaga cgaattaaat aaagggcatc
     6361 aactagtcaa cggctactat gccatgttca acggaggact atttggcagg ggattaggta
     6421 acagtatcca gaaaaaagga tttttacaag aagcacaaac cgattttatt tatgcaatcg
     6481 tagtggaaga actgggagtg attatgggga ttttgatatt agcgttgcta ttctttatga
     6541 ttgtgcgcat tgtccttgta ggtatccgtt caaaagatcc gttcaattcg cttttgtgca
     6601 ttgggatcgg cgcaatgttt ttgatccaag tatttgtcaa tcttggaggg attactggcg
     6661 ttattccttt gacagggatc acgttcccat ttttaagtca aggaggctcc agtttgctga
     6721 tgctttcgat ttgtgtcggc tttgtcctca acatcagtgc ggatgaaaaa cgcaagagct
     6781 taggtttata ataaaaaaag atgtaggagt ggttcgatga aaaaagtttt agttgctaac
     6841 aggggtgaaa tcgctgtacg tgtatttcgt gcttgtacag aattaggaat taaaactgtt
     6901 ggaatctatg cagaagaaga tgaatattcc gtacaccgtt tcaaagcgga tgaagcatat
     6961 ttagtaggac aaggaaaaaa accgattgat gcgtatctag atattgaagg gatcatttct
     7021 atcgcgaaag aatgcggagc tgacgctatt catccgggtt acggtctttt gtcggaaaac
     7081 ttgaattttg cgcaacggtg cgaagaagaa ggcatcattt ttgttggacc caaactgcat
     7141 catttagata tttttggaga taaaatcaaa gcaaaagctg cagctatcga agcggggatt
     7201 gcttcgattc caggcacaga tggaccaatc gcttctatag acgatgcctt ggagtttgcg
     7261 aaacaatacg gttatccgat tatgatcaag gcagctctag gcggcggcgg acgcggcatg
     7321 cgtgtagctc acgatgaaaa gagtgcaaga gagggctacg aacgtgcgaa aagcgaagca
     7381 aaagcagcct ttggtagtga tgaagtgtat gttgaaaaat acatcgctaa tcctaagcat
     7441 atcgaagtac agattctcgg ggatacgcat ggaaacgtca tccatctttt tgaaagagat
     7501 tgttctgttc agcgccgtca ccaaaaagta gtagaagtag cgccttgtgt ctctatgaat
     7561 gaacagcaac gtcaaaaaat ctgccaggca gctgtccaat tgatgaaata tgtcgggtat
     7621 gtcaacgcag gtacagtaga atttcttgtt gaaggagatg atttttactt tatcgaagtc
     7681 aaccctcgcg tccaagtgga acatacgatc acagaaatga ttacagatat tgatatcgta
     7741 acaacgcaac tgttgattgc tcaaggtttg gatctgcata aagaaatcgg attgcctcag
     7801 caagaaggaa ttaaattaaa tggttcagcc attcaatgcc gaatcactac agaagacccg
     7861 ttgaataact ttttaccaga cacagggaaa atcgatacgt atcgttcacc tggtggtttt
     7921 ggcgtacgtc tggatgtcgg aaatgcttac gcgggctatg tagtgacacc gtattttgat
     7981 tcattgctag taaaagtatg tacgcatggt gctacctttg aaacagccat tcagaaaatg
     8041 gaacgttgct tgaaagaatt tagaatccgt ggagtgaaaa caaacattcc atttatgctc
     8101 aatgtcatca cacatcctga attccaatca ggaaatgcaa aaacaacctt cattgatagt
     8161 acagcaacat tatttgaatt tcctcgacta cgagaccgcg gaaacaaaac gatgaaatat
     8221 atcggtgaaa ttactgtgaa tggttttcca ggtatcgaaa gtggcgaaaa accattttat
     8281 gaagaaccac gtatgcctaa agatctaatc acaagaactg attacgtaac ggctaagaat
     8341 gtattagatg cgaagggtgc cgatgctttg gtcgaatgga tcaaaggaca ggaaaatttg
     8401 ttgttgacgg atacgacatt tagagacgcc catcaaagtc tgctagcaac tcgtgttcgg
     8461 acaaaggatt tcaagcagat tgcacgtttg actggagaag gtcttcctga actcttttca
     8521 agtgagatgt gggggggagc aacatttgat gtggcttacc gctttttgaa tgaagatcct
     8581 tggcaacggt tgagaaaaat tcgttcttta atgcctaata cgttgctgca aatgcttttc
     8641 agaggttcaa atgccgttgg gtattctaat tatcctgaca atgtattggt ggaatttgtc
     8701 aaagaagcag cagcgcaagg aatcgatgta tttcgaatct ttgatagttt gaactggact
     8761 ccacaaatgg aaaaaagtat ccaagctgta cgggatactg gtaaaatcgc agaggcagct
     8821 atttgctata ctggcgatat caacgatcca agtcgggcaa aatacaatgt tcagtattat
     8881 aaagacatgg caaaggaact agaacaactg ggtgcgcata tcattgcaat caaagatatg
     8941 gctgggctgc ttaaaccgca ggcagcttat cgtttgatta gtgaattgaa agaaacgaca
     9001 gatctgccaa tccatctgca tactcatgat accagcggga atggaatcat cacttattct
     9061 gctgcttcaa aagcaggagt agacatcgtt gacgtggcaa tgagtgcaat gagtgggaat
     9121 actagtcagc ctagcatgag cagcttgtat tatgcactgg tcaatggtcc acggctgcca
     9181 gaaatcacga ttgaaaatgc acagaaatta aaccattatt gggaagatgt gcgcatgtat
     9241 tacaaaccgt ttgaaaacgg cttgaatgct cctgaaacag aagtatatat gcatgaaatg
     9301 ccaggaggac agtattcgaa tctacagcaa caggcaaaag ctgtcggatt aggtcatcgc
     9361 tgggatgaaa tcaaacaaat gtatcatact gtcaacttga tgtttggcga tatcgttaaa
     9421 gtaactccat catctaaagt agttggagat atggcactat ttatggtcca aaatgatctt
     9481 acagaagaag atatttatga aaaaggcgaa acactaagtt tccctgaatc tgttgtgact
     9541 ttcttccaag gagaactagg gcaacccgtt ggcggatttc ctaaaaaatt gcaaaagatt
     9601 attcttaaag gcagacctgc attgaatgaa cgtccaggtc ttttggctga atcagtcgac
     9661 ttcaacgaag taaaaaaaga attggcagaa aaaattggct atgaaccaaa acaagacgaa
     9721 gtattaagct atttgatgta tccgcaagtc tttttggact atcaaaaagc atataatcaa
     9781 tttggcgatg ttacattact ggacacacca acatttttcc aaggtatacg tttgggtgaa
     9841 acaatcaatg tacaaatcga acgcggaaaa acgcttatca ttcgtttaga tgaaatcgga
     9901 gaacccgata tcgaagggaa tcgcgtactg ttcttcaatt tgaatggaca aagacgtgaa
     9961 atcactatca aagataattc gatcatcagt acggtccaaa cccgattgaa agctgaaccg
    10021 acaaaccgag aacaaattgg agcaacgatg tctggttctg ttttggatgt attagtgaaa
    10081 aaaggcgata acgttaaaaa aggtgatacg ttgatgatta ctgaagcgat gaaaatggaa
    10141 acggccatcg aagcacgttt tgacggagaa gtcgcacatg tatatgtgtc ttcaggagac
    10201 actatttcgt ctggtgacct tttgattgaa gtgactgaga aataataagc gaaaggtggg
    10261 gaaacatgaa aagactgggt ttgttttggc tgattctgct attagttatt gtggcattct
    10321 acatggaacc agtcttgttt ccgccaaaac ttgaagtgaa ggaaaactct gatcaacaaa
    10381 tatcaaacca aatgacaacg tggaaatacc aggagcttaa agcaactggt ttttctactt
    10441 atatcggaaa accgatcgat cagctggaaa aggattttgg ccaagcctat gatcgattaa
    10501 ctagcggatt tggttttgaa acaagatatt atgaaaataa agctagtcat ctcacatttg
    10561 aagcgaacat ccaagacagc agagtcacaa ctgttaaagt actgcaaaca gggcctgctg
    10621 atatcgcgcc gttttattta gggatgacga tgcaagatct tacgaaaatc actactatct
    10681 atactaattt cacttttgag taccatgata cagaagttgg aatcgaacta atggaagagg
    10741 atatgaatta tcgtccgcta attgcttttg ataacgacac ttttgctatt ttattttttg
    10801 atcaaacaac aaacggccta ttttcagttg tgtatttaga taaagatagt cttttgaaac
    10861 tattgccata tcaagtgttt ggtgaaggcc ttccacatta tcaagcagat aaatcggctg
    10921 attgggaatc aatcaatcgc gttaaagaac gaaaaagtac gactttatta aatatgctta
    10981 gaagagaaga cgaacttccc atatacaatc aaacacttgc ctttcaaaac cacagtcagc
    11041 tgctactaca tgattttttg acaaggcctg aggaaatatt gtcagaggaa cgtatggctg
    11101 attggcaaaa atcgctaggc agtgctcaaa cgacagtgaa attctcactg acgaatgatg
    11161 aattggagaa gttaatcagt aaacaaaaat tagaaaatac tagtgggcta tttattcatc
    11221 cggcgattga cgctactttt tcgtttttat attggtacag cgatccttac aaccatgacc
    11281 gtttcatgga cccgggaacg gattctgtag gcattgcttt ttctaaagaa aatatgttag
    11341 tcttattgca agaagaaaac ggagaaagta gtgatagcag tgatcgttaa cgaagagctg
    11401 tttcaactgg aggatcaatg cctccaagtc gctgaaatga taaaaaagag taaaacgatg
    11461 gcttcttatc ttgaacataa acagtcaatg gaagaatctg caaaagtttg ccagttgaaa
    11521 gaagcgttct tgtctaagaa agaagcattt gaaaatgtag cggcatatgg tgagtatgca
    11581 cctgattatc gtgaaaaaaa acgagctgtt cgaaaagcaa aaagacagct tgatatggat
    11641 gagcatgttg cagctttccg atatagtgaa acacagctgc aaagtctgtt ggatcaaatt
    11701 gtccaagtac ttgccgaaga agtttctaat gaaatcaaag tagatgcagg tagcccgttt
    11761 tttgaaacga aaaaacactt tggatgcgga ggtaattgtc atgcaattag acaatagtga
    11821 atttgaaata acaaaacgac gcgggctgat cgtatgggtc tacagcttaa aacaattaaa
    11881 gaatctgaaa aagttcggct tgttgcatta tgtctcaaga agaatgaaat atgtcgtgct
    11941 ttacttaaat gaagagacat tggaagaaac agaagaacga atccgtaaat tacattttgt
    12001 tcgaaacgta gaacgttctt accgaccgga tatcgaaatg aattttgccg aaaaaatagg
    12061 aaacaaaaca gtattaaaag acgaaggttt cgaaattgaa gagctaaata cagaaatccg
    12121 attagctgaa catctttaaa aatctcttat gagcagtttt taaataaaaa ataacttgga
    12181 taacgatcga tttttgatca ttgtccaagt atttttttgg tatactagta ccagtaccat
    12241 ttctaaggag tgagtagatg cggataatat caggaaatta tggcgggcgc cgtttaaaaa
    12301 gtttagcagg ggcgaacacc agaccaacta cagataaagt aaaagaatcc atattcaata
    12361 tgattggacc atattttgat ggagagacag tattggatct gtttgcagga agcggtggtt
    12421 tagctatcga agcagtatct agaggctgca gtcatgcggt atgcgtagac aaaaattacc
    12481 aagcgctaaa aatcatcaaa gaaaacatcg aaattacgaa agaaccagag aaatttacca
    12541 caataaaact tgatgcggac aaagcaatcg aacggctagc agcggagaaa caacaattcg
    12601 actacttgtt tcttgatccg ccatatgcaa aacaaaaaat aatcgatcaa atccataaaa
    12661 tggaagaact gggacttttt tctaaaaccg cagttatcgt atgcgaaacg gataaaacag
    12721 tcgaattgcc agaaactgtg gctggattcc atcaaataag aaaacagatt tatggcataa
    12781 cggccgtaac tatctataga aaagagggaa atcaatgaaa cgtgcattat tcccaggaag
    12841 ttttgatcct tttacaaaag gtcacttaga tacggtggaa cgtgcggcta aattattcga
    12901 tgaggtagta attggtgttt ttatcaatac ttcaaagaaa agtttgtttc ccccagaaga
    12961 aagaatgaca ttgatcacaa aagctgtttc tcatcttcct aatgtaaaag tcatgcatca
    13021 agaaaatcaa ttgacggtag aaacagcaaa agagattggt gcagatgcgt tgatcagagg
    13081 catacgaagt atcaaagact ttgaatatga acgagagatt gctcagatga atcatcatct
    13141 atatgatgaa ttggaaactg tcttcttatt agcaaaacca gaatatagtc acgtgagttc
    13201 cagtatttta aaagaagtac tgcactttgg cggagatgtg agcagctact taccgccagt
    13261 gatcaacgaa gctttagctg gaaagcgtga atcaaatgaa agctaaacaa tggttgaaaa
    13321 cagtactgat tatcatattt ggaatgttga tcattggatt tgtagtagtt cccttgccgt
    13381 attatgtaga atcaccggga gccacgatcg atctgaaaga actaattacg gtcaatgata
    13441 aggaagataa aggttctggg tctttctctc ttacctctgt tggtatcagg caagctacag
    13501 gtttcttagc tttaaaagct aaaatgaccc cttttgaaga attgatcagt gaagaagaat
    13561 tgactggtgg ggcgacaaat gaagaatata atcaaatgca acagtattat atggattctt
    13621 cccaaaatgc agcaatcgaa caagcgttaa aacttgcaga caagccttat aaaatggaat
    13681 tcaaaggcgt atatgtcatg agtgtagaga aaacatcgaa tttctttggc aagctttcag
    13741 ttggagatac agtaacaaaa gtagacggga aaagctttca aagtacagaa gcatttatgg
    13801 attatgtaaa aagccaaaaa gttggacaaa caatcgaaat cagttatttg cataatggtg
    13861 aagaaaaaac agcttctggt gatctgatcg agttaccaac agataaaaag gcaggaatag
    13921 gtatcacgct tactgaccat accgagatcg aatcagatac agatgttcgt attaattctg
    13981 gctctatcgg tgggccatca gcgggattga tgttcacttt agaaatctat gaacaagtca
    14041 ctggtaaaaa cttgcgacat ggtaagcaaa ttgcaggaac tggaacgatc aacagtgaag
    14101 gagaagttgg ccgaatcgga ggaatcgata aaaaagtggc aagtgcggat aaagctggcg
    14161 tagagatttt ctttgcacca gatgatacga tttcagacga tgttaagaaa gaatatcctg
    14221 aaataaaaac caactacgaa gaagcaaaag ctgccgctaa aaaattgaat tcctcaatga
    14281 aaatcgtacc agtaaaaaca gtacaagaag cgcttgacta tctagagaaa atgaaatgaa
    14341 actaaataat taaagagggg ttgtgacacg agttctgtca caacccctct ttttcgtcaa
    14401 aaatcttcac gataaaaata aaaacaaaag aaaaaattga cgtaattaga tgttgaaggg
    14461 acgattttgc cagagctcac tgtttcttca attttaaatg aaaggagtaa tgaatgattt
    14521 ttactggtca ttctttgaaa aaagaacaat gatagaaaaa atcagcaaga aaatactagc
    14581 ttcacccaaa ttgatcattt ggatgtctat gatagttctt atcttttgta tcgcaagtgg
    14641 ttttttatta tcaaatttgc tattttatag agaaacagaa gagactgctg caaaagaaat
    14701 actttcttcg tttagttcca gtggagaaga tccatcagct gaaggaaacg atgtagaaga
    14761 ggatgctcgt ccaaaggtca tgtacaccga tatcaaagga agtgtgaagg agcctggtat
    14821 ctattccttt tcttcagaag aacgggtcta tgatgtacta aagcgtgcag gtggattatt
    14881 agaagaagcg gatagcgatc gaatcaactt ttctgcgaag atagaagatc agcaagtcct
    14941 atatatccct gctgtaggtg aagagctgcc agaacatctt gatcagttgg catcctcgga
    15001 aggaaaacag cctacagcag attccgagcc tcccaaaata aatatcaata cagccactcc
    15061 atcagaactt cagcagattc ctgggatcgg atcagtcaaa gcgcaagaaa tcattcgatt
    15121 tagagaagaa aacggatcct tccagaaagt ggaagacctg caggagattt ctggtatcgg
    15181 tgaaaagaca gttgaaaaac taaaaaactt cgttacaatt aaatagaatg attcaaaatc
    15241 gactggcacg agcaataaat ggacgctaat ggatactaag aataatccaa ttctaagagc
    15301 ttggcaggac gaggaggtag tatttttgga taggaaatag cttgattgtt cgcaaagaaa
    15361 cattaaaaaa accaatgctg aatgtctatc tccaaaataa aatatctggg attcatatta
    15421 tgaatacggc tgtcagcggc aataacagtc aagctctacg agaacgcttt gccaaagatg
    15481 tacttagcta tacagcagat aaagtattta ttctcattgg gacgaatgat ctagcagagc
    15541 ataaacaact ttctaaagaa acgtaccaaa aaatctgcag tggatgatag ttcagctgaa
    15601 aaaagactat agttctgatc atgctttctt tatcacgcca ccagcagtcg atgagacaaa
    15661 acaaaaatat cggaataaca agttgatcaa agaatatatc tctgttctca cagaagtagc
    15721 aaaacgccat gattgtgaag tatcgatttt tatacggaat ttcttgaaca aatggattcg
    15781 aagaattttt ataatgggct aatggatgac ggacttcatt tcggcagtga agggtaggat
    15841 cttttgtcag acaggatgat tcgttatatt taaagaatat tttgtttaag caatgagtac
    15901 tttttaatca acttgaaaaa acggaggaat ataaaacatg ttagaaaaat cttcaaaagc
    15961 atgcgatacg aataaagaaa aagttactga taagtcacat aatgaacgca ttccttggga
    16021 ccagtacttt atggctcagg cagtactgtt atctttacgt agtacgtgta cacgtttaga
    16081 agtaggagcg acactcgttc gagaaaaaag gatcattgct ggcggctata atggagcagt
    16141 cagtggagat gtccactgca tagatgaagg gtgttacgtc gtggatggac actgcttgcg
    16201 aacgatccat gcagagatga atgcactcct acagtgtgca aaacttggta tcccgacgga
    16261 aggttcagaa atctatgtca cacattttcc atgtttggcc tgtacaaagg ctttgttgca
    16321 agctggtgtg aaagggatca attatctcca tgactatcga aatgatcctt atgcccaagc
    16381 gttgatcgaa caattaggtg tatctgtaca ccaagttgct ttagactcac agcatttttc
    16441 aaaacttcaa aatgaattaa caggaaaaca tgtctagcgt tttagaaagg catgttcgaa
    16501 atcggttgat ttttccggca attacgtcga tgattatctg tttgttgtat tacactgaca
    16561 attggggtta tcgtttgctt cttgccggaa tttttttatg gttttcgcta aaagaaggac
    16621 gttttatcgc gtacttgtgt attttgtgta gcataacggt atccatttct tataggctac
    16681 aagagtattc atttttagag gaaaaaacaa catctgcgca aattactgta tttacagata
    16741 cgattcgtgc aaatggtaat tttttgacaa tggaaggaag gttatcagag aataaacaaa
    16801 agctccagct ttcttatcaa gtaaacacag aagcagaatt gaaaacatgg cttaacttcc
    16861 gaggaaaggg aatagaattg actgctgaag gcgagttcat ctctccagca aaaaagcgga
    16921 atctttataa ttttgaccaa gaagcttact tcaggaacca tagaatttct ggacggttcc
    16981 aaattcaaaa gattgaacag atcaccatgc aaaatcactg gcgaaactgg cttcagagaa
    17041 aaagaggatc ttttatctcc tttgtccaat ctgaatttcc gcaaaaaaca agtgtatata
    17101 ttaattcttt attatggggc tataaagatg caggattcag agaaccagaa gaaattttta
    17161 gggagagtgg tttgctgcat ctgttcagtc tatcgggtct acacatccaa ttttatttag
    17221 ggtggctgta ttatttattt cgacgtatcg gatggccact acacgtcagt attattccgt
    17281 tgagtctact aactatctgt tatgtttcta ttgcaggaag ttctatttct gttttgcgtg
    17341 ctagtttgct gtttttattg cgattgctag tcaaattgct gaataagcat tattccacca
    17401 tggacttatt tgcagttgtc ttttggcttc ttttactatt tgatcctcga tttctttttc
    17461 atgcgggtgg acagcttagt tttttcatga gttttttgtt tatcctgatt tcttttgatg
    17521 gagattggtt gaaaaaaacg gcctctcacg tcctgtttac tatgatcacg atgccgttga
    17581 ttgtatggca tttttatgag tggcctattt taggtagtct ttttacatta ctgattgcac
    17641 cggtattcaa gtttcttttt cttcctggtg tgttatttct cacactcttc aatcgctggc
    17701 tgccggaaac tttacttttg ctgatagaag agggattaat cttatttgag cgaataatcg
    17761 atttttcttc agggtcgtct ctcattatag gacgtttgcc ccttgtgcta tctgggatgt
    17821 atattttcgg attactgatt tgtatggata ggttgaaaga acaacctgtg aaacacttgt
    17881 tttatgttgg tcttttttct tttatttttt tgactttgcc atttttacgc ttttcttcaa
    17941 cgatcgcttt tatcgatgtc ggtcaaggag acagtatctt tttgcaaagt ccttttcgaa
    18001 aagaaacgat tttgatcgat actggaggtc agttacactt tgaaagagaa aagtgggcgc
    18061 aaggtatgat acgtcctcct gctgaaagaa atatcgttcc atatctaaaa ggtcagggga
    18121 taagtgtaat cgataaactc atcttgacac acgacgatac ggatcatgtg ggagaattac
    18181 ccacgttatc tcagcatttt actatcaaaa aaatttatat cggttggggc gctggacgaa
    18241 atgagccatt acaaaggcat ttatctgaag ctcaaaaaaa tggtactgag atcattgaaa
    18301 tcaaacatgg ggacaggatc agcggttatt atgatcttta tgtattgact ccttttgaaa
    18361 agggagaggg aagaaatgaa gattcgattg gtctatggat ggaatataac aataggcgct
    18421 ttttattttt aggagatttg aatcaagcaa tggaaaaaca attgcttttg atttatccta
    18481 atctgaaagc cgatgtagtc aaattaggtc atcacggtag ccgtacgtca agtaacacag
    18541 attttctttc ccatattgaa gcaaaacatg ggattatatc ctgcggcgta aataatcgtt
    18601 atggtcaccc gcacagcgaa gtattggaga cactagaaga aaatcagata aaaacatggc
    18661 gaacggatca acaaggaatg atcacctatc gctggcatcc agtttttcat cctcatgggc
    18721 tagtggaaac aatgatagac tagtaattct catctgctca tggtaggata atttttgaaa
    18781 gtggtgagta atttgagtat acaagcagag ttacaaaaaa tacgtaaaga aaaacttgca
    18841 tcttgttact tgattttagg aacagaaaag tttctccaag atcaagttcg gacagaaatt
    18901 gtcaaaaaaa tccaattaga gggcgaagat gacttaaatt ttttaagttt tgacatggaa
    18961 aatgcaagtt tagatgaggt agtagctgaa gcagagacgt tgccgttttt cggagatcaa
    19021 cggttagtat tcgtagagaa tccttatttt ttaacaggtg aaaaagtaaa caatggaatc
    19081 gaacaaaata ctgatttatt gacggcttat ttgaaagaac cattagaatc gactgtatta
    19141 gtattctttg cgccatatga aaagttggat gaacggaaaa aagtaacgaa acaattgaaa
    19201 aaaacagcaa ttatcatcaa tgtccaacag ttgaatgaaa aagaagtacg tcaatatctg
    19261 atgaatacct tagagaattc ctcaatcaaa atggaacgaa gtgcaattga tttattcttg
    19321 aggttgaccg acttagattt atccaaaatg atgagagagt tacagaaatt ggtcctttat
    19381 ggacaaaatc aacagcaaat cacggtaaga gaagtagaag aattagttcc taaaacgtta
    19441 gaacataata tttttgacat gacccaatat attttaaaag ggaagacgga acaagctttg
    19501 cgtttatacg aagatttagt tatgcaaggg gaagaaacaa tcaaaattaa tgcgattttg
    19561 ctttctcaat tacgtttatt tttacagaca aaatttcttg taaagattgg ttatcagcag
    19621 gcaaatattg ctgaaacatt gaaaatccat ccatatcgtg taaaacttgc catgcaggaa
    19681 gtaaaaaaat tcgatgaaca tttgctggta cagttgtttg atcaattagt tgagatggat
    19741 tatcaaatca aaacgggaca aatcgaaaaa gaattaagtt ttcaactatt cgttttgcgc
    19801 acagggcagt tgttaagaaa tcgataagaa gaaagacgag ggcaaagcat gcgggaaggt
    19861 cgtttgtttt tgtccttttt ttgacaaata aaaaaatcgg ccgaagccga ttcttttatt
    19921 tagcgatttt tttgcttaaa cgagatttgt cgcggttagc tttgtttttg tggattagac
    19981 cttttgattc agccatatca atagctttaa cagcttcttt atataacgca tctacgttgt
    20041 cagcacctga agctacagca tcttcaaatt ttttgattgc agtacgcata gcgcttgttt
    20101 gagatgaatt tttcacatta gcgttttcgc ttgtacgaac acgtttgatt gcagattcaa
    20161 tgtttggcat tagtttcacc tccgataact taagttctta cgcgtgttac cgcataattc
    20221 aacattcatt attataccta aaggcagcgt gctttgcaat atgaacatga caagtttttt
    20281 tccttaacag tcttttatta ggattttttt tcagtaaaaa cggatagtac aagctgaaaa
    20341 aaatcaaaaa gacgctattg tgggaggcaa tagcgtcaaa ccaaactaga tttttattta
    20401 tctatttaaa caaagtttga atgggaatgt ttggtactaa gttattatat aacaaagaga
    20461 accgtttgca aagttttttt tgaaaaggga tacgattccc tgttgttctt tgtttttcta
    20521 ttaagaaact gcagttattt tataaccagt atcctttgat cagtaaaggc tattctggtg
    20581 taccgattat tttaatgtat tcatcgatat tttttatcaa catttttttt atgaccaact
    20641 ggttccgacg gttccatttg ccacttactt ccagcacata tttatttagg ggcaaacgta
    20701 agatatattt tgccaaatag cgatctttaa gcaggccatt gatcacacgg tcctcaagct
    20761 gcaagttgat cccagtcagc atcattggct gttcttgtaa aacgtacgta tcaattaaaa
    20821 gaccttcaaa agtttccaca agcgtcttcc tttcttttca aagtaacctg aaaacaggca
    20881 ctaattcttc cattttgata ttcgttgaag tggatgcgat cattctttct tttgtccact
    20941 gtaatctagt ataacaaaaa agagcagaag agccaaaaag aaaaaagagc atcgaattct
    21001 ataggaagtc tcattggcac ggcctaaatt attacaaaac attgattaaa aaaagcccct
    21061 gcatcgcagg agcatcccct atatcgtttt aataggtcaa tcaaaaagca gtatcaaaca
    21121 cattttgtat caaatcagga aataatagat aagctaagcc cagaatcaaa agtaccgcta
    21181 gaagaaacca gccgattgtt cctatgaaat actttaattt ttcgaacatt gtatcacctc
    21241 gttgtcagta tatcacggat cagtaaattg ggggtgttaa gaatctccta aaatagacaa
    21301 aatttgttct attttcctaa agcaaatact tgtaagatac agtaagtgat accagaagca
    21361 atgaccggtc cggcggcgat tcctcttaag aagacaacac ctaaaatcgt gccgaaaaca
    21421 agtgcaacag tgatttgtgg atcagaagca atcaatccta cacccttgga agataagaca
    21481 gccactagcc caccgcaaaa aacagctacc cagccaatag gggatttaaa ggcttctaat
    21541 agatcacgta gaccgatttc tcctgttgcg atagggatta gaatagccgc tgtgatgatc
    21601 gtcaccccaa tattgattcc tttgccttga agccaaggga aaaaggaacg ggaggctggt
    21661 atgagcttca taatcaaaac aaaagctgcc gctatcatca gactttgatt tttagctaaa
    21721 attgctacaa gtaaaataat tcctaaaaaa agccaacttt ccatgaaata tattcctcac
    21781 tttcttcgaa acaatcataa catgttttta gaaagttaca aaagtaggaa ttcatccttc
    21841 aagacctatt cataagctgt ttttttcata aaataaacaa aatcataaaa aaacactttg
    21901 cttatttgac agaagacagc cgcgctttgt atagtggaca agacttacat ttttttgaag
    21961 aatcagaaag atcaggtaaa ggtcttagta aacgcaagcc attcagaata accaatattg
    22021 tgcttccttc gtgtcctaca actccaagag gtagatttaa aaattgcagg aagttagaag
    22081 caatcaacaa gagaataaca agaatcgaaa aaataatatt ttgtttgatg atccgccgca
    22141 tttttaaaga tagccgatga ctgtagacaa atttatttaa gtcgcttttc attaagacga
    22201 tgtctgcgat atccatagcg gcatctgtcc cttgccccat agcgacacct aaagaagcgg
    22261 ctgctaaagc aggcgcatca ttgactccat caccaaccat cgcatttact tggtaaagtt
    22321 cattctcttt tttgactagt tgtgtttttt cttccggagt acagcttgca tagtaatgtt
    22381 caatccctac ttgttcagca atggcctttg ctgtgccaga atggtcgccg gttatcatac
    22441 ttgtatggat gttttgggat ttaaagtagg aaattacttg ttgggtattt gcttttggaa
    22501 tatctagcag acctaaaaca gcaattaact gctgatcttt agaaagataa atcacggttt
    22561 tgccttgatt ttccaaacgt tcgatcattt ccgcaatctc tttgctgatt atcatcgtct
    22621 caggatcata tgcgtgcttg ccaactttcc attgcgcacc taaataggta gtttgtaatc
    22681 caaaaccagt aatttcttct attggtaagt attggatttc ttcaggtatt gtgcaatcga
    22741 aatattgcaa aatcgcatga gcaagtggat gcgtcgtttt ttgttccata gcaacaagaa
    22801 gttgttgtgc caaatccttt tcatcaaaga aaaagctgtc agttacaact ggttttcctt
    22861 tagtgagcgt tcctgtttta tcaaaggcaa tcgcttttaa ttcagaaagc ttttctaaat
    22921 ggataccgcc tttgattaaa atcccatttt ttgcagaatt tgaaagagca gctagagaag
    22981 ctggtgtagc agatgcgaca agcgcacaag gagaagccac aactaataat accattcctc
    23041 gataaaagct ttcatcccaa ctccaaccta ggaaaaagtg ggggagaagg atcattagag
    23101 gaacagcaag caaaatcaat tttacataat tattttcgat tcgttcgata aatgaagctg
    23161 tttgagttgg cgtattctgc gcttcttcta ctaattggat aatcttagca aagacagttt
    23221 cggaactcgt ttttgttact cgaatcgtga tgggatttcc ttgattcagc gttcctgcat
    23281 acatctcatc gcctaaatat ttttctacag gcacagattc accgcttaat gcagactcat
    23341 cgatcgttgc ctgtgtgctt tctaaaaagc catctatcgg aacagctgct cctttagcga
    23401 caaataccaa gttatccacc tttagttgag aaacatctac ttcttgcatt tgtccatttt
    23461 cttttaataa ttgggctttt tgtggttgaa gattcatcaa agcagtaatt tctttttgac
    23521 ttttattagt tgtgtattcc tctaatgcac cgcttaaaca aaagatgaat gtcaacattg
    23581 ctccttcaaa gtaattaccg atcaaacagg caccaatagc agctaaagcc atcaataaat
    23641 ccacattcag tgtccgatca ttccacaatt ctttcaagcc ttccgatgtt tgtttatatc
    23701 caccgatgat aatggcactg atataaaaaa gtggggtgat ggtaagagag aatcggtcga
    23761 agataaaacc gaagatcata aataaaaggc agccgattgt tgctgcaaaa gcttttttct
    23821 gattagtcat cgttattaga cctccttgtc aaaacaagga tagcataata aaaatgtaat
    23881 tgataataat aacagctaaa tgagaacgat aatggttatc atttggtgat taagcttgat
    23941 gttgttttta ttatttataa tgattattat taaagaaaat aaaaaaccag aacatttctc
    24001 aaataagaga agtgttctgg ttttttctac attgcaataa accaataacc aatcaaaatg
    24061 ataccaagga taatacgata ccagccaaaa gcggtaaagt cgtttttctt caaatagctt
    24121 agtaagaatt tgatagctaa gacggaaacc acaaaagata cgaatgttcc tacaagaaga
    24181 acaacagttt cttccatttt gaatgaattc ccttcagcta aatatttgac gatcttcaag
    24241 ccgcttgcgc caaacatgac aggaatccct aagaaaaatg aaaactctgt agcgacaaaa
    24301 cgcgaagcac caatcaagat agatcctagg atcgttgcgc cagaacggga tgttccggga
    24361 acaagtgcca agacttggaa taacccaaca agaagagctg cttggtacgt aaagtcattc
    24421 agagaagtcc aacgaggtgt tttgtttttg ttgcgttttt cgacaataat aaaggcaata
    24481 ccgtaaacga tcaaaacaat ggcaactggt aagaacttgt ggaaatttgc ttctaaccaa
    24541 tcatccaaag gcaaaccaat gatgatggct ggtatgcaag caacgatcac tttaaaccaa
    24601 agtgtccacg tatctttttt ttctaccgtt gatttacttg gagaaaatgg gtttaacttg
    24661 tgaaaataaa gaacgacgac agccaagatc gcccctaatt ggataacgac attgaacatc
    24721 gacataaatt gtgaactggc atccagtttg atgaattcat ctgctaggat caaatgacca
    24781 gtactactga tgggcaccca ttccgttatt ccttcaataa taccgagaat cactgctttg
    24841 ataatatttg caaaaaacat aaattccttc cttcccaatt aagatacatc ttatagtata
    24901 ccgttatttt ttgagaaacc caatctgttt tttaatatta agaaaaactt gagaaataaa
    24961 gcagataagt acacgaagag aaaaaagaag acaaagccct cgttcagact ttgcctttta
    25021 tgattctctg gtaaaaatta aaatatcccc attatagaaa cgagtcgcga ctatgtgttt
    25081 tcccgttatc gatataagac gtatcattga tgccgtggat agaaaatcga ccgtttgcat
    25141 aagtgacctt tgttacgctg ccatttaaaa gcccagcacg aacaggctgt ttaggatcga
    25201 tcagattaag caaaaaggcg atcgtcaaac catgagatac gactaaaaca tttccaccac
    25261 catttttttc gcagtgatgg gcgatatctt caaatcctgt ccatacacgg tctcgcaatg
    25321 tttcataagt ttccgcccac ccagcggtat ctaagttgta tacagcatta gcaatttgtt
    25381 caaaagaaac ttctgtggta aacatttcat catatgtttt aaaattcaat acacgaggga
    25441 tcacacccca catttccatg tcatatccac cttcaagaga cccgaaacac cattctctaa
    25501 tccttttttc aataaaataa ggaatttctt ttcccttaga atgttctcct agaataatac
    25561 gggcagtttc gattgcacga ccactgtcac tagaataagc aaatgaaaag tcgacatttt
    25621 ttaacccgcg acctaaataa tgaatgcctt cttgtccttg ctttgtgagg ggtgtatcac
    25681 accagccttg tactcgttca attgtattga acatcgtttt tccgtgtcgt acgatataaa
    25741 gtgtagtttc tgtcatttta ttctcctcct aagtcgtttt aacgattgaa aaaaggattt
    25801 gtcctttttt cctgttcaat cgtcgttgca tcgccatgtc ctggatagac tgcaaattca
    25861 tcaggtaata caaacagctg ggtccgaata ctatgaagta attgttctaa attccctgta
    25921 tacagatcgg ttctaccgat acttcctcta aacagggcgt cgccagaaac gacaaaatca
    25981 tcaaaaataa agctgacgct tcctatagaa tgccctgggg tagggacgac gcgaaaagac
    26041 atgtttccta aagtataagt tttcatttca aattcatatt ctgcaggcga gacaataaca
    26101 tcagcaatat catcatgtct accgagtcca gaaagattca aaatcggatc acctagccat
    26161 ttttgctcta gtgggctgac ataaactggg atttgataat acgcgcgtaa aggttccact
    26221 gcaccgatat gatcgtagtg agtatgggta agcaaaatcg ctaccggctg ttggtttgtt
    26281 tttttgattt cttgtttgat tcgttccgca tcttctccag ggtcaatgat caaaagtgct
    26341 tcttcgttgt agaccaaata acagttttct tcaatcattc ctgtttttaa tctttcgata
    26401 tgcatgtgca ttcctccttg atagccaaaa cttctttcat ccattagtat aacgcattca
    26461 ttgctaggaa acaaaaacag gtacttattt ctctttactt atctgaatta tcagactata
    26521 tgaaaagtat ttggatattc taaagagaag gaaagtaagt tttcatagca ttttaatatg
    26581 tccttgttac attagattca tccaaaaatc caaattctat atcctatatc cctgcctaat
    26641 cagcagggtg tttttttatg tttggaaaga gagctctttt tgctaaacta ggttttttca
    26701 ggtaatctta gagagaaaga ggtggtggaa atgatcaaac agtcaaaaat atctgtgcgg
    26761 cacttagtag aatttatttt gcgacgtgga agtatcgaca atcgtaaaaa aagtaaccat
    26821 actgcattag aaggagcgag aatccaccgc aagctccaaa aagatgcggg agaaaactat
    26881 gaaaaagaag tctttttaaa aacaacagtt caattagatg aatattacct gatcgttgaa
    26941 ggccgtgcag acggtatttt caaaagagac ggtgtttatt atatagatga aatcaaaacc
    27001 tccgaaccac gatttgaaga tttagaacca gaacaagtgg aacttttctt tcatcaagca
    27061 agggtgtatg cctatatcta tagtcaagaa catgatttac aagaaataaa tctgcaattg
    27121 acttacttcc agacggcaga agaagtgatc acgagaaagg ttgaacatca gacaagagag
    27181 cagttagaaa ctttttttaa aaagctgact gaagattata aagaatggct tgtttttcaa
    27241 gaaaactggc gcactgtacg caatgcctct ttgatggctt tgaaattccc ccatgatgag
    27301 tataggaaag gtcagcgtga actagcggta gctgcttata aaacgatccg tacgaaacag
    27361 aaattgtttg tagaagcacc taccggaaca ggaaaaacaa tctctacgtt attccccgct
    27421 ttgaaagctg taggggaaga agagggagaa aagatctttt atctaacggc taaaacaata
    27481 actagacaag tagctgaaga tgctatgaca gcattaaaag atacaggagc tgaagtaaaa
    27541 agtgtcactt tgacggcgaa agacaagatc tgctttttag atgagacgac ctgtaatcca
    27601 gatcagtgcc catatgccaa tggttattac aatcggatta atgaaggttt atgggatctt
    27661 ttgaatcatg agaatcagat cactcgtgaa gtcatcgaaa cttatgcaag aaagcataca
    27721 ctttgtcctt tcgaactatc attagatgtc agtatctggt gtgacgtaat cattggggat
    27781 tacaattatt tatttgatcc aactgtttat ttaagacgtt tttttgaaga tgaaaaaaac
    27841 gaggactatc tgtttttgat tgacgaagca cataatttgg tgaatcgttc ccgagagatg
    27901 tattcggcag aactatctta tgaaaaaacg aaacgaagta aggaagctat accaaaggaa
    27961 ttcaaaaaac ttcatcgccg tttcaacaaa ctattaaaag aatttgattc gatccgagaa
    28021 atagcaaaag aagatcattg ggattatcat catcaaaaag caccggctga atctttagta
    28081 aaggcaggtt atcaattaag cgaaaaaatt aaagaatggc tggcagaatt tcctgagcac
    28141 ccatcacaag aacaattgct tccttattat tttgatttgc ttcatttttt gaaggtcagt
    28201 gaattttatg atgatcacta cgaaacaaca gtcgaaaaaa cgtatcgcga tctgatagta
    28261 aaagagtttt gtattgatcc aagcttgttt cttgagcagt cgttggataa aggaaaaagc
    28321 agtattcttt tttctgctag tttttcacca ctctcttatt atcaggaaac actgggggga
    28381 caaaaaagtc tggcttacaa actgccgagt cctttcccag aaggaaacca gcaagttttc
    28441 atcgcaaatt acctagagac gacttatcga aaaagggaac acagtctggc aaaattagta
    28501 gagacgattc atgcttttgc tgatggaaag gtcgggaatt atttcgtttt ctttccttct
    28561 tatcaatatt tagatcttgc ggtggaagca ttcagacagc aatatcccga cagcaatgtg
    28621 ctaatccaag aaacggacat gaatgaggaa gaacgcgaac gatttttaac aaaatttcaa
    28681 acaaatcccg ctgaaacatt gattggattt tgcgtacttg gcgggatttt ttcagaagga
    28741 atcgatttga gaggggaccg attgatcggc agtatgatcg taggtgtagg tttgccgcag
    28801 atgaatcatg agcaggaact gatcaaatca tattatgatg agaaggaaca ccttggattt
    28861 gcttacgctt atcagcttcc tggaatgaac aaagtcttgc aagctggtgg tcgagtgatt
    28921 cgtggcatgg cggatcaagg aatcgttgta ttggcagatc aacgattttc tagtttttct
    28981 tatcgacgtt tattcccaca acattggcaa acagcaagag aagtacgaag tgttggggaa
    29041 ttggaagaag gaatacagcg tttttggtta tcaaaagaag cagtaaatca aaaaaacgag
    29101 gaatgacaga cgtcagcctc gttttttgat taacgatagt tagtgaattg caactcgatt
    29161 ggtaagtcaa gtttacgtaa taaagcaatc acagcttgta gatcatcacg gttcttacct
    29221 gtcactctaa tctgatcttc ttggatttgt gcttttactt tcagtttttc atttttgata
    29281 gctgtagtga ttttcttcgc gttttcacga tcgattccgt tgaccaaatc ggcagtttgt
    29341 cgagctttgc cacctagagc gtgttctgat tcggaaaaat ggatattttt gattggcaca
    29401 tttcttttga tcagcttacc aaataataca tctttgatct gttcgatttt gaaatcatca
    29461 tcgctcaata caactagatt gctgttctct aatttgattt ctactgtaga atccttgaag
    29521 tcaaagcgat tggacaattc ttttgttgtt tgctgaatcg cattttttac ttcttctgta
    29581 ttgacttctg atacaatgtc gaagctagca tcttttgcca taggaaccct cctgatatac
    29641 atataaatat ttaccataca tctatcttat cataggatta gggcagattc ttacctgttt
    29701 gctaaaattc ttctcatttg agttcgaagt attttatttt aatagataaa ctgttattct
    29761 tacatttgga ataccaaagt aggggagagg tgcttgtgag taaaattatg actgcgtatc
    29821 ataaaaatga aagattaaag aaaatagtgg ttattttgat aaccggattg accgctgcgg
    29881 tcgctttaaa ttattttttg attccagcca atgtcttttc tgcagggatg aatgggatcg
    29941 ctcaaatcat ttcctcgctt ttatatgaat ggtttcatat caagatagac acaggaacat
    30001 ttatttttct tttgaatatc cctgtgtttg tattagggtt tctcaaagta ggaaaacgag
    30061 caacgatcct tagttttgtg aatgttgtat gtgtatcggt gatgacgatg ctgcttccag
    30121 tcggacagat cactgataat attttgatga atgccctagt aggcggtgtt ttattaggtc
    30181 ttggcgtagg attatcttta aaaatgggct ttacaactgg tgggatggat atcatctctc
    30241 ttgtactttc acaaacaact ggaaaaacag tcggaaacta catgctgatt ctaaatggta
    30301 tcattgtagt catcgcaggc ttcttcttta actgggaaag tgccttgtat acgattattt
    30361 ccatttatgc aatgactcaa gtaattgatg ccatccacac gagtcatcaa aaagtaacag
    30421 caatgatcgt taccgtcaag ccagaagagg taacagaaga actagcaaaa cggatggtac
    30481 gtggaatgac attactgccg tctgtcggtg gctattcaaa aaaacaaggg aacatgatca
    30541 tgatggtcat cacccgctac gaaatgtatg atcttgatca gatcgttcat gaaatcgacg
    30601 acgatgcatt tatcaatatt ttaccaactc attcagtatt tggccggttt gcaaatgaaa
    30661 acgaacaaaa aatgtatcga acaactggcg tattgccaga aataaaaaat cgttcacaaa
    30721 aaaaatgagg aatcaggttt attttagaat aaatttaatc tattacatct aataagaaca
    30781 tttttataaa tttaaacgag aatcttactt ctaaattctc atttacaagc agctaaaaaa
    30841 agagtatgat aaataaaaat cgtgttaagg agtgtcgtac gatgagcgaa gcaaaccaag
    30901 aacggtctgg acaagaaata gaactaatca aagaacgtat tcttgctgcc ttggaaatgg
    30961 tgattgatcc tgaattagga atcgacatcg tcaatttagg cttgatttat gatatagaat
    31021 tcaatcctga aaatggagaa acagtcatta agatgactct aacaactatg ggctgtccat
    31081 tagcagatat tttaacagaa tctatccatg acgcattaaa agaagtgcca gaagtaacga
    31141 aagcagaagt caaactagtc tggtatccag catggacgac agataagatg agccgttatg
    31201 cacggattgc gttggggata cgatagaaat cttggctgac tagctatgag aagcaaatag
    31261 gaaaaacaaa aaagcctttt atcctttcta gaagaaaaga gataaaaggt ttctttgttt
    31321 ataaaatttt gtctgctata ttataagtta attaaataaa tgtgttctat tgataaagga
    31381 cattcaaatc cctttccttt tggaatcctt cttttttaca tcagtttgat tttcgtatag
    31441 tattctttct catcttcacg ataaatcgca gccccatttt tttgttgtgc tttaagactg
    31501 ccaagatttt ttttatttac agacaaaagt gcctcatcaa tgccgatttt ttttgcctct
    31561 tgtagtacta atgctaatcc ttgtgtggca tatccttttt tacgataatt aggggagatg
    31621 ccgtaaccaa tatggccagc gccgtttctt aatgtttcgt tcagtttatg gcgcaaatta
    31681 aaaattccta catactcatg ttcatcactt tctaaaataa agacggttgc gggaacgtaa
    31741 ctctcaggaa gatcgattcc taaagacgtt ttttcgcatt tttttacata atctaaaaat
    31801 tcagcaaatg tatagccata agcattgttg ataaatccac tctcatttgg tccaaagcta
    31861 gtttgtagct gataagcttt ctttaaatct gcttcccata gtttgatcag tttcatcgta
    31921 ttcacctctt taagtaacct tattcatttg ttttctacta tagcactatt gttgtaaata
    31981 gccatgccta tttttattta ttgtgtcata tcgtattaag aatcatcgaa caatacaaat
    32041 atgaattaca ataaaaaaat tatttgaagt tcgttcattc gttacttatg atctctagaa
    32101 gaaagaaaac gtgaattact tcagcgtcta gaattccaga aggatagtaa tcaacagaag
    32161 aaataatcaa tatccaaatg aaagaagata gctgaatcga gaaaaagagg aaaaaagaaa
    32221 tgaagaaaaa aataaaatag ttactaatcc cagagtcgtt cgtttaacat tgaaatcgat
    32281 catatggata aaacagattt tttcaccaac cctcaataga tgatggagtt gctcggatga
    32341 attatttgtg tagatggcat aagggagggc gtacagaaat gaaaaagaac aaaaatatgt
    32401 tcttgacaaa aaacacatgt aatcctaaac taaaagatgt gatgcggcta tggcggaatt
    32461 ggcagacgcg ctagcttcag gcgctagtga gggtgacctc gtggaagttc gagtcttctt
    32521 agccgcataa tttgtttttt tataaagaga aggtacacct ggagcgatga gaaagaaaat
    32581 aacatattat ctaaaagaat ataggaaaaa ttttaaacaa cggaaccaga ttcaaataaa
    32641 tcaaaaaaaa tttgtgtgaa ggtgggacag tttaaaacct aatagattga ttcaaatagt
    32701 agatttatag attctgatac aagaaatact ggcaaacgat agttaaggaa taaagactaa
    32761 acttaaactc aaaaagaggt catgcgaaag aataaatcgc atgacctctt actatatttt
    32821 gaatgtgttt aaacgttcaa ataatgaatc ttagtaccaa ccgtttgctt cccagaatgc
    32881 tttcgccgct tcccaagaac cataacgtga agtaacatat tgttctgcta ctctttcttg
    32941 gtttgcagca gagtagtcac catttaagta agaagaatct aattggtaac ggccgatgta
    33001 acgtccgttc gttgctgtgt aagaaccgct tgattctttt tgtgcgatcc attcttttgc
    33061 agagcttgta cttgctggag tagcagcagt ttctgttaca gcaggagcag gttgtgtttc
    33121 agcaacaggc gctgcttgtg tttccgtttg aaccgtttct tgtacaacag gttgttcagc
    33181 ttgaacaggt tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc
    33241 ttgtgcagag ccttctgttg ggattgttaa ttgttgtcct gagta
//
LOCUS       DAZUMV010000018        32468 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000018 DAZUMV010000000
VERSION     DAZUMV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.018"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..1948
                     /locus_tag="ACG8FK_000776"
     CDS             50..1948
                     /locus_tag="ACG8FK_000776"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS5096406.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2247..2966)
                     /locus_tag="ACG8FK_000777"
     CDS             complement(2247..2966)
                     /locus_tag="ACG8FK_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS5096407.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    3156..3203
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            3303..3635
                     /locus_tag="ACG8FK_000778"
     CDS             3303..3635
                     /locus_tag="ACG8FK_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096408.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3622..5625
                     /locus_tag="ACG8FK_000779"
     CDS             3622..5625
                     /locus_tag="ACG8FK_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS5096409.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5644..6114
                     /locus_tag="ACG8FK_000780"
     CDS             5644..6114
                     /locus_tag="ACG8FK_000780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS5096410.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6135..6719
                     /locus_tag="ACG8FK_000781"
     CDS             6135..6719
                     /locus_tag="ACG8FK_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFS5096411.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6734..7720
                     /locus_tag="ACG8FK_000782"
     CDS             6734..7720
                     /locus_tag="ACG8FK_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS5096412.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7710..8021
                     /locus_tag="ACG8FK_000783"
     CDS             7710..8021
                     /locus_tag="ACG8FK_000783"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS5096413.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8043..9824
                     /locus_tag="ACG8FK_000784"
     CDS             8043..9824
                     /locus_tag="ACG8FK_000784"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS5096414.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9817..11193
                     /locus_tag="ACG8FK_000785"
     CDS             9817..11193
                     /locus_tag="ACG8FK_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS5096415.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLLERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11200..11835
                     /locus_tag="ACG8FK_000786"
     CDS             11200..11835
                     /locus_tag="ACG8FK_000786"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS5096416.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11865..13220
                     /locus_tag="ACG8FK_000787"
     CDS             11865..13220
                     /locus_tag="ACG8FK_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5096417.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13412..14806)
                     /locus_tag="ACG8FK_000788"
     CDS             complement(13412..14806)
                     /locus_tag="ACG8FK_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5096418.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14898..15869
                     /locus_tag="ACG8FK_000789"
     CDS             14898..15869
                     /locus_tag="ACG8FK_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS5096419.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15866..16648
                     /locus_tag="ACG8FK_000790"
     CDS             15866..16648
                     /locus_tag="ACG8FK_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS5096420.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16817..17188)
                     /locus_tag="ACG8FK_000791"
     CDS             complement(16817..17188)
                     /locus_tag="ACG8FK_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5096421.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17322..17765
                     /locus_tag="ACG8FK_000792"
     CDS             17322..17765
                     /locus_tag="ACG8FK_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5096422.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17808..18617)
                     /gene="yidA"
                     /locus_tag="ACG8FK_000793"
     CDS             complement(17808..18617)
                     /gene="yidA"
                     /locus_tag="ACG8FK_000793"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS5096423.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18636..20003)
                     /locus_tag="ACG8FK_000794"
     CDS             complement(18636..20003)
                     /locus_tag="ACG8FK_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5096424.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19987..20817)
                     /locus_tag="ACG8FK_000795"
     CDS             complement(19987..20817)
                     /locus_tag="ACG8FK_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS5096425.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20980..21417
                     /locus_tag="ACG8FK_000796"
     CDS             20980..21417
                     /locus_tag="ACG8FK_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS5096426.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21493..22113
                     /gene="rpoE"
                     /locus_tag="ACG8FK_000797"
     CDS             21493..22113
                     /gene="rpoE"
                     /locus_tag="ACG8FK_000797"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS5096427.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            22302..22808
                     /locus_tag="ACG8FK_000798"
     CDS             22302..22808
                     /locus_tag="ACG8FK_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5096428.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            22825..23724
                     /locus_tag="ACG8FK_000799"
     CDS             22825..23724
                     /locus_tag="ACG8FK_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS5096429.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            23811..24695
                     /locus_tag="ACG8FK_000800"
     CDS             23811..24695
                     /locus_tag="ACG8FK_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5096430.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(24887..24958)
                     /locus_tag="ACG8FK_000801"
     tRNA            complement(24887..24958)
                     /locus_tag="ACG8FK_000801"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24923..24925),aa:Arg,seq:ccg)
     gene            25018..26352
                     /gene="rpoN"
                     /locus_tag="ACG8FK_000802"
     CDS             25018..26352
                     /gene="rpoN"
                     /locus_tag="ACG8FK_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS5096431.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            26501..27592
                     /locus_tag="ACG8FK_000803"
     CDS             26501..27592
                     /locus_tag="ACG8FK_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS5096432.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            27633..28634
                     /gene="gap"
                     /locus_tag="ACG8FK_000804"
     CDS             27633..28634
                     /gene="gap"
                     /locus_tag="ACG8FK_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5096433.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            28744..29934
                     /gene="pgk"
                     /locus_tag="ACG8FK_000805"
     CDS             28744..29934
                     /gene="pgk"
                     /locus_tag="ACG8FK_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS5096434.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            30038..30793
                     /gene="tpiA"
                     /locus_tag="ACG8FK_000806"
     CDS             30038..30793
                     /gene="tpiA"
                     /locus_tag="ACG8FK_000806"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS5096435.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            30909..32207
                     /gene="eno"
                     /locus_tag="ACG8FK_000807"
     CDS             30909..32207
                     /gene="eno"
                     /locus_tag="ACG8FK_000807"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS5096436.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        10720 a         5747 c         6913 g         9088 t
ORIGIN      
        1 gcataacaac tttataatgt tgagtatacc aatttttgga ggcgttttaa tggaccgcta
       61 tttagattta ctttcagaga agttccctac aacggaagat gtcatcacgg aaatcatcaa
      121 tctcgaagca attttgcagc ttccaaaagg aacagagtta tttttgagcg atatacatgg
      181 cgaatttcct gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat
      241 caaagacctt ttctctgaac aaatgacgga aacggagatc agccaattca ctatttttac
      301 ggcttatcca gaatatgtgt tgacgactga ttggtacaaa aaacaagata aatcccagct
      361 gatccatcgt ttgatcgatc ttttacggtt tacgacagtc aaatacactc gatcaaaagt
      421 tcgaaaagga ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga
      481 tcgtatcaac gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg tcttgatgaa
      541 ggaagaagaa aaatttttaa caaaacttgc gcagacgatc caaaaatctg tcatcgatca
      601 tttgcatatc gtgggtgata tttttgatcg aggaactcaa gcagccaaag taatggatcg
      661 aatcatggat ttttcctctg tagatatcca gtgggggaat catgatattt tgtggattgg
      721 tgcgtattgc ggctctgagg catgtttatt gacgctttta cggattgccg cacgctacaa
      781 ctatttattt gaattagaga aagaatatgg attaaattta cgtccattat tttcttttgc
      841 ccatcaaacc tatcagaaga atccagtatt tacccctaaa aacaggaaag atctatctga
      901 aagagagcaa gaagaattag aaaagatcca tcaagcactt tctatcctcc aattcaaatc
      961 agaacatcag ttgttgaatc gacgacctga atttaaaatg gacgatcgta aattgttgga
     1021 aaaaatcaac tatgaggaat caacgatcgt tttagaaggt cagctctatg agttgaaagg
     1081 gacttgtttt caaacgatcc agccagatga tcctaaaaaa ttcttggaag aggaacaaaa
     1141 agtgatcgat agtttgatgt attcattcca gcactcgtta agaatgcaaa aacacatgac
     1201 gtttttgatc gaaaaagggg gaatgtatct cacgaacaat cagcatgtac tgttccatgg
     1261 ctgtatacct gtagatgaaa aggggcagct cttagcattt gaattagacc aatcttatcg
     1321 cggaaaagaa ctgctgggat tttttgaaaa acagataact gaaagcgcaa aagatttgac
     1381 tgcaaatgaa gatttggcga ccgatttgat ttggtatgcg tggagcgggc cattttcgcc
     1441 tttattcggc aagagtaaaa tggcaacttt tgagcgctat tttctcacag agcctcatac
     1501 gcacgtcgaa aaaagcaatc cctattatca cttgcgagat gaagagtggt tcagtgaaaa
     1561 aattttggca gaattcgatg caaaagaaga aggttcagca atcatcaatg gacacacgcc
     1621 agtaaaagta aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat ttgttattga
     1681 cggaggtcta tccaaagctt atcaaaaaac aaccgggatt gctggctact cattactatg
     1741 caattcttac ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg aacaacaatt
     1801 tgcaaaaaaa tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa
     1861 acttaatcgg aatacgacaa aaggaatgga attacagcaa cagattactc aactccaaag
     1921 gctgttggat tatcgaaagg acgattgaga aaaatgctag ccaacattgc tcaatagact
     1981 aaaaagcgac tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg
     2041 taaaatatta gaagctattt ttctgtttat ttctctaata ctcaggtcag ggcgctgatt
     2101 tcacgacctc ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
     2161 ccaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca
     2221 ggctcacgac ctaatcagaa aaacgattaa aattcattca caaagaaatc gaataaacgc
     2281 tgttcagatg aatctacacg atgagaaagt tccggatgcc attggacacc aaggattttg
     2341 tgcatattat ctttactttg gactgcttca atgagtccat cgttggaaaa ggcgatggct
     2401 tttaacaaag gagaaagatc tttgactgcc tgatgatgat aggaattcac aaagtatgaa
     2461 tcagggagaa ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg agttgcaaac
     2521 tgcggggcag tgggctgttg ttcgtgtttt acggaccatt ttggatataa tgaaagatct
     2581 tgataaagtg ttccctccaa cacgacattc aatagttgaa gtcctcggca gactgcaaaa
     2641 atcggttttc tttgtttcaa tgcttccaag atcaatgcct gttcgaattg gtctcgttga
     2701 atatttgttt ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg actaacatcc
     2761 tgaccgcctg caagaatgag tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga
     2821 gacatgactg gcagaataag aggtaatcct tgagcatcta aaactgcatc cacaaatccc
     2881 tgcggtgtat agcttacttg atttccttgg aaagtatctg tcgctcgaat cagttgattc
     2941 ccggcaatgc cgatgattgg tttcattttt attcccccta aagtagacta attgtaacaa
     3001 ggatttaaaa aaatttaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg
     3061 ttactatctg taaagagaat tttcgtgtga aattcatgac gatagttgaa aatattgctt
     3121 aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct
     3181 agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct
     3241 ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg
     3301 aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaga
     3361 agcgtgctcg aaccctacgg cagcagttga agacctatgc agaagaaaaa gagcaagaat
     3421 tggagataat ccaaacgaac agtcggcaac agctgaaaca gttggtagaa gaaaaagaac
     3481 aattcgaaaa gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg
     3541 tgcaagcaac tttgaaagac caatatcaaa agcatcagag tcaagcaatc ggtgcaatca
     3601 ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca
     3661 gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa tgttgaaatc
     3721 cgggatctgt atcaaaatag aacaaacaac cagtgggtag aaaaatattt tccagatacc
     3781 acgattattg ataaagaagc aaaattaagc gctttaggca atcggctgaa agacatccaa
     3841 gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa
     3901 cgtcaagaac tttccttaca agacttggaa gcaaattatt ccgaagaaaa tttcatgaat
     3961 aaattagaag aagtccttga tttaaaagaa aagtgggaac agctaactga aaaacgtgat
     4021 cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt
     4081 atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa
     4141 agtttcaagg aagaactaag taagcttgat gacgtttata tggaagaaat agattatacg
     4201 ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgc tatagaagaa
     4261 attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa
     4321 aaattgcaag aaaccaaaca gcaattatcc attgttcacg agcagcaaaa aaagataacg
     4381 acagcgttag gtcagtgcag cggctatatc caagaatttg aatgggtaga agaagtcaca
     4441 cttgccgttg cagaacggga aaaagtaaaa gaacggttta tccatgccac ttatctgatc
     4501 gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa
     4561 actgtatcag aagaagatat atacgttagt ttcgaaacac caaactcaga agagattcag
     4621 caggaagtcc ctactaaatt gaaaaaccat ccgcttatcg aaccttttga attgctgact
     4681 gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa cgccttggat gacaccattt
     4741 tatcttgtct tcttcgggat gatggtcgca gatgttggtt acggattgct gatgctcatt
     4801 gggacaatac tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa
     4861 ttctttgaga ttctctcggt accatcgatc atctggggat tgatctacag ttcatttttc
     4921 ggacaggcat taccaaaaga actatttggt atccgcttgc ctttcccaat cctatctacg
     4981 acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat acagatcttg
     5041 gttggattat tcgtggcagc aaaggaaaat atcaggagga aagattactt gagtgctgtc
     5101 agtgatggtt ttgcctggca agggatcctg attgggatcg ttattgcatt gattggtgtc
     5161 atgttgataa aaaatacagc atttgtttat cttggcgcgg ggctatcgat cattgctgct
     5221 ttatgtatct taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa
     5281 ggagcgtata atctttacgg attgactggt tatatcggtg atttagtcag ttatacacgt
     5341 ttgatggcac taggtatttc aggcggaagt attggcgcgg cattcaatat gctagttgct
     5401 ttcatgccgc cagccgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca
     5461 ctgaatatgt ttttgacact gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc
     5521 gaattttttg gaaaatttta tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg
     5581 gaaaaatacg tcaacatcaa tcataagaaa aaaaacaata aataattgga ggatttttat
     5641 acaatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcagt attagcgatg
     5701 gcaacagcga cgattttttc agggattggt tcagctaaag gtgttggaat gacaggtgag
     5761 gcagcagcag cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa
     5821 ttgcttccag gtacgcaagg gttgtatgga ttcgttatcg cctttttgat ttttatcaac
     5881 ttaggaaatg atatgtcagt tgtacaagga ttgaactttc taggcgcttc tctgccgatt
     5941 gcttttacag gtttgttttc aggtatcgcc caagggaaag ttgcagcagc aggtatccaa
     6001 attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa
     6061 acatatgcga ttttaggttt cgttatttcc ttcctattag tattgaatgc ctaagcataa
     6121 aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaat gaagcagcag
     6181 agcaagaaag agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa aactttgaac
     6241 aaaaacgtac gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac
     6301 ttgaaaaaaa ataccgtcag cttaggaatc gccaacaagt agaagtacga caagaaaatt
     6361 tgaatgcgaa gcaggaattt ttacgacgat tgtttgcaga tgcaatagct gaaatggaga
     6421 attgggatga atctgaacaa atccagttta tcaaaaattc attgtattca ttacctttga
     6481 caggcaaagt aacattcatt gctggagaaa aatcagcagc ttatctttct cagacattgc
     6541 tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg
     6601 atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc
     6661 ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag
     6721 gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga
     6781 acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg
     6841 tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt
     6901 cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc
     6961 ggagcctgaa atcgtttgga tctatacgat gcgctatact ttccataatc tgaaggtttt
     7021 gacaaaagcc gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta
     7081 ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac tccctgaaag
     7141 cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg
     7201 aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct
     7261 aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac
     7321 gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag
     7381 ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta
     7441 cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg
     7501 aaatcttgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca
     7561 agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga
     7621 ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaaccaga
     7681 aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca
     7741 aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc
     7801 aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca
     7861 cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac
     7921 ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc
     7981 aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa
     8041 ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt
     8101 ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta
     8161 tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc
     8221 caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca
     8281 tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa
     8341 gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt
     8401 tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag
     8461 aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca
     8521 aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa
     8581 atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca
     8641 aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct
     8701 tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag
     8761 tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg
     8821 gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta
     8881 atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag
     8941 tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca
     9001 tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg
     9061 agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt
     9121 cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc
     9181 gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac
     9241 cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag
     9301 cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag
     9361 aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg
     9421 gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg
     9481 attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt
     9541 atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca
     9601 aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg
     9661 cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca
     9721 aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa
     9781 cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc
     9841 ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa
     9901 ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa
     9961 gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct
    10021 tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga
    10081 gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat
    10141 ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc
    10201 caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca
    10261 gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca
    10321 acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt
    10381 gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc
    10441 atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct
    10501 ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca
    10561 gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca
    10621 ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt agaacgagca
    10681 ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa
    10741 gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc
    10801 ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt
    10861 tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg
    10921 atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt
    10981 ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt
    11041 gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat
    11101 ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac
    11161 atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat
    11221 ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat
    11281 aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag
    11341 aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta
    11401 ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa
    11461 aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat
    11521 tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt
    11581 gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg
    11641 acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga
    11701 gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt
    11761 atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg
    11821 ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca
    11881 aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga
    11941 ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg catgggacaa
    12001 attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat
    12061 tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga
    12121 tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag
    12181 tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg
    12241 tgatgaagct catgctttac atcctaaaat tggcctttgt tatccaattg attggtacgg
    12301 ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt
    12361 tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag
    12421 ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg
    12481 ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga
    12541 ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt
    12601 tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg
    12661 caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc
    12721 tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat
    12781 ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc
    12841 gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg
    12901 cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca
    12961 tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg
    13021 aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg
    13081 caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg
    13141 tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag
    13201 aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa
    13261 gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt
    13321 tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca
    13381 ataagatttg attcctttat ttatcaatag gttataattc atattttttt attttttctt
    13441 ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa
    13501 cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt
    13561 tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc
    13621 gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat
    13681 tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga
    13741 ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg
    13801 cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag
    13861 aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga
    13921 aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa
    13981 taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga
    14041 tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt
    14101 ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa
    14161 cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg
    14221 ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa
    14281 caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat
    14341 ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa
    14401 caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt
    14461 caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg
    14521 tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca
    14581 aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat
    14641 aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa
    14701 tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt
    14761 ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt
    14821 aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt taatcttttg
    14881 tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt
    14941 ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca
    15001 gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga
    15061 tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc
    15121 cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc
    15181 aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg
    15241 ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa
    15301 ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca
    15361 ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta
    15421 aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc
    15481 cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg
    15541 taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa
    15601 gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag
    15661 aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg
    15721 gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga
    15781 cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata
    15841 acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc
    15901 agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga
    15961 aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa
    16021 gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg
    16081 tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac
    16141 gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta
    16201 catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc
    16261 gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga
    16321 tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg
    16381 aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt
    16441 tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc
    16501 ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg
    16561 cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt
    16621 gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg
    16681 gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt
    16741 aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta
    16801 aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga
    16861 caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccgg
    16921 cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat
    16981 agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt
    17041 catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc
    17101 ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca
    17161 ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc
    17221 ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt
    17281 aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg
    17341 gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa
    17401 gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg
    17461 aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg
    17521 gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt
    17581 atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta
    17641 taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg
    17701 tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa
    17761 actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat
    17821 tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct
    17881 tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg
    17941 gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc
    18001 tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa
    18061 tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt
    18121 gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt
    18181 gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc
    18241 gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga
    18301 tgactgccga cacggcgtgc catcacttcg atctctagga aatcttcatg tgtcagtccg
    18361 tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata
    18421 acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag
    18481 ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct
    18541 tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg
    18601 actagtttga tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca
    18661 ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc
    18721 aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc
    18781 gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc
    18841 aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga
    18901 tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac
    18961 aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt
    19021 ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac
    19081 cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta
    19141 aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct
    19201 tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg
    19261 gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc
    19321 actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt
    19381 aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc
    19441 aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg
    19501 gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta
    19561 agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga
    19621 ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac
    19681 agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt
    19741 tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct
    19801 aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat
    19861 tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga
    19921 tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt
    19981 tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct
    20041 ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc
    20101 ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc
    20161 ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac
    20221 tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat
    20281 gacttgcccg cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc
    20341 atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata
    20401 agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc
    20461 gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc
    20521 actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc
    20581 acctgaattt ctgatcactg gagcgtaacc tgcatctttc agtgtttgga tcccgtcact
    20641 tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa
    20701 atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa
    20761 aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca
    20821 accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt
    20881 gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact
    20941 aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca
    21001 ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat
    21061 tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag
    21121 attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat
    21181 gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg
    21241 ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa
    21301 ctgcatgtta gtttgaaaga ccgtcccaca ggagggaagg ttcgaatcga ttatgatctg
    21361 ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata
    21421 taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag
    21481 gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg
    21541 atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta
    21601 gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca
    21661 caattttata ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg
    21721 ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac
    21781 gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat
    21841 gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa
    21901 gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat
    21961 aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca
    22021 ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa
    22081 gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga
    22141 cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt
    22201 tttgtatata aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc
    22261 accttgtacg tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata
    22321 agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt
    22381 atcaaaaaat gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg
    22441 tgatgcaaga tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc
    22501 attttagtgc atggttttat cggatattga tccgcacggc attagatagt tggcgcagac
    22561 aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag
    22621 aaacttccgt tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga
    22681 ttctgatcct ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata
    22741 tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc
    22801 tagaatgaag gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag
    22861 aaaacttata aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt
    22921 attatcgaag agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc
    22981 ggagtatctt tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt
    23041 cgaagctttg cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa
    23101 cgctttactg atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat
    23161 gagaaagaaa ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca
    23221 gattttgaaa aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg
    23281 ggagattatc agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc
    23341 caaacagcag cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt
    23401 gtccagctct cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa
    23461 gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact
    23521 gagcaagata ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt
    23581 ttttatttga acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct
    23641 ggttatatgg gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca
    23701 tcggaagatt atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca
    23761 agtaagtaca agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa
    23821 attatttagt tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg
    23881 tggcctcctt gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat
    23941 ctttcaaaca atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca
    24001 aagaagccat tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag
    24061 acagtacaaa aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg
    24121 gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg
    24181 ccactttttt cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag
    24241 ctgaagcagg gcacgagatt ggcaatcaca catacgatca tcagaatctg gccactcttt
    24301 ctgcccagtc gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg
    24361 gcaaaacccc cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga
    24421 tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc
    24481 ctgatttgat tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc
    24541 atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc
    24601 aagggtatcg catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact
    24661 attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata
    24721 acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc
    24781 tatacaggta caacaaaaaa ctgtccacca attttctttg gcggacagtt ttcacttaaa
    24841 aataaagaag caactattcg taaattatgc taaaatagca gtagaacgcg tcctgaggga
    24901 atcgaacccc cgtctcaaga accggaatct tacgtgatat ccactacact aaggacgcaa
    24961 gcactgggat aattgtaact aaacagataa aaaattacaa ggggattgga caaaaaaatg
    25021 aaatttgagc aaaatttttc acaaaaacag cagcagacac agaaactagc catgacgcaa
    25081 cagttgcaac agtccatcca agttttacaa tataacagtg aggagctgtt ggcattcgtt
    25141 gagaatcaag caatggaaaa tccattggtg gaagttgttg agccggaatg gcagccagat
    25201 tacataaaag cttcttcatc ttcttatgaa ggagaagaga caaattacct aaatcagatt
    25261 cctgatacaa aagggtcttt atttgattca ttgatcgaac aagtccattt gaattaccga
    25321 gatacttttt tgaggaaaat cgttctttat cttgtagaat acatcgattt gaacggattt
    25381 ttgacgatta gtttagaaga agccggaaaa gagattggag ctacgcctat ccaaatgctc
    25441 gatgcgttga ctctgattca gcaattagaa ccagctggag taggtgcacg tagtttgcaa
    25501 gaatgtctca tgcttcaaac agaacgagat gattatgcgc cggaattagc ttatatcgtg
    25561 ttggaagaat gttttgagga actggtggag cgaaaatgga aagaaatcgc acaacgtttt
    25621 tctgttgatc tacatgctgt tcagcagata ttcgactatt tacaaacgtt gagtccaagc
    25681 cctggccgga ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta
    25741 gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc
    25801 tttcaagaga gttacttcaa acaaatgtca caacaagcag ataaagaaac tgaaaattat
    25861 ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca acgtggggat
    25921 acaattttgc gggttgctca agtcattgtt tcccggcaaa aagctttctt tatcgacaag
    25981 gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa
    26041 tccacggtca gtcgtgcagt caacgggaaa tatttggaaa cagacttcgg tgtatttgaa
    26101 ttaaaaaaat ttttcactac acgaattcct acgaatagta cagaacagac agaagacctt
    26161 tcagcagata cagcgaagaa aaagctgcag gaattagtcg atcaagaaga caaaaacaag
    26221 ccgttgtctg accagaaatt ggttgaattg ctgaaaaaag atgagatagc gatctcaaga
    26281 cgaacagtag caaaatatcg tgatttatta ggaatcccga gttcatcaaa aagaaaacgt
    26341 tatgataact aatcagtcaa aagactagaa aaagtatttc actttttcta gtctttttag
    26401 ttgaaaaacg agagcaaaac tgttagactt acagtcgtga ggttgagatt tccctttatt
    26461 gatgtgaaat tcgaatagtt aatttctttt tttatcgtat atgggtcgtt ttaagtctac
    26521 attggacgta aagtgtccaa ctaaggagat cgctatgctg gatgaaataa aaatgattga
    26581 atctgttgca ccagatatta tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat
    26641 ctattggatg cagccgattg ggcgtcgaag tttatcggaa agtatgggga tcacagaacg
    26701 agtcttacga acagaaacag accttttgaa aaatctgaat ttgatcgaca cctcaaaaag
    26761 cggtatgacg ctgacaacta agggcgaaga agtttatcaa agccttgaaa acttcatgga
    26821 tcagctttta ggtacgcatc aaaccgaaca acagttggca gaatattttg gtaaccaacg
    26881 ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg cattcggcga
    26941 agcgctgagc gaagccttag accgactact tccagaaggc gagaatatca tcgctgtgat
    27001 gggaggaact actatggctg tggtagccga acaattgtct aatctggaaa ataagaaacg
    27061 gcataacctg tttgtccctg caagaggcgg gattggtgaa gcgataactg tacaggcaaa
    27121 ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac caccgcgcac tttatgttcc
    27181 ggaacaatta agtctggcaa cttacaattc tttactcaat gagccatcta tccaagaagt
    27241 cttaaacttg attagtgaag cgaattgtgt tattcatagt attgggcgtg cattacacat
    27301 ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt
    27361 tgcggaatct tttggctact ttttcaatga aaaaggtgaa gtcgtttata aggtccctag
    27421 aatcggcata gaattgcaag atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg
    27481 aaaatcaaaa gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct
    27541 catcactgat gaagctgctg caaatgagat tttaaaaggg gtaacccttt aaaataaata
    27601 tttttttgat tttcataagg aggaaatctt aaatgacagt taaagtaggt attaatggtt
    27661 ttggacgtat tggacgtcta gcattccgtc gtatccaaga tgtagatgga atcgaagtag
    27721 tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa
    27781 ctcaaggacg tttcaacgga acagttgaag ttcatgaagg ttcattcaac gtaaacggaa
    27841 aagaagttaa agtattagct aaccgcaacc cagaagaatt accatggggc gaactaggcg
    27901 tagatatcgt tctagaatgt actggtttct tcacttctaa atcagcagct gaaaaacatt
    27961 taacagctgg tgctaaacgc gttgttatct ctgctcctgg cggaaacgat gttccaacaa
    28021 tcgtttataa cacaaaccac gaaacattaa ctggtaaaga aacagttatc tcaggtgctt
    28081 catgtactac taactgttta gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg
    28141 ttgaaggatt aatgacaact atccacgctt acacaggtga ccaaatgact ctagacggac
    28201 ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact
    28261 caacaggtgc tgctaaagct atcggtttag taattcccga attgaacggt aaattagacg
    28321 gagctgctca acgtgttcct gtaccaacag gttcattaac agaattagta acagttcttg
    28381 aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa agaagcttca aacgaatctt
    28441 acggatacaa cacagacgaa atcgtttctt cagatatcgt tggtatgact tacggttcat
    28501 tatttgatgc tacacaaact aaagtaatga cagttggcga caaacaatta gttaaaactg
    28561 ttgcttggta cgacaacgaa atgtcataca ctgcacaatt agtacgtact ttagaatact
    28621 tcgctaactt ataagataga caaacgtaca agtgtaaatt atagacatgt acatttaaaa
    28681 gcggggaagc aatcgcgctt cttccgcttt ttttgataat aagtttattc aggaggaaat
    28741 aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt
    28801 gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca
    28861 gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac
    28921 cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa
    28981 cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa
    29041 ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga
    29101 tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg
    29161 gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct
    29221 tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa
    29281 gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt
    29341 ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat
    29401 aaaatcttga tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc
    29461 ggtaactctc tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca
    29521 ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt
    29581 gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga catcggccca
    29641 gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga
    29701 cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca
    29761 atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc cgcagcagca
    29821 gaacaattag gatttgctga caaattcaca cacatctcaa caggtggcgg cgcaagcttg
    29881 gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc
    29941 aagtttgctc aattctgacg agtaatcgaa caaaaggtga agggagtttt catgactccc
    30001 tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg
    30061 taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa
    30121 caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca
    30181 agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg
    30241 ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt
    30301 tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga
    30361 catcaataaa aaagcgaaag ctatctttgc aaatggtatg acaccaatcc tatgctgcgg
    30421 cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac
    30481 agctggttta gctgatcttt cagctgaaca ggtgtcaaac atggttattg cttatgaacc
    30541 aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg
    30601 agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat
    30661 ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt
    30721 tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt cattgttaga
    30781 cgctgtaaaa taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc
    30841 aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga
    30901 gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta
    30961 acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc
    31021 cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc
    31081 gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag
    31141 caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag
    31201 acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg
    31261 ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta ggcggattca
    31321 acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata
    31381 actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag
    31441 ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat
    31501 tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag
    31561 gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg
    31621 ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac
    31681 tagctgactc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat
    31741 tagtttctaa atatccaatc atctcaatcg aagatggatt agacgaaaac gactgggacg
    31801 gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt
    31861 tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc
    31921 ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta
    31981 aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa
    32041 tctctgatat cgctgttgca acaaacgctg gccaaatcaa aactggttct ctatcacgta
    32101 ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg
    32161 cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg
    32221 aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa
    32281 ctctctactt gctgaaatat cagcaggtag agagtttttt tgcaactagc ttctgtatat
    32341 gatgtttaca aagaatggta caagaaaaga tcatccttga aaaaggaaag ctaacaataa
    32401 ctagcatcat ataagagctt ttaaggatag aacagtagaa taggaatcta tgggcaagtc
    32461 ctctcgta
//
LOCUS       DAZUMV010000019        30922 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000019 DAZUMV010000000
VERSION     DAZUMV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.019"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            176..574
                     /locus_tag="ACG8FK_000808"
     CDS             176..574
                     /locus_tag="ACG8FK_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5096437.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            811..1353
                     /locus_tag="ACG8FK_000809"
     CDS             811..1353
                     /locus_tag="ACG8FK_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5096438.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1436..1987)
                     /gene="lepB"
                     /locus_tag="ACG8FK_000810"
     CDS             complement(1436..1987)
                     /gene="lepB"
                     /locus_tag="ACG8FK_000810"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5096439.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(2139..2366)
                     /locus_tag="ACG8FK_000811"
     CDS             complement(2139..2366)
                     /locus_tag="ACG8FK_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS5096440.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2363..2881)
                     /gene="msrA"
                     /locus_tag="ACG8FK_000812"
     CDS             complement(2363..2881)
                     /gene="msrA"
                     /locus_tag="ACG8FK_000812"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5096441.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2901..3614)
                     /locus_tag="ACG8FK_000813"
     CDS             complement(2901..3614)
                     /locus_tag="ACG8FK_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS5096442.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3617..4513)
                     /locus_tag="ACG8FK_000814"
     CDS             complement(3617..4513)
                     /locus_tag="ACG8FK_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5096443.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4671..5513)
                     /locus_tag="ACG8FK_000815"
     CDS             complement(4671..5513)
                     /locus_tag="ACG8FK_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5096444.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5645..6298
                     /locus_tag="ACG8FK_000816"
     CDS             5645..6298
                     /locus_tag="ACG8FK_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS5096445.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6361..6888)
                     /locus_tag="ACG8FK_000817"
     CDS             complement(6361..6888)
                     /locus_tag="ACG8FK_000817"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5096446.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6908..7855)
                     /locus_tag="ACG8FK_000818"
     CDS             complement(6908..7855)
                     /locus_tag="ACG8FK_000818"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS5096447.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7867..9750)
                     /locus_tag="ACG8FK_000819"
     CDS             complement(7867..9750)
                     /locus_tag="ACG8FK_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5096448.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(9913..11991)
                     /locus_tag="ACG8FK_000820"
     CDS             complement(9913..11991)
                     /locus_tag="ACG8FK_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5096449.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(12004..12279)
                     /locus_tag="ACG8FK_000821"
     CDS             complement(12004..12279)
                     /locus_tag="ACG8FK_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096450.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12641..13096)
                     /locus_tag="ACG8FK_000822"
     CDS             complement(12641..13096)
                     /locus_tag="ACG8FK_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096451.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(13250..14458)
                     /locus_tag="ACG8FK_000823"
     CDS             complement(13250..14458)
                     /locus_tag="ACG8FK_000823"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS5096452.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14570..14902)
                     /locus_tag="ACG8FK_000824"
     CDS             complement(14570..14902)
                     /locus_tag="ACG8FK_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5096453.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            15050..15925
                     /locus_tag="ACG8FK_000825"
     CDS             15050..15925
                     /locus_tag="ACG8FK_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5096454.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(16007..16588)
                     /locus_tag="ACG8FK_000826"
     CDS             complement(16007..16588)
                     /locus_tag="ACG8FK_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS5096455.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16594..17853)
                     /locus_tag="ACG8FK_000827"
     CDS             complement(16594..17853)
                     /locus_tag="ACG8FK_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5096456.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17855..18901)
                     /locus_tag="ACG8FK_000828"
     CDS             complement(17855..18901)
                     /locus_tag="ACG8FK_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096457.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(19136..19411)
                     /locus_tag="ACG8FK_000829"
     CDS             complement(19136..19411)
                     /locus_tag="ACG8FK_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS5096458.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19654..20964)
                     /gene="der"
                     /locus_tag="ACG8FK_000830"
     CDS             complement(19654..20964)
                     /gene="der"
                     /locus_tag="ACG8FK_000830"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS5096459.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(21146..22375)
                     /gene="rpsA"
                     /locus_tag="ACG8FK_000831"
     CDS             complement(21146..22375)
                     /gene="rpsA"
                     /locus_tag="ACG8FK_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS5096460.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22510..23190)
                     /gene="cmk"
                     /locus_tag="ACG8FK_000832"
     CDS             complement(22510..23190)
                     /gene="cmk"
                     /locus_tag="ACG8FK_000832"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS5096461.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(23239..23874)
                     /locus_tag="ACG8FK_000833"
     CDS             complement(23239..23874)
                     /locus_tag="ACG8FK_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS5096462.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23940..25343)
                     /locus_tag="ACG8FK_000834"
     CDS             complement(23940..25343)
                     /locus_tag="ACG8FK_000834"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS5096463.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(25330..26361)
                     /locus_tag="ACG8FK_000835"
     CDS             complement(25330..26361)
                     /locus_tag="ACG8FK_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5096464.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26392..26613
                     /locus_tag="ACG8FK_000836"
     CDS             26392..26613
                     /locus_tag="ACG8FK_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS5096465.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26778..27515)
                     /locus_tag="ACG8FK_000837"
     CDS             complement(26778..27515)
                     /locus_tag="ACG8FK_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5096466.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLILYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27668..29008)
                     /locus_tag="ACG8FK_000838"
     CDS             complement(27668..29008)
                     /locus_tag="ACG8FK_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5096467.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(29245..30768)
                     /locus_tag="ACG8FK_000839"
     CDS             complement(29245..30768)
                     /locus_tag="ACG8FK_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5096468.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         8713 a         6424 c         5241 g        10544 t
ORIGIN      
        1 taaagaaatt ccatctatta gttaagtgaa agtgaaccag tcctcattct atcacatctt
       61 cacttttttg atgatgcaaa tttttatttt ggtttctata tatgaatcta aatttttgac
      121 agcgtttcct aaataagaga tgatagacga acaaaacaga aaggagacga aacaaatgaa
      181 tagtatggtt tttgttaatt ttcctgttgc tgatgtcaat cgttccgttg ctttttatga
      241 aaagctgggt tttaaaaaaa atgaagaatt ttctactgaa gaagctggtt caatggtatg
      301 ggatgacact ttttggataa tgcttctctc tcatgatttt tataaaaaat ttataaaaga
      361 taaagaaata gctgataata aaaaaacaag tggtgctttg atttcgttta gtgttgaaaa
      421 tgcggatgaa gtaaaaaaat ttgctcaaat tgcaaaagaa aatggtggta ctttccacga
      481 agtagatatg ggaatgcctg aagaagaaat gttttcatta gaagtccaag atctagacgg
      541 aaatacatta gaacccgtat ggatgaagat gtaagcagat aactaatctg acgtttttta
      601 taacaaaaaa acaaaggcgg atgatacgat aagtattctc cgcctctatt ttttttattc
      661 gtacaatctc ttttttacag tttataaaag cagtcttctc accctttttt taattgttag
      721 atgccagtgt atgtaaatca ccaatgtgtc atgacacatt ggtgatttta tggtatcctt
      781 ttattattaa ctgacaaagg agagaatatt ttgaacactt tgattgcaaa agacgttttt
      841 agattgcccg aaatgaatgc aagattgttg tcgacctctg ctcttttatt agcccttgat
      901 gtcatcttat acaagatagc gattggtcct gcttattttc aaatcacatt cggattttta
      961 agtatggcgc ttatgggtta tctatatgga ccgatctggg ctggtttgct tgaaatgttg
     1021 tcgaacactt taaatttcac gatatttggt tcaggaacgt ttcaagctgc ttttcttatc
     1081 acggctttcc ttggtggatt tattaatggc ttatttttat atcataaaaa gataaacctg
     1141 ccttccgtca tcgctgccca attattgatt atgatcccag tcagcctttt tatcaattct
     1201 tggctgatgt ctgttttgtt tggtgcagat ttcaaagtaa tttttctagg tagattcatc
     1261 aggaatatga tccaaatccc tattcaaata atggttgttt attcatttat caaaacttta
     1321 gaggctagaa aagtgtttaa acgagctgtt tgattttacg tactaaggta gtaaactgat
     1381 tttggattaa acaataaagc gtgcagtctc aacagactgc acgctttatt gtttctcact
     1441 ggatccattt tatttcatca agtggataat agacaaatct tgctttacct agaatatcag
     1501 ttttattgat cgtgccaaac gaacgactat cttttgaaac tcttcgatta tctccaagta
     1561 caaaataatt atcttccgga agcttggatt gtcccgttag ctcttgtagt gtaaagtccg
     1621 tcgtatagga tgcatttgca tgatctgatt tcaggttttt agttagataa ggttccttaa
     1681 ctactttccc attgatcttc aattgatcat tttggtagct gacattttct cctggaagac
     1741 cgattactcg tttgatataa atcgtgccat ctgctaattg aaaaacaaca atatcaaatc
     1801 gtctgatctc cgaaaatttt tccatcacaa ccatatcacc ttgcttcaac acgttttcca
     1861 ttgaattgcc ttccactggt accgggatga ataaaaatcc ccgcaacatg aacgcaagga
     1921 atacagaaac aagtaagtat ttgaatacaa gccagaagcg attaatatag cgttgttttt
     1981 ttgtcaatct ctatcaatcc atccctcttt ttttcattct ttctttatta taagaaaaat
     2041 atcaaaaaaa tgcgactaga tagctgaaat aaaaataatt ctttcaatta ggctattgga
     2101 caaaaaactt ttttgatcat aaagtacaaa actatctctt atatgtcatg attattttct
     2161 aaatattttt cccatgcttc gtcaaagata tccatggacg gcaaatagtc gacattcagt
     2221 tccagataat tagaaatttc ttcgtagcta gagctgtgct taggaaattg gatgtcttta
     2281 ccggcttctg tagcgaaaac agataaagca tcatgtgtgc caccgcctcg cagtgtcatt
     2341 aaataatgaa aaaagcttcg attcaccctt cgttcctcca attttcttcg ataaaggcag
     2401 cccggcgtgc atggtttctt ttaaaatttt caggatgctt tttataatac tcttgatggt
     2461 cttcttctgc tagataaaat ggttgggctg gttcgatttt tgtaacgatt ggctgttcga
     2521 aacgtccact ttcagctaac tgtttcttac ttttttcagc aatgatccgc tgttcttctg
     2581 tcaaataaaa gatcaccggg cgataattgt caccgcgatc ctcgaattgt ccgaaagcgt
     2641 cagttggatc agtttgctgc cagtaaagtt ctactaattg ttcatatgaa atcaatgttg
     2701 gatcatagat aatctccact gcttctgtat gtccagtcgt ttggcttttt acttgttcat
     2761 aagtcggatc tggtacatgc cctccagtat atcctgacat cactgtatgg ataccaggaa
     2821 gcgtgtcgaa aggctggatc atacaccaga agcagcctcc tgcaaatatt gctcgttcca
     2881 ttatgattcc ccctctaata ctactcatcc gatgattttt tattttcttt tgtcttgctt
     2941 tcagtactgc tggtgctagt tttgttggta tttgagttgt ttgaagcatc agaggtatct
     3001 tttggcaaat atagactaaa ccgaatgtca tcatttacta agtcgatttt atctgcttta
     3061 atatacatcc cgttttgcat tcggaattga tccaacctaa gcatgatagt cagatcatct
     3121 gtattgactt cgatccaatc tggtaattta tagtttctct tgatcatatt catcacatct
     3181 ttgattggca aactcaatgt tccgatagaa agacttctag cttttagttg gacatttccg
     3241 ttttccatca catacggatc aaaatataaa tagaaatcga taggaaaacc tagtacttcg
     3301 aaagtaccat tgagtagtgc ttcattttcc aaataaaatt tatattcaat atcactatct
     3361 ttttgatact cactaaggaa aaagctgatg atttgattga ctttttcttt atttgaattg
     3421 atcgttaaga caggatctcc ttgtctttcc gtaagagctg gtatttctgg gagttctggt
     3481 tcccgattag caaaaatacg gctgcctaaa aatacggtcg aaccaagtac taccccaaga
     3541 aggataagga aggcgaattt ccagtaattt gttttctttg ttttgtttga agatacgtca
     3601 gatcttgttt tcatgtttat tgatcatcct ccttctctaa ccattggttt tgtgtctcga
     3661 tcagctgatc tctcactgca tttgccatca attgataacc taaattattt ggatggaaat
     3721 gatcttcttc atagagcaca ttatttttga tgccatttag attttctgtc gtgctagttg
     3781 tttgatctgt tccttcttct gtgacaccta cttcctgatt caatccttga taaagcaaat
     3841 cattgattgg gataaagtag cagttcggca ttttttttgt caccgtttct gttcctttgt
     3901 tccaattgtt aacaattgtc tgcatgtcag tgatctcagg aaagttcaaa taaaatggat
     3961 tgtagatgcc taaaatgtaa ataggtgcgg ttttattgag ggatcgaaat tcttccaata
     4021 gttctgtgac attttcctga tagtttttca gtggcttttt aaaggaattg accgttaacc
     4081 cgaataaatt attttggata actttcatca aatcattacc gccaacagtc agtgtgataa
     4141 aatcagctgt tttgatattg tttcgaattt cttcttttgt ttttactcgc tttaagatct
     4201 ggtcacttcg ttcaccattt acaccatagt tttctgtttc aaccgcggtc aaattataac
     4261 ggtcttttag atcatttgcg acaaggggaa cgaatcctcc ttgcttggtt tgatctccta
     4321 ctccttcagt cagcgaatct ccgatagcta tgtagtgaag tttttctttt ttatttggat
     4381 cttctctcac agtagttact ggtttcatta gtgggccagc tttcggtata gaagcagcta
     4441 aacctgcata gatgatgatc gcaattaata atgggaacaa aacgaggatc aaatgtcgtt
     4501 tcgttaattt cattttttca cctcttgtat tcgattggtt ctaatgttat caaaaatttt
     4561 atcaaaaagg tagtctttat gaaaaaagaa aacaattatt ttcctaattt tataaagaaa
     4621 aagagaataa gctgcaagcc tttcaggctg agggcttatc ctcttttcga ctaatctaca
     4681 taatacatga ttgcaaacgc gtctttacca gtatgcgtag cgataaccgg attcgtatgc
     4741 agtacaggga tatgcatgtc cgggaataat tcttgcaaac cctctttgaa ttcttgcgcc
     4801 aattcttttg catccgcatg tgaaataccg atttgtcgca catttggcaa ttctttcagt
     4861 tcatttttca aattattaaa ccatttatcg aacgttttct ttcctcgccc cttagttaca
     4921 gggattaatt cagaatgggc gaaattcatc accacgcgta tgttcaaaac actagacagc
     4981 aaaccagttg ttcggctgat tcttccacct tttaccaagt tatctagtgt ggatattccg
     5041 atgtataatt ttgtattgtc ttgtactttt ttgacttctt caaggatttt ctctactgtt
     5101 ttaccttctt ttgctaattc agctgctcga attacctgaa aagataatcc ttggtcagtg
     5161 gtatcactgt cgataaccgt tactttcgtt tttgttagat tactggcttg tcttgctgct
     5221 tctactgttc cactcaagcc ttttgtcata tggatcgaaa tcacttcact gccgtcttct
     5281 cctagctcat catacatcga gatgaattct ccaattggtg gttggcttgt tttgggcaga
     5341 ttttttgctt tcgccatcat ttccataaat ctttcgccgt ctagttggtc atcatcggga
     5401 tatactgttc cgtcgatcat gatggatagc ggcatgacac gaatgcctaa agcatcgcgt
     5461 acattttttt ccatcgtaca tgaagaatct gttacgattt ttacatttgt cattattttc
     5521 tctctctctt tctttaacaa gatgccttat tcatgttaaa ctacatgtaa gacaatttct
     5581 atttttagta taacagacaa ataatgattt ttcattaatt acacctagta aaggagccgt
     5641 tttcatggaa aaaacgaaat ttaatcgaac gtatgaaata ttaaatgaag tgtttaatgc
     5701 agtcacccat ggaattggga ctggattgag tatcgcggga ctagtggttc ttttagtaaa
     5761 aggtgcccac ttgcattcct ccctccatat tgtctcttat gctatctatg gatcgatgat
     5821 gattctttta tttttaacgt ctactttgtt tcattcattg atttttacaa aagcaaaaaa
     5881 ggtgttccaa gtattcgatc atagttcgat ttttcttttg attgcaggaa gttatactcc
     5941 tttttgtttg ctgagtatcc gaggatggct aggttggact ttatttgtat taatctggtt
     6001 gtttgcgatt cttggcatta tatataaagc gttgacgttg cataaacggg agagagtatc
     6061 aaaagtatct acgatcatct atatttttat ggggtggctc tgtgtcgtgg ctgctgttcc
     6121 tctttatcac tctcttggaa tgactggtgt tattttgatg gtagcaggcg ggctttctta
     6181 cacgataggc gcattctttt atagcttgaa ttctatccgc tatatgcatg ttgtttggca
     6241 tttgtttgtt atgttaggtg caggttttat gtacttctct gttttattct ttacttaata
     6301 aaaaaaataa agctatggat gggacagctc taaataacag ctagtctatc catagctttt
     6361 ttatatcgtc aattcgcctg tcccgctttt tacaggattc tcattacgct gatacgtttc
     6421 aaattgatgc ggataacgat ttttttcatc catttctcct tgactagtat tgatcagtga
     6481 ccaatcagac cagtcaacag gtggaaaata agcatctcca tcgaatacat gatggatgac
     6541 tgtacggtat aatatcgtac aaaatggcag aaattcttga taaacagctg accctccagc
     6601 gatgaaaaag ataccgtctg tttctttttc cagagcaagc acgtcttcca cactatgcat
     6661 gacatgcacg ccttctgctt tataggaaga atctctcgtc aaaacaatcg tctgacgatt
     6721 cggcaacgga cggctaccca tcccttcgaa tgtttttcgt cccatcacta gtgtatttgc
     6781 ttctgtcatt tgtttgaaga atttcagatc atttggcaaa cgccaaggaa gacggtcttc
     6841 ttttccaatc aatccgtttt catcctgtgc ccaaattgca gctaacattt tctcatcctt
     6901 tctgattcta aacagcgata ggagctttga tcgccggatg tgggtcatat ccttctaatt
     6961 ggatatcttc catctcaaaa tcaaatactg actgcttttc ctgattcaaa actaaagtcg
     7021 gaaatgcgcg tatttctcgg ctcaattgtt ctttcatttg ttcgatatga ttgatataaa
     7081 gatgagcatc tcctaatgtg tgaacaaatt cgccaaccgc taaacctgtt tcatgtgcaa
     7141 ttaggtgagt cagcaacgca taacttgcaa tgttgaacgg aacgcctaga aacacatccc
     7201 cactgcgttg atacaattgg cagcttagcc gtccctcttg aacataaaat tgaaacatcg
     7261 tatgacaagg aggcaaagcc atgctaggaa catcttccgg attccatgca gaaacgatca
     7321 atcttctaga atcaggagtc tttttgattg cttcgatcac ttgtttgatt tgatcgataa
     7381 agccaccgtc ttttgtttcc cagtgacgcc attgatagcc gtagatgtgt ccaagatcgc
     7441 cgtatgtttc agcaaaggaa tcatcggtta ggatcttctc gcaaaaaagc ttgctctgct
     7501 ttttatattc ttcattgaat tcaggatctg tcaaacatct atggccaaaa tcagtcatat
     7561 ctggtccatt atagtctgga cttttgatgt agcgttcaaa tgcccattca tcccaaatat
     7621 ggtttttatt ttgtaataga tagcggatat tcgtatctcc ttttaaaaac caaagcaatt
     7681 cgcttttgat caatccaaaa ggtactcgtt tcgttgttaa caaaggaaac cctttttgca
     7741 gatcaaaccg catttggtat ccgaataagc ttaatgtgcc tgtgcctgtt ctgtcttctt
     7801 tacgatgccc ttcttccaac aattttcttc ctaagtcaag atacgcttgt tccatccttt
     7861 ttcctcctaa tcttctaccc attcactcaa gtattcccac cgtgccattt tttcttcaag
     7921 ttgtgcttct gtttcagata cgtcgttttg caattcttgt aatcgagtga aattatctcc
     7981 ttgatgattc atctcttctt ggagcaatga gattttttct tcaagttctg caatctcatc
     8041 ttcaatcgtt tcccattctt tttgttccat gtaagataat ttcttctttt cagcaggttc
     8101 cttgctctgt atttttggtt tcgctttttc agcaggctgc tgggtctctt tttgcttcag
     8161 taaataactg gacatgtttc cgtaataaag ttcttgtttt ccgtttcctt ggaatactaa
     8221 taacttatct gctaccttgt ccaagaaata acggtcatgg gaaacagtga tcacagcacc
     8281 tggaaaactt ctaaaataat cttccaaaat cgtcaatgtt gctatgtcca aatcattggt
     8341 cggttcatcc aataataaaa cattaggttg ctgaatcagc agtttcaaca aaaataaccg
     8401 gcgtttttct ccgcctgaaa gtttgcctat cagtgtccca tgcataaatc ttggaaataa
     8461 aaatcgttca agtaattctg ctactccgat ttgactgcca tctgctgttt ttgcttcttc
     8521 agcagcttct tgtaagtaag caatcatccg tttatctggt gccatttctt cattttcttg
     8581 tgtataatac gcaagatgaa ccgtttcacc gatagacatt gtaccgctat ccaggggaat
     8641 tctgccagct agaatattaa gcaatgtcga ttttcccgca ccatttttac cagtaatacc
     8701 tagtctttcc cgagattgaa tcaataaatc taaatgttct agcagtgttt ggtcatttat
     8761 acggtaactg ccatctttga tttccagtac ttttttccct aaacgttgtg tcgctaggtt
     8821 cagctctaaa tcatcttctt ggtttacttg gtacaagttc tcctttagat cttcgaaacg
     8881 gttgatccgt gcttgctgct tggtgccacg tgcctgtacg ccagccctca tccaagctaa
     8941 ttcctgttta taaagccgct tgcgcttttc ttcttgttct acttctacac gatcacgttc
     9001 tgctttttct aagacgtaag cttcataatt tcctttatac tcgtataatt ttccaaatga
     9061 aagttcgaag attcggtttg ctactctatc tagaaaataa cgatcgtggg taaccatcaa
     9121 tagtgcgcca cggtattgtt tcaaatagtt ttccaaccac tcgatcgctt cataatctaa
     9181 atggtttgtt ggttcgtcta gtaatagtaa atcgggttca tcaatcaaca cttgcgccag
     9241 actaactcgt tttttctgac cacctgataa agtactgatt tttttatcta attcactgat
     9301 tcctaatttt tgcaaaataa tctttgcgtt ggtatctgta gtccaagcat cttctttatt
     9361 catttgttct tctgctttag cataacgttt ttgcacatca gggtcttctc cgttttcccc
     9421 tagttcgatc aatgcttcct catatgctcg aacagtttgg atcaaggggc tgtttccttg
     9481 aaagactgct tggagaacag ttaattcatc ggaaaattca gctgcttgag aaagataacc
     9541 aatacggtaa tcagttggat aaaacactgt ttgccgatca ccgtcgctgc tatcgatacc
     9601 ggcaataata tttaagagac ttgttttacc cgtcccgttc gtcccaatca acccgatacg
     9661 gtctttttcg tggaccaaaa aagaaatctg gtcaaacaaa tctttttcac cataagtttt
     9721 tttgaggtca gttactttta attctttcaa catgttccct ccttatcttg gatattgtat
     9781 cataaaaata gtgaacttca ggaaaatttt tttggtattt gttctttttg tgttctcaaa
     9841 aaaagaacag gaccgattcg atcctgttct gatatgtttt gttttttata aaacttgaaa
     9901 atttttacct ttttatttta ttggactcgt tttttttgac tttgctcgaa acgcaaatcg
     9961 gaaagaaaag ttatcagatg gtttgttcat cgtataagat tctaacaaat tgatattcct
    10021 gcgatagctg ttcgcagaaa acgtcgtgat tttttcagca acttcgtatt catcaatgat
    10081 ttttctttca agataataac ctcttagcat ttttttcgta aacagactat cttgctggat
    10141 catccacgaa gaaatcaaat tccccgagtt catttttatt ccttcaagtt tgcgttgctg
    10201 gataaaaaat tgaaccatcg tttcatcata aatatcagtc aagttatcta aactttcaat
    10261 aatcaactga gtattttttt gatatgtctc tctgatttct tggatatctt catcactaat
    10321 aaatgtctga cgatcagcat atcttctttg ccaaaacatt ttcggatgga gaacgattcg
    10381 cattaaccta cggacaacaa tgaaccaaaa gccgataaaa gacaaaatct ggcttgtgat
    10441 agaatgttcg aatcgagaaa gaaaacggga gtaaaaacga taaccattag aagaaagctt
    10501 gcccgaacga taactttcat ccaatccatc ccgttcgatc gacaaaatca gtgcttgtat
    10561 ttcttgtact tcttgctttt catcatcggt taaatcttcc aagtacagat cacgtatccg
    10621 ttcttgataa ttttctatca ctgcttcagt ggctaaaaat tcttttgtat tacgatcaat
    10681 ttcctttatt tcttttcgca aaacttcaac gacctcttct aaaatcatca aagcattcaa
    10741 atccgtgctt ttcgcttcct ctgtttccgt cagcataggc aagacaatga tccctatgac
    10801 caaagtcact aagatgacac ccgccgtcag aaacaaaagc aaagaccgct ggtaaaacat
    10861 catattattg attgaaaatg gcaaaataaa aatcgtagcc aaactgactg ttccctttac
    10921 accgccaaat gttaagatca agatctcgtt caactgtttt ttgaactttt tactgccatc
    10981 tttgaataca taaaaaagac tgatagaaat gaatctgatc acaaaaagca tgacagagat
    11041 taatacgata atcgctaata aaagaccgtt tggatataaa ccgtctcccc atactggcga
    11101 gaacacttgc gtcaattcga ttcctaaaaa tataaatact aatgcattca gtgtaaaagc
    11161 aatagttgtc catgtactgt gggacaagct cgacaattct gcatcaaaca cagagatttt
    11221 cctaaaacca gaagcttgga gaatacctgc tgccactgca gcaatgattc cagatacatc
    11281 aaatgcctct gccaatacat aggctagaaa aggaagcaaa agttcgatca gcagataccc
    11341 tgtgacatct tgtgctgatg ctttttcgat caaatgaatt attttttgtt tgaaccagac
    11401 aagcaaaaag ccgatcaaag ctcccccaat actagaaact actagactca tccccgcgtt
    11461 tactgctgaa aaagatccgg tgatcagcgc accaagagca aactggaagg cagtaacacc
    11521 agatgcatca ttcaaaagac cttctccttc caaaatatgc atcgcctttt tagggatatt
    11581 cactcgtcca gaaagagaac tgactgctac cgcatctgtc ggacctaacg ctgctccaaa
    11641 agcaaagcag gcagcaagtg ggacacttgg caacaggaat gataacgtcg cacctacccc
    11701 gacaagggtc aaaatgactc ctccaaatgc caagaacaaa attgtcccga aatttttgag
    11761 aatcgaagaa atatctgctt tttctccttc tctaaataac aatggtgcaa taatcataac
    11821 taagaacaat tcaggttcaa aatcgattga ttgtccccac tctgtcagac ctaataccac
    11881 tccaaggaaa atttggatca gtggagctgg aatcattggc aaaatgcggc tagctatgtt
    11941 cgataaggtg atcgttactg caaatacaat aactaactca gcaaattcca tcgtccatcc
    12001 cctttatttt ttattttgtg taagcatttt taaaaattgt tcttttttct ttaaaatttt
    12061 ggtttcgact acttcaacct gtgtttggtt cccctctaat tcacactgta cacgttttga
    12121 ctccaaacga agcagatcat gtttcagacg ttccaactga cgatacatca atatatttct
    12181 tttacgcaac ttacaaagta tttgacggcg gtcatccatc gaaagggcgc catagcgatc
    12241 atgcaacagt tttttttgtt gttcaaaact ctctctcatt ttccagatct cctatacaca
    12301 aacttccatc attatagcaa agttcttttt ggatgaaaaa tatttcattg tttcatcgtt
    12361 tattgctagt tagaaatttc cataaatttt tcaacgcctc attctattcc aaatagtatc
    12421 agatatcaag taaaacttaa gaaagataaa agtttctgac aaaaggatgt ttactttccc
    12481 attcctgcct ttcttgttct tttatcacaa caaaaaaatg gggctgcgac aaaaatcttg
    12541 tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt agtaataaga
    12601 cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa aattccccgt
    12661 atacatcttc tacacgttga tctggattga tcacgatata caagttacct gttttttcgc
    12721 tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct agatatggga
    12781 agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg atatcttcgt
    12841 atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata tcatgtgtcg
    12901 caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga tggatttcaa
    12961 caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc caaagatcgc
    13021 cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat tttagcgctg
    13081 tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg tactacctca
    13141 gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa tgacgtatct
    13201 gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt taaatcaatt
    13261 tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt tgttctacga
    13321 cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt tttccagttt
    13381 cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata ggtaaacgat
    13441 cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc atcaattctt
    13501 ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc aaagctccag
    13561 tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt tggttagaac
    13621 acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt tggctgatca
    13681 ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca gcaaaacgta
    13741 aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct gtttcaataa
    13801 atgcatggat tcccgcggcc cgttcatttc ccaagagcat cttgatgaat tccacattta
    13861 tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg tagaaggttt
    13921 cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc attcgtaagg
    13981 catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt tcttctagat
    14041 cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca aacgcattga
    14101 tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg acatgatctg
    14161 gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca tattgttcat
    14221 cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt tttggaaaaa
    14281 gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg attggctggt
    14341 tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct gcattctcta
    14401 attttttcag aactggaatt gccttttggt attccagagg taattgtttt aatttcattt
    14461 ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca tggtgactgt
    14521 ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt tactcgtcct
    14581 tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt tcaaaaactt
    14641 cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa acatcaccaa
    14701 gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct ccataagtgt
    14761 ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc tgagctaatg
    14821 gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc tgcatactgt
    14881 ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa aactttaacg
    14941 caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt aggaaggaat
    15001 ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata tggtcaaacg
    15061 aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg catttggtct
    15121 tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga tcaccttgat
    15181 cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca acatcccatt
    15241 gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg ttctaggtac
    15301 actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa tcaatttaca
    15361 gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag gaagcgggat
    15421 cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga ttttagaaca
    15481 aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg tcttacttgc
    15541 ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt cgtatgtttt
    15601 cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc tacttgtcgt
    15661 ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa gaggtgtgac
    15721 tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct atatggtcgt
    15781 tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg aaaaagcttt
    15841 cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt tgaagccaca
    15901 gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga ggaaagattc
    15961 cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa ttgttatgtt
    16021 cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta acttcatcag
    16081 ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt tgagcataaa
    16141 gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt aattcagtat
    16201 cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag atagatagat
    16261 attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc tttttccaat
    16321 tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc tgcaaaaaca
    16381 atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga tccgcaaaat
    16441 tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca agataattca
    16501 agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac gttaaaaaat
    16561 tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt catctaataa
    16621 tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata attcttgcgc
    16681 tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga aatctggttc
    16741 atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa agtcttctaa
    16801 ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt cttccatctg
    16861 attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat tacttaaagt
    16921 aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa gttcttctgc
    16981 tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa gttcgacttg
    17041 gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa tcatttcttc
    17101 ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat taacttctcg
    17161 aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat tcgtaaaagc
    17221 tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta aaggtacgat
    17281 agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct gcatcgagat
    17341 tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg ctgcctcttt
    17401 gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg gttcatcagg
    17461 aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc cgataacttg
    17521 gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct tatccacttt
    17581 ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt ctgctaacgg
    17641 gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa tctgttttgc
    17701 ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata tatctgctgg
    17761 gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt catctgataa
    17821 agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc tcactgacga
    17881 tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt tggtatccgt
    17941 ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc aaaaaaagaa
    18001 ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga tagccaagca
    18061 agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct tggagattcc
    18121 acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt agatggccat
    18181 tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg ttttctgcct
    18241 ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga gtcgttaatt
    18301 gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca caaatcattc
    18361 cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc tcgctgattg
    18421 tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca cgatttggta
    18481 gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc gatacaggga
    18541 tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc agatccatga
    18601 tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag cgtttgacga
    18661 aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca caaatatacc
    18721 agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac aaatcataat
    18781 agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt agctgactga
    18841 ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa tcaagttcca
    18901 tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc tagaaaagat
    18961 aagagcaaag ctcacctcat aacttgcaaa taaataaaaa ttaaataaaa tacatgtgtt
    19021 ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt ttctgctagg
    19081 aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa ctgtcttatt
    19141 taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta gctggaattt
    19201 cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg cgaacttcaa
    19261 agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct tgaatagttg
    19321 aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta gctgaagcaa
    19381 ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc acaagagata
    19441 tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa aaagaaatat
    19501 tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata gcaaggtttt
    19561 taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac tgtctttttc
    19621 ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag ggataattcg
    19681 gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga aacgttcata
    19741 agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg gtttgacagc
    19801 gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg ttggattgat
    19861 tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa gattctggtt
    19921 catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag tcactgcaga
    19981 aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct cttgttcgaa
    20041 atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga cgataataat
    20101 tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc gaatcccttc
    20161 ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca ttgcacgcat
    20221 cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac cagctgtatc
    20281 tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt
    20341 cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat tgatcaaaga
    20401 ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc
    20461 ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa tccctagtcc
    20521 atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt catagatatc
    20581 gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt tattacttct
    20641 gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac
    20701 acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga tctgatccat
    20761 aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa
    20821 ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt cgacaatcga
    20881 gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg ggcggccaac
    20941 gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa tctcacttag
    21001 tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa gtatttttat
    21061 tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc agctgctttc
    21121 atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct ttttcttcta
    21181 cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta ttttcttctg
    21241 gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt tcttctaaag
    21301 ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact ttcactttca
    21361 cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag
    21421 agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg aagcttgtca
    21481 aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg atttctgacc
    21541 aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca ggattgatag
    21601 acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt tttgctacat
    21661 gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg tcgacaaatg
    21721 cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca tggatagttg
    21781 aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta tgcgataaga
    21841 tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt ttgcctttat
    21901 agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca aaaccacgta
    21961 caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct tcgatgacct
    22021 tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct aaacggcgtt
    22081 ttgaaagtag atagctgccg ttttctttat ctttgccgat cgtactgata acgactaaat
    22141 ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa gttgataatt
    22201 cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc acttgtttgt
    22261 cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct tgtacgcttt
    22321 tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct gtcataacac
    22381 ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt aattgattat
    22441 tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt tttcattttt
    22501 tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta ttgctgcaac
    22561 gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg cttgcttcag
    22621 cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt tcgtcagtgt
    22681 ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt ctgcacgctc
    22741 acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag ctgtcccgat
    22801 gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga cagcaactaa
    22861 ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga catctggttg
    22921 acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat cttcttgttg
    22981 cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat ctgaaaaaga
    23041 tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg tatctgtata
    23101 aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag atgaagcagg
    23161 cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca gaaaaaaagc
    23221 cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa gcataaagtt
    23281 gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg cacgctcagc
    23341 cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg cagctgatga
    23401 agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag ttgcctcact
    23461 tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac ctgtatcttg
    23521 agacacagta gattcttccg cgattgtact agattctact gttgaagtct cagttgactc
    23581 ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag aagaagttgc
    23641 tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg ctggaatagc
    23701 gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc ctttcttttg
    23761 ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg gttgttccca
    23821 tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc tcactgtttt
    23881 acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt cctaaaaaat
    23941 caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg aagttgttca
    24001 gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc aatatcacaa
    24061 caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa ttttctgcgg
    24121 cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt ttctttttcc
    24181 tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat cagctccatc
    24241 catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa ataaagctca
    24301 aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg ttcatcccca
    24361 ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg accgatttct
    24421 tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg gatatcagat
    24481 ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg attttttaca
    24541 aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata gtatccaact
    24601 gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt tgcacaataa
    24661 atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc tttttgctgc
    24721 gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat ctcttttggt
    24781 ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc tgtcgccgtt
    24841 agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg ttgatattct
    24901 ggacggaaat caactcccca ttgagacacg caatgagcct catctactac atataacgca
    24961 atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc gggactcaaa
    25021 aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga ttgcgaaagt
    25081 aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt cacttgatct
    25141 tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa ataccccgtc
    25201 aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt atcttctcca
    25261 tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa tccaaaatat
    25321 ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat agatttgata
    25381 caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa agcgccactc
    25441 tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga gaatccactc
    25501 gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta ctgttcctct
    25561 ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa acatctgctt
    25621 ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca aagcgtgcca
    25681 aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc tgctttgttg
    25741 cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca gccctgttcg
    25801 ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta tctccgaaaa
    25861 cagctgcacc aaactctcat acgcagtatc aattaaaatt tcacgtggca attggctttc
    25921 actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg gaagatagtc
    25981 ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga gccgccaact
    26041 agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta tttgatgttc
    26101 ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta tttcgatcca
    26161 accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca attctggaaa
    26221 actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg aagtcgttcg
    26281 ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg atgaaaatag
    26341 cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga aatgtcacta
    26401 ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat cgaagcgcca
    26461 gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc agaggctcat
    26521 caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca aaaatgtcca
    26581 gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta aaaacgtaca
    26641 ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg tgaaatcccg
    26701 acttattttg aaagattcat ttgctatcta tagttaattg actagttttt tcacggaccc
    26761 tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt attgacgatc
    26821 acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg ctcaccatag
    26881 ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct ttccggtaaa
    26941 tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt cgccgtgatc
    27001 gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata acttgccatg
    27061 aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg acttgccatt
    27121 acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc ttctttgacc
    27181 ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac aatagcgact
    27241 gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg ctctacgaga
    27301 gcaccaggat aaagaataag taatggagac tttccatgat tctttttctc tccttcaaaa
    27361 aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc tgacgatggt
    27421 tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc taaaatgatg
    27481 actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt ctatctgagt
    27541 gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag aaatgcaaac
    27601 tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc tttttgtatg
    27661 tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc attgctttat
    27721 acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga gtatcaggga
    27781 acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg ccgcctgata
    27841 caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga tttttcacta
    27901 actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca taagtcaacc
    27961 catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg aatgtaacag
    28021 ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa cttgcaaagt
    28081 tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct tcaccagttg
    28141 cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat acttctgaaa
    28201 gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc gcgtggatac
    28261 ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg tgttttttca
    28321 atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca ggaacaacat
    28381 tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc aagtaagctt
    28441 gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca aatccttgcg
    28501 taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt tcttcatctg
    28561 taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc aaagctttga
    28621 cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga ccaactaacc
    28681 agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg tcccctgctg
    28741 gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca atctctgaat
    28801 aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca ctgatttcca
    28861 atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt cctgaatctg
    28921 cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat gcttgttcat
    28981 gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt aaaaagggtc
    29041 atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta agcctgctcc
    29101 atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact gaatggagct
    29161 attgacttat ccacgtgctt atcgatagcc gtttatcggt ttcttcattc ttatgataga
    29221 ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga tcacacagat
    29281 ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg aaacatcgaa
    29341 acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta agtttactaa
    29401 accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg caaattcacc
    29461 cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag gaatcaagaa
    29521 tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag ataatgaact
    29581 taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt tcataaccac
    29641 ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag aagcaccagc
    29701 taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta ctacgatgga
    29761 cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg gaagcatgct
    29821 tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca atgattcaaa
    29881 aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca tgataccaaa
    29941 agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt cttgacgacc
    30001 gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata ctaatgattt
    30061 tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag aaatagctaa
    30121 caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa tcccaacagc
    30181 ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc ctacttgaga
    30241 ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca tcatgactgg
    30301 gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag agccgcctaa
    30361 tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga atttcttcac
    30421 aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta aaaatccacc
    30481 aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag ttagctcccc
    30541 cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca gcccttgagg
    30601 attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt ctacactata
    30661 gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa taaataagac
    30721 cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct tcttcttcct
    30781 ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa aagagtatca
    30841 gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg tttttcccta
    30901 ttccctcctc ttttttcagg tt
//
LOCUS       DAZUMV010000020        29394 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000020 DAZUMV010000000
VERSION     DAZUMV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.020"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..2184)
                     /gene="copB"
                     /locus_tag="ACG8FK_000840"
     CDS             complement(13..2184)
                     /gene="copB"
                     /locus_tag="ACG8FK_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS5096469.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2204..4390)
                     /locus_tag="ACG8FK_000841"
     CDS             complement(2204..4390)
                     /locus_tag="ACG8FK_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5096470.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="ACG8FK_000842"
     CDS             complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="ACG8FK_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS5096471.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4612..5052)
                     /locus_tag="ACG8FK_000843"
     CDS             complement(4612..5052)
                     /locus_tag="ACG8FK_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS5096472.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5126..5665)
                     /locus_tag="ACG8FK_000844"
     CDS             complement(5126..5665)
                     /locus_tag="ACG8FK_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFS5096473.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6280..7746)
                     /locus_tag="ACG8FK_000845"
     CDS             complement(6280..7746)
                     /locus_tag="ACG8FK_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5096474.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8057..10138)
                     /locus_tag="ACG8FK_000846"
     CDS             complement(8057..10138)
                     /locus_tag="ACG8FK_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5096475.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10662..11021)
                     /locus_tag="ACG8FK_000847"
     CDS             complement(10662..11021)
                     /locus_tag="ACG8FK_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS5096476.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11051..11392)
                     /locus_tag="ACG8FK_000848"
     CDS             complement(11051..11392)
                     /locus_tag="ACG8FK_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096477.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11389..12063)
                     /locus_tag="ACG8FK_000849"
     CDS             complement(11389..12063)
                     /locus_tag="ACG8FK_000849"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5096478.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13240..14538)
                     /gene="asnS"
                     /locus_tag="ACG8FK_000850"
     CDS             complement(13240..14538)
                     /gene="asnS"
                     /locus_tag="ACG8FK_000850"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS5096479.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14578..15768)
                     /locus_tag="ACG8FK_000851"
     CDS             complement(14578..15768)
                     /locus_tag="ACG8FK_000851"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5096480.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15789..16316)
                     /locus_tag="ACG8FK_000852"
     CDS             complement(15789..16316)
                     /locus_tag="ACG8FK_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS5096481.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16363..19152)
                     /locus_tag="ACG8FK_000853"
     CDS             complement(16363..19152)
                     /locus_tag="ACG8FK_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS5096482.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19299..19499)
                     /locus_tag="ACG8FK_000854"
     CDS             complement(19299..19499)
                     /locus_tag="ACG8FK_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5096483.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19951..20271)
                     /locus_tag="ACG8FK_000855"
     CDS             complement(19951..20271)
                     /locus_tag="ACG8FK_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096484.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(20655..21773)
                     /locus_tag="ACG8FK_000856"
     CDS             complement(20655..21773)
                     /locus_tag="ACG8FK_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096485.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            22006..22128
                     /locus_tag="ACG8FK_000857"
     CDS             22006..22128
                     /locus_tag="ACG8FK_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096486.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            22244..23551
                     /locus_tag="ACG8FK_000858"
     CDS             22244..23551
                     /locus_tag="ACG8FK_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS5096487.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            23670..24869
                     /locus_tag="ACG8FK_000859"
     CDS             23670..24869
                     /locus_tag="ACG8FK_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5096488.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(24896..26164)
                     /locus_tag="ACG8FK_000860"
     CDS             complement(24896..26164)
                     /locus_tag="ACG8FK_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS5096489.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(26219..26285)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(26327..26974)
                     /locus_tag="ACG8FK_000861"
     CDS             complement(26327..26974)
                     /locus_tag="ACG8FK_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS5096490.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(27382..27885)
                     /locus_tag="ACG8FK_000862"
     CDS             complement(27382..27885)
                     /locus_tag="ACG8FK_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5096491.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28008..28772)
                     /locus_tag="ACG8FK_000863"
     CDS             complement(28008..28772)
                     /locus_tag="ACG8FK_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS5096492.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            28880..29155
                     /locus_tag="ACG8FK_000864"
     CDS             28880..29155
                     /locus_tag="ACG8FK_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS5096493.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         8284 a         6313 c         5157 g         9640 t
ORIGIN      
        1 cttttttcat tattatttga ttttcaatgt caaagcattt agagctacaa ctaccgtact
       61 taacgacatc aagacagcac caactgctgg acttaatatg ataccaattg gcgcaagaat
      121 tccggctgct agcggaattg caagaatatt gtaacctgcc ccccaccaga ggttctggat
      181 cattttcttt cgcgtttgct tggctagatc aagaaaatgc aaaatatctt ttggatcgct
      241 gtttgtcaat acgacatccg cagaatcgat tgcgatatct gtccctgcac cgattgccat
      301 cccgattgaa gcacgggcta aacttggcgc atcattgatc ccatcaccca ccatgattac
      361 ttttttccct tggtcaaggt atttctggac aacagcttct ttatcatctg gtaataaacc
      421 gccataatat tcttcgatcc ctaaataatc tgctactgct tgggcagcgt ttttattgtc
      481 accagtaagc atgacaggag tgatttttct ttctttcaat gttcggataa attcttttgc
      541 ttctgttttt actacgtcac ctaaagcaac gattgcagct agatgatttt ctaaaatcag
      601 gaagctgatg gtatttcctt gttcttggta aggtttcaat aatgcttgtt ctacgtttaa
      661 tccgagacgc tcaacttctt tttcattaac tattttcact tgttgttttt tgactgttgc
      721 ctctagcccg actcctgaaa gattttttag attttcagct tgataaggct tgatttcatg
      781 ttctttcaaa taattcataa tgccaacagc caatggatga ttggcattcg cttccaaggc
      841 tcctagatat tgaaggattt catttttatt gaacgcatca tcaagtactt cgacacccgt
      901 tacagtaaat tttccttctg ttagtgttcc tgttttgtcc agcatgatca catctagatc
      961 attggcttgc tccagtgcat tgcggttttt caaaagtaat ccgtttttag ctgctattga
     1021 tgtggagcga gcaacaacta atgggatagc taaacctaga gcatgaggac aggcgatgat
     1081 aaagactgtt accatacgtt ctaatgcatt aggaagatct gtcaagaaca accaggcgat
     1141 gaatgccagg atacccacaa ttaatgcgac ataaaacagc cattttgcca ctttgtcaga
     1201 taatgactct agcttggatt tttctccttg tgcttttcgc accatttcca taaccttcgc
     1261 tagataacca ttttcaccag ttccagttac ttcgatttcg atcgttccat ccccattgat
     1321 agaaccgcca atcactttgt cattggcttg ttttttgatt cctcttgatt cgccagtcac
     1381 agctgattcg tcaacaattg tttcaccttt caagattaca ccatctgttg gcattttatc
     1441 accagaccgg acgatcaaac gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc
     1501 ttttccatct ttcgtcaagc gtttcactga ttctggcagt aattctgcta gtttttgtaa
     1561 agcattgctg gcattcgaca cagcattcat ttcaatccag tgacctagta gcataataac
     1621 gatcaaagta gccaattccc agaagaaatc catgacgtgt gtatgtggat tgagcagatt
     1681 tgcgataaac gaatagacac tataaatata tgcaactgta atccccatcg cgatcagtgt
     1741 catcatagca ggactttttt gtttcaattc catcttagct ccacttaaaa atggctgacc
     1801 gccatagata aatagtatag ttgctaagat caatacgagc caatcagaac caggaaacgt
     1861 tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa agaatgattg ggatagctag
     1921 gataagcgat agccaaaatt tttgtttgaa attccccatg tgcatggaat gatccatgcc
     1981 gccgtgttcg tgatccatgt gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc
     2041 atggttatgt cccatgtgca tggaatgatc catctgacta tggtcatgtt tcatctccat
     2101 ctgtttttca tgagattctg tactttcttt gtttactact tcttttttat gctcatgttt
     2161 tttctccata tcgttcccat tcatttttac tccatccctt ctcttacttt ttcatgtgtc
     2221 gatttaagct cagggagttc aacaatacac tgacggaact aaacgccatc gctcctcctg
     2281 caatgattgg attcaaaaag cctagtgcgg caaaaggaat accaatcgta ttatagacaa
     2341 aagcccagaa aagattttgt ttgatttttc tcaatgtcaa cctagacaac tcgattgttt
     2401 gtgcaatgga tgccaaactg ctattcatca gcgtcacatc ggctgtttcc atcgcaatgt
     2461 ccgttccact tcccattgcc ataccgacat cagctaatgc taaggccggt gcatcattga
     2521 ttccatcgcc tgccataccg acttttttgc catctttctg aagtttttct acgtaggctg
     2581 ctttatcctc tggaagtact tcagcaaaga tatgctttgg atcaatacca acttgtttac
     2641 caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat aaaaacatct aatcctttat
     2701 tctggagctg cttgattgct tgtttggctt ctagtttgat ttgatcagcc acggcaatcc
     2761 acccgatcac ttgcttttca tcagctaaaa acatgactgt ttttccctgc tgttctaatt
     2821 ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg
     2881 tacccgcaaa atagcgtact ccattaatcg taccgctgat cccagcacct gggtgggctg
     2941 taaaatcaat catatcgtat gttgctgctt cttgcagttt tccgtattcc acgattgctt
     3001 tacctagtgg atgttcagaa gcttgttcta aagtataaaa aatagatagg acttcttttg
     3061 aaccgactag atctgtaact ttgggctttc cttctgtaat cgttccagtt ttatccaaaa
     3121 caatgctgtc taagtgagca gctgcttcta atgcttctcc gcctttaatt aaaattccat
     3181 ttcgtgcacc aacacccgtt cctaccataa tagctgttgg ggtcgctaaa cctaatgcac
     3241 atggacacgc aatgactaat acagagacac tatggatcaa tgcgagttgc caatcaccag
     3301 tgatcaaacc tgtagctatg agcgtgataa acgcgatgac taatacgatc ggaacaaaaa
     3361 tccctgagat ccgatctgcg attttttgga ttggggcttt gctcccttga gcatcttcca
     3421 ccatttgaat gatttgcgct aatactgtct gcttgccgac ttgtgtgact tccatgtgta
     3481 gcagaccatt cgtattgatt gtcccgccaa aaagcgtgtc atccggattc ttttctacag
     3541 gaagattttc accagtaagc atactttcat ctatcgcact gcttcctgaa ataattcgac
     3601 catctgctgg aatctgctca ccaggacgga tgactagttg atcaccaacg actacttctt
     3661 caatcgctaa cgtttcttct ttcccgtttc ttatcacttg ggcggttttt gtttgaagag
     3721 acatcaattg tttgattgca ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc
     3781 cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa ataaagatct tgggggtgtc
     3841 cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc cgatgttcca attgctacca
     3901 atacatccat atttggtgct ttcgtcttga ttgcatggta cgcaccttta taaaaacgag
     3961 caccgacata aaattggaca ggagcagaca gcactaactg gacgattgga aaatgaaaaa
     4021 agtgaacaat cgccgcatgg cttcctaaca tcatagcaat cattgacaac atcaacggca
     4081 aagtcaaaac cgtactcaat ataagatcga atagcatttt tttcaaatac gcttgctttt
     4141 cttctgcaat tttttgtttg tgggcttcat cgtacaaaat tgcaccataa ccgatgtttt
     4201 ctacgctttg gattaatttt tcagtggtcg tcccttcata tttgacactt gctttttcag
     4261 tggccaagtt gactgtggca ttgaccacac caggttgtcc gttcagctct ttttcgatcc
     4321 tagcagaaca atttgcacaa gtcatacctg taatgacgaa cgtatccatt ttggtttctg
     4381 ctgttgccat caaatcacct ctgctttata acccaatcca ttgattgctt gacaaatctt
     4441 ttcggattga acttttgttt catcaaactt gaccgtaccg tttgcttttt tcaaatttac
     4501 tttcactttt tgcacgccgt caagtgcgct tactgtttct tctactcttg ctgcacaatg
     4561 attgcagctc ataccttcga ttgaaaattg ttgtttcatt ttttcttcct cctattgatg
     4621 ttgtttacat tcacattgac ctgggataca gttacattcg atcgtatcta ctggttgctt
     4681 ttgttccagt tgtttcaaaa tctcattgat atcttcttga gtcaaagtgg cttcttctac
     4741 tagatccgca atcgtctctc caatcctttt tgcacagata tgggaaaaaa gattttccgt
     4801 cgcactgcga actgtttcca tctccgagac agctggatga taaatgaatt tttttccttc
     4861 ttgttctgtc cagatcactt ctttttttac caaacgtccc agcaaagttt tgatcgttgc
     4921 tggtttccaa tccatcgaat cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc
     4981 aagtgtccaa ataacacgca tgatctccca ctctgaatca ctgatcttta ctggcgtttt
     5041 gactttttcc atttatttca cacctttcgt ttacaaatgt aatcattagc ttatgaatac
     5101 agattacgct tgtaaacaaa aactgtcaat tatttttgct ctctattttc aatttagtta
     5161 aaaattcttg tttctctcgt cttgttttaa ggatgatcag tttatttctt gcattagcta
     5221 cttggatcag ctgctgtatt ttcggttcat tgttttcttc aaaatgccag ataaaagata
     5281 aaaattctaa gtattccctg tttagtttct ctgaaaatga ttcaggcata tctggccgag
     5341 attttttgtt tatctttctt tttagagaac gccaaactac tcggtagatg gcgagataac
     5401 gcggcacttt aagccaaatg atcttatctg cttctttaat cctttcctct aatgtagaag
     5461 tataatttcc ttcaacaatc catgacggat tagataccat aaacgattgc tgttcttgca
     5521 aaaaccactt ttttgcctga atactgtagt ctgttgtatg ccataaaagg tctagcggca
     5581 gtagcggaaa gtcggtgatt tctttgatct tccttgcgag ggtcgatttt cccgacccac
     5641 tttgtccaat cacgatgatc ttcattcttc tccctcactt ctctttggaa tttggttcga
     5701 tcataccact tataacgccg caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc
     5761 ccttgtttcg aataaatggt gttcaatcag ctgaccttca gcattaactc aaaaaacggc
     5821 aaaatatgag gagctatttt ttcgtttgtt cctctaatac tcaggtcaaa tcgctgattt
     5881 tacaacctct tccttaaacg gtgttctcct gccaacacct cggtcacaag gcacaatagt
     5941 caacaaacat gagaagttgt tttttgaaat tgctccttgt gactcggagt taaacggcag
     6001 gctcactacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagcccagc
     6061 aaaacgaaaa atatgagggg ctatttttgt ttggctgttc ttattacttg atgcaggaca
     6121 gctttttcac aacctctgat tcacggtgtt ccagaagtag cttctgcgaa aaacaagccg
     6181 aaacttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
     6241 ccacttctgt cacaacctct tgtaacaagt atttctattt taaggctgaa ccactgtttc
     6301 tggttcagtg atttctttgt ttttgtctcg acgagcaatt gctggcaaga tcatacctaa
     6361 taagatcaag acaattggtg tgatgatatt cgtgaataat tggaaccacc atgctttagg
     6421 atcagcagta taatctactt ttggcaccat tcctaggata catgcaaacg cagtgaaggc
     6481 aaagcaccaa gcaccaaaga taaaaccgat ttttggattt ttaacaaatt tatattcaga
     6541 attgaatttc ttataagctt tattcaacat catatatgcc aagaacaccc ataggtaacg
     6601 cattggcatg acaactgagt tcaagttcgt caaccatttt actaattcat tcatatcttt
     6661 gataccaaag atcggcaaca aaataatgat tcctactagt atacctgtca gaagataacc
     6721 attcaccaat gtacctttac gattacgttt acgtagccag tttggtacaa attctggatc
     6781 cgcatctgct aacaagattt ttagtggtgc atcaattgaa aaggctaatg cggagatttg
     6841 tccaatcatg tttgtcaatg cgtaaatgat catcaatgag ttaccgatac catagtaatt
     6901 acctaaaatt tgaaaagcac tgtatgcccc atttgccatc aagtctttag gaatattatc
     6961 gctagcaaac agcataccca ttgcgactga gcctaataca gcacaaatcc ctaccattgc
     7021 tgctaagaag atcatccctt taggaaattc ctttgcagga tttcttgtag agttgacata
     7081 aggggatatt ttttccgctc ctccaactgc aaatactagc atcgaaacgg ttgtgaaata
     7141 actgaaatca aatgttggaa tatatgtttt cacttgattc atatgtggtg tggcaaattc
     7201 catcgttgga ttgatgaacg gtgccgttac agctaaaaga ataaataaga tcgacatgac
     7261 gaacatcgct gttccggcca gtccgccaat cactttcaaa gtagaaagtc cttttgttga
     7321 taaatataaa aaggcaagaa atataataag tgaaataact gctaccattt gtactgacat
     7381 cgtatttacg atatttccat ttccttggac tgcccaaccg ccagcaatca ggattgcttg
     7441 aggcttttgt gccaaataag gaatatggac gacccagtaa gtccaagcgg catagtatgc
     7501 cagtcgttta ttagatgtcg tattttcgac ccatgaactg acgcctcctt tactatcctt
     7561 gaaggctgac cctaattgtc caacgatcaa tgcgtatgga ataaaataaa tggccaaaat
     7621 caatagccaa gaggtgacaa ctgatactcc ttgctgagca tagttattaa ctacattccc
     7681 catgccccaa accatgttaa atgcaattaa cgcaacagta aaccagcgta attgtttgtt
     7741 gttcattcgt tctctccttt atgataaaaa tttgttatcc agatgtaatc atattcaatt
     7801 tttaacaaac ttacaagaca gtgttatttt atgtgtgtat tcttttattt aatacacatc
     7861 gttttttcat attttttcca tcaaaaaaat aagttatttt cttttatttc atttacctta
     7921 ttgaatttca agctattttg aattttctgc aaaattaaaa acattagtta ttttataatc
     7981 ctatctattt tctttacatt tttcattaag aaaaggatat aaaaaaaaga ggacgttcat
     8041 tcgtcctctt cattgatcaa cgtttatttt ttacatgcaa gcttcgcatg gcattcaaaa
     8101 cagctaatac tgtcacccca acatctgcaa atactgcatc tcccattgat gcaaaaccaa
     8161 aagcacctaa ggcaagtacc aaaattttta tcccaatagc aaagacaatg ttctgtttga
     8221 cgattttcaa tgttttcttt gctaaatgga ttgcttctgc aatcttcgat ggttgatcat
     8281 tcatgatgac tacatctgct gcttcaattg cagcatcgct tcccaatcca cccattgcga
     8341 ttcctacatc tgctcttgct agcaccggtg catcgttgat tccatctccg acaaatgcgg
     8401 tctttttatt gtttctttgt aagatttcct ctaaccgctg cactttatct tctggaagca
     8461 attcgctata tatcttgtcc acgcctactt gcttcccgat atgatcagca atttttttcg
     8521 aatcgcccgt caacatgact gtgttcttca caccagcctt ttttaattga accaatgcgt
     8581 ctacggcatc ttctttcaag gtatctgcaa taacaagata accactgtaa ctttgatcga
     8641 tcgctagata aagcaatgta ccgatttctt gacttgaagg aaaagagatt tcaaatcttt
     8701 ccatcaattt gtgattacct gctaatactt gtttgccatc aacttcagct aagatccctt
     8761 cacctgcaaa ttcttggata ttagctgttg gtaaaagcgg acctgcgtag ccttctaaaa
     8821 cagattgagc aatcggatgt gtagaaaact gttctgcact cgcaacatat tgcatgaata
     8881 tctttggatc gatttttgta tcgatcgtct ggatcgaaaa gtcacctttc gttaatgtgc
     8941 cggttttatc gaataccact gtctcagttt gtgcaagcaa ctccaagtaa tttgaacctt
     9001 ttactaatac accgatttta cttgctcctc cgataccgcc aaagaaacta aggggtacgg
     9061 agatcactaa tgcacatgga catgagatca ctaaaaatgt caatgctcga tagatccatt
     9121 cagaaaacgc cgctccagaa acaacaagtg gaggtaaaac agctaataaa acggccaatc
     9181 ctacaacgac tggtgtgtaa tagcgcgcga atttagtaat gaaattttca gctggtgctt
     9241 ttttactgct ggcattttct actaattcta ataatttgct gatagtagaa ttttcataag
     9301 atttttctac ttgaactaaa aatggtttat ttttattgat aaatccactt aatactgttt
     9361 caccaacata tacagattta gggactgatt ctcctgtaag tgctgacgta tcgactaatg
     9421 attctccttc gatgatcgtt ccatctaacg gtacacgttc accaggcttc acaagtacaa
     9481 actgaccgat ctgcacgtct tcagggttca cggtcatcaa tccttccgtc gtctgcacgt
     9541 gagctgtttc tggacggatc gccatcaatt cgcgaatcga ttttcttgaa tgactgactg
     9601 ccaatccttg gaaatactcg ccgatttgat aaaatagcat cactgctacg gcttctggat
     9661 attcaccgat caacatagcg ccaatcgtag caacagacat aagaaaattc tcatcaaaga
     9721 tttttccttt gacgatattc atcactgcct tcttcactac atcatatcct atcagcagat
     9781 aaatcaataa ataccctatc aagcgaattg gttcattaaa atcaaataat agcaataaag
     9841 cgatacctgc aagggaaaat aaaatccgga acagcgtatt tctctctttt tcatttccat
     9901 gttcagatac tttttcctta tctatttctg ttactttgac atctggttct aattccttca
     9961 ctactttttt tatttcttgt tcaatcgtag ccaaatgatc ttcctctgcg tcaatcgtta
    10021 gttttccaga agtgaagttt accgttgctt ccttgactcc attgatcttc tgaactcctt
    10081 tttcgatttt cattgcacag ttcgcacaat ctaaaccttc taatctgtaa cttttcattt
    10141 taatgacctc ctcgcctcaa catatgaata tctcttcata actaattata aatgaacggt
    10201 tattcatatg tcaaggtatc cgaaagaaaa aatgaaaaac atctgcatcg gaaaatttct
    10261 tccatacaga tgttttggct tattctttct ttttcttttc gatcattgcc actgctctta
    10321 tccaacccgc acttgccttc ttgatcttga caggatagca actaagtttg aagcctgttg
    10381 ctggcaattt gtctaaattc attaattttt cgataggaca atatcttttt tcaatccctg
    10441 caaaatgtcc ttcccagata ttcgaagctc cactacgctt tcaaaaatat agaaagagtt
    10501 ctttaatgaa aattatttca actttccata aacagtaacc ccatataaat aaaagacgta
    10561 aaagtcacat ggaagaactt atacaggaga acgcatagag tcaggtgaaa tacatgtatc
    10621 ccacctgact ctatgcttta tctaaccttt attattctct tttatacaat ttctgcaccg
    10681 aatggcatca acgccaatcg agcaagtttg aaagattgct tggcaaaagg tatccctatg
    10741 atcgtgatac atagaaggat gccgctgacg acatgtgcga gagcaattgg taggcctgaa
    10801 acgatcagcc agatgatatt aacaatcaat gacatgccag actggtgata aattacttct
    10861 ttgccaaaag gcgcaaaaga aagggaggcg atcttaaaac attgcatacc gattgggata
    10921 cctatgatcg tgatacacca aagtatcccc gctgctaacc agctaagacc accgattagt
    10981 ccgccgaata taaaccagat gacatttcct aaacaactca ttgtatttct ccttctatta
    11041 ctgtttttga ttaaaactgg gcaaaaaatg attttattct tcttttttta gcttccagta
    11101 tcaaatattc tgcaaatttt ttttggattt cttccgtatt ttcttttgtt actaatgtgt
    11161 gttggattag ttttcctgat ggtcgctgat ccaatcgttc ttctgactgt tgcacgtccg
    11221 cttgcgcaaa tccgataatc cgtttttctt ttgttacagc aacgagcaat agtttattct
    11281 tatcatttag gtcagctgcc gtttttgctt ttacgaatga ttctggatga tcagtaatca
    11341 gctgattgat ttgaggtaga tcttctttta ctgcccaccg tatttccatc atttctcact
    11401 tttctttcgc tcatttttat ctccactaaa aaaatcgact gcggcaattg catctatcca
    11461 gctttgtgca atgatctcat gtccgccaat cgttgggtgg accccatctt ctcttgtata
    11521 tttcgaaaaa ccataaacaa tccctaaagc attgaatata ccatctaaag gaaccagcaa
    11581 tgcgccatat tccttagcaa gtttacgaac aatttggatt ttagggtcta agtctttacg
    11641 ccagccatga cggtcgtcgg ttataggaag tacaaacggc tccatcaaga tgatgttgca
    11701 gattcccact gtttgaagtt catttagtaa ttttcgataa acggattcaa attgctgagc
    11761 agatttctca gttccgaata tagcctgatc gctagtatta tgccaggtat cattgattcc
    11821 gatcaaaatc gatacatagt caggttgtaa agctaaacaa tcttcttgcc agcgtacttg
    11881 cagatctgca attttgtctc ctcctatccc acggttcatg cattcaaatt ccatcgttgg
    11941 atattgggag attagttttc cggcaatcaa aagtggataa cctttaccta gatcagttgc
    12001 ttctgctcgg tctcttcctg cgtctgtaat gctatcaccg ataaataata tacgtttttt
    12061 catttgtcaa aaactccttt ttgaataaaa cgatgatttt cttccttata ttatcccata
    12121 agccctatca agacgaaaga gaaaatatat gtataaaaaa tgacaccagc ctagtccctt
    12181 caaacaattg aatgaacgct catcaagtgt ctagtttttt atgctatttt tttcttaacg
    12241 tgccggattc cccttgattc cttcgttgtt tggtccagag agatcgatca cacctaagct
    12301 tccgtcaatc agaccactga tgacgccatc agaacatata tttataggct catctaagac
    12361 ttctagtaaa tgtcccttta ctaatatctc ttctagattg cttgggatag attggtcata
    12421 actttatctg gtccgatgat ttcaagggtg aattgaactt acatatggat tattgcatgc
    12481 tgaaaatacg gacctactcc atcttcgaat gttactatta gtttgttttc aggtgctaga
    12541 tgctgcttta tttcaacagc agaagcatca tcaaatcaga tttccatttg aattaccttc
    12601 tttgtaaatg tttgattgat tgcgatttcg ttatagaacc ttgtgaatca aatagcaatt
    12661 tcttttgctt tcttctcttg tctgctggaa acttccttta tcccgtattt taaaaaaaga
    12721 actgcgagag ttcgcagttc ttttttagaa taaacggtgc tctccggcca actcctcggt
    12781 cacaagtcac aatagtcatc aaacatgaaa aactgttttt tgaaattact ccttgtgact
    12841 cggagctgaa cggcaggcgc acaacctctt gatataaacg gtgttcaatc agctgaccct
    12901 cgatattagc taaaaaaacg gtaaaatatg agaagctatt tttccgtttt gtttctctaa
    12961 tactcaggtc aaggcactga tttcacaacc tcttccttaa acggtgttca aaagccagtt
    13021 tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc catttccaca aaatttttct
    13081 taatactcga aactaaacag ctttttcaca acctctccac taaacggtat tcaaaagctt
    13141 acatctgcgg taataagtcc cgccaattca aaaacatgaa cagctgtttt tgcttggctg
    13201 ttctaattac ttgatgcaga acagcttttt catcacctct tatggataaa ttcggttcaa
    13261 caaacgtggg aatggaatag cttcacgcac atggtcgata ccagcaatcc aagtgactgt
    13321 tcgttcaagg cctagaccaa aaccagagtg cggaacagaa ccgtatttac gtagatcaag
    13381 ataccatgaa tattcttttt catccaaacc gtgattacgg atttgttcta gcaagaaatc
    13441 atagtccgtt gcccgttcgc ttccgccaat aatttcaccg taaccttctg gtgcgatcat
    13501 atctgcacaa attaccacgt cttctcttgt tggatgtggt ttcatgtaga atggcttgat
    13561 agcttttgga tagtttagga taaagacagg ctgttcgaat gaattcgcaa tgaacgtttc
    13621 atgaggtgaa ccaaagtctt cgccccattc gatgtcatca aatccatttt tcttcagtaa
    13681 ttcgatcgca tcatcataag taatacgagg gaatggcaat tttgtataag tttttaagac
    13741 ttctttgtct cgtcctagta catctaatgc gtattcgcaa tgatctaaaa cattttgtac
    13801 taagaatgct acatattgtt cctgtacttc taaactttct tcttgatgag tgaaagccat
    13861 ttctggttcg atcatccaga attcaatcaa atgacgtcgt gtttttgatt tttcagcacg
    13921 gaaagtcgga ccgaatgaaa agacttttcc gaaagccata gcagcagctt ccatgtataa
    13981 ttggccactt tgagataaat aagctttttg atcaaagtaa tttgtttcga atagatccgt
    14041 agtgccttct ggtgcggaac cagtcaagat cggcggatcg attttgacaa aatgatgttg
    14101 attaaagaat tcatacgttg cacgaatgat ttcatttctg atctgcataa tcgcatgttg
    14161 tcttgaagaa cgtagccata aatgacgatg atccatcaag aagtctgttc cgtgttcttt
    14221 tggtgtgatc ggataatcgt ggctctcgct taccacttcc acatttgtaa ttccgatttc
    14281 ataaccaaat tttgaacggc tgtcttcacg gatttctcca gttactaaca cggatgtttc
    14341 ttggtgcaga tttttagctg tttggaaaac ttcttccggc acttcgcttt ttacaactac
    14401 accttggaaa aaggctgttc cgtctcttaa ttgtaaaaat gcgatttttc cgcttgatcg
    14461 cttatttgcg acccaagcgc cgattgttac tgtttctccc acatggtttt tcgaatcgat
    14521 gatttgaatt tgttccacgt atgactctcc ttttatattc cttgaatttc ttcaggatta
    14581 attttgactt ttactctcaa taaattgctt gattctgcgt accgcttctt ctaacgtagc
    14641 gcgatcagtg gcataactca tgcggacatt ttctggtgcc ccaaagcctt cgccagtcac
    14701 aagcgctacc cctgtttctt ctaaaagatc ttctacccat tgtgtgacat tttcatattt
    14761 acacatgcgt agcgtttctt ttacattagg aaaaagataa aaagctcctt gtggcttttc
    14821 aagagaaaca cccggcagtt ctgccacaag cggatataac gtattcaggc gttcttcaaa
    14881 ggcgttccgc atttcttcca ctgtatcttg ctcgcctttc aatgcttcaa cagctgcata
    14941 ttgactgact gctgtcggat tgctagttga ttgggaagca acagcgatca ttccattgat
    15001 gatcacagga tttcccaccg cataaccaat gcgccagcct gtcatcgcat atgttttaga
    15061 cacgccgttg atgatgatgg tctgattttt gattgcttct gaaatcgttg caattggagt
    15121 aaactcattg ccattataaa ctaaacggcc ataaatatca tcagcaacta tcaaaatatc
    15181 attttttact gcccattcac cgattgcctg cagttcattc ttgctataaa tcattccagt
    15241 cggattcgat ggagaattga tgattacggc ttttgttttt tctgaacgtg cttgttctag
    15301 ctgctcaaca gtaactttcc agctattgga ttcttctcct ttcaaaaaga caggaagtcc
    15361 ttctgccagt tttacttgct caccgtaact gacccagtaa ggcacaggaa tgatgacttc
    15421 atcttgcggg tcaaggatcg tttggaaaag ggtatacaaa gcaaatttcg ctccgtctgt
    15481 tacgactgtt tgtgaaggtt catagacaag tccatagttt ttcttcagat aatcaacgat
    15541 tgcctgtcga agctctggga ttcctgctgt tggtgtataa tagctagctt tcccattgcg
    15601 gattgcttcg attgctgctt cttggatgtt ttctggtgtc gcaaaatccg gttctcccac
    15661 agtcaaactc agcacatctc tgcctttggc ttttaatgcc ttggcctttg ccgcagctgc
    15721 caaagtgaca gacggttcta atcgttttgt tctttttgat aactccatcc cattcattcc
    15781 tttacctttt aaattccctt gattgtcttg acttcatctc cagattcaaa gccaatcagg
    15841 taataattca gtttcccatt tgtttctttt cctactactt cccacacagg ctgatcatca
    15901 tacatcccta aagcagcttt ttcgatttgg atttcaggat gggccgcagc aattgtctgt
    15961 tttgcttcat tttcagtcag tccctttgtt tgatccaata cttttacttt ttcaccagat
    16021 ttaggaatga taacggcgat ttcttttcct ttatcatttg tacctgtcag tgaaaagtat
    16081 gtcttctttc gagtaaacca ataaaaatga tcggcttcct caagatttgc atatttcttt
    16141 gcgatttcta ctgcttcttt cttcgcctgc accgccggac gactggaacg caagtaaaaa
    16201 atcgagctga tcacgatgac cgctaataaa aatacgatca agccaagtaa cacgcgatta
    16261 atcgtttttt cctttgtctg ttttttcttc aagggtcgtc gctcctttat cttcattcct
    16321 tttcattata gcagatttta attatgaaaa aagacagctc ctctaacttc cttcttcatt
    16381 tttctttaag aattcatgga tatcctgata gatttcctcc atagctagct ctttgatcgg
    16441 tacttttttc ggtaatgcat ggatgatccg tgcaccatat tttgcagtaa ctagtcttcg
    16501 atccaaaata agcaatacgc cacgatctgt ctccgtcctt atcaagcgtc ctaatgcttg
    16561 gcgtaacttc aaagctgctt taggtacgga ttcctggtaa aaaggattga tcccctctga
    16621 ttctaaataa gcgttcctag ctttgaccat cgggcgaagc ggattatcga agggtaaacg
    16681 tgtgactaca acgatctgca aagcatctcc aggcaaatct acaccttccc aaaaactgtc
    16741 agcgccaaac aatacgcttt tttctgacaa aatgaagcgt ttcaaaagct tctcccggct
    16801 tccgccaaac ccttgagcta gtatttctcg tcctttttct agcatagaca gatgcatttt
    16861 ctgatagacc ttctgcaaaa catcatgaga agtgaataac acaagtattg gccgatcttc
    16921 ttgttcgatc agacctgtaa gtgctgtctc taaataatcg atatatgctt gattgctgac
    16981 ttgatggacg gcaggtgcct cttttggcac atagatcctt gcctgtttct cataatcata
    17041 aggagaggca atcactttaa gaggtgctgc ttcaatgcca agccgcttag agagatagtg
    17101 tcgattaggg ccgatcttca gcgttcctcc aatataaaga atacgatcat atcgaggata
    17161 ccaaacagtc gacggcaaca tagccgcttg aaaatcatgg agatgaaaaa tcatctgctg
    17221 cgctgtgttc ccaggaacga accaatggat caaatgatct cgccagtctt ccagccattg
    17281 atccatcagc aagtactgct tgtctaattg atcaaataag ctatataaac ttgctaaatc
    17341 gatccgcatc tggtttgtcc agctttcttg gataatctca aaagtcagac gcatttgtga
    17401 ctgtagctgc tggatctcct gataaaaaag ctgcaggtga tcaatggctg tcccagattc
    17461 cagtggcagc tgatccaaca tctcttttgt cgtcatgact tcttctggat actgtccgtt
    17521 aggttgcttg ccgctcacta aactcattaa tccttctgcc atacaaatct gtgcttcgca
    17581 tagcgcagaa agttcttctc gataaatcga taaccagcgt aaggcttcat gttctttcgg
    17641 taaatagctt tgtattcggt caaacagctg atttggttct tctaattgct ggatttgttt
    17701 atggaaatag ttaatactta atcgtttgtc atttactttt tcagcgattt ccggcaaatg
    17761 atgtacttca tcgatcaaca aaaatggact agctggaagc aatggttctt ttcgctgtgt
    17821 ttcttgtatt aaaaacgcat gattgacaat caaaaaatta ctttgcctga ttttcttttg
    17881 caagaaacga acaaagtctt gttcgtaaaa tgactgattt cttgctaaaa atccagtgcc
    17941 tctgtgcgcg atatctgcaa agaatgaatg gttcaaattt gttaaatgta attcatccaa
    18001 atcaccggtc ttcgtttgcg tcagccaaac taagaccgcc atttgataca aagcatattg
    18061 tttttgttct ggcggttcca caagtgtgcc tttaaaacgc tgtaaatcaa tatagtggcg
    18121 atagcttttg ataattacag gttgtatggg ctgatctagc aattgattca gcaatggaat
    18181 gtctttttct atgatctgct gctgcaaaac tagcgaaacg gtactgatga cagcgggttt
    18241 ttcaggtgta gccaaataac tcaatggcag cagataaccg attgttttac ccattcctgt
    18301 agctgcttcg atcatcaaat cttttgattc atcttttgtg aaatggtcgt agaccgcatt
    18361 catcagtctg ttctgttctt ttcgatacat cagtgtttta ccaaacattt tttcttttgc
    18421 ttgttttttt cttggatatg tccgctcgcc ataatggacg tatatgaata gttccacttc
    18481 ttttttctgc aaagctagcc catcgacgat ttctaatgag ggatcaagtg gctctggttt
    18541 ttctttcatt tcttcaatca catgataaat gaaacgactc gtatccatac ctgtgtgtct
    18601 acttaatcgc gcaatctgct ctaacgtgat gattggcaat tttctcatcc gttcttcgat
    18661 cagcaaaaga agttgacccg tcacttcggc atcactatct gcctgatgag gattctcatg
    18721 aataaatcca aggctttctg acaaatcagc taagcgaaag cttggttccg tcggcaagaa
    18781 aatttgtgca agttctactg tatcgatgcc tggtatcttc aaactaggaa gtccgcatcg
    18841 catgagttca tggttcaaaa aattataatc aaaataaata ttatgcgcga caaatatggt
    18901 gtctgccaaa agattgtaaa tcgtttgggc gacatcttcg aaataaggcg ctttttggac
    18961 gcgttgattc gtgatccctg ttaaatgctg gatttgtttt gaaatcttcc gccctggatg
    19021 aatatcgatc gaaaaacgtg aagtgatccg acctgattca atcatgacgc agccaaattg
    19081 gatgatccga tcttcttttg gattcgtccc agtcgtttcg atatcaacga ctgcataagt
    19141 tcttggtttc aaaatatctt cctcttctcc tgcctctaat gttcttttga aattatacta
    19201 caaagtgcag aaaaatactc tcatcgcttt ttaaagaaaa tagacaaaaa cgcccagaca
    19261 cgcaaatgtc tgggcgttta ttttttagta taggttaatt aagctttagt aacgtttgtt
    19321 gcttgaggtc cgcgttggcc ttcttcaaca tcgaaagtta ctgcttggcc ttcttctaaa
    19381 gttttgaatc cgtcgccttg gatagctgag aaatgaacga atacatcgtt tccgttttct
    19441 gcagtgatga atccaaatcc tttttctgag ttaaaccatt ttactgtacc tgtttccata
    19501 ataaaaatcc tccttgtgta tcaacacata ttttgcaatt acttgaatgg tgtatatctg
    19561 gaagatgttt atttgaaaca agctaccaca gattaccgaa caaaaactac tgctatagta
    19621 tatcataccg tttacaaaaa agctagagtt tttcgacttt ttttattaaa aaaagttttc
    19681 tcagcgatta cataaagtga aaaaccgaac tttttttaaa ataaacgaca tattgttcag
    19741 ttggtgtttt tttgctctga tgttcttctt taaactaaaa aaattattct ttctgactac
    19801 ttatcggttc tgtttcaacg gtcgaggctg gggaagattc tttagtaggt ctgatgttct
    19861 catggtattt ctgatagcct atgacagtta ccagaagaac aaggcaaacc aatacgatca
    19921 acgagattag tttttctttt ttgctcatat ctatacctca taaataacag gatgatgtcc
    19981 atcatatttc tgagtatgag taaaagcaaa agctgcagct gtagaatgta agaaatgcca
    20041 gcaactttca taatcacagg tcggacagta ccaaaagtaa ccaaatcttg gctgcaaaga
    20101 ccgttcttct gtttcaggaa tcgcagaatc aagatctgtt gtctgtacat aaacattttt
    20161 gtacagacgt gtgcctgtaa taggagaccg ctgtgtttct tcagcaaaaa ctggctgact
    20221 tgttaaactt agggatagaa acagcaaaca ccataacagt ctttttttca taaagacctc
    20281 cttatcgata agtttttccc aattaaaatc atatctcaag aacgcccgtt attcacattt
    20341 tgttcataaa tgatgttttt tgtatcataa tcgtcaaaaa gaggtcgtga ccacgtcaca
    20401 acctcttttt taaacggtgt tccagaagta gcttcttcgg aaataagtcg aaatttcaca
    20461 aaaatttgag aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt
    20521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtattag ctcaaaaaac
    20581 ggaaaaatat gtgaaactat ttttccgttt gtttctctaa tactcaggtc aaaacactga
    20641 tttcacaacc tcttttaata acgaagaatc gctgtcagac tcttttcatc tggcgcatct
    20701 ttttgatcca aaataaacac tttcccgcca ttaatgatca catcatctgc taatgcattc
    20761 aacacttgac gccgatcata ttctgtttca ggatcttcac cgaaaccatc agctaagttt
    20821 gacgtagcga taaataaatg tgacactttt ccttctttcg cagctggaac gatatcaact
    20881 aactgatcga tgaatttctt atctaagagt ttattgtatt cttttgattc ttgagctgcg
    20941 agttcacttg acagcttctt cgccccttct tcgatatctc taaaggatag ctgcgcagga
    21001 gaagcagaaa tcgcaacgtc tgcacaataa tagggatttt tagcaatttt tttgaacatc
    21061 gtctgatttt ctggtaatgc aaataaataa atcggccatt gttcaggatt ttcgaaagaa
    21121 tccttcaagt acgtatcaac agcttggtaa tagttgaccc aatcgatctc tacttcttca
    21181 tcttttggat ttacgccatg gaaagttact ccatctcggg ctccgcctcc tccattagaa
    21241 gagaagttca aaccgccacc tgtcagttca gatcccaatg cagtaacgac atcaaccggt
    21301 gccccttctg gtagagatac ttcttttact tgtttgttgc tgacctcata caatttcatg
    21361 gagtcgcggt tcaatcccaa taaataataa gaatagttga actgcccatt tttgataata
    21421 gccagtaaat aaggaagatc acttacatag tattgatcgt ctaccgggat actcaaacga
    21481 tggatatagg tctggtcagc agttataatg accgctacac tcgtagttgc actacgccaa
    21541 aacgaaggat ctcctaacaa attttcaatt ttttcttgga acggggacca gtcatgttcg
    21601 gaatattttt tctcaaaacg tttctttgct tctttcgcaa aatttttgaa tttgatctga
    21661 tctttttcca catcttgatg ggcgacatgc gtattcagca tgatcgtgac gaatggtcct
    21721 ttagcgcctt ctgaagtcaa ttgtgtcaat aattctttat ccttttttgc cataaacgta
    21781 cccctcctaa aaaaatcatt cctttgtttc tatctaaagt ctaccacaac actatttttc
    21841 catacaataa aacgtatcta aaaaaataaa acggtttcta cggttttaaa tttagctatt
    21901 tttttaagtt atcttcataa tttcctcaat tatttagccc cagtcttcaa ctattcttca
    21961 tgaaatgctg ataaactaag aaaaagaata aaagaggtgg atcttatgca tttgattatg
    22021 attttcagtt tattaagtat tatcagttta tttgctttca gcgcttcccg cttattagat
    22081 gcggaaacac agcctattcc tgtaaaagta cgtaaaaacg accgctaaac ttccataacg
    22141 cgcgttctta tcctaaagtt ttttcagaaa cattgaaact ctctcccctc tctgataaaa
    22201 ttttaatttg tagcgaaaca aattaattac aaggagatcg gatatgtcca taaaaaaaac
    22261 acgtctagtc ctattattgg ctagtctcat tctttgcctt gcttcttttc taccagctgt
    22321 tgctgtgcaa gctgaagata cgttcaaagt aaatgcaaaa gcagcttttg ctgtagatgc
    22381 gcaaagcggt aagattcttt atgatcaaga tggagaaaaa accatgggga tcgcttcaat
    22441 cacaaaaatc atcggtcttt acatcgtcct tgatcaagta aaagaaggaa aactgtcatg
    22501 ggatgataaa gtttcgattt cagattatgc agaaaatttg agtatcaccc cagatttatc
    22561 gaatgttcct ttgcataaag aaaacaccta cactgtcaaa gaattattcg actctgcaat
    22621 catccagtca gccaatgctt caatggtggc cttggcagaa aaaatctctg gcagcgaagc
    22681 taaattcact gaacgaatga aagaacaatt gaaagattgg ggaatcaaag atgcaacaat
    22741 cgttaatgca tcaggattga acaactctta tcttggcgaa aaccgaccag aaggcactgg
    22801 agaaaacgac gaaaatcaaa tgtctgctca agatgtggcc atcgttgccc gccatttgat
    22861 cttggatttc ccagaaatac tggatgtatc aagtactacg acccaaatgt ttggcgaaaa
    22921 tacgcaatca ccagtggaaa tggtcaactg gaactggatg ctcccaggat tcgttaatta
    22981 caaggaaggc gtggacggat taaaaactgg tacgactgag cttgcaggtg cttgtttcgt
    23041 tggtacgatc actaaaaacg atcaacggat catcactgtc gtattgaatg cggacggcca
    23101 tgcggaaaac ccgagtgccc gcttcaatga aacagaccgt ttgatggact actgctatga
    23161 caattggtca gaaaaagagc taggaaaagc taacgcaagt attcctagtc taaaaaccat
    23221 tgatgtaaaa gacggaaaag aatcaacagt tcctgttgtg cttaaaagcc ctgtaaaagt
    23281 atgggtacga aacgatatgg atactggcaa attaacgatc acgccaacac ttgacaagaa
    23341 gcaagtcact gacaatgaga tccaagcacc tatggataaa ggtacaaaaa tcggtaccgc
    23401 tacgatcacc ttggcagacg ataaattagg ctatttagaa gacaacaatg caccttcaac
    23461 agagatcatt acggataaaa cagtggaaaa agcgaatata ttcgtgattg gctggcgacg
    23521 cgtcgcagaa tttttcggca acttgtttta attgaacaat aagaaaaaca tctttccatc
    23581 ggaaagatgt ttttttgctt tttctagttt aaataacagc ttttttcatc tattttcgtt
    23641 atactgtata agaaaaagga gttgaattta tgaatctaac cagagatctg atctttttag
    23701 ttctctatct tattggggta ttctctatcg tttattggcg aaaacagaag aaggatcctt
    23761 ccttgttctt ttacggcagt acgtggacat ttacggtaat cttaacttat tttgtcgtgt
    23821 tggaaatttt ttattcgacc ttttattttt tgattccatt gttgttcttt agtatctttg
    23881 ctttttctta tttcacagaa aaacgaaggc tgctgaatgg tcttttattc aatgttttcc
    23941 tcatcagttt cggcatctac ttgtttgtct tgctgtatga aacgcaaaat attttccttg
    24001 gcggactgat cgccttgatc acgattccat tgctacttgt ccttttattt ggtatttatg
    24061 gattgattgt ttttcttttt tggaacgggg tcacagtttt acgtagagaa tcgcattcat
    24121 tagctaatct tttaacgttg atactagcca tttttcttac actgtttctt gtttttgact
    24181 ttttcttgtt aaaatatttg ccgcaatgga taaatgcttt gtttttctgc gttcccctga
    24241 tcttgatcta tctatttatc gtattctaca actttttgac tgtctcattc ttgtatcagt
    24301 tcaaccggcc tcgatacaat caagacttca tcgttgtgtt aggcgctggg ttgatcaatg
    24361 gagaaacagt ttctcctctt ctagccaagc gaatcaataa ggcaattgct ttttatcggg
    24421 ctcaaagccg tgctacatta aatccgccca tcttattgat gtctggcggg caaggagcag
    24481 acgaaaaagt accagaggcc attgcgatga aacaatatgc catggaacaa ggaatccctg
    24541 aaagagacat tcttgtcgaa accaattcga caaccacttt ggagaacatg ctctattcaa
    24601 aggaaatcat ggatcagcaa atgaaagggc cataccgcgc gatctttagt tccaacaatt
    24661 accacatttt tagagccggt ctttatgcgt ggcaagccaa attgaaagca aatggtattg
    24721 gtgcaaaaac agctttctac tacttgccta atgcgttttt aagagaatac atcgccatct
    24781 tgatgatgaa caaaaaaaga catatgatca tttgcggatt gcttttcgca ggctccgcat
    24841 tcttatcgct gattacttta cttttctaaa aaaacaaggt gtgacaaaac atatgtcaca
    24901 ccttgttttt ttctttcatt ctcttaacta ttggcaaaaa gagtatccct tttttgaccg
    24961 ctacttgctg agcagaataa ataccgctgt tgcctgaaca cataaagctg atcaagcaga
    25021 tcataaagaa gtatactgcc gcttgtgaac cgaacaattc gatccccatc acaaagcagg
    25081 caatcggtgt atttgtcgca ccagaaaata ctccgataaa gcccaatcct gcaagaaaag
    25141 gaacagaaac atggagcaaa ggcgctagtg ctgcaccaag cgttgcacca atttcaaaca
    25201 atggtgtcac ttctccccct tgatatcccg cgcctaaaga caaaactgtg aagaataact
    25261 tcccaataaa atcatagaca tgtgcctgtc cattaaatga ttcttgaagt aacggaagac
    25321 tcaatccgag ataacgttgt gtttgcaaaa tcaatgctgc tgccacaact acagctccac
    25381 ctatcgtatt tcgccaaaca ggatgaggaa tccattgtgc atatcgcgct tttaaaaaga
    25441 cgatcgaacg gctgaatacc caaccaatca aaccaaaagc aatcccagca acaaaaactt
    25501 tgaagaacag caacatactc cattcaggaa tcacacccat ttggtaatgt agatgagaaa
    25561 caccatagct ttctgtcatg aaatttgcaa acagtgcagc aaagaagcta gggaagatcg
    25621 cttctgttcg gactttcccg attgccaaca cttctaatcc aaataatgtt ccagctaaag
    25681 gtgtgccaaa tacagaacta aaacccgcac tgattccact gataatcaag atttcccgtt
    25741 ctgatttatc caatcggaac aaacgtgctg tattatctgc taacgctccc cccatttgga
    25801 ctgctgttcc ttcacggcca acagatcccc caaataaatg ggtagttatg gttccaaaca
    25861 gtgtaagtgg aataagtcgc aacgggattt tttcttctcc accatttcct tgatcgatca
    25921 ctaagttgtt tccacgacta gaaagaccgc cgtgatacgc atagagataa gcaaaaagcg
    25981 caccactaac tggtagtaga taaaggagcc aaggatgtga tagtcggata tctgtcacaa
    26041 ctgtcaggct atttaagaaa aaagcagaca agctccccat gaatataccg agaatcgctg
    26101 tgatcaccag ccacttcagc atgtacacta aaatgcgtac tggactcggt aattgttttt
    26161 ccataaaaaa ttcctcctca ataaaaaaac gcctactaaa gaaaggggct gtccccagac
    26221 tttagtaggc gtcattagct atctttcgtt agcatttaaa ggcgaacacc atcacctatt
    26281 cacttccatt aaaagatagc atatttttct gtaaaaaaca ataaaattat cgttctttct
    26341 ttacacgttc agcaaattca tagaaaggag ccaatttcgt taactgttga ttacattggt
    26401 cactatcatg acaaatataa ttcccctttt tcgtatacgt gccatcagaa cctgctttcg
    26461 ttgtagaaag aaaaagcgaa acattcgatg ttttctgaca gattgcacag attcccttat
    26521 ttatcgttgg tgataaagta ccactaatcc cttttaattt ttcatcttcg taatagatca
    26581 tgaatttttt ctgcgtacct ggatcattcc agccgatgta cgtatgttct tgcaagtcga
    26641 tttcattcca agctggttgt ttgagttttt tcacttttcg gaataatttc tcgatttgtg
    26701 gtttgctagg ctgcataaac ggctgaacga tttgcttcag ctcctccaat gctcgttctg
    26761 cttgcgcttt tgtcagtttt acgtctaaaa ctgtcgttaa aaatgtatcg atttctggca
    26821 gttcgtgttc taatatgtct cgaatctttt cagttgctag tgcctgaact gtttgtaccg
    26881 tttttggatc attgacagat gagtacgcat ttaataactg ttctacttct ctttttataa
    26941 aaaaatattg gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca
    27001 aagtgcatac tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc
    27061 ggactctttt aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattct
    27121 tctatgtaca tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac
    27181 tgcaaacaga aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat
    27241 ggtgttcaaa agcttgcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagta
    27301 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg
    27361 gtctattatt ggttttacct gttaaatcga ggattctttc ccaaagtaac gttgccatct
    27421 cttacgcata cttgcagaca tgggctgagc cgcttctttg actgcgcagt atttgtccac
    27481 gaatgtcaca atatatcctt ctttatactt aggcggtgcg atcgttgcac cccacatgtg
    27541 cttcaaaatg atatctcgct caagatccga caaatcggtg attttttctg cattctttac
    27601 agcaattctt ggatggatat acgcatgtgt cccctcgtca aatttcgtcg tacgccaatc
    27661 atagtagaac agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca
    27721 tttttttgcg agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga
    27781 atgaacatgc tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttg
    27841 tacataagac atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc
    27901 acctttttga atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt
    27961 attctagtcg ctctagataa aatagctaaa gcttaatagt tccttaatca taaagaaaaa
    28021 tgataaagaa cgatccctgc tgcgactccg acattcaatg attccgcatt tcctttcatt
    28081 gggatatata cgttctgatc agtcaaagac aagatttcct gacttactcc ctgaccttcg
    28141 tttcccatga ttatagcgaa atcctctgtt ttttctactt tgtctaatgc atgtgcttct
    28201 tcatttaatt ctgtaccgta caccgtaaac cctttgcctt tcattacagg gatcacttct
    28261 gccaaatctt tttgtaacac aggcaaatga taattgcttc cctgcatgct tcgcagcacc
    28321 tttgtactat aaatatctgc cgttccttta cctaataaaa caccagccat accaaacgca
    28381 tctgccgtac ggatcatcgt tcccacattt ccaggatcct gtacattatc tagtaaaagc
    28441 cagcctcctg aaaaatctgg cgtatcttca gatgttaacg tcatcaccgc taagatccct
    28501 tgcggtgtag gtagatctga catagcggtc atgacctcat ctgaaactag aaaagtagaa
    28561 atctctttgt ttacagccag ccagtctccc cattctttca tacctctctg tgtcacaaaa
    28621 agttctttta tttctgcatc agacgacgca gcttcttcga tcagatggaa cccttccaaa
    28681 agatattctg ccttttcttc tctgtatttc tttttatgta gcttttttaa ttctttgatt
    28741 tttggatttt ttgctgattg gatctctttc acattccttc acctcacctg attatatcat
    28801 ggaataggtg cttgagcttg tttcatccta aaaaaatcgg tatcattgat ttataaaaca
    28861 ggaaagaaag gtgacgaaca tgcgcaaagt taaaatgaat gtccaaggac gtgtccaggg
    28921 cgtgggattt cgctatatga ccaagatggt tgctgatcag ttaggcgtga ccggaacggt
    28981 aaaaaacgaa gacgacggct cagtcacaat cgaagcaatc ggaaatgacg acatcatcca
    29041 aaaatttatc gaagaagtca agaagtctcc gagcccaagt ggacgagtgc agtatgtgga
    29101 tgtccaagaa gatccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg
    29161 tgaaaaggtc gcttagtttt gagtattaag gaaaattttg tgaaaatggc tttccacatt
    29221 tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgtcg atcaggttgt
    29281 ttttgaggcg ctttgcctca agtattaaag ataggtagta aaaaagctga tctataactt
    29341 gcaataaatt tgcttaaccc atattttcca gacagaacaa aaaagttttt agtt
//
LOCUS       DAZUMV010000021        27774 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000021 DAZUMV010000000
VERSION     DAZUMV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.021"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(323..1267)
                     /locus_tag="ACG8FK_000865"
     CDS             complement(323..1267)
                     /locus_tag="ACG8FK_000865"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5096494.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1563..2507)
                     /locus_tag="ACG8FK_000866"
     CDS             complement(1563..2507)
                     /locus_tag="ACG8FK_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5096495.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2728..3942
                     /locus_tag="ACG8FK_000867"
     CDS             2728..3942
                     /locus_tag="ACG8FK_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5096496.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3964..5979
                     /locus_tag="ACG8FK_000868"
     CDS             3964..5979
                     /locus_tag="ACG8FK_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS5096497.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6062..7435)
                     /locus_tag="ACG8FK_000869"
     CDS             complement(6062..7435)
                     /locus_tag="ACG8FK_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5096498.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7461..7745)
                     /locus_tag="ACG8FK_000870"
     CDS             complement(7461..7745)
                     /locus_tag="ACG8FK_000870"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5096499.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7747..9762)
                     /locus_tag="ACG8FK_000871"
     CDS             complement(7747..9762)
                     /locus_tag="ACG8FK_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5096500.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9941..10246)
                     /locus_tag="ACG8FK_000872"
     CDS             complement(9941..10246)
                     /locus_tag="ACG8FK_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS5096501.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10335..11366)
                     /locus_tag="ACG8FK_000873"
     CDS             complement(10335..11366)
                     /locus_tag="ACG8FK_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5096502.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11573..12448
                     /locus_tag="ACG8FK_000874"
     CDS             11573..12448
                     /locus_tag="ACG8FK_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5096503.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12600..13442)
                     /locus_tag="ACG8FK_000875"
     CDS             complement(12600..13442)
                     /locus_tag="ACG8FK_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5096504.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13534..15411)
                     /locus_tag="ACG8FK_000876"
     CDS             complement(13534..15411)
                     /locus_tag="ACG8FK_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5096505.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15408..16829)
                     /locus_tag="ACG8FK_000877"
     CDS             complement(15408..16829)
                     /locus_tag="ACG8FK_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5096506.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16832..20221)
                     /locus_tag="ACG8FK_000878"
     CDS             complement(16832..20221)
                     /locus_tag="ACG8FK_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5096507.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20478..21869
                     /locus_tag="ACG8FK_000879"
     CDS             20478..21869
                     /locus_tag="ACG8FK_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5096508.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21942..22589)
                     /locus_tag="ACG8FK_000880"
     CDS             complement(21942..22589)
                     /locus_tag="ACG8FK_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5096509.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22597..23283)
                     /locus_tag="ACG8FK_000881"
     CDS             complement(22597..23283)
                     /locus_tag="ACG8FK_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096510.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23289..24362)
                     /locus_tag="ACG8FK_000882"
     CDS             complement(23289..24362)
                     /locus_tag="ACG8FK_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096511.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24584..25258
                     /locus_tag="ACG8FK_000883"
     CDS             24584..25258
                     /locus_tag="ACG8FK_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5096512.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25263..26603
                     /locus_tag="ACG8FK_000884"
     CDS             25263..26603
                     /locus_tag="ACG8FK_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5096513.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26752..27738)
                     /locus_tag="ACG8FK_000885"
     CDS             complement(26752..27738)
                     /locus_tag="ACG8FK_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS5096514.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8094 a         5251 c         5042 g         9387 t
ORIGIN      
        1 tacttttttt gtcgtttaat tgaatgataa aaaccagttt ctttattcag ttaatcaaat
       61 atgaacgaat tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc
      121 acagataaac attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc
      181 atatgtacgg atacattttc ggaccgatta attttaacca ttctgtttat ttgcttacct
      241 attagatatc aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttaggatt
      301 tttagttcat attcattgtt tcttataata cagtgttcaa tacttctctt aaagcacttg
      361 ttgatttgct gaacattgct ttttcttctt cgttgattga taattcaaca acttcacgaa
      421 caccattttc gtcaacgatt gatggcacac ctgtaaagat atctttttcg tcgtattcac
      481 cagttaaata agcagataca ggcaatacag cttgttcgtt attcaagata gcttttacga
      541 tacgtgcagt actcatacca ataccatagt aagttgcttt tttacgatca atgatctcat
      601 aagcagcatt acggacttta tcagcaatca catctaattc atcttgggca atacgatggt
      661 ctttttctac aatttcaaac actggtttac caccgacagt tgtatgtgac catgcagcaa
      721 cttctgaatc accatgttca cctaagatat agccgtggac gctgcgtggg tcaactttta
      781 atttcaatgc gatttcttta cggaaacgag ttgtatccaa tgttgttcca gtaccgataa
      841 cacgtgatgt tggcagacca gattcgttcc aagcgatata tgtcaaaata tctactgggt
      901 ttgaagcaac aacgataata ccgtcgaatc ctgatcccat gatttctttt acgatttggc
      961 gcatgattga cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt ttttggttga
     1021 taccagctgt gataacaacg atatcagcat ctttacattc ttcatagcca cctgaccata
     1081 cagccacatt ttcatcgccc caagccatac catctaaaag gtccaatgct tccccttctg
     1141 ctttttcttg attcacatcg atcaatacta attcattaga gattccttgg ttgatcattg
     1201 cataagcgat acttgttcca acaaaacctg taccgacgat tactacttta cgacttgttt
     1261 ttttcatgaa taaacagctc cttttcataa ctatttgtta ctttcatgaa aagtatagtc
     1321 ttgtttattc attttatcaa catgttaatt ataatagttg ttattataat ttttgatgga
     1381 aacgaattcc tgaatacgca taacagataa atgacaaatg tttacaccta tatcccctga
     1441 taatacaacc aaaagtttag aactgaaaat aaatccaaaa aaattcgctg caacgatata
     1501 ataagacagc aatcgcacct aaaacaattt tccttcatat gctttagtga aagattttct
     1561 tcttaatgtt tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg
     1621 aataaagaag tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc
     1681 gtttcttgaa tcagttccaa cgcaaagtcg aattttttct ttagaaccag ttcttgaaag
     1741 gaagcatcaa aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga
     1801 gctgctgttt cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta
     1861 agcatctcat tttctgcgca tccatctgct gatgaaaagc tttgttgttc taataaatga
     1921 gtcaattcga tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat
     1981 gtatcaaaat aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg
     2041 ccgcgaaaat gaatgaattg tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa
     2101 gaagaaagta gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct
     2161 tcttttaata agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta
     2221 gcttctttat caatgatcac cacgtcactt tcttccatct ctatcctttc tccattaata
     2281 atttgtaaac atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg
     2341 ggaacttccg catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatcc
     2401 cctcggtgga tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc
     2461 attccttgtt cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa aaatgtctaa
     2521 ttcagctagt tccaattgac tccctccttt ttgtaagcgg tttctttcgc ttattagttc
     2581 acgctgtact gctattatat gcaaagctat ttttggtttc ataaagactt tctatggttg
     2641 ggacatgaac ttcctgtgat tccgctattg agagaataac cacttcatac tatatttttg
     2701 ataagcgctt acaaaaatga aaggaacatg cttatgaata aaaagatgat gaaattttct
     2761 ctgctgtcag tctctcttat tttgacttct gctggtgcag tcacgtctgc aattcccagt
     2821 atgtcagcta cttatgccac gcgttcttta tctgctgttg aattattaac gactgctccc
     2881 tcttttatgg tcactatttt tgttttgatt agtagtttta ttgctaggaa aatcggacaa
     2941 aaacaaactg tgatcatcgg attagtgatt gcatcgattt gtggtatcct ccccctcttc
     3001 tcacaaagtt acagtattgt cttagcttca agagctggac taggtattgg ctttggattg
     3061 atcaattctt tggcagtcag catgattgct gccttttatc acggagatga acgagcctct
     3121 atgattggtt ttcaaagtgc atttcaagga tttggtgcag ctttaatgac atttgttgca
     3181 ggacagctag tacagttcgg ttggcaatat acctttttag tctacgccat cagcttacct
     3241 attcttgtct tgtttgtttt gtttgttcct acacctgaaa caactgaact tcaatcgcat
     3301 ggcacagccc caacaagaca agtaatcaaa acgaatccta aaatgattag ttatggtatt
     3361 tttctcttgc tggtcatcgt ggtatataat gctgtatcga ttaaattagc cacagtcctg
     3421 acgacttatt ctctaggaaa cgaaactaat gcagcgaata tcttaacaat catgcaattg
     3481 gcaagtatgg ctgccggagt ttgttttggc tggcttcaga caaaagcaca tcgctatatg
     3541 cttacacttt ctttactact gatgggaatc ggctttgtat tgattgccac tgtgataaac
     3601 ctgtatgtga ttgggtttgg tgcgatccta acaggaattt ctttttcgtt attcattcct
     3661 tacttattca accaagtaag tatccaagcg cccaagtacg cggagagttt caatacttca
     3721 atcttattgg ttggcgctaa tttaggtact ttcattgcac catatggttt gctctttctc
     3781 tcttttgctt ctcttgggag taaaacactt gctgttttcg tcaatggcag tatcttgctg
     3841 ttgattagcg cgcttattag ccttctagta gtacacagga cgaatagaaa gacagtgata
     3901 agagtagatg aagaaataaa agaacaattg acaacaaaat aaatttgatg gaggtcgtgt
     3961 aaaatgacaa atgaaaaaca taaagataga tggctttggt atcctggaga ctttgagatc
     4021 cgccatggac tgctacaaaa tttccaacgg gaagaacgag gttttgactg gccagcgtac
     4081 tggtacatgg atgattgcca tcggaacgtc aagtttaaga gatattactt tcttgatcaa
     4141 ccttccatgt ttaaagtgac gatccaagga gttgggtatg tagaaattaa tggacaaaaa
     4201 catccatgtg gaaaatggct gacttgtcca gctggtaaag caaaaattcg tatctttgta
     4261 ggacatacat cagggctacc agcgatgttt atcgagggag acgaagtaaa atcagatatt
     4321 ggctggacag caagtaattt tattgaagaa tatccagctg gctggagtcc gctttatgtc
     4381 gatatcgcaa aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca tccagtcact
     4441 gaaacggaga ttggcggagg aatcttattc gattttgggc gagcagtgaa tggctgtgta
     4501 gaactaatat ctctggatga ccatatcgaa tctatcactc tatgttatgg agagtccgat
     4561 gtagaagcta tggatatcga ttattgctac tataagcaaa taggaatgac aagaaaaatg
     4621 aaagctcgta aacgcgcttt tcgctatttg tttataccag aggtttctac tggaatggtt
     4681 caacttcaag tttttcatga atttttatct ctttcgaaac aagctgcttt ttcaagcgat
     4741 tcttctttac tgaatcagat ctggtcagta tcagaagaag ccttcaagtt atgtagtgat
     4801 ttgtttttta tcgacggtat caaacgagat cgctggattt ggtctggaga tgcgtatcaa
     4861 tcattcatga tcaatcaata tctttttttt gacgaggaaa tcaataaacg aacgatatta
     4921 gcattaagag gacaaaatga aatcaagcag catatgaata ctatcgttga ctattcgatc
     4981 ttgtggttga ttggtatcga aaaccactat atgatgacga aagactatga atttttacgc
     5041 atcgtctacc ctaagatgca atcattaatg agatacttga tggagcaaac gaatgaatta
     5101 gggtttattt atgggagagc aaaagattgg atatttattg attggtctga aatggataaa
     5161 gaaggaacgc ttgctgcaga acaagtctta ttactgaaag cctatcattc catgatcact
     5221 tgcggagata tcttagctga aacatatgga gagaacgaaa attatcatac tcatctctat
     5281 caagaaaaat atcaactatt gaaacaaaac ctatttgact acttttggga tgaagaactt
     5341 ggcgctttta tcgattgcta tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt
     5401 ttcgctgttt tattcgattt tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc
     5461 ttattgaatg acactatcac acagattact actccatatt tcaaattcta tgaacaagat
     5521 gctttatgca aactcggaca gacgaatatc gtctaccaaa cgattcttga ttattgggga
     5581 gggatgttat ctcatgatgc ggtaactttc tgggaagaat acgaccctac tcaaacagga
     5641 aatgaaaagt acagtatgta tggagatcct tatggaaaaa gcttatgcca tgcatgggga
     5701 gcaagcccaa tctatctttt aggacgctat tttatcggtt tgtatccaac aaagccaggt
     5761 tatgaagaat ttatgataca acctaatcta gattcatttg aaacactgaa ttgtacgcta
     5821 cctatcaaag atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt gattacttct
     5881 gatagaacag gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag
     5941 ccacttgtct taacaagcta ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca
     6001 tatcagaaga ctttaaatct tcttctgata ttgtccaagc aatttttatt ttgaagtttg
     6061 cttaattctg ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga aagctagata
     6121 atacgacaag taaaaagaaa gcgatgattc caactgtcaa ggctatttga ccgtattctc
     6181 ctagtttccc aagtacaagt cctggtacta gataatcagt atcggcaaat gtagaagaag
     6241 cgattttaag atctcctaat actggaagga taaataatgg cagccaacta atcaataagc
     6301 tattgaaagc agaacctaat accgcccctc ttctaccgcc agtcgagttg ccgaagacac
     6361 cagaagcagc tccgcagaag aaatgtccta ctaccccagg aatgataact gtagtttgca
     6421 agcctatcat agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa aagccaatta
     6481 atacagcatt aggtgcatat ggaaaaacga taggaacatc taatgctggt ttagaatcgg
     6541 gtacgagttt tttagcaatc ccttggaatg ctggaacgat ttctgccaac accattcgaa
     6601 ccccttgaag gacgataaca aacccagctg tgaatgttcc agcttggatc agtgcaaaga
     6661 ttacagcgtt gtctccattg cttagttctc tttcaacaaa tccaggacca gctaacaaag
     6721 caagtgcgac atatacgatc gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa
     6781 agctgaaact tttaggaatc tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact
     6841 ttccgatcca tccactagaa gcataaccga tatttccggt atgccctaac gcaacttgat
     6901 tgtttccagt gatgttcttc ataaacggtt gacaaagagc aggcgtaata gttaatagtg
     6961 ttccttcgaa gaccccgcct aataagatca tccacaaatt tgcagtaaac cccgcgctta
     7021 ctaaaatgac tgctgttaga caagaaacat aaagcattgc ttgacctgta agaaaaatga
     7081 atttatatct tgtgaatcta gcaagtaaaa tattcacgat catccctact agcatgatca
     7141 aagcagtagg tgtaccgtat tgttgcaaag caatcgccac tactgcttca ttgctaggaa
     7201 ctactccttg agtatgcaga gctgtttgga acatcgaagc aaaaggggtt agtgaattac
     7261 tgattaaacc agcaccgccg atcagtacta ggaatccgac aaatgttttg atagttcctt
     7321 ggatcacttc tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa
     7381 cgaggatgga gggctgactg acgattgaca caaacaaatt caacaattta tccattgata
     7441 gttcctccct tgaatcattt ttattctttt gacaagatct tcgttacttt ttcttttact
     7501 tcttcgctgt caatgatcga ttgcaaaggt acgagctttt catcaggtat agaagttagc
     7561 gtatctttca aagaatgctc aatgaagaaa taatcagcca tatcgctaga tacacttcca
     7621 acatccgtat gacttacttc aaactcgtcc acatctgctc caagatctgc aagtgcttgt
     7681 ttgatgttca tctctaccat aaaacttgtt cctaatccac tttgacatac tgcaaaaatt
     7741 ttcatatcat tcgtctcctt ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg
     7801 taataaatct tgaagatttt gtttattaga aagtagcttt gttaaatttg ccaaagcccg
     7861 aagatgtgcc tcattatcga tagcagctaa acagatgaac aaactaatgg gatgggctgg
     7921 attatcaagc agatccacag gttgttgaag tttcagcaaa gacatcccta cttcgtttac
     7981 gccatcttct ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc
     8041 atactgctcc acgcgctcga tcatagcttt gatataactt tcttctattt tattttctga
     8101 taatagcggc tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt gatccgataa
     8161 ttggatcatt tcttctgtta ataattcaga tagcatagga cgttgatcct ccttcttttc
     8221 taaaagtttg ttcattttac gatttaatac ttgatagagc ttttcttcgt ctacgccttc
     8281 ttttaattcg atataaggca gtagcgccga caacaattct tgggcagaag gcattgaaaa
     8341 tcctggtatc agccaatccc tttgtacttg attgatcagc tgattttttt ccaagtcagt
     8401 catgattgga tgcagaacat agagagactt tttcttatca gtaagtgtga gaggtacagt
     8461 agaaaaaata atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt
     8521 ctctgtaaaa aagatcatcg ggaataattg ctgaagttca ctttttaaga tcaatgaaga
     8581 gctaatgcca cttggacaga cgatcacggc tcttattttt ctatttcttt cttctgcgtc
     8641 cgcttttctg atctctccgc caaatagtat agtgaaaaaa ccaatctctt cttcagggat
     8701 gctcttccct attcgtcttt ctaacggaag taatgctttt cttgtcattt cgtagatgga
     8761 agcatattga tgcttaattt gatcgatcat cacattaggt aaataaaaag aataggaaat
     8821 acgaaaatag gcagggacca agtgataaaa gacattcatc aataattcac gaaacgaagc
     8881 aaattctacg gccatcagga attccatgcg atggatgact tctgaagaac attccagcaa
     8941 aaaatcaagt gcaggctcct ggacatctcc ttcaacgatc gatgctaagc aaagagagaa
     9001 aaatataatc tccgaatttt gatgttcgga atagccaagg ccagtcaata cttgtcgact
     9061 aatgtcagtg actgcaagag aagccaacag ttcagcatct tttaccagtt ccatggatgt
     9121 ttttccaatt cgaatggttg tcaaactcaa ataataagct aattcatcca ttcgatttgg
     9181 tgcgaatcga agatctgata gtgaaatcgt tttatctagc aaatctctaa ctttggcgta
     9241 ataatcaatg gaaagggttg cggttaaata cgtgagccca aatcttccag caagtgactg
     9301 catcaaacga gcagtgcgat tttttgcttc tcttcttaat attgcttctg accctagcag
     9361 cctaaaacca gttttcctag tatacgacag ctgacaatct tccacttcta attcatttct
     9421 tatctttttg atatctgaca ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg
     9481 aaaaacagaa acagacgttg cagtcgaata aaccatcaaa taaagaagat ctcttcgctc
     9541 ttgttcggta tacacagctt caaatcgagt aatatccaaa aatttttctg cccagtcgat
     9601 tgctcggaat ttctttatac ggatacgggt atcatccaca tattcggcaa aaggagttaa
     9661 ttgtttgatc gcctcgcttg tatttacttc gatgatctct gctgcttttt ctagactgat
     9721 ttctcttggt gttttcagga gataactaat tttgtagttc acagcgctgt tcacctccta
     9781 cttatattct agtatggtag cgcttttaaa tcgaggggtg tttggtacaa cttacagttg
     9841 gatttttttt acgactgtac cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt
     9901 gaccaaatgt ctcctgcttt tatgaattga tttcgtccga tcaatgatga tcatgtttat
     9961 tttttgaggt cagttcttca aatttttctt tgacatcttc aaccgctttt tcgatatctt
    10021 tttcaaagtg attgggctca ttaggctttg tttcctcttc tacagaattt gaaccagata
    10081 aagctgtttc tttattttct gtttcaccag agtttgtgcc agctaatgat tcttctttgt
    10141 cttctgtttc accagaatta gttcctgcta aagccacttc ttcttctgtt ttaggggcca
    10201 ttgaacctct taatcgtttg tgtttttcac cgtcataatt tgtcatgttg gttcctccta
    10261 gtagttctat actttttcat cgtacaataa gacagtttat gcgtcaaaaa acaaacccga
    10321 ttcttacaaa tatgtcatat ttttccttct gattcttgaa tgcttttgat tccttcttcc
    10381 aaaattttca tttgtaagat tgtttcttca tctttgatga ctttttctgc gccattaact
    10441 aatgtctcgt aaatcccttc gtagacacgg ctgtaatcac caacctcgga aattactttt
    10501 tcttcatgat aactcccttg ttcatctata taggtcaata caccataatg ttcgggcaaa
    10561 tcgataccaa agtcttcatg atcaggcatg taaaacattt tcagatgttc ttcttgtcga
    10621 tcttttgttt gtttcacgaa catccctttt tttccgtaaa cgacgaaact tggacgttct
    10681 ttgatgcgga aataactgga ttttatcgaa attttcatat ttccatagaa aaaatccaga
    10741 tcgaagtaat cattcattcg tcctttccct aaaagttgac gtacatcata gctgatatca
    10801 tctggttcgc cgaaataaga aagaacttga tctactgtat gacaagcatg tccatataaa
    10861 tagctgttcg cagtagaaaa ttcatgcgta ttttctggta cttccggtct gaaataatca
    10921 taatgcattt ctacttctaa aatatcccct aattttccgc tttcgattac tttttggact
    10981 gtcaagaagt cagaatcgaa ccgtctgttt tggtaacatt ggataaatag acctttttct
    11041 tttgctaatg caaataactc ttctgcttcc tttgacgttt ctgcaaaagg tttctctact
    11101 agtacattct taccatgttc gagtgccatt tttccatatt cataatgaaa ttgactaggt
    11161 gtagtgatga caactaattg gatctcagga tcatcccaaa gatcagacag ctgatcagta
    11221 tattcgatgt cttcgaatgt tttccacact ggttcagatg gggaaaaaat ttttttgata
    11281 ttgattttgt tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt actctttcca
    11341 ttgccaagat aagcgattgt aagcatgagt ggtgcctcct tttattgatg ttttcagtat
    11401 atcctagaaa agcgcagaaa gtatttataa tagctattct ggtaaaaaag ttcagaaaaa
    11461 cacttgttat cattcggttt ggtgaaatta atgttcaaag taaggataaa aatcaaaaag
    11521 atagtagcgg aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga tcatgcttat
    11581 tgaagaaaaa ctaacaaaac aagagttgtt taccacgaca gagaaacgga ttgtcgatta
    11641 catccgtagg aatatcgaag ctgcagtcta catgacgatt gaagaattag caaaagctac
    11701 gtatacttcc cattcagcta tcattcgttt atgcaaaaaa atgggattca gcggatttaa
    11761 agaattccgt ttcgagctgg ggcgggaagt tcatcaattg attgctcagt tcaatcaaac
    11821 gattgatcct aattttcctt tttcaagtga agatagcact cagcaaattg caaagaaaat
    11881 ggcggattta tcgatccagt caataaaaaa agcacaattg cagattgaac atgaagattt
    11941 agatcaaatc ggtaaagtat tgaacaaggc acagcgaatc tttctttttg caaaaggtga
    12001 ttctcaaatc acggcaagga aattccagaa taaaatggtc aagttgaata agtttttgat
    12061 catggctgaa gaatacagtg attcttcttg gaatgcagcc aatttgacta gcgaggattg
    12121 cgccattttt attagctaca gcggtcgcat ccatcattat gaacgaataa tgacctattt
    12181 gaagcatgta gaggccccta ccttgctgat taccggaaat cagcattcag aaatggccaa
    12241 acaggctgat atgtgtcttg ttatatcgca ggaggaatat gattttgcta aagtagcgac
    12301 tttctcctct caaattgcct tcgattacgt attaaatacg ttattctcag tgatatattc
    12361 tcaaaatttt gaagagaata tccgcaactt gaaaaacaaa caagaattga tgcaaaaagg
    12421 ccttcttaaa gaatatcgga aaacttaaga aaagatcctc atttagtaat aagaaagata
    12481 gtgtttgata ggctctcttt gatatcatca taaaaacagg ctgaagtttt ctttttcaaa
    12541 cctcagcctg tttgtttacg gaacgattcg aatagatata ttatagaaat gaggaatagc
    12601 taacttctgt ttcttatttt gaacgattga cgaaaaagat atataagaag gaggaaaatc
    12661 agtatacatg cagctaacaa tccatacagg aaaaatcgtt tgttctcgga tactttctgt
    12721 acttcttttt gaacttcttt tgcatgataa ggaacccggt gccctctgac aagtaagcga
    12781 tgactgttga ccatataagg tgtacaagtt aggagtgtca cgaaatcctg ccctttttcg
    12841 attctcagat cttttgtatc agtcggttct accgtcttga tctgatcgac ttgataagcg
    12901 agtgtccgtc cattgatttc gatatagaaa gaatcaccta aagtcagttt cggtaagtct
    12961 gtgaacaact ttgcttgagg cagtcctcga tggctggaaa taacggcatg tgtattttct
    13021 ccccccgtag gataagacgt tccttccaat aatgaggcac ctttttctag cagcacagta
    13081 ttcgtttcat caaaaattgg caaatggaca ttgatctcag ggatcgtcaa aataccgatc
    13141 gtatgtttct caaaataggt tctatccttt ttcgcaggtt cttttttctt agtaaaggga
    13201 tctgcacctg gatttccacc ttcttttgct aattgctggt ttttctttgt catgttttct
    13261 tggattttag ccatgacttc ttcattttct ttgaccgctt gttgctgata atgagagatg
    13321 atctgctgat ccagataatt attcagcgcg tcgctaacaa atggataagc tagtatccct
    13381 attcctgaga gaagcaagaa aagcatcagc agatctagaa gtttcctttt ctttggtttc
    13441 atttgtgttc tccttgtgat gactgtttga taataaaaga aaaaggagca tttgcgtaat
    13501 ccgctgctcc tttttccaat tcttgcaaaa agactaaatc tgactgtgct tgcgtctagc
    13561 aaagtacagt ccagcaatca gtagaaggac tactcctgca ccgatataca catagattcc
    13621 cttaccgcct gttgaaggaa gtgtaccttt gtgtttattt ggtatttttt caggagaaat
    13681 caactgtcct gctgttacat aagattgttc atcgatagta aatgctacac ggtttgttaa
    13741 tggaacatat ttttctggcg ctttcgtttc ttccagatag tacgtgccat attttagacc
    13801 tgtcacatcg attaaaccgt tacttgtggt tgtaaaaacc gttgctgatt ctttcgccga
    13861 tacccagctg acggcttttg tggatgggtc gatcgataaa tatttcgctg tgtcactatc
    13921 ttgatcacga acgacgaatt ctgctccagc aagtgtttgg tctgatgtaa cgtcaccatc
    13981 tactttaaca aatcgtttgc ccccagtaac gacatcgact gacggtggtg tttgatcatt
    14041 tgtatgcccg ttatcgacat ttgcttggtt agaaaatcct ttggttggat ctgctttttc
    14101 gttcaaatgc atatagtaaa cgaatttcaa tgtaccgcca ggagttaatg aaggaatata
    14161 attcggatca actgaaaccg tgaatccgtc tgtttgctca gtgacagaat aatttactgg
    14221 gtcgatttct ttatttccat catataaggc ataagcatac gttcctgaag aatcattatc
    14281 aaatgttaaa gcagcgtcat gagtatcgat aagtttgaat gttgtgtact tgttttgcgt
    14341 tccttcttta tcagcgatac ctaatgggat attgacagaa atctcatatt gaattttttc
    14401 accgattgcg acatcttttc cattcaattg aggtgttgtt ttatcaactt tagacgtatc
    14461 atttttgatg gtcttttcta ctggtgtttg gctatttgcc aggatctcaa aaggcgtgat
    14521 tgtttgcttt tcaatcattt ccgcattatc aggagctttt acttcttcta aattatattt
    14581 tccaacttct aaatgattaa cgatcaactg gcctttttca atagatgcct cggcaaagtc
    14641 attgttgccg atgtcataag aatgaccagt aatgaaatgt ttggctttgg tttgatcagt
    14701 tgtccaagtg tacaatccat ctgtgacact ttggatgtat tttttgacgc ttggtgttcc
    14761 ttcttcttta gaaataataa attctgctcc atttagtgct tcgttttcgg cagtaccgat
    14821 ttttgtaact ttcaacgttc catcattacc gactgtattt ttagggtaga gatggatagt
    14881 atctagttct tctgtcccgt atttataaga gccatctgct tgtttgatca tctcatatac
    14941 agggaaagct aaaaccatgt ttgcggcagc tgacactccg tcttttggtt cttctttgat
    15001 cgtgtagact gcatctttcc cattttgttt tttaggtaaa gataaagtga catttccatc
    15061 agcatctgtc gttcctgaag caactggtgt accaggagtc aaagactgga ctgcttgttt
    15121 tgctgcatcc acggacgctc ctttatctcg ttgcgcataa aattcttgag tgacgttata
    15181 aactgaaaat gaaatatcgg ctaatccttg gtattgatcg aattcgctca tttctttccc
    15241 gctgttttgg attaaaggat cgggtaaatc agtcattttc tttttatgaa ggatgacttg
    15301 agctgctgtc tcctctgcaa acactttttt tgcgccgaag ctgtttgtga gtaatggtaa
    15361 aataaggaaa aggactccta acataacgtt tattttttta tgatttttca ttttttcact
    15421 cctgttcttt ttttgataaa caaccagatt gcaaagcttg cgaataccat accaacaacg
    15481 cctaaaagtg tatttttcgt ttcgtttgtt tttggaagaa atggacggct attttctttt
    15541 ttattgacta ctttcaatat actgtccgcc tctttatcct cagaaagaaa agttgaaatg
    15601 gtaaacggaa tttctgtttc acttaaaata taaccagaag gtgcttcgat ctcttttaat
    15661 cgataacttc ctgttttcaa cccggaaatt gaaaaagcgc catttttatc ggaaatcagg
    15721 tgaagcgcat ctgattcgtt ctttgtccac ttgtatccgt tggctgtttg ctggaggtaa
    15781 tttcctgctt tgtttttaat aaggaatact gcgccagcta atcgtttatc tggtttattt
    15841 gtctcgattt tgacaaattg cttactccct gtatatacag agactttctt tgtactgaca
    15901 ccaaaaccaa aatccagtgt tccgttgttg ttgatttcct tgttcgcctg tgctgtactg
    15961 cttagctgca tctgataaga aatggtcagt ttttttccag catatttttc caacgaaggg
    16021 atcgaaaata aaacatcaaa tccgtgaggt ttcttgtgta tgacaaagcc ttctgtcagc
    16081 ttttctccat tcgatgaaat cgttaaacta ttaggtaaaa acgttaatgc ttcatccgca
    16141 ctatctgaca attcgaactt ttgatagtca aggatattta caggaattgt cgtatgcaag
    16201 gaataagtga ttttttcacc aatcgtaaaa tcatttctag gctcttctaa taccttctca
    16261 aaaggtggat cagggtagtc gttttcttca tttttaggat acaaatggat agttgaaagt
    16321 ttttgattgt tttgtccatg tacaggaaga acaactacca tattctctgc cttttctttg
    16381 acgacttctg gtactttgga ttcaatgaaa agatacgctt tatctctttt ttcttgatct
    16441 ttagcggcca gagaaaaaga agcgatacca tcttcgttgt taagagttgt agttgttgct
    16501 tctgcggtaa acataccgga agacgcaccg ttttttgcga tctctgcttg tgcttcttct
    16561 accgttttgc ccttttcccg tagatggtaa aaagaatctg tgacatcata aacttggaat
    16621 gtgacaccat ttaatcctcg atacgtttgt aataaagctt tttcttgtcc gtcatttgga
    16681 tgatttttcg gcagttgccc gtttgggaaa agcaatttgt gtaaagtaat ctgaaccatt
    16741 tctgtttctg tttctgttgc tacctgagta aaagctggtt taaatagtaa caagaagaga
    16801 cacatactaa gccaaccaag ttttttcata tttagctgcc ccctctttct tttttcaaaa
    16861 aaagatacca aatagaaaac gcacaaccaa tcatccctac tagatagatt cctaaacggc
    16921 caattcctcc cgtttcaggt aatggtactt ttgcctgatt cgtgatgtct aaagaaatct
    16981 ggttgttttt ggctcctggt gacagaacag attcatgatc ttgtccatcc acttgaattg
    17041 acccatcttc gtgtataact atagtaactg gctcttttag accttggtat ccttctggtg
    17101 taaaagtttc ggtcagcgta taagttccag gttttaaatt ttcgaataga aaggtatctg
    17161 tttcttgtcc atcttttggc gattctaagt ccatttctgg tccctgcagt ctgaattttg
    17221 ctccttttaa tgtctgtccg ttgtcttctt ttttgtgtac cgttagatca aacgctttta
    17281 gtcgattttg atgagtgagc gtccatacat tatttgtcat ctcggtattt tttcccgtgt
    17341 aaatgatttt tccatattta tcgatttgga attcataatt tcccggcgaa gtatcatatc
    17401 cggcaggacc agctgtttca acgactcgat atttaccagg tgtgagtgaa tcaaaactgg
    17461 caagaccttt ttcattcgtt gtttggctgt caattggctg ataagtacca tttgcttctt
    17521 ttttctgtag ggaaaatgct gctcctttaa gatttatctc tttgtccgtc ccttgcatcg
    17581 catacttacg tactgctaca ggaacttcaa caaaaggatt ttcaatagtc aactggatcg
    17641 tatcatcgga agtagtgact gtttttccat caatggttac cgtaccatca gaagcgatct
    17701 tgatttccca agtagtcttt ttgcttaatc catgcccttc tggagctttt gtttctgtca
    17761 gcgtataggt catttctttt tgcaatttga cattttctgg aagagaatag gttccgtcgc
    17821 catgatctgt tagtaaagta tcaatagaat ctcctgttaa tttgaaaaca gcgccaataa
    17881 gatccttttc acctgtagag gaatttttcg ttattttcaa gtttaacggt gtgaattgtt
    17941 tagtattttt ccatgtcatt gcatctattt gttgagaatc gtatcctggt acaggtaatt
    18001 ctttgattgt ttggtaactg aatgcttgac cttgattatt gtattgaggt agtgataata
    18061 tctcttgata actttctccg ctatttgcag ataatttgtc aatgtctgca cgttcccacg
    18121 tatttgttgt atctttagct ggtttaatga ttcgaatata tccgtttttc caagctgcag
    18181 catttgtcgt atgttcccgt tgaatctcaa aagtaacttg atctggacga tcagctagat
    18241 tgttgtcaaa ttcttcccat aactttttga tgtgaagact gactccggga gctttagcag
    18301 atggtatacc gaattcagct aattcattgg tatcaatgtt tggctggaaa gttgtcctgc
    18361 cgttcatttg ataccagaaa tttggatgga agtcctcatt ttctgtttgg attctcactt
    18421 ggtaatggat ttggatttct tggtcttttc ctaaatagat ctgattgctc ttgatggtat
    18481 ttccttctat ggaaatagat ggagtgaccg ttgtaggact tgtccccaca ctcttgactg
    18541 aaagtgtacc aggctgataa atgaatggtt ctgcgattgg atcatttatt tgtccattgc
    18601 ttacagttgc tgagatctgt acagcttttt tggctaggta ttcagagata tctgttgcat
    18661 gatcagctga ttcatagtaa agatcgcctt tttcatctga tgaaaccatt tgtcgcatac
    18721 gagactctac ttctgctttt gagagaccag ctgccggatc gctttgaagt tggatgccaa
    18781 gaccatgtat ttcgattcct cgttctttga gtgccatcgc ttctccgatg gttgcgataa
    18841 acgtactgtc gattcttctg gatagattgt tttggtcagg tgcatcatag attcttgaga
    18901 ttagagaagt atttcccggc cgatcttgcg tattagaaaa ctgagttccg taataattgc
    18961 tgcttgattg tgcgtgtacc cgctgtactt tataggaaaa tgttggtaca ccatccgtca
    19021 gcaaaacgat cactttttta tgtccatttg gaacggatag catgcttcct gcatctctta
    19081 gtgctttttg tgtaaaagta ccaccatttg tccgtgaagg tgtaatggat tttacttgat
    19141 ttttcactga atcaaatgaa cccatctgta ctgcaccgtt actgtagcta tatccttcgc
    19201 ttgagtagcc gacatatccc atattgattt tgtctgtgat accgctatct gctaaagtat
    19261 cgacaaaacg atcgacacca atctttactt caccgatccg attattgtcg ttcatacttc
    19321 ctgaccagtc tacgaccaat acgagatcaa gaggcgtgat atctttttgt acatttccac
    19381 gagcattcaa ataaacatca aacaatccgg gtgtacttgt ttcctttgca tactttcgta
    19441 tcgcatagtc tgcttgatta ccggttcctc catattcgat atacgaatgg gtccgatcat
    19501 taggatctcc attccaagaa tttataccat cccattgatt tgttccgttt ttattgcctt
    19561 gatgatcaag aacatttgta tttcctgtag gttgccaact ggctttagga taagtgccct
    19621 gatcatctgt tgtatattct gtaggaatcg tttgattaga tggcacactc acagctggca
    19681 aggataatgt aggcaatgta aatgggctga cagaattttc gttttctttt gatgattctt
    19741 cttctatttc gtctggcttt ggtactgaca catctgtatc tttcttattt tctgtctcat
    19801 caataatgtc atattcttta ttatcgacga tcagcttgtt tgcctggctg tggtaaagtg
    19861 ctggatcgat tggaaaactc agatcgatag tttgtttagt gtttgatgag atttccaacg
    19921 taattttccc gctatttacc tgataagcat agccatctcc ttcttttctt tccacatcac
    19981 ttaagtaatt ttcgactaga ccaatttctt tttctatctt ttcagcactg gtgttattcg
    20041 catttagctt gatgttcagt gctttttttc cagactgatc cgaaatgact tcagctgaaa
    20101 ttgtttctaa aactgttgga tcatctgtag tgtctgctat tgcagataat ggcacaaagt
    20161 ttgataaact caatattatc agcatgaaaa taactttcag tttcttccct gtggtcgtca
    20221 tttttcccct cctttttctt tttttctgtt ttgacgtgaa tagtgaaaat tattaaaata
    20281 aacaggcgtt acataatttt atttatttta atatgataat tattatacga agggcgctaa
    20341 agggaatcac accaattttc ataccaattg gtttcattta tgaagttgat tccttcgatt
    20401 aatgagcgtt tcattgaaag aaaagggttg agggagaaaa acatatgatc aggttgttgg
    20461 aatgaaacta tagaaagatg gaaaaaatca ttcgaattct gtccatttct gacaagtgga
    20521 tgactaaaaa agaacttgcc aaacgaattg gtagttcaga atctacattt gtaaggtata
    20581 tcgaagagat caaaatccgc tggggaacgg atgtaacgat tcaatcttca caaaaattgg
    20641 ggtatcgtct tgaacatttg aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc
    20701 aaagttctac aaacagtcgt ttattaaatg agattattgt caatccgggt aaaacgattg
    20761 agtattattg tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat
    20821 gcaatcaaat tttagaaagc tatttattaa aagtgatcgt tgaccaaggt tttcagttaa
    20881 ctagccttaa aagtgagctc cagttgcgta tctttgttac ctttttcttt ttaagttact
    20941 acggaagacg cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa attatggtca
    21001 agaataattg tcagatgaat ttgtttcagg aagaaagccc atatgagcaa tctttcttta
    21061 ttatgtattt tgttgtgcat ctcatgagag aatcccaagg aactccattt gaatggaaag
    21121 ctggagactc ttatgtacag ttgataaata tcgataaaaa agacttctta atcttgaaaa
    21181 atctcttagg aaaattgaag tacagcagct atataaaagt agtgaactat tttgtcaata
    21241 cgattttttc agaattttcg gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa
    21301 atatgtccta ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa gaatatgttg
    21361 ttcaattatt aacgcaaatt tattttttct cccgtatttt cccatacgat acgactcatt
    21421 tgacgatccg tgcaacttac tttgcacagc agatgcaaca ccgccagata cgtctatatg
    21481 ctggatttcg tgcagaactt caattttatt ccactctact gaatcagaat ctgatttatt
    21541 atttatctag ctatatcttt tggatgctta tcggtaatcc tgaaattcac cattatactt
    21601 ctgataaaaa aatactgatt atcagcgact taagtcttcg acatagtcaa tatattgaag
    21661 aatatatcag tgatattctt gctgtgcata aaattcactc tgagacaact gctattactg
    21721 aagatcagct gtcgaaatac aatcttctcg agtatgatct gatcgttacg aatcaaccca
    21781 ttttgaatgc ccatgttcct catatattga ttgatgatag tgtgtcattc gcaaatgaag
    21841 aagaactcat ccgcttattc gagttatgaa taatcaaaaa acagctatgt ataaaccacc
    21901 ctgataaaaa tttcaggaca aatacacata gctgtttctt tttattgtat tccttcagca
    21961 atccaacgag atttatagcg aaagtctttt aacaactctt cttttgtcca attatttgca
    22021 atgagatccg tcaaagtgtt cattacgagg gtcatggaaa agtataggaa actaactgct
    22081 tcttccgacg attgaatgga gaaatgattg attcttaata attccagcca tttttcgttc
    22141 attggagaat cagccgatag ttctctacgt ttggttgtct gctgatcatt tcgctgggtt
    22201 aacaagtata acctttgcca atagatactt tcgtgatcta agtcgcagca ccactctaaa
    22261 tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt taagatatct
    22321 tgatggacta tgtgagaact gcttttgatc gtatacaaat aagcatcttc caaatcttga
    22381 aaatatttat aaaaagcacc gcgcgacatc cccatcttgt caacgatttc tgagactttg
    22441 acttggctga tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat tctctgtttc
    22501 tttgcgttcg ataaattgaa aaaagtttct tttggcatcc acatctctcc tttatctctg
    22561 attatacgtg gatagccctt cattgtcatg aaggattcat tccttctttt tctcttcttc
    22621 tgtcaagtac ccatcttcca ttcgataaac gcgatcactc cattgaatca tacggacatc
    22681 atgagtaacc ataatagtcg ctttttgttt ctcatgagct tctttaacta gtaatttgac
    22741 gacttcatat gcatgatctg tatccaaact agctgttggt tcgtctgcta agatcaagct
    22801 tggctcatta tataaagcgc gagcaattgc cactctctgt ctttctccac cagaaaggtc
    22861 tttaggataa ctatttttaa gtgactggat atctaaagat tgcagcaagg aatcgatttt
    22921 tgttgaatct tctttttttg aagtaacttg gtcaactagt tcaaattgtt cctttaccgt
    22981 taaaaaagga atcaaattag agctttggag aataaatcct atttctttaa agcgtaaatc
    23041 tgctcgtttt ttttctggaa gctcagttag tgagtgaccg ttgatcacta tttctccgga
    23101 agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta cttttacctg aaccagaagg
    23161 accaatgatg ctgacgaatt ctccttcctt tacataaaaa tcaattgctt tcaaagctga
    23221 gacctttgta tgaccagaac caaatgtttt ggtaactttt ttcatcgtta atattttttt
    23281 catttttatt aacctcctat tgctgtaatt gggtctactt tggttatcgt acgtatcgaa
    23341 aacaatccgc ctagcattgc gacaatgatc aaaatcccac tatatagcaa ccattgtgac
    23401 caaaaaacag caaaaggcat cgcgcttggt aaaatcagac tagtcaaata agcaaacaaa
    23461 agagcaagca gaacgcctac caccccaaca ataaaggact gtgctactaa agatttagcg
    23521 atgaagaaat ttttgatccc ttgtgctttc attactccaa aaatagcagt cttctgaagt
    23581 gtcatcacgt acatgaaaat accgaccacg gcagcaacca ctaagaacaa aaagtatatc
    23641 atcgcattta aggtcatatt ttgggcagaa tagccaggaa tactttcaat gaatgtccca
    23701 atagttaata actgtagacc cttcgatgct ttttctttcg aaattttagc tgaatccttt
    23761 attacgatcc cattgatcgg ctgttcttct tcagaagcaa acggctgttg tccatacttc
    23821 aaagcagtcc acgtatctag ggacgcatag atgacgggag aaactgtgta ataagtttca
    23881 ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc caattttgat cgtatctcca
    23941 attttatagc ctgaatcagc cagattttga gagatgatga tttcattctt tgttttaaat
    24001 tcatgccctt tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc gaaaactgta
    24061 acattttctt tgttttttcc tttgattgct ccgctgtaca atccgattgg tgctttctct
    24121 cctccttcaa tgtatttcag atctcctctt gttaattgtg aagctgcaaa tacttgattt
    24181 gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt tttaaactct
    24241 tctgaaagtc cattcgctag tcctgacaac atgaatacga catacgcaat caagaacata
    24301 ataccgacga tcaagccata tcgtaattta gaatagcgaa tttctttgat agctagaaac
    24361 atttgttttt cctccctatg atagattgat agtgacaaaa tgtcacttgt tgaatagtgt
    24421 aactctaaag tgacaatttg tcacttgtgt tgtttttttc ttatcaataa attgtctaag
    24481 acatctttcg ataaaacctt aacatgagtc ataaaaaatt tatttaagcc ttatttatgc
    24541 tttccatgat aaactaaagt gaccaaatca atgaggtggg aaaatgatta aactattagt
    24601 agtagaagac gataaagtat tatccaataa tataaaagaa atactcgctg caatcggcga
    24661 gataacacaa gtttatgatg gtagtgaagg actgttcgag ttggaaagtg gagtttatga
    24721 tcttgcaatc ttggatttga tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat
    24781 acggaaagaa aaaatacaga ctccagtatt gattttgact gcaaaagata gtttggaaga
    24841 taagattgaa ggatttgaac gaggtgcaga tgattatcta acaaaacctt tctatcgaga
    24901 agaactgatc atgcgcgtga aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa
    24961 tgttctgaag tataaggata tcagttgtga tttaaaaagc aatgaagtct tgtataaaga
    25021 acaagttttg cccatacaag gaaaagagtt tgaattgctc gtttattttt tacaaaataa
    25081 gggaatcatt ttgacaaaag agcagatatt tgaccggatc tggggatttg attctgatac
    25141 gacgattaca gttattgaag tttatatgag caacttacga aaacatctgc gaagtactgg
    25201 attggataaa gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg
    25261 caatgaatgg agatattaca cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag
    25321 catttgcttt tatctttttt cttttgggat tcattacttt acagatatta aaccaatctg
    25381 catacagaga aacagatctg tcattagaga aaataaattt tgattctcga acagtacaat
    25441 tagagataca aagatatcag caaggcgatc cctttttaaa cccgactcaa acggaacaaa
    25501 acagaacaaa catggaacag ccattaatgg aaccatcaaa tgaccgtttt aacactcaag
    25561 tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc agctattggt gggcgtttca
    25621 accaaatcaa agaattaaaa atggatacga aaaatctgaa taccatccaa aatattgaat
    25681 taaatgaaga cagtggctca tctgccctct ctttccgttc gattacgaaa aaagcggaga
    25741 cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt
    25801 cattagcgac ttttcgcacg attatgattt tgtgtatgat tgttttttgg attctctcta
    25861 ttggtatcag ttattacttg tctagtctaa gcatgcgccc tattattctt tcttggagaa
    25921 gacaaaaaga atttgttgag aatgcttctc atgaattgcg cacaccgtta acgattattc
    25981 aaaatagttt ggaacatctt tttaccaagc ctaatcatac tattattgaa gaatccgaaa
    26041 gcatcgctca agcattgtca gagacgaggc gcctcactgg attgacttcg gatttgctga
    26101 caatcgctcg gaatgattct gatcagcagc tgctttcaaa gcaaatgatc aatacacaag
    26161 aatatatcga acaacttgtt aaaccatttc aagagatggc catcatagat ggaaaagagt
    26221 ttattctcca taacgatgca acattagaag taccaattga tgaaaaaaaa atccatcaag
    26281 ttttggttat tttgctggat aatgcattaa aatatacgaa tccagaagat aagatcattt
    26341 tggattcatc tgttaaccat aaagaatggc tgattcacgt aaagaatact ggcccttcta
    26401 tcagtgaaga agatcaagca aggatttttg atcgatttta tcgggaagat caatctcggg
    26461 cgaaagagac tggcggatac gggcttggct tggcaattgc aaaacaggta atcgaacagc
    26521 atcatggaca gatttctgta tccaattatc ttccacaagg agtcgatttt aaagtccaat
    26581 tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac aaaaatcttg
    26641 tcacagccct tttttgttgc gaataaacgg tgttccagaa gtagcttctt cggaaataag
    26701 ccgaaatttc tttaagctga ccacttctgt cacaacctct ttagcttacg cttacgattt
    26761 tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca atatccaatt cttctcggct
    26821 aacatcttct gtcacttttt caacatcttc atgttcttct ttatgaatcc caacttcttc
    26881 acgaataact gtatgtttgc ttacatctac ttgctcttct ttgattggga tagtaatcgt
    26941 gtcatcttcc aatttttcgt ctgttccctg tgaagattga tctgttactg gtttgcgttc
    27001 gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt tgaccacatg
    27061 tttgtggatg tcgacttctc cagttgtcac atcatgtgta tttacatcta atttttcttc
    27121 ttttagttga attgtatcat catctagatt tggtttgcga tcgatgtctt cagtcttgct
    27181 tgtacccatt gtatcagtct cgccaggtac gtccatcgga tcagttcctg gtgtcatatt
    27241 caatggatcc gttgtatttt gaaagcctgt cgtgccacct acgtccattg gatctgttcc
    27301 agtgccagtt tctaatccag tccccattgt gcttgctggg ctaacagtat cttgtgtatc
    27361 cattgtatct tctgttgttt ctggctgacg atagcctttt actgtgatga caaattttcc
    27421 atcagaaata tctcctctat attgatataa aggatcattt tctggtgtag ccgtctcaga
    27481 atcataggta tcaaaagaaa aagtatcttt tattttttcc cataaagaac ggtcttcttc
    27541 accatccatc cgattttcat ctgtttcaac atctatacca gacaatccgt caaatcctag
    27601 tctagatgct gcttcatcag tagtataaat aacgatatca tctcgttcat acccttcgtt
    27661 acgcaaccgt tcaattactg ataatgcttc ttccctcgta ctatagcttc cgatgaccgt
    27721 taaatttttc tcttccatgt aaattcctcc tagcaaatag atttatgatt ggta
//
LOCUS       DAZUMV010000022        27423 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000022 DAZUMV010000000
VERSION     DAZUMV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.022"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..416)
                     /locus_tag="ACG8FK_000886"
     CDS             complement(54..416)
                     /locus_tag="ACG8FK_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096515.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(513..869)
                     /locus_tag="ACG8FK_000887"
     CDS             complement(513..869)
                     /locus_tag="ACG8FK_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS5096516.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSD"
     gene            951..2126
                     /locus_tag="ACG8FK_000888"
     CDS             951..2126
                     /locus_tag="ACG8FK_000888"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS5096517.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2203..2787)
                     /locus_tag="ACG8FK_000889"
     CDS             complement(2203..2787)
                     /locus_tag="ACG8FK_000889"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5096518.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            2988..3986
                     /locus_tag="ACG8FK_000890"
     CDS             2988..3986
                     /locus_tag="ACG8FK_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5096519.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4098..4607
                     /locus_tag="ACG8FK_000891"
     CDS             4098..4607
                     /locus_tag="ACG8FK_000891"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS5096520.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4637..5296)
                     /locus_tag="ACG8FK_000892"
     CDS             complement(4637..5296)
                     /locus_tag="ACG8FK_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS5096521.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5298..6062)
                     /locus_tag="ACG8FK_000893"
     CDS             complement(5298..6062)
                     /locus_tag="ACG8FK_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS5096522.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6070..6747)
                     /locus_tag="ACG8FK_000894"
     CDS             complement(6070..6747)
                     /locus_tag="ACG8FK_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS5096523.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6784..8169)
                     /locus_tag="ACG8FK_000895"
     CDS             complement(6784..8169)
                     /locus_tag="ACG8FK_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS5096524.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8379..8463)
                     /gene="ffs"
                     /locus_tag="ACG8FK_000896"
     ncRNA           complement(8379..8463)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8FK_000896"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8486..9001)
                     /gene="tadA"
                     /locus_tag="ACG8FK_000897"
     CDS             complement(8486..9001)
                     /gene="tadA"
                     /locus_tag="ACG8FK_000897"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS5096525.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9108..9965
                     /locus_tag="ACG8FK_000898"
     CDS             9108..9965
                     /locus_tag="ACG8FK_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5096526.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10046..10381)
                     /locus_tag="ACG8FK_000899"
     CDS             complement(10046..10381)
                     /locus_tag="ACG8FK_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096527.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10448..11818)
                     /locus_tag="ACG8FK_000900"
     CDS             complement(10448..11818)
                     /locus_tag="ACG8FK_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS5096528.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11896..12432)
                     /locus_tag="ACG8FK_000901"
     CDS             complement(11896..12432)
                     /locus_tag="ACG8FK_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5096529.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12741..14222
                     /locus_tag="ACG8FK_000902"
     CDS             12741..14222
                     /locus_tag="ACG8FK_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5096530.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPICFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14325..15032)
                     /locus_tag="ACG8FK_000903"
     CDS             complement(14325..15032)
                     /locus_tag="ACG8FK_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS5096531.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15111..15449)
                     /locus_tag="ACG8FK_000904"
     CDS             complement(15111..15449)
                     /locus_tag="ACG8FK_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS5096532.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15560..16522)
                     /locus_tag="ACG8FK_000905"
     CDS             complement(15560..16522)
                     /locus_tag="ACG8FK_000905"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS5096533.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16537..17868)
                     /locus_tag="ACG8FK_000906"
     CDS             complement(16537..17868)
                     /locus_tag="ACG8FK_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS5096534.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(17876..18556)
                     /locus_tag="ACG8FK_000907"
     CDS             complement(17876..18556)
                     /locus_tag="ACG8FK_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS5096535.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(18775..19254)
                     /locus_tag="ACG8FK_000908"
     CDS             complement(18775..19254)
                     /locus_tag="ACG8FK_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS5096536.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19381..21060)
                     /locus_tag="ACG8FK_000909"
     CDS             complement(19381..21060)
                     /locus_tag="ACG8FK_000909"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5096537.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21608..23110
                     /locus_tag="ACG8FK_000910"
     CDS             21608..23110
                     /locus_tag="ACG8FK_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096538.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23123..23785
                     /locus_tag="ACG8FK_000911"
     CDS             23123..23785
                     /locus_tag="ACG8FK_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096539.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(23886..24089)
                     /locus_tag="ACG8FK_000912"
     CDS             complement(23886..24089)
                     /locus_tag="ACG8FK_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5096540.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24200..25384)
                     /gene="iadA"
                     /locus_tag="ACG8FK_000913"
     CDS             complement(24200..25384)
                     /gene="iadA"
                     /locus_tag="ACG8FK_000913"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS5096541.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25541..27279)
                     /gene="yfcC"
                     /locus_tag="ACG8FK_000914"
                     /pseudo
     CDS             complement(25541..27279)
                     /gene="yfcC"
                     /locus_tag="ACG8FK_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
BASE COUNT         8150 a         5736 c         4652 g         8885 t
ORIGIN      
        1 aaaaaaagaa gtttgaaaaa aattcaaact tctttgttcg cttaatctgt attttagaag
       61 aaaggcactc cctctacttc gtaagttcca agaacagagc gcatttgacc tggttcttcc
      121 caaatatatc cttcgcctaa ttcttctctt tgattctttt gttcagtttc aaagacaaac
      181 aacggaacat tttcctcttt aatatgtgta tttgtcaatt ccatcaaact aatcttgcta
      241 acatcgatat gaactatttc ttttaagaaa ttaagtatat tcgctaaacc agtctttcct
      301 tcagaaagtt ccgctaacgc aaattccaac ttgtcatcaa ttgaatggac caaaaatttt
      361 ttagtaccat cctccaaatg caacataatt gtaccagcaa ctcttttgct tgccaaaatt
      421 tccacctcga tcaacttgtt ctatggctta cataaaaatt ctagcacatt ttcagaaaaa
      481 atacactttt tatttcttat tttctttccg ttctagtcag ataatgcttc ttctatccaa
      541 ataggtaact gctccgcaat agtggtaaat ccgatcttac gatttttatt cgtgaaatac
      601 ctttttctgc gatatggaac agctggattt gttttttcta acgttcttaa agataaactg
      661 atttttttgg aatattcatc aatgtcgatg atctgaactg ttactggctg tccgactttt
      721 aacaatgaat ggatattttt cgtataaccg gattgtactt ctgaaacatg aataagtcct
      781 tgcgtctcgt tatcaagaga aacgaatgcg ccataaggct gtatccctgt cacttttcct
      841 tcaattattt gtccaatttt ataagtcatc tacttccctc tcttcgcatt tatcattata
      901 ctgtaaatag ctcaaaaaaa gaagcgacga aaatgactgg aggtcttaag atgatcgaat
      961 ttgataaaca gatttcaaga aaagatacag aaagtgtaaa atgggacgca attgctgaga
     1021 catatcaaat ggatgactta cttcctttgt gggtagcaga tatggacttc ttgtctcccg
     1081 gtggggtagc taaagcgttt agtgaataca tcaaacatgg aatctttggt tatgccactc
     1141 tgtctgataa actatatcag gctatcattc attgggaacg ccaacaccat gctgtggagt
     1201 tgacgaaaga aaatatcgtt tttacaagtg gtgtgttaac cagtttagct gcagcagtac
     1261 aaacttttac aaaaccaggc gattctattt tgattcatga cccagtttat cctccatttt
     1321 cctctattat agaaacaaat caacgtcaca tcgttcgaag ctctttgcta gaaaaaaata
     1381 atcatttcat catggacttg tctgacatgg agaaaaaaat cgaagaaaac aatgtaaaag
     1441 caatgatttt atgcaatcct cacaatcctg gagggcgtgt ttggagcaaa gaagaattga
     1501 aacagttaag cgaattgtgt ttaaaacatc aagtgattct gtttagtgac gagatccatc
     1561 aagatttaac tttattcgat catacattta cttccatgct aacgattgat cctaatttag
     1621 atgatctact agttgctttt acttccgcaa ccaagacttt caatctagct gcaatcaaaa
     1681 attcaatggt atttatcaaa aatccagaga tgaaagctga ttttgaaaaa catttagtga
     1741 tgaaccaaca gcacgaaatc aacacttttg gactgattgg aacccaagcg gcttatgaga
     1801 cgggtggaga atggttaagc caactgattc cttatctaga aaaaaatgtt caaacagtta
     1861 atcagttttt tgaacagtat cttccgaaag tgagaataat gaaccctgaa ggaacttact
     1921 taatgtggtt agattttacc gcctatattt cagacgacca agaattggag aacactcttg
     1981 ttcggaaagg aaaagtcgta ttaaatcctg gcatcacgtt tggtccttcc ggtcatgggc
     2041 atatgcgctt gaatattgct tgtccagaag aaactttact ggaaggactt aatcgaatca
     2101 agtatgcttt agagacaatc gattaaatca tttattcaaa ataaaaggac gagaccttac
     2161 taggtttcgt cctttttcat aattactccg taccatacca aactattctg aaatttcaat
     2221 tgtttcaatc actacatcat gtacaggtcg gtcttgtgga ccacgttgta catttgcgat
     2281 ttcatctact gtgtccatcc cttctagtac atgtccaaag actgtatgtc ggaaatctag
     2341 ccaaggagtt ccgccacctt tatacgcttc aatgatttct gatgggaatc cagcaccttc
     2401 tagttgtcca agcatattag ctggcacatt tgtgttattt acaataaaga attggcttcc
     2461 gtttgtgttt ggacctgaat ttgccataga taaagctccg cgaaggttga acagttcacg
     2521 actgaattca tcttcaaaac tttcgccata aatactttcg ccacccattc ctgttccagt
     2581 tggatcgccg ccttggatca tgaaatcagg aataacgcgg tgaaaaatca cgccatcata
     2641 ataacctttt ttggaaagtt caatgaaatt ttgaaccgtc ttcggtgcta gttcagggaa
     2701 taattgaaca gtaatgtcac cacggtttgt tttgataaca gcttttggtc ctttttcact
     2761 ttttaaatct agttgtggga atgccatatt gtttcctcct aatatttgca tacttcataa
     2821 taataataac ataataccta acctctcgta aaaatactag atttgttttc ttttattttt
     2881 catcactaga tacattctat aacttaccgt ttcgatttcg aattcaaaca tttttgagaa
     2941 gcattctcaa ttaaattctg ctatagtgtg aaaagggagg aatttttatg gaaatctatg
     3001 acattacaat catcggtgca ggtcctgtag ggatgtttgc cgctttttac gcaggaatgc
     3061 gccaagctaa aacaaaaatc attgacagtc tacctcaact tggtggtcaa ttagcaactt
     3121 tgtatcccga aaaatatatt tacgatattc ctgggtatcc agcaataaaa gcaagtgaat
     3181 taatcgatca attagaaaaa caattaacta cttttaacca tacattccat ttaaaagaag
     3241 aagttctctc gcttactcgt gaagacgaag ttatcgagat tactacaaat aaaggcatcc
     3301 attattccaa agcagtcatt ttagctttag gaaatggttc attccagcca agaaaattga
     3361 atttagataa tgctgaatca tttgaaaacc acggattgga ttatttcgtg aatgatttga
     3421 tgaagtatgc tggaaaaaaa gtcgctattg ctggaggtgg tgattctgct atcgattgga
     3481 cgttgatgtt agagcctatt gcaagcgaag tttacttgat tcatcgtcgc ccggaatttc
     3541 gtgcgcacga acatagtgta tcacgcttga aatcttcatc tgtcaacttg cttactccct
     3601 atctcatcga tggattatca ggcaataatg gggaactgac agatatccgg ttaaaaaaag
     3661 tgaaaagtga cgagacgatt gatttgatga ttgattctct gatcgtcaat tatggattct
     3721 cttctaattt agaacatctt tcttcttggg gacttgatag taccagaaat gccattactg
     3781 taaaatcaga tatgtctact tctattccag gcgtttatgc agctggtgac atctgtacct
     3841 atgaaggaaa agtgaaactg attgctactg ggctcggtga agcacctact gcagtgaaca
     3901 acgctttaca ctatattaat ccaaaggaac gaactcagcc tggtcacagc acaagcttat
     3961 acgataaaaa tcttcgtcct agttgatttt ttatatctct ttgcatttca agttaaaccc
     4021 gaaagcagtt tcttgcattt ttttcatgtt ccgttaacat gaaatagaga aagaaaaaat
     4081 aaagggggag caataatatg gtcgtcaaaa ctgagacttt agaagaaaag gcaagagaac
     4141 tattgaagga acgtggcgta actgtagaag atatcgcaca acttgtcatg tttttacaaa
     4201 aagactatat ttctgatttg actttggcgg attgtactga aagtgtcaac agcgtgctaa
     4261 caaagcgtga agtacataac gccattatca ctggaatcca attagatata ttagcagaaa
     4321 agaaaaaatt gatgagtccg ttgcaacata ttatcgaaga tgacgaaggc ctttacggta
     4381 tcgatgagat cctagccttg tcaatcgtga atgtttacgg tacgatcggc tttacgaatt
     4441 atggttatat cgacaaggtg aaacctggta ttttaaaaga actaaacagc cacgatggcg
     4501 ttcatgtcca taccttctta gatgatatcg tcggagcgat cgccgctgct gctgccagtc
     4561 gattagcaca caaagaacca gaaaaaagaa gtcgccttac tcaataattc aacaaaaaaa
     4621 gaaagaaaac gtctatttag acgagttctt tcttttttgt ttgtttcaag ctattttttc
     4681 ctaagaaaag aaaaacagca aaaaataatt ctaataatac aaaaaataag ataaagctat
     4741 gcatgaaaaa ttcttctgga agtacattga tgattggatc agtaacggga tcgaataacc
     4801 agtcatcgtt gttgaaaaac gtttcatgaa aaaggataaa aaagcgatca aatcctatca
     4861 tcatgaaaaa accgaaaaca actggcagta acatgccgat ttgaaacgga cgaatcaacc
     4921 gccaaagccg atcattcttt tgcaaatatt tgataaacaa aatactcggg atcaaagtga
     4981 tgaaaaacac agcataatcc actaaaaaca aaattttcac ttcataaaag tgatgggctc
     5041 cgctagcaga aactgggaaa tctggcaagc tgaggatggt tttgaaggga ttattgagat
     5101 aattcattag gtgatcaaaa tttttcagta acgtctcttg atctaaagaa gtataatcca
     5161 aaatttgcaa atgtccgata tcaaatacgt atagtggtcg aaagttgatc gtcacaccaa
     5221 ctgccagact gatcagtgtc agaaataggc tgaacaatcc agcatattcc atccactgcc
     5281 atctcgaatt tttcatatta gaagctccat tcatccagtg attgtttcac ataggtagct
     5341 gggataggca acccaggtac atcttcttct tttgtaaaac ctgataaaac gagcaaagta
     5401 tctatattat tgcgtatccc agcttggata tctgtttcat agttatctcc aaccatgatt
     5461 acttcttctt tttgcagccc taatacttca acggctttat ccataatgat ggcttctggt
     5521 tttccgatgt agatcggcgg cgtttgggtt gctgtctcta caaaagagat gactgaccct
     5581 gctccaggaa gaaggccgcg ttcagtaggt atattcttat ctgggttcgt tccaatgaaa
     5641 gtagcacctt tttgaatagc aagtgttgct ttaactactt tttcataggt cagataatta
     5701 tctaagccta ccactacata gtcaggagcc tcttcttccc atgtaaatcc ggcagctaaa
     5761 atcaagtcaa tcaatcctgc ttctccgata acatagactt ttttcccttt tccatctgct
     5821 ttcataaaat cgattgttgc caaagtagct gtgtagactg tctctggagc tacatgaatg
     5881 tcaaactcat ctgctagacg attagctact gtttctggtg acttagtcgt gttattggta
     5941 acaaacaaga aaggtaattt tcttttttgc aattcttcca caaatcgttt accggccggt
     6001 atgggttgtg tcccacgata aatcgttccg tctaaatcaa ttaaatatcc tttatagtcc
     6061 attcatccac tactcttccc tttttctgat cgtaaatgct gtttttgttt tttctacttg
     6121 tttaggttgt tcttgttttt ttactttttc agaggttttt gctgactctg tttttcgatt
     6181 tttaataacc ggttttttgc gattagatgc gactggtcct tttttctcat cgatatacgc
     6241 acgattatgg ttttttttct tttttcggcg ctgagttgtc ttttcttttt taccgccgat
     6301 tctctcgatg acaaaatacg cacaaccaaa attgcaatat tcgtaaagat agtcttccaa
     6361 ggaatcgatt cgctgatctg gatgcgcccg tcgattatcc gcttggaaaa atccttttag
     6421 gcgtagttgt tcataacccc aatctcctac gatataatca tatcgggcta gtacatcact
     6481 gtaacgttct ccaagctttt ctgcattaaa tccttcacga taatcggaca caagccgata
     6541 atgtctttca cctatcaaaa aattactgtc atctaacagt gtcactagct cgcctttgac
     6601 tttttctgct ttaggctcct caacgatttc ttccaaagca gctgtcagtt cttcggtgac
     6661 tgttgtttcc tgttcagctg atgatttttt cttagcaatt tctgttttat gctcaacgtt
     6721 ctgctgttca ttttgttttg cagacatgcg ttcaccacct atctttttta ttataccttg
     6781 tttttatttg attgggtagt gagctttcaa gtaatctcct accacacttc ggataaattc
     6841 agggaacaaa aattcatttt ttccagcaat ctcaatcgtt ggaaaaaatg gaacaaacat
     6901 caaatgatct acggtagtaa aagtataatt tttattcaga tcgattggct tgttcttcca
     6961 aaagacttga tggttaatag aatcatatgt gattccatta tagaccatct gcccaaaaat
     7021 cttaccgcga aatcccattc ccttcatagg atagttacgt aaaaagttac gatttttttc
     7081 tatttctaaa actaagcgga tcagatcatt tccacccaat gtgacattta acaagtgcat
     7141 cggatgcggc aacgcagtgt gaagctgatc ctgattgacc cgcccttttg gaatttcagt
     7201 taaaaacaga ccactgttta atattgctgc ttccgtcttt cctcgttctt tcaccgcctc
     7261 aaaggctact tggataaacg aatgctcatt gaagatatct agactcagag aaaatggcaa
     7321 atctgctact tctttttctt ttaataattc atgcccacgt tctagataac tatttatttc
     7381 ttgttcatct tctgtaaaag ctgtcattgt ttcggtaggg atggctttgg ctgaatgctt
     7441 aaggattttt ttctcttcat cgatggttaa atggacttca ccgacatact gcccgtactt
     7501 tccagcggct gctatttgga catcattgat tacgtgacct tctctgaata aatgatgggt
     7561 gtgcgatcct aaaataacat caattgctgg tatttctttt gcaatttgga aatcatcctg
     7621 aatcccgaga tgactcatca aaactaaaac atccacttgt gaacgtaaat cttcgactaa
     7681 ttctggcaaa atatcataag gattccgtat gtcccagcca ttcggattat aagtaagcgg
     7741 aaatggagca gtaaatgcaa ttagtccgat tttcgtctgg tgtttcgttg taatgatttt
     7801 atatggtttc gtccatgccg gtggctggag cgtattttta tcgaataagt tatccaggat
     7861 gatatcaaac tcagcatgat cgtataaatg attcaactct tctttggaat ttcctacgcc
     7921 ttcattgttt cctatcgtcg ctgcatcata accaacttca ttcatcagca agatattttc
     7981 ctgtccattc gttgcttctg acaatggatg ccaacgatcc acaaaatcac ctagatctac
     8041 tgcaatcgtt gtcgtttttt ctttcttttc gttttctcgt ttacgttgat ccaaaaacct
     8101 tctgatcctt ggccaatttt ctaaatgaga atgcagatca ttcgtatgta aaatcactat
     8161 tttttccatg taccctctcg cctctcttta tctttatcat accataggca gctttttctt
     8221 ccattgaaac aagctctatt tcagagtttt tcctgccttg aagcaaattg tataagtaca
     8281 atattcgata aatcagtctg gctaaaaaat caggaacatt catttttaca gtactataaa
     8341 aataaaaaaa acagacccga aagtctgttg tcgtttcatt ataatcagac acatggcgga
     8401 acctgcttag tgctgcttcc ttccagacct gacacgcttc acaagcccac cattgtccta
     8461 gcctttcggc aaagaatagt ataccttatt tttcattcgt tgaccagtct ttttttaatt
     8521 tcttttttcg ctggcggagc tttcggaaaa agtcagtcaa aatcatccca cattcgtctt
     8581 ctaaaatgcc accttctaca tatgcttggt ggttaaaccg ctcgtcctcc aaaagattca
     8641 tcaaggtacc ggctgttccg ccttttggat catacgcgcc gaaataaact tctgctacac
     8701 gagacaaaat catcgcgccg ctgcacatag gacaaggctc taaggtaaca aaaagtgcag
     8761 catcttccaa gcgccagctt cccacttttt cacaggcttc cttgattgca agcatttctg
     8821 catgtgtagt agcatcttgc gtggtttctc taaggttata tcctcttcca atcacttttc
     8881 catccaatac aaccactgct ccaatcggaa cttcatgaag catttctgct ttctttgctt
     8941 ctgccaatgc aaggcgcatc catttttctt tttctactgc atcaagattt gtatttacca
     9001 attacccttc cctgcctttg ttttttcttg ttttacttta gcatatgata aaaaggctgt
     9061 aactaacttt tttttctatg gtaaaataaa tacaagaggt gaacaagatg aaaatggata
     9121 aattacttgc tctttaccca caagcacaga aaaaaaactc tccagcatca gaagcggata
     9181 tcctttcttt tgcaattgat gatgctttta tctggatcaa acaagattca ttatctcaac
     9241 aagaaaccag ccttttgaaa gcgttattcc ctgtaatcaa cgatcaaaaa aaacacccat
     9301 ggtatcacta tctttttaaa gatgcttctt gtgttcaaga aaaaagcttt cgtttgatcc
     9361 agcttcacgt cgaatcaaaa ggagaattct taaaaaaaga atggcaagat actattttgg
     9421 aattatttcg ctattctgct gatttctttt tttatacaga cacagatgcg ctcttagtcg
     9481 aacaaataaa ccgtgattat cttgatgcta ctgaaatcaa cggtattttt ctatctttag
     9541 atgctgattt tgatacgaca acgtctgtat tcgttggttc tttccattca cctggcaccg
     9601 acctcgtccc tttgtttgct gaagaaagaa aaattttttt aacagagcaa aacaatcttt
     9661 atactcacag ccgtactttt tctatgcaag atgtggcttt acattactat acaaaagata
     9721 ctgtaaaaga tagtaagttg atcaaaagtt acatagaact gatcaaacaa aatgaaatgg
     9781 aagtgatcat tcaagaatta tggaatgaat taggcaatgt cagctctgct gcaaaaacac
     9841 tgtttcttca tcgtaatact ctaaaatatc gaatcgaaaa atttcaagaa cagtctggtt
     9901 tcaatctaaa aaaagccaac gatttactgt tttgctatct tttattgctt catgaacata
     9961 attaaagata gattaaaggg gttgtgacaa taattttgtc acaacccctt atttcgaatg
    10021 aatagtgttc aaaagctagt ttctcttaca tcattttttc aacaaaatgc attagccggt
    10081 cttttacatc ttcggttgaa tctttgattg aatcaccata aacagagatt ttcttttttc
    10141 ctgatttgat ttttgctaac attcccatca aagaatccaa gtcactatct gaaagaccat
    10201 ctacgatttc tagtaatttt tcatttccat caaatttgtc atagacgaat ttttttactt
    10261 tcgtccgagt agtcatatga tggatctttt ccattacttt tcccgataca acgactgctg
    10321 tcgctacact ggcagcggct gctacgctta atccgattgt tacttttgtt gatgttttca
    10381 taagtatcag ctccttttta tctatctcca tcatagcata gacaaaaaaa taaacgaact
    10441 aatccactca gtgaaatctc aatcctttag ggaaaaagtt ctttactgtt ccttttactt
    10501 gtttcccaaa tccgacaggt atcttatcta atactagcaa aacccatcct tgattcccat
    10561 cacgttgaaa tgtttctcct gctgtgtaag attgccattc cttttgagtg atcggcaagc
    10621 aaggtatatt ctctatcttt tttgtagcaa gcgctaatgc atagctaggt tcaaatcgat
    10681 tctttttgaa atctcctaaa tgcaaacctg tacgtacgac ttttaaacca tcgagagaag
    10741 gggctaactc aggaacttcc caaagatggt cgttgaacac taaaagtctt ccagtagctt
    10801 cataatgaaa atcatttgaa aactccgtcc agagtttttc ctgctctttt gtcatttgca
    10861 ctttactttt ttttcgtgtc ttcttttctt tatgcatctg attttgtcca tgaaaagtca
    10921 gctttgcaac aaaatgtcct tcaccctgat ctttatgagg ccatatacgt attgtttttt
    10981 ccaaaccagc tacgcttccc cattcagaac gtcctgaact aacggattga gtcaaaggaa
    11041 tctcctcaat cgttactgga taattttcta caagccagct aatgatctct tcattttctt
    11101 ctggtgcaaa cgtacaagtc gaataaatca gttgtccttt attttttagc atttttattg
    11161 ctgaagacaa aatctcttgc tgacgttttt gacagtatag cggagattct tctgtccact
    11221 cttttattgc atttggatct tttcggaaca ttccttctcc tgaacaagga gcatcaacca
    11281 cgatccgatc aaaaaagccc gaaaaatgag ggacaagttc tgcgggtgca tgatttgtca
    11341 ctattgcgtt cgaaactccc catcgttcta tattttctga taaaatcttc gcacgtttcg
    11401 gaaagatctc attcgtcact aataaccctt tccccttcat ttgagcagct aattgggttg
    11461 atttaccacc gggagccgca cacaaatcca aaactttttc accgggtttt gctgcagcag
    11521 ctgttccaac gatcattgca cttggttctt ggctatattc atacccagct tgatggagga
    11581 aagactttcc attgacagtg ccaagaaatc cctcattgct gtagggagcg ggttgtaatt
    11641 cttcgctatg gtaagtttgg accatgtcca atcctgccgg ttttagtgga ttccaccgaa
    11701 atcctttttt cacgcttcct tgttcaagcg cagaaaaaaa atcggatgct tcttcgccta
    11761 aaagaagacg atattttttg ataaattgct gtggtaacgt tgtcgcttcc tccttcattc
    11821 caatgtttcg ttagacattt tacaatcttt tttacgaaaa ataaaggtgg ctgatctact
    11881 ttttcatgtt gctgcctaag gtgactgttc ctttaagata ttttccaagt attcatccgt
    11941 cacctcgttt ttatcccaaa cagcgttcat catttcaaga aaatcttgag aagaatcatc
    12001 tgagcgcaaa cttcccaatg cattaagcca taccaattca agcgactttt cttctttgat
    12061 cttcgtgtga cctttgacat atgagaaaaa tggccgctta aatagcgaat ccatgtctaa
    12121 atcagatttt gcaatgattt cacgaatact ttcatggata gaaaaaaaat ctttcaataa
    12181 gtagacaatg cttaaaacat catcggaact gcattcagct aagtagtttt caaaataaac
    12241 agtgatttct tctaatatct tctcttttaa cccgctgatt gatttgaaat gtgtataaat
    12301 cgggtatgta gaacaaccgc agaactctgc taactctctt gcagtaattg acttcattcc
    12361 atattcttcc acgtatacca tacttttgaa cagcaaatat tctttactat aatttgttac
    12421 tctccccatc attcttacct ccgtctcttt tcatctaccc ataaataaat aacataaata
    12481 attctaaaaa tattattgat aattatcaaa acatatttag ttatatccat taaaaatcaa
    12541 tttttccgcc ttaaaaacaa gaatatcttg acaaaatacc ctaggaaata aacaatagaa
    12601 taaacaaggt aacaaaaaaa ttaaataacg ttatataaaa aagaaggcga cactctgtga
    12661 gaataacaca gactgaaatg actttccact cacagttttt gtctaactat atacaaaaac
    12721 agaataaaaa ggaggtattt atgctaattg aagcaagaaa aaaagagtta cttgttctac
    12781 aaatggtcat tcaaaaaaat aattgttctt tacaagaatt atccaatgac ttacatattc
    12841 caaaacggac aatcaaagaa ctgatccgaa aaataaatgt atcactcaag caatttttat
    12901 tgattgatga atttatttat tctaatcata aaggagagat ccaaaccaaa gaaaccgatc
    12961 aaactaaaaa gttagctgtt ttttctaagt taaaattatg ctacctaaaa gaatccaatc
    13021 gttttaacta tttgatgtta ctaatcaatt atccacaaac tgctgtacca aaaaaatatt
    13081 tacttgaaca actttatata tcgccctcct atctcgaaaa actgacaaaa cagctcaatc
    13141 attccttaaa aaaattccat ttacggatcc tatctgtaaa aaattgctgt actcttgaag
    13201 gaaatgagtt atctatccgt ctctatcttt acttttttct ttcagataca tttggaggta
    13261 acgattggcc attttcctca cttgaattaa atgcactaaa aatgaacagt acagatgagc
    13321 aaatagaaaa aatttcaaaa caagaaaaaa atatctactt gctgttaact ttattcatat
    13381 tacgaaacaa acaacaggca tattttccaa aaacttcttc tgaaatcact gaattgatgg
    13441 acttattgca gcatacacat gattattcag aatactttac ttctttttca gtgagtcgcc
    13501 cttgtgaaga gacagaaaca gagagagcct actttaatta ttttttctat tgtttttttc
    13561 caaatcaaat ctcttttcga caaaaacagg agattggttt tttattttcc acaagcaaaa
    13621 accttatttg tcgacaagtc ggtcaattga tgacttactt aactcaacag tttcctctca
    13681 accatttaga aaataaaata ccaatttgtt tttattttct tgtgttggca gttgtttttt
    13741 tcgaactgtt gggtgaatcc agcagtagtt tttctacact ttgctttcca gaagccacgt
    13801 ccaatcattc tagttctttt atacaggaaa agaaattaag agaaaaaatc gatacaattt
    13861 tttcaaaaaa tggggaaaga aaccaaatca gtcaatacct tagtcaattg atccaacact
    13921 tactacaaac agaaacgaaa gagcagttac tcatccattt gcagataacc aaacagttta
    13981 caggaaatta tcatcttcaa agccatttgc agcagttatt tcgcaaagat gctgttaaaa
    14041 ttaccgcata ttcagaacat gctgatttga tcattacaga tcagcatgag aaaatttcag
    14101 atgaaacgga gactttctat ctggatacaa cgcacaatac agtgttattt ggcaagttaa
    14161 taaaagcggt tcatgaactg tatatgaaaa aaggttatga gaacaaaaca aagaggatgt
    14221 gacaaaattc ttgtcacatc ctcttattcc aaataaatgg tattcaatca gctaactctc
    14281 ggtattagct cagatcaaag cgctgatttc acaacttctt tattttaatc tcttcggttg
    14341 ccgccgaaca ataagatcaa gcgcaacagc tgcaggaatg tcgaaagcgc tgctgcaaca
    14401 taagtcagtg ctgctgcaaa taatacgtgt cttgccatcg gtacttcttc ttccgtcagt
    14461 agtccgcctt cactcaaaat ttggattgct cgccgagacg cgttaaattc gactggtaat
    14521 gtcaccaatt ggaaaagtaa tgctaaagaa aaagcgaaaa tcccgatatt gattaacgta
    14581 gagttccagc taagcaatac acctaccata atcaagggga acgaaatcat cgaaccaaag
    14641 ttagctgctg gaacaagtgc tgcacgaatc ttcaatggga cataacccga agcatcttgt
    14701 accgcatgtc cgcattcatg agctgctaca cctatcgcag caactgaagt agactgtgca
    14761 gttgcttgag acaaactcaa catcttatta cctgaattgt agttatctgt gagatctccg
    14821 gcaatttgtt gaacaccgac attatttatc tgttgacttt gtaatatata ttgtgctgct
    14881 tgtgtaccag tcacattgtt tttgcttctc acttgatcgt attttcggaa agtactattg
    14941 acgtacgctg acgcaattcc ggaaataact aatccaagaa tgaccaaaag aaatgtcgga
    15001 tctaaaaaac catacattcc ataaccatac attttcgttt cactcctttt cgtttacttt
    15061 attttatcat tagtagtagg aaaagaatgt gaaaagacct tgaatattct ttaattcttt
    15121 ttcacaaatt ccgatttcag tttcatcggt ccaaatcctt ctaccttgca atcgatatta
    15181 tgatcgcctt ctactaaacg gatgttcttg acttttgttc cttgtttaat ggcacctgac
    15241 gcacctttta ctttcaagtc tttaatcact gtcacgctat cgccctctaa cagacggttt
    15301 ccatttgcat ctaataccac taatccgtct tctatgacag tttcttgttc ttctttgttc
    15361 cattcgttcc cgcattctgg gcaaatcaac aagcctcgat cttcatatgt gtactctgag
    15421 ccacattctg ggcaatttgg taatgtcatc ttctttctcc tttaaaatct ttagtgagca
    15481 ctattgtaac ggaaaaagaa gatcttgaaa agatacattt gtgattttta ctacaaaagg
    15541 cacgttttcg aacgaagtat cattctgttt ttctgctctt atacgattgg catatatctt
    15601 ggatctcttt atagatttcc tctacttctg ctaaatcttt tgcattcgca ccacttgcta
    15661 atggatggcc tcctccatga tggcgtttag ccaattcatt gatgatcggc cccttagaac
    15721 gtaaacgtac gcggtaatac cctgaaggct gctgaacaaa aatcccccaa gccagcactt
    15781 cgtcaatcac tccaggaagt gaaacgatcg aagctgtctc tgaatcatcg attccatatt
    15841 tatcaagaat ttcttgcgga aggattattc ttcctgcacc attttcatcc acctggatat
    15901 tttgataaag ataaccggaa agtttagcga ctttcatcgg catttgttca atctctctgt
    15961 tcaaagcact tgcatcaaat ggataatttc tcaatatcga agcaatctct aatgtacggg
    16021 acgtcgtgga gggatacaaa aagcgccctg tatctccgac gattcctgca taaagtaacc
    16081 gtgcagcctt aacgtttatc tttaaagatt caggaaacat caaagcaaat tctgcaatca
    16141 tctcactaca gctacttgct tttgtattta cccacacaag atctccataa gggtcatcat
    16201 tgggatgatg gtcgattttg atcagttttt ctgccaactg atatctttga tcactgattc
    16261 tgggtgaatt agctgtatca gtcacgatga ctagcgctcc ttggtagaag acatcttcaa
    16321 cttcttgcat ttctgctaaa aagcgaagcc cttcaatcgt ttcacctact tgacgtattt
    16381 ctttttctgg gaaactttcc cgtaatagtt cggctaatcc tacttgtgat cctaatgcat
    16441 caggatcagg gcgcaaatgc ctgtggataa tgattttatc gtattttttt attgtatcta
    16501 ctatttcttg ctgtactgtc atttcactcc tgctttctaa gaacgttcca ttacttgaca
    16561 aacaacgatt gctttggaaa cgatggtgtt ttcaataaac acttcgatat ccagtttagc
    16621 ggaacgtctg ccgatttcca atacacgtgg acgaatgtct aattcgcttt ccaattggat
    16681 cagccgcaaa taatgtaggt tcacttgttc aatcaagata tttcgttttt ggttcatcac
    16741 cattgttttt tgtgcgacat cagaaatgat ttcgctaagt acgccaaaag aaatggttcc
    16801 aacgccattg accatttgtg gtgctactac gaatttgaac ttagggtttt ccaaacgatt
    16861 tccttcacgg tcagtatcaa tcgtatggat atttccagat atctgatccg aaatcgtatc
    16921 tgcaatctgc ggttgtcgct ggacaagctg catcgctttc attacatctt gccgggtaat
    16981 caagccttcc aatgtcaagt catcggaaac gaccggcatg acttctagcc catcccagat
    17041 catttgatgg ctaacagagg caatactcat tgttttcttt acactgattg gttctttcgt
    17101 catcacccga tcgatgatct ggtttggcga tttccctaaa acatcttttg ccgtaacaat
    17161 ccccatcaaa cgatgatggc ggttgacgac tggaaaacgc gaatgctgcg tttcttctga
    17221 caaacgctga taatcagaaa tcgtatctgt ctgctgcaaa taatgagtct tttcagctgg
    17281 catatagata tcactgacaa gcatgatatc ttttttgatc aattggtcgc ttaacgctcg
    17341 gttgatcatg gttgcgactg taaatgtatc ataagaggtg cgtaagatcg gcatttcaag
    17401 atcatcagct agctttgcaa tctcagcagt cgtattgaat cctcctgtga tcaatactgc
    17461 ggcgccatct tccaatgcta atttttggac ttccgcccgg ttgccgacga tcattagtga
    17521 tcctggcgta atatagcgtt ccatggcatg tttagtcatc gcgccgatga cgaatttatt
    17581 caagaccttg tctaatcctg ccgccccgcc taaaacgtcc ccttcaatga tcctgacgac
    17641 ctcgccaaat gtcaaacgtt caatatgttt ttttaatttg cgttcgatac ggatggtccc
    17701 tactcgttga attgttgaaa ctagtccgac attttctgca tctttgattg cacggtacgc
    17761 tgttccttca ctgacattca attcttttgc gatgcttctt acagaaattc gattgccaac
    17821 aggtaaactt tcaatatatt ctaaaatcaa atcatgtttt gtcgccattt tctgcctaca
    17881 cttccagcgt ttccgagacg ctcattattt ttccttcact atcttccagt aactctacaa
    17941 aagattcggg atcttgttca atgacaggga aagtattgta atggattgga actactttct
    18001 tggcacgaat tgtttgcgca gcctttgctg catcttctgg ccccatcgta tagttatcgc
    18061 caatcggcaa gaaagcgaca tcaatatcga attcttctgc aagcaatcct agatcactga
    18121 aataagcagt atcccctgca tgataaatcg tttttccttc tgcttgaata ataattccgc
    18181 taggctcgcc catatactga acgataccgt ctacttcata gcttgaacta tgctgtgcat
    18241 gtacgaattt taccacgccg aatggaaacg catgttttcc accgatattc ataccgaatg
    18301 aatctactcc tctgctgttc gcataatcag caatctcaac aatactgata attgtggctt
    18361 gattttcttt tgcaatagga atcatatctc ctatgtggtc actatgacca tgagtaatca
    18421 gcaaccaatc tgtctttact tcttctgcag ttaagtctgt caatggattt cctgtaataa
    18481 acgggtcaat catcacgcgt tggccatttt ccatttcaat tgttaaacag gaatgtccat
    18541 gatacgtaat tttcatattt tacacctcgc gttttctctg tatctttttt attaaaaacg
    18601 atttcatcac ttgcacatca atgaaaaaat atagctgcta aatatatttt cattggattg
    18661 atagtacagt atgtacaaag caattgtaac agataataca ggatgcacca agtaccaagc
    18721 agatataatc tcaagtttga ggtctctgct ctgcttagct tctcttctcg tcccttaact
    18781 aattaaatca gcgtaaatag gatggacgat cttttctaaa tcggctactt ttatccgaag
    18841 gcttctcccg atttcaccgc ttgacacaat tacttcttct ttttctttta aagaactgtc
    18901 tgctaaaaaa caataatcag gatgtgcgag ttttattcca ataggggtgt ttgctccgtg
    18961 gggatatcct gtatgcatta atacaaaatc cattccagga aaaccaactt tacgatcttt
    19021 cgtcacttgt ctcgtttttt tgtaatctaa gtgatcgttc aaaggaatca atgcgatgat
    19081 caccccagtt ttgtttcctt tcactacgag agttttacat atcaaagctg gatcaatccc
    19141 tcttttttgt agttcttctg tgaaagattc ttctaccaca tgactaaaat caagtggttg
    19201 atagctgatc ttttgttctt ctaaataacg ttcgactttt gtttgactca tcattgattc
    19261 actcccttta ttctatcgtt tattataaag cgagggatgc tgtattcaaa aattatttgt
    19321 ttttcgcaaa aaaagagcaa gacgagcact ctatagagag gttcatctta ctctttttgt
    19381 ttatttttta tttcgacgtt gagtagcttc cataatgact ggcaagatca ctgggcggcg
    19441 ttttgtttgt tcaaataagt aacggcctaa ttgatctcgg atgtcttgct ttaatttgct
    19501 ccactcaaaa tcattgcttt ctaaatgttt ctcaacgatt gtttcaatca tattgctgct
    19561 ttctttcatc agatcacggc tagcttttac gtaaacgaat ccccgtgaag taatttgagg
    19621 ttttgaaatg attttctttt ctcgccgact gatagtgaca accgctacga aaatcccgtc
    19681 ttctgacaag atttttcgat cacgtaaaac aatgtttccg atatcaccta tgcctaatcc
    19741 atcaatcatt acattttctg cacttgtagt tcctgcaaca ctcatacgct tgttcttata
    19801 ttccagcacg tctccgcgtc cagtaatata gatatttttg tatggcatcc ctactgcatg
    19861 agccaaatct gcatgagcag ctaattcacg atactctcct tgaactggca caaaatatgt
    19921 tggtttcatc aaattgatca ttaattgcaa gtcattagga ttggcatggc cagaaacgcg
    19981 catattctca gagatcaact tcactgttcc accagctcga taaatgatat cttccgtttt
    20041 cgccacgatg gtttccatcg cgatgcttgg cgtagtagtg atataaacca aatctccttc
    20101 ttcaatcttg acattacggt gtgtaccgtt tgccattttt tgcagagact tgatgggttc
    20161 acccattcgg ccagtttcta aaatgatcag ttcttctgga tcatagtttt tcatgtcttt
    20221 caacgtaaca agcagatctt cacttggtaa ttgaagtttt tctaaacgca tcgctgtgcg
    20281 aataattcgt tcgaaatctt gaccagtcaa gacaactttt cgttgtgctt tttctgctgc
    20341 atccaatact tgttgcactc gttgcaaatt acttgctaca caagcgacga tgatacgccc
    20401 ttcccaatat ttgatcgtat caaatacttc atctgctatt tgggcttcag aagctacagg
    20461 ctgaggattt tctgcatttg ccgattcact tagtaaggct aagactcctt ctttgccgat
    20521 ttccgccaat cgtgcataat ctgtttgata cattgggata gccgtttggt caaatttaaa
    20581 atctcccgta taaacgatac taccttcttt tgttttcaga ttgatcccca ctgaatctgg
    20641 tatcgtatga gtggtacgga aaaagctcac tactgtttca ccaaagtcta tttctgtatg
    20701 ctcatctacc acatggaaat ccttaaatcc tttggctgct tcattacgac tgacattcaa
    20761 ttttgctaac tcgatcgtca actctgtccc gaatactggt acagaaattt ttgacaacaa
    20821 gtaaggaagt gccccgatcg catctgcatg tccatgtgtt aaaaagacac cagctacacg
    20881 atcaatgttt tcttctaaat acgtaaaatc agggattacg acgtcgatcc ccaacaactc
    20941 attttcagga tattttagcc cacaatctaa aataaaaata tcctcgccca cttctgcgac
    21001 atacatgttt tttccatttt cacgaacgcc gcctaatggg ataattttaa tggtactcac
    21061 aaacttcacc tcttcttatg acatccaagt ttaaagatgt cgattttctt ctttatctaa
    21121 atatatgagc cattccaatg aagcatatgc aaaatcacta tttctcaact tatggagaat
    21181 cagcaggaaa attaccagca catccgcgtt ctgacagttg gatgggcatt ttattttgga
    21241 tatacttcat taacaaaatg gatcttttgc gaaacgctat agccagaata cttatataag
    21301 tattcagctg ttccccatta ttatacaaca aataaactaa atcgctagta tcagcaattg
    21361 ttagaacaac tatgcacgta aaaatccctc tgtagcatct aggatttctt ttgctattcc
    21421 tctttcattt cttcttcgtc ggtttgaaac atactttttt cattttacca cattttttgc
    21481 aataactata gtgacttttt atatatacag aacctcatgt tgatggtgtg caaacagcat
    21541 aagatttaag ctagctttaa gataggttgt ctattatcaa atgcatctac taggagggat
    21601 gcaaaatatg aattttatga aacgtgcatg gaaaagtaca aaagtaaaat ggggacgttc
    21661 attgttattg tttgccgtgt tcacagccat tcttattttt gtgctagctg gcctcacgat
    21721 tcgaagcgct gcagaagaag cagcgaatca agcacaaaaa gaagttggag caacagtgac
    21781 tttaagtgcc aatcgagaag cttcatttaa aaaacaggaa caaacttctt caagcggttc
    21841 taccactgaa gaacgtccag atccaggcag tttttctctt acaccagttt ccttaacgga
    21901 cgcggaaaaa atagctgaat tagataatgt aaagagttat tcctttgaaa gttccgcctc
    21961 agctatggca aaagatggaa taactgccat cactagttct gattcaagtg aagacagttc
    22021 agaaactact gatgcaggcg gtgaacctgc tggaggaatg ggcgggaatc cgcaaatgac
    22081 tcaagcagac tttcaaatca gtggtgtgtc agcaactgca caaaatagta atttttctga
    22141 tggaacagca aaaatcacgg atggtgaagg aatagatgac agcgacaaag gcacaaacaa
    22201 tgtagtcatt gattctactc tagcagaaac aaatgacctg gcagtcggag atactttcac
    22261 aatcacgagt actgaagatg aagataccac ttatgaaatg acgatcaaag gaatttatga
    22321 atccagcgaa acaagcggca gtatgggtat gaaattcaac ttcatgaatc cgtcgaatac
    22381 actttacact tcttacactt ttgcaaatga actaaatggg acatctgacg ataacacgat
    22441 agattctgct atttacactc tatctgatcc agataaaatg gatgagtttc tcaaagaagc
    22501 tgagaagctg atcgacacag atacatttag cttacaatca aatgattcca tgtatcagtc
    22561 aatgttggaa cctttaaaca acgtggcaag tttttctaaa aatgttgttt tactagtggc
    22621 tgtagccggt attatcatac ttactctgat cattatgatt accattcggg agcgtcggca
    22681 tgagttaggc gtcttgctat ctttaggaga atccagaagt aaaatcattc tccaattgtt
    22741 taccgaagtg gctatttgta tgattcttgc attaggagtt gcttcgctta gcggaaacgt
    22801 cgtcgcgaat gccgtgggac agcagctact tgaccaacag acagaaacca cgacacaaaa
    22861 ccagcaagct cctggcggtg gacaaatgcc aggtaacaga caagaacaag gcggtcaacg
    22921 cgatggtcaa caaggaacca cgaataatcc atttgctgtt tctgatcaag tcagcgaatt
    22981 ggaaatcaat gtcaaacctg cacaattggg actattagcc ggagtagcat ttggaatcag
    23041 tctgttttct gtctttttag cttctatagg gatattaaga ttaaacccaa gaaaaatcct
    23101 attaaactaa aggaggaatt tgatgttacg gacagaacaa ttgggatttt ggtatcaaga
    23161 tccgcaagat gcgctttata aagatgtaga actgaatttc gaagctggaa aaatgtacgc
    23221 tattttagga gcaagtggtt ctggaaaaac gacttttctt tcgcttattt ctggactaga
    23281 tgcaccaaaa gaaggaaagg tatactatca agatcgttca ctgaacaaaa tcgggttaag
    23341 aagttatcgt aaaaatgatg tctcgattat tttccaagct tacaacttgc ttccttattt
    23401 atcggctcta gacaacgtca tagcagcaat ggaaatcact aagaccaagc aagagaatta
    23461 tcgacaagtt gctttagatc gactggctcg tgtaggaatt gatgaaatat tagcaaaaaa
    23521 acaggtgacc aaactttcgg gaggtcagca gcagcgagtc gctatcgttc gtgctatctg
    23581 ctgtgaacat caactaatcg tagcggatga gcctactgga aatctagatg aaacaacttc
    23641 acaagatatc gtcaaacttt tccaagaaat cgcccatgaa caaaatcgct gtattatcat
    23701 cgtgactcat gagattgaag tagcaaaaag ctgtgatgaa gtctttgaac taaaaaataa
    23761 aaaatttcaa aaaatcgaag tttaaaaaac aggataatga tcaaataaaa aatccgaacc
    23821 atatctgtct cgcagatatg gtttggattt tttatttgct tgattcaatt agtaataagt
    23881 ggtttttatt ttttttcgtc ttttcgagaa aacttgtctt tgacttcgtc gcctaaattt
    23941 cgagcttttt ctttgatgtc agcacctagt gattgtgctt tcccttctgt ttctttgctc
    24001 ttgtcgtctg ttgctttgcc gtatgcttct tttccttcac cgactacttt atctttcata
    24061 tctttagaac gtccttttaa atctgccatt gtgcttcctc ctcttagttt gtttattgat
    24121 ctaccttcag ggtaacatca acaacagata cagacaaaca gaacgctcgt tttctaaaaa
    24181 agatagttag agctgttgat taaaaacttt tttcgaaagt cccccatact ttgacttctt
    24241 tttcttggac cattatttct cctttagcga taactgtatc aatatcaagc gtttctttat
    24301 ccaagataca cagatctgca tcagcaccaa tttcgatttt tcctttttta tccaatttca
    24361 atatacgtgc tggattactg gttatagctc gtaaggcgat ttccaaaggg atactttctt
    24421 tttgcacagc ttctttgatc cctactagca atgccttggc acttcctact cctaacccta
    24481 ggaaatgatt gttttcatca aaataaggca agcttccttg tccatctgag gtcatcgtga
    24541 aattatctaa agaaacatct tcctcgatca atcgtttcag tgctttgctg aagcgaactt
    24601 caccatctgt cttctcccaa aaatcaggat cttcgctagc agtgaagtca atcgtgccac
    24661 cacgtttggc aaatgccacg caagcttcaa ataattctgg tgtgcgattt gcatgagtag
    24721 gcaaaaattg tgtgatcgga atttctgttt cttctacgat tcgtgtcaat agctctagct
    24781 ttctttttcc tccgcctaag tggacattga tgatcccagc ttttcccgat aacatgcctc
    24841 ctacacgagc atctgctact gcacgagcga actcttcaaa acttggctga gaagaacggt
    24901 gatcggatac tgcgatttct ccgattccga taattttatc aattgtcaaa aagtctttga
    24961 tgatagaatc tgttactgtt tttaccggca atcggtacga accttcgtat acataagtgg
    25021 agattccttc tgcttccaga cctttcgctt tgctgatcaa cgcagtcata tctctacctt
    25081 ctccgtctgt tcccaaacaa ccgacaactg tcgttacacc agctgtagtc aattggctca
    25141 atgtgacttc tggcgttcga ttttgataac cgccttctcc tcctccacct aaaatatgga
    25201 aatgacagtc aatgaaaccc ggggttagta cttttccctc acctgaaagt tcttctactt
    25261 ccactccttc gtagccgcct tctagctgtt cttctacagc caaaatcttc ccaccggcaa
    25321 tcagtacatc catttttcct aagtaatctg gtgcaaacac ttcaatttgt cgaatgattt
    25381 tcatacattt ttcctctttc tttaagtata actgatgata ggatcttaaa aaataatcca
    25441 aacgatagga tctttactcc tttgatttta ccgtaaaatg acggttcttg gagtccttat
    25501 ctactgtttg gattactttt ttatttacag gtctccagta ctactgcaga cctattgcaa
    25561 tggcaatcat gatgaaaata gttcctaaaa caaacaggaa tccttgcatc ttgatttgga
    25621 atcttgccca ttttgcccat tcgatccgtg caacacctag cacaccaatc aagctggctg
    25681 atacgggtgt aaaagcgtcc ataaaacctg aaccaagctg ataagcaaga actgctactt
    25741 gtctggatac acctactaaa tctgccagtg gagccatgat tggcatagtc aaagctgctt
    25801 gtcctgaatt tgatgtaaca accaagttga acaaactttg gaagatgtac atcgcccaag
    25861 caccaatgac agctggaaca ccggtcaacg ccgtcccgat cccatgcaaa atcgtattca
    25921 aagctgaagg aacagatgca tcagatcctc ccaagacaag aagaatccct ttggccatac
    25981 ctactacgat tgctgtacca gctaaatcag ctgcaccaga ttggaaagaa gaagcgatgt
    26041 cattgacttt cattccatct aatttgaaaa gaacagcaat gattccagca actaatccca
    26101 tcacaaagaa ttgagaagcg atttctggaa tgtaatagcc tttttgagtc acgccccaaa
    26161 cgatccagac aaggacaact aacatctcta ataagatcaa tttatgccca agcatgaatt
    26221 ttttattttc atcagatgtc tttttgatat gagaacggaa ataatcatct gatttgtaag
    26281 tcaacgaact ttctggtttt ttacggattt tttctgcata tatcatcata taaccagcgg
    26341 ctaatgcagt aacaactacc cacataatca atcggaatgt cgcaccagac aacacgggga
    26401 ttccagcaat cccttgagcc accgctacgc taaacgggct catccaagag gtcgcatttc
    26461 cgacttgaga tgcgacataa gtcacggtta ctgctacgat cgagtcatat cctaacgcaa
    26521 tgacaaatgg aaccatcacc attgagaatg gaattacctc ttctgccatc ccgaatgtag
    26581 cgccaccgaa tgaaaaagca aagaataata gcggtaaagc taaacgctcc agtcccttag
    26641 ttttactgat aaacgcatag atacctgcgt cgatcgctcc ggtacgcata ataataccaa
    26701 aagcacctcc aacaacaagg atcaatgcag cgatccctac agcagatcca tacttatctc
    26761 cagatactag tccttcaaaa acaaaattta agaaaccaaa gccgccaaaa tcatctgttc
    26821 cccatatttt ggctgtttta tgcaattttt tagaagtatc atagatattt tcgccgtact
    26881 gatcgtaaag cacatcatct gtcaaggaga tttcatctag tttttcctgc gtaagatttt
    26941 tttctccgtc agcaatgact tttccaatcc tttttccgga acatccagtt tttcacgttc
    27001 tgctggatga tcttgcagtt cttctaattg atcaaacaca tacgacttat ccagctcgta
    27061 agcataacgg aaggaatctt gacgtaaaac agtacgagta cttgtttcgc catttgcatc
    27121 tttgtattca atatcttgcg tgctgaactt tcctgctgga acgacaaaag tcagtatcca
    27181 tgcaaagatg actacaccaa aaataatgac atacgtatgg ggtgtcgata atttagtgaa
    27241 gtcgcgtttc ggttttcctg aagaatttgt tttcttcaaa tttatttctt cctttctttt
    27301 ttcagtaatt ttgatgaata aaaaatggat aatcaaatga attatacagt aaaaatgaca
    27361 tttccacatg aataatttat tagaaaatca cttttttcta ttttgttttt ttctcctatg
    27421 tat
//
LOCUS       DAZUMV010000023        26197 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000023 DAZUMV010000000
VERSION     DAZUMV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.023"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..1091
                     /locus_tag="ACG8FK_000915"
     CDS             87..1091
                     /locus_tag="ACG8FK_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096542.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNIKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1092..2036
                     /locus_tag="ACG8FK_000916"
     CDS             1092..2036
                     /locus_tag="ACG8FK_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096543.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2036..2998
                     /gene="opp4B"
                     /locus_tag="ACG8FK_000917"
     CDS             2036..2998
                     /gene="opp4B"
                     /locus_tag="ACG8FK_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5096544.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3025..3939
                     /locus_tag="ACG8FK_000918"
     CDS             3025..3939
                     /locus_tag="ACG8FK_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096545.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3965..5746
                     /locus_tag="ACG8FK_000919"
     CDS             3965..5746
                     /locus_tag="ACG8FK_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5096546.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6094..6780
                     /gene="rnc"
                     /locus_tag="ACG8FK_000920"
     CDS             6094..6780
                     /gene="rnc"
                     /locus_tag="ACG8FK_000920"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS5096547.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6800..10381
                     /gene="smc"
                     /locus_tag="ACG8FK_000921"
     CDS             6800..10381
                     /gene="smc"
                     /locus_tag="ACG8FK_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS5096548.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10390..11199
                     /locus_tag="ACG8FK_000922"
     CDS             10390..11199
                     /locus_tag="ACG8FK_000922"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5096549.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11210..12208
                     /gene="ftsY"
                     /locus_tag="ACG8FK_000923"
     CDS             11210..12208
                     /gene="ftsY"
                     /locus_tag="ACG8FK_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS5096550.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12395..14662
                     /gene="gshAB"
                     /locus_tag="ACG8FK_000924"
     CDS             12395..14662
                     /gene="gshAB"
                     /locus_tag="ACG8FK_000924"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS5096551.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14751..15203)
                     /locus_tag="ACG8FK_000925"
     CDS             complement(14751..15203)
                     /locus_tag="ACG8FK_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS5096552.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15353..16303
                     /locus_tag="ACG8FK_000926"
     CDS             15353..16303
                     /locus_tag="ACG8FK_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5096553.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16296..17255
                     /locus_tag="ACG8FK_000927"
     CDS             16296..17255
                     /locus_tag="ACG8FK_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5096554.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17252..18007
                     /locus_tag="ACG8FK_000928"
     CDS             17252..18007
                     /locus_tag="ACG8FK_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096555.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18030..18983
                     /locus_tag="ACG8FK_000929"
     CDS             18030..18983
                     /locus_tag="ACG8FK_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5096556.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19627..19923)
                     /locus_tag="ACG8FK_000930"
     CDS             complement(19627..19923)
                     /locus_tag="ACG8FK_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS5096557.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20278..20634
                     /locus_tag="ACG8FK_000931"
     CDS             20278..20634
                     /locus_tag="ACG8FK_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5096558.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20644..21543
                     /locus_tag="ACG8FK_000932"
     CDS             20644..21543
                     /locus_tag="ACG8FK_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5096559.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22063..22791
                     /locus_tag="ACG8FK_000933"
     CDS             22063..22791
                     /locus_tag="ACG8FK_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5096560.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22936..24963
                     /locus_tag="ACG8FK_000934"
     CDS             22936..24963
                     /locus_tag="ACG8FK_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS5096561.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            25071..26168
                     /locus_tag="ACG8FK_000935"
     CDS             25071..26168
                     /locus_tag="ACG8FK_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5096562.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         8743 a         4565 c         5558 g         7331 t
ORIGIN      
        1 acctttgtta taaaacgatt tattctgtgt aatgtccttt tttattaaga aaaacttcca
       61 tttaggaagc agagaggggt attcgattgt ccgataacca attattagaa gttcagaacc
      121 tacatacagg attccgactc aaagatgact attacgatgc agtagatgac gtttcattta
      181 ctttagacaa aaacgagatt ttagcaatcg ttggggaatc tgggtgcggg aaaagtacct
      241 tggctactac gattatggga ttacttgatc ctaataacat aaaaattaca ggtgagattt
      301 tatataacga tctgaattta atcgacctga acgaaacgct ctataataaa atccgcggaa
      361 atgatatcgg aatgattttc caagatccgt tgtctgcatt aaatcctctc atgagaatcg
      421 aagaccagat caaagaagga ctttcctatc acacaaagat gaatgcagaa cagcgtcaag
      481 ctcgtgcact agaattgcta gaacaagtag ggattcctaa tcctgcgaga gttggacgcc
      541 aatatcccca tgaactatca ggcggtatga gacaacgtgt catcatcgca atcgcaatcg
      601 cctgcaaacc tccaatcgtc attgcagatg aaccgacgac agccctagat gtaaccatcc
      661 aagcacagat tcttgattta ctgaaagacc ttcaggaaga aacaaaaaca gggatcatct
      721 tgatcactca tgacttagga gtcgtagcag aaatggctga tcgagtggca gtgatgtatg
      781 gcggacagtt cgttgaagta gcaggtgtaa aagaactgtt tgatcatcct aagcatcctt
      841 atacacgctc tttactgaac tctataccac aagaaggaaa tcatgatgca gaactccatg
      901 tgatcgaagg tgtcgttcct tctctgaaaa acatgcctcg taaaggttgt cggtttgcac
      961 cacgtattcc atggatacca gcaagcgatc atgaagaaga gcctatcctt catgaagtcg
     1021 caccgaacca cttggtacgt tgctcctgct ataagcattt ccactttaga gacgaaaaag
     1081 gagaggtgta gatgacagaa ttaatcacaa tcaaagacct gaaagttcat taccctattc
     1141 gcagtggatt cttcaatcga gtaacggatc atgtatatgc tgtagatggt gtggatttta
     1201 ttatcgaaaa aggaaagaca tatggtttag tgggagagtc tggctctgga aaatctacta
     1261 cgggaaaagc cgttgtggga ttagaaaagg taacagccgg acaaatcatg tatgaaggaa
     1321 aagatgtaac gaagcgaagc aaccgcaaaa aaatgggtta taacaaggat gtgcagatga
     1381 tcttccaaga ttcaatgtcc agtttgaatc ctaaaaagcg agtgttggat atcattgctg
     1441 aacccatccg aaattttgaa cggttaagcg atcaagaaga aaagaaaaag gtaaaaggac
     1501 tgctagatat cgttgggatg ccggaagatg cgctatataa atatcctcat gaattttcag
     1561 gcggacaacg tcaacgtcta ggagtagccc gtgctgttgc taccaatcca aaactgatcg
     1621 tagccgatga acctgtatct gctctggatc tatctgttca agcgcaagta ttgaatttca
     1681 tgaaacgaat ccagcaagaa tacggcctca gctatttatt catttcacat gatttaggtg
     1741 tagtaaaaca catgtgcgac aatatcgcaa ttatgtacaa aggtcgattt gtagaaatcg
     1801 gtacaagaga agacatttat aatgacccgc gacatatcta tacgaaacgt ctgctttcag
     1861 ctattccgca aatcgatgta gaaaataggg aaagccacaa agaaaatcga cgaagagtag
     1921 aacaagagta tatccaaaac caaaaagaat actatgatgc gactggtcga gtctacgatt
     1981 tgcgtacgat cacaactaca cacaaagtgg cgttgaaaga tggaggtgca agctaatgtg
     2041 gaaaacgatt ctgcgacgtg ttttattgat gatcccgcaa gttattattt taagtgttct
     2101 gatcttctta ttagcccaag cgatgccggg ggatccattt accggtttga tcaatccaaa
     2161 tcaggatccg gcagtgatcg aacagatgcg acaagcagct ggcttgaatg acccatggta
     2221 tgtccagtac ttccgttgga tcggaaatgc tttgcaaggc gattttggtc aaagtttctt
     2281 atacaaacgc tctgttgctg acttgatcgg cgaacggatc atcaacactt tatatctatc
     2341 gatcttgaca gttgtcctta cttatttgat tgctgtgcct ctggggatgc ttgctggccg
     2401 gtatcaaaat tcaatcttgg ataagattgt cgtaatctac aacttcttta gttttgctgt
     2461 tcctttattt atcttcggct tgatcatgct atttgttttt ggttatcgtt taggctggtt
     2521 cccaacaagt ggatcacaaa cactaggttt gcaaggtgga tttatcactc aatggataga
     2581 ccgtttgaaa tatattctgt taccatcaat tacccaagca tttttggcga ctgccgtcac
     2641 aatccaatat ctgcgaagtg aagtaatcga ttcgaaatcg ctcgattttg tcagaacagc
     2701 ccgttccaaa ggggtaccga cgaataaagt attcagtcga catattttcc gaaatgcagc
     2761 gttgccgatg gcttctcaaa tggggtatga aatcacttcg ttgattgctg gttctgtagt
     2821 tatcgaaaag atttttggtt atccgggagt cggaaaacta tttattgatt ccatcggcca
     2881 acgggactat accgtgatca catccttagt attgatttta ggaatcgcga cgctagtggg
     2941 gacattactt tcagatatca tcatgagtat cgttgacccg cgtattcgga ttcaataaaa
     3001 ccaatcagaa aggagaatgg atcgatgagt gaaaaaaaca atacagcagt tcaagaaagt
     3061 attcctccta tggggatacg aatgattgct cgagaattta aaaaagataa aattgctatt
     3121 ttttcattag gtttgctagt ggttttatta ttagtgatct ttatcggtgc gatgctgacc
     3181 gatcaagaca aagtgatgta tgttagtatc ttcgacaaat acgctgcacc aggaacagtt
     3241 tcgacacaag gacaaaagtt cattctggga gcagatgaag gcggacgtga tgtattagga
     3301 caattgatca taggtgctag aaactctgtt acgatcggat ttgcgatcac gatccttact
     3361 tcgatcatcg gggttggatt agggatcatc tctggctact acggagggat cattgacaac
     3421 atcttgatgc ggatcgttga ttttatcatg attctaccga tcatgctgat cattatcgtc
     3481 ttcgtatcga ttatttccag atacaatgtc tggtccttta tattcatcat gagtgcattt
     3541 tattgggtag ctaaagcccg attattccga agcaagacat tatctgaagc aagaagagat
     3601 tatgtcagtg cctcaaaaac aatgggaacc aatgatttta aaatcatgtt ccgcgaaatc
     3661 atgccgaatc taagttcatt aatcattact aatttgacga tgaactttgc ggcaaacatc
     3721 ggtatcgaga caactttgac tttcttaggt ttcggcttgc cggcaaatgt acctagttta
     3781 ggtacattga tcggttatgc aagtaacgga gaagtcttag tgaatcggca atggatctgg
     3841 ttacccgcat caatccttat tttagttttg atgttgagta tcaattatgt tggacaagca
     3901 ttcaagcgtt ctgcagatgc gcgccaacgg ttaggctagt atagaagcga agggggaaat
     3961 aggaatgaag aaaacgattt ttggatttgc agcgctgatg tctgctgtcg ttcttgcagc
     4021 atgcgggggc ggaggtacag atacctctgg aaacaactct ggaggcaacg gcaataacaa
     4081 agaggaatta acttttccaa ttgcaacaaa aaataatgat gatgcaatcg aagacggcga
     4141 attagaagct gcggtcgcaa cggacagtca gtttaaagga ttgttccagt gggagtttta
     4201 ccaagattca tatgatgcag cttttatggc tccttctcat gaaccgctat ttgcaaacga
     4261 tgacgatttc agaatcacag acgacggagc tgcaacgctt gatctagatg aagatacgaa
     4321 aaaagcgaca atcaaagtaa aagacaacgt caaatggtca gatggtgaag atgtaacggc
     4381 ggacgatgtg attttccctt acgaaatcat tgggaataaa gattacactg gtatccgtta
     4441 tgacgacact ttccgtaaca tcgtaggtat ggaagaatat catgatggaa gtgccgacac
     4501 aatctccgga atcaaaaaaa tagacgacaa aacagttgaa atcgaatata aagaagtaaa
     4561 cccaagcatg cttcaatcgg gtggtggtat ctggtcatat gcagcaccaa aacatacgtt
     4621 agaaggtgta gcaatcaaag atatggaatc aagcgatcaa gtacgtaaaa atccagtgac
     4681 ttttggccct tattacatga gtaatatcgt tgcaggagaa tcagtagaat acttgccaaa
     4741 cgagcactac tggaatggta cgcctaaatt gaaaaagatc acgatgaaat ctgtaccgat
     4801 ggcaagtgca actgaagcat tgaactctaa attatatgat atgattttcc aaatgccaac
     4861 agatacttac gatacctata aagaaattgc tggctataca aacttaggcc gtgaacaaac
     4921 ttcttatact tatctaggat tcaagttagg taaatggaat gcagatacaa agagtgtgga
     4981 atacaatcca gattcaaaaa tggctgataa atcattacgt caagcaatgg gctatgcatt
     5041 agataataat gcagtaggtg aacgtttcta tgcaggtttg agaagcaatg cgacttcatt
     5101 gattcctcca gtattccagt cataccatga tgatgagatg aaaggattca cacaagatct
     5161 agacaaagcg aaaaaactat tagatgatgc tggctacaaa gatgtggatg acgatggttt
     5221 aagagaagac aaagatggaa ataaactaac aatcaacttt gcttcgatgt ccggtgggga
     5281 aacagcgcaa ccattggcag attactatat ccaacaatgg aaagaaattg gtttggatgt
     5341 ccaatacaca acaggtcgtc tgatcgaatt caattctttc tatgataaat tagaaaacga
     5401 tgatcctgaa attgatgttt accaagctgc ttggaataca ggaactgatc caaatccaag
     5461 tggactatgg ggaccaaatg ctgcgttcaa ctacactcgt tatgaatcag aagaaaatac
     5521 aaaattgatc aacgatatca gttcaagcaa agcatttgat gaagagtacc aaaaagaagc
     5581 atatcaaaaa tggcaacaat atgcatttga agaagcattt gcgattccaa cgttgttccg
     5641 taatgaagta ttgccagtca acgaccgtgt caaagactgg gattggtctt atgatgtacc
     5701 aaacccatgg gcaaatgttt ctgttacagc agattcacgt tcataatcac taaaaatgac
     5761 cgaaaaaggg cgatgtcctt tttcggtttt ttgatatttt tctgtttatt tctctaatat
     5821 tcaggtcaag gcgctgattt catgacttct ccaataaacg gtgttcaaag tccagtttct
     5881 cggtattaag tcaaatttat gcaaaaatat ggaaagccat tttcacaaaa ttttttcttc
     5941 atactcgaaa ctaaacgaat ttttcacaac ttctcgtcaa tcactaggat tattaaagct
     6001 ttacttacct tttgaccgaa ccatttcaaa tttgacaaaa aaccagtata ataaggaagt
     6061 atcgaaaaaa tatccataaa aggaatgtgg aaaatggaca accagctgac aaaagaacta
     6121 aaagaaaact ataacatcgt ttttcacgat ctaaatttgc ttgaacaagc ttttacccat
     6181 tcatcctatg tgaatgagca tcgcaatctg caattatcgg ataatgagcg acttgaattt
     6241 ttaggagatg ccgttttaga actgatggtt tctcagtatt tattccgttt atatcctcat
     6301 ttgccagaag gaaaattgac aaaaacacga gctgcgatcg ttcgcgaaga cagcttgtcg
     6361 aaatttgcca aagaatgtca ctttgatcaa tatgttttgt tgggcaaggg agaagagaat
     6421 tcaggaggac gtacgcgacc agcattgctt tgcgacctat tcgaagcttt tttaggcgcg
     6481 ttatatcttg accagggatt cgacacgacc catgcattca tcgaaaaagt gatttttcct
     6541 aaagtcaaag caggtgcttt ttcacatgag atggatcata aaacaaaact acaagaagtc
     6601 ttgcaaaaat caggtgatgt atcaatcgaa taccgattga tcaatgaaga aggtccagct
     6661 catgaacgag tattttggat cgaagtttat gtagatgatc aattgattgg cactggacaa
     6721 gggaaatcga aaaaattagc agaacaagct gcggcggaaa atgcattggc agcactgtaa
     6781 aagtaagtga gggaaagctt tgtatttgaa acgaattgaa atagccggtt ttaaatcatt
     6841 tgcagacaag acagtcattg attttgaaaa tagcgtcaca gcagtggttg gaccaaatgg
     6901 cagcggaaaa agcaatatta cagaggctat ccgctgggta ttaggagaac aatctgctaa
     6961 aagtctgcga ggcggaaaaa tgccggatat catctttgcc ggttccgata cccgtaagca
     7021 gctgaatatt gcagaagtca cagtgatttt agacaatagt gatcattatc tgccattgga
     7081 gtacaatgag atcagtgtaa cccgccgtta tcgacggact ggggaaagcg agtttttcat
     7141 caataaacaa agctgcagat taaaagatat ccaagaattg ttcttagatt ctggattagg
     7201 aaaagaatcg ttctcaatca tttctcaagg aaaagtagaa gcgattttca gtagtaaacc
     7261 ggaagaccgt cgtgggatct ttgaagaagc agctggtgtc ttgaaataca agcagcggaa
     7321 aaagaaagca gaacagaagc tttttgaaac agaagacaac ctcagccgcg tccaagacat
     7381 catccacgaa ttggaagaac agttaactcc tttggccgcc cagagtgagg cagcaaagga
     7441 gtttttacgg ctaaaagaaa cactgactca aaccgatgtc tctttgatgg ttgctgaaat
     7501 caagacagca aaaaaagatt gggacaacaa acaagcacag cttgcgaaat tcaatctaga
     7561 attaggcaaa ctatctgagt cgatccaaga acaagaatcc attttggcga agcagcgtaa
     7621 agaaaatgcg caagccgatc gtctgatcga aaaaaatcag caagtattat tagatctttc
     7681 ggaaaaactg aagcagacag aaggacaaaa agatgttttg caggagcgga caaagcatac
     7741 acaaaaaagc tcgcaagaat atcaaacttc attagcagaa gcacaaaaaa aggttaaaca
     7801 ttttgaaaag cttcaagaaa gcttgatgaa agcagcagca gaaaaagaaa cagagatcca
     7861 aaaagctgaa gcaaatctca taaaaacaca gcaagaatta gaaaaatacc aaaaatcaac
     7921 gaaagaatta ttggcagaat taagagatca atatgttgat ttgatgcaag aacaagcagc
     7981 tgtaggaaac gaactgaagt atctagaacg tcaatatata caagaaacag caaaaagcaa
     8041 gcagacgtta gccaagcaat cggaagtaga agcatcagta gaccgattga tcttacaaaa
     8101 agaagaattg acacaaaagc aggcgcagct aaaatcatct ttaactgaaa caaaagagaa
     8161 gttagaaatg atccaacaaa acgggaaaaa gttccaagaa gcattagcaa aagaacagcc
     8221 taaaatgtat caattaatga atcaagtcca gcagctacgt gctcgtaaga aaagccttca
     8281 agaaatccaa gaaaactatt ttggttttta ccaaggggtt cgattggtct tgcagcataa
     8341 acagcagctt tcgggtattg tcggagcagt ggctgaactg atcgatgttc ctgcagattt
     8401 cacgttagct atcgaaacag ctttaggtgg ggcagcccag catgtcatcg ttgaaaatga
     8461 aaaagatgct cggcaagcca tcacgtattt gaaacagcaa agaggaggaa gagcaacatt
     8521 ccttccactg acgacaatca aaccaagaca attaccggct catatattga cacaagcagc
     8581 agctgtagaa gggtttatag ggattgccag tgaacaagtc agttatcccg atcaaatcca
     8641 aactgtggtt caaaacctgc taggaacgat ccttttagcc aaagacttaa ctagtgccaa
     8701 tgcaatcgcg caaacgatcc gctatcaata tcgagtggtt tccttagaag gtgatgtgat
     8761 gaatgccgga ggatccatga caggtggagc gaataaacga ggaaaccaag gcagtttgtt
     8821 tgtacaaaat caagaattga aacagttgac tagtgaattt gaagaagcag ataaacaact
     8881 ccaagcacaa gaaaaaaaag ttcaggagct gcaacaagaa acagctcgtt tagccgaaga
     8941 acaagaagtt ttgcgtacac gaggcgaaca attacgtttt gaagaacaag aagcgacaaa
     9001 tcagctacaa aacatcataa atgaacttga acgttttgaa aaagaaaagc aaatctcaac
     9061 ttttgagaca cgtgaactgc agcagtttat cgaagattac gaaaagcaaa cgaacgaatt
     9121 gaaagacaaa caaacagatc ttgaatcgca acgacaaaaa atcgacgaag aaatcaaatc
     9181 gttaagtcaa gaaagcgacc agatggaagc cagaagagca caagtacaaa gtcaaaaagc
     9241 acaagaacaa gcagatcttg ctgtcttgaa agagcagttt aatcatctgc agatccagct
     9301 ccgcggtgcc cgagtccaaa aagcagaggc aaccgaacgt caagaagcga tagaaaagca
     9361 gttggcgaca ttgacagcag acttttctga tcatgaagtc acagaagaaa gtttggaaaa
     9421 acaaattaac gaattatctg ctcagcgaga aacattaaaa gccgagttgg caaaagccaa
     9481 agagcagcga gatagaaggc aaaaggagat cgataagctt gaagcagtct tggcagagcg
     9541 taatcgtgag caaaaagcac gactgtcgga acaatccaaa ttagaagtcc aaaaagaccg
     9601 agctgaaatg attttagata atcatttgac ctatctgcag tcagaatatc agctgacatt
     9661 tgagaaagct tcccaagact atcaagaaac aaccgatatc gaagacagta gaacaaaggt
     9721 ttcatcctta aaagaacaga ttgaaaaact gggaccagta aacttaaatt caatcgaaca
     9781 atacgaacaa gtcagtgaaa gacatacatt cttagcaaca cagcgagatg atctgttagc
     9841 agcgaaaaac caattgtttg aaacgatgga tgagatggat gatgaagtgc ggacaagatt
     9901 caaagaagta tttgaagcta tccgtcaaga attcaaaatt gtctttccaa atatgtttgg
     9961 cggcggtaaa gctgaattag tactgacgga accaactgat ttgctgaaaa caggtattga
    10021 gattgaagta caacctcctg gcaaaaaact gcaaagtcta agtttgcttt ctggaggaga
    10081 acgagcactt acagccatcg cgttgctctt ttccattatc cgtgtctgtc ctgttccgtt
    10141 ttgtatctta gatgaggtag aagcagcgtt agatgaagcc aacgtgaaac gttttggtcg
    10201 ttacttgagc gatttccaag atgacacaca atttatcgta gtcacacacc gcaaaggaac
    10261 gatggtcgca gcagatgtac tgtacggagt gacgatgcag gaatccggtg tatcaaaaat
    10321 cgtttctgta cggatggaag atatcaatga agaggcgaaa atcaacgaaa cgaaagtgta
    10381 ggaacgaaaa tgatcaaatt agtagcaatc gatttggacg gtacattatt aaatgaacaa
    10441 aagctgattt cagatgagaa taaacaagca ttagcacaag caaaacagca aggggtcaag
    10501 atcgttctat gtacaggacg ccccttagct gcgatggcgc attatctgca agaattagga
    10561 ctggtagatg aaggtgacta cagcatcact ttcaacggcg gtttggtaca aaaaaatgat
    10621 actggtgaaa tcatggaaaa gaaagtgatg gaagtatcag atatccaacg cttatacaaa
    10681 ctggctcagc aattggatct gccactagat gttttgtctg acaatgtggt tttacagctg
    10741 cctagtgccc caaggaagca atccttgtac aatgtgttga ataagctgct gcaattccaa
    10801 ccagcggata taagcgatat tacagatgaa tttgtgctta ataaggcagt aatcgcttat
    10861 gaccaagaag aattagatcc taaaatcaaa gaaataccag cagaattcca tgaaatgtat
    10921 gagatcatca aaacaagaag tatgcttttg gaatttatgc caaaaggtgt tacaaaagct
    10981 tatggtattt ctttattggc aaaagatctt ggtttgaaac aagaagaaat catggcgatc
    11041 ggtgatgagg aaaatgattt accgatgatc caatatgctg gactaggcgt agcgatggag
    11101 aatgctgtgc ctttcgtcaa agaagcagca gatttcgtga cagccagcaa tgaagaaaat
    11161 ggcgtagcca aagtgatcca aaaatttgta ttagagtagg gggagtcaaa tgggattttt
    11221 tgataaaatc aagcgtgctt ttactggcga agagaaaaca gaagaagttg aagtacagga
    11281 gaagtacgac aagggtttag aaaagacgcg taaaacattt ggtgaacgga tgaatgagtt
    11341 gtttgccaat tttcgaacag tcgatgaaga cttttttgaa gaattagaag aaacacttat
    11401 cggtgcagat gtaggttttg aaacagcgat acgaattacc gaagcattac gtcaagaagt
    11461 gaaactcaga aatgcgaaaa aatcggcaga agtccaaaat gcgatcatcg agaaacttgt
    11521 ggatctctat gaagcggaag gaatcaatga agtaaatgaa ctaaatatcc aagaagacgg
    11581 actaacagtt attttgtttg tcggtgttaa tggtgtaggg aaaacaacaa gtattggtaa
    11641 acttgcccat cagttcaaaa acgaaggtaa aaaagtattg atggcagcag cagatacctt
    11701 ccgcgctgga gcaatcgacc agttagttgt ctggggcgaa cgcgctggtg ttgaagttgt
    11761 tcgtggcaat gcaggaggcg atccagcagc tgtcgtattc gatgctttgg aaagagcaaa
    11821 gacagagcag gcagatgttt tactagttga tactgcaggc cgactgcaaa ataaagtgaa
    11881 cttgatgaac gaattagaaa aaataaaacg agtaatccag cgggaattac cagaagcacc
    11941 ccaagaaaca cttcttgtat tagatgcaac cactggtcaa aacgcgatgg ttcaagcaaa
    12001 acaattcaaa gaaacaacag atgtgaccgg tcttgttttg acaaaattag atggtacagc
    12061 aaagggcggt atcgtacttg ctatccgtaa tgaattgcat ttaccagtga aattggtcgg
    12121 tcttggtgaa ggaatcgatg acttggagcc gtttgatcca aatgattttg tcgttggatt
    12181 gttcaaagga ttactaaaag aagaataacc catgtctaaa aagcaggagc tgtgacaaaa
    12241 gttgcagctc cttgtttgaa tgattgtttt taacatagct gtgtttttga tgccttcatt
    12301 tttatactta tttgtaagcg gttctcaaac aatttcgttc tttttcttag actttttgat
    12361 tataattaga ttcatatgaa ttggaggtca cttaatgatg aattttaagc aattattatt
    12421 gcatgtcaat gcgcgccctt ttatcgacca agctcgattt ggtatagaac gggaaggaca
    12481 gcgggttgat cttgcaggaa atctagcaaa aaccgatcat ccagcaatct ttggcgatcg
    12541 atcctatcac ccctatatcc aaacagattt tagcgaaaca caaacagaga tgatcacccc
    12601 tgttaccgat tctattcccg aattatttca gtatctcgct gctgtttatg atgtgactgc
    12661 tcgttctata ccgaaagaag agatgatctg gccattaagt atgccacctg ccttaccgga
    12721 aaaagacgaa gagatcatta ttgcaaaatt aaaaaatttc gaagatgtct tgtatcgacg
    12781 ttatttagca aaagaatacg ggaagcgaaa acagatggta agcggtattc atttcaattt
    12841 tgaatttggc gatgagttgc taagaacatt gttcagccat caggaagaat ttcaagattt
    12901 ttccgaattc aaaacagaac tttatttgaa aacagcgaga aactttatgc gctatcgctg
    12961 gatgatcact tatttattcg gtgcttcacc aatgagtgag aagaactact ttttagatga
    13021 atcacatccg caagaacctg ttcgcagtat tcgcaacagt gcgctgggct atacgaatca
    13081 tccaaatgtg aaagtttcct atgcttctat gaaacagtat ttagcagata tcgagcgaat
    13141 gatcgaagaa gggaaacttt cggaggagaa agaattttat acaccgcttc gtttccgagg
    13201 cggaaagaaa gtcgcagatt tagcaacaac aggtgttcgt tatattgaat tgcggaatat
    13261 cgatttgaat ccttatgcaa gattgggtat caatccagag caagttcggt tcttacaatt
    13321 attcctgatg tacatgttat ggacagaaga aaaagaagac tgtgaccaat gggtagcaga
    13381 aggtacgact cgaaataaca aagtagcact agaacaacca tcagatcaaa cagaatttca
    13441 tcaagaaggt agagaaattc ttgaaggaat gaagcaaatg ctggttgaat tggattggtt
    13501 ggattctctt tatttagtag aggaagcatt gactcaaatg gatcatcctg aacaaacgtt
    13561 agcagcgaaa ctctatcaag aagcacagct atcgagtcag caagaagttg ctgtggcatt
    13621 gggacatcaa tattataaag aaagccatga acgtccctat cagttggccg gttttcgcga
    13681 aatggaactt tctactcaga tatttatgtt cgatgccatc caaaaaggtg tccaagtcaa
    13741 agtattagat gagtcggatc aatttttgcg tctgcaattc caagaccatg tagaatatgt
    13801 gaaaaatgca aacatgacaa gtaaggacag ctatatcgtc ccacttatca tggaaaacaa
    13861 aacagtaacg aaaaaagtct taaaagaagc tgggttccgg gtaccaggag gcgcagaatt
    13921 ttcatccatg gaagaagcgg taaaagctta tccgagattt gccgagcagg catttgtgat
    13981 caaacctaaa tcaaccaatt acgggttagg tatcacgatt ttcaaggaag gagctagtct
    14041 ggaagattat caagcaggtt tagccattgc ttttcgtgaa gacagttcgg ttttagtaga
    14101 agaatttatg ccgggaacgg aatatcgttt ctttgtgatc gacggagaag tccaagccat
    14161 catgttgcga gtccctgcta atgttattgg tgacagtatc cgaacagtga aagaacttgt
    14221 tgaagaaaaa aatagtgacc ctttgcgtgg aacgaatcat cgtgcacctt tagaattgat
    14281 ccaattaggt gaattagaac aattgatgtt gaaagaacaa gggttgacga tcgaatcagt
    14341 tccccaagcc aatcaaattg tttatctaag ggagaactca aatatcagta caggtggtga
    14401 ttcgatcgat atgactgatg aattttctga agcttacaaa aaaatcgccg tttctgcggt
    14461 agaagcacta ggagctaaga ttagtggtat cgatttgatt ataccagata aagaaatcga
    14521 tcctacaact gataaaaaag catatgggat catcgaagca aatttcaatc cagccatgca
    14581 tatgcatgtt tatccttttg caggaaaagg cagaagattg acgatgaatg tattgaaatt
    14641 gttatatcct gaagtatttt aaaagaaaaa gcttttgaca gattgtccta tttgatgtca
    14701 atctgatcaa aagctttttt tgcatgtaaa aatttatgtg ttttttcgtt ttaaaataaa
    14761 cgatcccaga agcttttttt cttttttggt ttttcttttt cagctggcga agagttttct
    14821 tctgggtatt ttttttcgat atcaatcaca ggttcattta ctgcttcttt tgctgttaag
    14881 agcaatccga attcctcagg atttgcagtg acgtaatcgt tgactacagt gaaacggcta
    14941 ttgtttttcg ttgctagctg gatatattgg ttttggacag aaaggggtag agccccattc
    15001 aagagtatgg aagcatctgg atgttttttg agttctttta aaaagttttc tttgtttttt
    15061 ggttggatca tttcagcaac agtcatacta agatagcagc gttccctgaa tgtccctaaa
    15121 tatttatgct gttcttcagg gttcactaaa ggtgtaccat aaagtccctt atccaaatgg
    15181 ctttttacat catcttgtgc cataaaaaat accctccttc ttctctttgt ctttcttacg
    15241 attatagcat attcccaata atacctaaaa catatgaaaa agcggttgct tttgttcaac
    15301 gattcactta aaataagaat gaaaatcatt ctcaattaga aaggtggatt ggatgcgcta
    15361 cggacgacta gtagcattat tagttatttt aagtagtatt tcattgttca tcggagtatc
    15421 gaatgtacaa ttgtcagaac tgctccagtt agatgggcaa agcttacaaa tcttgctagt
    15481 gactcgtttg ccacgaacga tttgtttgat ccttgtggga gcgaccagta gtatttgtgg
    15541 gttgatcatg caacatttga cccaaaataa atttgtttca ccaacgactg cgggcacgat
    15601 ggatagtgcg cgtttaggta ttttagtggc tatgattttc ctacctggtg catccttgat
    15661 tgtccgatcc ttgacagcat tttgctttgc ttttgcagga acattgcttt ttttatccct
    15721 tacgcgattg ttcctccaga aaaatcaatt gatgcttccg ttgattggag tcatgttagg
    15781 gaatatcatt ggttcagttg ctactttttt tgcttatcaa ttccagttgg tacaaaatat
    15841 gacttcatgg cttcaaggga attttgcgac tgtgatacga ggaaattatg agttattgta
    15901 tgctgttgtt ccattgttgc tcttggtttg gctatttgct taccgattta cgatcattgg
    15961 tctgggcgaa tcgtttgcag taaatctggg aatagattac cagaagatgc agtttttagg
    16021 cattggctta gtatcattgg ctagtgcagt catgttgatc atggtaggaa gcattccttt
    16081 tatcggagtg atcattccga atcttgtctc tagaatatat ggagatcaag tgcacaaaac
    16141 aattggcgta actgctttag caggtagttt gttcttagta ttttgcgatc ttgtcgctcg
    16201 cgtgttgatt tttccttatg aaataccagt cagtgtgatc gttggaatca tcggtggggc
    16261 tatcttcctt tatcttctga taaggggacg acgatatgct taaaaaaaag ccatctattt
    16321 ttattggtat gcttgttctt gttttatttg ttacaggaat gtacttgacg atccatacat
    16381 atggaaactg gcattttgca tgtgcattga ggagtaagaa aatttttgca tttatccttg
    16441 tctcgctagc tgtcagcagt gccacaatca gctttcagac aattacgcat aaccagtttt
    16501 tgactccttc agtattggga cttgagtcgt tatatgtcct tgtccaaacg gtccttttct
    16561 tctttatcgg aggagtgacg atgcttcagc agacaggtac agtttttttc ttattgaacg
    16621 ttgggctgat ggtcgggctg agtctattgt tatttttctt tcttttgaaa aaaatgagag
    16681 cgaatctttt tttattactg atgtcaggga tgattttagg tacgcttttt ggtagtgtca
    16741 gtaccttttt acaagtcatc atggatccga atgaatatga tttgcttcaa ggcagattat
    16801 ttgcaagctt tggcaatatc cagagtcagt atcttttgcc tgcttctttg atcattatcg
    16861 gctgctggta cctgttattt cgatatcgac atgtgctaga cgttttccat ctcggaaatg
    16921 aacaagcagt aaacctcggg atagacacag atcgttttga atttttgatt ttatgttgtg
    16981 tcagtctttt gacagggaca tcaacggctc ttgtgggtcc agttactttt ttagggttta
    17041 ttgtcgcaaa tatcagttat cgtgtctttt ctacttatcg tcatggtcag ctatttatcg
    17101 gaagctttct ggtagctatg ctgtttctga caggaggaca gctcgcagta gagcagattt
    17161 tcggctggaa tacaacagtg agtgtcgtcg ttgaatttgt cggaggaatt tattttgtca
    17221 caaaattgat tcgcgaaagg aagagtatcc tatgattgat ttacaaaagg tcaataaact
    17281 gtaccatgga aaatcagtgg ttaaagatgt acagttaacg attcctgaaa agaaattgac
    17341 tgcttttatc ggtccaaatg gtgctggaaa gtcaacggtg ttgtcgatga tcagtcgttt
    17401 gataccaaaa gatacaggtg aaatctattt agaccataac gaagtaaagg cttggaaatc
    17461 caatgaatta gccaaaaaat tatcgatcct acgacaatcg aatgaaataa atgtccaaat
    17521 caccgtgaaa gaattagtag aatttggacg atttccttat acgaaaggaa aattgacgac
    17581 aaaagacgag gaaatcgttc aggaatcact ggaacacctt gggttagaag aaatagcgga
    17641 tcaatcgatt gacactcttt caggcggtca actccaacgt gcgtatattg caatggtgct
    17701 agcgcaagac acggattata ttttactgga tgaacctttg aacaatctag atatgaattt
    17761 tgcggttcaa atcatgcaga ttttaaaaaa tttagtacaa gaaatcggga aaacaattgt
    17821 tattgttctt catgatatca actttgctgc aagttatgca gacgaaattg ttgcaatgaa
    17881 agatggacag ttatttacgc aaggaacgac ccaagaaatg ataaaaaaag atgtattgga
    17941 tgaattatac gaaatggaca tacggatttg tgagattgaa ggccgcagat tctgtcttta
    18001 tttctaaaac taacattagg agagaagaga tgaaaaaatt agtagtaggt atcttgattc
    18061 tagcatcatt tggtttagca gcatgtggaa atagcaacaa caccagtcag gcagacacaa
    18121 aaaaaagcag cacacaaact gaaactacat tgacgataac agatagtaat ggagaccaaa
    18181 tcgaggtgcc aaataatcca gaaaaagtcg tcgtattcga caatggttct ttagatacga
    18241 tggatgctct tggtgttgga gataaggtag ttggagcagc tacaagcagc cttcctgagt
    18301 atctttcatc ctataaaaaa gtagaatcag caggtggaat aaaagaacca gacttagaaa
    18361 aaattaatca gcttcagcca gatctaatca tcatttctgg ccgacaaaga gattttcaaa
    18421 aagatctaag tcaaattgct ccgaccattt tcctttcttt agatgcgaaa aatccatggg
    18481 aatctttcca gcaaaatgtc actgctttag gggagatctt tggtaagcaa gaagaagcta
    18541 aaactcaatt agaggaatta tctagtgcaa tcgatcagac gaaaaagaaa gcagaagcta
    18601 ctgacaaaaa agcactagtc acacttgtga atgaagggca attatcagcc tacggatctg
    18661 gttctagatt cggtttcatc catgatttat ttggctttga acaagcagat gatcaaatcg
    18721 aagcatccac tcacggtcaa agtgtttctt atgaatatgt gctggaaaaa aatccggata
    18781 tcttatttgt agtagacaga acaaaagcta ttggtggaga cgacagcaaa gatgatattt
    18841 ctgccaatga actggtagca caaacgaatg ctggtaaaaa tcagcagatc atctcccttg
    18901 aaccagatgt ctggtattta agcgggggcg gtttagaatc aatgaaactg atgatcgaag
    18961 atgtgaatca agcatttaaa tagcataaat aacagaggat taaactctca tactatgtaa
    19021 caataatgtt tcttacattc ttaaatagca agagcctatc ctcaaagaag ttatcaaaaa
    19081 ccatccgtaa aatcgtattg atgattttac ggatggtttt tatttatttg agaaatcttt
    19141 tttcaacgtg aattcattat ctacatcata cgggttgatt ctagaatggg gagtggcaaa
    19201 aaatagtgtg atccattttt tcagcatcaa gatcgcctcc tttgatttga atcaaaggta
    19261 caaagcgaaa cttagataaa acttaaattt agatattttt attttgtacc gatctcttct
    19321 ttaaacgatg ttccatctgc tgcttctcgg caataagtcc actagactag aaaatatgag
    19381 aagctatttt ctgtccagcg ttcttattac tcaaagcaaa tcgcagttgt cacaatctct
    19441 tctaaacgat gttccatctg ctgcttctcg gcaataagcc cactagacta gaaaatatga
    19501 gaagctattt tctgtccagc gttcttatta ctcaaagcaa atcgcagttg tcacaatctc
    19561 ttctaaacaa tgttcaatca gctgaccttc ggtattagct caaagcactg atttcacaac
    19621 ctctatttag gcatgtgtct cagcaagcgg ttgatagttg atgatctgca tttttcgata
    19681 aaaatcggcg ggaagcttac tccaccgttt taataattga tagaaataac tctcttctgc
    19741 tttgatttta tactcgctta atatcaatcc aaaactattc gttttttttg aagctaattt
    19801 tttataaata tccaatgctt tggttttatc ttcattgaca aagagtgttt ttacttcaat
    19861 ataagaaccc tcgatatttt gtgtttcttc gataaaatag atgtttttta tatattcctt
    19921 catagtgatt cctccaaaga agtcttttat ttgataagtt tactctaccg aagagttgtt
    19981 aataatccgt gaacaaaaag taattctttt tccaatagtt gttaaaggtt ttttattttt
    20041 tatagtattt tttgttattt aagaaaggaa taaggttaaa caaataacag gttgcttttt
    20101 gggtcttttt ccgttcaaga aactgaacga agaaaaggga aagttgtctc ttttgtgata
    20161 agagaaatca taataaacga atgacttact ttccaataaa ttgagaacag tcagaaccag
    20221 acaaattagt tgcatctgat gaataaagtg attattctat gacatgaggt gatacagatg
    20281 aaattagaag tgacaaaaga agcacaagaa gtattgaaga ataaattaga agcagacgat
    20341 caacttttat tagatatcga aaacggtgac ggtccatttg cagaaagcca agtcagttgc
    20401 caattggata cggcatttcg attgatcatt gtcaaaaaag atacacaaac agatctaagt
    20461 ctttattctg tagtggctga tacaccagtt ggaccaatca aaatcaaaga cagtgcaata
    20521 ctttacatgg atgatccatc cacacttgct ttagaaccaa cttataatag tttacaatta
    20581 aaaggaccaa gcggcttgag aaaaggcaac ttgcaagttg ttcgaaaaga ctagagggat
    20641 aaaatgggaa aattggcgat catcagcgac ctacatgtgg acatcaataa attcggcgaa
    20701 tcagagctga tgcagctttg gcaagttttg caaaagcaca agatcactcg gcttcatcta
    20761 gccggagata cggctaataa agtagaccgt tgcctagctg ttgtcgaatt tttcgtcaat
    20821 aaaggattat ctacgacatt tcatttcggc aatcacgagt tggcagatgt gcccaatgaa
    20881 cagatgatgg accattttcc agacccacat tttttaaatg aacggtatat tgctttaaat
    20941 gagcagaccg ttcttttagg gatgaatggc tggtacgact atcagttttc cgaattgaaa
    21001 gattacgagg caatcgtacg aatgaagcgt ttgtattggt atgatcggtt catccagcgt
    21061 gggaaaacag atctcgaggt caatgcagaa atgcttgcag ctgcaaaaaa tttgctagac
    21121 cagctggaag caaaaaaact tgaggtgatt ttatccactc atttcgttcc taaacgggca
    21181 tttatcatgt atcaatctgc tccttatgag cgttggaaca aattaaatgc atttttagga
    21241 tcagaatcat tcggagaatt acttgaccgt tattcaaata tcaaacaagt agtttttggc
    21301 catacgcatc gtcgctttga agataaatcc atcggtcaca cgatttacag ctgcagacct
    21361 tttggttatt attatgaatg gcagctgacc cgagattttg tcttgaaaga acagttgcta
    21421 gacagctatg atcctatgaa attacgtgtc ctcttgcgtg cccatcaccc agcttttcag
    21481 gcctatcaaa cacaacattt gcaagaggaa tttgaacaag cgatgacaat cgttccgtat
    21541 tgagacagca ttagtaatgt gtaaaagaaa ataccttgta aaacataagg ataagtaaga
    21601 gcagtagatc catttatcgt ggatatgctg ctttttttgc ggaaaataat cagcgaatgt
    21661 ttatttgatc ggatgatttg taactcgcag agtactagat gcaagcgtgt attctttttt
    21721 tgaacgataa tcacaaaaaa aaatataata tacatttttt atttaattat aattaatatt
    21781 gattgaagtc taggcagtca agtcacattt tgatagatag gagggggact tacgaattat
    21841 tttaaaggct atgacagctg tatttctaga ttgtgtttgc atcaagttgc aataaaaact
    21901 agaaatcaaa tcgggctgat aatcatttat aactaatcga tacataggac caaatatagg
    21961 tctatgttct tttatttcat aagtatcgta aaacagaatt ttgctatgga ttaatccaat
    22021 cgactgatat gtttagtaaa taaagaaaga ggaatgtcaa tgatgaaaaa agaaatcgta
    22081 ttaacagcag caatgatgtt attcagcatg gtagcaagta cgacttttgt ttctgcaact
    22141 gaagtatacc caaaggaata taacacagaa ggaacgatta catttgaagc aggagatgag
    22201 ggtgtaactc ctcctgttga tccagagaat cctgatccca ataaacctgt cgatccatca
    22261 gatcctccaa gccctggaac aggaggggca ttatcgattg attatggatc aaaattcaaa
    22321 tttgggactc aaaaaatttc tactgcggat aaaacgtatt atgcagctgc tgatgtgatg
    22381 aacgatgggt ctagaaaacc gacatatgtc caagtgacag ataggagaag taccttatct
    22441 gggtggaaat taagtgtctc tcaaccagaa cagtttaaaa ctgccagtgg ggatgaacta
    22501 gttggagcac agctgaaatt tacgaaaggg caggctgttt ctttggttga tccaacatat
    22561 acgccgcaaa cggtgaattc tgaattaacg ttgacaccgg gaggaaacaa tacgttagca
    22621 attaatgcta aatcaggaac aggtgtgggg acttgggtct atcgatttgg tgcaaatgaa
    22681 aatgaaaatc aagatgcggt acagctttcg gtacccggta aatctgtcaa gttagcgcaa
    22741 caatattcca ctaaattagt ttggacatta gaagatacac ctaacaacta gtttactgaa
    22801 aagaactgtt aaaaataaat acgtagtcat attgtcttgc gaggtaatcg agcaaaaatt
    22861 gtcaaaggct aggctaaatt tgtttggata cgaatagttc atctaggaat ttaacgatca
    22921 ggaggaaaag atatattgaa aaagaggagg atcgtgctca ttttaagcag tctcttagtg
    22981 actaatatct ttccgatcat agggtactct ttggaattga atcagttgga gcatggaaat
    23041 agaagagacg aagaaagcaa actcacttcg acattcgaca gtttaataga atccaaccag
    23101 acacaagaaa gcagtgagga tttaagtatt tcgagtagtg gagagaccac tgacaacgta
    23161 gaagctgagg gttcggacaa acaaggacta gaagatgcac caatttctgc ggttgagctg
    23221 cctgacgatc ccaatattat ttctatcgat aaaatttttc aaacacctat cggtactagc
    23281 acgtccatat tggaaaatgg gaagttactg cagttgaacc cagcagaaag gtctcaaaga
    23341 ggagctattt ggtcaaataa gaagattagt ttactctcgg attttacatt taaatcttat
    23401 ttatacttag gaaatcaacg agatagagcg ggagatggca tgacctttac attgactggc
    23461 gatcctagaa tggctagtaa tcccagtcaa gttattggat cgccaggtat gggaattggc
    23521 tcatattcta ctcgttctgg ccaaccttac attgcgaatg cgctctcgat tgagtttgat
    23581 acgtataaaa atagtggttc ttctgatcga atggatcgag agataactgc tgataataga
    23641 gggcatggac atgtcgcttt tgtcacgcct aaaccaaata ataataatta ttctggagag
    23701 catactggcg tgacggtagc tcctacattt ctttctaatg gcacatggcg cttgttaata
    23761 attaaatggg acgcggagca aagaagtctt acttatgatt tggaaggtgt cggtcactct
    23821 tctcggatta tacctgatct cattaggcag tttggttcta cagaagtaaa ttgggggttt
    23881 acttcttcaa caggtacttt ttttcaagaa aatgctgtcg ctgtcacgcg acttccatcg
    23941 aatgtcacat caacagctga tgtaaaagtc aacgatagtg attatggggc catcgctgaa
    24001 gctggaggaa acgataagat aactatcaga aatacattga aggtcgaggg ggattttctt
    24061 gaatataaga aaatgcagac tagtatcgac ctcccacaag aattaactat tcccgagcat
    24121 atcttgatca atggaaatca aattgatgcc aaagatatct tgataaccgg aaatcgagtc
    24181 acgctaaatc tgacagatta ttttattgct ggagatacgt taataatcga attggagaca
    24241 actttgcagg aagtaattcc tgaaaaagta ctcactatgc attttgaata ccttgaaaac
    24301 gggaatttga ttgagcaatc gaatgaaata aacctaaaga tcgcaaagct gaaggagata
    24361 gatatccatg tttattataa agataaagac agtcagattg atattgctga aatgaaaaca
    24421 atcactggga aaataggaga gaagtataaa gaaactccta ttgatattga agggtatgtt
    24481 tttgactcgg attcagcgaa tacagaaggc atcttctccg aaaatacaga ggatatctat
    24541 ttttattaca gaaaaggcaa actttatctc tcaactgctc ctcaatatgt caattttgga
    24601 aaacataaaa tatccagtag accattaagt gaattcggac agttttcagg tgggttgaaa
    24661 attatagatg aacgggcttt tggaagttgg aaattacaat tgaaacaaga gaaagttctt
    24721 acaaatggag attttgagct tccagaagct ctttcctttg ttacctctga aaatacaacg
    24781 gttataggga catccgctgt tacgattttc gaaagtgatc aaaagggtga aagcgatttg
    24841 tcctctcttt tagattctac agctcatcgt ggaatcagaa tagatattcc tgtagaaaac
    24901 caaagaatcg gaacctttga ggggaaacta tcttggatac ttgcagatgt cccaggaaat
    24961 taacaggaag agaagaatat aacaaaatta ttgagtttca agaagggcac aggtaccata
    25021 ggctcttgtg tcccacagag acaagatttt gttatagtct tctcttacat atgattaaag
    25081 gagcgatttt cttgagacca aaacaacaat tatttataac actcctagtt attttagggg
    25141 cactttttaa caatatatca gtgtcagcta gtgcatttaa tttttcagtg acgccgataa
    25201 cttcagagaa tcaaatcgac aagagaaaaa cttacttcga tcttcaactt gatccggatc
    25261 aggaagtaga agtaaaagcg gaattgagaa atgatacaga aaaagaagtg aaaatcgata
    25321 tttctgtgaa tagcgcaacc accaattcaa atgtgatggt tgaatacgga aaaaacgaga
    25381 ttgaaaaaga catgagcctg atctttgatc tggtagatta tgtgagctat ccacagatag
    25441 tgacgctaaa acctaaaagt gtccaaaccg ttgcttttca tgtcaggatg ccgaatgagc
    25501 gatttgatgg tgtattggct ggtgggatca cattccaaga aatccagacg gaaaaggacc
    25561 aaacagaaac taaggatcaa agcttatcga ttgagaatga atacacctat attattgctt
    25621 tgttgatgca acagacactg aacgaagttg ctccgaacct tactcttcat gaagttaaac
    25681 cagatcaaat caatgcaaga aatgtcatct tagcaaacgt tcaaaatgat caaaaaacct
    25741 atatcaatca agtagttatt gaaacaaaaa ttacaaaaaa aggacattct gaagttttat
    25801 atcaagagga aaaagaagga ttgcagattg cgccgaatac aaatttttct tttccaactg
    25861 ctttaaatgg tcaaccgcta accccaggtg aatatcacct tacaatgact gttttaggaa
    25921 atgaaaatga aaatggaaaa ttttcgagaa aaaaaggaaa tactacgatc aactatacca
    25981 atcaatgggt atttgaaaaa gattttacaa tagacggaaa agtagccaag gaattgaaca
    26041 cgacggatgt tacgattaaa aaagacaata attggattta tatcttagta gcttcacttt
    26101 tcttattgtt tattttatta tgcatacttt tgtttgtttt gagaaaaaaa gaagagaaag
    26161 aaaagtaagg ttaagataac aaatagtaaa tttttat
//
LOCUS       DAZUMV010000024        26015 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000024 DAZUMV010000000
VERSION     DAZUMV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.024"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..607
                     /locus_tag="ACG8FK_000936"
     CDS             5..607
                     /locus_tag="ACG8FK_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096563.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMVSAP"
     gene            675..869
                     /locus_tag="ACG8FK_000937"
     CDS             675..869
                     /locus_tag="ACG8FK_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096564.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            859..1212
                     /gene="tnpB"
                     /locus_tag="ACG8FK_000938"
     CDS             859..1212
                     /gene="tnpB"
                     /locus_tag="ACG8FK_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS5096565.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            1314..2861
                     /locus_tag="ACG8FK_000939"
     CDS             1314..2861
                     /locus_tag="ACG8FK_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS5096566.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(3124..3249)
                     /locus_tag="ACG8FK_000940"
     CDS             complement(3124..3249)
                     /locus_tag="ACG8FK_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS5096567.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(3383..4276)
                     /locus_tag="ACG8FK_000941"
     CDS             complement(3383..4276)
                     /locus_tag="ACG8FK_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS5096568.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(4458..5384)
                     /locus_tag="ACG8FK_000942"
     CDS             complement(4458..5384)
                     /locus_tag="ACG8FK_000942"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS5096569.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(5469..6230)
                     /gene="pflA"
                     /locus_tag="ACG8FK_000943"
     CDS             complement(5469..6230)
                     /gene="pflA"
                     /locus_tag="ACG8FK_000943"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS5096570.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(6474..8705)
                     /gene="pflB"
                     /locus_tag="ACG8FK_000944"
     CDS             complement(6474..8705)
                     /gene="pflB"
                     /locus_tag="ACG8FK_000944"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS5096571.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(8982..11432)
                     /gene="parC"
                     /locus_tag="ACG8FK_000945"
     CDS             complement(8982..11432)
                     /gene="parC"
                     /locus_tag="ACG8FK_000945"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS5096572.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(11444..13489)
                     /gene="parE"
                     /locus_tag="ACG8FK_000946"
     CDS             complement(11444..13489)
                     /gene="parE"
                     /locus_tag="ACG8FK_000946"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS5096573.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(13655..14089)
                     /locus_tag="ACG8FK_000947"
     CDS             complement(13655..14089)
                     /locus_tag="ACG8FK_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS5096574.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            14216..14854
                     /gene="plsY"
                     /locus_tag="ACG8FK_000948"
     CDS             14216..14854
                     /gene="plsY"
                     /locus_tag="ACG8FK_000948"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS5096575.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(14986..15861)
                     /locus_tag="ACG8FK_000949"
     CDS             complement(14986..15861)
                     /locus_tag="ACG8FK_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS5096576.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(15943..16734)
                     /gene="codY"
                     /locus_tag="ACG8FK_000950"
     CDS             complement(15943..16734)
                     /gene="codY"
                     /locus_tag="ACG8FK_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS5096577.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(16752..18152)
                     /gene="hslU"
                     /locus_tag="ACG8FK_000951"
     CDS             complement(16752..18152)
                     /gene="hslU"
                     /locus_tag="ACG8FK_000951"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS5096578.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(18166..18714)
                     /gene="hslV"
                     /locus_tag="ACG8FK_000952"
     CDS             complement(18166..18714)
                     /gene="hslV"
                     /locus_tag="ACG8FK_000952"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS5096579.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(18751..19656)
                     /gene="xerC"
                     /locus_tag="ACG8FK_000953"
     CDS             complement(18751..19656)
                     /gene="xerC"
                     /locus_tag="ACG8FK_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS5096580.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(19854..21230)
                     /gene="trmFO"
                     /locus_tag="ACG8FK_000954"
     CDS             complement(19854..21230)
                     /gene="trmFO"
                     /locus_tag="ACG8FK_000954"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS5096581.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(21199..23277)
                     /gene="topA"
                     /locus_tag="ACG8FK_000955"
     CDS             complement(21199..23277)
                     /gene="topA"
                     /locus_tag="ACG8FK_000955"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS5096582.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(23399..24256)
                     /gene="dprA"
                     /locus_tag="ACG8FK_000956"
     CDS             complement(23399..24256)
                     /gene="dprA"
                     /locus_tag="ACG8FK_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS5096583.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(24312..25079)
                     /locus_tag="ACG8FK_000957"
     CDS             complement(24312..25079)
                     /locus_tag="ACG8FK_000957"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS5096584.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(25072..25932)
                     /gene="ylqF"
                     /locus_tag="ACG8FK_000958"
     CDS             complement(25072..25932)
                     /gene="ylqF"
                     /locus_tag="ACG8FK_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS5096585.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         6929 a         5535 c         4560 g         8991 t
ORIGIN      
        1 tattatggaa attcaacgta gaaccactaa ttttttacat ttaggctctt tttttgcaac
       61 tttgacgatc ttattttttc tatgttattc ataccttaag acctcacatg cgccttctag
      121 tgcattaggc tggttgcttt tggatcattc tgatatcaca gaagccgaag aaaatccgtt
      181 ttttttgatt ttgtcttctc tttgcggctt actgttttct gtttatttcg gtgcctattt
      241 ttttcatcga catcctggta cgttaaacga agcttcaagc gtattgattg catttaaaac
      301 gttttctttt ttagcaattc ttttttattc gtttaatcac aatacgcttt tcttctttat
      361 catgaatggt ttagtcgtga ttttatccat ttttacttat tttatttttt cactcatttt
      421 tagccaattg aaatgtccgc tcttctttcg attagttcct gtcacctgtt ttatttacag
      481 cttgttttcc ttttttgtcc ttgctttaat gccactgacc aacccttcga cgatccaagt
      541 cgcaggaaat ctgttggata tgctcttcat cgtcagtcta ggtgtattta tggtaagcgc
      601 cccatagaac agtaccttac taacaaatgc acctcaagat atacttgagc tatcaagaag
      661 tctggaggtt ttctatgtat aaattaaatg agattgttcc tgtccaatta ggaaacgaaa
      721 agagtgcacc agaaaaaggt aaacccgcgg ctgccaagtt agcgatgcgc atcaaaagag
      781 agaatggttc tgaagttttg atctataacg gtattaacaa ttatattctt caagctattt
      841 tgaaagaact gttgtccaat gctttatgat ttacttcaaa ccaaacatat ttatattgtg
      901 tgcggaaaaa cagatttgcg aaaaggaatt gatggtcttg ccagtcttat tcaacaagag
      961 tatcaactcg agctttacga agatgccgtt tttttatttt gcgggaaccg tcaagaccgt
     1021 ttcaaacttc tctattggga cggtgatggt tttcttctct gttataagcg gatagaaaac
     1081 ggcaagttaa aatggcctcg gacgaaagac gaagttcgaa ccttaacgaa ccaacaggtt
     1141 cgatggttgt tagaaggatt aagtatcgat caacctcgag ctattttgcc aggaaaaaaa
     1201 ggagtttttt aagctggggg gttaacacaa aaaatgtgtt gaacccctat ttcttgtgcg
     1261 tttcttgtgg tattctgtaa caaaatcagt tcgattcaag gtggtgaaaa attttggaaa
     1321 ctacagatac gttgcttcag ttgcttcaag aagcccataa aaccaatcaa gcacaacaac
     1381 aaaccattca gaatcttaca actgaaatcc aactattgaa tgaaaaggtc aattatttaa
     1441 ccaacaaatt atttggacgc tctaaagaga ctctctttga ggaaacgaat gggcaattga
     1501 atctttttag tgatgaagaa atttctgtaa gtgttccaga agcagctgcc accattattc
     1561 cagtaaaagg acatcaacga gttgtaggaa cgaaagcaga caaaatcaaa cacttaccta
     1621 taacagagaa agaacatctc cttcctttgg aagaacaatt ttgtgaacat tgcggttcac
     1681 agatgaaaga tatcggacgg acaaaagtac gagaagaaat tcgtttccat caggctatgt
     1741 tggattgtct cacccattat caacatacct attgttgtaa aagctgcgaa aaagaaggac
     1801 tttcttcttt taaaaaagcg atcgttccta aacctttgat ttctaatagc ttaggttcca
     1861 atagtttagt ggcggaaacg atccggatga aatttggtca gaaagttcct gcctatcggc
     1921 aagagaacta ttggaaacaa acacatgggt tggatatttc gagagataat atcaccaatt
     1981 ggcatattaa agcagtgcaa aacgcgttag accctttagg agaacgatta agagtctatc
     2041 ttaatcaaga agagattcta cacggggatg aaacaagtta tcgtgtgatt gaaagtgcca
     2101 aaacagatac ctattactgg cagttttgta caggaaaaga cagtcagcat cccatcgttt
     2161 attatcacca cgacgaaagt cgtgctggag atgttccgaa aacttttctc aaggagttta
     2221 cagggtattt acactgtgac ggctatagcg ggtataatgc cgtggaaagt gtacgacttg
     2281 tctattgttt cgcgcatgtt cggcggaaat ttttcgaagc aattccaaaa ggaaagaaaa
     2341 acacggatat tcccgcagct caagcagtca aacaattgga taaatggttt gtcctagaaa
     2401 agaaatggaa agacttttct cctgagaagc gattaagctg tcggcaacaa gaactgcgtc
     2461 cattatttat tgctttttat gagtggatgg caacgataga tcctgttgca aaatccaagt
     2521 tggatgcagc cgttcaatat gcgtgtaaac tacgaagtgg ttttgaacct atttttgaag
     2581 atggtcgttt agaactcaca aataatcgtg cggagcgaaa tatcaaagag ctggtgatag
     2641 gacggaaaaa ttggttgcat tccacaagtc ttgaaggagc acgaacttca ggaatcattc
     2701 tgagtgtata taaaacagca gaacttaacg gattgaatcc tgtaaagtat ctagagtttc
     2761 tatttgataa gataccaaat cttcccgtac tttcagcaga aactttagat caactgttgc
     2821 cttggaacaa ggatgtacaa caacattttt ctcgtaacta aaaacgccaa acattcccga
     2881 ttctattcta taggtcgggt tatttgacgt acaggtactg ttctatgggg cgcttactat
     2941 ttatgtggca aagaaaaaac aaacaaaata aaaagcattt tgaagcctga ctattacgac
     3001 ttcataaatg cttttttagt tttaaacact tcaatctaaa agaataaccc aagattggat
     3061 aaattattaa tgactcgcca ttcgtttctt agtgattctg gattatcaaa tggacttcct
     3121 aatctaatca gctagttctt cccataaaat gccatacttg actgaatcat aatattgatt
     3181 atttaaaaat ctaacttttc ttatcactac ttcctttgtc attccacatt tctcaccatt
     3241 tttttgcatt cctttgtttc cagaccatgt agtgaatcta ataggtggta gataggcaaa
     3301 atactcaaat aattctttta accataaagg aagagaaagg ctcctcctgc aagagtcagt
     3361 ataatttttg cttactgctc tcttaggttt tactcactaa aactgttcca tattctttga
     3421 tataagaaaa tgcatgcggt ataaatttct tgctcagctc agaaaagatt tgtcgtttaa
     3481 gaaaggccat ttcctccatt gtcaatgaag gatccaatgt tggtaattca atctcaaaat
     3541 tgtttttttc gacaataggt aattctaatg tgaatgattc ttcttccttt atagtattcg
     3601 tcacaagtag agactgatgg aaaatactag gccggcgatt tttatattta ttcttacgta
     3661 tcctgtcaac gataacttct atttgatcaa aaaagtacga ttcatttacg tctccaataa
     3721 gttcggctaa ttctttgaat gccttcgtta caggaagctg attcttctgt acaaaaaagt
     3781 aattagccaa agtaagcggg aagtgttcta tagaggaaag tgtgataaaa cggtacttct
     3841 catttcctgc agttgtcctc agcaattctg tctctcggct gtttcttact ccataaaaat
     3901 acgcttcttt ccgttcatct tcgaaacaat acgcccaaat aacttctccc atcgctaatt
     3961 tttgttcatc aacagagagt ctcttcaatt gttcagctat ggtttcagca gccgaagtgg
     4021 cttgttgcaa gtaatcttta ggatcaaata ttggaaagtt caaatgatat cttcgttctt
     4081 ccctcttgat taatcctgca tcgatcaata aatcaagaaa atgggaaaat ttattttgcg
     4141 aaaattcttt tttcaattct cttagaatcg tttcttgttc tcgatgttca tatagataat
     4201 cggtcaatga agaaaaaagc ggatcatcgg tcagtttttg ataaaagttt tccttatttt
     4261 ttgaataata gttcatatat gttctctttt ctatccgttt tattttactt aaaaggcttt
     4321 tacgtaaaaa ggaggaagaa caagagtcat aattgactgt tattctcctc catttcttcg
     4381 gcctttagta tgttctatgt ttctttttat atcaatcgct tatccattca ttttgaatgc
     4441 ttcatactac catttgatta gctaaataca gccgtcaatt gaggtacgac ttgtttttta
     4501 cgtgaaacga cacctttaag caaagcacgg ttgttggtca aagttgtttg gaaagcttct
     4561 tctacttttt cttttccttt accagcaaca agtaattctg aatcactgtc taagatgttt
     4621 gtgacaacta atacgaacag atcgtagcca tttgctttca tttcatcttc cattgctgct
     4681 tctagctctg cttgtcgatc catcacttca ttaaggtcca ctgtattgac ttgagcgata
     4741 cgaacgttgg aactgcccat tgggaaactt tttgcatcca aatcaagaag aaccgcagct
     4801 gatttatcac ttagatttgt tcctgctttc aacatttcaa gtccataagt gtttagatcg
     4861 acttcagcga ttgctgcaag ttcttttgca gctgcttcat cttcaggcgt acatgttggt
     4921 gatttgaaca acaatgtatc tgaaatgata gctgagagca tgattcctgc gatttcttta
     4981 ggaattgtta cttggttttc tttatatagt tttaaaataa tcgtacttgt acatccaact
     5041 ggttctgcac gatagtacaa tggattagct gtttcaaagt ttgcaatacg atggtggtct
     5101 actacagcta aaatatttac ttcgctgata tctgaaatac tttgttggaa ttcattatga
     5161 tccacaagca tgacatctgc attttctgct ttagctgact ctactactcg tggtgcagat
     5221 agctgaaagt ggtctaatgc gtattgtgtt tcttcattag gtgtacctag agcaactgct
     5281 tctgtttctt tacctaattc attttgcaag tgtgaaaacg caatggctgc cccgattgca
     5341 tctgtatctg gattttggtg accaaaaact aaaatttttg acatgtcatt cgctcctttg
     5401 tgatttctcc tatttaataa tagcaaaaaa acggaaagaa gcaatactta tcttctttcc
     5461 gatgcgacct atcgcgttaa atattttgta tactcttctg tatgaagcaa tcgtttagca
     5521 ttttcgacac gttcttttgt aggtggttcg atcccttcta atggatattt caaaccaagt
     5581 gtttcccact tgtattttcc catcgtatga taaggaagaa tctctactct gtctacattt
     5641 gacaaggttt gaataaagtt gtctagtcgg atcaaatatt catcataatc acttcgctca
     5701 ggaactagta catggcgaat ccaaaccggt ttgttgattt ttgacaaata ttgagccatt
     5761 tctaaaatat tttcattgtc ccaatgcgtt agttttttat gctcttcatt atcgatgtgt
     5821 ttgatgtcaa ataacagtaa atctgtatac ttcatcaatt cttgaaaacg tgaaaagaaa
     5881 ggttcttcat acgtaaaagg ttttccgcat gtatccaatg tagtatgcat cttccgctct
     5941 tttgcttttt taaatagttc gatcaaaaaa tcgatctgca atagaggttc tccaccacta
     6001 acagtaatac cacctttatc tccccagtaa ggtctgaagc gttctgcttt atctaaaagt
     6061 tcatctgctg tgtattcttt ccctccgcca atattccaag tatctggatt gtgacaaaat
     6121 tggcaacgca tgcggcatcc ttgcataaac acgacaaagc gcagtccagg tccgtctact
     6181 gaaccgaaag tttcgataga atgaacatag ccgattgttt tttcttccat ttaagttcac
     6241 atcttttctt tgatttcatt ttaagtacaa gttagctgtc gaagcgaagc tttctgaaca
     6301 aaatttttcg caattgccgc ttcgttatgt gtaaaattta cagggacgga acatacaagt
     6361 cctgcccctg taaatcgttt ctttcaaata agcatactga ttattcgatt ttgatgcgat
     6421 ggctattcaa cgtcctttcg acgctaagtc gaaaaacttt tttcacgaac tttttacatt
     6481 ctgtcgtgtg aagttctcat caagacatct aattgttgtt ctcttgtcaa gtcacggaat
     6541 ttaactgcat aaccagatac ccggatcgtc aagttcggat atttcattgg attttccatt
     6601 gcatcacgca ataaatcatt actgaataca ttgacattca aatgataagc tccttttgag
     6661 aagtatccat ctaagacatt acgcaagttt tcgattcttg tatcttgttc ttttccaaga
     6721 cctgaaggat tgattgtttg ggtatttgaa attccatcca atgcacagcc gtaattcatt
     6781 ttagctgttg agttcaggct agctaataac ccactttgtt ccgcattgta acttggattt
     6841 gctcctggcg caagtggttt tccagcttca cggccatcag gagtatttcc tgtattttta
     6901 ccgtaaacaa catttgaagt aatcgtcaat aatgaagttg ttggttttga gttgcggtaa
     6961 gttttatgac gtttgatttg tcccatgaag tattccaaga tccatgaaac taattcatct
     7021 gcacggtcat catcatttcc gtatttaggg aagtcgccat ccacttcaaa atcaactgct
     7081 attccttttt cattacggat tgcttttact tttgcatatt tgatcgctga caagctgtct
     7141 gccgcatgag agatcccagc aatacctgta gcaaatgtgc gttgcaagtc agaatccatc
     7201 aaagccagct gagctgcttc ataagaatat ttatcatgca tataatgaat gatgttcaac
     7261 gtattgacat atagttctgc taaccaatcc atcatattca tgaagttatg tttcacttct
     7321 tcaaagtcta gtatttctga tgtgattgga cgatattctg gtccaacttg ggcgccagtc
     7381 acttcatcga ctccgccatt gatggcatat aatagtgttt tagccaagtt agcacgagcg
     7441 ccaaagaatt gcatgtcttt tccgacaact gttcctgata cacagcaagc aatcgcacaa
     7501 tcatcagaac cccattgttc acgaagctga ttatcatttt cgaattgaat cgaagaactt
     7561 tcgatagcga ttctgctagc atatgttctg aatccttctg gaaggtgttc agaatacaaa
     7621 acagtcaagt ttggttctgg tgaaggtccc atatttgtta atgtatgaag cagacggaaa
     7681 tcattctttg taactaatga acgaccatct aatcccatac ctgcaatgga taaagtagcc
     7741 cagataggga atcctgagaa taattggtta tattcaggtg tacgcgcgaa tttgaccata
     7801 cgtaatttca ttactaagtg gtcgatcaat tcttgtgctt ctttttctgt gattactcca
     7861 ttatccaaat cacgttggat atagatatca aggaatgctg agatacgtcc gattgacatc
     7921 gcagcaccat tttgtgattt gattgctgct aaatatccga agtatagcca ttggacagct
     7981 tcttttgcat ttgcagctgg tttagaaata tcatagccat atttcgcagc catttctttc
     8041 atttgcgcta atgcacgata ttgttcagtg atttcttcac gtaaacgaat cacttcttca
     8101 ctcattgtat gataaccagt catatcatga tcttttttct tttgttccat caaatagtcg
     8161 attccataca atgccacacg acgatagtca ccgatgatac gtccgcgacc gtaagcgtct
     8221 ggaagccctg taatgatttt gtttttacga gcagcacgca tttcagcagt ataagcgtca
     8281 aatacacctt gattatgtgt tttgcgccag tcagtgaaaa tatgtgtcaa ttcatcgttt
     8341 ggtgtatagc cattgctttt tagcgcattg ttagccatat tgattccgcc gaacggcatg
     8401 aatgcttgtt tcaaaggttt gtctgtctgt agaccgacga ttttttccaa atctttatcc
     8461 aaatagcctg catcgtgtga agtaatagtt gatacgattt ctgtgtccat atcatagaca
     8521 ccgttacgct cgaattggat atcatttaac ttttgcaatt catcccataa tttttctgtt
     8581 gcttctgttg ggccttccaa aaagctgtca tcaccgcgat attcagtgta gttattttga
     8641 ataaagtctc tagtgtctac gccttctttc catttggtac ctttaaatcc attccattgc
     8701 tccattgtat ggacctccta aatttctgtt ttatgtaaca gtttttacgt gattagtata
     8761 acacagcacc ctatctttgc aagggttttc ttgtttttta tttaacaatt tttatgtttt
     8821 atttacatat atttaaatca acgttgacat accaacattt aagcattgtg aacactttca
     8881 tctttttttg caaacgtata aggatttatt aaaaatgaat gaaaaagaga cgccacctgt
     8941 attaatacag atggcgtctc ttcattaaaa tactacaaca gttacgactc gatgaaaatc
     9001 tcaggagcat cgataacttc gatgacttct ccaccttttt tctcatccat cgcaaatgat
     9061 ccattcgatg ttcgatcacc tatagggaaa tcagttgggt caatcgttaa ctgctgccct
     9121 ttttgattca tcaaggttat ttcttttgat cttccttccg acatatagat aatacggtgc
     9181 ggattttttt tcagctcacg catgaccgtc actccccgct tcgcacgtga agtttggctg
     9241 atttcttgag caagcatgcg tttaacagct cctcgctgag taacgattac gatcggcgta
     9301 tcaccttctg ctaacacaag cagcccgttg ataatcctat ctccatcttt caaattcata
     9361 gacttcactc cagctgcctt tgctccaaca acgggaacat ctgttaaagg atagcgcaaa
     9421 cccattcctt gatgcgttac taagaaaaca tctaagcctg gctgatcttc tttcagatac
     9481 acagcaatca gttcatcttc ttccgccttt agcttcatac agctgagcgg acggctctta
     9541 taggtgcgcc atggttcgaa ctctgtcatt cgtgtttgct tgatcaatcc attcttgctg
     9601 atgaatacaa atgttttctc tgcttgaatt ttttgatatg ggaaaacagc taaaatcgat
     9661 tcgtctacag acagattgga gattgtttga gaaatgtgct caccagcatc tttccatttc
     9721 aaatcgggta gttcatggac agggcggtag atcacatttg ctttatttgt gatcagaaga
     9781 acatggtcca atgtactcaa ttcacctgag tatagcaagt agtcgccttc tcgcatcccg
     9841 atttcttctg gtttggaagc actataagaa cgaaggcttg ttcgcttcac atagccttct
     9901 cgcgtcacac tgacaattac gtcttcttgt gccactaaga cttgcgtatc aattttgatt
     9961 tcttcgattt cgtcttcgat tgtcgttaga cgagcggaag aataattttt tttcacttct
    10021 cgcaattctt ttttcataac agagaaaagc tctttgtcat ttgaaaggat tttattcaac
    10081 tctgtgattt gcgcaatcaa gctttcggat tccttttgaa gctctgtgat atccgtgttc
    10141 gtcaaacggt ataattgcaa tgtgacaatt gcttctgctt gttcttctgt aaattgaaag
    10201 acgtcgacta aattcttttt ggcgtctttt ttgtctttac tttcacgaat cgttgcgatt
    10261 acttcatcca gaatcgataa agctttcatc aatccttcta cgatatgctg tcttttttga
    10321 gctttggcta aatcgaattg actacgtttt gttatgactt gctttcgatg ctcgatataa
    10381 cttcttaaaa tgtctttcag tccaacttga tgcggagtca tatggtcgat agcgaccata
    10441 ttgaaattgt aattgatctg tagctcagtg tttttgaaca aataattcaa aattccttga
    10501 gcattggcgt cttttttcaa ttcgacgaca atttgcagac ctgttctatc actttcatct
    10561 cgtacttcag ctattccatc gattttctta ttcaaacgga tctcatccat ttttttcacc
    10621 aaggtcgctt tatttacttc atatgggatt tctgtgatca cgatttgctg tttattaccc
    10681 ttcattgcct ctatctttgt tttcgaacga agaataactt ttcctcgtcc tgtttcataa
    10741 gcttttttga tttcatcttt tccttgcaag atccctcctg ttggaaagtc aggtccagga
    10801 atatattcca tcaatttttc aaggctcgct tgtggatgat cgatcatata gattgtgcca
    10861 tcgatcactt cagctaagtt atgagtcggg atttctgttg catatccagc agatatacct
    10921 gtcgatccat tgacgagaag gtttgggtat cgagctggaa ggactgttgg ttctttttct
    10981 gtatcatcaa aattccacac gagatcgact gtttctttct cgatatctgc tagcatctct
    11041 ccacttaact tggataatct ggcttcagta taacgcatcg cagcaggtgg atcaccgtcc
    11101 atactcccgt tattcccgtg catttcgatc agtacttccc gcaacttcca gtcttggctc
    11161 atgcggacca tcgcttcata aatactgatg tcaccatgag gatgataatt ccccatgata
    11221 tttccgacag attttgctga tttacggaat cctttgtcat acgtattgcc atctttgttc
    11281 atggcaaata atatccggcg ttgcactggc ttcaatccat cacggatatc aggcagtgca
    11341 cgttcttgga taatatattt ggaataacgt ccgaagcggt cacccatcac ttcttctaaa
    11401 gttaattctt gtacttcttg tcgattttcc atcaggtgac ctcctattct acttcaaata
    11461 actgattgtt ttcattttta tctgctcgtt cttcatctaa taaatcatta gaaactgaag
    11521 gagagatttc agtatcctct tttttatcta agatactgcc atcttcttcg agcgtaaatt
    11581 gcacgtgatt ttcgatccat tttcttcgag gttctacttt gtcacccatc agtgtggtta
    11641 ctcgacgttc agcttgcgca gcatcatcga tacggacgcg gatcaacgta cgggaagtcg
    11701 gatccatcgt tgtttcccac aattgttccg cattcatctc tccaagtcct ttatatcgct
    11761 gcagcatata tcctttgccg acttttttaa tcattgctgc caattcgtca tctgtccacg
    11821 catattcaat gacttgcttc tttccttgtc ctttggatac tttatataaa ggcggtaacg
    11881 cgatatatac tttcccagct tcaatcagcg gtttcatata acgataaaag aaagtaagca
    11941 gtaatacttg aatatgggcg ccgtctgtat ccgcatcggt catgatgatt actttgtcgt
    12001 aattacagtc ttcgatagaa aactcaggac cgactcctgc accaatcgta tagatcatcg
    12061 tattgatttc ttcatttttc aagatatctt gcattttagc tttttcggta ttgatgactt
    12121 ttcctcgtaa aggtagaatt gcttggaact ttctgtctct tccttgttta gcagatccgc
    12181 cagcagaatc cccttcgact aaatacaatt catttttctt agggtttcgg gattgtgcgg
    12241 gtgtcaattt tcctgaaaga agagattcgc cttttttacg cttcttccca tttcggcttt
    12301 cttctctggc tttacgtgct gcttctctcg cttctctggc tttgattgct ttacggacca
    12361 acatctggct catttcgcta ttttcctgca aataaaagcc catttgttca ctgatgacgt
    12421 tatctactac ggtacgagca actggcgtac caagtttttc ttttgtttgc ccttcaaatt
    12481 gcagaagatt ttcaggtaca cggatagaaa gtacagcagc taacccttct ctgaaatcac
    12541 taccttccag attcttgtcc cgttctttca gcaaaccaac ttttctcgca tattcattat
    12601 aagatttcgt catcgccgct ttcataccag cttcatgggt tcccccatct tttgtccgaa
    12661 cgttattgac aaaagataaa acattttctg aataaccgtc attgtattga taagcgacct
    12721 ctacttcgat accttctttt tctcctgaaa aataaacaac tggtgtcaat gtatcttttt
    12781 cttcattcag ataatcaaca aattctttga ttccttcttc gtaatgaaaa atttccttga
    12841 ctggttcttc tccacgaaga tcagaaagag agattttaac gccttttaga agaaaggcag
    12901 attctcttaa ccgttcggct aaaatttcat atgaaaattt cgttgtactg aatattgtat
    12961 catctggtaa aaaatggaca gtcgtcccgt ttgctttttt tgtttttcct atctttttca
    13021 aagtaccgtc aggtttaccg ccgtttttaa atttctgctg atactctacg ccatctctaa
    13081 cgatcgttac aattagccat ttcgataagg catttaccac actggctcct acaccgtgca
    13141 gtccaccaga agttttgtac cctccttgac caaactttcc gccagcatgc agcacagtaa
    13201 aaatcacttc aactgttggt attccggaag catgcattcc tacaggcatc ccacgtccgc
    13261 tatctgcaac agttatacta ttgtcttcat ggatggtcac atcaatctca ctgccataac
    13321 ctgataatgc ttcgtcaact gcattatcga cgatttcata gactaaatga tgcaagcctc
    13381 ggctgtcagt ggatccaatg tacatccctg gtctttttct aactgcctct aatccttcca
    13441 atacttggat ggacgagtca ttgtattcgt tgtttacttt ttttgccaag gcaaacgctc
    13501 cttcaataat actttgcggc aaaaccgcac cacgattatc atactagaaa aggtcgtgaa
    13561 aaaaaagagg gtagacgaga aatcaatttt aattatctcc taaccgcttt ttcgacattc
    13621 ccctctatcg atcatctctt ctttgtggtc atttttattt ttggttttct ttcaacttac
    13681 caagttcaat cttaatacaa cgatccatta cgatatctgt acgtcctgct tttcttagca
    13741 tctcagctgc ttctttgttt tcaatcccta attgtgccca gaacacttta gcatcagttt
    13801 tcaaaaaatc acctgctatt tcaggtaaaa attcactccg tctaaataca tctacgatat
    13861 cgattggatc atcgatttct gtcaaattcg catagacttt ctcgcctaga atttcttcac
    13921 cagccaataa tggatttact ggtatgattt tatatccatt gtcttgcaat acctgtgcaa
    13981 ccatgtaact tgttcgttct ggtttattgc tcaagcctac gacggcaata ttttttgctt
    14041 cttccaaata gttgaaaatc gtttcttgtg ttggattttt tacagacatt tctgccaccc
    14101 cttgcttttc tttttattcc atctattata cttgtatctt tcattttttc aattgttcct
    14161 gttacctatt catgctaaaa taaaggatag ttttcaactt gaaagagggt aaaaaatgaa
    14221 attgatctta ttacttattt tagcatatct tttaggttct attccttccg gtgtctggat
    14281 tggcaaattg tttttcaaaa aagatatccg acaacacggg agcggtaata ctggaacgac
    14341 caatacattt cgtgtattag gcaaaacagc cgggattgtt gtcctgctta tggatatttt
    14401 aaaaggtaca ttggctactt gtttgccgat gatcttccac ctttctggga ttaaccctct
    14461 ttggtttggg gtatgcgcca ttcttggcca tacatttccg atttttgctg ggttcaaagg
    14521 aggaaaagct gttgcgacaa gtgcaggaat gcttttagga tttaatcctg tattttttgt
    14581 ttattcttgt atcatcttta tcgttagttt attttgtaca agtatggtga gtttgagcag
    14641 tatgatcagt gcagtattga tcactttatc tacgatcgtc cttccttatg tagctcctgt
    14701 gattttggct aagcccaatt ggttactgac gatcattgcc tttttagttt cttcctttat
    14761 cttttatcgg cataaagaca atatcaagcg tatcagaaac catactgaaa gtcgaatccc
    14821 gtttggtttg aatagcagca agaaaaaatc ctgatacttt ttcattccaa agagataaaa
    14881 cgggagtgtg acaaatcgat gtcacactcc cttattactt acaggatagc ttttttacac
    14941 accttggtaa acggtgttcc ctgctaactt ctcggtcaca acctcttact tcactgtgat
    15001 ggaatatttt gtcttgaaga tttcatgggc acctagttga ttgatcccta ctttttccat
    15061 caagtttcca ctactgttca ccgtgtcagc gattccgcac cacggttcga tacaaacgaa
    15121 aggcgcttct tttggataag gagaccagat ccctacataa ggcatatctt tatagcttaa
    15181 agttacgcta tgggaagatt tttcacttcg aatggcgtac gcatttagac cccttgtttc
    15241 aaaaaccaag gcatcttgct caaaaagttc acgattcaaa gccagattcg tatttgtctg
    15301 acctaacgtc ttttgatcca tatcgatgaa tgggcctttc aatggtaggc gtaatcttga
    15361 tttcaatgga gaaaatgcca gatagaaatc ttcaaatgac aatccttctt caagaggtac
    15421 attgaaagca ggatgtccac cgatagaaaa gaacatttct ttttcaccgg tatttgccac
    15481 ttgataatga accgtgatgc cgtctcctcc taattcatag ctgatcacta attcaaaatc
    15541 aaatggatac ttttctaatg tctccggcgt actccttaag atgaaactag ccatttcttg
    15601 tccatgttca atgacttcaa aatccatatc ccgagcaaat ccgtgttgcc ccatttgata
    15661 tgttttccct tgataaacat actgatcatc ttttaaacgt ccaacaaaag ggaataaaac
    15721 cggtgcatgt ctgccccaat acgctggatc accttgccag atatactcta aaccatgttc
    15781 ttttgatttc agactgatca attccgcacc gtgttcagcg atttcggcga tcagctggtc
    15841 attttgaata ataacagtca ttgccattcc tccaacttcg acttactttt taaaatggga
    15901 aggctggaac aaacatttct atctgcccca gtccccttac ttctaataac tgtgttttgc
    15961 taactcgtct ttaaaacggg aattcaatac ttttaaataa gttcccttca tgcctaacga
    16021 acgagattca atgattccag ctgattccaa ttttcgtaaa gcattgacga tcacagaacg
    16081 agtgatgccg atactatcgg caatattaga agcagtcaga cgaccttcat ctccatccaa
    16141 tgcttcaaag atagcttgta cagccttcaa ttcgctataa gataatgtgt tgattgccat
    16201 ttggacagcc gttgcactcc gtacatctgc ttcgatgctt cgagacttct gataaaggat
    16261 ctgcataccg acaaccgtag ctccatattc tgctaagact agatcgtcat ctgcaaacga
    16321 ttgttccata cgagataaga taatcgtacc caaacgttcg ccagctccaa agatcggaac
    16381 gacagtcgtc agaccaaatg gatatttctc tcttgactct accggaaatg cagtaatatc
    16441 actagtaatt gggatattag cttcagtttt catcaactgt gccactgtat cagtataaga
    16501 ttcaggaaat tgtttctcaa caaacattgt tttgattcgt tcattgttca catcgtgacg
    16561 ttcattataa cctagtaata ctccgtcact tcctatgata taggtgttgc tgtcaagaat
    16621 accacctagt gtgacagcca ttttatcgta aggcaaatct gcttgcatgt caaaagtatt
    16681 cttttgctgc aaaagtgtgt ttatcttgcg ggttttcgtt agcaagtctg tcattgtcgt
    16741 cctccgattt tttataaaat atagcgactt aaatcttcat ttgccgcaat tgagccaagt
    16801 ttgtcatcca catatgcttc tgtgatcgtt atttctccca tttgcatatc tggtgcttca
    16861 aataatagat cttctagtag tttttccaaa atggtatgca gtctgcgtgc gccgatatta
    16921 tctgtgtcac ggttgacatc gaaagcgatt tgagcgatcc gttcaattgc ttcttttgta
    16981 aacgtaactg acacattttc tgtaccgatc aacgcgacgt attgttttac taaagcatta
    17041 tttggttcgg tcagaatctt cacaaaatct tcagctgtca aatcatctaa ttctacccgt
    17101 atcgggaagc gtccttgaag ttctggaatt agatcacttg gtttagacaa atggaaagct
    17161 cctgaagcaa tgaataggac gtgatctgtt tggatgatcc cgtattttgt gtttacttgt
    17221 gaaccttcga caattggcaa tatgtctctt tggacgcctt ctctggatac ttctccagat
    17281 tgctgtgatt tagaagtgat cttatcgatt tcatcgataa aaataattcc actttcttcc
    17341 gctaatttga ttgcctcact atggatatca gcatccttga ctaattttgc tgattcttcc
    17401 ttgatcaata actctaaagc ttctttgact gttaccgtcc gttccacttt tttcttcggt
    17461 gtcaaagcac ctagagtttc atttaggtcg atccccattt gctccaatcc attgttcatc
    17521 attggtgcag gtttcttttg gtcttcaact tcaatggtta cttcacgact atccagtaaa
    17581 cccttttgta gttgttccaa gactgtacgg cgattgattc gaatatcttc tgtcacttct
    17641 tcttgcggtt cagcggtatt ttgctgacta gcattaaaaa gattcatcat ttgttcaaat
    17701 tgattagtgg attgtttctg ttcttttttg ataccaggta ccaagatttt taccaaacgt
    17761 ttttctgcat tttttttagc acttgtgtat acacggctgt actgctcttt ttctacgatt
    17821 tggatcgcat tttctactaa atcccgaacc attgattcta catctcttcc gacataaccg
    17881 acttcagtaa attttgttgc ttcgactttg acaaatggtg cattgacgat ttttgccaaa
    17941 cgtcgtgcga tttctgtttt accgacccct gtaggtccga tcatcagcat attttttggt
    18001 gtaatatctt gttgcatttt ttcatctaac tgcaaacggc gatagcggtt acgcaaagca
    18061 acggcaacag atttttttgc tgcgtgctgt cctacgatat attcatctaa ttcacttacg
    18121 atttgtcttg gtgtttgttt taattcatac atatctactc acctcttaca gttcttctac
    18181 gataatatta tgattagtaa agatacagat atctgcggca atatttaacg cattttctgc
    18241 aatttctttc gcactcatgt cgttttgagc atagtttttc attgctcggg ctgcagccaa
    18301 cgcaaaattc cctcctgatc cgatagctaa gataccatca tctggtgtga taacttctcc
    18361 tgtacctgaa acaagaagca tttctttatc attcattacg atcagcatcg cttcaagttt
    18421 ttgcatagat tgctgtgtcc gccattcttg agctaattcg acagctgcgc gttgtagatt
    18481 cccgttatat tcgtttagtt tgccttcaaa tttttcttct aaagtaaacg catctgcaac
    18541 acttccggcg aaacctacaa caacttctcc attgtagata cgtcgtactt tctttgcagt
    18601 gcctttcatg acaacagatt cacccattgt tacttggcca tctccagcca tagcaaattt
    18661 cccgtctttt tcgacggcac agatagtggt tgaatgaaat ggtgattcga ccatgtttgt
    18721 ttcctcctaa atttgttctt ttccttattt ttacgctctt ggatggaaag aacgatagtt
    18781 tttttgtaaa ctttcttttg ttacatgggc atagatctgg gtagtcgata aattggcatg
    18841 tcctagcaat tcttggactg tccgcaaatc tgccccatta tttaataaat gagtagcaaa
    18901 tgtatggcgc agcatgtgag gatggatatc actatccaaa ctgctttttt tgatcatttg
    18961 attcaagaca tattcaattc cagtaggcgt gattggtttt ccatgatgat tcacaaaaac
    19021 gatatcatgc tcttcgtgat attttgtcat caatacctgg cggccttccc tgaaatattc
    19081 ttgcagagca tcttgagcaa aagaacctag aggagcatat ctctctttgt tccctttacc
    19141 atggatcaac atgacctctg aatcaaaatc aatgtctgat accgacaaac tgctacactc
    19201 acttaaacgg atgcctgacc cgtaaaggac ttccagtaaa gcgcggttcc tcaaatccaa
    19261 aggactatct cctgctacgc tgtcaaaaag tgcttgcatt tcattttcat aaaaaaaacg
    19321 tggcagtctc aaatttttct ttttgagatg aacgtaagaa aatggatttt cttttatcac
    19381 ttcttctttg agaagatact gataaaacga acgtaggctg gctatcttgc gactgataga
    19441 atttctgcta tagctaggac gatgatcagc taatgcactc aaatagacac gaaccgtaaa
    19501 atattccgcc ttcagcagat cagcgtctcc agattcctcc aaaaaatgtt tgaaatggag
    19561 aatgtcttct tcatatgctt ctttcgtttt ttctgaatat cctctttcga caatcaaata
    19621 acgcaaaaat ttttgttccc acgtggcttc tgtcattttc tcaccttcct tttgtcccag
    19681 agaataattt agcacagcgt ttataaaaag acaaataaat tgtcagaatt taaccaatat
    19741 ttctaagttt gttcacaatt ggcgcaattc ctcacatttt gtttgagaaa ttcttaagaa
    19801 aaacgcaaga aaaagagcga gactatccgt ctcaccctct tcatggagtt tccttactca
    19861 tttgggattg cagttttttc taattctgtt tttattgcct cattagcttt taaagctcgt
    19921 tgtgcaattg cttcatatct tgcttttttg tctcgaatgc gctctggcag ttcaggaaac
    19981 agaccatagt tggcattcat cggttggaaa tgttttcctt ccgcatgagt aatgtagtaa
    20041 gccatacttc caatagctgt ttcttgtgga aactcgactg gttccatttc ttttgctaag
    20101 cgtgcggcgt tgattccagc aagtagtccg ctcgctgcac tttcaacata gccttctaca
    20161 ccagtcatct gacctgcaaa gaacagatct tcgcgtttcg ttgattggta agtcggtttc
    20221 agcaattctg gcgaattcat aaagctgttt cggtgcatca caccatagcg aacgaattca
    20281 gcattttcta acccaggaat catccggaaa acacgttttt gctcgcccca tttcaagtga
    20341 gtctgaaagc ccacgatgtt gtataaagaa gctgcagcat tgtcttgacg cagctggata
    20401 acagcataag gacgtttgcc agttttagga tcttccaatc caactggttt cataggtccg
    20461 aaaagcatcg ttttgaatcc tcgctgtgcc attacttcaa ttggcataca tccttcaaaa
    20521 tatttttctt tctcaaaaga tttaagttct gccacttcgg cattgattag tgcctcatgg
    20581 aaagctttga attcctcttc tgtcatcgga cagttcaagt aagctgcttc tcctttgtca
    20641 tagcgggact tcaaatatac tttatccata tcgatcgtag ctttgtctac gattggtgca
    20701 gctgcatcat agaaataaaa accatctgaa ccattgaatg ctttgatgct ttctgctaat
    20761 ggatgagaag tcaaaggtcc tgttgctact acggtgattc cttccggtat ttcagtaatt
    20821 tcttcgttta tcactgttac taatggatgc tctttgactt ttcgggtaat tgtttcagaa
    20881 aaagaatcgc ggtcaacagc taaggcacca ccagctggca ctgctgtttc atctgccgaa
    20941 gtgattacta ccgaattcaa tcggcgcatt tcttctttca ataaccctac tgcattagcc
    21001 aaactgtttc ctcttaatga attggaacat actagttcag caaaattact tgtgtgatgc
    21061 gcctcagtcg atttgactgg acgcatttca tataaacgga cagggactcc tgcttgtgcg
    21121 atctgccaag cagcttcact tccggcaagc cctgctccaa caacatttac cattttcaat
    21181 gaaagaacct cttttctatt atttttggat gtcttcttcg taatctccat ttacacagac
    21241 tacttgttta ccgcctttga ctttcttctc gaccagatat ccgtcacatt ttggacaatt
    21301 tctgcccact ggtttatccc aagaagtgaa ttcacattct gggaaacggg aacaaccata
    21361 gaatagtctg tttttcttag attttcgttc aatgacttgt ccttcatgac atactgggca
    21421 agtcacaccg atttctttta cgattggttt cgtattccga caatcaggaa aattactgca
    21481 agcataaaac ttcccatatt tccctaattt tatcaccatc ggatgtccac agacatcaca
    21541 atcgaatcct gctggttcat ctttgatttg aattttttcg attttttctt ccgcattcgt
    21601 cagttctttt tcaaacggtt tatagaaacg atcaaccact ttgacccacg cttccgtgcc
    21661 ttcttcaatt ttatccaaat ctccttccat ttctgctgtg aaatggatat cgacgatttg
    21721 agggaagaat tcacagatta atgaattgac aatttctcct agttcagtcg gctcaaatct
    21781 tttttgtgtc aattttacat agtagcgtct ttggatcgta tcaagtgttg gtgcataagt
    21841 ggaaggtcgc cctaccccgt tttcttctag tgcacgaatc aacgtagcct cactaaaacg
    21901 tgctggtggt tgagtaaaat gttgttttgg ttcaatatca acagattgga ctacatcgcc
    21961 ttcttcaagt tctggaagaa tattttcttt ctcttctttg ccgtcatctc taccttccac
    22021 atacacttgc atgaatcctt tgaatttgat ttttgaaccg ttcgcaatga agatgacacc
    22081 attttgttct aatgtcactt tcattgtatc taaaatagct ggtgtcatct gactagccac
    22141 aaaacgtgac cagatcaatg tataaagttt caattgatct ttatctaaat attgtttgag
    22201 ttcatctggt gtacgaagca cactcgttgg acgcacagct tcatgggcat cttgtgcgcc
    22261 ttgagcattt ttgatctttc ggtgaccatg tctagaaaat tcttttccat acgtatcttc
    22321 aataaatgct gcggcctctg cttttgctgt atcggcaatc cgggtagaat cagtacgcat
    22381 gtatgtaatc aagccgactg ttccttgttt acccaaagtg attccttcat ataattgctg
    22441 tgctaccatc atggtttttc ttgttcgaaa gtttaactta cgggcagctt cttgttgcag
    22501 gctacttgta gtgaaaggat tcgcagggtt tcttttgcgc tctttctttt ctactttttt
    22561 aacttcgaag tctgttccat caattctagc agtgacttct tttacacttt gtgcatctgg
    22621 taatttcttt ttcttaccgt cgattcccca gaaatttgct ttgaatttct tgcgagcttt
    22681 tttaaaatta ccgtcgatgc tccaatattc ttctggaaca aattctcgga tctctttttc
    22741 acggtcaata ataattttta aagcgacaga ttggacacgt cctgcactta accctttctt
    22801 gaccttacgc cagagaatag gactgatcga ataaccaacg attctgtcta aggtacgacg
    22861 agcttgctgt gcatctacta aatctacatc gatcgttctt ggttccttaa aagctgcttt
    22921 gactgcgtct tttgtgattt cattgaagac cacccgattt ttttctttca gatccaaccc
    22981 aagtaggtac gctaaatgcc aagcaatcgc ttctccttct ctatccgggt cacttgccaa
    23041 gtaaactttt tgagcttttt ttgctgcggc tttcaggctt ttgatgacat cgccttttcc
    23101 gcgtatagaa atataatggg gttcataatt gttttcaaaa tcgataccca ttttactttt
    23161 cggtaaatct cttatatggc caacactggc cattacttta tagtttcgtc caagatattt
    23221 ctcaattgtc ttggcttttg caggtgattc tacaatcact aaatatttat acgccattac
    23281 tgaatggact cctttccatt gtcgtgttcc tactattaaa agaggattca tacaaatcgt
    23341 agctaatcat atccattcag aaaacgtttg tcaagaaaag caaatgaaaa gtatggtgtt
    23401 attctttaaa ttctgccaag atatcttcac ccgaaaccgt gcaaactgca ccgtcttgga
    23461 tcaaatgatg acatccggta gaatgttctg ttaaaatatt tcctggaaca gaaaaaactg
    23521 ttcttccaag atccaatgct tgctgtgctg ttatcagaga gccgcttttt ttcttggctt
    23581 caaagacaca tgttccttta gacaaagctg cgataatccg atttctcatt ggaaaacaat
    23641 gctttttcgg gccggattga ggaggatatt cactcaaaag aagatgatca tttgcaatca
    23701 cctcttgcaa gtcacgattt tcagcaggat agacttgctc tatccctgtt ccaatgactg
    23761 cgattgtccg tccaccatga gagattgctg cttggtgacc gtatgtatct atccctttcg
    23821 cgcagccact gacgatcaca tatccagctt ctatcactgg ttttagtaaa taataaactg
    23881 tttttttggc taaagcagaa gtgtgtcttc caccaacaac gcttaacatg ttacaagaaa
    23941 gtaaagatag atcaccatta taaaacagta caattggcgg ataggtcaag tgaagcagct
    24001 gagggggata tttgggatca aagcaagtga taaacgactg tttcgattgg attttgcccc
    24061 agttagcgtg aatttcctgc caacttttgc gaaacagctc ccggtgtttg gttattttac
    24121 ctatcttaat gatgtcttca aatccgattt ctttataagg atactgcatc atccagttag
    24181 caagtgccca ttttccttga ctactgattc cttttgtata agcaagtttc aataaatttt
    24241 cttctatttg atacatcata aaaccgcctt tcgtctctct ttctaataag atacgtaaaa
    24301 gcggctgttt tttattgtaa atattttttt acaggtgcaa aacttttacg atggattggc
    24361 gtaattcctt gtcgttcaag tccttctaag tgttcctttg ttccataacc tgcattatgc
    24421 gagaaaccat atccaggata aagcacgtca tattctttca tcaattggtc tcgatatact
    24481 ttagcaatga tacttgcagc agcaatcgac acagatcgtg catccccttt gattattttt
    24541 tcctgctgaa taggcaaatc caaggtcatc gcatcgatca acaaatagct tggtacaatg
    24601 gacaattttt ctactgcctg gatcatcgct aatttacttg cttcataaat attcacatca
    24661 tcgattgtct gctgatcaac gatcccgatc ccgattgcca gggctttttc ttgaacttgt
    24721 tgataaagaa gttctcgctt tttctctgat aattgtttgg agtcattcag ccctaatatg
    24781 atctcatttt tcggcaagat aactgcagct gccacgacag gtccagctaa tggtcctctt
    24841 cctacttcat caatgccggc aatccattgg tgaccttgtg cgtacccttg cttttcaaag
    24901 caaaacattt cttcatattt ttccttaatt aatgcttcct ttttcaatcg tcgttcaaaa
    24961 gaagccagtg cttgctgcac gcctttgcgt ggatcatttc ttaatgattc tagtgtagga
    25021 tcatctattc tctgtatgct tttcaatcgt tctttgatac tttggatgga ttcagtcatc
    25081 acttttttgc tccatttctt cgcaacgatc cagtgtgtat cgtccaagtt ttccttgacg
    25141 aatatcatgc acgactaatt cgctgccacg atcatagtct tctttatacc cacgttttgc
    25201 tgtaatatcc atcagtaatt ccggtgccaa tttttcttct tgctcgattg ataaaccata
    25261 acgatcgctg attcttcctg aataatatcg ggcaaaaaag tctaatccgt aaatcgctaa
    25321 atcatctaaa tggacaagct ggtctttgat cgcgcctgtc agcgctagtt ttttgccgat
    25381 ttcttcatct tcaaatttgg gccataaaat ccctggtgtg tctaacaatt ccaactcatt
    25441 acctaaacga agccactgct gaccttttgt tacgccaggc ttatttcctg tctgtgcaat
    25501 tttcttgcct actagtcgat tcatcaacgt agatttccca acatttggaa tacctaagac
    25561 cattgcccga atagcgcgag gcttgatccc ccgagcagca tctcttgccc gtttttcttt
    25621 caatgcttct ttagccgcag gtactagacc tttaattcct ttactttctt gtgccgtgat
    25681 agctaatgca agatggcctt gtttacgaaa atattcttgc cacagctttg tctgttgtgg
    25741 atctgccaga tccgctttat tcaataaaac aaggcgaggt ttttgctgaa tgatctgatc
    25801 tagcattgga ttacgagaag ataaaggtaa tcgtgcgtct accaattcaa atacgatgtc
    25861 cacatatttc attttttcag aaacttctcg tcgggctttt gccatatgtc ccggaaacca
    25921 ttggatggtc attgttctct ccattccttt cataccggaa tattccccgg ctttacttgt
    25981 ttttttcttt ttcaatataa attcactttt ttatt
//
LOCUS       DAZUMV010000025        25727 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000025 DAZUMV010000000
VERSION     DAZUMV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.025"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            430..852
                     /locus_tag="ACG8FK_000959"
     CDS             430..852
                     /locus_tag="ACG8FK_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5096586.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            849..1079
                     /locus_tag="ACG8FK_000960"
     CDS             849..1079
                     /locus_tag="ACG8FK_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5096587.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            1577..1777
                     /locus_tag="ACG8FK_000961"
     CDS             1577..1777
                     /locus_tag="ACG8FK_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFS5096588.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            1804..2997
                     /locus_tag="ACG8FK_000962"
     CDS             1804..2997
                     /locus_tag="ACG8FK_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5096589.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCALTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3065..4630)
                     /gene="guaA"
                     /locus_tag="ACG8FK_000963"
     CDS             complement(3065..4630)
                     /gene="guaA"
                     /locus_tag="ACG8FK_000963"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5096590.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(4689..5612)
                     /gene="coaA"
                     /locus_tag="ACG8FK_000964"
     CDS             complement(4689..5612)
                     /gene="coaA"
                     /locus_tag="ACG8FK_000964"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS5096591.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            5808..6413
                     /locus_tag="ACG8FK_000965"
     CDS             5808..6413
                     /locus_tag="ACG8FK_000965"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5096592.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            6721..7737
                     /locus_tag="ACG8FK_000966"
     CDS             6721..7737
                     /locus_tag="ACG8FK_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5096593.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            7878..9179
                     /locus_tag="ACG8FK_000967"
     CDS             7878..9179
                     /locus_tag="ACG8FK_000967"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS5096594.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9225..9887
                     /gene="deoC"
                     /locus_tag="ACG8FK_000968"
     CDS             9225..9887
                     /gene="deoC"
                     /locus_tag="ACG8FK_000968"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS5096595.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            9908..10306
                     /locus_tag="ACG8FK_000969"
     CDS             9908..10306
                     /locus_tag="ACG8FK_000969"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS5096596.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            10372..11454
                     /locus_tag="ACG8FK_000970"
     CDS             10372..11454
                     /locus_tag="ACG8FK_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5096597.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            11666..13228
                     /locus_tag="ACG8FK_000971"
     CDS             11666..13228
                     /locus_tag="ACG8FK_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096598.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13221..14354
                     /locus_tag="ACG8FK_000972"
     CDS             13221..14354
                     /locus_tag="ACG8FK_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096599.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            14354..15310
                     /locus_tag="ACG8FK_000973"
     CDS             14354..15310
                     /locus_tag="ACG8FK_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096600.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            15409..16575
                     /gene="deoB"
                     /locus_tag="ACG8FK_000974"
     CDS             15409..16575
                     /gene="deoB"
                     /locus_tag="ACG8FK_000974"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS5096601.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            16599..17417
                     /locus_tag="ACG8FK_000975"
     CDS             16599..17417
                     /locus_tag="ACG8FK_000975"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5096602.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            17434..18141
                     /gene="deoD"
                     /locus_tag="ACG8FK_000976"
     CDS             17434..18141
                     /gene="deoD"
                     /locus_tag="ACG8FK_000976"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5096603.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(18439..19125)
                     /gene="gpmA"
                     /locus_tag="ACG8FK_000977"
     CDS             complement(18439..19125)
                     /gene="gpmA"
                     /locus_tag="ACG8FK_000977"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5096604.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(19297..19878)
                     /locus_tag="ACG8FK_000978"
     CDS             complement(19297..19878)
                     /locus_tag="ACG8FK_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5096605.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(20011..20694)
                     /gene="rpiA"
                     /locus_tag="ACG8FK_000979"
     CDS             complement(20011..20694)
                     /gene="rpiA"
                     /locus_tag="ACG8FK_000979"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS5096606.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            21013..21426
                     /gene="rpsL"
                     /locus_tag="ACG8FK_000980"
     CDS             21013..21426
                     /gene="rpsL"
                     /locus_tag="ACG8FK_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS5096607.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            21510..21980
                     /gene="rpsG"
                     /locus_tag="ACG8FK_000981"
     CDS             21510..21980
                     /gene="rpsG"
                     /locus_tag="ACG8FK_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS5096608.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            22074..24161
                     /gene="fusA"
                     /locus_tag="ACG8FK_000982"
     CDS             22074..24161
                     /gene="fusA"
                     /locus_tag="ACG8FK_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS5096609.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            24333..25520
                     /gene="tuf"
                     /locus_tag="ACG8FK_000983"
     CDS             24333..25520
                     /gene="tuf"
                     /locus_tag="ACG8FK_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5096610.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         8103 a         4760 c         5504 g         7360 t
ORIGIN      
        1 ttattaatgc gatgaatcca atttcaaata gaaaggtcgc tgattttatt tacctataca
       61 actttataaa atttagtcat actcctagaa agtagataaa attggcgact taatggaggg
      121 aaaagtcgcc atgagaaaga taatctgtca tctagttctc ttaattaagt tcttatcaaa
      181 aaaacttatc cacctgtact ggatagatat gaccatcaat gtggttttgc agattacata
      241 gatagaaaaa tcaaagcata tgaacagaga aaacaatctg tttatgtgct tttttggtca
      301 cttaataaat atcttttagg atttctcttc atttatgcaa caaatccccc gttcgtgtcg
      361 gggtttagta tgaagggaga taaagaagca catgccactc cctttcacga cagaaagggg
      421 gtgaaaagca tgaaaccatc ttcttttcaa gaaaccatag aaaatcagtt tgactatatc
      481 tgcaaacaag ttattgaaga tgaacgaaaa gattacttca aacatctaga cagactatca
      541 gcaaaagaag tctcgttttc tgatatagat aactaccttg tcaactcttt ttctactgtt
      601 gatacgtacg tcactgattt ccaactattt aaattatttg gattaagaat caatgttgaa
      661 agtgatttat taagtgaagc cctgcgtcat ctttcagaga aaaaacggaa cattatttta
      721 cttcattact ttatggatat gagtgatgtg gagattgcgg agttactaaa tttgaatcgt
      781 tcgactgttt atcgtcatcg aataagtgga ctagccatga ttaaagaatt catgaaggag
      841 tgtaaagaat gaaaacagct tatccaaggg ttcccttccc cctcattgta aaggcgacag
      901 atggcgacgt ggaagcaatt aatcagattg ttaaacatta tcgagggtat acctcaaaac
      961 gttcattacg tcgcatgaca gacgaatatg gaaattctca tatggttatt gatgaaaccc
     1021 tacgtggcag aatggaaacg aaactcatct ctaagatttt atcgttcgaa attaaatagt
     1081 cccatttttt cgtggaagcg tgtcgaatcg tatccacgct tcccgatttg ggaggtttgt
     1141 tattccatca aagcatattg tgtgttcagt aggttttgat aggctaacaa gccaaacgtt
     1201 ctttgacaac caaataaagg caatcgaata cgtttcggta agaaaagagc tgatgaacta
     1261 acacgccatg acctacaaaa ttagtgagcg attcatgtta gtaattcgaa agtaatcttt
     1321 agcaggattg ctgccatgac tttcttatcg tgataatgat actcccgtat agccatagtc
     1381 cgagcgttag aagcgtcaca ggcaatgggt acggctacat gagaaccatg cagaagtgaa
     1441 attcctgtga gctttgctag agctgttcga ttgctaagat attcttctct tgtcagtgta
     1501 ttattataaa actatttgat ataattatag gtgaatattg gagttttcct agcctataat
     1561 tgggaaagga gtaataatga taacggtcac aaaaaaggat ttagttgagc taggatatgg
     1621 aacttctttt tcagcagaca tcataaaaaa agcaaaagaa ttgatgatta caaaagggca
     1681 tatctactat caatctcgaa agctagatag agtccctcgt gaagctgttg aagaaattct
     1741 aggaattcgg ttttcaaatg aaaacaggtc tgagtagttt ttgcacagta aggagtaaaa
     1801 actatggtta aagacccaat aaaaaaagca aagaatggaa cctattattt tcgtgcaaat
     1861 ctaggttatg attctaaagg gaaaaaaatt caaaaatatc gtagtggatt cactactaaa
     1921 aaagaagcaa gagaagaata ttcgaaactg ctattgatga aaccagaaga attaagtgag
     1981 aataaggata aaatgacgtt taaacattat atctttgaaa ttttccttcc atggtataag
     2041 acacaggtaa agctcagaac atatgaaaat cgcttaccaa ctattaagaa gcatttctct
     2101 tattttgata aaatggctgt ttccgatatt gagcctattg atgtgcaaaa ttggcaacta
     2161 aaattatcaa agaagtgtaa gtcttcttat gtaagagctg ttcaaggact attttcagtt
     2221 gctatggatc gagcaattgt ccttgggata accaccacaa atccatctaa gattattggc
     2281 aatgtgaaaa agcaaaagtc taaaattgaa ttttggacaa aagaagaatt tgaaaaagta
     2341 atctctctaa tttacaaaga agattactat caacactttt tatttatctc tctgtggttt
     2401 ttatttatga ctggaatgcg aattggtgaa gctactgcca ttcaatggga agatattgat
     2461 tttgactcag gagtactaac catcaacaaa acactctatt acaagaatca atcgaattat
     2521 agatttgttg aaccaaaaac aaaggcgagc gttcgtcata ttgtattaga taaatgcgca
     2581 ttaacttatt tatccgaatg gaaggaagta caacaaaatc taattcagac agactttgta
     2641 atgagctata acggtattcc tacccaaaaa catacactat catatgctat tgaacgctat
     2701 gctaagctgg ctggtatcca tcgaatcaga atccatgggt tacggcattc tcatgcttcc
     2761 ttgttaatca gtatgggaga aaatccactt atcattaaag atcgtctagg acacgaagat
     2821 attgagacga ctttaggaac gtatggacat ctttatccta acagtaattt cgaggtagct
     2881 aacaaattaa atggagccat ttcctttaaa gaagcaaaac aaaattatga tacttcacca
     2941 aagaatcaat ttactgtaga ctacttgcga aacaaagaaa tgaaaaaagt gcaataaaag
     3001 tgcagtaaaa agataagaag agccagaaac ccttggtata agaggatttc tagctctcct
     3061 acaactattc ccactccact gttgctggtg gtttagaagt gatgtcatac acgatacggt
     3121 tgacgtgggc tacttcgttt acgatacgta cagagatttt ttgcaagact tcccatggga
     3181 tacgggcaaa gtcagcggtc attccgtcga ttgaagttac agcacggata ccgactgtgt
     3241 agtcgtaagt acgtccgtca cccataacac cgacagagcg gatccctgga agaacagtga
     3301 aatattgcca gatatcacgg tcaagtccag cagctgcgat ttcttcacgc aagatagcat
     3361 cagattcgcg tacgatttct agcttgtctt cagtgatttc gccaagaaca cgaataccaa
     3421 gaccaggacc agggaatggc tgacgccaaa cgattgaatc aggcatgcct aactgagtcc
     3481 ctaattcacg gacttcgtct ttgaataatg tgtttaatgg ttcgatcaat tcaaattgca
     3541 tatcttctgg cagtccacct acattatggt gagatttgat cgtctgtgct gtttctgtac
     3601 cagattcgat aacgtcagta tacaacgttc cttgtgctag gaaagagacg ccttcttcac
     3661 cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa ttcattccca atgattttac
     3721 gtttttgttc tgggtcagat acacctttca acttgtctaa gaagcgatct cttgcatcga
     3781 ctttgatgat gttcaagccg aattttccgc ctagactttc cattacttgt tccgcttcgc
     3841 ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa ttggtcaccg atcgcttttt
     3901 gtaaaagaac cccaacgaca ctagaatcaa cgccacctga aagaccaagc aagacttttt
     3961 tgtcaccaac ttgttcacgg attttttcta cttgcatatc gataaagtta tccattgtcc
     4021 agttaccttc acagtgacaa acatcaaagg caaagtggcg cagcaattcg attccgtatt
     4081 cggaatgacg tacttctgca tggaattgga taccataaaa attacgtgtt gtgtcttgga
     4141 tagaagcaat cggacaatct ttgcttgttc cgactgtttc aaagccttca ggaactttag
     4201 ttaccaagtc accatggctc atccatactg tttgttgttt tggtgtttct ttaaacaaga
     4261 cagcttgatc gcttgttact tctaacaagg ctttcccata ttcgcggttt ttagcaggct
     4321 ctacggaacc gccaagtttg tacgtgatca attgcatacc atagcaaatc cctaatacag
     4381 gaatacctaa attgaagatc tcaggatcga tcccaaatgc attctcatca tatacactgt
     4441 ttggtccgcc agaaagaatg attccctttg gattcatcgc ttttacttct tctgcagtaa
     4501 tgcggtggct caataattcg gaaaagactc caagatcacg aatacgtctt gtgatcaatt
     4561 ggttatactg actaccgtag tctaatacga taatcttttc aacgttcgtc atatcggcaa
     4621 cgtttgtcaa atttattacc cctatccctt taaattttat ctcaaaatag tcaaactatt
     4681 ttaaaatgct aatattttcg catgaaaatc gaatcgatga tatgattttc agttttatgg
     4741 agaattatat cggctcttcc tcgtgtaggc aagatatatt cctgtaaatt tttcaaattc
     4801 acatttttcc atacatcttt tgccatttta aaggcttctt cacgatctcc gatagcaaaa
     4861 cgataataat aattctctgg attttggaaa gccgtatcca gtaacgcacc aaaacgatcc
     4921 aaataccatt tttcgatcag cacgggatcg gcatcgacat agacagaaaa atcaaaaaag
     4981 tcgctgacgt agatttgctg attcgcaggt aattgaagcg tattgatccc ctcaacgatc
     5041 aagatatcag gctgctggat caattcgtac ttgtctggaa tgatatcata cacatcatgt
     5101 gagtaaacag gtacttttat ctcttcctga ccagatttta cacggttcaa gaaatcgatc
     5161 agcatctcca tatcataact ttcaggaaat cctttacggt ccatgattcc acgctctttt
     5221 aatatacgat ttggataaag aaatccgtca gtcgtgatca gctgaacgtt tcttcgtttg
     5281 aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg tactttttcc aacggctaca
     5341 cttccggcga tcccgataat aaaaggcggt acttctacgt atttatgaag aaataaacct
     5401 ttactcaaga acaaagattc aaattctttc atataaagat aaatcagatg agcaagaggt
     5461 acatagatat ctcgaacatc ctgcatggaa atgcggtcat tcaaactttt gatattatct
     5521 agttcatctt gtgtcaaagg tgcttgccca ttacgataaa attctcgcca ttcttctctt
     5581 gccacacggt agtagttcat tggatcattc atattagcct cctgcgttct cccatcaatg
     5641 gaactatttt aacacaaaat aaacgcaaac cccaccactt aagagagatt gccgatcttt
     5701 ttgtataatc tttataatag aaagaaacgg agtcttattc ttgacttgcg cacggataca
     5761 taaaaggata gactgttgtc atgaaagaaa tgagaaggat ggaagacatg acaaatcatt
     5821 actattctga acgcccagat acggcgcacg attttgaaca atgggctttt gagttaaaag
     5881 gcagaacctt tcaatttgta acagattctg gtgtattttc acgtgaaaca gtggattatg
     5941 gttcacgtgt cctgattgat gctttcgatt gggaagagtt acctgatgaa ggaaagatct
     6001 tagatgtggg atgcgggtat ggaccgattg ggttagcttt ggcatttgcg acacagcggt
     6061 ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct agcgcaagga aacgcaaagc
     6121 gtaaccaaat cgaacatgtt gatatccatc aatcgaatat ctatgaagca gttcatgaag
     6181 aaacctatgc agctatcgta agcaatccgc cgatccgtgc tggaaaaaaa gttgtacatg
     6241 aaattttgac aggcgcatat cctcggttga agaagggcgg tacactgaca attgtgatcc
     6301 agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga agaaacattt ggtaatgcag
     6361 aaatcgtcac aaaagataaa ggatattata tcatcaaaag tgtgaaagaa taactagctg
     6421 aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca tgtacgggta cgtatcagtt
     6481 ctttttttgt tgagaagttt ttaaaaacaa agaactattt tttcttcaaa aagaaatagt
     6541 aggaacagag tcgttgtttt ttacgataaa ataaataaga tacagaactt gtatagaagg
     6601 ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac atactttcct tttttctcta
     6661 ttcttttctt gggggttttg gtacactgat agggaattta gatagaaggg agccaatagg
     6721 atgaagctga ccatcagaga tatcgcagaa atggcaggcg tatcagtaac aactgtctca
     6781 cagattttga ataacaaagg aagcagattt agtgataaga caagaaaaaa agtgttgtcg
     6841 atcgtgaatg aacatcatta taagcccgat tatttcgctt caaatgtgat caatcgacat
     6901 tcaaagacca tcggaatgat cgtccctgat gtcactgatt tcttcttctc caaactgatc
     6961 gaaggtgtag agagctattt gaatcctttg ggctatgtga tcatgttatg caattctcgt
     7021 catagccaag aaaatgagat caagtattta caagagcttt cccaccgctc agtggacgga
     7081 atcttgctgg ccacaccaaa tattttacct gagaaatatg cattagacag cggtttttat
     7141 caaaaaatgc caatcatttt aatcgatcgc ggattgaatc gtcgggataa cggacggctt
     7201 atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc tgtttattga aaatggccat
     7261 acgaagatcg gcatgctgaa ggaaaccact ggatactatc aattagaaga acgtttcaac
     7321 ggatatcgtc atgcgctgaa agatcatggc ctgcctttta atgggaaatt tgttgaacat
     7381 ggagagttga ctgtccaagg tggatatact gcatcaaaaa aattactcaa acataaagat
     7441 atcacggcta ttttttgcgg gaatgatgcc atggcgatcg gatgctatca ggcgatcgat
     7501 gaattgggca aaaaaatccc agaagatatc tcagtcatag gattcgatgg attaaagctg
     7561 tcagaataca tgattccgca attgacgaca gtgcagcagc caagttttga tattggattt
     7621 tatgctgcac gtttcctgat cgatacgatc gaatttcctc aaagaaaagt tccgaacaaa
     7681 gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag tattgacaaa gggctaactc
     7741 ggcggtatat tgcttatgta agcatttaca aaggctagtt agtgcttttt ttatttttag
     7801 taaaccagtt tactaaagtg gtttaccttt cctaaaaata atgtataata ccgaatgaag
     7861 acaaggagga tcaggcaatg cgaatggtgg atctgattga aaaaaagagg gatggaaacg
     7921 agctgtcgaa agaggaaatc gagtacatcg tcacgaacta tacgaatgga aagattccag
     7981 attatcaagt cagtgcatta ttgatggcaa ttttttatca agacatgaca aatgaagaaa
     8041 tcacgaactt aacattggcg attgctaatt ctggtgatgt catcgatttg agttctcttg
     8101 aaggaataaa ggtagataag cattcgacag gtggcgttgg tgacacgacc acattgattt
     8161 tagcccccct agtagctagt gtcggagtga cagtggctaa aatgtctgga cgcggtctag
     8221 gttacacagg tggtacacta gacaagctag aagcaattcc cggatttcag atcgagcttt
     8281 cggatgaaga atttgtacgc attgttaatg aaagtaaagt agcggtaatt ggtcaatcag
     8341 gaaacttggc accggcggac aaaaaattgt atgccttgcg tgacgtcaca gcgaccgttg
     8401 attctcttcc gctgattgcc agttcgatta tgagtaaaaa aatcgcggca ggagcagatg
     8461 caatcgtttt ggatgtgacc actggagacg gtgctttcat gaagaacatc gaagatgcaa
     8521 gacgtctggc aaagaccatg acgagcattg ggaaactggc taaccgagaa acagtggcgg
     8581 ttatctcaga tatgtcagaa cctcttggcg aagctatcgg aaatagtcta gaagttgtcg
     8641 aggcaatcga aacattacaa ggaaatggtc cagaagatct agtagaaatg tgctacgcct
     8701 taggaagcca aatggttgtt cttgctggca aagctaagac aaccgatgaa gcaagaacgt
     8761 tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa attcaaagaa atgatccaaa
     8821 atcaaggggg agacccagcg atcgtggaac atccagaacg aattttgact gcacgctaca
     8881 caatggagct tcctgctaaa caaagcggag ttgtgtcaaa aatcgtggca aatgaattag
     8941 gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa agaagaagat attgatcacg
     9001 ctgttggatt gaaactgcat aaaaaaattg gtgacactgt gacaaaaggt gagtcactat
     9061 tgacgatcta tagtaacgac gaagaaatca cttctgtcat tgaattatta tataaaaata
     9121 ttgaaattgg tgaatcggca atgaagccaa cgttgattca tgacatcatc acagaataaa
     9181 ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa agaaatggaa ctaaaccgaa
     9241 tgatcgacca tactattttg aaagcagacg catctaaaga agctgtgatg caaattatcg
     9301 aagaagcaaa aaaataccat ttttattctg tctgcatcaa tccagcatgg gttgcgctag
     9361 cgaaagaaca attacaaggt gagccagtag ccgtatgtac agtaatcggg tttcctctag
     9421 gagcaaacac acccgagaca aaagcatttg aaacaacaga tgccatcaat aacggtgcag
     9481 atgaagtcga tatggtcatc aatatcggtg ctttgaaatc aaaggatgat caacaagtcc
     9541 aaaaagatat cgaagcagtt gttgaagcag caaaagatcg tgcgcttgtc aaagttatta
     9601 ttgaaacatc gttattagat agagaagaaa tcatccgagc ttgcgaaata gcaaaagctg
     9661 caggcgctga ttttgtcaaa acatcaactg gtttctcaac aggcggagca aaggtagagg
     9721 atgtccgttt gatgcgtgaa actgtaggac cagaaatggg tgtcaaagct tccggcggta
     9781 tccataacga agaagaagca atggcaatga tcgaagctgg tgctacaaga atcggaacaa
     9841 gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg ctattaattt tttcaaagga
     9901 gaagctcatg gcagaactca aacaagaatg gatcaacaca gctattgaag cgttagataa
     9961 agcctatgtt ccttattccc attttccggt aggcgcttgt ctcgtaacgg aatcaggcaa
    10021 gatttatcaa ggcatcaaca ttgagaatgc atcatttgga ttaacgaatt gtgccgagcg
    10081 cacagctttt ttcaaagcag tatctgaagg agaaaaaaca tttacgcatc tagtcgtagc
    10141 ggggcacacc cccgatccta tctctccttg tggtgcatgt cgacaagtaa tggcagaatt
    10201 ctgttcccct gatatgccgg ttacgctagt aggagacaat ggtgtgacga aggcaacgac
    10261 cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat ctataactca tgttcattca
    10321 gtcagctgac tgttgaaaaa taaaacaatt attttaggag gctttacaga aatgaaaaaa
    10381 gcaaaattat ttggtttagg tgctgtcgca ctagcagcag ggttattttt aggtgcatgc
    10441 ggcaacaacg gctctactga ttcatccggt ggcaaaagct catcagacac aacaactgca
    10501 gcattgatca cagacacagg tggtgtagac gaccgttcat tcaaccagtc agcttgggaa
    10561 ggactagaaa aatgggggaa agatcaagga ctttcacgag gaaatgatgg cttccaatat
    10621 ttccaatctt caaatgaatc agattacatc ccaaatattg accaagcatt gaatgctgga
    10681 ttcaaaacga tttttggtat tggttataaa ctaaaaccag cgatccaaga acaagcaaca
    10741 aacaatacag gtacgaactt tgttattatc gatgatgtca tcgatggatt agataacgtt
    10801 gtatctgcaa ctttcaaaga caacgaagct tcttatcttg caggtgttgc agcagcttat
    10861 acgactgaaa caaacgtagt cggctttatc ggcggtgtaa aaggtgaagt aatcgatcgt
    10921 tttgatgctg gattcaaagc cggtgttgat gcaggtgcga aagaattagg aaaagaaatc
    10981 aaagtattga atcaatatgc tggtgacttc tctgctccag ataaaggacg ttcaattgct
    11041 caaggtatgt atgcacaaaa cgcagatatc atcttccatg cttcaggcgg tacaggtaac
    11101 ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg acaaaaaagt ttgggttatc
    11161 ggtgttgacc gcgaccaatc agacgaaggc gaatacacat taaacggcga aaagaaaaac
    11221 ttcacattga catctacact gaaagcagtt ggaacagtag tagaagattt ggcacaaaaa
    11281 tcagcggacg gtaaattccc tggcggcgaa cacactgttt atggattgaa agaagacggt
    11341 gttggtctaa cagaaggaca actttctgac gaagcgaaaa aagcagttga tgaagcaaaa
    11401 gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc ctgaagaaaa ctaaaaccat
    11461 ttctatagcc ttgttagaca catgtttgac aaggctatgg tttgattaga acaagaattg
    11521 ttcatttatc caagctgcct tgtgacagtt tggataaggg aacaactccc tgaagtaatg
    11581 tttgtttcaa aagaagaacg ctcactaagc gtgaatctta gtcaatcgtt gccctagata
    11641 gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat attgtcatag aaatgcagaa
    11701 tattaccaaa gaatttggaa cattcaaggc aaatgacaac atcaatttac aagtaaaggc
    11761 aggagaaatt catgcgttgc ttggagaaaa cggtgctggc aaatctacat tgatgaacgt
    11821 gctttccgga ttattagagc cgacatcagg gaaaattttg atgcgtggga aagaagtaaa
    11881 gatcacaagt ccgaccaaag ccaaccaatt agggattggg atggtccatc agcactttat
    11941 gcttgttgat gcctttactg taacagaaaa catcgtgttg ggaagcgagc ctagtcgcgc
    12001 agggatgctt gaccataaaa aagcacgaaa agagatccaa aaagtttctg aacagtatgg
    12061 attgtcagtc aacccagatg cttacgttcg tgatatttca gttggaatgg aacaacgggt
    12121 agaaatttta aaaacacttt accgaggagc agatgtactg atttttgatg agccgacagc
    12181 tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc atgaaggaat tagtcaaaga
    12241 aggcaagtcg atcattttga ttacgcataa gttagatgaa atcaaagcag tagctgaccg
    12301 ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc aacgttaaag acgtaacctc
    12361 acaacaatta gccgatatga tggttggaag aacggtttca ttcaaaacga tgaaaaaaga
    12421 agcgaagcct caagaagtcg ttttgtctat tgaaaatcta gtggtgaaag aaaatcgtgg
    12481 attagaagcc gtgaaaaact tgaacttaga ggttcgtgct ggcgaagtac ttggtatcgc
    12541 tggaatcgat ggaaacgggc agtcggagtt gatccaagct ttgactggtt tgcgaaaggc
    12601 agaaagcgga catatcaagc taaaagggga agacatcact aataaaaaac cccgaaaaat
    12661 cactgaacat ggtgtaggac acgtgccaga agaccgtcat aaatacgggt tagtcctaga
    12721 tatgacatta tctgaaaata tcgcactaca aacctatcat caaaaacctt acagtaaaaa
    12781 cggcatgctg aattattcag taatgaatga acatgccaga gaattgatcg aagaatatga
    12841 tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta tcaggcggaa atcagcaaaa
    12901 agcaattatc gctcgtgaag tcgaccgaga tcctgatctg ttgattgttg caaatccaac
    12961 tcgtggactg gatgtaggtg cgatcgaatt tattcataaa cgtctgatcg aacaaaggga
    13021 caaatacaaa gcagtgttac tgatcagttt cgaattagaa gaaattttaa atgtttcgga
    13081 tcgtattgct gttatccatg aaggagaaat tgtcggtatc gttgatccga aagaaacatc
    13141 tgaaaatgaa ttaggtctat tgatggcggg ctatacctta gaagaagcac gaaaagagtt
    13201 agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa attccgtaat ttattagtgc
    13261 ctgtactttc agttgtttta ggattgatct taggcgcgat attgatggct gcttttggtt
    13321 ttcagcccat ccaaggttat tcttctatgc tagatgcagc ccttggttcg caaagaggga
    13381 taggtgaaac gttaaggcaa gcaacgccgt tgatttttac agcattaggt ttttctgtag
    13441 ctaactcagc tgggtttttc aatattgggc tttctggaca agcactttgc gggtggatcg
    13501 tcagtgtctg ggtggcactt gcgtttccag atctgccaaa agttgttttg cttccgttat
    13561 gcgtgatctt aggtgcctta gctggtgcga tggcagcggc tgttccaggt ttacttcgag
    13621 cattttttgg aacaagtgaa gtgatcgtaa caatcatgat gaactatatc ttattatacg
    13681 taagcacatt tgctttacat gaagtgatgc ctgaaagcta tcgctcaagt ttggactctt
    13741 ccaatatgat cagtcaaaat gcctcactgc ggttaccatg gttaacacaa gcttttggcg
    13801 gctctcgcgt aaactcagga ttatttttag caatcatcgc gcttattttc gtttggctgc
    13861 tgatgaagaa aacgacttta ggatttgaaa tccgttctgt tggattgaat ccttttgctt
    13921 ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt gtcgatggtc atttctggcg
    13981 ctttggcagg gcttggcggt gtggttgaag gattaggaac gtatcaaaac ttctttgttc
    14041 aaacgacttc gctatctatt ggatttgacg gaatggctgt ttccttgctt ggttctggat
    14101 cagcaatcgg tattctttta tcagcgatct tgttcagtat tttgaagatc ggtggacttg
    14161 gcatgcagac aggagcaggt gttccgtttg agatcgtcaa tgtatcgatt gctttgatta
    14221 tcttctttgt cgggatcaac ttcctgattc gtttcgtcat ggctaaagtc ttccaaggga
    14281 aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc aacgaatcaa aaccatgaag
    14341 ggggcgaact ataatgtcga ctatcgaatt aattgccttg atccttactt ctacactggt
    14401 ttattcaaca ccgctaattc taacgtcact aggcggaact ttttctgaac gaagcgggat
    14461 cgtaaacgta gggctagagg gaatcatggt catgggtgct tttagttcca tcgttttcaa
    14521 cttgacgatg agtccatcac ttggttctat gacaccatgg atcgctattt tagtgggtgg
    14581 cgtcgtaggt atcctatttt ctctgattca cgcagtagca acagtcagtt tgcgtgctga
    14641 ccatattatc agcgggacag taattaatct gatggcacct gctttaggtg ttttcttgat
    14701 caaagcatgg tatggtaaag gtcaaactga taacatcacg cagaatctgg ggtatttttc
    14761 tttcccagga ttagcagata ttccggtcat tggaccaatt ttctttaaaa atacattctt
    14821 acctgcttat tgtgctattt tgattgctgt cctagcttgg gtgatcatct tcaagacgaa
    14881 attcggcttg cgcctccgtt cagttggtga aaatcctcaa gctgccgaca cattaggtat
    14941 caatgtatat gctatgcgtt attcaggcgt actgatttct ggcttgttag gcggtatggg
    15001 cggtgctgtt tttgctcagt cgatttccgg taacttttca gctggtacga tcgttggaca
    15061 agggttcatc tccatggctg ctatgatctt tggtaagtgg aatcctttag gggcaatggg
    15121 tgcctcattg ttctttggtt ttgctcaaag cttgagtatc attggcgcac aattaccagt
    15181 tatttcttcg attccgccag tattgctgca aattgctcca tatttgctga caatcattgt
    15241 gctggttgtt ttcttaggga aagcttctgg tccaaaagca aacgggaaga actatatcaa
    15301 atcaaaataa tcttttagcg aataaaaagg gtgggttacg ggaagtgttt ttcgtctcgc
    15361 cctttttgtt ttaaaggcaa taaaaataaa aagaaagaag gaataatcat gtttaaacgc
    15421 gtacatctga tcgtcatgga ttcagtagga attggagaag caccagatgc cgataagttc
    15481 ggtgatgttg gttctgatac attaggacac attgcgaaag aagcaggatt gaacgttcca
    15541 catttagagc agcttggttt aggaacgatc cgtcctcttg aaggtgtgga atcagtgaaa
    15601 gatcatcaag gctatgcgac gaaattagaa gaagtttctg tagggaaaga tacgatgaca
    15661 ggccactggg aaatcatggg attgaatatc aaaactccat ttcgtgtgtt tcctaatgga
    15721 ttccctgatg atctgatcaa acaaatcgaa gaattttctg gccgtaaagt cgtatgtaac
    15781 aaaccttata gcggaacagc tgttattgac gattacggtg aacaccaaat gaaaacaggc
    15841 gatttaattg tatatacttc tgccgatcct gtccttcaga tcgcggcaca tgaagaaatc
    15901 attcctttag aagaattata tcggatttgc caattcgttc gtgatataac aaaagatgat
    15961 ccgtatatga ttggccgaat cattgcacgt ccgtacgtgg gtgaaccagg agacttcaca
    16021 cgaacaagca atcgccatga ttatgcgttg aatccattcg gacacacagt ccttgattct
    16081 ttgaaagaag caggaaagga cgtgattgct gttggaaaga tcaacgatat tttcaacgga
    16141 caaggaatca ctgaatctgt ccgcacgaaa agcaatatgg atggcgttga ccagctgctg
    16201 aaagttatga aaaacgattt ttcaggaatc agctttacca atttagtaga ttttgatgca
    16261 ttatacggtc atcgtcggga tgtagtcggc tatgcgcatg cgattgaaga atttgactta
    16321 cgaattccag aattgcttga agcgatggaa gaagacgact tgctaatgat tacagccgat
    16381 cacggaaacg atccgacgtt tactggtacc gatcatacgc gagaatatgt accgttattg
    16441 gtttacagta aaaaaatgaa agaacaaggg gcattgcctc aaggatatta ttcagatatt
    16501 tcagcaacga ttgctgagaa ctttagtgtt tcttcgactg aaaatggcga aagcttcttg
    16561 aaattattaa aataatcttt ttaaatagga gaaagcaaat gaccaagtta agcgagatgt
    16621 tacaagaaac gacccagttt atccttgata aaggagtaaa agaaattgat tttggtttga
    16681 ttctaggttc aggattaggt gaattagcag atgaaatcga agatgctgta atgatttctt
    16741 atgatgagat cccgttcttt cctgtttcga cagtagttgg gcatgcagga aagctagttt
    16801 atgggtctct ggcaggcaag aaagtgttag ccatgcaagg gagattccac tactatgaag
    16861 gtcattccat gcaaacagtg acctatccag tacgtgtgat ggccggacta ggggcccatt
    16921 cagtcattgt gactaatgca tgtggtggtg tcaacgaatc atttgtacca ggagatttga
    16981 tgctgattac cgatcatatc aattttactg gtgaaaatcc tttgatcggt ccgaatgaag
    17041 atgcaatggg ccctagattt ccagatatga gtcaagctta tacgcctgac tatcaagcaa
    17101 cagcaaaagc agcagcagaa aaactaggtt tggatcttaa agaaggcgta tatatgggct
    17161 attctggacc aacttatgaa acgcctgcgg agatccgaat gtctcggaca ataggagccg
    17221 atgcagtagg gatgtcgact gttccagaag tcatcgtagc agctcacagc ggattaaaag
    17281 tgttaggaat ctcttgtatt accaacttag cagcaggtat gcaagcgaat ctgagccatg
    17341 aagaagttgt agaaacaact cagagagtca aacaatcttt taaagcattg gttaaagaaa
    17401 cattgacatt attgtaatag gaggaacaat aaaatgagtg tgcatattga agcgaaagaa
    17461 ggcgagatcg ccgataagat tttacttccg ggagatcctt tgcgagctaa atatattgcg
    17521 gagacttttt tggaagatcc agtttgttac aatcaggtac gcggtatgtt aggctttacc
    17581 ggcaaatata aaggagaacg tatttctgtt caaggaacag gaatgggaat gccatcagcc
    17641 accatctatg cccatgaatt gatccagtcg tatggtgtaa agaaattgat tcgagtaggt
    17701 acatgcggtg cattgtcaaa agacgtccat gttcgtgatt tagtactagc acaaggtgca
    17761 gcgaccagtt cttcgatgat cgaaaaaaat ttccaagcct ttcattttcc tccaatcagt
    17821 gatttcaatc tattactgaa agcatatgag atagctaaag aaaagggcta tacggttcat
    17881 gtaggaaatg ttctttcgga agattctttc tacaaagatg atttgacaga gacgttccaa
    17941 ttagctgaac ttggcgtttt aggagtggaa atggaagcag cggctcttta ttacttaggc
    18001 gcgaaatacc atgtgcagac attaagtttg atgaccgtta gcgaccattt gatcaccggt
    18061 gaagaaacaa cagctgcaga acgtcaatct acgttcaatg aaatgatcga agtaggattg
    18121 gaaacagcga tagcagaata agaaggaaat gaaaaagctg ccctgcatta agtaataaga
    18181 acatccaagc aaaaatagct cctcatgttt ttgacttggc tggacttatt attgcaaatg
    18241 taagcttttg aacaccgttt accaagaggt tgtgaatctg ccgtttaact ctgagtcaca
    18301 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgaga
    18361 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
    18421 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
    18481 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
    18541 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
    18601 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
    18661 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
    18721 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
    18781 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
    18841 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
    18901 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
    18961 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
    19021 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
    19081 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
    19141 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
    19201 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
    19261 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
    19321 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
    19381 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
    19441 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
    19501 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
    19561 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
    19621 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
    19681 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
    19741 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
    19801 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
    19861 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
    19921 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
    19981 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
    20041 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
    20101 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
    20161 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
    20221 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
    20281 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
    20341 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
    20401 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
    20461 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
    20521 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
    20581 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
    20641 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
    20701 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
    20761 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
    20821 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
    20881 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
    20941 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
    21001 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
    21061 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
    21121 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
    21181 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
    21241 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
    21301 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
    21361 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
    21421 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
    21481 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
    21541 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
    21601 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
    21661 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
    21721 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
    21781 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
    21841 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
    21901 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
    21961 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
    22021 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
    22081 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
    22141 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
    22201 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
    22261 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
    22321 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
    22381 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
    22441 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
    22501 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
    22561 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
    22621 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
    22681 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
    22741 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
    22801 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
    22861 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
    22921 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
    22981 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
    23041 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
    23101 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
    23161 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
    23221 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
    23281 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
    23341 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
    23401 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
    23461 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
    23521 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
    23581 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
    23641 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
    23701 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
    23761 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
    23821 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
    23881 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
    23941 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
    24001 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
    24061 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
    24121 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
    24181 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
    24241 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
    24301 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
    24361 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
    24421 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
    24481 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
    24541 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
    24601 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
    24661 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
    24721 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
    24781 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
    24841 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
    24901 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
    24961 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
    25021 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
    25081 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
    25141 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
    25201 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
    25261 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
    25321 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
    25381 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
    25441 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
    25501 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
    25561 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
    25621 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
    25681 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaag
//
LOCUS       DAZUMV010000026        25567 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000026 DAZUMV010000000
VERSION     DAZUMV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.026"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..280
                     /locus_tag="ACG8FK_000984"
                     /pseudo
     CDS             152..280
                     /locus_tag="ACG8FK_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            394..633
                     /locus_tag="ACG8FK_000985"
     CDS             394..633
                     /locus_tag="ACG8FK_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096611.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            745..939
                     /locus_tag="ACG8FK_000986"
     CDS             745..939
                     /locus_tag="ACG8FK_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096612.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1025..1696
                     /locus_tag="ACG8FK_000987"
     CDS             1025..1696
                     /locus_tag="ACG8FK_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS5096613.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1749..3725
                     /locus_tag="ACG8FK_000988"
     CDS             1749..3725
                     /locus_tag="ACG8FK_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5096614.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3740..4492
                     /locus_tag="ACG8FK_000989"
     CDS             3740..4492
                     /locus_tag="ACG8FK_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5096615.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4508..5263
                     /locus_tag="ACG8FK_000990"
     CDS             4508..5263
                     /locus_tag="ACG8FK_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5096616.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5276..5536
                     /locus_tag="ACG8FK_000991"
     CDS             5276..5536
                     /locus_tag="ACG8FK_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096617.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5533..7623
                     /locus_tag="ACG8FK_000992"
     CDS             5533..7623
                     /locus_tag="ACG8FK_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS5096618.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7650..8114
                     /locus_tag="ACG8FK_000993"
     CDS             7650..8114
                     /locus_tag="ACG8FK_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096619.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8111..8353
                     /locus_tag="ACG8FK_000994"
     CDS             8111..8353
                     /locus_tag="ACG8FK_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFS5096620.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8371..8673
                     /locus_tag="ACG8FK_000995"
     CDS             8371..8673
                     /locus_tag="ACG8FK_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096621.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8743..10806
                     /locus_tag="ACG8FK_000996"
     CDS             8743..10806
                     /locus_tag="ACG8FK_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096622.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10806..13418
                     /locus_tag="ACG8FK_000997"
     CDS             10806..13418
                     /locus_tag="ACG8FK_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS5096623.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13415..15946
                     /locus_tag="ACG8FK_000998"
     CDS             13415..15946
                     /locus_tag="ACG8FK_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS5096624.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15996..16328
                     /locus_tag="ACG8FK_000999"
     CDS             15996..16328
                     /locus_tag="ACG8FK_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096625.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16329..16922
                     /locus_tag="ACG8FK_001000"
     CDS             16329..16922
                     /locus_tag="ACG8FK_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096626.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17085..19109
                     /locus_tag="ACG8FK_001001"
     CDS             17085..19109
                     /locus_tag="ACG8FK_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFS5096627.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            19109..20308
                     /locus_tag="ACG8FK_001002"
     CDS             19109..20308
                     /locus_tag="ACG8FK_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5096628.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            20324..20968
                     /locus_tag="ACG8FK_001003"
     CDS             20324..20968
                     /locus_tag="ACG8FK_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096629.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            20979..21785
                     /locus_tag="ACG8FK_001004"
     CDS             20979..21785
                     /locus_tag="ACG8FK_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096630.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21807..22037
                     /locus_tag="ACG8FK_001005"
     CDS             21807..22037
                     /locus_tag="ACG8FK_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096631.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22551..22859
                     /locus_tag="ACG8FK_001006"
     CDS             22551..22859
                     /locus_tag="ACG8FK_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096632.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            22861..23283
                     /locus_tag="ACG8FK_001007"
     CDS             22861..23283
                     /locus_tag="ACG8FK_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096633.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            23303..24892
                     /gene="mobP2"
                     /locus_tag="ACG8FK_001008"
     CDS             23303..24892
                     /gene="mobP2"
                     /locus_tag="ACG8FK_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS5096634.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            24904..25239
                     /locus_tag="ACG8FK_001009"
     CDS             24904..25239
                     /locus_tag="ACG8FK_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096635.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            25380..>25567
                     /locus_tag="ACG8FK_001010"
     CDS             25380..>25567
                     /locus_tag="ACG8FK_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096636.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9542 a         3961 c         5257 g         6807 t
ORIGIN      
        1 agatcaaata aacaagaacc gtaggaacta cggggatagc ttggtaaata agaggcacct
       61 ctgttagtaa agtaatacgc tatcaagtat gctctcttcc caagaagctc ccacttcaag
      121 cgtaagaaac cgttaggttt agctaagtgg tgagtagttc acaatgacgc ttacgaatga
      181 taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag
      241 cgttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt
      301 acagataaag gcttagaaga agtcacgacg aataataaaa aggtagtatt accttaggca
      361 ttgagtaagg aattcattta ggaggaaaat tagatggcgc gaccagtttt agaggcgatg
      421 gatatggata ctttgacaaa aggaaggtat gtacagctgt tagctcgtgg ttattcacct
      481 aaagatgtat tcaaatccct gtctaaagga acagatatgg aaaaagaaag attacgaaaa
      541 gaattcgatt attggagaac ccataatgga atcaaggatt tgaaaccagc acggccagta
      601 attaaacgaa agaaaaagat aacccaagac tgaagtgtag agcgtatgtg atcataggct
      661 tttttatttt tgcaaaaaac aaggaaggta ggaaaactca tgaattatga agaaatcgaa
      721 aacagaaaaa aagtgtcgaa ataaatggaa gaaaagttgc taaaaacgat gaaacaaaaa
      781 caccttaaaa gattatctgt catgcaatat atcaatgata tgcagataac aggaaaagag
      841 aaagcttgtt tgttaggttc aatgaaaaac tttgaacagt tgagacgaac ttatgtgaaa
      901 acaagttcta attgtcaact attgttagaa gtctcatgac attcattaat agaattcatt
      961 tttaaaactt tttctatgga aatgagaaaa agattttttg ttttgtctga aagggggtag
     1021 ctggatgaga aaatggttat ttggtttttt aggagtcgct ttaatcgtct tttgcagtgt
     1081 gtttggctat gttagctacc aaaaacatga aggagaggtg tttaagcaaa atatagaaaa
     1141 aaagatgccc gtagatcaaa tcaatgcaca tgcaaagagt tataaggagg atgcaacgaa
     1201 cgtcaataat gatatgtctt tggggcaaat gttgtccata caacaagaag caatcgaaat
     1261 gggcgtcaat aaacaagtat ttgcacaaat tcagattcct gcacttggtt tagcattgcc
     1321 aatttttaaa ggggcaaatc aatacacttt gagtcttgga gcggctactt atttttatga
     1381 agatgctgaa atgggcaaag gaaactatgt cctagcaggt cataacatgg aaatgccagg
     1441 tgttttattt tctgatattc aaaagttgtc attaggggag gtgatggact tagttagtaa
     1501 cgatggcgtc tatcgttaca aggtaacgcg taaatttatc gtacctgagt actttaaatt
     1561 aatcgatggc gttcctgaag agaactcttt cttgtctttg ccaaagaaag gagagaaacc
     1621 cttattaacg ttgtttactt gtgtttatac gtcacaagga aaagaaagat acgtggtaca
     1681 gggagaacta caataacaag gttttaatca acgtgagtcg ttgaaatatg aaatgaggag
     1741 agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg aatgcttatt ggaatgttag
     1801 gaatcattgc aggctgcttt cagggatatc gagcatatgc agaagatgtc acgcaaaaaa
     1861 cgccaccaga gaaggtcaat atcacggttc ataaattaat gtacgatcaa ggaacgcaat
     1921 tgaatgttga tattgacggg atcaaaaacg atggttatac gcacgatcaa tatcctacag
     1981 gtgtaacgaa atacaacaag gcagattatg gtgatgttga gtttacgtta ggaaatatta
     2041 cggatcaagt attaccgaga gaagattctg atctaaccaa cgcgaaagtg gacgagattg
     2101 tacaagatgt agaagacaag ggatctaaca gtgagtacgt caagaatgca accaacaaaa
     2161 ttacttcagc tgtagatgaa aatggtgaga tcacttttac tgatcaacca gcctatgtca
     2221 attccaaagg gaatgtatat gtggtttatg aaagtaaatc cgctgctgga ttagtcacac
     2281 aaaaagcaaa accaatggtt gtgattgcgc caatgacaga taaaacagga tcaggatttt
     2341 taaaaaatat tcatttgtat cctaagaaca ttgtttctaa gttgagtttt gaattaacga
     2401 aatttggtga cgatggcgca gaccaaagca aacaaaccct tttaaaaggg gcaaaatttg
     2461 agttatacaa gggcgaacct ggaaaaggaa caaaattggg tgatcttgta tcggatgatc
     2521 aaggaaagct gactgcgact gatttgacgt taggaaaata ctattttgtg gaagttcctt
     2581 cggaagttgt agtgggatca gatcaagaac caacagcaga tcaatattta ttaggcgcag
     2641 acgctcgcaa cgatgcgcac aataaattaa cctttgaaat tacgaatgat ggcgtgacaa
     2701 gtgacttaaa agcttcatac gtaaactata aagcaccagt gatcgataaa acagtaacaa
     2761 atggaacagg acaagaacat tctttccaaa ttggtgacgc agtaaattat caaggaacga
     2821 ttcatattcc aacagacatt gcaggtggcg cagacggtat cacagtaaac ggtgtgaaaa
     2881 gtgaaacttc tccttacagt gtatttaaat ggggagatac agcaggacag ggactttctt
     2941 atgtcgctgc taaagcaaat atcaaagtaa caaataaaga tggatctgtg gtattaaaag
     3001 aaaatacaga ttataaaatc caaaattcag aaaatggctt tgtgatcgat tttatcgtga
     3061 ataatggcca agtaagtgat actgtagcaa gtttacacgg acaagatctt caaatgactt
     3121 ataacatgta tgtaaatgac agcgcagctg ttgccaatcc attaacaaat agcgtggatt
     3181 tcgtttataa caacaatcca tttaatcagg aagaacacca cgaaaaaacg aaagctgacg
     3241 ttgtgactta tggtgcaaaa ttcttaaaag tcgatagcgg actatttgga acaggaatca
     3301 aagcaacacc gcttgaaggc gcagaatttg ctgctaaaaa cgcagaagga aaatattatg
     3361 gtggattagt tgatacggat aaagacggtg tcaaagaagc agtatgggta gacgatgtag
     3421 caaatgcagc gatcctaaaa tcagataaag aaggtcactt tgaaatcact ggtttaactg
     3481 aaggtgaata ttcactggaa gaaacaaaag cgcctgaaaa ctatcaaaaa ttaacgaaag
     3541 agatctcttt caaagtagac aaagattcat acaaagaaga aaaccgaatc acgatcaaaa
     3601 acaatcaaaa agcttccgta ccaatgaccg gtagcaacgg ttttcaaacg tatgtattaa
     3661 tctcttgctt gttgctagga gcaggggcac tttctgcagt tgtttacttt aagaagaaag
     3721 cctagaagga tcaaaaccaa tgattatcag gaagcctaag aaaaagcgaa tcatgggtaa
     3781 atggatcatt gctttctggt tactttcagc tgtaggtgtc ttacttttga tgcctgctga
     3841 agcgagcgtg gcgaaatacc aacaaaatca gcagattgcc gcaatcgatc gaacaggaac
     3901 ggcagcagaa acggattctt ctttggatgt tgctaagatt gagttaggcg atcctgttgg
     3961 tattcttaca attctaagta tttctttaaa actaccaatt tatgacggaa cgtccgacaa
     4021 aattttagaa aatggtgtag gtattacgga aggaactggt gatattacag gaggaaacgg
     4081 taaaaatcct ttaatcgcag gtcatagtgg actttataaa gataatcttt ttgatgattt
     4141 accttcagta aaaaagggag aaaagtttta tatcaaagtt gatggtgagc agcacgctta
     4201 tcaaatcgat cggattgagg aagtgcaaaa agacgaacta caaagaaact ttgtcactta
     4261 cttagaatca aatcccaatg aagatcgagt aacgctaatg acatgtacgc ctaaggggat
     4321 caacacacac cgttttctag tgtatgggaa gcgagtaacc tttacaaaat cagaattaaa
     4381 agatgaagaa aataagaaac aaaaattatc ttggaaatgg cttttaggaa gtactgtttt
     4441 tctttcagtc atgatcattg gttcgctatt tgtttataaa aagaaaaaat aaaggagggt
     4501 aaccaacatg agaaatagaa aatggctatt tgtttgtgca gccgctcttt tttctttcac
     4561 tgcctacaca cagttgacgt ttgcagacag acaagaaagc acggatagca cagaacaaac
     4621 aatccaaacg tcagatagca gcacgattca aagcagcgat gaaaccgtcg attcgtcaac
     4681 agttgattct tcagaagaac ctaaaattgc tgaagacgat caaatagtca aacagaccat
     4741 tcacgttcaa aagattattt cttcagaaca attagacaac caggggcatg aaaaacaaga
     4801 aggagtaaat ggcgcgaagt tttctgttta tgatgtatcg gacattttac aaaagatgga
     4861 tgttaaggat ctcactactg accaaattga atcccaatta aaaaatcgag tgaaaaaact
     4921 atccagtgat caattgaagc tagtttctaa tgaagaaaca aagacaattg atcaacaaac
     4981 aggtgtcttt gaattctcgg tagaagtaca ggcaaatcag aaacaagctt attatattgt
     5041 caatgaatca agtcctgaaa acatttctaa tagcgaggat attttgctat taacacctgt
     5101 ttctgataaa aacggtgaat tcttaaagga tgtgtggatc tatcctaaat cagaagcttc
     5161 gcaacctaaa gaagaagtaa aaaaaatcgt ttctactggc gtaaagaaaa acttctttga
     5221 gaattgttgg gattttgtta ctcacttgtt ttctagagat taaggaagaa ataaaatgaa
     5281 actgatcaaa ttattattat tcaatttttc ttgtttaacc gcagtctttt tgtactgcgg
     5341 tttattattt tctaaactat ctattttgcc aagctaccaa ctatacctaa tgaggatttt
     5401 aaacatacca gctgcttcca tgccgctaga tattatggaa aagctcatta ggaatccatt
     5461 atttactacg atcaatttga tcgttattgt cggaatttca ctagtcattt ataaaaaaga
     5521 ggggatgaag ggatgaattt taaacactcg tggacaagac ggttaaaata tgtttttccc
     5581 ataatgatga tgttaggtac attgtttggg ttaaaaacag ccaatgtctt tgctgaaaaa
     5641 gttattgtcg atccgtcaaa tccaatccag aacgtaaaga ataattttat tattccaaaa
     5701 gatgttccta atgcaaaaac aaatattact gtaaacggag cgtcaaaagt ttacaaatat
     5761 catgttgatg ccaacaaagg tggttttaca gacgactacg taggattagt ggagggaaac
     5821 aaaaatatac gctatccttc tttccaaaaa gaatacggtg aaacaaacct tgttttagaa
     5881 ggcgtaacaa ccaacaccaa agtcaatatt gactatggaa aaatcggtac gtataacggg
     5941 aaaaaagtca atatcaagct agttctatct aatattcact tatactcgga tacgttgccg
     6001 tggaatctac tagataacaa ctatacaaaa actcattttc gagacgatgg ctataaaaat
     6061 acgaatggtg caatgagtaa atctaaaaaa agaacagtac tttggatctc agacaatctt
     6121 ttctcaggaa ttgtttacca ttcaacacaa atgaatgtgc agctagtagc cacatatgag
     6181 gacggtagcc ccgttcaatt ttcaggagat acatttatca gttttaactc tttgaaccct
     6241 gcaggaggaa aaagcacgga tcttaaagga gaatatgccc actatgacaa aatgaatacg
     6301 acagattggt atgtcagaaa agatacggtt ctttcagaat ttaaatcttt ctacaacaat
     6361 ctgaacgtgg taggtggcca cccaggagga agctctaagc tgacacaagc ggataatgat
     6421 ttcaataatt tgcatgataa attaggagat ccgaagtttg gtcaaggtac tgtgtccttt
     6481 aaaatatctg aagcaaatcc gacgtttgtg attggttctt ctaatgttca aacctggttt
     6541 actttatctt ctgcgacaat ctttagtgtt gttcctgatc aacctgaaaa aacaggggtt
     6601 gataaaaatg gaaataacgt gaacgataaa atgttgcaag taggagacac gattcaatat
     6661 cgaatcaaac agaaggtgaa tcgattagga gtggatttac tagcggttta cgatcgattt
     6721 gagttaatcg ataatttacc aaaagaagta aattacgtcg atgcacacgt tgaaagtggt
     6781 acaaataaaa aattcgatgt atctggtgaa gtgacctatg acaaaacaaa acaccaggta
     6841 aaatatgcag cgaaagctga tacattaaaa aatcgaatga agtataacgg cgaaacttac
     6901 gaattagtga tcaatgtaaa agtcaatgaa ttggccaatc aaaatagtgt agccaaaaat
     6961 cagggaacgt cgatcattaa taaagtagaa aaggatacaa atatcattac cgtttatttc
     7021 cctaaaattc cagtgaaaga agtacaacaa aatggaaaag atgtaaacgg acgaaatgac
     7081 ggtaagaagg gtacgccgac agctccttta aacgcaggat ctgaagtaca atatctagtc
     7141 acacaaaaat ggcacacaaa aggcgtagac gctgcgtcag atcactacaa acaatttagt
     7201 attcaagatc caattgaagc tcgtttgaca tataaagaag gttctgcgca agtaatagat
     7261 aagagtaaag gaaaagatat tacttctgaa ggcacactaa cctatgatag caattcaaga
     7321 acgctgaagt gggaagcttc cgctgacttc ttaagtaaca atcttttaga tggacgagaa
     7381 attcagctga tatttacagc taaaactcca ttacaatcag aaaaaaatat tgataaccaa
     7441 gccgtagtag cagtagataa tgtttctaat aaaacaaatg ttgtgacaat tggggttgat
     7501 cctaacttgc cacaggtcat tgttcctaaa acaggttcta cacatttagt aacgatttca
     7561 gcggtcagct tactattact tgtgttagca acttttagtt atgcggttct gagatacatt
     7621 taaaataaaa aaacgaaaga atgaataaaa tgaaaaagtt aataatagta gttacttcct
     7681 tgattgccat gattgttatt ttagtcggat gtagtaatga aaaaaataaa caaactaatc
     7741 aagataatgg cgtattgaaa tcaggaacta tgtggaaaga agaagtaggt ggtcttgttt
     7801 ataatttgaa aattatagac gagactacct gggaatactc tgagagtgta tggcatccag
     7861 atcctgtaca aataacagtc aagaggcaaa aagactataa aggtttagaa agatacaaaa
     7921 tagtagattc agctggtgtt agggaattta taaacaaatc tgattcttta tttattgttg
     7981 ttccctatga gaagaatggc gtaaaaaaaa taatttttct tggatctagc aaggatgaaa
     8041 agcgaacgaa agagaaattg attcacgatg gatcacaatc caataaatat aagttacaaa
     8101 agactagcga gtgattgcta gtctttttat ttgtcctgaa ggaggaatca cagtggcaaa
     8161 agctaaaacg ccatttgaaa aagccgcgtc taaaatctta gaacgccgcg gtgtatctga
     8221 aacacagtgg aaacaagaag tccttgaaaa agcacaattt gacctattac ggggaaaaga
     8281 caaggactta gaaggatata tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat
     8341 tattaaacac taaaaggagc gaacaatttt atgtttgaat caattacaat gaatttttta
     8401 acacaaccga aagtagtagc tgcggttgat atttttagag cattacaaac tgccgcaact
     8461 agtttaaaaa acaatctgat gatttttgct gctgctgcgg ccgttgcttc cattgttatt
     8521 gggggtcttt tcttcctatt tggacgaaga ggagctgaaa atgggaagat gcacgtaggc
     8581 tatgtattga tgggggtagg agttatttta gcagcagctt ctattgtggt ttcgttattt
     8641 ggattgtttg gacaaacacc tcaaggatta taagaaagta aaaaagtagg agtaagaagt
     8701 agtcgatact ttttactcct tttttatgaa aggagaatac tcatgtactt tacgattgca
     8761 ggcaatacca aaacagataa agagttaata aaaaaagcaa cgaaggaagt aggaaagcgt
     8821 acttcttttt taccgaaaac aattaaatat tctgtcattg aaacggaaga acagctacat
     8881 gtgttaggga aagtactgga acacgaaagt cgatcttctg ttaagttgac tctcacgtta
     8941 catgacacag aaggaaaaat agagaaagct ggtacagaat acgtttttgg cccactggat
     9001 tatcaaattt cagaaggttt ctatcctaca ttgatggatt taatccgcct tgatgtgttt
     9061 aaagaaacgg atgaaactat gagtgtggaa gagtcttatc gaaacgaaaa acttttacaa
     9121 gttttagaag aagagttaga gagcgaccaa agaaaagtga atcaagggag tgaagtcgaa
     9181 gtaaatgaag aagtaccatg ggaacgacca atatttccaa agaaggagtc aaaagaaaaa
     9241 gagacagtct atgttgacga acaagctgat attccgactc agaaatcgat atttgaggaa
     9301 gagaacgatg tgatgggtca attggaggta gatccctttg aagaaacaat ggaggatgaa
     9361 gagacggccg aagtagaagt tgatccagtt gaagcgaatg aagagtcaac atggactgaa
     9421 ggggattctc aggaatatat tgaaccactt atcagtgaag aagtagaaaa aaacgaaata
     9481 gagatacaaa aggcaactgt atttgattat attttggatc aatatccgcc agagcatgaa
     9541 tggatgaaag aaaaaaacaa attattttta aatgagcttt attcgaaata tcaattaccc
     9601 caaacctatc aagcattttt agagagtaaa gaagaattaa tctcacaggg taaaggcaca
     9661 cttttagaac agttagagtc tgtccaatca acaaattggc atgagcttgc acaagaagat
     9721 ttattagcca cctttgaaga acgtcaacaa gaaacagatc aagaaattaa tgagtatgcg
     9781 acggaacagt cgtctaattg ggagaaagca aaagaggaac taaataaaga agaacagcga
     9841 gtgattgaag aagaagtaca aaaaattaaa gagcgatacg aaaagaaacg cgacaatgcg
     9901 tattccttgt gtgtagcaaa gatcaataat tttactcaga cgaatcaaga agcgttatcg
     9961 aaagaaaaag aaaacttgtt aaaagaacgt attaaagaac gaaaagaaga attttaccga
    10021 ggtttgcgta cagataagct aaagattagc aaagaattaa atgaacaact gaataagcta
    10081 tatcaaacaa cgactgaagt tcttatggaa aaatacgaaa agatccagca agaattacaa
    10141 gtgcaaattc caaaatggaa aaaagagtat gaaattcaac aacaagaatt gaaagaacaa
    10201 gaagaaaaac aacggcaaaa agaaaaagag cgcgaacaat tagaactaaa aaaacgtgaa
    10261 caggatcttg cggaacaaca aatggcaata gaaaaacaac ggttaaacca agaaaaggaa
    10321 aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg acaagctcag
    10381 ttaaattatc tagcacaacc tcaaccaacg ccttcagtag ctgctgttca aaccccaatg
    10441 aacaaccaaa caaatctaac tccttcacca cagcgttcct ttaacggatg gatggtggga
    10501 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    10561 aatcaatctt cacaggctga ttctgtggcc acaacgacgt caatcaagga agaagcccaa
    10621 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    10681 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg attctagtaa ggctaaagaa
    10741 ggatcaagcg attcaactgt acaatcaaac acttccgaaa gttcagaatc aaatgaggtg
    10801 aaataatgcg tgttagagaa acaacgaagc gaaaagacaa acgattcaat ttagaaaaga
    10861 ataggtatct ttctcatttc tatcgctata ctccctcagg agaagagcta ccagaaaaac
    10921 atgacaaacg catttcgaaa aagatttttt atagtggact tgtatacctc cctttagagt
    10981 atttagtaac aagagtacca gaagttttca accgattgga aattgttaag ggaagcttac
    11041 aaggaataaa tctgtttcaa ttacaggaaa tgccacaggt ctttcatttt gattcagctg
    11101 gtttttggaa tccatttact cctacaacta gtttgggtac acgtttagca atttcgacag
    11161 tttgtgtggt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtcctctag
    11221 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    11281 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    11341 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    11401 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    11461 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    11521 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    11581 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    11641 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    11701 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    11761 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    11821 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    11881 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    11941 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    12001 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    12061 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    12121 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    12181 taggctataa aggattttgt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    12241 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    12301 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    12361 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggagaaaagt aacttattag
    12421 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    12481 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    12541 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    12601 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
    12661 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
    12721 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
    12781 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
    12841 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
    12901 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggtgaa acgattgtac
    12961 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
    13021 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
    13081 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
    13141 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
    13201 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatctcttg
    13261 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
    13321 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
    13381 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
    13441 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
    13501 gaaagtctta caagattatg gtaattatct gcagtatcaa gatgtattta gttggatttg
    13561 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
    13621 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
    13681 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
    13741 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
    13801 atttatggcc atgatcttga tttttggtgg gagttcttta atcgatgaag ggttaaatat
    13861 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggttcacc
    13921 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acattactag caactgaccc
    13981 gaataaagta gaatccctgc caacagacaa aagaaatgct ttaacagcga aaaattttaa
    14041 aacagcgaat atcaatggga ttattactcc taaacaagct aaagaaatgg cggataaaga
    14101 taagagtggt ttatcagatt tacaaaagga tcggttaaga gcgttacaat accgattgga
    14161 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
    14221 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattatct tagctggtga
    14281 atcttctttg gcggtggcct atttatttat cctatttacg atcgttactt gcattattga
    14341 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggcca
    14401 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
    14461 tttagaatta cgaatctata cattaatgct ttcaggtatc ggtgatttgc atgcggcagg
    14521 aaagatcaat ggattcttgt atgttgttgc tttaattgtg ctaactattg cgctattcaa
    14581 aggctctcaa tcggttacga agattttcgg tgttgatacg agtctgaaaa acggcagtaa
    14641 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
    14701 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
    14761 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
    14821 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
    14881 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
    14941 tgaagatctt acagaaaaag ccaaagacac cttagaagag aaattagacg gcacgagagc
    15001 taaagaattg gctgaagctg tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa
    15061 agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag ttaatgcagc
    15121 agtaaaagct aatacaacaa ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt
    15181 caaagatcac caagaagcgt tgcactctaa tgaaaattcg attgaagaga aaaattccat
    15241 agaagaaaga aattcagaag aaggaaaaaa tggacagtta ccaaaaactg atccagaaca
    15301 atatcgctat aaacctaaca gtgaccttga aaatgagaag cgaatttcag atttatcaaa
    15361 agtaaatcaa gaagacaaag cagaaaatga gggacagtta ccaaaaactg atccagaaca
    15421 atatcggtat agatctgtta actccgaaaa tgaaaaacgc atttcagatt taccaaaacg
    15481 aaatcaagaa gtacaaacgg atgaggattc catcgttctg ccaaatgata atggaatcga
    15541 agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt taccaatgaa
    15601 agatagtcgt ttgaatcaat tcaaggagaa tgatcaaagc tttgttcacc aatcaactgc
    15661 aggtactgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga caactgtttc
    15721 ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag
    15781 agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt tacaagccag caaatgtggc
    15841 atttactgca ccaacaaaaa taaatgtgac aagagaaact aaaaacagga acaatgcatt
    15901 gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg cctacaagtt
    15961 aggcctttta tttatatgaa aaggagaaat caattatgga taaatatata aacatgaaaa
    16021 accctccaag atttagtgga gaatggaagt ttttcgcttt tttcttttta gatctattaa
    16081 cgcttggtgg ctgctttttt tgggcgaaaa tggtcaataa tcttgtccac ttaccactct
    16141 tttttgcact cttgaattat ggcttatgga ttgcgatagc gttagtgatt gtttggcgac
    16201 cctataataa cccaggtcaa cgacaaattt ttgcgattat cgagtctatt ttttttgcgg
    16261 acaagaacca ttatcatgcg ttagatccta accgaaatta tcatggtacg tataaggagg
    16321 atgaataaat gcgagtcaaa ggacaaaatt cagttgaaag gaaatccatt ttgcagtttg
    16381 gaaaaccaga agagcgctct atcttggact tatttccgta taaatcctgt acagaagagg
    16441 gagtcttaat tactagagaa gatcattttc aacgttatta tcgtgtagtt tctacagacg
    16501 tggaaggatt aaatgaacaa gagaaactag aacgaatgaa tcagttgacc accgtcatgc
    16561 gtacgtatgt gccaacaatt aagttagtta gtttgaccac agagacggat ctaacggaac
    16621 aaatcgtgca aaagcggcaa ttattagata aaaatcgtat ggaacagtcc ttaggaagaa
    16681 atctacaacg attaaaaaaa tatgaaaaga aattagtcga agaaatccag gaattgaaac
    16741 gagcagaaca agaacgtcct gacttaagct tctttttcgt cattgaagga aagacaatca
    16801 aggatttaca aaccagaaac cgtcagttaa tgcgatctag tggcatttta gggttaaaac
    16861 cactctacaa aaaagaattg atcaagattc tgtatcgaat gaacaatatg aatgatgagt
    16921 aggaatggag aagagagaat gaaatatccg ttgatatggt tagaaaaaga tgttccgaag
    16981 gaagagacca ctagaaaatt tcctcacact ttaagcctgg gtaagttggg aaaagggcaa
    17041 tcttttaacc aagtaaagaa tcaaacaaga agaggtgact gtacatgtta gaagcaatta
    17101 aaacagcttt ttcaccacaa aatgatcaaa aagtacttga tccattaaag caactaggaa
    17161 atcgggaaat agaaatttta aaatccaaag ggtatgattt agatttttta aaacaagtga
    17221 tgccacaagg tgggttaaat tttgatgaag aatatgtgac gtatggtaat ggttattcac
    17281 gatgtgtgac agtttatcac tacacaaaaa atcctacgct tttttggttg gccaatctca
    17341 tgaacaatcc tcatacagtg gccacactcg atgttagcac agatgaaaag caaaaagtga
    17401 ttgatcaaat caatcgtgcc ttagatgaat taaatgatcg atcagataat gaacgaaaag
    17461 cgactgaccg aaataaaagc gcagaagaat acctggaact agcggaatat gctcgtaaat
    17521 tgacttctaa tggggaaatt tctaagcaag tcaaagtgag agtacatgtt tacggaaaca
    17581 cgattcaaga agtagatgaa cgagcgcagg aactgatcga aaatctaaaa gggatggatt
    17641 atcgtgcggt gacttatctg cataaaacca aacgagattg gcaatcgatg tttaccatgt
    17701 tcgatgaaca agctgaatgg ttcggaaata tcaaagggca atcactacca tcgttaaaca
    17761 ttggcggagg tttcccgttc caccaccaaa cgctaaaaga tccaggaggg acacaatatg
    17821 gggaaaccat gacaggcggc ccatttgtct ggaatgcgac gtataaaaat agtgtacggt
    17881 tgtctactaa taccttgctt ctaggaatga tgggagctgg taaatcgacc gttcttaaaa
    17941 tgatcgcgga agcccattta gctgcaggag actttatttg gggctttgaa aagggaaaag
    18001 actttattcc tttcttaaag gaatataacg gtattatggt gcgtttagac ggatctgacg
    18061 gaatgatcaa tcctttagag atatttgcca cacggacgta tgatgaagca tccagtttat
    18121 atgacgatgg atcagtcaaa gatttgaaga tcaatgaagc agcgtcctat caaacacact
    18181 tagacaaagt ggtctatcag gtacagttag tgagtcctca gctaaaagga acgatgaaag
    18241 cagaatttaa gacctactta aatcgttttt atgaagagta tggaacagta ccacgcggct
    18301 ttacttcctc aaattcgcgt tcaaatacgg agacgcaagt cacaggtaaa gatccagaag
    18361 cttatccgac gttcaaagaa tttttagact ttttaggaca actggaatta ccaggtgctt
    18421 cccaagagaa aaagaatcga aaagaagaaa tggaatccat cgtagaatcc ttatgtgaga
    18481 cgtatggcat gatttttgat gggcatagta cgattcgaca ccttgaccaa caacagttgg
    18541 tcttttttga cattgactct attacaggct tagatcgagg ggtacaacaa tgtcaaatgt
    18601 acatggctat gacgttgatt tggaatcagg cattgattca aggaagaaga caacgacgtt
    18661 taattaaaga aaaacaaatg gaagaacaag acttcaaacg cttcttggcg atgattgatg
    18721 agtgccataa tgcgattaat ccagatttta ttgaaacggt aaattatgtc acaaactttc
    18781 aacgagaaat gcgtaaagta gaagcaatga ctgttttagc tacacaatcc ccacaagaaa
    18841 tggtgccaga aaatatgtct tctgataact tctcgaaact gaagaaagtc tttgaatttt
    18901 cttctacgaa gatctttatg cgtatggatg aatctatttt acgtcatata gaaggattag
    18961 tgggtaaatc aatgacgaac tcagaattag aatccattcc tcaacaaaat caaggagacg
    19021 ctgtgatcaa ttttggctca aaagaaacca ttcgtgttca ttttagtccg aacaaacgtc
    19081 aattaaaatt gtttgatgga ggaaaataat ggaaaaccaa aatgaaagtt taatcaaaca
    19141 gtatgttaag cgtcgtgcaa agcggcgctt atttttgtgg gtgtttggta cttctgcagg
    19201 tttgattact atattgatca ctgtgtttgt gacccttttt ctaatacttg cggcaggttc
    19261 tattgataat tctgattcaa gtagtggtgg cgaagctttt acaggagaat attcggaagg
    19321 cttacccatt tataaagaaa tcaaaggaag aggccctttt tctgatgaga ttgcgcaata
    19381 tgctgtaggt gcagcagtga agtataaatt attaccaagt gtgatccttt cgcaatacgg
    19441 ttatgaatca gcgtttggta cgtcggcgtc cgcaagaaat gatctcaatt attttgggat
    19501 tacgtggttt gatgggtgtc tgtttcctaa aggaactgcg cgtggaattg gtggcattga
    19561 aggtggctgg tacatgaagt ttcccaactc aaaagctgcc ttctcctatt acggctttat
    19621 ggtagccaca caatcaaatt ttaatgcttg tgtcggaaat aaaagccctg gcgcaagctt
    19681 attgatctta ggacgaggag ggtatgccgc cgcaggaatt actgaagaca gtgcctatta
    19741 taaaaattgt atgtctatta ttaatacaaa taaattgatg gagtacgatg agtttgcgat
    19801 taaacactgg agagaaggcg gcggaactgg tggtacgatt acaggaagct ggggcaatcc
    19861 attcccagga agtagcttag ataaaaattc gttcagtgga ggacaacttt ttgggaaaaa
    19921 tccaggagga gaattccgac ctaacgggtt ccatgatgga ctagactttg gttcagtcga
    19981 tcatccagga agtgagattc atgcggttca tggcggaaaa gtcgtgtacg ttggcaatcc
    20041 tggtatttca ggtttgggtg cgtgtgtgat cgtgattaat tacgatggct tgaatatggt
    20101 gtatcaagaa tttgcaaata gtacaggaaa ttctcgagtg aaagtaggcg atcaagtgaa
    20161 agttggtcaa gtaattggga tacgagatac agcacacttg catttaggat ttactcgaat
    20221 ggattggcgt caagcacaag gacatgcatt cattgatgat ggtacatgga tcgatccgtt
    20281 accatttttg aatagcagca aaaaatagat caggagaaaa aatatggaaa ataaaacctt
    20341 aaaaatcgtc ttaacagctg caattagcgt tagtttaatc ggcaatattg cgttgttttg
    20401 gcagcttaaa gaagaacaag agacaaatac ctataaaatt gaagaagtgg ctaaaaacgc
    20461 gtctgataag ctcaaaaaaa attcttctga ggcacaggcg aagatcgaca agttaaatca
    20521 aaccatcaat gagaaaaacc aagaaatcga tcagctgaag caacaagatg gcacaaaagc
    20581 aaaagaggaa gtaactgaag cacaaagaaa gacggcagaa gagttcggtc aattggccat
    20641 tgataaatca ttaagcagaa atgaattaac ggaaaaacta aaagatgtcg caacccaaga
    20701 agtgatcgac aaattaagtc ctgcagacga tgatcaccaa catgacgact acggaagtta
    20761 ctcttttaca ttaggtgacg tgcaaactta cgctcaaaca agcagtgtaa aagaggaaca
    20821 aaattttgtc gtatttgttg attatacaat cggcaatcct caatttaaag atgtcaaacc
    20881 acaaaaaata aaaggtggat taactgtcac tgaaaaacaa gtagatggtc aatggaaagt
    20941 cacagacttt agttatttca ctcgttagga ggaagaaaat gagtaagaaa aaaggagcaa
    21001 gacaacgttt taaaaaacga aaaagttatc ttagatggct ggggatcttt atgattctcg
    21061 gctttttgat tcttgctagt ccgatttaca cacataaaaa ggtagacata ccagatgaag
    21121 taagcacgaa cgcattttta aacacgaagg aactgacgat ggtttcaaaa gaaaaaggag
    21181 cgaaccagca attagttttt gaatttgcga tcgacgatga agatcagagc ttattaaaag
    21241 aactagctaa tcttaattat cgtgtagaag taaagacagc taaaggggat tatcaagcaa
    21301 taaaagccaa agtgatcaag gtgtctgatg attattttgt agtgaagtta gcacatgtac
    21361 cagaagaata tatcgccatg cgtttaacca ttattcctga aaaaattgat ccaaaagtgg
    21421 atatgcaaga accacaagat ctgatttatt acgttcacga agataaggtt aaagatcaag
    21481 tgaaagatga agattatgaa caacatgcga tcaattacaa agtgaaagga tacaaaaaag
    21541 aacaaaaagc agcacaaaaa caaatggaat ccttccaagc aaccattgat ttgaatgaag
    21601 aattagtgaa gaagctaaaa gatcaattgc cttatcaagt cgcagatgat caggaaaata
    21661 ctgaaaacaa gattaatagc taccagcaag aaattgaaac atcgaaaacg caaatgaaag
    21721 atcaagaaga gatcattcaa aaacttcaag aaaaaattga tcgtatgaca aaagaaaaga
    21781 tttagaagga caggtggagt gaaaaaatga agcgaacaaa cttggctgac aaagatactt
    21841 tcgctgagca gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat
    21901 gtcgagaagt gaatttatct aatacaacaa tatctgagtt gaagaatggt cgaaagaatt
    21961 tagatacttt aaaattcaga tcatttgaag ctctatatgt agctgctaaa aaatatttta
    22021 cagctgataa caaataaaaa acaggagtat tttatgtgaa aaaagtatat aaaaaatgct
    22081 tatgaatcag ctatatcacc ttaaccattt gttaatgaga tgggagaaaa cattaaccac
    22141 gattctaacg caaacatcaa gcacataaaa catttaacac tcctcctttc tggctgaaga
    22201 tttcaaccaa acaggaagac agcgtatcaa ttatagcata cactttttct taaattcatt
    22261 tttcatatga caaaatattt ccttctaatc gacaaagtta gaaggatttt ttattgtcta
    22321 aatacacaaa taaccttaat ttataaagag cataaggaaa cgtgggaaca ctttttaaaa
    22381 aaagtgttcc aataggctgt gcctattacg tttctcaaaa ataattgtgg gaacaaaata
    22441 tttaaaaata ggatagtgaa aggatagcaa aggatagtaa aaggatagaa gcaggatagc
    22501 gaaaaggata ggctcctttt cgctatcctt tttaaattgg aggaattaat atggaagtaa
    22561 gaataagaaa tgtagatgag aaaatcgtaa gagaaattga tcgaagatgt gacgaactaa
    22621 gtaaccgaac gggtaataaa tggagtcgta acgattattt aaagctactc gttgaaaatg
    22681 attttgatcg tcctttgatg gaatataaac aagataaatt tgatcaatta ttaaatcagt
    22741 taatagataa attttctttt gttcaaagtc ataatacaaa agtattagaa gaatatattt
    22801 gtcagaataa tcaaattatc gaacttttaa ttgaacaaag tagaggagga gaagtttaac
    22861 atggccaaag ttatagacaa gcatatttat ttttatgaaa cacatataga tgttctagat
    22921 gaaattattg caactcatgc agaggttaaa aactatagtg aagccgttcg gtttctatgt
    22981 atgaattatg attcagaagt ggagaaaaaa gtaaattcca atcaagcaaa gttgaatgca
    23041 atgggaaaag aaatttctat tttaaccgaa atggtttcag agtttgcgga tctccatttg
    23101 aaagatacag ggattttaat tggtacaaat tcaactgttt accaagaagc aaaagagcgt
    23161 gtggaaggca agataaaagc aaatcaaacg aagaaatatt ctcctaagat aaagaaaaca
    23221 ataagcgaat cagtgactac aaaaagtgaa gtatttgatc caacaaggtt cttaaaacga
    23281 taaaaattag aaggagaatg tgatgagtaa atcacctgca gtcattctca cttgtcaatt
    23341 taccttgccc aataaaaaat ctttttcaac atacattgat tatatgacaa gagaaaaagc
    23401 gctggaagaa aaggaacgac gtacgcccga ggaagaacag gaactgcgtc taatcaagaa
    23461 tgctttagag gatttttata ttcctgaagg agaaactttt tcggagagta agacgggtaa
    23521 agaattcagc gaaacgtcga atgaagcgca atcgttgatg aaacatggaa tcaattttga
    23581 caagttagaa gaacaagatt ttaccaaata tctttcttat atgacacgtc actatgcgtt
    23641 agaacaaaag aaagtactga cgcatagtga acaaaaagaa taccaacgct tgaaccaagc
    23701 aataaaaaaa tatcaaacag agccacacaa aaaagcttca ggaaatcttc ctggagtttt
    23761 ttctatggct tcagacgaag tgaaaggaag tgatctgaag gagatcaagc aaaccttcca
    23821 aagaggacaa caaaaaggct caatcattta tcaagatgtt gtctctcatg atcattcgta
    23881 tcttgaaaaa ataggattgt acgatcccaa aacagatact ttagatgaag aaggtttaaa
    23941 agcagctgga agaaaaatga tggagacatt gttcgagaaa gagcaactga atgaaacagg
    24001 ctactggatg gcaacgattc accgaaatac caaacatatc catattcatt ttgcagttgt
    24061 tgaaaaaaaa aatacgcgta aaccatacac tgagattgaa aatggtgtga gttatatcgt
    24121 accgaaaggg aagagaaggc aagcaacatt agacgaaatg aagactaatt atgtacatga
    24181 gttagaacgt ttttcttatg aaaaaaatcg aatgggtatg gagaaacgaa atttattgac
    24241 acggaaaagt gatttgcgta acaccttaac caaagtagtg aaagagccag tacgttatga
    24301 tcgacacgca atgagcttat taactgaagt atataaaaac ttacctaaaa agcgttcgga
    24361 atggcattac ggggatgaaa atcgaaccaa actttcacca tacacaagag agaaactcga
    24421 tgagttaacc caatatgtgt taacaaaaga acctggatat caagaatata tacagatttc
    24481 aaaagcattg aaagaagaag caaaaagtct ttatggggaa agcaagagag aatcaaagga
    24541 tgcagaaaaa aatgctctct ttgatttaaa aaagcgcaca ggaaatgcca ttctaacaga
    24601 actgaagaaa caggatagtc aaataaaacc tttaatagaa aggatggatc agatgcaatc
    24661 tatttacaat gaacgatcct ttcgaacact taaacagttg ccaacgaatg aatacaaata
    24721 tagttacgaa gaattcatga agcgcaggaa acaattccaa caaagagttg tcacgcaacg
    24781 gagtttgcgt caattaaatc gaacgattaa tgcccatgaa gaaaaatacc acgcaagtaa
    24841 agcatacgaa gagcaacaac gaagaattca aatggaacaa gaaagacagt aggaggaata
    24901 gcaatgaaac aatatttaga tcaatggaaa gttatagaag gatcattaag agaagaacga
    24961 atcgaacaac ttcccgattg tttagaaaaa gagcatttgt ttcagataag agaaatgttg
    25021 ataaacgaac agtttgatcc aaatcaattt ttagtcgttg agtacccagc tactggtgtg
    25081 tactgttgta atcatgtgaa gggtgaaaaa tattttatca ttcaagaata tgagggcaag
    25141 ttagcgcctt attatactac ttgggaaatg aatgaagagg ggatcaataa cttcccgtgt
    25201 aaaagcattg aagagtctat ttcactcaca gaatgttagt tgttaagtga ataaaagaag
    25261 taaaagagtc ctgaacgtga ttgtccagga ctcttttttt tcactgaatt gttttccgcc
    25321 aattcattga gtggtgcgat ttccgtctac attatatcaa aagaaaggag gaagaagaaa
    25381 tggcttattc aacgattgga gttgttgttg aaaaaagcag agacaatctt gtttttgtga
    25441 cagaaattca aacaggcaga gcttttgtag tcacagacaa agcggcaaaa gcatatcaaa
    25501 gtggcgatat tttaacgtta aatctgacta cgaagacgtt cgtcgatgcg gcagaagatt
    25561 atccatt
//
LOCUS       DAZUMV010000027        24921 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000027 DAZUMV010000000
VERSION     DAZUMV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.027"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..728)
                     /locus_tag="ACG8FK_001011"
     CDS             complement(<1..728)
                     /locus_tag="ACG8FK_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5096637.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKT"
     gene            complement(778..2274)
                     /locus_tag="ACG8FK_001012"
     CDS             complement(778..2274)
                     /locus_tag="ACG8FK_001012"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5096638.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(2468..4090)
                     /locus_tag="ACG8FK_001013"
     CDS             complement(2468..4090)
                     /locus_tag="ACG8FK_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5096639.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(4418..5731)
                     /locus_tag="ACG8FK_001014"
     CDS             complement(4418..5731)
                     /locus_tag="ACG8FK_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5096640.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(5976..6200)
                     /locus_tag="ACG8FK_001015"
     CDS             complement(5976..6200)
                     /locus_tag="ACG8FK_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096641.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(6220..6642)
                     /locus_tag="ACG8FK_001016"
     CDS             complement(6220..6642)
                     /locus_tag="ACG8FK_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5096642.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(6784..7494)
                     /locus_tag="ACG8FK_001017"
     CDS             complement(6784..7494)
                     /locus_tag="ACG8FK_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS5096643.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(7512..8009)
                     /locus_tag="ACG8FK_001018"
     CDS             complement(7512..8009)
                     /locus_tag="ACG8FK_001018"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5096644.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(8046..8498)
                     /locus_tag="ACG8FK_001019"
     CDS             complement(8046..8498)
                     /locus_tag="ACG8FK_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5096645.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(8633..9238)
                     /locus_tag="ACG8FK_001020"
     CDS             complement(8633..9238)
                     /locus_tag="ACG8FK_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5096646.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(9688..10308)
                     /locus_tag="ACG8FK_001021"
     CDS             complement(9688..10308)
                     /locus_tag="ACG8FK_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5096647.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(10310..11179)
                     /locus_tag="ACG8FK_001022"
     CDS             complement(10310..11179)
                     /locus_tag="ACG8FK_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5096648.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(11202..11609)
                     /locus_tag="ACG8FK_001023"
     CDS             complement(11202..11609)
                     /locus_tag="ACG8FK_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5096649.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(11628..12917)
                     /locus_tag="ACG8FK_001024"
     CDS             complement(11628..12917)
                     /locus_tag="ACG8FK_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5096650.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(12944..13246)
                     /locus_tag="ACG8FK_001025"
     CDS             complement(12944..13246)
                     /locus_tag="ACG8FK_001025"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5096651.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(13271..15364)
                     /locus_tag="ACG8FK_001026"
     CDS             complement(13271..15364)
                     /locus_tag="ACG8FK_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5096652.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(15853..16617)
                     /locus_tag="ACG8FK_001027"
     CDS             complement(15853..16617)
                     /locus_tag="ACG8FK_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS5096653.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(16728..17597)
                     /gene="mdcB"
                     /locus_tag="ACG8FK_001028"
     CDS             complement(16728..17597)
                     /gene="mdcB"
                     /locus_tag="ACG8FK_001028"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS5096654.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(17622..18698)
                     /locus_tag="ACG8FK_001029"
     CDS             complement(17622..18698)
                     /locus_tag="ACG8FK_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5096655.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(18722..19315)
                     /locus_tag="ACG8FK_001030"
     CDS             complement(18722..19315)
                     /locus_tag="ACG8FK_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS5096656.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(19319..20995)
                     /gene="mdcD"
                     /locus_tag="ACG8FK_001031"
     CDS             complement(19319..20995)
                     /gene="mdcD"
                     /locus_tag="ACG8FK_001031"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS5096657.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(20985..21284)
                     /locus_tag="ACG8FK_001032"
     CDS             complement(20985..21284)
                     /locus_tag="ACG8FK_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS5096658.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(21386..23032)
                     /gene="mdcA"
                     /locus_tag="ACG8FK_001033"
     CDS             complement(21386..23032)
                     /gene="mdcA"
                     /locus_tag="ACG8FK_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS5096659.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(23039..23950)
                     /locus_tag="ACG8FK_001034"
     CDS             complement(23039..23950)
                     /locus_tag="ACG8FK_001034"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5096660.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            24070..>24921
                     /locus_tag="ACG8FK_001035"
     CDS             24070..>24921
                     /locus_tag="ACG8FK_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5096661.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLG"
BASE COUNT         7080 a         5321 c         4235 g         8285 t
ORIGIN      
        1 tttgtttttt ctaaaagttc ttctcttcga agaatggtca aaacgagata ccgagaaaat
       61 cgtatgaagt cctctcctga aacaatataa ttatatgata taagaacctc ttttaatgtc
      121 ttttggacag tcggcatgat attggaaaaa attgtactga tttgttgagg aaaccttgcg
      181 tgttggatca ccgtttctgt tccaacaaga gataagaaaa ttctttttga attttcttca
      241 gcaatgattt ttagtccttt tgtcgacgaa acttccagtt ctaatttagg attgcgctcg
      301 atccaacgca aaatcgtttt tatttgtaat gatacggctg tttttgaaag aaagaaaatg
      361 tctgctatct tttgcatact gacataccct ttttgaaaca ataagtacgt cacaatagtt
      421 gaagctcgga aatccatgct ggaatagatt ttatgtttcg tcacctcttg tatgatttgc
      481 tgtttagttt cagattcaat tgattctaaa aaataacctg aacgtttatt tgactggata
      541 atgattttct tctcgtagat agcaagcata tttttgattt cagtttggat cgttttcttg
      601 ttggtaccta aagcttcagc taattcttcg cttgttatcc aatgattctt atttcttaat
      661 actacaagaa tttggagttg tcgtttattt aagtccattt gcttacacct cctctttgtt
      721 ataaacatac aggaaaaacc tctcaaatga caatagtcac ttgcagaggt ttaaaaatca
      781 tttgaagaaa gatctatatt caaggttttt accattagac tggataattt gtttataata
      841 ttcaaaacta tcttttttca atctcctaaa gcttccgctc ccatcatttt gtctatccac
      901 ataaataaag ccatatcgct tcctcatttc ccctgttcct gccgatacga tatcgattgg
      961 tccccaccat aagtaaccaa tgatttggca accatctttt attgcctcag caatttcctg
     1021 aagatgtatt tttagatact cacaacgttt tggatcgtgg attcgattca attcatctgg
     1081 tgcttcatct agtccaatgc cattttcaac tatgaataat ggtaattcat aacggtctgt
     1141 caattcattc atgacataac gaaaccctag aggatcaatt ggccaactcc aaggttcggg
     1201 agatttttcc ttcaaaaagg gattatcgaa accatttgct ccgccagtat ctacgcgcat
     1261 ttctgcttct tttttataga cagcgctacg ataataacta aatgcaatat aatctgccgt
     1321 gttagcttgc aagcactgtt cgtctccatc taacattact ggtgcacagt cttcttcctt
     1381 ccatgtcctg taaatataag ctggataatg ccctcggcac atgacatcgg agaaaaacca
     1441 tgaattgcgt ttaaattgca gtgaaccaaa tacattctct ggatcacagt catatgcata
     1501 agtcgctaat gcagaaaagc tacacataat acccatctta gcatttggat cgattttctt
     1561 taataattga gtagcacggg cgttcgctag aaacatatga tgacaggctt gataaatcac
     1621 tttttttgtt agtgtagcaa tgggttcaag tttatctcta gcagggaatg taaccaatcc
     1681 agcagctgca taaggcatac gaaaagcatt attgatctca ttaaaggtca tccaatattt
     1741 cactttccct tggtagcgtt caaagacggt ctgcgcataa aattcaaaaa agtcgattaa
     1801 ttttcgattt tgccaccccc catactctcc taccaatgct aagggggttt cataatgact
     1861 aagagtaatt actggctcga tatcatatcg atttaatgta ttgattagtc tgtcataaaa
     1921 tacgagacca gcctcatttg gcttctgttc gtctcctctt ggaaatattc gtgcccatga
     1981 gatgcttgtt cgaaaagctt ttaaacctaa ctccttcagt aaacgaatat cctcttcaaa
     2041 tcgatgataa aaatcaatcg cttcatgaga aagataatgg agtgattcgt ctaaagcaat
     2101 ctcccaaatc tttttttctt cattccactg aatactagga tgctggttca tgatcccgat
     2161 aagcgtatct gtcacattag gtaatttgcc gtcttccaac catgcacctt cacattgatt
     2221 agcagctact gctccgcccc ataaaaaatt ttctggaaat atttcctgat ccataactat
     2281 ctctcctcgc tatcaacaat ttcttacttg aagtatagct cgattcatca aaacaagata
     2341 ttctaactat ttcggcagaa gagaagaaaa aaattgaatg ctactcttca tttttaatat
     2401 ttatatataa caaatacata agacgatccc cgaaagaaaa cactcgttaa ccatctaagc
     2461 aaacgattta ttttacttcc aacacaaatg cttcataagg tcgtaaatca actttgccgt
     2521 ctggtaactg tttcagagga taatttgaca caataaccct taaagcttca cgtggaatag
     2581 tgaacgtctg cttttcactg gacatattac aaataacgag ccattgctgg tcttcatata
     2641 tgcgagaata ggcaaagaca tgctcaggcg tcttttccac taattcatag tctccccaga
     2701 cgatgatgtc atgttttcta cgcaaatcga tcaatttctt gtacatataa aaaacagaat
     2761 cttgatctgc taacgcttct tctgcattga tttctttata atttggattg actcgaagcc
     2821 atggaattcc agtagtaaac ccagcatgtt cacgggcgtt ccattgcata ggcgtcctag
     2881 cattgtctct tcctttcgcc ttgattgatt ccaagatttc ctcttttgta aatccagaag
     2941 aaatcctttc gttatacata ttgattgtct caatatcttt tgcttccgcg atatcatcga
     3001 ttgggcaatt cgtcatgcct aactcttctc cttgatagat ataaggggtg cctttcatca
     3061 tatggagcaa gatagcaaac atcttggcac tttcttttcg atacacatcg tcatttcccc
     3121 aacgagaaac aattcgcgga agatcgtgat tattccaaaa taaactattc catccttcat
     3181 cacctaaaga tgtttgccat ttggataata cttttttcag ttcagagata ctcaagggtt
     3241 tcaaatccca tttgtctttg ccttcttgct gatctaatcc tatgtgttcg aattggaaga
     3301 ccatggataa ttcatttctt ttaggattgg aataaagttt agcgatttcg gtagttgctc
     3361 cccatgtttc gccaacagtc atcagatttt tgtcaccaaa agttgcttga ttcatttcct
     3421 gaagatactc atgcagttta ggtccattac ttgtgatttc tttatccggt tctttgccaa
     3481 tcatatcgat cacgtccata cgaaagcccc caataccttt atcgatccag aaattcatca
     3541 tttgataaat ctcttggcga acctgtttat ttttccaatt caaatcaggt tgacgcttgc
     3601 tatacaagtg cagaaaatat tgccccgttt tttcatccaa ctgccaagca gaaccactga
     3661 atattgagcg caatctatta ggctcttttc cattgaccgg atcacgccaa atataatagt
     3721 cccgataagg attttcttta tttttacggg cctcaatgaa ccacggatgt tcatcagaag
     3781 tatgattcac caccagatcc atgatgatac ggatatttct tttctttgct tcttcaatca
     3841 gttcttccat ttcttccatc gtaccgaatt ctgccatgat tgcttgataa ttactgatat
     3901 catacccatt gtcatcattc ggagactcgt atactggaga aagccaaatt gcatcaatac
     3961 ctaaatacgc cagataatcc aacctggaaa taatcccccg caaatctcca ataccatctt
     4021 gatttgaatc ttgaaaactt cttggataga tttgataaac aactgaatgg tgccaccatt
     4081 tttgttccat gattttcctc ctataaatgg atgtttctat tttttgctta atcatttatt
     4141 ttagtctctc tcactaaaaa ttgtaacgct ttcaaatagc tattgcacct tgttatcggt
     4201 aacagaataa aacaaaaaaa cagctgagga aattccctca actgtttttt gaaaggattg
     4261 atttatgcag ggaagaatgc tgatttcaca acttctttat aaacggtgtt cactcagctg
     4321 tcccttggta ttagctcaaa aaacggcaaa atataagaaa ctattttttc gtttttttct
     4381 ctaatactca ggccagaacg ctgatttcac aacctcttta tttcacattc aatatccaca
     4441 tgaatcccac aaatacaaag aacagaccat acatgattgg agatacttct tttcctcttt
     4501 tagctgctaa catggtaatt ggatagaaga taaaacctaa tgcaatccca tctgaaatac
     4561 tataagtcaa aggcatgccg atcaagatca agaatgaagg gatcgcaatc tctaagtttc
     4621 cccattcgat ttgtcgcaaa gattgtgcca tcaatactcc taccataatc aatgctggtg
     4681 cagttacttg tgaagtaaca actgatagta atggagaaaa gaacaagcct aacaagaaga
     4741 aaatccctgt agtaatagct gtgatacctg aacgtcctcc aacggcgata ccagctgatg
     4801 attctacaaa tgcaccaaca ggagaagtac caaataagga acccgcaagc atagctgttg
     4861 aatcagaagc taaagctttc cctacacgag gcatcttgtt gtctttcatg aaacctgctt
     4921 ggtttgctaa tccaactaaa gttcctgctg tatcaaagaa agtcacaagc aagaatgtca
     4981 gcactacgac ccacatttga agagaattga tgtcccctac atgttttaat gcaactaaaa
     5041 atgttggttt caaacttggt gctgcggcaa caatttcaga aggcattgga atcaaacctg
     5101 tgaccagacc taaaatcgtt gtagctgcca tcccgatgaa gatgccacct ggtacacgcc
     5161 gaacaagcaa gatagcagtt acgactaatc caaaaatcgt caaccaagta gaaccaacgt
     5221 tgagtgatcc taatcctact aatgtagatt cattcgaaac gatgatccca ccttcgctca
     5281 gaccaaggaa agcgataaat agaccgatac cgccagaaat cgcatatttc aaatctgctg
     5341 ggatcgcatc aatgatcatt tcccgcagct tgaagattgt gatcaaaata aaaatcaaag
     5401 aagcaacgaa tacgccggct aatgctgttt cccatggaat ccccattcct aaacaaacag
     5461 aataagcaaa aaaagcattg atcccaagag caggtgccgt tccaattgga taacgggcta
     5521 atacccccat taaaacacag ccgatcgcac tggctaatgc agtcgcagta aacactgctc
     5581 cttcatccat tccagaagca cctaatacag ttggatttac aaataagata taagccattg
     5641 agataaatgt tgtaaatcct gccagtacct cacgttttac cgtggtgttc aatttttcta
     5701 tttcgaagta ggaaagtatt ttttccttca tcttccatcc ccctaaatcc gaacattata
     5761 aataaagtcc gttaaaattt cgatttatta ataaaacaac gtgttttatc atatactaaa
     5821 acaaaacata agtaaacaaa aaattcggat tttaagcaaa atccgaacgt tccatctgat
     5881 taaatccctt tatctcaaag ttcatgaaca ttcactttgg attacaagaa tttctttatt
     5941 gtgttaataa agtttcttca ttctagtctc aaaaatcaat agtgtgattt gattttacca
     6001 tttcgtttct gttcttcttt gatgaattct tttgtatcga caggattcag attatttagt
     6061 ccgccaaaac ctgtatcgac catttttccc cagccttttg tcttcttttt tcctgatttg
     6121 agattctttg ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa ataaccttct
     6181 aatcttccca ttagatccac tgtttttcct cctttttatt caatcagacg actcagcttt
     6241 tcctataaca aaataattgc cttcagggtc agaaaaattg aaagtcaaga tgccattgtt
     6301 atctgtgatt tctcctggtt cttccatgtt ctcgtagata gtttcaaatt catccgtaaa
     6361 aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa aacttctgca caaatacttt
     6421 tgggaatacg cctaattcta attcttttga gatgctgagt acaatactct taaattcttc
     6481 tggcaattcg attgtttcag aaatttctgc accaattttt tctacaaaga atcggcaaat
     6541 catctctacg tcattgacat aaagcatcac acgtgttttt tgtatcatca tttgaccctt
     6601 ctttctattt gattttcttt cctgtctata aactgattcc attatatcac gaaagcgcaa
     6661 acaccttgtg tttgatacta ttcaagaaaa ataaagcaat agtttctagg attgctttcc
     6721 tccacaaact ttttttacaa aaaaaaaacg gcaatgaatg ccgctcttct tatttcgatt
     6781 cttttataat tctgcttctg attcatcttt ggttagtgtg tgtctaacac tagttacgcc
     6841 aaatgtttga tcgatttttt ccttcaacgc ttcgattgcc tgtgcgccac gtgccgcttg
     6901 ttcagcattg atggattcaa tgaccagcac cgctgcttgt gtttcttccg tcagcatcgt
     6961 tctttgggct tcacgccagt tgagacagcg acagatcgct ccttgttcat catagtagat
     7021 gatttcttcc ggaagtgccg gagcatcgga atctgcacct aaaggcagga atgattcgcc
     7081 accttgggct tttcctaaat gcagatttcc tgcgatcttt tcgatatctt ctccgccgca
     7141 aggtacgcca aaagacaagg aaatactatt atagatatct acaagcggat tgattgggaa
     7201 aaattcccgt tcttgatttg cacgtttcag taaagcttca atagaagaac gtgcaccttt
     7261 tttagtcttg aatgtggtaa atgcctgacg ccactgagca atgacttcgt tttggctgaa
     7321 tgtttcttct gtaaggtaat ttcttgcttc ttttgctgcc tcattcaata attgatgata
     7381 cgtttcttca ctttcttttg ttacgtggtt atcgatacca tttacggata agatttcgat
     7441 ggttgcttca ggaaatactt cccagaaatt ggtatccact attacttttt tcatctgttc
     7501 acacctcttg attagaataa ttgcttgatg aatagatagt ctgtttgggg atcatctccc
     7561 atataaaaaa catgttcgct gtatttgata aatccttttt tacgataaaa agcgagcgca
     7621 acatcgttgt gttcccaaac accgagccaa atggctgttt tttgcttttc ttttgctttt
     7681 tcaatagctg tatctagtaa aaatgtaccg ataccttgat gtttaaattt ttttcgtaca
     7741 tagattcgct cgatttctaa aaaatgatca gccatttcct cagattgact cgttcctgta
     7801 tttagtttca ggtaacccgc aggctcttga tcgatactca ctaggaaaaa atcggtttct
     7861 ttatctttta gctcttctgt aagtttttct agtgcataag cttgtgtcaa gtaatcagct
     7921 aaatgctccg gtgaattatc ttttccaaat gtatctgtaa atgtttcgat actaatttgt
     7981 tgtagtaagt ctacatctga aggcgtcatt tttcgaattt cgtatttatt cacggcttct
     8041 tcctttcaat agatcctttt attgcctttt ttgacaaaat tccaatcttc gctaacattc
     8101 ttgcatactt tatttaaaag atattccaat tgttttgctt cttcgtcaga aagaccattt
     8161 aatgcgactg cattggagta gttgttttct cgaatgatga aaggatagat ttctgctcct
     8221 tttttagtcg gatagagttt cttgattttt ttattttctt tatcttccaa acgttcgatc
     8281 atcccattgg attctaattt cttgattgct cgggcagtcg ttgtacgatc tactttgatc
     8341 atttctgcta gtttttcttg gatgattcca ggattttcgc aaatacgtac taaataaaga
     8401 tactgtcctc ttgttaaatc gacttctttg aattcgatat tgctaataga atctagcgct
     8461 cgggcaatca ttccgatgtc tcgaaggatt tcagccattt catcccacct ttattttgtt
     8521 tgtaagacta gttcacctct ttttgttgca aatgcaacta ttttcaataa aaaaatcaag
     8581 ttctttttta ataactagta aagtatattc ttttcttctt ttacgaaaaa gctcaatcac
     8641 aatagatctg gtttttgttt tctaaaataa gtaactgttt cttcgttgtt cgcttttctg
     8701 atagctttgg caactcttct atactaaaaa atcccatgtt ggaagtttca ttattctcaa
     8761 taaacttggc ctcttcaccg tggatcgcac aagcaaaaac aagtttataa tactgaaaaa
     8821 gctgtggaat atctttttgt ttattcgtat caaaaactgc tcgcaactga gtagtttcta
     8881 ctgttaatcc tgtttcttca tagacttctt tacgcacatt ttcttcagga gacaatccta
     8941 tttcagcaaa tccaccagga agtgaccatt ctttcgtccg tttatcttcg attagtagga
     9001 ccttgccatc ttttttgata taggcacgaa catctacttt tggtgttgga tgtccttctc
     9061 caatcgttgt gagcgctttt atttcttcga aagtttcttt tcctatttca ctgatcagtt
     9121 ccaatgaaat cgtccggatc tcctcaaaac gctctttatc aaatacatct tttccataat
     9181 atagaccagc ttgagaaagt gcgagtagtc ttttgtattg agccaacttc ttgttcattc
     9241 tcttcccttc ttttttagca aatgtatgac tatgtaatca agtatagaag actggtttaa
     9301 ttacggcaaa taaatataat gaaatctgca ataaataaaa gaatgttaaa aaactcgaga
     9361 acaaaagaca ttcgacttct ctggggctgt gaataaatgg cgttcaaaag cttgcatctg
     9421 cggtaataag ccccaccaaa cgaaaaatat gacgagctat tcttgtctgg ctgttcttat
     9481 taattgaggc aggacagctt tttcacaacc tccccactaa acggtgttct cctaccaact
     9541 cctcggtcac aagtcacaat attcatcaaa catgagaagc tgtttttttg aaattgctcc
     9601 ttgtgactcg gagctgaacg acaggctcac gacctctcgg taaacagcgt tcaaaagcca
     9661 atttttcgat attaagccat ttctttgtta cttattgtca ttgatttctt tttctaattt
     9721 atccaatagt ggagtgtaat ccacttgagt atagtctgtt acatcaaatt catatagtgt
     9781 tcctaactgg aatttgtcat attttcggtc ggcgatgatc tttttgaatg cttctttcgt
     9841 aatatgattg gtccctttcg ttcgatcttc caatacgtca ccatagtgat aatgatccat
     9901 gatatagctc aaatgacgat ctgattcacg atcacgtttg taccgacgtt gccataacac
     9961 ttcaaagtct gctgttaggc gaatcgtgat cacctgatat ccgtatctat ctgctaattc
    10021 ttggagtcgt gctttttgct tatcactaaa aggatactcg gacacaatca ctttctttcc
    10081 tgcttccata tacaaatcaa gtgctttgta atagaaagga taaactttcg tcctttcttg
    10141 ttccgcccgt tcttccaaat tgttaaaccc gatggattct gcatagtatt ccttgatttc
    10201 atcgagcgtg atggcataca tatctggaaa tcgttcccgt agtttattca taagatatgt
    10261 ttttcctgta ccaggactac cagccagaag aatgaaatac tttttcatct catttatttc
    10321 ctaagttaat tggactttcc aagttcataa acagtccaat cttatgcttg acaatttctt
    10381 ttactgcttc atttgctgga atcacattat ggcgaagaga cttgttgatt tctgtttcac
    10441 taaaggtttc tttagctact ttcgtccagt tgactaaaag ctccgttcca acattgaatt
    10501 tgcggatccc gttattgatc aattcaggat aatcttcttc tttcacacct gttcctccat
    10561 ggataactag ggggacatca actactgcat cgatttcttt cagcaaggga atgtttactt
    10621 ctgtttttga tttgaattgt ccatgattcg taccgatagc aatagctaat gcatccacgc
    10681 ctgtagcttc tacaaatttc actgcgtctt ccggtcttgt atataccttg tcattttcag
    10741 cgacatggat tccttcttct gtaccgccga ttgttccaag ttctccttct acggaaacac
    10801 cgtgcgcatg ggcatattct actactgctt ttgtcttcag gatattttct ttgaaaggca
    10861 gatgggaccc atcatacatg acagaagtat atccgctgtc gattgcttct ttgatatcat
    10921 caaagtctcg ggcgtggtct aagtgcaaga ctgcatcaac catttcttct tctgcaatag
    10981 ctgtcattac attgactaac acatgcatgc cgatatattt agcggtatcg acacttgttt
    11041 gaataatgat cggcgaaccc atttcttttg ctgcgcctag catagcagga agcatttcta
    11101 ggttatgcgt attaaaagct ccaactgtaa aattcaactc ttctgctgtt tttgttactt
    11161 ctttcaatgt tgtatacatc ttttattgcc accttatctt tttatttgtt tcctttgatc
    11221 gttacttgtt ttgcaatttg tcgcatctca tccattttgc ccatgtagta atcgatccct
    11281 tgatcatcac cagcacgagc agcatctgca cgcttctggt tatagaggct catcagtttt
    11341 ttatacccat ctccgatcgt ctgtttattt tccaaactct tttctaaata ttggatggct
    11401 tgatcgttca catacaattc agaaaattta agtccaactt gttcataaag tttcgctaat
    11461 ttatgcttat cttgggtagt ggaaatctct tgtttcaatt gtgtaatctc tgattgaatc
    11521 ttttcggtcg cttctggcga tagggctttt tcttcttttg ccagaacttt tttcggttct
    11581 ttttcttttt tcttaaaaaa tccaaacatc attttcccta ctttcaatta aaatgctttc
    11641 aaaactgggc ttaagatcca ggcatataag agtgctgcgg ctactgtatc tacatcggtt
    11701 gctgtagcat tgttgaatcc catcgaatta aagatcgtta ctagcaaagc gggcaataat
    11761 gtaataaaga aaccatgagc aataccgcca atgatcgctc ctcggcgtcc gccaactgcg
    11821 ttgccaaata tccctgctgt ccctccggca aagaaattgg ttagcatacc aggtaaaatc
    11881 atcgctaagc cgaacatggg caaagtaaac atggcaataa tcgtaccaat cgttgtcgta
    11941 atgaaaccta aaatgacagc atttgggcta taaggaaaga aaactggaca atctaaggca
    12001 gggatcgcat caggaactaa tttcatagct atccctcgaa atgccggcac gatttcacct
    12061 aacagtagac gtacaccagc tagcaataca tacacaccca ctacgaattg gatggcttgt
    12121 aaaaaggcaa acatgatata gttttgatct cctgataatt cagaagcgaa tggttcgcct
    12181 gcaaataaaa cagtgatgat atatagcggg accatcacga ccatgacaga caaataagta
    12241 tcttgcagaa attcgaaatg tgtcggcaac ttgatatctt cgatcgactt tttattttcg
    12301 cctttctcac caaaaagctt agcaacaccg gcttcaaata agtacccgat cgtacaaaaa
    12361 tgacctaaag caatatcatt tgatccagtg attttacgaa tgatcggttg cgctactgcc
    12421 ggcatagcaa tggcgaaaca agctccgata aaaccaccga ccaagatcaa tacgatccct
    12481 cgcagaccgg caaaatagcc aaagactgtc gtcatcgttg ccatccataa aattgcttga
    12541 ccagttaaaa aaatgtactt ccattttgtg aaacgagcca aaataatatt gaatacaaaa
    12601 atggctaaga acgtcagtgc aatatctcta cctaaaccca aatcattcat tgcttgacca
    12661 ttgatagatt caatagaagg gatgatccct tgcatatgaa atccttcagt aaagattttc
    12721 ccgaaataag ttaaacttcc aacgatgatg ctcgaaccag cactcaacac ttgaaatcct
    12781 aggagtgtct ttaacgtgcc agagatgact tgtcctgctg atttcttctg cagtagtaga
    12841 cccaacatag caatcaatgc aatggtgatc gatgcctgag tcaaaatatt ttcgatgata
    12901 aaattgataa cgcccatgtt tttaccctct tttctttttg tatttagata actcccttat
    12961 cttgcagaac tggggtgatt ttttcttcaa tttcattttt tgaaacaata tttttcaaat
    13021 agatcatcgc cgttttagga tcaactgcga acttttcgaa ctgcgaacga aaattttctg
    13081 ctgtgatgat cacatctggt ttcatggaaa ctgcggaaga aatatcacaa tggtccagtt
    13141 tggcttctac tcctaatttg ttcaaaacat cctcagcaga catctgtgct gcaaaactac
    13201 ttcctaaacc tgcaccgcat acgaataaaa tgttcaattt gttcatcttt gttctccccc
    13261 gtatcattta ttatttctca actactgaaa aaagcagttg tttgaaatct tcgatatttg
    13321 tgcattcaat caatcgatcc aacttctctt cgttgttgat caattcaatt agttctttca
    13381 tgatgttcag atgcgagtag gagtctactg ctgccaaaca aaaaataatc tttactggat
    13441 ccatttcagg attaccgaaa tcgattggtt gctcaatcgt agctacgctg actcctaatt
    13501 tattgacccc atcttctggt cgggcatggg ctagtgccat gtgtttgcca atcacgatat
    13561 acggtccgta atcattcact gcacgaatca tcgcttcgac atagcgctct tcgacaatgt
    13621 tttctttaac caatggtcta gcagctagtt caatcgattc ctcccaagta gtggcttttt
    13681 ctttgatcat gatattgctg tccgtcaaaa tatcttttaa cataggctgg atctcccttt
    13741 tgttgatttc taaattgttt gtttcgaata atttttctaa cttgccatag atgattcgat
    13801 ctactcggcc accactttct ttcatgacat ctattatttg agtgaataac tccgtcgatt
    13861 catgactgtt aggctctaac cgctgtagtt cttcatttct tttcaaaaag tcttctataa
    13921 ccaagatatt cgtctcagtt aaaatcggat cgacaactaa aagcggctta tttcgataat
    13981 tcaattgaaa ggtgctgaaa accagattga catccaattt atctactaat tcgatttctt
    14041 ttgaacttaa aactgcagta atctcgatct gaggaaactt ttctttcaga ttctctgcta
    14101 acagatttcc agtagccatc ccatggttac agacaacgac tgatttataa acataattca
    14161 agtctcgctt gattacactg gcgatcgtcg aaaagtggat gacaagaaaa gctatctcgt
    14221 cctccagtat cttgccacca attacttctt cgatcgtagg aacaaaatga gtgactgcgc
    14281 ggtagattgg gaaataattt gttcggatgt tttctttcaa tggattcatg acttggatat
    14341 gattcttgat tcttacaatc agtgcagcca tgtgcttata gagattttcg aataaagctt
    14401 cttcctttag atggaaaggt acgtgtgtct cgttctctac aaattggatc aattggatcg
    14461 ttagtaattg agcttgtacc cactcgattg aattggagat atctctagca ttgaacgtct
    14521 caatagtgaa acgaagataa tttctttcta cttgcgggac attgtccaat tgaaattcct
    14581 cgatcatgtg gtcaataaat gcagaaaagc tactgtcttt tatttctgta tttttccaat
    14641 taatggcaga gataaattca tgtttcctga ttcgctggat ccagatcaat gtaaaaagca
    14701 ggatttgttt tttataaata tcatcttctt gtttcaacaa cttattggaa tagatctcct
    14761 gtattttttt gatttccacc aaaggaaaat aatggagaaa gattttttga gccggtgtta
    14821 attgtgcatc ttgattcata aagaagtcgg tcattcccag attctgattg ataaaatcat
    14881 agatcatcgt acggatagat cgttctgaac ctttataaac cagtccttgc tttccataac
    14941 tgacgatctc tatcccataa ctgaccagtg ttgctcgtaa ccgtctcata tcttcatcta
    15001 atgtactttt agagatttga aattcttctt cttttttgtt tagatggatc gggattcggc
    15061 tgtaagcaac ggctaaaact agatcaaaaa tacgttcgcg ccgattcaaa aagccgtctt
    15121 tcacatcttt tatttcagtt tctaatcgag cttgcttttc cttttccagc ttcaacaaaa
    15181 aacctttgtt gcgaattgta cggatctccg gaagcttttt actgctcaac cagatattcg
    15241 cttcaagaac ttcctgacgg atcgtccgct gactgacatt gaactcactt gctagatgtt
    15301 ttgcttttac tggatattgg gctgtcatga ttctgtgaag tatttcaatt gttcttgttt
    15361 tcatagaaat gatctccttc tgatttcaag tatatggttc cgcttacata ataaaaagta
    15421 gatatttctg ccacttgaag tggaaagaaa gtaagcaaag caaacaaaaa ataagccata
    15481 tgctttttca tctcctcaac gaaaaagaca tacgcctatt attacaaaaa ttttgcccat
    15541 ctatacaaaa aagaggttgt gaaaaagctg tcctgcatca agtaataaga acaactaaac
    15601 taaaatagtg cttcctattt ttcgtttggt tgttcttatt atcgcagatg caagctttta
    15661 aacaccattt agtggagagg ttgtgacaga gatgttcagc cttgagcgat aagaagcgat
    15721 tttgaaaaat cgcttcccat attttacaaa atcgtgcctt attgccgata ggctatctct
    15781 ggcacaccgt ttattcagaa aaaggagctg cgacaaggct tttgtcacaa ctccgttcat
    15841 aaatatttcc tgtcaatcca gcataatcgg ctgaataaaa tcggcttgcc atcttttttc
    15901 tttctcagaa aaatcaaaat accatatctg agaaaaatac tgtcgttgtg tcttacttcc
    15961 tgagatcaat cgcccatctt ttcgatcgac tgaataatca cagcatctga aatcaattcg
    16021 gatggcaaaa ctattttcgt ggatctgtcg ataatttcct acttcctgaa tagttatttt
    16081 tttagtatga tttcttacac cgtctgcttt gtttttttgc agaactttct gatgttcggc
    16141 aaacagcttg tctgtataac agcttctggc tttttcagga gtttcttctt cccaagcgga
    16201 ttggatcttc atgaatatct tttggatttc cagaaactgt gctttctttt ctttagccgt
    16261 tcctgacatg cgggacaata tctccgcttt cagccttttc ttttgtcttt tattcttgac
    16321 gatagaaaaa gcagaaaccc ctcctactga caaaacagct aatgcaagaa atcccgtttg
    16381 ctcataacta gaaggacctg tacctggacc ataataccca taagaatctc tgccgtaaga
    16441 acgactgcca aaataactgc ccccatatga acgaccacca aatgaacctc tgcccgaact
    16501 tcgaccgcta gatgtacctc caccatgacc acccgcgaca gcgaatcctt gggtcggcag
    16561 agcaaacaaa cataggccca ctaacaaaat gattgctaaa taattgattt ttttcattgt
    16621 cttcctcgtt atcttttatt acaaaaaact atttttatga tagccttgct aaaaggaaga
    16681 gtcaataacc agatgcataa actgtttttt tagcaatttt gtcttattta gtcattgagt
    16741 aacatcggac aaatgtatgt gtcggcagtc cataattcct ctatcttcag catgaacaag
    16801 cttgcagaca agagatctgc gcttcctcca ggagagattt ggttttcctg acaaaagtgc
    16861 aacagtgctg taaatggacg atttggtaaa gcactttgac tagcagcatg agcaagtttt
    16921 tgcatttcca ctaacgtttt ctgatcactg cgatgcaata cgcatgtgtc atctagcgaa
    16981 ctcatcaaaa ctaacaacat atgcatttgt ttctgtacat atgaatcatt atctagctcc
    17041 ttagctgctt gaattgcaag cctgatatgc ggatacccta atatcgcttc ttcatatgca
    17101 ccatttacgc catatttttg cttcacccta tgaccatgag tggttttttt atgacgatat
    17161 tttgtatcag gaaaagcagc aagcttgctg acatcatcaa gtattttcga taaagaaaaa
    17221 ggctgttcca tactgatttt ttgtgcaatg acactgatca atagccccat tgcccaaatc
    17281 gctcctttat gggtattgat gccatttgta gcaatcagca ttgccttttc cgcttttcgc
    17341 ccaatctcag caattctttc tcttaactcc tgatcaatag catgatgcca tgctatttga
    17401 gccatttctt caaaaaaagg agttagcgtc ttagcagact taagaaataa agcatagtcc
    17461 atatcggaat gtgcacccgc atccagtgca tccactaatc caggttttgg acttaatgtt
    17521 acttctgcga tcaatgcttc ctctaccaac gcagcgagtt gctgagtcac tgcttttttt
    17581 cgttcgtcat ttatcatgac actcactcct tcttcctact attacttgct gaaacctttt
    17641 ttgatagcta cccaaatgat atgaagtaac cagcttaaag caaatgcaca acttgccgtc
    17701 attagataag ctacaagaat catgactcct gaaggtaact gcagccaact atctttaaac
    17761 aataataaca gtacttggaa aacaaataca ttggctaaat acgtacgata agcataaaca
    17821 gacaaccatt tgaacaatgg caattttggc gaatcgttta caatcatttt ggaagcgcct
    17881 atgaagacag caaagatgat gactaaactg tataaacttt ggatcgtatt caagtaaggt
    17941 gcattcgcaa agctcaagtc tcctggatgt gcgagcaatg aacggacaga aaagaacgcg
    18001 ataactaagg caacaggtaa gaaaagataa cgtactctgt ccaaatattg atagactttt
    18061 tcgtgataaa caaatgcggc taccccataa atcatgaaac tagcaacaaa ccgatctaat
    18121 aagtaccagc tgtcatgata aggcccagtc aatacttgtg tttgatagaa cagataccat
    18181 gcgacatata aaatcgttga accaatcaat actggccata catatttttt attagcaaat
    18241 actttgtaac cgagccaaac aaaaaacggc atgaacaatt ggatctgcag catcataacg
    18301 gtataccata aatgaggcgc tccatcacct gtgacatatt tcagtaaaaa gcttgcagta
    18361 tccgtatacg gagtttgttg ttggactgct ggaaatagcc aaagataagc cgtcgtccat
    18421 agaagatacg gaacgactag ttcaaaaaac ttttctttca aaaattccca ataagacaaa
    18481 tgctgacttg tttttaccat attataaaca attgcaaaaa taaacattgg tgctgtgtat
    18541 ttaacgatga cgtaaaaggt accaagaaaa gctgtatttg ttgcgctgtg gctgtttgcc
    18601 aaagcaaagc tcaatatcgt ttgcatcata acagccgtta cagctgatac tttctggaag
    18661 tcacttacgt tgattttgat ttttttgttt tcaaacatct ctttctctct ctttttcttt
    18721 attaccaaag ttctttggtt aatttaggtc cttcgactgt tttcaataga atttttttct
    18781 ctggctctct caaatactct tttagtgaaa aaccgccttg ccgagtcact acttgggtat
    18841 cgaattgctc aaaaaaagca ggatgcgatt gaattacctg aattggcaat tcaatcggtg
    18901 aatttgcgta cagcagcaaa tcaaaatcac tggtttcttt tactgccgga atcccagtcg
    18961 ataattcaaa tcctaagctg ccgccgattc cccatttaca tcctgataat atgtttctag
    19021 cagcatgaaa ttgctgataa acaggataat ccatgatttt ctcttgtcta tacgattctt
    19081 gttcgatgat gtcccacgga tgaatgactt gtttgacggc atgttttgag atttctgttg
    19141 accatctctg attgcgggca ggtccacgta aaccaatcgg tagtatttct gcgtgatccg
    19201 tcgacgcacg acgaactgtt ccgaacatct tttcagttag aacgacccat tcaggcaagg
    19261 tgaacccttc tatttcatgt ttatcaaaaa ggacaatatc atgtgcttga atcatttttt
    19321 atgcatccca ttcttcgtcc attttggcac ggactttatt tgtttcgaca cgtccgaatg
    19381 ttttggctgt ttcgtttgtg tatcgatttg tcagcatcgt gtcatttgta ttgcggacat
    19441 cttgaatggc ttcattgatt gcttcgatga ctttgctgac attttgttct gaagcagctt
    19501 ggtcggtgat atcttctagg aaatgataga tcgctcctag ttttgtataa ttattgatat
    19561 cgtaagcgat agctggaact ttttcagcag ctgcttcgat ttcagcaacc gaacgttttg
    19621 tcacacgtgc agcactttct ttagacatcg cttggacggt aatttcttta ttgtttaaag
    19681 caatcaagcg gcttgattgt aatccatgtg ctaaaaatgc acctgaaata gcatttcctg
    19741 caatcaatcc aataactgga tgtccagctt gacggagttt tgcataagca gcagcactat
    19801 tagccaaagc cacatggata ccgatcaatt cttcgttata gccgtatgct tggcttggta
    19861 catcgatcac aacgatgatt ggccgtttca cttttttgtc tttatcttct tcgtaaacgt
    19921 gatccaaaat ttgtcccatg cggaaacctt caactaaacc aacttcaccg tttcgtacac
    19981 ggtacatcgg gtttttagga tcaggtacga ttgatgtcac gatggcttct tctatgaatt
    20041 tacttgattt accataatac acagtactaa tagaagaagt tggatttttc atgccagtta
    20101 gtgccgcaaa ccaatcgtaa ccaacacttg ctgcagctgg ttcatttcct tctgctactt
    20161 ctggtaagtc aattgatttt gcttgatggt tagcaattgc ttcattgtaa tcgtcaatgg
    20221 ccattggttt tgaaagatct aaagaatcaa gtaatgataa ataaaatgct gagctttcac
    20281 tgcgatgcgt gtcttttcgt tcgtccattg ctgcgatgac agcagctttg attgcttcta
    20341 cactatcagt cactaattca tcaatgattt ttgattctac ccgttgacga gatcctaatg
    20401 tattccaaat caagtctttg tcactagaat caaattctcg aacgcctgct tcttgttcaa
    20461 taacttctgg tccattcaaa ccgactctcg ctttttttgt tgcgattaaa taactcatta
    20521 atgctgaagt aatcgacata ccgccaaatg atcctacacg tcctggaatc aaaccgatga
    20581 ctggtacata ttgttttaga gcaatgatta catttccgat ttctgaaata gataacagtc
    20641 cgtagtttgc ttcttgcaaa cgaacaccac ctgtatctag tacaatgact ggaaagatct
    20701 catttccttt tttattttct tctaacacat gttcaagtgc agcaacgatt tttgcaccgg
    20761 aaacttcacc gataccgcca ccttggaatt ttccttccat agcgattact actgcttgtt
    20821 tgccattgat cgtgccacgg ctgacaacga tcccgtcatc actttcaggg acgattcctt
    20881 gtgctggtaa acttggcgcg atgatttgat cgaatggatc aatcaattca cgtccattgt
    20941 caagtagagc gttcactcgt tctctggcgt ttaattctac aaaactattt ttcatctgaa
    21001 agttcctcca ttgcttgcgt caatcgtagg tacacaactc ctggtgtagc accgaagtca
    21061 ttgatcacga tatctgctga aattggataa cgatcaaaaa atctcgttaa tacattgttc
    21121 catacttcgc cgaagccatc gcttcctgta caaattttta cagtagattc tgttttttct
    21181 gttggaaaca tcaagatttc caaatctcct gaaccgacca ctcctacatg gattttctta
    21241 ttgatcggct gtttcgttgg atatttataa gctaattgtt ccatggatct ctcccctttt
    21301 tgtaaaacag agcatacacg ttctgcttat tcgtctttct tcttccatct gggtaaatgg
    21361 cgacaaaagg tttttctcgc caattttatg accaagttct gaatttcatt ggtggttcat
    21421 acaggccatc tgaccattcg atcaaatcat cgatggtttg tgcagctaat aatgaacgtt
    21481 tcgcttcact acgtttgatg cctagatctt ctggtaattt cacgatgccg gctgcacgca
    21541 agctatctag tgattctttc gtgctagtca ttccaagagg tgtaacaccg ccaattgctg
    21601 caattgctgc ttgtcgttct tcttttgttt ttgctttgta caaataagca attccttctt
    21661 ctgtcacgat atgtgtagta tcttcgctat aaatcatcac tggtgcattg gctaagcctg
    21721 cttgtttttg aacagctagt gcatctaatt cttcgacaaa gactggtttt ttatttgaac
    21781 catatgtttc aaccatttga acaactaatt tttggccttt ccctaatgga tcatcattgt
    21841 cgcgaagtga ttgccatgct ggtgtagaat gacgacgacc gccaggatta tgtcccatgt
    21901 taggcgcacc gccaaaacca gataaacgtc cttttgttac ggtagaagag tttccttcgt
    21961 aatctaattg caaggttgaa ccgataaaca tatcgatggc atattgtcct gcagcttgtg
    22021 ataatgttcg gttactgcgc atcgtgccgt ctttaccgac aaaaaagaca tctgggcgag
    22081 cagcaatata tttttccata ccaacttcgc caccaaaact atggatgctt tcaacccaac
    22141 ctgattcgat ggctggaatc atcgttggat gtgggttcaa tacccagttc ttcgcgattt
    22201 tcccttttaa ccctagtgat tcaccataag ttggcagtaa caattcgata gctgctgtat
    22261 tgaatccaat accgtgattc aaagattgta cgccgtgttc tgcgtagatt cctttgattg
    22321 ccatcattgc cattagaatt tgcaattctg tgatgttttg aggatctcgt gtgaataaag
    22381 cttctaattg ataaggttta tctgctttca cgacaacgtc tacccagtca ccagggatat
    22441 ctacacgtgg taaagaatcg acaacttcgt taacttgaac gatcacgata ccatctttga
    22501 aagctgcagc ttcgatgatt gttggtgttt cttccgtatt gaaacctgtg tacaagttgc
    22561 cgtctttatc tgctttatct gcagctacta aaacaacatt tggaatcaaa tcgatgaata
    22621 agcgtccata aagttccaaa taagtatgga tatcacctaa tttcattgtg ccgtcttcaa
    22681 tcatttgggc gacacgcaag ctttgtgcac cagcataaga gaaatcgatc ttgcttgcga
    22741 ttcctttttc aaaaatattc aaatgttctg gtcgtgagat actagacatg atcaaatgca
    22801 gatcattcac tttttctgca tctacttttt caagactttc tgaaagaaaa cttgcttgtt
    22861 tttgattgtc gccttctaat acaactttgt cgccgggagc aatcagtttt tccattactt
    22921 ctacgatttt ttcagtttct acatacttgc cattgagcaa gttgctgatt ttttccaatc
    22981 gctgcgcttt tttttcgcgt ttagaagccc agttgcggtt tggtttttcc ataattagtc
    23041 atctccattt ttgatataga aacgtggcgt catcaatacc atgttggtct agattgatcg
    23101 tacggatccc tttatctaca ccaaattttg tatgaatcag ctttgtcaaa gtattgccgg
    23161 ggggaaattc aatcgtcaga tccattccta attcttttgc caagtccatc atttggttcc
    23221 actgcacagg gaaagctagg ttttccaaca aatctttacg aatctcttct gcgtcctgag
    23281 tggctcttcc atccgtattt tttatataca aacatctagc atcagataga tggacagact
    23341 gcatatacgg cttgaatttc ttgaccgtat cattcatcaa gagacagtga gatggcacag
    23401 ggactttcag cagcttcgct gtttgcgctt ggtgccgttt ggcttctgcc aacgtaacgg
    23461 ataaaccttc gatactccca gataacgtat gttgcatcgg acaattttca tttgacagat
    23521 aaactggatg gtcaggatca aatgtttttg ctacaatttc ctcagcttcg cttctcgtca
    23581 cacctactac tacgcccatt gcataaccgc ttggataagc ttctttcatt aagactgcgc
    23641 ggtgatagac tagttccgct gcattctcaa atctcaaaga tcgacaagca acagctgcac
    23701 aaaaagcacc aatcgaatgt cctgctgcaa tatcaggctg aataccgttt ttttccattt
    23761 catctaaata aaatactgct tttgtcagta atgctagctg gataaaaacc gtatcttgat
    23821 agttttcttc tttatcaact aaccgatacc ccgtcacatt ttctaaacgt tccatgtaca
    23881 tttcagggat atccgctaac atccctaact tctgactgcc ttgtccagga aataagaaac
    23941 ctgtactcat gagcctcgcc tcctcagcac ttactttttc cactgctatc ttagtcaatt
    24001 tctgctataa taaaaaatgc caaaaaaaga aaaaaccata acaaaaaggt atggatggga
    24061 tggataatta tgaaattgct tcatctgcag tattttaaaa aagtattcga aacaaaaaat
    24121 gttacccaag cagccaaaga attatttatt tctcaacctg ccttgagccg tgcgatcaaa
    24181 catttggaaa acgaattagg tgtccctttg ttttaccata gcggcagaaa cattgagacg
    24241 accgtttatg cagaagaatt ttatccatat gcagtcaaga cattagacac attagaagaa
    24301 ggaatcaaat tattgtcttc ggtcaatgag aagatcgtta cttccgtcat attgtattta
    24361 gaggtagcct ctgtttctat tccgaacttg gtccgaattt ttatggaaaa gcatcctgat
    24421 atccaattaa cgatcatgca acatcattta ccagatgata ttaccgatcc tgtactgtac
    24481 atcacttcgg agatgaagcc tggcatgacc aatattccta tcatgaaaga acctgtttat
    24541 gtcgccattc ctaagaaaca tcctcttgct aaaaaagaac atctgcaatt agaagatatc
    24601 caacgcaccc ccatcttgat gctgtcaaag agcaacgcct tccgtcatac actggatgca
    24661 gcacttgaag cgaaaagtat cgaattgacg atcagttcga cgacagatga tcctgcgaca
    24721 ttacgttcta ttgtcaagca aggattagct gttagctttt tcccaaaagt ttcttggtct
    24781 tacgataaaa atgatccttt cgtcttgcgg gaaatcaaag atttgccctt gacacgaaca
    24841 atttatttat cttctagctt cacagaagat catcctttga tcaaaatgat tgcaaaaacg
    24901 atgcgggaat tttatttagg a
//
LOCUS       DAZUMV010000028        24891 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000028 DAZUMV010000000
VERSION     DAZUMV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.028"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            222..1988
                     /locus_tag="ACG8FK_001036"
     CDS             222..1988
                     /locus_tag="ACG8FK_001036"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS5096662.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2090..3349
                     /locus_tag="ACG8FK_001037"
     CDS             2090..3349
                     /locus_tag="ACG8FK_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5096663.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3496..4797
                     /locus_tag="ACG8FK_001038"
     CDS             3496..4797
                     /locus_tag="ACG8FK_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5096664.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4800..5651
                     /locus_tag="ACG8FK_001039"
     CDS             4800..5651
                     /locus_tag="ACG8FK_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5096665.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5664..6479
                     /locus_tag="ACG8FK_001040"
     CDS             5664..6479
                     /locus_tag="ACG8FK_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS5096666.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6630..7796
                     /locus_tag="ACG8FK_001041"
     CDS             6630..7796
                     /locus_tag="ACG8FK_001041"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5096667.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8026..8673
                     /locus_tag="ACG8FK_001042"
     CDS             8026..8673
                     /locus_tag="ACG8FK_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5096668.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8757..9701
                     /locus_tag="ACG8FK_001043"
     CDS             8757..9701
                     /locus_tag="ACG8FK_001043"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS5096669.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9836..11287
                     /locus_tag="ACG8FK_001044"
     CDS             9836..11287
                     /locus_tag="ACG8FK_001044"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS5096670.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11308..12144
                     /locus_tag="ACG8FK_001045"
     CDS             11308..12144
                     /locus_tag="ACG8FK_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5096671.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12144..12803
                     /locus_tag="ACG8FK_001046"
     CDS             12144..12803
                     /locus_tag="ACG8FK_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5096672.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12929..13618
                     /locus_tag="ACG8FK_001047"
     CDS             12929..13618
                     /locus_tag="ACG8FK_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096673.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13624..14580
                     /locus_tag="ACG8FK_001048"
     CDS             13624..14580
                     /locus_tag="ACG8FK_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS5096674.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14624..15136
                     /locus_tag="ACG8FK_001049"
     CDS             14624..15136
                     /locus_tag="ACG8FK_001049"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5096675.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15392..15940)
                     /locus_tag="ACG8FK_001050"
     CDS             complement(15392..15940)
                     /locus_tag="ACG8FK_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5096676.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16029..17210)
                     /locus_tag="ACG8FK_001051"
     CDS             complement(16029..17210)
                     /locus_tag="ACG8FK_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFS5096677.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17446..18954)
                     /locus_tag="ACG8FK_001052"
     CDS             complement(17446..18954)
                     /locus_tag="ACG8FK_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS5096678.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19113..19304
                     /locus_tag="ACG8FK_001053"
     CDS             19113..19304
                     /locus_tag="ACG8FK_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096679.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19507..20424
                     /locus_tag="ACG8FK_001054"
     CDS             19507..20424
                     /locus_tag="ACG8FK_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS5096680.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20417..21490
                     /locus_tag="ACG8FK_001055"
     CDS             20417..21490
                     /locus_tag="ACG8FK_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096681.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21638..22156
                     /locus_tag="ACG8FK_001056"
     CDS             21638..22156
                     /locus_tag="ACG8FK_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096682.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22137..22529
                     /locus_tag="ACG8FK_001057"
     CDS             22137..22529
                     /locus_tag="ACG8FK_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS5096683.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22605..23540
                     /locus_tag="ACG8FK_001058"
     CDS             22605..23540
                     /locus_tag="ACG8FK_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5096684.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23673..23963
                     /locus_tag="ACG8FK_001059"
                     /pseudo
     CDS             23673..23963
                     /locus_tag="ACG8FK_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            24054..>24891
                     /locus_tag="ACG8FK_001060"
     CDS             24054..>24891
                     /locus_tag="ACG8FK_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5096685.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDE"
BASE COUNT         8198 a         4405 c         4974 g         7314 t
ORIGIN      
        1 aaaattggag aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag
       61 actaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg
      121 gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgct tctctatcac
      181 ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt
      241 atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc
      301 ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct atgtggagat ccatatacca
      361 taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg
      421 ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag tcgcttacag tatgcgttcc
      481 atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc
      541 gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg
      601 atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa
      661 gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag
      721 atccagaccg agaagacttt ttcgggggag atttgcaagg tgtgatggat catttggatt
      781 acttgacgga ccttggcatc aatggcatct atttttgtcc catatttaaa gccacatcca
      841 atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac
      901 ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg
      961 tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa
     1021 aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga
     1081 ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca
     1141 tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt
     1201 attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc
     1261 atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc
     1321 ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga
     1381 tgaattatgc atttacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt
     1441 cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag
     1501 gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta
     1561 acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta
     1621 tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta
     1681 tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt
     1741 ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa
     1801 atgatcgtga agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt
     1861 actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc
     1921 aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca
     1981 tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc
     2041 attataataa gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag
     2101 aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc
     2161 ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt
     2221 ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga
     2281 aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc
     2341 gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg
     2401 tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga
     2461 tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat
     2521 cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa
     2581 agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa
     2641 tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta
     2701 cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc
     2761 aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg
     2821 aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac
     2881 tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta
     2941 tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg
     3001 aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga
     3061 ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc
     3121 aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat
     3181 tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac
     3241 aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc
     3301 agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata
     3361 accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat
     3421 ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt
     3481 agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg
     3541 ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc
     3601 gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca
     3661 ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg
     3721 attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat
     3781 cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg
     3841 atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc
     3901 gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca
     3961 tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct
     4021 aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt
     4081 tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc
     4141 acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc
     4201 ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac
     4261 gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta
     4321 gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta
     4381 gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg
     4441 atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt
     4501 tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat
     4561 acggggaatt tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc
     4621 gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa acttacgact
     4681 ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt
     4741 atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa
     4801 tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact
     4861 tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg
     4921 ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag
     4981 gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga
     5041 tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt
     5101 atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga
     5161 tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc
     5221 tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt
     5281 ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg
     5341 atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc
     5401 tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt
     5461 tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg
     5521 atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga
     5581 tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa
     5641 caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac
     5701 gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga
     5761 tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga
     5821 tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa
     5881 cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga
     5941 tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc
     6001 atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg
     6061 gtatacgccg ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc
     6121 ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca
     6181 ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat
     6241 tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat
     6301 tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag
     6361 ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg
     6421 tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac
     6481 aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg
     6541 aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta
     6601 atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat
     6661 tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat
     6721 acggagatac ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga
     6781 aagagattac aagtaacgct tctttggcac taggagaagc ctatatggat catcgaatcg
     6841 agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt
     6901 tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa
     6961 acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag
     7021 atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag
     7081 cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt
     7141 tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg
     7201 tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa
     7261 aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag
     7321 gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc
     7381 ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc
     7441 atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc
     7501 ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc
     7561 aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa
     7621 agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa
     7681 tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga
     7741 ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga
     7801 ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag
     7861 tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat
     7921 tttcataata aaagttcggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt
     7981 atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt
     8041 atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa
     8101 ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc
     8161 tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact
     8221 cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca
     8281 gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata
     8341 gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg
     8401 gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa
     8461 gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac
     8521 aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg tactacttat
     8581 tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca
     8641 ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat
     8701 ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga
     8761 gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg
     8821 tccactgcat gactacccag gaattagatg aactggctca ttcagaagca ggagcattta
     8881 tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg
     8941 ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag
     9001 agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca
     9061 ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt
     9121 ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag
     9181 cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc
     9241 cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag atggctgata
     9301 tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt
     9361 atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg
     9421 gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga
     9481 aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac
     9541 atctgctatg tggcgcaagc atgttacaga ttggtaccga gcttcataaa gaagggccgg
     9601 aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt
     9661 caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg
     9721 attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact
     9781 cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa
     9841 tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt
     9901 tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct
     9961 tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt
    10021 ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac
    10081 ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca
    10141 agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa
    10201 aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac
    10261 accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc
    10321 tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg
    10381 gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg
    10441 agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg
    10501 gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca
    10561 tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat
    10621 tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca
    10681 tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca
    10741 gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc
    10801 tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct
    10861 ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga
    10921 aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa
    10981 aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg
    11041 ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac
    11101 accttataca cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt
    11161 gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt
    11221 gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg
    11281 gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact
    11341 agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc
    11401 tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc
    11461 gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag
    11521 gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc
    11581 acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa
    11641 agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta
    11701 cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct
    11761 ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca
    11821 tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt
    11881 attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta
    11941 aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga
    12001 aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg
    12061 acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg
    12121 atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga
    12181 cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt
    12241 ttcttttagg aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc
    12301 ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc
    12361 tgctgtttct gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca
    12421 tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag
    12481 gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat
    12541 tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag
    12601 ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc
    12661 aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg
    12721 cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac
    12781 gttctatgca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc
    12841 tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa
    12901 atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc
    12961 ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc
    13021 tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga
    13081 atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc
    13141 atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac
    13201 tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat
    13261 gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc
    13321 ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa
    13381 gcaaatggag cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag
    13441 acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga
    13501 aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta
    13561 tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa
    13621 aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca
    13681 ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc
    13741 gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatccaat cgctgtgatc
    13801 caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc
    13861 aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta
    13921 ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag
    13981 gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt
    14041 cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat
    14101 gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac
    14161 gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa
    14221 gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata
    14281 cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt
    14341 cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt
    14401 acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga
    14461 gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat
    14521 ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga
    14581 ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac
    14641 aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc
    14701 ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa
    14761 cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc
    14821 tgcattagtc tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc
    14881 aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa
    14941 actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga
    15001 tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg
    15061 attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca
    15121 aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta acactactga
    15181 aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa
    15241 gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat
    15301 agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct
    15361 cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat
    15421 ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca
    15481 aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag
    15541 atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga
    15601 taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa
    15661 cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt
    15721 tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg
    15781 tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat
    15841 tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc
    15901 cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga
    15961 ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt
    16021 tttgtatatt ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct
    16081 gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc
    16141 ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca
    16201 gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata
    16261 gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct
    16321 ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg
    16381 aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa
    16441 ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc
    16501 aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct
    16561 tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca
    16621 agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata
    16681 gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt
    16741 aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca
    16801 ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc
    16861 ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca
    16921 attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa
    16981 acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca
    17041 gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa
    17101 acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa
    17161 gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc
    17221 ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa
    17281 attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca
    17341 aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg
    17401 attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag
    17461 attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg
    17521 ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag
    17581 tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa
    17641 gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg
    17701 tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg
    17761 ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt
    17821 aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact
    17881 ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg
    17941 acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa
    18001 tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc
    18061 ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt
    18121 aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg
    18181 aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa
    18241 ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag caatgttcag
    18301 cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc
    18361 atgtcctttt tggctttcat gatacagttg gttcaaggaa gtagcgagct ttccacttcc
    18421 tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc
    18481 aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg
    18541 tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg
    18601 tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc
    18661 gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg
    18721 ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag
    18781 caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc
    18841 aactagcttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat
    18901 atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt
    18961 ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg
    19021 aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt
    19081 ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg
    19141 catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg
    19201 aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc
    19261 atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt
    19321 catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta
    19381 ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt
    19441 tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca
    19501 agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg
    19561 aatgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca
    19621 agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa
    19681 gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga
    19741 agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt
    19801 ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac
    19861 aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa
    19921 atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg
    19981 gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt
    20041 gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat
    20101 aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag
    20161 caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc
    20221 cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc
    20281 gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa
    20341 gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat
    20401 gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca
    20461 aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga
    20521 tgacacagaa gaaatcccta tgctagagga aacaaaaata cacgaaaaag aaaaagtaac
    20581 tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg
    20641 gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga
    20701 agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt
    20761 attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac
    20821 aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga
    20881 acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt
    20941 gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca
    21001 agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga
    21061 tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca
    21121 gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt
    21181 taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga
    21241 atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc
    21301 ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc
    21361 tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta
    21421 cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag
    21481 aaagtcctga ttggataagt tggattacca gatgtcattc agataattct gttccaaagg
    21541 acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg
    21601 tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca
    21661 gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa
    21721 tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc
    21781 gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg
    21841 ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt
    21901 gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat
    21961 ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg
    22021 gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt
    22081 ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga
    22141 aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca
    22201 gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag
    22261 caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg ttcttcaaaa
    22321 aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct
    22381 tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta
    22441 ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat
    22501 cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat
    22561 tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa
    22621 aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca
    22681 aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta
    22741 tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac
    22801 agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg
    22861 caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga
    22921 cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt
    22981 tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg
    23041 aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt
    23101 ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga
    23161 cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc
    23221 tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg
    23281 tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc
    23341 tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag
    23401 atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata
    23461 tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc
    23521 caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat
    23581 aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct
    23641 gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc
    23701 ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt
    23761 gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc
    23821 tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat
    23881 ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa
    23941 gtcttgccgg ttttatgaaa cgccgattgt aaaattaagc tagacaacta aaaaagtcat
    24001 ttgtgctaca ctcaaaatag ttccgtccaa agaattataa ggagtgagta caaatgacct
    24061 atacccatct tacaacggat gaacttgtaa tgatagagtc ttatttcaaa ataaatcaat
    24121 ctgttgcgaa aactgcgcat ttcttgaatc gttcaagaca aacgatccat aaagtatacc
    24181 tgttcttcaa gcaaggaaaa tcagccttag agtactatca acagtataag aaaaacaaat
    24241 caaactgtgg tagacgcccg cttgttttac ctgaggaaca atcagaatat attcaaagaa
    24301 aggttgttca aggatggaca cccgatgtga ttgttggtcg tgcagcgttt cctattcgtt
    24361 gttctgctcg taccatttat cgtatgttca aaaaggggct atttaatcct tctgacttac
    24421 cgatgaaagg caagcgtaaa ccaaatggac atcaagaaag acgtggaaaa caagctttcc
    24481 gtcgctctat tcatgaacgt gaaaaagatt atagccaatt ctcaaatgag tttggtcacc
    24541 ttgaaggtga cactatcgta ggtttgaaac ataaaagtgc tgtaattacc ttagttgagc
    24601 gattatcaaa agttatcatc acattgaaac cgtgtggtag acaagcgatt gatattgaaa
    24661 acaaattaaa tcattggttt gaatctgtac cgaaaaatct attcaaatcc atcacttttg
    24721 attgtggaaa ggaattttca aattggaaac agatcagtaa tgccaatgat attgccattt
    24781 atttcgctga tccaggaacg ccgtctcaaa gaggcctaaa cgagaattct aacggattgt
    24841 tacgtagaga tggtttattg aaatctatgg atttcaattc agtagatgaa t
//
LOCUS       DAZUMV010000029        24754 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000029 DAZUMV010000000
VERSION     DAZUMV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.029"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(225..1007)
                     /locus_tag="ACG8FK_001061"
     CDS             complement(225..1007)
                     /locus_tag="ACG8FK_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096686.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1072..1908)
                     /locus_tag="ACG8FK_001062"
     CDS             complement(1072..1908)
                     /locus_tag="ACG8FK_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5096687.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1926..3335)
                     /locus_tag="ACG8FK_001063"
     CDS             complement(1926..3335)
                     /locus_tag="ACG8FK_001063"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5096688.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3349..5172)
                     /locus_tag="ACG8FK_001064"
     CDS             complement(3349..5172)
                     /locus_tag="ACG8FK_001064"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5096689.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6015..6341)
                     /locus_tag="ACG8FK_001065"
     CDS             complement(6015..6341)
                     /locus_tag="ACG8FK_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096690.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6387..6749)
                     /locus_tag="ACG8FK_001066"
     CDS             complement(6387..6749)
                     /locus_tag="ACG8FK_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS5096691.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6786..7001)
                     /locus_tag="ACG8FK_001067"
     CDS             complement(6786..7001)
                     /locus_tag="ACG8FK_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5096692.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7128..7463
                     /locus_tag="ACG8FK_001068"
     CDS             7128..7463
                     /locus_tag="ACG8FK_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5096693.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7539..9536)
                     /locus_tag="ACG8FK_001069"
     CDS             complement(7539..9536)
                     /locus_tag="ACG8FK_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096694.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9634..9909)
                     /locus_tag="ACG8FK_001070"
     CDS             complement(9634..9909)
                     /locus_tag="ACG8FK_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5096695.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10141..10875
                     /locus_tag="ACG8FK_001071"
     CDS             10141..10875
                     /locus_tag="ACG8FK_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5096696.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10914..12056
                     /locus_tag="ACG8FK_001072"
     CDS             10914..12056
                     /locus_tag="ACG8FK_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5096697.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12151..13542)
                     /gene="sufB"
                     /locus_tag="ACG8FK_001073"
     CDS             complement(12151..13542)
                     /gene="sufB"
                     /locus_tag="ACG8FK_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS5096698.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13547..14017)
                     /gene="sufU"
                     /locus_tag="ACG8FK_001074"
     CDS             complement(13547..14017)
                     /gene="sufU"
                     /locus_tag="ACG8FK_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS5096699.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14004..15239)
                     /locus_tag="ACG8FK_001075"
     CDS             complement(14004..15239)
                     /locus_tag="ACG8FK_001075"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS5096700.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15236..16522)
                     /gene="sufD"
                     /locus_tag="ACG8FK_001076"
     CDS             complement(15236..16522)
                     /gene="sufD"
                     /locus_tag="ACG8FK_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS5096701.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIVVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16535..17308)
                     /gene="sufC"
                     /locus_tag="ACG8FK_001077"
     CDS             complement(16535..17308)
                     /gene="sufC"
                     /locus_tag="ACG8FK_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS5096702.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17505..18353)
                     /locus_tag="ACG8FK_001078"
     CDS             complement(17505..18353)
                     /locus_tag="ACG8FK_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5096703.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18377..19063)
                     /locus_tag="ACG8FK_001079"
     CDS             complement(18377..19063)
                     /locus_tag="ACG8FK_001079"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5096704.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19056..20090)
                     /locus_tag="ACG8FK_001080"
     CDS             complement(19056..20090)
                     /locus_tag="ACG8FK_001080"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5096705.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20153..20409)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20485..20823)
                     /locus_tag="ACG8FK_001081"
     CDS             complement(20485..20823)
                     /locus_tag="ACG8FK_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS5096706.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20825..21181)
                     /locus_tag="ACG8FK_001082"
     CDS             complement(20825..21181)
                     /locus_tag="ACG8FK_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS5096707.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21187..22374)
                     /locus_tag="ACG8FK_001083"
     CDS             complement(21187..22374)
                     /locus_tag="ACG8FK_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5096708.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22565..23410)
                     /locus_tag="ACG8FK_001084"
     CDS             complement(22565..23410)
                     /locus_tag="ACG8FK_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5096709.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23644..24609
                     /locus_tag="ACG8FK_001085"
     CDS             23644..24609
                     /locus_tag="ACG8FK_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS5096710.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7220 a         5214 c         4214 g         8106 t
ORIGIN      
        1 taaaaatttt ttcactaaaa tcactacctg aaacagtcta aaacacttat atatcaacgt
       61 ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg ctccaaatcc
      121 gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag tacggtcata
      181 taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc aataactttt
      241 ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga ataatctttt
      301 ttcccatttt caagtaaccg aagtggaatg tctttaccaa cttgctctgt cactgcatta
      361 ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt agaaatctct
      421 gtatcagtat ttatcgtgat tcctttatct tcatttacaa gcttccaagt aggtggtata
      481 gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc agtatctact
      541 tctgtctcat tactcttaga tgaatcgatt ctttcatcca tcgtctgctc cgtaactgct
      601 tctgtctcat tattctcacc ggaaactctc ttcacaccca aacttatata ttgtccatct
      661 ttagtgtgca tagttaaaat tcccacgctt ttactaataa attcactata accagttctt
      721 tcaatagaga cttcaatgta tttcacacta tcatactcaa aagaaaaata gacatgtaaa
      781 agcattttgt tgtttacatc tacataataa tcctttgact ctacttgaga ccttcctata
      841 cggaatacag ctttactgtc tgatatctct ataccagcat ccttattatt aatctgccaa
      901 tctccaagta gctcttctgg caagctagta gtttcttgtg aacactttac cacttcactg
      961 gtagaggtcg aaacattcac ctccgcactg ttagaagaat tttccaagga atcaggagcc
     1021 ttagcacaac caacaagtct ttatatattg tgcagccgat attttcttca ttcagtacac
     1081 ccttgaaata tgcagtgtta gataaagcag ttcgtcctga tttgctgagg cacccatctc
     1141 ttcttgaacg tactccttga ttttcaaggc cgcccgatac tctttcggaa aacgctgctt
     1201 cactgaagcc agcaaaaaat catccttctc cggcaactct tccttcttaa tcattcgggc
     1261 aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt tatcaacctt
     1321 tttttcggga aacagatatc ctagcatagc caagatatcc tttagtgctc ttaccgagag
     1381 catggtttct tccatatttg cttgttcact ttgggcattt acaatgtgaa acgcaatgtt
     1441 tccagcctcc tcttctggca actgaacatt catttttttg ttgatcaatt ggatagcata
     1501 gcagccgatc tcaaattctt ttgggtaata cttttgaatt tcaatcagca ggcgattttg
     1561 aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa ccaaggtaaa
     1621 aaatagactt ttattcagct taaagtcaaa cttggcgttg gcataatcga taattcgctt
     1681 gacgcaaata attagatcat ccggagcagc ctcaaacagt ttaatgtaat ccttttttag
     1741 agcatcagaa agtgaatcta atttaaatac tttttggatt ttgtcagccg gaaccaaatc
     1801 gcctttcttt agttgaaatc caatcccttt ccccatcaaa attacttctt tgccttgctt
     1861 atctaagcaa agaattaaac tgttgtttaa cgttttaatt actctcatta gattcaccgt
     1921 tctctctaag ctaagccatt tcttgcgatt actttttgat accaaaagaa gctttctttt
     1981 tttattcgtt ttaattcttt aagattctca tcggttcggt caacatatat aaaaccataa
     2041 cgcttattga agccttgatg ggtactaaca acatcaatcg ctgaccaagg taaataacca
     2101 aaaacatcta caccatctgt taaagctgct tgaatagcca gaatatgttt ggctagataa
     2161 tcaattcgat cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg tgccccatag
     2221 ccattttcag taattaaaat aggtaaatca tagcgatcgt acaatttcct caatgttaat
     2281 cgtagtccag tagggtcaac tacccagcca tatggcgttt tatctacata aggattttca
     2341 gctgcccggt agacaccttc ttctcccaac atgatctgtt gatcccccgc tctagcggaa
     2401 acatctgatc cgtcattttt gctggcagcg atggtggcag ttgaataata attaatcgca
     2461 ataaaatcgg gtttaccgga ggataaaatt gcctgatcct ctgcctcaat tttaggatat
     2521 aatccacgat cattcaaata gttttcaaac aaataattgt attttccccg aactgctaca
     2581 tctaagaaag accagcctct caatgtttcc caattatggg ctgcaattgc atcttctgga
     2641 ctggaggtag cttgatacat agccgttaaa ttgattgctg gaccgatttt tcctttaaga
     2701 ttcaactgat gatacaagtg aattactcta gcttgagcta ataaaacatg gtgattaatt
     2761 tgaaagagtt ctttttttga aggcagctct ccacttggtg gcaaaccaat cgctccagga
     2821 tgaaggatca tcgtgttttg ctcattaatt gtcagccaat agttcacctt gtcaccatac
     2881 tcagtaagca atacctgtac atagtccaca aaataatcga tagttgctcg atttaaccaa
     2941 ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt acatggtgac aatcggttcg
     3001 attccctgat tcaggcattc atcaattact tgatgataaa aagaaattcc cttttgattg
     3061 gttttattat tttcaatatc cataattcgc gtccaagcga tggaaaagcg aaaagctttt
     3121 aagcctaatt ctttaaacag ctgtatatct tctttatagt gatggtaaaa atccgaggca
     3181 acttcaaaat tagctacccc ttctgtatgc tgatacttgt caataataga cggttgtttc
     3241 ccatcctcct gataggcacc ctctgcctgg taggcactaa tggaggcacc ccataaaaac
     3301 ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt aactaatttg
     3361 catcaatggt tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg cgtaattttc
     3421 actgttggtc actaccacaa tcactgtatc atcgtagcca gcgtttctaa tggcagcgaa
     3481 atcggcgttg attaataatt gtcctgctga cacctgatct cctgctttta caaatacttc
     3541 aaagggttcc ccttttaaat tcacggtgtc gatccccaca tggatcaata attcaatacc
     3601 ttctttagaa cgtagaccaa tcgcatgttt agattctaaa atagtaatga cttctccctc
     3661 aacaggtgaa taaatgctgt tttcttctgg atgaatggct acgccctttc ccaatagtcc
     3721 cttagaaaag acagcatcgt tcacttcaga aataggacac acggttcctt ttaccggatt
     3781 ttcaacaatg actgcttcgg ctattgccgt ttgctctgat tcatcagaag tatcgtcaaa
     3841 tccaattaga taagcaatga taaaggttgc aacaaaggcg attacacacg taataattgc
     3901 gtgaacaata ttcattggat tttctccaat aaaggctggt aaggctgcta agccagggga
     3961 aacaaaggca tatctgacta aaccgctcaa gccagcatac aaaccagcaa ttgcaccacc
     4021 aatcatggaa gcaactaatg gttttttcag cttcaaggtt actccataga gtgaaggctc
     4081 agtaattccc attaaagcag tgatcccagt ggacacagct agctgcttca actctttgtt
     4141 tttagttttc gcggctacag caaaggtagc acctgattga gcaatattag aagccagcat
     4201 tcctggccca ttgatcattt cagaacccgt actgctcaat tgcatagtag cgattggcgt
     4261 cattgcccaa gctgtacccg taaccaccaa aaatggctgc aaaccaccca tcaaaaatgg
     4321 aattaaccaa gctgctttgc cgttaatcca ttcagccccc attgctataa gatcgttcag
     4381 caacgttcca aacggtccaa tgacaaccaa cgctaaaggc cccataatta aaaataccag
     4441 catcggctta gtaaagaatt taataaacga aggcgaaatt cgatccgcaa agggctctac
     4501 ataggacatg atccaaacag taaagataat cggtaaaact gatcccgcat aatctgcagc
     4561 taataccgga attcctagaa aagaaacggc atcgccagta gaaaaaagct gtgaaatatt
     4621 tggatgaagc aaaatgccag caacagtaat cgccaaaatt gggttgacat caaactttat
     4681 ggctgcccca taggctaata gtactggcat aaaataaaag gctgcatcag caatagtatt
     4741 caaaatgatg taagtttgac tactgtctga tagtacacca gtcaaagtca agacggccaa
     4801 aatggctttt accatcccgg cacctgttat agctggaatc atcggtgtaa aggtggtaga
     4861 aatgacacta atcaatcttg aaataatact ttcttgattg tctgaagagg ggcccttttt
     4921 aattgaagtt cccacttcct tttgaatttc cttgaaggct gtttggactt cattgccaat
     4981 caccacttgg tattgtccac cgctatccac cacactaatt acaccaagtg ttttttctaa
     5041 ggctttcgta tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg tggcacaatg
     5101 ggttaagctg accacatttt cttttccccc aacctgttgc aatacatttc ttcctacttc
     5161 tctgtaatcc attattctct ctccttaaag cagtaaaaaa gaccaagtca aaccacgccc
     5221 caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt aacatgccaa
     5281 taacgttatt ttgctgtaat ccaataatag cacattatga tagcgcaatc aacagggaat
     5341 ttaatttttt atcttttcgt ttgaccgtat agggtaatat aacagttaga tcacatcaac
     5401 tagggtatat ctgcgtatac ttactgctaa aataagtata tctaatatta actataccag
     5461 taaaaccatt cctacataac cagcgggaaa ttcccaaaat cgtgaaagca tcaaaaatgc
     5521 atataacgga tcacacaact tcaacaattc tgctcttggg aaaaattgat gaatttggtg
     5581 agtaaaggat ggaataaaca gcattccaac aatataggca acaatagtga tacttgtttt
     5641 tttggtaaaa atcagtaagc aatagccaat ttcaatcatt ccgagatata aaggtatcga
     5701 tagaatgatt tgggctagta ttcttaaaaa ttaactagca aatgtttaaa ctgatttatt
     5761 tctaatctac atgtagtaag tatttcaatt ccaccaatta tcaataaaaa taccaaacat
     5821 atccataata attttttctt tttcatttct tttatattgt tataacagtg aataacataa
     5881 aaattcccga caatcattgt aatatttttt tgtcacctgt attagtattt aatttatagg
     5941 aaactataaa aactccctag tgaatttaca cctttgggag ttcctgtttg gacggtatgc
     6001 ataatacatt taaatcaatt taacattaac acctcagcat atggtatctc cacatatata
     6061 cggtctttct ttggatagta ctcaatattt atgttgatag cacttccatt gtctaatata
     6121 gctttagccc ggtatgtgtt atctccattt tcatgagtaa tcgtcacatc atcacatgat
     6181 cgatttaagt tgtactgttc ttgtaaaatc tgtgttacca atggttttga tgacttctct
     6241 aatccagatg tacctgtcat ttttgaaaaa ataataccta taatagccaa gacaacaata
     6301 cctataatga caaacttctt gttcatattt actttattca actttttatc tcctatctcc
     6361 aaaataattt gctcttatcc ctcttatcag tacattgaca tccctaaaag tacaccaaag
     6421 attgctccag ctacccctgc tatcatcaaa atcgtaccca ttttctcatc tttttctcga
     6481 tataagtagc ccataataac agcaacagca ccaaatacaa caggaataaa tgctaacgat
     6541 actaccatag aaatccaacc aataatcaaa tacagcttcc ctgtctcttg tccatgtacg
     6601 atttgttcct tcatttcatt aattgtctgc tcagtacttt tctttactgt gtctgttccg
     6661 gttgtctcat ctgtttcttg tagtgctcca caaaactggc aatacttact ttcatctgtt
     6721 atttctttgc cacattttct acaatacatg tcttctcctc ctaaaatcat tcaattgctt
     6781 attagttaac tgtttacttt attaaataca tcagaaaaca gctcattcat tggtacactt
     6841 aatatctcag aaagcatctt catttgatag ggtttaggaa tcgttctccc tgtttgccac
     6901 gaacttacac attgctgtgt aacattcatt ttttttgcta actccgtttg agaaagtcct
     6961 ttttcttttc tgtatcggct gagattactt acttttttca tgattctccc ctcctgtttt
     7021 attatataca acaatatgat gtatttcaac tcataaaata taaaaaactt caaataccat
     7081 tacaaataca acaacttgtt gtactatact gagatggggg tgtattaatg aaacagaaac
     7141 aatatgcaaa ggtcattaga gaaaagcgaa aagctctcgg gtggacacaa aaagaattag
     7201 ctgaaaaaat cttctctaca caacaggcag tagcaagatg ggaaaattct gtaacagagc
     7261 ctaacctcga tagcttaaca gctctttcta gagccctagg aacccctgta agccattttc
     7321 tagacaatgt tgtcgtagat tatgaggaag agtttttggc tctctatcgt tctcttagca
     7381 ctgaagatgc agttcgaaca attgactata tgaagctatt aaaaaggcaa gaaaatgaac
     7441 gcaatcagct attgaaagat tgaaaaataa ataacccacc gaactttaac ggtgggtgta
     7501 ttgagatgaa ataagattaa gagctatgga taacctgtct agtttaatac aattgggtct
     7561 tttctcttcc ttactctccg tccatcatct cttgttacac ttgtaaattg atacagagga
     7621 actatcccag ctcctgataa tatcaattct tttattgttt catctatccc tgcccctttc
     7681 gctaaatata ttaaaaaact actacttgct ttcacattgt atagtttctt aatatcctta
     7741 gaagctcgtt caagagtgaa tccttgttta acaaagtagc ctctgttttt tattaaagca
     7801 tttttcacgc tgttatcccc aacattacgt aggcgttcac aaacagtgtt aataagttct
     7861 ttctctgtca ttagatatgt cccttgccac gataagccct gtaaaacttc atgtgcttca
     7921 tctaaaatca attgtttttc cttgctgagt tctgatcggg aattttgagg aaatactctt
     7981 ttagcctctt tttcgttaaa acatatctta acccattcat aagaaaaata ttgatatgta
     8041 aaatgaattt tttccagaag tggaaggata tcttcattca atttttgaat cacagaagtt
     8101 gcattaaaaa cgtctaatcg atagacattg cttctcttat gattaaagtt tcctaacgct
     8161 ttctcttcct ttaacctatt taggtattcg agcatcttca aaggaacatc ttcatcatct
     8221 aattttttta tcaatcctat aaaagtcata agtgatagta acttattaca tttatctatg
     8281 tcataagttt taccatagta agaactcatt tttttagcta attgttcccc cgacaaaata
     8341 caagtcacac tttccttact atcatcaaaa aaattaacat cagaaagcaa gacatcatag
     8401 acacgtcgca tttcctctag ataattcctt attgtcctga aaagtttgtc tttcattgat
     8461 tcaagagtgt caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg tgtaggatac
     8521 aagtctaggt tctctaatag ccaattaaat gtttctaccc gactgtcttt ctttaaaatt
     8581 agcatcacaa ctccaataaa attttcactg atatcacaat tactggaatg acaataaaaa
     8641 atggaatgcc cagttttact ggtaaaaata ctagcagagg gattatgatc gttgtgaaac
     8701 agacatctta agtttttact tgacttaagt aatttattca tatcaatggt taacaatctt
     8761 tcataaatct cattaaaaga catgtcggag gaatcaaaca ggcagtcttt aaaccactgt
     8821 ttcatttctt ctttgtcgtc attttttatc aacttaacaa aatcggtttc attgactagt
     8881 tcatactctc tccctgaatc actttgacaa tttttagaac atgtcatggt aaaatcaatc
     8941 ggaagctcag taatatctaa catgttgtct tcattaatta ttatcactgc atctgactta
     9001 tatcctccaa aaaacattct attcgaagca tttacattgt catcacaccc ggggatgttc
     9061 tttttcaagt attcgtaaac agcaaccatt ttaccatagt cagtaattgc ttcttttaat
     9121 agaaagacaa tcttgtattt ttctagattt tcagagtacc gaatactttt ttgtatcgca
     9181 aacgcagaat tatgaactaa attgatagat aaaacctttt ctacgcttaa gtatctacta
     9241 tcatctttac tcaaatctac taccttcccc ttctcatctt taacagtgtt atcgacatcc
     9301 agaataatta ctctggtttc cacaaaactc tccttgtcat tgcctttatt ctttgtcttg
     9361 cttggaataa atgattgtga tgaatttagt atctctacaa actcatctct tgtaacttct
     9421 ctatagttat ctaaacacct atctcttaca tcagtttttt ctttttttgt aactggcttc
     9481 tctaataatt tttcttgatc tacataaatt ggaatattaa tcattgatgt ttccatttgt
     9541 gtttcctcct tataaaattc tgttaaagac taaaacatac agataataaa aacaaactct
     9601 ttattactat atgttttagt cttatttgtt tactcaacct ttataccgtt ccatgaaact
     9661 atcaatatca gattttttaa atctaatgac cgtatcaatt tttataacag gtaacccgta
     9721 ttgagaaaca tattttctaa tcgttcctac tgatacacct agatattcac ttgcctgctg
     9781 atagtccata tactctagag tgactccaga aataatgtct tctagcatag gctttagcac
     9841 tgtaattatc tctttcactt gttgttcaac ttgtatagaa acaatgctat caatgatttg
     9901 tagagacata tttccccctc ctttcgtgat tgcttgaaca aattttctaa gaattatctt
     9961 cgtatttttt gctcaaaata aaagtatttt gttattaacc gattaatcaa acaaaaaatg
    10021 attaccagtt catcgacata cacataatac ctcgataaaa aaggggtgtc aacatctttg
    10081 taaaatttat ttacaacacc ccttttttga ggtatactaa ttttaggagg tgtggtcaat
    10141 atgataaaac gtccaccaat aaattattta gaacgaaaaa agatactcgg aacaaaaata
    10201 aaagctataa gaaagagtaa aaaattaaca caaccagcat ttggtcttat gattaacaac
    10261 ggtcaattaa ttgacaaaaa aactatctac gaatgggaga agggaaccta tttaccaata
    10321 ccagagcgat tatcccgaat tgctgatttg ggaaatatga gtatagagga gttagtttgt
    10381 ggcaatgtag aagaatatat tttgggaatt attctttata gagatagcat tgtactagat
    10441 ggtataacat ttccagataa aaacctgttc cagcatcttc gtcaacagtt cccaccagta
    10501 cactctaatt tagatacatg gctggataga tattccaaat tagaaccaga gatgcaagaa
    10561 tttattgcta acaagacttg taataaagtt aaaaatgaaa aaattagtct tttcaatatc
    10621 ttaaaaatag aagaattatt catcaatgct attgtagaaa aatttgacaa taacattctt
    10681 ttcctcacta gcagtataga agaactacta gaaagaatgg tagatgaatg gctacctatt
    10741 caattgaaag acatgtctta tcccgaagaa gctgtgagag aaattactga taatataaac
    10801 aagctagaac aaacgatttc atcaattggg aaaaaataca ctaagaaaaa aatgaaggga
    10861 ggtgatacca tctagtggta agtactcctt gtaaactaag aggagatgat ttaatggcaa
    10921 aaatatcaaa gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa ggacacattg
    10981 ccaccgatcc acgaactggt gaaaaaataa atactacgag aagaggtttt agtagcaaac
    11041 aagaagctca aaaagattat gacgagtaca gacatcaaat attatacggt gttaaaaaga
    11101 aagcacctga tatgacattt gaagacctgt ataatgaatg gataactcat cagcgtacca
    11161 gtgttaaagc ttcaacaatc gcaatctcag taagatacgc aaataatcaa atacttccag
    11221 catttggtaa gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa gtggtagatg
    11281 aatggcacag taaatatgaa agttacgatt atatgagaaa acagactgca caaattttac
    11341 gctacggggt tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca cttcttccac
    11401 gaaaaaaaga gtatgagaaa aatcgtaaat tttactcaaa agaggagctt aataatttac
    11461 tggatgcttt taaagatttt ggtaacatga aacaatacgc cttcttcaga ttattggcat
    11521 acacaggtat gagaaagagt gaagtactcg ctttacagtg gaaagacata gacacgttta
    11581 ataaagaact acatgtcaat aaaactcttg ctgtagatga atttggtaaa gttataattc
    11641 aatctccgaa aacaagagct tctagacgag ttatctcttt agatactgag actctttcta
    11701 tcctcaacaa ctggaaatta cagcaaaaag aagagtattt aaaacttgga tacaatacta
    11761 gctctaaaga gcaacatgtt tttacaacag ttaaaaacac tctatacatt ccaaacactg
    11821 ttaatgattg gttaagatat attttgaaaa aatataactt gcctcgtatt acacctcatg
    11881 ggtttagaca tacacatgct agtttattat tagaagcagg agaatctgtc aaggtagttc
    11941 aacaacgatt agggcatgaa aatagtaaag tgacattaga catctatgca catattacaa
    12001 ataatgcacc taagaaaaca ggtcaagact ttgctgatat gatggctcat cagtaaaaga
    12061 gctggtcaaa atctggtcat ttctagtata caggcaaaac aaaaaagcta gaacccttat
    12121 atatcaaggg ttctagctcc tataacaggt ttacccaaca ctgccttcca tctcataact
    12181 gatcaatcga ttgagttcga ctgcatattc cattggaagt tctttggtga atggttcaac
    12241 aaagcccatg acgatcattt cagttgcttc agattcggtc aggcctcggc tcatcagata
    12301 gtagagttgt tcttctgaga tttttgagac tttggcttcg tgttctaaag agacttggct
    12361 gttgtggatc tcattgaatg ggatcgtatc ggatttcgac cattcgtcca tgatgatcgt
    12421 gtcacattcg atatgagaga tcgaacccgc actgtttttg gcaaaggtta cttgtcctcg
    12481 atagtttact tctccgccgt cttttgaaat tgatttagag acgatagagc tagatgtatt
    12541 tggtgcattg tggatcattt ttgcacccgt atcttggatt tggtttgctc ctgcaaaggc
    12601 gatggagagc atggtgcctc gtgcaccttt tccatctaag taaacacttg ggtatttcat
    12661 cgttgttttt gcacctaggt ttccgtcgat ccattcaacg gttgcgcctt cgtatgcttt
    12721 tgctcgtttg gttactaagt tgtacacatt gtctgaccag ttttggatcg ttgtgtaacg
    12781 gcaatacgca tcttttcttg taaagatttc tacgatggcg gcatgcaggc tgttacttga
    12841 ataagtaggt gccgtacaac cttcgacata gtggatgctt gctccttcgt caacaatgat
    12901 cagcgtacgt tcgaattgcc ccgtgttttc cgcattgatc cggaagtacg tttgtaatgg
    12961 aacatcgact ttgacggctt ttggcacgta aataaaggtc ccacctgacc atacggctga
    13021 attcagcgct gctaatttgt tgtcggttgg cggtacaagt tttgaaaagt actctttgaa
    13081 taattcaggg tattctttca aagcagagtc agtgtcagta aagacgattc ctaacttttc
    13141 gaattcatct ttcatattat ggtacacgac ttctgattca tattgtgcgg aagcaccggc
    13201 tagataagct cgttcagctt ctggtatacc aatcttttca aatgtatctt tgattttttc
    13261 tggtacgtct tcccagtcac gagcgggttt gtcacttggt ttttggtaat atttgatggc
    13321 atcgaaatcg atgtctgaaa ggtcggggcc ccatgtttgc attggcattt tgttaaatgt
    13381 ttctagagat ttcagacgga attcaagcat ccattctggt tcgcctttga ttcttgacat
    13441 ctcacggatc acttcttctg tcaagccttc tcctgtactg aaaaccggtt cgacatcatc
    13501 gtgaaacccg aatttatatt cttctaattc tggtacgccc atgttctcac tcctcttcct
    13561 tgtggtaatg atggaaagct gcggtaccat cgttttccat tgcttttcct agtgctttcc
    13621 aggcaagtgt cgcacatttg atccgagcgg gaaatttagc tacaccacca agcatagcag
    13681 catcgcctaa ttggtcggga tctttgactt cttttccttg gacaagctgt agaaagtcta
    13741 cagttaaatg ttctgcttct tctattgatt ttccgatgac ggcgtctgtc atcatcgagg
    13801 cacttgccgt actgatggag cagccactgc cgtcaaaggc gatatttttg atgattccgt
    13861 cttttacatc gacttgcaat tcgatcacat ctccacatgt ggggttgttc aattcgatct
    13921 tgcttgtcga gtcagtcaag cttccacgat gatgcggatg ggaagaatga tccaagatca
    13981 cttgacggta taaattgtct aatttagata gtcccatgtt ggaaaaactc ctttgttgcc
    14041 atgattgctt caactaaacg gtcggcgtct tgcgttgtgt tgtagaaata aaagctcgcg
    14101 cgagcagtcg ctgatacttc aaggtacttc aatagcggtt gcgcacaatg atgacctgct
    14161 ctgacggcta ccccttccat gtctaaagct gtcgcgacat catggggatg taacccatcc
    14221 aaattgaatg caacgactcc tgtccgaaaa ttcgggtctt gtggaccata aacagtcaat
    14281 ccatcaattg cttgtaattt tggcaacaca taagcggcta gttcttcttc atatcgatgg
    14341 atattgtcca taccgagttt ttgcaaataa tcgattgcat ggcctaatgc gatagcgccg
    14401 gcgatatttg gtgtccctgc ttcgaatttc caaggtagtt ctttccatgt actgtcgtat
    14461 aattcgacaa aatcgatcat ttcgccacca aattcaacag gttccatttc ttctaaccat
    14521 tttcgtttac catataaaac accaatacct gttggtccgc acattttgtg tccgctgaaa
    14581 gcaaagaaat cacagtctag tgcttggaca tcaatggcca tgtgcggggc agattgagca
    14641 ccatctacta cgatgacccc gccgttttca tggacaagtt cagctaattc ttgaacagga
    14701 ttgaagacac ctaagacatt ggatacatga gcaatggaga cgattttggt tttgtccgta
    14761 atctttttcg ctgcatcctc catatccagg aagccttctt ctgttagttc gatatatttc
    14821 aagacagcac ctgttcgttt tgccaactgc tgccacggga taacattaga atgatgttcc
    14881 atgtaagaga gcactatctc gtctcctgct ttgatatagc ggtcgccgaa ggttctggct
    14941 acccaattca agcttgtcgt cgtaccacga gtaaacagca cttctgctgt ttcttttgca
    15001 tggatgaacg cccgtacttt ttctcttgct tgttcataat catgggtcgc gcgttctgct
    15061 aacgtatgga caccgcgatg aacattggcg ttatcgtgtt cgtaatagta cgctaatgcc
    15121 tggagtactt gttttggctt ttgggtcgtc gctgcattat ccagataaac taaaggttca
    15181 tcattgacgt cttgaaataa gataggaaaa tcattgcgta gctttttagc atcgatcatc
    15241 cattcaactt cccttcgatt acttcgacta attctttccg aacttgttct acagggattg
    15301 ctgttaagac agaaccgagg aatcctcgaa tcactaagcg ctctgcttct tctttatgca
    15361 atcctctact catcagatag tacatttctt ctggatcaac tcgtcctaca ctggcagcat
    15421 gtccagcagt tacttcgttt tcatcgatca aaagaatagg attcgcatca ccacgtgctt
    15481 tatcagaaag catcaagaca cgactttctt gttgggcatc tgcacctttt gcccctttca
    15541 agatgtggcc gataccatta aaggtcaatg ttcctttttc tcggatcact ccgtgttgca
    15601 aaatatgccc gatcgtatga ggggctttat tggttactcg tgtatcgatt ccttggattt
    15661 gtctgcccga gctgattgcc acgattttga cttctgcgtg tgcaccttct cctgctaaat
    15721 ctgagtcaaa gtcagcgatc acatgaccgt cattcatgac aacaattgcc cagtcgacag
    15781 aagcatctcg taaaatgtgc ccacggcgat tcatataaga agtaatattt tctcctaatt
    15841 gatctacggc tgaatatttg atttttgaac cagctttagc gatgacttcc acgatgatat
    15901 tacctgatga ttttgctact tgttctttgg ttgtctggaa acgttccaga taagaaaatt
    15961 cactatgttc atctgccacg atcaacacat gcttgaagaa gtgctcatcg gatgcgccat
    16021 cttgaatgaa taaggactcg agtggttcat cgatcacgac atttttaggc acatacagga
    16081 aaataccgct gttcataaaa gctacatgga gtgcagtcaa ttggtcttcg ttcatgttta
    16141 ctgctttttt catataatat tcttggacta gttctggata ttcgatcatt gcagtgaaca
    16201 agtcggtaaa aatgactcct ttttctgcta attccacagg cagttgttca aaaatcgtcc
    16261 atgaaccttg ttggacaagc acaggattgt ctttcattgc atcaaatgcc ggaatcgtgc
    16321 ctgtttcact gatggtttgt gtttctttca catcaaataa cgaccagcga tggaatttca
    16381 cccgatcgat atgcggcaat ggcagttcat ctgctttcgc tagtgcgtct tgacggaagg
    16441 ttctcatcca atcaggttcg cctttactta aagaaaaatc atttaccgcg tcaagataat
    16501 cgagccagtt tttttctttc attcttgctc ccccttacgc ttcttcttct ttatagtcga
    16561 ttcctaattc tttactgata ccagcgtagc cttctgcttc cagacgttta gctaggtcag
    16621 cattccctgt catgactact cggccttcca tcatgatgtg aacgacatca ggtgtgatat
    16681 agttcaataa tcgttgatag tgggtgatga tcaatgcacc gaaattgtct ccgcgcattt
    16741 catttactcc tttagcaacg actttcaaag catcgatatc cagacctgaa tcgatttcgt
    16801 ctaaaattgc aaatgtaggt tctaacatca acaattgcag gatctcattc cgtttctttt
    16861 ctccgccaga aaaaccttcg ttcagataac gttcagccat ttcttctggc atatttagta
    16921 aagccatttt ttcatctaac tttttcaaaa atgccataac agaaattttg tcgttttcat
    16981 cacgtttcgc attgatcgct gcacgcatga attccgcatt cgtgatacct ggtatctcac
    17041 ttggatattg catagctaaa aataagccaa gtcgcgcacg ttcatctact tccaagtcta
    17101 aaacattttt cccgtcaaac aaaatttcgc cttctgttac ttcatagttt ggattgccca
    17161 tgatagcggc agataaggta gattttcctg tgccgttagg tcccatgatg gcatggattt
    17221 ctccggtttt cattgttagg tttacgcctt taaggatttc tttttcctcg atcgacacat
    17281 gtagattttt tatttctaag acagacatgc attatacccc caaattctct ttcgttctca
    17341 ctcattttag actaattgag aatgaagcgc aatgaccttc cttactaaaa tcaaatatct
    17401 tttatttatt attaaacaat tttgatttct aaattagaat aggtataaaa aagatgagaa
    17461 ataagccatt acagacttat ttctcatctt ttttgttcca taccttattt tgcatcagcc
    17521 tttactggtt taacagagcc gccccatttg tctgttatcc attcttggac ttctttgcgg
    17581 tgaagcactt caatcagttt tttgattttg tcactgtctt catcaccttt acgagtcgca
    17641 ataatattca cgtaaggaga gttgtccttt tcaagcaata cggcatcttc taatggattc
    17701 aaccctgcgc cgtaagcgaa gtttgcgttg attgcaacta ggtcaccttc atcattttgg
    17761 tacgtagaag ctaaaagtga tgggtcgatc gtatggttga acttcaagtt cttagggttt
    17821 tcttcaatat catcgaatgt tgctgtttcc agatctactc catctttgac tttgactaag
    17881 ccagcatctt gtagaattgt gatgatgcgt ccccagtctg attcagaatt tgaagtaatg
    17941 attgttgcac catcttttaa ttcggaaaca tctttgactt ttttagagta caagcccatt
    18001 ggttcgatgt ggacagcacc ggcattgaca aagtcatatc ctttttcttt gatttctttg
    18061 ttcaaataag gaatatgctg gaagtaattg gcatcaatgt cgccttcttc caaagattgg
    18121 ttcggtaata catagtcatc gaatttgacg atttcaagat cgattccttc atctttcaat
    18181 aatggtttca catgttccaa aatctctgcg tgcggtgtcg gtgaagcgcc gactttcaat
    18241 gtcgtgtcgt ctgctttatt tgctgaatct ttgctgtcgc tgttgttacc gcatgctgct
    18301 aatcctacag tcagcaaaag cgctgctgca aatccaaaaa ttttcttttt catatcctac
    18361 atccccctat atcctattat cttttgtctg ttttcttagt taagaaatca ccgattcctt
    18421 gaacgataaa tacgattagt aaaataatta aagtcgcaac gaatgtcact gttagattac
    18481 cacgctggta gccttcctgc caagcaagac ctccaagtcc acctgcgccg atcgcgccgg
    18541 ccatcgcagt aaatccgatc atcgagatcg ttgtaactgt taatccagaa atcaatgcgg
    18601 gaatactttc aggtatcaag accttccaga tgatttccca gtaacttgct cccattgcat
    18661 ctgctgcttc cagtacacca gggctgactt cacgaaaagc aatctctact agacgggcat
    18721 aaaatggtgc tgcggataaa acaagagctg gaatggctgc ttgcgcacct agcatagtgc
    18781 ctacgattgc tttagtaaat ggaatgatca aaaccatcag gatcataaat ggcgttgacc
    18841 ggaaaatatt actgatgatc gatacgattc cataaatgat tcgagcatac ggtttgtttt
    18901 tgcgtccaat agagtaaagg atcaatccca aaactaatcc gatgatcgtc actaagacca
    18961 ttgagattaa tgtcatatat agtgtatcaa ggatagcctg attcatcgtt tccgggttga
    19021 tttgtttaaa atctagatat gtttctgaaa agcttttatc cacgatggat cacctcaatt
    19081 cctactcctt gttctttgat gaacacttct gcttgatcta attctttggt ttcaccagtc
    19141 atcaagacag tcaatgaacc aaaggaacct tctttcactt gtttgatctt tccatacaca
    19201 acgttgacat cgatgttata gcgtcgaatc gcttgggaaa tgactggctc gttggcattt
    19261 ctttcgctaa aggtcaaagt tgccaaacgg ccgcttggat tttctttgat gagttcagct
    19321 aatagttctg tcgaatcagc ttctggttcg atgtcttgtt gtacaaagcg tttggttact
    19381 tcttttttcg gttgtctgaa gacagtcaat acgtctcctt cttctacgac tttccccaac
    19441 tccatcacag cgactttgtt gcagattttg cggatcacat tcatttcatg tgtgatcaag
    19501 acgatggtca aattcaattt cttattgatc gctaacaata agtctaatac ttcttctgtt
    19561 gtttgcgggt ccaatgcgct agtagcctca tcacaaagca ggatctcagg atcatttgcc
    19621 aatgctcgtg cgataccgac gcgttgtttc tgtccgccgg aaagttgtga aggatatgct
    19681 tgtccgcgtc cttctaaccc gactaaacgc aacagttctt ctgcccgttc tttacgtttg
    19741 ctctttggta cgccagctaa ttccagcggc agctggatat tttcaagtac agttcttgac
    19801 cataacaggt tgaaatgctg gaagatcata ccgattttct ttcggaatgt acgtaattct
    19861 ttgtttttta attttgtaat atcttgatcg tttacgatca cttcgccgct cgtgggaagc
    19921 tcaagtccat tcaggagacg gactaatgta ctttttcctg cgccagaata gccgacgatt
    19981 ccataaatat cccctttttc aacgtttagt gaaacgtcat tgacggcttg gatgtttccg
    20041 tttttcccat tgaatgtctt tttgacattc gttagttgga ttaatgacat gccattcaca
    20101 tctttctctt atgatttttc aaacaaaaaa gccttcgccc tcttgcctta agcaaaagga
    20161 cgaaagcttg aacttccgtg ttaccacctt gatttaccgt tacttcgcaa taacagcctc
    20221 ttccagtact tggacttagc ttctttccgt atactgtcgc gctgtaacgg gcactcccgt
    20281 agtaagtttg cttacgctcg cttactctgc tctaagacca tcttccatct cattttcgtt
    20341 acccattttc agcaaacagg gctctcttca aacgtccgtg acatgtactc ttcttttcaa
    20401 agctttctcg tttcatatag gattgcttat gattatagca actggaagaa tcagtgtcaa
    20461 cttttttcaa gtgattttct gattttataa ttcgtcaaat tcttttggat caacatcttt
    20521 gaaacttaag atccacgcat tcatttcatc tgaatcattc aagatattga tatcttcacc
    20581 gattttatca ttaacagaag aaactgtacc cgttagcggg ctggcaaatt cattgaccgc
    20641 tttttccgct tcaacttcga tcagaggttc atcttttttt agcgattgtc ctgcttttgg
    20701 cagattggca aacgtgatgt ctccaagttc ttcttgcgct tcatttgtca aaccaacaca
    20761 gtattctttt ccattaaata agatccaaag gttgtccttt ttcttcaaat gcttgcctgc
    20821 cattttatat cagttctcct tcataaattt tttcattgaa tccaattgca acaagctggt
    20881 cctctcgtaa gatcagtggt cgcttgatca gcataccatc tgtcgataag acttggctcg
    20941 cttttgaagc cgtaaactcg cctacttgat ctttcagtcc aagttcccga tatttcatcc
    21001 cgcttgtgtt gaacaggcga cggattggta cttgactcgt ttctagccaa gtctcaatcg
    21061 ttgaggcctt cggtacttct ttgatcatat caacggtttg ataactaacg ccatgctggt
    21121 ctaaccacgc tttggctttt ttgcatgttg aacatttcgg gtaccagtaa aaggtgtaca
    21181 attttcttac ctccttgaag atcttgcagg tgcttcattt tggtatctca tcgataatat
    21241 caacccgata ccgatcatgt tccctaaaat cgaggatcct ccctgactaa tgaaaggcaa
    21301 tgggatccct gttaaaggaa gtaatccgat atttgctcca atattctcaa agacatggaa
    21361 cagcagcatc atgatcaaac cgcttgcgat ataggcgtaa aattcattat tcgtatcgaa
    21421 acacacccga atcatacgat agattaaaat aaaatacaag aaaatgacga atgcaccacc
    21481 gataaagccg aagttttcac cgataacggt aaaaatcata tcggactcac gaacaggcac
    21541 atagatattg cttacgttga atcctttacc aaacaagcca ccggtaccaa ttgccaaaag
    21601 tccttgggcc ggctgataac tcattcctga cgtatcatgg aaagggtcca gccatgaatc
    21661 aatacgtgca aactgataag atttgaatcc aactttataa agcaagtctc ttccgccttc
    21721 agtcgtaacc aagaagatcg ttcctgctcc aacgactact gccaagatta ctacaggtgc
    21781 gatgatgcgc caagagattc cagacatcag gaaaatcccg ccaaagatcg caagaaagac
    21841 aagcatcgtt ccaaagtcat cttgggccat gatcaatgct aaaaccggta ttgtcacgat
    21901 caagagcttc ccaatcagcc aaaagtccga tttcatcgtc cgttctttta cttgcgtatt
    21961 atgtttagtt acgatcagtg ccatcatcaa gatgtacgca atcttcatca actctgctgg
    22021 ctggaaagta aacgcaccga atcgaaacca gtttttagac cctgttgaag cagctaaatt
    22081 ctgatcatag aatttgagca agagtcccat taccactaac cccaaaccat aaaggtaagg
    22141 agtcagtttc cagatccatt tggaattcaa atgcatgatg acaacgattg ctgccgctcc
    22201 aactaaatac cagacaactt gcatccctac gcctttcaag actgctggat tgttcggatc
    22261 atggctaagt gctacatata atgagagcag accaatcaaa cataacagaa agactggtaa
    22321 aatcacgcca taatcaatac gattatcgtt cgaagctttt aatttttttt ccatgtttca
    22381 tcccttttct taaaaaccat actgctgatt attatagcgg tccagttaga taatggaaag
    22441 tctttccata gacatttggg aaaaattaac tttccgtttg tgcagttgct tgagcaaatg
    22501 cagtacaaaa gcctactgct tatctttagt gtatcataga actttttttg atacatgcaa
    22561 ttatctacca gctgagattc tcctcgctgt gagaagtttc tgtgctttca ttgtatcgaa
    22621 ggatctcttc ggcaatctct agcagctgat cagaaaccag attggttcgt tggatgaaat
    22681 aaaaatatcg atggaaattc aacggagtca ttggcacatt ttctggaaca gagcgcttag
    22741 acaaaatcga gcgaccgatt ccttccttca gcaacgcaac aattacatcg ttacttttca
    22801 cgatcatctt cttatcttgg atattcatct cttcaaaata acgctgcgtg taatgaaaaa
    22861 caccagagtt tgcttctctt acaagccaca atttgctttt tgggtcccca gcgatgacta
    22921 gctggtcttc catcaaagag tatctttgta cgccggaagt ctctaatggc ttttcgataa
    22981 aaccaaaatc ggattcatgt ttcgctactt gttctaatga ttcatgagaa ttcgtgattt
    23041 cgatctcaaa atgcacttta ggaaaccttt tgatcagatg tttcatcaac tgaggcaaaa
    23101 gatagacagc aaaggtgtgg gaagcaacga tccgacatac ttcttcttca ttttgttcat
    23161 tgagcaattt ttgctgagtt tcttcccatt cttctaacaa aaccagtgtc cgttcatata
    23221 ataagtcggc ttgcttagtt ggaagcatct cttttcttcc gttacgttga aaaagatcga
    23281 tattcaattc ccgctccaac tgtttgattt gattcgagac tgctggttga gataaaaaca
    23341 gtacttctgc tgctctggaa aaatttcttg tttcatatac tgctcgaaaa gtcgttaata
    23401 acttaaacat ggcaccactt ccataacttt ttatgataat tactataaaa actatttgat
    23461 ttaaatatac aaaaaaccat ctataataga aatgtacaac aaaacagttg ctgatccaac
    23521 tagtaactta gatgaagaca atcagctgtc tgcttgctcg taatcataca ttcctattca
    23581 ttttacaatg tattcgttca tcttggtact aaaatctatt aaataaaaag gagaatatta
    23641 tatatgaaga actttttgaa aattattccg atcccgatct gcgggttgat ccttgggtta
    23701 gcttcccttg gaaatctgtt taaagactat caccatgttg ctttaggcaa tctaactggc
    23761 ggtatctcta tgattcttat gatattcatt tttgttaaat tgtttatctt attcgaacac
    23821 acgaaacaaa cgttgcatga tccaatcatc gcctccgttt caccaacttt tacgatgtca
    23881 ctcatggtta tttgtactta ttttgtttca tatgaaagta ttgcaccaat tgtcaaattc
    23941 atttggctgt gcgctgttat cttccaagtg atccttgtct tttatttcaa ttatcaccat
    24001 gttatcaaag ctgatctttc aattgaagca atttatccaa gctggtttat catttacgtt
    24061 ggatttgggg tcatcactgt aactgccggg aacttcttcc cacttattgg aaaaattttc
    24121 ttttggattt cattgatctg ctatgcgttc ttattgccga tcatcatcaa acgtgtcttt
    24181 ttcgtaagaa atatggctca tccgacacgt ccgctgatta cgatcgttgc agcacctggt
    24241 tctctttgcc taacaggtta tcttaaaaac tttgctgaac ctaattttta tcttgtcgta
    24301 gcattgttcg ctttatctca agtattgtat ttcgttgtgc tgtctatgct tccacgtttg
    24361 ttcaagcttc gtttttatcc aagctatgca gcctttacat tcccgcttgt tatctctgca
    24421 acagctatat ttactactgt tcattacttc aacagtttag gtatctatag tagcgtattg
    24481 gatttcttaa aagttgcgga atgtctgttt gcaagtgtag ttgtcttcta cgttcttgct
    24541 cattacgtac gtttcttaat taaagaacat agcaaacatg gaaaatttgt ttctaaaaaa
    24601 atcgcttaat aatcgattca ataggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    24661 cggccaaaca aaaacagctg ttcatgtttt tgtactggtt gggcttatta ccgcagatgc
    24721 aagcttttga acaccgttta ccaagaggtc gtaa
//
LOCUS       DAZUMV010000030        24260 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000030 DAZUMV010000000
VERSION     DAZUMV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.030"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..845)
                     /locus_tag="ACG8FK_001086"
     CDS             complement(<1..845)
                     /locus_tag="ACG8FK_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5096711.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDS"
     gene            complement(1069..3009)
                     /gene="thrS"
                     /locus_tag="ACG8FK_001087"
     CDS             complement(1069..3009)
                     /gene="thrS"
                     /locus_tag="ACG8FK_001087"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS5096712.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3346..3525)
                     /locus_tag="ACG8FK_001088"
     CDS             complement(3346..3525)
                     /locus_tag="ACG8FK_001088"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS5096713.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3572..5395)
                     /locus_tag="ACG8FK_001089"
     CDS             complement(3572..5395)
                     /locus_tag="ACG8FK_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5096714.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5673..7952
                     /gene="helD"
                     /locus_tag="ACG8FK_001090"
     CDS             5673..7952
                     /gene="helD"
                     /locus_tag="ACG8FK_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS5096715.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8045..8692)
                     /locus_tag="ACG8FK_001091"
     CDS             complement(8045..8692)
                     /locus_tag="ACG8FK_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096716.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8871..9590)
                     /locus_tag="ACG8FK_001092"
     CDS             complement(8871..9590)
                     /locus_tag="ACG8FK_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5096717.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9721..10872)
                     /locus_tag="ACG8FK_001093"
     CDS             complement(9721..10872)
                     /locus_tag="ACG8FK_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS5096718.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11089..11829)
                     /locus_tag="ACG8FK_001094"
     CDS             complement(11089..11829)
                     /locus_tag="ACG8FK_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5096719.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11833..12156)
                     /gene="rsfS"
                     /locus_tag="ACG8FK_001095"
     CDS             complement(11833..12156)
                     /gene="rsfS"
                     /locus_tag="ACG8FK_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS5096720.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12213..12806)
                     /gene="yqeK"
                     /locus_tag="ACG8FK_001096"
     CDS             complement(12213..12806)
                     /gene="yqeK"
                     /locus_tag="ACG8FK_001096"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS5096721.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12790..13440)
                     /locus_tag="ACG8FK_001097"
     CDS             complement(12790..13440)
                     /locus_tag="ACG8FK_001097"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS5096722.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13450..13761)
                     /gene="yhbY"
                     /locus_tag="ACG8FK_001098"
     CDS             complement(13450..13761)
                     /gene="yhbY"
                     /locus_tag="ACG8FK_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS5096723.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13777..14886)
                     /gene="yqeH"
                     /locus_tag="ACG8FK_001099"
     CDS             complement(13777..14886)
                     /gene="yqeH"
                     /locus_tag="ACG8FK_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS5096724.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14883..15410)
                     /locus_tag="ACG8FK_001100"
     CDS             complement(14883..15410)
                     /locus_tag="ACG8FK_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS5096725.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15590..16375)
                     /locus_tag="ACG8FK_001101"
     CDS             complement(15590..16375)
                     /locus_tag="ACG8FK_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS5096726.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16368..17237)
                     /gene="accD"
                     /locus_tag="ACG8FK_001102"
     CDS             complement(16368..17237)
                     /gene="accD"
                     /locus_tag="ACG8FK_001102"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS5096727.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17238..18617)
                     /locus_tag="ACG8FK_001103"
     CDS             complement(17238..18617)
                     /locus_tag="ACG8FK_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS5096728.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAKQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18625..19044)
                     /gene="fabZ"
                     /locus_tag="ACG8FK_001104"
     CDS             complement(18625..19044)
                     /gene="fabZ"
                     /locus_tag="ACG8FK_001104"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5096729.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19069..19545)
                     /gene="accB"
                     /locus_tag="ACG8FK_001105"
     CDS             complement(19069..19545)
                     /gene="accB"
                     /locus_tag="ACG8FK_001105"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS5096730.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19548..20783)
                     /gene="fabF"
                     /locus_tag="ACG8FK_001106"
     CDS             complement(19548..20783)
                     /gene="fabF"
                     /locus_tag="ACG8FK_001106"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS5096731.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20802..21539)
                     /gene="fabG"
                     /locus_tag="ACG8FK_001107"
     CDS             complement(20802..21539)
                     /gene="fabG"
                     /locus_tag="ACG8FK_001107"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS5096732.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21551..22468)
                     /gene="fabD"
                     /locus_tag="ACG8FK_001108"
     CDS             complement(21551..22468)
                     /gene="fabD"
                     /locus_tag="ACG8FK_001108"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5096733.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22575..22799)
                     /locus_tag="ACG8FK_001109"
     CDS             complement(22575..22799)
                     /locus_tag="ACG8FK_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5096734.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22852..23814)
                     /locus_tag="ACG8FK_001110"
     CDS             complement(22852..23814)
                     /locus_tag="ACG8FK_001110"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS5096735.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23824..>24260)
                     /locus_tag="ACG8FK_001111"
     CDS             complement(23824..>24260)
                     /locus_tag="ACG8FK_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5096736.1"
                     /translation="PDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIEAI
                     GMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGKLL
                     FRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6630 a         5410 c         4234 g         7986 t
ORIGIN      
        1 ttcgaatcca taaaatattc ttggattgct tctcgtgttt ttttcacttt ttcgctaagc
       61 ttttcgtttt tagccatata gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa
      121 agtgaattag ttgccgcact tcgattcgtg atttcttttg gtctagatgt ttgtacgagt
      181 acatcggcta tcttgctaag cgggttattt ccaaattgcg tcaacgcaat gatttcgata
      241 ttcatgcttt tcgccagctc ggctaatgcg actacatctg cacttctacc tgagtttgaa
      301 acaagccaaa gcactttctt tttctcattg ctgacaagtt gcgggagcaa gacgtggata
      361 tctttttcaa aaatgattgg cttaccgaca cgagaccatt tttgcagaat atcttctact
      421 actaaagaag aggcgccaac cccatataaa aaaacagtct ctgccgattc caaacactct
      481 acgacttttt ggatcgtttc ttcttctaga atatctactg tttcttttat cgctacttgt
      541 gcattgttac caagtttgtt tttgatagag taaaaggact catttgcttc tatatcacta
      601 aaaccttcat cgattccagt ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga
      661 ctttgaaaac cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct
      721 tctgctaatt cattagctgt cattgtaggg accttctcaa tattaccgat aatgtagttg
      781 gccaactttt tttctgctga agaataatct tccatcatac tttcaatacg actaattacg
      841 ctcataagtt cctccaccct tttaatgata gtctatgtaa gtataccata tcagaccctt
      901 attcacggtc gtatactgga taaaaaacaa aaaaacaatc cgcccaacag aatatcttat
      961 cccatcaatc ccacagattc tattgagcat ctgtgtcttt gaggaatacc tgttatctat
     1021 tgttcggatt gctttctatc tactactttt ttacaggaaa tttacctatt agttttctct
     1081 gctgtaattt ttgacttctg cttccatagc atcaataaag atattcaaat cttctacagc
     1141 tgtttcttta cttccataac gacgtacgtt cactgttgca tcttccaatt ccttgtctcc
     1201 aacaactaat tggtaaggga tcttttgtgt ttgagaggca cgtatcttat agcccatttt
     1261 ttcattacga tcatcaactt ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc
     1321 ataagcataa tcgccatgtg catcaacaga aacagggatg attgttgctt gtataggtgc
     1381 caaccatgtt ggaaaagcgc ctttgtatac ttctgttaaa taagcgacaa aacgttccat
     1441 tgtagaaaca attccgcggt ggataactac tggacgatgc gtattttccc cgtcttctcc
     1501 aacatatgtc aaatcaaagc gctctggcaa taagaagtct aattgtatag ttgataatgt
     1561 ttcttctgta ccaagagctg ttttgacttg aacatctagt tttggtccgt aaaaggctgc
     1621 ctcgccttct gcttcaaagt aatctagttc taattcatcc atagcggctt tcagcattgc
     1681 ttgggctttt tcccacattg cgtcatcatc aaaatattta tctgtattgt ttggatcgcg
     1741 atagctcaag cggaaacgat aatctgtaat attgaagtca gcatatactg ccaccatcaa
     1801 ttcaagcgta cgtttaaatt catctttgat ttgatcagga cgaacaaatg tatgaccatc
     1861 attcaacgtc atttctcgta cacgttgtaa acctgacaaa gcacctgatt tttcatagcg
     1921 atgcatcatt cctaattcag cgatacgaat tggtaattca cgataagaat ggatcgtgtt
     1981 tttgtagacc atcatgtgat gagggcagtt cattgggcga agaacaagca tttcgccatc
     2041 tcccatatcc attggcggga acatgtcttc ttgataatga tcccagtgtc cagatgtttt
     2101 gtacaactca acatctccca ttattggtgt atatacatgt tggtaaccta gactaacttc
     2161 tttgtctacg atataacgtt cgatcgtgcg tcggattgtt gccccttttg gaagccagaa
     2221 tggcaaacca gaacctactt ctggggaaac catgaacaag tcaagttctt ttcctagttt
     2281 acgatggtct ctttcttttg cttcttcacg gagacggata tattctttca aagctttctt
     2341 gtcaaaaaag gctgtcccgt atacacgttg catcatttga ttgtttgagt ttccacgcca
     2401 ataagcacct gccactgata ataatttaaa tacttgtata cggccagtgg atggaacatg
     2461 tggtccacga cacaaatcga tgaattcgcc ttgttgataa gcagtaatca cttcttcttc
     2521 tggcaattca ctaatcagtt ctactttgta cggatcactt gcaaaaattt ctaatgcttc
     2581 ctgttttgaa agaacacggc gttcgatcgg cagattttct ttgacgattt tcatcatttc
     2641 tgcttcgatt gccggcaaat cttctgcagt tacttggttt tgtccattat ctgtatcgta
     2701 gtaaaaccct gaatcgatag ctggtccaac gccaaagtgg atatttggat agaggcgacg
     2761 catggcttgg gccatcaaat gagctgctga atgacggact aagccaagtg catcttcatg
     2821 gtctggtgtg atgatctcaa tacttgcatc ttcctcgatt ggacgagtca aatcaacgag
     2881 ttttccgttc actttaccag ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc
     2941 aattgccaat gttgttgttc cagactcaaa ttctttgact gcaccatctg gaaaagtaat
     3001 ttttatcata atagtttcct ccttgaaatg ttttgattga tcagatcatc aagctgaaaa
     3061 acaaaaaaat cctagcatct cttgaaaaga gatactagga cgaaaagttc gtggttccac
     3121 ctaatgttca aacgcaaatg cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc
     3181 aacaagggtt tcgaaagtgg tcatcctttg gttttctcct ggaaaagttt cagcctagct
     3241 tttcctctct tatcgaagac tctcaaattc agttggcttt ctcatcaatc aattatgtgt
     3301 ttatttaacc actaataaat agaaaaatca agtgggaaac taaacttatt ttttccattc
     3361 cccatttaca ccgtatgttc cttttttcat ctcattgatg atgacatgga tgtgttcttt
     3421 tggtgcctgc gtgtttttat gaacagcttc cgtgatatct ttcatcatat tttctaattg
     3481 ttccggactg cgaccttcta ctaattcgac atgtacgaat ggcataatgc tctctcctct
     3541 ttctgatcgt tcttttttca actcgattct atcatactat gtttgtcttg ggtactgaat
     3601 caggaaaaat ggctggatct ttttttgctc tcaaggcact caaaatagag accgtatcca
     3661 cgacttcttg caaaacagct ccgaataatg cggggattag tcccgtactt gcaattaaca
     3721 tcaaaaaaat acaaataaag atgccaatca gtacagactg tttcgctatt ctcatcgtat
     3781 ctcttgaaat cactactgct tttgctacac gcaagagatc atctttcaag atgaccgcat
     3841 ctgcactttc actagcagct gtagaaccat gagcccccat tgcaataccg atatctgcag
     3901 cagcaagtgc tggtgcgtca ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt
     3961 tagggatact ttctaaaatg gtcagtttat cttgaggcaa gctacgggca tgtacagaat
     4021 ctaattggac tttttctgca atcgtttgag caatggcaga tccatccccg gtgatcatca
     4081 tcaaattttt cacatcaagt tttcttagct ccttgattgt ctttgctgct tctgggcgaa
     4141 tctgatccga aaataggatc gttcctgcaa aagatcgatc aattgttaca tagattgctg
     4201 tattggacgt ttctatttct tcagctgaaa caaaagaggc acgtccaatc tttacttcat
     4261 gattattgac tactgcttgg attccttgac cggtcacttc tttcagttga ctaacaggat
     4321 acagttggct gtttttcacg ttgttcacta acgaacgagc taagatatgg ctagattcct
     4381 gttctgcact agctgcatac agaagtagct cttctttttt tattccattg actggctgaa
     4441 tcgcctctac ttccaatatc cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt
     4501 ttgtacgtgc gagcttttca atcgttgttc cagtttttac aacgatcccg tttttacttg
     4561 aacggctcat tcctgcaact aaagcaatgg gcgcagctag aatcagtgga caaggtgatg
     4621 ccactaccag tacctcagca aatcgtaaag ggtcttttga gaaccaccaa gaaataccac
     4681 cgatcaaata agcaatcaac gtgaaaggga ctgcataacg atcagctaaa cggacaaatc
     4741 gggcaggctt ttcttttgat tcttcgacta gtttaataat catttgatac tggctgtctg
     4801 cggcacgttt ttcagcgatc atttggatcg atccgtctcc atttaaagac cctgacataa
     4861 tctgatctcc aggtttcttg gtttccgggc gggactcacc tgtcaaagaa gattcatcga
     4921 ccgaagattc tcctttggtc actcgaccat ctgcgggtac aagttcgcca ggcttgacta
     4981 gtaactggtc atcaatattt atttcatcga ttactatatc ctgcaatttt ccttctttga
     5041 atcgatgggc aattcttggg gtgttatcta acaaagactg cagttcttta cccgctcgtt
     5101 tattcgcgta atcctctaga ctatctccgc ccgtaagcat gatcaatatc atcaaactag
     5161 cccagtattc tttgacggct aatgttgcaa ggattgcggt aatcgctaat acatctacgc
     5221 catatcttcc tgaacgtaac gtcttgatca tttccaaaaa catcaagaag gcgagaatcc
     5281 ctccagtaaa tgcgacaatc gcaaatgcaa gggccggctg gtgaaaaaca aattcactta
     5341 ataaagctat cccaccaata agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt
     5401 tttctcctct tttatgtgac ttagagaggt tagtaataaa tttccagctg cctctttcat
     5461 cttaagcata gtccttgact agcacttttt aaaacaatag gccttaattg aaaacaacta
     5521 tcactatcag cgtttccaaa attcatcagt tctacaaaca aaaatattca gcaaggatta
     5581 ctttttcgat tcatcagtta aaaagtacct tttttcgaaa aactatgcta tacttttaat
     5641 tcgttattta cggcggaagg agcaaacatg cgatgaatga gaaacaacta gaacaaacgc
     5701 acgtggatga aacgatcgaa ctaatccaga tcgaacaaaa tatgctggat acaaaacaac
     5761 aagaattgac tcaggaaatg aatcattcga caaaagacac agctaatcac aagattcgag
     5821 ctggttccaa tgaagctttt tatgaatctg ctgttgaata tcgccaacat gagcaggaat
     5881 tgcttttgaa ataccatact cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca
     5941 tgaaagatag tccttatttt gcccgtatcg actttaaaga agaccgtgaa acagaaacat
     6001 tatatctagg tatcgcttct ttaagagatc tggcagaaga aacaatcgtt attgactggc
     6061 gcgcaccaat tgccaatctt tattatgaag gtgagattgg tcccgcattt tatgaaactg
     6121 aaacagatag aatagatgtc gatctgcagt tgaaacgaca atttaaaatc caagaaggac
     6181 gtattttatc tatggtagat acgtcggaag taatcaatga tgactttttg ctagagatat
     6241 tggatgaatc atcaagcaac catatgaaaa acatcgtctc tacgatacaa aaagcacaaa
     6301 atgcaatcat tcgggacaca acaagtaaag taatgctgat tgaaggaatc gccggaagtg
     6361 gaaagacatc cgctttgctt caacgtattg cttttctgtt gtatcataat cgcaagtggc
     6421 tcgaggctga aaacgttctt ctattttcac ccaaccattt gttttctgat tacatctcga
     6481 ctgtattgcc ttcattaggt gaaagcggcg tgcctacaca gacattcaaa aattatttgg
     6541 tgcagctgct gccagagttt tctttactag atgaacagca acaagaaagt ggttttttgc
     6601 taggggaaaa agatccgata caagtgatga aaagcggatt gaccttagtc gatcagattg
     6661 atcgctatat ccaaggcatc acaaactacg gtcccttgtt ccgtgatatg aaaataaatg
     6721 gccgtacgat cctatcaaag gaatcgattc gccaatggta taaagaaaca aatgaacaat
     6781 tacctttaca tcatagactt tctctattac aaacgaagct cctgaaaaaa ttaggcggtc
     6841 tgcaaaaaga cgaaacacgt cagcaatggg tcaaagatct ggcggaagaa cagctccaag
     6901 aactatacgc aacagatcca aatcttgaat atacagaaca aaaggagcgt tcattacgta
     6961 aaaaactggc caaccaaatc gtacgtaaaa gattccgtcg aatccatcga gggatcatga
     7021 attatcaatt tgtcaatata acgaaacaat atctccactt tctacaaact gtgccacttt
     7081 ctcttctaac acgacataaa atcagcgaga ccaggtggca ggaacatttg acaactgttc
     7141 gtacacactt gcgtcaaaga gaattgcgtc aagaggatgc tgtactattc tttttactga
     7201 tgcgccgaac atatccagtg acggttacac acaaagctcg ctatatcttt attgatgaaa
     7261 tgcaggattt cgcaccatca caagtggcat tgctgcaagc cttgttccca aaagcaaatt
     7321 tgactttctg tggagatcta aatcaaaatg tctttggaaa tgaaacgatc gttggatcat
     7381 tggaagacct tttcgtggac cgtgaagtca ctcgtttcca attaacgaca agctatcgct
     7441 ccacaaaaga aatcactgat tttgctaatc attttattgt gaaagaacat caagtagaaa
     7501 caacagcaag aaaaggagac cttcctttgc tcatccaagg aacaacagat gatatcaaat
     7561 tgaattggtt agcagatacc attcgaacga ctcagggaaa agcccggtat tggcgtacag
     7621 caatcatagc taaaacacaa gaagaatgcg aagctcttta tgctcaactg ccagacgagt
     7681 tgaaacaaca agtccagctg attttaaatg agagtgactt tatgaagcgt tccattattt
     7741 tacttccagc ctttttagca aaaggtctgg agtttgaccg agttttcttg tgggatgtag
     7801 atgcagaaca tttttcttct cagcaagaca aattgatcct ttacaccatg tgtacacgtg
     7861 cgatgcatga attgaccctt tttacctcca ataatgaaag tcctttgtta aacaatatgg
     7921 atatttcatc ttatgagaaa aaagtactgt aagtaaaaag acaactgcta tcattaatgt
     7981 tagcagttgt ctttttattt acaataataa ttgcagaaaa agtaattcaa tcatttaagg
     8041 tcatttattc tatatataaa tccaaatctg tttcaggata ttttaatttg acaagcgaaa
     8101 taatcatttt cagttcttcc atgttttctg gatattctgg gtcaaatacc ttgctatgac
     8161 ggccaatccc gccattgtcg atcaatcggt aaattcgatt ccattcaatg aattggtcaa
     8221 ttgattggaa attgctgatt ggatagccgc cacaatccaa aatctctgat acacgaaatg
     8281 ctaggtatgc actatcgatc actttatttt ttttcgtgtc tatcttctca tttctttcga
     8341 taagaatttt ttcaggacga tgtttcaagc tccactctaa tagtagaaag aaagcctcca
     8401 tccgatactc ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa
     8461 tcagataatt ttgtctataa atcagcattg gtgcgcggtc caccatacac tctaaataac
     8521 ggttcagata ccctgtaccg acatagtttc ctaatcgata gccttcaacg ccgaatcttg
     8581 cgtatttttg cgctaatttt gaacaatcag ttacgttcac agctaactct tctactttgc
     8641 ggtaaccgct taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc
     8701 ccgtccatct tccacatact tcatatcatt tcacatgata cttatgctct catcatacca
     8761 caggttgaat tagaaaagaa aataaatcac acaaaaaagc aagacaatct gttagtgaga
     8821 ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa aactggttgc ttagtcttct
     8881 aatgccacat tatggaacac atgctgtaca tcttctagat cttccagtgc atcaatcatt
     8941 ttttcaaatg tttcttgatc ttctggagat agtgttacct cgttttgagg gatcatttgg
     9001 atctcagcga ttgaaaaatc agtgattcct ttttctttta atgcttcttg tatactatgg
     9061 aaatccgcta cttcaccgta aacgatgatt tgtccttctt cttccattac atcgcgaaca
     9121 tcgatgtctt tttccattag gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca
     9181 aagattgctg tattgtcaaa catgtaagaa accgctccgg aaacacccat gttcccgcca
     9241 tttttcccaa atgctgcccg tacgtctgct gctgttcgat tcacattgtt cgtcaatgta
     9301 tcaacaatca ccattgagcc gcttggtcca aatccttcat agcgcaattc agagtatgtt
     9361 tcatctcctg caccttttgc tttttcgatt gctcggtcaa taatatgttt tggtacatta
     9421 taagttttag cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg atcaggatca
     9481 cctgattttg ctgccgcgta gatttcaatc ccaaattttg cataaatccg gctattatta
     9541 gcatctttag cagttttctt tgctacgata tttgcccatt tacgtcccat attttcactt
     9601 cgctttctga tttgatagtt tcataacgca aaaacgtcct gttccattat acttgtgaat
     9661 cacgattctg tgaagctttt tttctttctt ccttcattct tctcattttt agtcaattat
     9721 ctatactcgg attgggattt gcccgaaatt ctgttccgga atattgcctc ccaattgata
     9781 gatttgatca cttctaacta acagttccgc ttcttgtgcc tctttttttc cgattcggga
     9841 ataaatagaa atctcttttt tttcttttaa gaatcttctt ccagcatcgg taaatccaag
     9901 tagtcgaaga taatttttat tttgttggac aggaatctct tgctttttga tattcagcaa
     9961 gacataacaa gataatcgtt ggatccgtgt ccatgtatat ctttttgttt ttacctttga
    10021 cacaaaatcc tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg attctattcc
    10081 ttcggtcatc tgatagatat ctgtcagttc cagcaaagta gaagataaca accgataacg
    10141 caaatacggc caataagatt cccaagtctg cagcgaacaa gttgaaagat cttcttctgt
    10201 ctgaggcggc acaagtccag ctattctttt tccttcttct aacgcatggc gaatggctgt
    10261 tgcactggct atcctctgat ctgtgatgat tttttcatga tagcctgcac ttttacgtcg
    10321 gatcggatgt aattccatag ggtggacata cttggcattt tcctttgcat aacttaatcc
    10381 caagatatga tttggcgaag aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac
    10441 ctcagtcatt ttttgcggat aactatacgt ttgatcagac agttcttgaa atgcctcttc
    10501 tatcctatct tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc
    10561 ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt ttactgcacc
    10621 tttagcaaaa tagtcagctg actgaaccgc ccattcgacg ggaagctcca ctactaaatc
    10681 cacaccattt tgtaaagctt cattcgcgcg atgccattta tcgatgatag ccggttcgcc
    10741 tctttgcaaa aaattcccgc tcattacagc aatgacgacc tctgctcctg ttatttttcg
    10801 tgccatttcc acatgatagc gatgtccatt gtgaaaagga ttgtactcta cgataatccc
    10861 gcagcttttc atgcgcatcc tccttcatag ttatcgataa aaaagaaata gagacaagta
    10921 aaaaaaggca aaaatcggaa aaaatacccg aactatataa actattctcg caaacacaat
    10981 acttctgcga cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg atatataatt
    11041 tatgttgaag atttattctt ttcttgttaa ctcgagcagg caaaaccgct agtctttgta
    11101 tgctacaaag aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa
    11161 tgcttcgact ttcgaaaagc cgctgttttc cagcattctt aaataacttt ccaatggata
    11221 ggtacgctct tgatgtagtt catcttcacg aataaatcga ccagaccctt cttgttcttc
    11281 aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg cttcctggat aactatccca
    11341 caaaaaagca aaattatccc cttgataatg atagctatat tctggaaaaa cctcatctac
    11401 ttgataggtg gagtgtacgt caaataaaaa tcggccattt tcttctaacg cttgatagac
    11461 ttcatcaaac acttgctgta cctcttgtct atttttcata tagcacagcg aatcagaaaa
    11521 acaggtaatt gcctgatatt gtccgatttc tgataaatcg agcatattgc cttccacaaa
    11581 ttggatcgat gcgtcttctt catatgctcg ctggctggca atcatcaaca tctcttctga
    11641 aaggtctaac cctgtcactt tatatccatc ttttgcaaag gcaaccgcta atgctccagt
    11701 accgcatgcc agttccaaaa tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc
    11761 taaccattgc tgataaagtg tttcgtccat cacttcatcg taaatgaaag caaatgtttc
    11821 ataagccata tgttaatcca accattcact aagatcgact agcggagcat ctgaccatag
    11881 tttttccaga ttatagaaat ttcgttctgg cgcatggaaa acatgtacga tcacatctcc
    11941 aagatcgatc aatacccatt ttccgccttc ttttccttca atacgtttta cttcatattt
    12001 gttttcttct tctttatcaa tgatttcttc tgtaattgcg ttgatttgac gttcgctatt
    12061 tgctgaacag atcatgaaat aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat
    12121 aatgtcttct gcccgttttg aatctgctgc ttcaatcgca atttttaatt gttctttact
    12181 atctatgatg atttcctcct aatatgagca gcttattttt ttgctaccca gtaattatat
    12241 gtttcgattg tttttggata aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt
    12301 tttgtttcaa atgctacagc ttcgtctaga tcagcccatg caatggctcg tgcatcttgt
    12361 accccaggaa aatctcgacc tggttcaata tagtctgcta cataaatgat tttgtcaagg
    12421 agactcatct tagcagcccc agtcgtatgc acccggattg catgaagaat ctctgcatcg
    12481 gtgattccta attcccgctc tacaaacgaa gcgccaacaa gaccatgcca gattgcatta
    12541 ttataattca ataactccgg atcaaaaccg tctcttacga ttaccatttt gaactcctca
    12601 tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa
    12661 gcaccgtatt ttttggcaag agccacggct gtttcttcta ctccaagcac atgcttgaag
    12721 cgttgttcgc tcattctcat ctgtattttt tgcatcaggt tttctctaga ataagcagta
    12781 tattcattac tatagtccat attcatacaa cccttcattt tgaatataat caattacttt
    12841 atctggaaca aggtagcgga tagaacagcc ttctttgatc ttttgacgta ttttcgtgga
    12901 actgatgtcg atctcaggta catctaccca aattacagga tatggagtat ccgttgtgta
    12961 tcctggtcga cgtataccca caaagttgac catagaagtc agctcatcga ttttatacca
    13021 ttttggcaaa tattctacca tatccccacc gataataaag taataatctg tatccggatt
    13081 gttttgcgtc aattccttca tagtatcata ggtgtaactt ttcccaccac gagctaattc
    13141 aatcgtttca atctgcaaaa aaggattgtc ttccaccgct aattccaaca tgttgagtcg
    13201 atgttttgca tcaatcgtct gtttctcatc aacatgaggt ggctgataag aaggcatcaa
    13261 aaatacgcgg tcaagaccta aattccggcc agcttgttca gccatcacta aatgtgcaag
    13321 gtgaacggga ttgaatgttc cgcccaagat ccctacttgc tttttcttct ctaagaattg
    13381 aggcatttct tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg tattcatcat
    13441 ggtcactcct tatattgctt ttacttcttt tgatagcctt tgatactttt cttttgtgga
    13501 aggtttgtat aatacaataa cacgtccaat gatttggacg atttcgcaat ctatttcttt
    13561 ttctaatact tgagctgctt cttctgcaat ctcatctgta ttttgaagta acgatacttt
    13621 gatcagttct cttttttcta aagcttcttc aatttgaacg atcattgctg aattgatccc
    13681 accttttccg atttgaaaaa tcggctgaag atgatgcgct ttgctacgta aaaaacgctt
    13741 ttgttttcct cttaaactca taattttcct tcttccttaa atcaatgctt tgcgtttcaa
    13801 tacatctaca ccctttggcg cccagcccgc aattactact ggcgctgtta cagtgatcca
    13861 gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag
    13921 ctccggaaat gcctcgactt catcaggacg aggaggctgt aataatccac ctacatgttt
    13981 ttgataaaat tcgtctgctt tttccagttt cgttcgatga atatttacat catttgacac
    14041 ataagcaata aatgaagcac gattgccgct gataaaatca aatcttgcaa gtccgcccaa
    14101 gaaaagtgtt tgcccttcat tcagctgata aactttaggt ttgatttctt ttaacggtgc
    14161 aatgatccgc aaatcttttt tacctaagta gtgcgccatt tgatggcgat ggataattcc
    14221 cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt
    14281 tcctggaaac tgcgaagttg tgattacatc ttgtactccc gctgtatttt tgatgatttg
    14341 attgataagc gtcgattttc cgacatttgt tactccgacg atatagacat cccgtccatc
    14401 gcggtattct tcgatttttt ctaatagttc cgtcatctca tgagatttct tagcactagt
    14461 taataataca tctactggac gaattcctgc ttcgtaagca cgttccttca tccattgtgt
    14521 taatttagtt tttttcaatg atttaggtaa tatatccact ttgttcccaa ctaaaagtac
    14581 tggattgtca ccgacaaaac ggtgtagtcc tgggattagt gaaccattga agtcaaaaat
    14641 atccaccaca ttgacgatca atgcatcttt ttgccctaat tcattcagta agcgcaaaaa
    14701 gtcgtcatcc gtcaatgaga catcttggat ctcattataa tggcgtaatc tgaaacaacg
    14761 ctgacaatag acttccccgg aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc
    14821 taattcaccg ggtttgtttg tttgtataac ggcaccacag ccgatacatt gtaattcttc
    14881 tgtcattcga ttccacctcg ccagcccata tcagggtgtt ttgctaatag ataggccata
    14941 attctttttt cccaaaaacg attaaattgt gtcttccaac tatctgttgc gataatgggt
    15001 tttacaagga tgctacgtat ccctgcacga ttggctccac gtatatcagt cataatctga
    15061 tcgcctacca ttactatctc atcatctgct aaatccaatt gtttctttgc tatgttgatc
    15121 cctcttgcta aaggtttcat tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct
    15181 cgcgcgacac gttttgaatt attgtttgaa acaacaacaa cagggattcc tgcatttttc
    15241 atttctaaaa tccaagtaag caactcttct gttccatctg gattattcca tgcaatcaac
    15301 gtattgtcta aatcggtcaa tactgctttg atgcctagat tctttaattg ggcaggtgta
    15361 atctgataga tggcttctac catccatgtt ggtttgtaat ttgaaaacat gctgtctcct
    15421 catctgcaaa aaatgaagga gcacataaag tactcctcaa ccccgtatta tactgcatat
    15481 cagcaaaaaa aactacctct atactcgttc gtctttcaga aaaagcattt ctttacaaaa
    15541 tgaccaaaaa taaagattga tcacagaaaa atttagaaac aaaaaacaac taatattggc
    15601 ggaacctctt gtatcgtttt tccagtaatt tatgctgatc taatcgaaca agttccgtca
    15661 atctttcaat caacgctttt ttcataatgt gtgcgacttt atatcgtggc aactgctcgc
    15721 cgttgaacgt ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag
    15781 taattttcat caactcagct gcttctgcgg cgcgactacc gtctttccac aaaatcgacg
    15841 caaatccttc tggagaaagc acagcataaa tcgtttcttc cagcatccag acttcatccg
    15901 ctaaacctag agcaagggca ccgccacttc caccttcacc aatgatgatg gcgatgatcg
    15961 gtacctttaa atcagacatt tcgagcagat ttctggcaat agcctctcct tcgccgcgct
    16021 cttctgctcc tacaccgcaa tatgcacctg ctgtattgat caaggtaatc accggtctat
    16081 tgaacttttc cgcttgtttc atcagtcgta aagcttttcg atagccttca ggatggggag
    16141 aaccaaaatt tctgcggata ttttcctgca gatcccgtcc tttttgaata ccgatcactg
    16201 ttaccggttt gccatctaat cgagcgattc caccaacaat cgcttcgtca tctgcaaata
    16261 aacgatcacc atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg
    16321 tccatcgttc tttagctctt gctaactgaa cgacatcatg tgctgtctta tccacgatct
    16381 atccatcctt tctgtgtatg caattcaaga agcgtatgga ttttctgttt caattccatg
    16441 cggggaacaa tctgatcgac aaaaccatga gataataaga attcagcctt ttggaaatct
    16501 tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag
    16561 ctttggggtt ctgctagaat aatatcgcct tccattgcaa aactggcagt tactcctccg
    16621 gtcgtcgggt ccgttaaaac agttagataa aaaagacctt cattgctatg ccgtttgaca
    16681 gcagcagaga tttttgccat ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca
    16741 cctgaagctg tgaacaagac gactggaaga ctttcctttg ttgctcgttc gaataatctg
    16801 gtgatctttt ctccgacaat cgttcccata cttcccatga taaaattagg atccatcact
    16861 ccaataatga agggaatccc ctgaatggtt gctttaccag tgaggactgc ttcatgaagt
    16921 cctgttttat cttgcatttt tttaattttt tccgtatatt cgggaaatgc tagcggatct
    16981 ttggttacaa gatccgtatc ccattcttca aaactttttt catcaataat caaagctaga
    17041 cgctgccatg caccgattcg aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg
    17101 atgtctttcg tataaaggat atgtttgcaa tttggacact ttgcccacat gttatcagga
    17161 acagatggcg agttagcagg tttgctctct ctattaggat tgatccgaat atatttcttt
    17221 ttcttaaaca gagccatcta tgcgcctcct atctccgttt ctggtgtcca attcggtaaa
    17281 aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg atgactaatc
    17341 aaatccatct gaaattctgc gttcgtgatg attccttctg tgataaattc acttaaggct
    17401 cgctgcatct tcattaatgc ttcaaaacgc gtatttccat ggacgattat ttttgcaatc
    17461 attgaatcat aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt
    17521 cccatcccac ctgctggaag cagtaagttt tggatcttcc ctggagaagg agcaaaatgg
    17581 aaagcaggat tttctgcatt aatccggcat tcgattgcgt gtccttggaa tactacttct
    17641 tcttgacaca cattcaaagg ctcaccggct gctatctcca cctgtttttt tactaaatcg
    17701 attcctgtga ccatttctgt tactggatgt tccacttgga ttcgagtatt catttccatg
    17761 aaataaaaat cgccttcttc atccatcaga aactcgatcg ttccggcatt ttcataattg
    17821 actgctttag ctgctcgaac agcagcgtct cctagtgctt ctctttttgt ttgtgagatc
    17881 actacagaag gagattcttc cagtactttt tgattgttgc gttgtaaaga acaatctcgc
    17941 tcgcctaaat gaatgacatg accaaatcga tcacctaaga tctgaacttc tatatggcga
    18001 gcaggataaa tgattttttc caaatacata tcatcatttc caaatgccgc tgctgcttct
    18061 tgcttagctg aagaaaaatg ctgtgggagc tcttcttttg attgaacttt ccgaatgcct
    18121 ttcccgccac cacctgcagc tgctttcagc atcactggat aaccaattct gtctgctatt
    18181 tccaatgctt cttctactgt agataataca ccagtacttc ctggaatgac tgggacattt
    18241 gcttcttgca tcaattgacg cgcattgatc ttatttccca ttgcatcgat cgttgcactt
    18301 ttaggaccaa taaatgtgat attgcactct tcacacatgg aggcaaatcg gctattttca
    18361 gataaaaaac caaatcctgg atgaattgct tcggcttttg tcacgattgc agcgctcagt
    18421 acttcctgaa cattcagata tgaatcaacg gcttttgccg ggccaatgca gattgcttca
    18481 tctgctaact gtgtatgaag tgcttcggca tctgcttctg aatatatcgc tacggtttgg
    18541 atccctagtt cgcggcatgc acgaatgata cgtacagcga tttcaccgcg gttagctatc
    18601 aatactttag aaaacatatt tcacctatcc aatcataaag gttaattccg cttcggctac
    18661 ttttttaccg tctactttag cgattccttt gcctattccg gctgaagctt tcactttcaa
    18721 tatctcaact tcaaggtata aggtatctcc cggtgtaact ttcttacgga attttgcttt
    18781 gtccaatccg ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac
    18841 tgctcctgct tgagccattg cttcaacaat caatacccct ggcataacag gttcttctgg
    18901 aaaatgtcct tgaaaaaagg gctcattgat cgttacattt ttcttagcaa cgatccgttc
    18961 tccttcaacc atttcttcca ctcgatcaat caaaagcatt ggataacgat gtggaatgat
    19021 ttcttttatt tcttgtatgt tcattgtttt ccctctttcc tgctttgctt atttttgctg
    19081 aatcctaaag agtggctgtc catattcgac aactgactcg ttttctacaa ggatttctgt
    19141 gatcactccg tcggcctcac tagttatctc attcatcagt ttcatcgctt caataatgca
    19201 gataacttct cctttggcaa cttgatcacc tacttttttg aaagctggtt tgtcgggcgc
    19261 tggttgcaga taaacgattc ccacgattgg cgaaacaacc tcgattccat caaatactgc
    19321 cgattctttt tctttcatta tttctggagc agttgttgct tgttcaggta tgacattgac
    19381 tggctccgaa actggcaacg tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt
    19441 atttttattc atatacaatt caaattgacc ttcacgcaaa tcgaattccg ttagcgagga
    19501 ttgatcaaat tgactgacta attctctgat ttcattgata tccaaaatta ttcctcccag
    19561 cgtttcatgc acagaaccgc attatgtccg ccaaacccta atgaattact cattgcataa
    19621 gtaaaacttg caggtttgct ttcgtttttg acaactgtca agtcaatctc aggatcttgt
    19681 ttttctacat tgatcgtagg tgggataaat tgatgttcta atgccagcaa agttgccaag
    19741 gcttcgatcc cgccggctgc tcccaataaa tgtccagtca tcgatttcgt gctgctgacg
    19801 taagtatctc tgaaattttc acctaatgcg atttggattg cttttgcttc tgcctggtca
    19861 ttggctggtg tacttgttcc gtgagcattg atatatccga cttgttccgg ctgtacacca
    19921 gcttcttcca tcgcaagctt cattgcttta gatgcaccag aaccatctgg agtaggagct
    19981 gtcatatggt aagcgtcaca gtttgcaccg tatccaacga tttctccata gattttggca
    20041 ccgcgagctt gtgcatgttc taaagattca agaactagaa cgcctgctcc ttcacccatc
    20101 acaaaaccat tccgattttc gtcaaacgga atcgaaccac gattaggatc agttgattct
    20161 gagagtgctg ttaaagctgc aaatccagca atccccatct cacaaatcgt tgcttctgtt
    20221 cctccagcaa gaatgacatc agaatagcca tgtttgatgt tacggaatgc ttccccaatt
    20281 gaattcgttc cgctagcaca agctgttacg atagctgtac aaataccttt tgcacctaca
    20341 cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca ttggtacgaa cattggagaa
    20401 actcgttttg cacctttttc atgtagacga ataacttggt tttcgattgt tggaagacca
    20461 ccgatcccag atccgatgat gacgccaaac cggtcttgat tcattttttc tttatctaaa
    20521 ccgctcatct ccattgcttc taaagcagcg tagattccat agatcgaaaa catatccatt
    20581 cgtttgctat cttttttaat aaaatattta tcgtaaggga aatctttgac ttcacccgct
    20641 aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc
    20701 tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt gactccatag
    20761 ccagtaatta ccactcgatt catttgtttg ctctcctttt cttatccgtg catgaccaat
    20821 ccgccatcga cattgatgac ttgtccggtg atatatggat tttgtgctaa gaaaactgct
    20881 gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt
    20941 tccttgactt tgtctgctaa aacttccgtc atatcagttg tgatgaatcc tggcgcaatg
    21001 gcattacatg taatgcctct agttgcagct tctcgtgcca cagattttgt taatcctact
    21061 acgccagctt tactagctgc ataatttgct tgaccaatat ttccaatcaa cccagataca
    21121 ctagagagat taatgatggc cccttctcgc tgcttcatca tttttttcaa tacgtgctgc
    21181 gtcatattga aggttccaat caaattgata tccagacatt ttttgaaatc ttctgcatcc
    21241 attcgcatca caagtttgtc atttgtgatt ccggcattgt ttactaacac atggatcgat
    21301 ccaagctttt cttctgcttc tttgatcatt tgtcccgctt tctcataatc agagatgtca
    21361 ccggaaactc ctacgcattt tacaccgaat gcttcgattt cttctatctt ttcttttgga
    21421 atctctccgc gaccattcaa aatgatattt gctccagctt ttgcaaaagc taaggccatc
    21481 gctttgccga ttcctcttgt actacctgtg ataaatacat tttttccttt tacatccata
    21541 acgttcctcc ttatagtatt gctattgttt catcaaatgt ctgcttattc tcaacacgca
    21601 gaactggtat tgttttatcg atttttttca taaatccgct taatactttt ccggggccga
    21661 cttcgatgac ttgttcgatg ccgattgttt ttaacttatg gatactctca tagaaacgaa
    21721 caggttgcat gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc attatttccg
    21781 ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa ttgaatctga cttaggtcct
    21841 tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg ctgacattta
    21901 gagggatcat tcttttgaac cctttttctt ttaatagtaa aacagcttcg tctactgctt
    21961 tttcttcgcc accaatcacg atctgctgag gtgtattata atttgcagga gatacgatgc
    22021 cgtacttgct agcttcatga cagctttcct cgatcgtttc aatcggcgca ttcatcactg
    22081 caaccatttt cccgcttcca gctggggcag cttctgtcat ataagcgcct ctttttgcta
    22141 ctaacgcaac tgcttcagtg aaggatagcg cattactagc tactaatgcc gaatattctc
    22201 ctaaactcag tcctaaagct gcatctggga tgattccatg ctccttcaat aagcgataat
    22261 acgcgatgct gactgttaga atagctggct gtgtatattg cgtctgatcc aaacgttcat
    22321 tttctgtaaa gcaaagttca gccatatcgt agccgagaat ctcacttgct tcatcaaatg
    22381 tctgctttac aacggcttct tcatataaat cttttcccat accttgatat tgtgcacctt
    22441 ggccactaaa taagaatgct gttttcatgt ttttctccaa tatatccaga atatatgttt
    22501 gattcttcct attcaaaaag gccgagacat tcctatttca ggaaatgtcc cagcccccaa
    22561 cctaatttta atgattatgc agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt
    22621 ttgattccgt cttctgtttc gattttcacg tcaaattcat cttcaatttc attgatgatt
    22681 tggaataaat ccaagctgtc agcttctaag tcttcttgga tgtcagtagt caatttgatt
    22741 tcttcagttt cttttcctaa ttcttctgcg atgatttctt gaattttttc gaataccata
    22801 ataatatatt cctccgatat tgtttgcttt ttatttttat aatttaacag tctacaactc
    22861 catgagaata cttccccatg ttaaacctcc gccatagcct gtaaatatta ctttctgctg
    22921 ttttcctaag gtgatctttc cactagaaac tgcttcatcc aataagatag ggatgcttgc
    22981 agcagatgta ttcccatatt tatccatatt ggtcaggaac ttttcttgcg gcatctttac
    23041 ttttcgggca attttctcaa taattctgag gtttgcctga tgcaaaagca gataatcaac
    23101 tggtttgtct ttcatgactt cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa
    23161 aatatctctg ccagtcattt gcaaataata cgaaccttct gaatcttctg aatagaacgg
    23221 atttttattt tgctggtagc cagcggtcaa tgagctactt ctggtaccgt cagctgctaa
    23281 tttttctttc agcaattttt tctgtggatt cgcttctaag agaatcccac ctgctccatc
    23341 gccaaacaac acagcagtcg tacgatcaga ccaatcgatt agcttagata gtgtttcacc
    23401 accaatcacc agtcccttag ttgagccgca gcggatcatt ttttcagcca tgcttaacgc
    23461 ataaacaaat cctgagcacg cagcactgat atcaaaagcg atagcatgag ttgctcccaa
    23521 tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac
    23581 taaaataaaa tccaaatctt tagggtttag atttttctgt tttatcaatt tttccgccac
    23641 taaaatgcat aaatcagaag tctcctgatt tgttacacat cttcgttcac gaatacccgt
    23701 ccttgacgaa atccattcat cagaggtatc catcatcttg gaaagatcat tattagtaat
    23761 aatcttttca ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt ccattaaata
    23821 tcctcactta tagtcttgca agaagtcgtg caagtttttc agtgcatcca gcaaggcatg
    23881 ctgttcctcc gtagccattc cgtctaagat gttcttgacc atttcacgat gaaaatgctg
    23941 atggacccga aatagtaact ttcctttttt cgttagtccc aactttacaa cgcggcggtc
    24001 atcttcactg cggatacgtt cgacatagcc tttttttact aacttgttaa tagcagtggt
    24061 caaggtccca acagtaattg atagttcttt ggctacttct gaagttgttt ttttcttgta
    24121 catcccgatt gcctcgatcg tgtgcatctc tgtgattgac agatctttga attgagattt
    24181 tttcagctca gactcttcga ttgtcaaaat atcattgaag acactaacaa gataatcatt
    24241 tactgttttt aaatctggtt
//
LOCUS       DAZUMV010000031        23728 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000031 DAZUMV010000000
VERSION     DAZUMV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.031"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(730..1343)
                     /locus_tag="ACG8FK_001112"
                     /pseudo
     CDS             complement(730..1343)
                     /locus_tag="ACG8FK_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2564..3268)
                     /locus_tag="ACG8FK_001113"
     CDS             complement(2564..3268)
                     /locus_tag="ACG8FK_001113"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5096737.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
     gene            complement(3283..4152)
                     /locus_tag="ACG8FK_001114"
     CDS             complement(3283..4152)
                     /locus_tag="ACG8FK_001114"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS5096738.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            complement(4260..5276)
                     /locus_tag="ACG8FK_001115"
     CDS             complement(4260..5276)
                     /locus_tag="ACG8FK_001115"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5096739.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            5466..8189
                     /locus_tag="ACG8FK_001116"
     CDS             5466..8189
                     /locus_tag="ACG8FK_001116"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5096740.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            8317..8739
                     /locus_tag="ACG8FK_001117"
     CDS             8317..8739
                     /locus_tag="ACG8FK_001117"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5096741.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            8757..9227
                     /locus_tag="ACG8FK_001118"
     CDS             8757..9227
                     /locus_tag="ACG8FK_001118"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5096742.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            9251..10033
                     /locus_tag="ACG8FK_001119"
     CDS             9251..10033
                     /locus_tag="ACG8FK_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5096743.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            10023..10847
                     /locus_tag="ACG8FK_001120"
     CDS             10023..10847
                     /locus_tag="ACG8FK_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5096744.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            10882..11880
                     /locus_tag="ACG8FK_001121"
     CDS             10882..11880
                     /locus_tag="ACG8FK_001121"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5096745.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            11897..12751
                     /locus_tag="ACG8FK_001122"
     CDS             11897..12751
                     /locus_tag="ACG8FK_001122"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS5096746.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(13325..14575)
                     /locus_tag="ACG8FK_001123"
                     /pseudo
     CDS             complement(13325..14575)
                     /locus_tag="ACG8FK_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            15092..15283
                     /locus_tag="ACG8FK_001124"
     CDS             15092..15283
                     /locus_tag="ACG8FK_001124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096747.1"
                     /translation="MGKYSFEFKSTIVQEYLEGEGGLGYLAKIQGVRSKKGTRMDQSK
                     VRPKNLTFGGRYLYGKISF"
     gene            15264..16675
                     /locus_tag="ACG8FK_001125"
                     /pseudo
     CDS             15264..16675
                     /locus_tag="ACG8FK_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            16750..18033
                     /locus_tag="ACG8FK_001126"
                     /pseudo
     CDS             16750..18033
                     /locus_tag="ACG8FK_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(18249..19061)
                     /locus_tag="ACG8FK_001127"
     CDS             complement(18249..19061)
                     /locus_tag="ACG8FK_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5096748.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            complement(19078..19809)
                     /locus_tag="ACG8FK_001128"
     CDS             complement(19078..19809)
                     /locus_tag="ACG8FK_001128"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5096749.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            complement(19826..20281)
                     /locus_tag="ACG8FK_001129"
     CDS             complement(19826..20281)
                     /locus_tag="ACG8FK_001129"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5096750.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            complement(20274..20684)
                     /locus_tag="ACG8FK_001130"
     CDS             complement(20274..20684)
                     /locus_tag="ACG8FK_001130"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5096751.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            complement(21215..22174)
                     /locus_tag="ACG8FK_001131"
                     /pseudo
     CDS             complement(21215..22174)
                     /locus_tag="ACG8FK_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(23035..23250)
                     /locus_tag="ACG8FK_001132"
     CDS             complement(23035..23250)
                     /locus_tag="ACG8FK_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS5096752.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
BASE COUNT         7764 a         3857 c         4484 g         7623 t
ORIGIN      
        1 ttgtacgatt caaagatagt agtaaagtgg aagtgtagaa aatagggata gatgtctttt
       61 atttgttcac attttataac gaagtattta agacagctat tgaataatgt ttcctccacc
      121 tttttcattc atttacccca ccccgttgtt atatgaccgt gtttcgagag aaaccaataa
      181 aaagcatagg ctaactaggg gttccttttt cttttgattt tttggaatcc gcttaagtga
      241 aatgtatctg aaatgaaaca gagataaagt gattttttgc tgtaatgaca aggttttagc
      301 tgtttcagat accatgtaac ccaaaatagt ttattgagaa aataggagag tatttgatac
      361 taactagtag gaaaaaatga gataaaaagt aaatggaatc atttcaggta atctcaaata
      421 aaaaatatac aggcctgtgg cactttacct acgggactgt atatttttta ctctcagcgg
      481 gcttgttttg actccgtgtt tgactccgtc gtattgattt taaatgatat tcgatgacca
      541 attaataggt cgtttttgaa atttttgtct ctatatgact cagtttgata attattgata
      601 gatcgatttg ggtctatatc gaacaaggga agattgcaaa agtcgaagct taaaggattg
      661 atattaaagg attaagaggt aaaaaggtat ccccataata tcctctgact ccgtttttga
      721 cctcgcttat catccgttca tgtaagaatc aaataattcg actgtttcat tttgccgatt
      781 ttttgatact ttaatataaa tgtccatgat tgttttagaa gaagaatgtc ctagcctatc
      841 ttgtacatct tttacttgag cacctgcttc aaagagaagg gtagcatgag tctttctgaa
      901 gccatggact ttgattatct tcatatctgg gaacttccgt gctactttat ccatgacatg
      961 gttagggtag cctttgtaaa ggaagtccct ttttttattc cctttgatag tgctgattac
     1021 aggtatatca tctatattat ctatccgaat ccctctagaa aggttctttt atgttacaga
     1081 acgtttccat tttttagaat acgtaaggtt ccattatcaa ttgaaattac tccctttgat
     1141 ttgtctgttt ttgtaggatt aatacataga gagccctcgg atagttctac taacgttttg
     1201 ttgaagcaga tagtttgatt atcgaaatct atgtctctac atgtcagtcc gagtatttct
     1261 ccttttcgtt cccctgtata agaagctatt ctaaagaata ctaaagcacg ctcatctttt
     1321 aacttatgca aacattcaaa aaacagatat ttcacatata tctaaaattc ggagattgaa
     1381 agaatgaatt tatctaagaa ttttcactaa tgttgcgaat ataatatcag aaaatttcga
     1441 aataaatttt gttaaaaatt agaaaaatcc gaatacttag tagtggataa atttttttca
     1501 ggaatagtac gttttattaa aatgaaaact aatccgacat caactttttt atcgaagcaa
     1561 acatcaagcg gtaaaaagac ctctttagtt tgaaaagtgt tgctttcata ttgatttaaa
     1621 ttttggttaa gtaggtaaat taagaaatga tcagtgtaag tatttcctga tttagcgcac
     1681 cttgttcact ttgaaaatac aggtttttga tggacagatt ttgcttcaaa tgtttaaaga
     1741 acatgccact gtacttggta caggtcggcg cccccatttg gagttgcgtc agatcgagtc
     1801 gtgacgattc attttgtaca aattagaatt taaaattgaa tacatgacgc gcacattttc
     1861 ccagagagcc atattctgta attaatcaca gcaattagtg attatgcaca atctaaaaaa
     1921 ctgatttata ataaaaatat tattcaaata aataataata actgcccgca tgatgttgac
     1981 agttacaata gcacatagat agactggcaa agatggttct taatctttca ctttggtcag
     2041 aattcttggg ttatccttcc agtgaaagtc agcagaaaat ccttaatgct tacaaataat
     2101 ttttgcgaaa taggttttat tggtaaagga ctcaatcatg aatatgaatt acttttatct
     2161 ggtaaatttg gaaaaatttt gcaaagaaat aaaaattaga caaatttgct acacataatc
     2221 aaagattaaa aaagttttta aaatatagtt gtttgaatgg ggatatgaat tagagaaaat
     2281 tggttgattc aacgggtgct atcatttttg atagcgtcat atgtgaagta agcacaccta
     2341 atgaaaaagg ttcaggcttg ctttgaagac ttattaacaa ctatgtcgac tatccttgac
     2401 ttagggtaga ggagtacctc cacacacctt aattagatga taatgtttgg actgtaggct
     2461 acttagcgga ttaagataaa gaggaagtag aaaaagagcc ttgcggaaag acactttatc
     2521 tgttatccgc gaggctcttt atacatttaa gcatatttaa aaattatttg gaaacagtac
     2581 tttcaaactt tgtcttataa gcagggtact tagatatttc atattcaaac ggtttctgat
     2641 ttttagcaaa tccaatactt gtaaagacaa agactggctc atttttttct aaatcaagat
     2701 aaaggttttc taaatctgtt ggcggtgtaa cttcaattga gcgtttggca taagctaatc
     2761 taattcccct actttctaat actgagaaca gggattcttt ggcaaaatca tgttcaacaa
     2821 tttcaggtaa aacagatagg ggaatgcaac tttttgcaat acaagttgga tagtcatcga
     2881 tgtatcgaat tctttccaag acaagcacta aagtattctg ctcaatctcc aaacattcac
     2941 taattgttga atcagcaggt atgatggttt gttggataat cttagaactg attttctttc
     3001 cttgctgaac cgcatcttgg aagaatccga taaaagagtc gagttcgtgg tcaacatatt
     3061 tttctgaagc tggtttcact agatacccgc gaccttcatt tttagaaata attccttcgt
     3121 cttctaaaga agctaaagct ttccgaattg ttggacgact caccgaaaat tcctgggcca
     3181 aatctctttc cgaaggcaat aattcagatt tccgatcagt ggtgatatac acttttcgaa
     3241 tttgttcttt tactttgtca aaaatcatag taatccatcc ttttaccaag gtattaacat
     3301 tatattacca tttttttgac ttaatatccc agattttcct tcaattaatt ccttcgccgc
     3361 taattgaccc cacaaatagc caatatatcg gtcataaaaa gttggagatg atccacgctg
     3421 aagatacgca agatttaagt atttggtttc ttttggtaaa tttttggtaa taaattcaat
     3481 gattttttgt ttgttagata taccttctgg taaaagcaat ataggcgagt agaactccga
     3541 ctcaaatttt ttacatatta agtgtaagta ttccttgtcg tctctctcaa cccaataagt
     3601 attatttgca atggaagcgt actctggaag agcaaagcag cctcggccca tgacttccat
     3661 tataataata cgatcatgag cattagcagt agaagttaaa tcatcgattg atcggattat
     3721 agtgtttaag gcgctatcga agccaagtgt ataatcggta ttggggaaat cgttatcaat
     3781 agtccctggg atgacagata cttgtaattc tgattcattt ttcaatagtt cagctccttt
     3841 caaggaaccg ttacctccaa taacaattaa gttgtcgatc tccatcttat ttaggttttc
     3901 aataataatt ttccgacttt cgagatctct cattttctca aatctggctg ttccaataag
     3961 tgttccagct ttgaatacct caacttcttt tagagaaact gaggcaatac attccttaaa
     4021 tattccggca taaccattct taaaaaagac tacttccgaa tctggttcgc tatcaattgt
     4081 tttttttatt gctaataaag ctgaattcat tccgggagag tcgccaccac tacagataac
     4141 tgcgattttc attttagatc ctcctaaaaa aggtgactgc gattctattc tcccattaga
     4201 gaaaatgaag caatcaaatg ggcaatggag tcgcggtcac ctttttcata tttttatgat
     4261 tagtattcca tttgcttata ataacggcga gtagttaaag ggtggttacg ttcttcttct
     4321 agaaaagcac taattcgttc aaagacagta gctaagacga ctggggaaat aatttttcgc
     4381 gtgtcttccg aaatgccagg taagtcatag tcctttgtat caaccgttac gtgaacatct
     4441 gtaaacttag gaacgaaatt cttcacgcgt tctgtgagag gtcgagaatc atcttctcct
     4501 tctgtaatga tgacacttac acccgcttca attaattcaa gagttccatg gaagaagtct
     4561 gctgcatgaa caggacgagt cttaatccat tgcatctctt ctaaaataca catgccatag
     4621 taatatgctt ccgcaaagct atcaccagca ccggttaaaa tatgatatga agtatctttg
     4681 tgtttttttg caaaagattt tgccattggt tccgcttgct ctttagcgtg cactaaggct
     4741 tcaggtattt tttttaaatc ctcaatatag gtattgtatt gattgatatc gatttcattt
     4801 ctttgtttca ttaagaagag agcaatcagc agtccttgaa ggtaataatt ttctgaagat
     4861 gtatcatcta aaacgtcgtt atagatagaa tatgttgcag ctttttctag gggcgtttta
     4921 ggacttccta ccaaagctaa tgttttgacg ttcatttcat tacagtattc gactaactca
     4981 agtgtttctt ttgttgtacc agataaagat gggataacaa ccaaagattt gttagaaaat
     5041 gttttattgg gatgaatcat aaattcggca gcgataacac gatgaaaagg gaaagtgcta
     5101 cgagttctcg cgaattcgat tgttgaatca aaaagaatac caactcctcc agacccaata
     5161 aagtaaattt cttctaagtc agatgcgatg gattctaaaa tattggaaat ttgttcattt
     5221 gttgcaacgg caccagcttg agtctttaaa aatctttctt gatcaaaatt aaacataaaa
     5281 atctctcctt tgattaccaa tggtaatatt ttgtttgcat aaataaaata ccacaggtaa
     5341 taaaaagtgt caaatatttt ttaggaaaaa taccataggt attgttaaag tggaaagatg
     5401 tttataaaga atattgaatt ttatagtgaa aatcatttat tattaagtaa aggaggtggg
     5461 ttcatttgaa aacgaaaatt ttaaaagaat taaatgatct acttaacagc tatgattttc
     5521 cacaatattt tccagaagaa tgtaacgcgc aatatattgc ccaaaaattt caaataaaaa
     5581 gaaatacggc tagtaagcat ctaaacgaac taatggaaca gaaaaaagtt gtgaaaatca
     5641 atggtcgtcc tgttcttttt tttccagtcc atacaattga acggtatata tcacattcat
     5701 tagatagttt tgagttttct tcaattgatt cgtttagaga aaaaatttca aagaaaaaag
     5761 cagatcattt ttttgatgat ttaattggtg caaaacaaag tttaaaagaa gcggttcaac
     5821 aagggatatc agcaatgaaa tatccaggtg gattaccaat aatgtatttt ggatcgtccg
     5881 gagttgggaa gagttactta gtggaaaaac tagctgagta ttgccgacaa actggtatta
     5941 ttcatgagaa tgctccgttc atcgagttaa actgcgctca gtattttaat aatccagagt
     6001 tattaacaag tcagttgttt ggccatatga agggatcgtt taccggcgcc gattctgacc
     6061 atattggtat aatagaagca gcagacggtg gaatcgtctt tcttgacgaa attcatcgtt
     6121 taccaccaga aggacaagag aaacttttta ttcatatgga taaaggcgtt tttcgaagag
     6181 ttggagatag tggtccttgg cgtttatcac atgttagata tatttttgcg acaacggaac
     6241 aggaagaaaa tttttttcta gaaacttttc gacgaagaat tccgattgtt tgtactttgc
     6301 ctaattacca agatagagaa attagcgaac gaaaagagat tgtcttccat ttattagaga
     6361 aagagtctac tctgatcaat aaagaaatat tagtttctga agccgccctt agtttcctga
     6421 ttcgcttaga tttgtatgga aacattggtg agctggatgg actaataaaa caaactattg
     6481 cgaataaact atttagtgga agcaataaaa aatatatcga aattcatatt tctgatttac
     6541 ctagcaggta cttgctaaca gaaaatattg agttaaaaaa ccaaattgcg agccaacggt
     6601 tctatatctt tagtaatggt aactatgttc atgattgtga cgagacttca actgtaacta
     6661 caaatttacg aggtttgatt catcgtgttt ctaaatatta ttcagagtgt catgatgtga
     6721 atagaaatag tgaagagttt cgacgttttg tgaaggacca aatggcagaa tttaatgatc
     6781 gttttattta ccaaaaaaat tcagaagtct tagttaagta ttatttaccg gaaatccaaa
     6841 atattgttca aaaaatctct cccagtcttt ttgttcacct ttctggcaat acggtccaag
     6901 ctatagctac ctatctcgct attagggatc aatgggggta tgggaaagag aatgcttcag
     6961 cgaatgaaat tgaaaagtta tatgaaaacc taaagtgtaa ctatttgacg gatttaaatg
     7021 tattcttact gatcttcgag aacgccctgg atacaacatt gactatttgg gataaactgt
     7081 atatcagttt gtgtgttatt agtgagaccg gaaatgaagc gaaagacatt aacgcaatta
     7141 ttttagccca tggatatgcg acagctagta gtattgctaa tgttgccaat cgtttggtta
     7201 atcagtttgt catagattct attgatatgc ctctagatat cagctttagt gaagtaattg
     7261 acagattact tcactatata gagtatcgaa agcctaaaga tggactagtg attttcgttg
     7321 atatgggaag tttagctcaa attaagactg aaattgaaca agtaatcgaa gttccaacaa
     7381 tgattattaa taatgttaca accgaaatgg ctattgaaac cgcacaactg attcaaagta
     7441 cgagtgatat ccaaaaggtt gtaaaaaaat tgccgtattc tcagtttgaa aagcaagttc
     7501 tatatcccat caaaattaga aaacgtacaa tcgttgtttc ctgcaatact ggtttgggta
     7561 cctccataaa aattaaagaa atgatggaaa ataatttgtc gaaagacttg ggcattgaat
     7621 ttctgccata cgaaaatgaa actcttagag atactcagca attagagttt ctcattaaaa
     7681 caaaggatat cattgccatt atcggaacag atgatccgcg tgtgtcgaac attccctata
     7741 tttctcttga agaattattt gatggtgaaa ttggcaaact ctataaagtt cttttgagag
     7801 tctatggaaa agagattgct gaggcaatta atcaaaatat tgttaaaaat agctcattaa
     7861 accgcttgat tgacagtctt actattttag atgccgaaaa agttgtcaat caaattgagg
     7921 aaagtttaaa agattatcaa atgagggtgg ggaaaacgtt atcatcattg tccaaaatta
     7981 atctttatgt tcatatcagc tgtatgattg agcgactaat tcgtaatcag ggagtaagta
     8041 gttatccagc aatagatcag tttcttaagc gatacgaaac tgaggttgct tctattcaaa
     8101 aggcacttag taagattgag aaactttata atattaagat tcctatggaa gagattgcat
     8161 atgtgtgcaa catcattgta agttattgat aaaataaagt gacactattc acagcgatgg
     8221 aaatagtgtc actttatttt ttgtacgccc tttcatttaa gtggtgacag taattggcac
     8281 agtatttgct aataacaaag cgaaaggagt tcgggtatga gacaaatatt atgtatgagc
     8341 cacggtcctc tagcaaaagg aatgattgaa actttgagta ttatcgtagg agatttggat
     8401 aatattgatt ttcgatgtgc ctatgtcgat ggaaataatg acgtagaaat gatcatcgat
     8461 gattatctaa aggaatctat gggaaatgag acgattgttg taaccgacat ttttggtgga
     8521 agcattaata atgagtggct aagacggttg ccagatcttt ctaaagtcta tttgatttct
     8581 ggaatgaatt taagtctttt agtggattta tatttaaaat tccaattagt ttcatctgat
     8641 cagattgaag aaattattga tcatgcaatt caagcatctt ctggttcgat taagttttgt
     8701 aatcagctag attatgagca aacggttgat gacttttaaa taaaggaggg aagagaatga
     8761 ttaaattagt gaggatcgat catcgtttga tacacggtca agttgcttta acttggacga
     8821 acgcaatcag tgcggattgc attttagtag cgaacgatga aattttgaat gatccaattc
     8881 aaaagacaac tttgaaatta gccgcaccac ataacgtgaa ggttgttatc aaatcaattc
     8941 aagattcgat tgatgcaatt aatagtggga agacagataa atacaaacta tttgtagtta
     9001 ccaaaaccat ttccgatgct aaacggttgg caaatggggt tgaacaaatt aaatctgtaa
     9061 atatcggtag tgttaaggaa actagtggca gtagcgcttt gacgcataca atttttgtaa
     9121 cagaagagga acgagcagaa cttaaaggaa tggtctcaga gggcattgaa gtgttcgctc
     9181 aacaagttcc aacagataag gcaatagatt taaaaaaatc tttgtaataa aaaaataagg
     9241 gggtaatttc atggatagtc tcttagtaca agcaattttg cttggtctct tgactgcgtt
     9301 catcgtaatt gactggatga caggtacgaa taacttatca agaccaatcg tatcttccgt
     9361 ctttgttggt ttaattatgg gcgatcttaa gatggggatt atgatggggg caacactgga
     9421 gttagcattt atcggagcaa taacaatcgg agcggcacgt ccgcctgata cggtatctgg
     9481 tgggatttta gggactgcgt ttgctattgc aactggcaag ggagcagaat ttgcgttgac
     9541 cttggcattc ccaatcgctg cgctattctt attagttgat aatctactaa cattgcttgt
     9601 tctaccaatg attgctagaa aggcagatag atatgctgaa aagggcgaac taaaaaatgt
     9661 gtcgaaaatg catgttttag gttttatctt agtaaaaagt ttaccacggg ctttatttgt
     9721 cggattctca ttttacttag gaagtcctgt tatggaacga atcttaaatg ctattccaac
     9781 ttttgtccaa gatgggatta acattgctgc aggcattatg ccagcactag gttttgctat
     9841 tttattgcaa atgcttctta agaaagatgt ctttgtatat ttcgtaattg gttttgcact
     9901 ctttagctac ttacatgtgc cgattctggg gattgcaatc ttcggcgcat gtctagctta
     9961 tattatagta aacatggaaa ccaaagctgc aatgggttca agaggggagg agatggttga
    10021 agatgacttc tagtgggaaa gctttaacta aagatgattt aacaaaaatt tgtattcggt
    10081 catgtgcatt agaagcatca tggaattttg agcgccaaca acatatgggc tttgcttttg
    10141 ctatgatgcc agcgttagaa aaaatttata gtggcaatga agaagaaaag caaaaagcag
    10201 ctcaaagaca tctagagttt tttaacacag ctccgccatt atcaacattc ataatgggaa
    10261 ttgtggcttc tatggaagaa gaaaatgcca aaaatcctga tgactttgat acttctacaa
    10321 tcacttctat taaaactgca ttgatgggac cattagctgg tattggtgat tcaatgtttt
    10381 ggggtacctt attagttatc ggtatcggtg taggtgcttc attagcaaaa gaaggcaata
    10441 ttttgggtcc atttttattt ttactaatct tcaatgtccc taattttctt ctccgttact
    10501 ttggagttat gcagggatat aactttggta caaagctatt agagaacatt gaatcctctg
    10561 gcgtcatgaa aacgttgact catgctgcct caattttggg tcttatggta attgggggca
    10621 tgtctggaag tatggtatca atcaaagtag ctggttctat tggacaaggt gatgaagcaa
    10681 caaaaattca aagtatcatt gatggtattt taccaaatct gctgtcagta ttatgtgtat
    10741 ttctgattta ctggatgctt aaaaaaggct ttaaaacaac ttacgtgtta ttcggaatct
    10801 ttatctttgg tatcatagca gccttcttcg gatttttgac aacttaattg aataatctaa
    10861 ataattgtaa ggagagaact aatgttaaac tataatgcaa aagaaatgaa aaaaaaaggc
    10921 aaacggattt acggtattcg tgaagatgta gaaaaaattg caaagcaagc ttatgattct
    10981 ggaattagta atattttctt tactgcttct ggtggatcgg tagccgtaat gcaaccattt
    11041 gagtatgtat tgatgactga atcaagtatt cctgtttact ctatgactgc agctgagctt
    11101 gtcttaatgg gacataaaca gttgaatgaa aaatctttag taatcttata ctcaaaatct
    11161 ggagatacga aagaaactat taaggcagct gaatatttaa aagaacgaaa cgtgccaacc
    11221 gttggtattg taagtaaaga agatacaccg ttagaggctc tatcaactta tgcaatcaat
    11281 tacctagacg gccgccctca agaaatgcca ctttatttct taattatgtc tatcatgaat
    11341 ttgaatgggg aatttcctga atacactcgt tttgctgatg aactgaaaaa cttaccaacg
    11401 gctctgaatt cagttagaga acaggctgat gaaaaaggga agaagtatgc cgaagaattt
    11461 aaagaagatc cttatcaaat ttggattggt agcgggaatt tatggggaat cacctactct
    11521 tattcaatgt gtgtgcttga ggaatctcaa tggttgcgta caaagtctgt ctcatcacca
    11581 gagtttttcc atggtaccct cgagttagtc gaaaaaggag tttgtgtaac acttttgaaa
    11641 actgaggggg aaacacggct tttagatgct cgagttgaaa aatttgcggt ccaatataca
    11701 gataaattta atgtttttga tactaaggat tatgagctac caggtattag tgacgaattt
    11761 cgaaagtact tttgtgctcc agttatgtgg gcagccctag gtcgcgtcag tgttcatctt
    11821 gaagatattc ggaaccactc tttggataaa cgtcgttatt accgagtagt tgaatactaa
    11881 aagtaggtga ttaattatga cagcgtatgt gattggattt ggcgataatg ttgttgatta
    11941 ctatgagaat ttgaatcaaa aatttccagg gggaaatgca gttaattttg ctgtgaatgc
    12001 aaaaaagagt ggtgttaaag catactatgt cggtagtcta tcatctgatc ctgatggcgt
    12061 gttattacaa cacgccatca gttctgaagg tgttgatatc tctttctcgg aagttattga
    12121 cgagcctact gagcaagcac atgtaactat taaagatgga gatagggagt ttataggtgg
    12181 cgtgcgtggc tcccgattga cacctgctct ctcagcagaa cttattgatt tgatgaagaa
    12241 cagttcatta gttcatacta gttgccatag tcgaacagaa acaaaaatta aagaattaaa
    12301 aaaatatgat gttaaagttt cttttgattt ttctgactta gataaatata gaacgcatga
    12361 atacttgtca gatgtttgtc cgtcaattta tattgcacag ttttcagtcg ctacagatga
    12421 tcaagaggaa attaatcgct taattagtga atgcaaggac tttgaggttc cgtatcttct
    12481 ttttacgcgt ggtagtaaga gccctgtttt tgttgacact cgagagaata agacttatga
    12541 aggattcatt cgcaaagttg agtcacctca tgatacaatg ggcgcaggtg atgcatactt
    12601 tgcagctttt gcaagtaatt ttgtcttatc taaacgtgaa aaatcaatga tagaacatgt
    12661 tattgattgt tttcacaaag gtgcaaacct tgcatatgaa acattgatga ttgatggctc
    12721 ctttggtcat gctacaccta ttaaccctta gctttgtgct ttgggctttt attgctggaa
    12781 tttgtatagt agactcatca taactatatt tttgtatgtg aaaaggcaag aggctatgat
    12841 gaaaataaca ctggctaatt gcatgaggga gcatatcgtt gttaaagcgt cattgatagt
    12901 atgaataaat atctcgataa tattatattg ttatttactt aatttattga ttagtcaagc
    12961 aaagataatc ttctgttact taacgagact agtaggtaca agcacttcat gttatgtacg
    13021 acccaaaaat attgctgttg attatttgct gaaattattg cgagcttcaa tttattttta
    13081 aaggacttgg taggctgcgt gatgaaataa gacttggaag gctgtctaaa ccaatatata
    13141 gtagactaaa tagtaaaaaa gagcatggta attgtctctt tgtcaataag tctaatggta
    13201 ataagacaag ttggagattg agaatatttt tattgtgtaa cctattttga agcgtactta
    13261 actagccccg gccaaaatca aattttttcg gggtatatca agggtttgcg tcaataacag
    13321 aatgttattt atctatcacg ttccttggtg gcgtgtagag ttgatggtta cgtagtagcg
    13381 tatctaccag acgcgtaaat tttcttgcgg ttaagacgtg tgctcgtttg tgtttatttt
    13441 tgggacctct ttacatttgc tttgataaaa agctttatat tgaggaagat atttttttac
    13501 agagttggaa gcttcaacta agtagtatct aaaatagcga ctcctttgtt ttgtcagagg
    13561 tgtattttgt gaccgatagt taacagattg tttgcctttc caacataagc ctgcgtattt
    13621 tgccaatttt gtttgatctt caaatcgttc aatttgaccg atttcagcga ttaatccagc
    13681 ggcgtgtacc tttccaatac ctggaacact agtcaaatat tgatattctg gaataacgac
    13741 aacaaattgc tcggttgctt tatccaagtc tttgatgttc ttttcaagcg cacgaatttc
    13801 acatacaaaa acatttagaa cgacattgat ggattcttat gcaagactat ctaaccgata
    13861 actcacggca attgcacgtt gaatcgattt ggcatttttt tctggatctt taaagcgaca
    13921 tttccttttt cttttagtaa gtcacagaaa gcttccaaag gaagcacagc caattcatct
    13981 agcgtgaagt cttctgtcac aagagtaatg acagtggcac tatagagact tgtcgattca
    14041 tcgcgcattt atcgagtaag cgtattgcat ttgtacgtta gattttccag aaagtgttgt
    14101 tttgtacgag tgaagttgct taatgagtcg atagcgagtg cgtgttaaac gctgtaaagc
    14161 cacgtacctc tcttctttga taggagaagt tgtgaaccgt tgaatacgca ataaatcagc
    14221 aattcttaac gcgttgtttc gatcagtcgt atcttcctca aacatgcgac tgaattgttt
    14281 catccgaaaa ggattttcaa tcctcacaag tgcattgagt ttttttagct ctgcatcttc
    14341 atggaaaaac atcgaaggat gaaagctgta catggacgtt taattccatc tcaataacga
    14401 tttgaccgaa gtgattgttt tcattgaaca caagaatcag ttctcgtgtc aatggagccc
    14461 tttcaatgtc attagcaaca gaattttcag tcaatataga tagctgatcg tcgtctgtta
    14521 ggaagcaaac gtctaatttt tcagaactaa cgtcattacc aacaaataat ttcatggaat
    14581 aaaaacctat tttcgatata aattctagaa aatgcttcga tactgtggga ttccacagaa
    14641 actcactgtg tggtcacgac ctcgcctaat agaatacggt aataaaagag ctaatgagtt
    14701 gatagtaaac acagcgcttt ggataacaga cttatgaagg tagacaaagc tacaggactg
    14761 attagcaaat tctcgttgaa tcctttccat acatccattg tactctgaga aatcacacaa
    14821 taacgaaata aattccggaa ggaatatgtt attcaaaaaa atttgtgttt tatagatact
    14881 ttaaaagaaa cttattaggc aaaatatatt ttacgaagac tgatgagttg tgcaaaatca
    14941 aatctgagtc agaatgaact tcattctagc tcagattttt gtttaatgac ttcagtcagt
    15001 ttcttgcatt acgtgaatga aaatgggact ggtgatgtcc aaaccctaaa aagttagacc
    15061 aaaatctaac ttcagggggg atggtatttt tatgggaaaa tatagctttg aattcaagtc
    15121 aacaatcgtt caagagtatc ttgaaggaga aggtggatta ggatatttag caaaaataca
    15181 aggagtgaga tcaaaaaaag gtacacggat ggatcaatca aaagttagac caaaaaatct
    15241 aacttttggg ggtcgttatt tgtatggcaa aatatccttt tgaattaaaa ctaaaaatcg
    15301 ttaaagaata tcttagtggc gtgggcgaat atcggcatct tgccaacaaa tacggtattt
    15361 caagcaagtc tcaagtaagt gattgggtga atgcctatcg tgaatacgga gaggaaggtc
    15421 tattgaaaaa gcatcaaaat cagaaatatt ctgttcaatt taagttaaat gcgctagagt
    15481 tatatcaaac aagtgagatg tcctatcgag aagttgccag cactttagaa atgaacaacc
    15541 ctgcgcttat tgccaattgg atgcgtaatt tcagagaagc aggtattgag gggctctcaa
    15601 aagagaaagg gcgaccactt actttgacta gaaaaaaaga aaacaaaaaa gaaaatacaa
    15661 caactgaaga acgcgagcgt atcaaggcat tagaaaagca agtccgatcc cttcaaattg
    15721 agaatgcctt tttaaaagag ttgaggaagc tgcgaaaaca ggaagcccag caaagacgca
    15781 agaatcaatc gcacgaatca tcgccagcct ccgaggatca ttcaaactag ttgagcttct
    15841 tgaaacattg aaatttccta aagcaaccta tatgtattgg cagaaacggt ttgacagacc
    15901 aaacccagac caagaaattg aagaaaaaat gcttgaaatt cgcaaggagc ataaagacta
    15961 tggctgtctg cgactaaaaa aagaattaga gaatcaaggg attcatgtca ataaaaagaa
    16021 gattcaacgt ctgataaaaa aactaggtat cgaagtaaag tcttacactc gaaaatctcg
    16081 acggtacaat tcttatcatg ggaaggttgg cactgtcgcc aaaaatcgta ttcatcgtcg
    16141 gttttataca aatatctgtc accaaaaaat tacaactgat accacagagt ttaagtatta
    16201 tgaagtagac accacaggtg tcgttcgtca gaaaaagctg tatttagatc ctttcatgga
    16261 tttctacaat agtgaaattc tatcttatcg gatttctgaa aaaccaaacg cactagccat
    16321 aatggaagga ttagaagaag caatccagat gacaaatgat tgtccatttc gtagaacctt
    16381 ccattcagat caaggatggg cctatcaaat gaatgcttat aagaataaat tgaagcagca
    16441 taaaatcttc cagagtatgt ctcgtaaagg gaattgttta gataactcac ctatggaaaa
    16501 cttctttggt ttattaaagc aagagatttt tcatggagaa gtctatcgga gtttagatga
    16561 attgaaaaca aaaattgacc aatatatcta ttattacaac cataaacgca tcaaaaagaa
    16621 attaaattgg cgaagtccgg ttcagtttcg agaagctgta aaaacagctg tctaacagtt
    16681 aattttatcg tacaaaaaag gagtggattt ctccactcca taaaagtcta actttttggg
    16741 gtcgctacaa atgcttatcg agaatttgga gaagaaggcc tcttgcgtaa gcgaaataag
    16801 cgaagttatt ctgttcaatt caagatagat gcgatagagt tataaaaaac gggtgagcta
    16861 tcctatcgtg aagtggcgaa tcatttaggg ataaaccaac ctgcgttagt tgtcaattgg
    16921 atgaaaattt ttgttcaaaa ggattgcacg ggctctcaaa aatgaaaggg tcctcctcca
    16981 ttttgtctaa gaaagaataa aaggaagaaa caagcaaact gacgcctaaa gagcataatc
    17041 gtatcaaaga attggaaaaa taagtgcgtt tccttcagat tgagaatacc tttttaaaag
    17101 aattgaggaa gcttagaaaa caggaagtct aacaaagacg aacgaatcaa tcgcacgaat
    17161 catcgacaac ctccgaggat cattcaaatt agttgagctt cttgaaacct tgaaatttcc
    17221 tgaattaacc tttatgtatt ggcaaagacg atttgatcga agaaattcag atcaagaaat
    17281 cgaagaggaa atgcttaaaa taagaagtca atataaagat tatggctatt tacgtatgac
    17341 aaaggaattg tgggaccgtg ggttccatgt taacaagaaa aaagtacaac gtttgattcg
    17401 aaagctgagt attgaagtac gatcatttac gagaaaatat cgccgctata atacttatcg
    17461 tggaagaatc ggcatgatta caaaaaatcg aattcatcga cgcttttaca caagcaactg
    17521 tcatcaaaag gttacaacag atacgacaga actaaaatat tttgaaatgg ataaatacgg
    17581 tgccaatcgt gaaaagaagc tgtatcttga tccattttta gatatgtaca acagtgaaat
    17641 tttattttat cgcatttctg aaaagccaag tgcgttggct atcatggaag ctttagagga
    17701 agctatctgc gcaacggaat gtatctatcg tcgaactttt cactccaaaa aggatggacc
    17761 taccaaatga aagcttatag tcataagttg aaagaacata agatcttcca aagtatgtcc
    17821 cgtaaggaga cctgcttaga taattcgatc attgagaact tccttagtct attaaagcag
    17881 gaaatttatt atggaagaga ctatcgtatc tttgaagaat tgaagcaagc cattgatggc
    17941 tatattcatt actacaacca tgagcgtatg aaaaggaaac taaactggca gagtctggtg
    18001 caatttagag taatagcatt caaagctgtc taaaatcatc aagaaaagca aagaaaaacg
    18061 gagtgaattt tctccactcc gaaaacatct atctttttta ggtcactaca gaatcaattc
    18121 tgattcatca gacccataaa ttatagatcc taataattta taccatgctc tttttactca
    18181 tatttacatt tatctttggg tattttatgg tttaagttga tgtctgatct agttttattc
    18241 tggattaatt aacctaataa tccaatataa actccaacaa tactaaagac gaagatagca
    18301 agtataatcc aaagaacctt tacttttttc tttaatagaa agtaaacaaa gaatgttagc
    18361 cccaatgaga gaattcctgg aaaaatatca tcgagcattt gctgaacatt tatttctcca
    18421 ccttttgatt taaatacggc gggaatagta aagcgtacca gagatgaggt catggctcca
    18481 attgacatta aacctacgat agatgctgct aacgagaaac tttcaaggta attgttctca
    18541 aatgcttttt caatggcgtt tgctcccatc cgatatccaa tacgtaagca gccatatctg
    18601 ccaagaaaat gtaacagcgt ataaattgaa aaatacaata ttggacctaa aatgtttcct
    18661 tgggcagcta aagataagcc gatgccagca gcaataacgc gaaatgttcc ccagtaaaaa
    18721 ctatcaccaa taccagctac aggccccatc aatgcagctt ttatcgatga aattgtttca
    18781 gtttcaaatt gatctgtttt tgcattttct tcttcaagtg ctaatgacat acccataata
    18841 aaggttgaaa tatgaggcgt tgtattaaaa aattccgtat ttcggtaaac agcttcataa
    18901 tatttttcat cattcccttg atatattttg tttaaagcag gtaatataga aaaactatat
    18961 cccattcctt gcatatcagt gtatgactcg gatccctgta aagcaaatga acgccagaaa
    19021 accttgttta tttctttttt agttaattta gaatctccca taattatcac tctcccttta
    19081 aatatttaca ggattgcgtt tagtttggat aactaataaa gcaaccataa ttgcagcaac
    19141 agctacacca actacgggga ctcctaaata tgccgctaaa aggtatccaa tgaaaaaata
    19201 aggaactgat tttttgtcaa tcattccttg aattagcatg gcaaatccaa cagcggggag
    19261 taaaccagaa gctacggtga aaccggtcat taatttttct ggcataatat ttacaaaact
    19321 ttctgtagca ttggcaccaa aatatactac taaaaatgta agtactgcgg aaggtaagcg
    19381 aacgattaat ggaaaaaaat gccacttctg aaaagatgat aatttgcggt tttcaattgc
    19441 ttttctacct tgtgaaacgg taaaggagtt aatcgtaaaa gccaacatgt ttaatccttg
    19501 tgccaataca gcgaaaggca tcgccaatgt aacagctaca tcagcacctg ctccactttt
    19561 tattgctaga gccgttgcaa tagtaccagc tacacctaca tctggtggta gtgcagcacc
    19621 aatcgttaca atgccaataa acattgcttc taatgctgct cctatcaaga tgccttgatg
    19681 gagatcccct aagataagtc caacaacagg tccaactatt atcggtcgat tcatcataga
    19741 tgccccaaaa tatctttcat ctattgtcgt taaataccca actatgccaa ttaaaatgca
    19801 ttcaatcatt cttattcccc accttctaat aaatcttcgc ttttccagtt ggagtagcct
    19861 gcataattaa tttgaagccg gactccttta attttgctaa gtagtttttg tcttctgatg
    19921 tgatagccac atactcgtta actttctctt ttccctcggc gtaccgtaaa ccaccgatat
    19981 taattgaatc gacttcccct gggatagcgt ttacaatttc aaatgcgctt ttaggggaac
    20041 tagtgactat catcaaattt tgatttttcc ttttttcaaa ggtacttaaa aaatctgctg
    20101 aagtaataat atctagtccc gttgatacag gttttgctaa tgataaaatc atttgtttca
    20161 ttttgtcatt agcagaggcg tcatcaacta caataatgta gtcagcagat acggacttag
    20221 tccaagccat tgcaacttga ccgtggatca ggcgatcgtc aattcttaat agtttaatca
    20281 ttatcttcta cctccattaa ttctgaacag tttataatgc tcttttggct ttcagcaata
    20341 atcatggaaa ttttttctct caatttataa tcttcaatgt tcatacttaa ttccataatt
    20401 aaggacaaag tcatacctgc aattactgta attttttttg ttaatttttt ttcgtaaaca
    20461 taattcatcc aatgattatt tacgctacca ccaagtaaat cagtgatgac aaggacttta
    20521 tcagttccat cgagaagaat attcgtatct ttttcaatat catagttatc gtctgtgtat
    20581 gcagtcatta cttttatgaa tggtgcaggg ccaaggatat attcgactgt ctctttcatt
    20641 cctttcgcta aatttttgtg gcttgcaaga ataattgtgt acataatcca cctccattta
    20701 tttataaata atataccatt ggtaatctaa atgtcaataa attatttcgt attaagaata
    20761 aataaaatac ctttggtaat tagtggggcg aatctaaaaa ctttaactaa aattctcttt
    20821 tgtaaatttt tttgaacgcg gctatcgtgg tgatttttta tttctgtttg tttaatgaat
    20881 ttgtaaatta ggaggtgatt tttgtgatgg tgtaaacaaa gcctataaaa atgaagtaaa
    20941 gcattgtgtt ttgtccagat gtcaaatctt ttatgtatcg gttgtatgta tagtatgtta
    21001 cacccaaaat agataaccca agtccaaatg gagagacaga tgttctttat attactggcg
    21061 aagacatgaa gtattcttat aaaatatcta aaaatgagta taagtttaaa gaaaataatt
    21121 tatctatata aactattctg ctttgattag caagtctttc aatcggtaaa atattatttg
    21181 aatagatggg atatggttta ttattttcta aatgtcaaat taagttagac aattgctcct
    21241 cacttatgta actcaaaaat acttctaacg atgttcgata atttaaagat ttccttggta
    21301 tatgatttct tttggaggca accgccgcca caaatttttg gtcaacctga ttgaaatcca
    21361 ttgtattagg aagtccgtct ttacgtaaca atccattaga atgttcattt aatccccgtt
    21421 gtgacagcgt tccaggatct gcaaaataaa tagaaatatc gttagcatta ctcatgtttt
    21481 tccaattcga aaattctttt ccgcaatcaa aggtaatgga tttgaacaag tttcggggaa
    21541 gcttttgtag ccagtcagtt gtcacttttt caatatctag tgctttccgt ccttcgggtt
    21601 ttaaagtaat aatcactttt gacaagcgct caaccagcgt gatgacagca cttttatgat
    21661 ggacacccac aatagtgtcg ccttccaaat gtccaaattc ctgttgaaat tcaggatagt
    21721 ccgtgagacg ttctgaaata ttccgtttga aggcttgttt ccctcgacgt tctttatgcc
    21781 cgttgggttt ccgttttcct ttcatgggta gtttcgagca atcaaattag tttgttttaa
    21841 acttgcgata aagggttcga acagaacatg aaattgaaaa ctcagcacga ccgataatca
    21901 catcgggtgt ccatccttgc gccaccttgt ttcggatata cgcatcttgt tctgctggca
    21961 aacaaatggg tcgtcggccg caccgtgttt tatttttttg atattgttgg taatagtcat
    22021 ggatagattg gccttgtttc aggaagttgt acacattgta gatggtctgt ctggaacgat
    22081 ttgaggtctt agcgactacg gtaacagatt gatgcatatt gaaataggct tctatcatca
    22141 cgagttcatt tgttgtaaga tgggtatagg tcatttgtct tcactcctta taattctttg
    22201 gtcggaacta ttttgagtgt agcacaaatg acttttttag ttgtctagct taattttaca
    22261 atctgcgttt aataaactct cagtgggata gtttgactcc gtatatgact ccggttgatt
    22321 tgattgaatt cagatgatta tacatactat aaatttctct attatgttgc catttgattt
    22381 gaataatgct gaaagaagga ttttagtcta taacgaacaa gggaataaaa ccaataaaaa
    22441 aagatataga atatcaatat aacaaaaaga gattgtgaca taattctctg aaaaaagctc
    22501 tagaaatgac gagtcattcg tcatttctgg agctttttag gtaaaataaa agagtacgag
    22561 aggatactcg cactctaaat cattcaaccc ttgaaaggat caaaatcatg catacaaatt
    22621 ataacatgaa tcaactcact ttagatatca caacttctta ttccaaaaga agatagcacc
    22681 gctgggtttg tcaatgaact cgttgaaacc cttgaaatca acgagccgta tctttttggc
    22741 cgccctagga attgtgattt gagtgccatg ttaaaattgg ttcttttcgc atattgaaaa
    22801 tgattgaaga ggactatcgg tattacaaaa gacaatataa ccagcaggtg aaaaaatgga
    22861 gaaggtaatt ccgaaaaaag cttcttttgg cgattgggaa taattgttgg taatggagga
    22921 ataaaatgaa tgtttatgca gacgtttgat gtctacatca acatccattc ttaatactcg
    22981 aaggttcgtt gttgaaaaaa ctaacttttc ttcaacagag gatttacgtg caaatcaagc
    23041 cgagtatttc ttcaataatc tttgtttgac gaaactgtat aaggcagtaa agaaagcaag
    23101 tcctaatgtt aagtagccca acggaacaca aaagaacgct ggttcaatca gcattccgct
    23161 tgaatcgagg taagaaccga ctaggatgga agttgaaaat aacaaaatac tcaagaaaag
    23221 aaatatacaa ggaataatga attttttcat ggtgtctcct tctttgtttt tctagattat
    23281 atagaataga acctaggaaa agataagtgt gacaaaattg ataggtgtga aaggcattga
    23341 gatatcaacc agctttatcc tttggagtat gctattgtga ttaatgagac agaggatgtg
    23401 ggaccagacg atactgaatc aacggaaatt gttctaaacc accatcaaat agtagttttt
    23461 ctagtatttg aaggtgattt ttttatgttc gaaagtaact gtaaaaaaga taaataatcg
    23521 gcagttattc tttcacataa gtaggttgga tgcaggtgag agaagtttat tgtatgagag
    23581 gaagattggt aatctaacta tagttttata actaatatca gtttaagctc gattattttt
    23641 gtttcaaagt aaaattggaa cagaaatata gaatagacat attgtagccg atagtttatt
    23701 atgcttctag acacgttgaa ctggaggg
//
LOCUS       DAZUMV010000032        23476 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000032 DAZUMV010000000
VERSION     DAZUMV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.032"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..1438
                     /locus_tag="ACG8FK_001133"
     CDS             11..1438
                     /locus_tag="ACG8FK_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5096753.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1530..1895)
                     /locus_tag="ACG8FK_001134"
     CDS             complement(1530..1895)
                     /locus_tag="ACG8FK_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5096754.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1892..2257)
                     /locus_tag="ACG8FK_001135"
     CDS             complement(1892..2257)
                     /locus_tag="ACG8FK_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5096755.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2449..2697)
                     /locus_tag="ACG8FK_001136"
     CDS             complement(2449..2697)
                     /locus_tag="ACG8FK_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096756.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2696..2824
                     /locus_tag="ACG8FK_001137"
     CDS             2696..2824
                     /locus_tag="ACG8FK_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS5096757.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2881..4575
                     /locus_tag="ACG8FK_001138"
     CDS             2881..4575
                     /locus_tag="ACG8FK_001138"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS5096758.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4675..4971
                     /locus_tag="ACG8FK_001139"
     CDS             4675..4971
                     /locus_tag="ACG8FK_001139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096759.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5349..6482
                     /locus_tag="ACG8FK_001140"
     CDS             5349..6482
                     /locus_tag="ACG8FK_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS5096760.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6513..6833
                     /locus_tag="ACG8FK_001141"
     CDS             6513..6833
                     /locus_tag="ACG8FK_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS5096761.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6862..7326)
                     /locus_tag="ACG8FK_001142"
     CDS             complement(6862..7326)
                     /locus_tag="ACG8FK_001142"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5096762.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7457..8710)
                     /locus_tag="ACG8FK_001143"
     CDS             complement(7457..8710)
                     /locus_tag="ACG8FK_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5096763.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8739..9056)
                     /locus_tag="ACG8FK_001144"
     CDS             complement(8739..9056)
                     /locus_tag="ACG8FK_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5096764.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9082..9402)
                     /locus_tag="ACG8FK_001145"
     CDS             complement(9082..9402)
                     /locus_tag="ACG8FK_001145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5096765.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9866..11281)
                     /locus_tag="ACG8FK_001146"
     CDS             complement(9866..11281)
                     /locus_tag="ACG8FK_001146"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5096766.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11298..12614)
                     /locus_tag="ACG8FK_001147"
     CDS             complement(11298..12614)
                     /locus_tag="ACG8FK_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5096767.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12764..13462)
                     /locus_tag="ACG8FK_001148"
     CDS             complement(12764..13462)
                     /locus_tag="ACG8FK_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS5096768.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13751..14744)
                     /locus_tag="ACG8FK_001149"
                     /pseudo
     CDS             complement(13751..14744)
                     /locus_tag="ACG8FK_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(14825..15658)
                     /gene="msrA"
                     /locus_tag="ACG8FK_001150"
     CDS             complement(14825..15658)
                     /gene="msrA"
                     /locus_tag="ACG8FK_001150"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5096769.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15892..16635)
                     /gene="truA"
                     /locus_tag="ACG8FK_001151"
     CDS             complement(15892..16635)
                     /gene="truA"
                     /locus_tag="ACG8FK_001151"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5096770.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(16826..17461)
                     /locus_tag="ACG8FK_001152"
     CDS             complement(16826..17461)
                     /locus_tag="ACG8FK_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5096771.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(17608..18828)
                     /gene="sstT"
                     /locus_tag="ACG8FK_001153"
     CDS             complement(17608..18828)
                     /gene="sstT"
                     /locus_tag="ACG8FK_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS5096772.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            complement(19170..19847)
                     /locus_tag="ACG8FK_001154"
     CDS             complement(19170..19847)
                     /locus_tag="ACG8FK_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS5096773.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(20098..20640)
                     /locus_tag="ACG8FK_001155"
     CDS             complement(20098..20640)
                     /locus_tag="ACG8FK_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS5096774.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(20645..20872)
                     /locus_tag="ACG8FK_001156"
     CDS             complement(20645..20872)
                     /locus_tag="ACG8FK_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS5096775.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(20951..22831)
                     /locus_tag="ACG8FK_001157"
     CDS             complement(20951..22831)
                     /locus_tag="ACG8FK_001157"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5096776.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            23132..23452
                     /locus_tag="ACG8FK_001158"
     CDS             23132..23452
                     /locus_tag="ACG8FK_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5096777.1"
                     /translation="MDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLKLLIHT
                     ACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECFFIYDI
                     IASK"
BASE COUNT         7275 a         4821 c         3965 g         7415 t
ORIGIN      
        1 aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc tgtttttatg
       61 tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc tcctgcatgt
      121 ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg aagaacagat
      181 cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac atatacctcg
      241 atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt gtctgcttta
      301 tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca tctctgtcag
      361 caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg ggatcgaaat
      421 cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc ttaatgtgtc
      481 gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg aacgtgccga
      541 tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag aaaataaaga
      601 aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg ctgtgacaat
      661 agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg tcgaatcagc
      721 atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct ttattttgac
      781 actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg aagactttca
      841 acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag ttgctacctt
      901 tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg cgctgattca
      961 tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat tttatctttt
     1021 aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca gctggtccag
     1081 ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg tgatcgaatt
     1141 gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca caaattcgga
     1201 tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg attttcaagt
     1261 tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc agtcctcaaa
     1321 ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg aaaatattat
     1381 tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa acaaataagg
     1441 ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat ttctgtaaca
     1501 atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac gggtccgatc
     1561 tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc tagttggaaa
     1621 atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac aagaatttcc
     1681 aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc gctgactcgg
     1741 tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat tgatagacaa
     1801 acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag tttttcgaaa
     1861 tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt ctttgttttt
     1921 ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg ataataataa
     1981 gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg atatcagcaa
     2041 cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt gtatctgcat
     2101 tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa agtttgaata
     2161 gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa atagcaatga
     2221 tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac tttcttttaa
     2281 tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt aaaagttgtt
     2341 ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc tccctccatc
     2401 cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt agtccgtcat
     2461 cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc taaaaataga
     2521 accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta caggaaaatc
     2581 agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca tttggcattc
     2641 taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt ttttcatgag
     2701 taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg ctgcatatct
     2761 aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat tcgaatcaat
     2821 ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg aggaatcaca
     2881 atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc agcggctgtt
     2941 tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc attggatgat
     3001 cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg gaataaaaac
     3061 catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct taattaccgt
     3121 acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga gccagggatg
     3181 actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga caaagcacgt
     3241 ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa taataaagac
     3301 cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa actagacaat
     3361 atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa cttctggttt
     3421 atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt gcgtcgctat
     3481 atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt caatcgaaca
     3541 cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa agaacaagga
     3601 tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac accgatgcaa
     3661 gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga agaagaatac
     3721 gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac agattcagct
     3781 actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc agcttctagt
     3841 ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa attctttgct
     3901 gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca tttgttgttg
     3961 aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc atttatttca
     4021 accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt cgtgcatcat
     4081 caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta tttcctttat
     4141 ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg aaaagaaatg
     4201 ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa tattcgtgaa
     4261 aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat gccttatgct
     4321 tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc taaacattcg
     4381 accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat ctttactgaa
     4441 caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat tcctatgtcc
     4501 cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg tgccgcaaaa
     4561 aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     4621 accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc gcagatgcaa
     4681 gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca agtaataaga
     4741 acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt accgcagatg
     4801 caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca tcaagtaata
     4861 agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt attaccgcag
     4921 atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta gtttcgagta
     4981 tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga cttaataccg
     5041 agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt tttgaaaaca
     5101 actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga tacaactatt
     5161 tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac aaatacataa
     5221 gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg taagatctcc
     5281 tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat ctgaaaggag
     5341 catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc agaaacgtag
     5401 atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca acagccattt
     5461 gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa gggtatgttg
     5521 taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa acattgaaaa
     5581 aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat ttctgcctga
     5641 atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc gggcttttcg
     5701 gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta agagttcctt
     5761 atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa aaagttttat
     5821 ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga gtaaaaaaag
     5881 gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa aaattcgctg
     5941 ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt ctagataaag
     6001 cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa gctggaacag
     6061 aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta ggaatggacg
     6121 gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc ggcacaatct
     6181 ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa gtaactggtg
     6241 tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg gatatcgctg
     6301 tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca atgattgcag
     6361 aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca gaagcagcac
     6421 aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt ttgaaacctt
     6481 gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga aatacccatg
     6541 agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc cgcttctatt
     6601 tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg attttctata
     6661 tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc tatttttatt
     6721 tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg aacattttta
     6781 tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca tgagaatttt
     6841 tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac gagcggtcgt
     6901 cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat aaaacgtggt
     6961 atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt ttctgatttc
     7021 cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa acagggcaca
     7081 aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc ccgctcctcc
     7141 gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt ccttattagc
     7201 gattccagta cgaatacttt cctcatacac aacagcagga actccccatt ttgatgaaaa
     7261 ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc agttatatgt
     7321 aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac aagtctttat
     7381 gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa tcacttgttc
     7441 tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg atcgtagcta
     7501 ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg gctagtacac
     7561 cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt acccatggag
     7621 gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca acactaacta
     7681 agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg ataggtaacc
     7741 caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag tttgttacaa
     7801 ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa cctaaagttg
     7861 cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg ataaacggtg
     7921 cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca ttgattgttt
     7981 gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag aatggtgtga
     8041 taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat ggttcttgga
     8101 ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa attgcagcaa
     8161 ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat gcttttgcaa
     8221 ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg cttaaacgta
     8281 caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg tttgagctca
     8341 tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct gcggaaacac
     8401 cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc aagacagaaa
     8461 gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat tcaccaccaa
     8521 aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc attactgcta
     8581 atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc gcaactaaat
     8641 gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata aatgcaacaa
     8701 atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa tcgacgatac
     8761 agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag atgatcttga
     8821 ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg tgtcagcatc
     8881 tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat tttttcatct
     8941 gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc gctttttgca
     9001 tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc ttccatttat
     9061 tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt cgagtacttt
     9121 ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa tgcctttagg
     9181 tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta aaagtaaaac
     9241 gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag caaagatgtc
     9301 tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga gacttgtact
     9361 cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca cgttcctttc
     9421 tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct ttaaaaagtt
     9481 gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag aaagcgcaca
     9541 ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa aaaaagttgt
     9601 gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt gcatctgcgg
     9661 taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta ttcttattac
     9721 ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca gctgaccctg
     9781 ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc agtaaataca
     9841 atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa tccaattctc
     9901 tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg cttcgttcat
     9961 cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt ggtgacacac
    10021 agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct gttgctttga
    10081 tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc acgattcctt
    10141 tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt atcagccaag
    10201 ggatattatg ataattttct tttagataca tggctatatc atacatgatt ttcggataga
    10261 tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact tcataatatc
    10321 gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga acgcgtttag
    10381 gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt tctaaatcct
    10441 ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac tcttctgtgt
    10501 agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca tacatataag
    10561 cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga tttaaaatac
    10621 agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta gccgttgcta
    10681 aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc ggccagcgaa
    10741 cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta aaccagtttt
    10801 ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa aaatcaacta
    10861 cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact tcataatgtt
    10921 ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt tcacttatat
    10981 gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa cgagcccaat
    11041 tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca tcctgctggt
    11101 attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa acaaaatgtt
    11161 catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct ttccaacctt
    11221 ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata gtaattttca
    11281 tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac ttttacaaat
    11341 ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac tgcaccgata
    11401 tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg gaccattaca
    11461 actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc agtgataaga
    11521 ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat accaaaaata
    11581 actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat ttgtttcata
    11641 tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac tccagacccg
    11701 cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac ttcttttcca
    11761 gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc ccgtatcggc
    11821 ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat aaacatcaat
    11881 agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac gacattgtaa
    11941 atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc agccgcgata
    12001 cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac attcgttggc
    12061 acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca ataaagttca
    12121 atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt atattgttta
    12181 gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata aacagttgga
    12241 ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc cactttattc
    12301 agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa acttagtgtg
    12361 acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa gaaacttatc
    12421 tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc aaaagcaccc
    12481 gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat ataccttaaa
    12541 ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat aaaactcatt
    12601 ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc tattagtcag
    12661 cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat ataaaacaaa
    12721 aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta gtttcttttt
    12781 ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc attgcatcaa
    12841 acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga atatcgatcg
    12901 tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt gattcattaa
    12961 tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc atcgtatgaa
    13021 aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt tcaatctttt
    13081 gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct gccaaacctg
    13141 ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc cgatcctgat
    13201 cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca ctgacaaatg
    13261 tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc acagcttctc
    13321 gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc ggcagtttat
    13381 ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta gttacttcta
    13441 ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta ttttttcgta
    13501 attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg attaatccgt
    13561 tcatagacat acaaacgaag aagatattta ccataaaaaa gactaggata aaattcactc
    13621 ctagccttcg atcattttta ttttgaacga caaaaaatat gatgttcaat aacagacgat
    13681 cttcattcga ttcttgatgt catcttttgt tttttttaat atatggataa aaagtttgat
    13741 tacatcgttt ctattctaga atttggtcga ttctcaattc ttcttctggt gagaaatcta
    13801 aattatccat cattcgaaca ctttcttgta actgacttaa ccgacttgct ccgaccagta
    13861 cacttgtaac agatttttgt cgcaaattcc atgcaagggc catttgcgct aatgattgtc
    13921 ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc cagagttgat ccaacttgtt
    13981 cttctgaaag gaatggtatt tccttacgat gtgctctaga atcttcagga ataccatgta
    14041 gataacgatt tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc cctagccctt
    14101 cttcttctaa tactggagat agatcgtctt ctagtagtgc acgagaaaac atattgtagc
    14161 gcatctgatg aataatgaac ggtgcttcct ttctttttaa aatttcagtc atttttttgt
    14221 atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc ttacaagttg
    14281 catcaacgct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat gatgatagaa
    14341 gatgtcaaca taatctagcc ccattctttg gaaactttgg tcacaacttg atatgatgga
    14401 tttacgagag ccccattccc catatggccc ttcccacata tgataacctg ctttgctaga
    14461 aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc caaagttttt
    14521 ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg taattcccat
    14581 gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat caccaaaatt
    14641 ttgccataat ccgagaggag agataaaatc ggtaatttta aacctgaatg accggattta
    14701 cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc tcctcctcat
    14761 caaatctttt taaaacgctt tcttgttttc tatttttagt ttactacatt ttaaagaaaa
    14821 ggagttaatc aaaatgattt tttcgtatat ttttttgcca tttgtttttt actcgttgat
    14881 agatcagaaa ttgattacgt gcttgctgga tttttttata gcgtttcggt tgttttttgt
    14941 aaaattgctg atgatagttt tctgctggcc aaaacgtacc ggctggttcg atttttgtga
    15001 cgattggttg gtggaaagtt cctgaactgt ctagtttctg ttttgattgt tccgctaatt
    15061 ctttttgtcg ttcgtcttga taaaaaatca ctggacgata ttgcttccct ctatcttgaa
    15121 attgtccaaa tgtatctgtg ggatcagtaa tttgccaata gattgtcagc aactcgctat
    15181 aacttatttc tcttgtgtca taaatgatct ccacagcttc cacatgacct gtgtaaccac
    15241 cacttacttg atcataattc ggtttttcaa catgtccgcc cgtgtatcct gacaaaacag
    15301 ataggatccc ttttttcgat tcaaacggct cgaccataca ccagaaacat ccccctgcaa
    15361 atactgcacg ttcttgatag tcagcatctt gcatggcatg cttggaaaag tcatattttt
    15421 gctccttatc aaatccttcc gtaatcaata aataaaagtc cattacatca ggcgtcagat
    15481 tcattcggat agctaatgga cgtagtgtta cttctagttt tgataactgt tctttaacgg
    15541 atatattttc tcttgtgtca ttctttgtct gaatcaatac ttttcgttcc caatctcgtg
    15601 tattcggatt caaaatcaga ttataaagtg tttgaatgaa tatttgttcc tcatccatag
    15661 attctcctcc tttccgaaga agacaagcta gtgactttag ctgtaattta tcataaagtg
    15721 aggaaataat cgattgaatc gtatttattc aaagaagata agcagcttca gatgatataa
    15781 gatgatcttt atcttaaaaa gcatactcta tggtttggtt taactaaaaa acgacctctg
    15841 aaaaaaggga tgttctccaa ttctcagaag tcgagtaaag atgattcttt tttatgagcg
    15901 ataataaact tcatccaaaa acagaccttg tgcagggacg gtttctcctg catgttgccg
    15961 cactttgctt tcaaatattt catcgattgt ttctatcggc atggcacctg tgccgatttc
    16021 tagtaatgtc cccatgataa tccgcaccat tttatataag aatccgtctc cgacaaaagt
    16081 aaaatgaagc atttctccct ttcgttcgat agataattca tcaatcgtac gcactgtgga
    16141 tttttttgat tttttcaatg cagaaaaacc aatgaaatca tgtgtaccca ctaatttttc
    16201 gcaagctgca ttcagcttgt ccatatccaa ctcttgaggg aaataaaagc tgtggtttcg
    16261 ctcaaatgct gaaggaatca cgttgttcca aacataatag ctgtattttt tgcccaccgc
    16321 attatatctt gaatggaagc gctcaggcat ttcttcgatt cttttgatga caatatcccg
    16381 cggaaggtaa cgattcataa agtcgatcat ttctgccagt ccaatttctg tgtttgtttt
    16441 aaaatttgct acttgtcctc gcgcatgggt gccagcatct gttcttcctg atccaataat
    16501 ttctatctct tcatttgtca tctgatgcaa tactttttcg atttttcctt gaatggtttt
    16561 atcgtcatct ccaagtcttt gccatcctga atatcttttt ccgtcatatt cgattgtcaa
    16621 cttgatgttt ctcatatttt agtggctcct ctgtcttcca tttggtcttc tctctacctt
    16681 tttaagattg tagcacaaaa tatagctcct tgcttctcta agacagaagc tatacaagaa
    16741 aactgtggaa atatatattt cagaaaaaca aaaaacagcc accggatgaa gtatccgctg
    16801 actgttcaat ttgacggctc aagttttact gtttgagatg caagatactc aaccaggcaa
    16861 tgggcacata attaaagaaa aaactgattc tttggcaaaa acctatttta ttcagtaatg
    16921 gccaagaatt tatagaagga attcttgcca gtccataaat cgccgcaaaa agcaaactga
    16981 tcacagacag tccaaggtat aagtggctta cgtccatttg cccatttttt cgatagatca
    17041 agaaaagaag aactggaaac aataaaaaac cactgtaacc taaacctgat ccgatattat
    17101 ggacccatgt cgagacattc cacgaagatt catgggtatt gatactgaac aaacctgtaa
    17161 agatacaatc acctatacca tagaacgcga tggctgctgt aactagggta gctaacacac
    17221 gagatgtact tacaaatgca gcatacaatg caggcaatga caggatgaaa aagatccctg
    17281 aaaccacaga ccaaataaga aacgcttttc ttacaggact ggaaacctct ccaaatacac
    17341 tgatcacgct cttcatttga tctaattcag gataatacaa tcctaatacg tacggagtca
    17401 aaaaatcact cagcacgcct aataataaaa agaaaaaacc aaatttacgt aaaaaatcca
    17461 tgatacgcct ccaataacaa ttacctatgc ttttaattgt tattataaac gactttatct
    17521 tgatttaaca gaaacctcta tttacacgaa catctatata actcattaaa aactcgaatc
    17581 attttaaaaa tactaaatat ctaacgttca acgttcactt aatccatcta aacaatcttt
    17641 caatttgatt ttttccccac ttcttttacg cgcgcctagc tctcctattg cagtaaacaa
    17701 cagatcactt gacgaattta aggcagtttc cattgaatcc tggataacac cgataataaa
    17761 tccaatacca acgatttgca tcgcgacatc attagagata ccaaacaaac tacatgctaa
    17821 tggaatcaat aacagtgaac cgcccgcaac acctgaagct ccacaagcag ctaaagcaga
    17881 caaaatacat aaaaggactt tcaaaacaaa tggtgcttca atccctaaag aatgtacagc
    17941 agtcaacgtc atcaaggaaa tggtaatcgc ggcaccgccc atattgatcg tagcacctaa
    18001 cgggatcgat atggaataag actcttttgt caatttcaat ctttcagcca acatcatgtt
    18061 tatcgggata tttgcggcag aacttcgggt aaagaaagcg ggaagtgcgc tttctttcaa
    18121 acaaaagaaa atcaatggat agggattttc tttaataaac aaaaacgtga ggaatgggta
    18181 cacaactaat gcgacaaaca gcatcgtacc gatcaaaaca agtaaaagct gcagatactc
    18241 tgccaatcca gcgattccag tcgtagcaat tgattgataa accaatccta aaatccctat
    18301 cggagcaaat gcaataatca cttgtacgac ttgtgacaac gctatagata tctgatccac
    18361 caactctttt gtcggttctg aacgtgctcg aaaagctagt cccaacaata cagcccagaa
    18421 taaaacgcca aggtaattcg catcaataag agattgaaca ggattttgtg tgacattcgt
    18481 caacatagta gataaaacac tttccaaact ttgcggtgct ttttcagata ctgtttcttg
    18541 taaagtaata ggaacagtaa aaattgaact tacaatgact gctacaagtg cagaaagcaa
    18601 tgttcccact aaatataaga tcaaaatagg cttcacgaat gtcttattcc caattttatg
    18661 cttggcaatt gacgccatcg tcaagaaaaa aaccagaata ggtgcgattg ccttcaagca
    18721 actgacaaac aacgtcccca atattgaaat aaatgtccac gtggggaaaa agataccaaa
    18781 caatattccg actaaaatac caacaacaat acgttgaact aacgacatat ccaaaaactt
    18841 ctctaacaaa tgaccaccct ttcacaattt taacaatcat tgtatcattt tatctaaaga
    18901 aactaaaggt gcaattttct aaaaattcta ataatacaat tggcaattca tgctcttgca
    18961 acattcaatt ttagtattgg agggtttatt caatctctaa agaagtcgag agaaaactat
    19021 ttatcgttat tattatagtt atccaagtaa aacgtaacta agatgaagga cgaaactaga
    19081 aatttcatcc ttcatcttaa ttcttagaca tagcaatcaa ttaatatttt ccactgagtc
    19141 tcttctttct ctcactctca ctggtttact tacccataaa cgatttgatt aaagccattt
    19201 ttcaattcat tattcgctgt ctcgttaaag taaagataat tatcgggatt cccctgattt
    19261 tgttgtgtca ccaatacttt tggctgattt tgctgaaacc cgaatagata gacgtgcttt
    19321 ttcagatatg gttctgtctc tgcatcggaa tagacggctg tgatttgata gtctgcttga
    19381 cctgtcccac tttctgacca ttctgcagaa acaggcactc caccaatagc catcttccac
    19441 tccccgttca aaataacttg agggacttta agaccatata agtccacact catttgattc
    19501 gtatagcttt tgtattgttg tcccattgtt tggccccatg tagccataaa tgcctctaat
    19561 tgtgcggatt tgtctgcatt ccacagtgtt tgttcgactt gttcgctgct ttctgaagat
    19621 gaagcctcta cagaagaact tgaagtagat gtttgagttg tttcagtcgt tttggaagac
    19681 tctgacgaag tagaggtatc tgctgtttcc gtacttgctg cagttggctt gctctctgtg
    19741 ctagttgttg tagtagttgc aattgttgct ttcgctgttt cttttgttga ttcttttttc
    19801 gattcattcc cgttggtaca accagctaac aaaaaagcta gaccaattgt tactaaaatc
    19861 ctttttttaa attaaaaaac tcctttcgat tactcccact tctttattct atcaaataag
    19921 caatgtgatt ttattgattt tttgtatttt ctcatccatc ttttaaacct tttaaaaact
    19981 aagaagcacc cgctgatgtt gtctgtaata catcagcggg tgcttctttt attttaatct
    20041 tcgcgatttt ccaatttacc tcacgcttta tatttgtatt tgaagaaaca tagactttta
    20101 gtcatcatcg tcttcctcat ccaaatcttg tcggacagtt tttccttgga gtctttgtac
    20161 ttggtagatg atttctttat tgaaacttaa aagttcaaca agcaattgtt tcaagccata
    20221 tttttcttct ttttcagcta atttgattgc ccgtgtgata aagacatttt ggaaatcaaa
    20281 atcttttgca aagttttcca acattgttga ggctggtaaa tatttgtttt gaccatgttc
    20341 gtaaattttt ccataacgca tgacttcttc taaagtagac ggaacgattt cattttcaat
    20401 cactaactca tgagagattt tattgaacca tctcgcttct tgatcagcat attctgaaaa
    20461 aagggtactt aattcttgcg aagatgtacc caaaacatac aattgtgctt ggatcaattt
    20521 catttcttct aatttaaaat ttgccaatac atgccccacc atggcgccag cagttggttt
    20581 atgatgatcg atatctgctt gttttgtttc ttgagcaaat ttttcttcca ctatattcac
    20641 tgatttacgc ctcttttact tttgttgatt ggacttcata acctaatttt tccacaactg
    20701 cagctaattg ttcagggtgg acttcatctg gatcaaagtt tgctttgatt ttgctcgcat
    20761 taaacaaaac tttcgtattg cttaccccag gctgttgaga aaccgcatgt tctatttttt
    20821 gcatacagct tggacaagct agtgtatcta attgcataat catttttttc atcgtagttc
    20881 ttccttcctt tttcttgata gtcttattct agattacagc ttccataaaa aaattgattc
    20941 ttatcaagtt ttacgatttt tcttctttgc tttcgtgtcc aaaatacaat aaacgcatcg
    21001 cgttaaagat aacgatcaag atactagctt cgtgaacgaa catcccacta gccatgtaaa
    21061 taaacccggc aaataagccg atgagtaaaa ataagacgac accaatggca atcactacat
    21121 tttcacgggt attcaaaaca gttttcttag ccaaacggta agcatgaacc aacgatccaa
    21181 aactagattg catcaagaca acgtcactcg tttcgatagc gacatccgtt cctgatccca
    21241 tagcaatccc gatatcagcc gcagccaaag agggagaatc attgattcca tctccaatga
    21301 aagcaacgtg caatccttgt tcttggtatt ttttcacaaa ttgaaccttt tcatctggca
    21361 ataattcagc atggacttca tcaatgccta acatgtcagc tacataatca gcagtcatct
    21421 gattatcacc tgtcagcatc actaaatgtt ttgctcctgc ttgtttcaac atagctaatt
    21481 gttcagcgac ttctggtcga atcacatctg acacaccaat gatttgagtg atttttttgt
    21541 cgattgctac aataatgact gagctgcctc tttgttgtaa ttgatgtaaa tcttgtactt
    21601 gctttggtgt gagagaaagt ttgtgagcgg caatcaattt ggtgttgccc gcacaaactg
    21661 tatgaccatc aatctcagct gtgattcctt gtcccttgat cgtctgatta tctttgactg
    21721 ctaattgttg aaaatcaatg tctttctcct gagcataatg gatcacggcg cgccccagag
    21781 gatgatcgga taacgtttct aatcgagcca ctaaagctaa gctgtcccct tcatcctctg
    21841 tatagttctt gatagtggat acagcagtag ttcctttggt caatgtaccg gttttatcaa
    21901 agacaaaagt atcgattttg gaaaaagtat ccattgcttc accgcctttg actaagatgc
    21961 cccgtttcgc tccattaccg attcctgcta cattcgaaac aggcgcacca atgactaaag
    22021 cgccaggaca accaagaacc aaaacagtga tggctaaacg aaaatcacga ctgatcagac
    22081 caacaagcaa cgcaataagt aaaacggttg gagtataata ctgagaaaat tgatcaatga
    22141 atttttccgc atgtgatttg gtatcttgtg cttcttcgac taattctata attttggcaa
    22201 aggtcgtatc atctccgacc ttagttgctt cgatcgtcac gtagccatct gctaaaatcg
    22261 ttccagaaaa tacgttatct ccactgattt tgacgacttg tcgtgattca cccgtcactg
    22321 cggcttcatc cgcataccct ttcccgtcaa tgactcgtcc gtccacagga atagctccac
    22381 cagtttttac taagacgata tcgccttctt ccacgtcatc gatttccact tcttcggtcg
    22441 ttccatcttc ttggacaacg ctggcagtcg atggggccat ctcggtcaaa gccttgattg
    22501 attcccgcgt tttggtcaat gttttttgtt ccagataact accaaacaaa aacaagaatg
    22561 taacgatagc tgactccttg tattctccga tcataaaggc accaagcaca gcaatagtaa
    22621 cgagtaaatc aattgagata actttgactt ttactgcctg atacgcatgg attataatag
    22681 gtatgaccgc aataatagaa gctatggcta gagcaatgtt atagaacaag acaaatccag
    22741 taaatttccc gataaaacca aaaataatca acacagctga taaggcggtg atatgatttc
    22801 gatgctcttg gatccacttt tgtaatttca tctttctccc tcttttcctt ttcattctta
    22861 cgagactaag tatagatact tatccatcat aaaaaattga ccactatcaa gtttattcaa
    22921 aaaggcggtt ttcttttggt atactgaatt cctacagtca ttaaacgatc atatagaaaa
    22981 aggaagcgcc gaaacgcttc cttaggtagt tctatttttt tattcaaaga taagatgctt
    23041 tgtcatctta cgactgaaga acaacaatca aaaaagggca gcagccactc attcacaaga
    23101 aatatcgttt tttgatatgg gagttgaagc agtggacaaa tatctctact tattagctgg
    23161 gaataagatc caaaaaagct taatggattt tatccaagaa ttggaatgta ctttccacaa
    23221 aaaattcact cattccattc ttttaaaact tttgattcat acagcgtgtc taattgagcg
    23281 tacattgata aacggtcatg aacttaaaat tatatcagaa gatgacacaa gaccttctca
    23341 tgaaacaatt tttcatgtaa aaaaggcctt caaaaatatt gaaacagaat ttgggatcac
    23401 tgtttcttat gatgaatgtt tctttatcta tgatattatc gcatctaaat aatctttttt
    23461 tatgtaaacg agtatt
//
LOCUS       DAZUMV010000033        22853 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000033 DAZUMV010000000
VERSION     DAZUMV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.033"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..572
                     /locus_tag="ACG8FK_001159"
     CDS             <1..572
                     /locus_tag="ACG8FK_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS5096778.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="ACG8FK_001160"
     CDS             576..1217
                     /locus_tag="ACG8FK_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096779.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="ACG8FK_001161"
     CDS             1214..2131
                     /locus_tag="ACG8FK_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5096780.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="ACG8FK_001162"
     CDS             2128..2799
                     /locus_tag="ACG8FK_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096781.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="ACG8FK_001163"
     CDS             complement(3340..3828)
                     /locus_tag="ACG8FK_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS5096782.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="ACG8FK_001164"
     CDS             complement(4029..4295)
                     /locus_tag="ACG8FK_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5096783.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="ACG8FK_001165"
     CDS             complement(4295..4558)
                     /locus_tag="ACG8FK_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5096784.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5079..5219
                     /locus_tag="ACG8FK_001166"
                     /pseudo
     CDS             5079..5219
                     /locus_tag="ACG8FK_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..7184)
                     /locus_tag="ACG8FK_001167"
     CDS             complement(5361..7184)
                     /locus_tag="ACG8FK_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5096785.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="ACG8FK_001168"
     CDS             complement(7782..10487)
                     /locus_tag="ACG8FK_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS5096786.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLQIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="ACG8FK_001169"
     CDS             complement(10793..11740)
                     /locus_tag="ACG8FK_001169"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS5096787.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="ACG8FK_001170"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="ACG8FK_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5096788.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="ACG8FK_001171"
     CDS             complement(12375..12737)
                     /locus_tag="ACG8FK_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5096789.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACG8FK_001172"
     CDS             complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACG8FK_001172"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS5096790.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACG8FK_001173"
     CDS             complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACG8FK_001173"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS5096791.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="ACG8FK_001174"
     CDS             complement(14365..15876)
                     /locus_tag="ACG8FK_001174"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5096792.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACG8FK_001175"
     CDS             complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACG8FK_001175"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS5096793.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="ACG8FK_001176"
     CDS             complement(17703..18779)
                     /locus_tag="ACG8FK_001176"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS5096794.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="ACG8FK_001177"
     CDS             18975..20720
                     /locus_tag="ACG8FK_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS5096795.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="ACG8FK_001178"
     CDS             complement(21000..21437)
                     /locus_tag="ACG8FK_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS5096796.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="ACG8FK_001179"
     CDS             complement(21421..22794)
                     /locus_tag="ACG8FK_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096797.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
BASE COUNT         6503 a         4840 c         4284 g         7226 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatctga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa agg
//
LOCUS       DAZUMV010000034        22707 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000034 DAZUMV010000000
VERSION     DAZUMV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.034"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..1140)
                     /locus_tag="ACG8FK_001180"
                     /pseudo
     CDS             complement(40..1140)
                     /locus_tag="ACG8FK_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(1232..2119)
                     /locus_tag="ACG8FK_001181"
     CDS             complement(1232..2119)
                     /locus_tag="ACG8FK_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5096798.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(2196..2741)
                     /gene="nusG"
                     /locus_tag="ACG8FK_001182"
     CDS             complement(2196..2741)
                     /gene="nusG"
                     /locus_tag="ACG8FK_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS5096799.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(2842..3012)
                     /gene="secE"
                     /locus_tag="ACG8FK_001183"
     CDS             complement(2842..3012)
                     /gene="secE"
                     /locus_tag="ACG8FK_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS5096800.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(3036..3188)
                     /gene="rpmG"
                     /locus_tag="ACG8FK_001184"
     CDS             complement(3036..3188)
                     /gene="rpmG"
                     /locus_tag="ACG8FK_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5096801.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            3350..3994
                     /gene="cbpA"
                     /locus_tag="ACG8FK_001185"
     CDS             3350..3994
                     /gene="cbpA"
                     /locus_tag="ACG8FK_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS5096802.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(4050..4553)
                     /locus_tag="ACG8FK_001186"
     CDS             complement(4050..4553)
                     /locus_tag="ACG8FK_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5096803.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(4566..4612)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5150..6115
                     /locus_tag="ACG8FK_001187"
     CDS             5150..6115
                     /locus_tag="ACG8FK_001187"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5096804.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(6214..7386)
                     /locus_tag="ACG8FK_001188"
     CDS             complement(6214..7386)
                     /locus_tag="ACG8FK_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096805.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(7373..8518)
                     /locus_tag="ACG8FK_001189"
     CDS             complement(7373..8518)
                     /locus_tag="ACG8FK_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096806.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(8532..9464)
                     /locus_tag="ACG8FK_001190"
     CDS             complement(8532..9464)
                     /locus_tag="ACG8FK_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096807.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            9581..10660
                     /locus_tag="ACG8FK_001191"
     CDS             9581..10660
                     /locus_tag="ACG8FK_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5096808.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            10657..11286
                     /locus_tag="ACG8FK_001192"
     CDS             10657..11286
                     /locus_tag="ACG8FK_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5096809.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(11396..11623)
                     /locus_tag="ACG8FK_001193"
     CDS             complement(11396..11623)
                     /locus_tag="ACG8FK_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5096810.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(11726..14314)
                     /locus_tag="ACG8FK_001194"
     CDS             complement(11726..14314)
                     /locus_tag="ACG8FK_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS5096811.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(14334..14993)
                     /locus_tag="ACG8FK_001195"
     CDS             complement(14334..14993)
                     /locus_tag="ACG8FK_001195"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5096812.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(15042..15545)
                     /gene="trmL"
                     /locus_tag="ACG8FK_001196"
     CDS             complement(15042..15545)
                     /gene="trmL"
                     /locus_tag="ACG8FK_001196"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS5096813.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(15766..16632)
                     /locus_tag="ACG8FK_001197"
     CDS             complement(15766..16632)
                     /locus_tag="ACG8FK_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5096814.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            16741..17382
                     /locus_tag="ACG8FK_001198"
     CDS             16741..17382
                     /locus_tag="ACG8FK_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS5096815.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(17444..18805)
                     /gene="mgtE"
                     /locus_tag="ACG8FK_001199"
     CDS             complement(17444..18805)
                     /gene="mgtE"
                     /locus_tag="ACG8FK_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS5096816.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(18823..19722)
                     /locus_tag="ACG8FK_001200"
     CDS             complement(18823..19722)
                     /locus_tag="ACG8FK_001200"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5096817.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(19724..20521)
                     /locus_tag="ACG8FK_001201"
     CDS             complement(19724..20521)
                     /locus_tag="ACG8FK_001201"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS5096818.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(20499..21203)
                     /locus_tag="ACG8FK_001202"
     CDS             complement(20499..21203)
                     /locus_tag="ACG8FK_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS5096819.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            21334..21909
                     /locus_tag="ACG8FK_001203"
     CDS             21334..21909
                     /locus_tag="ACG8FK_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS5096820.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            22027..22704
                     /locus_tag="ACG8FK_001204"
     CDS             22027..22704
                     /locus_tag="ACG8FK_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS5096821.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         6682 a         4849 c         4018 g         7158 t
ORIGIN      
        1 aacgaagaga ggactaattg cccttctctt cgttttttat taatccatca atttcgtatt
       61 ttttacttca tcgatcgttt ttgttcctgc aagttgcatc gtgatcgaca attctttgtt
      121 caggtgttca aaaacagatg ttactccttc tgccccacct agattcaacc cataaatcac
      181 tggtcggcca atcgcaacta agtcagcacc acttgccaga gctttgaaga catgctctcc
      241 tcgacggaca ccagagtcga aaatcactgg tacacgtttg ttgactactt cggcaatttt
      301 tggcaatact tcaaaggacg caggacctcc atctaattga cgtccaccat gattggatac
      361 ccagatacca tctgcccctg cagagatagc gattactgca tcttctggcg attgtattcc
      421 ttttacgata actggaagat gggtgatttc tttgattgtt ttgatgtcat ctggtgtcaa
      481 gccttgtttt gcagctgcat aaatttctgc gattcctttt ccttcgccat taccacttgc
      541 actttgttcg ctatacgcag ctaagttagg cattggtaat ggaaattgga attgattgat
      601 gacgtcttct tcacggtaac caccgagcgt tgaatctgcc gttaaaatga tagctttagc
      661 ccctgcttct actgctttgt tcaaaatgaa ctcattgaat ctgtcgtctt tactcatata
      721 taattggaaa aactgcggtg cgcctggcgc tgcatcggct gcgtctttga ttgtcgtatt
      781 tgcataagtg ctgatggaaa agattgatcc agcagcagct actcctttag ctgtatcagc
      841 ttcccctttt tcatgtgcca atccttgggc cgctgatggc gcttggataa tcggtgtatc
      901 taattcaatt ccgaaaacag atgtatgcaa atcggctgag tcgattccgc gtaatatccg
      961 tggcatgatt ttctttgtat taaaagaagt cgtattttct ttcatcgtcc actcatcttc
     1021 agaaccaccg cgaatatagc caaaagctcc tgcttccatg cgctctttta cacgaccttc
     1081 tagttaggca atgtttacaa tatcgattgg atgttcttca ttacttgtat gataagtcat
     1141 agtagatcct tctttcatct tcttgattac atataaatca tactaaactt acaaaaagta
     1201 agcaaacatt tactctcaga tacttttatc gctattcttt ccaaataaat cgacttacaa
     1261 gctgatctac ttcttggttt tcatgaagca tactatgaga ggcattttca cctgtcacta
     1321 ctttttcctc tatttcattt ccgcgttgct tcaacaacgc atagaccgct aatgcactgt
     1381 ttaacggtac tgttccatca ctcaagtctg tttcatctag ttgaccagca atcaagaaaa
     1441 atcgagtggt tataggaata ttaccgatca tttgctggta atcttgatag cggttagaaa
     1501 cgacagcagg cccctttgct aactcatctg tcagagactg cgcactatca tcgacaaaat
     1561 cattaaaagg cgcgccaatt gctgttaatg tctgaacttg tggcaatgac gtatcctgtc
     1621 catacgttcc taaatagcgt agtgcactaa ctcctcccat ggaatgcccc actatgttga
     1681 ctttatctat gccgtagttt tctttcagat acaaaagaca agttttgatc cattctgctt
     1741 gattccactc gttattttta ttatcttcaa acaatacttg gaccatcgga ttgtctgctt
     1801 ttccagacaa atctcctgtt gcttgtattt gtccatctgt tcctactgtt aaaaccaatt
     1861 cttttactgc ttgctgattt ttttccaaac gccgcaacat ccctttaaag gaattttctg
     1921 tcccgctgta gccgtggaaa aaaagtgttg gaatagtggt tttttctatc actttactgg
     1981 attctgccgt tttttcttta tcagaaactt cagtagtatt tatcttcgtc gtctggcaac
     2041 cacttaacgc aaacaaactg ataaggacta aagctgctgc agttgcacgt ttgcataaat
     2101 ttttgattgg ttctgtcata cacctcttcc tttccatata gaaaaaagaa agacttgaaa
     2161 ggccaatcat atgatcgaca tttcaagtct ctttattaat cgattttatc tacttgatcg
     2221 aaatcaagtt ctgtactggt ttcacgtcca aacatatcga tgttgacttt caatttttct
     2281 ttttctccgt caacttctgt tacttgtcct tctaatccag caaatgcgcc ttcgatgatt
     2341 ttcacgactt cgccaacttc tacttctaga tcattttgtc gcaggctcat accgatagaa
     2401 cgcaagatat gattgacttc ttctggaagt aatggtgctg gtttgcttcc ggctccgtgt
     2461 gaaccaacga atcctgtcac accaggtgtg ttgcggacaa cataccatga agcgtctgtc
     2521 atgatcattt caacaagtac gtatcctggg aatgttttat gaacgatttc tttttctttc
     2581 ccatttttga cttcagtttc tttttcttca ggaacaacga cacggaaaat aaagtcggcc
     2641 attttcatac tttgcgcacg agattcgata tttgctttta ctttgttttc atatcctgaa
     2701 tatgtatgca agacatacca gtttttttca aaagattcca ttgaaggctc ctcatttcat
     2761 tgtaaaataa aaaaacctca gtacacccga agtttttctt tcgcttcatt atagcactaa
     2821 aagtacgttg tggctagcga gttattttaa aatccagcca aataagcttt ggatggctgt
     2881 atccattacg taaaacatca atccgaaaat aatagaagtt tcgatgacta ctaacgtatc
     2941 tttacggagt tgttttctgc ttggccaagt tacttgtttg atttcatctt ttacgctttt
     3001 tagaaatttc atgagacaac ctcccttgct actgtttatt tcgtttcttt atgtaatgta
     3061 tattgattac aatatttaca aaacttattg atttccaaac gttcgccgct ttttccttca
     3121 ctgactgatt ttgtgtagtt gcgagaccca caaacggaac aagcgagtgc tgcttttttc
     3181 tttgccattt acttctgccc ccttgctgag aatcagactt atacttcata acagtatcac
     3241 gtaacttttt acttgtcaat ttgtgaaata aactatctat tgttttggat aaaattatga
     3301 tatgattaat ttgaaattaa gctaaaatga gacaattgga ggtatagcga tgctattaaa
     3361 aacacttgta tataagaaaa aagatctgac aaccgttaca gagactagta cattagaaga
     3421 agcattaaaa attttagaaa cttccggata ccgttgtgtg ccgatcttag attcttcagg
     3481 taatattttc cgtggaaaca tttacaaaat gcatatctat cgtcataaag caaatggcgg
     3541 tgatatgacg cttcctgtca ctcatctttt gaaaaacgct acaaagttca tttacctgga
     3601 tacttctttc ttcaaagtat tctttacgat caaagagctt ccttatattg ctgtccttga
     3661 cagtgaaaat catttttatg gtattttgac acatagtacg ttgcttaata tgctctctca
     3721 atcttggagc gtagaacaag gaagctatgt actgacaatt gcttccacag gaaagcaagg
     3781 agatttggct gctattgcaa aaatcatttc aaaacacagc agcattgcca gctgtattac
     3841 tcttgatatc gaaaaagacg aatacattcg acgtacattg atcacgctac cggctaacac
     3901 tgaagcgaaa gtatgtgaaa cgatcgtcaa ccacttagag aaaaagaatt ataaagtggt
     3961 agaaattgaa aatttgcaaa accaagaagc ataatcaaaa agcgaaaaaa gaaaacagct
     4021 aatctgcttt cttttttcgc tttttataat tagacacgct tttcaaaatc aagcgcgcga
     4081 tctaaataat acatcaatgg tgctgaaaca gccatgacga taaactcact taatgcaagc
     4141 gtgccatagg ttgtccaaaa tgcttggcca cctgttggaa ctaacatata agcaatcagt
     4201 cccatgctga tggtaaagaa gatcgtgttc aatactaaac gcatctttac atttttcact
     4261 ttgttttgca ataaagctgt caggcctaaa gaaagcaatg tttggccgcc accaaaaacc
     4321 acatccatga taccaaaacc aaagaaggca ttatagacga tgaccccgcc taaaactccc
     4381 caaagcaatt tacggttaaa gacaactaaa tgattcaaac tttctgaaat acgaaactgg
     4441 atcggccctg aggatacagg cgcaactaaa atagttaacg ctagataaag agccatgatg
     4501 atgccattta caacgattac ggataaccgt tgtctgcttt tcgttttttg cattttctct
     4561 cctcttttct cctagttttt ttaacgcggg atggttgatg aaccgcggtt atgtgaaaca
     4621 aacaataatt ataacagact tttcatttat ttcaatccgt cttatctgct tttaaaaaag
     4681 caaaagctta aacaatgtaa acgtttgcta aaaatcgtgg gatatggtat aaagtaagtg
     4741 taaggggtca taagaattcc cagtaaagca gacgaagtag gatctgcaag aacagaattg
     4801 ttgtgaaact taaataaata aaaaaggatg tgggaatttg agcaacctct tactagagaa
     4861 cggtctagca agcagctgaa gatctaagtg gacatgccga accttggaac ttctccttac
     4921 gtaagtaatg gatcatgcgc ttgctaggct tttttgtgtt tttcggtctt cggacctttt
     4981 tcattttcct agacttctgc tataataacc aagaattaat tttgtaacag atttttttga
     5041 aaaaacaagt ttctttcatt caaaaaatag aaaaagcttg ctaattattg gttaaccagc
     5101 aaacaggagt attaaaatat cttgttttat tgaatagcag gaggaaatca tgaaattcca
     5161 ttatcatata ttgatcaatc catcggcagg cagcggaaat ggctataaag tagcagaaag
     5221 aattttaccg gtattaaaaa ataagcatat tgactataca cttcattata gtgaatacaa
     5281 aggacacgat gctgagattg ctgaaacttt ggcaaaagaa acattgattc cttggataga
     5341 agaagaacag aaaacaatcg atactttccc ccttttgatc atcgtaggag gtgacggcac
     5401 cttacatcag gtacttgata ctttctatca gatggaagta gaattcccag ttgcttatat
     5461 tccagcaggt tctggaaatg attttgctcg aggggcaggc cttccaaaaa atcccaaaaa
     5521 aggacttcag ctgattttag cagctcaatc accagaaaaa gtccacataa tggcttatga
     5581 agaaaaaata agtgaaaaaa aaggaatagc tgttaataat ttcggtatag gattagatgc
     5641 agctatcgtc catgcgacaa atcattcttc cacaaaaaag cgattgaaca aatacaactt
     5701 aggatcattt tcgtatttat tctctattct ccgtgcttta ttcactcaaa aaggatttcc
     5761 aattctggta gatgcaggag gaaaacaatt aagtttttca aacgcatttt tatgtacagc
     5821 aacgaagcat cctttttttg gcggaggaat cgcgattgtt ccaactgctg atgcgtctaa
     5881 accagtcatt gattttgtcg tggtcgaacg aatcaatttc tttaaaatct tgtggctcat
     5941 tcttttactt atacagaaaa aacaattgaa atcgaaatat ttccatcact acacaacaaa
     6001 cagattacgt atcgtttcaa caacaccgca acatggtcaa gaagatggag aagaaatgga
     6061 aaaacgacct tttgatatca caataaccac aactaaacag ttgttttggt gttaataaaa
     6121 gaggttttgt cagaagctac atctggaaca ccgtttattc ggaaaaaagg actgtgacac
     6181 gacttttgtc acagtccttt ttttactatg cagttaattc tttgcccaat tgggttgcca
     6241 tcgatagttt ctttctttac ccaaaataaa ttggatcatt gaaaacaccg cagcaaaagc
     6301 aactaagaca caaccattga accaaaaaga ttctgcaaat gtttgattgt aactgagtgt
     6361 ttcactgatc aagttattat tttgaacaaa ccaatatgtt ggtgtgaatg ccgctatttt
     6421 gctaaccatt tctggcaagt attctgctgg tacaaacacc ccaccgataa agcaactgcc
     6481 tgtgataaaa atatctttga tcccaccgct ggtttctgag ttttttacca atgaagtgat
     6541 acaagccgaa aagctaatga tcggcaataa aaacaagaaa gtatttaaaa tatgatacca
     6601 taccgttata tcccacgagg tgtggatcac taacagaacc attagaagta agaagccact
     6661 gaacaacagc agcgagtaac taatcaaact aaaggttact ttgcgattca agtttcgttt
     6721 ggtaatgggc gcactctggt ttcgtttact gatctctttg cgattaaacg ctagattgat
     6781 gacgctgaaa ccgctaaaaa tggatagaaa caacccataa gacaagaaat taaaggttgt
     6841 gcccgtcaac agctttttca tccgctgact atatcccgca cttagctcaa cttctccagt
     6901 aaccgagagt gccatttgtg tctgttccat agctgcatcg attgttttat cttcttcttg
     6961 aaacagaatc agtgtttgca aaaaggtgtt cacttgctgt gtaacaactg ttttagaaaa
     7021 ggcatctggg cgactctgta tcttgatcgt cgccatcttt cctacttcga cttgttcggt
     7081 aaagtctgca ggcagatgta agataatgtt tacacgatta aaataaagag catcatcgat
     7141 ttctttttgt gaggttccat ccaatgtaac gactgtttcc ttgtcattca aatatttacg
     7201 aaggccatcg actacaggcg actcttcttc actaatcaag gcgattttcg catcttgcag
     7261 agaggtattt tcaacatcaa tattttgcgc aaaaaagaat gtggcaacaa cagtgataat
     7321 tacacctatc agtaaattcg atttatttgc tttaagcaat gtccaaatcg ttttataaag
     7381 cgtcataacg cacccctctt tccatccaag catttcctaa cgcaaaaagg aaaataaatc
     7441 cgatcaacca tagaagattg gtataaaaac tagccaaatc agtgtagaaa taaagtttat
     7501 acaaactttc gctgatcaag ttgacgagat ttaattgacc aacaatcgga atatgaagat
     7561 caatccaata cttcacttgt tccgacccca tcataccagc aaaaaagctc atcgcaatcg
     7621 tcactgaaat caagatgcta tccttttgca ccagattcat ttttggtatc aaattaccaa
     7681 ccaatgtacc taacaaaatc gcacacaacg accccagagc ggtaacaatc atcaaccatt
     7741 gccaacgttc accaaagtct acttgataga taaaacgaaa gacagctaac ataatataaa
     7801 tctctgtcaa aacaatcgta aagcttgctg ccaaattagc gattgaaact aacgattgct
     7861 ttctaggaat caaggacaag cgaataccat ttgacgattg atttgcttgc tgatcctgta
     7921 cattgcggac cccccacatc gtcccgtaga gaatcgccat tccaatcaga gtgaagaaat
     7981 aaaaactttt gaaactcata ttacgacctg catgaacttc ttgaaaagat acttcttgtt
     8041 caaaaacagt catgatgggc gcgagatcaa cgttctccac ttctgccact ttactcgaaa
     8101 ttataccaat acgctgtaaa aattgatcca tgacgttttt catcaacgtc tgactcattc
     8161 ctgcttgacc cacatggagc gaaacagatg aaccatcaat gagataaaaa ccagccaaat
     8221 catctgcatt tagcttttca ttcgcttcta cttccgatag ttcctgtact tcaaataatg
     8281 gtttatcatt gacttcgact tgttctaaga ctgtggtaaa attttccgaa agcgctgttt
     8341 gatccgcttt gattacaatt ccagtattaa tcgtttcaag attgccacct tgatcaatct
     8401 cgccaaaggc cgccataaac atcaatccta aaagaattgg aaaggccaat gtccaaaaca
     8461 acatggattt atttcgcagc agtatttttg tgcgatattt ataaaaatgc caaaacatta
     8521 tttcccctcc tctagtctct caaatccttg ccagtaatct ctaaaaagac atcattcaag
     8581 gacgcttctt tggttgctat ttttccgtaa ctgatctttt cttcttgtaa gattgtaaga
     8641 atcggcaaca atactgtatt tagttcagta gttgttactt ggagctgacc ttttttataa
     8701 gcgattccat cagaagctat tgttcgctga atcgctgaca acacattttc tgggagtaat
     8761 gggatgtcga tcgtcaaacg atccgccgtc cgtaccatat cttttagaga ttctttggtt
     8821 cctttagcaa tcacctgtcc ttgatccatg atcacgatct ggtcgcaaag aatttctact
     8881 tcttccatgt aatgtgttgt atagacaatg gttgctcctt gtcgattcag ctccttgata
     8941 ctttctaaaa ttttattccg actttggggg tctacagcta ctgtcggctc atctaaaaaa
     9001 attaaccgcg gcttgtgggc aatcccacat gcgatgttca accgacgttt caagccacca
     9061 ctaagttctc ctggacgaaa cgaaacatat ttttctaatc ccactaattg aatcacctct
     9121 tcaatcaagg ctttcctttt ctctttttcg gaaacatata aaccgcaaaa ataatcgata
     9181 ttttcttgca cacttagttc gtcaaagacc gcaacgtctt gaggaacgac cccaatcatt
     9241 gccttgacag cataattttc aggagtcatt gttgtcccga acaatttaat ggttcctttg
     9301 tcataattta acaaagaaag catacagttg atggcagtcg atttcccact gccgtttgga
     9361 cctaacagac ctaagacttc tccttcttgt acttgcagat caaaatgatt caatgctaca
     9421 aaatctccat agcgtttgac taatttatca atttcgataa tcatcatgaa aaactccttc
     9481 caaatttctt gttgatctga ctataataaa gtgcaagaaa agaaactagt gacgattggc
     9541 taaaccacac atgacatttg tcacaatgaa ggagtctgct atgcataaat ctcggctact
     9601 caacgaaact agctttctac tggttatctc attgcttttg ctggtaagtc aaggattcgt
     9661 tgctatgcaa gttatctatt tattgatcgc cgttttattc actggtgtca gttatttact
     9721 gcctacaaag ccaaattgga ttttccaagt tattctattt cttttatgtg cctttttacc
     9781 tgatcttgtc ctgtttctgc cagcgacgat gcgagtgatc gcccctgaag aaagccacct
     9841 tgattacttg tttttgttag gttttttgtt gatttcgacg gttggtaatc atccccccat
     9901 caaggtcgcc tttgtcgtaa ttttgctgtt tttttccttt tacttacgaa aaaaagatca
     9961 acgaacgacc gatcttgaga aaaagtacat tgtctccaaa gatactcatt gggaaaaaca
    10021 acaagagtta tcggaacaaa ataaagcatt ggccctgtca caagaaacac aattagcctt
    10081 gcaaattgtg gaagaacgga accgaattgc ccgagacatc catgacaatg tcggtcatct
    10141 gctgtcaagt gcgattttac aaattggtgc gcttgaaatg atcaatcaag aaccaaaact
    10201 aaaagaacca ttagaaaatt taagtgccac cgttcacact gggatggatc ggattcgtca
    10261 aagtgtccat gacctccatg agacatcgat ctcttttcaa cactccttag agtttctgat
    10321 cgctgatttt ccttcttctg ttacgattga aggaaacttg tttgaaggat tgaatgaaac
    10381 gcagcaaaac tgcttcttaa tggtcatcaa agaagcctta accaacagta tcaaacatag
    10441 tcatgccaaa accgttgttt tacattttcg aacgttgccg gccttttatc gcttgcaaat
    10501 cacgaatgat ggcaccagcc cacaaaaaaa ttctgctccc ggtattggtt tgaaaacgat
    10561 gcgtcaacgt gttcaagaac aaaagggaca agtccatatt ttccaagaaa accagcaatt
    10621 tcagttaacg atcattttac caagggaggg acaccaatga taacagtagt cattattgat
    10681 gatgatccat ttgtgacgaa atcgttacaa accattttag aaagcacgaa agagatccgt
    10741 gtgttaggca ttggtcattg tgccaaggat gcgctagtct tatacgaaac ccatcgtccc
    10801 gatgttttgc tgacggacat ccgcatgcct aaacaaacgg gcatcgatgc tgccaaagaa
    10861 attttggcta aatttcccaa agccatcctt ttgcttttga cgacattcaa agatgaagaa
    10921 tacatccgcg aagcattttc catcggtgtc aaagggtatt taatcaaaca gaacttgcaa
    10981 gcgattattc cttctgtcaa atccgcttat aatggacagg ccgtatttgg taatgaaatc
    11041 atcgagactt tcactcaaat gatgaagaat aaaccgacga ttgatagtca tgcttatgct
    11101 tcttttagtg aacgtgagct tgcaatcatc aaagaagtag ctgccgggaa aaacaataaa
    11161 gaaattgcag atgccttgta cttgagtgac ggcactgttc gcaattacat cagccaatta
    11221 ctagaaaagc tagatttacg ggatcgcacg caattggcta tctattttta tcagcatttg
    11281 caataagtct tatctaacac aaaaaaacct gaatgatgct ccttcgcatc attcaggttt
    11341 ttttcacatc ttctattcaa ttgtcccgca aaccatttcc tcacgattga aagaatcaag
    11401 gattcgcagg gctgccagga ttcgttggat tgttcggcgt gttaggcgga aaaataggcg
    11461 ccttgtctaa cgttttttct agttttgatt cttcacgatc gtctcgttct acatcaggac
    11521 tttcattcaa tttgaattct ttttccatct catctaactt tttaaagtca ttgccagatt
    11581 cttttgctaa tttcgctaat ttatcttcgg tttctttagc cataaagtaa cgcccccctt
    11641 ttgatttcag cttagcatat cacactgaaa aaacaaaaga aaacgctaac caacgatcat
    11701 ttttagctat ttctcatctt taaaatcaag cattttttgc cggtataaaa tcgtagggag
    11761 aactgttttc cataccaatc atcggatcta ctaactggtt atcgatcact tctgctgtaa
    11821 gaacgaaatc tttagaaaca gcaggttcta cctcttcttt cttttcttct ccaacaaatg
    11881 aagaaaacag atctggttcc actgtttcca ttacttggtt tttcgtttca ttcgtcccac
    11941 tgtcatcttc tacaggtagc ttttctttta caacccgtgg ctcttttgta tagttcccat
    12001 cttcaaatgg atcatttccg accaaagaat cttcataagc tgctagtttg gtccggagag
    12061 tgatattcat tttatcatta tccatgatcc ttgatttcaa cgtagtcatc cggcttgctg
    12121 attcattcgc tacacaagtg acataggctt gctcttcccc taacaaaatc aacagttcct
    12181 tactgcgagt cactgccgtg tagagcaaat tacgctgaag catccgctga tattgatgga
    12241 ccatgggcaa aataaccatc ttgaattcac tgccttgtga tttatggatt gagcaacaat
    12301 aagaaagagt gattttattc cattcgtttc ttttataagt gacctcattt gcatcaaact
    12361 ggataactaa ttcatccact ttatcttcag aatcttttgc caagatgata cccgtgattt
    12421 ctcccatatc tccattgaat acattcaatt cgggagtatt gactaattgg aggactttgt
    12481 ctcctatccg ataaacggtg tcgttccaat gtacttcctt ttttccaggt ttccctggat
    12541 tgaatatctc ctgcatcatt ttatttaacg catcgatccc tgcaggtccc cgatacattg
    12601 gtgctaatac ttggatatct tgtgcacgga atcccttatt tttcgctttt tccacaattt
    12661 tagagatcaa gggttcaatt tgataagcat cacttcggaa ataggaacga tctttttgat
    12721 tctttgtgaa atcttttggc agacgtcctt ctttgatctc atgggcaaga ggaataatac
    12781 tagaaccatc accttgccga taaatctgat tgagttccat tttgggaatt tgttcgattt
    12841 ccaaaagatc atggagtact tgaccaggac cgacagaggg aagctgatct ttgtctccta
    12901 ctaagataac ttgcatattc gtcggaatcg ctttgaataa tgtgttagcc agccatgtat
    12961 cgaccataga catctcatca acaatcaaaa gtccgccttc taattctttc gtactgactg
    13021 aaggtgtttt ttcccgtcca ttcagcccca gaagacgatg gatcgtactg gcaggcagac
    13081 ctgtcgtttc gttcattcgt ttagctgctc ggccagttgg tgctgcaagt agaataggaa
    13141 aaatttcttg ggtatatttt gaaggatcca attccagtcc gttcaactct gcaaaaagat
    13201 tgacgattcc gttgatcaca gtagttttcc ccgtacctgg tcctcctgtt aaaataaata
    13261 gaggggaacg gattgcttca gcgatagctt gttcttgcga aggtccatac tgtatgccga
    13321 atattttttc gatacgccga atattcttct gcacttcttt ttcaggataa tggatttctt
    13381 ttttacgact gagaaggcgc tggatcgaat taccgatccc ccattcagaa aagtacaaac
    13441 tgttttcgta aagattagtc tcttcttgtt gtatctttcc ttcttcaaca agttggataa
    13501 ttacagtagc aacctgatca ggctggattt caattgggcg acttgtttct aaaatacgga
    13561 tcgtttgctg aagcaatgtc tccgccggta cgtatgtatc tcctgtttga actgcatgct
    13621 gaaaaatctg atgcaagata gctgctcgaa ttcttctcgg tgaatctgct gcaataccga
    13681 tttgttcggc tagattatct gcacgtttga acccgattcc ttctatgtct tcgactagct
    13741 ggtacggatt ttcttcgatg atatccaaag actcgttctt ataggcctgg tagatactga
    13801 aagctaactg actaccgaac ccgtaacgat tcagtcccat gatcacttga tccatcccgt
    13861 ggtttagccg aatcgtctca gcaagcattt ctctttttgg cttcgtcaaa ccggagattt
    13921 gttttaatac cgaaggatca gcaatgattt tttcgatagc atcttctccc agcaattcta
    13981 caattttttc tgctgttttc ttcccgattc ctgggaattt ttcgctggaa agatacgcta
    14041 cgatcccatt tgcagttgtt ggcttttctt gctcatatct ttcgagttta agctgccttc
    14101 cgtatttagg atgatccact aattctccat aaaaacgata agtctcattt tcttgaatat
    14161 cgccgaaact tccggtgact acaatctctt tttcacggta atcagctgtg gtttcactga
    14221 tgtgtaccaa aagcactttg taaaaattac taggattttg aaaaaaaatc gcggcaactg
    14281 taccaacgaa ataaggcatt tcttctggct gcatcggaag ccgcctcctt cttctatagt
    14341 aaaggttctg gtgctgtttc agttcctaaa aaatctgttt ggttccatac tttcagttca
    14401 tatggcaata atctgcaagg ctcttcagtc tctaaaatcg tcaaactact attgaacaat
    14461 ccacccattt cacgcaactc acttagctct tttcctgtca tccattgaat ggctgcagtt
    14521 aaagaagctc catgtcctac gaataaaagt gggccatctt tatgtcgggc aaccgcatct
    14581 gctacaacag tttcgatacg tgtgattgcc gattcaatcg tttctccgtg aaaaggagaa
    14641 ggatcataca gatctaaatg attacgcaaa ttcggcagat tatccggata tttctggtac
    14701 atttcatcga tcgactgacc ttctaaagta cccagcccta gttctcttag cttatctgta
    14761 taaaaaatct ctaccggacg agcaagctct ttgttgatac cttctgctgt atcacgtgct
    14821 cgctttgctg tactggaata aattgcttta aaaggaacat attttacctt tttcccaaat
    14881 gctttgattt cttcgtaact ttgtggcaaa agcggagaat cgccattgct cccttgaaaa
    14941 cgtctttcct gattccattg tgtcttccca tgtcgggtaa aatatagttc catacgcagc
    15001 aaactcctgt ctttctttta ttttactcta taataaactg tctaatccag cttgtcattt
    15061 tcatagtgat gcgtcaattc taaattaggg aatccttgct ggcgaagtgc ttcatacact
    15121 atcattgcag ctgtattcga taaattcaat gagcggacat gctcatcatt catagggatg
    15181 cgtaaacact tttcttcgtt ttcacgcata aatgtttctg gcaaaccggt tgtttctttt
    15241 ccaaataaaa agaagtgctc ttctccatca gcaaaatttt cgtcactata tactttatgg
    15301 gcaaattttg agatcaaatg tagtttacga ttacctaaaa atgttaaaaa agcttctaaa
    15361 ttactgtggt aagtaatatt tacatcattc caataatcca accctgcccg ttttagatgt
    15421 ttgtcatcag ttgaaaaacc tagtggctcg attaaatgaa gcggagtatt cgtggctgca
    15481 cacgtacggg caatatttcc agtgtttgca gggattaatg gttcaaataa aacgatatga
    15541 ttcatcagga tcattcctct catatttatg ttcgtttcta ttttctgaca atttgatttt
    15601 tgcataaaaa aaaacgtatc aactcggtgt cagtcactgc gagtcgttac gttagtgaag
    15661 ctcttctcac taacttttat cagaacagga acctgataaa aaccaatgaa aaaacaactt
    15721 gttgctaata taacaccctc aacttttttt tgcaacctct actcattaaa aactgctaga
    15781 aaaacgtttt ctcttagttc cttttaataa ttctatgtca attgtaatct tcttcaaagg
    15841 attcttatat aagtctgctt tgattcgctc ttctacctgc cattctaaag gtgacatggc
    15901 caaaagatct ttttgatttt cctctggtat ctcaaattca taggttacac gctgacgttc
    15961 agttaatttc attgcttcag caaacttggc tactacacgt tcattggaat aactctgttt
    16021 ttcaggttga ttcggataaa aagcctgacg tagttccttt aaataatttt cttgtggcac
    16081 caccttcaac acttctccat cttctgagag tacacgttgg aattcttggt aatgagaagg
    16141 cgaaaagata tttaaaaccg cgttgaatgt ttgatcagca aacggcaaat tggtcaaatc
    16201 agctacacac caaaatgctt cggcagattg ttctgtagct gcgtagactc cttcttttga
    16261 aatatcaaat ccaacagcat tttgaatctt tttcaatttt gaaagttcat tcaagaagct
    16321 accttctccg cagccaacat ctaatagcga gacttcttca ggtaaatgct gcgcaatagt
    16381 ctcaataacg ggctgataca tgccagattg gatcattcta cgccttggaa cgaacattcc
    16441 ttgatcgtat tctgttttaa ccgcatggga taaaaaataa agtgttcctt ttttggaaag
    16501 gtcaaaacgg tgattatttt tgcatatcaa gccattatca gactttttta cttcttcttg
    16561 gcaaaccgga cagcggaaca gctgatgatt ttctgccaaa aactggcgtc ctcggtcaat
    16621 tttttttaac atagttcccc ctcatttctg ttttatctta ccgttagatg ctcactttgg
    16681 caactgctaa aaatatgata caatgcctag aagaaagtga aagataaagg agaagattag
    16741 atgatcaaag aattttgcgc tgaaaactac acatctattc ctctagcaat tgctagagga
    16801 gccgatcgga tcgaattgtg tgacaactta gcggtcggtg gcacaacccc gagtactggt
    16861 gtgatcgaag aaacgttaag ttatgctggg gaaaaagata tccccgtaat gacgattatt
    16921 cgtcctagag ggggcgactt cgtctataac gatatcgaat tgaaaatcat gcatactgat
    16981 ttgattgaag caaagaaatt agggacggat ggcgtagtat ttggttgtct tactccttca
    17041 ggctggctag acgaagaggc cttagaacta ttgatcgaaa cagcagaagg gttgcagatc
    17101 accttccata tggcttttga cagtattcct gaagatcggc aattcgaagc aatcgattgg
    17161 ctggtagaac atggtgttga ccgtatcttg actcatggcg gtcctgctga caaaactatt
    17221 gtagaaaatt tcccacggct taaagaattg attgcctata cagatggccg cttaatcatt
    17281 ttgcctggtg gtggtgtcac tgcagagaat gcagcacatg tagctaaaga attgaacgta
    17341 agcgaagtac acggaaccaa aatcgtggat cttcagttat aatcttataa acaaaaaaac
    17401 atttgcaaca ggcataatgc ttattgcaaa tgtttttgtt tttttacata aaatagccca
    17461 taaataaaga agcaatattg aaataaatca gtacactcgt caaatcgctc aaagtcgtga
    17521 taaagggacc gcttgctact gctggatcaa atcctagacg atccattagc atcgggatta
    17581 aactacctgc aagattcgcc actgtgattg caaatagcat agccatccct atgacaaacc
    17641 ccaagataaa attatgtttc caaaaaccta cgattagaaa aatcgtgatc cccgtaacag
    17701 ctcctgtgac aacgccggtc aagacttcag ccaaaacaag tcgaacaaat ccatgatctt
    17761 tctcatcaga aatcgcaagt cgacgaacag ccacagctag cgactgggtt ccagcattac
    17821 cggcagttcc tgtaatcagc gaaatgaaga ctgctaaaat actcgcttca cttaccaagt
    17881 cttcatagtg actgattaaa gaagcggtcg acatacctaa aaacaacaga gtgatcagcc
    17941 aaggcaaacg tcgagacgct gcctttactg gattttcatt aatttcttcc acattgaccc
    18001 cggcaagtcc tgaataatcg ctagcagctt catcatcaat aacgtcgatg atatcatcga
    18061 ctgtgacgat tcctaacaaa tgatcatcat aatcggtaac aggaagagct aagaagtcat
    18121 aatctctaaa tgtttgtgcc acatcttctt ggtcgtcgcc tacatgaact gaaagcactc
    18181 gttcgctcat cagatcagaa atcattgcgt cgtcatcatt cacgattagg tcgcgcagag
    18241 aaataacccc tactagctga ccttcttgat taacgacata gatgtaatag attgtttcag
    18301 ctacatctgc ttcgttcttc agcacgtaca tcgcagagcg aacagtttga ttggctacga
    18361 tcgaaacaaa ttcagttgtc atgatcgcgc cagccgtttc atcctcgtaa tggagaagtt
    18421 ccttgatttc actggcatcg tctgccgaca tcaaacttaa atatttagca atttgctttt
    18481 tatccagcgt attaagcaag tcaacagcat tatctgtata catctcagaa agcatatcag
    18541 ctgcatagct aggacgcatt tcatctaggt attctttcat atgttcgttg tcttcttcaa
    18601 tcacatcgaa catatccgct aattctttag gcgacagata ggaatagatc agctgcctgt
    18661 tctcttctgt aagagattgg tagaattgcc cctgctcata gatatgcatc tctaaaaaac
    18721 tgtttctgaa tgcctgaata ttattttccc ttaaatcttg agtcaactgg gcaaatcttt
    18781 cctctaattc ttgattttca tccaaaccaa tctcacctcg ccttattctt caaaccgtct
    18841 cacaactgcc tccatgtctg cggctaaagg ctgcttgaac tccagcaact cttctgtaaa
    18901 tggatggtaa aaacgcagat cataacaatg aagggcttgt ctttttattc ccagatccat
    18961 tcgcccgcca tacatatcat caccgagcag ggcacagcca attgcggaaa aatgaactct
    19021 tatttgatgt gtccttcctg tatgcagctg gatatcaact aacgcgattt ctgttagtcg
    19081 cttctctatc cagtactcag tctgtgccga acgtccttca tcagagacca ttcgcttcaa
    19141 tagcgaagaa taatctcggg cgatcggcaa atcgatatgt ccatgtgccg ataaatctga
    19201 aagccttcca ctgactaagg cctgatattt tttcacaaac ttcttttcac gaagttgcat
    19261 atctaatttt gcgtgcgcaa atccatgctt tgcaaaaagc atcaagccag atgtatcccg
    19321 atctagtcgc gtcaccacat ggattacttg gttttcgtaa ccttgttttt tataataagc
    19381 ctttacacga ttagccattg tcccatttgg atggtattgg gcaggaatag acgaaacgcc
    19441 tgctggttta tttactacca gaacgtgttc atcttcatac acgatatcga tcggtgtttc
    19501 atccaaaagt acggtttcat gttctccttc agcaggaatc acaatggtta ccttatcatc
    19561 cttagctaga gaaaacaaga cattttctac ttgatcatta actttgattt gaccgccttg
    19621 aaattttatt ttcgctaaca gaccctttga gatccctttt tcttttaaaa aatattttac
    19681 ttgctgagaa tgatctttct cataatccca tgtaaactgc atatcaatcc tcactaataa
    19741 aggcatcttt cacacgatgc caaaaatgca tatgtcgata agaagcaaaa tggatccgtt
    19801 cttctgctat tcgataataa attgagcgga tatttgcttt tgcgacattc aattgatcaa
    19861 ccgttacaag ataatcttct gtattttcca attttatttc tagccattca tcatgggcaa
    19921 tcacgatcgg agaacccaaa gtacgaaaca ctcgattatt cagtgaagca atctctgcca
    19981 actgaaaggc attgatacta ggatggatca cagcaccgcc gacactttta ttatatgctg
    20041 tcgaaccagt aggcgtagaa atcgtcaatc catctcctct aaaactttca aacagctcgt
    20101 ttttgatgta tacatcggct accatcgtac gattggcccg tttaatcgtt gattcattca
    20161 gtgctaaaaa atgctggtcc ggtgtttcat ccaaataact gatccgaaca tccagcaagg
    20221 gataactaac actttgttcc cggttcgttc gtaagctgtc tactagttct tccagctcgt
    20281 aatcccgcca atccgtgtaa aaccctaaat gacctgtatg cacgcccaag aaacgcacat
    20341 cattcagaca atgactgtag tgatggaacg ccgacaataa tgtgccgtct ccgccgactg
    20401 agatgaccaa ttctggctca tgatcatctc gttgaatccc tgcttgttct aaaagtgcgt
    20461 tcaaacgttc tgtcacttct atagtctttg cttctttatt atgaacgatc gctactttca
    20521 tcccctgcct cctctctttc gctttctttc ttcaaaagcg ttcgatagta gacatcatct
    20581 gattttcctc ttccatggga gaataaccgc tgtgcttctt ggatttcttc acggatactg
    20641 gacatctctt catccaactg ataggcagct tctgccgcac gctgaagtcg ttcactcatt
    20701 tcttctggaa aaacgccttg atatttataa ttcagcgaat gctcaatcgt tgcccagaaa
    20761 ttcatcgcaa gagtccgaat ctggatttcc gccaaaattt tcttctcacc gctgatcagc
    20821 tgtacaggat actcgatcac cagatgataa gaacggtagc cacttgcttt tttatttgtg
    20881 atgtaatctc gttcttggag aactttcatg tcttttcttt tacggatgat ctcaacaact
    20941 tgatggatat cttctacaaa ctgacacatg atccttaacc cggcaatatc agacatttct
    21001 tcttctaaac gtcccattgg aatatgccgt aattgtgatt ttgttacaat actatcgact
    21061 ggcttgactc tcccagtgac aaattcaatc gggacatgtc gattttgttc acggaactgt
    21121 ttgcggatcc cccgcagttt cactttcaat tctgatacag tttgttcata tggtgctaaa
    21181 aatgcttccc aatctcgttc catagctgat acctctactt cattaacatt cattgtttat
    21241 tctaccatat atgcaaagtt ttttcgtact cttgatttat gcattgacca aaaaaagaaa
    21301 attaggcaaa atagacaaaa aggagtcgat caaatgacac aaatgcttga aatcgaatat
    21361 aaatctatgc tgacaaagca agagtacgag atattcatcg ctcactatca attaacagat
    21421 aaagatttcc gtgtacagac taatatttat ttcgataccg ttgatgaaca gctgaagaaa
    21481 caaaattgtg gcctacggat ccgtttcgtt ggtcatcaag ctgaatatac attaaaaaca
    21541 cctgctgaaa aagggcgtct cgaaacaacg gataccttta taacagaaga agcagaagca
    21601 tttatcttcg aaaaacgtct gcctcgttca ggcgcagttt ttcaaaaact ttctgatttg
    21661 aatatcgatc ctgcttcatt aatccaaacc ggaaagctta caacaaaacg agctgaattc
    21721 cctattgaag aaggactttt agctattgat gaaagctggg gagacaatct acacgatttt
    21781 gaactggaac tagaggtagg ggatgcatct gtgggaaaaa ttgcttttat aaatttttta
    21841 aaacgttttt ctttacctta tcgtcctgct aagaacaaga tccaacgtat gcttgaagct
    21901 aaaaattaga aacctttcat tttcacaagg cacttgagtt cattttttta cttttttctg
    21961 ttaagttata ggatgtgaaa gaatctagtc actagttgta tagaatctat tttgggggag
    22021 attcatatga tcgaaatcta tttatttgtt aatcctttag gggaatactg tttcgaaatg
    22081 gaacaacagt tattacaatt tatcgaagaa gagtacggga caccatcaaa agaaaagatg
    22141 caatttcgct ttcttccttt ggtcaatttg cagacaattg gggatgtcat gcaacaaaaa
    22201 ggaatttctc agaacaatct tgaggaacgt aatcacttat tctcaatgac ttattctgca
    22261 gcattggact gcaaagcggc gcagtttcaa ggaaagaaaa aaggccgccg ttttttgatc
    22321 aaattgcaag aagcagtcgg ctgtaatgac ataccatatt ctcaagaact tgctgaatcc
    22381 atatttgaag aaatcggcgg cgatttgaat atgtttaaag aagatcgtca gtctgacttc
    22441 gttaaagaag tattcttttc tgatcaaaaa attgctcggg aaatgggcat cacaaaacac
    22501 ccttctgcag tcgtttacaa ttatgcctgc gaccgtgatt ttggtgtttt agtagaagta
    22561 aaaggtacaa gtagcctttg gagaatcaca gagctgtgta aaacaacaga taactcatat
    22621 caagttttta atggtgaaca tttacataca aatgattctc agttccattc aagaactagc
    22681 catttacact tgctttctaa ttaaaaa
//
LOCUS       DAZUMV010000035        22263 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000035 DAZUMV010000000
VERSION     DAZUMV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.035"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..897
                     /locus_tag="ACG8FK_001205"
     CDS             <1..897
                     /locus_tag="ACG8FK_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS5096822.1"
                     /translation="FLLKICKENFPATSVKDVQKEFTEYEYIDFFIDLSVDILRPNLP
                     ICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTVFDLF
                     IPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDF
                     REQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIE
                     QIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISE
                     YIFELKEEALMDQSKKENQVTS"
     gene            complement(1005..2087)
                     /gene="uxuA"
                     /locus_tag="ACG8FK_001206"
     CDS             complement(1005..2087)
                     /gene="uxuA"
                     /locus_tag="ACG8FK_001206"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5096823.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(2078..3724)
                     /locus_tag="ACG8FK_001207"
     CDS             complement(2078..3724)
                     /locus_tag="ACG8FK_001207"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5096824.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            3908..4570
                     /locus_tag="ACG8FK_001208"
     CDS             3908..4570
                     /locus_tag="ACG8FK_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5096825.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4714..5277
                     /gene="ahpC"
                     /locus_tag="ACG8FK_001209"
     CDS             4714..5277
                     /gene="ahpC"
                     /locus_tag="ACG8FK_001209"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS5096826.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            5287..6816
                     /gene="ahpF"
                     /locus_tag="ACG8FK_001210"
     CDS             5287..6816
                     /gene="ahpF"
                     /locus_tag="ACG8FK_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS5096827.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            6973..7323
                     /locus_tag="ACG8FK_001211"
     CDS             6973..7323
                     /locus_tag="ACG8FK_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS5096828.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            7422..7952
                     /locus_tag="ACG8FK_001212"
     CDS             7422..7952
                     /locus_tag="ACG8FK_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5096829.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            8106..9257
                     /locus_tag="ACG8FK_001213"
     CDS             8106..9257
                     /locus_tag="ACG8FK_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5096830.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(9366..11405)
                     /locus_tag="ACG8FK_001214"
     CDS             complement(9366..11405)
                     /locus_tag="ACG8FK_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5096831.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQMIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(11463..12020)
                     /locus_tag="ACG8FK_001215"
                     /pseudo
     CDS             complement(11463..12020)
                     /locus_tag="ACG8FK_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     regulatory      complement(12168..12334)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(12559..12765)
                     /locus_tag="ACG8FK_001216"
     CDS             complement(12559..12765)
                     /locus_tag="ACG8FK_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096832.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(12781..13395)
                     /locus_tag="ACG8FK_001217"
     CDS             complement(12781..13395)
                     /locus_tag="ACG8FK_001217"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS5096833.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(13542..14225)
                     /locus_tag="ACG8FK_001218"
     CDS             complement(13542..14225)
                     /locus_tag="ACG8FK_001218"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5096834.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(14334..14792)
                     /locus_tag="ACG8FK_001219"
     CDS             complement(14334..14792)
                     /locus_tag="ACG8FK_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS5096835.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(14876..16639)
                     /locus_tag="ACG8FK_001220"
     CDS             complement(14876..16639)
                     /locus_tag="ACG8FK_001220"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS5096836.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(16860..18320)
                     /locus_tag="ACG8FK_001221"
     CDS             complement(16860..18320)
                     /locus_tag="ACG8FK_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096837.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(18528..19454)
                     /gene="glsA"
                     /locus_tag="ACG8FK_001222"
     CDS             complement(18528..19454)
                     /gene="glsA"
                     /locus_tag="ACG8FK_001222"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS5096838.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(19477..20904)
                     /locus_tag="ACG8FK_001223"
     CDS             complement(19477..20904)
                     /locus_tag="ACG8FK_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5096839.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(20935..22149)
                     /locus_tag="ACG8FK_001224"
     CDS             complement(20935..22149)
                     /locus_tag="ACG8FK_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS5096840.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         6575 a         4371 c         3922 g         7395 t
ORIGIN      
        1 tttctattaa aaatatgtaa agagaatttt cctgcaactt ctgtgaaaga cgtacaaaaa
       61 gaattcacag agtatgaata tattgacttt tttattgatc tctctgtaga tattttgcga
      121 cctaatctgc caatttgtcg cgaacaacgc atcttagtga atattttagc tcgactttct
      181 atcacgaaaa tcgaatcgga agaaacaagc ttggagcaat acaaactttt aaaaaaagca
      241 aacgttcctc atgttcttta ttcaagagaa atggtggagt ccctttgtac tgtatttgat
      301 ttgtttatac ctgaaaaaga aaagattctt tactcattgg cattcgcacg aaataaactt
      361 tataatggat ttcttaactt caatcaacca aatacgcccc ttcctacttt catgctttat
      421 caagaacata gcgtattgaa agaagtccaa accgccattg aacaatttta tatggatttc
      481 agagaacaaa atcaacactt gttaccagtt attttagaaa aggaaaatgt gcaatggatg
      541 gttgaagacc tagtacattt gtatgatcgc tacaaaaaac agccgcgaat tgtgattggt
      601 gtgaactata cgcgtgattt ttatatcagt aaagatctga tcgttaagat agaacaaatt
      661 ttttcaaatg aatcgatcaa aatacaaaat gattttatgg agaactgcaa catcgttatc
      721 agcgactgcc ctttgaaaca attgccttct catatcaaaa aaatctatat tttaaaagga
      781 atcatcacac cagaagactg gaaaaaagtg attatgagga tctcagaata tatctttgaa
      841 ttaaaagaag aagcactcat ggatcaatcg aaaaaagaaa atcaagttac ttcctagacc
      901 aaataagata cataaatccg aatgcttatt aacaacctcc ggatttatgt atcaagaatt
      961 tgaacatcct tcttatttac ataataaaat tacagttttt ttcactaatt atctttactt
     1021 ttgtttcctt tttcgattgc ttcccataat ccatttagat aaacagcacc taatgctcga
     1081 tcatataagc cgtatcctgg tcttcctgtc tcattccaga tcattcttcc atgatcagga
     1141 cggatgtatc cgttaaatcc gttgtcatac attgttttta gtatgcggta catgtctaat
     1201 gaaccgtatt cggatttatg tggtgcctca tagaaatctc gattagctaa aaatttaata
     1261 tttcgaacat gtgcaaatgg gattcgatct ctttggcaaa attctgctaa aatcccatat
     1321 acatcatttt ctggatcttc tgcaatactc cctgtacaaa gagtgattcc gttataaggg
     1381 ctgtctacta tgcgacacaa ccaatctaaa tcttctcgat tctttacgat tcgagctaat
     1441 ccaaaaatcg agtatggcgg atcatctgga tgtacagcca ttttgacccc tacctcttca
     1501 catgttggga tgattgattg gagaaaatag gcaaaatttt ctcgaagttt ttcttcgtct
     1561 actgatgcat aggcttcaag caaggatttg ataggagcta agcgctcagg ttcccatccc
     1621 ggcaatccaa attcattgct ggaatgttcg atgcgcgcga cgatttctct tggatcatct
     1681 ggaatatcat cagatataaa agctaacgtg gatgagccgt cttctaagcg gtagtcaagg
     1741 tcagatttca cccagtcaaa tacaggcata aaattatagc agatgacttc tattccatat
     1801 tctgccaagt ttcggattgt ctgcttatag ttttcgatat actgctcacg ggaaggtaat
     1861 ccaattttaa tggcatcatg cacattgacg ctttcaatta cttttagtgt taacccacga
     1921 gactcaattt cttcttttag ttctttgatc ctctccctag gccatacctc tcctactggt
     1981 atatccatca atgtgcctac aatccctttg accgcaggaa tctgtctgat ttcatctaag
     2041 gtaatctttt cttcctgtga tccgtaccat ctaaaagtca tttccatgat aaatttcctc
     2101 catgttcttg taagtaatcc tgtaatgttt ttcgtacggc atttggtcct gctaccatct
     2161 tgatgaacat ttcttctatt tttctgccaa gctctgcttg gtaaagatct acaccaaaaa
     2221 tttctttatt tgacaaaata ggagccattt tctcatgaat atgtgatgta tcttgcgtac
     2281 ccagttgaat atcggaaagc ttatgctgaa ggtctgtcaa tagtgggtct ggactcggga
     2341 tgaatggttt tccttgatca tcaatgccaa taaggtatcg aagccaagca gcgagagtca
     2401 atggtatgaa tacaagtgtc tgcgggtctt taacttctat ttccatatat tttttaatcg
     2461 tctcaccata acgaatcgcc accttttgag aggtatccgt ggcgattcgc tgaggcatat
     2521 taggaatatt tggattagaa aagcgcacag taattacttc ttccagaaag atctttggat
     2581 caatgatacc tggagactcc acaactggga ggccttcatt gtacccgatt tcagaaacta
     2641 gcagcgataa atctggatct ttcatctcct cagctatgct cctatacccc agtaaacaac
     2701 ccataatagc taaagacgta tgcaaaggat taaggcaagt cgtcaccttc atcgtatctg
     2761 ctttatctac cgttgcacga tcagtcaaga ataccccagc tttttcgaat ttcggacgtc
     2821 cattgggaaa atgatcttcc atgattaaat aatggatgtc ctctgtattg acaaatggag
     2881 caatattatt tccttttgcg gtttggaaaa tcgacatatt tgaaaagccc tcttttgcca
     2941 acattttgct aactgacaag gaaggattcg gggtgatacg atcaatcatc gtccatggaa
     3001 acgcaacaat ttcttcattc gataagtatt ggataaatgt attttgtaca taaccttcct
     3061 tttgccattc ttttgcgatc gttagcaaag aagtacgtaa ttgactaccg ttttgtgaaa
     3121 aattatctgt agagacaagt gcaatcggcc aagctccaga taaaaaacgt ttataaagta
     3181 gcacagcaag aatactcatt gtatgtgatg gcttctttgg accatttttt atatcgactg
     3241 caatcggtcc tatatattta ttatttgtat ctttcaatcg ataacctttt tcagtaatag
     3301 caaaagtaac taattgtaaa ctaggatttt caaagtgtcg aatcatctta tcaaagtgtt
     3361 cagaacaatc tggatgacaa tacagactgt cagctgtcgc agctaatata cgtttgacga
     3421 actctccact gcttggcatg accacttcaa gaatcaaatc atcaaaatct tggtaagccc
     3481 tttcaattat ttcttcatcg aatgtttcac aaacaatgac accagtggac aattcgccta
     3541 gatccgctaa acgttgagcg atttctgcat gaaatcctcg ataaagattt ccgccaccga
     3601 aatggatcca gacaggattt ttttgtccat tttctcttaa tgatgcacgg tcaaaatgag
     3661 gtatttctac ttctttttga gataattcag ggataaagtt cctccagttg tttaaatcaa
     3721 tcatttttcc cctcctaaac taacatatta gtttttgtat tcaagtgaat tttatgcaat
     3781 aaaaatcgct atgtcaacca aaattttact atttatacaa atttactagt taatgtgaca
     3841 taccagattg tttttcagac ctcttctgtt atactacgtt catagaaatg attttggagg
     3901 aataaagatg aaaagcagct tgcaatcaca agcttatcaa gcgattcgta aacaaattat
     3961 ctatgctgac ttagaacctg gaaggaaaat atctgaaaag gcactggagg aacatcttgc
     4021 tatcggccgg actcccattc gcgaagcatt gattcaacta cgtcagcaag aactcgtgta
     4081 tacgatacct caaagtggta cgtatgtttc caaaattaat cttgtttctg cagaaaatgc
     4141 tcgctttgtc cgagaacagt tggaagagca aatcatgatt gaatgttgtt ccaaattgga
     4201 cacacaaaca cgaatagtgc tagaaacgat tttagaagaa caagaaaaag cagtcaaaat
     4261 aaatgacgaa cgcgcttttt tccgtgcaga taatttattt catgaagctt gtttcgaaat
     4321 tgcaggaaga aaagaagttt ggaactggtt agatgaccat aatacccatt tagaacgttt
     4381 ccgctggtta cgagtgacca ttcaggggct tccatgggaa acgatcatgg aacaacatta
     4441 tgatttactc aaggctttag ttgataaaaa accagaagaa gctcgatttc aaacgggatt
     4501 gcacttacac atgatgctga aagaacagga aactgttact caacgttatc ccgagtattt
     4561 ccagcagtag cattttcttg cgacctcttc tatttttata attaaccttc gtttttgagt
     4621 aatctttatt aaataacaaa atatccattg actttgcaac tccaactaaa ttacaataat
     4681 tataaataaa taattattat tggaggtttt actatgtcat tgattgggaa aaaaatcgaa
     4741 gaatttaatg cacatgctta tcatcaagga gaatttttag aagtcagtga aaaagatctt
     4801 atggggaact ggagtattct ttgcttctat ccagctgatt ttacttttgt ttgtcctact
     4861 gaattagaag acttacaaga acaatacagt acattgaaat ctttaggtgt tgaagtcttt
     4921 tcttgctcta ccgataccca ctttactcac aaagcatggc atgatacatc agatgctatc
     4981 ggtaaaatcg aatacgtgat gatcggtgat ccgtctcatc agatttctcg tatttttgat
     5041 gttttggatg aagagcaagg attagctcag cgaggaacat ttattattga tccagacgga
     5101 atcgttcaag ctatggaaat caatgcagat ggaatcggtc gtgacgctag tgcactgatt
     5161 gataagattc gcgctgctca atatgtacgt acccacccag gtgaagtttg tccggcaaaa
     5221 tggaaagaaa gtggcgaaac attgaaacca agttttgatc ttgttggaaa gatttaggtg
     5281 gtggatatgt tagacaaaga aactaaacaa aatctcgaac aatatttagc actgattgaa
     5341 tctcctattg ttttttctgt tagtttggat aatagtgaaa atagtcaaaa actagctgaa
     5401 tttacgaagg aaatagcaga aatgtctcct aaaatctctt gggaaaaaag agattcgatg
     5461 cgcacaccta gtttttctat caatccagtt ggcaaagaaa gtggaatcgt ttttgctggt
     5521 atccccctcg gtcatgaatt ttcctctttt ttactggcta tgttacaaat tagtggacgg
     5581 gcaccgaaaa tcagtgaatc tttgagtctt aaaatcaaaa aaataacaca aacattgcat
     5641 tttgacagtt atgttagctt gacttgtcat aattgccccg acgttgtaca ggcattgaat
     5701 atattagctg tattaaatcc aaatattact catacgatga ttgaaggtgg gatgttccaa
     5761 aaagaaatcg atgataaaaa aatcatggca gtgccaactg tgtttttaaa tggagaagaa
     5821 tttagtagcg gacgcatgac gatcgaacaa attgttgaaa aaatcactgg ccctttgatt
     5881 gaagaagagt tgtttgaaaa agaaccttat gatgtattag tgattggcgg tggaccagca
     5941 gctagtagtg cagctatcta tgcagcacgt aaaggaatcc gtacaggact tgtcgctgat
     6001 tctttcggcg gacaagtcgt tgaaacacta gggattgaaa atatgattgg tacctcttac
     6061 acagaaggtc caaaattgat gcagcaagta gaaagtcatg tgcgtagtta ccctatcgac
     6121 attatgatga atcaacaggc cgtttcctta aataaagagc aacattttat taatattggc
     6181 ttagctaacg gtatatcact taaagcaaaa accgccatca tcgcgacagg tgcgcactgg
     6241 cgttctatca atgtaccagg cgaaaaagag tttaaaaata aaggaattgc ttattgccca
     6301 cactgtgacg gccctttatt taaaggaaaa gagattgctg ttatcggtgg tggtaattct
     6361 ggtgttgaag cagcaatcga tttggctggg ttagcaaaac atgtatatgt tctagaattc
     6421 ttaccagaat taaaagcaga tcaaatctta caggataaac tagtctctct tgcgaatgtc
     6481 actgttatca aacatgcagc gacgaaagaa atcagcggta cgaatcaggt agaatctctc
     6541 tcctatatcg atcgccgtac gatggagatg catacactag ctgtttcagg agtattcatt
     6601 ctagtcggct tactccctaa tacggattgg ctagatagaa cgatcgaaat gaatccaaga
     6661 aatgaaatag tgaccgagaa aaatggcgca acgaatatcc ccggcgtttt cgcagctggt
     6721 gattgtacag atagtccata caaacaaatc attgtttcaa tgggttcagg agctactgca
     6781 gctttaggag cattcgatta tctgatgcgc acttgagcta tgaagattgt aaaaagtcag
     6841 agagcggaaa aaaggatggt gacaagaatt ttgtcacaat cctttttgtt tttttgaata
     6901 atcgcttttt cttctctttt tcttatatac tataagtagt aaataatttg aaaaaaagaa
     6961 aggtgactga ttatggcgca aaatcgtatt tttacgacaa gttttgcaag cgtttatcct
     7021 atgtatgtag caaaagcgga gagaaaagat cgtataaaag aggaagtaga tgaaatcatt
     7081 tgttggctga cgggttattc ccaaacagaa ctagaatatc aactacaaaa acaagtgact
     7141 ttcgaacaat tttttgcgga agcccctgtc cccaatcctt cacgttcctt gattacaggt
     7201 gttgtttgtg gtgtacgagt agaagacatc gaggataact tgatgaaaga aattcgttat
     7261 ttagataaaa tgatcgatga gctagcaaaa ggcaaaaaaa tggaaaagat cctcagaagt
     7321 tgatttttcc agagatattc ttctccttcg ctcattttgt aacatttaat ttggtaaaat
     7381 gactagttca cgttaaaata agtcaaagga ggttttctta tatgtttgag acgattaaac
     7441 aaaatattca aaaatacgcc ttattaagag ctgctgttta tattatttct gggattgcga
     7501 ttgtgctaaa cccttctgct gtttttcatt ttatcggtta tctgatcacc gcctatcttg
     7561 ttttgcttgg gttgttgaat ctttttgagg cttaccgttt taaaaggaaa acaggttctt
     7621 ggagtatcgg tctgttcagt ggtctttttt tcctgatctt agctctgatc gtctatgctt
     7681 ttgcacctgc aatcgtttct atcctgccga ttcttcttgg attagctgtt atcatcaaca
     7741 gcttgttcca gttgttcttc agcatgaata caaaagctaa aagttggagt gtttatagta
     7801 gtattctgtt gttgattggc ggattcgtct tattgttcaa tccattcaaa agtttgatgc
     7861 ttctattcca aatattcggc tttattttga ttttcatggg catccaggag attatcaatt
     7921 tcatcagaat aagaaaaatg actatacgtt aatcattttt agttattaaa atagaccgta
     7981 taaaaaccaa caatccaaaa aaatcttttg gattgttggt ttttttgtga gtatattttc
     8041 ctattttttc aataatgtat gttatgctaa gttcgggcta tattttatac aaaggaatga
     8101 tgagaatgga atttattggt attttatgcc tgatattgat ttcaacaacg attggctcac
     8161 atatctcgcg gagattaggt atcccagcag tgattggaca attacttgtt ggtgttttgc
     8221 taggccaagc tggattgaat tgggtcaaac ctgatattct agtccatgac ttttcggaga
     8281 ttggtgtgat cttattaatg tttttagctg gtctggaaag tgatttatct cttttgaaaa
     8341 aatatttccg acctggtatg ttcgttgctt tgcttgggat catcttcccg gttgtttttg
     8401 gctggttgac aggcaaagca ttccaagttt cgggaaatga agcgattttc tttgggatta
     8461 ttcttgcggc aacttctgtc agtatttcag tagaagtatt aaaagaacta aacgtagtga
     8521 atacaaaaga aggatcaaca attttgggtg cttctgtggt agatgacatt ttagtcgttt
     8581 tagttctaag tttcagccta tcttttctaa cgggggaagc ttctagcaat gcttctttac
     8641 cgcttgtttt gattgaacag ctaatttatt ttgtttttat tttcttatta gtcaaatgga
     8701 ttgcgccttt cctactttca tttgcagaaa aagtgtacgc aaattcttct attatcatcg
     8761 tatcgctagt tatttgttta ggaatgtctt acgtagcgga tcttgttggg cttagctctg
     8821 tcattggtgc tttttttgct gggatagctg tcagtcagac aaaggttaaa gaagaagtat
     8881 accacaatgt agaggcttta ggatatgcag tatttatacc agtatttttt gtcagtgtag
     8941 gattagaagt tgatttttct gactttagtg aacaactact ctttatttct atattgacgc
     9001 ttgtagcgat tttgaccaaa ttgcttggag gttattttgg tgctcagatt gccggttttt
     9061 ctcaacatag ttcattgatg gtcggtgctg gtatgatttc gcgtggagaa atggctttga
     9121 tcattttaca aattggacag caaagtcaat tgatcgaacc acagtactat tcacctttag
     9181 ttatcgtgat tttattaagt acacttattt ctccacttgt tctcaaatat tttacaaaaa
     9241 aagtttatga agtgtgagta aatcaaaaag aagttgtgaa agagacatcg tgaaaaccgt
     9301 ttatttgaaa aaaggactgt gacgataacg ccacagtcct tttactgtac ttaaaaagca
     9361 attttttatt gataattttg gttgaggtat tgcaatagtt ccgatgctcg tttggttgct
     9421 gaatcatcat cttctttatt ttccaacatg ctgaccatca tataaccttg gttatcaaga
     9481 ttgaaagcaa acaagaaact gttctcttgc ccctttacat cctgtttttc tttgatttcc
     9541 gctgtaccag ttttcgctgc caacggaatc cctaaagcag aaagagaatg tgctgtacca
     9601 tttacatctt gcacaacttc tctcagatct ggcacgatca tttgtactgc tgtttcgctg
     9661 atgacattct tcttatcttt tgtctcttta tctgcaatca atttaggata gacaagtgtg
     9721 ccattgttgg taaaaacaga atacattgct gcttgctgga taggagtaat tagaagttcg
     9781 ccctgtccat atccagtatc agctagcaag atatctgagt taaagctatc ttcattagaa
     9841 atttgtgctg gattcatact aattggcaaa tcaaggtctt caccaaaaat gaatttatct
     9901 aagcctgtac gaaatttttt ctcacccatt ttcaacgttt cttgtgccgc atatatatta
     9961 tcggaataga tcaaagcagt ttttaagtct acttgtgata catcactact gacacgcgtt
    10021 gttttaaaag atccccaaga actgtctttt tgccatttaa gcccgttgat cgtcaacact
    10081 tcatttggat cgatagtgcc gttgtcgaga ccgattgctg ctgtaatcat tttaaacgta
    10141 gatccaggag cataacctgt cgcaaatcgg ctgatgaatg gatgttcagg attttcttca
    10201 taagctttgt aatcttcttg tgagatcccg tttgtcattt tgtttggatc atagcttgga
    10261 gagctagcaa gcgcaagaag atcaccggtt tttggcgttg tcgcaacagt tgatgcagct
    10321 tttcctccta gactgtcaaa agctattttt tgtgccttcg catcgattgt caatttgata
    10381 tctttgccat tttgaacttc atgttcgatc agaacttttt tctctacacc gtctgcatct
    10441 gtaatgctta atttcccacc tgttgtccca cgaagatcct tatcaaaagc catctccaaa
    10501 cctgagcgac cgatttttcc attactgctt aattctggat tcttatcaat atcttctgct
    10561 gtgatatctc ccacgtaacc aatcagctga gcagctgctt cacccaaagg ataatatctg
    10621 ccgtctactt cttgaatggt agctccagct ggaagttctg gcgttgctcc atcaatgatt
    10681 ttcaatggga caaagtaatc gggttgtacc cagctttgtg aaatcgcctg attgatagca
    10741 tcttctgtta agtcgaagga ggaagcaatc gctttgatat tggctgtttt ttcgcctcca
    10801 tctccaagtt tacttggaac gactcctaat tgttttagtt tgcctgttgt tgctaatggt
    10861 tcaccatttc gatctataat attccctctt gctgcttctt gtgtggtcag gcttactttg
    10921 tcatttcctt ccatttctgg aaaaaccaag ttgggttgcc agttgatcgt ggtttgacca
    10981 tcatttctgt ctaatgttcc tttataagaa agatctttca attcacctaa tgaggtattc
    11041 atctttgctt tatatgaaaa actgtaagta gaatcgtctt ttttatctac ttttagattc
    11101 gatatctgaa gtcctttgac atcagcagca ccgtatatat tctggtattt atctaagatc
    11161 tctttttcag ataatgcact tttatttgcc gccttttttg atgtcatttc tgcagctttg
    11221 ttataatctc ccttgttcaa agcttggaca aattgctcaa ctgtcttttc tccagcttct
    11281 actgcttggg tttcttgata gtgctggtag taaaaataac cgccacccgc agctgctatt
    11341 aagatcactg cgccggcaat ataagcgcct gttcgatttt tgccgtgctt gtcacttctt
    11401 ttcatttttt cctccttctc ttgcttgtca ttcctttatt tcactattta tacctgttta
    11461 ttctaacata tgtctttgag gaaatccata ccacaatcca gaagcacact ttctggaagg
    11521 ttcgtttgga tactaccaag taatttccca accgctttgg gtagcttgaa tattgaagtt
    11581 acgtctttca gctgctgaga gatagctttg atcacttgct gctgccttct gatcctcccg
    11641 aagtctcctt ctttgtcttc ccgaaatcga gcatactgga gtaaagtatt accatccatc
    11701 acttgctctc ctttgtttat atatacgcca tctaaatcta gagatttttc tgcatcgatc
    11761 ttgactccac caggaaacag ttcattaacg caatcgataa aatcttgaaa tgtgatactt
    11821 gcataatagg gagcttcaaa tttgaaattt tcctttaatg tttgatttaa taaatcaact
    11881 cccccataag agtaagctgc atttatctta ttcattccgt aacctggaat atcgacatat
    11941 gaatctctca tgatagagat cagttttgct tgttgtgttt tctggttgta ttgtgcaacc
    12001 agaagtgaat ccgagcgacc tccaccaatc gatctttttt caccgcatct cctcctcaca
    12061 cagcaatcca cttgtcaggt gaggagcgag tttgtctcat caaataagtc agtcacaaaa
    12121 atataaagaa aaggtcagaa accttttcga acaagaattc tgacctagca tcatctcatc
    12181 gtccgagttt tagcactata caacgtaaag aaaacattct tagctattgg aaaaagcctt
    12241 aatccaacag tttctgtttt acccattggc gactttcgac gtttctgggc aacagcctat
    12301 gtttgtatag gagcctcacc taacaaggtt attactatat tctatttacg agttcatgat
    12361 agacttttcc taatgattag gcaatggaat ttgaaatgaa atagtgaaat ttaacaatct
    12421 ttaatatttt ttagaaaaaa ataacaaata agttgaaaga atcccgttgc aaattctttc
    12481 agttgcacca ggattctttg ataatttttt agttttatgt gtttttttat atgttttttc
    12541 acttttcgga ataaatattt atttgttaaa gtaattaaac aatccgctaa tcgtttgatc
    12601 tggcatttct atttccttac catctaaagt aacagtagta acgacaaaat ggtcttgcaa
    12661 tggcggaact tctaagtact gcccttgcag aaaatcattc actgtttcat attctttttt
    12721 catttctttt gaatctgtcg atggtttata gcttattttt aacataacta gtgctccttt
    12781 ttattcggga cgtgcaatcg tgaaaatttc tcctattgct gcataatcat ttcctgccaa
    12841 acggcttaca gcattcaatt ttcttggatc gatccttcct gtcttatcat catagactgt
    12901 ttcatttaaa tgatatttct gtacttctag caaaaataaa tcagctgtta ctttattttt
    12961 attccgtatc tcgacatgtt catataagga tgtttcaaaa cgaacttttg cttctgtcaa
    13021 tgctggtact gaaatgatta ctgaatcagt taaagtcaag tcagtcaggt acagttcgct
    13081 ttcctcatta ggcagattgg ctgctgtttt atttacttct tctacgttgt cctcatcaag
    13141 gatatggata acagcttctc ttgtctcaag aatattcttt gctgtgtctt ttactgcatc
    13201 ttgttttctc tggatcgcca aggataagat gggtcgattc gaactgacga tattgaagta
    13261 actgaagggt gcgatattca ctatatcttt tgctgattta gtcgttacta ctgctactgg
    13321 tctagggatg actgaaccaa tcagcaactt gtaattttca cgttctgtca attctgaagg
    13381 actccgttca aacatggttt cgccttcttt ctataaaatt cgttaaaaag cagatcttga
    13441 agacaaaatg aaaagtaatg cttttttcat cttcttagaa tctgctttaa actatctatc
    13501 aatttttctt ttttgtcaag aatctgtgcc gtgcgtgttt tttagttatt tgctttactt
    13561 gcggcttcga catatctttt agtgatcaat tgcggacggg tgataataga tccaactacg
    13621 actgaaaaag cacctaattc atatgctttc tctacttgtt caggagagtc agtatgtcct
    13681 tccataatta ctgggatatc gacttgttct atgatttgat tcaataaatc aaaatcaggt
    13741 gtctgcgtct ttcgtgaata ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa
    13801 ccatcttctt gcgcttgaag cgcttcctca aaagtcgaaa tatctgccat agctaaccga
    13861 ccgtgttcgt gaatcatttg gacaagttca gcagttgttt tttctgtcgg acgtttacga
    13921 gaagttgcat ccagtgcaat gatttctgct tgcgtctcca aaagttcttc cacttctttt
    13981 tctgtcgcag taataaacac ttctgaccca tcgtaatctc ttttgataat ccctattacc
    14041 ggtaaatcaa cgattcgctt gatttcattg atatcttctg tcgtatttgc tcgaatacca
    14101 actgctccgc cttcttttgc agccaaagcc attctcccca taataaaaga actgtgtagc
    14161 ggctcgtttt ccaaagcttg acaagaaacg acgactccgc ctttgatttg ttccagtgta
    14221 tccacaatat ctcctccagt aatgttatat aaaaatacag cttcatataa aaatgtaacc
    14281 cttttctatt caggagacaa taatgagcta tttctttcag cagacctttc ttactaaaag
    14341 aaaattactt caaattcttc gtatacgacc agcttctctt ctgtaaattc tatgttcaac
    14401 gcttctttat aagcatgttt aaaaaactca gcatggactt tccgccaata agctaatgtc
    14461 cgatcccctt ctccttcttt ccaagcatgc tctttcgtga cttcactaaa aggcaagatg
    14521 gtcacattta tatttttgat gatacaaact ggttcatttt ttccattcag gaccacgtct
    14581 aattgaccaa cttttggtaa aggctcattc tcttgtttat acagatcgta accacttgat
    14641 gtaccagtct ttatcttttt tacaactagt tcgctcaact cgtctgccat tttttctgta
    14701 tccccaaatg aaaagaaacc taatttttct gattgataac taggatattt ttcttggaac
    14761 tttttgacat attcttctac ttgctgatac ataacatcca cccctttgct ttttataatg
    14821 aaaaaaccat ccagatacta gtataaacaa ttatctggat ggtgagcata atgaattatt
    14881 gaatattcaa gacacgcaga ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt
    14941 cataaagctg ccctacagga acgctatccg tgaattcttt ctctgcacca aaatttaata
    15001 cgttcattga agaatcacca taataacgac tgatattttc gccaaatcct cctgacaaac
    15061 taccatcttc taatgtgatg actaattcgt gtgtttcttt taatttatct aatgtttcta
    15121 cgtcattttc tgtgatcgtt cctggattga tcaacgttgc ttcgattcct ttttttctaa
    15181 aagcttttac tgtttctttt cctaattcaa gaaaaccgcc taaagctaaa attgctactt
    15241 ttcctccatt atttactgtt tgataggctg gaatactata atcatttgcg atagctgtac
    15301 catgttctac accatgtgta ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg
    15361 cccaatctag catagagatc atatcctctt ttgttgctgg agcaagatat ttgatgttcg
    15421 ggatacttga taagtaagga atgtcaaatg ttccttggtg tgttcggtca ctagctgaga
    15481 tcgttccgcc accgactaaa ataatcactg gattctcgtt gatcgctaaa tcatgtgata
    15541 attgatcata tgcccgttgc aagaaagtac ttgattggaa gacgactgga cgtactccct
    15601 gagaagccaa agcagaagcg aaagtgatag agtgctgttc agcgatcccc acgtcaaaat
    15661 aatgatctgg atgtttttct tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat
    15721 tgactgcggc aataggagct gtacttgttt gcattttctt atccaaataa tccatgatga
    15781 tcatattaaa tgtttctcca gaagttactt gctttgcctc cccggtggac agatcaaatg
    15841 gtacatgcca atgatatttc attttattat cgattgccgg ctgataaccg cgaccttttt
    15901 ccgtatggac gtgaagtaca atcggatgat caatatcttt tacttcttca aataaacgga
    15961 tcaccgtttc cagatcgttt ccattatcca cgtaacgata atcaaatccc attgctttaa
    16021 ataggttatt gctgctttta ccgttggttt gacgaagctc agctaagttt ttatataaac
    16081 tgccatgatt ctcatcaata gacatctcat tatcgtttac taatacgatt agattggatt
    16141 gtaaaacacc accattgttc aatccttcaa aagctaagcc accacttagg gagccgtcac
    16201 cgataaatgc taccacattt cctgattctt tcttcagatc tctcgcttta gctaaacctg
    16261 ttgctaagct gatagatgta gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat
    16321 gttggttgga atatccgctc acttcacggt aatgtccttt ttcaaaacca ttttttcgac
    16381 cagttaaaag cttatgacca taagactgat gggaaacatc ccaaacgatt ttatcctttg
    16441 gtgaatcaaa tacataatga aacgcaatcg ttaattctac tacacctaga ttcggaccaa
    16501 cgtgcccacc tacatcgctg tccttttgca agattttttg gcgcatctct tctgccaact
    16561 gtttcatttc ctcaacagat aattttttga catctgcggg gctattgatt gcatccaata
    16621 catgtaatac tgtactcata atacattctc tcctccgtac tatcttcttt taatgaaaat
    16681 agatttgtta cactttatct attctactac ttagagttga ctctaagtca atagcatttt
    16741 aaaaaaattt cagagaaagc ttgttatata agtagatctt cgcacgaatc aggactcaaa
    16801 taaaaacctt ctacagacaa ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat
    16861 caagaaatat gtttttttaa taggtgaagt tcctgagcaa tatgatacaa atggatgttt
    16921 gcacctttta atgagattgg atagatcttt gtcttttcag tttctaatcc attttgatag
    16981 ctagcagcat cgcacaaaac cactcttttt ttcgcacata gacattcatc aggcaattct
    17041 tctaatgaac gaaataaaat aggatttatt tcagcgatgc cttcttccgc atacaaatga
    17101 aattgggttc gataaaaaaa ttgtttgact gaactttctg aaacaacaaa aatttctatt
    17161 tcctgtctcc ccgcttgttc actgttgatc aaagaaagac ttttgacgaa ttttcgaatg
    17221 aaattttcat tccatcttag ttggatttga tttttctttt tccactcttc caatatttct
    17281 acaatttttc cgtacagaag ttgttctttt tctgatagaa gatagatcct ttccggtaaa
    17341 aatacttgtc catctgccca caacgtacgg acaaattgta aaaaggattg cttgaaaata
    17401 aaattttctg catcgtcgat ttcagcgata ctaataatct gtaaaatcaa ttcattcagt
    17461 gataggtcat tgagaacaaa atttttataa acaacttcga aatcttttct taacagctcc
    17521 atgttctcat tggtataatt tctactgttg aacaaagaaa aaatataata gacatcttct
    17581 tcttgtaagt ctagaattcc tttttgctct tccaatcctt gacagactaa ttgaaataac
    17641 ggcttcgttt ctgcgctttt cttatcttct tctttcagtc caaggggaaa tgcctttctt
    17701 ccgattgcga tctctattcc tcgtatgata aaggactttt catcttccga atatcttctt
    17761 ttaaaaaaat gagtctctac ataatcaatc aactcgttac aagcttcttc tacatacgga
    17821 tttttatttt catagccttc ccaattgaca taacgtacta aagctagcaa gatgttccgc
    17881 gagtcaaatt ctgggataat gatttcttta tttttcaaat aatcattttc tttaaaaaaa
    17941 tccaatacga tttttttgat tgtatatact ttgctcaaag aaataaattc aagatcagcc
    18001 agttctgtac tcgtgaatat accattaaaa taataactta aaaactttaa aaaataagat
    18061 tgaccgtaca gaaactgtgt gagctcagaa agagcaatac catgtttgaa tacgagttta
    18121 tacttatgct tctctcttac ttgttctttt atacaatatt ctagacactc ggctagactt
    18181 tctagatcgt gaaggatcgt tggtgtagtc acccttagag aaatagctgt ttgttcgatg
    18241 tctatttctt ggaattcaaa taattgttca cacagataca cttttctaaa tatatttcgc
    18301 tcgatgtaat tttccagcat tcctgataca cgcctccagt ttttcttatg attttattta
    18361 atcatatctg taaatcgtat ttttgtttcc tttttattta gaaaagccct ttcaaaaacc
    18421 aataaaaaaa gaaactggga caaaagtcaa acaagctcac aatttcagcc tcattgtagg
    18481 tagctttgag ccgtttggac ttttacccca gcctctttca agaatggtta ttcaaagatg
    18541 tcagcgtcta gttctttgac tacatctttc aacaattgga taccagctgc gctgttacca
    18601 aatggatcta gtgccgggct gaagacacct atgccgtaac gatttggcac agctgccatc
    18661 aacccgccgc ctacaccgct ttttgcaggt acaccaacat ggacggaaaa ctctcctgat
    18721 tcatcataca aaccagcagt tgtcataatt gatttgacga ttgtagcact ttcttcagta
    18781 atgatttgtt tgccattcca aggagcaatt cctttatttg ccagcacagc agctaatttt
    18841 gctatgcctg tagcggtgac attgagggag cattgtctga aataagcatc gagtatctcc
    18901 ggtacgtctc cttcgatcat ttgattacct ttcatatagt aagcaagaga gcgattgata
    18961 tcacctgttc ttgattcaga atgatagatt tcttcattca atgtgatatt aggatcatca
    19021 cagatctctt tcataaatga caagattcgc tcaaattttt catctgcatc tttaccggca
    19081 atcaacgagg ttgttgcaat cgctcctgca ttaacaaaag gattcattgg atgtttgcga
    19141 tgattgattt ccatattcat gatagaattg aaggcaaaac cggttggttc cgtgttgatt
    19201 gtttgaaaga ctttttctat tccgttgtct ttgattgcta ataacaatac gggaactttt
    19261 gacatgcttt cgatggcaaa gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta
    19321 tccaaatcat aaatcgccat acctaattgt ttgggatcca cttcagctaa tgctggaatg
    19381 taatccgcca cttgtcctgt ttgataatag tggaaatttt ttccaatcaa ttgttctaat
    19441 tcttttactt ccatttgaat aaccagcttt cattttttat ttttctgcat gctggaattt
    19501 atgacggcat gcgtagatcg taaatggaat aactaagacg ataagataag aaacaatcag
    19561 aatcgtttca taaatcccat gctgtccagc tgcgatacta gcaggtggaa caaatgagat
    19621 acaaaaagca aaaagtgaag tcaaaagacc taaacaagca acgattactt ttacaacttt
    19681 tccgcctgga acctgatagg ctctttcaag actgtcatct ttaaaaacca gcttcaaata
    19741 gcccgcgaaa aacataaagt acaacatcag gtagattaca acagtcaatg acatcgctgc
    19801 aagaaatgaa agattattcc ccccgccgct taatgtaaag acggctgccc agattgttgc
    19861 aacgatccca tttgcgatca tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa
    19921 atattcagga agtaatcctt cttctgctgc tacgtgaagt gcttttgaag gagaggttac
    19981 ccaagaagca atttctccga tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat
    20041 ccatgcaaga tttggatcaa tgtgtcttaa taggaattct aacgattgaa tcacccctgt
    20101 attaagagac aaaccatgtt gaggtactgt agtagcgacg gtcaatccgc caaaagtatc
    20161 taaaatgatt gccagaataa ccaatacaaa cattgctaat ggataatcct ttttagggtt
    20221 ttccatttca tttgcatgac tggcagatgt ttctacgccc atatatgcta aaataaagga
    20281 aacaaaaaca accagcgtag gcaatttcga aaaatcagga atcaatgttt tcatagacag
    20341 tatcgtttgt agtggagcgc ctgaagcgac ataatggatc cctaagccaa acaagactaa
    20401 tgctggcaac aagataccag ctacaaaacc gatccgagca atcaatgaag tgtatttggt
    20461 tccttttaac tgtgagagta aaatagccca gaaaataatc aataggattg ccaatttcag
    20521 atatttattg ttttgtatag caccaatacc cgttgcatag gataatgcac cagttaagaa
    20581 atacagcatg gtcaagtagc caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa
    20641 accgaatcgt tttcccaatg tgcgactgac ccaagtatag acaccgcctt tttcccatcc
    20701 tttcaccgtt gccatctctg ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc
    20761 taataacaag aaaaatacta agctgaagcc tgaggtcgcg aatgtgggat attcatataa
    20821 tgaaacgacc attgagatgg tcatagccaa aaagccaaag aacgttaatt tttttgcagg
    20881 aattttcgtt ttgctttgtt gcatgaatct tcctcttttc tatttgaatt tgtttcaatt
    20941 gagtttgttg ccaaagaaga aaaagtccaa tccttcttct tgttcttctt ttgtagtagt
    21001 gatcgttgta aagtgcatcg ccgattttaa aatcactgtt gtcagcacat aggcatacac
    21061 ggcagtaatg atgacaccaa tcacttgaat ccctaagaag taccagctgc ttggattcgt
    21121 tagtaaatgg ctgttagatg caaagatccc tatcagaatc gtacctgtga atccgcccat
    21181 gccatgaaca ccccacacat caagtgcatc atcccattgt ttctttttac gaaaatcaac
    21241 agcagcatga cagacgatac ccgcaatgat cccaatcagc atggcacttt ttgcttctac
    21301 ataaccggca caaggcgtaa tggctgccaa tccagcaacg gatcctgtta gtacgtcgac
    21361 aaaatcaaag ccatttcctt ttacttttgc aatgatcagc caagtaatca tcgcaaatgc
    21421 aagagcgatg aatgttgaac caaatgcatt gactgcttgt tcatctgctc gaagtgcacc
    21481 accggaatta aaaccgaacc agccaaacca aagcagtcct gtaccgatag cagctgccat
    21541 aagactgcta tgctgacttc tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat
    21601 acatgctaaa gagccgaatc cagctgtcgt gtggatgact gctccaccag cgaaatctct
    21661 aaatcccatc tgatccaaaa atccgccacc ccatatccag tggcaaacag ggaaataaat
    21721 cagcagattc caaaaaataa caagtataat atagcctttt aaattcaatc tccctgcaaa
    21781 agctcctgtc atcaaaggaa cggtaatcac gataaacatc agttggtaaa gaaagaacat
    21841 taaaaatgga atcgttgcac catgaaacat gttggggaag cttgtaacat gtctcatcaa
    21901 aaagaaatct gccgggtttc caataacacc gccgatatcc cgtccaaagg ccaatccgaa
    21961 tccaccaaat acccacataa tcgttgttat cccaacggag agaaaagatt gagacatgat
    22021 cgttagtata ttattcttcc gtgctaatcc gccgtaaaaa aaagctagac caggagtcat
    22081 caggcaaacc attgctgtac ataaaatcat gaatgctgta tctcctgtat tgataggtaa
    22141 tgtattcatt tttttcgcct cctttctacg attacgagta taaaagaagg tgtagttgtt
    22201 tttttcttct gtttttttga attaatactt gcatttataa aagacagaca attaataaaa
    22261 gct
//
LOCUS       DAZUMV010000036        20991 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000036 DAZUMV010000000
VERSION     DAZUMV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.036"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..468
                     /gene="rpsJ"
                     /locus_tag="ACG8FK_001225"
     CDS             160..468
                     /gene="rpsJ"
                     /locus_tag="ACG8FK_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS5096841.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            499..1128
                     /gene="rplC"
                     /locus_tag="ACG8FK_001226"
     CDS             499..1128
                     /gene="rplC"
                     /locus_tag="ACG8FK_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS5096842.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1156..1779
                     /gene="rplD"
                     /locus_tag="ACG8FK_001227"
     CDS             1156..1779
                     /gene="rplD"
                     /locus_tag="ACG8FK_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS5096843.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1779..2069
                     /gene="rplW"
                     /locus_tag="ACG8FK_001228"
     CDS             1779..2069
                     /gene="rplW"
                     /locus_tag="ACG8FK_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS5096844.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2108..2941
                     /gene="rplB"
                     /locus_tag="ACG8FK_001229"
     CDS             2108..2941
                     /gene="rplB"
                     /locus_tag="ACG8FK_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS5096845.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            2984..3262
                     /gene="rpsS"
                     /locus_tag="ACG8FK_001230"
     CDS             2984..3262
                     /gene="rpsS"
                     /locus_tag="ACG8FK_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS5096846.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3285..3632
                     /gene="rplV"
                     /locus_tag="ACG8FK_001231"
     CDS             3285..3632
                     /gene="rplV"
                     /locus_tag="ACG8FK_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS5096847.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3646..4302
                     /gene="rpsC"
                     /locus_tag="ACG8FK_001232"
     CDS             3646..4302
                     /gene="rpsC"
                     /locus_tag="ACG8FK_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS5096848.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4305..4739
                     /gene="rplP"
                     /locus_tag="ACG8FK_001233"
     CDS             4305..4739
                     /gene="rplP"
                     /locus_tag="ACG8FK_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS5096849.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4729..4917
                     /gene="rpmC"
                     /locus_tag="ACG8FK_001234"
     CDS             4729..4917
                     /gene="rpmC"
                     /locus_tag="ACG8FK_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS5096850.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            4942..5208
                     /gene="rpsQ"
                     /locus_tag="ACG8FK_001235"
     CDS             4942..5208
                     /gene="rpsQ"
                     /locus_tag="ACG8FK_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS5096851.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5266..5634
                     /gene="rplN"
                     /locus_tag="ACG8FK_001236"
     CDS             5266..5634
                     /gene="rplN"
                     /locus_tag="ACG8FK_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS5096852.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5671..5979
                     /gene="rplX"
                     /locus_tag="ACG8FK_001237"
     CDS             5671..5979
                     /gene="rplX"
                     /locus_tag="ACG8FK_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS5096853.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6007..6546
                     /gene="rplE"
                     /locus_tag="ACG8FK_001238"
     CDS             6007..6546
                     /gene="rplE"
                     /locus_tag="ACG8FK_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS5096854.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6565..6750
                     /locus_tag="ACG8FK_001239"
     CDS             6565..6750
                     /locus_tag="ACG8FK_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS5096855.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6786..7184
                     /gene="rpsH"
                     /locus_tag="ACG8FK_001240"
     CDS             6786..7184
                     /gene="rpsH"
                     /locus_tag="ACG8FK_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS5096856.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7216..7752
                     /gene="rplF"
                     /locus_tag="ACG8FK_001241"
     CDS             7216..7752
                     /gene="rplF"
                     /locus_tag="ACG8FK_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS5096857.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            7913..8269
                     /gene="rplR"
                     /locus_tag="ACG8FK_001242"
     CDS             7913..8269
                     /gene="rplR"
                     /locus_tag="ACG8FK_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS5096858.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8290..8790
                     /gene="rpsE"
                     /locus_tag="ACG8FK_001243"
     CDS             8290..8790
                     /gene="rpsE"
                     /locus_tag="ACG8FK_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS5096859.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8805..8984
                     /gene="rpmD"
                     /locus_tag="ACG8FK_001244"
     CDS             8805..8984
                     /gene="rpmD"
                     /locus_tag="ACG8FK_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS5096860.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9028..9468
                     /gene="rplO"
                     /locus_tag="ACG8FK_001245"
     CDS             9028..9468
                     /gene="rplO"
                     /locus_tag="ACG8FK_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS5096861.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9468..10763
                     /gene="secY"
                     /locus_tag="ACG8FK_001246"
     CDS             9468..10763
                     /gene="secY"
                     /locus_tag="ACG8FK_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS5096862.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10825..11472
                     /locus_tag="ACG8FK_001247"
     CDS             10825..11472
                     /locus_tag="ACG8FK_001247"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS5096863.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11663..11881
                     /gene="infA"
                     /locus_tag="ACG8FK_001248"
     CDS             11663..11881
                     /gene="infA"
                     /locus_tag="ACG8FK_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS5096864.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            11914..12030
                     /gene="rpmJ"
                     /locus_tag="ACG8FK_001249"
     CDS             11914..12030
                     /gene="rpmJ"
                     /locus_tag="ACG8FK_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS5096865.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12048..12413
                     /gene="rpsM"
                     /locus_tag="ACG8FK_001250"
     CDS             12048..12413
                     /gene="rpsM"
                     /locus_tag="ACG8FK_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS5096866.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12441..12830
                     /gene="rpsK"
                     /locus_tag="ACG8FK_001251"
     CDS             12441..12830
                     /gene="rpsK"
                     /locus_tag="ACG8FK_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS5096867.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            12910..13848
                     /locus_tag="ACG8FK_001252"
     CDS             12910..13848
                     /locus_tag="ACG8FK_001252"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS5096868.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            13876..14256
                     /gene="rplQ"
                     /locus_tag="ACG8FK_001253"
     CDS             13876..14256
                     /gene="rplQ"
                     /locus_tag="ACG8FK_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS5096869.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14528..15367
                     /locus_tag="ACG8FK_001254"
     CDS             14528..15367
                     /locus_tag="ACG8FK_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5096870.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15343..16212
                     /locus_tag="ACG8FK_001255"
     CDS             15343..16212
                     /locus_tag="ACG8FK_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5096871.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16202..16999
                     /locus_tag="ACG8FK_001256"
     CDS             16202..16999
                     /locus_tag="ACG8FK_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS5096872.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17202..17948
                     /gene="truA"
                     /locus_tag="ACG8FK_001257"
     CDS             17202..17948
                     /gene="truA"
                     /locus_tag="ACG8FK_001257"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5096873.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18281..20950)
                     /locus_tag="ACG8FK_001258"
     CDS             complement(18281..20950)
                     /locus_tag="ACG8FK_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS5096874.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         6963 a         3847 c         4537 g         5644 t
ORIGIN      
        1 gcgtttactt tagtgaattg cacagacgta atcagaatga tttcgtcggc taagaaacga
       61 gaggttgcga cacgcccgga cgctttgcca cgaacgagcg tgacggaaat tttcgtggag
      121 ctatgtctac ttttcaaaat aggcgaagga gggaacaaga tggcaaaaca aaaaattcgt
      181 atccgtttaa aagcgtatga acaccgtatt ttagatcaat cagcggataa aatcgtggaa
      241 actgcaaaaa gaactggagc tagcgtgtct ggtccaattc cattaccaac tgaacgcagt
      301 ctttacacaa tcatccgtgc gactcataaa tacaaagatt cacgcgaaca attcgaaatg
      361 cgtacacaca aacgtcttat cgacattgtg aacccaacac caaaaactgt tgatgctttg
      421 atgaagctag acttaccatc tggtgtaaac atcgaaatca aattataaaa ctaaaacaaa
      481 aataatggag gtgtactcat gaccaaagga atcttaggga aaaaagtggg aatgacacaa
      541 atcttcactg aatctggtga attaattcca gtaactgttg tagaagctac tccaaacgta
      601 gtactacaag tgaaaacaat ggaaacggat ggctacgaag ctattcaagt tggttaccaa
      661 gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc atgttgcaaa agcaaacacg
      721 gctcctaagc gcttcattcg tgaattcaaa aatgttgagc taggagaata tgaagtagga
      781 aaagaaatta cagtagatgt tttccaagca ggagacatta ttgatgtaac aggtactacg
      841 aaaggtaaag gattccaagg ggttatcaaa cgccacggac aaagccgcgg accaatggct
      901 cacggttctc gttaccatcg tcgtcctggg tcaatgggtc cagttgcacc taaccgtgta
      961 tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc gcgtaactat ccaaaacttg
     1021 gaagttgtac gtgtcgatgc agaaagaaat gttatcttga tcaaaggtaa tgttcctgga
     1081 gctaaaaaat ctttaattac tatcaaatca gctgtgaaag ctaaataatt gtaggaagaa
     1141 aggaggaact aaggaatgcc gaatgtagca ttattcaaac aagatggaag ccaaaacggt
     1201 gaaatcacac ttaacgaaga aatcttcgga attgaaccta atgaaagtgt tgtttacgat
     1261 gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac acgcggttaa aaaccgtagc
     1321 gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag gaacaggtcg tgcacgtcaa
     1381 ggatctatcc gctcaccaca atggcgtgga ggtggcgtag tctttggacc aacaccacgt
     1441 tcttacagct acaaacttcc taaaaaagtt cgtcgtttag caatgaaatc tgtattgtca
     1501 gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct tgaacttcga cgcaccaaaa
     1561 acaaaagaat tcaaacaagt tcttgctaac ttatctatcg actctaaagt attggttgtt
     1621 ctagaaaaag gtaacgactt tgctgcttta tcagcacgta acttaccaaa cgtttctgta
     1681 gtaacttctg ataatgtgag tgtgctagac gttgtatcaa atacaaaagt gttagcaaca
     1741 caaactgctc ttactcaaat tgaggaggtg cttgcataat gaacttacta gacgtaatca
     1801 aacgcccagt gatcactgaa aaatcaatgc ttgccatgga tgacaagaaa tacacttttg
     1861 aagtggacac tcgcgcaaac aaaactttag taaaacaagc ggtagaagct gcttttgacg
     1921 ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa attcaaacgt atgggcaaat
     1981 atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac attgactgaa gattcaaaag
     2041 aaatccaatt attcgaagct gctgaataag cactaataga taactcatta aattaggagg
     2101 gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat ggtcgtcgta acatgacagg
     2161 ttctgatttt gctgaaatca caacatcaac acctgaaaaa tcattgttac aaccattgaa
     2221 aaaccatgct ggacgtaaca acaacggccg catcactgta cgtcatcaag gtggcggaca
     2281 caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa gataacgttg ttgcaactgt
     2341 tcaaacgatc gaatatgatc caaaccgttc agctaacatc gcgttagtac actatgaaga
     2401 tggagtgaaa gcttacatct tagcacctaa aggactagaa gtgggtatga aacttgtttc
     2461 aggtccagaa gcagatatca aagtcggaaa tgctcttcca ttagaaaaca ttccagttgg
     2521 tactgttatc cacaacattg aaatgaaacc tggtaaaggt ggacaattga tccgttctgc
     2581 tggtacaagt gctcaagtac ttggtaaaga aggcaaatac gtattgatcc gcttgaactc
     2641 tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca atcggttctg taggtaacga
     2701 acaacacgaa ttaatcaaca tcggtaaagc tggacgctct cgttggatgc gtaaacgccc
     2761 aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca cacggtggtg gtgaaggtaa
     2821 agctcctatc ggacgtaaag caccagtttc tccttggggt caaccagctc ttggttacaa
     2881 aactcgtaac aaaaaagcta aatcagacaa acttattgtt cgtcgtcgca aaaataaata
     2941 attactttga ccatgtgtcg cacattttga gaggaggttc accatgggtc gtagtttaaa
     3001 gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt gaagctcaag caggggttga
     3061 aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca gttttcccaa gttttgttgg
     3121 atataccatc gcagtttatg atggacgtaa acatgtacca gtttacatcc aagaagacat
     3181 ggtaggacac aaattaggtg aatttgcacc tacaagaact tatcgtggac atgctgctga
     3241 cgataagaaa acgaaacgct aatttgaggg gaggataaat ccaaatggca gaacaaatca
     3301 catcagctaa agcaactgca aaaacagttc gcacttcacc tcgaaaagcc cgtttagtaa
     3361 ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc aatcttgaaa ttcacaccaa
     3421 acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc agttgcgaat gcagaaaata
     3481 actttgactt agatgttgaa aacttggtag taagcgaagc ttttgttaac gaaggaccta
     3541 caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc tccaatcaac aaacgtacaa
     3601 gtcatatcac ggtagtcgta tcagaaaaat aaggagggat aatcagtggg tcaaaaagta
     3661 catccaattg gaatgcgtgt aggcatcatc cgcgactggg atgctaaatg gtatgctgaa
     3721 aaagagtatg ctgagttctt acacgaagat ttaagaatcc gtaaatttat ttcaactaaa
     3781 cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg caacaggtcg cgtaaacatt
     3841 tcaattcaca cagctaaacc aggtatggtt attggtaaag gcggttctga agtcgaaagc
     3901 ctaagaaaag aattaaacaa attaactggt aaaagaatcc atatcaatat cgtggaaatc
     3961 aaaaaaccag atctagatgc aaaattagta ggtgaaggaa tcgcacgtca attagaaaac
     4021 cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac gctcaatgcg tgctggcgct
     4081 caagggatca aaactcaagt atctggtcgt ctaaacggag cagatatcgc tcgttcagaa
     4141 ggatactcag aaggaactgt tcctcttcat actttacgtg cggacattga ttacgcatgg
     4201 gaagaagcag atacaacata cggaaaacta ggagttaaag tgtggatcta tcgtggagaa
     4261 attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat aatcatgtta gtacctaaac
     4321 gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg ggaagctaaa ggcggaaaag
     4381 aagtagcatt cggtgaatac ggtttgcaag ctgttgattc acattggatc acaaaccgcc
     4441 aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa acgtggtggg aaagtatgga
     4501 ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat tggggtacgt atgggttctg
     4561 gtaaaggggc acctgaagga tgggttgcac cagtaaaacg tggtaaaatc atgtttgaaa
     4621 tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg tctagcttct cacaaattac
     4681 caatgaaaac taagatcgta aaacgtgagg aaatgggtgg tgaatcgaat gaaggttaaa
     4741 gaaatcagag aattaaccac tgccgaaatg cttgatcaag aaaaacaatt aaaagaagaa
     4801 ttgtttaatc ttagattcca attagcaaca ggtcaattag aaaacactgc acgtattaaa
     4861 gaagtacgta aatcgattgc acgcatcaaa actgtgttgc gtgaacaagc taagtaatta
     4921 tggaaggagg ccattaagcg tatgactgaa ggaagaaatc aacgcaaagt ttatcaaggt
     4981 cgcgtggttt cagacaaaat ggataaaaca attaccgttg ctgtcgaaac aaagaaaaac
     5041 caccctatct atggcaaacg catgaactat tctaagaaat acaaagtgca tgatgaaaac
     5101 aacacagcaa aagttggcga catcgtgaga attatggaaa ctcgtccatt atcagctaca
     5161 aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta ttatctaata acacgagatt
     5221 ttcctatata gattgaatgc aaaatctgaa aggaggatac acaacgtgat ccaagcagaa
     5281 agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa ttttgacgat caaagtcctt
     5341 ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga tcgttgctac ggttaaacaa
     5401 gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta aagccgttat cgttcgaaca
     5461 aaatcaggag ctcgtcgtac agacggttca tatattaaat ttgatgaaaa tgctgcagtg
     5521 attattcgtg atgataaaag ccctcgtgga acacgtatct ttgggccagt tgcacgtgaa
     5581 ctacgtgaaa acaacttcat gaagatcgtt tcactagcac cagaagtttt ataatcttga
     5641 gacacttatc aaaggaggtg cgaaacagtt atgtttgtta aaaaaggcga taaagttaaa
     5701 gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac tagcagcgtt cccgaaaaaa
     5761 gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac accaaaaacc ttctcaagct
     5821 gctcctcaag gtggaattgt tgagatggaa gcgccaattc atgtttctaa cgtgatggtg
     5881 gttgactcta ctggtgtagc tggccgagtt ggctacaaag aagtagacgg caaaaaagtc
     5941 cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa agtttaagga aggagggtct
     6001 tactgaatga accgcctaaa agaaaaatat cttaaagaag taactccatc attgatggaa
     6061 aaatttaact atagctctgt tatgcaaaca cctaaagttg aaaaaatcgt catcaacatg
     6121 ggtgttggtg atgcggtatc aaacgctaaa aacttagata aagcagttga agaattaact
     6181 ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa aatcaatcgc tggattccgt
     6241 ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc gcggagaaag aatgtacgaa
     6301 ttcttagaca aattggtttc tgtttcacta ccacgtgtac gtgacttcca tggtgtaagt
     6361 aaaaaagcct ttgacggtcg tggaaactat actttaggta ttaaagaaca attgatcttc
     6421 ccagaagtag attatgattt agtagataaa gtacgtggta tggacatcgt tattgtaaca
     6481 acagcaaact ctgatgaaga atctcgtgag ttgttgacac aattaggcat gccattccaa
     6541 aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa tgattgctaa aaacaaacgt
     6601 cctgcaaaat tttcaacaca agcttatact cgttgcgaac gttgcggacg tccacattca
     6661 gtttatcgta agttccatct ttgccgtatt tgcttccgcg aacttgccta taaaggtcaa
     6721 attcccggcg tgaagaaagc tagctggtaa aaagtaaatt cgcataagaa ggaggtaaaa
     6781 gatcaatggt catgacagat ccaattgcag attttctaac tcgtatccgt aacgcaaaca
     6841 tggttaaaca tgagtcttta gaagtacctg catcaaaaat taaacgtgac atcgctgaaa
     6901 tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat cgaagatgac aaacaaggcg
     6961 tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg tgttatcaca aacttaaaac
     7021 gtatctctaa accaggtttg cgtgcttatg tcaaagctga cgaagtacca aaagtattga
     7081 acggtttagg tatcgcaatc atttcgactt ctgaaggtgt tgtcactgat aaagaagctc
     7141 gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg gtaatcaagt agaatctaca
     7201 aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg ttgttatccc tgaaggagta
     7261 acagttactc aagacggaaa caacgttaca gttaaaggac caaaaggtga attgactcgt
     7321 actttttcag cagatatcaa aatgaatatc gaaggaaacg aagtaacttt cactcgtcca
     7381 aacgacagta aagaaatgaa aacaattcat ggaacaactc gtgcgaactt caacaacatg
     7441 gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac taatcggggt tgggtaccgt
     7501 gcacaattac aaggatcaaa acttgtattg aacgttggtt actcacatcc agtagaaatc
     7561 acaccaccag ctggagtaac tgttgaagta ccagctaaca cacaagtaat tgttaaaggt
     7621 gcgaacaaag aagaagttgg cgaattagct gcaaacattc gtggaactcg tcctccagaa
     7681 ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg tacgccgtaa agaaggtaaa
     7741 actggtaaat aatcgtttag aaaaattgca tagaagccag cagcaggaaa aaggcatttg
     7801 gctgaagttt accttcaaaa aaatacattt ttacttgtgc cgcagcttct atttttcgaa
     7861 acgtatttac tgcttgaaat agtagtacaa taaaaataaa gaggtgacaa ttgtgattac
     7921 aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt cgtgtacgta acaatatctc
     7981 tggtactgct gagcgcccac gcttgaacgt ttttcgttct aacaaaaaca tctacgctca
     8041 aattattgat gacgtagcgg gtgtaacgct agcaagtgcc tctaccttgg ataaagaaat
     8101 ttcaggtgga acaaaaacag aagcagcaca agctgtcggt aaactagttg ctgaacgtgc
     8161 agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt ggataccttt accatggccg
     8221 tgtgcaagct ttagctgaag ctgctcgcga aaatggacta gaattttaga agaaggagga
     8281 ataccattca tggtttatat cgatccaaaa catttggaat tagaagaccg cgttgttgca
     8341 attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc tacgttttgc tgctttagtt
     8401 gttgtcggtg acaaaaacgg acacgtaggg tttggtacag gtaaagctca agaagttcct
     8461 gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact tagttgaagt acctatggtt
     8521 ggttcaacaa tcccacacga agtaatcggt gcttttagtg gtggtagaat cctaatgaaa
     8581 cctgctgtag aaggttctgg ggttgctgct ggtggaccag ttcgtgccgt attagaatta
     8641 gctggggtag cagatatcac ttctaaatct ttaggctcaa atacaccaat caacgttgtt
     8701 cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag aagaagttgc agaacttcgc
     8761 ggcaaatcag ttgaagaatt aatcggataa ggaggacaac aataatggct gaattaaaag
     8821 ttactttaaa acgcagtatt atcggacgtc ctcaaaacca acgcgataca gttaaagcgt
     8881 taggtcttac aaagattaac agttcagtgg ttaagccttc taatgaagca attaaaggca
     8941 tgatcaatac tgtttctcac ctagtggacg ttgaagaaat ttaatttaga aaaatcataa
     9001 ctagaacatt aaggaggtgc caaacctatg aaacttcatg aattaaaacc tgctgaaggt
     9061 tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat ctggtaacgg aaaaactgcc
     9121 ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg gtgtgcgttt aggatttgaa
     9181 gggggacaaa caccattgtt ccgtcgttta ccaaaacgag gattcacaaa cgtgaaccgt
     9241 aaagactatg cagtcattaa tcttgatgta ttgaaccgct ttgaagacgg agctgaagta
     9301 actcctgttg ctttagttga agctggaatc gtgaaaaacg aaaaagctgg aatcaaagta
     9361 ttagctaatg gggaactaaa caagaaatta actgtgaaag cagcaaaatt ctctaaagca
     9421 gctcaagaag caattgaagc ggctggtgga tcaatcgagg tgatctaatg tttagactat
     9481 taaaagatgc ttttaaagta aaagacatta gatcgaaaat cctatttact gtatttgtgt
     9541 tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg cgtaaatgct gcttcattgc
     9601 aaaacctttc ttctttgcca tttttcaaca tgttgaactt ggtaagtggt agtgcgatgc
     9661 aaaacttttc cattttctca atgggggttt caccatacat tactgcttct atcgtcattc
     9721 agcttcttca aatggacatc gtgcctcgat ttgttgaatg gtcgaaacaa ggggaagttg
     9781 gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt agtattggca tttatccaat
     9841 ctgtcggtat cactgctgga ttcaatcaat acgctcaatt aggtttcgtg aataatccta
     9901 acgtagcaac ttacgtaacg atcggtttga ttctaacagc cggtacgatg tttgttactt
     9961 ggctaggaga acagatcaca gaaaaaggta tcggaaatgg cgtatcaatg atcatttttg
    10021 caggtatcat ttctagattg cctgaaagta tcagagaaat ttatgaagat tactttataa
    10081 atattgatcc ttcagacata tggaaatctg ctatttttgt ggccatttta ctagtagcgg
    10141 tcttgttgat cgtgacattc gtaacatttt tccaacaagc tgaacgcaag atcccgatcc
    10201 aatatacgaa acgcgtagct ggcgcgccta caagcagcta tctgccgttg aaagtaaatg
    10261 cagctggagt tattccagtt atctttgcta gttcgtttat tgctacgcca aatgctgttt
    10321 tacaagcatt gagcggtaga ttcggttctg aaaactggta caaagtgatg atggagatct
    10381 ttaactataa tacagcacct ggtgctacga tttatacgat tctgatcgtt gccttcactt
    10441 tcttctacgc ctttgttcaa gtaaaccctg agaaattagc ggaaaacttg caaaagcaag
    10501 gaagttatat accaagcgta cgccctggta aggggactga agagtatgtc tcaagattac
    10561 tgatgcgttt aagtacagtt ggtgcagtct tccttggttt agtggcattg cttccaatca
    10621 ttgctcaaat ggtttgggat cttccacaat caatcggatt aggcggtaca agcttactga
    10681 tcgttatcgg tgtagcttta gaaactgcga aacagttaga aggcttaatg ctgaaacgta
    10741 aatatgttgg attcattgag taagaaagcg tgttgaggtt tcccctcaac acacgttctc
    10801 acacatttat taggagggaa aacaatgaac ctcattttaa tggggctgcc aggcgcaggt
    10861 aaaggaacac aagctgaaaa aatcattgat gcttatggta ttccgcacat ctcaacagga
    10921 gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg gcctggaagc aaagtcttat
    10981 atggataaag gtgcattagt tcctgatgaa gtaacaaatg gaatcgtgaa agagcgacta
    11041 gctgaaccag atacagagaa aggcttctta ttagatggct ttccacgtac gctagatcaa
    11101 gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa aaattgatgc tgtcatcgat
    11161 atccatgttg gtgaagaaat cttggtcgaa cgtctagccg gccgatttat ctgcagcaat
    11221 tgtggagcaa cttaccataa aattttcaac cctacaaaag ttgaagacac atgtgatcgt
    11281 tgcggtgggc atgaattcta tcaaagagaa gatgataaac ctgagacggt taaaaatcgt
    11341 ctggctatca acatcaaaaa tagtgaacca atcttggctt attataaaga acaaggtttg
    11401 ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat ttgcagatgt acaaaaaatc
    11461 attgaaaaat agcacgattg cccgtagccg acttgcatta tgcagttact cttgtcaagt
    11521 tcaataaatt atgatagaat atgtcagtcc gacttctaga gaatcctcta gatgatccat
    11581 tttgagaatt ttgaggtggg aactgcagtt tccgccattt tatcgtgtga aaatgcgaaa
    11641 caagtacaag gaggtactgt gcgtggcaaa agaagatatg attgaagtcg aaggtacagt
    11701 cgtcgaaact ttgccgaatg caatgtttaa agttgaacta gaaaacggac accaagttct
    11761 tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt atcttacctg gtgacaaagt
    11821 aacggttgaa ttgtctccat acgatcttaa ccgtggccgt attacttatc gctttaaata
    11881 attgtactcc gtaaaactac aggaggtatt atcatgaaag taagaccatc agtaaaacca
    11941 atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg ttatggtgat ttgcccagca
    12001 aatccaaaac ataaacaacg tcaaggataa ttggaggtgc tataaatatg gctcgtattg
    12061 caggagtaga tattcctcgt gataaacgtg tagtagtttc acttacttat atctatggta
    12121 ttggtaacac tactgctaaa aagatcttag ctgatgctgg tgtatctgaa gatgttcgcg
    12181 ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc agaagtagat aaattaaaag
    12241 ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa acgtttgatg gaaatcggtt
    12301 cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg tggacaaaac acgaaaaaca
    12361 atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg caagaaaaaa taattcttaa
    12421 gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa tcgtaaacgc cgtgtgaaga
    12481 agaatataga agcaggtatt gcacatatcc attctacatt caataacact atcatcatga
    12541 tcactgatac tcacggaaat gcattagcgt ggtcatcagc tggttcatta ggtttcaaag
    12601 gaagcaaaaa atcaactcct tttgccgctc aaatggcagc agaaactgct acaaaagcag
    12661 caatggaaca cggattaaag actgttgaag taacagttaa aggacctggt tctggacgtg
    12721 aagcagcaat tcgttcatta caagctacag gtttagaagt gactgcaatt cgtgacgtga
    12781 ctccagttcc tcataatggt gcccgccctc caaaacgccg tcgtgtttaa tgagtgccgc
    12841 tcattttgag ttttatcagt aacgtcattg aacaagacac ttttcgtttt gaaaggggta
    12901 aagataagaa tgattgagtt cgaaaaacca agaatcgcaa aaattgatga agaaaaagat
    12961 tatggcaagt tcatcgttga acctcttgaa agagggtatg ggactacttt agggaattcc
    13021 ctacgtcgta ttttattatc ttctttgcca ggtgctgcca tcactaatat tcaaatcgat
    13081 ggtgtactgc atgaattctc taccatcaaa ggtgttagag aagacgtcac tcaaatcatc
    13141 ttgaacatta aaggcttagc gctgaaaatg tatggccaag aagaaaaaac ccttgaaatc
    13201 gatattaccg gacctgctac agtaactgct ggcgatatta tcgttgatag tgatgtagaa
    13261 attcttaaca aagatatgta catctgtagt gtctctgaag gagctacgtt ccatgctcgc
    13321 ttaacagtga aacctggacg tggttatgtt caagcagatg aaaataaaaa agaagatatg
    13381 ccaattggcg ttcttccagt cgattcaatc tacacacctg ttcgtcgtgt gaactatcaa
    13441 gttgagaaca cacgtgttgg acaccgtgaa gattttgata aattgacgat ggagatttgg
    13501 acagatggtt caattgaacc gttggaagca atgagtttgt ctgcgaaaat catgactgaa
    13561 catttggata tttttgtgaa cttaactgat gaagcaaaaa atgctgaaat catggtcgaa
    13621 aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga tcgaagagtt agacttgtct
    13681 gttcgctctt acaattgttt gaaacgtgca ggaatcaata ctgtacaaga acttacaaat
    13741 aaatctgagc ctgaaatgat caaagtccgt aacttaggac gtaaatcatt agaagaagtt
    13801 aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag aagattaaaa ccttttacga
    13861 aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca catctagcca acgtaaagcg
    13921 atgttgcgtg atttaacaac tgatttatta atcaacgaac gtattgtgac tactgaagct
    13981 cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca ctttaggtaa acgcggagat
    14041 ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg aagttgccag cgtgcgtgaa
    14101 gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac tgttcaacga tattgcacct
    14161 cgttacgcag aacgtcaagg cggatacaca cgcattttga agacagaacc aagacgcggt
    14221 gatggtgcac caatggttgt cttagaactt gtctaaaatc acactaataa catcacttta
    14281 tagatgattc ttttagagtg ttatgatgtt ggaagatatt tttccgagtc tagctcagcg
    14341 ctgctcctca agcgaatatc gcttggggag taagcactca tcataaagtg tttttttata
    14401 ccttcaatta cctgtcatga cagatatgtt tgaaggcata aaaaaacatt ttaagcgaga
    14461 aataattgaa aattttgtct ttttttgata gtgttagtag gaactttagg atgaagaagg
    14521 agtaatcatg gagccaatta ttgaattaga aaagataaat tataaatacc agccagatga
    14581 tcttcgcccc gcattgaaag atgtctcctt tacaattgat aaaggtgagt ggatcgctat
    14641 tattggacac aatggttcag gaaaatcgac acttgccaaa acgatcaatg gattgcttct
    14701 gccagaatca gggatcgtga aagtgggaaa tcaaatattg gatgaagaaa atatttggac
    14761 gatccgacag atggttggaa tgatttttca aaatccagac aatcagtttg ttggttcgac
    14821 agtagaagat gacgttgctt ttggtcttga aaatcaaggg attcccagag aagaaatgct
    14881 agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct gagtttgctt cacgcgagcc
    14941 tgctcgttta tcaggagggc aaaaacaacg tgttgccatt gccggcgtgg ttgccttacg
    15001 acctgatatc attattttag atgaagcaac cagtatgcta gatccagaag gtcgtgaaga
    15061 agtgatctct acgatcaaga agatcaaaga agaaagtcaa ttgaccgtta tatctatcac
    15121 tcatgatatt gatgaagcag ctaatgccaa tcggatacta gtgatgagac aaggcgaact
    15181 tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt cctgaactga tcgacttagg
    15241 tctagatcta ccatttcctg aaaaactgaa gagcgcactg aaagaacgtg gggttgatgt
    15301 gcccagtgaa tatatgacag aagaaaggat ggtggattgg ttatggacat ccgttttgaa
    15361 caagtagact tcacttatca gccgaataca ccatttgaac aaagagcctt atttgacatc
    15421 aatatgacga tcaaagaaaa cagttatact gcattagtag ggcataccgg aagcgggaaa
    15481 tcaaccttac ttcagcattt gaatgcgctt gttaagccaa caagcggtac agtccatatt
    15541 ggagaacgag atattcagcc ggatacggat aataagaatt taaagcccat ccgaaaaaaa
    15601 gttggcatcg tctttcagtt tccagaagca cagctattcg aagaaacggt agcaaaagac
    15661 attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag aagcactagt cctagcaaaa
    15721 gaaacattag aacaagttgg gctggatgaa agctatttgg aacgttcgcc atttgaactt
    15781 tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc ttgccatgag gccggaagta
    15841 cttgtattgg atgaaccaac ggcaggactt gatccccagg gcagaaaaga aatgatggag
    15901 atgttttggc gtcttcataa ggaacagcag atcactattg tattagttac gcatttaatg
    15961 gatgatgtag ccaattttgc agattatgtc tatgtgttgg aaaagggacg aatcgttaat
    16021 agcggggagc cgcaagaggt ctttcaaaac attgaatggc taaaagaaaa acaactaggc
    16081 gtacctactg ccaccgagtt tgcagaagaa ttaatggcta aagggatgaa ctttgctacc
    16141 ttgcctttga cagctgaaga attagcagac gctatcgtcc attatgctgg aggaaatgcc
    16201 catgatgaat aaattgatat ttggtcgtta tatgcctgga gattccttga tccatcgatt
    16261 ggatccccga gctaaattat tagccagttt ttattttatc ggaatcattt ttttagcaaa
    16321 taattgggaa acgtaccttt tgttggcatt tttcacatta ctttctgtct tcttgtcaaa
    16381 agtagatttg aaatttttta tccgaggcat tcgcccattg atctggctaa tcctcttcac
    16441 tgtagctttg caggtcttgt ttacaagtgg cggagaaact tattggagtt ggggtgtttt
    16501 ccatatcact gaatttggtg tgatgaacgg cctctttatt ttctgtcgat tcgtgttgat
    16561 catttttatg tcgactctac tcacgcttac tacaccgcct ttggaattat cggatgcgat
    16621 tgagtacttg cttcgtccat tgaaagccgt acggttccca gtccacgaaa tttcattgat
    16681 gctgtcgatt gctcttcgtt ttgtgcctac attgatggac gaaacagaaa agatcatgaa
    16741 cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac ctagttcaga aaatgaaagc
    16801 agtcattcct ttgctgattc ctttatttgt cagcagcttc aaccgtgcgg aagatcttgc
    16861 gactgctatg gaagcaagag gctacaaagg tggggaagga agaacgaaat atcgtgtatt
    16921 gcattggcat aatagagaca cgatggttat cattgctttt gtattattaa ctgtgctact
    16981 gatcttttta agaggataaa cgttcagtgg gccgctatct attcttttct tgctgatctg
    17041 tttgcaagaa aggatgcaag cagtgtatga caagaaatat tagggtctgt ggcagactaa
    17101 ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga aaaaatttta tagaaaaatc
    17161 attattccag ataacctttt aggaacaaag gagagaaaaa catggtacgt tacaaagcaa
    17221 tcattgcgta cgacggaaca aattttaatg gattccaaaa acagccaaat gggcgtactg
    17281 ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa tggaaaagaa atcacggtct
    17341 ttggttcggg aaggacagat gccggagtac atgcgatcgg gcaagtaatc cattttgatt
    17401 atccagagga gcgtccgtta gaacgaatgc gtttcgcgct agatacccag tcaccagagg
    17461 atattgctgt aagacaagta gaaattgttt cagaagactt ccacgcaaga tatttggtca
    17521 aagaaaagac gtatcaattt cgagtggata tagggaaacc acgcagtcct tttcgacgcc
    17581 attatgcaag ttattttcct tacccacttg atttggaaaa aattcagcgg gcgcttcctg
    17641 atttattagg gactcacgat ttcacctctt tttgtgcatc aggaagctcg atagaggata
    17701 aaatacgtac gatctacgag gctaagatgg aggtaaatga aacaaaagac gaattgttgt
    17761 ttacatttag gggaaatggc ttcttgtata agatgatacg gatcttagta ggaacgcttt
    17821 tgaaaattgg caatggccga ttgcctgaag atagtatccc agatattatt gcgaaaaaag
    17881 atcgtaatgc agcaggacct acagcacacc ctgaaggact ttatttgtat gaagtgaaat
    17941 atgaataaga ggttatgata gaagtggtca gcttcaagaa ataagaaggt atttacgaaa
    18001 attacttctc aaaattttgc aaaatttcgg cttattttcg aagaagctac ttctggaaca
    18061 ccgtttaaga aagaggtcgt gagcctgtca tttaactctg agtcacaaag agcaatttca
    18121 aaaaacagct tctcatcttt gatgaatatc gtgacttgtg gacgaggcgt tgataagtga
    18181 atcatctttt tgttacctat tttttctgta accaaaaaac gatggaacgt tgttttgatt
    18241 gcccttggtg cggacagtct gaacaggttc catcgtttct ttattttcta cgtcttcttc
    18301 tgtgtgtgtt tcgtggttgt tcttctattg ctggatcaat cgtgccataa aaggggcgat
    18361 tgcggtcaag gtaccagaca taaagaataa acaatgcaat acataggaca aaaaggattg
    18421 cacctatcgt aaatccttca ggcaagtaac tgaaagtgac agtatgttta cctgctggaa
    18481 cttttatcat caagaatgcg tctttgaaat tgctgacctt gactggtttc ccatcaattt
    18541 tagctttcca tcctttgtca taaggaatag ttgtcaccaa catggaatct tcagcggtat
    18601 ttacagtccc agaagctttt ctgccagtgg cttccatttc gacacctttt tctttgatct
    18661 gattcattgc ggcttgatag gctacaacat ctagaccaac gactttaggc tccatgaaac
    18721 ttatttcttt cgtcccataa aaacttgcag taaatgtcac tgttgtctct tgaggataat
    18781 agccaagatc atagtattga ccagaaatgt tgatttgcgt ttttcgcgat gtaccattga
    18841 cagtgatcgt cgcactagaa ctttctaatt gcgcaaaatt ggttgggaac aaacttaagt
    18901 aagcttgtgt atttgcaggc acatggactt cccaagtgag ctcttttggc tggttatctg
    18961 ctgtttctga gtaagtgacg ccagtcgttg tctttgtgat cgtcgtattc aactgattct
    19021 tcaaagtagg tgtatagaat gtaaaatact gttcatttag accagacaat gtattgatca
    19081 agtttgtctg actggtcaaa ttatctgtca ctggctgctc aacatcatta acggacaacg
    19141 gtgcagtaaa tgctagtggc aatgcattga cattctgata cagtgtatag tcgccgcttt
    19201 gaccagcttg gcggaagcca tatttactga acgtgctttc gtttttagaa atgttatatt
    19261 tgattcctgt aaacccatcc atcagcaaag tattattgtt ataacggata ttcaaatttg
    19321 tccctcttga acggaaacct aatttatcca ggtaagagga agaattacga tttcttatag
    19381 aagaaaacaa actgatacca ctgtaaccat agttgatcga atcgtttgca gatactggat
    19441 ccagattttc caagcggtaa aacgagtcgg aatctttttt tgtttcattt acaagagctt
    19501 tcaagtcagg ataaggatca gtatataaac tgcgggaagc ataattccaa tcatcaagaa
    19561 tgccatggat catactgttc gtattgacca gtgcttcagc agacatgatc aataataaca
    19621 atatactcag atgattgaat gaaagttttt tatgctgata aaatcccaca gcaaaagcat
    19681 aaatccctaa gaaaaccact gtcaatacaa aggaagtcat cgtcacatag gtgtagcttc
    19741 cttcaggttt gatgccccat gccaccgcaa agagagcagc tacgaccaaa atgattccta
    19801 acaacagccc accatcgtct tgttctatct tttcccatcc ttttcctgca agcaggagca
    19861 ctaagaaaga gaacagatag gcataacgga ataaaaacat attcggtgca tgcataccat
    19921 gccagaacaa attcaatgga acaagataaa aacttgcgat aagtatggcg aaaagactgc
    19981 cgtacaaaaa tttatttttc aacggtactt tccgactggc aaaataaaac agacagagga
    20041 ctaatggtag cagacctgcg taaacgaacg gaatcgatcc gtattttgtc gtgtcataaa
    20101 caccaatcat atttttcatg attagatcca gataagaagt cgcttctgtt ttcaacgtag
    20161 tgatctgact cagtgcctcg ccatttgacc gaagatccag cactgctggc aatacaatga
    20221 tcattgaagc gccgcctgcc agtaaagaag tgataccata aggaacaatc gtttttttat
    20281 attctgacca gtttgtcaat agacgggcga taaaatataa gaaagaaaat acgccaatca
    20341 taaaacccat ataaaaactg gataaaaaca agagtaaata actgataaat aaaagtttcg
    20401 gccgtttaag atccataaca cgatgaatcc ccaaaatcac taatggaaga taaacaaatg
    20461 catccagcca catgatgatc tccgaatgtg cagtgataaa tgagatcaat gcgtaactga
    20521 cgctaagtgc aacatgcccc cagcgaggaa tcttgaacgt acgtgcaaga aaccaaaaag
    20581 ccaatccggc acagccgacc ttaagcaaag tcagaacata cagtgcatct ggcatcatct
    20641 ggtttggaaa aaagaacacc agtggcgtga agatcccgcc tagatagtaa gaaattaaag
    20701 ataaataatt caatcctaga gaagcattcc atgtataaaa aatactttgc tttccaagaa
    20761 gaacatttcg gaagctggca tgaaaattag aaaactgtga aaaggcatca cttgccaaaa
    20821 tacttctgct gcttccagga tagattccga tacttagata aacaagtgct aaaatcaaaa
    20881 acggaatcag gaaactagct gctgcgtagc gtccgttttc tctaaagaat gactttaaat
    20941 tttttttcat atttattttc caccaaattt ctatgaagtt gtattcagtt t
//
LOCUS       DAZUMV010000037        20963 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000037 DAZUMV010000000
VERSION     DAZUMV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.037"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..2228
                     /locus_tag="ACG8FK_001259"
     CDS             168..2228
                     /locus_tag="ACG8FK_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS5096875.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2311..2880
                     /locus_tag="ACG8FK_001260"
     CDS             2311..2880
                     /locus_tag="ACG8FK_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5096876.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3055..3861)
                     /locus_tag="ACG8FK_001261"
     CDS             complement(3055..3861)
                     /locus_tag="ACG8FK_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096877.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3999..5513
                     /locus_tag="ACG8FK_001262"
     CDS             3999..5513
                     /locus_tag="ACG8FK_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS5096878.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5916..6899)
                     /locus_tag="ACG8FK_001263"
     CDS             complement(5916..6899)
                     /locus_tag="ACG8FK_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS5096879.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6904..7551)
                     /locus_tag="ACG8FK_001264"
     CDS             complement(6904..7551)
                     /locus_tag="ACG8FK_001264"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5096880.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7636..8268)
                     /locus_tag="ACG8FK_001265"
     CDS             complement(7636..8268)
                     /locus_tag="ACG8FK_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5096881.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8284..8835)
                     /gene="coaC"
                     /locus_tag="ACG8FK_001266"
     CDS             complement(8284..8835)
                     /gene="coaC"
                     /locus_tag="ACG8FK_001266"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS5096882.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8832..9569)
                     /locus_tag="ACG8FK_001267"
     CDS             complement(8832..9569)
                     /locus_tag="ACG8FK_001267"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS5096883.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9566..10555)
                     /locus_tag="ACG8FK_001268"
     CDS             complement(9566..10555)
                     /locus_tag="ACG8FK_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS5096884.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10813..11355
                     /locus_tag="ACG8FK_001269"
     CDS             10813..11355
                     /locus_tag="ACG8FK_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS5096885.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11372..12457
                     /locus_tag="ACG8FK_001270"
     CDS             11372..12457
                     /locus_tag="ACG8FK_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096886.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12469..13275
                     /locus_tag="ACG8FK_001271"
     CDS             12469..13275
                     /locus_tag="ACG8FK_001271"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096887.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13276..14103
                     /locus_tag="ACG8FK_001272"
     CDS             13276..14103
                     /locus_tag="ACG8FK_001272"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096888.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14100..15173
                     /locus_tag="ACG8FK_001273"
     CDS             14100..15173
                     /locus_tag="ACG8FK_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5096889.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15601..18297
                     /locus_tag="ACG8FK_001274"
     CDS             15601..18297
                     /locus_tag="ACG8FK_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5096890.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEEDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTEGLLATANNGYLFL
                     DEVHRLSKENQEKLFVFIDTGNYRPVGENVNWHSAKVRFIFATTEKSEDYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADILQKDIIVSKEALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTREEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNEKFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKYENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKEYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLKHILSSEMKPVEFNRLIQELIKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKAEEPNYDMKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18362..18862
                     /locus_tag="ACG8FK_001275"
     CDS             18362..18862
                     /locus_tag="ACG8FK_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5096891.1"
                     /translation="MIKPMFLKVGTGIAYMKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDYEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQEIKMTILDEVHVVN
                     EEEDFF"
     gene            18874..19368
                     /locus_tag="ACG8FK_001276"
     CDS             18874..19368
                     /locus_tag="ACG8FK_001276"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5096892.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19390..20199
                     /locus_tag="ACG8FK_001277"
     CDS             19390..20199
                     /locus_tag="ACG8FK_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5096893.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20196..>20963
                     /locus_tag="ACG8FK_001278"
     CDS             20196..>20963
                     /locus_tag="ACG8FK_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5096894.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKI"
BASE COUNT         6705 a         3653 c         4384 g         6221 t
ORIGIN      
        1 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
       61 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
      121 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
      181 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
      241 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
      301 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
      361 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
      421 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
      481 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
      541 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
      601 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
      661 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
      721 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
      781 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
      841 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
      901 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
      961 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     1021 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     1081 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     1141 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     1201 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     1261 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     1321 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     1381 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     1441 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     1501 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     1561 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     1621 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     1681 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     1741 tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc agaaaaaaaa caacaaatgg
     1801 ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     1861 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     1921 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     1981 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     2041 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     2101 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     2161 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     2221 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     2281 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     2341 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     2401 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     2461 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     2521 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     2581 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     2641 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     2701 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     2761 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     2821 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     2881 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     2941 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     3001 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     3061 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     3121 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     3181 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     3241 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     3301 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     3361 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     3421 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     3481 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     3541 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     3601 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     3661 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     3721 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     3781 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     3841 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     3901 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     3961 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     4021 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     4081 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     4141 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     4201 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     4261 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     4321 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     4381 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     4441 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     4501 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     4561 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     4621 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     4681 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     4741 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     4801 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     4861 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     4921 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     4981 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     5041 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     5101 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     5161 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     5221 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     5281 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     5341 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     5401 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     5461 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     5521 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     5581 gaacactgtt tagtgaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     5641 caagtaaaaa cagcttctta tgtttttaac ttggttggac ttattaccgc agatgcaagc
     5701 ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa agtaaagaac acttttgtct
     5761 cacgctctaa acacgtataa acggcggaag cagaagaaat tccttcggaa ataagctgaa
     5821 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg
     5881 cttctgtccc agcctctttt ttgatcagca tgattttatt cagttatctt caaaatgatt
     5941 tttccgatgt ttttgtttgc ttccatatat tcatgcgctt gtttgactgc gctgaattgg
     6001 aatgtacggt caatgattgg ccgaatcttg ccattttcta gaaaaggccc agctttttgc
     6061 ataaaagttt gtgcgagctc tgctttatac tgatcacttc taggtgtcaa taatgtacca
     6121 agcagttgga cacgtttccc aatcaataca ccaaggtcta tctcaggcac gatggtccca
     6181 cctagcatac caatcaagac ccaacgtcca tcaacggcta tagaagctaa attcttttcc
     6241 cagtaagaag cgccgatgaa atccaaaata acatcgactc cccgaccttc agtgattttt
     6301 cgtacctctt cttgaaagtc ttgtttttta tagttgatca attcatctgc accaagttct
     6361 ttacaaaagg cgagtttttc ttctgatcct gcagtaacga tgattttcgc gtgagaaagc
     6421 tgtctagcta attggatcgc tgctgttccc acaccacttg ctcctgcatg gatcaagaca
     6481 gtttcgcctt cttgtaattt tccaagccag taaagtgttt gataagcagt caaaaaaact
     6541 tctgagattc cagcagcaga aacatagtct agcgtatcag ggagaaggat tgcacgatcg
     6601 gcaggcatca cagcatattc ggcataacca cctagattga ctaatccgta tacttttgtc
     6661 ccaggagtaa aagtggagat atcactttta ttctcaacta cctctccagc tacttcgact
     6721 cccaaaatag ggtagggagg tttcaaagag actgattctc ttttcataat atctgttcga
     6781 ttgacagctg ctgtatggac tttgatcagt aattcgcctt gttttggttt aggaacaggc
     6841 acttcaattt gttctaatac atctacagaa ccaggatgct tgattgaaat ggcacgcatg
     6901 gatctattcc tcctttgccc attcttggaa tgctgtattc aactctttgc taccttcaga
     6961 aagtactaga gtcgtacgag aaatatccgt ttcttccact agagcggcaa gtacagcagc
     7021 aactgtagag cgagtgatcg tttttgatgc cttctcatta ggcttagtta attgaatgga
     7081 agtaacttct tcatcgttag tcaatgtgac tggttgaaga atgacaaaat ctaagttagt
     7141 tcgtttcagc cattcatctg cataatgttt agtaatataa taatctgtca tggactcagg
     7201 ccattttgct ggttcatctg cataaacagc actgaccatc agatagcgag agacatgcgc
     7261 attttcagct gcgatcactg ttttgattgc gccatccaag tctacttgca gaagattctt
     7321 tccttgagag ccggcagcaa aaatcaatac atcgatgcct ttgaatgctt cggtcatttt
     7381 ttccacggac caagttaaat caaaagagac atacgaaaca ttctcttttt ttactaatgt
     7441 ttgggaagca acatctctca cccctgcaaa aatttcatgg gaacttggag ctaaatcttt
     7501 tatcaggtgt cggccaattt gtccgtttgc acctacaaca aaaattttca tttggatcat
     7561 cctttcgacg tcaaatcttt ccattcaata gtaacaccaa aagagaaaaa aatcagagat
     7621 cggctacgat gactatcagt tagtaagaga agggtgcttg aagtatctta acaagacatt
     7681 gcatactgct gcagtcgcaa gacctgcgac gattccttca ggtataccac ttgtaaaaat
     7741 aaccgctaga atagctgaat aaacagcatt taaatttgtt ccgatgactt tggcataatc
     7801 ttgctggaat aaaaagtaga tcatattcat cactaaaatc gtattggtca atgaaccgga
     7861 aagtccagct aagaataaag aaacggaatc tgctttttgt ttcattagtt tgcgcagacc
     7921 tttataaacg aaataaggaa ccaccccaat caagatgcga ggaataaacg cgatcagcaa
     7981 ggctttccag cttccatgtt ctgttccgat gactgggata aacggagaaa agacaaaaga
     8041 caaaggtgtt tggataactg tgctacggat catgctgatc agtccaaatg taccgccaag
     8101 aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg attacaggaa tatgcatggt
     8161 tgttgcattg attggaccga tagggataaa acctaaaggc gtaacagcta ataaaatcaa
     8221 aatagctaaa aacatcgcag ttaatgtaaa gttttttgta tttttcataa gtactccttt
     8281 tttctaagca tttctttcat taagagtcag catgacctgt tcgatgatcg tctgaatatc
     8341 agctaaggca ccttttccaa aatctccaca agctaaaaga gattctcttg gttcaatttc
     8401 ctgatagccg acagatctca atgtttccag attacgctga gtgatcgggt tttgatacat
     8461 attcgtgttc atggcagggg caatcaattt tggtgttccc actggaagcg ccattgcgac
     8521 tgttgaaaga agatcgtctg caatgccgtt tgctagcttg ccgataatgt ttgctgtagc
     8581 aggcgcgatg agaaacaagt cagcttgttt tgctagttcg atatgattga ttacctgcgg
     8641 atttgattct tgcatgacgt ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa
     8701 tggcgtgata aaatgtgtgc tgcttttaga cataatcaca tctacctggg cacctaattt
     8761 gacgaattga cttgtaagat cagcgctttt gtaagcagaa atacttccag taacgcctag
     8821 taagatcttt ttcattttga ttcctccagt ctaattttct cagtaatcaa tgcagcaatc
     8881 tcagatttac tttgagcttc ttccactgtt ccatctttag ataagagata gccgtggtgt
     8941 gtctcgccat ggatttccgt caaatcattt gcgaaaataa aatcagtttt gttatttatc
     9001 agactatttt ttgcgactgc cagaagagat tcctttggta catctactaa tagtttgaaa
     9061 ccaatgattt ttgcttttgg ctgccattca cgtagataag aaatgatttt tggtgttttc
     9121 ttcaaaacga gcacaagatg ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc
     9181 tgttcatttt ccgcaaacca attgtccagt tcttcttgcg ttgaagaagt gggtaggttt
     9241 tttgccagct gttcttctga aaaactaaaa gcaggagtaa aatcactgac tgccatactg
     9301 tggataatcg catcgtagtg ttcttttttc gttagttctt ctaattgtaa aaataagtcc
     9361 agtgcagatt cgatcgtata aatcgttata ttggagtgtc ttttcggttt cagtgcttgt
     9421 ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag ctaaataatc agctaatgct
     9481 tgccctaatc gtcctgtcga atgatttgta atagcacgta cttgatcgat tttttcagtc
     9541 gttcctccag ctgtgattag aatcttcatt tatggtcacc tacatttact agtgttcttc
     9601 cttggtgtgt tccagcaagt agtttagatg ctgtttcagg caattgatcc agagtaatct
     9661 cttggacgac cagttcatct gcaatctgca aatccgccat ctgttgccat aaagaaagac
     9721 gttgatctat tggtacattg acagaatcaa ttccaatcaa ctggatgttt cgcagaataa
     9781 aaggcaagac agtcgcgttt agtttgattc ccccagcatt gccacataaa gctactgcac
     9841 cgttataaga gatcaatgga agaagactgc ttagctgctc gccacctact gtgtcgatga
     9901 catagtcgat ttgctgtttt cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc
     9961 gtacgacttg caaagcacct agacttttta gccatgtgac agactcttct tttctggaaa
    10021 aggcaatgat tgactggaat cctagtttat gtaacagggc tatcgctgta ctgcctacac
    10081 cgccagaagc accagtaacg acaacttttt tatcggaggt cataccttga cggattaaag
    10141 cgttcacaca caacatagca gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg
    10201 tcatattttt tggtagagga actaaccagt ctcctggtac tttttgatat tggctataac
    10261 cgcctggatg ggtgacgcca agcccaaaac cagtagcaag tacaagatct tcttttgaaa
    10321 aacgtggatt gttactttgg acgacagttc cggataaatc aatccctgga gtcatcggat
    10381 atgttcgtat cacctgacca gactcgctca ttgccaatgc atctttataa ttgacatcag
    10441 aataggcaat ttttaccaag acttcatttt cagccaactc agtaaatggc agatcactga
    10501 ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga tcgaaaagcg ttcattcgtg
    10561 agacactcct tttaaaattt tcttttatca taccataaag cctactcttt tattcttgtt
    10621 cttccgtaaa tttagacaaa aattaggtga aaaataaaga atcaaacaac tagaatttat
    10681 tccaaaagat aaagaaatat tcagcaaaaa aaattttaca aagggttgcc tttttcaata
    10741 aatttcgtat actaatacga gtggtagtaa acaggaagtt tactattaga aaacttcgat
    10801 tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg
    10861 acacaagaag aactaggcga acgcacagat ttgtcaaaag ggtatatttc ccagttggaa
    10921 cgagatttaa gttcaccatc aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg
    10981 actccagaag aattttttca tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa
    11041 gatcatacgg tttatttcga tgaagaaaac gggtatgaat tggaatggct cgtggctgat
    11101 tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg aaggtgcagg agaatataag
    11161 acgtttgaac cttcattatc tgaaacgttt atttttgtat tagaagggga attagagtta
    11221 agcttaggag agacggtcta tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca
    11281 aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg
    11341 caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa ccaatcattt catttgatca
    11401 agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa gtaagttttg agatcgaaca
    11461 aggaaaattt tatacactgc ttggtccttc cggctgtggt aaaacaacga tcctacgtat
    11521 tatagctgga ttcaccgaag ctacagaagg ggacatctat tttgaaggga aacggttgaa
    11581 tgatttgcca gctaataagc gacaagtaaa tacagtcttc caagattatg cactttttcc
    11641 tcatatgaat gtttttgata acgttgcttt tggattgaag atcaagaaaa tgtccaaatc
    11701 agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa
    11761 acgggagatt agtgaaatgt ctggtggtca aagacagcgt gtagcgattg cacgagcaat
    11821 tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta tcggcgcttg atttgaaact
    11881 acgtacagat atgcagtatg aactgcggga tctgcaacaa cgattaggaa tcacgtttat
    11941 ttttgttact catgaccaag aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa
    12001 taaagggaaa atcgtccaaa gtggtacacc cgtagatatt tatgatgagc caatcaatca
    12061 tttcgttgct gattttgttg gggagagcaa tatcgtggac ggtgtaatga tcgaagataa
    12121 tctagttgag tttgttggta agcaatttga atgtgtcgat ggcgggatgc gtctaaatga
    12181 accagttgaa gttgttttac gcccagagga tttgacgatc acgacaccag ataaaggaaa
    12241 actagtcgta acggtcgata ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta
    12301 tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa
    12361 agtaggattg gcctttgagc cagaagatat ccatgtcatg cgtttcaacg agtctgaaga
    12421 agaattcgat gcacgtcttg acagctacga agaatagggg ggacaagaat gaaaacaatg
    12481 cgcaggatct attccgtccc ttatattttg tggctcggtt tattcgtgat cgcgcctgta
    12541 ttaatgatca tttatcaatc tttttttgat atgaatgggc aattcacgct gaataattat
    12601 ctatcttatt ttacgtcggg gacatcgctt tctatgacga tcaattctgt atggtatgca
    12661 tttttgatca cgtttttcac gctgatcatc agttacccga ctgcttattt tttaacaaaa
    12721 ctgaagcaca agcaattatg gctgatgctg attattttgc ctacatgggt gaatctgctg
    12781 cttaaagctt acgcatttat tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt
    12841 gattttatcg ggatcgggcc gaaacagata ttattcacag acttcagttt tctgttcgtg
    12901 gcaacgtata tcgagattcc atttatgatt atgccaattt tcaatgcatt ggaagagatg
    12961 aatccttctt tgatcagtgc tagtagagat ttaggcgcga atagctttga gaccttccga
    13021 cgagtgattt ttccgttatc attaaatggt gtgaaaagtg gtgtccaagc agtatttatt
    13081 ccttctcttt ctttattcat gctgactcgt ctgattggcg gaaatcgggt gatcacttta
    13141 ggaacggcta tcgaacagca tttcttggtt acacaaaact gggggatggg ttctacaatc
    13201 ggtgtgattt taatcattgc tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa
    13261 ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt
    13321 ttattcttat atgcaccgat cttctatctg attttttatt ccttcaatga aggaggaacg
    13381 atgaacagct ttacaggatt tacttgggaa aattatgaag ctgtttttga agatacacgt
    13441 ttgatcatta tcgtattgaa tacgttcatg ctggctttct tatctgcatt gatcgccacg
    13501 atcatcggga catttggggc tatgggaatc tattacacga aaaaacgacg tgcacggaca
    13561 gcgttgttga gcatgaataa catcttatta gtctctcctg atgttattat cggtgctagt
    13621 ttcttgattt tctttacact actaggattc ggattaggtt ttcagagtgt attgctttcc
    13681 catattgctt tcagcattcc aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg
    13741 aacgattcaa tggtcgatgc ggctagagac cttggtgcta ataatttcca agtagtcaaa
    13801 aatatcattt tgccttatct ttctccagga atcatagctg gatattttat ggcatttacc
    13861 tattctcttg atgattttgc ggttacattt ttcgtcacag gaaatggctt tacgacgctc
    13921 tcagtagaga tctattctcg tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt
    13981 gcgttagttt tcttgttctc catgatattg gtcattggat actactttat tagtaaagaa
    14041 aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg agggggtggc acggctgcga
    14101 tgaaaaaatt acaatctctg tttttaggta ttctcatcat tattgcggtg ctgctgcttg
    14161 gtgttcgtca acttgaaaaa acaagtggca tgagtggagc aaaggtactt accatctata
    14221 actggggaga ttatatcgat cctagtttga tcacgaaatt tgaaaaagaa tatggataca
    14281 aagtgaatta tgaaacattt gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg
    14341 ggacaaacta cgacatcgct ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa
    14401 aactagtact tccattagat cattcgaaga tcaaaggatt aaataatatt gatccacgat
    14461 ttttagactt ggattttgat ccagataatc agtattcgat tccatatttt tggggaactt
    14521 tagggatcgt atataacgat aaattctttg atgcaaatca aattaagcat tgggatgatt
    14581 tgtggaagcc ggaattaaaa gacagtttga tgctgattga cggtgcacga gaagttatgg
    14641 ggctttcttt gaatagttta ggctattctt tgaatagtaa gaatatggca cagctgacag
    14701 aagcagcaaa taaattgaat catttaacac caaatgtcaa agcaatcgtg gcagatgaga
    14761 tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt gacattttct ggcgaagctg
    14821 ccgatatgat gtgggaaaat gaacatttac attatgtgat cccaccagaa ggatccaatc
    14881 tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt
    14941 tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa tgcagagtat attggttatt
    15001 ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga catttcgtcg gacgaacaat
    15061 tctatccatc tgatgacacg atctcccatc ttgaagtcta caaagatcta ggatcgaaat
    15121 acctaggcat ctataatgac ctattcttag aattcaagat gtataggaga taatggcaaa
    15181 actcagtaag aatggggact tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat
    15241 ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc gctttttttg tgttatttta
    15301 ttttttctac cacgtttttc taccagagaa aagtggacaa aaccattgta aaatagggcc
    15361 cgagacgggc tagtaaatct aagattgaaa aagttacaga aggataaagt aattggaaac
    15421 tatgcaaata tacttaaccc ttctgtaatc tattaataaa actaatatat gaatccagtt
    15481 caatatttgt agctatgaaa aaacataaaa atcatattag ataaaaacta aattggtaga
    15541 taaattattt tgtcagatat ctgaggcaaa tattcgacta tttgtgtttc aaagtaaaaa
    15601 ttggaataca aatatagaat agacatattg cagccgatag tttattatgc ttttagacac
    15661 attaaactgg aggaaaaaat gaaaaagagc gctaaagatc gagtttttga tgagctgata
    15721 cgactaacag ggaatatgtc ttctgtcact gccgagaaaa tttcgaagaa tctaggtatc
    15781 tctcgtcaga atgccagcca ttatttgacg agattagtgg aagataaaaa ggtcgagaaa
    15841 ttaccgggaa agcctgtttt gtggaagcct ttagatgaat atgcggttgt agataatacg
    15901 gagcaaataa acgaagcctt tcattctgtg gtgggtcatg acggaagtct gagagaggtc
    15961 attcaaaaat gtattgcagc tgtcaaatat ccgcctaacg gactcagtgt attaataaat
    16021 ggagccacgg gggttggaaa aagtttttta gccacaaaaa tttttgaata cgctatccac
    16081 gagcaaatta ttgaagaaga tgcaccattt gctattttaa attgtgccga ttatgcggac
    16141 aatcccgaac tgctttcggc aacactgttt ggatacaaga aaggggcctt tacgggggct
    16201 gagaaggata cagaggggtt attggcaaca gccaacaatg gctatctttt tcttgatgag
    16261 gtccatcgtt tgtcgaaaga aaatcaagaa aagctctttg tttttattga tactggaaat
    16321 tatcgtccag tcggtgaaaa tgtaaactgg cattctgcta aggttcgttt tatcttcgcc
    16381 acgacggaaa aaagtgaaga ttatttatta gacacctttg atcgacgtat tcaaatcagt
    16441 gttatgttgc caacttttga agatcgtccc attcgagaac ggttagagct gattcaatta
    16501 ttttttcaaa atgaagcgga tattttacaa aaagatatta ttgtttcaaa agaagctttg
    16561 tctctaatgt tatctcatcc ctttagtggg aatattggta agctgaagaa tatcattaaa
    16621 atcagttgtg cggatgtgta cagtcgaaca cgggaagagc cattaacgat tgggaaaaat
    16681 gagatcttga ttcagttaaa tatgctggaa tccgaagtag aaagcttgcc gattgctagt
    16741 ttattgataa agaaaaatca aaagataaaa acggatcgaa tcaaaccgtt tgattatgat
    16801 tcgattttgg aaaaaattac tcagctgata aatgaaggta atttcaatga aaagttttct
    16861 tcgataaaga atgaagtaca gcatttggtg catcacattg ataaggattc ttttttatca
    16921 atgagtagaa aattgatgat tcagatgttt agaaaggtct gggtatccgt tttaggaaaa
    16981 acgtacggat tgacgacgag tggacctgtt gcagaaatta ttagcagatc ctatgtcatt
    17041 tccccaagct ataagacaga cttaaccggg ataaatcgta cattagctgc aaatctacct
    17101 cggacgactt acttatgtaa tcagttcttg gagcatttac caccgctctc agaaaagtac
    17161 gagaactttc taagactgct tattgtcgtg actttatctg attatgtaga tgaatccatc
    17221 gagctgaagg gcctgttgct tgctcacgga gatagtacgg ctagcagcat tcaagcagta
    17281 gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg atatgccagt agagagtaat
    17341 gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac ggcaagtgaa tacagatagt
    17401 ctagtgctga tcgtagatat gggctcttta aatcaaatct acacacagat caagaacgat
    17461 ttaaaaggag aattacttct tctgaataat ttgacgacct ccattgcact tgatattggc
    17521 ctgaagatga cgatgaatgc gccatttaaa cagattgcgg aggatgcagc agacaagtat
    17581 tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta gcaacatcat catttcctgc
    17641 atgtcgggat tgggtatctc ggataaactg aaggatgttt ttcgtcatca tttatcggat
    17701 gagtccatcg aagttttcac gaaggaatac agagaactac gtgaattaat cgaccaaaat
    17761 gatgaaactt attttgacaa gacaaaattg gtcatcacga cttcggatct tccacgttcc
    17821 ttttcaattc ctaatatcaa tgtatatgat attttagatg gtgatggagc agccaatttg
    17881 aagcatattt tgagttctga gatgaagccg gtagaattta accgtttgat tcaggagctg
    17941 ataaaattct tttcattgga aggaatcgca gaccgtttga actttctgaa cccaacaatc
    18001 gtgatgagtg aggtggaaac agtcatttct aaatatgaaa attactatca tttcacttta
    18061 aatggccgcg taaaactaaa tctttatatg catacggcat tgatgatgga acgtttattc
    18121 ttggcgcgga cagaaaaagc agaagaacca aattatgata tgaaaccaga tgaagatgaa
    18181 ttttattatg tttcgagaag tatttttcaa tcaatggaga tgaaatataa ttttaaagtc
    18241 aatgcttatg agctttcgct gctctatgaa ttattaggac cgtatattca aaaataaagt
    18301 gtctaaaaaa gtgtctaatc tgattttttc aggttagaca ctttttttta tgtgaattaa
    18361 cgtgattaaa ccaatgtttt taaaagttgg cacaggaatt gcttatatga aagtgaacag
    18421 aaagaaagga attaaccata tgaagaaaat tctaatagcg actcacggtc acttagcttc
    18481 aggctttctt agcagtattc aattactgac ggggaaggtt caagagatta cggtgattaa
    18541 tgcttatgtg gatgagtgtg attatgaaca ggagttgaat gtatttgtaa accaggtgaa
    18601 cgagcaggat cagatttttg tttttaccga tttatttggc ggaagtgtca atcagaagat
    18661 taccaaaacc tttttggaga aagagattgc tgtaactatt ttgtctggct ttaatctgcc
    18721 aattctgctc gaaatagtat tggcaaccaa ggaattaaca aaggaggata ttcaggaact
    18781 ggtaaataaa tgccaacagg aaataaagat gaccattctt gatgaggtac atgtagtaaa
    18841 tgaagaggaa gatttttttt aggaggaaga aaaatgatta aagcagtgcg aattgatgaa
    18901 cgattaattc atggacaagt agcgatgact tggacaaaga gtctaaattt aaccgggtta
    18961 gtagtagcca gcgatgaggc ggctaccaac gatattcaaa agatgacatt gaagatggct
    19021 gcaccatcga atgtcaaagt cattattaaa acagttgatg acgcgattca tttgttaaaa
    19081 gatccgcggg cgaaggacat gcgcttgatg gttttagtcc ctaatgtcaa agatgcggta
    19141 aaagttgcga aagctttccc aacggaaatc gaattaatga acgtggggaa tgctgggaaa
    19201 atgacaccgc catcggaaga taagaaaacc ttatcaaagg aagtgatgtt aacgacgtca
    19261 gaaattcaag acttaaaaga attagttgaa ctttatccag ataccgtttt tcagcctaca
    19321 ccggcgatgg aaaaacgttc agctatctct attttgagtg atttttagaa aaaaggaagg
    19381 agaaaaatta tgtatgatgc atttgtagtc gctttggccg tttttattgg agttgctgga
    19441 catgaaatgt tcgggatgtc catgctgagt cgtccgattg ttgttgctcc gttagcagga
    19501 gcgttgatgg gggatttgca gacggggttg attgtcgggg cgtcactaga agccattttt
    19561 atgggtgtcg tcaatatcgg aatctctagt acggcggagc cttctttggc tgctgggttg
    19621 gctacagcgt tcgcaatcaa aatgggagga aacgtggatg cgattattcc cattgtcttt
    19681 ccactagcag tattaggatt acagttaatg aatatgattt tttcttttgt atgtggaccg
    19741 atggctgcta aatttttcaa atttgcaaaa tctggtgatc aaaaaggaat catcaatctg
    19801 cacttcgtta tgtggtttgt ccactatgcg ctttatgctc ttattccatt ctttgccgtt
    19861 ttatttggag cagatgttgt caaaagcgtc ttggataata ttccagaggt catcatgaac
    19921 ggattaacag tcgctggaaa tctgctgccg gcagttggga tggctatgct gttacggatg
    19981 ctttggagta aaaatattgc tgtgtggttt ttctttgggg ccgttgttat ggcgtatctc
    20041 gagttaccat tgattgcaat tgccgcagtt ggaacgatta ttgcaattgt tatagcccaa
    20101 agagatctac aactaaaaaa tatatctgtt catgcagttc aaagcgatgc tgctgtaaat
    20161 gcaacgcttg accaagaaga ggaggatttc tttcaatgaa attagctgat ggaatttcta
    20221 aagatgagaa aaaattggtt cggaagatgt tttggcgctc gatgactatg tatgcttcgg
    20281 tgaatccggt gacgatgggc ggcggtggtt tcgcctattc tatgatgccc ttcattaatc
    20341 atttctacaa aagtgaagaa gatcgagctg cggcgctgga acgtcataca cagtattttt
    20401 cgacgaccat tccttgcgcg tcctttgtca tggggattgc ggcatcgatg gagaaggaaa
    20461 attctgaaaa agctggtttt gacgtggaaa gtatcaacgc gatcaaaacg agtttgatgg
    20521 gaccattggc tggaattggc gattcattgt tctggggagt ttggcgtgtc gttaccgcgg
    20581 gactagcgat caacttagcc aatgccggaa atattttggc accaattatc tttttagtta
    20641 tgttcaatat acctaattat ttgatgcgat actatggcgg cgttttaggc tattcattag
    20701 gctctaaata tattgaaaag ctgtatgcga acgggttaat tgaaattctg acaaaagctg
    20761 ctagtatctt gggtctgatc atggtaggcg caatgacgat cagtatggtc aaattcaata
    20821 ccgttttgag cttttcaatg aagggcgaag aactaatgaa tgtccaagaa atcctcgata
    20881 gtatttttaa aggaattgta ccgttattgg taacattggg ctgtttcaaa ttactagata
    20941 aaaaagtcgg cgtcatcaaa att
//
LOCUS       DAZUMV010000038        20830 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000038 DAZUMV010000000
VERSION     DAZUMV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.038"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..972
                     /locus_tag="ACG8FK_001279"
     CDS             7..972
                     /locus_tag="ACG8FK_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5096895.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1029..1259)
                     /locus_tag="ACG8FK_001280"
     CDS             complement(1029..1259)
                     /locus_tag="ACG8FK_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS5096896.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1345..2232)
                     /locus_tag="ACG8FK_001281"
     CDS             complement(1345..2232)
                     /locus_tag="ACG8FK_001281"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5096897.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2321..3544)
                     /locus_tag="ACG8FK_001282"
     CDS             complement(2321..3544)
                     /locus_tag="ACG8FK_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5096898.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3561..4607)
                     /locus_tag="ACG8FK_001283"
     CDS             complement(3561..4607)
                     /locus_tag="ACG8FK_001283"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5096899.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4771..6498)
                     /gene="ptsP"
                     /locus_tag="ACG8FK_001284"
     CDS             complement(4771..6498)
                     /gene="ptsP"
                     /locus_tag="ACG8FK_001284"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS5096900.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(6498..6764)
                     /locus_tag="ACG8FK_001285"
     CDS             complement(6498..6764)
                     /locus_tag="ACG8FK_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS5096901.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7243..9477
                     /locus_tag="ACG8FK_001286"
     CDS             7243..9477
                     /locus_tag="ACG8FK_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5096902.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9625..9945
                     /locus_tag="ACG8FK_001287"
     CDS             9625..9945
                     /locus_tag="ACG8FK_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5096903.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10150..10374)
                     /locus_tag="ACG8FK_001288"
     CDS             complement(10150..10374)
                     /locus_tag="ACG8FK_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFS5096904.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10572..12152)
                     /locus_tag="ACG8FK_001289"
     CDS             complement(10572..12152)
                     /locus_tag="ACG8FK_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS5096905.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12553..13929)
                     /locus_tag="ACG8FK_001290"
     CDS             complement(12553..13929)
                     /locus_tag="ACG8FK_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5096906.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14057..15145)
                     /locus_tag="ACG8FK_001291"
     CDS             complement(14057..15145)
                     /locus_tag="ACG8FK_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5096907.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15317..15751
                     /locus_tag="ACG8FK_001292"
     CDS             15317..15751
                     /locus_tag="ACG8FK_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5096908.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15829..16362)
                     /locus_tag="ACG8FK_001293"
     CDS             complement(15829..16362)
                     /locus_tag="ACG8FK_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS5096909.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16456..17634)
                     /gene="mutY"
                     /locus_tag="ACG8FK_001294"
     CDS             complement(16456..17634)
                     /gene="mutY"
                     /locus_tag="ACG8FK_001294"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS5096910.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17627..18424)
                     /gene="recX"
                     /locus_tag="ACG8FK_001295"
     CDS             complement(17627..18424)
                     /gene="recX"
                     /locus_tag="ACG8FK_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS5096911.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18531..19913
                     /gene="rlmD"
                     /locus_tag="ACG8FK_001296"
     CDS             18531..19913
                     /gene="rlmD"
                     /locus_tag="ACG8FK_001296"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5096912.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20087..20665)
                     /locus_tag="ACG8FK_001297"
     CDS             complement(20087..20665)
                     /locus_tag="ACG8FK_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS5096913.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         6208 a         4232 c         3633 g         6757 t
ORIGIN      
        1 gatcctatgt taaaagaaaa aatgaaaact ctccccatgc tgccgggtgt ctatcttatg
       61 aaagaccgag atggtaaggt catctatgtt ggtaaagcaa aacggctcaa aaatagagta
      121 agcagctatt ttcatcagaa taaacagcat tcaaaaaaag tattacgtat gattcatcat
      181 ataacagatt ttgatttcgt cgtagtagac acggaattag atgctttatt gctcgagtgt
      241 caattgatcc aacattaccg tcctttttat aaccgacaaa tgaattattt cagtaattat
      301 aattatgtac atattaccaa taagggattt gtattaacag atactcctac ggctcgaaca
      361 tacgggcctt ttcgtctcta taaaaaaatg ccgtctattt tgcgtataat ggaagaaact
      421 tatcaaatgc cttggttgtc tgaaattagt ttgttagctt tgcgagtcca gcttcctgac
      481 ctacaggaaa tgacttttga acagaaaaaa aaggaactcc aagggctgtt tcaaggccga
      541 aataaaaagc tactgactta cttgaaaaaa agacaacaac atttcatcta tcagttgaat
      601 ttcgaaaaag ccggtatgct tcaaaaagat atcgaattag ttacttattt tattagacgg
      661 atccaggaac aaaagcagtt tttaagaaca ccatcgctca ccttcagcat gccattagca
      721 gcagatgaga gtcagaaaaa gcactacctc gtttgttatg gtcaacttgc agaaacaatg
      781 attacctctg gtgacgtttc tcctgacttc tattatgaaa agaaagaagc tcatttatct
      841 ttgaaacgtc aattgtcaaa agaagaaatc gatcctgtac aaatcttgat cagttaccgc
      901 aaaaaattag aaaaagaaca gtttgaaatt gaactgttaa acaaaaaaga agctgaaaag
      961 caactaaatt agttgcttct cggcttcttt taggcatctt caacttcaaa tgtcaattat
     1021 cctaaaattt attggattaa gtcatagatt tccatcgcta cgatatcgat attatcaaat
     1081 tcgtattgct gatgagtatc tgtttcagtt aattcaaatg tatcggtcgc tttatcataa
     1141 gttactgtac acttctctac gccttctttt tcaaaacggc gaacttgcag ttcattatct
     1201 gctgactcga ccatagcctc cagtcgtttt atgattgcga ctaattgtga atgtgtcatc
     1261 ggaggtcctc cttttcttat ttctgttata ttgtatcaaa agcatagcct acttgcattg
     1321 attttctcta aaaaaactcg ttttttatga ttttttttct ggtatttttc cttcaggcca
     1381 ccaaagtttg atcaaagctt gggttccgtc attctcaaaa cctttatctg ccaactcttc
     1441 atatagtttc aatgcttggg tagttgctgg caatacaaga tccatttttt ctgcttcttc
     1501 taatgcaatc tttaaatctt tgataaaatg tttgacaaaa aaaccaggtg tgtaatcttc
     1561 ttttaaaata cgaggagaat aattgcttaa agaccagtta gctgcacttc cacctgaaag
     1621 cgtctcaatc actttcttta aatcaagtcc gtttttttgt gcatagacaa gcatttccgt
     1681 cattcctgtc atcgtacctg caatcatgat ctgattagcc atttttgtat gctgaccttt
     1741 cccagcagaa ccatgcaatg taaatgtcgt accaaattct ttgaataaag gcagtacttg
     1801 atcataaacg gcttcttctc caccgaccat gatcgtcagt gtaccattct ttgctcctaa
     1861 gtcaccaccg gataccggtg catctaacgc atctgcgcct ttttcttttg ctgtttttgc
     1921 aattttttct gccaggcttg gtgtacttgt agtcaaatca actaaaatat gtcctgagat
     1981 atctgcagaa aaaatccccg actgtccata gtagatttct tctacatctt tggggtaacc
     2041 taccattgta aagagaatat tactttgttc tgctaccgct tttggtgtat ctgcccagat
     2101 agctccttct gttactaaag gatcagcttt tgcttttgtc cgattataga cagctacttc
     2161 gtgtcctgct tctagtagat ggcgtgcgac agcacttccc atgacgcctg ttccgataaa
     2221 accaagtttc attttttcga cctcctaaaa atcaaaaatc cttgcttcta tgctagtata
     2281 cgctagaaac aaggattttt tcataaaaaa tgtttgaatt ttatctttta aatgaattta
     2341 attttatttt gattcgttca atcgagtctt cttcttcttt tcttcgcatc tcttcttcat
     2401 gataaacaac catgtcttga taaaattcga tcatagcttc gccgaaatgt gtagatgaaa
     2461 tatcataaag tttagcttct aaaacttttg gatctttagg aacttcactg gaaatataat
     2521 caataagtgc tggtgcaaag tcttcgtcgg tttcaaaagt tttccctaat gaaggatgat
     2581 cgaacaatcg gttcaaataa gcatttcctt ccacgacaca aggtgttccg gctgccatcg
     2641 cttctgtata agtaagccct tgtgtttctg aagtagaagc actgacaaaa taatctgccg
     2701 ctttataata aagtgctaca tcattgttgt ccacttctcc tgtgaactgc acaaattctt
     2761 ctaactgcag ttctgagatc aattcctcta actcttctac ataaggtcct tttcctacga
     2821 taactaatct ggcgttagga taagcggaga tgacctgagg aaacccatta atcaaggctt
     2881 ggatattttt ctcataagaa atcctgctta aagaaagaat catcaaatgg ttttcctgaa
     2941 gacctagtga ctcccttaat ttgctgatat cctgctgagt gatatcagaa cgtttgaatt
     3001 tttcaatgtc gataccagta ggaatcacgc gaagcggtgc tattacacca tagctgcgta
     3061 atgtatcaat aacacgttca cttggacaaa ctacacctgt cgtatgattt gcaaataatc
     3121 tagacaagta ttttacatgg gattgcctga tcacttttcc tttaccgata taatgaaggt
     3181 agtcttcata catcgtatgg taagtatgga tcagaggtat tttcaacttc ttagctacca
     3241 tcttcccaag tatccctgct ccgaattctg tatgggtatg aataagatct agttctagtt
     3301 ctttggcaac caaataagca taccacatgc ctctaacaac aactcttcga tccttgaaag
     3361 aaataaatgg tacacttggc attcgaatca catctttttc caacttatcc gcattaggat
     3421 cggtcgttgt aaaaatatag acttcatgcc catgtctttc taattcttgt tttaatgttt
     3481 tgatcgatgt agcaactccg ctgacttgcg gaaagtaggt atcggtaaaa aagccgattt
     3541 tcatgttttt cgcctccaaa ctagtccttg acctgattta ctttttttaa tttgctagag
     3601 actttttcat tcagtacact ttcgtataca tttttaagtt cttcaccgat tttattgata
     3661 cttcgctctt ttgctgtttg ataaccggct tgtgtcgtac tcggtagttc ttgattggta
     3721 atctgttgga tcagtcggat aaattcttcg tttgtcttcc ccatatagca gttcttgcta
     3781 tcttctaacc agccattata cactggaata tctcgaacaa gtacgttttg tttgcttgct
     3841 aaagcttcta aaacaacgat cccttctgtc tcttcataag aagggaaaaa gaatagatct
     3901 gcacctgaat acgcaccttc tatgatctct ccacgaatgt aaccaggaaa caggacattt
     3961 tcaggatgat cttcttttac aattttccgg atgttccgag gaatcgagta catgggaaca
     4021 tgaccaaacc aaataaatgt gtattcgggc atcttcttag caatttctac gaaatcaatg
     4081 attcctttac gttcgaaaaa caaaccgaca caaatgatta ctttttgatc cggagacagt
     4141 ttgaaatatt ctctgaactt ttgttctttc attggatcag ggtgatattt ctctaagtcg
     4201 atcccattgg aaatagcttg aatcggcaca cggataccat agctttccaa caactgtttt
     4261 gaataaggtg ttggcgtgat cagatagtcc gctttttggt acaatcctac cagatatttt
     4321 tttacgatag gagaaagctg gttcgatccg ataaaggaat ttcggaaatc ttcctctgta
     4381 gaatgcgcat gatagacaac tttttttcct tctcttctgg ctttattgac catattatga
     4441 ctattgattc cgtatgtatt gatgtgcagg atgtcataat cttctttttc atctagtgta
     4501 taagagatac cgacttccgt cagagcacgt ttttgatgat caagcgcacg cccgattcca
     4561 gattttgcaa gcatcttttc actttcaaaa tataataata ccttcacctt acgtccccct
     4621 tcacttatat agtataacat agcctacaaa ctgtcactca aacttaagcc ctatttttgt
     4681 ctattcttac tgacaaaaac gttttcttaa aaaagaacaa aaaaactggc gtagctcttt
     4741 tacaagccac accagctttt gatacgttgt ttattttaca tattcatgaa caagttcaac
     4801 gacttcttcc atcgtatcgc attctttcaa tgcacgatca gcaagttcag ccattttcgc
     4861 tgtgtccaag cgtttcatta aactacgcgt tttaagaata gatgtcgcac tcattgagaa
     4921 ctcatctaga cccataccta ctagcaatgg aacagctgtt tgatctccag ccatttctcc
     4981 acacatacct gcccattttc cttcagcgtg tgctgcatca attacatttt tgatcaaacg
     5041 taaaattgat gggttatatg gttggtacaa gtaagaaacg cgttcgttca tacggtctgc
     5101 tgccattgtg tattgaatca aatcgttcgt acctacagag aagaaatcta cttcttttgc
     5161 aaacttgtct gctaagactg ctgctgctgg aatctcgatc atgatgccga cttggatcgt
     5221 atcagatact tccttgcctt cacttatcaa tttttgtttt tcttcttcaa agatcgcttt
     5281 tgctgcgcgg aattctttga gtgtagctac catagggaac atgatacgta aattaccatg
     5341 aacagatgca cgaagcaatg cacgcatttg tgtacggaac atttcgtcgc cttgttcaga
     5401 taaactgata cgcaatgcac gatatcctaa gaatggattc atttcatgag gtaattgcag
     5461 atatggtagt tctttatctc caccgatgtc cattgtacga acaactacag gttttccttc
     5521 cataccttct aatacagctt tgtaagcttc atattggtca tcttctgttg ggaagtctgg
     5581 agaatccata tacaggaatt cagtacggta cagaccaact gcttctccac cattattatg
     5641 gacaccaact agatcttttg gtgttccgat gtttgcagct aattcaaaat gcttgccgtc
     5701 agctgtgaca gtttctgcat tttttaattt ttcccattct gctttttgtg ctgcgtaatc
     5761 tgcacctgct ttttcaaatt cagctttttg ttcatctgta ggatcgatga ttacatcgcc
     5821 ttcgatcccg ttgacagcca aaatatcgcc ttctttgact ttagcagtaa tttcttttgt
     5881 cccaacgatt gctggaattt ctaaagaacg tgccatgata gcagaatgtg aagtacgtcc
     5941 accaatgtct gttacaaacg cttttacaaa attgcgatct aattgtgctg tatcacttgg
     6001 agtcaagtca tgtgcaacaa cgaccacttc ttcgttgatc attgatggat ttggtaaagt
     6061 aacgcctaat aagtgcgcta aaatacgttt agcaacgtca cgaatatctg cagcacgctc
     6121 ttgcatataa gcattatctt ccattgcttc gaacataccg atatacatat ccgttacttc
     6181 ttttagcgct gattcagcat ttacgctgtt gtctttaata ttttgcttga tttgacctac
     6241 catttctgga tctgaaagaa ccatcaaatg ggcatcaaat acttgcgctt ctgcttcacc
     6301 taagctttgc gctgcttttt cacgaatttg ctgcaattct tcagtagatt tagctaatgc
     6361 agcatccaaa cgagcttctt cagccgcagt atcttctact gttgccttac tgaatgttaa
     6421 atccggttga actagcaggt aagcttttgc aacggctaca ccgtcactag cggcaatccc
     6481 ttttagcatt tcaaccatta ttcagataag ccttctttct tcattgtttc aacgattgct
     6541 gccattgcat cagcttcgtc agcaccttct gcagtaatag taacatcaga accttgacct
     6601 acacctaaag acataacgcc catgattgat ttcaagttta ctgatttacc tttatactct
     6661 aagtttacat cagagttaaa tttgcttgct gtttgtacta atagtgtagc tggacgagcg
     6721 tggatcccag tttctgctac tacgtgaaat tctttctttt ccatattaat cggtctcctt
     6781 taagtgagtg ttgtttttat gtgagggttt tcttttttag taattcagag aatcctaaca
     6841 aatgccaccc tttcatctgt aaagattaac attttttttc gttagataca acagttttca
     6901 ttcaatttca gataaaagta ttcaaattat tccggattct ttttcatcct tttcttcaca
     6961 ttttataaca aacgtgttta tttccttttt cttgtcgctt tctcttctcc tgacgtttat
     7021 cattctttcc tttgaccctc tttgttttca tatgaaacga attacttgct tcccttttta
     7081 tttttagtat aataagagtc aaaggtcagg aatggtcaat agagatttca ccttttctac
     7141 ctcataaatc tcttaaaaaa ctaggggtta ttcttttacc tcatgaaaaa cgagatgtaa
     7201 aatatggatg tacccatttt tgtgaaagga cgtgaatgat tcatgctttg tcaaaactgc
     7261 ggcaagaatg aggcaacgat tcacttgtat gccactgtca atggtcagcg gacacaactc
     7321 gattactgtc agagctgtta tcaaaaaatc aaaaatcaac aaaatggagg tctgactaat
     7381 atggcccaaa atgatccatt tggatttggc agtttggacg atcttttccg ttccatgtct
     7441 cgccaaatgc aacagcaagg aaattatgag caaacaccac caacacaatt tggcggaaac
     7501 aacaacttca atggtggtca gccacctcaa ggcaatacag aaggtttatt gggagaatac
     7561 ggaatcaata tcacagaaca agcacgacaa ggtgatatcg atcctgtgat cggacgcgat
     7621 gacgaaatca aacgagtcat tgaaatttta aatcgccgaa ccaagaacaa tcctgtcttg
     7681 attggagaac caggtgttgg taaaacagca gttgttgaag gtttagcaca aaaaatcgta
     7741 gatggtgatg taccgcaaaa attaatggac aaagaagtca ttcgtttaga tgtcgtatct
     7801 ttagtccaag gtactggtat acgaggacaa tttgaagaac gtatgcaaaa attgattgaa
     7861 gagatccgtc aagcagaaaa tgtgatccta ttcatagatg aagttcacga aatcgtcggt
     7921 gctggttctg ctggtgatgg aaatatggat gctggtaata ttttaaaacc agctttagct
     7981 cgaggagaat tgcaaatggt tggtgctact accttaaatg agtaccgcat catcgaaaaa
     8041 gacgctgctt tagaacgtcg tatgcaacct gtacgggtag atgaaccaac tgtcgaagaa
     8101 acgatcagta ttttgaaagg tttgcaaaaa cgttatgaag actatcatca tgtaaaatat
     8161 actgatgagg ccatcgaagc agccgcaact ttatccaatc gttacgtcca agatcgcttc
     8221 ttgccggata aagccatcga tttgctagat gaaacaggtt ctaagaaaaa cttgacgatc
     8281 caaatcgttg atcctaaaac aatcgagaaa aaactgcaag aagcagaaga acaaaaagtt
     8341 ttagcttctc gagaagaaga tttcgaaaaa gcagcttatt atcgcgatca gatcaataaa
     8401 cttcaaaaaa tgaaagaacg tcagctcact gaagaggaaa cacctgtgat taccgaaaaa
     8461 gatatggaaa aaatcgttga acaacggaca ggtattccag tgggtgaatt aaaagaaaaa
     8521 gaacagacac aactgaaaaa cttagcagat gacttgaaag ctcatgtcat cggacaagat
     8581 aacgctgtag atcgagtagc gaaagcgatt cgccgaaacc gtgttggatt aaacaaacag
     8641 aatcgtccaa tcggctcctt cttatttgtt ggaccaactg gtgtaggtaa aacagagctt
     8701 gcgaaacaac ttgcctacga actattcggt tcacaagatt caatgattcg tttcgatatg
     8761 tctgaatata tggaaaaaca tagtgtctct aaattgattg gttctcctcc aggttatgtc
     8821 ggatacgaag aagctggtca attaacagaa aaagtacgga gaaatcctta cagtcttgtc
     8881 ttgttagatg aagtagaaaa agctcatcca gatgttttgc atatgttctt gcaaatctta
     8941 gatgatggtc gattgacaga tgcacaagga agaacggtca gctttaaaga tacgatcatc
     9001 attatgacaa gtaatgccgg aactggtaaa gtagaagcaa acgttggttt tggtgcggca
     9061 cgcgaaggtg ttactcgttc tgtattgaac caattaaaca actactttac accagaattt
     9121 ctgaaccgtt ttgacgggat catcgaattc agtgcattat caaaagaaaa cttgatgaca
     9181 atcgtcacac taatgctaga cgatgtcaac caaatgctgg cagcacagca acttcacatc
     9241 gaagttccta cgaatgtcaa agaaaaatta gttgacttag gctatgatcc atctatgggt
     9301 gctcgtcctc ttcgacgcac gatccaagaa caaatcgaag atggtatcgc tgaattttat
     9361 cttgatcacc cttctatcca tgagttgaaa gcaaaactgg ataaagatgg aaaaatcatc
     9421 gttacttcta aaccagaacg tttggctaaa gaatcagccg aagaaacggc tgaataaatc
     9481 cagttttata gcgtgagacg gtatttcttt gagaaatgcc gtctcattct tttttcagaa
     9541 aagtcaaaat actgttcatt ttataaaaaa gaaattataa taagagaaac aagcaattaa
     9601 aaatacataa aagaggtgag cgccatgaaa ttggtaaatg taacaaacag ccattcccgt
     9661 cttgtattaa accagcttga aaacactgat gcccatctag tcaaggtcta cacagccgga
     9721 aataccacta ttgtctacac tgaagcccct gaacataatg aaattctgct gatcaatgac
     9781 aaacgtaaaa tccagccgaa agaaatcgaa gatgctaaaa attatttctt agataaaatc
     9841 aaagacgccg tttaccacga ggatgaaatc aaagtaatcg aattggcagg ccttgttgaa
     9901 atctcgattc caaaacatga attaggcgct acattagccg tgtaaacaaa ttttgaacat
     9961 aataattaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt
    10021 ataaacggcg aaagcagaag caactccttc ggaaataagc tgaaattcac gaaaattgct
    10081 tttcaaattt ttgtgaattc cagcttattt cttggagtta aacgcttctg tctcagcctc
    10141 tttttctttc tagctttttg atttttctct tttcttttgt acttttctct tttcattttc
    10201 agttctacgc gtgtctttat ttttaacttt ttcctcttta tggatgattg atcgtacttt
    10261 ttgtgcaatt tgctcgtttt tgtcccagtc ttttacatct aataatcgac ataatcgtaa
    10321 catctcttct tttttctgtt tttttctttc acgtctttgc cattctgttt ccatgtgtca
    10381 cactctcctt tctttaatca tatgtacgca aaataatttt tatatttttt tgattagaca
    10441 tttctatgat tttaatatag taaaaagagc cgtcacggaa aatccgtcac ggctctaaac
    10501 aaaatatgag aggctgtttt tgtttggcta ttcttattac ttgatgcagg gcagcttgtg
    10561 tcaagacttc tttacatcaa actttttaat tttacattgg gatatttatc cgcaaaccag
    10621 cgttctgcaa attggttctc gaataagaac aacggttggt cgaaacggtc acgagccaag
    10681 atattgcgac ttgaactcat cttctcatcc aaatcttctg gatcgatcca gcgagcgatt
    10741 ttgtgtccca ttggagacat gattacttcg gcgttatact catttaacat acgatattgg
    10801 aatacttcga attgtagctg accaacagct ccaatgatat attcttccgt tagatatgtt
    10861 ttataaagct gaatcgctcc ttcttgaact aattgataga ttcctttgtg gaaggatttt
    10921 tgtttcatga cattttttgc agtcactttc ataaacagct ctggagtaaa tgaagggagt
    10981 tcttcataag caacttttag ttttccttcg aacaatgtat ccccaatctg ataattgcct
    11041 gtatcatata caccgatgat atcgcctgct actgcttttt cgacgttttc tctcgaatct
    11101 gccataaatt gtgtgacatt gcttaatttc agttttttat ttgtacgttc caaccagaca
    11161 tccattcctc gttcgaatgt tccagaacag attcggacaa aagcgattcg gtcacgatgg
    11221 gctggattca tatttgcttg gatcttgaat acgaaacctg aaaattcttc ctcataagga
    11281 cttaccatct gatcatcttg tgttttgtgt ccataaggag ctggtgccaa ttcaacgaaa
    11341 gtctctaaaa aagtctgtac accgaagtta gtcaatgcag aaccaaaaaa tactggtgtc
    11401 tgatcgccac gagcaatttt ttcttgatta aatgcatctc cagcctcttt gattagttct
    11461 acttcttcta agacttgttc atataaggaa ttttcagtta gaggatgatt ttcgggaagc
    11521 agtccatctt catccaattc tactaatcgt tcatcctgat aattttccgg acgataaaac
    11581 tctacacgat tatggtagat atcataaaga ccttctagcc ctttacccat accgattggc
    11641 caattcatcg gataagattc gatatctaat agctcttcta attcttctaa caattccaat
    11701 ggttcacgac catcacgatc tagtttgttg ataaaagtaa atatcgggat gccacgtttt
    11761 tttacaactt ggaataattt ctttgtctgt gcttcgatcc cttttgcact atcgattacc
    11821 atgaccgcac tatccacggc catcaaagta cgataagtat cttcagaaaa atcttcatgt
    11881 cctggtgtat ccaagatatt tactcgtttc ccttgataat caaactgcat aacagaacta
    11941 gtaacagaaa tcccacgttg tttctcgatt tccatccagt ctgatttggc aaaattacct
    12001 gtttttttgc cttttacggt cccggcttga cggatagccc caccaaacaa tagtaattgt
    12061 tctgtaatcg ttgttttccc agcatccgga tgggaaatga tcgcaaaagt tctgcgattg
    12121 tccacttgtt gctttaattt tggattattc ataacctaac tccatttctt ccattcaatt
    12181 atttatattt tctctaggca cagattgtcc gatctcttaa agaaagacaa tttcatatag
    12241 ggctgtgaca aaagccgtgt agcagtctct ttttccgagt aaacggcgtt ctagagacag
    12301 cctatcagca ataagcccac ccaagccaaa aatacgaaaa gctgtttttg cttggctgct
    12361 cttattactt aatgcaggat agctttttca caacctttgt caactactac aactgaatca
    12421 gtatagcatg gattcagcga atgaaaaagc caccaaacca taagagtcaa atattttttt
    12481 gtcagctgac taaaagaaaa gacgtgttgc ttgtttattc aagcaacacg tctttcataa
    12541 ccaagtttcc aattatcttc ttggctcgtc atcttcgaat tcttttctga agtatcttcg
    12601 ttcttcttca ggttccgctt ctgtttcttc cggatcatga aaaatgacac ggattttaag
    12661 gattcgagaa ccttccattt cttcagaaac cagcgtaata ttatcgacat caaacgataa
    12721 cttttcgcct tcatccggaa tcatgcctaa tgctgtaatc aaataaccag ccatcgtatc
    12781 tacatcactc atatgcagat cactgtcaaa agcttcattg aactcatcaa tcagcatacg
    12841 tccttggatg atatattcgt gttcatcgat tttttcataa agattctcaa cttcatctga
    12901 ttcatcatcg atttctccga cgatttcttc taaaaggtct tctagtgttg ctaacccaac
    12961 gacgccgcca tattcatcta ataaaatagc catctgattc tgcgtctttt tcatttcata
    13021 taacagatca tcaataaaga ccgtttcagg aacgaacaaa ggctcctgca tgattttctt
    13081 gatatctaaa ttatcaaagc cgaatttatg ggcagctttt agtaaattct ttgtatgaag
    13141 gattccgact actttatctt tgtcttcgtt ataaacaggg attcttgaat agttttcgga
    13201 taaaacttca ttcacatttt cttccacagt gtcgttgata tcaatcataa atgcatccgt
    13261 tcgtgggacc atgacttctc gggcaacttt cgtatcaaga gaaaaaaccc cttggagcat
    13321 ttctaattct tcattattca atacgccttc gttttcaagc atataacgca tttcatctct
    13381 ggtcattttt gaatcttcat catcaaaagt catcggcgtg atacgcgcca ataaacttgt
    13441 ggaagcagaa agcagccaga cgaagggctt agctactaca ccaatcaagc gtatcagccc
    13501 tgaagtgaac tttgcgactt cttctgattt atttaaagca atacgtttag ggtacaattc
    13561 tccaaaaaca atcgatacat aggtcaaaat agccagcaca atgatatttg caatattttt
    13621 tgccgctgtg ccgccaccta ataccggagc taaccgttcc gataaagtat tagctagtga
    13681 agcaccagac aaaatgttga ccaaagtgat tcctacttgg attgtcgata ggaaattatt
    13741 tggattctgc aatattttta aaagcttttg agatttcaca tctccttcat cagctttttg
    13801 ctcgattcga ttcttattga tcgaaacaac agcaatttcg gaagctgcta aaaaagcatt
    13861 gatcaatgtc aaaacgatca ataataaaat ctgcgctaac agcgactgac tctcagggtc
    13921 agcattcata atcgcattct ctccttataa aattaatttt tagtttacga tagaaaaaaa
    13981 cgtggcacta tagccacgtt aaaatggtat cacaaagcaa ttttctttgc aataaaaaca
    14041 agtgtactaa aactacctac gaggtcgtag caattttctt cttgttttca ttttgcttgt
    14101 cttctttgac gattttgatt atataggaaa tgatcgtctt gcagattgcg taaaaaggaa
    14161 cacctaagaa gataccaagc aatccggcaa tatttccagc tactagaaga acgataatga
    14221 tcgttaatgg atggattttc agtgatttcc caattacatt aggataaatg atattcccat
    14281 ccaactgttg gacgattagc acaaccacac aacaaagtaa tgcttttacc ggatcattga
    14341 agactgttac tagaaatgca ggtgctagtc ctagataagg tcctaaatat ggaattaggt
    14401 ttgtcaatcc cgcaatgacc ccaaataaga aagcataatc cacaccaatt accatatatc
    14461 ctaaaaatgt gaaggtacca acgaataaac attcgattgc ttgtccgcta atgtaattag
    14521 ccaatgtttt attcagttgc cccaataact ccacaatttg gtttcttctt ttttctggga
    14581 aaaactgttg gatgtttggc actagttttt ctccgtcttt caacatatag aacaagatga
    14641 atggtgctgt aatgattact acggttgccg aagcgatcgt tccaacgatc gaacctaaac
    14701 tattggatac tccactaagg aattgctgaa tgattgtccc gtaagaaatg tctaattgct
    14761 caaaatattt attgatatct acttccttga acaaaggcaa ttcggctacc tcttttaacc
    14821 agctttctac ctgtttgatg aaatcaggca tattagatgc caatgaggaa atttgctgta
    14881 ctagacttgg gatgacacta agtaaaatcc aaataattgc tgcaactaaa aggagcagca
    14941 caattgcgac agcccatatt cttttgattt ttgctttcat caacagcttg acaataggat
    15001 tcaataaata gtaaagaaag cctgcaatca gtacaggtgc aaataacgtg gaaaaaaatg
    15061 tcccgatcgg ttcgaaaata aagtttattt ctgatgaaat catgatcaat gcagctatga
    15121 tcaataattc aagcgaccaa aacattagtt tagactgtct aagtttttca aacaatagat
    15181 ttcctccttt ctttcttact agtatatatt atattcgtta aaaattcttt tattcttttt
    15241 ttatttttga aaacttgcca tactttttat ttgttttatt ttccagtata ctatacgaaa
    15301 ggaaggtggc tagaccatga aagttgtaca aacaaaagat acaatgagca atatttactt
    15361 agatgctgta cggatcaggc atcaagtttt tgtagtcgaa cagggtgtgc ctttgtctcg
    15421 agaaatagac aaagatgaag cccattgtat ccacttcgtc ctgtactcag ataaaaaaga
    15481 accgcaagga actgtacgtc ttttgccttt agaaaacgga aaaatgaaat tacagcggat
    15541 ggcgattctt tctgaataca gacaccaagg gttaggaaaa atattgattg aagaagcaga
    15601 aaactttgct aagaatcaag gctataatac gattttatta ggggcacagt ccacagctga
    15661 aactttttac gaaaaactag gatatacagc ttacggcgat ccttttgaag atgctggcat
    15721 gcctcatatt tatatgaaaa agactttata aaaatttatt tcgaaaaaag caaaaaacag
    15781 caagcttttg agaaaaatct ctcactggct tactgttctt ttttgtgttt acttacgcga
    15841 caattgcttg taacggttat accaaatatc aatatagcct tccgagaacg ggccatctcc
    15901 attattgatc cagtcaacca gaatcttcac attttctttt aggatgaagt caatatcgtt
    15961 cgggtaattc atcattttgc tgtgcatctc atattcatcg acatccaata gtcgtttctc
    16021 gccgtcagga aataccttga tatccaagtc atagtcgata tatttcaagg cctcatcgtc
    16081 cagcaaaaaa ggtgaagcta gattacaata ataggaaacc cccttttctc tgatcattgc
    16141 tattacattg aaccagtatt ttttatgaaa ataaacaatc gctggttcgc gcgttaccca
    16201 tcggcgtcca tcagattcag tgactaatgt gtgatcattc acgccgatca tagagtgttc
    16261 gcttgttttt aataccatgg tatctcgcca cgttcgatgc aatttgccgt cgtgcttgta
    16321 actttggatc gtcacaaact ctccttcttt tggaactccc atagcaaccc ttccttaagc
    16381 agcattttgt gctttgtgtt tgggtgttca ttacaccgac atcataatta ttatatcaat
    16441 aaaaatgagc tttgcctaga aaggattcct attgtttcga tttttctcta actgatctac
    16501 cagtttcatt tggaccttag gaaaaacgac cgaatcaaat gcatcaggct tcaaccactt
    16561 cgaagttttg ttctcggtaa attcttgttc tgctccctcg gttgcccgtc cgtaaaaaag
    16621 gagaacatgc catttcagat gactaaaaat atgagtaact tctcctaaat gtcgtgtctg
    16681 ccaaacaaag agttcctgct tttcaaatgg gagattttgc tcttcttctg ctactaggtc
    16741 atcaaacaga tcgatttctg gctttgtttc ccattctttt ttcaatcgct catactcctc
    16801 ttgtgtcact tccatcattg gaaatgtcca catattcgcc agcaattttt ggctatctct
    16861 ttcttcaaaa taatacgctc cagaatgatt ttgaagagct gcactgatat aataaacgtc
    16921 ttttggcttt gctttttttg tttttacagg aaaactcgtt tggattcctc gtttgttagc
    16981 taaacaaaag gcttggatcg gacaagtctc acactttgga gaagttggtg tacagatagc
    17041 tgaacctaga tccatcattg cttggttgaa ttcacctgga tgcttttcat caatgatttt
    17101 ccgcatcgct tcatcaaaga ttttcctgct agaagctttt gcgatgtctg cttcaatgca
    17161 aaacaatctg ctgactactc gcatcacgtt gccgtcaact gcaggttctg gaagtccaaa
    17221 tgcgatactt gcaatcgcgc ctgtcgtata tggtcctatt cccttcaatg agctaatttc
    17281 ttcaggcgtt tgcggcattt ccccatcaaa ttctgacatg atctgtttag cagcagcttg
    17341 gatgttccgg gctcttgagt aataaccaag gccctcccat gcttttaaaa gcttttcttc
    17401 tggagcattt gctagctctt caattgtagg aaaccattcc atgaaacgat aaaaataatc
    17461 gatcactgtg tctactctgg tttgctgaag catgatctca gagatccaga tacgataagg
    17521 atcacgatta tagcgccaag gcaggtttct tttttcttgt tcataccatt gaatgaactg
    17581 atcttgaaat tcttttgttt ctttatctgt ccatttttcc cattctttat tcactttcct
    17641 cttccttttc cgtaatgaat cgctggatca tatccaaatc aaatcctttt tgatataagc
    17701 tttgctttac ttttagattt ctttttttag gttcaaacct taaatttctt cgccatagtt
    17761 tatctccttg tttgactaaa taatcatatt ctatttcttg atctgcttct tgcggaagat
    17821 cagctatcgc ttgttggatc acttcttgag aaaagccttt cgtcatcaat gtttgttgga
    17881 tttttctcaa taattctttt tggctttttc cgtgattttt tccaaacgct ttttcggctg
    17941 tctgatgtgc attttgaact tggtcttcaa atgtatattg gctgagcgct tgttcaatga
    18001 tttcaggttt caaacctttt tgctgcatct gctgcgctaa tttctttggt cctttgtcac
    18061 ttaagcgcat attcgtgcgc acataacttt ccccatagac caaatcatcg acaaggtcca
    18121 attcttttaa gcgggctacg attttatttc gatcttcttg aaggatttcc ttttctttta
    18181 agtatgttcg gatttctttt tccgatcgca actgataact taagtaattc attgctaggt
    18241 gaaaaccgaa atcagctccg ctactcttct tgatctcttg gatagtttct tcgtctaatt
    18301 cccgtccttt taataatcga tagcgtacta atacatcttc agatacttgt aaagcttctc
    18361 cttcagataa atctacgcga tagaaagggc ctttgccttt actgattctc gtaacggtta
    18421 acataaacat cctcctcgaa catatattct ctatcatacc acagaaaatc cccgtaatca
    18481 ggtattatat gttagaataa accacagaaa aaagaaaaag ggagatactt atggcagaaa
    18541 caattgtcaa aactggacaa acggttcctt tgaaaatcaa gcgtttaggc ataaatggtg
    18601 aaggaatcgg ttatttcaaa cggttgatca tatttgttcc atatgcacta ccaaaagaag
    18661 aagtactagt gaaaatcacg aaagcaactc ctcgctatgc agaaggagaa ttaataaaag
    18721 tcaaaaaaac aagcaaagat cgcgtagtgg caccatgtcc tgtttactat gaatgcggtg
    18781 gctgtcaatt acaacactta gcttatcaag ctcaattgga ttttaaaaag gatctgcttt
    18841 tgcaagcatt ggaaaaattc aaaccagcag gctttcgatc ttatcaactt ttgccgacga
    18901 tcgggatgga tgagccttgg cattatagaa acaaagcaca atttcagtta cgaaaaaaca
    18961 agcagactca aaaaattgaa gctggtttat acgaagcaaa ctctcatcag ttagtcccta
    19021 taacagactg cttggttcaa gaacctcgaa cacaaaaagt aatgaatact gttgttcaac
    19081 tattgaataa atttgatgca cccatctatg atgaacggac aaacagtgga atcttccgca
    19141 caatcatggt tagaatcggt gttaaaactg gggagttaca agttgttttt attactcgca
    19201 gtaaaaaatt cccgcaaaaa aacgcaatga tccgtgagat caatgaacga ctgcccgaag
    19261 ttgtttcaat catgcaaaat gtccaggcac aaaaaacgtc tctcgtgatg ggggatgaaa
    19321 cgatccactt atggggaaaa gaagccattg aagaacaaat caatgaagtg actttcgatc
    19381 tttctcctcg tgccttcttt caactcaatc ctcagcagac cgaattgctt tatggcgaag
    19441 gaatcaaagc attagatgtc cagcctaatg aaacgatcgt ggatgcttat tgcggagttg
    19501 ggacgatcgg cttaagtgta gcaaaacaag ccaaagaagt acggggaatg gataccgttc
    19561 ctcaagcaat cgctgatgca aaaaaaaatg cggaaagatt aggatatcac aacactcgat
    19621 atgaagtcgg gacagcagag caagttctgc ctaaatggtt gaatgaaggt tttcagcctg
    19681 atggtatcat cgtagatcca ccacgtacgg gtttggataa cgcattgatc caatcattat
    19741 tgaaataccc tccaaaaaaa cttgtttata tctcctgtaa tgtttctaca cttgccagag
    19801 atctggttaa tttaagtaaa aagttcaaga ttgaatattt acagtccgtc gacatgtttc
    19861 ctcagactgc acgttgtgaa gttgtcgtga agatgacgag actcgctgac taaatgaaag
    19921 aggttgtgaa aaagtctgcc ctgtatcaag taataagaac agccaaacaa aaatagctct
    19981 tcatgtttta tgaatactgt acttgtgacc gaagagttaa caggaggaca ctatttattt
    20041 aaaaaaaggg ttgtgacata gtttttgtca cagccctttt tgattattat tggatattca
    20101 cgtctatcac ttgataaaat gcatttgtcg tatcgctgat attccatact gctaaaatca
    20161 cattatatcc ttttcgatcc tgcggaagtg tgatcgtttg gttgactgtt tttggtggaa
    20221 cagttgcttt gtcgtctatt tttgtgatca attcaaaatc actaaatttc aaaggttgat
    20281 taggattcca agttggtttg gtaatgtagt aatcccaagt acttgtccga tgttgtgctg
    20341 ttaaattcca agtaatattt aatgttcctg gattaaccat gtttttatgc catctgttag
    20401 atgtctgttc atctaaaggc tcaaagccgc tgactcctgc actagcaatt tttccatcaa
    20461 tgaatgtatt tttggtagct tcgatacttt gcggttccca ttgtgctctt ccgacatttt
    20521 gatttaagtt tcctgctgct gatgttccta ggtatgcacg gcttcctgga gaagtaatat
    20581 aaccatgcgc aaaacctgga atgctaaaac cgaacaaccc taaactaact aacccaataa
    20641 acaataacgt actaatcttt ttcatgtaga aacctccttc atttgataac cacattgtaa
    20701 acgctttatt gtattaacac aaacattatg cttttataat tttatgttga agtaaaaaag
    20761 gtgaaaaaag taagatgcag attataacag aagtagacga gaaaagtaca aatagaaaac
    20821 atgagaatca
//
LOCUS       DAZUMV010000039        19450 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000039 DAZUMV010000000
VERSION     DAZUMV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.039"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3658
                     /locus_tag="ACG8FK_001298"
     CDS             131..3658
                     /locus_tag="ACG8FK_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS5096914.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="ACG8FK_001299"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="ACG8FK_001299"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS5096915.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="ACG8FK_001300"
     CDS             7446..7787
                     /locus_tag="ACG8FK_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5096916.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="ACG8FK_001301"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="ACG8FK_001301"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS5096917.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /gene="pheT"
                     /locus_tag="ACG8FK_001302"
     CDS             9144..11564
                     /gene="pheT"
                     /locus_tag="ACG8FK_001302"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS5096918.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG8FK_001303"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG8FK_001303"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS5096919.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="ACG8FK_001304"
     CDS             12998..13735
                     /locus_tag="ACG8FK_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5096920.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="ACG8FK_001305"
     CDS             13749..14564
                     /locus_tag="ACG8FK_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5096921.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="ACG8FK_001306"
     CDS             14577..15224
                     /locus_tag="ACG8FK_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5096922.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="ACG8FK_001307"
     CDS             15240..15896
                     /locus_tag="ACG8FK_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5096923.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="ACG8FK_001308"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="ACG8FK_001308"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS5096924.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="ACG8FK_001309"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="ACG8FK_001309"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS5096925.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="ACG8FK_001310"
     CDS             18238..18756
                     /locus_tag="ACG8FK_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5096926.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /gene="cbpB"
                     /locus_tag="ACG8FK_001311"
     CDS             18863..19363
                     /gene="cbpB"
                     /locus_tag="ACG8FK_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS5096927.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6537 a         3340 c         4177 g         5396 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgtg cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca
//
LOCUS       DAZUMV010000040        19437 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000040 DAZUMV010000000
VERSION     DAZUMV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.040"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..774)
                     /locus_tag="ACG8FK_001312"
     CDS             complement(<1..774)
                     /locus_tag="ACG8FK_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096928.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQ"
     gene            complement(880..1680)
                     /locus_tag="ACG8FK_001313"
     CDS             complement(880..1680)
                     /locus_tag="ACG8FK_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096929.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            complement(2019..3128)
                     /locus_tag="ACG8FK_001314"
     CDS             complement(2019..3128)
                     /locus_tag="ACG8FK_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5096930.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            complement(3118..3975)
                     /locus_tag="ACG8FK_001315"
     CDS             complement(3118..3975)
                     /locus_tag="ACG8FK_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5096931.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            complement(4050..5885)
                     /locus_tag="ACG8FK_001316"
     CDS             complement(4050..5885)
                     /locus_tag="ACG8FK_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5096932.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            complement(5934..8324)
                     /locus_tag="ACG8FK_001317"
     CDS             complement(5934..8324)
                     /locus_tag="ACG8FK_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5096933.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            complement(8594..9841)
                     /locus_tag="ACG8FK_001318"
     CDS             complement(8594..9841)
                     /locus_tag="ACG8FK_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS5096934.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            10233..10649
                     /locus_tag="ACG8FK_001319"
     CDS             10233..10649
                     /locus_tag="ACG8FK_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096935.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            complement(10666..11403)
                     /locus_tag="ACG8FK_001320"
     CDS             complement(10666..11403)
                     /locus_tag="ACG8FK_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096936.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(11826..12389)
                     /locus_tag="ACG8FK_001321"
     CDS             complement(11826..12389)
                     /locus_tag="ACG8FK_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096937.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(12376..12903)
                     /locus_tag="ACG8FK_001322"
     CDS             complement(12376..12903)
                     /locus_tag="ACG8FK_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096938.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(12926..14299)
                     /locus_tag="ACG8FK_001323"
     CDS             complement(12926..14299)
                     /locus_tag="ACG8FK_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS5096939.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            14383..14862
                     /locus_tag="ACG8FK_001324"
     CDS             14383..14862
                     /locus_tag="ACG8FK_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096940.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(15066..15209)
                     /locus_tag="ACG8FK_001325"
     CDS             complement(15066..15209)
                     /locus_tag="ACG8FK_001325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096941.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(15212..15529)
                     /locus_tag="ACG8FK_001326"
     CDS             complement(15212..15529)
                     /locus_tag="ACG8FK_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096942.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(15640..15822)
                     /locus_tag="ACG8FK_001327"
     CDS             complement(15640..15822)
                     /locus_tag="ACG8FK_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096943.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            complement(15859..16659)
                     /locus_tag="ACG8FK_001328"
     CDS             complement(15859..16659)
                     /locus_tag="ACG8FK_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096944.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(16796..17080)
                     /locus_tag="ACG8FK_001329"
     CDS             complement(16796..17080)
                     /locus_tag="ACG8FK_001329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096945.1"
                     /translation="MTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMSRFLKEREK
                     KELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ"
     gene            complement(17211..17519)
                     /locus_tag="ACG8FK_001330"
     CDS             complement(17211..17519)
                     /locus_tag="ACG8FK_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096946.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            complement(17681..18211)
                     /locus_tag="ACG8FK_001331"
     CDS             complement(17681..18211)
                     /locus_tag="ACG8FK_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096947.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            complement(18295..18591)
                     /locus_tag="ACG8FK_001332"
     CDS             complement(18295..18591)
                     /locus_tag="ACG8FK_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096948.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRDIMVQS"
     gene            complement(18597..19340)
                     /locus_tag="ACG8FK_001333"
     CDS             complement(18597..19340)
                     /locus_tag="ACG8FK_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096949.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
BASE COUNT         5523 a         3932 c         2696 g         7286 t
ORIGIN      
        1 ttgatcaatt aataatcgta cactccaatt aaccgagttt tgatcactca aatatttata
       61 atctttgtca tcaaaaacca gttgccaaac tctggtacga acagtttctt cgtccacttc
      121 ctcatctgaa gagtattgcg ataatgcttc ttcaactata tactttacaa cttcctcaaa
      181 atcagtagga actttatcaa taaccacttc atagtcttct tgttttacga tattgggatc
      241 aatatgttca tccaaacata cctcagtgag tttttctttc acctctgtat ctctttctgt
      301 ttttaggtac tctgcatagt cttccttaga ccattgtttt tcaagatcat ccgctatact
      361 tttaataaag gattctttat ttaaccaatt atctgcggta tctatacctt caaactcaaa
      421 agctgttcct tctatacgtg atttgacgaa agcatcaatt ttcttgtatt gattattcat
      481 ttcccatgaa acacactcat cttcttgcgt aatatcataa attttttcgg aaaaactaac
      541 taattcattc cacgatttct cctcggcaaa ttgacttagt ttcgatgcga taaaaggaaa
      601 ttttgctttt aagtcatcca tattcttgat ctcaaattcg atattttctt ctcctacagc
      661 tgaaatccat gctgaaagat tgattgcaat atcgctaaag tcttctacat tttcgacatt
      721 tcctgtatta tcttttatta ctgcttctaa agccatatcg gttgttgcac tcatggtttg
      781 atctcctctc ataatagagc aaaaagccgg tttaaccggc tttttgcatg gtacgttttg
      841 tttggtagtt ttctgcttgt gtttatgttg aaataatccc tatagctctt cttttaatag
      901 ttcttcaact ttatctctta actctccctg accactctct aatagattta aatagctcat
      961 cagaatatcc gtttcatacg tttttaattc agctctatac tcactttctg aatatgatcg
     1021 taaataccca tcattgctaa atgcaactag atcatcttga aagttccatt ctcctaaatt
     1081 tgttttactc actgcttccc atggactatt actaaacatt tcattaaaga aatcttcgtc
     1141 gttttgatat accttcaatt caggaaaata gttgctgtct tcattgattt cttgcaccat
     1201 ttttccaagt tcttcttcag ataatttacc ttccaataat tttacttttt ctctgtcatc
     1261 tgcataagcc atttgaacct ctcctttttc tttttcaatt tcttcaggtg tttgttttgg
     1321 cgtaactttc aaatctattc cattgcctat tagacccatt agataaccat agaacgtttt
     1381 aaagtcactt gtagcaagag atttggcatc agggtatgta tcccctccaa ttttatctaa
     1441 aacaaaatca gcaatcgtta cccaaggaga atatatcgta cacactacat cccatgtagc
     1501 atcttcaata ttcgtccatg tcggaaagtc attttcattc aaatagtctt ttttcagtgt
     1561 ttcgatcaga gtttttccac tttcgttttg gcttagatat tcaacaaatt ctgcaccatc
     1621 aaacctgcta ctaaatagtt tagctaatga gtctaaagct gtttttgata tttccaccat
     1681 tactacacct cctattaata ttttacgatg acttgttttt cattcacaaa taagattttc
     1741 tacatcttat ctattgcttt atagcttttc catacattat ttctaaatac aaccaataac
     1801 cctattaaat taagcttttg agtttatctt ttctaataat attctgataa atattctgat
     1861 aaaaaaattt gttgtatttt tttcttgact ttttctttgg aattaagatc agtttttatt
     1921 ttatttaaaa gtgaatctag ctctattcaa aataaaaaag agatcaataa tagtatcaat
     1981 ccctttttac tgtactctat caatttttat aatcttcatt atgcttttct aaactctttg
     2041 attaaaagaa taagaacctt tagacttgct aatgctccta atacagtcca aatgactaaa
     2101 agcattttct gatgtttatt ggtaattcct gtaaatattg aacttgcctt ttcagcaaac
     2161 gttctgtctt gcatctctac gctaccttgt tccggaatat taagatcatt atttccatct
     2221 aattccttgt tggcacgatc aacatcctca aataaatcat tagaagatgt aacagtttct
     2281 ggcttatctg aaacggattc actatcaatg gcatcagcgt atgcggtatt gccaactgtt
     2341 acttgtggcg tgatgtattt tgctttttca tctcttagac ttctaactaa taaacgatcg
     2401 tagttctgag gatatggatg acaagtataa agtgttaaca tatctaaatc aggaatatca
     2461 gtcagtgttg aattctgacc gtaatttcta ataacgtctg ttcggaaaac cgtataaact
     2521 gctttttcac caagataatc tatataaatg tggtctccgg ctactaattg atcaacataa
     2581 agaaattgtg gtgacgtata accgccacga tgacctgcta ttactgtttg atgaccttta
     2641 cctgataaag gtagactagt agcactcatt tgtgccacac ctctatataa atgctcctgt
     2701 gaagcgccta gaaacaaaag ctctagcaca tttatttttg gaatatagac ataaccaacc
     2761 gctcctgaat aggtagatgg ttcaaaatct ttcgtttgat tttttgaaaa aacatctcct
     2821 gtgtatggat tgacctttcc ttcagcttca cgaatatcaa atgatccacc gtctgtttcc
     2881 tgagtattgt ctactccttt atctgttttc tgagtattgt ctaatccttg gttaccgctg
     2941 ttcgatttta tttgttcgac tttttttgcc acttcctctt ttgaaggagt ttgttttttc
     3001 tcaattttct cttggatttc cgccacattc tcttttgttt tgaattgatg atagagctga
     3061 tttgcaggat aaattgttaa aaccaaagtg caaataagag tcaaaaaaag tagtatttta
     3121 ttcttcattt ttcattcctc gtctctttgc tactattttt ttaataatga ctatcacagc
     3181 tattattaat aaaaccccta caactacgat aggataccag ttcatctgcg cctttacctt
     3241 taaatcttct ttcttctttt ctttctcaac aatacttttt tggtaaggta tgctaacccc
     3301 tctaacgagt aacctatgag aattatctcc aacaggtgta catgtcataa gtgttacata
     3361 atcttttcct gccacaggaa gcaaatcctc aaattcggtt ggtagaattg tttttatccg
     3421 atctacttga taagcaagtt ttttccctga aatatcaata aaaaatttat caccaagttt
     3481 gagcaactct aaatctgtaa aatatttttt attaggaatg cctgaatgag ctgatataac
     3541 agaatggttt ccaatcccac cagttggata atcagttccg gttaatattc ctgctcctgc
     3601 tgatagttgg aaatcgttta caccatcata aattggcaaa tccacattaa tctttggtat
     3661 aagaatttca cccactaatt cacctaattt atccttctga agatttccat ttgacggaat
     3721 attgttatta tcttcaggaa tcgtgactga ggtttgttct gtgtcttttc gtttcctatt
     3781 aacttctgaa tatctcttga agacttcttc attctccttt tcagaagctt catcatattt
     3841 ttccttatag gagtttagtg tttgttgagt ttggtagtag gtataagcct gagtaattac
     3901 aggatataga cctactccta aacatagcaa catcccaatt tttaagatga tcaacttgat
     3961 attcttcttt ttcatgctca tacctacttt tcacacactt tttaaaaaga aaccctagag
     4021 ccaaaaacac tctagggaat tttatttcat taagctttgt gttgttctcg tttgtagaca
     4081 acatatagcc ctgttcctgc cacacctgca accactagaa atactactgt tccaattcca
     4141 cctgtacgaa ctgtttcagg tgttttaagg ttcttgatgt ctgttacttc gctagaagca
     4201 tcatcattcg ataccacaaa tggaacatcg tttctagttg gtgtatatcc tgaaggagca
     4261 acaacttctt caagagcata atcattgatt tttttatctg tgattgttgg attaccttgt
     4321 ttatcaaaat ctacatcata ggtatattgc aaaccttcaa tttggaactt accgtctgat
     4381 ccggaaacga attcagtagc tttagattta tctgcaatcc aagtaacagc tttcgttgtt
     4441 ggatcttgtt ttaggtaagt aactttattc ggatcaattt tgttcttcac tacaaatttt
     4501 gctccactca atgctttgct tgtatctccg tctgttttaa tgaattggtg atcaccaatg
     4561 attgtataag cgtgatcaac tggtgtttcg tcttcgggtt catatccatt gttgtagatc
     4621 gttgaaacat cattcttaag tggtgtatcc attttagctt tactattagt atgagcgttc
     4681 atcaaaattg taaagaagcg atcccctgca tcaaattgtt tctgaagctt atccatacct
     4741 gattcagtaa agacatagtt tgtcgtttgt ccgtcacgtt cgatactgta atcagtatct
     4801 aaagtcattc cttcgatcac ttctgatgaa tcataagtta actctgcacc tagaacatct
     4861 ttgacaacat atttttcgta attaccaata ttaccaggaa ttgatggttt caatgtatat
     4921 tgaacatctt cgccagtatc aacggtttct ttatcaatta ccttagtaat tacaggtcgt
     4981 ttgtagttgg taacatcaag aactacttta gcagtctccc cacgtttaac gctaaatttg
     5041 aattttttac catctagtaa ataggttgtt ctattagcat ctgttcccaa atcggtttct
     5101 aggaaataat agtcacctaa ttccaaacca tccacagtta atttacctgt gctgtctgtt
     5161 ttataagttc caacaacctg atcagatcca tcatcttttc gcaattcaaa aacagttccg
     5221 gcaagagcct ttccatcttc gtctttttta tcaagctctg ctgagtttaa ttggaaatag
     5281 tttttaggat agacatgtac atcataatta aagtattgat catctgatcc gtttagttca
     5341 ggtacgttaa caatcatagg atacgcttct tcaactacac ctttgactgt tcctgtttca
     5401 acaatcaaat acaagttatc tgttaaatcg tctgtattta cttgtatagg tatgtctata
     5461 ttcccttgtg aatcagtctt cattctactt acttcactgt attttttgtt tagtaataat
     5521 cgtgctttag catcatcaat atgttgacct tttgtagtat cactatccgt ccatcccaag
     5581 gatttagctt catctttagt aagtttcaca taagagaact caacgccttc aagaggtttt
     5641 acaccatttg gtaaagctat ttctttccct ttgttatcaa tatctgagac agggttccca
     5701 tccccattag tatttgttac tttatggata tgaatcgttc cagttccttg ataattatag
     5761 ttttgttgtg catatgctac ttgcccccct agtccaaggc tagaagagat tgagcttgcg
     5821 cccaatccca ctcctagcaa agtaccaaat agcattaaat ttgaaaaaat tttttttgat
     5881 ttcatacgtc agccttcttt tttcatattt acttcttttc cattaacact tttttatttt
     5941 gctttgtgct gttctcgata atacagtagc accatactca atcctgcaat tagaaataca
     6001 agaaggaagg ctgatgtacc gattccgcct gttcttactg tttcaggtac aaggtgatta
     6061 attactgtat attcaaaggt ttgtccatcg tcagcaacgg tgatgccttc tttagaccat
     6121 ttccctgtat aaccattagg gttttgtgtt tcttcgattt tgtacgtttt gttggcaaca
     6181 atattaaatg ttgccaagcc atttctgtcc gttttttgta caacttttac acctttgtcg
     6241 tccgtcaaag tgaattctgc gtttgctaat gctttccctt gatcatcgac tttatgaact
     6301 ttgatcacac cttttttcaa agtgttttta gctgtatatt caaagacttg tccatcatct
     6361 ttgacggtaa ttccttcttg tttgaatgcg cctgtgtaac ctgttggtac ttttgtttct
     6421 tctaagttgt aagttttgtt cgcaataatg ttgaacgtag ccagaccagt tgcatccgtc
     6481 tttttaactt cttttacacc tttttcatcc gttaaggtga attctgcatc tgctaaaggt
     6541 tttccatctt gatcgacttt atgaactttt actacacctt ttttcaaatt atttgtagct
     6601 gtatattcaa aagtttgccc atcgtcttta acactgatac cctcttgctt aaatgcacct
     6661 gtgtaaccta ctggtactcc ggtttcttct agactatacg tcttgtttgc tacaatattg
     6721 aagcttgcaa gtccattttc atctgttttt tctacttgtg ttgcaccttt ttcatcggtt
     6781 aaagtgaact ctgcatttgc caaaggtttt ccatcttgat cgactttatg aacttttact
     6841 acaccttttt tcaaagtgtt tgtagctgta tattcaaaag tttgtccatc atctttaacg
     6901 gtaattcctt cttgcttgaa tgcgcctgta taaccagttg gtactccggt ctcttcaagg
     6961 ctgtaggtct tgtttgctac aatatcgaag cttgcaagtc cgttttcatc tgttttttct
     7021 gcttgtgttt gggatgaatc atccgtcaag gtaaattcag cattagctaa tggttttcca
     7081 tcttggtcaa cctttttaac ttttacttta ccttttttaa ttgtgttcgt agcagtgtaa
     7141 ctaaattttt gtccatcggt attaacagta ataccatcct ttttaaatga accttgataa
     7201 ccttcaggaa cttttgtttc ttctaagctg tatgttaagc cttgttgcaa atcaaacgta
     7261 gctaacccat catctccggt tgtcttaaca gatttagcat tgttattgtc agttagagtg
     7321 aactccgctc cttttaaagc atttccatct tgatcaactt tcttaactgt tacctgacca
     7381 acagctcttg tccatttagc actcaaagat ttagagtcct ttgtttctga agtagtgatc
     7441 ataactacgg catcttgata cacgcctcgt ccatcaaaag taaaaggatt ggttgtgcta
     7501 acattagcag tacctttaaa tgacaaagta ccacttggtg tagtcacagg tactttaata
     7561 gtaaaacttg aatttaattt cacagtgttg ttagtaattt ctttaccatc tgcatcataa
     7621 atcttcatac cagctggttt tgctgatgta aaagtgagct tcgcattacc agtcttattt
     7681 gatgctgttt tatatgtgaa agtgtgatat tttccttcgt cagctgtctt agaagctgtg
     7741 atagaaaact cagtgttggg cgtatctttt ccattcttag catagtcata aattttttca
     7801 aatgcttttt ttacacgagc tgtttctgcg gcggtatggt ttggatgtga ccatacaatc
     7861 tgtgaaactt taaagtttcc ggctaaaacc catgaaacta attgtgttgc ataccatgct
     7921 tctgtatcat ttcctgcacc cagttccttt gctgtttttg atggaaaacc acctttaaac
     7981 atacgataga actcatcact gccagctccc ttgtacttca agcttccggc tccacttcct
     8041 gttggggatg gcaattctga atccatacaa tatccaatat ttccatgaga atctttaaat
     8101 tgacttgaaa tcatccattc atttggatga ttcggcatat taaccctaat ataaccgtct
     8161 gtttttacgc cagtttctgt aaattttgca cttgtcggtt ttgtgacatc tgcttcagct
     8221 cttattgctg aaaaaggcat tataagcgta gataacacca ttactaaagt cgcaaaacaa
     8281 taaattattt ttgattttaa aattatcgtt ttttcatgct tcatctctcg ttactccttc
     8341 tttataccag ttaggcgaag aaaatgagcc acgatatgtc ggtgtggtaa tgaccttcgc
     8401 gcccattcct aagttaaaat caccacacct attaatcact tttttcgttg aaagcaagct
     8461 gtttcaacgc tctagtattc atagcatcac cccttttagt tctacccacc atgagcaaca
     8521 agttttctaa gtagttaagc aaggacaaat aaagactata cttaactact tagtatataa
     8581 acaatctctt ttattaacgt accgaaaccg ctaattgaga tacgttatat gctgtttcaa
     8641 atgatcctgc attaatccaa cttgccccaa acacaggatc gacaacatgg taaaaaccgt
     8701 ctttaaatcc atcaacggct actacatgtc gattccaaat agcatcgccc caaaacattg
     8761 atttatgttg agcaggttca aaggcataag ttacccacgc tactacagga tttccattta
     8821 caatctcatc ttttaatcct tgtaccgatg aaccagaaat atctacaaca tttccgccat
     8881 tagcaacacc ccatgaaact acaggagccg gattcatacc ttgataagtt ccatccacat
     8941 tgtgtctcca ttcaccagaa aaaccattat ttggattatt atcaggagat agtggcattg
     9001 atttaacaaa agtcattaaa tcttgatttg ttgcaacacc tttataatgt aacgcttcta
     9061 gcaaagatgt tccttcgcaa agcattgtgt taccttgact aataaatgga acgcctagca
     9121 caacattagt tggttgttcg ggtttacttg gtgaagtaga actgtcagtt tcatcactcg
     9181 aacctgtttg tgattgatca ctagaagatg tactggttga ttcatttgca tcatttgatg
     9241 ttgattgtga gttatttgtt gatgttccag tatcagtttc cgtagtgcta ctttcacttg
     9301 ttccctgtga tgcttctttc tcacttgtag aattatttgt attagatgaa cttgctccat
     9361 caacacttga agcacttgtt tctgtcttat cagaagtgtt tgctgttggt tccgattctt
     9421 ttgcagtctc ggattttttg ttctcttgct ccgtattttc aacaggatca ggtttatctt
     9481 tttgaacagt ttgactaact acttcgccat ctgcttcctt aactgttaat gtatctttac
     9541 tgatgttgaa agttcgtcca attgggatag tccgttgttc tccttttgcc aaatcaacat
     9601 tgttcaattc ggcaaactca gcaacagtag tcttgtaacc taagctttta agcttctcta
     9661 cagttgacca aatagtatct ccttttttga acgcataact tgattgtcct gttgtaattt
     9721 ggatttgatc cggtgtgttt gcagtccatt catcagcatg tgaggttact ggtactgata
     9781 accccgcaag tacagctgat gcaaataaaa ccataccaag cgaatttttc ttcatattca
     9841 ttccttcttt ttgtttttat tttctgtttg tgatcgcaat gcttctattc cttcttccgt
     9901 tataatatag gttttgtcac ccactttctt gaacgaacct ttaggcaaac gatttggata
     9961 atagttgatt atgtttgtaa cataatttct acctttaccc aaaatcaaac ttgcttccct
    10021 tgcggtgtac aagttgtatt tgtctaggtt caatttcttt ttgtttgcga tttagaccta
    10081 cccctttcgc aaaatttatt gtataataat agtaccattg cactgtcata attgcaatat
    10141 ttttttgggg tttatcttca tttaatagcc taaaaaggct tgttagataa ggaatatcta
    10201 actaatcaaa acaaaaagga agttaagaaa aaatgaacaa aaaaaggaaa ttatcagtat
    10261 taattacact tgtttctata ttgactattc ttagcggtat cttgtcgctg aaaatattca
    10321 atcaaataaa cacaagctca aatgccaaag atggaatagt cgtagaaaat acctctaatt
    10381 ctaaagaaac tgttactact acaaaaaaag acaaatctgc tgagatcact acgaaaaatt
    10441 atttaataaa gactgtttat tttgatgaac gaaaaacagc aattctcaaa gatgaggaag
    10501 gatcagaatt aaagctacct ataacggtat ccgataagtt caacggaacg ttctccttac
    10561 aaaccgctga aggatcacct gacaatctca aagttgggca aacaatcatt cttggtaaaa
    10621 acgacgaact gtattcattt gtaaaataaa aaaatggagc aaaggctagt caagcccttg
    10681 ctccattttc cattcttcaa gaggtgtagc tatttcaaca ccaaagttta aatactgtgg
    10741 atgctgtaga tacatgatat ccattctatt ttcgataaac tcttttaacg ttgagtagat
    10801 gtatttttct aatgctatat ctgcccaact accataatca agtttattcg tgatcttgac
    10861 gtttatttca atttgagaaa catcaatatt ttgaacatca ggaaacttat tcagtcttag
    10921 ctcttctcta accgcttcta ctagcggttc atattgtccg cctagttcgc taattgttga
    10981 aactatttca tctgaattcc aacgatcttc caataccttt gctacatttt ctttaggcgg
    11041 ttgtctttct ataaattctt caggtgtttc atcaaactct actctaaagt tcaatttatc
    11101 ttgatggtta gcatacatat aatcatctct gtttgattca aaaaagttac gaattcctgt
    11161 gtctgattta tcacttaact ccttatttgc taaagtagcc caaaaatccc aagttccttt
    11221 attttcagta tacaaagtaa aattaatgtt tatatagttt gcagtttcag gatacccttg
    11281 ctctctaaga gcatctttaa caatcactgc taagtcagtt tcaaaattct ccataaaaaa
    11341 ttcactaaat tcttttggat tccacgtttc ttctaataac ttttctaatt tacgatctgc
    11401 cattattatt ctcccccact ttactttcac tcttatatct aaaaactgct taaaattaag
    11461 agctatacct tatcataaaa ggtattttat gctttattta agcctacaat tctatattac
    11521 aattatcatc tagaccattt tcttatgtca aataaaccat atatattgtc tttaatagtt
    11581 ttatttgact ttttaagaaa gtatttactt ttgacttttt ttacgtttta caacatatac
    11641 gtattgtaat gttcagctca atttattata atctaaatga attgattaat aaaaattctg
    11701 aggttcgggt tttttatcaa ttcttttctg tttgtgtttg tgatgaaaca atcctttttc
    11761 ggtaggtttt gttatcatcg tcagcacaaa gagctaacgt tggtaccgtt agctcttttt
    11821 ttattttact ccgctcttac atagctatct aaatcctcca agtaaaacaa ttgattttct
    11881 cctatgcttg ttactccacg ccaaattgtt gttgttgctg ttgttggagt atttcttgtc
    11941 tgtgtaatca attcaaccat tttacctttt tgatttgttg tatagtaatc agcagtatag
    12001 ctagatattg gactattacc agtgcctacc atcgtgccag aaatatcaac aggactagac
    12061 tttactatct gaggaaattc tttttgtatt tccttcatat tcttcgtata tttttcccat
    12121 gaattccagt tgtaaaataa attaaagaat ttcctacttt gttccttgat ttcagcattc
    12181 cccaattctt tgtattgagc ttcctgcata gctttcactt tctggttatt cttgactact
    12241 tcgatttgtt tacttttaag gtcttccaat tctttgacat tattatcata agcatttttc
    12301 ttttgaaata gtaccacaca acaaaggatc acaattatgg aaagtattcc gatcgccttt
    12361 ttagcagtag tgatttcatg attttgcata aaaagcaccc ccagtcactt tcttatcatt
    12421 atttagtgtg atggttaatg tggtatcata cacttttttt ccattgtatt gaacttcaaa
    12481 atcggtcaat gcttcataca acccactact agatgtctta attccaaagt ttgttagctt
    12541 aaaagtaaaa ggcttatagt actcgatttt ttctttctca ctactcttat cagtaattgg
    12601 tggtttacct gttacaataa atagttttct caaaaattct tcacctgatt gatctgcaaa
    12661 cttattttga actgatccat tcaacatttc ttgaatatca ttttctaaat ctttccgtaa
    12721 tccatccacc tcatttggtt taacctcttt aaagaatgtc agcttatctt caatatgcac
    12781 actgtgtttt tcagcttcta tgtcctgatt ctgcttattg aagaatagga ttccaacaag
    12841 cagtgctaat aacataccag aaagcaaaga aatgataatt gtatctttca ctttactatt
    12901 catctatatc gtccttcatt ccaatttatt tttcgatttc aaatttagct tttcctacac
    12961 cgctaaagaa atgcacatta tctgatccgt ccactccatc aatcaaagtt accgaaactc
    13021 cccttgttgg agcttgggtt ggtggaacat taaagttagc acatagaaat gacccatctt
    13081 caaacaccgc tataactaca ccagtatgcc ctgcatcgcc aacagcccat aaccatccat
    13141 ctgaagcact aaacccataa cctacgattg gtttgttcgt tatttttgct cctgccgatt
    13201 tgtagctttc ccatacataa ccaccatttc cagtagtcgg ttgattccca tcgtataatt
    13261 ggctcatata agcccacgtc aactctgtac actgaccttg atatattccg ttaaaaggtg
    13321 aagttgacca atctggttct ttgaattttg gtagcgtgat cgctttccca tattttttca
    13381 aagcttcttc ataagtcata ttcgctatta cttgaccttt tttaccgtta atttttcctt
    13441 ctttcccaat tgatccagaa ccagaattag cgtcatccgt agttgcacag gcatcttcta
    13501 ctgttcctga tgctgaactt gatgaattat tcgatccatc tccggtactt gaacctccgg
    13561 atattttatt gatcttactt tcatcttttg caccgtccat acccttgctt ttcatgtact
    13621 tccagatttc ttttgcattt tcaccacgta cagctattac agctgattcg gaatcagctg
    13681 aaatttccca gtttttatga aaagttactg cattttctac gacatcatca gaagcattta
    13741 aagcataatc ttttaatatt cctacgaatc ccccatcacc tttgaacata aagtccaatt
    13801 gggcttctgt agtccaccaa gaaccgccat tatttttctt agaccaatca actaatgctg
    13861 tatgtcgccc agctgtccat tgaccatagc caatacccat cagaccatca gtaaattgtt
    13921 tgatgaagtc aacttctctc tgtccatctc cgtaataccc ttctactgct cgaggatcaa
    13981 tacctgattc ttgtttccag ttacctaaaa tacctgcaat actagcacct gataagccat
    14041 attctccatt catgtatttg taaatatctt caatattttt ggctacatca cctgttacag
    14101 cgccacttcc agtatcgttt ccatcgctat ctgttcctga cccactacct tcagtagtac
    14161 agttaaattg tgcatcataa gtagctatcc catcagcatg tatcacttgc ggatttattg
    14221 tgattacact cattgcaatc cctgtaaatt gcaccattaa cataagcaat gtggcaatct
    14281 ttctcttaga ttttttcaat aactacgccc ccaaaaagtg tactttttta tgttattatg
    14341 ataatataat aacataaatt aatagaaagg aaaagctaat aaatgactga taaaacaagt
    14401 gatttaccta aactagctcc aaaaacatct cgagtaaaga gcaaaaaaga tggaaaaaca
    14461 aaaaatgctg taaagaaaaa gtcagtaaaa gaaacatcct cgttactact tgatattcca
    14521 atgagttctt ttgatgacaa tacggacgaa acttctgtta aaattgcaac tcacgttgga
    14581 atgaacaatc aaaatgattc tttttacaac ttgttaaaca gcctaagaga gttggaagat
    14641 aaaaaaaatc aggtaatcgc atcaaaagct cctgatctca caaccgattt ggatgctgaa
    14701 attatccaaa tcaaacaaga tgagcgatca gttaaatcgc tactgaaaac ctactttgat
    14761 ggttttgata aagaactagc tccttatagc caagcaatcc taactaaacc aatacaagct
    14821 ccacatattt caatttccct acaaaaaaag agtgatgatt aaacaatcat tactcttttt
    14881 tagatttctg gtaagcgctg atcgcaaaaa ccatcaacaa tattgattca aaaaatatgt
    14941 tcattcgtgt ctcttttttc cattatagat ttcccactat tccattaaat cgaaagtcac
    15001 accgattact acaaagatta acgtgaccac attcaaaaac attaaaagat atgctgataa
    15061 tctcatcatt tttttagttc acttactggt aatctaaaca tttgaacgat tgcactggaa
    15121 ataccataat tagcaatgac aaacaaaaaa tacgcaataa tttcacttac acctagatag
    15181 atcagtaaaa taaataagtc acttaacatt gttagtttcc tcccagtata tgattttcat
    15241 ttttgggaag tcttttataa tccaattcct caaaaaaagg aaccatagtg tcactaatat
    15301 aggttttagt ttcaggtacc gccaaaattc cccaatcaag aatttcttcc catgatacat
    15361 catcaaattc taaaatatta tgatcaactg acctattacc aagttcatat ccaccaaaga
    15421 tcaccatatt atcaatcatg tatagaattt catcaggcga accatgagtt aatgtttcgt
    15481 cctcttcaag cagttcgttt aatctttgtt tgtattcctc tcttttcata cttttatccc
    15541 ttctgactca tttttttgaa ttttaacatt tttaacccaa gcatgactat ctttacatgc
    15601 tattaatttt tatctccatt ttgatatttc tgtttttttc tactcaccaa tcaaccgtaa
    15661 atcctcaata ttctcacctt caagcgaata tgaatatact tcgccgtaaa tattttctat
    15721 aactacgact tcttcttcaa agtctatgga ctgcacatca aaccattctc ctccgccaaa
    15781 ttcgtctatg attagtacct gcaatatttt atttactgcc atttcaattt ccccttatac
    15841 cattctatgt cttccaaact atttttcttc tttccatacc aaggtttcac gtatctcaaa
    15901 attttcttta aataagtgtt ccggtacttt tataattaac tcttttccta cttttatcag
    15961 atagtctttt tcacctaatg agatttgacc agtcggtaac tgtatagaaa ctcttttagt
    16021 tttattaaat tttttagttt tcaatacagt atacggtcta cagaatttct ctaagcgttc
    16081 aaatctttta ctatcgtatt ggaaataagc tttaggaaca gataaagtac aaaaactatc
    16141 ggtaacagca cttatgtctt ttttagaaat tatcccaact ttagaacttc taaggaaagt
    16201 tgctaatttc ttatatccac catgacttgt ttcaatatag atgctattgt attggctgtc
    16261 gtctatctca tttattgttt caaccttttt cctgacaggt gtttcaacga gttctcgaac
    16321 ctcttcttca ctgtacgcta atacaattaa ctcttcccca tttaccatct ttagaacata
    16381 cgcttgcttc ttttttcttg gcttattttg agattcaaca taaccagaca tggaatcttt
    16441 atacttccaa gcatgtactt ttttctctaa tttctgtatc tcttttttca gctgtagata
    16501 ttcagacatt ttttcattgt aacgttcgca atataaacaa tattcatctt ttccaaaatc
    16561 atgatcaagt ccttcttcaa tatgtttatt caacaaaccg gaaaaatact gatcatactg
    16621 cttctttcgt aacttcagct ctcgtaaatc ttcagacatt tcatttttcc tttccaatta
    16681 ttggtgccat ttcacaattt gggattgact aaacaccata attaatttgt aagctaacac
    16741 aattacaact gttggtaatt gtgttagtct ccatcaactt gtctggtggc tttttttatt
    16801 gcacaaattt catagctctc aaaaagcgct tggcttgaaa aggtgttatt ctacgaacct
    16861 tattcgccaa tttccaatct gataaagcaa tttgccattc gtgcatttca aaatccggca
    16921 tgatttcttt gatatactgt agctctttct tttctctttc ttttaagaaa cggctcatcg
    16981 agtttaacat tggaactaag gatgcgtagg tacttgaata acttccaatg cgtttcgtag
    17041 taattacata atcaataaaa acactattaa gaaaggtcaa gtttataaac tctgattcat
    17101 acactctaaa atttgcagtt aatcgcttcg ccttgtcacc ccaatagtca cttttgttgt
    17161 aggctttttc ccctcgaata tagcactctg tttctctttc gtaataatag tcatactttt
    17221 ttcatcatca aatctgagag tttttgatta tctttttcac tgtaaaataa tctactcgtt
    17281 aaacttttag gataatcttt gatagaatat gcatcccaaa caattctttg aacatattca
    17341 cgatctatat tttctctgac ttcctcactc caataatgtt gaccgtaata gcatttttct
    17401 attacgttgt tagcagaaac ttcaaaatag gattgttccc tttgtatcct cataccaatc
    17461 atcatagcgc aggaaataac tattaataac aatcctgttt ttaatctatt attccacatg
    17521 cagtttccac atccttatct tgataaaaat cctcttgacc ctctccaatt actgttttaa
    17581 atataaccac caaaacaaga tcatgctaag aaatccaaga gtatataaga tgatacattc
    17641 tttgttaatt atccaaaaaa ctctgatttc tttgagcttt tcacgcatta ggaaacacct
    17701 tgttatatct tttttgtatt tctttcaatt gttttttaaa actcataaac aactgttcat
    17761 gatctggtct aagcgttaga atatctaaat tatttatctt ttttaattgt gtaataaaat
    17821 ctattttgga ttttctttca atatcaaaac cgacaataat ctttcctgtt ggatcaacgc
    17881 atttcacttg aataactttg tcattttgcg atagctctaa tattgatgat ttttcttttg
    17941 cctcttccaa aagatgttct aacacttcgt taggtgttcc atgatagtaa taaatcatgg
    18001 tcttctcgaa aatttttatc ggatcaacac catattcttt tccgattcga taaaaaaatt
    18061 tcattggaat ttcttccgga tttctgttcc tgcttgacca gttagagatg gtttgataat
    18121 ttgcaaaatg gattcgttcc gctaatctct gaactgtggt ctctaaatct tcttctatta
    18181 attgttttac agcactaatg ttttgtgaca tttgctttct atcccttcaa attcgttgca
    18241 ttatcaattc taagatttca tgttttagtg catttctatc tacattttgc ttttttaact
    18301 ctgcaccata atatctcgca atcccttatc caagcataca attccttgat ctaatagatc
    18361 atattccgct ttttcaaacc ttcgctttgc ctgctcaaca agcaaaatga tattgtgatg
    18421 tatctgatta aactgccgat catattcctc taaagtttta gcatctaagt tagcaatatg
    18481 atcctctgct aatttttgtg ttaaaaataa ttcgactttc tcagatttca acatctgtat
    18541 tttttcatac tcttgttttg cttggatata gtcatctttc aagctagaca tctaactcat
    18601 ctccttgcct gtataaactg atacggactg tccaaaattg aataggttgg tgatgacttc
    18661 aagaagactt cacctttttc acatgaaaat aaaccaatta attcttcaat ttcatttacg
    18721 atcagatatt tattagttaa aattttccca ttttcttttt ctagctcgac aatcgcttga
    18781 ttccatgtat tttgcactaa atcttgaatc aatttcaatc tatccacttt tttcatctca
    18841 tacagggcaa tttcagcttg tttaatgcct tttaatttgc gaaaggcata tttgtattgt
    18901 gtgtaggtta aaaagcctgt gtagttatac atgggctttc ctgatgtctt gtagtcattt
    18961 aagcttggat atatttcagt tttccatttt tcttctgaac gttgatcaat aattcttaat
    19021 ttttcaggta aatcaatttc atagtattct tcaattttca gctcacgata aagtagatgt
    19081 ttttcctttt ctttttcaat tacaaaagaa ttatttccct tcagtttagc tatttcttca
    19141 cttgtgagac caagcctaat tttttctact tcacttctaa gttcacgaat acttgttgcc
    19201 tttaaccact tcccatttgc accattattg taaccggtaa gtaactgtat tttataaatt
    19261 ccttcaccat gaagcacctt atatatacca acctgattct tttgcggaat atgagcttca
    19321 tttgcactga tttctttcat tacgcattct cctttataat ctagttaatt agcttgtgtg
    19381 tttcgagtag ttgagtaaga acgtttgttt cagaaatcat ttatttgtta tcactta
//
LOCUS       DAZUMV010000041        18504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000041 DAZUMV010000000
VERSION     DAZUMV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.041"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8FK_001334"
     CDS             99..746
                     /locus_tag="ACG8FK_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5096950.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8FK_001335"
     CDS             1139..3784
                     /locus_tag="ACG8FK_001335"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS5096951.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8FK_001336"
     CDS             4094..5407
                     /locus_tag="ACG8FK_001336"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS5096952.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8FK_001337"
     CDS             5397..6050
                     /locus_tag="ACG8FK_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5096953.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8FK_001338"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8FK_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS5096954.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8FK_001339"
     CDS             6947..7147
                     /locus_tag="ACG8FK_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5096955.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG8FK_001340"
     CDS             complement(7401..9080)
                     /locus_tag="ACG8FK_001340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5096956.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG8FK_001341"
     CDS             complement(9082..9294)
                     /locus_tag="ACG8FK_001341"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS5096957.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8FK_001342"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8FK_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS5096958.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8FK_001343"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8FK_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS5096959.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG8FK_001344"
     CDS             13269..13466
                     /locus_tag="ACG8FK_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096960.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG8FK_001345"
     CDS             13619..14104
                     /locus_tag="ACG8FK_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS5096961.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8FK_001346"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8FK_001346"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS5096962.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACG8FK_001347"
     CDS             14981..15508
                     /locus_tag="ACG8FK_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096963.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACG8FK_001348"
     CDS             15599..16336
                     /locus_tag="ACG8FK_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5096964.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8FK_001349"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8FK_001349"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS5096965.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8FK_001350"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8FK_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS5096966.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAZUMV010000042        18344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000042 DAZUMV010000000
VERSION     DAZUMV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.042"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..1686
                     /locus_tag="ACG8FK_001351"
     CDS             25..1686
                     /locus_tag="ACG8FK_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5096967.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1756..2103)
                     /locus_tag="ACG8FK_001352"
     CDS             complement(1756..2103)
                     /locus_tag="ACG8FK_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS5096968.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2187..3128
                     /gene="opp3b"
                     /locus_tag="ACG8FK_001353"
     CDS             2187..3128
                     /gene="opp3b"
                     /locus_tag="ACG8FK_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5096969.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3129..4172
                     /locus_tag="ACG8FK_001354"
     CDS             3129..4172
                     /locus_tag="ACG8FK_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5096970.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4178..5248
                     /locus_tag="ACG8FK_001355"
     CDS             4178..5248
                     /locus_tag="ACG8FK_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096971.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5238..6191
                     /locus_tag="ACG8FK_001356"
     CDS             5238..6191
                     /locus_tag="ACG8FK_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096972.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6203..7213
                     /locus_tag="ACG8FK_001357"
     CDS             6203..7213
                     /locus_tag="ACG8FK_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5096973.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7498..8319)
                     /locus_tag="ACG8FK_001358"
     CDS             complement(7498..8319)
                     /locus_tag="ACG8FK_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5096974.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8416..8694)
                     /locus_tag="ACG8FK_001359"
     CDS             complement(8416..8694)
                     /locus_tag="ACG8FK_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096975.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8962..10971
                     /gene="metG"
                     /locus_tag="ACG8FK_001360"
     CDS             8962..10971
                     /gene="metG"
                     /locus_tag="ACG8FK_001360"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS5096976.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11450..12037)
                     /locus_tag="ACG8FK_001361"
     CDS             complement(11450..12037)
                     /locus_tag="ACG8FK_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096977.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12212..13534
                     /locus_tag="ACG8FK_001362"
     CDS             12212..13534
                     /locus_tag="ACG8FK_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5096978.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13689..14465
                     /locus_tag="ACG8FK_001363"
     CDS             13689..14465
                     /locus_tag="ACG8FK_001363"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5096979.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14458..15027
                     /gene="rnmV"
                     /locus_tag="ACG8FK_001364"
     CDS             14458..15027
                     /gene="rnmV"
                     /locus_tag="ACG8FK_001364"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS5096980.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15020..15904
                     /gene="rsmA"
                     /locus_tag="ACG8FK_001365"
     CDS             15020..15904
                     /gene="rsmA"
                     /locus_tag="ACG8FK_001365"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS5096981.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16355..16984
                     /locus_tag="ACG8FK_001366"
     CDS             16355..16984
                     /locus_tag="ACG8FK_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096982.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17108..18217)
                     /locus_tag="ACG8FK_001367"
     CDS             complement(17108..18217)
                     /locus_tag="ACG8FK_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5096983.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         5979 a         3311 c         3753 g         5301 t
ORIGIN      
        1 aattccaata aaggggtacg taaaatgaaa aagaaattta catttggttt ggttgcggta
       61 tgcggccttg tacttgctgg atgttatggc ggcagttcag ccacaagcag tagttcagca
      121 aaaagcggtt cagcagatgg aggaggcgta tttaacttag tcgtgcctca agaaatgcct
      181 acagctgact tatcagttgc aacagataca atcagtttta cagcattgaa taacgtttat
      241 gaaggaattt accgtcttga tgaagacagc aaaccacagc cggcaggagc tagcgagttg
      301 gcagaagtca gtgaagacgg attaacttac aaaatcaaat tacgagaaga cgcaaaatgg
      361 tcaaatggtg atcctgttac agcagcggat tacgtttatg gctggcaacg aacagtagac
      421 ccagctacag cttctgaata tgcttacctg tatgcgccag ttgaaaatgc agaagacatt
      481 acagctggaa agaaagataa atcagaacta gggatcaaag cagttggtga ctatgaatta
      541 gaaatcaaat tgacgaaaca aacaccatat ttccaatatc tgcttgcttt cccatcattt
      601 ttcccacaaa aacaatccgt agtggaagaa aatggggatg cctatgcatc tgcaagtgat
      661 aaagctgttt ataatggacc attcacatta gaagattttg atggaccagg aacagataca
      721 gaatggactt ataagaaaaa tgatcattac tgggacaaag atacagtaaa actttctgaa
      781 atcaaagtca gtgtagtaaa agaatcttct actgcattga acttattcaa agatggccaa
      841 gcagatgacg tgatcttgtc tggtgagtta gcacagcaaa acgcaaacga tccagcttat
      901 acttctgtta aagaagcgcg gacaagctat atcgaattca atcaacgtga aaagaattca
      961 ccattcaaca atgtgaacct aagaaaagct atttcttatt ctatcgatcg tgaagcttta
     1021 gtaaaacaag tactaggtga cggatcagtt gtctctacag gattgatccc atcagatatg
     1081 gtaaaaagtc caacaacgaa cgaagacttt gcaaaagaag ctggaaaatt agtttcttat
     1141 gacaaagata aagcaaaaga atattgggaa aaagcgaaaa aagaattggg cgttgattct
     1201 ttagaatttg atttgatggc ttctgatgat gattcatcga aaaaagtaat cgaatatgtt
     1261 caaaactcaa tccaagaaaa cttggatggc gtaacagtca aaccaacacc tgttccattc
     1321 tctgtacgtc ttgaccgctc aacttcgggt gattttgacg cagtactagg cggatgggct
     1381 gctgactatg cagacccaag cagctttact gatttgtttg tcacaggtaa ttcatacaac
     1441 cgtggtcgct ggagcaatgc agaatatgac gaagcagtta aagcagcttc atcaacagac
     1501 gctggcgatg aacaagcccg ttgggatgat ttccaaaaag caatcaagat catctctgat
     1561 gatatgggtg tcgttccggt gtaccaaaaa gcagaaggtc acctagtgaa tgaaaaaatc
     1621 aaaggtatcg ttcaccatgc ggcaggcgct tcatgggatt acaaatggac atacgttgaa
     1681 gaataatcgt taataccgtg agcatgagaa aaaacgaggc tgcggcaaat gtcgcaacct
     1741 cgttctcata ttcacttatc ctaacaaaga aaaaagcatg aaaatagcag aaagagcaat
     1801 tagtattccg gcaatgatca gccaaaagct gtacataccg tagctggcag acgagagcga
     1861 ggaaaattct ggttttttct ttcggttcct tgttcgtgca agatggaccg atcgctgaaa
     1921 atgatcgaag aaacccgaag aaaagaccca taaaaaaccg ccgataatca gaaacggcaa
     1981 tgaccataag aatagatcat tagataattg aatgagtgta aaggaatggt tgatgagatt
     2041 ttttattaaa ataaccagaa tcagtagaaa agctactgcg taggggatag attttttttt
     2101 catttgattg ctcctttatt tttggttcat tatctaataa tgtagcgaaa catctgtcag
     2161 aagtcaaaag ctagggggaa acattagtga atagttatat caaatatgta ttaaaacgtg
     2221 tctttttcat gatcatcacg ctttggttaa ttgcaacaat cacctttttc ttgatgcaat
     2281 tgctgccggg cacgccatat acgaatcagg aacgtttgag tcctgaaaca atcgagatgc
     2341 taaataaaca agtcggtttg gataaaccag ttatcgtaca atatgggatt tatctttcta
     2401 atttgcttca aggagatttt ggaatttcct tccaatttaa aaaccagcca gtcgcacatt
     2461 tgttagctgg aagaatcgga ccatccttgc agttagggtt acaggcaatt atctttggaa
     2521 cagtcctagg gacgatttta ggaacgatct ctgccatgaa gcaaaataca tgggcagata
     2581 catcttctac actcgtagcg atattaggtc gttccattcc aaactttgta tttgctgttt
     2641 tattacaata tatctttgca atcaaattac aagttttgcc aatcgcaaaa tgggatggct
     2701 ttgtgtacac aatcttacca acaattgcgt tggccatgtc gccgcttgct gactctgctc
     2761 ggtttatccg tacggaaatg gtggaagtac tgcatagtga ttatgtggaa ttagcaaaag
     2821 caaaaggatt gagccgttgg gaaatcgcct ttaaacatgg tcttcgaaat agtttgattc
     2881 cattgatgac tttattagga ccgttagctg tagcattgat gaccggatca ctggtggtag
     2941 aaaatatctt tgctattcct ggtatcgggg aacaattcgt taagtcaatc acaacaaatg
     3001 attatcctac gatcatggct gtaacgatcc tttactcatt tatgctgatt tttgttattt
     3061 tgatcgtgga tctattgtac ggtctagttg atccaagaat tcgtgtttca gaagggagcc
     3121 gaggataaat ggaaatgata ccaaagcgtt atgaaacagt tgcagatatc cctgcggatg
     3181 aatttcttcc attacaaaaa aataccgaag aagaacgtga acagattact gcaccttctt
     3241 tgaattttat ccaagattca tggcgccgtt taaagaaaaa caaagctgcc gttatttcaa
     3301 tggttttact attggtcatc attttgatct ctgtgatcac aatctttgtt tctcctcatg
     3361 atccaactgc ccaaaacgtg gcttatatca atttgcctcc tcgtattccg ggaatcaata
     3421 tcgatggatt gaatggaaaa gcaatggttg gtggtgaact tgttgataaa tatgctcaag
     3481 caaacgtccc ttcaaacgta aatttctttt taggaacaga cggtttggga cgtgatgtat
     3541 taagccgctt atttatggga atgagaatct cgttgctgat cgcatttatt gctgcattac
     3601 tagatgtaac gattggtgta gcttacgggt tggtttctgg tttgttaggc ggacgtgtcg
     3661 ataacgtgat gcaacgtttt ttggaaatct tatcaggaat tccaaacctc gttgtcatga
     3721 ttttgatgtt ggtcgttttt gaaccaggaa ttttttcaat cgtggctgct atggccatca
     3781 caaactggat accaatggca cgtatcgttc gtgcacaaac aatgaaatta aaagatcaag
     3841 aatatgtttt agctggattg acattaggtg aatcaagaat gaaaattgca ttcaaacata
     3901 ttttgccgaa tatctctagt gtcattatta ttcaaatgat gtttagtatt ccatctgcta
     3961 ttttcttcga agccttttta agctttatcg gtttaggtct tacgcctccg tcagcttcat
     4021 taggaacgat gttgagcgaa gggtacaaga cattcctgta tctgccttat ctattatgga
     4081 ttccggcagt aactttatcg gttatcatga ttggctttaa cttacttgct gatggattgc
     4141 gtgatgcctt tgatcctaaa atgaaagagt gaagacgatg acgaaaattt tagaagtgaa
     4201 agacttagaa atctcttttg atacgtatgc tggaaaggta cgagcgattc gcggtgtcaa
     4261 ttttcatttg aataaaggag aaacattggc gatcgttggg gaatctggaa gtggaaaatc
     4321 agtgacgacc agaagcatca tgcgcttgct gtcaagcaac gccaatatag atgctggtca
     4381 aatcctcttt aaaggtaacg atatcgttgg aaaatcagaa agagagatgc aaaaaatccg
     4441 tggtaaagaa atagcgatga ttttccaaga tccaatgacc tctttagatc cgacgatgcc
     4501 cattgggaag caagtcgctg agtcccttag aaagcataat aaagtttcaa aaaaagaggg
     4561 acaaaaagct gcattagaat tgcttaaatt agttggaata cccgaagctg agaaacgtat
     4621 caacagttat ccgcatcagt tctcaggagg tcagcgtcaa cggatcgtga tcgcgattgc
     4681 actggtatgt tatccggaaa tcctgattgc tgatgaacct acaacagcgc ttgatgtgac
     4741 tattcaagca cagattttgg agctgctgaa agaaattcag acaaaaatcg atacatcaat
     4801 tatctttatc actcatgacc ttggtgtagt agcgaatgtc gctgatcgtg tcgctgtcat
     4861 gtatggagga agaatcgttg aagttggaac atcggaagaa attttctaca acccgcaaca
     4921 tccctatact tggggactgc ttggttcaat gccgacatta gatagtgcga acgatcgatt
     4981 gtatgctatt cctggttctc caccggattt attgaacccg ccaaaaggag acgcattcta
     5041 tccaagaaat gaattcgcga tgaaaatcga tgcagaacaa gaaccaccat tttttgaact
     5101 atcaaaaaca cacaaggcag caacttggct acttgctccg caggcaccca aagtgacacc
     5161 gccagaagaa atccaacgtc gctgggctat ctttaaagag aaacaaaaac atacttacgg
     5221 tcaaggagga attgctgatg ccaaataaac tacttgaagt taaaggattg aaacagtatt
     5281 tcaatgtagg aaaaaagaat gaagtacatg ctgtaaatga catcagcttt cacatttatg
     5341 aaggagaaac attcggttta gttggagaat ctggaagcgg aaaatccact actggccgta
     5401 cgatcattcg cttgaatgaa cctactgatg gtgagatatt attcgacggt caagatataa
     5461 cgaagatcaa agataaaaaa ggtttgacga aattccggca tgatgttcag atgatctttc
     5521 aagatccata cgcttcgttg aatcctcgta tgaaagtcag agatatcatt gccgaaggaa
     5581 ttgatgttaa tggactagct aaatcatctg aagagcgtgc aaaaaaagtc gatgatctat
     5641 tgagaactgt tggattgaat ccaagccacg gtactcgtta tcctcatgaa ttttcaggtg
     5701 ggcaacgcca gcgtattggt attgcacgag cattggcagt aaaaccgcgt tttatcattt
     5761 gtgatgaacc aatttctgca ctagatgtgt cgatccaagc ccaagtggtc aatttgttgc
     5821 aagatctcca aaaagaacat caattgacct atctatttat tgcacatgat ttgtcgatgg
     5881 ttaaacatat tagtgaccga atcggtgtaa tgcataacgg cttgttatta gagatgggaa
     5941 caagtgatga aatctataat cacggtgttc atccttatac agaaagtttg ttatctgcta
     6001 ttcctctacc agatccagac catgagagaa aacgcagaag aatcaaatat caacctgaac
     6061 cagatgacgg acaagtacgt caacttcgtg aaatcgcacc agaacatttt gtctatgcaa
     6121 ctgagcagga agtcccatat tacgcaaaaa aactaaaacg acaaaaggaa tctcttctgg
     6181 ttgctaacta ggagggacat agatgctaaa agcttttaaa ttccgtatgt atccgacaga
     6241 agaacaaaaa cagcaattga ttcgtacttt tggatgtgct cgatttacct ataaccattt
     6301 gctgaaaaag cggcagaaaa gttggcaaca aacaggagta gccaattttt cgctcacacc
     6361 cgctacatta aaaaaagaat acccattctt aaaagaagta gacagtcttg ctttagcaaa
     6421 tgcacagctt aatttggatc gggcttttcg caattatttc aagggacgtg ccagcttccc
     6481 aaagcttaaa acgaaaaaaa gtatgtggca atcttatacg accaacaacc aaacccgtac
     6541 gatctatctg gaaaatggct acttaaagtt accaaaacaa aaagaactaa tcaagataaa
     6601 tcagcatcgg ccagttgaag gatcgattcg ctccgctacg atttctgctc gatataatga
     6661 agaattttat gtcgcactac tttgtgatgt ttcgccagta aaaaaagaat ctttagctaa
     6721 atggatcggg atcgcctatc atccgaagac attgatccaa acatctcgac ctctagaagt
     6781 aactttacca aaatttcacc agacagaaga aaagctgcaa catgcacagc ggaaattgaa
     6841 tgtgaaagtc cgctccgctc accatagaaa gatcaggtta gatcaggcaa gcaattatca
     6901 gaaacaaaag agaaaagtaa tggatcttta tctgaaacaa aagaaccagc gagaagatta
     6961 tcttgaacaa ttatctggga aattggtcaa acaatatgac tacttatttg tagaatcttt
     7021 tccaaaagaa gaggcacatg ctgatttttc tattcacgac tggcataaat taataacaaa
     7081 attgcgttat aagtcccaat ggtataataa aaaatttctt ttgatcaata ccgacggagc
     7141 agaagaaagc aattccgtaa gaaagagcca agtagtagaa aaaatgggca gacattcggt
     7201 tatcaaggga taaaaaatga atgaaactga taggagcgtt agggttagcc ttgttaataa
     7261 aaagtcttaa tgacttttgt ttctaggaat ttttcttttg aagaaaagct cactaattat
     7321 gacaaaccaa taaaacaata gcagaaaaag ttcgtacgag ctttttctgc tattgttaat
     7381 tttttattca gaaagtttac gaatacatca aaattctgaa acaaaaaacg taactcacaa
     7441 agagttgttt ttgcgttttt tcttgaatga ttaggtctga atcttttgct cacaacctta
     7501 ttcatggtgc cccggcttga gctgatagat ttttgaagga cggccgattc ctacaggacg
     7561 ttcaccgatc tcatcgaaat attggagcat agctttctta aaattggaat gatcgatcgt
     7621 tttataatcg atccctaaga atttagcaaa tacttttcga gcttccgtaa tcgtgaaatc
     7681 tttacctaaa acttgtagaa cttgcggctc gtgttccatt ttattgacta cacggttgaa
     7741 tgctttcaag atgatttgac tatggtcaaa agccaaagta tctttgccag aagactctcc
     7801 agtgactaaa tcaagcgaga tagccacttc tccgctagat aaatggatct tgttactatg
     7861 acgttctagt gtgaaccagc gtacttcctt ggcatcatct ccggcgatta gcggttcttc
     7921 tccaataaag gctaagtagc tgactgtgac gacccagcct cgcggatctc gattaggcgt
     7981 actgaatgta tgcagttgct caatattttc ttttgaaata accacaccag tttcttcctt
     8041 tgtttctctt aagacgcttt ctccagttga ctcatccttt tggacaaagc caccaggcaa
     8101 tgcccatgaa tttcgataag gatgcccttt tctttggatc aacaaaactt tcagctgatc
     8161 ttcttctttg ttatagcaca tcagtacgat atcgacagtc aaagagggct tgtcatattc
     8221 tggacgttcc tgtttttgat accagttaag aaattcttct tcacttgctt tatgttcata
     8281 atataatttt tcttcttctt tagaagcaaa acggttcatc tgaccacctc cattgacagt
     8341 aaaagtactt tttaccttat tgtagcagac tctgaaaaaa aaccaaggat ttacttgaaa
     8401 aatctaggtt atcatttaaa tatgaaaaat aaatggaagt atgagattga ataaaaagaa
     8461 aagcagataa aacggcaata ctttataccg ctcttcacga atggcaaaga agccacaaat
     8521 aacgatatat acgtcaatga taaaacgcaa aatccaagct gctgtcataa aactgttcaa
     8581 atgcccatgg aggttaaaat aaccatacat acgaatataa aaatagctga atattaaatg
     8641 tgctaatgaa agaaaagcaa tactaagtgt aaaaaaaaga tgtttcttct gcatgattgt
     8701 tccagtccct tactaagata gatagtcttt attataccac gtgtgagggg agaaataaaa
     8761 agccgttttt tagtagatat gaaacaaaac aaaaacccgc atttattcaa cctattcatc
     8821 cttttaaagg atagattgaa cgtagataga agtcgtttat ccagtctttt tcattttgaa
     8881 aaaaagctaa cagcatgctt tttttatggt atactagcaa ggaaacgaat tcgcatcatg
     8941 cgataaagga ggaagagccc cgtggcagaa aaggaaactt tttatatcac tacgccaatc
     9001 tattatccaa gcggcaaact tcatatcggc aactcgtata ctacgattgc atgtgacgca
     9061 attgctcgtt ataaacggtt gatgggcttt gatgtgtttt atctcactgg agtggatgaa
     9121 catggtcaaa aaatcgaaac aaaagcagaa gaactaggtg taaaaccgca agaatatgta
     9181 gataaaatgg cggctgatgt caagaaatta tggaaaacgc ttgatatcag ttatgataaa
     9241 ttcattcgca cgacagatga ttaccataaa gcagctgttc aaaaaatatt tgatcgttta
     9301 ttagaacagg gagatattta cttaggtgaa tatgaaggct ggtactctgt ttctgacgaa
     9361 gaattcttta cagaaacaca acttgcagaa gtgtacaaag atgacgaagg taacgtgatc
     9421 ggtggtaaag cgccaagtgg acacgaagta gaattggtaa aagaagaatc atacttcttc
     9481 cgcatgagca aatacgcaga ccgtctattg gaatactatg aagaacatcc cgaattcatc
     9541 cagccagaat ctcgtaaaaa cgaaatgatc aacaacttta tcaaaccagg attagaggat
     9601 cttgcagttt cacgaacaac ttttacttgg ggagtaccag tcaagagtga tccgaaacat
     9661 gtggtgtatg tatggatcga tgcattatcc aattatatta ctgcactagg atatggctcc
     9721 gaagatgaaa gtctattcga aaaatactgg cctgcagatg ttcagatggt cggtaaagaa
     9781 atcgttcgct ttcatacgat ctattggcca atcatgctga tggcccttga tttgcctcta
     9841 cctaaaaaag tatttgggca tggctggcta ttgatgaaag acggaaaaat gtctaaatca
     9901 aaaggaaatg tcgtttatcc tgaaatgctt gtggaacgtt atggtttaga tgcactccgt
     9961 tattatttgc ttcgtgctgt tccgtttggg tcagatggtg tctttacgcc agaagatttt
    10021 gtttcccgtg tcaattatga tctggcaaat gatctaggga acttattgaa tcgaacgatt
    10081 gctatgatca acaaatattg tgatggactc gttcctgact atgcctcact tgtcactcca
    10141 tttgatagtg aactgtctac gacggctgct aacgttgtcg gacgttacca tgatgcaatg
    10201 gaaaaaatgg aattcaatac agcattggct gaaatctggg tgctgatttc tcgtgcaaac
    10261 aaatacattg atgaaacaga accatgggta ttagcaaaag acgaagaaaa gaaagctgaa
    10321 ttgaatagtg tcatgatcca tttagctgaa tctttgcgta tcgcagcgat attacttcag
    10381 ccaatcatga cagaaacgcc agagaagatc ttcaatcaac taggattgga ctctgaaaca
    10441 atgaatcttg aaggattaca ctttggcgaa tttccttcag gaacaaaagt agttgcaaaa
    10501 ggtacaccaa tcttcccacg tttagatatg gaagaagaag ttgcttttat tcaagaaaaa
    10561 atgtctgaag gtactcaaac aaacgaagat acagtcaaat gggatccaga agaaacagaa
    10621 cttgtttcaa cgaaagaaaa acaaatcaaa tttgatgtat ttgaaaaagt agaactaaaa
    10681 gtagcagaag tcatcaactg tcaaaaagta gaaggggcag ataagttgct gcaattccga
    10741 ctagatgctg gagacagcca agaccgtcaa atcttgtcag gaatcgctga attttatcct
    10801 gatccaagcg aattgatcgg gaaaaaagtc gtcattgtag caaatctgaa accacgaaag
    10861 atgcgtggac aaatcagtca aggaatgatc ctttctgcag aagcaccaga tggctcttta
    10921 caagtaatcg aagcaccaaa atccatgcca aatggctcag aaatcgcata gaaattcaaa
    10981 gggggtatga cactagttgt gtcatacccc ctttttccga aatttgagaa gcaattttcg
    11041 taaattcctt cttattactt gaagccaatc atataataca gaaacagaga actatccaaa
    11101 ataaatccta gaaaaagaag tacgagcttc gctgattttt gttaatttag aataatacta
    11161 ataggaatcg taaaaacaga aaaaaagcat aatgataaag ggtcgggcag attttaattg
    11221 attcatctgg tactcttttt tattgtcctt acattcataa agtttgaatg caatgacttt
    11281 attacatcta tatttatatc taacagtagc gttgatgtgt taattataag ttctatcact
    11341 ttatgttcaa ttagtttgtg ctcttatatg tgtgattgaa aatatagata aaaaaacaat
    11401 catcggcttt gacttcgatg attgttttct ttattttgta aaaatcctat cacttgattt
    11461 tatggtaacc ttttattttt gtcttcaaac ggcttggtaa gagagcagta agcagtgcaa
    11521 atgggatcga aaagacatac cagaaaatcg tacgtccgat accataatgt tgatagagct
    11581 ttgtcaacat cacgtgtaac aagatcaacc ctaaaagcaa ggagccagct ataaaccaat
    11641 gattatccca atgcatgact tcactaagtg ccatcactcc aaaaggatag aaataaaaat
    11701 agatcccgac taaggtcatc cgccagttct ttgtaaatgt tccgatattc aataaaaaga
    11761 aaatcgctga aagcaaaata ccgataggcg gaaaaagccc aaccaaaaag gaatacaaca
    11821 tcccccacaa gatcatcaca ggacctttga tcaatgcaac gattgcgatg aacaaagtca
    11881 gcaatattag tttttgtgat aacgttgcaa cagtcattaa tccaaataaa attaatgtga
    11941 accaatgcca atgggactgc tgttgttctt tacgggaaaa atcgacccgg ttggataaat
    12001 aatctgctaa tttcggataa gaacgcttaa aatccataaa ctgttcctcc ttcgtggaaa
    12061 aggtaggttt agtatacgaa aaataaaaaa gaaatacatc cgtcttaaga gggagttttt
    12121 cactgaaagt ggagaaaaaa gaatggtaaa ttaaaatatt ctcagttata ctgaaaatta
    12181 taccaaattt caaagaagaa agaggatggg aatgaaaggc aaactaaaac gagaaatcaa
    12241 tctgtttggc gcattagcta ccgttatggg aaccgtgatc ggtgcaggcg tcttcttcaa
    12301 aactgctgct gttactgcta gtactcagtc tacaagtttg acattgctag cttggctttt
    12361 gggtggtttc ttaacgatct gtgctggatt gacagtggct gaattagcta cagcaatccc
    12421 ggaaactggt ggagctgtaa aatatattga agctgcgtat ggaaaattgc ccagcttttt
    12481 attaggatgg gcgcaaagct tgatttactt tcctgctaat attgctgcat tatcgattat
    12541 ctttgcgaca caattgacga acttattaca attatctacc gattatcttt tgttgattgc
    12601 agtgatcacc gctgtttctg tgacagggtt gaatttgtta gggacaaaag tcggtgcttc
    12661 tgtacaatca gtgacgttga ttgttaaatt aataccaatt gctgtgattg tcatatgggg
    12721 attattgacc ccaggacaag gaactgtcca gttatttcca atcgaagctg gaaaagatgt
    12781 tacttttgtc gaaggattga gcagtgcgtt gttagcaacg ttgtttgctt atgacggctg
    12841 gttaggtgtc ggtgcaatgg caggggaaat gaaacggccg gaaaaagatt tgcctaaagc
    12901 aattattctt ggtttgagtt ttgtaacagt cgtttatcta ttgatcaatt ttgttttttt
    12961 gaaaactttg ccgattgatc atcttgccgg taatttgaat gcagcttctg aagcatcaga
    13021 cgtcattttt ggaggaatcg gtggaaagct ggtcactatt gggatcttga tctcggtata
    13081 cggtgcattg aacggttata cactaactgg gatccgcgtg ccttatgcga tggctttaga
    13141 agacgagcta cctttcagta aacagctgac aaagctttca aagaagttta cggttcctta
    13201 tgttcctgcg gttttccagt tagcagtagc ttgtattatg atgagccttg gttctttcga
    13261 ctttttaaca gacatgttga tttttgtgat gtggctattc agtttactga tttttattgg
    13321 tgtctttatt ttaagaaaaa aagcgccgga attgccacgt ccatataaag taccacttta
    13381 tccaatcgtt ccgattattg cgattctcgg tgctattttc attttaggta tgacgatgat
    13441 gacgcaaacg aaattagcat tgatcggtat aggtgttacc ttgataggta tcccagtcta
    13501 ttatcaaaaa aagaaaaaac gctcagaaga gtaaaatatt tcaacaacag acagaaaaag
    13561 tacgaactag gtcctctaga tcttcgtgct ttttctattt caataaaaca agttcttcta
    13621 ttttgatttt tgctacttct gcaagtagtg cttatgtgct aaagtaagaa tgaaaaggag
    13681 agataaacat gatatttgat tctcataccc atttaaatgc tgaacagttc aatgaagata
    13741 ttccagaaac gattcagcga gcacaagaaa tgggggtaac gaaaatggcg gtcgtgggct
    13801 tcgatacgcc aacgattgaa aagtctttgc aattaagcca tgactatcca aatatataca
    13861 gtattatcgg ctggcatccg acagaggctg gcagttatac gaaagaaatc gaaaataaat
    13921 tacaagaaca attgacttta ccaaaggttg tagcattagg ggaaattggc ttggattatc
    13981 attggatgga agacccgaaa gaagtccaag ataaagtatt tcgcagacag attgctatcg
    14041 caaaagaaat gaacctacca atcagtattc atactagaga agcacttgaa gacacgtatc
    14101 aaatcctaaa agaagaagat gttcgagata tcggtggaat catgcatagt ttcagtggtg
    14161 attatgaatg ggcaaaacga tttcttgact tagggatgca tatttcattt agcggtgtcg
    14221 tcactttcaa aaaagcatta gacgtccaag aggctgcagc aaatgtacca atcgaacgat
    14281 tacttgtgga aacagatgcc ccttatctgg caccagttcc ttttagagga aagcgaaatg
    14341 aaccaggcta tactcgttat acagtagaaa agattgctga acttcggaat cttccgacag
    14401 aagaagtggc tgcccaaaca tggacaaatg cgcaccgatt atttggtcta agcgaacatg
    14461 gataagatga agatccaaga gattatagtc gttgaaggaa aagacgatac aagaaggctg
    14521 cgggaagtag ttgatgcaga tacgatagaa acaatagggt ctgcaatcaa tgaagagatc
    14581 ctgatgcaaa ttgaacatgc ccaagagact cgtggagtga tcgtttttac cgatcctgat
    14641 ttttcaggag aaaaaataag aaaaatcatt atggaagctg ttcctcaggc aaaacatgct
    14701 tttttgccgc gcacacaagc taaaccaaag aaaaagggaa gccttggtgt cgagcatgca
    14761 agtgatgctg ctattttaga agcattgaaa aaggtcgtga cgccagacat ttcccaaacg
    14821 gctgtttctg aaatcactag acaagacctt ttagactatg gtttaatagc tggggcacaa
    14881 gcaaaagaaa aacgagaaaa actaggtgac gagttgcgca tcggttacac gaacggcaaa
    14941 caattggaaa aacgtttgaa gatgtttcgt attacgccga aagaactcgc ccaagcaatg
    15001 aaaatagtgg aggaacaaca tgagtgaata tcgtgaaatt gcgacaccct ctagaacaaa
    15061 agagatttta ttgaaacatg gtttttcatt taaaaaaagt ttaggacaaa attttctgac
    15121 tgaaccaaat attttaagga aaatcgtaga aactgcgcaa atagatgaac atacgaacgt
    15181 gatcgaagtt ggaccaggaa tcggtgcatt gacggaacaa ttagccaagc atgcaaaaca
    15241 agtcgtagca tttgagatcg atgatcgttt gatccctgta ttggctgata cgatgcaacc
    15301 ctatgacaat gtgactattg tacatcaaga tattttaaag accgatttgt caacagctgt
    15361 tagagaaaca tttcatgagg agcttccgtt aaaagtcgta gcgaatttac cttactatat
    15421 tacgacaccg atcatgatgc attttcttga atcagatttg gttgtggatg aactagtcgt
    15481 catgatgcaa aaagaagtag ctgatcgtat ctctgctgaa ccaggaacga aagcttatgg
    15541 ttcattatcg attgctgttc aatattatat ggaagccagt ttagcattca tcgtaccaaa
    15601 aacagtattt gtccctcagc caaatgtaga ttcagcgatt ttgaaactga caagaagaga
    15661 tataccggca gtcgaagtga cagacgaaaa agcatttttc cgattgacaa aagcggcatt
    15721 tcagcaaaga agaaaaacgc tttggaataa cttgcagcat tcgtatggca aagatgacca
    15781 gacaaaagca tggttagcaa aaagcttgga aacagctgga atcgatccaa aacgtcgtgg
    15841 ggagactttg tcattacaag aattcgccgc attaagcaat gcgatgtcag aaaatatgca
    15901 gtaaaaaaag aggttgtgac agaagttgtg aaaaagctgt cctgcatcaa gtaataagaa
    15961 cagccaaaca aaaatagttc ctcatgtttt tcgtttgttt gggcttatta ccgcagatgc
    16021 aagcttttga acaccgttta agaaagaggt tgtgggcctg ccgtttaact ctaagtcaca
    16081 aggaacaatt tcaaaaaaca gcttctcatg tttttgagtt agttggtctt attacttgat
    16141 gcaagctttt gaccactatt atgccgaaca aggagatgtg acacaaattt tgtcacatct
    16201 cctatttttg tttcttgatc aaaaaattct tatacttgat actcacactc attttgtatg
    16261 gatttagaaa agaatatgtg tcttgacagt tattagtaag tgagttatac taagtcgaca
    16321 tttatgtgtc aagacatatt aggagagaga aaaaatgaga aaaaattcag ttcgaaaact
    16381 aacgatttct gctttattga ttgggatggg ggtcattatc ccaatggtca tgcctaaaat
    16441 catgattcca ccagcttcct ttactttggc gtctcatgtg ccattattta ttgcgatgtt
    16501 ttttacacca ggagttgcag tggctgttgc gctaggtacg acatttggtt tctttttaac
    16561 aacgccggtt atcatcgcat tgcgagcatt gtcacatctt gtgtttgcgt tgattggggc
    16621 ttggtacttg caaaagcatc cgagtatcgt tttaaaaaat ggacagttta cttttgctaa
    16681 ttggcgtttc caagggttca attttgtgat cggtgtgatt cattctttag cagaaatgct
    16741 agtagtcagc atcttttact ttattggtaa tatgcctgaa acatattatt ctcaaggata
    16801 tttttataca gtattcatct tgatggggat tggtgggctt atccacagtt tagttgatta
    16861 taatattgct tattttatag caggaacact cagcaaatat tttgatatcc ccgtatttac
    16921 ggcagcaaaa aaagaaaatc ggaaagagtc gattcaaggg tacgcgaaga aaacgacata
    16981 ttagacatga tctctcaaaa aaaaaactct catctagtaa tggataataa atcctctaag
    17041 atgagagttt tttgtcctaa aaagagctgc accaacatct tctggcacag ctctaacagg
    17101 gggcaattta tggaagtttg atgacttgtt ctgtttcttt atcaaagaag tggcctttgc
    17161 tgatgttgaa ggcaaggtcg actatttcgc ctggtttatg gaaatctctt gcatctactt
    17221 ttgagatgaa ctcagtagaa ccagtttttg tgtaaagcat cgtttcagct ccaagtaatt
    17281 cggaaacgac aacttctgat ttcactacgg cttcaggtga agcttcaagg gctacttgtt
    17341 cactgtggat gtcttctgga cgaataccga agataacttc ttttccttca taaccttttt
    17401 caactaatac tttgtttttt ccttcaggaa tacgaagctt aagcccatgt ccatctgtga
    17461 taacaccatt tttcaatgtc acattaaaga agttcatggc aggagatcca ataaagccgg
    17521 caacgaacat attgacgggt gtatcataaa cttctttcgg tgtaccgatt tgttggataa
    17581 atccgtcttt catgataacg attcggtcag ccatagtcat tgcttctgtc tgatcgtgag
    17641 taacatagat ggtcgttgtt tcaagacgct ggtgaagttt ggcaatctca gcacgcatgg
    17701 ctacacgtaa tttagcatct aaatttgata atggctcatc cattaagaag acttttgcat
    17761 cccgaacgat tgcccgtccc aatgccacac gttggcgttg tccaccagat aaagcagctg
    17821 gttttcgttt caagtattct gtcagaccta agatctctgc tgcattgtcc acacgttttt
    17881 tgatgtctgc tttatcatat ttacgtaatt tcaagccaaa agccatatta tcaaaaacag
    17941 tcatatgagg atacaatgcg tagttttgga aaaccatggc aatatcacgg tctttaggtg
    18001 ccacgtcatt catgactttt ccgccgatat ttagttctcc ttcagagata tcttccagtc
    18061 ccgcgatcat tcgcaaagta gttgactttc cacaaccgga aggaccaaca aaaacgataa
    18121 attcacggtc tttgattgat agattaaaat cagttacaga ataattttct gcattgtcat
    18181 attttttata tacgtttttc agagccattt ctaccataat acgttccaca ctttcttttt
    18241 tagttttatt aactatttaa agtataaatg aaagcgttaa ctaacgtcaa tgtcatgttg
    18301 aacaagaatt ttttcttttt tttgtcaatg tgctagtttt aagc
//
LOCUS       DAZUMV010000043        17920 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000043 DAZUMV010000000
VERSION     DAZUMV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.043"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            299..790
                     /gene="nrdR"
                     /locus_tag="ACG8FK_001368"
     CDS             299..790
                     /gene="nrdR"
                     /locus_tag="ACG8FK_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS5096984.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            813..2186
                     /locus_tag="ACG8FK_001369"
     CDS             813..2186
                     /locus_tag="ACG8FK_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5096985.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            2186..3112
                     /gene="dnaI"
                     /locus_tag="ACG8FK_001370"
     CDS             2186..3112
                     /gene="dnaI"
                     /locus_tag="ACG8FK_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS5096986.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            3241..3681
                     /locus_tag="ACG8FK_001371"
     CDS             3241..3681
                     /locus_tag="ACG8FK_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS5096987.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            3825..4526
                     /locus_tag="ACG8FK_001372"
     CDS             3825..4526
                     /locus_tag="ACG8FK_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS5096988.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            4523..5644
                     /locus_tag="ACG8FK_001373"
     CDS             4523..5644
                     /locus_tag="ACG8FK_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS5096989.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            5659..6891
                     /gene="pepT"
                     /locus_tag="ACG8FK_001374"
     CDS             5659..6891
                     /gene="pepT"
                     /locus_tag="ACG8FK_001374"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS5096990.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            7023..7931
                     /locus_tag="ACG8FK_001375"
     CDS             7023..7931
                     /locus_tag="ACG8FK_001375"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5096991.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            7966..8238
                     /locus_tag="ACG8FK_001376"
     CDS             7966..8238
                     /locus_tag="ACG8FK_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS5096992.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            8249..9295
                     /locus_tag="ACG8FK_001377"
     CDS             8249..9295
                     /locus_tag="ACG8FK_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS5096993.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            9546..12155
                     /gene="clpB"
                     /locus_tag="ACG8FK_001378"
     CDS             9546..12155
                     /gene="clpB"
                     /locus_tag="ACG8FK_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS5096994.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(12306..12992)
                     /locus_tag="ACG8FK_001379"
     CDS             complement(12306..12992)
                     /locus_tag="ACG8FK_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5096995.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(12970..13464)
                     /locus_tag="ACG8FK_001380"
     CDS             complement(12970..13464)
                     /locus_tag="ACG8FK_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5096996.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(13484..14704)
                     /locus_tag="ACG8FK_001381"
     CDS             complement(13484..14704)
                     /locus_tag="ACG8FK_001381"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5096997.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            14851..16686
                     /gene="lepA"
                     /locus_tag="ACG8FK_001382"
     CDS             14851..16686
                     /gene="lepA"
                     /locus_tag="ACG8FK_001382"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS5096998.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(16780..17907)
                     /locus_tag="ACG8FK_001383"
     CDS             complement(16780..17907)
                     /locus_tag="ACG8FK_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5096999.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         5906 a         3238 c         3756 g         5020 t
ORIGIN      
        1 cgagatgtga cttgaagcag atgagaaggt gttcatctgc ttcaagtttt ttgtgctaga
       61 tgactaaatc agcttttgtg taagttattt ctcagtaatc aaggtgatca gtattggaaa
      121 attgaaattt agtaaggaca ggggacacaa ttacgcatga aagtagaaaa aattgttctt
      181 atatccgtgt tttttgggta ggacagtcgc gtttgtgatt tgtttcgtgt tataatacaa
      241 ctagttgtaa agaaagacac atcctttata taaaatgcga tgcaagaggt gagacaaaat
      301 gagatgtccc agatgtcaac ataataattc acgagtaatc gacagccgtc aagcagatga
      361 tggacgtgcg atccgtcgcc gtcgcgaatg tgagaattgt tcgttccgat tcactacatt
      421 tgaacgaatt gaagctgctc cgttattagt aatcaagaaa aacggggaac gcgaagagtt
      481 caatcgcgat aaaattttaa gaggactcat ccgttcagca gaaaaaagac ctgtagctat
      541 ggaacaaatg gaacaaatcg tcaaccatgt cgaaaaccgc gtacgcagtc tcggagaaaa
      601 cgaagtatcg acgaatctaa ttggtgaata tgtgatggaa gatttagttg atttagacga
      661 aatcgcttat attcgttttg caagtgttta tcgccagttc aaagatatgt ctgttttctt
      721 aaaagagttg caagatatcg tggataaagc aaaagaaaat aataaagaga ataataaaga
      781 aaacaattaa gaaacaaaaa ggaggcattt ccttggaaaa tgcttggaaa gagcttcaac
      841 caaaaaatat ttaccaagga agaaaagcgg ctccgctgag agaagacagc aagaacgcct
      901 tgctgtatct ttatcagcca ctaatcggcg gagaagcctt gagtatctat ttcaccctgt
      961 tatcagaaat ctctcaagaa tctggcaaag gtcctgaagg gatgcatgca gatctgctta
     1021 gtagtctagg ctgcggtatc ccgcaatttt atgatgcaag aaaaaagcta gaaggaatcg
     1081 gcttactgga tgtttatttt aaagaagata gtgatctggg acctcgtttt atttatgagc
     1141 tgttagaacc actcattcct aaagcatttt tcaaagactc tgttctggct tttcttcttc
     1201 tggaaaaagt tggtgaacga cgattccaga agcttaccca attctttgaa cctaaaaggc
     1261 atacagcaga gggatatcaa aaaatcacaa aaaaattttt agatgtttat cgcttcaatg
     1321 aagcagtgta cgctgctaat caagaaaaca tcgaatcgct gaataaatct tttgaagaat
     1381 ctgaatcagt agcattactt gaagcagatt cgacattcga ttggcagttt ttgaatgatt
     1441 ggctggcaaa aaaacatatt gcaagtcttg atgatccaat catcaaacaa gtcgacatgt
     1501 atcatcagct ttacggtttc gatgaattga cacttggcga actgatcagt caatcttatg
     1561 actttagtga acaaaaagta tctgaaaaag aactgcaaaa agccattttg gcaatgcaga
     1621 gacgtccggt gaagcaaaca aaagaaacag ttgcggtttc tgaaaacagt aatgaaccct
     1681 cgtcaaacga acatttgaca aacgcagcgc ttcagctgat aaaagaagca cagagcattc
     1741 caccgatgaa atatctggaa gcaatcaagt cagaaaaagg cggatatgtt tctaaacaag
     1801 aaaactggtt actgcaagat cttgtttctc aatcaggtct ttccagtagc gtgatcaatg
     1861 ttttgctgaa ttatgtacta gtgatcaaga ataatgctag cttaaatgcc agttatgtaa
     1921 atacagtggc aaatgaatgg gcacaaaaga aaatcgccac agcagaagaa gcgatccagc
     1981 acattcggca aataagtaat caagcaaaac aatcaaaaca aaagaagcaa acaaattatc
     2041 aaactggcaa gcgaaatgtt cgacgggaaa agcttcctga ttgggtcaac cagccaaaag
     2101 atgaaaaaca gatcagcagc gagaaaaaag cagagattga ccgccgtttt aaagaatacc
     2161 tggcaaaaaa ggagggtgat atctagtgga agacgtaggc aaagaattga agaggatcat
     2221 cagcaaacgc aattatcaag aaaagtatgc tgaaatgatc gctgaagtct taaatgatgc
     2281 ggatgttcaa gcttttctaa gcgcacacca agaagaattg acacaagcgg atattgaaaa
     2341 aagttacgct aaattgtatg aattcgtgca agaaaagcgt aaatatcaaa aaaacgatcc
     2401 aacgatgatc gctcccggct atgagccgaa gctgacgttg aattttcatt atgtggatgt
     2461 cacttatgta ccaacggaag cattgttagc acggcaaaga caagaagaaa tcagaagccg
     2521 agtaaaagca atggatatgc caaaagatat tcaatcggct tcttttgaaa actttgaacg
     2581 tacagatgga cgaggatatg cgagcttaga agccctagat tttgtagaga aatatactgc
     2641 tgatccaaaa gggtatcata aaggactgta tctagtagga agttttggta ttggaaaaac
     2701 atatttatta ggagcaacag cgagagaatt agcgattgct ggattcacca cgacgctagt
     2761 ccattttccg acatttgccg ttgagatgaa acaggcaatc ggaaaagatc aagtggcaga
     2821 aaagttagat gcagtgaaac gttcacctat tttgatgatc gatgacattg gtgcagatgc
     2881 gatgagtagt tggatacgag acgaagtatt cggtgttatc cttcaatatc ggatgcagga
     2941 acagttacca accttcttct catcgaattt tacaatggat gaattggaac agcatttgtc
     3001 cgtcactcaa cgtggagatg aagagccgct aaaagcgaaa cggataatgg aacgcatacg
     3061 ttatttgaca aaagaaatcg aaatgactgg tcgcaaccga cgcaatggtt gaacagttga
     3121 caagtccaac tgacatttga cctagtagta aaagcaggtc agcgaatcat tatatggaca
     3181 gaaatgaccg gagacaatca ccgcaacaag taatgcagaa acttaaacgg aggatttttt
     3241 atgagggcta ttttttctag tatatctgta gttggtctat tttttctttt agcaggctgc
     3301 ggtagcgcag atagcaacac aggttctgtt gattcgattg atgcgggtca aacagtaaaa
     3361 acaagcagta cgaaaacgga gagacaaagc atatttgtcg gaacgattga aagtatcact
     3421 caagtagaag cagaagaaga aacgcttcag ttgaaactcg tcgatgtcat ggccaaagaa
     3481 gatccagcga atatgggaac gtcattcagc aatgacggag tgatcttaaa tgctactgct
     3541 gaaaaaataa gcgtggacat aaatgaattg aaaatcggtg acaaagtcca atttacatta
     3601 gttgagatgc cgatcatgac gatgtctatc ccaccgcaag ttcctggaga ttcgattatt
     3661 gatgtttcat tgaacaaata accaataaca ttttttcaaa aataagaaaa tgttgtttta
     3721 tagaacgaaa aggtatattt tttaaggaga tatgggaatt tttttattcc tagtctcctt
     3781 attttatttg gaaaatgcta taattatcta gaaggagtga agctatgaac catttagatt
     3841 tatctcagcg cttagccgca gtgggtgact ttgtccctga atccgctcgt ttagcagaca
     3901 ttggatcaga tcacgcttac ttacccgttg cattgatatt aagaggaaaa atcgaatatg
     3961 cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc acgtaagcaa gtcaaaaaag
     4021 atggattaga agaccgtatc accgtacgcc ttgcaaatgg tctagatgct atccagccac
     4081 aagatggtat tacagctgtc acgattgcgg gtatgggagg ttcactgatc cgagatatcc
     4141 tagaagctgg caagttgaat caacgcttaa gcggacaaga acgtttgatc cttcagccaa
     4201 acatcggaga gcgtacatta cgatcttggc tgcaagaaaa tcagtataaa atccaatcag
     4261 aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat tgtagccgaa aaagaacaaa
     4321 aaccagttac ttacaatgaa atggagctga tgttcgggcc atttctgctt gaagagaaaa
     4381 gtgagatctt caaatcaaaa tggacaagag agcttcaaca aagaaaagct atccgtgaac
     4441 agctcaaaca agcagctaat caagaggcac gtcaaaaaca aatcgagcag gaaatccaaa
     4501 tgattgagga ggttttaaag ccatgacaat cagcggacgc actttcatcc aaaagtttga
     4561 aagctattgt cctcagtggc tagctgaaga aggagaccca gtcggactgc atatcggaac
     4621 attggataag ccaatccaac gagtaatgat gacattagac gttcgtccag aagtggtcga
     4681 agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa catcctccaa tctttcgtcc
     4741 tgtaaaacgg ctggttactg atcagccaca agaaaaaatg tatgcagatt tgttgaaaca
     4801 tgatattgcc gtctatgcag cacacacgaa catggatatt atttgggatg gactgaatga
     4861 ctggttttgt gaacttctcg gtattgaagt ggagagttat ttagtaaaga ctcatgagat
     4921 tcattataaa aaactggccg tttatgtacc tgtggatcat gcacagaaaa tgcgtgaagt
     4981 attagctgca gctggtgctg gtacacaagg tgattatacc ggtacaagtt ttacctcgat
     5041 tggacacggt cgatttactc cggaagctgg tgcacaacca gcgattggga aagtagggaa
     5101 gacagaacaa gttcaagagg caaaagttga ggttattttg ccggaaacta tcgaaaaaca
     5161 agtgattcaa gccatgcggt cagctcatcc ttatgaggaa cctgcttatg atcttttcgc
     5221 tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta ggtgaattgc cgcaagaaat
     5281 ttctatagag gcatttgtag aacaagtaaa agaagcgttc caactagatg gactgagaat
     5341 agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt gcgatttgcg gtggaagcgg
     5401 tgaaaaattt tatccgcaag caatcgccca aagagcagat gtctatatca cgggagatat
     5461 ctattatcat acggctcatg atatgcaaag cgctgggttg attgcaattg atcccggtca
     5521 ttatattgaa tcgttatgca agcaacgatt tatcgagaaa tttgaatctt ggaaacaaga
     5581 agaaaattgg gatattaact ttttcgtttc tgaaacaaat acaaacccat ttcaatttaa
     5641 ttaaaaggag agaatactat gtacgaaaat cttttgccac gttttttacg atatgtcaaa
     5701 acagaaacac gctccgatgc aacaagcaaa acgactccat cgactcagac acaagttgct
     5761 tttgcgcaag aattgaaaaa agaattagaa gagttaggct tagctgatgt tttctataat
     5821 gaagataacg gattcgttat gggaacatta ccaagcaatg ttgatcatga agtgcgttcc
     5881 attggattta tttcccacat ggatacagca gattttaatg cagtaggagt caatccacaa
     5941 atcattgaaa actatgatgg cgagtctacg atcgtactag atgcggaaga acgctataca
     6001 ttgaatacaa aagattttcc aaatttgaaa aattatgaag gccacacatt gatcacaaca
     6061 gatggcagta cattactggg agcagatgac aaatcaggga ttgcggaaat catgactgca
     6121 atggagatct tgatcaataa tccttctatc cctcacggta ccatcaaagt ggcttttggt
     6181 cctgatgaag aaatcggtgt cggagcggat aaattcgatg ttgagcaatt cgatgtagat
     6241 tttgcttata cagtcgatgg tggtccttta ggtgagttgc agtatgaaac gtttagtgct
     6301 gctcaagcaa atattacgat ccaagggaaa aatgtacatc cagggacggc aaaagataca
     6361 atgatcaatg ctctacagtt agcaatcgat tttcataatc agcttccagc cgatgaagtt
     6421 ccagaaaaaa cggaaggata cgaaggtttc ttccatttga tggcattgga tgggaatcct
     6481 gaagaagctt ctatgagtta tatcattcgc gatcatgatc gtgaaaaatt cgaagcacgc
     6541 aaagcacaaa tcacacatat ccaagaagag ttgaatcagc gatttgatca agaacgaatc
     6601 aaagtagata tgtatgatca atattacaac atgaaagaaa tcattgaaaa agatatgagc
     6661 atcgtggaat tagcgaaaca agcaatgatc gagcttggta tctctccagt gatcgaacca
     6721 gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg gtattccgac gccaaatcta
     6781 tttgccggag gagaaaatat gcacggacga ttcgaatttg tttctttaca agtaatggaa
     6841 aaagcaacag acgtgattgt aaaaattgca gaattgaaca caaagagtta aaatcgaaaa
     6901 agaacagctt atgacaaggc gttcgtccca atgatggaat gtcatctgtc taaggctaaa
     6961 gggcaggaca atggaattgg ttgtcccgcc ctcttttttg aagagtaaat aggaggtaaa
     7021 gaatggaaaa gctgctagtt ttttataaca aaagttcagg gaaagatgag ggtgagaaat
     7081 tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc agaattatat gtagaactag
     7141 ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa gaaagcagaa gccatagaag
     7201 ctgatacttt agtaattata ggtggagacg gcacgattca tcatatcgtc caagcgttcc
     7261 aaaaaacatt atctcaatac catgtcggat tacttccagg tggaactgtc aacaaccttg
     7321 cacgtgttct tgagattccc ctcaaaaaag aagaagctgc tgacgtgatc ctggaaggca
     7381 atgtacggaa aatagactat ggtcaagtca atgatgaagt cattatctct acactgacaa
     7441 tcggaatctt agcagataca gcagtttgga tcagtcaaga agaaaaacag cgttacggct
     7501 cgtggatctt tatcaagaag ttttttaagt tattgctgaa gaagaaaaaa tatcatttgg
     7561 atttagaaac agacgaggag cattggaaag gaaaatccca actgttgaca gtcacgatgt
     7621 ctaactccgt gggcggtttt acaaattttg acgactcagc gaccctagat gatggaaaat
     7681 ttcatgtaac gatcgtacct tccttagata ttttccgatt tattttttat ttgcctagaa
     7741 tcatgagagg caaattctat acgatccccg gcattactta tttcacagcc aaacgcatga
     7801 aaatccaagc gcaggaaaaa tctgtgggca ctcgaacgga tggagacacg acagatgatc
     7861 ttccagttga attatctgtt gtatcaaaag gattaacagt ttattcacca aagaaagaag
     7921 aaaaagctta gaaatagtag ttaacagaaa gttggaagaa gagtaatgga tgtattgtta
     7981 cacaatcctt tgtggtttta ccttttcatt gtgaattttt acttattttg tctgatgggg
     8041 tatgataaac atcaggctag aactagcggc tggcgtgttc ctgagaataa tttgatattt
     8101 gtcagcctgt taggcggtgg tttgggagga ttgatctctc gaaaagtttt ccatcataaa
     8161 actcggaaaa agaagtttac tataggattc gctgcaggtg tacttgttga tttgattctc
     8221 atttattttt tccattaagg agcaggagat gtcgtctaaa ggaacgaaaa ctaaaatcat
     8281 tctgaatctg cttttgctga ttgtgatttt tgccattatt tattatttaa tcaatcaatc
     8341 atttgcagat atctttgccg aattgctgtc taccagtgtt gttgttttgg cagcggctgt
     8401 tatattaggg acagtttacc aaatcgctga agggcgctca atcaaagaaa ttgctgaacc
     8461 tttcgcgccg aattttacaa cgaaagatgg gttcttgacc tctagctata ttgcgtttta
     8521 ccgaatcgtt tcgtttggta caggaacgct gatctcggag atttattttt ataacaaaaa
     8581 aggaatgaag ctatcccagg gagcaggggt gacagcactc catatgatta tgtacaaact
     8641 ggcagtgatc acttatgcat tagctggctt ggtcatccaa ttctctcttt tttattctaa
     8701 tggtccatca atgatctggt ttatcatcgc tggtatcctt ttgacgatcg tgattgttgc
     8761 ctttttattg gcgatgtccg tcagcttgaa tgttcaaata ttttttgtca gcttatcgaa
     8821 taagttattt aaaagtgagc gtttgcggaa atgggtggat acatgcaatc agcagatcta
     8881 ctctttaagg gaaacggtcc aaaccattat ccaagacagg actgcgctat tgcggattta
     8941 tctatggaat atgttaaaac ttgtattttg gtatgtactt ccttatttga ttctgattga
     9001 aaaccatcct aaaatagatt ttctactgac catttcgttt atcagctttg cagtagtgct
     9061 ttcaggggtt attcctacgc ctgctggtat tggttccttt gaatttgtct atttgttatt
     9121 attcagacca gttgttggaa cagttgacgc agtttcttcg cttttactgt atcgtttcag
     9181 ttcctttatc ttgccatttc tctatggatt tgtgtatgtg attgctgaac ggcgaagagt
     9241 gatcaaagaa gaaataatag aagtaaaaaa agagaataaa gaaaatcaag ggtaggtctt
     9301 ttataagatc tacccttttt atcaaatcaa agaaaaacgt tatggataag ataattttac
     9361 gtttgtttgg ctcttttttt atatacatac aatcaatttt gtttttttaa gatttatata
     9421 agtcgagaaa aaaagtcaaa aaactatgtg tatattttga ccaaaattga ccaaagcgat
     9481 ataatagttt ttgtaaagat caaagaaata agtgatctta cctttaatta ggagggtata
     9541 cctgtatgaa tattgagaaa atgactacga cgcttcaaga agcgattgct gaagctcaac
     9601 agattgcggt cactcgaaaa catcaagata tcgacattgc ccatgtatgg aaaatctttt
     9661 tacaatctaa tcattttggt cgaaatttct atacggatgc cggattggat gtcgaatctt
     9721 ttgaacatga aattgatcgt ctactggatg agtatccagt tgtttctggt ggaaacgtcc
     9781 aatatggaca aaacctcagc cagaatttct ttcgattatt gaatgaagca gaccaaatac
     9841 gtgaaagctt cggcgacgag tttctttcaa cggaagtcgt tattttagcg ttgatgaaat
     9901 taaaaaatta tccgttaact gtttatttga ataaaaacgg attatctgaa aaagaattgc
     9961 gtaaaaacat cgaagagatg agaggaggag aaagagtgac ctcacaaaac caagaagaac
    10021 aatacaaagc attagaaaaa tacggcgttg atttagtaca gcaagtcaaa agcggaaaaa
    10081 tggatccaat catcggtcgt gatgaagaaa tccgtgatgt catccgtatt ctttcacgaa
    10141 aaacaaaaaa taatcctgtt ctgataggag aacccggtgt agggaaaacg gcaatcgttg
    10201 aagggcttgc tcaacgtatc gtacgcaaag atgtgcctga aaatttaaaa gataaaacga
    10261 ttttttcatt agatatgggt gcattgatcg ctggagcaaa attccgtggt gaatttgaag
    10321 aaagactaaa agcagtcttg aaagaagtca aaaaaagtga tggacgtatc ctcttgttca
    10381 tcgatgaaat ccacaatatc gtaggggcag gaaaaacaga aggaagtatg gatgccggaa
    10441 atctgttgaa accaatgctt gctcgcggtg aattacattt gatcggtgcc acaactttag
    10501 atgagtaccg tcaatatatg gaaaaagaca aagcgttgga acgtcgcttt caaaaggtat
    10561 tagtcaaaga accaacagtg gaagatacga tcagtattct gcgtggatta aaagaacgtt
    10621 ttgagatcca ccatggtgtt aacatccatg acaacgcatt agtagcagca gctactttat
    10681 ctgaccgtta tattactgat cggttcttac cggacaaagc aatcgatttg atcgatgaag
    10741 ccagtgcaac gattcgggta gaaatgaact ctatgccaac ggaattagat caagttacta
    10801 gacgtttgat gcagttggaa atcgaagaag ctgcactgaa aaaagaatca gatgatgcaa
    10861 gtaaaaaacg cttgaaaaat ttgcaagaag aattagctga acttcgtgaa gaagcaaatg
    10921 cgatgaagat gcagtgggaa acagaaaaag aagaagtcaa ttctgtttca gcaaaacgtg
    10981 ctgaaatcga taaagcaaaa cacgaattag aagatgcaga aaataactat gatttagaac
    11041 gcgctgctgt attgcgtcat ggtacgatac ctcaattaga aaaagaattg aaggaattag
    11101 aagctaaagc aaaagacagc gagattaaga tggttcaaga gtccgttacc gaaaatgaga
    11161 ttgctcaagt cgttggcaga ttaacgggta tccccgtaac gaaacttgta gaaggtgaac
    11221 gagaaaaact aattaaattg aatgaaacac ttcataaacg agtgattggt caagatgaag
    11281 cagttgatgc ggtcagtgac gcggtaattc gttcacgggc tggattgcaa gacccaaatc
    11341 gtcctctagg ttctttcctt ttcctaggac caactggtgt cggaaaaacc gaattagcga
    11401 aagcgttagc tgagaacttg tttgactcag aagatcatat ggtacggatc gatatgagtg
    11461 aatatatgga aaaacatgct gtgtctcgtc tagtcggagc tcctccaggc tatgtaggtt
    11521 acgaagaagg tggtcaatta acggaagctg tcagacgaaa tccttataca atcgtcttgt
    11581 tggatgaaat cgaaaaagcg catcccgatg tcttcaatat cttgttacaa gtcctagatg
    11641 acggacgctt gactgattca aaagggcgag tagtagattt caaaaatacg gttctgatca
    11701 tgaccagcaa tatcggttca cagttactgc tggaaggcgt gactgctgat ggaacgattc
    11761 cagaagcagt ggctgaacaa gtcaacacgc tattacgtgg aaacttcaaa ccagaattct
    11821 tgaaccgaat cgacgacacg attttattta caccattgag cctagacaac gtgaaaggaa
    11881 tcgttgacaa aatggtggcg caattggcac aacgtctgga acatcaagaa atcctgctga
    11941 caatcagtga tgaagccaaa acgtggattg ctgaaaatgc ttatgaacca gcatatggtg
    12001 caagaccatt gaaacgattt atcactaagg aagtagaaac accactagct aaagaaatcg
    12061 ttgcaggaca tgttatgcct aaatcaaaag taacgattac tttacttgat ggacaacttc
    12121 atttcaaaac agaagagcta gaagaaatcg tctaatcttc cattgagact ataaaagaaa
    12181 aaaggggtct gacacaacta gtgtcagacc tccttttttc aaataaacag tgttccagaa
    12241 gtagtttctt cgaaaaataa gccgaaattt cttgaagctg cccgcttctg tcacaacctc
    12301 ttttattagg tatttttcac tttttctaat ttataacttt caaagtaacg ctcatctttt
    12361 ttactttgag accatgcaaa caatcccatt aaggcagcac ctatcgtatt tgtcagcaag
    12421 tcgcccatcg tatccatcaa tgctgctcgt ccgacaaaca gtgtgccatc agaagcagca
    12481 aaacgttgaa gattcatgcc taagaactgg tcgcataaga attcccagaa ctcccaaaac
    12541 acgccgcaaa gtccagcaaa cgcaaagccg aataagagga acaaccaagg actggttttt
    12601 gatatctcag catctttcat caagtaacca atcaatccat agccaagagc tgtcaggacc
    12661 attggactca ccgcatgtaa gattttgtcc caaaaagaga tgatactgat caaatgcatc
    12721 cctgtgccta aaaagacaga gatgaacaag aaaaaccagt aaaacaaaac gattgctgct
    12781 ggtattttaa gtttgaaaac tttcaaaatg acttgtggca tgaaaatcaa tgcaatccct
    12841 aataaagatt ccacgataaa tgtgattcct tgagaagtcg aatatttttg tgtgataaat
    12901 tcataaaaat tatagcagac aacgaataca ccaaaagcaa gtaaagctat ttgcaccttt
    12961 ttgatttctt taatcactga aagtccctcc atctaaaaat ctttttactt ctgaaagcaa
    13021 ctcaatttct gaagaaacca cttttccgtt caatttgatc aaacctattg tatataagtt
    13081 caggtagtga aattgatttt cagctgtttc ttgtagtgca gcgatttttt tcgggtgggt
    13141 tccgccttgc tgtcgagtat cagtatataa gccgatcact gggatgcctt tcgcataagc
    13201 cacaccgatt tccgatgcta ccccggcatc gatcgtcagc ccatctagta aagcaatcat
    13261 taaatcacta gccaacactt tttctgtgtc agcaagagca atcattcgac tgtctgcata
    13321 agcagttttg tcattgattg cttcattttc ttgaggaaga tagatcgaaa gctcagaaga
    13381 gagctctctg atttctttaa ctaactgttg gttatataac aaatcacttt tagcaaacat
    13441 tggtgcggca aaataaattt tcatctaaac atcatccttt ttatcaaaaa taatcgctaa
    13501 agtaagcttc catgtttgga atgatacgct ctagtgactt cagtgtttct tcatctgttt
    13561 ctaaccgttc gattcttgcc aaataagaag cacggcgata attcatcatc aagcaagtca
    13621 atgtttggat atccagtcga acggattttc ctataggttc atttaagaca cggacttgtc
    13681 cctgttcatc ccatgttaaa gcgaagacac cattattcca aggagcgaca ggatcttcaa
    13741 tgatgaaatg aaaagcatca gctgttccaa caaacggaaa ccgttgaagg aattctttta
    13801 catcaacgat ccgagccata aaatatggtt cgatctgctc tttgatctga ctatcttcaa
    13861 tcaaaaatgc taaaggctca tttttataaa tatctccatg tacccagtag atcatggaaa
    13921 aatgtgcgga aataaagttc cataagccat ttcgagcttc ttgatcaaga tagaacattt
    13981 cttttacgtg aaatatttct tcagctaccc agtagaacag aacacctctt ggttcatggt
    14041 tgctatcgta gtagacagcg gctgtgcgct catcttcatt ttcgaaacgc caatactctt
    14101 cccaatgaaa atcactgcgg aataaagcac catggttttg ttgggcaaat ttgtcataaa
    14161 cggaaaatac gtctggatga tcaactggta atcgctcaac cataccactt actgggattt
    14221 gtttaggtag ctgtgtatct ctgattttaa aggaaagttt atcagacatg atttcccatc
    14281 ctttacgccg atagtagggg atactgtaag gatacaaata agagatccat tgcttattct
    14341 tgcgcatatt atccaatgca acgataatta gatcttgcat caatccatgg ttagcatatt
    14401 ctggataagt tccaactcca gtcacaccac ccattttata acgtgcaccg tgaatattga
    14461 cttcacaagg atagatagcg atttgagaga tcagtttatt ttcgtggaac catccgaaca
    14521 ctttcgatac ttccaaaatc ggttttttgg aacggataaa agctcgttta ttctcaaacc
    14581 cgctttcctc gatgtctgct tcagtcactt ggaacacata ggaaagcaat tcattgaatt
    14641 gatcgatatg ttcttgagtg accggtttta attcaagatt ttctctaaaa tctttttgat
    14701 ccattttttc atctccattt attagcttga taacagtata gcaaaattct acaaaaaaaa
    14761 cttctatagc tgactaatta aaaactcttt aaattactgc ttttcgttgg tataatggaa
    14821 agtgctgatt atatgaaaag aggagacgct atggatataa agaaaatgaa agaacgccaa
    14881 gagcagattc gtaatttttc gatcatcgct catatcgacc atgggaaatc tacattggcc
    14941 gatcgtattt tagaaatgac gcacactgtg acgtctcgcg aaatgcaaga tcagctgtta
    15001 gactcgatgg atttggaacg tgaacgtgga atcacgatta aattaaatgc tgtagaactc
    15061 aattacacag cgaaaaatgg agaaacatat atctttcact tgattgacac cccagggcac
    15121 gtcgacttca cctatgaagt ctctcgaagc cttgctgcct gtgagggtgc cgttttagtg
    15181 gtcgatgcag cacaaggaat cgaagcacaa acattggcca atgtttattt agcgttagac
    15241 aatgatttgg agatcctgcc agtcatcaat aaaatcgacc tacccgcagc agatcccgaa
    15301 cgtgtccgtc aagaaatcga agacgtaatc ggaatcgatg ctagtgaagc tgtattagcc
    15361 agtgccaaat cagggattgg gattgaagat attctcgagc agatcgtgga atatgttcct
    15421 gcaccaagtg gagacttaga tgcaccgcta aaagctttga tctttgactc tatttatgat
    15481 agttaccgcg gtgttgttct aaatgtccgg atcacggacg gtatggtcaa acccggagac
    15541 aaaatcaaac tgatgagtaa tggcaaaaca tttgatgtta cagaggtcgg ggtcttctcg
    15601 ccaaaagcag ttgctagaga ctttttgatg gtcggtgatg tcggttatat cacagcaagt
    15661 atcaaaactg tccaagatac acgagtaggg gatacagtaa cgttagcaga caatcctgcc
    15721 gctgaagctt taccaggtta ccgaaaaatg aatccgatgg tttattgtgg tctttatcca
    15781 attgatactt ctcgttacaa cgatttgcgg gaagctttag aaaaactgca attgaatgat
    15841 gctgctttac aattcgaacc agaaacctct caagcattag gttttgggtt ccgctgtgga
    15901 ttcctcggac ttcttcatat ggatgtcgtc caagaacgct tggaacggga attcaatcta
    15961 gaattgatca cgactgcacc atccgttatt taccatgtta ataaaacgga tggtacgact
    16021 gctacagtag ataacccggc agattttcct gaacctgtaa cgattcagga tgtagaagaa
    16081 ccattcgtca aagcacagat catggtccca aatgattttg tcggtgcagt aatggaactt
    16141 tctcaacgaa aacggggaga attcattacg atggattatt tagatgatta ccgagtaaat
    16201 gtcgtctata atattccgct ttctgaaatt gtctttgact ttttcgacaa attgaaatca
    16261 agcacgaaag gctatgcctc attggattat gaaatgtctg gttatcaaaa aagcaagtta
    16321 gtgaagatgg acatcttgct gaatggagaa aaagtcgatg cattaagctt tatcgttcac
    16381 cgagactttg cctatgaacg tggaaaagca atcgttgaaa aactgaaaaa actaatccca
    16441 cgccaacaat ttgaggtgcc aatccaagca gctattggac aaaaaatcgt tgcccgttca
    16501 gacatcaaag ccttgcgtaa aaacgtcttg gctaaatgtt atggtggaga tgtttctcgt
    16561 aaacgtaaac tcttagagaa acaaaaagaa gggaagaagc ggatgaaaca aatcggctcc
    16621 gtcgaagttc cgcaagaagc cttcatggcc gttctgaaaa tggacgagga cgaaccgaag
    16681 aaatagcaag ggtttgtttg aaaacagtgt aaaaaataaa ccatgtttac atggatttgc
    16741 caccggtttg ccaccggtgg tttttttcgg tggcaaaaat caagaattct ttctaagatt
    16801 ttccatgata tcgaccgttt catttctcat cttatctgtt acatgcgagt aagtatccat
    16861 cgtaattgaa attctgctgt ggcccaatcg ttcagagatt tctttcattt ttgcgccatt
    16921 ttcgagaaga agtgtagcat gagtatgtct gagagaatgg aagttaaaag agagggagag
    16981 tgcattcgat atcctcctag tattccattt cactacactt ggcgtaacta gctcaccgtc
    17041 ttccttcgta catactgcat ttgaatcaat gtagagcttt ccatacttca ttcgattttc
    17101 taattgttgt ttcttatgtt ttttcagaat tgctagcaag gtttgtccaa taaaaatcgt
    17161 tcgattggaa ctgcttgtct ttggcgtacc atatacccat gcgccgtcat tctttaccat
    17221 ttgtttctct acagtaattg ttccatttga aaaatcgaca tcatcccacg tcagaccaca
    17281 aacttcgcca acgcgcattc ccgtataaaa tccaatattc aaaggaatat agaaaggatg
    17341 tccttcagga gtaatttcta gcatatgatc aaagtcctca agagaaatga tttttagatc
    17401 ttttttagtc gttggtcgtg cttcatattt tggtattttt acatacagca taggattttg
    17461 cttgattaat ccccaaggat aaaccgccat attcagcgca ttcttaagga cagagtgagt
    17521 aatagtcatt gttttcttcg agtaaccctt tttaaattca gcattgatga aattttgtaa
    17581 aagagcaggg gagagatccg taagtttttt ctttcctaaa taaccgttta tatgattttt
    17641 gatggtaaat cggtagtttt cataggtatt gtattttaga tttagtttaa cgtattcctc
    17701 catccaaaaa tcaaggtatt gttttactcg agtatccgta cctaaaaagt attgtcctgt
    17761 ttcgtcaata tctgataaaa cttttcgtaa agcagcttcg gcctctggtc gggtgtctcc
    17821 gccaactttc tccacttttt ttcttgagcc atcatcattg atatcttcgc cgtttgtata
    17881 attaagcgag acaaataaaa aagccattta tgttacactc
//
LOCUS       DAZUMV010000044        17762 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000044 DAZUMV010000000
VERSION     DAZUMV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.044"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..863
                     /locus_tag="ACG8FK_001384"
     CDS             51..863
                     /locus_tag="ACG8FK_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS5097000.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            860..1660
                     /locus_tag="ACG8FK_001385"
     CDS             860..1660
                     /locus_tag="ACG8FK_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS5097001.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1821..3089
                     /gene="rseP"
                     /locus_tag="ACG8FK_001386"
     CDS             1821..3089
                     /gene="rseP"
                     /locus_tag="ACG8FK_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS5097002.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3157..4866
                     /locus_tag="ACG8FK_001387"
     CDS             3157..4866
                     /locus_tag="ACG8FK_001387"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS5097003.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5078..9430
                     /locus_tag="ACG8FK_001388"
     CDS             5078..9430
                     /locus_tag="ACG8FK_001388"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS5097004.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9574..10047
                     /gene="rimP"
                     /locus_tag="ACG8FK_001389"
     CDS             9574..10047
                     /gene="rimP"
                     /locus_tag="ACG8FK_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS5097005.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10071..11246
                     /gene="nusA"
                     /locus_tag="ACG8FK_001390"
     CDS             10071..11246
                     /gene="nusA"
                     /locus_tag="ACG8FK_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS5097006.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11268..11561
                     /locus_tag="ACG8FK_001391"
     CDS             11268..11561
                     /locus_tag="ACG8FK_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS5097007.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11558..11869
                     /locus_tag="ACG8FK_001392"
     CDS             11558..11869
                     /locus_tag="ACG8FK_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS5097008.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11882..14188
                     /gene="infB"
                     /locus_tag="ACG8FK_001393"
     CDS             11882..14188
                     /gene="infB"
                     /locus_tag="ACG8FK_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS5097009.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14215..14562
                     /gene="rbfA"
                     /locus_tag="ACG8FK_001394"
     CDS             14215..14562
                     /gene="rbfA"
                     /locus_tag="ACG8FK_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS5097010.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14670..15174)
                     /locus_tag="ACG8FK_001395"
                     /pseudo
     CDS             complement(14670..15174)
                     /locus_tag="ACG8FK_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15339..17702
                     /locus_tag="ACG8FK_001396"
     CDS             15339..17702
                     /locus_tag="ACG8FK_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFS5097011.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6056 a         3150 c         3913 g         4643 t
ORIGIN      
        1 gctataatac ttaagatagt gggaatcttt taagtatagg aggaaaaaga atgttacgtt
       61 tttttccgca aaaaaacaaa tatattcaag aaaaaagcga atttcaattt aataccgatg
      121 gaccaattcc tcagcacatt gccatcatca tggacggcaa tggtcgttgg gcgcaaaacc
      181 gtcgtttgcc acgagttgct ggccataaag aaggcatgga aacagtaaag aaagtcacaa
      241 aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta tgccttttcg actgaaaact
      301 ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact acctgtcgac ttctttgata
      361 cgttcgtacc agaattgatt aaagaaaatg tcaaagttca tgtcatgggt tatgaaaacg
      421 tattgccaga acatacgcaa gatgctgtaa gacgtgcaat tgagcaaaca aaaaacaata
      481 ctggaatggt attgaatttt gctttgaatt atggaagtag agcagagatc gtgactgctg
      541 tccaagaaat tgccgaagaa gtagcaaaag gagaaataca cgcagaagaa atcgatgatg
      601 aattgattgc taagcattta atgacaggtt tcttgcctaa agagttacaa gatcctgagc
      661 tgatgatccg tacaagtgga gaagagcgaa ttagtaattt tctgttatgg cagatcgcat
      721 atagtgagct gtattttaca aaagcgcttt ggcctgattt tgatggtgcg catcttgaag
      781 aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg cggtgtaaaa aaaacagaag
      841 atgagaaagg ggatcagtca tgaaacaacg tgtgatcaca gcagctgtgg cgctagtctt
      901 gtttattccg attatatgga tcggaggcat agcggttgag cttacagcag caatcttagc
      961 ggtagtagga gtttacgagc tgttccggat gaaaggattg acactcttaa gttttgaagg
     1021 agttttatcc gccgttggtg cagtgttact tgttttgcca aaagaaagat ggtttttctt
     1081 tcttccagag aatgtaagta catttatgtt attttatctt atagttatga ttcttttagg
     1141 aacatcagtt atttctaaaa atacgtatac gattgatgaa gcaggttttc ctgtgcttgt
     1201 cagcttgtat gtgggaatag gtttccagaa ttttgtcaat gcacgaacag aaagcttggc
     1261 tgttttgata tttggtctat ttattgtttg ggcaactgat atcggagcat atatggttgg
     1321 tcgacgttat ggtcagcata agttaagtcc tgatatttcg cccaacaaaa cgattgaagg
     1381 tgcgctcggt ggtatcggta gtgctgttgt agtcgcattt ctttatttat tgctataccc
     1441 agcaaaagaa ctattcaatc atggtatgtt tgtcatgctt ctgatgacga ttattttttc
     1501 agcagtcgga caatttggtg atttggtcga atctgcaatc aaacgtcatt atgatgtgaa
     1561 agactcggga aatatcttac caggccatgg tggaatctta gaccgtttcg acagtttgct
     1621 gtttgtattt cctatcatgc atttatttgg cattatttag taagtatacc tgcgtttctt
     1681 tgtgaatttc ataaaagacg caggttttta tttttcatta tattgatact attggtgtct
     1741 ttggtatact atggtaaaat tatagtgttt tatcgttgtt ttataaaaaa cacaaaaaac
     1801 aagtaaagga gaaaaaagct atgaaaacga ttctgacatt tatcatcgtt tttggtatat
     1861 tagtgattgt tcatgagttt ggtcatttct tctttgcgaa acgatcagga atcctcgtcc
     1921 gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca tcaagcaaaa gacgggacaa
     1981 catatacgct tcgcctatta cctattggcg gttatgttcg aatggcaggc aatggggatg
     2041 atgaaacaga aatggcacca ggaatgcctc tttctctttt actaaattct gacggcatcg
     2101 tagagaagat aaatttaagt aagaaaatcc aattgacaaa tgctatcccg atggaattga
     2161 gccgttatga tttagaagat gagctgacta tcactggtta tgtgaatgga gacgaaacag
     2221 aagtagtaac ctaccctgtt gatcacgatg cgacgattat tgaaaacgac ggaacagaaa
     2281 tcagaattgc gccaaaagac gtacagttcc aatcggctaa attgtggcag cgtatgctga
     2341 ccaactttgc tggacccatg aataacttta ttttggcaat cgtgttattc atcattctgg
     2401 catttatgca aggtggtgtt caagtgacga acactaatcg cgtaggagag atcatgccta
     2461 atggagctgc ggcagaagca ggcttaaaag aaaatgatga ggtagtcagt gtggacggaa
     2521 aagaaatcca ttcatggaat gatctgacaa cagtcattac gaaaaatcca ggtaaaacat
     2581 tagactttaa gatcgaacga gaaggtcagg tgcaatctgt cgacgtgaca ccgaaatctg
     2641 tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa agccccaatg aatacaggtt
     2701 ttatggataa gatcattgga gggacgcgtc aagctttcag cggttcattg gaaattttca
     2761 aagccttagg atcactattt acaggtttca gtttagataa attaggcgga cctgtcatga
     2821 tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac aactgttatt agtttgatgg
     2881 cgcttctttc aatgaatctc ggaatcgtca atctgcttcc gattcctgcc ttagatggcg
     2941 ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa accattaagt caagaaaaag
     3001 aaggaatatt gacattagct ggctttggat ttttaatgtt gttgatggtg ctagttacat
     3061 ggaacgatat tcaacgattc tttttctaga aaaagttgac aaatagggta taataacaaa
     3121 cgataactat tttgaggaaa taaaggagta tatagaatga gacaatcaaa aatgttgatt
     3181 ccaacattac gcgaagttcc aaatgatgca gaagtattaa gccatcaaat cttgctaaga
     3241 gctggatata tccgccaagt atcagcgggt atttattcat atctgccatt agcaaatcgt
     3301 gtattggaaa aactgaaacg cattatgcgg gaagaatttg aaaaaatcgg agcggtagaa
     3361 atgctgatgc ctgccatctt gccagcagaa ctttgggaag aatccgggcg ttacgaaaca
     3421 tatggaccaa acttgtatcg tttcaaagac cgcaatgatc gaaaaatgat ccttggacca
     3481 actcacgaag aaacttttac tgatttgatg cgtaatgaaa tcaattcata taagaaatta
     3541 cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg aaaaacgacc tcgcttcggt
     3601 ttgcttcgcg gaagagagtt tatcatgaaa gatgcatatt ctttccatgc aacggaagaa
     3661 agtttggacg agacatataa agattacgaa aaagcctata ccgaagtatt caagcgttgt
     3721 ggcttagaat ttcgctcgat catcggagac ggcggcgcaa tgggcgggaa agactcaaag
     3781 gaattcatgg cgatctcaga aatcggggag gatacgatct gttattcgac tgaaagtgat
     3841 tatgctgcaa acttggagat ggctacaagc tactatgtat caaaaaaatc gcatgaaaca
     3901 cagcttgaat tggagaaagt agctacacct aacgtaaaat caattgacga agtagcagat
     3961 ttcttttctg ttgaacctca aaaaatcatc aagtctgtat tgttcattgc ggatgaacaa
     4021 cctgtccttg ttttggtacg tggagaccat gaagtcaatg acgtaaaact gaaaaacttt
     4081 ttatctgctg actttttaga agaagcaaca gaagaagaag cacaaaaata tctaggtgct
     4141 gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg taaaagtata tgcagaccgt
     4201 tatgtccaag atttagcgaa tgccattact ggagcgaacg aaacaggatt ccactataaa
     4261 aatgtgaatc caaaacgcga tttcactgtg ctttcttatg aagatcttcg ttttgtacaa
     4321 gaaggagatc cttcaccaga caacaacggt gttcttgcct ttactcgagg aatcgaaatc
     4381 ggacatatat tcaaaatcgg cactcgttac agtgaatcta tgggagcaac tgtactagac
     4441 gaaaatggac gagaaaaatt tgtgattatg ggctgttatg gtatcggtgt aagtcgttta
     4501 ttatcagcaa tcgtagaaca acattcagat gaacaaggaa tcaattggcc aaaaggaatt
     4561 gcgccatttg atctgcatat cgtccaaatg aatctaaaag acgaatacca aacaagcctg
     4621 acgaatgaac tagaagaagc gatgacaaaa gctggctatg aagtactggt tgatgacaga
     4681 aatgaacgcg caggagtgaa attcgcggat tctgatttga ttggctgtcc aatcagaatc
     4741 actgtcggta aaaaagcagt ggatggaatc gtagaagtga aaatcaagaa aactggcgaa
     4801 atggttgaag tacgtaaaga agaattggcg aatactttac caatcctctt gaaccaaggc
     4861 gaataataag cgttaaaaca cagaaaataa aaatagctga atcagctgtt tttgacaatc
     4921 tgatgattcg cttttaaaat tggaactgcg atttgaatcg cagttccaat tttttaatgt
     4981 aaaactcaga aaaaacgagt atactaaaag gtactgaaaa agaagaggat aagatgcgat
     5041 caattgcatt gtttctcatc agaaagggcg tatatctttg tcagaaaaac gagatttatt
     5101 tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc cactgcttac
     5161 tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg cgtttatggg aatttacgct
     5221 acatttttcc aaaattttgc ctattatgct atatcggtca ttgactcagc atctgactat
     5281 cgcttttaaa gatattgcac aagtcaaact gaatatctta gcagatgacc agacattcga
     5341 tgaacagctc ttgcaagatt actgggccca agctttggaa aatcagcaat gcgatacacc
     5401 actagcacag caagtattga aaacgcaggt acctgtgata aaagatcgta aagtcatctt
     5461 gccaatcgac agtacaggag ctatttctta tttgaagcaa caatacttgc cattagtaga
     5521 agagttgttc gtcagttacg gattcccaaa atttcgtatc gaaccagaag tggatgagca
     5581 gcaagcggaa cgtgtattga aactttttga agaacgcaaa caagaacagg cagaagcctt
     5641 tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg aaagaaaaga
     5701 gcaatcgcca gctcttgaag gacccatcca gctaggacga aatattccag ccgatgaacc
     5761 aacgacacca atgatcagta tcgttgaaga agaacgaaga gtcacacttg aggggtacgt
     5821 attcgacaaa gaagtcagag aactacgttc aaaacgcaag atcttgactt tgaaaatcac
     5881 agattatact tcatcattta tcgtcaaaaa attctcgaac ggcgaaaaag atgaacaagt
     5941 atttgatgcg atcagtgtcg gtagctggtt aaaggtacga ggaagtattc aagaagatac
     6001 gtttgtccgt gatttggtca tgaatgcgca agatatcata gaagtcaaac atacacctcg
     6061 caaagattat gctcctgaag gggaaaaacg agtagagctt catgttcata gcaatatgag
     6121 tacgatggat gcaacaaaca gcatctctga tcttgtggca caagcaggaa aatggggaca
     6181 ccgagctatc gccattacag accatggtgg tgcgcaagcc tttccggaag cacatagtgc
     6241 tggaaaaaaa gcaggtgtga aaatcttata cggcgtggaa gcgaatgtcg tagatgatgg
     6301 tgtcccaatt gcttataatg atgcacatga agcattgagt gaagccacat acgtcgtatt
     6361 tgacgtggaa acgacaggat tgtctgcagt atatgacacg atcatcgagc tagccgcagt
     6421 caaaatgtat aaaggaaatg tgatcgaaag ctttgatgaa tttatcgatc caggacatcc
     6481 attgtcacga acaacggtag atctgactgg tatcacggat gaaatggttc gagggtcaaa
     6541 gtcggaagaa gaagtattgc ggatgttcct tgaattttca aaagatacaa ttttggtagc
     6601 ccacaacgcg gcttttgata tggggttctt gaatactagc tacgcaaggt atggtattcc
     6661 tgaagcggcc aatccagtca ttgatacgtt ggaattagct agatatctgt atcctcaatt
     6721 caaacgtttc ggattaggtg tgttatctaa aaagtttggt gttagcttgg aacagcatca
     6781 ccgagcaatc tatgatgctg aagcaactgg tcacttagca tggatctttg tgaaagaggc
     6841 aatggacaat cataacatgc tttatcatga tcagctaaat gagcatatcg gagaaggaga
     6901 ttcttacaaa agggctcgac ctttccatgt aacgatttta gcaaaaaatc aggcaggact
     6961 gaaagatttg ttcaaactga tttcaatgtc gaatgtcgaa tacttcgaac gcgtcccacg
     7021 aataccacgc tcccaattga aaaagatgcg tgaaaatcta ttgatcggct cagcttgtga
     7081 taagggagaa atattcgaag ccatgatgca aaaaggggta gaagaggcaa gaaaccgagc
     7141 aaaattctat gattatattg aagttatgcc aaaagcggtc tatgcgcctt tgatcgagca
     7201 agaattggta aaaaatgaac atgatttgga agaaattatc caaaatttgg tagaaatcgg
     7261 aaaaagtttg gataaaatcg ttgttgcaac aggtaatgta cattatctga acgaagaaga
     7321 tgccatttac cgaaaaattt tgatcaattc tatgggcgga gcgaatccgt tgaatcgtca
     7381 tagtttgccg gatgtccatt tccgaacaac cgatgagatg ctaactgcat ttcacttttt
     7441 aggggaagaa acagcgaaag agatcgtagt agaaaatacg aataaaatcg cagatatctg
     7501 cgaagaagtg attccagtca aagatgaact ttatacaccg aagattccag gttcagaaga
     7561 tgaaatatca gaattgagtt acacaaaagc aaaacaaatg tacggagatc cgttacctga
     7621 gatcattcaa aaacgattag aaaaagagtt gaattctatt aatggtaatg gcttctcggt
     7681 tatttatctg atcgcccaaa aactagtgca taaaagtaat gaagatggct atctagtagg
     7741 ttcacgtggc tctgttggct ctagctttgt tgcgacgatg acaggaatca ctgaagttaa
     7801 tccattagcc cctcattatt attgtccaga gtgccaatat tccgagtttt ttgaagatgg
     7861 tacgtatggt tctggatttg acatgccaga aaaacaatgt ccaaaatgtg gtgcacgctt
     7921 aaacaaagac ggtcatgata ttccatttga gacattttta ggattccatg gagacaaagt
     7981 gcccgatatt gatttgaact tctcaggtga ttatcaagca gaagcccata actatacgaa
     8041 agtattgttt ggcgaagact atgtttaccg tgccggaacg attggtacag tcgccgacaa
     8101 aacagcatat ggctatgtaa aaggatacga aagggacaat aatctgcagt ttcgaagtgc
     8161 agaagtcgac agattagcaa aaggcgctac tggagtcaaa cggacaaccg gccagcatcc
     8221 aggggggatc atcgttattc ccgattatat ggatgtctat gattttacgc cgatccaata
     8281 tccagcagac gatcaaaatt cagaatggaa aacgactcat tttgatttcc actcgattca
     8341 cgataatgta ttgaaattag atattctagg gcatgatgac ccaactgtta ttcggatgct
     8401 tcaagattta tcagggatcg acccacaaac gatccctacg gatgacccgg aagtgatgcg
     8461 gatatttgcc ggtcctgaag tacttggagt aagtcaggaa caaatttatt ccaaaacagg
     8521 gacattaggt attccagaat tcggcactcg ttttgttcga gggatgttag aagaaacgca
     8581 tcctacaact tttgcagaac tactgcaaat ttccggacta tctcatggta cagacgtatg
     8641 gttaggaaat gcggaagaat tgattcgacg aggagatgcg acactggcgg aagtaatcgg
     8701 ctgtcgtgat gatattatgg tttatttgat ccatgctgga ttagatagcg ggatggcatt
     8761 taaaatcatg gaaactgttc gtaaaggaca atggaacaag atccctgatg aattgcgaga
     8821 aacctatcta tcggcgatga aagaaaataa tgtgcccgac tggtatatcg attcttgttc
     8881 aaaaatcaaa tatatgtttc cgaaagccca tgctgcagct tacgtattga tggctcttcg
     8941 tgtcgcttat ttcaaggttt atttccctat tttgtattac tgtgcatact tttctgtacg
     9001 tgcagacgac tttgatctag tctccatgtg caaaggaaaa gatgctgtga aacaagcaat
     9061 gaaagaaatc acggacaagg gattagatgc ttccgtaaaa gaaaagaatc aactgacagt
     9121 gctggagtta gccaatgaaa tgctggaacg aggattcaaa tttggaatga ttgatttgta
     9181 taaatcggat gccgttaact ttgttattga aggtgatacg ttgatcgcac catttagagc
     9241 tgtcccaagt ttgggaacca acgtggcgaa acaaatcgtt gaagcaagaa aagacggtcc
     9301 atttttatcc aaagaagatc ttgccacccg cggtaaagtt tcaaaaacat tgatcgaata
     9361 tatgaacgac aatggcgtat tgaaagatct accagatgaa aatcaattat cgttattcga
     9421 tatgctataa gcatctgttt tcctgtctgc ttgtaatttt aagcagagta tggtatagtt
     9481 cttataggta atgatactaa ctgtgagtga gcggattttt cgctcactct ttttaatgga
     9541 attacgcact taatttttaa ggaggcgaaa cttttgagta gcgtcgttga aacagtcaca
     9601 gaaatggtga caccgatttt agatgaacaa aattttgaac ttgtcgaagt cgaatttgta
     9661 aaagaaggaa aaagctggtt cttacgagta tttattgaca aagaaggcgg tatcgatatc
     9721 gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac ttgatgcagc agatccagat
     9781 ccgattcctc aagcttattt cctggaggta tcctccccgg gagcagagcg gccattgaaa
     9841 aaagaaagcg actatgaaaa ggcacttggt gattatgtcc atgtctcctt ataccaacca
     9901 gtagatggcg aaaaacaata tgaaggattt cttcaatcat ttgatgctga acagcttaca
     9961 ttgaaaatac gcataaaaac acgagaaaaa gaattgactt ttgaccgaaa aaatattgca
    10021 aaagctcgct tagctatcca attttaatca cggagaggaa tcagaaaaaa atgagtaaag
    10081 aaatgttaaa tgcgttagac gctttagaag ctgaaaaagg gatctcaaaa gaaatcgtga
    10141 tcgatgcttt agaagcagca ttggtttctg catacaaacg tcactatggc caagcacaaa
    10201 acgtcgaagt ggaatttgat cagaaaaaag gaaagatcca tgtctatgcg gtaaaagaag
    10261 taacggaaga agtgatggat tcacagttgg aagtctcatt gaaagatgca cttttaatca
    10321 atccagcata tgagatcggt gatacgatcc gttttgaagt aacaccaaaa gattttggac
    10381 ggatcgctgc gcaaacggct aaacaagtta ttttacagcg tgtacgtgaa gcagaaagaa
    10441 cgattattta taacgaattc agtgcttatg aaaaagacat catgcaagga atcgtcgaac
    10501 gccaggataa acgttatatt tatgtcaatc ttggtaaaat tgaggcagtt ctttctaaac
    10561 aagatcaaat gccaaatgaa ttttatcaac cacatgatcg aatcaaagtt tatgtttccc
    10621 gagtagaaaa tacgtctaaa ggtccgcaag tatttgttag ccgcagccat ccagacctat
    10681 tgcgtcgctt atttgaacaa gaagtaccag aagtctatga tggaatcgtt gaaattgtca
    10741 gcatcgcccg tgaagctggc gatcgttcaa aagtggcagt tcgctcaaat gatccaaata
    10801 tcgatgcagt cggtacatgt gtcgggccaa agggacaacg agttcaagca atcgtcaatg
    10861 aattaaaagg cgaaaacatg gatatcgtcg aatggaatga agatccagct gtattcatcg
    10921 caaatgcatt gaatcctgca caagtcatgg atgtgatctt cgatgagaca aatccaaagg
    10981 cctgcacagt tgtcgttcca gactaccaat tatcgctagc aatcggtaaa cgcggacaaa
    11041 atgcacgttt agctgctaaa ttgactaacc ataaaatcga tatcaaatca gaatctgaca
    11101 tgacggaatt ttatgaaaat caagaacaac aaaaagaaac agaagaactg catgatgaag
    11161 caatcgttca gtcagattta acagacgatg aatatgaaac aattgcattc aacaatgaaa
    11221 cggtcgaaga aaaaccagaa gcttaacttt agtaaggagg caaagagatg aaacaaagga
    11281 aaattccttt acgcaaatcc gtggtttcag gtgaaatgaa acctaaaaaa gagatggtcc
    11341 gaatcacccg ttcaaaagaa ggggaagtat cgatcgatcc aactggaaaa atgcctggac
    11401 gaggagcgta tgtctcactt gagccggaag aagtacaaca agcttgggat aaaaaaattc
    11461 tcgatcgagt actcgaaaca acattaacag atgagtttta tcaagaactt cttgactatg
    11521 tcactcacca aaaagcacgt aaagagttat ttggaaaatg aatacagtta atcggcaaaa
    11581 agccatgaat ctcatcggtc tagcgatgcg cgcgggaaaa ctaatcacgg gtgaagaact
    11641 gaccattgct gaaatccgca aacaaaaagc aaaattggtg tttgtggcaa ccgatgcaag
    11701 tgagaataca agaaaaaaga taaaagataa gagttcatat tatgaagttc cttgctttga
    11761 gctattttct gaagcagaaa tcacgcagat gatcggaaaa cctcgcaagg tattcggaat
    11821 aatggatgcc ggatttgcga agaagaccaa ggagctaatt gaaggttagg aaggtgattg
    11881 catgggcaac aaaagaatct atgaattagc aaaagaactc aaaaaatcta gtaaagacgt
    11941 cgtggaaaaa gcacaacaac ttggcatcga tgtgaaaaac catatgggtg cgattactag
    12001 cagtgatgag aagaaattgc aacaagcgtt caaaaaccca agcacgaaac aacaagctgc
    12061 acaacaagca agcagaaaac cagccaatca acagccgaac gatcaacgta aaaaagaaac
    12121 aacgaacaat caacaaaaaa ataaaaataa ccgcagttat caagatcgcg gtcaagggag
    12181 cggtcaagtg aatcaaggaa aaaaaccaac aacaaaccaa aataatacgg gaaataccag
    12241 cagccaaaat cgtcaaggaa cgagccaagg cggcaatcaa aaccgtacta acaataacaa
    12301 caaaccaaac aataacaatc gcggcaaatt taataataat aaccgtaatc gttataataa
    12361 aaaaggtaaa aaaggcaaac agcaacaatc aactaaacca gctgtaccac cacgcaaatt
    12421 ccgtgaattg ccagatgtat tagagtatac tgaaggtatg aacgtagcag atattgcgaa
    12481 aaaaattcat cgtgagccag cagaaatcat taaaaaatta tttatgcttg gtgtcatggt
    12541 caatcaaaac caagcactgg ataaagatac gattgaactg ttagcgacgg actatggcat
    12601 ggaaccacaa gaaaaagtcc aagttgacat tgctgatatc gataaattct tcgaggcaga
    12661 agaagtcgat ccagataaat tagtctcacg tccgccagta gtaacgatta tgggacacgt
    12721 agaccacggg aaaacaacct tattggatac attgcgccat tcacgtgtaa caagcggcga
    12781 agccggcgga atcacacagc atatcggtgc ctaccagatc gatattgatg gtaaaccaat
    12841 cactttcttg gatacaccag gacatgcggc ctttacaagt atgcgtgcac gtggagcaag
    12901 tatcacagat atcacgatct tagttgttgc tgcagatgat ggcgtcatgc cacaaacagt
    12961 tgaagcaatc aaccatgcca aagctgcagg cgttccaatc atcgtcgctg taaataaaat
    13021 tgataaacca ggtgcaaatc ctcagcatgt tatgcaagaa ttaagtgagt atgaattaat
    13081 tcctgaagca tggggtggag aaacgatttt tgttgagatc tcagcgaaat tcggccaaaa
    13141 catcgaagaa ttgctggaaa tgatcctgtt agtcgcagaa gtagaagatt taaaagcaga
    13201 tccaacacaa cgcgcaatcg gaacagtgat cgaagcacgt cttgataaag gtaaaggtcc
    13261 tgtgtcaacc ttacttgtcc aagaaggaac acttcacgtt ggtgacccaa tcgttgtcgg
    13321 taatacttac ggacgtgtcc gtgtaatgac aaacgatctt ggccgtcgcg ataaagaagc
    13381 aggacctgca acgccagtag aaatcactgg cttgaatgat gtacctcaag caggtgaccg
    13441 tttcgtagtc ttcgaagacg aaaagacagc acgtgcagcg ggtgaagaac gcgcaaaacg
    13501 tgcattattg gaaaaccgtg ctgccacaag ccgtgtgact ttggacaact tatttgaaag
    13561 cctgaaagaa ggcgaattga aagaagttaa cgtaatcatc aaagcagatg tacaaggttc
    13621 agcagaagca ctggctgcat cattgaagaa gattgatgtg gaaggtgtcc gtgtaaaaat
    13681 cgtccattca gctgtcggag caatcaatga aagtgatgta accttagcag cagccagcaa
    13741 cgctattatc atcggtttca acgttcgtcc tacaccgcag gcaaaagtgc aagctgatac
    13801 tgaagaagtc gatattcgtc ttcaccgtat catctataaa gccatcgaag aaatcgaaac
    13861 tgcgatgaaa gggatgctag accctgaatt tgaagaaaaa atcaccggtc aaatgatcgt
    13921 tcgtgaaacc ttcaaagttt ctaaagttgg tacgattgca ggtgcctatg taacagaagg
    13981 atatattcgt agagatagcg gtgtccgtgt aatccgtgac ggaatcgtaa tctatgaagg
    14041 ccaacttgca agtctgaaac gattcaaaga tgacgtgaaa gaagtcaaaa tgggttatga
    14101 atgtggtgcg atgattgaaa aattcaatga catcaaagta gatgacgtaa ttgaaggatt
    14161 tatcatggaa gaagtcaaaa atgactaaaa actgataaaa ggaggccaat cattatggct
    14221 aactaccgtg atcgccgagt cggtcaagaa atcttaaaag aagtcaatga tattttgcgc
    14281 aaaaaggtcc gtgaccctcg tgtggagaac gtaacgatca cagatgtgaa agttacgggg
    14341 gacttacagc aagcaacgat ctattacagc attttatctg acttagcatc agataagaaa
    14401 aaagcacaag atggattgaa taaagcatca ggattgattc gtcgagagtt agggcacaat
    14461 ctaagtattt ataagacacc tgaattgttc tttgaacttg atgaatcagt tgtctatggg
    14521 aaccatatcg atgaattgct tcgcgacttg aacaaagact aatcaaaaaa tcccgcaaat
    14581 cctgtgaaat caggggttgc gggatttttt tgttttcatt aagacgtatt acctaagagt
    14641 tccatgactt tagtctggta gcgtgtcagc taaccaaaaa agagaaaaag aaaagtaaaa
    14701 aattcgactt tcttttttct atagtatata aatttttgca tatagataaa gatcaagata
    14761 ccgataacgg aagctagatt gtaatctatt ttagtgacag tcaggtatag ccaagtaact
    14821 agaagatcag tggaacctgc accataaaag tttgagtttg tcggtccgtt gacagttaaa
    14881 agaaaaatca cgttgaagtt attgatattt ccgattacgc cgcttcgatt tggtcacgat
    14941 ccaaattttg gataatccct gtagaaatta gcatagtagc tgggatacca attcacatat
    15001 tgacaaagat aacagagact tttacataaa acggatcaat caggaaagga attgctcgat
    15061 ccagccccag ttttccaaca atgtgttaac tggcctagcg tcattcagca aattatgcat
    15121 gatgaggagc gaaatgaact gcggaacagc cattgtgata acgacaagtt ttttggatca
    15181 atgtaaaaaa cgtcagcgaa aggaaaaatt tcgtggtttt ttatacctgt tcatttggct
    15241 agatataaaa tccaaacaaa tgaacagaag tgcttattag gtttcaattg ggagtatctg
    15301 cgctagaata agagtaataa tgaaaaggag tgttatgtat ggattattct agtaaagaat
    15361 actttgataa aatgacggct tggtggcgtg ctgctaatta tctttctgtt gggcaacttt
    15421 atcttaaaga taacccctta ctgagacgta cactgaaacc agaagatgtc aaaaaacatc
    15481 caatcggtca ttggggaacg attcctggcc aaaattttat ttatgtccat ttgaaccgag
    15541 tcatcaataa atatgatttg aacatgtttt atatcgaagg gccaggacac ggcggacaag
    15601 tcatggtatc gaatgcttat ttagatggaa gttacacaga gatctatcca gaagtaacgg
    15661 aagatgagac aggaatgcaa aaattattca agcgattttc atttcctgga ggcattgctt
    15721 ctcatgcagc accagaaaca ccaggttcta tccatgaagg cggagagctt ggatactctc
    15781 tttcacatgc agtcggagct gtattagaca atcctgaagt aatctctgct gtcgtgattg
    15841 gtgatggtga agcagaaacc ggtcctttag ctggaagctg gttctcaaat gtctttatca
    15901 atccagtgat agatggagct gttttaccga tccttcattt aaatggtgca aaaatagcca
    15961 acccaacgat cttggcacgt aaatcagatg gagaattagc aaattacttt aacggacttg
    16021 gctgggagcc tttctttatt gaaggaaatg atccagaaaa attaaatcct gttatggctg
    16081 aaaaaatgga tcaagcaatc gagaaaatca aatccatcca aaaagaagct cgcctgaaga
    16141 cagcaacaga tgtcgtgatg ccaaaatggc cagtattgat cgtacgcaca ccaaaaggat
    16201 ggaccggtcc aaaagaatgg gacggggaac caattgaagg aaccttccga gcacaccaag
    16261 taccgattcc tgtagatcaa gaacatatgg atcatgcaga tgctttactc cgttggctga
    16321 aatcctatga accagaaaaa ttatttgatg cacaaggacg tattttagaa gaaatcagag
    16381 aaattgcgcc tactggtgac caaagaatgg caaaaaatcc tatcaccaat ggtggaatcg
    16441 atccaagacc gcttattatg ccagattgga aaaaatatac tttgcaattt gaaaaaccag
    16501 gctccgttaa agcagaagat atgaccgaac ttggtaaatt cgttcgcgag atcattgaga
    16561 aaaatccaga aaatttccgt atatttggac cagatgaaac aaaatcaaac cgattgaatc
    16621 aagttttcaa aacgacaaat cgccaatgga tggaaaaaat cgaaccagaa aatgatgaat
    16681 ggctttcgcc atctggtcgc gtcatcgaca gtcaattatc ggaacaccaa gatgaagggt
    16741 tcttagaagg gtacgttctg actggacggc atggtttttt tgcaagttac gaatcattct
    16801 tgcgagtggt tgactctatg ttgactcagc acttcaaatg gatacggaaa tcacatgacc
    16861 tttcttggcg aaaagattat ccttcgctga atctcattgc aagctcaaca gtcttccagc
    16921 aagatcacaa tggatatagc caccaagatc caggaatatt gacacatttg gcagaaaaga
    16981 aggcggagtt catccgtgaa tatctgcctg cggatgcaaa tacattgctc gcagtcatgg
    17041 ataaagcatt ccgctcgtca gaaaaaatca atttgatcat ctcttccaaa catccgcgtg
    17101 ctcaatttta ctcagcagaa gaagcagccg tcttagtgaa tgaaggcttg aaaatcattg
    17161 attgggcttc gacagcaaaa gaggaggaac ctgaattggt cattgctgct gcaggaacgg
    17221 aatctaactt agaagcatta gcagctgtaa ctttgctgct ggaagaattc ccaaaactaa
    17281 aaattcggtt catcaatgtc gtagatttat tgaaattacg tcatccaagt caagatccgc
    17341 gtgggttgag cgatgaagaa tttgatcaat attttacaaa agacaaaccg atcttatttg
    17401 ctttccacgg ttatgaaaca ttggtccgca cgattttctt tgatcgtcat aaccatcatt
    17461 taatgattca tggctacaaa gaaaatggag atatcacgac accatttgac atgcgtgtag
    17521 taaatgaact tgatcgttat catttggcaa aagacgcagc tttaaaaatc aaaggaagtc
    17581 aagcagaaga ttttgcggaa aaaatggatc agaaattaca agaacatcaa aactatattc
    17641 gtgaaaatgg tattgatctg ccagaagtat tggattggaa atggaaaaat cttgaccaat
    17701 aaaatgataa aatcgatccc gcaaaacaga attttttaca ttgtattatt tgtcaaatta
    17761 ag
//
LOCUS       DAZUMV010000045        16724 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000045 DAZUMV010000000
VERSION     DAZUMV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.045"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..832)
                     /locus_tag="ACG8FK_001397"
     CDS             complement(<1..832)
                     /locus_tag="ACG8FK_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5097012.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(846..1643)
                     /locus_tag="ACG8FK_001398"
     CDS             complement(846..1643)
                     /locus_tag="ACG8FK_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5097013.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(1676..1960)
                     /locus_tag="ACG8FK_001399"
     CDS             complement(1676..1960)
                     /locus_tag="ACG8FK_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS5097014.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1957..2958)
                     /locus_tag="ACG8FK_001400"
     CDS             complement(1957..2958)
                     /locus_tag="ACG8FK_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS5097015.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2960..3862)
                     /gene="gnd"
                     /locus_tag="ACG8FK_001401"
     CDS             complement(2960..3862)
                     /gene="gnd"
                     /locus_tag="ACG8FK_001401"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS5097016.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            4026..4874
                     /locus_tag="ACG8FK_001402"
     CDS             4026..4874
                     /locus_tag="ACG8FK_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5097017.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            5520..5726
                     /locus_tag="ACG8FK_001403"
     CDS             5520..5726
                     /locus_tag="ACG8FK_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS5097018.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5928..6929)
                     /locus_tag="ACG8FK_001404"
     CDS             complement(5928..6929)
                     /locus_tag="ACG8FK_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5097019.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6934..8846)
                     /locus_tag="ACG8FK_001405"
                     /pseudo
     CDS             complement(6934..8846)
                     /locus_tag="ACG8FK_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(9014..9520)
                     /locus_tag="ACG8FK_001406"
     CDS             complement(9014..9520)
                     /locus_tag="ACG8FK_001406"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5097020.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            9680..10120
                     /locus_tag="ACG8FK_001407"
     CDS             9680..10120
                     /locus_tag="ACG8FK_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5097021.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(10146..11261)
                     /locus_tag="ACG8FK_001408"
     CDS             complement(10146..11261)
                     /locus_tag="ACG8FK_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097022.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(11306..11662)
                     /locus_tag="ACG8FK_001409"
     CDS             complement(11306..11662)
                     /locus_tag="ACG8FK_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097023.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(11960..12934)
                     /gene="sgrA"
                     /locus_tag="ACG8FK_001410"
     CDS             complement(11960..12934)
                     /gene="sgrA"
                     /locus_tag="ACG8FK_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS5097024.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(13130..13969)
                     /locus_tag="ACG8FK_001411"
     CDS             complement(13130..13969)
                     /locus_tag="ACG8FK_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5097025.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            14154..15041
                     /locus_tag="ACG8FK_001412"
     CDS             14154..15041
                     /locus_tag="ACG8FK_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS5097026.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(15635..16330)
                     /locus_tag="ACG8FK_001413"
     CDS             complement(15635..16330)
                     /locus_tag="ACG8FK_001413"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS5097027.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAIKLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(16314..16712)
                     /locus_tag="ACG8FK_001414"
     CDS             complement(16314..16712)
                     /locus_tag="ACG8FK_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5097028.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         4996 a         3409 c         2574 g         5745 t
ORIGIN      
        1 aaaatccgtc atcatagtca tcttcgtcat caaaagatgc tgaattgtta ttgcttgtgg
       61 caggctgtac ttgtttccct ttttgcgact ggacaaagat caaagcgata ataatcccaa
      121 aaatggcata agtaaccatc gtaatatcta aagatttcaa aacaacactc ataaaataag
      181 caaggaagaa aaagaccaga taatcttttc gaccgataac gtatacagtt gtcgcgatac
      241 cgattgccgc taaccctcca cctactactt caagaatgtg gataacgact gttccctcta
      301 aagctttgat tacatcagcg atcaatggcg cccctaaata taaagcaatg aaaacaagcg
      361 gaacgaataa taaaaatgag gcaaccgttg gataaagaat aattgatcgg gccatagctt
      421 ttgtattcaa atcttctaca gctttgtctg tatatttacc taaaaaagta ttgatgaaaa
      481 aacgaaactg atacaagtaa cttccgagca atccgactgg caccgcaacg gcaatcgctg
      541 cttctggcga cagatcccct aataatgcga ctggtactgc gatagctgct gcaatagatg
      601 gttcagctgg cattgatcct cctggagaaa aaacccccat ataaatcatt tgcaaagcgg
      661 ctgtaacaat catcgcttgt gcaacttccc ccatgatcaa cccgacgact aatccagtca
      721 tcaaaggaga aaaacgtaag gtcaacgtgg ctcctccacc taaccagcgg gacataaccg
      781 cggctaccca taaagcaatc aacaaacttt gtactacact taatgtttcc atgtgtcctc
      841 ctccttcaga tctttctgat ataaccttcc agttccatca ttgactgtat cagtgatttc
      901 tgtgttacct ctataggaat caaatgcact ttctctttcg aatcaataaa ttcagccatt
      961 ttatctatta tctgtttatc tttcggaaat aatcccattt gttctagaga aaccggcagt
     1021 tctaattccc gatacaaagg atataactcg tctatttttt cccagcgacc ttctagtgcc
     1081 agttgataga aaatcccata cgctactttt tctccatgta gaaaacgatg gctttccgga
     1141 atatatttac tgatagcgtc atgcatagca tgtgctgccg catttcttgc gtatttatct
     1201 cctaatcctc caactaaacc agcaacagca aagataatct ctgaaagctg tttgaactcc
     1261 gatgtcagtt ttctttgtgc catatcttgg attgctttag cagaatgatt caaaatctct
     1321 tctttgcaga tcttcgcagt atcttgtgct agcttcaaaa atgattcatc ttgcaaaaaa
     1381 tcttgttcta agatgagctc agattcatac cattttgcta gtgtatctgc taacccggca
     1441 atgaaatatg gaacagggga atcaatcact aattggggat ctgttataaa aaaagcagct
     1501 tgtctgtaaa agtgttctgt tttcccttct gcttgtccat tctccttata catgacagca
     1561 agaggtgtcc atggagcaca attactagct aaagtaggga tgactccgaa atcgattctt
     1621 gcaagattgg cactataacc cactagatcg gtcagttttc ctccgccaac gccaactacg
     1681 aaatcgattt catttttttg atgattgtag ctattcgtct ggcttcagca taactacatt
     1741 ctccagaata acgatgataa aaaaattgaa tcgtttcgtc attcaaaaat actaatttag
     1801 gtagcgcctt ttcccatgat accgttccgt gggtgaccag tactcttttc gcgccgtatg
     1861 atttcaagag atcagaaact tttgaaatac caccttcttc atataaataa aattgagggc
     1921 ctgttttcac ttttaaaccg ttcaccattt tcttcatcat ccttttatat agttgctctt
     1981 tgtctggaca gaacgttcag gaagaaggtg ctgactgatc tgttcgacat ctctgacaca
     2041 tttttcaccc attgcttcta aacattccct tgtgtaggcc gatatatgtg gcgtaatcac
     2101 tacttgttca aacgctaaat acggatgatc ttgacgtccc ggttcttctt ctaaaacatc
     2161 tgtggcatat cctgcgatct ttccgctttt cagaccatta atcatcgctt cttcatcaac
     2221 tagagcgcct cttgcactat tagatagata aacattttct ttcatctgat cgacttcttt
     2281 atcagaaatc atgtgatagt tctcttctgt caaattcgca cataaacaaa tgatatctga
     2341 ttgttccagt aattcttgaa gttctacttt ttttgcacca aagctttcga tgtataaatc
     2401 tgatttgtaa ggatcataag ctagaacatc acagcgaaaa ccgttacgca aaatttcgat
     2461 tactccgctt ccggtatttc ctgcaccaat cacaccaact gttttgttaa acagcgtcct
     2521 accgacgaac tccgcccgat cttcccatcg atcgtccttg actcttgctt gtgcttgtgt
     2581 cgtgcgtcga agcatggaca acaaattagt tatattattt tctgctacag catttcgttc
     2641 aactaaagca ggtatgatag atacgattgt ctggtgttct tttgctgctt ttaaatcaat
     2701 attgttgtaa ccaataccat gtcgggaaat caatagcagt tcatctttgt tttcaaaaaa
     2761 atctcgtcga aaaaatggcg taacacttgc aataatgata ttgaatcctt taagtaactc
     2821 cgctagttct tttcctggaa tctcttgatc aacagtaaag aaatgaacgt ctccaactga
     2881 ctttaatctt tccaagtggt gtggaaagac tcttccgaaa ctgctggaat taacgattgc
     2941 tatatgataa tctggcattt tactcaccct tttcaactat ttcatgtccg ccaaacccat
     3001 ttcttagtgc tgaaactact tttgctgaaa aactatcttc ttcctgtgaa agattcctca
     3061 tgaacaaact agttgcaatt actggaactg gaacagccat ttctagcgct gtttcaaccg
     3121 tccattttgc ttcacctgat gcagaaacga ttccccgata attacttaaa ttgggatgtt
     3181 cgtttaactg atcttccatg atttccatca gccagccgcg aatcaccgaa ccatgattcc
     3241 aaacacgtgc gacttgttcc aagtcaaaag catactcact tttttgtaca agttggaatc
     3301 cttcaccgat tgcttgcatc attccatatt cgattccatt atggatcatt ttcaagaagt
     3361 gtccgcttcc ggatgcacca gcatacagat atcctctttc aattgctaca tctttgaata
     3421 attgttccac ggatagaaaa gtttctttat ctcctccaat cattaagcag gcttgatgtc
     3481 gtgctccttc gattcctcca gaagtcccac aatccaagta accgatttca tttttctgaa
     3541 actcttctgc tcgacggatt gaatctttat aataagagtt tccgccttca ataataatgt
     3601 ctcctggtcc gagttttgga aaaagttttc ttaacatgtt ttcggttatc tctccagcag
     3661 gaagcattaa ccagatgatt tttttctgct caaaagtagc tagaaattca gagatctctt
     3721 gataagcagg aatgtcattt tctcttgcct gactaacagc tgtacttgaa atgtcgagtc
     3781 cttgtacttt gtgatggtgc tcttttaagt ttaaagctaa attcaatccc atttttccca
     3841 aaccaattac accaatttcc atgatagcgc ctccatttct tataccctca ttatacaaaa
     3901 aaattttttt gtaaatagat tttacaaaaa tattttttgt ttgatttttt cctactctcc
     3961 ttgtatcttt aacattaatg agaacggaaa aaatgataat cttattatga aaatgaggaa
     4021 ttctcatgag cgtctccata agactacaaa tcaaaacaat gtacaacgac ttcagcccaa
     4081 aagaacaggc tatcgccgat tatattttag aaaatccaag caaagtaagt catagtccta
     4141 ttagcaattt agcaagtgaa ctgggcattg ctgactctac tttttttcaa tttacaaaaa
     4201 aacttggtta taatggattt aaagatttta aaatggccat gttgatccaa gaaaatgatc
     4261 tatctgctat ttctatccac gaaaatattc agaaaacaga tactgagctc actatggcgc
     4321 aaaaggtgtt cgagtctagt atttcaactt taaatgacac aaaaaagttg ttacaacaaa
     4381 aggatctaaa aaaagcggct actattatca atgaatctaa acgtgtctat tttttcggtg
     4441 ttggcggttc agagattgta gcaactgacg cttatcataa atttttgcgt tcccctatcg
     4501 caacttctca cagcacggat taccatattc aattgatgga agcttcttta ttgacggaaa
     4561 aagactgtgc tgttttgatt tctcacacag ggcaatcaaa agaaacaatc catattgctg
     4621 aaaccgtaag aaaaactgga gcaaaaacaa tcgttattac tagccaagct aattcttccc
     4681 ttgctaaact tggcgatgtc gtttttattt ctatttctga agaaacagaa ttccgatctg
     4741 aagctttagc ttcccgtatt tctcaactaa gcattttgga ctctctatat gtgattttga
     4801 tgttttacaa cgaaaaagaa tcacgtcgtt ctatcgctaa agttcgacat actatttctg
     4861 aaatcaaaga atagcaccat aacagaaaaa gcgaaaaatc cataaaacaa aaaaagccac
     4921 taccaaatat ttggtagtgg cttttttggt tctgcaaggg tggtacagaa catcaatagt
     4981 cggtgggtta gaacgtatgt catcgcatct ttggagaagg ttgtattggt attattcgag
     5041 ttacttgtta gatgcgataa caaagttagt atacagtagt aaaaaattta tcttttcttt
     5101 tttttattat ttttgtttct tattttctaa aaaattaaaa aaaattttgg tgaattaagt
     5161 atttccttga taacactggc ttttggcctc attttaggtt atcatttgta attttatttc
     5221 acagttgata tgattgttat gtaaaataaa aaagtttagg atacatcgaa tttttgggga
     5281 aattcttttg ggggtatcct aaatcttttc tattatattc ccatattttc tattattggt
     5341 ctagacgtat gatggtaaac tttgtaccta tctgattgct gaatcattaa ttgcttatca
     5401 ttagccatct ttgtttcaag ttcgctagat gatcatttta ttcacatcct aacaaacttg
     5461 tcacatttat agattcttaa tctatttatc atacaattat aaaaaaggag gtgctgctta
     5521 tgaaagctat ccatatttct ttgctttttt tcgctagtgc attgatttta tttgctattc
     5581 atctttgttc ccagccttat atcaataaca acggaatatt agtggaacca gcattctttt
     5641 gcttaccact cggctttttg tcccttcttg cctccgcagg cttcggattt gttgcttttt
     5701 ttaaacgatc aaaacaacaa atctgactta taaaatttac gtaaaagaga tcgtgacaat
     5761 aatcgtgtga tattcccaaa tttcgaataa aaggtgcccc ttgccatctc taactcctca
     5821 gtcacaagtt acaatattca taaaccatga aaagcttgtt tattgaaatt gctccttgtg
     5881 acttggagct aagtgacaga ttacaacctc ttttgcatca taagacttta ctccttgaaa
     5941 aacattacta acttttgata tgtttcttgc gcttgtttat tgctgaaatc aaactgatat
     6001 tcatggttca aaacagcatt tttctccgca taatctactt ttgtgacttt cacccccgct
     6061 ttctcaaata cagaagttac ttcttccatt tgtttcgtaa acgtatttgt atttccatcc
     6121 gttaagaatg tgttttgcgt ccaacgatcc gcatgtctga ccaaggctaa acttttgacc
     6181 ttctctgatt gttcaaagtc cttattatca aagtaagccc atccacattt ttcataaaga
     6241 aagctagaga ttttatcact tacttgagta agtgcagaag gatccaacag accagacaaa
     6301 gaaataaact tcgtaatctg cttgttctcc aaagtcggat caagatgatc agctttttta
     6361 atttcttcat tttgaagtgc taacaaatat tctccactaa tttgtgcacc tgccgaattt
     6421 ccaccaatcg aaatattcga ccaatctgct tgatctttta tctctttttt agctgcttga
     6481 atggcttcgt tcaattggat aactggtgta ggatgttttg cttccggtga tcgttcataa
     6541 tctatattta cgatgatttg ctgcgtgtct gaacatagat attccaaata atcacgacaa
     6601 tcatttttat cccctcctac atatgcgcct ccatgcatcc agaaaagaat cggtaatttt
     6661 ttcttttcca ctttcggtgt gtagatatcc atattattat ttttgtactt tgaaggatag
     6721 gatatttctt tttttatatc gatttgttcg attttttgct ggaaatcatc tggtcgggca
     6781 tatacttgtt ctttttcact tgtgtcaaat tgttttctaa caagcaagct aatcggcttt
     6841 ggtgtgattt gattaatcaa aaatacagtg actaaaataa ctacgatgat aactggtact
     6901 gcaatcacta ttcgcttgaa ccatttcatt catttaccct ctctttcttc gccaaatcca
     6961 tgtgataatc gtgctaacag ataaaaatcc acctgaataa atgagaagcg aatgtttctt
     7021 ttctccagtt ttcggcaaca ttcttttttt gcatctgtct ttttttgtac tttcactgtc
     7081 atctgaatcg gttgcgtatc atcacttgat tgatcctctc gaggttcatt ctttccctta
     7141 ttgtctgcat tctctgtcgg atcttgcttt tttggtggag ctactgggat ttttttgtaa
     7201 tagaaaataa tagttggtat cgtctcatta aaaatccctt cagtcggtcc ttcaacattt
     7261 gctaattcgt atccttcgaa tgtcttttgc tctgtcatat aattttctcc ataagctccg
     7321 taaagctgga tttcgtcctg gtctttgagt agacttttat tgttttcatc cacatacttt
     7381 acgtttatct gatcatggaa cactactttc aaaaagtccg ttacatgttt cgtgtaggca
     7441 gcatggtcaa cttcctgtgc ctttccatgc cctgctcctt ctactaataa aagatcttta
     7501 taccctcctg ataactcata taatttttgc gcgttagaca caggtactac atcatctgct
     7561 gttccatgga taatcaattt aggcaatgaa tctgacattc caatcgtagg tagagcagct
     7621 tttgtatcca aatgcatctc ctgatcaaaa tagtattgtt tcaaaaggcc cataaataaa
     7681 tcttcatgct cttgatcccc aatgatgtct aactgatttt ttatggcatc cggtagtgcg
     7741 ggggctactg tctggctgta cgtaaaacgt agttgttcat caattgatgc aaatccgcaa
     7801 ttttcaataa ttcctttgac attgacagga atatctttcg ctaatacaga catcatcgta
     7861 gctgctccca tcgaaccacc ataaagtaaa atcttttgag atggccgctc tcgaacttct
     7921 tgatttatcc agtaaaggac gtcatctgac tcatattgtc cataggtcac ataatcgcct
     7981 ccgctattac cagtcgctct ggaatcaaca aataataaat tatacccggc cttataaaaa
     8041 tcattgtatt cttctgtaac gatcccgtta ttccaattcc ctcgaaatcc gccatggatc
     8101 atgactgttt tatctgaacc ttgatcgaca taatatgcat atacatccat cattttgcca
     8161 tcttgtttgt ttagtacagt ttgatgaagt tgttttgtat ttggatagaa gcctgtgttt
     8221 tctgtaggcg ttgtcccatc atctgtacct ccaagcatcg acatcaacac actgtttttt
     8281 gcaaaagcga tatcgtacaa tagataagta gcaatcagat gtttgataat ctctttatct
     8341 acaccataat cattaacagc aagctcagtg actaataact ctattaattt tgacttgatg
     8401 tcttctgttg gtccattaag tatttctgtc atctgtttac cgaagtaatt caaatattct
     8461 ttaccattga aattttcacc tttaccgata tcatcatttt ctgtcccatt ctctttgtca
     8521 cttactttag cagaagagga ttcgtcgttt gacatttttg tagctgttgc ttcttctgtt
     8581 tgttggttat ctgtggtagc ggtcgtttct tgatctgtgc tagtaacaga tgtctcaacc
     8641 gttgtttcag tatttgtttc tgtttgtgaa gtactcgatt tcgtttcatt tccagcatca
     8701 gtagtttctg tggtttctac ctcccgagat tccgattcgg aagaaatgct ctctccatat
     8761 actgtccatt gcgggacgct tgtacttaca gcaaataaga tgaccaacgc tccctttaat
     8821 acttgttttg ttctcaagct attcactcct ttttatgttt ctaggagaat tatatgtaaa
     8881 cgcaaacatt ttttatacaa aaaataaatg gtttatccca taaaaataga acaggattat
     8941 tttctcattt tacgtaataa agcagaatgg ataaaccagt atccattctg ctttttttaa
     9001 acgaagaaac aacttattgc tccaaaaatg tcagaaatcg tttatcccaa attttctcgt
     9061 aaaaagcgat tgtttccttt gctttcataa aaggattatc ataagttgtc gttgctcctc
     9121 tacacatcat aagctggtat cctaatccat gagcaaactt cacagtcgta tctatacaat
     9181 actctgcttg tgccccacat aattcgatcg aatgacagtc caattcagtt aataaaacac
     9241 tcaaattagt cttataaaat gaattcgcat gggtttttct cacaaaataa ctgtgctcag
     9301 gaatagataa ttccgacaat agctcccatg gttttgtttg tggcactaat tgagcatctt
     9361 cgtgttgaac gaaaataact ggtttcccaa tcgtttcgta tgttttgatt cttttattca
     9421 ctaatgctgt cactgtttct aattcgtgta tcggcgaaga atcaaaacat accccttttt
     9481 gtaaatcgat cactactatt gcgtcggctg tgtttgacat acttgtgcct tctttctttt
     9541 ttcttgattt aatagtattt cttatctagc tagttccatt atacgtgaca acattttctt
     9601 attttacacc tctaaatggc acttttcgac tatttcttca tgttattatt tcataaatac
     9661 ttagctattg gaggaatgta tgatgattaa attgtataca ggttctggct gctcgtcttc
     9721 aagacgcgca aaacgttggc ttatcgtcca tgatataccg tttatcgaac agaacttaat
     9781 tcgtcaacca ctaaccaaaa atgaatttat ccacttacta aaattgacaa acaacggcgt
     9841 gaccgaaatg ctctctactc attctattag ttataaaaat cttgttcaca gaatagatga
     9901 actcagtttg agtgaactat atattgagat tcgtacatcc ccttctttat tgaaaaaacc
     9961 gattatctac gaccaatctg caaaactttt gactggattt agcgaagata aaatcaggat
    10021 gttcctttct aaaactaaaa gaaagcaaga gctcaacgaa ctcttgcttt ctggtcatct
    10081 tgtcgatttt aaagaaaata atctttttgc aatgtcttaa ttttggcata aacaatctgc
    10141 ttttcttaat taacgaaatc caatttttcc aatatggatc tatttttctc attcctgatt
    10201 tttatgattt cagctttcag acgcgcccaa tcattttttg taggttgtcc attccagtaa
    10261 aatatctgtg tattcgtttg atcatatgcc tttttgtcga taggataatc gctaatgaca
    10321 atatcaggta actgatcagc ggatgcacta tattttattg ggcgttcagt catagataat
    10381 atccttttct ttgcccataa ttcctgtgtt ttaccatact ttgattgaat atagaatatg
    10441 acaggttgtt cgtgttttat aaaaatatcc ctgactaaca tacagtattg aaaaatcaac
    10501 cgtctgttct tttcataata agtattaagc tctggaattc tgcgagctac cccactaaaa
    10561 aactgttgca tgattggata catcactgga aatatatgct gaaaatctct gtcttctgct
    10621 gattttccaa aagcatccac cttctttttt gtcccaaaaa cccttgaata caaatggatc
    10681 attgccaaat tgacatatag ataatttttt tctgaagttg taaaacaaat cttgaagttt
    10741 ttttcaaaat tttctacaat agacgattca atccagcccc aactttcttg tactttatct
    10801 ggagtatcca acaaatcttc ttccaaatag gtgttcatta caccaaacat aaaataaagg
    10861 taagcttgct ctgttggagt gatgtgattc atcgaccaga agtttgcttc aaaaaaaggc
    10921 tctagtatgt aatgcgtaaa ctcttctttt gttatcaatg gatgcacaaa tttgttcatc
    10981 tcttctggga catacgtaat cgagtggcct tgtttcattc gttgcaacga aattcttagt
    11041 aggctgatcc atttctttct atctatacta cgcgtatatg gcgcatgttt cgcaaatttt
    11101 tctaatagtt gacgtttatt ttttatactt gataatatat cagaatgatt ttcgtcgaat
    11161 gactgccaat acatcaagtg aaaaaagtat cggatacggt attcttctcc ttctatcgga
    11221 ttaccttttt tcaagctgca agataaatga tagtttttca aaatagtatt aagttctctt
    11281 aattttcttt gaatcgtacg caaacttata taattttttg actaagattt tctaaggatt
    11341 gataagtacc tttgtaaagt gaatccagta aaatataacc aatcgttccc tctaaaagta
    11401 tagaataaac tttactcagt aaaaactgag aagaaaactc gacttttatc atcttgtttt
    11461 ctgaaagaaa agataattta tattctggat acctttcgca aaacgattct gcttgttgaa
    11521 tcaccgaatt catcttagcc agggtgtagt ttagatctaa agcgatatcc ttcaccacat
    11581 ataatttctc gtctctttca taacaatagt tcaaaaattg atatacttgc ctttcattga
    11641 tatcaataaa ttcttcaaac aaagtacata ctccttttta tctattattt agacttattc
    11701 cgacagctac atgaataacg tacaaataga ttcgttactg atttttcaga gatactacca
    11761 tgcgcagtgt gcattgtcaa ttattcttgc tcttattttc catttgtgtt cctgttattt
    11821 cctgtttctc aaatatgtta gtaaacaaaa atagatctgg caaagctaca acttcgccag
    11881 gcaatacagc actctcgaat gaaatattca ctcaaaaatc tatcgtttta ttcaatttta
    11941 gatctgttgc ttttattctt tatttcactc gatggaagag agaacgaaaa tagcttgctc
    12001 ctacaatcaa aatgagtaac acacccatcc atgtaattag cttgttcaag gttctactac
    12061 cagtttctgg taaatcgctt tttctattat ctgtttcttt cgctttttct tgatgatcaa
    12121 ttccacgaga aacaaaatca ggctgtttta gcacactttt taggttgctc cctgcatttc
    12181 tcttttgttc cttagttggt atgataggta ccaaatcaag ttcattattt acttttcctg
    12241 tgctgctttc gctgctcgtg cttcctgtgc tactctcact actcatgctt cctgtgctac
    12301 tttcactact cgtactctcc gtactgcttt cgctgctcgt gcttcctgtg ctactttcac
    12361 tactcgtact ctccgtactg ctttcgctgc tcgtgttttc tgtgctactt tcactgctcg
    12421 tactctctgt actgctttca ccgctcgtgt tttctgtact gctttcacta cttgtgctat
    12481 ccgtactgct tggcggtcca acaactgtcc aactgcttgg tttcaaattc tccgtgatcg
    12541 tcatctgtcc atcataaaaa acaatcaaat caactttatc tttactcttg atctcatcaa
    12601 tcgatagttc tgcagaaatc attattgtat actcaatacc agcggtccat tgtacgtctt
    12661 ttaaataaac ttccccaata actttttctg aacttccatt accatcccaa ccatatgtta
    12721 gaacagcatg tacatcacta gcattcacgc ttttgtttgg tttgactgga atagtaatga
    12781 cttgttgtgt catgtcataa acaatccgat cagttttcac agtaaatgaa agattctcat
    12841 ttgcccgttc atttgcagaa gtactgattc caatcattgc tgcaattata ctcagcccaa
    12901 gaataacaaa taatacggtc gctgttttct tcatttctat cactctccat ataattacct
    12961 tctatttgat aaaggaatta tataaaatga aaatgatgag aacacgacag tcagttgtga
    13021 aaatctaaca aaagatgtcg tgtattcgct tgaatttgtt tactttttcg tttaatcaat
    13081 gacagactta aaaaagcaag accgatatca gccttgcttt cttcatcttt tattgataaa
    13141 cagcagtgta tttcttcatc cgctttgttt tatatgcata gagtaacaaa ccaacaatca
    13201 agactaacaa gaataatgta gttgatatag atgcttcagc cccaaagttt cccccagtaa
    13261 ttattgtatt ttgcgattgg atcagagtgg agaaaaaacc ttgttcttgc gttccgctga
    13321 ctggtatacc caaaatagct ccttgtgtcc aattccaagt aatatgagca ccaatagccg
    13381 taaataaact tcctgtactg tagataagtg cgctgaaaaa gcaacctgct aaaaagatat
    13441 tgaaaactgc cagaaatgtc atgttaggat tcaatccgtg caaaagagca aataatgcag
    13501 cagatccaat cattgctatt aacggaccaa atctaagcat ctgatgctga aaaaagccac
    13561 ggaataaaag ctcttctgat gcccccactg caataaataa aataaaactc gttatcagcg
    13621 gcacaatgtt tgcgtttcct gaatctaacc gatacgcccc cgtcaaatat aatggactag
    13681 taataagcag aaacactcca attccaacaa cgagacctcc cgctaaatac ccccagtctt
    13741 ttcctcgaac agaaacacca taatcttcta ttctttttcc ttcgacctta ctagcaagaa
    13801 ataaagctag taaaaaaaca gctaaatgtc cgaccgtacc ggcaattgct ccaatccatt
    13861 cactatttcc aataaacata tccgcaagtg cattattgac aagaattcct aaaacaaaac
    13921 caactaagac tattaaaaca gatccaatca accgaataat cctgatcatc tttcatccct
    13981 cctttatatt aagtttaaca acccttttct ttcttgtctc gtgaaaacta tgtaaatgtt
    14041 ctccatcata gagagaatag atttttacga actaagaata tctacagcaa ccttacgaaa
    14101 atgaaagact gtctccctaa ttcttctgtt agactgtttt taataaggaa aggatgggaa
    14161 attttttgaa aaaacaacta ctaattatgt gcctttcagc atgtgcaatt ttactattct
    14221 caggttgtag caagtccaat gaaacgattg caacgtttaa aggtggaaca cttacagttc
    14281 aggattttta tgataaccca aaagttaaat caaataatca aacactatta aaaaaaatga
    14341 ttgtcttcaa agcatttgat gatgtatacg gaaaagagat ttcttcaaag gaagtaacaa
    14401 aagaatataa tgaacaaatc aaaaaactcg gtccaaacta caaggaacaa ttaaaagcag
    14461 ttggccaaac agaagagaca tataagaaac tctttaaaca gatgctggct ttccaatacg
    14521 gtttgaaagc caatgtaaaa ttaacagata aagatctaga cactgcttgg aaagaattct
    14581 atccagaagt tagtacacag atcattctat tttcgactaa agaagaagca gataaagcaa
    14641 aaaaagaagc aaatgaagga gaaaatttct ccaagcttgt tcaagcttat ggaaaaaata
    14701 aaacattaaa ggaaactgat ggaaaaatga attttgattc aaccaatcca gagattccta
    14761 ctgaagtaaa aaaagcagca tttaaactaa aaaacgggga agtttctgat atcattcctg
    14821 taacagatcc aacgacttat cagcaaagct attatctagt aaaaatggta aaaaaacagg
    14881 ataaaggttc taataaagac aaatacaaat ctgagctaga aaaaattgct accgaagcaa
    14941 aaactcttga tacggaattc atggataaaa caatccgtaa agtgatgaaa aaagacaatg
    15001 taactattaa agatccttat gtaaaaaata tattcaaata aacaaatttg acccaattgg
    15061 ggatgtggca aaatttgaga agtgattttt ggaaattcct tcttattttt gaggcaaacc
    15121 acttttgcca caacttcatc ggggcctttt tttatttgta ccaaaacaaa tcactggatt
    15181 tgttacaatc atgaggatat aatttaaaaa agttttttat ttctatcaca tgaatcaatg
    15241 cgccattaag tagattcttt catgcttccc tattttttca cttgccgtat cgcttcaaaa
    15301 acaactatca taaactcaaa ctcgactgaa aagttatttt aaaaaagact aaaaatatcc
    15361 cttctggact tgttcttctt attctgttat actagagaaa cacgaaagat atttttatgc
    15421 gacacttttc atttgttttt atgaaattta ttcaaaactg gcgtaacttc agagttacaa
    15481 ggatgtatta gaggttaggg aatacatcgt ttaaatgaga aataagtaat agttagtaat
    15541 agtcacccaa actttaaatt aatgaatatt gaatcgtcag atagcctcct cgagttttcg
    15601 agaaggctat cgtttatttt atggagaaat tgattcagaa gtaatcagcg acctttttta
    15661 gttcaccaat cgtttctgct tggaaagttt gcacccaatc ttcttggaag cccctcactg
    15721 ctttatcaat cgagggcatc tctaatactt gatctagtag ctctttacct aactttatcg
    15781 catgagctcc agatcgaatg gctgtagtga cttgttcaca atttttgaaa ctagctgcta
    15841 atatttttgt tggtctttgc gtacgttcga tctcagtccg caacgaatca atcactttca
    15901 tagcatcaat gccttgattt tgcatcctgt tataataagg tgcaatataa tctgctccat
    15961 tattaatagc taaataccct tgtaattctg tgtaaatagc tgtaccggta atcaaataac
    16021 cttctttttt caactttttg atcgcttgaa ggcctgcttg attcaccggc accttgataa
    16081 acgtatcttt tcccagttct tctatgataa ctcgagcatc ctctatcatt tcttcttccg
    16141 ttcttcctat tgtctgaata tggataggaa tctgtccgat tatttttttg atttctttca
    16201 tatgcgagaa gaagtttgac gctccttctt ttttcagaat tgtaggattt gttgttacgc
    16261 ctgttaaatt caaaaactgt tgacaagaat aaatactttt tgtatttgct gtatcaagca
    16321 tgatttccat atagctttcc cccattctga tcctcttgat cgattttaat gattgtcccc
    16381 tttcttaaat gcggcatttc cttatcttct acatacattg ttcctggaag ttccggctca
    16441 tgagatccat caaattttat cgtgatatgt cctaattcat tcaaattagt ttggacaaca
    16501 tctcctactg ctgtgattac atacatttgt ttatcaaaac agagcttcat atcacaagtt
    16561 atttcttttc ttacctgatt caattcaatc ttataacaat aatctgttaa tgtttccggt
    16621 gcttcatctc caaataaaat cattattttt tctttcctga atacttctgc ttctatacca
    16681 acatctgtta caaccgtttc aaagatcgtc atctctgttc ctcc
//
LOCUS       DAZUMV010000046        16705 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000046 DAZUMV010000000
VERSION     DAZUMV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.046"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..1583)
                     /locus_tag="ACG8FK_001415"
     CDS             complement(78..1583)
                     /locus_tag="ACG8FK_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5097029.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1580..2266)
                     /locus_tag="ACG8FK_001416"
     CDS             complement(1580..2266)
                     /locus_tag="ACG8FK_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5097030.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2459..3880)
                     /gene="gndA"
                     /locus_tag="ACG8FK_001417"
     CDS             complement(2459..3880)
                     /gene="gndA"
                     /locus_tag="ACG8FK_001417"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS5097031.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4050..4229)
                     /gene="rpmF"
                     /locus_tag="ACG8FK_001418"
     CDS             complement(4050..4229)
                     /gene="rpmF"
                     /locus_tag="ACG8FK_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5097032.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4287..4856)
                     /locus_tag="ACG8FK_001419"
     CDS             complement(4287..4856)
                     /locus_tag="ACG8FK_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS5097033.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5042..5944
                     /locus_tag="ACG8FK_001420"
     CDS             5042..5944
                     /locus_tag="ACG8FK_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5097034.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(5979..7763)
                     /gene="pyk"
                     /locus_tag="ACG8FK_001421"
     CDS             complement(5979..7763)
                     /gene="pyk"
                     /locus_tag="ACG8FK_001421"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS5097035.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7837..8799)
                     /gene="pfkA"
                     /locus_tag="ACG8FK_001422"
     CDS             complement(7837..8799)
                     /gene="pfkA"
                     /locus_tag="ACG8FK_001422"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS5097036.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(8967..12281)
                     /gene="dnaE"
                     /locus_tag="ACG8FK_001423"
     CDS             complement(8967..12281)
                     /gene="dnaE"
                     /locus_tag="ACG8FK_001423"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS5097037.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12512..12697
                     /locus_tag="ACG8FK_001424"
     CDS             12512..12697
                     /locus_tag="ACG8FK_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS5097038.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12759..13955)
                     /locus_tag="ACG8FK_001425"
     CDS             complement(12759..13955)
                     /locus_tag="ACG8FK_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5097039.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14125..15561)
                     /locus_tag="ACG8FK_001426"
     CDS             complement(14125..15561)
                     /locus_tag="ACG8FK_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5097040.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            15854..16663
                     /locus_tag="ACG8FK_001427"
     CDS             15854..16663
                     /locus_tag="ACG8FK_001427"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5097041.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4891 a         3518 c         2936 g         5360 t
ORIGIN      
        1 ccctgaattt ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa
       61 caagtaaagc acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa
      121 attgtccctt gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt
      181 tgtttagcga tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc
      241 actcgataaa aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag
      301 tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt
      361 cgtgtagttg aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga
      421 ttacgataaa tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag
      481 tctggatata atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt
      541 gtttcattcg cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga
      601 actaagcttt tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc
      661 tcaggatcat ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca
      721 ggtgtcctta actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa
      781 cgcatacggt caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg
      841 tcgatttcag gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct
      901 ctgagtacct tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt
      961 aagatcaaag aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca
     1021 tagaaagaag ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta
     1081 gaatatatag gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga
     1141 actttacgtg tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc
     1201 tcgtccaaat cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa
     1261 tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca
     1321 tttgtcgctt tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta
     1381 gccaaacgag aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca
     1441 atcagattga tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg
     1501 aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt
     1561 tccgattttt tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga
     1621 tatagctttc ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca
     1681 ctacgtttgt ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg
     1741 ctaataccac gtttacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta
     1801 gttcaattac ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc
     1861 gataagtaat agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca
     1921 acaatgcacg aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat
     1981 ctgctccgtg gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa
     2041 tgattggtgt atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg
     2101 ggagcatcag atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag
     2161 ttcggccgtt gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa
     2221 atcgtgccaa gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc
     2281 tttctattct actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc
     2341 tatagtcatt ttatcatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat
     2401 cgacttcagc tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt
     2461 attcttcatg gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat
     2521 gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt
     2581 atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag
     2641 ccaaggcaac gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat
     2701 catctagcaa taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca
     2761 agaatcgagc tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca
     2821 agtcccaatc aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca
     2881 tgattttact gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg
     2941 tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat
     3001 aagcagaaat atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt
     3061 caagcgcgct ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca
     3121 catctactac tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga
     3181 tttctatcaa atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt
     3241 cttctactga aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca
     3301 tatctccata ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc
     3361 caatatatgt gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg
     3421 gtgcaactag cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag
     3481 ctccttcttc accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact
     3541 cttcattacg acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc
     3601 ctttatcaag gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga
     3661 ccattagcaa gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg
     3721 ttgctttgaa atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac
     3781 gattgaataa agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca
     3841 taacggccat accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg
     3901 ggatttctcc ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac
     3961 aacatatttt ttatttaccg actacaaaaa aataaacctc aaggctccat ggttagcctt
     4021 gaggttttac attttctaat taaaatgtat taagcttctt ttgacattac gtccttacca
     4081 tcatagtaac cacaatttgc acatacgtga tggctttttt tcatttcacc acagtttgga
     4141 cattcgttta accctttgat tgtcaatttg tagtgtgtac gacgttttgc ttttttagct
     4201 tttgatgttc ttctagctgg tactgccatt ttgctacacc tccttgtaaa gtcttgtatc
     4261 taaattagac acaatatatt ccaatcttac ttgttatcgt cgtcttttga tttagaatca
     4321 aaaagttcag atagttttgc aagacgagga tcaattttat tctcggcttc ttccattttt
     4381 ctgcgctggt attcttcttc ggaaacaact tcccaatcat tgccttttgg aagttctgtt
     4441 gctgcttcct cttcttttgt caatacccgc attggaatcg ccaataagat gttgtcttca
     4501 atcgactctg ctaaattcaa taatggacct tcaattagca aaatctcatc tgcagacatt
     4561 tgttcatctc tactttggaa ttgttcttct gtcatgaata cttcgtctac agaaaaatcc
     4621 atgggcagtt caacaggttc tagtgaacgt gttgaaggaa ctgtcaaagt cgtctgcaca
     4681 gtgtaatgaa cgatatagcc tttcttatca actgagacca aaccttcgac ttccactggc
     4741 gcaacatcaa gtattaaatt gtctcgcttc atgagagcgg ctttcaagtc gagcgtctca
     4801 tgaaaagtta acggtgtctc ctggtatttt ctcaattcca ataaagacca tttcattttt
     4861 catcactcct aagcaacaaa agcaattata cagggcaaaa agcatgttgt caatgaaatt
     4921 ttcttgacac cttgcagttc ataaaaaaac agaaaaatct ttattagaaa gcctctgttt
     4981 gctatcatcc tcagaatcat ttacaataat cattattcca ttaaaggagc ggatgatctt
     5041 catgaacacg aaagatatcg aatactatgt ccaaatcgtc aaacaaaaaa attttacgaa
     5101 agtggctaat tatttccgtg tgagccaacc tactattaca tatgcattac aacgtcttga
     5161 aaaagagttt gacaccgtac ttattacaag agatcgatcg catcacgaat tgatcgtcac
     5221 gccttctgga aagcagctgt ttctccatgc acaaaccatt ctccaagaga tttctactgc
     5281 aagagctgaa atcactcagt tgcaaaagaa aaagcttcgt tttggcatgc ctcctattat
     5341 cggcaatgaa tactttccta aactgtcaag ttactttatc aagcatgaat tgatgaatca
     5401 aattgagatt accgatggag gctctcggga cttatacggg atgatccgac aagggaggat
     5461 cgatttagct gtattaggct caacacagcc aatcgtggat caatatttag aaacagagtt
     5521 acttttggac aaacgtttca tgatcgtcgt gccacctaca catcctcttt ccaagcagga
     5581 tactgtctct ttcaaagata tagcagatga acagtttgta ctattgaacg aacattatgt
     5641 ccaccccgca gcattcacta agctttctaa acaaacacat actcatccaa aagttgttta
     5701 tcaaagcaat gacttgacga ttttaaagag tatggtccga gaaaatgtgg gtatcggttt
     5761 tttggcagag attgctgtcc atcctgaaga tcatttagtt tgcctccctc tttcagatga
     5821 agttcagcct cgcttcttga tttcattggc aaaacgaaca cagcagatcg tcacgcctct
     5881 tcaagaacaa gtagtccaag ttatccatga attcgttaca gcacaaaaaa acagctcttc
     5941 gtaaaagaag agctgttttt attttttatt aagctaaatt agatagctgt tgtttcgcca
     6001 cggtaaacga tgccgcgacg tgaatcaacg gtaatcaatt caccttctgt aattgttgtt
     6061 gttgcgcctt tagcaccaac gattacagga atatcttgtg caattcctac aactgcagcg
     6121 tgagaagtca atccgccttc ttcaacgata acagcagctg ctttttcgat tgcaggcata
     6181 tattctttgt cagttgatgg tacaactaga accattccgt cttttgcttt ttttacagct
     6241 tcttcagcag atgaagcaat aactgcattt gcaacgacag atttttgtcc aacgccttga
     6301 gcttctaata atttagaacc aatcatttga attttcatca cgttagttgt tccgcgttca
     6361 ccaactggta caccagcagt gatcaagatt aaatcgcctt ctgatgcgaa acctaattca
     6421 acagcttttt tagctgctag ttcaaacatt tcatcagttg ttgatggttt ttcagcaact
     6481 gttgggtaaa cgccccagtt taacatcaaa ccacgttttg tgcgttcgtc aaaagtaaca
     6541 gccaagatat ctgcatctgg acgatatttt gaaatcattt ttgcagtgta tcctgattca
     6601 gttgcagcaa cgattgtttt cacgcctaga ttttttgcag cacgagctac agataaaccg
     6661 attgtttcag ttacatctgt tttatcgaat tcgttcaatt ggaatgtacc taattctgat
     6721 aatgcatttt cagcttcttg gtcgatacgt gccattgttg aaacagcttc aactgggtag
     6781 tccccgtttg cagattcacc agatagcatt gttgcatctg tgccatcaaa tacagcgtta
     6841 gcaacgtcag aagcttccgc acgtgttgga cgtgggtttt cttgcattga ttctaacatt
     6901 tgtgtagcag tgataactgg gataccagca gcattacatt ttttgataat gcgtttttga
     6961 accattggta ctagttcagc tgggatttca acacccatat ctccgcgggc aatcataaca
     7021 ccgtcagaaa ctttgatgat ttcgtcaatg ttgtcgatac cttcttgaga ttcgatttta
     7081 gggaagattt gaacatgagt catgtctttt tcttctaaga tttcgcggat atctagaacg
     7141 tcttgtgctt tacgtacaaa acttgcagcg ataaagtcga tatcgttgtc taaaccaaaa
     7201 cgaatatcgt cagcatcttt tggagtaata cctggcaaac taatagaaac gcctggtgca
     7261 tttacccctt tacgtgatcc tagcatacct gcatttttaa cttctacgac taattcacgg
     7321 tttgcttcgt ctttttcaat gatttgcata tcgatcaaac catcatcaaa taaaatgtgt
     7381 ccgccaacgt gaacatcgtc aaataatcct gggtaagtaa ccgcgatttt ttcttttgaa
     7441 cctacatgtt caggatccat agagatgcgt gtttgatctc ctacttcaaa tttgatgtat
     7501 cctgcacgac caaaatcagc ttctgttgtg ccttgaacag ttgtacggat ttcagctcct
     7561 tttgtatcta aaagaatacc gacatctttt cctgtctttt taactgcttc acggaccata
     7621 gtcatacgtg ataattgttc ttcatggtca ccatgagaaa aattaaagcg gaagacattt
     7681 gccccagatt caatcaattg agaaataatt tctactgagt tacttgctgg tcctaatgta
     7741 ctaacgattt tggttttttt catttaaaaa actctcccat tcaattttgt ttatttaaag
     7801 ctgtacacag cttaaatagc ttggtttctt tcactcttaa aatgaaagtg aattatttag
     7861 atcatatagt gataagtctg gtttatgttt gttgttttca agtgtatcaa tgatgtcaga
     7921 cgcaacgatt tcgttgtcat ggataccgac acataatcca ccttttcctt cttgaagcaa
     7981 ttcgacagcg taagcaccga atttacttgc caacacgcgg tcgcgtgcgc ttggtgctcc
     8041 accacggaca acgtgtccca agatagatac acgtgtatga tagtcaccaa attctgaaag
     8101 tttctcagcg aattcatttc cgcccattac accttcagcc aagatgatca agcaatgttt
     8161 cttcccgcga tcgcggcctt cttggatttt tttagcaact gaagccatat caaaatcatg
     8221 ttctgggatg atgatttcat ccgctccgcc agccacacct gaccataaag cgatgtctcc
     8281 tgcattgcgt cccattactt cgataacgaa tgtacgaaca tgagaagtcg ctgtgtcacg
     8341 aatacggtca attgattcta gtactgtatt gattgctgta tcaaaaccga ttgtgaaatc
     8401 agtcccagga atatcattat cgattgttcc aggaatacct actgctggga aaccgcgttt
     8461 tgtcaaagcc attgcgccat gataagagcc gtctcctccg ataaccacta gaccttcgat
     8521 tccgaatttt ttcaattgtt cgattccttt taattgacct tcttcagttg caaattctgg
     8581 ataacgtgca gaatacaaga atgtaccacc acgttggatc ttgtcaccta catctgccac
     8641 atcaagacga cgaatatctc cagcaactaa accggcaaaa ccataattga tcccgtatac
     8701 ttccagtcct tcgtaaatac ctttacgaac aacggcacga acagctgcat tcattccagg
     8761 tgcgtctccg ccactggtca aaattccgat gcgtttcata ataaacattc acctcattaa
     8821 aatactttat cctaagtatg acttaggttt acaaccattt ttgcgttaac cacgactaac
     8881 attctaacat tatttttctc acttgtcttg atttacggat aaaaaaatga aaaaaaatga
     8941 aagcaagtag tattttttac cgtaagttat ttgaaaacca cattatcttc gccaagcaga
     9001 taaataagtt gccgctttat actcttgctg tcatccaccc aataagcatc tgacagaact
     9061 acttttcgtt gcgttttttc gtgatacata actactggta tatatcctgg gtgtttctga
     9121 aaaatcactt gtaattgttt cattatctcg ggatcttccc gttcttctgt aatacgcaaa
     9181 tagcaagttt gtgaaggata ctgggcttct aactggttag gatcagcaaa gctttctcca
     9241 attagttgca gttcctgatt atatttgctg atttcgctcc gcccttgaac aaagatcact
     9301 cgatcaacgg caaacaaatt acgatattta cgataaagag ttggaaaaac tgtgacagaa
     9361 atttctccac tactgtcggt tccttcaaca aaggccatca gttctccttt ttttgtccga
     9421 atttctctaa cttccttgat catcactaat aaattgactg gttgattagg gacgatatct
     9481 gtaatcagct gaacttgttt agctaaacga agacgatcaa acccttctgc aggatgacct
     9541 gacaaataaa tacctaagta ctcctcttcc agcttcaagc gttcttctag tgtatagtca
     9601 gcgacttctt cttcttttaa tgccatgatt cccagcaagt caagacttcc accactgtaa
     9661 acgatatttt ggatttttcc ttcgagatca agcatcaatt gtttcctgtt aggagataat
     9721 tcatcaaaaa ctcctactgc tgtcagtgat tgcagtaact ctaatttcag ccaacgctta
     9781 tcgatccgta tgaggaattg atccactgac tggaacgggc cattttgttt tctttcgttt
     9841 aatatatctt ctacaaaatc acgccgaata ccttttatcg ctgtcagtcc gaatctgatt
     9901 tcttccttat tgaccaactc aaaactggaa ttactttgat taatggacgg cgaggaaagt
     9961 ttgagtttat ttttacgtgc ttctgcaata tattctttca attttgatgt gttatgccga
    10021 acagaattca tcaggctggt aaagaaagca cctggatgat gggcttttag atacgccatt
    10081 tggaatccca cgaatgaata agcaaatgca tgtgaccgat tgaatccata gtttgcaaat
    10141 ttttcgatat attcatatac ggtcgctgct gttttttccg aatatccctg ttgtaatgct
    10201 cctgatacaa aatgcgactt ttcttcatct aatacgtctt ttttcttttt actcactgcc
    10261 cgtcgcagaa tatctgactg ccctaatgta aatccagcca tagtcgaggc gatctgcatg
    10321 atttgttcct gatagacgat cactccatat gtgtttttca aaatcgggat cagtgaatca
    10381 tctgggtagc gaatggcttc ttttcctttc ttcctggcaa tgaaggtatc tatattttgc
    10441 atagggcctg gtcgatacaa tgcattgact gcggcaatat cttcgatgtt ttctggatct
    10501 aatcttcgta aaacgttccg gataccagca gattcaaatt gaaatactcc tgaggtttcg
    10561 cctcgttgaa acaagagcag cgtttttgca tcatttagcg ggatctcttt taatcggatc
    10621 tcataccctt ctactcgctt aacagctttc aaagtaaaat caataatcga taagttgcgt
    10681 agtcctaaaa agtccatttt caaaagaccg attgcttcta cctcattcat cgtatactgg
    10741 gtcagaaaag cttcatcaga accgcgttgg agcggaacta gttcaagtag attcttatcg
    10801 ctgataacta cacctgctgc gtgagtcgaa acatgtcttg gcaatccttc taaaatcact
    10861 gcagttttga ataaaagttg gtttttttcg cttgcctgaa ctaattctac taattgatgc
    10921 gaagttttat aagcttgggt caatgtgatt ttcaattgat tgggtattgc ttttgaccag
    10981 cggtttgctt cactttgcga taaaccaaac actctagctg catctctaag aaccattttt
    11041 gctgccattg tgccaaatgt tgcgatttgt gccatatgat attgtccata cttttctcga
    11101 acataattca atacttcttc acgtcgattg tcaggaatat ccaaatcgat atctggcatc
    11161 gtaaagcgtt cggggtttaa aaagcgttcg aacagcaaat catattctaa tggatccaca
    11221 tctgtgattt ctaacacata ggctactaat gaacccgcag cagaaccacg acctgcaccg
    11281 gtaacgattt tgtttctatg agcaaaagcc attacatccc atacgatcaa gaaataatca
    11341 tcgaatccca tttgatggat gacagataac tcgtagtcca aacgttcttg gtattccttt
    11401 gtatagtcag tgattcggcg ttccacttgc gaatagcata actctcttaa atattctcca
    11461 gcttgttttc cattaggaac tggataatgc ggaagtaatt tctgatggaa ctggaattca
    11521 aatgaaatcg attcagctaa acgctgggcg cctattacag cttgggaaag acccgctttt
    11581 tcgaatcgtt tgatcatttc ttgttctgaa agaagataga catttgctgt tagtggtaaa
    11641 gatgcagata aattttctaa aggtatttgg cttccctctt tgatatgttt catcacttca
    11701 agtgcaaaaa gttcttcttg ttgtaaacta gcgatgggat gtaatgcaca aagcgaataa
    11761 ttttctttta tcacatactg ataccattca ggcaattgat caggcgtgtt ttctgtcaag
    11821 ccgatataaa aatttctagt aaaaacattt tcaagtaaaa actgccaatg accgaaaaag
    11881 ttatcctctt tattgaaagc ctcagaaaca gaatgatttg ccggaacaac aaacgctaga
    11941 tggtcggttt ctgtcatttc tgcaagttcc aatttttctt ggatcatttt tcggctggaa
    12001 agtttcatca gctgctgata acctatttca tcttttgcgt acgcaagcaa gccataattt
    12061 ttttcttcat aagtataact aagatgtaat ccaatgattg gctgtactcc ttcttctaca
    12121 caaatacgat agaattcagg tgcaccgtat aagacatctt tatccgttat ggctaatgaa
    12181 gtgtagcctt gtttttttgc ttcttgtaca tattctcgaa tacgtatcgt actttgtaaa
    12241 agagaataag aagtaattgt ataaagttgt gggaaaaaca tcttgcattc cgcctttttt
    12301 ttcgttaaaa ttcactagcg ttaaaaggtt tgatcctttt actttcatta agatagcagg
    12361 cgctgaagtt gattattcca cttcatttgc ttgcttgtaa tctgtaggaa tgagttcttt
    12421 cgcaagaact caaattttat tgagatttgg tctcgtttct ttacattcaa agaaaatctg
    12481 gtaaaattaa gatagaaaag aggtgttctt tatgcgatat atcgttacat tattctgggc
    12541 tgtcgttcta ggtcaagtag tcggttatat tggtgctgca ttaacaagcg gaacatatga
    12601 ttttactttg acaacaatca tttcattcat tgcgggtgtc attatccttt tgattggtgc
    12661 agttgcgcca agaaaagaaa cctctgccca ttcgtaaaaa aacagccaag agcttgagat
    12721 ttctcaagct cttttttatt ttctactcct caccaccatt ataatctgct gcgaagaact
    12781 ttacgaaagt tgaataaaga aagaagaata tttgacagaa caaacaaact tgtcacaata
    12841 aaaacagcac tgtaattata agcatgcgcg actgtcgatc ctagcatggg acctaaaact
    12901 tgtccaaagt tgctaaacat ctgattgtag ctatagattc ggctgactcc ttcttgaggc
    12961 gtaatcttac tgatcagtgt attgacagaa ggcatcaaag cgccagttga aaagcctaaa
    13021 ataaagcgaa gtatacctaa ttggagcggt gtagtgacga atgccatagg taaataacag
    13081 caaaatgata aaatgagtcc tccaattaga actttttgat tcccaatctt atcccctagt
    13141 tttcctagtt tcggtgaaga aaagacagcg gaaacaccag ctatcgaaac aatcaagccg
    13201 ctgacaaaaa ggatattttc tgtacttccg ctcaattcac gaatatataa ggtcaaaata
    13261 gggctgatcg tagtgattcc tatctgtaaa atcaaggtcg taatgaagag accgatcaaa
    13321 atagagagat gatccatttt tgaaaaaatt tctttggtgc ttatcagatc ttttttctcg
    13381 attggatgga aatcttcttt gactaagaaa atggtcaata ccgttgtgat catcaacaaa
    13441 gcccctgtga tcaagaatac attttccata ccaaaccact gagccagtgc accgcccatt
    13501 gaaggtccga tcagatttcc agcaatcgct ccggtagaca atgttcctaa cgcccatccg
    13561 ctcttttctc taggtgcctg tgaagcaatc atcgctgtgg catttggaat atatccagac
    13621 aaaatcccat tccaaaaccg catgatcaaa agccaatacg cgtttggtac aaaagccaaa
    13681 gatcccattg ttaatgtcat ccctgctgct gcacggatca tcatgagttt tcggcctttt
    13741 cgatcagcta gatttcccca gattggtgca accacggcag atgcaaaagc agtcacagag
    13801 attgctagtc ccgaaaacaa ttcgatttga tcttttggtg ttcctagttg ctcaacataa
    13861 actggtatga atggcataac taaactaata cttgcaccag tgaaaaaaca gccaatccac
    13921 gcaatcacta aattttttct ccaattaatc tccatctatc atccctcttt cgtctctttt
    13981 atcataccta ttttcgaata aacaagcaac gatctcgcca gtaaaaagag aaaaatcccg
    14041 caaaggtcat tttatatgta acgttctttt tatgtaaaac aaaaagagat cagaacaaat
    14101 actgttccaa tcccttctct ttagttaaat tgttgctaga atgatgaagt tgataatgaa
    14161 taaaatatca acgatccaca aagcaacaga aacttgattg tattttcctt tgattacttt
    14221 cacaattgta tagaaaataa atcctgctgc aatcccatag gaaatgctat agcacaatcc
    14281 cataaagatc gatgcaaaga aggccggtaa agcttcttcc atatctagcc atttgatgtc
    14341 tgcaaaagaa gccatcatca ttactccaac taaaatcaat gctggcgcag ttgcttgatt
    14401 tggtacaatg gcgatcagtg gagagaataa actacttaat gcgaatagca ctgccactac
    14461 tactgaagtc aaaccagtac gtccaccggc cccgatccca gctgcagatt cgacataagt
    14521 tgttgtattc gatgtaccaa agatagcccc cacagaagta gcgatcgcat cggcaaatag
    14581 tgctttatcc atttttgttt tgaacccacg accatcttcc aacgcgatct cgtcttcttt
    14641 tgagaaaata cctgtgcggc gtcctgtacc gataaatgtt ccgatcgtat cgaaagtatc
    14701 agataaactg aatgcgatga tcgtcatcaa tacttgagga atcttggaag aatcactgaa
    14761 taaagattgc atgccttcag aaccaaatgc tgcaccgaaa gttgttccta attctttaaa
    14821 tgaattacct aacgaattcg tttgccaatc aatggaactt aaatcaacaa cacccataaa
    14881 aataccaagg actgtggtcg ctacgatacc gataagaata gccccacgga cattttttac
    14941 aactaaaatg gctgtcagta ccaaaccaat cacagccaat aagataggag cgttgtcaaa
    15001 gttggctaac gcagggacga ttccaccatt tatcgtaaca tttgtcgctt ttccgccttc
    15061 aacgacagag ctcgaaatag ctgactggtc tgctgaaaaa gataagaatc ctgcattttt
    15121 gattccgaca tacgcaacga aaataccgat cccgccacca atcgcatgtt gaagactttc
    15181 gggaatcgcc cgaataatca tttttcgaat atttgtgaca gtaataaaaa tattgattaa
    15241 tccgcaaata aacaccattg ccaaggcttg ttgccaagaa taaccaagcc caaaaacgac
    15301 cgtaaaagtg aaaaatgcgt tcaatcccat accaggtgcc tgagcatatg gtacatttgc
    15361 gaacaatccc attaccagcg taccaataat cgatgcaatg attgtcgcta gaaatactgc
    15421 ttggaagggc atacctgatg ctgacaaaat cgtcggattt acgaataaga tataactcat
    15481 ggcaaagaat gtcgttactc ccgccattat ttcagttgat acatttgtat tgtttttctt
    15541 taaaccgaaa aacttttcca taaaaagttc cactccctga atttttttct gatggaaaac
    15601 tataataata aaaaataagt aaaatagggc aaatccatcg aacaatttca aattatatag
    15661 acgtttcatt cgcttttcaa cgttttttta acaaaaaaag cgaaaataat acttaacgtt
    15721 cgtgtttagc ttatgtttag ctatttatat agccataact gtagtttttg ttaggaatag
    15781 ctgattgctc ttgttaatta cctaaaatat gctagactaa aacaaatgct agttgaggaa
    15841 ggaaggaccc ttattgaata aaaaattaat tgccatcgat ctggacggaa ctaccctcaa
    15901 tcaagattcg atgattacat ctaaaacaaa agagactctg aaaaaagcaa tcaatgccgg
    15961 tcatcatgtc agtattgcta caggacgccc attccgaatg agccatcagt tttaccaaca
    16021 attagaatta acaacaccga tggttaattt taacggcgca ttagtacaca tccctaatca
    16081 gcactgggat ggtgaaagag aaacgttgat caatcgtgaa atcgcttttg aaatcttatc
    16141 gcaaaaaaag cagctaaatt tagattttat cgctgctgaa aatcgtgata ctttttttat
    16201 tgacagtttt gatttctttg atgaaaaaat ttttgcttct tctcgtccag gagagaagaa
    16261 tttgctttct cctaaaaatt tgacgacaaa tcctacatct ttgcttgtcc gaacagacaa
    16321 acgttttgca ggagctgtat ctgctgaatt aacacgtcaa tttggcagtt atgtcgatgt
    16381 tcggacatgg ggtggaccta ctgccatctt agagattgtt tcaaaaggaa tccaaaaagc
    16441 aaaaggcgtg caagagattg ctaactacct gtctatcgat caaaaagacg tcattgcctt
    16501 tggtgacgag cataatgatt tggaattgtt ggattatgca ggttggggag tagctatggc
    16561 taacggtact gaccaactca aaggcattgc aaatgatgtc actcctttga gcaatcagga
    16621 agacggttta gctgtttatc tggaaaaact acttaaacta taatagagaa ttagattcca
    16681 caaaaaagag gttgtgacaa ttttt
//
LOCUS       DAZUMV010000047        16702 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000047 DAZUMV010000000
VERSION     DAZUMV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.047"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..599)
                     /locus_tag="ACG8FK_001428"
     CDS             complement(<1..599)
                     /locus_tag="ACG8FK_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5097042.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGE"
     gene            774..2198
                     /locus_tag="ACG8FK_001429"
     CDS             774..2198
                     /locus_tag="ACG8FK_001429"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5097043.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2497..3204
                     /locus_tag="ACG8FK_001430"
     CDS             2497..3204
                     /locus_tag="ACG8FK_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5097044.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3191..3514
                     /locus_tag="ACG8FK_001431"
     CDS             3191..3514
                     /locus_tag="ACG8FK_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5097045.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3603..4640)
                     /locus_tag="ACG8FK_001432"
     CDS             complement(3603..4640)
                     /locus_tag="ACG8FK_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5097046.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4961..5944)
                     /locus_tag="ACG8FK_001433"
     CDS             complement(4961..5944)
                     /locus_tag="ACG8FK_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS5097047.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6359..7129)
                     /locus_tag="ACG8FK_001434"
     CDS             complement(6359..7129)
                     /locus_tag="ACG8FK_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5097048.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7122..8057)
                     /locus_tag="ACG8FK_001435"
     CDS             complement(7122..8057)
                     /locus_tag="ACG8FK_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097049.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8373..10022)
                     /locus_tag="ACG8FK_001436"
     CDS             complement(8373..10022)
                     /locus_tag="ACG8FK_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5097050.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10139..12742)
                     /locus_tag="ACG8FK_001437"
     CDS             complement(10139..12742)
                     /locus_tag="ACG8FK_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5097051.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12754..13803)
                     /locus_tag="ACG8FK_001438"
     CDS             complement(12754..13803)
                     /locus_tag="ACG8FK_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5097052.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13829..14143)
                     /locus_tag="ACG8FK_001439"
     CDS             complement(13829..14143)
                     /locus_tag="ACG8FK_001439"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS5097053.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14159..14605)
                     /locus_tag="ACG8FK_001440"
     CDS             complement(14159..14605)
                     /locus_tag="ACG8FK_001440"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5097054.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14606..16528)
                     /locus_tag="ACG8FK_001441"
     CDS             complement(14606..16528)
                     /locus_tag="ACG8FK_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5097055.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         4948 a         3390 c         2748 g         5616 t
ORIGIN      
        1 aattcacctg gatctatttt taatattttt gcgtcaaatt cattcgcagt atctcctttg
       61 atacgtatgt gcgaagtctt tatttccaga cccagttcat ttgtgatata agcatataca
      121 gatttcttta aatgttttgg ttccaagcct ggtatgactg ctaatggcat gaatgtttgc
      181 tcaatcgagt atggttgaca atcaataaaa cggcttcgta caatattata tacatagtct
      241 tttacactga tacttaattt atcagctact tttttcgatg gaagttcaat tgagaaagta
      301 tggatttctg aatctacgtg ttcttcacca accacgctaa acaaaccttc gtttggacca
      361 agaactcgtg agttatcaat tggtgttctg acgacttcag tcccataccc acttctactt
      421 cgcaaaactc cttcggaaac aagtaagtct acgcctttct ttactgttaa cttgcttgta
      481 tgataaataa ctgccaattc atctcctgta ggaagtttct caccaatttt atactctcca
      541 tttacaattt ttttctttat atctaagtaa atttccatat acttcgcaat ttttttcaat
      601 ggtgaccctc caatcaaaaa catatcttca tctatacttt aacataaata aaccgataaa
      661 atccacatca aaattataca cacaataaaa aagatatata tatttaaaca aaactattta
      721 ctttaagttg taagcggtgt tatcattaag tggaaacagg aggtaaaaaa attatgacaa
      781 ctatgaaaaa gctaactttt ccagatgatt tttggtgggg ttccgcttgg tcagctgagc
      841 aagcagaagg aaaaggagaa acaggaaaag cagagactat ctgggatcgc tggtttaaag
      901 aacaacctta ccggttttat caacgaatcg attcctctat tacaaccgat cacatccatc
      961 aatataaaga agatatagaa ctaatgaaaa aaactgggca taactccttt cgcgtttcta
     1021 tttcttgggc acgaatgttt ccaaatggcg gtaccggaaa agtaaatccc aaagccattt
     1081 ctttctatcg aaaattattt tccgaaatga ataataacgg aatcaaagta tttgccaact
     1141 tatatcattt tgacatgcct gctactcttc aagataaagg aggatgggaa tcacgtgaag
     1201 tagttgatgc ttatgttcat tacgctgaaa cttgttttaa agaatttggt gacttagttt
     1261 gtcattggtt taccttcaat gaaccacttg ggcctatttt aggtacttat cttgaagatt
     1321 ttcattatcc aaacctcatt gactttaaac gcggagcgca agcagcattc ttcactatac
     1381 ttgcacacgc aaaagcaata caagtattta aagaactagc tcttcctagt aaaataggcg
     1441 ttattctaaa tttgagtcct acttttcctc gtagtcaaaa tcctgctgat gtgaaagcag
     1501 cagaaatcgc tgatttattt tatacgcgga gctttttaga tcctatggtt aaagggaatt
     1561 tttctaaaaa gcttatcctg ttattacaag aatataatca gcttcctgaa gattatacga
     1621 aagaagatct tgtatgtatc tacgaaaata ccgctcaaat tcttggacta aactattacg
     1681 agccacgtcg cgtcaaatct cgttcttcta tggtggatac atctagtcca tttttaccag
     1741 agtggttttt cgaaccacat atcatgcctg gacgacgaat gaacgaatac cggggatggg
     1801 aaatttacga aaaaggaatt tatgatttgt gcatggatat caagaaccat tatggaaaca
     1861 tcgaagcatt tatctcggag aacggaatgg gcgttgcgga tgaagaacgt ttcatggatg
     1921 atacagggca aataattgat gattatcgga ttgagtatat caaagatcat cttgcttact
     1981 tatggaaggc aataaatgaa ggatgtaaca ttaaggggta tcatctttgg acatttattg
     2041 attgctggtc atggatcaac gcctataaaa accgatatgg gctggtttca ctcgacctta
     2101 ctactcaaaa aagaacaatt aaaaaaagtg gcgaatttta caagcaatta agtgatacaa
     2161 atggatttag atacgatgta gacttgttag ttgaataata ttctaaaatt ccacttgaag
     2221 tgaacacatt agaaaacaag aataatgaga aataccaata acaaatactt tttcttcata
     2281 cttcatttat acttgtcagt ataagagact gtgacaaatg tagtgatcct cagaagttag
     2341 gtctggaaat ctaacttgag aagatttttt taaactacgt ccagtctttt ttatttgttt
     2401 aaaattgaag ttgacaagtc ttcattaaaa atttagaata ttcacaagtt tgacgacaat
     2461 tatgaaaatt tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt agatataaaa
     2521 actgcgatca aagacacgtt acctaccgtt tttggttata tcggtattgg acttgcattt
     2581 ggtatcgttg ggaaagctgc cgggtttcat ccgctggtcg tcacgttgat gtccctgctg
     2641 atctatgctg gttctgccca atttatcacg gtcagcatgc ttgctagcca tagtccactg
     2701 ctctccatcg ttctctcgac ctttctagtc aattcccgaa tgattctgat gagtatgacg
     2761 attgctcctt atttcaagaa aaatcggctg cttcaaaatc tgctgattgg cacattgctg
     2821 acagatgaaa gtttcgcact gggaatgaat aaattgaact atactggaca aaagttaaac
     2881 tttcgttgga tgaatacagc taattggatt tcttatttga cgtgggtggg ttcttctctt
     2941 gttggcgcac ttttagggaa ttttattact gatccgaaaa agtttggttt ggatttcgcc
     3001 atcgtcgcta tgtttatcgg gttactttat cttcaagtca tttctgatag aaacacctct
     3061 aaacgattac agttgatcct gattggtctc actttgattc tggtatatgt cggactgatt
     3121 tttatcccaa gtaacttagt gattttagtt gtaacactga ttgggtgcgg attgggggtg
     3181 tggatcaaac atgccttctt ttgagtttac attgctgact attttaggtt gtactttagc
     3241 aacttggctt tctaggatcc taccatttgt cttactgaaa aaattcgatt tgccgcaacc
     3301 gttgcttgag tatttaagtt ttgtcccgat cgtcattatg tctgctttat ggttcagcag
     3361 tctatttacc caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc
     3421 agttcctaca cttttagctg ctattctttc aaaaagctta ttggtgatcg tcttggtagg
     3481 aattctttcc ctttcgttga ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa
     3541 ttactgtcat gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt
     3601 gcttatccgt tgaacggacc actaacaacg acttttccta atatatgtcc tgtttctacg
     3661 tctttggttg cttgtttcaa gttcttcgca ttcatcccat ctgaatagac cttgctcaat
     3721 gtactgcgga taatcccttc atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc
     3781 gtttcgattt catagttata gtctgtttta gcgaacatat actcccagtc gaagctgata
     3841 gattggtttt tccaaacacc tatatctaac ggctctttga tccctacgat cgttcccaca
     3901 tgtccaaatg gtcggacaag gtgtttgatt tgatcaaagt agcgggtaat atcgaataat
     3961 acagcgacat actctacttg atcgattcct aattcacgaa aacttttact taagtcgtga
     4021 tgataatcga ttggatagtc tacacctgtt tttttcagcc attcaaaatt cttcggactt
     4081 gcagtcgcgt agacttctag gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg
     4141 cccccagctc cgttgatcac taagattttt ttcccgcggt tggcattttc ttctggagtc
     4201 agaccaaatt tttcaaataa caattcataa gcggtcaatg aggtcaacgg caaggctgcg
     4261 gcttcttcat ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc aaccagttgg
     4321 tattcttgat tgcttcctgc tcttgtcgtt gtacctgcgt aaaaaacgcg atcgccgaca
     4381 gaaaattttt gcacttcatc acctacagca acaactttcc cgactccatc aaaccctaag
     4441 atacgtaaag catttcttat cccgttatct tggcgaagtt tcgtatcgac aggattaaca
     4501 gatactgcgt tgacttttac cagaagatcc cgtgaactgg gtacaggtat ctgttctttt
     4561 tcatcaataa agctttttgg atcatcgatc gacaaccctt cataaaaacc gactaccttc
     4621 attaattcct tttctgacat acgaattcct ccttagcttc atcaagttgg agcatccata
     4681 cagttttcat ttcaatatat atagtatgct atttgttcca cttttattca tcataattat
     4741 tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac
     4801 ttttgtcaca acctctttta aacggtgttc aatcaactga ccctcggtat tcgctcaaaa
     4861 aacgacaaaa tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag
     4921 ctttttcacg acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg
     4981 ttggatgttc tttttcaaag gtgtctaaga tctctcgtac ttcatcagta gatttcgtat
     5041 tcatcaactg aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga
     5101 aacgatgtag tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta
     5161 gttgcagacg aagtagtccg atcagttctt tttctgtatg ttccctcggt tctttttcaa
     5221 atgcataagg attcttgaaa atcccccggc caatcatgat gccatctacg ccatattttt
     5281 cggccaattc ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg atcagtgttt
     5341 gcggagcgat ttggtcacgt aaagcaatga ttttagggat gacttcccaa tgtgcatcta
     5401 ctttactcat ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa
     5461 gtaggtgact gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga
     5521 cgctgacagg caaaccgcca gctttagccg cttcaatcaa ttgtgccgct acttctgggc
     5581 gaaggatcaa gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt
     5641 tgatatcgat tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt
     5701 ctggcttgtc tccccaaata tgggcaacca tcggctgttc atcttctgta aaggtcaatc
     5761 ttcctcgtac actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag
     5821 taaagaagac atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta
     5881 catcttccat tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag
     5941 tcatttattt gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca
     6001 ttgtcattat actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg
     6061 attgagcaaa aaaagactgt gacaaaaatc ttatcacaat tccttttccg gaataaatgg
     6121 tgttcaaaag cttgcatctg cggtaataag tccaaccaaa cgaaaaatag gaggagctat
     6181 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccactaa
     6241 acggtgttca atcagctgac cctcggtatt agctcaacaa acgacaaaat atgaggaact
     6301 attttcccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttttct
     6361 aacttctcaa tcctcttcct ttattgatca aataccaagt aacggcgtac aaaacaacga
     6421 tgaatagcac aagtacgatc atcgatgttg ctactggcac atcgattgtt ccaaggaatc
     6481 cgtaacggaa accgaagatc atataaacga tcggattgac tttcgaaaca gcttgccaaa
     6541 atggcggcaa catagagatg gcatagaaaa cgccacctag ataagttaac ggctgcaaga
     6601 caaatgtagg cacgatcgat acatcgtcat aagactgagc aaaaatcccg ttgagcagtc
     6661 ctgctaacga aaacaagatt gccgtcatca ataatgtaat gatgaccatg aaccatgaat
     6721 acacatgaag tggtacaaag aacaaggaaa tgatcgttac gagagagccg acgagaatac
     6781 tgcgacccac tccgccaatc acaaagcccc aaatgatcac atgcgtagga acaggcgcta
     6841 ccaagatttc ttcgatgttt ttctggaatt tttgagagaa gaaggacgaa gaaacgttgg
     6901 catacgaact agtgatagca gacatcataa tcaaaccagg cacgatgaat tccatatatg
     6961 aaaaaccacc catgtcaccg atccgaccac cgatcatttt tccgaaaata acaaagtaca
     7021 aggatgtcgt gatcacaggc ggaaccagtg tttggaccca gatacgcaaa tagcggttcg
     7081 tttccttgat agctaaactt tttaatgcag taaaataaag attaaacatt tttctcctcc
     7141 gtatgattat tttcttctgt gattttcaaa aacagttctt ctaaacgatt cgacttattc
     7201 ctcatagaaa gaactttgat tccttgtctt gtcagctgat cgaagattcc gttcacccct
     7261 tgatttcgtt caacttcgac agcgattgtc tgttcatctt caaatgtaca agcgtatcct
     7321 tcaatcacag gtttggtctc atatggcgct aaatcaaaga taaatgtttc aaattgcagt
     7381 ttggatagaa gctctttcat actagtattc tcgatcagtt cacctgattg gatgatcccg
     7441 atgttgcgac aaagcatttc agcttcttct aaataatgcg tcgtcaaaat gatcgttgta
     7501 ccattttcgt tcaattctcg gagaaactcc cacatctcgc gtctcaattc aatatcgact
     7561 cctgctgttg gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc cctagcaatc
     7621 atcagtcttc gcttcattcc cccggaaagc atacgtgcac ggacatttct cttttcccaa
     7681 agattggact gcttcaaata tttttcgctg cgtttcatcg cttctttgcg agagacaccg
     7741 tagtagcctg cttgattcac gacgatttgc tggaccgttt caaagggatt gaaattgaac
     7801 tcttgcggga ctaatccaat ctgttgcttt gctcggacta gatccgtatc cagatcataa
     7861 ccgaagacat tcacttttcc agatgtttta ttgactaaag aagtaatgat tccgatcgtt
     7921 gtagatttcc cagctccatt tggtcctaat agcgcataga aatctccttc tttcacggaa
     7981 agatcgattc ctcgcaatgc ttcgacccca cttccataaa cctttttcaa attatttatt
     8041 tcaagcgcat ttgccattta tttcctatct ccttatagac atgattcttt attgtatcat
     8101 ggaatacagc agatgaaaaa acaacttggt tccagcgaaa aatcaggttg ctgggctcag
     8161 gaaaatctcc taatgaaaga catcaatcgg acgaagtcag acaacctttt cagaaaaaac
     8221 aaatcgaaga taaaggatta ctattcatat gtgcaacaaa aggagcgtga gacaaaagta
     8281 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaaa agccaagatt
     8341 tttgatccaa taaatacttt cgatcacttc gcctatccca atacatgcca ctgccgcttt
     8401 tcatcaaata acgcggcttc cgtttttgcg gtttctacta aagtctctgg gtaatccaaa
     8461 actttcagca aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa tttgaaatca
     8521 aaactgattc cttttccttc tgttacttgt tcttcaaagt ggacattctc acaataattc
     8581 tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga tggatactcc
     8641 gcaagccacc gaacgactga agcggaagaa gcaatccgtt cgatcgtatt tgtccctttt
     8701 aaaatctcat ctacgaagca gtagcagcgt tctttggaag ccacttggtc caacaaacgt
     8761 ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt caaataaatc atcttcgatt
     8821 gccatggaag tcaacacatg tccaggttgc agggtaaact gttctgcaat cgctgtttgg
     8881 atcgtctgtg ctagaataca gctgatggct atacttttga catacgtaga ttttcctgat
     8941 gcattggaac ccgtgactaa tgtattcttc tgccagttga ccggattcac taccgcttct
     9001 tttaacaatg gatgatagat atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg
     9061 caagtgatgg gcatataagt acggtaattc aatattgcag cggctacttc taattctccc
     9121 agcaattccc acagcttgat ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa
     9181 tgataggaga taaatggcag catgaacatg atgttgacat attcaaaaat caactctgct
     9241 tcactcccac tcttcatacg aaatgaaaaa cctaatttag ggatgtctct taatggttta
     9301 gtttgctgtt tcagctcttc ttgaagtggt gtcggcagtt ttgctagctg attggcggct
     9361 gaaatcgttg aaaccaaata actcatgcta ttcagctctg tttctagtgt ttgtttcttc
     9421 actagataat agaccgtatt gaatactaac ccaccgattg ccaaatagat tcctagtact
     9481 tgtcctaaaa gaagcaaacc caaacccacc agcggaagca agccaagaaa aataaataaa
     9541 cctacgcgtc caatcttttt actttttcca tccgctagat attgttttgc aaaattatga
     9601 tctttttttc ctaaacgagc gaattgatat tggatttttt ctctcagttg aggattttcc
     9661 tgataaaaag caatcagttt ttctaactgc tgatcttcac caaaatcaaa gctgcgtaaa
     9721 cgttgataca acgcttctga acctacacta gaataggtac tgttgattcc ttcgaatact
     9781 tcgaacatat ccaggtcata ccaagtaaga tcatcgattt cgctatccca tgaacggaac
     9841 ttcttctccg tgagccatgc ttccttcaag ctttcttctt tatcaaatct cggttgataa
     9901 ggaatttttt cccatttgct acgaactgtt tcctttaatt tcaaacgtga aaaaatgtct
     9961 aaagcaacta ataaaataac agctgcaata ataattaaaa cgataaattc tctttgaccc
    10021 atatccctct ccgttccttt atttgttagc tttagtatag cactgttgcg ctatttgaaa
    10081 agagggtaaa aaaacacctt tcaaaataaa aggtgttttt ttcttgtata ttacatattt
    10141 atctctcatt tatcaaaatt gacaaataat catagggagg aattgttgtt aattcttgtt
    10201 tattctccag tgcattgaca actgtcccaa gggaaaggat agcctcaggt actatttttt
    10261 cttgttctgt tggattagct aagcgaagaa caaatccttg ttcatgaaaa taagaaggat
    10321 aaatcgatga tacaattaag tcttcaggta attcaatcaa tgaaaattta cgaggaacat
    10381 caagttcatt ttgtaatggc caaatcttgt tatctaacct gtgaataaat acattcagtt
    10441 tctgcttttg ataagaaata gattgttcta accgtttttc tgcctgtttt gccactttga
    10501 actcattaaa tcttccttct tctacattaa atccaaaagt gactttatac tcacctatct
    10561 cttgagctag cggtgtttcc atcatgacat gcccttcatt tgtcgtatct cctgaagctc
    10621 gcccaggtcg ccaagcaaga ttaggttttc caagttcccc tgttgttgaa aataaggtta
    10681 tgattaattg attttctata cgttcatact cttttaaacc atctgcataa aacgtaaaat
    10741 aatgatcatt ttctgccaca gagacactct tatcgaatat ttctaaatta acaggtttct
    10801 ctaccatagt ttctgaccaa ttcggaggaa tgacatcagg ggtattttca ataaatccgt
    10861 tttgaatttg tgcaattgat ttttcagatt tatctaaaac attaattgct aagcgcatac
    10921 gatgcgagta aacagtatta tgaattttga gtgtgccttc aacgatttga ctttctccca
    10981 gactgaacga tatttcataa gatatttctt ccatttcggg tttttcagat aaacgatctt
    11041 ttaaatcttt aggaagttga gcttttccgt aaacaactaa tgtttcttgt aaagaatctt
    11101 tatatacttt agctgtttct aattttagag atagttctgt atctccttct aatggtgaat
    11161 aatcatacgt atcgccatca tttgctgaat cgatcaaatg aacgaaatca agatattgtc
    11221 tatctccaac tatcaaattc aattgcccat ctttaaaaga aagtttatat ttattgttac
    11281 ttatctcagt tttgtctatc tcgattgctg tttctaattt ttcattattt ttctcaaatt
    11341 caacaacagt gtatcctagt gctggcacat ttacatttat ttttatgtca agttcataat
    11401 aagctggttc atcaaaatat tcgaaacctg tagcaatcat ccgataagca tttttccgtt
    11461 ctggaaaata aacttctttt tcaatgatat tatagggact acctttgaag tgtattttct
    11521 ttgaatcagc tactacatgc acgattttcg atccactata attctcagtc gttgtgttga
    11581 aaacaaggac ttcattcgat tttagcttta atgcatcagc catccgtttt acaataaggt
    11641 tttcgattcc gtcagctatc tcattagctt ctttgattcg atggaaaata tcttccgcaa
    11701 cattatctga aacacagcca ccaatactat catgagcctg gttttgcaaa atctttttcc
    11761 ataaccgacg aagtaggcct ttactgatat ggataccatt tctttctgca atcagcatga
    11821 gcggctcaac tcgttttaga attttttgtt ccaatttaaa attttctatt ttcatttttg
    11881 ttcttactga gccaatagaa cgatgaacac gagagtaaac cggctgacga agttcacctt
    11941 tatattcagg taattctttt ttgtttctga taacttctac ataagaagga taatctgaaa
    12001 taatattgtg gaatttactg tatttattca acttctctac gacttgatca aaatttaaaa
    12061 tcattgactt ttgatctaca cccgaaggta ctaaaatatc tttgtttttt ccttgcatat
    12121 gaagcatttc aactgcttca tccaactttt cttttgcaaa ttcaggaata tctttaattg
    12181 cgtctaaggt catcattcca gtcatataac caaatggaaa attcatggca taaacaaatt
    12241 tatctcccaa tcctttccag ataaaataag gagatggaac catcgtttca aagtttaatc
    12301 ctcgccaaca catgaaatta tcgatttcta cttgatgcaa aagtgtaggc aaattcgcat
    12361 tgaacccaaa cgtgtcaggc aaatacccta ccatcattgg atctccatat ttaaggatcg
    12421 tatcactgat tccaatcatt aagtttctta aaattgattc agcatccact aataaggcat
    12481 ctgtttgtgc ataccacggt cccacgaata gttgtcctgt tttcatcagt cgttggataa
    12541 ccttaagctt ttgcggattg atctccaaat actcatcgac gattgaagtt tgtccatcta
    12601 aacaaaagtt tactttaggg ttcctttcca attcatctag aacttcagta aagacctgat
    12661 cagacaatac gatggcatct tcatttgtaa aataccattc tctgtcccag tgcgtgtgtt
    12721 gaattatatg tgcatttacc atatcagatt ctcctacttt ccttcttcta ctgaatttac
    12781 ttgttgttct tttttatact tgatggattt tagtgcattg accattaacg ctgtaaccac
    12841 tcccccaata acaattgata ctaaatataa aggtatattc gtaacaaaag gaattgccat
    12901 aaaggttgaa agcattgttt cttgagtaac accaaaaaat ccatttagac cacatgctac
    12961 tgcagtgccc accacgatgc ttggtattac tcgtaaagga tctttcacgg caaacgggat
    13021 cgtcccttca gtaatcccca caaggcccat aatcatagct gtctttgcat tatctcgttc
    13081 cgattcatca taaagttttt tgtttaaaat tgttgctaaa aacattccaa atggcggaat
    13141 agcaattcct aataacatgg ctgtactagg tccaaagctt ccgcttgaaa aagaagcaac
    13201 cccaaaagca tatgcgattt tattgatagg tcctcccata tcaaacgcca tcattcctcc
    13261 taaaatagcc gacatcaaag cagcatttgc tcctgacatg ccttctagcc aattagtcag
    13321 tcctgttgtc attgcttgaa taggcgtagc tagtacatac cataatatga ttccaacaac
    13381 taatgtagta acaaaaggaa tcagcataat tgattttaga ctaataactt gcatcggtac
    13441 ttttactttt ttcaatgcat tagctaaata gccacaagct aatccagcaa ccattcctcc
    13501 aataaatccc ataccaagat tttgacacat catcccagtg ataaaagctg gtgctagacc
    13561 gggacggtca gcaattgaat atgcgatata ggctgaaatc atcggtacga taaactgtaa
    13621 tcccgtctga ccaatcattc gcaggactcc ccagaatgta tccgtctggt cccaaacacc
    13681 tgtgccacca aaaatgactg caatagaagt tagtaaccct gctgctacga caagcgggat
    13741 catataggaa accccacttt ggaaatgatt tagaatttct cttccttgtt tcttgaattc
    13801 cattagtaac tcccccacga tatatttttt atttgttaat taatgcaatt gcttcatcaa
    13861 tcgtctcttc accatctttt actacctctg aggtccctac tgttaaaatc ggtttaccgt
    13921 caaagcgacc tcgttctgaa acatttgtat ctactgcaaa tatgacaaca tctgcctcgg
    13981 aaacatccga aattgaaagt ttgttttcta tccccatagc cccctgtgtt tctactttaa
    14041 tatctaaccc tttcttcttc gcaaactttt ctaaattagc ttttgccata taagtatggg
    14101 caatccctgc gatacatgct gttactgcta ctatttttgt catatgaaat tcctcctttt
    14161 aaaatgatag agcttttacc aattcttttt tatttctcga atcttttatt cttttaacaa
    14221 actcgtcatc catcaatctt ccagataatt ctgctaaaat acgtaaatga ttagtacctt
    14281 tatcagtgtc ttttattgct aacaaaaaga cgagatcaac tggttgatca tctaatgcgc
    14341 cccattcaac agaccgcttt aacttggcta cagccactgt tgattcaatt actgctaggc
    14401 ttttgccatg aggaatggcg atatttttcc caattcctgt ggtcatatgg ttttctctgt
    14461 caaatacgtc tttttgaaat tgtttgtggt cagacacaaa cccattagtc tctaacaatt
    14521 tcgataattt ttcgactaca ctttttttat cagatgcatt taaatctatc actatattat
    14581 ttatattgat tttttcactg atcatttata agccttcttt caatatataa actaattttt
    14641 caaacgacga actcgttttt aaatttgcta ataactcatg attcgaagcg atttcattga
    14701 atgtttcata aattttattc aattcaggtt ttactttttc attaagtgca aaaaagaaaa
    14761 cagaatgaac aagtgtccca tcccaatcaa ttccttctgg attaacgtag acacagatta
    14821 ctggcttttt tataaaatct acttcgatat gcggcatagc aaaatcattc atcgctgttg
    14881 aagatagttt ttctcttttg attgctccct ctagtatccc ttctttcgcg taattttctt
    14941 ttaccaattt tttggtaatt atatttaaaa cgttcacttg attatccttt atttggtggg
    15001 taaaaaagca aaatcgttta tctaataaat ttagaaaagc atcttctgtt tgttcattta
    15061 actgtagata ctgttttact tttctgacat ctgcccctaa catcaaagga ctaacaacaa
    15121 taacaggaaa atttgtattt tcaatcggaa tcgtgcttat aacaagttca tcagtgactt
    15181 ttctatcttg tagttcattg atgcttagta catcttctat ctcaaccata tctgcaaaac
    15241 cgttatttat tctttgctct aataattttg atgttccata tccgctacta caaactaata
    15301 tcactttcgt tttgtcaggt ttgtaacgat ccaaaagtga ttgaatatgg agtgttacgt
    15361 aagcgatttc atcttcatta aaatgtatac agaatctctc ttcgatttcc tctaataaat
    15421 ctacacttat cataaatgac cttttaaaag aatatttgat tttgtccgta taagggttat
    15481 agatatttac tcccaatttg agtcttttaa ctgctgcatt gagatgtaat actagattat
    15541 tgatcaattc ttcatcagga taaaattcat ttaatttaat tcttactaac tcttgtagct
    15601 caacacccat atctaaatag gaaagcttcg tttgatctaa gtcgtgattc atagcatttt
    15661 tttcaatcga agtaatgtga atgtcaacat aagcttgttc atctattggc acaataattg
    15721 aaaatgcttt ttcaatcatt ttgattaaaa taagtgtatt ctttgagatt tcttcttcag
    15781 aattattact acgtgataag aaattgcttg tttcaatcct ttctatcgca atagcaaggt
    15841 ggatagctaa agtttgaaat tcataatctg taaatttcaa ttgtgtctta ttcatgtatc
    15901 gattaagcaa ttcggctact tttttcaata tgtccttatt taagaaatta ttcgcttcac
    15961 caatgaaatt gatattcaag tctaagagat ctgttttact gttagatgct gtaaagccac
    16021 cccaataata gttcacaact tctgacatca accttctttt acttttttca tcagctttga
    16081 tgcgcacgcc atttcgatct gaaactaagg acatcccatg cttgtttagt ctttttctaa
    16141 catctttcat tgtgctttct accgtcgacc tgctcacgta taattcgtca gcaagatttt
    16201 gaatctttag agaatcatta gtattcaata atttagcaaa cacataagtt agacggtcct
    16261 ccggagagtc tgcgatattg attttatgac tggataataa atttcgaagt gtttcttttt
    16321 ttccaataag cgaaacgcct tttttaggtt gaaccaacat ttgtacatct ggcatttttt
    16381 ggattatttc tttcaaaatt aaaatatctt tgctaattgt tttcgtagat acatataatt
    16441 gttctgataa ttctttgtaa gttgtgtgct cattttcaat caaaaagtat agtattttca
    16501 tttgccgttc gctaaattcg atgcccatta tcttcacctc ctgaatacag cttaagtgta
    16561 caattattat ctctattttc atacagaaga aagttcttga ttaatgtaga agtttctaat
    16621 attcttgatt atttcaaatc aataaaaaag cctgcaatat ttatatcaca ggcttttttc
    16681 tctattaaat atatataaga ta
//
LOCUS       DAZUMV010000048        16695 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000048 DAZUMV010000000
VERSION     DAZUMV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.048"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8FK_001442"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8FK_001442"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS5097056.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACG8FK_001443"
     CDS             complement(1173..1868)
                     /locus_tag="ACG8FK_001443"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS5097057.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACG8FK_001444"
     CDS             complement(1881..2597)
                     /locus_tag="ACG8FK_001444"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS5097058.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACG8FK_001445"
     CDS             2927..3532
                     /locus_tag="ACG8FK_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS5097059.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACG8FK_001446"
     CDS             3857..4180
                     /locus_tag="ACG8FK_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS5097060.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACG8FK_001447"
     CDS             4362..5732
                     /locus_tag="ACG8FK_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS5097061.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACG8FK_001448"
     CDS             complement(5862..7163)
                     /locus_tag="ACG8FK_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5097062.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACG8FK_001449"
     CDS             complement(7393..9384)
                     /locus_tag="ACG8FK_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS5097063.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACG8FK_001450"
     CDS             complement(9528..9794)
                     /locus_tag="ACG8FK_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5097064.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG8FK_001451"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG8FK_001451"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS5097065.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG8FK_001452"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG8FK_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS5097066.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACG8FK_001453"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACG8FK_001453"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5097067.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACG8FK_001454"
     CDS             complement(13954..14475)
                     /locus_tag="ACG8FK_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS5097068.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACG8FK_001455"
     CDS             complement(14478..15320)
                     /locus_tag="ACG8FK_001455"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS5097069.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGRVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACG8FK_001456"
     CDS             complement(15313..16038)
                     /locus_tag="ACG8FK_001456"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS5097070.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACG8FK_001457"
     CDS             16214..16510
                     /locus_tag="ACG8FK_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097071.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
BASE COUNT         4692 a         3477 c         3084 g         5442 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcttccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca acccgtggtg
    16681 ctagttaatt ttttc
//
LOCUS       DAZUMV010000049        16509 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000049 DAZUMV010000000
VERSION     DAZUMV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.049"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..387
                     /locus_tag="ACG8FK_001458"
     CDS             <1..387
                     /locus_tag="ACG8FK_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFS5097072.1"
                     /translation="QSEQYLERNLAMLKLANHVGKGWFAIELGEKIDFLIKIPKYILN
                     AIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTADLNIAIISLLEYF
                     GKDEDVVYLINQIENYTKILYAEVSK"
     gene            384..2177
                     /locus_tag="ACG8FK_001459"
     CDS             384..2177
                     /locus_tag="ACG8FK_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5097073.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            2377..2691
                     /locus_tag="ACG8FK_001460"
     CDS             2377..2691
                     /locus_tag="ACG8FK_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5097074.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            2712..3089
                     /locus_tag="ACG8FK_001461"
     CDS             2712..3089
                     /locus_tag="ACG8FK_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5097075.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            3099..3872
                     /locus_tag="ACG8FK_001462"
     CDS             3099..3872
                     /locus_tag="ACG8FK_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097076.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            3894..5297
                     /locus_tag="ACG8FK_001463"
     CDS             3894..5297
                     /locus_tag="ACG8FK_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5097077.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            5479..6663
                     /gene="mobT"
                     /locus_tag="ACG8FK_001464"
     CDS             5479..6663
                     /gene="mobT"
                     /locus_tag="ACG8FK_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5097078.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            6660..6950
                     /locus_tag="ACG8FK_001465"
     CDS             6660..6950
                     /locus_tag="ACG8FK_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097079.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            6947..7168
                     /locus_tag="ACG8FK_001466"
     CDS             6947..7168
                     /locus_tag="ACG8FK_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097080.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            7210..7989
                     /locus_tag="ACG8FK_001467"
     CDS             7210..7989
                     /locus_tag="ACG8FK_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS5097081.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            8050..8550
                     /locus_tag="ACG8FK_001468"
     CDS             8050..8550
                     /locus_tag="ACG8FK_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5097082.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            8606..9244
                     /locus_tag="ACG8FK_001469"
     CDS             8606..9244
                     /locus_tag="ACG8FK_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFS5097083.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            9316..9711
                     /locus_tag="ACG8FK_001470"
     CDS             9316..9711
                     /locus_tag="ACG8FK_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5097084.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            9695..12148
                     /locus_tag="ACG8FK_001471"
     CDS             9695..12148
                     /locus_tag="ACG8FK_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5097085.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            12145..14172
                     /locus_tag="ACG8FK_001472"
     CDS             12145..14172
                     /locus_tag="ACG8FK_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5097086.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            14169..15146
                     /locus_tag="ACG8FK_001473"
     CDS             14169..15146
                     /locus_tag="ACG8FK_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS5097087.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYELAEQFSKEKSGGQKAD
                     YPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETVQVIMDEALKYEGFPY
                     VFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEISMEEAQAGDLIFFHST
                     YNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGARRVIHN"
     gene            15163..16092
                     /locus_tag="ACG8FK_001474"
     CDS             15163..16092
                     /locus_tag="ACG8FK_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5097088.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            16406..16453
                     /locus_tag="ACG8FK_001475"
                     /pseudo
     CDS             16406..16453
                     /locus_tag="ACG8FK_001475"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5667 a         2688 c         3220 g         4934 t
ORIGIN      
        1 caaagtgaac aatatttaga aagaaattta gcgatgttaa aactagccaa tcatgttggt
       61 aaggggtggt ttgcaattga gttaggtgaa aaaattgact ttttaatcaa aattcctaag
      121 tatattctta atgctattgc ttttgcatgt caggaaatag tagatctctc aatttataaa
      181 aaaattctac tccatacaat cgacttgtac gaggaaacta cagaattgtc agctttatca
      241 gctagtttaa aaacaagtga tacagctgat ttgaatatag caataatttc tttattagag
      301 tactttggta aagatgagga tgttgtgtat ctaatcaatc agatagaaaa ttacacaaaa
      361 atactttatg ctgaggtttc aaaatgacaa atatcttcga aaaaatcgta aatgatttaa
      421 atttagaaca aaaagatgtt gtggaaagtg aagataacct ttatgtaaat gcatgtcctg
      481 ggagtgggaa aacaagggta cttacaagga aaattgctta ccaagcaatt aaacacgatg
      541 gatcactaaa aagaataatt gctatcacat acactaatag agctgctgaa gaaattaagg
      601 aacgcctaag tttacttagt atagatgatg atattaatat ttgggtgggg acaatacatc
      661 agttttgtct tgaatttatt atttatcctt ttaaaatgaa tctctcaaga gtatcaaaag
      721 gatttacaat tattgatgat tatactcaaa gagcttacgt cagccaaatt aataatatgc
      781 tgaatttaaa tctcaaaacg tatgaactga acaaaattga tttgtcactt tctcatgatt
      841 ttaaaattaa tgaagttcag tatactcaag tttctgaaat gtatcacact atgttggaaa
      901 aaaataaaga aattgatttt gatatgattt tactagtagc gtataaaatt ttaactgtaa
      961 acagtaacgt tgcacgtaat attgctagta atataagggc gatttatgtg gatgaatttc
     1021 aagatactag agaattacaa tacaatgtaa tagctaaatt acttcaaaac aaccctcaaa
     1081 ttcagtcaat gtttgttgga gatattgacc aagcaattta tggatcatta gatggtatag
     1141 ctaaatcagt tgaagaacta gaaatgctaa ctagtcacac ttttcaatca aagacgctac
     1201 atggctgtta caggtctaat cagagattag tagacttcta ctctaatttt cagagttgtc
     1261 cttacgagat tgaaagcaga gggaataata aaaacgaccg tggttttata agttatgatt
     1321 gcaaagttac taaggaggat ttacccaaca taataaaaaa tataataaag gaaaagattg
     1381 aaagcggcat tcatgaaaaa gatatttgtg tacttgctcc tcaacactat cctctgtttt
     1441 cactgggtga gtatttaaag aaagaattac cgtattgcaa ttttgatgct ataaatatat
     1501 caccaattaa ggtaaataat catagtttat ttttcaagtt agcaattttg tactttactg
     1561 aatcaggtga aaaggttcga agaagaaagc taattgcaaa cgatatttta attatattaa
     1621 aaaatgaatt tggaatagaa gttaaagatt attttattga cttagattta ttacagctaa
     1681 taaacagtgt gaaaagaatg tatagtggag acgacaacgg tgttgattta tacaaatttg
     1741 ttaccaaaca tatatttgtt aatcttaata ttgacgagaa aaattacccg tctattttta
     1801 agcactatct attttttatt aacgaagtag aagatcggat aaaaaataat tcattatcta
     1861 gtagtgtatc tagttttaag aagatgttta aatctcgaga tggtatcaca cttactacag
     1921 cacataaagt taaaggaaat gagtatgata ctgttatcgc tattaatctt ctaaacggga
     1981 aaattcctca ttggaatgaa atttttaatg tttctgataa aggaattagt tcagcgagaa
     2041 aactactata cgtaatttgt tcaagagcaa aaaataattt atttcttttc tcagaggtgg
     2101 gttattttac gagtaaagga gacttgcttt atcccactca attattagat aaaatagaat
     2161 ttgattataa tatttaaaat acctaggtag tcgttgtttt aaatatagta ggaatcggac
     2221 gctcagtacg gagcgtctat ttttttaccc aattttgaaa ggaagtgaac ttatgaaaat
     2281 aaaaaatcaa gaatcaaaag gtcgctctcc cccctttaaa accatcacac aaataagatt
     2341 cattcaaaca taaaaacaga aaggataggt aaaaatatgg aacttaaata tgtcattccc
     2401 aatatggaaa aaacatttgg acaattagaa tatgctggtg aagggaacat tgaacaacgt
     2461 cgaataaatg gcagaccaac cattctctct cgtagttaca atttatactc aacgattcaa
     2521 cgagcagatg atattgttgt cattcttccc tccgaagctg gcgagaaaca ttttgaatcc
     2581 gacgaaaaag tcaaactcat caacccacat attaccgcag aaggttataa aattggcaat
     2641 cgaggattta ccaattacat tatgcacgca gacgatatgg tgaaagcata agaaaggaaa
     2701 ggtgaaaaaa tatgagatta gcagaaggta ttgtgattga aaaagaccag acatttggca
     2761 cattgaaatt ttccgccttg cgtcgtgagg ttcgtatcca gaatgaagat ggtacggttt
     2821 ccgaagaaat taaagaacgt acctatgact taaaatcaaa aggacaagga cgcatgatac
     2881 aagtcagtat ccccgcttct gttcctttga aagaatttga ctacaacgca gaagtggaat
     2941 taatcaatcc tgtggcagat accgtagcga cggctacttt tcgaggggca gacgttgatt
     3001 ggtacgtgaa agcagacgat cttgtattaa aaggacagac aaaacctcat cagaaatata
     3061 aaaaagaagc gtccgaaagt gaaaaatagg tgttgcttat gacaaacaca ccattgattt
     3121 tgaaagaaat tccgaaggat gaagccattt cttttattcg gcaataccat tattcaaaga
     3181 tactccctag attgtgtaaa tactttctag ggattttttc agaagaaaaa ttattaggcg
     3241 ttgtggaatt aggctggggt acgcaacccc tacaaaccat acgcaaactc tttcccgaca
     3301 gttcccttca aaccacagat tatttagaaa ttggaaagat gtgttttctt ccagaaatga
     3361 atcaaaccaa ttattttggc agtcaagctc tttcagctct aattaagtgg ttgaaagaac
     3421 atacggactg tcatttttta tatacactgg cagatggaat tgagggaaaa tgtggctatg
     3481 tctatcaagc gtccaacttc ttttactgcg ggtatttcaa gacaagtgtc tatcgtgata
     3541 agcagtcatg ggaaaagata caccctcgaa gtgctcgcct tttattagag gaaaatgctc
     3601 gctttgaaca ggtagagaaa aagcattggc tttcccaggc attttgcgaa tacaaaggga
     3661 ttgagaaaat caatggacgc atgtttcgct atctctatcc gttgacaaaa gaagcgaaaa
     3721 aactgttagg acacactctc tatcgacgtc attattatcc gaaagaaaaa aatttacgct
     3781 ttgaaaagcg aatcgcttat cagaaatatg aagccatttc tcaaccaact tttgataaac
     3841 aggctcgtat ctacaataca caattatttt gataggtgga aaggaggatt tttatgccat
     3901 tgcttcatga cagaggagag cgaatcaaag ccaacgataa gcacttggtc tatcattttg
     3961 tactaatttc attgctggta gtttttctag tcgtcctact cccttttcat tggagagcgt
     4021 tacaatcggt gaactggcaa gcagttcagt tagatacatt tttctatcaa cttcatacgc
     4081 catacttttg gagcagtatt ctcactgctc tttttgtatg cggacttatc agctggcttt
     4141 attatcacta tcgtatagat tctataaaac agttagagca ccgtcaaaag ttagctcgta
     4201 tgattttaga aaatcattgg tatgaagccg aagaaactca atcagaggga ttctttaaag
     4261 atttacccag caagaaaacc aaacaacgaa tccgccattt tcctaaaata tattatcgaa
     4321 tgaaagatgg actaatccat attcatgtag aaatcacctt agggaaatat caagatcagc
     4381 ttttgcacct agaaaacaaa ctagaaacag gattgtattg tgaattagtg gaaaaaacgt
     4441 tgaaagattc ttatgtagag tatgtcctac tttatgatac tattggcaat cgaattggca
     4501 ttgaagacgt ggtagctcaa gatggacgtg tcaaattgat ggaatcggtc tattgggagt
     4561 tcgattctct ccctcacatg ttaattgctg ggggcactgg tagtggaaaa tcctatttta
     4621 ttctcacatt gattgaatct ctcctgcata caaatgcaaa gctttatatt cttgacccta
     4681 aaaatgctga tttggcagac ttaggggcgg tcatgaataa tgtctattac cgtaaagaag
     4741 atatgttggc atgtatcaat caattttacg aagatatgat tgatcgcagt gaaaccatga
     4801 aacaacaccc taactataaa acaggagaaa attatgctta tcttggtttg tctgccaatt
     4861 ttcttatttt tgatgaatat gtggcaatga tggatatgct aggtcgtgaa agtaccaccg
     4921 ttattcataa actcaaacaa attgtaatgt taggacgaca agcaggcttc tttttaattc
     4981 ttgcctgtca acgtccagac gcaaaatatt ttagtgatgg gattcgggac aactttaact
     5041 tccgtgtggc tttgggtcgt atgtctgaaa tgggctttgg tatgatgttc ggaagcgata
     5101 cacaaaaaca atttttctta aaacccatca agggaagagg ttacgtggat acaggaaaaa
     5161 gtgtgattag tgagttttat acaccccttg taccaaaaag gtacgatttc ttaggagaaa
     5221 taggaaaagt cattcaaaaa aagcaatctg agcctgtacc gcacgaagtg aaaggtacag
     5281 gttcagactt gctttgattg gtgtggcgtt agccacgcca atcctttagc cccctctatc
     5341 taacaggggg gcacaaatac aactgaaaac aggcaattct catcaaccaa tgttgatggt
     5401 acaaaggatt gcggaaaata tgaaaggtgt aaactcaagg gtcatagttt acatcttttt
     5461 tagattggag gtttgcgaat ggaaaacgaa aaaatttgga ttaaggaatt gaaagaaaag
     5521 cgactggctt atggcgtttc tcaaaataaa ctggcggttg cttctcatat taccagacct
     5581 tacctatcgg atattgaaac aggcaaggca gtccctactc aacaggtgaa agaagattta
     5641 ctgaacgctt tggaacgctt caatcccgat aacccattgg agatgttatt cgactatgta
     5701 cggattcgat tccctaccaa tgatgtggct caaattatcg gtgaggtttt acgtttgaat
     5761 atggattata tgctacacga agactttggc tattattctt atcccgaaca ctatcgtttc
     5821 ggtgatattg ttgtcctagt ctctcatgat gtaagtaaag gtgttttatt agaactgaaa
     5881 ggaaaaggct gtcggcaatt tgaaaacttt ctattggctc aacatcggag ctggtatgat
     5941 ttctttcaag attgcatgga acataaaggt attttcaaac gcttggattt agctatcaac
     6001 gataaaacag ggattttgaa tattccagat ttggcaaaga aatgtaaaca ggaagaatgc
     6061 atttccgtct ttcgaagctt taaaaattat cgcagtggtg aattggttca ccgtgatgaa
     6121 aaaccataca tgggaaacac actttatatt ggctcattaa aaagtgaagt ctatttctgt
     6181 atatatgaga aagattatga gcaatacgtg aaaaatgatg ttcccttaga agacgcagaa
     6241 gtaaagaatc ggtttgaaat tcggttaaaa aatgaccgag ccacccaagc catgaaagat
     6301 ttattggcac atcaacaggc ggaaaaaaca gcctttgaaa ttatcaatcg ctatatccgc
     6361 tttgcagata aagacgacac taaacgtcgg agtgattgga aaactaatga acgttgggaa
     6421 tggtttatcg gtaaaaatcg tggagcatta cgcctaacca cacagccaga gccttattct
     6481 tttgaacgaa cattgaactg gttacatcat caagtcgccc ctacattaaa aatcgcaagc
     6541 attttagatg ttctcaacgg tacaaccatt atctccacca tgattcagga agccaaactg
     6601 acagaaaaac atgagaaatt aattgaacaa caacatctag ccatggagga tttgattaca
     6661 tgagcctttt aacaaaagaa ttaaagaagt tagggtttca gtgtggcatt gagtttcaag
     6721 cgtatataca aaatacaggg aaatatacgt ctctcattat tgagggaaaa cggcaagctg
     6781 gtgataccat ctatacctat gatttttaca aggtgagatt ttataataat tataccaatc
     6841 gagtaaccgt ctatggagag catttaaccc cttttcaatt attaaggcgt gtaaaaagct
     6901 atatctatta tcgagaaaaa tatcttaagg aaaggaggac aatcacatga attttgggca
     6961 gaatttgtac aactggttct tggataatgc acagtcattg gtattgttag cgattgttgt
     7021 cattggactg tttttaggtt ttaaacgaga attttcaaaa ctcattggct tcttgattat
     7081 tgctctaatc gccgtcggac ttgtgttcaa tgcagctggc gtgaaagatg tgttattaaa
     7141 cttatttaac cgaatcattg gtgcttaaca gatatttttc atgataaaat aggaagtata
     7201 tattttgtta tgaaaggatt tatgaatatg tttacaaatg atcaatttaa atctctttta
     7261 attaaaacat tagaaagaaa gagctcttca aattattatg aaggaggtaa cgagattgtt
     7321 tctaaaatga aaatatcaga ggtaacttta gatgagactg acgattttac ttattataaa
     7381 ggatataaaa agggctggaa tacgctttgc acatatctta aaataagagt tccttttgat
     7441 gatttagact tttttgaaag tcataaagat ttgattaccc agacagcaag ctccatatat
     7501 gataaacaag gtgataatgt attagtggat actattttag taccacttcc agagaattac
     7561 gaagtaatta actttagtca gttgaaaatt agtgatgtgg tttcacaagc tattgaagac
     7621 gctgagtctt ttatgagtaa tggtgaatat caacgtgcct ttgacagagt tcacactgct
     7681 tttcatggat atttaattga aatactaaaa aaatatgaaa ttacagttcc tagagacgaa
     7741 aatctatcta aattatatag taggatacaa caattaattg aaaaagaaat tcaacctact
     7801 gaacttgctg atatagtgaa gacaacgata aggtcatcaa atggaatgat tagctctctt
     7861 aatgaagcaa gaaataggca ttcacttgca caccctaata caaatattat tggaaagaga
     7921 gaagcaaaac taataatagg tatttcaagt acagttacag attatattag cggatacctt
     7981 gataagtagc gtcattaact gattgctact ttttttatga ctatttttat aaaaaggatg
     8041 tgataagaat tggaaatgaa agtctatgtg gcaaatttag gacggtataa tgaaggtgaa
     8101 ttagtcggag catggtttac tccaccaatt gatgaggaag aaatggcaga aagaatcggt
     8161 ttaaatgagg actacgaaga atatgcaata catgattttg agcttccttt tgatgtggac
     8221 gaatacacgc cgattagtga aatcaaccgt ctatgtgaag ccattcaaga aatcgaaggt
     8281 acacctattt ataatgaact caaagaaatt caaggcatgt ggtttagtag cttggaagaa
     8341 ttactggaaa acaaagagga tattcattgt tatagtgatt gtgattcgat ggaagatgtc
     8401 gcccgttatt acgtggaaga aacaggacag cttggtgaag tcccttccaa tttacaaaat
     8461 tatattgact accaatcttt gggacgtgat atggaaatag agggtaatta cctagttacc
     8521 tctcatgggg tatttgaata ttgtcagtaa gtttaacaat ccgtgttata attaaattaa
     8581 taaatagcac ggagaggtat aaattatggt aaatgtattt gaaaatctta aactattaaa
     8641 actagacatg aaagataaag gctgggtgat agattccttt tactttcgtt acaaacagca
     8701 aaattatatt gtacttgtta agctatttga aaaagaggaa aaagttccag aatatgctct
     8761 gttaaaacta gaatttttaa aggaaaacga tttttcagat atgctagctg tctatgctaa
     8821 ttcggtaaaa ttatatacag acacgaaaac aataagagaa tattttggta tcgaatacag
     8881 tagtaacttg ggagatgttt tatttcaatt tagccaaact cttgctcgat ttattccgac
     8941 agaagttagt gagaagaaaa atgaagacca aaaagaagcc atgtgtttct cattaagtca
     9001 aagtgattca gaagatccca gaaaaaaata ttgtttttca gttagaagaa atcctttaaa
     9061 gggaaatggt gagctgggaa aacgaagtcc ttttaatgac aataaaacga gattgtatag
     9121 acccagctta tatgaacgat tagggtcaga taccaattta agtttcagat attcaatgaa
     9181 tccagatgat gaagaaactg atgaaggtat tattgcaaaa tggacaaaaa ataaaattga
     9241 atagttaaag cattgaaaga gcaatcacct attttgattg ttctttttct atttttacgg
     9301 aaaggacgtg aagacatgaa aaaaataaga agttacacca gtatttggtc agtagaaaaa
     9361 gtgatttatg cgattaatga tttacaactc cctttcccaa ttacctttac gcaaatgaca
     9421 tggtttatcg tttctttgtt tgtcgtcata ctctttggca gtttaccacc actttctctg
     9481 attgacggag cactactcaa atacttaggg attcccattg ttctcactgg gttcatgtgc
     9541 caaaagacct ttgacggaaa gaaaccctat ggatttctaa agtctatcat tacctatgct
     9601 ctacgcccta aagtaaccta cgctggcaaa caaatacgat taaaaaaaga aacaatgaat
     9661 gaatcgatta cgattgtaag gagtgaacaa ctagatggca tatccgatta aatatatgga
     9721 agataatctc gtatttaatc atgatggcga agtattcgct tattatgaat taattcccta
     9781 taattacagt tttttaagtt ccgatgaaaa aattcaagtt catgataatt tctgtcaatt
     9841 gattgcccaa aatcgtgatg gcaagattca tgctctgcaa ctagcaaccg aatcaagtat
     9901 tcgagccaca caggaacaat cgaaaaaaga aatcactgga cgattaaaag agatagctta
     9961 cgaaaaaata gatgaacaga ccgaagcgtt aatttctatg attggtgaca atcaagtaga
    10021 ctatcgcttt tttattggtt ttaaactcta cctcaatgaa caggaagtat ccgtaaaaaa
    10081 tgttgggaaa gaaattcaaa aagcatttgc ggaattcttt catggtgtga atcacaaatt
    10141 aatgggcgat tttgtttcta tgcctatggc ggaaatcgaa cgcttttcta agatggaaca
    10201 gttgttagaa agtaaattat ccagacgctt tcagattcgt aaattggaga aagatgattt
    10261 tggttatatt attgaacatc tttacgggca aacaggtgtc ccttttgatg actacgacta
    10321 ccatttaccg aaacgatacg aagataaaaa agtgttggta aagaaatacg atattctcaa
    10381 acccactcgc tgtttaatcg aagaaaatca acgctattta cggattgaac aggaagatac
    10441 cactacttat gtggcgtatt ttacaattaa tagtattgtt ggtgaattgg atttccctaa
    10501 tagtgaaatt ttttattacc agcaacagca gtttactttc cccgttgata ccagtatgaa
    10561 tgttgaaatt gtcgccaata aaaaagcctt atctaccgtt cgcaataaaa agaaagaact
    10621 gaaagattta gatagccacg catgggaaag cgacaacgaa accagtacta atgtggtgga
    10681 cgctttagaa tctgtcgatg aattagaaag taatttagac caaaccaaag aatctatgta
    10741 caaactaagc tatgtggtac gggtatcagc aaagaatttg gacgagctga aacaacgttg
    10801 tgatgaagta aaagattttt atgatgatct gtctatcaaa ttagtacgac cttttggcga
    10861 tatgctaggc ttgcataatg aatttattcc ctcaagcaag cgatatatca atgactatat
    10921 tcaatatgtc acgtcggatt ttttggctgg tcttggtttc ggtgctacac aaatgattgg
    10981 tgaaaattct ggtatttacg tcggttacaa tctcgataca ggcagaaata tttatctcca
    11041 acctagcctt gcaagtcaag gaattaaagg ctctgtaaca aatgcattag cgtccgcttt
    11101 tattggttca ttaggtggcg gaaaatcatt ctctaacaat atgctggtct attattcagt
    11161 actctttggc ggacaggcaa ccattgttga cccaaaagct gaacgtggtg aatggaaaga
    11221 aaccttacct gatattgccc atgaaatcaa tattgttaat ctcacgagtg agaacgacaa
    11281 taaagggtta cttgaccctt atgtgatttt aaaactcccg aaagattcag aaagccttgc
    11341 cattgatatt ctcacgttct taacaggtat ttccagccga gatagcgaga agtttcctgt
    11401 cctgcgtaaa gctattcgga gtgtaactca aagcaaacaa cgagggttgt tattggtgat
    11461 tgatgaattg cgaaaagaag atacgcccat cagcaatagt attgctgacc atatcgaaag
    11521 ctttgtcgat tatgattttg cccatctgtt attcagtgat ggcagtgtca cccaatccat
    11581 tagcttggaa aaacaactca atattattca agtggcagat ttggtcttac ccgattccaa
    11641 tagctctttt gaagaataca ctaccatgga attattaagt gtggcaatgc tgattgtcat
    11701 tagtaccttt gctttagact ttatccactc tgaccgttcc atttttaaaa ttgttgacct
    11761 tgatgaggct tggagttttt tacaagtggc acaaggaaaa gctctatcta tgcgattggt
    11821 tcgagcagga cgtgccatga atgcaggggt ctatttcgtt actcaaaacg cggacgattt
    11881 actagatgaa aagatgaaga ataatattgg cttgaaattt gcctttcgtt ccactgacct
    11941 agtggaaatc aaaaagacct tagaattttt tggtgtggac aaggaagatg aaaacaatca
    12001 gaaacgctta cgtgaattag aaaatggaca atgtttagtt tcagacatct atggtcgtgt
    12061 aggggttatg cagtttcacc cgatttttga agagttgctt catgcctttg ataccagacc
    12121 gcctgtgcga aatgaggtgg aagaatgaca caaaaacaaa agaaatttct tcactatagt
    12181 tggataacgc tcctttgtgt gggcgttatt ttacttttat taggcacgct gggcaatgca
    12241 gtacaagcga caggcttagt tgatgaaacc attgatacaa gcaatgaata ctccaagtat
    12301 ggtttgaatc aatatcagct tgattattat gtggacaata gctggggctg gcttccatgg
    12361 aattggagtg atggcatagg acaatcggtg atgtatggat tgtatgccat tactaatttt
    12421 atttggacaa tttctcttta tctatcgaac gccacaggtt atttggtgaa agaagcctat
    12481 gccttagatt tcattagtca aacatcccaa gcaattggcg aaaacatgca gacgattgca
    12541 ggcattacac cgaatggctt ttccagttca gggttttatg tcggtttcct ccttcttttt
    12601 attctcattg ttggaatcta tgttgcctat acaggattgg ttaagcgaga aacgacgaaa
    12661 gtgatacgag ctgttttaaa ctttgtcgtg gtgtttattc tatcggcttc ctttattgcg
    12721 tatgcacccg attatattgc gaaaatcaat gatttttcca aagatgtcag taccgccagt
    12781 ctgaatattg ggacaaaaat tgttcttcct aacacggata gtcaagggaa agacagtgtg
    12841 gacatgatac gtgacagctt attctctgtt caagtaaagc aaccatggct attgcttcaa
    12901 tatggctcca ctgatgtaga agcgttaggg gaagaacgtg tggaaagttt attgtctacc
    12961 agtccagata caaacaatgg tgaagataga gaaaatgtag tgattgaaga aattgaagat
    13021 catgacaata cctatttaac cctaccgaag acgatttcca gattaggaac agtcttcttt
    13081 ttgtttgtat tcaatattgg tatctcgatt tttgttttcc ttctaacagg aatcatgatt
    13141 ttttcgcaag tgctatttat tattttcgct atgtttttgc caatcagttt cctattaagt
    13201 atgttgccga gttttgatgg catgggcagg catgccatta ccaagttatt taatgtgatt
    13261 atgacaaggg ctggtattac cttgattatt accgttgcct ttagtatttc cactatgctt
    13321 tattcgctgt cagcaagttc tccctttttc atgattatgt ttttacaaat tgtgaccttt
    13381 gcagggattt attttaaact gggcgattta atgagcctat tcactttaca aagcaacgac
    13441 tctcaaaaca tgggcagaca agtgtttcgc agacctcgtc aaatgatgaa tcgacaaacc
    13501 agaagatttc aacgcagtat gagtaagatt tttcgtggaa aagcagggtc aacgaagcaa
    13561 aagacaatga caacaaagca gaatcaaacc aataaagcta accatactcg tcaaaatgaa
    13621 cggactacac caaagaaaga aacagcactt tccaatgtga aaaagcaaca aggcggtatc
    13681 gggcaacgat taggtgaaaa gacagcccat gtattggaca cgaaaaatcg tctagtcaat
    13741 aaaagaaagc aggcaaaaca acagatacag gcgacaccaa cccatgtcaa atatgccctt
    13801 catcaaggaa aagagaatgt cactcataat gtaacagact ttaaacaatc tatggcagat
    13861 accaaagctc aacgcaaaca acagcgtatg aaccagcagg agcaaaaacg aaagaacatt
    13921 gctcaaaagc gtttggagct ggataaagca aaagaaaaaa gacgaaaaga aaacccatca
    13981 tctcctgttc agaataacca gcagcatgtt cgcaagcgtc agcaaaccgt cacgcctgtt
    14041 ttgtcacaaa acactaaacg tgaaaatcat cagcgaccat tgaaaaaaga gcaaaaacaa
    14101 aagacaccac cgaagcaatt caatcagagg aagctaaata aaagaacgga taagcgagga
    14161 caacaaacat gaagctaaaa ataacagtga tttgtagtgt ggtcttctct ctcttctttt
    14221 ttctcttatt attcctagtc attttctttg cagatgatac ggacagtggc gaaaacaaca
    14281 aggattcttc tattccacaa ggcggtgtga ccgtttcacc agaagtgttg gcacaccgtc
    14341 cgttgattga aaaatatggt aaggaatatg ggattgaaga ctatgtttct tatatcttag
    14401 ccattatgca agtggaatcg ggcggtacag cagaagatgt tatgcaaagt tccgaatctt
    14461 tgggtttacc gcccaacagt ttgagtaccg aagagtccat caaacaagga gtggatatag
    14521 attctgttat ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg
    14581 ggaaaaaata tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa
    14641 aagcagacta tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg
    14701 gaaatcagtt ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat
    14761 ttgatgatga aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct
    14821 atgtctttgg aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg
    14881 tgtatgacaa agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc
    14941 aagaaatctc tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata
    15001 atgcaggcac atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg
    15061 caggcgaccc aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg
    15121 gggctagacg tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag
    15181 cgaaaagaaa agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt
    15241 ccgatgatta aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt
    15301 ggtagtgtcg gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt
    15361 gaaacagaaa tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa
    15421 tcctttgcga aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat
    15481 gaaaaattga cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt
    15541 aaggatattc ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg
    15601 aatgagaaca cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac
    15661 aaggaaacta tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg
    15721 gttattatca aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa
    15781 cagctagaaa gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta
    15841 gaaacgttct tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt
    15901 aatcatgtgt tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc
    15961 tttaccagaa aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa
    16021 acaaaggcaa cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag
    16081 attgtaaaat aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt
    16141 tgataaaaaa ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact
    16201 atttaaaagg agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata
    16261 gcgggaacaa ataattagat gtcctttttt aggagggctt agttttttgt acccagttta
    16321 agaatacctt tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc
    16381 tatggttatg cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct
    16441 tttgggtttt tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca
    16501 tgttgatgc
//
LOCUS       DAZUMV010000050        16470 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000050 DAZUMV010000000
VERSION     DAZUMV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.050"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..549)
                     /locus_tag="ACG8FK_001476"
     CDS             complement(151..549)
                     /locus_tag="ACG8FK_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS5097089.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(546..1319)
                     /locus_tag="ACG8FK_001477"
     CDS             complement(546..1319)
                     /locus_tag="ACG8FK_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS5097090.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1316..1999)
                     /locus_tag="ACG8FK_001478"
     CDS             complement(1316..1999)
                     /locus_tag="ACG8FK_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS5097091.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2166..3053)
                     /locus_tag="ACG8FK_001479"
     CDS             complement(2166..3053)
                     /locus_tag="ACG8FK_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5097092.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3050..3919)
                     /locus_tag="ACG8FK_001480"
     CDS             complement(3050..3919)
                     /locus_tag="ACG8FK_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5097093.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4056..5342)
                     /locus_tag="ACG8FK_001481"
     CDS             complement(4056..5342)
                     /locus_tag="ACG8FK_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5097094.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5615..6193
                     /locus_tag="ACG8FK_001482"
     CDS             5615..6193
                     /locus_tag="ACG8FK_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5097095.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6193..7122
                     /locus_tag="ACG8FK_001483"
     CDS             6193..7122
                     /locus_tag="ACG8FK_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5097096.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7119..8855
                     /locus_tag="ACG8FK_001484"
     CDS             7119..8855
                     /locus_tag="ACG8FK_001484"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5097097.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8918..9754)
                     /gene="pheA"
                     /locus_tag="ACG8FK_001485"
     CDS             complement(8918..9754)
                     /gene="pheA"
                     /locus_tag="ACG8FK_001485"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS5097098.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9751..10260)
                     /locus_tag="ACG8FK_001486"
     CDS             complement(9751..10260)
                     /locus_tag="ACG8FK_001486"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS5097099.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10253..11548)
                     /gene="aroA"
                     /locus_tag="ACG8FK_001487"
     CDS             complement(10253..11548)
                     /gene="aroA"
                     /locus_tag="ACG8FK_001487"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS5097100.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11561..12652)
                     /locus_tag="ACG8FK_001488"
     CDS             complement(11561..12652)
                     /locus_tag="ACG8FK_001488"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS5097101.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12669..13835)
                     /gene="aroC"
                     /locus_tag="ACG8FK_001489"
     CDS             complement(12669..13835)
                     /gene="aroC"
                     /locus_tag="ACG8FK_001489"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS5097102.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13837..14901)
                     /gene="aroB"
                     /locus_tag="ACG8FK_001490"
     CDS             complement(13837..14901)
                     /gene="aroB"
                     /locus_tag="ACG8FK_001490"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS5097103.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14934..15956)
                     /gene="aroF"
                     /locus_tag="ACG8FK_001491"
     CDS             complement(14934..15956)
                     /gene="aroF"
                     /locus_tag="ACG8FK_001491"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS5097104.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4757 a         3632 c         2920 g         5161 t
ORIGIN      
        1 catattttga aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca
       61 taagtattca cgagaaaaat acaaacaaaa aagcggaaat aacgctcatc cgccttcttg
      121 tttgtgttta tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt
      181 ttcatctaaa agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc
      241 aatgactttg tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat
      301 gccttgacca atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac
      361 aatatctttt tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga
      421 acctcttcca tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac
      481 atcttcgccc attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag
      541 atctttcatt tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca
      601 tcttttcgtt tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc
      661 attgcaacca caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt
      721 gccacgctca ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta
      781 tcaaaagcca ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg
      841 tatcctggga tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat
      901 cctactgctg cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc
      961 ccctcgctca attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct
     1021 actaagaacc ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca
     1081 atagcactgc ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa
     1141 aggatcaacc caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg
     1201 cgcatacgaa aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc
     1261 aaaatgactt ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt
     1321 ggaagatcac ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca
     1381 ttccaaaact gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa
     1441 gggccaagat aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa
     1501 tcggcataaa ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag
     1561 ccatcaattg ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata
     1621 ctccagtgcc gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata
     1681 tatcatatac ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc
     1741 cgatagcaaa tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc
     1801 ctactagatc tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc
     1861 cgatcatcat gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg
     1921 caaaaacaaa cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt
     1981 ctttaaaatt ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa
     2041 agagttttcg agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca
     2101 cacgtcccaa aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg
     2161 tttttttatt ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat
     2221 acgatggcag ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt
     2281 gtataggaag cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc
     2341 aaagctacta agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc
     2401 gcggcatagg tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt
     2461 agaccgtcta atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga
     2521 aagaagaaga tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg
     2581 attcccaaaa ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc
     2641 gcaaaaggaa ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt
     2701 tgataaatct caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc
     2761 accaaagcga tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac
     2821 aaattttgaa aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc
     2881 acgggtgtat tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat
     2941 aggatgctta atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct
     3001 tgttttttga cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt
     3061 ctcctcctac tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg
     3121 aaactgctgc cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt
     3181 gagatagggt ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa
     3241 aaagctgcaa tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat
     3301 ttgggatcgt cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt
     3361 cataaatctc cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag
     3421 tccaacgcca agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc
     3481 agccaatcgg tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt
     3541 gagaaaatag acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa
     3601 agatagcggt tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa
     3661 taaaaagtgc tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa
     3721 aaaaagcttt tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa
     3781 atgtcatatt gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa
     3841 taaaaaccat gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga
     3901 ataaatcttt ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa
     3961 gacaagaaag acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga
     4021 tgaaaaagcg aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact
     4081 tgtttttgtg tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga
     4141 gcttcagcta cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt
     4201 tcagatgtcc atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct
     4261 tcttgataag cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc
     4321 gcaagagtgc ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct
     4381 gaatcttctc cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct
     4441 tgcgcttttt gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat
     4501 tggttttctg ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact
     4561 gctcctgatt gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct
     4621 tctttcaaaa gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc
     4681 gtgactgtct ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt
     4741 actcgaccaa ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca
     4801 tctcgcgcta cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa
     4861 cctgcttctg ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga
     4921 acaccgtata ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct
     4981 tcctttacaa tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt
     5041 atcctataat cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa
     5101 gcggtgttca gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt
     5161 tcttcgttct cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc
     5221 cacgcccaga cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct
     5281 gttaagaaac ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc
     5341 atttttatcc gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca
     5401 aaatttgaaa acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa
     5461 aaaaatacaa atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa
     5521 tggtgaggtt tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt
     5581 aaattacaag actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga
     5641 tgatgaacag acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt
     5701 tcaattaata gggacttttt ccagtccgct gaaagcgctt gatttttctt tggtacatgc
     5761 aatcgatatc gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg
     5821 gctattaaaa cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt
     5881 tgatatggtt aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt
     5941 gagtcctgat ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc
     6001 caacaaaagc ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat
     6061 tgctggacaa actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg
     6121 ttatataccg aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc
     6181 acccattcat tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg
     6241 gaagaatccg gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa
     6301 aagtttcgtc aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc
     6361 caagatatca atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat
     6421 cataagggaa agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag
     6481 cagcaagcaa gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa
     6541 gaacttgaga atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa
     6601 catgaggcaa tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg
     6661 aatgaagaac tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat
     6721 ttcgaagatt tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca
     6781 aatgcaaaaa aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat
     6841 tatgagaaag aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat
     6901 ctctcagcca tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc
     6961 cgtatcagta aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt
     7021 gagaaagtcg gctataccga actgggttat ttctccagag tttttaaaga agagacagga
     7081 atgacacctt ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc
     7141 atgaactctt tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg
     7201 tcatttcttt gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat
     7261 cgaatgatgt ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg
     7321 agaatacatt agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc
     7381 cagcccaaca tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc
     7441 tttcaaaaat ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa
     7501 cttttgtcgg aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga
     7561 ttgctcagag agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat
     7621 tgactcttcg cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat
     7681 tgaatcacgt ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt
     7741 ttcaatctgt agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga
     7801 tcatcgcatc aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa
     7861 cagtaggtga aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc
     7921 aggattgtac attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga
     7981 tttttccgat tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta
     8041 tgtatttgga tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag
     8101 aggaagatgg cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag
     8161 ggaatacgat caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag
     8221 acgaaaagaa gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt
     8281 ttatttataa tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag
     8341 cagttcaagg aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag
     8401 aagtgattcc agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta
     8461 tgcgatatgg cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat
     8521 ctatgccgaa tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca
     8581 ataaaacgca aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg
     8641 aagtgattga taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt
     8701 tttcaggaat cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat
     8761 atggcttaac gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc
     8821 ttcttcctaa aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt
     8881 attttaagtt aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg
     8941 tcagcactgg ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga
     9001 gcgcattctc tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt
     9061 ctcccaagct agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc
     9121 caaaagctgc cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga
     9181 cactcatttt tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa
     9241 aacgtgtctg gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat
     9301 gctgtgctgt ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat
     9361 acattgctgc agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt
     9421 ctaaaaactg ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg
     9481 ttgctggatt gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga
     9541 cttctggatg tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct
     9601 ctacaggaac gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga
     9661 tggaagcata cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga
     9721 atgaactttc tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt
     9781 atcgatgatc ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc
     9841 ataccaagaa agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc
     9901 taaaggtcga atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc
     9961 ctctaagtaa agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac
    10021 aatccctcca ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga
    10081 ctccactttt cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg
    10141 gatatgttcc tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa
    10201 ccctttaccg attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat
    10261 gagcttctcc tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg
    10321 aaacagcttc agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca
    10381 gcatcatacc gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg
    10441 gcccatggat gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt
    10501 cttctgccac cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga
    10561 tgatcgtgat tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca
    10621 gacgcggaat gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta
    10681 aatcagccga ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct
    10741 ccaacacatc taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt
    10801 ggctttccgg aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg
    10861 gaacagttat ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt
    10921 ccactgtgat ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt
    10981 ctttttcaac gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt
    11041 tgacttgcgc actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg
    11101 tcacagtgat tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc
    11161 gtaaaggttc catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag
    11221 tagtcgtaaa aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt
    11281 ttcccatgtc aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct
    11341 tgatcacctc ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac
    11401 tcagacaatc ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc
    11461 caaacatgat gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta
    11521 atccatgtat ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat
    11581 aactggtatt agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac
    11641 tgatttgcag tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag
    11701 aaactcccgc ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca
    11761 catcgataaa taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt
    11821 ctcgactttc ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga
    11881 tccattctgc tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct
    11941 gcttattgct caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga
    12001 tatctcgaaa gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt
    12061 ctgcctgatt gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta
    12121 tacggtcatg ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt
    12181 cttgcagttt ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat
    12241 tttcaaatag tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat
    12301 gtccaccgac aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac
    12361 tgctgacatc cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat
    12421 aagctaaggt tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg
    12481 tttcaaaatc acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt
    12541 ttgactgact atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg
    12601 ccatagaagc accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg
    12661 cccccttttt agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca
    12721 acaagcgaat cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca
    12781 ttttccgcaa cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct
    12841 ttgtaaggtt cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg
    12901 ggtttcatga ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg
    12961 cctagattat tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc
    13021 acttctgagc ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag
    13081 gcattgatac taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg
    13141 tagcttccta atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta
    13201 tctccatttt tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca
    13261 acaactcgta cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca
    13321 ggtatttctg cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt
    13381 tcttccagaa ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca
    13441 gaggaccgtt caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa
    13501 tccgcgtgtc ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact
    13561 tctgctacag acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg
    13621 acaggtgaac caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt
    13681 tcgatcttca tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg
    13741 atttcttctt ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct
    13801 ggtccatggg attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt
    13861 gaatgtctct gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat
    13921 attcaattgg cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg
    13981 atgacttaga tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc
    14041 tgtcgatcca gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc
    14101 aatagctact ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg
    14161 gccaattgta tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc
    14221 cactactttt cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag
    14281 atctttttcg tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc
    14341 tgatttgatg atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac
    14401 ctcaggatca atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt
    14461 gtttgtattg actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt
    14521 cggaatttga acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc
    14581 acccaccatt cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc
    14641 taaaaaatcg tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac
    14701 gattgaaagg actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata
    14761 tagtggagca acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg
    14821 aagccatgat ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt
    14881 atcaacatgt acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg
    14941 agtgtctgaa tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag
    15001 taagtctttt cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg
    15061 atctctacga tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca
    15121 agttcccaaa ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt
    15181 ttcatcaatg ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga
    15241 atcccacgtt cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca
    15301 ttaagccatt cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt
    15361 ttaccgacag cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata
    15421 tcgctgtact cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc
    15481 attccaaatt catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg
    15541 aaagcatatg gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg
    15601 gctttggcca ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca
    15661 gccatcgtca cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg
    15721 ccaggatgga attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct
    15781 actccttcat agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca
    15841 atcactactc gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga
    15901 tcgacgactt tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt
    15961 tttcccgctt tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt
    16021 ggttttacat taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct
    16081 ttcctactta tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta
    16141 cagtgaacat cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag
    16201 atgaatttct ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa
    16261 acctggtttg tttgtaccca cgaagtcatg aatacaaacc tatctaaaat agctaaagta
    16321 atacgtattc agttttgtaa gttttctaga tttcataaaa aatcgctcca aactgatgta
    16381 agaattgtac ctagtataga cataaaaaaa tcgactgtca atcattttta tgaaactttt
    16441 tttaattttc tgatagttaa ctttgttttt
//
LOCUS       DAZUMV010000051        16045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000051 DAZUMV010000000
VERSION     DAZUMV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.051"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8FK_001492"
     CDS             226..894
                     /locus_tag="ACG8FK_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS5097105.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="ACG8FK_001493"
     CDS             complement(1094..1480)
                     /locus_tag="ACG8FK_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097106.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="ACG8FK_001494"
     CDS             complement(1452..2834)
                     /locus_tag="ACG8FK_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5097107.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="ACG8FK_001495"
     CDS             complement(2990..4177)
                     /locus_tag="ACG8FK_001495"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS5097108.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="ACG8FK_001496"
     CDS             complement(4184..5215)
                     /locus_tag="ACG8FK_001496"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5097109.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="ACG8FK_001497"
     CDS             complement(5231..6685)
                     /locus_tag="ACG8FK_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS5097110.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPTLSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVASASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="ACG8FK_001498"
     CDS             6860..7765
                     /locus_tag="ACG8FK_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS5097111.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENDLQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTGESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="ACG8FK_001499"
     CDS             7856..8533
                     /locus_tag="ACG8FK_001499"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5097112.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="ACG8FK_001500"
     CDS             8530..8700
                     /locus_tag="ACG8FK_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097113.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="ACG8FK_001501"
     CDS             8814..9710
                     /locus_tag="ACG8FK_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5097114.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="ACG8FK_001502"
     CDS             complement(9765..11135)
                     /locus_tag="ACG8FK_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5097115.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="ACG8FK_001503"
     CDS             11373..12119
                     /locus_tag="ACG8FK_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5097116.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="ACG8FK_001504"
     CDS             12116..12955
                     /locus_tag="ACG8FK_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5097117.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="ACG8FK_001505"
     CDS             12956..13906
                     /locus_tag="ACG8FK_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5097118.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="ACG8FK_001506"
     CDS             14047..14799
                     /locus_tag="ACG8FK_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097119.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="ACG8FK_001507"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="ACG8FK_001507"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS5097120.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5200 a         3002 c         3229 g         4614 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagtg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgctagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagt tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg atttacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacgggt gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttaca
//
LOCUS       DAZUMV010000052        15900 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000052 DAZUMV010000000
VERSION     DAZUMV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.052"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACG8FK_001508"
     CDS             complement(52..522)
                     /locus_tag="ACG8FK_001508"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS5097121.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACG8FK_001509"
     CDS             complement(681..1139)
                     /locus_tag="ACG8FK_001509"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS5097122.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHENYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACG8FK_001510"
     CDS             complement(1310..2455)
                     /locus_tag="ACG8FK_001510"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS5097123.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKRDEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACG8FK_001511"
     CDS             complement(2468..3385)
                     /locus_tag="ACG8FK_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS5097124.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACG8FK_001512"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACG8FK_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS5097125.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="ACG8FK_001513"
     CDS             4477..4647
                     /locus_tag="ACG8FK_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097126.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="ACG8FK_001514"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="ACG8FK_001514"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS5097127.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="ACG8FK_001515"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="ACG8FK_001515"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS5097128.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /gene="dapA"
                     /locus_tag="ACG8FK_001516"
     CDS             6911..7792
                     /gene="dapA"
                     /locus_tag="ACG8FK_001516"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS5097129.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /gene="dapB"
                     /locus_tag="ACG8FK_001517"
     CDS             7789..8505
                     /gene="dapB"
                     /locus_tag="ACG8FK_001517"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS5097130.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="ACG8FK_001518"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="ACG8FK_001518"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS5097131.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="ACG8FK_001519"
     CDS             9222..10223
                     /locus_tag="ACG8FK_001519"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS5097132.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="ACG8FK_001520"
     CDS             10220..11581
                     /locus_tag="ACG8FK_001520"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS5097133.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="ACG8FK_001521"
     CDS             11541..12692
                     /locus_tag="ACG8FK_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5097134.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="ACG8FK_001522"
     CDS             12667..13836
                     /locus_tag="ACG8FK_001522"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5097135.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="ACG8FK_001523"
     CDS             14011..15390
                     /locus_tag="ACG8FK_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5097136.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="ACG8FK_001524"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="ACG8FK_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5203 a         2802 c         3384 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gttttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcatctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 cgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagatt atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DAZUMV010000053        15821 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000053 DAZUMV010000000
VERSION     DAZUMV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.053"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..802
                     /locus_tag="ACG8FK_001525"
     CDS             50..802
                     /locus_tag="ACG8FK_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5097137.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            799..1713
                     /gene="pfkB"
                     /locus_tag="ACG8FK_001526"
     CDS             799..1713
                     /gene="pfkB"
                     /locus_tag="ACG8FK_001526"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5097138.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1731..3662
                     /locus_tag="ACG8FK_001527"
     CDS             1731..3662
                     /locus_tag="ACG8FK_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS5097139.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3773..5332)
                     /locus_tag="ACG8FK_001528"
     CDS             complement(3773..5332)
                     /locus_tag="ACG8FK_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS5097140.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5487..7721
                     /gene="pcrA"
                     /locus_tag="ACG8FK_001529"
     CDS             5487..7721
                     /gene="pcrA"
                     /locus_tag="ACG8FK_001529"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS5097141.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7738..9774
                     /gene="ligA"
                     /locus_tag="ACG8FK_001530"
     CDS             7738..9774
                     /gene="ligA"
                     /locus_tag="ACG8FK_001530"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS5097142.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9880..10185
                     /gene="gatC"
                     /locus_tag="ACG8FK_001531"
     CDS             9880..10185
                     /gene="gatC"
                     /locus_tag="ACG8FK_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS5097143.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10185..11657
                     /gene="gatA"
                     /locus_tag="ACG8FK_001532"
     CDS             10185..11657
                     /gene="gatA"
                     /locus_tag="ACG8FK_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS5097144.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11654..13084
                     /gene="gatB"
                     /locus_tag="ACG8FK_001533"
     CDS             11654..13084
                     /gene="gatB"
                     /locus_tag="ACG8FK_001533"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS5097145.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13094..14149
                     /locus_tag="ACG8FK_001534"
     CDS             13094..14149
                     /locus_tag="ACG8FK_001534"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5097146.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14260..15633
                     /gene="rlmD"
                     /locus_tag="ACG8FK_001535"
     CDS             14260..15633
                     /gene="rlmD"
                     /locus_tag="ACG8FK_001535"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5097147.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15636..15764
                     /locus_tag="ACG8FK_001536"
     CDS             15636..15764
                     /locus_tag="ACG8FK_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5097148.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5383 a         2899 c         3474 g         4065 t
ORIGIN      
        1 gcgagtagaa aaaaaatatg ctattatttg tattgaggtg atcgtgaaaa tgcttacaga
       61 ggaacgtaga caaaaaatcc tagacttgct tgaacagcag aaaatcgtga aatcacaaga
      121 tcttgtaaac ctttttaatg cttctgaatc aacgataaga cgtgatttac aagagctgga
      181 gaatagtggg tttctagaaa gagttcatgg cggagctaag aaagagcaat tattgaattt
      241 tgagcagaat atgagtgaaa aatcactcaa aaacgttcac gaaaaacagc aaatcgccaa
      301 atcggcagct gaaaccatta atgacgaaga tgtcatttat ttagacgctg gctctactac
      361 gttagaaatg attccttttc tgcaaggaaa acagatcaca gtagtgacaa attccgttca
      421 acatgcggca aatttagttg atttggagct tgctaccatt attttaggtg gaacgataaa
      481 gctggcaact aatgctgttt taggatctaa tgcgatagaa caactaaaaa attttcattt
      541 taataaagca tttatgggaa tgaacggtgc ccatcttgaa caaggattca cgactccgga
      601 cccagaagag gcagctgtta aacgtttagc catgaaaaat tccgaagaga catttgtatt
      661 gattgatcat accaaattca accagttgtc cttcacctct gttgcacctt tggaagcagc
      721 cacgatcata accgagcgtt gtccattaga attcgttaat gaattccgtg aaaagacaac
      781 aattaaggag gcaatacaat gatttacaca gtaacactta atccatcaat tgattatatc
      841 gtacgcgtag atgggttaaa actaggcgat ttgaaccgaa tgaaggaaga cttcaagtta
      901 cctggaggaa aagggatcaa tgtttcccgt attttgaaac ggatcgattc tcaatctaca
      961 gcactagggt ttttaggagg gttcactggc gaatttattt cgaagtggct tgaaaaagaa
     1021 caaatccagt ctttgttcac aaccgtaaaa gaagatacaa gaatcaatat caaactaaaa
     1081 tcagatactg aaactgaaat taacggtttg ggcccatcta tttcagatga agaaatcgat
     1141 gagttgaagc aagcattagg aacagtagga caaggagata tcgtcgtatt gtcaggaagc
     1201 gcgccggcta gcctgcgaaa aggattttat caagaactga ttgatatcat tcgagaaaaa
     1261 ggtgcagatt tcgtgattga tacaacaggt gatgacctaa aaaatgcact tgttaagaaa
     1321 ccattgttga tcaaaccaaa caatcacgaa ttagctgagc tttacaatac aaccttccaa
     1381 tctgtagaag aaatcattcc gtatggtaaa aaattgctag ctgaaggggc acagaatgtg
     1441 ctgatctcaa tggctggaga cggtgctttg ctcttcacgg aacaaggaac ttataaaacg
     1501 aatgtattag ttcgcccatt gaaaaattca gtaggtgcag gggattcaat gattgccggc
     1561 tttattgggg cttacagccg tacgaaagat ccaatcgaag cctttaaatg gggagttgct
     1621 tgcggaagcg caacagcttt ttctgatgac ttggcagctc gcgaattgat tgatgagttg
     1681 ctgccagaag ttgaaatcac aaaaattgat tagtttcaag gaggataatc atgaatatca
     1741 aggatttaat ggtcaaagaa gcgatgatca tggatttgca agcgactgat aaaaaaggcg
     1801 caatcgatga aatggtccaa aaaatgtttg atgccggccg gatttccgat atcgaaacat
     1861 ataaagaagg aattctagcc cgcgaagcac aaacttctac aggtcttggt gatggaatcg
     1921 ccatgcctca cgcgaaaaac agtgcagtta aagaagcaac agttttattc gcaaaaagcc
     1981 aaaaaggagt agattatgaa gccttagacg gacagccaac ttatctgttt ttcatgatcg
     2041 cagctccaga aggcgcaaac gacacacatt tgcaagcatt agcagcacta tctcgattgc
     2101 tgattgatgc agatttcgta ggaaaattaa aagaagcaaa tacaccagaa gaagtccaag
     2161 acttgttcca aacagctgaa gagcaaaaag aagcagaaga aaaagcagaa caagaaacac
     2221 aagctgcaga agcttctgct catccagaaa gaaaattcgt tgttgccgtt acagcttgtc
     2281 caacagggat cgctcacact tacatggcag aagatgctct gaagaaaaaa gcgaaagaaa
     2341 tgggtgtgga gatcaaagtc gaaacgaacg gttcagaagg aatcaaaaac cgattgactg
     2401 ctgaagacat cgcccgtgca gaaggtgtaa tcgttgcagc agacaaaaaa gtagagatga
     2461 atcgctttga cggaaaagaa ctgatcaatc gtcctgtcag tgatggtatc cgtaaaccag
     2521 aagagctgat caatctagcc cttagtggta ctgcaccagt attccatggt gatggaaaag
     2581 aaagcagttc agaagaagga aatgcggatg gtactatcgg acaaagaatt tacaaagatt
     2641 tgatgaacgg cgtatcacac atgttacctt tcgttattgg tggaggaatc gctattgcgc
     2701 tttctttcat gatcgaccaa tttattggcg tgccacatga tcaattatca aatctaggga
     2761 attacaatca agctgctagc tggttcaacc aaatcggaca agctgcattt ggtttcatgc
     2821 tgccggtatt agcaggattt attgcttcaa gtatcggtga ccgccctggt ttgatcgttg
     2881 gatttgctgc tggtgcatta gctaatacag gcggtgcagg attcttaggt gcactgattg
     2941 gtggattctt agctggttat gtcatcgtat ttttacgtaa attattcaag aacttaccaa
     3001 aatcattgga tggaattaaa acgatcctct tttatccagt gtttggctta ctgattactg
     3061 gtttcttgat gttgctggtc aatgtcccta tgaaagcaat caatgatgca ttgaacggtt
     3121 tcttgacaag tatgagtggt tcgaatgctg ctttacttgg tgcattatta gctggaatga
     3181 tggcggcaga tttaggcggt ccaatcaaca aagccgctta tgtgtttggt acagcaactt
     3241 tagctacgac tgttgcaact ggcggaagtg tcgtgatggc ttctgttatg gctggaggaa
     3301 tggtacctcc attggcaatt ttcgttgcta cacgtttgtt taagaataaa ttcagcaaaa
     3361 cagatcaaga tgctggtttg acgaacatcg tgatgggact atcgtttgtc acggaaggat
     3421 cgattccatt tgcagcagct gacccgattc gtgccattcc aagttttatt attggttctg
     3481 ctttaactgg cggattagtc ggagctttcg ggatcaaatt actggctcct catggcggga
     3541 ttttcgttgt attcttactg agtcacccgg tcatgtattt ggtgtttatt gctatcggag
     3601 cagtcgtatc tggtgtgatt tatggtttat tgagaaaagc tcctgcagca gcagaagcat
     3661 agaaataaaa aagaagaggc tggtttttcc cagtctcttc tttttttata gaatcatata
     3721 gaatcaagta gtgtagatga atagttgttg taaaatagtt tttccatgtt cgttaaaatg
     3781 attgcttcaa atttgtgata gaagatttag cctcgatttc ctgcttgatt cttggcaagt
     3841 ttgcttcatc agtcaagata accaaaatat ctcctacatt catgatcgta tcaccgtgag
     3901 tcaagatttc ctgatttcct ctgcgtatag agattagcag catattttta ggccaattga
     3961 agtctcgtac cattaatccg tctaaagaac tctctgccgt tactggaaaa tcaaaagttg
     4021 tccttgcacc ttttagtgag gcggtctgag tatttccaag tagacgttcc aataatgatt
     4081 cgtaaattgg attaccgccg agaaggtcat tgacgatata agcaacaaga gcagccaatc
     4141 ctaaaggcat cagatgatta agagagccaa ccatttctgt cactaaaata attgctgtca
     4201 gcggtgcttt tccaatcgct gtgaaataac cagccatggc aataacgaca aaatccttga
     4261 tgtagattgg atccatcccg aaatcaacca taatgtttcc ataaaagcta ccgattaaag
     4321 cccccaatgt caatatcgga aggaaaatcc cccctggaag accaccaccg taagaaatca
     4381 tggaaaaaac gaatcgaaga acaaacaaac cgataatcat aatgattggc aaggaagcat
     4441 gtccaaaagc taaaacgatt tgattgcctc cgcctagagt actaggccat aaataaccaa
     4501 ttggcaaaat cagcaagaaa ggaacgaacc cgtgaaaata agctggtaaa ggcaatcggt
     4561 tgtaaaattt tggcaaagcc agcaagacct tctgataaat aaaacctaag atgcctagaa
     4621 gtactcctaa gatcactaag gaaccataat atttcaacgg caaagaagga aggtcaccta
     4681 gatataaaac aggtctcaag ccgaaaaagt aaagagagac gaagttagca gtgattgctg
     4741 aagcaaatga tgtcagccaa acaagtggag aaaaggagtg atggatttct tctaaaacaa
     4801 agagtaatcc tgcgatagga gcattgaatg ctgctgcaag tccgctactt gcaccgctag
     4861 aaattaaaat cttttcttct gaagatggac tccgaaacaa ttgtccagct ccttgtccaa
     4921 tggatgcgcc aagctgtata gaaggtcctt ctctacctag aaaaaggccc gcaccaactg
     4981 aaagtacacc gccaatgaac tttttccata aaacagagaa ccagttcatt gataattccc
     5041 cgcgaagctg tccctctact tgtggaatcc cgcttccctt gatatccggt tcatttttga
     5101 ccagataacc cacgaaaaat gctgcaagga tgcagcaaat agctaggcca attaagtaaa
     5161 tcggctgttc ccgactcatt tcatagatac ttcgaactaa atcgcttagc ttttcgatag
     5221 ataatcgaaa caggctaacg acgattcctg tcagcagtcc aacaaatatt ccttttagaa
     5281 taaaatatat ctgagtctta tctaatttct tgatttttcc ttttttttcc attcaaacgc
     5341 ctcgctttct tttaatttag cgcgaaatca acgaattgac aaggaaaaat ccataaaaag
     5401 aaacaaatga ttttgaacta gctagtttaa tgaaagccaa gaaaatgcta taattaacaa
     5461 gattgataaa agaaaggatg ttgttaatgt catccaaagc tgaattatta aacgggatga
     5521 acccccgtca gaaagaagct gttcttcaca ctgacggacc attactgcta atggcaggtg
     5581 caggaagtgg aaagacgaga gtgctgacac accgcattgc ctatttgatt gaggaaaaag
     5641 aagtcaatcc ttggaacatc ctagcgatta cttttaccaa taaggctgca aaagaaatga
     5701 aagaacgtgt caatgcgatt ctcgcatctg gaggcgaaga tgtctgggta tcgacttttc
     5761 actcgatgtg tgtgcgtatt ttaagaagag atgttgattt tatcgggtat aaccggaact
     5821 ttacaatcat cgattcttct gaacagctga ccttgatgaa acggatacta aaagaactga
     5881 acattgatcc gaaaaaatat gatcctcgaa gtatattagg aacaatctct caagcaaaaa
     5941 acagcctgca gacccctcag gattttgcaa aaatgcaagg cagttattac gaagagattg
     6001 cggcaaaatg ttatgctgct taccaaaaag agcttcaata taatcaatgt atggactttg
     6061 acgacctgat catgaatacg attcgattat ttgaagaaca tcctgattca ctcacatatt
     6121 atcaaaacaa attccattat attcatgtag atgagtacca agatacaaat cacgcacagt
     6181 atacgctagt caatttattg gcaggtagat ttaggaacct gtgcgtcgtg ggagatgcag
     6241 atcaaagtat ttacggctgg cgcggtgcag atatgcagaa catcttggat tttgaaaaag
     6301 attatccaga tgctgcagta attttgctag aacaaaacta tcgctccacg aaaaacatcc
     6361 ttagcgcagc aaaccaagtc attgaaaaca atagcaatcg aaaaccaaaa aacttgtgga
     6421 cagaaaacaa agaaggaaac aagatcactt actaccgtgc agataatgaa cgagacgaaa
     6481 cccgttttat cgtagatcgg atgcaagaag agattcgaag caatcatcgg aattacggcg
     6541 actttgccat cttgtaccgg acgaacgcac aatctcgtgt aatggaagaa actctgttga
     6601 aagcaaatat tccttataaa atggttggag ggcacaagtt ctacgatcgt aaagaaatca
     6661 aagatatttt agcgtatttg aatgtgctgg caaatcctca agattcaatc agttttgagc
     6721 gtatcgttaa ttcgcccaaa cgaggcatcg gaccaggatc tatcgaaaaa ctacgcagtt
     6781 ttgcttctct tcatgaatgg ccactgcttg aagcagcgca aaatgtggat ctggcaaata
     6841 tcggtggaaa agcaggacag caattaggtg catttggaga gatgatccaa gaagtcactc
     6901 agatgatccc atacttgaca gtgacagaat tgacgaaaga ggttttggat cgtagcggat
     6961 atctagaaga cttaaaaatc caaaatacat tagaagcaca agcacgcatc gaaaacttgg
     7021 aggaattttt aacggtcaca caagaattcg acaaacaatt cgaacagcaa aatgaagaag
     7081 acgctgatgc accagaagaa aaactaacgg tcttcttaaa tgatctggca cttgtttccg
     7141 atattgacaa tttagaagaa gatgcttccc aagtgacatt gatgactttg catgcagcca
     7201 aaggattaga attcccagtt gtattcctga ttggtctaga agagggggtt tttcctttat
     7261 ctcgagcatt gatggaagaa agcgaactgg aagaagaacg ccgtctcgca tatgtgggga
     7321 tcacgcgagc agaagaagcg ttgtatttaa caaatgcttt ctccagaaca ttatatggac
     7381 ggacgcaata taaccgacct tctcgttttg tagaagaaat cgatcaggag ttactggaaa
     7441 tcgaaggcat gcgtcctacg cctaaaaaaa cacctgtatt cgctaaaaaa acagcatata
     7501 gctataaaca gccagagaca gcggttgttc cctctaaaag tgctactgga ggggaaaata
     7561 acagctggaa acctggagat aaagtcaaac acaaaaaatg gggtctagga acagtcgtcc
     7621 gagtaagcgg gacgtcgaag gatctagaac tagatgtggc tttcccaagt caaggagtaa
     7681 aacgtctttt ggctgccttt gcgccaattg aaaaagctta gtagtggagg gtaaatgatg
     7741 gaacatgaaa tgacgattga cgaagctgct aaacgagcag aagaacttcg tacaagactg
     7801 aaccaatggt ctcgcgaata ctacgtagaa gataaaccaa cagtggaaga ctatgtttat
     7861 gataaagaat acgcagaatt agtagcaatt gaggaacaat atcctgattt gatcacgtct
     7921 gattctccca ctcaacgagt aggcggaaaa gtattagaag gttttgaaaa agtcactcat
     7981 gatattccgc tttacagttt gaacgacgtt ttcagcaaag aagagctgat agcttttgat
     8041 caacgtgtac aaaaagcagt gggacgtgta gttgactatt gctgtgaact aaaaatagat
     8101 ggactttccg tttctttgcg ttatgaagat ggaaatttcg tgagaggtgc gactcgcggg
     8161 gatggaactg taggagaaaa catcactgaa aacctaaaaa ctgtccggtc ggtcccaatc
     8221 aaattaaaag aaccaatgaa catcgaagta agaggcgaat gtttcatgcc aaaacggtca
     8281 tttgtccaat tgaaccaaga cagagaagca gagggaaaag atatctttgc gaatcctcgg
     8341 aacgccgcag ctggttcttt gcgtcagtta gattctaaaa tcacagcgaa aagaaatctg
     8401 gacacttttt tatacacagt agctgatttc ggtcctatgc aagcaaaaac ccagtatgat
     8461 gcgctagaag agctagaaaa aattggattc cacacgaatc gggaaaaacg tctatgtcat
     8521 tcgattgatg aagtgtggtc atacattgaa gaatatcatg acaaacgtgt ggatttgccc
     8581 tatgaaatcg atggaatcgt catcaaagtg aatgaattct cgctacaaga tcaacttggt
     8641 tttactgtca aagcaccgag atgggcagca gcttacaagt ttccgccaga agaagttgaa
     8701 acgctaatcg aaaacatcga atggacagtc ggtcgaacag gggtagtaac gccaacagct
     8761 atcatgacac cagtccgagt agcaggaacg acagtaagcc gtgccagcct gcataacggt
     8821 gattatatta agttgaagga tatccgcttg aaagatacag ttctgattta taaagcggga
     8881 gacatcatcc cagaagtttc gcaagtcgtg ttagataaac gacctaaaga cagcgaagaa
     8941 taccaattgc ctacacattg tccagtttgt ggaagtgaac ttgtccattt ggatgaagaa
     9001 gtagccttac ggtgcatcaa tcctaaatgc cctgctcaaa tgaaagaagg tttgaaccac
     9061 tttgtttcaa gaaatgcgat gaatatcgat gggttggggc cgcgcgtact agaacagatg
     9121 tatgataaaa aactagtagc agatgttgct gatctttaca aattgacaga agaagagtta
     9181 ttgacgttgg acaaaataaa agaaaaatca gcaaataata ttttgacagc aatcgacaac
     9241 agcaaagata attcggtcga gcggttgatt ttcgggttag gaatccgaca tgtcggagcg
     9301 aaagcagcaa aaattttagc agaacatttc ggtgatctag aaacattgag taaaagtgac
     9361 tatgagtcga ttattgcgct tgatacgatc ggtgatatca ttgccgacag tgtagtcact
     9421 tatttcagta acgaagaagt ccatgaattg atgaatgaat taaaacaagc aggcgtcaat
     9481 ttcgaataca aagggcttcg aaatgcccaa ttgcaagaag tcgagtctcc ttttaaagaa
     9541 aaaacagtag tccttactgg taaactaaca cgatttactc gtgaagaagc aaaagagacg
     9601 atcgaaaatc ttggcgggaa agtgactggt agcgtatcga aaaaaacgga tatcgtggta
     9661 gcaggagaag atgctggcag caagctgacc aaagcacaag aattaggaat cgaagtttgg
     9721 acagaagatc aaatggcaga tgcattggct aaaagtcgct cagtagaaga atgaaaaatg
     9781 agttttttca gacaaatttc aataaacaat ttcagacacg gcactcttgt gttaaaatag
     9841 agagcaaagt ttttaactcg tttagaaaga aggggtagta tggcgattag tgaagaacaa
     9901 gtgaaacacg ttgccaaatt agcaaaactt gcgttcgcag atgaagaact tgcaagtttt
     9961 accgaccaat taggtaaaat catcgatatg gttgaaatgc tggaagaagt agatacagaa
    10021 ggtgttccat ttacgtcgaa tgtggttgaa acagtaaacg tgatgcgaca agatcgggcg
    10081 caagctggat ggaaccgcga tgaattattg aaaaatgtgc cggaaacaga agacggcttt
    10141 atcaaagtac ccgcaatcat cgataatggg gaggctggcg cataatgaca gaattacaca
    10201 atcgttctct agaagaactg catcatatgc ttgtatcgaa agaaatcact gtacaagatt
    10261 tgacaaaagc aacatttgaa cgaatcaaag aaacagagcc tgaggtcgaa gcattcatta
    10321 cattgaacga agaaaaagca ttggaacagg caaaagcttt agatgaaaaa gggattgatg
    10381 aaagcaacgt ccttgctggt attccaattg gtatcaagga taatatcgta acaaaagata
    10441 tcttgacaac cgctgcatct aagatccttt acaattttga accaatttat gatgcaacgg
    10501 tcatggataa aatctatcaa gcagatatga tccctgttgg taagctaaac atggatgaat
    10561 ttgctatggg tggaagtaca gaaacttctt atttcaagaa aacaaaaaat gcttgggacc
    10621 caacaaaagt acctggtggc tcttctggtg gttctgcagc agcagttgca gctggacaaa
    10681 ttccagtttc tttaggtagt gatactgggg gaagtatccg ccaaccagca gcttttaacg
    10741 gcatcgtagg gatgaaacct acttatggcc gtgtctcacg ttttggtttg attgcctttg
    10801 cttcatctct tgaccaaatc ggaccaatga cccgtacggt gaaagataat gcattagcat
    10861 tgacagctat cagcggttat gacgaaaaag acggtacttc ttcaggcgtt tcagttcctg
    10921 attttgcaga gggactgaca ggcgacatca aaggaatgaa gattgcttta cctaaagaat
    10981 acttaggcga aggtgtggcc cctggcgtaa aagaagctgt attgaaagca gcagaaacat
    11041 tccgttcatt aggtgctgtt gtggaagaag tcagcttgcc tcattcaaaa tacggtgtgg
    11101 ctgtttatta tatcattgcc tcatcagaag caagttccaa cttgcaacga ttcgatggta
    11161 tccgctacgg ttatcgctca gaaaatgtga aatcattaga tgatgtatat gtcaactcac
    11221 gttcagaagg attcggtgaa gaagtaaaac gccgtatcat gttaggtaca ttctccttaa
    11281 gtgctggtta ttatgacgct tacttcaaaa aagcgggaca agtccgtaca ttgatcaaac
    11341 gagattttga aaaagtcttt gcagattatg acttgatcat cgggccatct tcaccaacag
    11401 ttgcattcgg aatcggtgag aacattaacg atccaatcac aatgtacatg agtgacatct
    11461 taacgatccc tgtcaacttg gctggacttc caggtatgtc tgtaccggca ggtttatcag
    11521 aaggattacc agttggattg cagatcatcg gtaattattt tgatgaaaaa acgatgtatc
    11581 aagcggcata tgcgtttgaa caagcaacag aattccataa acaacaacca gctattttag
    11641 gagggaaaga cgaatgaatt ttgaaacagt cattggacta gaagtccacg tcgagttaaa
    11701 aaccaactca aaaattttct ctccagcgcc agctcatttt ggtgcagaac caaacagcaa
    11761 tacaaacgtg atcgactggg gttatccagg cgtgctgcca gtaatgaata aagctgcgct
    11821 ggaattcggg atgaaagcag ctttggcatt aaactgtgaa atctctaaag atactcattt
    11881 tgaccggaaa aattactttt acccagataa tccaaaagca tatcaaattt cccaatttga
    11941 tcaaccaatc ggtcatgacg gatggatcga aatcgaagta gaaggtaaaa agaaaaaaat
    12001 ccggatcgaa cgtgtccatt tagaagaaga tgctggaaaa aacatgcatg gtattggcgg
    12061 ttattcttac gttgatttga accgacaagg cacgcctttg atcgaaatcg tttctgaagc
    12121 tgatatgcgt tcaccagaag aagcatacgc ttatctagaa gcattgcgat caattatcca
    12181 atttacagaa gtcagcgatg tgaaaatgga agaaggctct atgcgctgtg atgccaatat
    12241 ttctcttcgc ccttatggac aagaagagtt cggtacgaaa gcagaattga aaaacttgaa
    12301 ctctttatct ttcgtgaaaa aaggtttagc ttttgaagaa aaacgtcaag caaaagttct
    12361 tctttcagga ggagaaatcc aacaagaaac acgccgtttt gacgaaacaa cgaataaaac
    12421 agttttgatg cgtgtcaaag aagggtctag tgattatcgt tacttcccag agcctgatat
    12481 cccgaaattc gtgatcgatg acgaatggat tcaaaaagta aaagctagtt tgcctgagat
    12541 gccagcttct cgtcgcgaac gctatatccg tgagtttgga cttccagagt atgacgcaat
    12601 ggtattgaca cttacaaaag aaatgtctga tttttttgaa gctgttttgg aaaatggtgc
    12661 agatgccaag caagcatcta actggttgat gggtgaagtt tctgcttact tgaacagtga
    12721 gaaactggaa ttagctgaga caaaattgac acctgagaac ttagcaggaa tgattcgttt
    12781 gatcgccgat ggaacgatca gctcaaaaat cgccaaaaaa gtcttccgtg aattgatcac
    12841 aaatggcgga gaagcccaag aagtagttga aaaaaatggt tgggtgcaat tatctgatcc
    12901 gaacaaacta ctgccaatca ttaatgaaat tttggataac aaccaacaat ctgtcgatga
    12961 tttcaaaaat ggaaaagatc gtgcggttgg cttcctagtc ggtcaaatca tgaaagcaac
    13021 taaaggacaa gcaaatcctg gtgtcgtgaa taagctactt aaagatgaat tagcgaaacg
    13081 atagaggctg aaaatgaaaa aagcacgcgt aatttataac cccacctcgg gaaaagaatt
    13141 attgaaaaaa aatttggcgg atatcctaca ggcattagaa gaagcaggat ttgaagccag
    13201 cgcctatgca actacaccag aaccagattc agccaagaat gaagcatgta gagtcgcaga
    13261 actaggtttt gatctagtag tggcagctgg tggcgatggc acaatcaacg aagtggttaa
    13321 tggaatcgct ccgcttgaac accgtcctaa aatggccatc atcccagctg gtacaacgaa
    13381 cgactatgct cgagcattaa aaattcctag agataatatt aaagcagcag cagaagtgat
    13441 aaaaaaacac caaaccgtaa aaatggacat cggcctttct gataacagtt acttcatcaa
    13501 tatcgctgca ggtggctatt tgacagaatt gacttatgaa gttccatcag aactaaagag
    13561 tatttttggt tatcttgctt atcttgcgaa aggagcagag atgctccctc gagtaaagcc
    13621 aatcaaaatg cgcttgaagt atgatgaagg ggaatatatc ggaaatgctt cgatgttttt
    13681 cctagggctg acgaactctg ttggaggatt cgaacagatc gcgccagatg ccaaattgga
    13741 tgatgggaag ttttctttaa tcattgtgaa gacagcaaat atctttgaga tcttgcatat
    13801 tgcagcactc atgctgaatg gcggtcgaca tgtcgatgat ccaagaataa tctataccaa
    13861 aacaagcagt ttgttcgttg aaacagaaga tgatatcaat cgaccagtaa tgatcaactt
    13921 agatggtgaa tacggcgggg aagcaccgat gcatttccaa aatcttcatc aacacattga
    13981 gttctttgcc aataccgatc agattcctga tgaagcaatc acaggtacag atgaagaaga
    14041 attagaggtt gtcagcaaag aattcgtaaa agaagtcgaa cgtttaacgg atgaagatat
    14101 cgacggtgac ggaaaaatag cagacgaaac acctaatcaa aagaattaat caaaagccgg
    14161 tgctgaacgt agtaaggtca gtgccggttt tagttgtgta attagctgat ttatagtaga
    14221 gttcagtagg attgaaaaat aggcttaaag gagcaaaata tgacagaaca gcaaatagta
    14281 aagaaaaacg aacgatatac agtcgaaatc gtcgacttga gttatgaagg aatgggggta
    14341 gcaaaaatcg atggttatcc attatttatc gaaaatgcac tccctggaga agaagtagaa
    14401 atactagtag taaaagcagg caataagttt ggatatggaa aagtcgaaac atttattaca
    14461 caatcacctg atcgtcagcc tgtagaaaac aatgcattac ttcgcatagg tatcgcacct
    14521 ttagcccatt tgaagtatga acaacagctt gtcttcaaac aaaaacaagt agagcatgta
    14581 atggcgaaga ttgctaagat gccagaagtt cctgtacttc caacggtcgg aatggcagaa
    14641 ccattcggct accgtaataa agcacagatc cctgttcgcc gtatcgatgg acagttagcc
    14701 accggttttt acaaaaagaa tagccatgat ctagtcccta tcgaagattt ttatatccaa
    14761 gatcctgtca tcgatcaagc aatcaaagta gtcagagata tcttgcaacg gttccaagta
    14821 cgaggatata atgaagaaaa aaatgaagga cagcttcgtc acatcattat ccgtcgtggt
    14881 cattattccc atgaaatgat ggtcgtttta gttactcgta aagaaaaatt ctttaaatcc
    14941 aaggaaattg ctgaagctat tcatgaagaa ttgccagaag tggtttcggt catccaaaat
    15001 atcaatgaag aaaaaacaaa tgtgatttta ggagacaaag aaaaagtttt atatggcaga
    15061 gactatatcg aagatcagct attaggaaaa acctatcgaa tctcagcaaa gtcattttat
    15121 caagtaaata ctcaacaagc agagtatctg taccaaacag ccatcgagtt tgccgagtta
    15181 gataaaaaag acattgtgat cgacgcttat tcaggaatag gtacgattgg cttgtcttta
    15241 gcagacagag tggataaggt gtatgggatg gaagttattc cagaagcaat tgaagatgca
    15301 cagttcaatg ctttaaataa taaaatcgaa aatgttcatt atgaagtcgg aaaagcagaa
    15361 aaagttatga aaaaatggca agaaaaaggg atcaaaccat ctgtgatcgt agttgaccct
    15421 ccacgaaaag gattagatca tcattttatt gaatcagcaa tcgaaatgtc accagaacga
    15481 atcgtctata tctcatgtaa tccggcaact tttgcaagag atgctaagtt atttgcagaa
    15541 gcaggttatg ccgtgaagaa agttcagcct gtagatcttt tcccgcaaac ccatcatatc
    15601 gaattagtcg cttcattcgt taaaggagaa taaaaatgga ccaaaaagaa aaaatgaaag
    15661 aattgatcaa aaagaaacaa ggtggaggcc gatccaaaca aatggaaaca cctaagaatg
    15721 atcgtaaaaa catgagaaaa ggccctaaaa tctttaataa atagacgtgc taaagaaaga
    15781 ctaagaagtg ttaacttttg ttattgtttc ttagtctttt t
//
LOCUS       DAZUMV010000054        15675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000054 DAZUMV010000000
VERSION     DAZUMV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.054"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..583
                     /locus_tag="ACG8FK_001537"
     CDS             17..583
                     /locus_tag="ACG8FK_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5097149.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            728..815
                     /locus_tag="ACG8FK_001538"
     tRNA            728..815
                     /locus_tag="ACG8FK_001538"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:760..762,aa:Ser,seq:cga)
     gene            1151..2125
                     /gene="bsh"
                     /locus_tag="ACG8FK_001539"
     CDS             1151..2125
                     /gene="bsh"
                     /locus_tag="ACG8FK_001539"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS5097150.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            2450..4345
                     /gene="asnB"
                     /locus_tag="ACG8FK_001540"
     CDS             2450..4345
                     /gene="asnB"
                     /locus_tag="ACG8FK_001540"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS5097151.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(4540..4986)
                     /locus_tag="ACG8FK_001541"
     CDS             complement(4540..4986)
                     /locus_tag="ACG8FK_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS5097152.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            5214..5363
                     /gene="rpmG"
                     /locus_tag="ACG8FK_001542"
     CDS             5214..5363
                     /gene="rpmG"
                     /locus_tag="ACG8FK_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5097153.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            5451..5969
                     /locus_tag="ACG8FK_001543"
     CDS             5451..5969
                     /locus_tag="ACG8FK_001543"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5097154.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(6046..7002)
                     /locus_tag="ACG8FK_001544"
     CDS             complement(6046..7002)
                     /locus_tag="ACG8FK_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5097155.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            7168..7974
                     /locus_tag="ACG8FK_001545"
     CDS             7168..7974
                     /locus_tag="ACG8FK_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097156.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            7971..8960
                     /locus_tag="ACG8FK_001546"
     CDS             7971..8960
                     /locus_tag="ACG8FK_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5097157.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            8957..9961
                     /locus_tag="ACG8FK_001547"
     CDS             8957..9961
                     /locus_tag="ACG8FK_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5097158.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            10093..10635
                     /locus_tag="ACG8FK_001548"
     CDS             10093..10635
                     /locus_tag="ACG8FK_001548"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS5097159.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            10655..11353
                     /locus_tag="ACG8FK_001549"
     CDS             10655..11353
                     /locus_tag="ACG8FK_001549"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS5097160.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            11374..11586
                     /locus_tag="ACG8FK_001550"
     CDS             11374..11586
                     /locus_tag="ACG8FK_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS5097161.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            11586..12548
                     /locus_tag="ACG8FK_001551"
     CDS             11586..12548
                     /locus_tag="ACG8FK_001551"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS5097162.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            12566..12961
                     /locus_tag="ACG8FK_001552"
     CDS             12566..12961
                     /locus_tag="ACG8FK_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5097163.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            13124..13489
                     /locus_tag="ACG8FK_001553"
     CDS             13124..13489
                     /locus_tag="ACG8FK_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS5097164.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            13486..15297
                     /locus_tag="ACG8FK_001554"
     CDS             13486..15297
                     /locus_tag="ACG8FK_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS5097165.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(15359..15526)
                     /locus_tag="ACG8FK_001555"
     CDS             complement(15359..15526)
                     /locus_tag="ACG8FK_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS5097166.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         4868 a         2636 c         3276 g         4895 t
ORIGIN      
        1 aagtggggga gttttaatga gaagaaaagt ctataccaag gatcaaattc taaaagctgc
       61 ttatgatgtt gttgcaaagg aagggtttaa aggatttact gcgagaaata ttgctaaaaa
      121 gatggggatt tcgacacagc cgatttatct ggaattcaaa aacatggaag atttaaaaaa
      181 tacgttgctg gattctatct ttgaacaact aaaagaagat gttttcccta ttgaacgtac
      241 tggtgataaa atcattgatc tcggtttgaa ctatatctac tttgcaaaaa agcatcatag
      301 tctttacatg gcattgtaca ttgatgaaca tggggacgga aagaaaatgc atgatttttc
      361 atacaattat ttccgtgaaa tagttaaaga agatgaaaga tacaatcaat tatcggatga
      421 atatgtcgat gctctgcata ctggtacatg ggtagcagtg actggcttag cgtcattgat
      481 gtcatcaaac atcatgcatc caacagaaga acaaatcatt gcactgatca aacggacaat
      541 tgactcgatt ttagcattag ataatcctga aataatcaga tagtgttcta tcagtatgaa
      601 tttgatcggt aagtccgttg gctgcaaact gtcaatggac ttttttgcta aaaaaagcga
      661 tttctgttat ttatctatta gaaataggct tgacatttcg tgaaaaaagt ttaaaaatag
      721 tacttacgga aggttggcag agtggtaatg caccggactc gaaatccggc gaaccggcta
      781 ataccggcgc gcaggttcaa atcctgtacc ttccttatat caacgattag aggttgtgaa
      841 gaagctgtcc tgcatcaagt aataagaacg gccaaacaaa aatagctttt cctatttttt
      901 cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttgt tcggaataag
      961 gggctgtgac aagacttttg tcacagcccc ttattgttgt acttttaacc tacaatattt
     1021 tttgattccc cctgagtact attttttata aatgctagta aatcaatctt ctttggcctt
     1081 ttttttgata aaaaaaaata ctagatgtaa gataaaagtg taaaaacgat ttgttttagg
     1141 agggaaaacg atgtgtaccg caatcactta tgcaacgaaa gaccattatt ttggaaggaa
     1201 ttttgattat gagatgtctt ataatgaagt agtcactatt actccaagaa attatcgttt
     1261 cgattttaga aaggtaaaaa atttagataa acattatgca atgataggaa tagcagctgg
     1321 ggtatccaac tatccactct attatgaggc tacaaatgaa aaagggttga gtatggcagg
     1381 actgaatttt cctggcaatg ctgattataa agaattacaa gaaggaaaag ataacgttgc
     1441 accatttgag tttattccat ggatactagg gcaatgctca accatagacg aagctaaaga
     1501 attattggct actatcaatc tagtcaatat cgattttagt gaaaaactac ccttgtcacc
     1561 tttacactgg ttattagctg ataaagaaaa atcgatagta attgaaagta tgaaagatgg
     1621 acttcattta tatgataatc ctgtcggtgt gttgacaaat aatccgccat ttgattatca
     1681 attatttaat ctgaacaatt atcgctcgct atcaaatgga acaccagaaa atcatttttc
     1741 aaaccagatc agcttgaatg tttatagtcg tgggatggga ggattgggct tacctggaga
     1801 tctctcttct gtttctcgat ttgtaaaagc cacatttact aaaatgaatg ctgcatcagg
     1861 tgattcagaa tctgaaagta tcagtcaatt ttttcatata ctaggctcag tcgagcaaca
     1921 aaaaggagta tgcgatactg gagaaggaaa atacgaatat acgatttatt cttcttgctg
     1981 taacgttgat aaagggattt attattatcg tacgtatgaa gacagtcaaa ttactgctgt
     2041 aaatataaat aaagaagact tggatcgtca tgaattggtc agttatccag tcataaaaga
     2101 gcaacagata aactatgtca attaattaat gaatagatcc gtttggtatc atattttctg
     2161 atacagtcat aattagacac gatctttagg tcgtgtcttt ttttgctgtt ttttttggaa
     2221 atagcatcca ctaatatttt tttaacacga agagaataag gtagcctcaa aaaagaaaac
     2281 atggtaaatt agatattggg actttatttt ctgtaaaaac agaaactttc atcttttgtt
     2341 ttcacgtgac gaaattgtaa gagagaaaat attcgtccgc tatttattct atgataagat
     2401 gcatatgaat gaaataaaca gtaattttat attaggatgt gaaagttaaa tgtgcggtat
     2461 tgtaggtttt gttaacgaca aggacaacaa aaaaacaatt attaatacaa tgatggatcg
     2521 aatcgttcac cgtggaccta atagttcagg agaatatatc gataaacatg ttgctttagg
     2581 attcagaaga ttaagtatta ttgacttaga gggcggtact cagcctattt acaatgaaga
     2641 tcggacaaag atcattattt ttaatggcga aatctataat taccagcctt tgagagaaga
     2701 attgattgca gctggccacg tttttcaaac acatgcagat acagaagtcc ttttgcatgg
     2761 ttacgaagaa tggggaacag aattattgca aaagatccgc ggaatgtttg cttttgctat
     2821 ttgggataat gaaaagaatg agttattcgg tgcaagagac cactttggga tcaaaccata
     2881 ttattatgca gaaatgaacg gaacatttat gtttggttca gaaatcaaaa gttttctgcc
     2941 tcatcctgat tttaacaaag aattgaacaa agaagcgttg aaaccttata tgactttcca
     3001 atattctcca ttaaacggcg aaacattctt caaagatgtc tatcgtttgc ctgaaggcca
     3061 ttattatacg tataaagatg gaaaactaga cattcagcaa tattgggatg cagattttga
     3121 aacaaaagaa acgcattccc gacaagaatg gatcgaaaaa atcgacgaaa ccgttcaagc
     3181 ttctattgaa gcacatacgg tcagtgatgt agaagttggt tctttcttat ccagcggggt
     3241 tgattccagt tatgtaacct ctgtattgaa accagatcat tctttctcaa tcgggtttga
     3301 tgataaaacc tataatgaag cgattgaagc cagaaaacta accgaactat tggacttaga
     3361 taatacagca gcagtgatcg acggagacat gtcgttcaaa gcattccctt tgatccagta
     3421 tcacttagat gaacctgatt caaatccttc ttgcgtacct ttgtactttt tagcaaatct
     3481 tgcttcacaa agtgtgcggg tagtccaatc tggagaaggg gcggatgaac tgtttgcggg
     3541 ataccaaacg tatggattcc atacgaattc aaaattcatc cgtgtgattg ctcaaggatt
     3601 gaaaaaatta ccaaaaggaa cacgctataa tttaggacgt aaaatcggaa aaatgaaaaa
     3661 tttccatgga cggatccatt tatatgaatc acttgctcct gcaaaagaat attttatcgg
     3721 tcatgctcga gtgttcgaag aatcagaagc atcagaagtg ttaacaccaa aatatcaaac
     3781 agcaccatct gtggatgaaa tcatgactgt tcattacgag aaaacagaag gaatcaaaga
     3841 tgaagtcaat aagatgcaat atgtcgatct tcatcaatgg atgccaaaag atatcttgtt
     3901 aaaggcagat aaactttcta tggcaagttc tttggaagtc cgagtacctt tattggatat
     3961 cgaagtcatg aaattggctc aacaaatacc aagtaagttt ttattgaatc aaaacaacac
     4021 aaaagatatt ttcagacaag ctgcgaacaa acatcttccg gaagaatggt cgaatcgtgt
     4081 gaaattagga ttccctgtgc caatcaaagc ttggttgaaa gaagaacacg gctacgaaca
     4141 agtcaaagca ttattcgaag cagattttgc aaaagaattt tttgatcaag aaaaaatcat
     4201 gaagttgcta gatgaccatc acgaaggacg taaagaagag caaagaaaaa tctggacaat
     4261 cttcagtttc ttgacatggt ataaagtcta ctttgtagat gaaacgattc cgcaagcaga
     4321 agcaatcgac tatgtgactg tttgatgaaa atcaaatatc aataaaaaag aggttttctc
     4381 ctaccgttta acactgagtg ataagaaaca atttcaaaaa acagctcttc ccgttttata
     4441 gatattatga cttatggttc ggagttggca gaggaacacc attgtttcga acataagggg
     4501 gttgtgacac gattttcgtc acaacccctt tacatatgtt caataatcga tttctttttc
     4561 ttttgtccca tcttttttat aaatgaacaa agttgattct ttgttttgtg ctacttgctt
     4621 tcctttttcg attgcttctt tttttgtatc aaaatggttg cttgcctttt ttgccccttt
     4681 agatttcacg atccattgtt cttcctcata ttctacgatc acatctgaat caagcagttt
     4741 tccagcacga ggattcgtat cgtgttcatc cgtcttgctt ggattttttt ctttttcgaa
     4801 agttcgtttt tctgattcac tagcatcttc ataccatttt tttgcttgat caatggcaat
     4861 tgggatcgca cggttatcag ggtacccttc atctaataat gcatttgcga tgtcgattgc
     4921 tttttttctt gtcaatttat ctaagttttt cattgatgca ggaaagtcct tcatgttcca
     4981 tggcatatta gtatccccct ttattaaaaa acataatcta tataaacaag tgtaagggta
     5041 tttatacaaa aagaaaaaga ttttgtttga gaagtaaaac cgttgtattt atcgtcaagt
     5101 agttttactt aacaaaatcg tgaaaaacac tagcaatcta gaaaaaatca tggtaagata
     5161 atcaagtctg agagtcatct tagaggattc ttgtaaaaat gggagggaaa aacatgcgcg
     5221 taaacatcac tttagagtgt acttcttgta aagaacgtaa ctatctaaca agcaaaaaca
     5281 aacgtaacaa tcctgatcgt ttggaaaaac aaaaatattg cccacgcgaa cgcaaagtta
     5341 ctttgcaccg tgaaacaaaa taatgttttt aaagttctca attccttgct ggtcaaagaa
     5401 ttgagacttt ttttatctca aaacatctaa aatcataagg agcggataat atgtttattt
     5461 ccatgtgggc acaagataaa aatggtctga tcggcaaaaa tggtttgtta ccatggcgat
     5521 tgccaaatga tatgcgtttc tttcgcgaac atacaatgga tcgaatttta gtgatgggaa
     5581 gaaagaccta tgaaggaatg ggggatcttt cacttcctta tcgccatatc atcgtgttga
     5641 caacgcaacc aaattttcgc acgaaagaaa atgcggaagt aatgcattcg atcgatgaac
     5701 ttttgtcgta tagtcaaacg atctcagaag atatctatgt ttcgggtggt agtcggattt
     5761 ttcaagaact tcttccttac actggtcgga tctggcgaac attgatcgat agcacgtttg
     5821 aaggcgatac gtatattggg aatattgatt tttcagactt cgcccttata gaggagtatg
     5881 aggggataac ggatcaagaa aatctatatg cccatcgttt tcaaaaatgg gaaagagtaa
     5941 aaaatagggt tgcttcaaaa gaagaataaa atctatgcgt ctagtttgtt aataagaaaa
     6001 aaagcatctc acgactaatc gtgaagattc tttttttagt taaaatcaat ttagtttttc
     6061 tttgatatca ttgaccattt cctgactggc aataggtccg ttatatagcc aactagattc
     6121 aggtccgaat tgcagtacat gattgtctgt cactgcttta agattcttcc agcttggttc
     6181 attgaacatg gcagcatctt tgtcactgtt cacaaggatg atgtagtcag catctaattc
     6241 agctagcttt tcagaggaaa cagcagacca gtcagaagtg gcttttttgg agacctcttc
     6301 cactagatca ggaacaccaa attccagatc gtcgtatacg attcttccac ttgaccgatt
     6361 tttgctgacc ataaaggctg agttgttggt cacccacaac actgcagctg tcttgttctc
     6421 gattttatct gctagttctt ctcgagtttt cttgatggat ttttgataat cggaaatgat
     6481 tttttctgcc tctttttctt ttgccaaagc tttgccgaca tcttttagtt gttcttccca
     6541 tgtgacatct gttccgtttt ttacaacata agttggcgcg attttgctat attgttcgta
     6601 tttcccgcct tcgacagtag cggaagaaga aatcaacagc aaatcaggct caaagcttaa
     6661 cactttctca taaggaaggt cataagaaat cgttgggaca tcctttaaat catcttgcag
     6721 ataatcttga atggaaccac tgccaacagt ccactgggcg ataggttttt cccctagtgc
     6781 aatcagatag tcttctaagt aactgccgat gatccgtttg ggcgcgtttg gaatctctac
     6841 tttatgtcca agtgtatctg tgactgtatg agtactattg gatgaagtcg tcgaattggc
     6901 agttgtagct gtttctttat ctgaagaggg ggagcaagcg ctcagtactc cagccagcag
     6961 caatatgcta gataatgtga atacttttgt ttttaatttc atttctttcc tcctaacata
     7021 attgagaatc tttctcattg gttattattc ctgatatttc gaacgctgtc aaccgcttca
     7081 tttggctata gatatgaaag tagcgcttga cgaaatgttg aaacagcgtc ataattgaga
     7141 atgattttca gcgaaaggat gggtgaaatg acgagattaa gagcagctga cattgcagcc
     7201 ggctacgaaa acaaaaaaat cattgaaaac atgtctgtta cgattcctga agggaaaatc
     7261 actagtttga ttgggccaaa tggaagtgga aaatctactt tattgaaaag ttttacccgt
     7321 atactacctg tgaaatcagg aaagatcatt gtcaataact ctgatatctc tacatacagt
     7381 tcaaaagaac ttgcaaaaaa aattacatta ttagcacaaa ctagtgaaca ccctacaggt
     7441 atgacagtag aagaagtagt atcttacgga cgataccctt atcaaaaact gttcagtggc
     7501 atggacacgc aagatcatga agcaatcgag tgggcgttgg atgctacagg tctagctgga
     7561 tttcgtatgc gggatatcgc aacgctgtct ggtggacaaa cccagcgtgt ctggatcgct
     7621 atggcgcttg ctcaggaaac agatatcttg atcttggatg aaccgacaac atttttagat
     7681 ccggcacacc aattagagat cctacagtta ttgactgaaa tcaatcaaat gaaacaaaca
     7741 acgatcttga tgtcgattca tgacattaat catgcttcac gtttttcaga ttatttatta
     7801 gcaatgaaaa acggtaagtt gatcacagaa ggtacacctg aagaagtgat cacaaaagag
     7861 tgtatgaaaa aaatatttga gattgatgca acgattgtca cgcttccaag aacaaataaa
     7921 ccagtttttc taacctatga tttgttgcca aaagagcagg aggatcaatt atgaggacta
     7981 tgaagaaaaa tagactcctg cttttggtct gttttgttct tttgattatt ttttttgttg
     8041 ctctttcaaa tggcgcagcg actgtgggaa aagcagattt ttggcaggcg ttgtttgatt
     8101 ttgatagatc aaaacaaagt caccaaatca tacgaaatat tcgtttgcct cgtgtggcgg
     8161 gagcatttct tgtagggagt tgttttgcat tgagcggtgc attgatgcaa ggagtgacaa
     8221 gaaatccttt agcagattca ggcttactag gaatcaacgc gggtgcttcg ttggcaatgg
     8281 cccttagttt tgctttttgg cctaaagctt ctgatttttc tgtgtttcta atgtcattgg
     8341 caggttcttt tgctgtaact ttgctcgtat ttggtttttt gcagttttcg aaaatgggca
     8401 tgaatccttt acagctgatt ttagctggcg tagctattag ttcttttttt actgctatca
     8461 gccaagcact tacccaattc tttaatctgc agcaggatct tgctttttgg tttgtaggcg
     8521 gagctgccaa tatcacatgg aaacaattag cagtattgtt tcctttatat atacttgctg
     8581 ttttggtttc tttttcatta ggaaaagcaa tatctttgat tcatttgagt gaaacacatg
     8641 cattagctct tggaagtcat cctaaacgaa cgcggttgat tgttttctgt ctagttgcag
     8701 tgttagcagc cattgccgtt tcgttagtcg gacctgtttc atttattggg ctgttggttc
     8761 cgccagttgt tcgcggaatg gttggaacgg actatcgtta tgttctacct gtctcttttt
     8821 tagttggcgg tggattggtt atgttggcgg attttacagc gaggatgatc aatccccctt
     8881 ttgaaacacc ttttggatta gtcattgcat tgattggcgt tccgttttta ctgatacaag
     8941 taaggaggga acaacggtga aaaaaatata ttggacgttc tatttcttgc tttttgtact
     9001 tgtcatcctg tctataggta gtttaatgat tggtacgcct tttatttacc tggatcagct
     9061 ttttgccacg ttcataggga aagaatcatc ttctttggcg ctgattgtta gagaatttcg
     9121 agttcctcga ttactgatca gtttattagc aggagcttgt cttggcgttt ctggttatat
     9181 cttgcaagga gtaacaagaa atgaattggc agattctagt atattgggca ttaatgccgg
     9241 agcaggattt ttagtgatgc tttatttagg tttcttttcg cactattcgc tgccgtttct
     9301 attgctagtt gtagcctttc taggtggtgt tttagctgct tgctgtgtct atcttgcggc
     9361 ctatgatcga aatggctttt tgggtatgaa tcgaatctta ttgtccggta ttgccgttaa
     9421 tgccggcctt tctgcattaa cattgctatg cacgatccaa ttatctaagg aaaattatgg
     9481 atttgtgaat gcatggttag cgggatcaat ctggggtgcg agctggcctt atgtttgggc
     9541 attgcttcca tgggcggtta tcttaattcc gctaggagga ctttatgcac agaggattgg
     9601 attgctatca tttggtcaag aacgcgcgca aggattaggc cttaacgtgg ctaaaagtca
     9661 aaaaatcctt ttgttgttag ctgttgcttt ggcaagcggt agtgtagcgg tgacaggtag
     9721 tttatctttt gtaggactac tagctcctca tgctgcgaag ctagtcgtac gtaaagaaaa
     9781 taactggacc tttgttctga gcggtttgtt cggttccatt tttctgctta gtgcagatat
     9841 ggttgctaga gtgattctac caagtgggga agtacctaca ggaattttga ttgccctttt
     9901 tggtgcgcca tattttctgt atgcactttt tatcagacaa aaacacgcgc tgaacagttg
     9961 aaatttatta aaaaaggaag gccaatcatt tctaggtctt ctttttattc tatttatgca
    10021 tttatttgaa agttcgattg ctatttttct ttttttccta taccatttta tgagagaagg
    10081 aagggtgagt ttttggacca attgaaagta agattacgcc ggaaaggaat cgaaaagctg
    10141 aaaaatctgg ccaaaagtgg caagaaaaag gaaaaagaac agcgaatcta taaacagcta
    10201 ttcgccagcg agtactggaa gtcagcaaaa gtgataggga cgactttatc gaatgaattt
    10261 gaactggata cacagccggt tatccaacga gcagaaaaag aacaaaagaa aatagttgta
    10321 cctaaaactt tgccgaaacg tcagatggcg ttttatgaaa tggatgaaca gtccgttctt
    10381 gtacgttcga gttttggtgt tcttgagcca caatcagaga agctctattt gccggagatg
    10441 atcgatctcc tcattgtacc aggtattatc tacaatagag ccggttatcg aatcggtttt
    10501 ggcggtggtt attacgaccg gtatttaaat aattttcata aaaaaacatg tagccttgtt
    10561 tttcgtgaac aattgaatga acaatggcaa ccggagagct ttgatcagaa gatccaacaa
    10621 ttgtttattg actaaaatga aatgggaggt tttcatgaat tatcaacagc aaataaaatt
    10681 gcgtgcatgg ttaagacgac cgttatggac ctatgctttt ttagggatac agacagcggt
    10741 cttcctcatg atggaattat ttccgcgact tgagatccct tattatgccg gaatgtatgg
    10801 tccttatctt gttcacttca atgaatggtg gcgtttagtg acaccaatct tcatccattt
    10861 tggactgatg catttcgtaa tgaactcact gatactgtat tttatggggc agcaaatcga
    10921 ggcaatctat ggccattggc gttttttctt gatttatatg ttcagcggga taatgggaaa
    10981 tacagcaagc tttgctttta acgaagcgaa tgttctgtct ggtggtgcga gtacttcgat
    11041 attcggtttg ttcggtgcgt tgtttatctt agggtttcat tttaaacata acccagcgat
    11101 ccagcagcta gtcagacatt ttcttttatt tattgtgttg acatttgttt ttggtcttct
    11161 ggatacctct gtagatgttt ggggacatgt cggtgggcta attggcggat tagtgttagg
    11221 gaatgttttg ggtctaccga aacaacagac aagctattcg atacaccaaa ggattctttc
    11281 tacactagtt tttgtatttc tttttgtaat atgtatttta ctgggtttaa aaaaatatgg
    11341 tttacttgta taatatgaag gaagtgctta aagatggaga gcttatatga tgtacagcag
    11401 ctgttcaaaa aatttggcat ctttatctat gtgggtgcac gcatctatga cattgaatta
    11461 atgactatcg aattgcgtaa tctgtatgat agccgtttga ttgatcatga tacatacatg
    11521 acagcgtggc gcatattaaa aagagagcac cgaattgaag aaagccgaaa gaaaggaaat
    11581 ttgtaatgga caagaagatt attgggattg atttaggtgg aacaacagca aaatttgcaa
    11641 ttttaacacc agaaggagag attcaacaaa aatggagcat cgataccaat attttggatg
    11701 atggtaagca tatcgttcct gaaatcattg aatcaatcaa ccatcgtttg aatttatata
    11761 atatgaaagc cgaagacttt atcggaatcg gcatgggtac cccaggaagc gtggacagcg
    11821 aagctggaac ggttattggt gcgtataact taaactggac agaagttcaa tttgtgaaaa
    11881 aactgatcga ggcaggaaca ggaatcaaat tcgctatcga taatgatgcg aatgttgctg
    11941 ctttaggtga acgatggaaa ggtgctggag aaaacgatcc agacgttgta ttcgttactc
    12001 taggaacagg tgttggcggt ggtatcgttg ctggcggaaa tttgattcac ggcgtgagcg
    12061 gagcaggcgg agagatcggc catatcactg tggatcctga agggttcgaa tgtacttgcg
    12121 gtaaacgcgg atgtttagaa actgtgtcta gcgctactgg tgtggtacgt ctaggtcgtt
    12181 acttggcaga agaatacgca ggagattcaa aactgaaagc aatgttagac aacggtgaag
    12241 aagtaacaag taaagatatc tttgaaatgg ctcaagaaga cgatccgttt gcattgatgg
    12301 ttgttgatcg cgtatgcttc tacttaggtt tagcttgcgc caacttgggg aatacattga
    12361 atccgtcaag tattgtgtta ggtggaggag tatcggcagc aggagaattt ttacgtagcc
    12421 gtgtagagaa atattttaaa gaatttactt tcccgcaagt aagagaatca acaaaaatca
    12481 aattagcaga gcttggtaat gaagctggtg tcattggtgc tgcctcatta gccttgaaat
    12541 ttgcttaaaa aaacggaggc attacatggt tttatgggta attaatatca ttttgctatt
    12601 gattgtgctt gcaatcatct tttgggaagt ctatctacga gtaatggcca agcgttcagc
    12661 aacgactttg acagaagaag aatttcgaga aggaatgaga aaagcacaag taatcgatgt
    12721 tcgcgaaaaa gatgtgtttg atgctgggca tattttaggt gcaagaaaca tcccttacac
    12781 tgttttgaaa gattcattag gatctattcg aaaagaccag cctgtctata tttacgatca
    12841 aaagaaaagc ttgagcatcc gtacagcaaa taaattacgt aaagcaggtt atcaagagat
    12901 ttatttactt aagggtggat atgacggatg gtctggaaaa atcaaaagta aaaaagtata
    12961 aaacagatga ttgtatgaca gctttaaaaa ccgaatcaat cattagattt tgaccaagat
    13021 cacatgaacg gcgcaatgga tagttgtgcc gttcattttt tttattttgt gctattatct
    13081 acatatataa gctaagcgaa aaggcagaaa gaaggaaaca actatgatca aattaaaacg
    13141 tgtttacctt gatgctgaaa aagaggatgg ctatagaata ctcgtggata ggttctggcc
    13201 acgaggaatt tcaaaagaaa aagcgcagtt agatctgtgg ttgaaagaaa ttgcacctag
    13261 taatgaagca cgcaaaactt ttgaccatgt accagaacga tttgcttcat ttgcaaagaa
    13321 ataccagcaa gaattgattt tcgaagaaga aaagaagcaa gctattttta aactcagtca
    13381 acttatgaaa gaacatccag tcattacttt tgtttatggt gcaaaaaatg aacatcagaa
    13441 ccatgctgtt ttattgaaaa aatttatcga aatggaggca tgcaaatgaa aatagggatc
    13501 attggtgccg gacctagagg attaagtata gcagaaagga ttctccataa taatcaggac
    13561 agacattctc ttgaactttt tcttttcgat cccaatggtc ctggaggtcg agtttggcgg
    13621 ttgaatcagc caccggagtt attaatgaat tctgtttctc aacaagtgac gctttttaca
    13681 gatgaaacat tgtccagtgg aggattggta acacagggac ccaacttgtt tcaatggagt
    13741 aaaggacctg ctacagatta tatcgaacag acggtaacca aaaataagca actaattata
    13801 gaagaaatca atcgtctgga aaaagatcag caatctactc gatgtttata cggactctat
    13861 caacgctggt ttttctcaaa gttgcaagaa gaatttccag cttctattca gtttgtttat
    13921 tctttagtaa aaaaagtcag caaaagaaaa gatggttatc ttttacagac agatcagcaa
    13981 agctattcta tcgatcagct tgttttagca agcggtcact gggaaaatga tgagacagaa
    14041 gaagaaagag tgtgggtaga ctacgcaaag aaaaatgatt tactgtatca gcttccttct
    14101 aatcctgcag atgtcgatct ttcgaagata ccgcctaaag aaaatgtctt tttaagaggt
    14161 ctaggcttaa gtttttttga ttatgtcgga ttgtttacag tatcaagaga agggcgcttc
    14221 gagaacgaaa aaggaaaact tgtctatcat ccatcaggca aggaaccgat tgtatacagt
    14281 ggatcaagaa gggggctccc ttattaccct cgaggaagaa accaaaagca aggcggatca
    14341 atggcatggc cacggttact aacaaaagaa aacctggagc aatggcatca gtcgggattt
    14401 ttgactggcg aaacattttt tgactatctg aaaaaagacg ttgagttgtt ttattacaag
    14461 aaaatcatcg aagaatatca tttgccgatt ccaaagaaaa attttgagca tgattttctc
    14521 tccctctctt cagaggagtg tctcggaaag catccagcac taatccctta tagatgggat
    14581 tggtcgtatt tagaaattcc tcttacctat caggatgagt cctttgcaga agctagccga
    14641 gcatttattg aagggcagat aaaagaagcg gaaaaaggca attgcacagg agcatttgct
    14701 tcaacatttg atgcattaaa agactggcgt gatccaatcc ggcaggctat tgaatggcac
    14761 gttttttctg caaaggaata caaagaaata ctatggggat ggtttactca tctaaatagc
    14821 tttttaacga tcggtccgcc agtcattcgg acgaaagaat tagcagcact gatagatgcg
    14881 ggtatttttc atttgttcga accgccattt gatattcagc aaaagcacaa ccgctatgtt
    14941 atttctctac aagataaaga aatcagcagt cgctatttga ttgaagctcg attgccacaa
    15001 acaagctttt ctcgttcaag taatcctgtc ataaaaaatt tgaaggaaaa caaacttgtt
    15061 cgcccttttt cattttcaga taatacagga agttatcatt cagaagctat agatgttgag
    15121 cgaggtacga atcgcgtgat tgatgcttca ggggaaatcc agtctgactt ttattgctat
    15181 ggaatacctg ttgaaggtgt ggattggcta actgcagcag tagctagacc ttatacagat
    15241 ccatggaatc tgagacaggc agataaaata gcacaactta tattgactga caaataaaaa
    15301 tacgcatgat gacttatctg ttctttcaca agtcttcatg cgtgttttta aataattatt
    15361 aacgagaaac gcgtttgcgc attgcttttt tacggttttc ttcgatcatt gcttcttttt
    15421 cgtcaattgg atcgatcact gtttttttca cagaagcgac caaaccagca acggcagcgg
    15481 ctgttgccag acttcctact aatattccag atacaaattt tttcattgaa tccacctcat
    15541 ttattatttg ttatatctat tatggaacaa aagagaaaaa aaggctagtt ttacgtttgt
    15601 ttgtaaacct ttgcaaaaaa tccttgaaaa tgtttactaa atgttttatt ataaatttac
    15661 catatgaatt tacga
//
LOCUS       DAZUMV010000055        15593 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000055 DAZUMV010000000
VERSION     DAZUMV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.055"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..156)
                     /locus_tag="ACG8FK_001556"
                     /pseudo
     CDS             complement(43..156)
                     /locus_tag="ACG8FK_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(539..1771)
                     /locus_tag="ACG8FK_001557"
     CDS             complement(539..1771)
                     /locus_tag="ACG8FK_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS5097167.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2028..2597
                     /locus_tag="ACG8FK_001558"
     CDS             2028..2597
                     /locus_tag="ACG8FK_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5097168.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2775..3215)
                     /locus_tag="ACG8FK_001559"
     CDS             complement(2775..3215)
                     /locus_tag="ACG8FK_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS5097169.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3373..4137
                     /gene="map"
                     /locus_tag="ACG8FK_001560"
     CDS             3373..4137
                     /gene="map"
                     /locus_tag="ACG8FK_001560"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS5097170.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4169..5092
                     /locus_tag="ACG8FK_001561"
     CDS             4169..5092
                     /locus_tag="ACG8FK_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS5097171.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5168..6307
                     /locus_tag="ACG8FK_001562"
     CDS             5168..6307
                     /locus_tag="ACG8FK_001562"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS5097172.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6300..7100
                     /locus_tag="ACG8FK_001563"
     CDS             6300..7100
                     /locus_tag="ACG8FK_001563"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5097173.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7100..7927
                     /locus_tag="ACG8FK_001564"
     CDS             7100..7927
                     /locus_tag="ACG8FK_001564"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5097174.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7932..8639
                     /locus_tag="ACG8FK_001565"
     CDS             7932..8639
                     /locus_tag="ACG8FK_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5097175.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8739..9605
                     /gene="rfbA"
                     /locus_tag="ACG8FK_001566"
     CDS             8739..9605
                     /gene="rfbA"
                     /locus_tag="ACG8FK_001566"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS5097176.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9619..10191
                     /gene="rfbC"
                     /locus_tag="ACG8FK_001567"
     CDS             9619..10191
                     /gene="rfbC"
                     /locus_tag="ACG8FK_001567"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS5097177.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10213..11241
                     /gene="rfbB"
                     /locus_tag="ACG8FK_001568"
     CDS             10213..11241
                     /gene="rfbB"
                     /locus_tag="ACG8FK_001568"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS5097178.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11339..12190
                     /gene="rfbD"
                     /locus_tag="ACG8FK_001569"
     CDS             11339..12190
                     /gene="rfbD"
                     /locus_tag="ACG8FK_001569"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS5097179.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12224..14257
                     /locus_tag="ACG8FK_001570"
     CDS             12224..14257
                     /locus_tag="ACG8FK_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097180.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14301..15581
                     /locus_tag="ACG8FK_001571"
     CDS             14301..15581
                     /locus_tag="ACG8FK_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS5097181.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         4765 a         2693 c         3246 g         4889 t
ORIGIN      
        1 aaataacccc tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt
       61 ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag
      121 taaaatagat gcatgcgtat gtcttagtcc ctcaataaac agtcgaagag aaaataggca
      181 aaaattgatt actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca
      241 acagattcga attaaacgat ttcgattggc ttataccagt cctatccgac gaaagatata
      301 tcgttcttaa ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg
      361 gagatgactt tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt
      421 tcaaagtacc atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat
      481 agcttagatc ctttattcta tgctttatat tattttttgc atgtgtgcga aagtatactc
      541 aagcccaatc gccatttcgg aaaatcggta ctgtagagcc atcttcacgg attccgtcga
      601 tatccatctt gtcagatcct accatgaaat ctacgtgggt ttgacttcta ttcaatccag
      661 cttctgctaa ttcttcatcg gacatttctg taccgccttt cacactgaag gcataagcag
      721 aacctagtgc taagtgattc gatgcgtttt catcaaataa tgtattgaaa aagatgatgc
      781 cagattgaga aataggtgaa gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac
      841 cttcatctgt atcaagcagt tttgctaaga catcttctcc ttgttcggca gaaaaatcaa
      901 tgacttttcc atctttaaaa gtaaatttca tacccgagat gatggtgcca gcataactca
      961 gtggctttgt acttgaaatg taaccatcta ctcgatgact gtcaggtgct gtgaacactt
     1021 cttccgtcgg catatttgcc atgaattttt ctccacgagc attatagctt ccagcgcctt
     1081 cccaaagatg gtttttaggc aatccgataa tgatatctgt accgggagca gtatagtgca
     1141 gggcagaaaa ttgttctcga ttcaattctt ctgctttttt tgctaatttt tcatcatgct
     1201 tcttccatgc taagacaggg tcttcttcat agacacgagt cgttttgaaa atctgatccc
     1261 ataatgcctc aacttgtttt tcttctggca gttctgggaa gacttttgct gcccattgtt
     1321 taccagccgc agccacgaca gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat
     1381 tcattagtgc tttgccagcc gctgattgat aagacgctac tcgatggcta tctacaccag
     1441 caaatgcatc gggatcggca gaaacaacac tgatcctgct tgctcctttt tcaagccatt
     1501 catccgcttg atcgatttta gattgtggta cattctcaat acgatccgtt gcagcatgta
     1561 aaagaaattc tctttggatc tggtcgtcag tccattgaac gataacctca gcggctccta
     1621 gtttataagc ttcttgtgtg atcaatcgag caaggggtgc ctgttccacg ctgatctgta
     1681 aaacaactgt gtgtcctttt tctgtggcta cgccagtttc tgcaattaag cgtgcatatt
     1741 tttttagaag ttcattaaaa tcagataaca taataaaaat tcctcctcac cattttttct
     1801 ggtaactatt agcatgatac catttaatta tgattcaaac aatgaggcct ttaaataaaa
     1861 aaaagaggtt atttttttaa ggttaagttt catgatatat gttactactt gctatttcag
     1921 cttgtgaaga aaatcaaaaa ctaattttgc atatcaaaaa aaacatgtta caatgaaatt
     1981 aatcaatggt ggaagcgtta taaaaaaatt aaggagatta aaaaaatatg gcaagaaaaa
     2041 agacgattac tagagaacag attttggcag ctgcttatga agtagctgcc accgatggat
     2101 ttacacgatt tactgcgaga aacatcgcga acaaaatgaa ttgttccacg caaccgattt
     2161 atcttgagtt taaaaatatg gatgacttga gacaagctct ttttgagcag atctatgatc
     2221 atttacaata tgaagtgttc actgttgagc atacagggaa tacgattatc gatcttgctt
     2281 ttaattatat ccattttgca agaagagaga agaagttgta tcgttcactg tacttagaag
     2341 aatatggtgg aggaaaagag atgcagaatt tctcttatac ttactttgtg aatacagtga
     2401 aacgcgatcc agcttatgtg aatctttcag aagagcagat cgaatctttg tataatggga
     2461 catggatcat agctactggg atcgcagctt tgatgtcttc aagcatcatc catccaactg
     2521 atgaacagat tgaaaagatg atacaagaca gtatagatgc tatcttgaaa ttcaaagaac
     2581 caatagaaat cgattgaata tactcaaaag ttccacaaat cagtagataa caagagatgt
     2641 gtcaaaataa ggaaaagttc gtttttctta cagcgtatgc atttttaact cccgaagaac
     2701 tcttccatga atgcaatgaa taaaagagcc gtacagattg aataaatctg gatggctctt
     2761 ttattgcaag tatattaaat ttttgcgact agttctttag cgaaagcttc taaattttcg
     2821 atatcttctt cttcagcatt cagatccact ttaacatttt cggctccttt tgatgcgcct
     2881 gtttttgcaa aagcagcatc gaaatcatcc accgatttgc agaattcatc gtaaaaagta
     2941 tcaccagatc cgcagacacc gtaaattttt cccgatagat ccaattcttg caagtcttca
     3001 taaaagtcta cgatctcatc aggcagatcg ccatcatcgt atgtataagt agctactacg
     3061 caaatatctg cctcctcgaa ttcatctgca tctacttggg tacattcgtt gatttcgact
     3121 tcgatatcca gattttcaaa tgcttctgca acaatatcgg cgatttcttc agtgtttcct
     3181 gtcatgctag catatacgat ttttgctaaa gccatgatgt gcctcctcgt ttataattat
     3241 ttaacaatta tgaattatac caaaacatca ttgaaaagaa aataaaacct aaagacacct
     3301 ggggaatgtc atttggctaa atgtatgccg ctatgttaaa atgagaggga tttgtaaaaa
     3361 ggagacgaaa atatgattac tttaaaatca gcacgtgaaa ttgaaatgat ggcagaatct
     3421 ggagctttat tagcagatgt acataaaaat ctgcgtgatt ttataaaacc aggaatcaca
     3481 agttgggata ttgaggtatt tgtcagaaac tatatcgaga gtcatggcgg aattgctgcg
     3541 caaattggct ttgaagggta tgaatatgcg acttgtatca gtataaatga tgaaatttgt
     3601 catggattcc ctcgtaaaaa gcctttgaaa aacggtgatt tggtaaaagt cgatatgtgt
     3661 atcgatttga aaggcggcat ttctgattct tgttgggcct atgtagttgg agaatctaca
     3721 cctgagatcg accgattgat ggaagtaacg aagaaagctt tgtacatcgg tattgagcaa
     3781 gcacaagtag ggaatcggat tggtgacatc ggtcatgcta tccaaactta tgtagaaggc
     3841 gaaaatctag cgatcgttcg tgattttatt ggtcatggcg ttggtcctac gattcatgaa
     3901 gaaccagcag ttcctcatta cggagaagct ggaaaagggt tgcggttgaa agaaggcatg
     3961 gttatcacga tcgaacctat ggttaatacc ggaacttgga aaatgaaaat ggatcctaac
     4021 ggctggactg cgtatacaag agacggcgga ctttcttgcc aatatgaaca tacattggct
     4081 attactaaag atggtccaag aattttgaca tcacaaggag aagaaggcac ttattgatct
     4141 atccagtcaa taggtacggg agaggtacat gaaagtttgg aacaaaataa aacaaaatga
     4201 aaatctgatg cgctttattg agacgactca aaagcgcatc actgattcag aaatgagtac
     4261 gacctctgtc gtcgttgctt attatttact cttgtcttta tttccattga ttattgcttt
     4321 tggtaatatt ttaccgtttc tccacatcga tcaagagact gtactgacct atttacgaga
     4381 agtgattcct gagaccgttt accagtttat cggtcctgca atcaaagacc ttttgacaca
     4441 aagttcagga ggcttgttat ccgtttctgc attagcggct ttatggtctg caagtcaaag
     4501 tatcaatgct ttgcaaattg cgatgaataa agcgtatggc gtagaaaata gaaaaaattt
     4561 tttgatcgtc cgtttcttct cactaattgt cattgtcttg ttcatgattg ctattagtgg
     4621 ggtaactatc gttcttgggt tgggacagct gattttggat gctattcagc caatcgtaca
     4681 aatacccgtg aactttatcg atcagttcca aactttgaag tggccaatca cattggtagc
     4741 tttatttgtc atcatgttct tgatttattt aattgtccct aatgctcagt tgaagttgaa
     4801 gacgatcatt ccgggcacga tcttcgctac tgtcggctgg atgctgttat cacaagtgtt
     4861 tagtatctat gctaggtatt ttgcaacgag gatcagcggt tatcagatca taggaagttt
     4921 tattgtactc atgctttggc ttaattttgc tgctacgatc atcattttgg gcggaatcat
     4981 caatgcagtt gttcaagaat acgtgacggg tcatcaaatc aaaaaacgtc gagatccaag
     5041 ccgaaaatgg ttcaataaat tgaaaactaa attttctcat aaaaaaaagt agaaacaaga
     5101 gttcttattg taaatttata tcagtaattt ataataatta tgactcttaa ttaaagtagg
     5161 tgcccatatg cttgtttctt ttagtatggt tataagattt tttctgacaa ttttattatc
     5221 actgatatta acacctgtat tcaaaatcat ttcagtacaa acaggtatgg tcgacaaacc
     5281 aaatgaaaga agaataaata aagttccaat gccttcagcg ggcggactgc ctatctttat
     5341 ttcatttgtt atatcgactt tattcttatt ccgtaaaatt atccctcaat cgtatattgt
     5401 accgatcttg attgcttctt tagtaatcat agtcacagga gtgctcgatg ataaatatga
     5461 gctgacacct aaacaaaaaa gtatagggat cttgattgct tctcttatta tttattttgt
     5521 agcagatata cgtattgatt ccttcacgct tccttttatt ggatatatcc aactaggctg
     5581 gcttagtttt cccgtaacga ttttttggat cttcggtatc acgaatgcgg tcaatctgat
     5641 tgacggtttg gatgggctgg cggcagggat ttcattgatc ggtttaatta cgataggaat
     5701 cattggatat tttttcctcc atgcttctac agtgtatatt ccagttgtta ttttctgtct
     5761 tgtagcaagt attatcggtt tttttcctta caatttttat ccggcaaaga tttatttagg
     5821 cgatacagga gctctattcc tagggtttat gatggccgtc ctttcactac aagggctgaa
     5881 gaacgtgact tttgtttcat cgatttcttt gctgatcgtc atgggagtgc cggtaacaga
     5941 cacgtttttt gctatcatta ggagaaaagc aaaccgtgtt tctttttcta cagcagataa
     6001 aaaacatctt catcaccgac taatcagttt aggctttact cataaaggtg ctgtcctgac
     6061 gatttatgcg atggctttga tgttttcttt cacagctatg gtcatgaatt atactggaag
     6121 aatcggaacg atcatattga ttattgccat gttgtttgct gcaattttat tatttgaatt
     6181 gattggtctg atcaatgaga aacatcagtt tattttaaaa acattgcgat ttttagggaa
     6241 taaagagtac cgtacgcaag taaaacagaa atatttgaag aaatggaacc gtcatataga
     6301 tgaataacaa attaaaccag ccgatcctta ttagtattgt gacggcaaac agtgataaaa
     6361 tttttcggac actagataat tttatttcaa ctgtaaaaga tgaaacagca gtaaaaatcc
     6421 gaatttttga caacaattca cttcccgagt acaaaaaaag actaacagag tataggaaat
     6481 atccctacat cgatatctat tttcatgatg aaaattccgg atttggttat ggacataatc
     6541 acaatctgat agctagcgat tctcgctatg gcattatttg taacccagat attttagtgg
     6601 atgaagagac agttacagca ttggtcttgt tattgaaaga acatccggaa tgtgcgatgt
     6661 tagcacctaa gattcttaat gaagatggaa caactcagca cctcatccgt gagcagctgg
     6721 ctgtttttga ttacgttttg cgctttgtgc ctttcaaatg ggtcaaaaaa aagtttgaca
     6781 aacgactggc tgtgtttgaa tgccgaggat tgcctactga tgctaatagt tatgtacgga
     6841 tgatctcagg tagttttatg gtagctgata ttgataaatt taaagagata aaggggtttg
     6901 atgagcgttt cttcatgtac tttgaagaca acgacttgtg tttgagtttt gaaaaagcag
     6961 gttacaagtt attgtacaca cccttttatg cagttatcca tttttacggg aagggtgcgc
     7021 atcgaaatgc aaaactgttt cgcgtgtttt tgacttctat gatgaaattt ttcaataagt
     7081 gggggtggcg ttttttctga tgaaaccaga aatattagta gtcgttgtgc tttatcagca
     7141 actattcagt caatctccag cttatggacc attaagcaaa gcacttagcg aaaagcaaat
     7201 tcagcttatt atttacgata atagtcctca aaggcagcaa aatgagctgt ttgaaaaaga
     7261 aaatatcgtt tatcatcatg atccgcaaaa tcctggttta gcggtggctt ataattttgc
     7321 gttgtctcaa gcagatgaag gaacacgttg tctcgtcact ctggatcagg atacgaggct
     7381 agtcgacgat tattttgaac tattgcgtaa agtaaccttt acagatgaat gtgttgctgc
     7441 agttccgatg attttttctg ggagccgaca agtttctccc ctatattcag atcgttatat
     7501 caatcgtaca gctcaagcag tagaagttga aggcccaaca gctgaacgaa tcatggcaat
     7561 caattcaggt gttgcctggt ctgtcaaatt tctaaaaaag attggcgggt ttaatcctgc
     7621 tttttcttta gattttttgg atcattggct gttttggaaa gtggatcagt tggaaaaaac
     7681 tgtggagatc cttcctgtac gaatggaaca tgatctttcg gttttagact atgaaaaagt
     7741 ttcaagtaat cgataccgtt ctattttaca agcagaaaat cgattttatc atgaatatga
     7801 tcaaaggcat ttatccagtc accgacgaca attattgtta agaacagcta aacagttctt
     7861 gaccgtcaaa aaccggcata tctggcggat gactttgcgc tcatatgtta agaattggaa
     7921 ggtgtgaatt cgtgcgttcg gtttgtatag ctacctttaa tggaggcaga tatttaagag
     7981 agcagttaga tagtatttta tctcagcttt cagcagaaga tgaagtagtc atttctgatg
     8041 atgggtcagt agatgataca tgggatatat taactgaata cgcagaaaaa gataagcgaa
     8101 tccagctttt ttcggggcct cgtcaaggat tgatcgctaa ttttggttat gcgatccagc
     8161 agacgaaagg agatctaatc tttttggcag accaagatga tgtttggttg gagacaaaag
     8221 tgaaaaagat cactgactat tttgagcaga atccggatca gctagtagta gtttctgatt
     8281 taatcattgt ggacagcgag ctgcaagaga tccatgattc gtatttttcg tttcgaaatt
     8341 ccaaagaagg atggcttaat aatgtgataa gaagtcacta tattggtgct ggtatggctt
     8401 tccggtcttc tttaaaaccc gatattttgc cgatcccgtc agaagtgccg atgcatgata
     8461 tgtggattgg tttgctggga ggaaagaaga ctgggttcct aagagaaccg ttaactctgt
     8521 accgaagaca tgatttaaac gcgagtgaga ttgaaacaca ttctaaatta cggcaaaaaa
     8581 tttcttggcg tgttcatctg attacggcgt tgatcaaacg aaaatggttt aaacgttgat
     8641 aaaagatgtt taaatcctta atatttccat gacaaatata acaattgatg agataataac
     8701 aaagtgaaat ttgaaatcaa aaaggagagc agatatccat gaagggaatc attcttgctg
     8761 ggggcagcgg aacacgtctg taccctttaa caaaggcaac atcgaaacaa ttgatgccga
     8821 tttatgacaa accaatgatt tattatccaa tgtctactct gatgttggcg ggaatcaaag
     8881 aaattctgat tatttcaacg ccacaagata caccacgttt caaggaatta tttggaaacg
     8941 gaaatgattt aggactgcag atcgaatatg cagtacaaga aagtccagat ggattggcac
     9001 aagcgtttat cattggagaa gaatttattg gagatgatag tgtctgcttg gtattagggg
     9061 acaatattta ctatggtggc ggtctgtcta aaatgttgca acgagcagct tcaaaagata
     9121 caggcgcgac agtatttggc tatcatgtca atgatccaga acgctttggt gtagtagaat
     9181 ttgatgagaa tatgcaagct ttgtcgattg aagaaaagcc tgctcagcca aaatcaaatt
     9241 acgctgtaac tggcctttat ttttatgaca atgaggtaat ttctatcgct aaaggtatca
     9301 aaccttcaga acgtggagaa ctagagatta cggatgtcaa taaagcatat ttggataaag
     9361 gaaaactatc cgtagaactt atgggacgcg gatttgcttg gcttgataca ggaacacatg
     9421 aatcattgct tgaagcatct acctttatcg aaacaattga aaaacgtcag aacttaaaag
     9481 tggcttgttt ggaagaaatt gcttaccgta tgggctacat cgataaggaa caattgctgg
     9541 cccttgcaca gccattgaaa aaaaatcaat acggtcaata tttattgaat ttggccgctg
     9601 aataaggagt ccttacagat gaaagtaaca gatacaaaat taacagatgt caaaatcgta
     9661 gaaatggacg tctttggcga tcatcgcggt ttcttcacag aaagttattc aaaagcaaaa
     9721 tttgcagaac atggattgaa atatgacttt gttcaagaca accattcact atcagctgaa
     9781 gctggtgtcc ttcgggggct tcatttccaa aaaggagaag ctgcacagac aaaattgatt
     9841 cgggtagtca ctggagcagt tttagatgta gtagtggata tccgcaaagg tagcccgact
     9901 tatggtcaat gggacggatt tatcttatct gaacataatc atcgacagtt gcttgtacct
     9961 aaaggatatg cccatggatt cgttactttg acaccaaatg tgaacttcct ttataagtgt
    10021 gataattact ataatgcaga agcagatggg ggcatcgcat ttgatgatcc agatttagcg
    10081 attgattggc caattgatcc atcaagagcg attatgtcgg aaaaagatca ccgtcatccg
    10141 acattaaaag aatttgaagc agaaaatcca tttgtttacg gagaaatcta agaattaagg
    10201 agaaattttc ttatgaaaaa catcattgtt accggaggtg ccggattcat cggctctaat
    10261 ttcgttcatt acgtggtaaa caatcatcca gaagtgcatg tcactgtttt ggataaactg
    10321 acttatgcag gaaacaaaga gaacttggct ggacttccta gcgatcgcgt agaattggtc
    10381 gtaggagatg ttgcagatgc agaattagtg gaccgtctag taaaagaagc ggacgcagtt
    10441 gttcattacg cggcagaatc ccataacgat aattcattaa aagatccttt cccatttgta
    10501 caaacgaata tcattggtac gtatacactt ttagaagctt gccgcaagta tgatgtacgc
    10561 tatcatcacg tatctactga cgaggtttac ggcgatcttc cattacgtga agacctgcca
    10621 ggacatgggg aaggcgaagg agaaaaattc actgcggaaa caccatataa tccttcaagt
    10681 ccatattctt ctacaaaagc tggttcagat ctgttagtaa aagcatgggt gcgttctttt
    10741 ggcttacgtg caaccatttc aaactgttca aacaactatg gtccttatca gcatattgaa
    10801 aaatttattc ctcgccaagt aacgaatatc ttaagcggta ttcgtccaaa attatatggc
    10861 gaaggaaaga atgttcgtga ctggatccat acgaatgatc attcttcagc agtgtggctg
    10921 attttgacga aaggcagaat tggcgaaacc tatctgatcg gtgcagacgg tgaagaagat
    10981 aataagaccg tgatggaatt gatcttagaa atgatgggtc aaccaaaaga tgcgtacgac
    11041 catgtaaacg accgtgcagg acacgacttg cgttatgcaa tcgattcgac aaaattacgg
    11101 gaagagttag gatggaagcc tgagtttacg aacttccgtg atgggttggc tgacacgatc
    11161 aaatggtacg aagaacatga agactggtgg aaaaaagaaa aagaggccgt tgaagcagca
    11221 tatgccaaaa atggacaata atacagagaa aatgtcactg tattaatgga aaaacacatg
    11281 tgaggcaggc gctcgcctgt ctctttttta aaataaaaat caaggagcgg ataatcatat
    11341 gtttttatta actggaggaa atgggcaact aggtacagaa ttgcgccatt tattagatga
    11401 aaaaaatctg caatatgttt caacagatgc aaaagacttg gatatcacag atgccgagaa
    11461 aacaatggct tacatcacag aattaaaacc tgatgttatt ttccattgtg cagcttacac
    11521 agcagtagat aaagcagaag aagaagcaaa agaattagat gagaagatca atgttgatgg
    11581 tacaagaaat gtagcacttg cagcaaaagc tgtgaatgct caattcgtct atatcagtac
    11641 agactatgtt ttcgatggaa aaaacaaaaa tgtagaatac gaagtagacg accaaacgaa
    11701 tccactaaat gaatatggtc gtacgaaatt attaggcgaa aaagcagtag aagagatttt
    11761 agaagactat tatatcatac gaacttcttg ggttttcggg atctatggac acaatttcgt
    11821 cttcacgatg caacgcctag cggaaacccg cgacaaactg actgtagtaa atgaccagtt
    11881 tgggcgacca acttggacac gtactttggc agaatttatg gtatatgtca tcgaagaaaa
    11941 agcaccattt ggtatttatc atttgtcgaa cgaaaatagc tgttcttggt atgaattcgc
    12001 aaaagaaata ttgaaagata cagatgtaga agttgcacct gttacgtcag aagaatttcc
    12061 gcaaaaagca acacgtccgc agtattcagt catgagcctg aaaaagacag aagagttagg
    12121 atttgtcata ccaacttggc aagaagcact tgcgcaaatg ctggaagctg ccaaaaaaat
    12181 gaataagtaa tttcctttta tcaaatagaa tttggagcat gatatggaaa aacaaacaag
    12241 aagtgcacga cgaaaagaaa agccaaaagc gaaccaggca gattcgaatt tggtgcttga
    12301 cctgtttcta aaactccgct atcttattgg cgttttagtc atcgtggtca tcgttacttt
    12361 taatttaaac ggaagttcac ttggtgtttg ggataaatat gtgtcgcaaa gagacgatgg
    12421 gaaaaaaact gatgtgattt ttggacagaa tcgggaagtg cggtcagacg aatggctggt
    12481 gcagacacct ttttacctct cacaagctga agaggacttt ccgttggtga acgaggatta
    12541 ttcattaaac ggacaaaaca tgatcattgc gtataattct cctgcaaagg atattacggt
    12601 tatcggaaaa ccattcaact ggggattctt tttcctcgga agagatcgag gactttcctt
    12661 ttattgggcg atgaaattag tcgtcatggt cttgcttgga ttcgaagtac tgatgatatt
    12721 aacgaagaga aaaaaagctc tatcgctaat cggtgcattt gcgctgacat tttctccagc
    12781 agtccagtgg tggttcatgc aacatgtggg cgatttgatt ttctttactt tgggactgat
    12841 gactgctttt tatcattatt tctacaatca tgataaaaaa tggctacgaa cgttgatgat
    12901 gcttttggtg gttattttcg gtattgggtt tattttagtg atttacccag cccatcaagt
    12961 gatgctggcg tacttgctga ttttttattt tgttggcctg cttatttact atagcaagaa
    13021 agtttcatgg gatctgttgg atgtagtgtt gattggaggg gcagttttat ttattggcgg
    13081 agtcatggtt catttctggc taacgtcaaa agacgcattg ttagcttctt tgaatacctt
    13141 atatccagga aaacgggtaa gtactggagg aaagtggacg attggcgaat ttttttcttt
    13201 ccttacaaat tggaaaattc cttttgaaga tatcaactat tcgaataact ctgaagtggc
    13261 cctgttctat cacttttttc caactgtatt cctagcaagt ccatttgtgt tatttggcaa
    13321 aaaagatagc gaacaaaagt tactgggacg tattttgatg attttttgtc tgttttctat
    13381 cttttggatt accgttgggt tgccaaaatg gctagcagaa atcactctgc tgtcttatgt
    13441 tcctccatta agagccatcc ttacttttag ttttgcggct tgtttattat cgatctggtt
    13501 tatcgcttat atttggagag aagatgttat tcctatttgg tataaattca ttttgttggc
    13561 agtaaatgca tggtcttatt tctatgcgat tacgcattcg aaaatagata attatttttc
    13621 ggattttgaa accatcgcag ttgctgtgtt gggaacagtg attttagcat ttgcattatt
    13681 tggctggaga cgcttgtttt acctcgcttt tgccgcctta gttatcgtat ctggcttttt
    13741 cgtcaatcca gtcgtagaag gtacaggagc gatttttgaa aaaacactgg caaaagaaat
    13801 ccaaaaaatc gatcaacaag atcctggtaa agtttggttg tctgaagatg aattatataa
    13861 cttcacccca acattgggag tgaaatcctt caatacagta cgcttttatc cagatatagc
    13921 tgcatggaag gaaatcgatc cgaaagaaga atacgagaag tattataatc ggtatgctca
    13981 tacaagagct tttatcgcta aggaagatac aaagttcatt ttagatcgtc cagatatgtt
    14041 ttcggtcacg ttgaattttg aagacgcaga aaaattaggg atcgactatg tgatcagtaa
    14101 acgaccatta actgattaca accaattata tgaagcgcag ttcacgcagc tatatggacc
    14161 ggataaagac ggttatatga tttttaaagt agaatatccg gattatccag atgtaccaca
    14221 aacacaaact caggaaaatc agctagtcgt acagtaatag gctagctgag aataatcctg
    14281 aaatattagt agaaaggaga gtggagataa ttcaagcaat cgtacaaaaa tttgtcaaca
    14341 aaatgagtgt cggtttcttg cttttgttgc tgacgctagt cttttttgtt tggacagcag
    14401 ggttaggttt tccagcaact ttgtttatat ataacaatag ttcattactt atcgctttta
    14461 gtgtggctat tttgctcatt ttaaatgttt ataaagcaaa aggcgtcgat tttctttatc
    14521 taggtgttgc gcttgccctt ttcattttct tttcttactt tcgggaatta agaaatgagt
    14581 ctttatttgg agatttactg tttcctttga ttattttatt ttgtttagta attaaatgga
    14641 tcgagtggac aaaatgggat cgcggtattt atctgattgt ctttacggtc ttcctcggaa
    14701 tcacgttata tcgtgtattt acagagatcc aagtaccaga aggacaaagc atctgggctc
    14761 caggcaattc gctttctgat atttggatca atacgaatac aatcggaagt tctttgatga
    14821 ctcttgcttt attgatcagt ggatttgcca gttcttttga acgatggtat gtacgaatca
    14881 tcggtgcagt agcagttgtt gcagcttttc tcgggatttg ggtctgccaa tctagaggtg
    14941 cattagtcgc gttgattgtt tttgcgatat tggatctttt acctaaatcg ttgatgcgtg
    15001 tcattcgtgc gccatttatt gcttacacgg ttacgattct tttagctttg ccgatttctt
    15061 atctggcggc tgtctctgaa aaagtcaatt tgtttaccgg tcgagaagat atttggcata
    15121 aattctatca gacgctagga gaaaaatctg aacaagtttt actaggaatg aaaacattta
    15181 tcttccaacg aggaaaccaa tttttaggaa accacaatag ttataattct attttgaata
    15241 tctatggatt gataggattt gggattgcta ctttgctgct tatccttttt attggtcgct
    15301 taacgttgaa agcagattta tccaacgggc agatgacttt tatctgggcc ttttttgcgg
    15361 tgatgatgca gtcatttatg gaggatacat tgacttcata cccatgggtt ccgattattt
    15421 atatcctgtt ggctatggcc gttcatcgat atgatgaacc agaagttgcg gaggaattct
    15481 atgttatgga agaggcatat gacgatatga cttatgatga tacatcttat gaggaacaac
    15541 cagatagcgc tcattcttcc aggatccaac gctatcattg ataggcacaa aat
//
LOCUS       DAZUMV010000056        15572 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000056 DAZUMV010000000
VERSION     DAZUMV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.056"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..1470)
                     /locus_tag="ACG8FK_001572"
     CDS             complement(106..1470)
                     /locus_tag="ACG8FK_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5097182.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1467..3026)
                     /locus_tag="ACG8FK_001573"
     CDS             complement(1467..3026)
                     /locus_tag="ACG8FK_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5097183.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3082..5562)
                     /locus_tag="ACG8FK_001574"
     CDS             complement(3082..5562)
                     /locus_tag="ACG8FK_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097184.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5546..5881)
                     /locus_tag="ACG8FK_001575"
     CDS             complement(5546..5881)
                     /locus_tag="ACG8FK_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097185.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5895..6929)
                     /locus_tag="ACG8FK_001576"
     CDS             complement(5895..6929)
                     /locus_tag="ACG8FK_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5097186.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(7019..7699)
                     /locus_tag="ACG8FK_001577"
     CDS             complement(7019..7699)
                     /locus_tag="ACG8FK_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5097187.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7983..9086)
                     /locus_tag="ACG8FK_001578"
     CDS             complement(7983..9086)
                     /locus_tag="ACG8FK_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS5097188.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9715..10185)
                     /locus_tag="ACG8FK_001579"
     CDS             complement(9715..10185)
                     /locus_tag="ACG8FK_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS5097189.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            10515..12155
                     /locus_tag="ACG8FK_001580"
     CDS             10515..12155
                     /locus_tag="ACG8FK_001580"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS5097190.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12309..13631
                     /locus_tag="ACG8FK_001581"
     CDS             12309..13631
                     /locus_tag="ACG8FK_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS5097191.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13699..14133)
                     /locus_tag="ACG8FK_001582"
     CDS             complement(13699..14133)
                     /locus_tag="ACG8FK_001582"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5097192.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(14198..15034)
                     /locus_tag="ACG8FK_001583"
     CDS             complement(14198..15034)
                     /locus_tag="ACG8FK_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS5097193.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         4552 a         3137 c         2813 g         5070 t
ORIGIN      
        1 atattatgct caaaaaattc ttccgctatt tcattatatg aattttttat tctcgattga
       61 ttaatattta ttatatcccc acacaataaa agaccgtgat atttctcaca gtcttttatc
      121 tactctattg ctatttgctc tcttagctag tactttaaaa gctaactcat tgaggtagtg
      181 aaaacgagag gtgtgttcag acagaaatcc aaaatcctca actgctagct gtggaatcgt
      241 agttttgtgt ttcacgatat ccgcaagcag gttcaaatct gataataaga attcttggct
      301 tatcagctgt ccgccaatta tttctaatgt atccggatgg tagatcagct ttgcatttac
      361 tgttttgttt tcttcatcgt attgtggaaa tagatcaaat tctcctgaac acgaaccggt
      421 attttgttcc aagaatggcg cttcatcttc tgtcagccct acagttccta aaaagtagtc
      481 tataatgact gctgctgtta tcttttgtct gaggtcgtaa gcggttggct gaccactcaa
      541 agttagcgct actgctcttg ccatatgtac agctcgagta actaaaggta aaaagagatg
      601 ccgtttattt ttgtcaaaac tgactgggac gatatctcca atcgcataga tgtccggatc
      661 acttgtctgc agatattcat tgactatcac agtctcatcc tcatacaaag ccactttttc
      721 ttttaacaat gtcgtattag gacgagtttg ggcagaaaaa atgaccatat ctgcctttat
      781 tgcttgagat agaagttgta cggatactac tttctcttct gattttttcc aatcgatcaa
      841 gaattcattt ggataaaaat tgatttgctt agtttgcagc tgattttgaa aactgttgat
      901 catatcctta tcgaaatatc ttgttaaaac attcggcata taatcaacta gatgaataat
      961 gtatccttta tttctcaaag catctgctaa ttccaatcca atataaccag caccgaagat
     1021 cactacttct tttgcttctt ttaaagcggc aagtacttgc ttagattgca ctagattttt
     1081 ataggtgtag acattttcag cttcaatttg tttatacaag gtgacaggag aggaacctgt
     1141 tgctacgatt aatttatcat aggttatggt ctcgtttttt ccttctttgg atgcgaggat
     1201 gagctgatgt ttgtctggat caagttcagt tactctccat tcggtgatca gttttgcccc
     1261 actttcttga agagtccgca tgttatgggc aacttcagct aaactacgta tctttccttt
     1321 taaataaagg ttgattccat ttgaaacata gcctagtcca tctttatgat cttcatcgat
     1381 cagtaaaact tctgctttcg gttgaagttt tcttagattt agggcagctg tgatgcctgc
     1441 atgggaagca ccaatgatca cgattttcat gttttattca tccttaaatt caatttttct
     1501 aaataacatt cttttaaaaa ttggttcaaa taggggaaat cataattata ttcgttgcta
     1561 gtgaatacaa aaactttagc ggacttctgt gacgggtact ctttcaattg gctagtcaaa
     1621 atgatatcat actgcttttc tttctcgtat tcgactaaat catatttgat cgtattcagc
     1681 aattgttcct ggatgatttc tttcacgata tccgccatta gcctttcata aggaagatca
     1741 taagcaattt gcagacgtaa ttctaactgc ttgatcagcg tccgcaaaat agatgcatag
     1801 cgagcaaata attcaaagcg ggcattttcc tcgtctactt cagtaaaatc aacaatagca
     1861 aatgtttgtc tgaccaatct ttcacataaa accaggaatt gtttatcttc tacttcagtt
     1921 aaccgctgtt ttaatccttc ttggccaaaa atattcatcc atcctctaaa ataggcaaaa
     1981 tgagcgtgat tttgcatcac tgtcaacgca atcttttgcg aaatctcttc tggaaaaaca
     2041 taaaaagaga aatattcttc tatcgattgt ttaaacgttc gattcaattg agctactacg
     2101 tcgtctttgt atttttcgct tatcaaaaac tcctggacat gcgagttatg tgtatctaat
     2161 gtaaagttac tgatcatgaa aagataaaaa accaacgttt cattttgatt ccaaggaaag
     2221 gcatattgaa agaagtaacg ggtcaaaaat gtatggaggt ccaataaaaa ttgtgtcgtt
     2281 tgaaacaatc caacaggaag attgctataa tctggtttgg attttttcgt acagcgcatt
     2341 cgcttcttca taatggaaag ccatatttca aatttcaatc gtccttcttc tgtaatagta
     2401 aaactaaact ctttttgcag cagatctaaa aaagtgtttc ctgataaatc actgtattct
     2461 tttttgtttt caagataagg acgtccaaac caaaataatt ggaaataaaa ataacaaatt
     2521 tgactttctg gaccggtcag ccgcccgctt tttattttca gatgaaactc atttaaagaa
     2581 tcatttaatt ccttcactct gcgagacaaa gtagccgaac tgatattaag ttccatgaca
     2641 agtttctgcc gatcaaatga gccattttcc aacagataaa gaagtatctg atattttgta
     2701 gaatagcgca agtagcaatg ataataaaca tcgactgctg ctatatttcc atgtctgatt
     2761 aataaaatat tcccttcttg gataacaatt tccaattgtt tgatattttc caagttagct
     2821 tgtaaataaa acagatcttc tttcaaggta gcccgatcaa ctgataagcg ggtaagtacg
     2881 ttattaacgg aaatctctcc tttttcctct aataaaagtt ggaaaagttt gatttgccgt
     2941 tgttcatttt tttcaagcaa actgaaaagt tccaagtcca ctcctccaag catttctgat
     3001 tttaacagca gaagtttcct tctcatcctt gaaaaagagg atctgtgaca gttatcacag
     3061 atccatacaa attctaatca cttagctggt tggcccagct gtcagttccc atgtgaccat
     3121 gccttgatac gttgcgccag gttgagcatt cgtactgcta tttacctcta agagtaaacc
     3181 atcggtatcg ggaaagtcaa cgagaacctc tcctttagct gttccatcac tctcataaat
     3241 caacgtgttt tgaccttgct gaatcaattg ttttgatcca gaatgattga agtataattt
     3301 tgttccgctt aattcttgac cttgatcatt cttaaataaa gaagtcaatg tccctgtcag
     3361 ttgccaagga gacgtgcttg gaagccgagt atcccgcaca tcaaaagaaa aatcgccttt
     3421 tgcttcataa taacttgtct tcaacggttt ggggagatcg ttaccaaatg taaaatcatc
     3481 tggtacatta actaatgtta attcttgttt ggggacaaga attgttttag cagatgtttg
     3541 gacaactggg ttaggaagct tagcaccatc tgctaatgta atattgctgg aaagtacagc
     3601 tgcctttgtc agtatgctga cggattgtac agaagtatct acagtattta attgatagtt
     3661 gtaagtaaga tgaatggtat ctcctatttt gctcaataga tctgctggga cagaaactcc
     3721 ttgttgaatg ccggtatctg cgatagttgt accatttaat tttaaggaac cattgatata
     3781 agtagtattt tcaggaagtg agatcgtgat ttttttgtcg gttatcgcat ccccagattt
     3841 tgttaacgtg aatgtcacat tttcttgaat cgtatctcct gatagagcag acaggggatc
     3901 tcctgtctca ggttgtctgc taaggttttt tagctgacta tctgcactca cagatggaaa
     3961 attagccact gttatttgag gaagagtggt gattccagct tgttcagact gccatttgta
     4021 cgcaaaagac ggatacatgt tagtaagtac atcgtccacc aagcgataat caaaataata
     4081 ttgccctgaa gccaatgcag gtagtgtaga agtagctgta tttacacccc cgttatttga
     4141 ttggatcgtt tggaaaagtt gatctgtcca tcctgtcgct aatttttggg ttcctttcga
     4201 ataaagcctg taatggatcg aacccttttt tagttcagtt gctgaccaat tggccgtgac
     4261 ttggtacgat gcagaagaaa ggagctttcc ccactgacta tcggctgtta agtttgttgg
     4321 taaaaccgtt ccgtctagct tgtactgctc caataccttt aaagcaacag gagaaagcaa
     4381 tccgccttgc gcatctttcc cactgtacac gatattggct gtctgattca atccacttgt
     4441 gcttgctgga gtatgagtca gtattgctcc ttgtgtaacg ttttttcctg taaaatagtt
     4501 gactacgcga tcaaagcgac taaatggcaa ctgatcaaaa cgtccttcag tcagccccat
     4561 cgtcaatgtc cgactattgg tagagcctgg aaggttctgg ttatttccat ccacgtattc
     4621 tgtggtatca gcaaatgtat tatattttcc tgttgaatca cttgttgaga tgtaactatt
     4681 ccacgtattt atctctgcac ttgcagaaaa tgcatatgct cctggattca agtttccgcc
     4741 agttaattta ggataaaatc tacctgcagc tggagctaaa atcgaattga acatacgatc
     4801 ttggataata ttattcgaat cataaaaatc aacagaaaaa acggcctttt tagacataga
     4861 aggttgtaat ttacccttgg tcaaaatttt gtttggactc gtatcatatt tatctccgtt
     4921 ttctgtcatc ttcgcgacat tagctgtaac atcaaattga agcgggatat cgtccacgtt
     4981 gaattcgcct cgcgtaataa tagcaataat atctgccaat agagtatttg tatcccacga
     5041 ccccatccca aaacgcaagg tatggcttcc tgctgataat aaactaccgc tagtaacctt
     5101 gatgggggca ccggcaatat acaagtaggc gttgttccag tcaattgctt ttaatacatc
     5161 actgcttgat acatgttccg gcaaattgat gatcgccaca aatgcaaatc cttgtgcagt
     5221 gatattcacc aaatcaagta catttgttag tttcacatgg ctttcccaat gaaaacggac
     5281 cgtgccacct ttccaaggat catttgaacc atcgttcaaa taagtagcag tcaatttagg
     5341 caattggtta gccccagtta gttggacaag cttcccgcca ttgactgcac tacgtccacc
     5401 atttggaggt acgatatttt catacgtgta tggatcataa gaaatggcag gaggcgttgc
     5461 tccaacagct tctgccgaag gtgaaaagat aaatatactt aaaacaaaaa gcgaagtaaa
     5521 aaagaaggag cgaaaaaatg aaaatttatt ttgtagcaac atgttcatcc tccttttcct
     5581 ttcttctcca taacaacaac cacgcaagaa gccataattc tataccaatg atcaaataaa
     5641 gaaaacggat cttttcttca ttcgtctccg ggaatacgcc atgttgatta gattctttat
     5701 aaatcggtag ctgtgtctgc tgttttgatg cgccattgac tgaaccactt ggatgcggaa
     5761 ctggacttag atcataaaat gtaatcgtac ctgaactttt ggtatctgct tggactggct
     5821 cttgccagct gaagaacagc aaacttgcaa taatccctcc caataagatc gatgttttca
     5881 acgaaatcac ctcatcatgt tgtttctcct tttttctttt tcttgtggac ccacaaaaac
     5941 agcaaaatca accataacac tagaactgca gcaaccaatc ctgcataaag ccatgggaaa
     6001 gattgttctt gtactgcaaa aacggaattt tcatttactt tttgtgcttt ttcttttttg
     6061 atcgtaaagt tttgtgtaaa tcgccattca ttactgtcgc ctgattttgc tatgatttct
     6121 gcgcggtaat cgccggcttt tgctttatca ccagctaaat caagattcaa gtgaaaattc
     6181 gaatttggtg caactaaaaa ttcattagac gtattttcaa tataaagctg attttcataa
     6241 aagatcttgc tcgtaaatat caaattgctg ataatcctag gctgaggatt ttggaaacga
     6301 agctgaacag ttggctttgc gttgacctcg tccaaatctc ctgataaaag tttcagatcc
     6361 ggttctatcg tctcttgact ttcttgtaaa acgactgcga gattataagc aatacgattt
     6421 ttgatttgtg ctccttctgt agcattttct gtttcagctt cttctttagg tgaaacatga
     6481 atacccgcta atacacgtcc cttaaagctt tttgctggga ttttcacttc tataggtata
     6541 gttgtttctg catgtgctga tacattgatt tcctttttcg caatgagcaa aacgtcacgg
     6601 acatcaaacg gcaatgtctc atctaacgtt gggttttttt gactataatc gatcgttcca
     6661 ccatcactcg ttaccgccgg attgacagag acctcaaatg ttcgttcttc atctgaagaa
     6721 ttgctgataa ttacttctag tgtctgattt tgttccggtg ccactaataa atcaaaataa
     6781 ccgattgctt tatctatttg gttctctgga aataccggtt tgattccaaa gtctcctgct
     6841 gaatcctttg cttggctgat tgacggaaaa atgaaaaata gacttaaaat agaaaaaaac
     6901 gaaacaaaca gtcgtttact ttgattcatt gtccaccaca tcctttcaac aacaactgcg
     6961 gtaaaaaatc cttcacctat agtattttca ccgcagtttg ttgaatttat ttcatactct
     7021 atgctactgg gccagccacg aggttccaag tcaaatcagc cgtgaatgtt ccagctgctg
     7081 ctttcggcac tttcagtgtg atatttgctg gatcataaac agaaagccat gttcccagac
     7141 cttgatcttt tgctgctgta aatattggtg tcgctgcact tgctttactc aacccagtta
     7201 cagtttgact agttggtcca gtggattcgt tatctgacgt acttttaaca gtggatgttc
     7261 caaaatctaa ttcagcgcct tgcaaaactt gtgaaccgtt tacaaaatct gaaaccgaaa
     7321 ctgtcacgtt ccacccttgt gcttggccat ctgcacctac acctcgacga tctgatactt
     7381 ggagataagg tgtatcgttt tttgaagcgt ctacttgata cgtttttgtt ccgctttcaa
     7441 tctcgtgtgt accaaatggc aattcaggaa ctacatccaa tgttaaagaa cctgtctggc
     7501 cagttgctgg attttccgga tctgctggct ctactggttt tgaaggatca gtagggttaa
     7561 gaggctcaac ggtatttgtt ggttcagtaa aggtaatatc tccttttgaa gtcgcttgac
     7621 cctcagcaaa agctggtgtt gccaagccgc ctagaagaag tgttgaaagt aataatgtcc
     7681 caatcatgtt ctttttcata ataaatcact cctttttctt agtgacctaa gtatagtaga
     7741 gacagtatgc aaaaaatcct catgaattca tccgcttact tgaaattatg accctttttg
     7801 tataaaagag aaaaaaacaa taaaatcgtt tacatgaata ttcttccttt tttattaagt
     7861 atgaaaaaat atttcaattt tattttttcg tttcaaatta ttctattagt gacatccttg
     7921 aactataaaa ataggagtac ggcatcatct gccgcactcc tatttttttc gtcatttatt
     7981 tcttaaataa aacgattctc acgcaatttt atttttttga tatcttctac tgtttgtgtt
     8041 ccagctaatt gcatgaccat ttccaattct gttttaaaga aatcaaacac ttgatggact
     8101 cctgtacttc ctcctaaaga caatccataa ataacgggtc tgccaatagc aacaagatcg
     8161 gcacctgatg cgattgcttt gaaaacatgt tgtccacgtc gtacaccgct gtcaaacacg
     8221 attggtacac gtttatctac tgcttctgcc acatattgca acgaatcaaa cgcagcaggc
     8281 cccccatcta attgacggcc accatggtta gatacccaaa ttccaccagc accagattct
     8341 aatgatcgat agacatcttc ttctgattga acacctttca cgtagactgg caaatcagaa
     8401 tgagcagcga taaattctac atcttttgga ctaagttttt gtttggaaga tttatataca
     8461 gcatccatcg tttggcctac tcctgattga taagcctgaa cgatcggcat tggcagcggg
     8521 aaggtaaacc cgtttctgcg gtctgtttca cggtttccgc caactgttgc atccgctgtc
     8581 aatacgattg cttttgctcc attacgcttg gccacttcaa gaatatccaa attgattcca
     8641 ttatctttgc tcatataaaa ttgaaaccat tgcggtgctt tctccccgcc tgcttctctt
     8701 atctcttcta aagtacaaga agcgtaagaa cttgctgtat agatcgtccc aaaatctgct
     8761 acaccttttg cagaagcttt ctccgccttc acatgagcta atccatgagc agcaactggt
     8821 gccatgatga ttggtgcagt cagcatttct tcgtcaaaat gtgtagttgt atcgggtaat
     8881 tctacatctc ttaatacatg aggaataatc aaacgatgat tgaatgcccg ttcattttct
     8941 tgataagtaa ataagtcgcc agcaccactg ctgatgtaac cataaccacc agctggaatc
     9001 acttgtgaag ccgcgatttc taaatcttcc atattgataa aatctactat tccttcattt
     9061 gtacctgctt gatatgtttt ttccatctca atgccttctc tctataaaat ttgaatgatg
     9121 aacaatatga tagcgtttac actttttata ttactcttcg acattgatac aggcaaataa
     9181 tctgtttaca tttcagccat tccagatgtc actactctgg aagaaatgat tgtaataaaa
     9241 atagataaaa aaccgatggg actttgacaa aagtagtgtc aaagtccctt tttttgaata
     9301 aacggtgttc aaaagccatt ttctcggtat taagtcaaat ttatgcaaaa atgtggaaag
     9361 ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac aacctctcga
     9421 taaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaatggca aaatatgagg
     9481 aactattttg ccatttgtct ctctaatact caggtcagaa cgttgacttc acagtctctt
     9541 ccttaaacgg tgttcaaaaa cttgcatctg cggtaataag cccaaccaaa cgaaaaatag
     9601 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agggcaggtt tttcacgacc
     9661 tctgattcac ggtgtttctt tttcatcaac atttttacga aatactgtgg aagactattg
     9721 aaataagtgt ggattgtttc tttgtgtttc aatcgtttct tgccattctt catttaattt
     9781 tggattaccg ttttcttcct tgaactcata aggtcctaat tcaaaaatcc cagacagtat
     9841 ttgattcacg atttcatata gttcagtgtg atcagacaca tgattcttga agatcatctc
     9901 attatcacca cgtttgaacc tcacattcgg gctctcatga tgaacctcta tgcatttttc
     9961 tccttttttt aactgacgga cttctgaaaa catctcataa atatcttctc cagtaaaacc
    10021 ttcagcaagc gccagactgt ccacaagacc gcctactgtc agttcagaga ttattgcttg
    10081 attggaatct aattttaatg tatagcttgt cgcttctact acgccacatg cattgcaacc
    10141 gccttctgat agttcgcttt ttctaatcaa ttgaacttct ttcattttct cttcctcctc
    10201 ttaattttct acttatgtat ttttatcacc tgacataggt gactcctatc aacgctatca
    10261 tgtgcttttt tcctcagaaa tcctatttat ccattccatc tatcccttat tcttctactt
    10321 ttctttctct agcatataag agatacgcat agtattcaag caaggattgt cgatataaaa
    10381 agaacaaata ctgctatttt gatttctttt ggctatatta catataaata aaatttatca
    10441 tacctgctct ttttgactat atcatcatct gacttctata cttcgtttgt attaaaactt
    10501 aggaggaatc attcatgctt acagggttaa acttattaaa taatcctttt ctaaataaag
    10561 gaacagcttt tacaaaagaa gaacgggcaa aatatggaat cactggtatg cttccaagta
    10621 ctgtccaaac actggaacaa caatctgttc aagcttatgg tcaatactta agcaaacaaa
    10681 cagatttgga aaaacgtatc ttcttaatga atctattcaa tacgaatcgg acattgtttt
    10741 ataaattgat gggcgaacat ttagttgaat tcatgcctgt cgtttatgat cctgtcgttg
    10801 cggattcaat cgaacaatac aacgaaattt tcttgaaacc gcaagatgct gcatttcttt
    10861 caatcgacga accagaaaac atcaaagcaa gtttgaagaa cgcggcagat ggacgagaca
    10921 ttcgtttgat tgttgtgact gatgcagaag gaatcttagg tatgggcgac tggggtgtga
    10981 acggtgttga catcgctatc ggcaaattga tggtctatac tgctgctgct ggtattaatc
    11041 cagcacaagt tttgcctgta tcgatcgacg ctggtactaa taatgaagaa ttattgaata
    11101 atcccttata cttaggcaat cgtcatgctc gtgtagaagg cgaaacttac tatgaattca
    11161 tcgatcaatt cgttcaatca gctaccgaat tattcccaga attgttattg cattgggaag
    11221 attttggacg aggaaatgct gcagcgatct tagaaaaata cgaagatcaa atcactactt
    11281 tcaacgacga tatccaaggt acaggaattg ttgtcctagc aggtgtatta ggcggattga
    11341 atatatccgg tgaacaattg aaagatcaaa taattttgac atttggtgct ggtactgctg
    11401 gtgtgggtat cgcaaacatc cttttagatg agatgatccg tcaaggtgta cctgaagaag
    11461 aagcacgcca acatttctat caagtagata aacaaggttt attgtttgaa gatacagaag
    11521 gattgactcc tggtcaaatc ccattcgctc gcaaacgctc agatttcact aatgctgatg
    11581 aattgacaaa cttagaagca gttgtaaaag cgatccatcc gacaatcatg attggtactt
    11641 ctacccaacc tggagcattc actgaagaaa tcgtgaaaga aatggctgct catacaccac
    11701 gtccgatcat catgcctttg tctaatccaa caaaattggc agaagcaaaa gcaaaagatt
    11761 taatagaatg gacggacgga aaagcattag ttggaacagg tatccctgct gctgatgttg
    11821 aatataatgg tgtaacttac caaatcggtc aagcaaataa tgctttgatg tacccaggtt
    11881 taggacttgg tttgattgct tcaacagcta cacgagtaaa tgctgaaatc atttctcaag
    11941 ctagtcgagc attaggcgga atcgttgatg taacaaaacc aggagcagct atcttgcctc
    12001 cagtagcaaa aatcactgaa ttttctcaaa cgattgctga aactgtcgca aaatcagttg
    12061 tcgcacaaaa cttgaatcgt gaagaaatca cggatatcaa agaagcagtt gaatctgcta
    12121 aatgggttcc tgaatataaa tctttagaag attaagatcg ttatagtaaa agaacataca
    12181 gaaactttgt atgacgtcta aaaagaaaaa agcgactgcg gcaaaacagc ggccagccaa
    12241 ctcctgctct tttgtcgctt ttctttggct tttttcatat gatacagaat aagaaaggat
    12301 ctgtaaaaat ggcacaaaaa acaacaactg caactgcagt tgaggaaaag aaaaaagaag
    12361 gtttttggtc cacaaaaatc accggcgtaa gtttgccact ttatttgatc atggtccttg
    12421 tcctgatcat tgcaatggct ttagggaaat taccgacgaa tatgattgga ccaatcgcag
    12481 ttttggttat tttcggtaat ttatttcatt ttatcgggac aaaaatccct atcgtcaaaa
    12541 gttatttggg tggtggttcc gtttttgcga tcttcgcttc tgctgcgatt gctacatttg
    12601 ggatcttacc tgaatatgta gtgaaatcca cagaaaactt tgtcagcaat atgggctttt
    12661 gggactttta tattgctgcc ttgattactg gtagcatcct tggtatgaac cgtaatctat
    12721 tgatgaaagc ttctgttcgt tttattcccg ttgcacttat ttcaatggtc gtctcctttt
    12781 ttgccgtagg actcgtgggc atgcttatcg gaaatggttt tgcaaattcc gttttataca
    12841 tctcattacc tgcgatggct ggtggtgttg gagccggagc cgttccttta tcaacgattt
    12901 atgcaaatgt tttaggtacg gatgcttctt ctatcatttc ccgcctgatt cctgcgacag
    12961 caatgacaaa tgtcttagct atcatcggtg cagcattagt tgctcgtgct ggacaatcaa
    13021 tgccaaaatt caacggcaat ggtaaattaa tggaaaaagg cgatcttggc gatggaaaac
    13081 ctcgaactgt gaaaccttcc attccacagt taggtgttgg tttgatggta tcattgactt
    13141 tcttcatctt aggacaaatt ggtaactatt ttgttcctaa agtccatgct tacgcattta
    13201 tgattattat cgttgtcatc tgtaaaatcg ccaacctatt accagagtat tatgaagatg
    13261 cagctatcat gttcaataac ttgattgtca aaaacttgac agcagctgtt cttgccggta
    13321 tcggtattgc gttgctgaat ctgaatgtat tagcttctgc tttgacatgg caatttgttg
    13381 tcttatgtct gacaagtgtt atcgcgattt cagttgtttc aggatttgtc ggtcgtttgt
    13441 ttggtttata tccaattgaa tcaactatta ctgctggctt atgcaacaac tcgatgggcg
    13501 gaacaggaaa tgtagccgtc ttatctgcag ctaatcggat ggaacttatt gcttttgctc
    13561 aaatgggcaa tcgacttggt ggtgccatcg ttttgatcat ctctggtttc ttgatgcaat
    13621 tactttctta gtcaaaaaaa caaaaatacc tgatcatcaa gtatcggctt ggtgatcggg
    13681 tatttttttg ataaagattt acaggatttt caaaaatctt ttttgcattt tatcggaatg
    13741 ataaccgatg tgttcataaa attggtgtgc ttctactctg tattctgcag aattaagcct
    13801 tattgtcgtt attcctcgtt ttttcgcttc ttcttctacg ccagtcatca aagcctttcc
    13861 gatgcctctt ttttcagcat atttagacac agcaagtgcc ataatattga acattggatc
    13921 gctaaaaatc gattcatagg actccgcttg tacatagcca gctgcttttt tagaagtagg
    13981 atcttcataa accaagaaaa aatttttctc tggctccttc agcagtttct tcatttgtct
    14041 caccgtaacg gttaatggta tatcataccc taattgatcc gcattgattt cacgtagtgc
    14101 ttctgcatct tctaaacgca tttcccgtaa catccaaatc accagccaat ttctgatttt
    14161 ttaaaatgaa tttttttatc atagtatact ctttttttca tttagaacaa tatgtttcag
    14221 tgattttgac ttgcgttagt ctagattctc ctaatgtttg taagatgaca aaggcattgt
    14281 aacaattgac caaaagaacc acgaggacaa ttacttgaaa aataaaggtc actttttttg
    14341 gagacaaaac cctgctaaat cttgctcccc acaaacctcc aataacagca gcggggataa
    14401 cataaaacaa catagataaa tcaaagcgca taaagctact tgttaaagca atcgttacca
    14461 attttgccag ttgagaaaag aaaatcgtag atagagaata tagtgttgct tcttttattg
    14521 gcaatgcaaa catcagcatc aacaaagaaa cattgactgg tccaccacca atacctaaaa
    14581 aactagctaa aaagcccaga acccccccac atataaggta ccaagtccat gacgtcaaat
    14641 gaaattttgg ctgatgatgc ttggtgtaga agaacgcaaa aaccaaggtc aataccgtca
    14701 aaaaaatttg gatcagctgc acatgcttct cgttttcaaa taactgtaaa aaaacttcga
    14761 atacaatgtt gccgactact cctccaacaa cagctccact agatacccaa ccgacaatct
    14821 gccaattcag tgatttcccg cttgatactt gtcgcacagt cgaaacaatg gacatcgtga
    14881 ataccgcaac tgttgagtaa aaagaaatcc ctgcaacaga atgggcgccg ataagatcaa
    14941 gaatcggttt gatcaacacg cctccaccca tcccggaaat agcgccaact gtgttcgcca
    15001 gaacgatcac cacaaaataa atcagaccaa tcatcttgta tttcctttct cttgtttata
    15061 tcttttagcg attgttactc ttttctttta ttatacagta aaagaaacaa acgaggcttc
    15121 gaaagctttt ctacttttta aggaaattta gtgctaaacg aaaagaggtt gtgaaaaagc
    15181 tgtcctgcat caagtaataa gaacagccaa acaaaaatag ctcctcataa ttttcgtttg
    15241 gttgaactta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga ggtcgtgagc
    15301 ctgccgttca gctccgagtc acaaggaaca atttcaaaaa acagcttctc atgtttgttg
    15361 aatattgtga cttgtgaccg aggagttggc aggagaacac cgtttaagga agagactgtg
    15421 aagtcaacgt tttgacctga ttattagaga aacaaatggc aaaatagttc ttcatatttc
    15481 gccgtttttc gagcgaatgc cgaggggcag ctgattgaac atcgtttatt tggaaaaagg
    15541 gattgtgaca agacgtttgt cacaatccct tt
//
LOCUS       DAZUMV010000057        15207 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000057 DAZUMV010000000
VERSION     DAZUMV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.057"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..2250
                     /locus_tag="ACG8FK_001584"
     CDS             208..2250
                     /locus_tag="ACG8FK_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5097194.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="ACG8FK_001585"
     CDS             2261..2881
                     /locus_tag="ACG8FK_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS5097195.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="ACG8FK_001586"
     CDS             2893..3351
                     /locus_tag="ACG8FK_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5097196.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="ACG8FK_001587"
     CDS             3370..3648
                     /locus_tag="ACG8FK_001587"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5097197.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="ACG8FK_001588"
     CDS             3673..5040
                     /locus_tag="ACG8FK_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5097198.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="ACG8FK_001589"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="ACG8FK_001589"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFS5097199.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="ACG8FK_001590"
     CDS             6227..6718
                     /locus_tag="ACG8FK_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097200.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7161..8360
                     /locus_tag="ACG8FK_001591"
     CDS             7161..8360
                     /locus_tag="ACG8FK_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5097201.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8855..9613)
                     /locus_tag="ACG8FK_001592"
     CDS             complement(8855..9613)
                     /locus_tag="ACG8FK_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5097202.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9781..10716
                     /gene="pfkB"
                     /locus_tag="ACG8FK_001593"
     CDS             9781..10716
                     /gene="pfkB"
                     /locus_tag="ACG8FK_001593"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5097203.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10713..12158
                     /locus_tag="ACG8FK_001594"
     CDS             10713..12158
                     /locus_tag="ACG8FK_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5097204.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12186..12641
                     /locus_tag="ACG8FK_001595"
     CDS             12186..12641
                     /locus_tag="ACG8FK_001595"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5097205.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12660..13634
                     /gene="lacD"
                     /locus_tag="ACG8FK_001596"
     CDS             12660..13634
                     /gene="lacD"
                     /locus_tag="ACG8FK_001596"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5097206.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13745..14306)
                     /locus_tag="ACG8FK_001597"
                     /pseudo
     CDS             complement(13745..14306)
                     /locus_tag="ACG8FK_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14450..15064
                     /locus_tag="ACG8FK_001598"
     CDS             14450..15064
                     /locus_tag="ACG8FK_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5097207.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
BASE COUNT         4755 a         2486 c         3236 g         4730 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg attactcgat ccctgctttt tttaacttcc tttgcagcaa aaaaagctcg
     6901 taatctaaga gatgatggat agtttttaga atatatatat tttcttcaat tccagaaaaa
     6961 aatgaccaag ctagtatgct aaaaaagtac gatgcttcta tgatcttttt tccgtcttta
     7021 ttaacagatg ttttaattga tcaacgcata ttgtaatcta ccctaatttc catagttctt
     7081 caccactttt aaaaatagta aaacccctac aagtatgcta tagtaaattg ctaaagaaaa
     7141 accaataagg tgggagatgt gtgcgcgatc aaacaagcgg aaaaatgatt cgtttttatt
     7201 atacaagttt gcattttctt tattggatga tgttttgttc tgtctatggg tttgcgacat
     7261 tgtttttgat ttatttcaag attgatccag ctaaaatcgg agggatctta gcattggtca
     7321 atattgtttc aacggtgctt cagccttttg tcagtcaagt gatcatcaag aaaagaaaga
     7381 tcccactgat caccgtgctg aagacgatga tagcaggatt aggagtgatt ctcttagctg
     7441 gggtattgat tccgaatttg ccgatcatta cttatatctt gggtgggatt ttagtagttt
     7501 ctatgcaatc atttctcaat gcgctggcat ttgagtattc aaataccggc tacaggatca
     7561 actttggctt ttcaagagct ggtggttcat tttcttatgc tgtaacttct tttttactgg
     7621 gacagctgtt agcgatttat tctgccgcga ttttgccaat cttggttata gttgaagcag
     7681 tgatattttt cgttattttg catctgctgc cggatgtaaa aaatcaagaa gtttttccta
     7741 tgacaaaaga acaggatcgc tcagtttctt tgagaaaaaa atatcctttc ctgattttgt
     7801 tatttttagg tttcagtttt ctttttactt tccacaccat gatcaacagc tttttggcac
     7861 agatctttga aaatatccac ggcggcagta aagaagttgg catcgcattg atgatcgccg
     7921 ctttatgcga attaccggga atgatctttt ttgaacagct tgtcaaaaag aaatccagca
     7981 aattttggct gtctgttgcc agtatttcat ttgctgtcag aggattcatg atgctgctgg
     8041 ccagttcgat cctgatgatg gaaatgacgc atctcctaca agccttcagt ttcgctttgt
     8101 ttattccagc ttcggcttat ttgttcaatc aatttctggc ggatgatgat cgtgtatttg
     8161 gacaaacgat gatcatcatc gccacaactc ttggtggggt gataggaaat gtcttgggcg
     8221 gcagtctgct gcagcatttc ggtgtatggg agatgctgat tttcggtact ggttttgcgg
     8281 tgctaggtgc tttattaact attttgggca ttcgaaaaat accgaagaca ggaaacgata
     8341 gtgattctct tgttgagtag cataaaaata cttagatact agttttatat aaataacata
     8401 tagtgattat tggcgtaatt acaaaaaacg agaataactt aagtaagcat ttctaaataa
     8461 ttgagaaacc tcatctaaaa agataagaaa atatccctaa taaaacttct gtaacatttt
     8521 agataactga ttgtaatctg aataataaac tataaggaga cggttaggcg gtctcctttt
     8581 tttgcgtatt ataattctag aattgttgga aattgataat aattaacaag aaatattaga
     8641 caggaacttc gaactgctta tcacattctt attattatat atttgcgtaa gtagtcctgg
     8701 tagtctcaat gcactattct atcttgaaac taagaagtta tagaaggagc aatgatagaa
     8761 agcacgagaa tacaaatagc gattaaatat ttttctagac aaccttagtt aattcacaca
     8821 gatatatttt aggtacagtc ttacagatat agagttagtg ctgtgtgaat cctgatgttc
     8881 gcagtgcata ggagtcaagt aagattctag ccagtataga aagtggaagt ctggagcgta
     8941 cctcataatc tgggaagaag gagccttctg atttaaagcc aacaaaagga atctcgatat
     9001 gttccatcaa acttgaatga cgattagcag taagcaaaat tgtcccgatc tcagctttat
     9061 aacagttttc agctgcggta gtcaattcct ttgtttcacc gtttagtgaa acaaagatca
     9121 cgatgtcatc tttggataat tttctactaa tgttttttat gatattggga tcagtatgca
     9181 tctcacaata tttaccggta agttgaaatt tgaccatcat ttcctgagca atcaattctg
     9241 aaaaacctcg ggcaaaaatc atgattcttc tagcagcctt gattcgttga atagcatctt
     9301 caatattccc gctttctacc atattgatgg tgcggattac ctcttgctca tttttaagaa
     9361 tagcagtctt tattccttca tcaactttat caagagcggc aaaattgata tttgtatttt
     9421 ctttttcttt taacgaatgt ttaaaagacg taaacccctc atatcctttt ttcttcatag
     9481 tacgaacgac ggttgctgtt gaaacgttgg catattcact tagttttaca atggaatagg
     9541 aggggatttc gagtatgtga ctttgaataa aatcccagag atatttctcc gattcactta
     9601 attttgtatc catattttgc ctccgattag ttaggataat tctattgtaa agcgggagtt
     9661 tcctttactt caagtttatt taatatattt tgtaaaagtt ttcaatgaat ttagtcattt
     9721 atgaaaacat ttacattcga tcttgataca taatgaatat gtaagaaagg aggagcgtaa
     9781 atgtcagttt atacatgtac tttaaacttg gcaatcgacc tttttatcga aacagatgaa
     9841 atgaaaccat cccaagtcaa tcgaacaaac gatgatgata tacaagctaa tggaaaaggt
     9901 gtgaatgttt cattgatcat gaagcaatta ggtatcgata gtacagcttt aggatttaaa
     9961 gcaggtttta ccgggaatta tattgaggat tatttgcatc aaaaagatat tagcactgat
    10021 tttattagcg taccgggaat gactagaatc aacgttttta cacaagttaa taaaaccaat
    10081 gaagaattca aattggttaa taaaggacct gagattcctc aggaacaaat caaaactttt
    10141 ctaaatcaaa tacaaaagct gcaagctgac gattatttgt gtgtttctgg cagtttgccg
    10201 cgaggggtct cacccgaaat tcttattgat atcagtcgta tttgttttga aaataaggtc
    10261 catctgatta ttgattcaag ctatccggaa ataattgatt gtctgccgta tcagccgttc
    10321 ttattaaaac caaacgagga ggaattgcaa tcttggtata atgtcgagct tagtgatcgt
    10381 gaaggatata ttcattatgc tgaacgcttg attgccgatg gagcgaaaaa tgttctcctt
    10441 tctctaggag gagagggagg aatattttta aataaggata tcgtttattc tgggaattct
    10501 cctaaaggta aagtagtcaa tacagcgtgt gctggtgaca ctttgttggg aacatttctt
    10561 gcaggatatt taaaaggtga acaggtagat attaatttga agaaaagttt agcagctgga
    10621 agttcaaccg cttttcgtaa aggattaaca gatttttcgg atgttgcaga gttggaaaaa
    10681 caaatcgaaa tcaaagaggg agcgagtaaa aaatgacaac ttacaaaatt gttgccgcaa
    10741 caggatgtcc aacaggaatt gcccacactt atatggctca agaagcattg gagcaggcag
    10801 ctaagcgcaa aggggtatct atcaaagttg aaacccatgg tcaagtaggg atagagaatg
    10861 aattaacacc agaagaaata aaaaacgctg acgctgtaat cattgcagct gacaaagatg
    10921 ttcaagcaga acgtttcgca ggtaagcgtg taatcgatgt gcctgtcagc aaggggatta
    10981 aagatgctga ccaattgatc gaagatgcgt tggctggtaa aggttcaatc actgcagaaa
    11041 acaaaacagt tgacgctgct ggtgagaaag cggagatggg ggttgcagca ggcattggtc
    11101 acagtattta taaaaatctc atgaacggtg tttctcatat gctgccgttt gtagtggcag
    11161 gtggggtaat tattgcaatt tcgtttgcca tctggggtgt gtactctttt gatcccaatg
    11221 atcctactta caatccgact gccgctatgt taaagggtgt tggtgatgcg gcaatgggaa
    11281 tgatggttcc tattttagca gcttatatcg ctgaaggaat cgctaaacgt cctggtttgg
    11341 ttgttgggtt tgttggcgga ctgttagcca attctggcgg agccggtttc ttaggcggaa
    11401 tttttgcagg tttcttagca ggctacttta tcttactatt gcaaaaggtc atgaaaggct
    11461 tgcctaaaca attggatggt ctgaaggcga tcttcttata tccggtgatc ggtgtcttgt
    11521 ttgttggtgt agtcatgtct ttattagcaa atccaatgga agcagtcaac caaggaatga
    11581 tgaatttctt ggcaggattc caaaattcta atccattaat tttagggatt atcgttggct
    11641 gtatgtcagc atttgatatg ggaggtccag tcaataaagc ggcttatgtt acagggactg
    11701 cgttgttggc acaaggtaac catacattta tggcaggggt ttcggcagct tgtatcgcgc
    11761 ccccattgat tactggattt gccgcactgt tttttggaaa atatttcgac acgaatgatc
    11821 gcaacgctgg tttagtcaac tttatcttgg gctcaacgca cattaccgaa ggagcaattc
    11881 catttgcggc taaagatccg ctgcgtgttc tgccaactat gatgctaggt tcatcaattg
    11941 ctgctgtact gacatatatg tttggtgtcc aagttcccgc acctcatgga ggttttctag
    12001 tattacctgt tgttactggt aaggttgcat gggtagggtc gattttactt ggttcaatcg
    12061 ttggcggagt aattttaggg ttgatccaaa aacgtagtgt agaaaaacgt gaagcagaag
    12121 cagctgctgt tgaaatgaaa gaagcaacca acaactaaag gttattctag aaagaaggat
    12181 ttcaaatgac tttgatcaaa acaaataaag tttttatgaa taaaaatttt acgacgcaag
    12241 atgaggtaat gaacttcctt gcagatcagt caattgtctt gagcattagt gaagaccgag
    12301 atgcagttta cgaaaaattg atggaaagag aagaagaaag cacgactggt atgatggatg
    12361 gttttgccat ccctcatgcc aaagatgcta gtatcaagga tgccgatatt gtgattgttc
    12421 ggttaaacgg taaggtcgat tggaaaagtt tagatggaga aggaacggat tttgtaatcg
    12481 ctctgtttat tccagattca gaggctggaa caacccatct aaaattttta tctcaggtag
    12541 ccagactttt gatgcacaaa gatgtgacag aaggtctgaa acaagcccag tctgctgaag
    12601 aaatagctga acttttaaac caaaaattag aagaaaacta aaaaggaagt agtgaaatca
    12661 tgcctttaac agaaaaaaag aaaacttata ttcgtcaact aagtaatgaa agtggcatca
    12721 tcagtgcgtt agcaatcgat caacgtggtg ccttgaaaaa aatgatcggt aaatttcaaa
    12781 ataaatcagc aaccgatgaa caaatcatca atttcaaaaa aatcatttct tctgaattga
    12841 ctccatatgc ttcagcaatc ttattagatc cagaatatgg gttgccggca gccgaagttc
    12901 gcgataaaaa tgctggtctg ttgttggcct atgaaaaaac tggttacgat gcttcaactc
    12961 caggacgttt accggatatt ttatctattt ggtctgtaaa acgtctaaag gagcaagggg
    13021 ccgatgcttg taagttcttg ttatactacg atgtagatga atctgatgac atcaatgacc
    13081 aaaagaaggc gtttatggag cggattggtt ctgaatgtgt tgctgaagat ttgccatttt
    13141 tcttagaatt ggtttcttat gatgccaacg atcctgatac ttcttcgaag gaatatgcct
    13201 tgaaaaaacc gcataaagta aatgaaatga tgaaggaatt ttctaagcct cgctatggag
    13261 ttgacgtttt gaaagtggaa gttcctgtta atatgaacta tgtggatggt tacactaaaa
    13321 atgaagttgc ttataccaaa gaagaagctg ctgcttattt caaagaacaa tccgagacta
    13381 ccaacctgcc atttattttc ttgagcgcag gagtaactgc tgaattgttc caagaaacct
    13441 tgaaatttgc aaaagaggct ggctctacat tcaatggggt actctgtggg cgcgcaactt
    13501 gggcgaatgg tgtagaacca ttcgttacta aaggtgaata tgctgccaaa gaatggatgc
    13561 agacacaagg acgcaagaac atcgaagagc tgaataatgt tttgaaagaa acagcaacag
    13621 ctattaaatt gtaagaaagc tttaactata accttaagtt tgtaacctaa tgatttttaa
    13681 taatcaattt gagcttttaa ataaagaatt ggaagcgtcc cgacgcttcc tttttcgttt
    13741 gtttttaagg catgataggt tctatgttgt cgttcaataa gcgcaagaag gtcagcattt
    13801 ttttcttaga gtatgcaaaa ttgcttttct acccaatgat ttaaaacgga gaggcaccct
    13861 tggatatggt agatggtgaa gtaacgcatt tcttctgtga tcacggtttt tttgtcttct
    13921 tgtagccaga tttttaaata attttcttcg atcagattat ttaatctctt ttgaaattgg
    13981 atatctccgt ggttgatgac gaataacttg tatacgaacg tattcgtata gacatagtcg
    14041 atcaattgag cgtatagatc ctcgtagcta tgtgtcgtat cgtaggaaat gatttcatcc
    14101 aagtcttgga aaaagctatt ttccagtttt tcataaagat catagatatc cgtataatgt
    14161 gtataaaatg tcgaacggtg aagatcagca gtttcggtca gttcacggat ggtgatgttt
    14221 tgaatttttt tatctactaa taattttgat aatgcttctg ctaacgcttt ttctgttttt
    14281 ttgacgcggc gatcctcttt tttcattttg cagcctccta atcaacataa tcaaggaatt
    14341 gtgtcggtta tccgacaatt tcaccgctat ctgaacatag ataaccgaca ccttgtctat
    14401 tataatataa gtatagcata tcaatattta aagaatagga gaataagtga tggaattagc
    14461 ctttaagcaa ctggaaaaga aagactacaa aaaagccatt caattcgcga ttaaaggtat
    14521 gcactttgat tggtatacca agaacaaact ccttttgaat ctttatggaa gatacttttg
    14581 gtatctagaa ttgaatcgcg cgacacaaat cattgcttta tatgcggatg atgagttggc
    14641 aggtgtttta ttagcagaca tcaaaggaaa atcaaaaaag catcattcat ttagtcaaaa
    14701 gttatatgtt aaactatttg atttcttaca aaatgcattt gtgaaagaca gcaaaggcgg
    14761 gtatgacgac accaatgacg aattgctttc agactattta aaaaaacatt ccccagatgg
    14821 agaaattgtc tttttagctg ccaaccctga tttaaagatc aaggggatcg gtagtaaatt
    14881 attaaaagaa tttgaacgcc gagagcaagg aaaagaagta tttctgttca cggatagcgg
    14941 ctgtacgtat caattttatg aacgacgcgg tttcgaacgg aaaaaagaaa aagataacgt
    15001 catgaagatc cttaataaag aagtagatct ccgctgtatg ttatatagta agatcattca
    15061 gtaatgtttt ttactatcag aaagcagaag taccacgaag gtcaatggaa aaagcgagta
    15121 attgatccat ttagatgaag ataggtgtag tctcaaagat attagctggc aaagatatcc
    15181 ccagttaaaa atagatttct gtatcca
//
LOCUS       DAZUMV010000058        15200 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000058 DAZUMV010000000
VERSION     DAZUMV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.058"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            464..1279
                     /gene="thiD"
                     /locus_tag="ACG8FK_001599"
     CDS             464..1279
                     /gene="thiD"
                     /locus_tag="ACG8FK_001599"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS5097208.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1608..3059)
                     /gene="cls"
                     /locus_tag="ACG8FK_001600"
     CDS             complement(1608..3059)
                     /gene="cls"
                     /locus_tag="ACG8FK_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5097209.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3400..6066
                     /locus_tag="ACG8FK_001601"
     CDS             3400..6066
                     /locus_tag="ACG8FK_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5097210.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6430..7182
                     /locus_tag="ACG8FK_001602"
     CDS             6430..7182
                     /locus_tag="ACG8FK_001602"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5097211.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7204..8358)
                     /locus_tag="ACG8FK_001603"
     CDS             complement(7204..8358)
                     /locus_tag="ACG8FK_001603"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS5097212.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8543..10984
                     /locus_tag="ACG8FK_001604"
     CDS             8543..10984
                     /locus_tag="ACG8FK_001604"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS5097213.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11314..12018)
                     /locus_tag="ACG8FK_001605"
     CDS             complement(11314..12018)
                     /locus_tag="ACG8FK_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097214.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(12205..12450)
                     /locus_tag="ACG8FK_001606"
     CDS             complement(12205..12450)
                     /locus_tag="ACG8FK_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097215.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12467..13318)
                     /locus_tag="ACG8FK_001607"
     CDS             complement(12467..13318)
                     /locus_tag="ACG8FK_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5097216.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13484..14488
                     /locus_tag="ACG8FK_001608"
     CDS             13484..14488
                     /locus_tag="ACG8FK_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS5097217.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(14729..15085)
                     /locus_tag="ACG8FK_001609"
     CDS             complement(14729..15085)
                     /locus_tag="ACG8FK_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5097218.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4856 a         2689 c         3133 g         4522 t
ORIGIN      
        1 tgggtatcgt aaccttcttc atgtaggaaa tagattattc ctttttctgt gttttgcgta
       61 taagctttta tactcctgtc ttttttgaaa gaacagatcg tatagccatc tatttggtct
      121 ggtagtcgtt tgattttctt caaaatgact tttttatcct caagtttcat ttttttcacc
      181 tgactttatt tttttcatat ggaaatcata gcgcctttgg ttatatgaaa acaagaagga
      241 tactgaaata tatataaatc aaatagtggt tatttattat tagaatagcg aatacaaata
      301 aacgaccact attttctgat ttattgcaaa tgttttttca aataaatttg attaccaaga
      361 aaaggtagtg gtatatttac tgagggatac tttgaaaaac atatgataga aacagtaaca
      421 gtattatgaa aaatcttttt ataaaaatgg aggaatcgat ttgatggaag atcagtatgt
      481 acaagcaatg acgatcgcag gatcagatag cgatggaagc gcagggatgc aagcagatat
      541 gcatacattc tttacaagaa atgtttacgg tgtttctgtc atgaccgcat gtgttgcagg
      601 taattcttac ggaatcggag caagtgtgac attgccaaca gattttatcg acaaagaatt
      661 tgaactgatt gcgaaggact ttcaaatacg tgcagctaaa acaggtatgc tagcagattc
      721 aaagttgatt gagacagttg taaaaaatta ccaaaaatat gattttggac cattagtcgt
      781 ggatcccgta atcgtaacga aacatggaaa tcttttacta gaagaatctg ctttgcaaag
      841 cttgaaagaa aagcttgtcc ctttggccga agtactgaca cctaattttt atgaagcaga
      901 aaagctgact ggacggacat tagcagctga agaagatttt gtgcaagcag caaaagaact
      961 gcaagatatg ggtgcaaaaa acgttatgat caaaggacat catgctaaaa atgcgcagca
     1021 agaaacagtc aatgactacg tattgttcga atcaggaaaa tcattttggt taaatgcacc
     1081 attttatcct accgaccgga tcaacggaac tggcgattcg ctatctgcat gtattgcagc
     1141 tgagttagcg aaaggaaaaa cagttgaaca agcgatccga ttggcaaaag actatgtaaa
     1201 ccgtgcaatt cgtgaagaaa taaatgtagg gcacaaatat ggccccatca atcattgggc
     1261 agctggtgaa ttagagtaaa agaaagaagt tgtgaaaaag ttgccctgca tcaagtaata
     1321 agaacagcca agtaaaaaca gcttcttatg tttttaactt ggttggactt attaccgcag
     1381 atgcaagctt ttgaacaccg tttaagaaag aggtcgtgag cctgccgtcc agctccgagt
     1441 cacaaggaac aagttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc
     1501 gaggagttgg caggagaaca ccgtttgttc ggaagaaggg actgtgacaa gatttttgtc
     1561 acagtccctt taatcttagt atatttttct ctacttgaat aaaaaaatta taggattgga
     1621 gaaaataatc gagaaatttg ttgcttgaat aatagccagt tagacatttc cttgactgtt
     1681 tcagttgtca tggatgaaca atgtgtctca tcttctttga actgagtagc aagttcttct
     1741 aaaaactcag gatcgtaaat gacagcactt gtttcaaaat tcaatcggta gctacgaata
     1801 tcttgatttg ctgaacctac catgcagatt tcatcatcca ttatcaatgt ttttgcatgg
     1861 aggaagccgt tctcatagac aaggatttgt acattttcct tgatcagctg ccgagcataa
     1921 tattgtgttg ctcgataaat aaaaggatga tccggtttgt tgggaatcat gattttcaca
     1981 tctacaccag aggcagttgc gatttttaaa gcagcaatga cactttcatc aggaacaagg
     2041 taaggtgtct gtatccaaac acgtttttta gcagaagtaa tcaatttgat aaatgaaagc
     2101 ttgatttgtt ccctttcgtt attcggtccg cttgaaacaa gctggatgga tgtatcagca
     2161 agcttagatt catctcgatc tgctttttta aagaaataat tcaaatgata gccgaccttt
     2221 ttttcttcag gtaccgagac gttccagtct gttaagaagc gaagctggag caaagaagaa
     2281 gcggcaccga aaatccgtat atgcgtatcg cgccaatagc cgaatttttt tgttgtattt
     2341 acatattgat tggcaatatt gaaaccaccg gtataactaa tctttccatc gatcacaacg
     2401 atttttcggt gatcatggta attcaatcgg aaacgaagta atgccctttg tgaggtaaca
     2461 aatgtatgga catgtccacc atttttgatt agacgattga aatcttttgc ttttgtgcca
     2521 tgagagccaa atgcatcata taatattcga acttccacgc cttctgcggc tttttcttct
     2581 aaaacatgta agacttgctg gccgatatta tctgtcacaa atgcgtaata ttcgatatgg
     2641 atcgaatgtt tggctttttt tagatcttga agcagtgaat ccaatttctc ttgtccgtct
     2701 gtgagaagag tgacagaatt cattcttgtc agcggcatac gatttaatga agagaagaag
     2761 tcaacaaatt gctgtttgtc tttatcgccc atgtcaggat cgtaatgttc aatggtatct
     2821 cctctaaaag actgaaagtt ttctaattct ttcaagtcac tttgtcggag atagaatttt
     2881 ttcttatccg ttaatccacg tccgaaaaat aaatataata caaagcccac cccaggaagg
     2941 gcaaacaata ctaagagcca tgcccaaatg gctgctacat ctcggggttt gatcaaaata
     3001 gccaccaaag caataagtgc atttaataga taaagggcgg ttataatact agataccaca
     3061 aaaatgttcc tccttacata caataacatt agtatagcat gatagaagaa agaagcctac
     3121 caaggtgaat atacttttca ctcctttcat gaaaatgtag gtaaatcggg ctaaagtagt
     3181 tgctttttgt aggttaataa ggtaggatat tagtacagtt gaatatctcc ttaggggagt
     3241 aaccggcaac gtttgttgcg ataatatcaa cagtaagtta accaaatggt tttctggtat
     3301 tgtatgaaat ggtgagacct aagacaaatg cgtatttgtc ttaggtctct tttttttcag
     3361 acaggactag ggccaagatc aaaaaatggg ggatgaaatt tggaaaacaa ggaacaagca
     3421 caacaagcgg tctataaaga aacgatcgac tcgttgatgc agcaaacgca gagctcaacc
     3481 gatggattga gcaccacgca agcgaaggaa aaattagaaa gagatggctt gaaccagcta
     3541 aaagaaaagc cgaagaagcc aacttggaaa ttgttcttag aaactttcaa agatgcaatg
     3601 gttatcgtat tgctgattgt cgcactgatt cagatggtca tgggtgacta catcgaattc
     3661 ttagttattt ttgcagtatt gatgttgaat tctataatca gtgttgtcca aacaaaaaaa
     3721 gcagaaggtt ctttagaagc gttgaaaaac ctttcagcgc ccgatgctcg agtgattcgt
     3781 gatggtaagg aaaaaacgat tccggcaaac gaacttgtgg taggagatat cgtgttgtta
     3841 gacgcaggcg attatgtacc tgctgatgga cgtttactag atgcgggatc attaaaagta
     3901 gatgaaggta tgctgacagg agagtcgact tctgcagaaa aagaagtaac ggatatcaca
     3961 agtacggtac caatcggaga tcgtacaaat atggtattca gtggaacgat cgtaacttac
     4021 ggacgcggta aattcttagt gacttcaact gggaataata cggagatcgg acaagttgct
     4081 ggccttttgg aatcaacaac tgagcaagtc actccgttac aacgaggtct ggataaattc
     4141 agtaaaaaac taagtttggc tattttggca ttgtctttat tgattttagg tatccaactg
     4201 tttcgtattt atcttggaga aggtacagga gatatgactg cagatatcgt cagcgcagtg
     4261 atgtttgccg tagccgtagc cgtagctgct attccagaag cattacagtc tatcgtaaca
     4321 atcgtgttat ctttgggaac gaataaaatg gcaaaacgtc atgccattat tcggaagctt
     4381 ccagcagttg aaacacttgg ttcgacaagt atcatctgta cagataagac tggtacattg
     4441 acacaaaaca aaatgacagt tgttgactac tttttaggta atggcgatac tggggatttc
     4501 acaaatgacc cttcaaaatg gacagctgac gaacgtcgct tgatcgaaat cagtgtttta
     4561 gcgaatgatg catcgatcag tgaagacggt actaaattgg gcgaccctac ggaagtagcg
     4621 tttatcgact ttagtgaaaa attaaaccag ccttatcagg aaatccgaaa caactaccca
     4681 agacaagcag aacttccttt tgactcagat cgaaaattaa tgtctactgg acacacaatt
     4741 gataacaaaa attacttgtt tgttaaaggc ggaccagata tcgtcttcca acgcagtaaa
     4801 aaagtattgc taaatggtga agtcgttccg atgacggatg aattgatgaa aaaattccaa
     4861 gagcaaaatg aagctttttc aacacgcgca ttacgtgtac tagcttttgc ttaccgtcca
     4921 cttgataatt ttgatttatc atttgaagat gaagacgatt tgatccttgt tggtttgctt
     4981 gctatgatcg atcctccacg tgctgaagtg actcaggcgg taacagaagc aagaagtgca
     5041 ggaatcaaaa caatcatgat tactggtgac cacaaaacaa cagctgttgc tatcgcaaaa
     5101 caaatcggga tttttgaaga aggaaacctg gctttgaccg ggacggaact agatgaattg
     5161 acagaagatc aactaaacga agttttagaa aaagtcactg tttatgcacg tgtttcacct
     5221 gagaataaaa ttcgtatcgt tcgtgcttgg caaaataaag gtcatatctc agctatgaca
     5281 ggtgatggtg taaatgacgc accagcattg aaacaagcag acatcggaat tgctatggga
     5341 acgggtacag atgtggcaaa agatgcagca gcgatggtat tgacggatga taactttgct
     5401 tctattgtca gcgcagtaga agtaggacgt aatgtgtttg ataatatcaa gaaagctatt
     5461 tcttatctat ttgcaggaaa tttaggtgcg atcattgcga tcatctttgc cttagttgct
     5521 gactggtcca atccatttac agcattacaa ctgttgttca tcaatttagt caatgattca
     5581 ctgccagcaa ttgcattagg tacagaaaaa gctgaacctg ctacgatgaa ccgcccgcca
     5641 agagatccaa attcaggaat ctttacggac aaaacattga tttctgtaac gtatcgtggt
     5701 gtgctgattg ggattgctgt tatctttgct caatggcttg gcatgcagca ttcacctgaa
     5761 ttcggtgtag ccatgtcatt ttcaacattg atttggtgtc ggacattgca aacattccca
     5821 gcacgttcaa acacacaaac agctatccaa gcagggttct tctctaacag aagtgtctta
     5881 ctagctgtag ttgtatgtag cgcgctttac tgcttaacaa tgatcccagg actgaaagaa
     5941 gtcttctcta tcccatcagc atttggattg acagaagtct ttacatgtat gggaatcgca
     6001 cttgtgacaa ttgttttgat ggaaatcact aaattgatca ttgtacgagt acaaggaaat
     6061 aaataaaacg aatcaacgag gttgtgagcc tgccgtttaa ttctgagtca caaggtgcaa
     6121 tttcaacaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga agagttggca
     6181 ggagaacacc gtttaaggaa gaagttgtaa aatcagcatt ttgagctaat actgagggtc
     6241 atctgattgg acactgttta ttcggaaaga gggactttga cacgactttg gtcacagtcc
     6301 ctttttttat ttatgataga cggaaagcca atcattttat tattcatcaa aagagataga
     6361 atagataaaa gaggagaatg atttattccg aaaagtgaaa acatataaca aaaaattaga
     6421 ggaaatcaaa tgaatgaaaa agaatgggat gaatttgctg aagaatatta tgaaatccag
     6481 caagaatcgc aagtaactat tgctgaagat gtaaaaaatt atctgaaaac ggagaaagtc
     6541 ctgcctgctg aaacagcagc agacgttgct ggaggatctg gcagatttct ttccttagca
     6601 aaagaaatta atcgctatga tttgatcgat ttttcttcag gaatgctaga aaatgcaaaa
     6661 aaagaagcag aaaaaaatga gttgccaaat gtttccttta tcaaacagtc gttagaagaa
     6721 tttatccaat caaatgagac atatgaaatg gtttttacag cagcaaatcc agcattggta
     6781 catcttattc aattagaaaa attgatgaaa aagtcaagca aatggtgcgt gataatcaga
     6841 gtagtggaat caatcgatga tgttttttat cctatcgaaa agcaactagg aatgatagaa
     6901 gaagatccta atacatcgcc agttgtaatg gatcaatttg aaacatactt aattgatgaa
     6961 ggattcaaag tgaagaaaaa agattttact tattttacac aggaatcaat tactgaagaa
     7021 ttgatgaagc tatactatga cgcgtacgca aaagatcctg ttgtgaccag gtacattcgc
     7081 aaactattca gtgaaaaacg agaaatcctt agtacgacta agctgaccta tagactgctc
     7141 ctttggtccg cagataaaaa aagaaccttt cacgactcgt gaagggttct ttttatgatt
     7201 ttattatatt ttgtagtaac gaatatcatt ttcgatcttt gtaatcgaaa aaggtacatc
     7261 tgaagtatat tctgcacatt ctccgtgttc aggaagcgtc tcactgaaaa tcgtttcata
     7321 ttcgtcaaca gtgatccgtg ttcgagaatc tagcatctct tgatgagatt gtgcgaagag
     7381 atattcttga tagttttctt ccagataacc tgtaaaaaat tcacttactg caccagaacc
     7441 gtagctgaat agaccgatgc gatctcctgg ctgtagtgat ttcgaatttt ccagtagaga
     7501 agttagcccc aggtataatg aaccagtgta aagattaccg attcgtcggc tgtaacggat
     7561 gctttcttca tagcgagcca taagacgttc ctgattttct tcgtctgttt ggtctaatac
     7621 gctttgcaat gccttttttc ccatcttagt atacggaata tggaaagcaa tcgcctgata
     7681 atcttcgagt cctcgacccg acaattcttt atgtcgattc caaacttttt ggaatgattc
     7741 gatatacgta gaattagata aaggaccatc aacaacagga aattcgctat aatctggacg
     7801 ccagaaatca tagatatctt ctgtcagaaa tacgctgtcg tcttcaatcg ataaaatacg
     7861 cgggttttga gtgatcatca tcgcaacagc accgacacct tgcgtcactt caccaccgct
     7921 tgccaagccg taacgagcaa tatcacttgc tatgactagt acttttcgtt ctggatgatt
     7981 tttgacatat tctttcgcca tgtgcaaagc tgcggtgcca ccgtagcaag cctcttttac
     8041 ttcaaaagaa cgagcaaaag attggatttt cagtaaatgg tggataatta ccgcactcgc
     8101 ttttgaatga tcaatcccgg attctgttcc gacgatgacc atgtcgatta gctcacggtc
     8161 tttttctgtt acgattttgc tggcagcatt tgctcctagt gtaatgatgt cttcatttgc
     8221 acgattgact gccatttggt cttgaccaat ccctatatgg tattttgcag gatcatcccc
     8281 acggctttct gccaactctg tcatatctaa atataaatta ggaataaaaa aggaaagacg
     8341 atcaatccct attttcattt caacacctct ttacttatga taaaaaactt actttactaa
     8401 ggtatcataa aaagattaaa agaaatataa aaaagagaat atttaaggat tgttaaaatt
     8461 taatcttaga aaaaaataac tggaacaaga gctatttgtt tttacagcaa agaatggtat
     8521 atttttgtag gaggtggctt acttgaaaga agtcgttatg atagatgctg caagaacgcc
     8581 gataggaaag tatcgcggaa gtttaagtcc atttactgca gttgaattag gcacacttgt
     8641 gactaaagga ttattagata aaacaaaact gaaaaaagat aaaatcgatc aagtaatatt
     8701 tggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc agacagattg ctttgaacag
     8761 cggtctccct gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat ctggtatgaa
     8821 agcagtgatc ttagcacgcc agctgatcca attaggtgaa gcagaacttg tgatagccgg
     8881 cggaacagag agtatgtctc aagcacctat gctgaaacag tatcagtcag aaacaaatga
     8941 atatggtgaa ccaatttcca gtatggtcaa cgacggattg actgacgcat tttcaaatgc
     9001 acatatggga ttaaccgcag agaatgttgc aacacaattt tctgtgagca gagaagagca
     9061 ggatcgctat gccttgtcgt cccagttgaa agcagcacat gctgtcgaag ccggtgtatt
     9121 ttctgaggag atcatcccag tcaagatttc tgatgaagac gtgttatctg aggatgaagc
     9181 agttcgtgga aatagtacat tggaaaaact gggcacgtta cgtacagtat tctcagaaga
     9241 aggaactgta acagcaggaa atgcttcccc gttgaatgac ggtgcctctg tggtgatcct
     9301 tgcatccaaa gaatacgcag aaaataataa tctgccttat ttagcaacca tcaaagaagt
     9361 agcggaggtc ggtattgacc caagtatcat gggaatcgct ccaatcaaag ccattcaaaa
     9421 attgacagat agatcaggaa tgaatttatc aacaatcgat ctttttgaga tcaatgaagc
     9481 atttgcagct tcttctatcg tagttagcca agaactgcag ttagatgaag aaaaggtcaa
     9541 tatatatgga ggagcaatcg cattaggcca tccaattggt gcaagcggtg cacgtatctt
     9601 gactacatta gcttacggat tgcttcgtga acagaagaga tacggtattg cttcattatg
     9661 tattggtgga ggtttaggct tggcagtctt gttagaagcg aatatggagc aaacacataa
     9721 ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct gaacggcgca gtcaattaat
     9781 agaaaaaaat gtattgacac aagaaactgc gttgattttt caggaacaaa cactttctga
     9841 agagctctcc gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc caatggggat
     9901 cgcgcagaat tttcaaatca atggcaagaa aaaatggatt ccgatggcaa cagaggaacc
     9961 atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct gcgcagaaac
    10021 acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt atcaagcagt
    10081 gatcaatgca gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg aaagttatcc
    10141 ttcaatcgtg aaacgcgggg gcggtgttca agatatctct actcgggaat tcatggggag
    10201 tttccatgca tacttatcta ttgatttttt agtggatgta aaagatgcaa tgggcgctaa
    10261 catgatcaac tcgattcttg agagtgtggc aaacaagtta cgggagtggt tccctgaaga
    10321 agagattcta ttcagtattt taagtaattt tgctacagaa tcattggctt ctgcctgttg
    10381 tgaaatccca ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa ttgccaaaaa
    10441 aatccaacag gcaggggaat atgcgaagct tgacccttac cgtgcagcaa cgcacaacaa
    10501 agggatcatg aatggtatag aagctgtagt ggcagctaca ggaaatgata cacgtgcggt
    10561 tagcgcttcg atccacgcct acgcggcaag aaatggatta taccaaggat taacagactg
    10621 gcagatcaaa ggggataaac tagttggaaa actaacggtg cctttggctg ttgccacagt
    10681 aggcggagct tccaatatcc tgccaaaagc aaaagcatct ttagctatgt tagatattga
    10741 ttcagccaag gaactagcac aagtaattgc tgcagtaggg ttagctcaaa acctggctgc
    10801 tttgcgtgca ttagttacag aaggaatcca aaaaggccac atgggacttc aagctcgttc
    10861 attagcgatc tcaattgggg ctattggtga ggaaatcgag caagttgcga aaaaattacg
    10921 tgaagcagaa aagatgaacc agcagacagc tatacaaata ttggaaaaaa tccgggaaaa
    10981 ataaaaaagc aatatcttta gaagaataaa gcaagattta cagttatttt atttactgca
    11041 gatcttgctt ttttctctat cagaaaatat gtttattatt agaatttttt atagttaagg
    11101 ttaggcatct taagaaatca ggcaactcca agatatcttc aggtagaaga gaagtcgcct
    11161 aacattgtag gatcataggt gatttgttgg acaggtcaag ctgatatttg agcctgatgt
    11221 tagaaggaat ttctttttac ttgctgtaga aattattata tgactaaaaa ggctgggatt
    11281 ttaaatccca gccttttatg acaaatagaa tttttatcga tcagtaatcg ctgtcgctgc
    11341 aaattgttcg atcaaaggaa tcaaagtcaa tttgactttg tatttccgtt cgagtgcttt
    11401 ttcgataacg aactcatttt cagtagtcat agaaatagtt gctaatttac gaggttcatc
    11461 agcatttttt tcattaataa ctacttcatg ttcattttgt tctgtgtaaa atttaggata
    11521 ctcagcaacg gcttttgaaa aatcagcaaa acttaaagcg gcattcgtat cagttgagcg
    11581 tgtattttca gttgtgtccg cttttggttt ttccgttttt tcttctggca cactgactgt
    11641 ttcttctgta actggtgtcg caacagcttg cgcagttggt tcatcgtttt tcaatcgttg
    11701 atcattccag taagacacga tttcaataag aagattgtca cggaatcgtt cgaaaatccc
    11761 gttttctcct gaacgaattc tctttaatac agtattgaag ttgtcatcga ttggcacgtt
    11821 gatccgtgta tcttcatttg ttttcccagc taatccgatc gttaagattt gacggttttt
    11881 gggatcaagc atgacttgta acggcaaacc ataacgtcgc aagctctctt cttctagaga
    11941 acggaataat tgtcctctta caccatccaa tgaaggacgg aattgattta ataatttttg
    12001 tttctgtttt ttcttcatat gttctctcct tcgctcatac cctttcgatt ataagaaaaa
    12061 aacagccaaa tgaaaaggag taatgcaaaa aaacttgcgt atacgcaaat tacaacagaa
    12121 aaatttgcgt attttcccgt tttttgaaag aaaaataaaa aatggaacgg attatgtata
    12181 tatacataat ccgttccatt ctttctattt tttcggtatc aaggcaaaaa caggtagttc
    12241 aggtgaataa atgacttcat gttcgtgcca gggtttgacc attagcgaat gatcgaaccg
    12301 ttttttcagt tcttcgatga tttgatcatg tgtccagttt cttgcaggaa aatgttggat
    12361 tttctctgca tcgatcaaaa agacaaaatc ggaatgaaaa gtctgggtaa cttcttccga
    12421 gagagaattc aagcgttcga ttggatccat aaaatcacct ttcgacttaa aattgggtag
    12481 tatctgggtc ttttgctttg ccgactacac catctaactg atcgatttgt ttcatttctt
    12541 cttctgttaa ttcaaaatca aataattcag tattttcttt gattctgctt ggtgtcactg
    12601 acttaggaag aggaaggaat tcgtgttgca gcgaccaacg caaagcaact tgtgcaatcg
    12661 tcttgtcatg tgcttcagcg atttgtttca tttcaggcac atcgaagatt ttaccagtgc
    12721 ctaatggact atatgcttcg aggatcatat catgtttttt tgcatactca actacagttg
    12781 tctgtaattc gcctggtgca aggaaaactt gattgaccat cggcataatt tttgctgttt
    12841 ttgctagtgt atctagatgg tggggtaaga aattgctcac accgattgct ttgattttac
    12901 ctgcttcata cagttcttca aatgctcgcc atgtatctgc atttgcttgt tcccaattat
    12961 cacgaaaagc aacagggttt ggccagtgga tcaaaaacag atctaaataa tctgtttgga
    13021 gcttcttcaa tgattcttca aaagaagaca tcacaagttc atagctgtgg tttgcattcc
    13081 ataattttgt tgtcaagaat atctcttctc taggaattcc actttcctta atcgcttggc
    13141 cgacgctttc ttcattcttg tatccttggg cagtatcgat atggcgataa cctgcagcca
    13201 gcgcttcttt aacagaggaa acagcgacat ccccatcagg tgtttgccat gtgccgaatc
    13261 ctactaccgg gatttcgtaa ccatttgata gacgataagt gcttgttaat gaattcatac
    13321 tttcagctcc ttttttattc gttcaactat aagtgtagcg catttttcga aaaaaagaat
    13381 ccattttgtt tctactcttg aaatttttgt ttaaaagaaa atgattttga ttctggtatt
    13441 tttgattttt tttccgtaaa ctaagacaga agggaatgag aaaatggata tctatttaaa
    13501 aaaagcaatt ctgcatatta ttgacagaga aactggttct ccagtctttt cacaaaaaga
    13561 gctggatctg acaaaagaat atattcgtga ttttcttcaa aagaagatcc aaaaaatctc
    13621 ttctgctcaa acaaaaactg gacaactagc agaagaaagc atgatcgttg aatcacttaa
    13681 aatcgctcaa gaaaattttg tggaagcaag tgaagcaatc gttcagcggt ggttcgatat
    13741 ctaccaagaa agtgaagaag cacctagtgc agatgtattt gttgtcttgt atgaattaga
    13801 tacagtgatg tacttatctt tattgaaagt caattatcgt gaggcttaca ctcactttgt
    13861 tgaagcagac gaagcaggga tcgacaaccg cttgattttg aatcgagcaa ttctaggggc
    13921 gaaaacacaa aaagcagatg aagcattcgc tgtgaatttg actgattgga cctacgaatt
    13981 aattgaaaaa aaatatgagt tttctggcaa aaaagagctt tatttttctg gaagagtgat
    14041 cgaaagtgtt cctgcacctt ctttagaaga aaatgtaaaa gtgatccaaa aagtagccaa
    14101 gcatcttgga aaaaaatttg agacagaaga atttgatatc atggctgatt tgaaagaagc
    14161 tgtctatgat acgatagaag aaaaaggtcg gttggatcat gagatgattg cggaaaaagt
    14221 cttcaaggaa aacatcacgg caaaaatggc ttttaaagaa gaagtacaag agcaaggatt
    14281 tgtcccagaa gcacctcctg taaaagaagt acaagaaatt tctgagaaaa aatttggaaa
    14341 acaaaaattc aagctatcaa acgggatcga actgatcgta ccagtagatg tatatcgcaa
    14401 cccagatttg attgaatttg tgaataatcc ggatggtacg atatccgtga tgatcaaaaa
    14461 tgtggatgaa gtattgaatc gtttataaaa atgactagaa tattaggtgt tcaagtacca
    14521 gtgatcagtt aaatgaattg tgtcaagaga aggatcggaa aagacgaata taacaaaaaa
    14581 agagcgtgag acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
    14641 ggaagcagaa gcaactcctt cttatttctt ggagttaaac gcttctgtcc cagcctcttt
    14701 ttatctaata aaattttaag accatttact aattatcgat tttttctgcc gcatttttag
    14761 gcacttcaga agcctttatt tcttcccagc ttaatacacc tttacgagga ttgactttga
    14821 gtttaagata cgcattcatc tttaatggat gttctctttc ttcgcgaagc gtcacctgtt
    14881 ttttttcacc atctttatta taagccgctt gcttgtaggt ataagtcgtg aatttctctc
    14941 cgttatctgc ttgggtagct gacatttcgc cattcgtcgt gatttttgta taataagctt
    15001 cgccgccgta aaaataatga taagctgcaa aacttccgcc gagaaatatg cctaaaacga
    15061 caagtacgcc aattaatttt ttcatgtact tcacctcttt ccttattatt aacgaaatag
    15121 aagatttcgt ctattgaaag ttgttggcaa cagctgaatt ttaaactttt cctcattttg
    15181 ttcttattgc ttcaaaagaa
//
LOCUS       DAZUMV010000059        14929 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000059 DAZUMV010000000
VERSION     DAZUMV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.059"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..815
                     /locus_tag="ACG8FK_001610"
     CDS             <1..815
                     /locus_tag="ACG8FK_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS5097219.1"
                     /translation="DKKIKKLASTQQLQSRKSKLLFLALVFEDQSYVIIDQSGHPVEY
                     SPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIPVLIEEKRSGKELANCWVGLPE
                     ERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWMLPNEIRKKNTADSLVLTKALR
                     SQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLLGSWQRATKRIREGKPVMIGIL
                     KVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYHKVIPASWSNGTVSLP"
     gene            895..1869
                     /locus_tag="ACG8FK_001611"
     CDS             895..1869
                     /locus_tag="ACG8FK_001611"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5097220.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            2049..3194
                     /locus_tag="ACG8FK_001612"
     CDS             2049..3194
                     /locus_tag="ACG8FK_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5097221.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            3331..3675
                     /locus_tag="ACG8FK_001613"
     CDS             3331..3675
                     /locus_tag="ACG8FK_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS5097222.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            3946..5232
                     /gene="efmA"
                     /locus_tag="ACG8FK_001614"
     CDS             3946..5232
                     /gene="efmA"
                     /locus_tag="ACG8FK_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS5097223.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(5370..5969)
                     /locus_tag="ACG8FK_001615"
     CDS             complement(5370..5969)
                     /locus_tag="ACG8FK_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5097224.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            6373..7494
                     /gene="mnmA"
                     /locus_tag="ACG8FK_001616"
     CDS             6373..7494
                     /gene="mnmA"
                     /locus_tag="ACG8FK_001616"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS5097225.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            7740..9161
                     /gene="arcD"
                     /locus_tag="ACG8FK_001617"
     CDS             7740..9161
                     /gene="arcD"
                     /locus_tag="ACG8FK_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS5097226.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            9175..10293
                     /locus_tag="ACG8FK_001618"
     CDS             9175..10293
                     /locus_tag="ACG8FK_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS5097227.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(10353..11645)
                     /locus_tag="ACG8FK_001619"
     CDS             complement(10353..11645)
                     /locus_tag="ACG8FK_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5097228.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            11795..12355
                     /locus_tag="ACG8FK_001620"
     CDS             11795..12355
                     /locus_tag="ACG8FK_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5097229.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            12484..12861
                     /locus_tag="ACG8FK_001621"
     CDS             12484..12861
                     /locus_tag="ACG8FK_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS5097230.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            12794..14866
                     /locus_tag="ACG8FK_001622"
     CDS             12794..14866
                     /locus_tag="ACG8FK_001622"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5097231.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         4881 a         2657 c         3238 g         4153 t
ORIGIN      
        1 tagataaaaa aattaaaaaa ttagccagca ctcagcagct gcagtcaaga aaaagtaaat
       61 tgcttttttt agctctggtt tttgaagatc aaagttatgt gattatcgat caatcaggac
      121 atccggtaga atattctcca gcaaaatata cttatcaaga gggtctttct tgtaaaaaat
      181 ggaagctgat caatgaagag ccaatcgagt tatctagatg gatcaacaga aaagaagata
      241 ttcccgtttt gatcgaagaa aaaaggtcag gaaaggaact cgcaaattgc tgggtgggat
      301 tgccagaaga acgttttttg aggtataaga aatgggctac cccctcaggc tatctttgtg
      361 gtacttacgc ggcagcagtt ctccttgcct attatcaaga ctatcgtaag gggtggatgc
      421 ttccaaatga gattcgcaag aaaaatacgg ctgattcgtt ggttttgacg aaggcattgc
      481 gtagccagat ccaaccacta ggacttccta cgattccttt tcaggttagt acagggattt
      541 ctagtttttt aaagaagaat ggcaatcatg agcgtgcacg agcaactctt ctaggttcat
      601 ggcagcgagc tacaaaacgt atacgtgaag gcaaacctgt gatgattggt attttgaaag
      661 tacttggcag tacatatggc aatcattggg tgacagcgta cgcttatttt gaaactgaaa
      721 caggagaacg ttattacaaa gtacacgaca actggggcga ttatcataaa gtcattcctg
      781 ccagttggag taacggaaca gtttcacttc cttaagaatt tgctgaaatt aacagaaaat
      841 cttggaaaaa tatgttaaac tatcgaagaa tagagaagtt ttggggagga ctacatgagt
      901 aataagtatc aggatgacac gttgaagatt tttagtttga atggcaaccg tccattagct
      961 gaaaaaatcg cgaaagtgtt cggaacagaa ttaggtaaaa gcgtcgtgaa gcaatttagt
     1021 gatggcgaga tttccatcaa tatcgaagaa agtattcgag gggatcatgt gtatatcgtt
     1081 caatcaacga atgcacctgt caatgattac tatatggaac tactgatcat gatcgatgca
     1141 atgaagcgag caagtgcgaa aacgatcaat gtcgtattgc cttactatgg gtatgctagg
     1201 caagaccgca cggcgaaacc tcatgaaccg atcactgcga aattgatcgc taacttgatc
     1261 gaagaagcag gggcaactcg agtactgact ttagatctgc acacggttca agtacaagga
     1321 ttctttgata ttcctgtaga taatttattt acgatgccgt tgtttgctca ttattaccgt
     1381 caattaggtc tgacgggaga tgacatcgta gttgtttcgc caaaaaacag cggtgttcag
     1441 cgtgcacgta gtctttcaga atatttgaac agcacattag ccatcgtaga tcatgcagat
     1501 gatgcttcaa ctgagggtgg ttatgtgatc ggtgatgttc aaggaaagac atgtatcatg
     1561 gtagatgata tcttgaacac aggagcaacg cttgcacgag cagcaaatgt attaaaagaa
     1621 aacggcgcaa aagaagtata tgcctgtgcg tcacatggat tattgtccga tcatgcgaaa
     1681 agtattttag acaatgcacc aatcaaagat atttgtatca ctgattctgt tttcacagaa
     1741 gaaaatcgtc atccagaaaa cttaacaatc gttacatgtt cagaattaat gggtgaagcg
     1801 gtcaaacgca tccatgaaaa tacaccgatg agtccgttat tccgtctgga agaaaaagaa
     1861 ttcgaataaa caaaaaaaag agggaaagaa gttgtgacag aagtgagcag cttcaaggag
     1921 ctgcgacaag acttttgtca cagctccttt tttctatgcc catgaaacgt cctctagaca
     1981 aaaatgagct cttctttggt ttgttcgcta ttttatgcta gaatgtgcct agcaataatt
     2041 tggaagggat gaatgaagtg aaaactattt atctggatca tgcggcaact acccccatgc
     2101 atccacaagt aattgaagcg atgacggcaa tcatgcaaga cacgtttgga aatccttcaa
     2161 gcatacatgg atttggtcgc cacgcgcatg aaaaattgga aacagcaaga caaatcattg
     2221 cagacagtct gcaagcaaaa cctcacgaga tcatctttaa cagtggggga acagaagggg
     2281 ataatacagc tatcttagaa acagcgttct cgcgtcaaaa agaaggcaga catctaatca
     2341 ctacagctat cgaacatcct gctgttctta ataccatgaa atatttagag acaaaggggt
     2401 ttgacgttac atatctgcct gtagatgaaa aagggaatct gtcgatcgaa gaggtcaaaa
     2461 aagcgttacg agaagatacg atcttggtct ctgtgatgta cgggaacaat gaaatcggaa
     2521 atttgatgcc aatcaaagaa atcggtgaac ttttagcgga tcatccagct tatttccata
     2581 cagatgcggt acaagcgtac ggaaaccaaa cgatccatcc agaagaatta ggaatcgatt
     2641 tattaagtat ctctgctcat aaaatcaatg ggccaaaagg cgtcggtttc ttatataaac
     2701 gagacggagt aaatatccct gcattgcttt taggcgggga gcaggaagaa aagcggcgtg
     2761 caggtacaga aaatttagca gggatctggg gtatggcaaa agcagttgaa ctgctgtcac
     2821 ctgaagaacg tgaagcacgt aacaacaaat ataaaggatt ccagcaattg attcttgata
     2881 cattagaagc aaacgaaatc ccttatcaag taaatggtga tttaacgaat aaactccctc
     2941 atgtgttgaa tcttcgctta attggcgaag caaatgattt gctactgatg aagcttgatc
     3001 ttaatggggt cgctatttct actggttcag cttgtacagc aggaaatatc gagccttcac
     3061 acgtattaga agcaatgtat ggagaagata caccgatact aaaagaatcc atccgtatca
     3121 gctttggttt gggaaatacc gaagacgaca tccagtattt ccttgatcaa ttagttcgtg
     3181 ctgtcaaaaa ataactagtg aagtcattct tatatgaaca gatgtctgag agaacgcctt
     3241 cattttttag ggaacttaac tgaaaaacct agtaaaaatc aggataaact tttataataa
     3301 agagaaacta attttatcga ggtgaaaatc atggcatttg aaacaacagc aacggtactg
     3361 ggcgcaccaa ttagctatcg tttgcatcct gatgcaaaaa gatatacatt gcgcgataat
     3421 ggatttacag aaacaaacgg aggcaattat caattgatcc gaccattaga tgccacgccg
     3481 caaagcaaag aaggattcaa attaaaaatc actgtatcaa aagatattca aacattgaaa
     3541 atgtcgatca ctacagcaaa tggattgaaa gcagtcaata ttttcaaaga tccaaaacag
     3601 aaaatgagcc aagataaatt ttattttctg atggatggga tgattagccg tgggttattc
     3661 gaaaaagtcg aatgatctgt ggatataaaa agacaactag ttttgaaacg gcctattgtt
     3721 agatgactaa ctttgtaagg acgacgcaaa ttagttgtct tttttatttt taaatttaac
     3781 acatgttgtt atttttaata ttttgctgat ttattgtttc taagtaaaga aatttaaatt
     3841 gaaacgattg ttatttaggg aaaaaggtat tttttgaaaa aagaatcccg tataatgatt
     3901 gttgtgcaaa cactatacaa aatgaaaaag aaaagaggga tgtttatgga aaatgaacag
     3961 tcggtcgttt taacgaattg gaagagaaat tatttgttct ttttatcggg acagtttttg
     4021 tcaggaatca ctagtatggt cgtccaatat gcgatcatct ggtatttgac aagagaaacc
     4081 ggttcggcga caattttgag ttttgctact ttactaggaa tgattcccat ggttttacta
     4141 agcccatttg taggaccgct agtggatcgc tgggataaaa aagctctttt gattgtcacg
     4201 gatattattg tagcgatttt cgcgcttatt ttagcagtag tcggaacgat ttcagaatcc
     4261 ttcccgattt ggctagtttt tgtgtctttg tttatgcgtt cggtcgcaca aacattccaa
     4321 atgccaacga tccagtcgat tatgccgaca atcgttccat cttctcacat aacaaggacg
     4381 aatggacagt taggaatggt ccaatcagcg aatttcatca ttgcgccagc tctaggtgcg
     4441 gctttgtttt cagtcgttcc tgtcaattac cttattttat tagatgtatt gggggcagtt
     4501 tttggtgtcg gcttattgat ttttgtgaag atcccaaaag tttctcctga aatattggaa
     4561 gttccgctta ctatttttaa agatgcaaaa tttgggctgc agcaattgat ggataataaa
     4621 gggttatggt acataacgat caatggtgca tttgtgatgc tgttgttcat gccagcaatc
     4681 agtctgtatc cattgatgac gctagattac tttggtggtt ccgttggaca ggcgggagca
     4741 gtggaagtag tctacgcagt cggaatgctc ttaggcggtg cactgatcag ttttatagga
     4801 acatggaaag accgaatgaa acctattatt atagcctaca tcattatggg cctgacgatc
     4861 ggtgcaagcg gattggttcc aaacgatagt caagggttct tgtactttct tatcttgaat
     4921 gcaggagcag gttgtgcaac gccatatttc aatacactgc tgatggcaat gatccagcag
     4981 agttatgaat cgaatgtttt aggtcgtgtc ttagggaact tcaactcttt gatgaacctg
     5041 gcaggtccaa ttggattatt atttgcagga ccattagctg atcgtttagg ggtagaaaaa
     5101 atgttcttgt tttccggaat cgggatttta ttatgcggaa tcgttttatt tttaacgtca
     5161 gctgcccgaa attatgataa agaattacag aaaaaactgg taaaagaaca ccatgagcaa
     5221 aaagacgaat agtatggaat ggagttgtga cagaagtctt gttacaactc catttctgat
     5281 cctaatgttc ctgtggcaat aagcctactc aaatgaaaaa tatgagtaat tatctttatt
     5341 tgtttaatac ttgatatagg gcagtttttt cacgacctat cgtacttcct ctttgcttca
     5401 tagatttctt tgatttgttg ctcagataac ggagtgccaa atggtagttt ttcctttaag
     5461 agttctcttt ccggtgcgtc tacgcctaca ttgacatcgt tctcgatagg tacgctgtaa
     5521 tgatggatat gtccatgaag attgcggata gtgtcgttag gcccgagcaa catcggataa
     5581 tgagtcaagt aatattgttg atggtcgaat ttaacgatgg cacctacatc ataaaaataa
     5641 aatttgggac ttccgtcagt aagcctagga tcattttttt ctaaataacg gaaaaaagcg
     5701 cgactgtcat ggttcccttt gatcaagtgg attgttcctt gcagttgagt gaccagttcc
     5761 aaaatttctg catttgcttt ttcatattgc ggatgcatag caaggtcgcc tagatgataa
     5821 acatggtcgg tttcttttat tactgcattc cagctagtga tcaactgttg gttcatttca
     5881 tccacatttg caaagagccg cggagcaaag tcgttttttc ctaataagcg ttcatggaaa
     5941 aagtgcatat cactgataaa gtaattcatg tatcttttgc ctcccttttt tcttagagta
     6001 aacaaaatca cttatctgag caactcataa catctaaaaa gaaaaggttg taaatgagac
     6061 gtgcagcttt aagcgataag aagcaatttt gaaaaatagt ttttcacaat ttataaaatt
     6121 attccttatt gccgaaaggc tggccctttt tatgagtatg ccaatttttt atatcgaatg
     6181 ctcaaaagat gagtgatttt ttggttgact atcatgtttg tgtactgaat aggtccagta
     6241 gaacagtatt tttacgactt gataattaag ggatgaaatt ttcagaaata tgacgtataa
     6301 tgtaaaatac tgaaaagttg cgaccttcaa ctcgtagatg tttcagaatc taattgaaat
     6361 gatggtgata tcatgacaga caacagcaaa acacgcgttg tcgtagggat gagcggcggt
     6421 gtcgactcat ctgtaacagc acttttgtta aaggaacaag gatacgacgt gatcggaata
     6481 ttcatgaaaa attgggatga cacggacgaa aacggtgtat gcacggccac ggaagattat
     6541 aaggatgtag cgaaagtagc tgctcaaatc gggattcctt attactctgt gaattttgaa
     6601 aaagaatatt gggatcgtgt atttgaatat ttcctagccg aataccgcgc aggacgtaca
     6661 cctaacccag acgtgatgtg caacaaagaa atcaaattca aagcattctt ggattatgca
     6721 atggatcttg gcgccgatta tgtagctact ggacattatg cgcaagtcac tcgcgatgaa
     6781 aacggcgtgg tccacatgct tcgtggagta gacaataata aagatcaaac ttacttcctt
     6841 agccaattat cacaagaaca gctttcaaaa acgatgttcc ctctaggcgg catggaaaaa
     6901 tcagaagtac gggcaattgc tgaacgcgca ggccttgcga ctgctaagaa aaaagattca
     6961 acgggaatct gctttattgg tgaaaagaac ttcaaacaat ttttgagcaa ctatctgcca
     7021 gcaaaaaaag gaaatatggt cactcttgat ggtgaagtca aaggacaaca tgctggcttg
     7081 atatattata cgattggtca acgtcaagga ctagggatcg gtggcggagg agattcgcaa
     7141 gaaccctggt ttgttgtagg aaaagatcta gcgacaaaca cattatatgt ggggcaaggg
     7201 ttccatcatc ctgcattgta tgccacaagc ttggatgcaa gtgaaatcca ctttacgacg
     7261 aatgaaccaa tgccaaaaga attcaagtgt acagcgaaat tccgttatcg tcagcaagac
     7321 gttcctgtga cagttcgctt attggatgat aatcgtgcag aagtcgtatt cgacgaacct
     7381 gtacgtgcaa tcactcctgg acaagcagtt gtattttatg atggaatgga atgtctgggt
     7441 ggtggtttga tcgaccacgc atatcaagaa acaaaagttt tacaatacgt ttaagcgata
     7501 ttaaatgaat caaaacagct atggctaaca aaagaaacac cttttctttt ggatagcata
     7561 gctgtttttt tgcaataaaa ttaattttta ttcattttat acttgaaata cttgtgtaaa
     7621 aagttcataa tatccaataa tatgaataaa gttgcttttt gttaagcaag agcagttgca
     7681 aaatagaagg gaatcttttg caagtgattt gttcaatttt ataagtgagg gtgatggaaa
     7741 tggaaaagaa atcgacgggt attagcaaat ggggactggt tgctctggtt gtcagttcgt
     7801 caatcggaag cggtgtgttt gggatcacaa gtgatctggc tagttctgca gctccagggc
     7861 cagcgatcct ttcatggata atcgttggga ttggtatttt agctttggta ttatcgctga
     7921 atcatttagg agaaaagcgt ccagatctag atggtggtat tttcggttac gcggaagctt
     7981 catttggtaa attaggtggc ttcatcagtg gttggggtta ctggttgtct gcgtggctgg
     8041 gaaatgttgc ttttgcaacg atgctgatga gcgccatggg agaatttttc ccgatattca
     8101 aaggtggaca aaatgttcct tctattttat ttgcaagtat ttttatttgg ggtctgactt
     8161 tattagtaaa caatggagtt gaaggagcga gcgtcatcaa tacaatcgtg acgatctgta
     8221 aattagtgcc attattccta tttttaatat ttgtatttat cgcatttaaa gtgaatatat
     8281 tcactgctga tttttggggt aacgtatctg ataatctagt gaatggaaca gggcagcaag
     8341 gaacgatctg gttgcagatt aaaggttgtt tgatggttat gatgtgggtc tttgtaggga
     8401 tcgaaggcgc aagtgtttta gccaaccgcg cgaagaaacg ttcagaagcc cagcaagcat
     8461 cgatcattgg tctgctttgt ttattgttta tctatatcct tgcttcgatc ttgccatacg
     8521 gcgtcttatc tcaagaagag ttagcaacta tttcacaacc tgctatggca aatattttaa
     8581 aaggaatcgt gggaaattgg ggagcagcgc tgatcaatat cggcttgatt atttcgatca
     8641 ttggttcttg gttgtcttgg acgatgctcc cagcagaaac aacgatgttg atggctcgag
     8701 acggtctgtt gccgaaaggt tggggaaaaa cgaataaaaa aaatgcccca acttattcgt
     8761 tagttgttac tgcaatcttg actaatctat tcttattgac tttcttagtt actgattatg
     8821 cctatcaatt tgcctattct ttatgtacag cggcaatttt gatttgttat ctacttgtcg
     8881 ggatctatca agtccagttt tctttaaaag aaggaatcaa aaaacaagta gtgatcggtg
     8941 ggattgctgt agtttttgaa ttgatgggca tcattttagc aggattttct tatgtccttt
     9001 tatgtagtat tgcctatctt cctggttttt gcttctactg gatcgcttgt cgtgaaacca
     9061 aacatcaaat tacgggaaaa gaaaaagtaa tgattgggct gatttgtgtg gccgcactgc
     9121 tttcaatcgg gttgcttgct gtaggctgga tcaagatata agggggaaag aaacatgaaa
     9181 atcgcaaatt tcggtgtaga agaatggcta aataaatggg aaaagaaagc ggtatatgat
     9241 attgcacaaa gctcgatcga agcattgaca ttagacgaac tcgttggctt agatgggact
     9301 agcgtaagta aatttttcga aaaacgcaaa aacgaaccat tggactatgg ctggatcgaa
     9361 ggatcagatg ttttcaaaca agaagtcgct tctctttatc aaaccgtcga tccggaaaac
     9421 atcttacaaa cgaatggagc gactggtgct aatcttttag ctttatacgc gttagtggaa
     9481 gcaggagacc atgttgtttc gatgcttcct acttatcagc aattatatga catccccaaa
     9541 tctttaggtg ccgctgttga ctttgttcat ttgaaggaag aagaagattg gcagtttgat
     9601 ttagaacagc tagaagcctt agtcaaacct gaaacaaaaa tcatctgttt gaatagtgca
     9661 aacaatccta caggcacatt gctagatcgg aaaactttag aaaaaatcgc agaaattgct
     9721 cgtacagtag atgcatatgt attgatagat gaagtatatg cgcctttaac ggataaaggt
     9781 gaattcttgt cgatcgtaga tgtctatgaa aaaggtatag caaccaattc cctatccaaa
     9841 acctactcca ttccaggtat tcgtattggc tggacagcta ctggacctga gttggcagaa
     9901 gtttttcgaa agtatcgaga ctacacgatg atttgcggcg gagtcctgtc agatgatctt
     9961 gctgtacatg cattgaagaa taaagaaaaa atattggcgc gaaacaaaaa gatcattaca
    10021 gagaaccttg tgattttaaa acaatgggta gccaatgaac cgaaagttga acttgtcgct
    10081 cccaattacg tctctacttc tttcattaaa ttagccatcg aagaagatga ccagacattt
    10141 tgtatcaatt tattagaaga aacaggagta ctattagtac caggaagtgc ctttgattta
    10201 ccgaagcatg cccgactagg ttactgttgt aaaaaagaaa ccttggaaaa gggattattc
    10261 ctcttatctg agttcttgaa aaaacaggcc taaacaaaaa cactgcaagg acagtctgtg
    10321 acaactgttc aagcagtgtt tttcatttac gcttattgtc tatgagaagg agagtgccct
    10381 gaagctatgg agactcctga agctttttct tctgcagttt cttgctgatg gatctcttgt
    10441 gtattgtgtc gtacaagcaa gaagttgacc aaaacaagaa gagaagtcga aagaaagacg
    10501 ccacgataac caaatgcact ggaaacactg gagccgatca taggccctac tacgttacca
    10561 gttgcttgga aagattggtt ataactgaag atccgtccag cagcttgatg aggcgaatat
    10621 tttgtgatca atgcttggac agcagggagc agacaagcat cagagatacc aatcaaaaaa
    10681 cgtaaggcag ctaattgcca aacattcgtg acgaatgcca ttgggatata aaccagaatc
    10741 gcaaaaccca aaccaatcgc aagtattctt tcactcccaa tcttgtcacc taagcgacca
    10801 aatcttggag cggctatcaa agtggcaatc ccaggtatcg aagcaatgat tccgctgatc
    10861 aacgtaacat tcccatgtcc atgcagcaat tggcgaatgt acagactgat gattggacta
    10921 atggaattgt tggatgcttg gatgatcatc gttgtgacga acataccgat gacgacgtgt
    10981 ggatatttca agtctttgaa aatctgtttg gcagaaacca tgttttcttt tttcactggg
    11041 acaaattttt catggacgaa aaacagactc aacaagaaga cgaaaaataa gatgatccct
    11101 gtaatgaaaa aggtagggcg atagccaaac gctgaagcag agatcccgcc aagaagcggt
    11161 ccaagtaatg ttcctgtaac agaaccagtt gctaaggttc caagaacttg accacttttt
    11221 tcttttggcg tcccagttgc gacaagggca gtggcattac tgatataacc agaaaaaatc
    11281 ccttgaagca gacgcaatgc gactaattga tacacactag tcacgcaacc catcaaagaa
    11341 atgacgattg ccattcctaa tgaagcacgc aacagcatca attttcttcc tttttggtcg
    11401 gctaattttc cccaccaagg agagataagc gtagtcacca agaaagtaga tgaaaaagtc
    11461 aatccgctcc aaaagttcaa ttgtgacgta ctataattac ctaacgtatc tatgtaaagt
    11521 gacataaaag gcatgactaa actaaatccg ataccagcca taaatgttcc aaaccataag
    11581 acaaacaagt tttgttccca agtttctctt ttcctaaata atttttcctt tattcttgca
    11641 gccaattaga ctccttcttt ctgaaaattt tcatctacag ctctttatta taaactacaa
    11701 tatgtagttt atcaatgaaa gtctcttttt tctctttctt tttcgtggta aactgaaaga
    11761 gtgaaggatt tattttatga gaggaaacat ggaaatgaaa aaaagaaatc tgtctcaaga
    11821 gaaaatcatc gactgctttc gagaactagc agaagagatg gatgttcagc aaataacctt
    11881 tcagcattta gccaaacgct tggatatcaa ggctccctcg ctgtataacc atttcaaaaa
    11941 tattcgtgat gtcaaaacag cgctaacagc gaaacttctg caggaactta atgaccagct
    12001 gcgacgggct cttgtgggaa aaagtggtca ggaagcactg cagatctatg cagaaactta
    12061 tcgatccttt gctttttcaa atcaagcagt gtatgagtta ctgatcagtg taccgcatac
    12121 caatgaagct gttttattag atggaatcca tgaaacgaat cagatcattt tgcagatatt
    12181 tgaggctttt caattaacga aagaagaaaa agttcatcga agcagggaac ttcgtagtat
    12241 gatccacggt tatctttcct tacgtttttt aggatacttt acaaaagaac caacaatcga
    12301 tccagaagac agctattttt ggatgatcaa taactttatc gctactttgc cttaaacaat
    12361 cagattcatt tgtaacaatt ttaagatgtt cattaaatga cccttttata agagaaaatt
    12421 cttgcaactt tcttgaaaag tgactaagat gagtctagaa aagctgaaag aaggagcacg
    12481 agtatgtatc aagtaattac catgttcggg gacaatgagc cttggtggtt ttttgaagac
    12541 tgggaagaag atattgaaga agaagaaaca ttcgagtctt tcgaagaagc acgacaagcg
    12601 tacgaaaaac aatggaacga aatccatcaa aattatgaat atatcaatgc aaaaagtaac
    12661 tttctcagcg ccttttggaa tgacggagat gagcgctggt gtgaagagtg tgacgatgat
    12721 ttacagcaat acaagggact tgcgttgttg aaaaatcatc agcccatcac agtagaaagc
    12781 agaaaggaat tttatgaaac aactaattct agcggaaaaa ccaagcgttg cgaaagacct
    12841 aagcaaggtg cttggtgcta atcaaaagca caaaaattat tatgaaggac ccaaggtgat
    12901 cgttacttgg gcccttggtc atttattagg attgaaaatg ccggaagatc tgaataagga
    12961 atggcagagc tggcaaatgg acacgttgcc aatgatccct aaaaaactag gaatcaaacc
    13021 acttcctaaa acaggacatc aattaaaagc aatcaagcag ctggctcagc gaaaagatgt
    13081 ctcagaagca gtcattgcga cagatgccgg aagagaaggg gaacttgttg ctcgctggat
    13141 cttagagtat gtgcatttca ataaaccagt gaagcggtta tggatctctt ctcaaacaga
    13201 taaagcaatc aaaactggat tcaagcaact aagacctgcg aaagaatatg atgcattgta
    13261 tgaatctgct cttgcccggg caaaagcgga ctggctggta ggattgaatg tgaccagagc
    13321 cttaactgtg aagtacgcgg ataatttatc agcaggccgt gtgcagacac cgacactagc
    13381 attagtgaga gaccaagaaa agaaaatcga gcagttccgt ccacaaacgt atttcacgat
    13441 cacgttgcaa gctggaaaag aacaagccaa aatgcttcaa aaaaatcaat ttgctttaaa
    13501 aacacaagaa gaagcacaaa aacttgttca attgatcagt acaagcaaag gaactgtcac
    13561 agaagtccaa gaaaaattaa aaacggaatc tgcgccattg ccttatgatt taacagagat
    13621 ccaaagagaa gccaatcaac gctatggcta ttctgcaaag aaaacattaa gtcttgtaca
    13681 aagtttgtat gagacccata agatcgttac ttatccgcgt acagacagca aatatctgac
    13741 aaatgacatg aaaagtacga tgaaagaacg attgcaagca gtcagcgact ttgctcctga
    13801 ggtcaagcag tatttgaaaa acggagccat cgtccaacag acaaaagtat tccaagacag
    13861 taaagtgacc gatcaccatg cacttttgcc aactgaaaac agagcacgtt atgaaaaatt
    13921 aagtaatgaa gagcgaaaaa tctatcaaat gattgtctct cgttttttag gactgtttgc
    13981 tcaaccccat aaagtcagcc aaacaaaagt tacggttgaa tttggtaaag aacgattcat
    14041 ttttcggcaa aatcgtgtcg tccaagcagg atggaaaggc gaaactgaaa ctgagacgga
    14101 aacagtcaaa tgggaaaaag gaatgagaat caatcccgat ttcgccatca aaaaagaact
    14161 atctgcaccg cctaaaccat tgtcagaagc aagcttgctt ggtcaaatgg aaaaatacag
    14221 tctgggaacc ccagctacgc gtgcagagat tatcgagaaa ctaatcaaat ctgaactgat
    14281 ggaacgtacc cctcaaggac tgcaagtatc tcctaaaggt aagcaattat tagtattggt
    14341 aaatccctct ttagtaacac cagatttgac agaaagctgg gaaaaagacc ttgaagctat
    14401 tgctgcagga aagaaacaag caggaacctt cttgaatgga atcgaaaaag aaacaaaacg
    14461 cttggtgaat gagatcaaat caagcaaaca agaatatcaa gatttctcga ttacccaaaa
    14521 gaaatgccca gaatgtgggg ccaatctgcg cgagaaaaac acacgtgacg gcaaaatcta
    14581 tgtctgcacc aattcagatt gcaactaccg cagaagaaaa gatccaaaag tctctaatca
    14641 tcgatgcccg caatgtcacc ggaaaatgga aatcatcgaa ggtaaaaacg gtgcttattt
    14701 ccgttgtaaa tacgatggaa cgacagaaaa attgcttgat aaaaaagagc agaagaagaa
    14761 aatgaccaaa catgaagaac gacgcttgct gaaaaaatat gcacaagcag aagaaccaga
    14821 agaaagccca ttagcagcag cactaaaagc agcaatgaag aagtaataag atatgaaaga
    14881 ggttgtgata gaagtggtca gcttcaagaa ataagaagga atttacgaa
//
LOCUS       DAZUMV010000060        14772 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000060 DAZUMV010000000
VERSION     DAZUMV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.060"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            364..684
                     /locus_tag="ACG8FK_001623"
     CDS             364..684
                     /locus_tag="ACG8FK_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS5097232.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            845..1750
                     /locus_tag="ACG8FK_001624"
     CDS             845..1750
                     /locus_tag="ACG8FK_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097233.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1747..2520
                     /locus_tag="ACG8FK_001625"
     CDS             1747..2520
                     /locus_tag="ACG8FK_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5097234.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(2697..4121)
                     /locus_tag="ACG8FK_001626"
     CDS             complement(2697..4121)
                     /locus_tag="ACG8FK_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS5097235.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4332..4778
                     /gene="nrdI"
                     /locus_tag="ACG8FK_001627"
     CDS             4332..4778
                     /gene="nrdI"
                     /locus_tag="ACG8FK_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5097236.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(4848..6830)
                     /locus_tag="ACG8FK_001628"
     CDS             complement(4848..6830)
                     /locus_tag="ACG8FK_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5097237.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLEGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6844..7740)
                     /gene="murQ"
                     /locus_tag="ACG8FK_001629"
     CDS             complement(6844..7740)
                     /gene="murQ"
                     /locus_tag="ACG8FK_001629"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5097238.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(7743..8804)
                     /locus_tag="ACG8FK_001630"
     CDS             complement(7743..8804)
                     /locus_tag="ACG8FK_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5097239.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(9016..9543)
                     /locus_tag="ACG8FK_001631"
     CDS             complement(9016..9543)
                     /locus_tag="ACG8FK_001631"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5097240.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(9610..11112)
                     /gene="eat(A)"
                     /locus_tag="ACG8FK_001632"
     CDS             complement(9610..11112)
                     /gene="eat(A)"
                     /locus_tag="ACG8FK_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5097241.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(11370..12491)
                     /locus_tag="ACG8FK_001633"
     CDS             complement(11370..12491)
                     /locus_tag="ACG8FK_001633"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5097242.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            12617..13558
                     /locus_tag="ACG8FK_001634"
     CDS             12617..13558
                     /locus_tag="ACG8FK_001634"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5097243.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13701..14597)
                     /locus_tag="ACG8FK_001635"
     CDS             complement(13701..14597)
                     /locus_tag="ACG8FK_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5097244.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
BASE COUNT         4295 a         3129 c         2634 g         4714 t
ORIGIN      
        1 tttttttatt atataattca ccttacttat tgtaagataa agcttttttt aatttattgc
       61 ctgctaatat ataaaaataa ggtctagtta ctgttttttt aaatgattaa taaataacaa
      121 ctcctttcta tgaaattgca aagcattcat cttcttttct gctaacgata tatccgtcaa
      181 tgcttgttac actttactaa aaagtatgag attgccgata tctggatagg tgtgctatac
      241 tacagaaaaa gatatacgat ctgccaccgg gtagtaaaaa agaggatgat cattccatta
      301 ggtctttgaa gggatgattc tcctcttttt gtgtattttt tatattgaag gaggaacgta
      361 tgtatggcat ggatggaatt aatcatagca ggaattttgg aagtattctg gtcaacgatg
      421 atgaaatgga gtgatggttt ttctaaaatc aattattctt tttacacagt gattggaatg
      481 attgctagtt tttacttttt gtcaaaggca atcaaaagtt tgcctatgag tctctcttat
      541 cctatttgga cagggattgg cgcagttggt tcggtcatta ttggtgtggt cttgtttcat
      601 gataagctga atctttccac ttggttcttt gtcgggttgc tgctcatcag catcattggc
      661 atcaaaatca ctagcggtca ctagatactt agacaagcag acaaggactg atcagaaaaa
      721 gcggggcttt ttatttgata ttcctctttc tatgaaaaaa caagaaaata agactaatga
      781 cctagcgact tctctactca tcaggtgtat gcttgtacta tctatagaaa aggagtgaat
      841 atttttgaaa agtctttcgg tccaagaatt atccaagaac tttggtcaca aacaagccct
      901 cagcgatgtc agttttagct tatctcctgg caaaatcgtt ggattagtag gtcctaatgg
      961 cgctggaaaa acaacgatca tgaaagctat tttaggactg atccattact cagaaggaac
     1021 gatccgtatt ggtgaaaatg agatttctcc ctctagtcat aaaggattag aacaagtagg
     1081 agcattgatc gaatatccgg gtatctatcc attcttaaca ggttatgatc atctgaagtt
     1141 attctctgaa acaaacgatg tatctgctat cgacactatc gtgaaacagc tcaagatgga
     1201 aaaatacatt cacaaaaaag cgaaatccta ctcgctggga atgaaacaaa aattgggaat
     1261 tgctcttgcc ttactgaacc atccgtctat cgttatttta gatgagccga tgaacggttt
     1321 ggatccacaa gcaacaagag atgtgcgaaa aatcattacc gacttggcta ctcagggcac
     1381 ctctttcttg atctccagtc acttactcag cgagcttgaa aaaatggtcg atgagctgat
     1441 cctgatcaat caaggaaaaa tcatcaaaca atgtacaatg gctgaattga accaaacagc
     1501 gcaagatttc atcgttatct ctacgacaaa tgatcaagct gctcgaacga tcttgacaga
     1561 cgctggatat cccttggaag atcctgataa aatcaagctt ctaaaaaaaa acgaagagtt
     1621 attaggaact gttatccagt tattgtctgc agaaaatgtc catgtgacag atgtccagca
     1681 tctgacgaat gatttggaat cttccctatt ggaaattctg gattcagtaa agggggaaga
     1741 agaaaaatga cgttgcttat aaaacaagaa ttatttaaat tgatcaagaa aaagtcaact
     1801 gctgttttat ctgttctatt agttgctttg ctcataggaa cagcacttct agccaaaaaa
     1861 tacacgacga tcattgatcc agttgaaatg actgctcagc ttttcagtgc cacatcttgg
     1921 attgtcttta tcatgattgc tgctgcaagt acgatcattt caatggaatc acaatatggt
     1981 acattgaaaa atctccttta tcgaaagtat tctcgaggag aaatattagt aagtaaatgg
     2041 atcacattag ttatttattc tatttattta taccttttag ctattattgt caccgtgctg
     2101 atgaaactga tccttttccc ttcgatttcc tttacggaga agatttctac tggtcagaca
     2161 ctgatccaaa gcttattcac ttatactttg ggtagttata tcggtttatg gctaatttta
     2221 agtttagtgt tgatgattgc ttgttttatc aatagttccg gtgcctccat ctcagcaggg
     2281 atcgtctttt attttgcttc atcaatcata tctggtatcc tcatcgcact cattcaaaaa
     2341 tgggagtgga tcaaatggaa tccaatcagt atgctgaatc tgcaaaacca aattggcaac
     2401 gaagagatta tgaagcaatt gactcatctt tctactaatc aaatgctgtt tggcaattta
     2461 gcttacattg ctttgttctt agcattggga tacctcgtat tcaagcgaaa aaatgtatga
     2521 tggttcatta acaaagaagt tgtgacagaa gtggccagtt tcaagaaatt tcggcttatt
     2581 tttagaagaa gctacttctt gaacaccatt tattcaaaaa aagaagcgat gacacgaatg
     2641 ttgtcaccgc ttcttttttg aataaaccac aggcttcaca gcctctcttt atacttctat
     2701 acgtgaagca tcccagtggc aaataaaacc attgcgatta ctccgaccat cgtcacaccg
     2761 ataagtaaaa tcttatctaa gtggatatgt tgtacaggtt cgttttgttc tttacgtgct
     2821 ttgaggtaaa aataaatacc tggtaagaaa agaacaagaa caagcatcac ttcattaaag
     2881 gctacaacta gcatggccca tacttgaaaa gcacttgcta aaaaccctac taaaaattgg
     2941 acccactctt tccgctgata agaaagcttc atttggtaca gccctaagaa aagccacgtg
     3001 aagaaaatcg ctgctgtacc aagtgtataa gcaaagttat atgcactgtc agtgaataat
     3061 aatgaaagta aaaagacatt cgtacataac gtcgtcaata cgatagcata agttggtgcc
     3121 ccatttttgt tgagttttcc ccatgcttta ggcaaaagtc cgtcctcagc catattcccc
     3181 attgtttcaa tcggcaagat tgtccatgag agccaaaccc caactaaaga aatgatcaaa
     3241 ccgccgttga tcacgaccgc cccccattta ccaaccatca tttcaaatac atatcccata
     3301 gcaggttgcg ctttgatcgt ttgcagctga ttcatcgtca ttgtgccata tggaagcatg
     3361 gaaaccaaga cgtaaataat gatcaaaccg aatattccta gaatcgttgc tttcccaaca
     3421 tccgattgtt tttttgctcg tgaacctacg acagaagctc cttcaatacc aacgaatacc
     3481 cagagcatca ccatgatgca actcaatact tgactagaaa acgggatcgg ctgcccattc
     3541 gcattcaata tgatatcgct agaaaagtta ttaatgaatt gttggatcga aaaggtgctg
     3601 atagctacta caataaataa aaataatggc actaatttac aaaatgtgac gatagcattg
     3661 acaaagctag cattttcaac tccacgattc actagccatg catatcccca taagaagatc
     3721 gtactcatca cgatcgatgc cacattttga ccaccttcga atacggggaa gaaataacct
     3781 aatgcactca tcaacaatgt tgcgaacgcc acgtttccta accagcatgt gagccagtaa
     3841 ccccaaccgc tgataaaacc agcgaaacgg ccaaaacctt cttctgcgta actaaagatc
     3901 ccgcctttta aatctggacg ttttttattg atattattga tcgataagga gagcatcaac
     3961 attgctgttc ctacgagtac ccagctgaag atagctgggc caccagccga tccggagatg
     4021 tccgtcataa taccaaatac gccacctcca atcgctgaac caatcactaa ggcagcaagt
     4081 ggaatcaaac caattccttt tttctgttgc tctgtatcca tgtaagcatc ctccaaagta
     4141 aaaccgcttt tatcaaaaag tataacaaac cttttttatt tttacttaat taataaaaac
     4201 tttaatagaa atataattct accgactttt ttctcattat acaagcaaag cgcctagtcc
     4261 ctttccaaat tacaccacca aaaaaactta aaaagatgct atactaaagg gaaagaattg
     4321 ggggatgatc catgaacatt ttatatatat cgatttcagg aaatacgcgt gcttttgcca
     4381 aacatctagc agaatatgct gaaaaaatgc atgcagaaga tcctgtcaat cctgaagtta
     4441 ctttaaaaga aatccatgaa aatagtgatt ttacgaaaga aacagaacca ttttttactt
     4501 tcgttcctac ttatttagac ggcgggaatg ggttagacaa cggtgacaca gaaatcttga
     4561 ccgaaacgat gcgcgaatat ctggaatacg aagacaatca taaactttgc cttggtgtag
     4621 tcggtagcgg taataaaaac ttcaacaatc aatattgtct gacagccaaa caatacgcac
     4681 aaagattcgg ctttcctttc ttggcagatt atgaattacg gggaacacca tctgatgtga
     4741 cacgcgttta tcaaattttg gcagaaaaaa ataaataaca caaaagaaga agctgaaaaa
     4801 taaatttcgg cttcttcttt ttatgttctc tattgtttct attcttatta agctttgaca
     4861 tccatcacag gtgcttttgc tttcgcttta cctgtttttt ccaagacaaa gttttttaca
     4921 cgatccatat tggttaccaa gacgatcatg gaggtttctt tgtctgctgc tttgattgct
     4981 tctaagtcag cttctgctac aagcgtatca ggtgtcactt gctgtccttc tgttactttc
     5041 acatcgaatg gcgtaccgcc taagtcaact gtgttgatgc ccatgtgaag caagatttct
     5101 aagccatttg gtgtcgtaat gccgatagca tgttttgttg ggaaaacaga acttattact
     5161 ccagtaaccg gggaatagat atgtccgttt tctggttcta caaagtaacc ttcacccatc
     5221 attttttgtg caaaaactgg gtcacttgct ttatctaaag aaacaactgt tccgtcagca
     5281 actgatgata agatagtcac actttcagct gaagcttgag gtactgcttt ttcaggtact
     5341 tcatttactg tggctgtttc ggtcgctagt tcttcttttt ctaaacggtc tttaggaata
     5401 ccgaagaaga atgtagcaac gaagccacca gcgtaagcag ccaataaacc aaggatatag
     5461 cctaaccatt taccatcaga gatcaatgga atcaaagcgg ctcctgaagg tccaatagcg
     5521 attgctccga catttccgat cattccgatc actgccccac cgatacctcc accgatacaa
     5581 gcagtaataa atggacgtcc tagaggtaat gttactccgt aaatcaatgg ttcgccgatt
     5641 cctaagattc cgactggtaa agcccctttg atcatttctg ttaattcttt gtcttttttg
     5701 cagcggatcc ataatgccaa tgctgcaccc acttgtcctg ctccggccat cgcaaggatt
     5761 ggaagcaata aggtacgacc agtttcatca atcatttgta catggattgg cgtcaagatc
     5821 tgatgtagtc cgaacatgac cattggcaag aataatgtac ctagaacgaa gccggcaaat
     5881 gctcctccaa caccaagtac ccagttgatc ccaccgacca tgccgtcaga aatgaaccct
     5941 gctaaaggca tgatgaagaa aatttcgatc agaccgatgg caatcaggga aagagttggc
     6001 gtaacgatga tatctaccgc atcaggtact actttatgca gttttttctc aacgattgac
     6061 aataaccaaa ctgcgaagat aacacctaag atcccgcctt gtccagttgc aagagcttca
     6121 ccggtaaaca agtttttcag tggtgcttct ggattcatcc ctgtcagcat cacaacagcc
     6181 ccgataactc caccaagcgt tggcgttgca ccaaaaactt gtgcagcatt gatacctgta
     6241 tagataacta gatagctaaa catcccgttt ttcaagatgt tcatcacatc gatatattgt
     6301 tgccatgttc ctgcgtctag tgttccagct gtgactaagt ttgctaaaac tgcggcaatc
     6361 cctgccacga tcccagtgcc gacaaatgca gggatcattg gtacaaagat attagagatg
     6421 tcttttaata ccgatttgat tttagagggt ttttgtttcg atttttgtgc cgctttcatt
     6481 tcttgtgccc gttcgtttac acggtctttt ccagatgcag aagaacttgc tgtagttgca
     6541 ctagcagaag gaagtttttc tcctaatttc acgccttcta agtcaaccat ttcctgcgct
     6601 actttgttca ctttcccagg tccgatgatg acttggagct gttcatcctc tactacgcca
     6661 agtactcctg gaatcgcttt taattccgag acatttacaa gatcatcatt gatgaccttc
     6721 atacgtacac gagtcatgca gtggacaatg ctttcaacat tgcccatacc gcctgctcct
     6781 tgataaattt gttttgccag ttcttccaaa gtcaattcgt tttttgccat ggtcttttcc
     6841 ccctcataag gttcctttta caaaaccgtt tgcttcagct aattttgctt gagctgcctc
     6901 tttatcacta tttgtcaaaa tcatgacgat agccagtttg acattttgat ctgcttcctc
     6961 aaagtacttt tcagctgttt cgtagtcagc ttctgtcgct tgcatgatga ttcgttttga
     7021 gcgttcaatt agtttttcat ttgttggttt gacgtcgacc atcaagttat tgaatacttt
     7081 accgataccg atcatactga tagtagaaat catattcaaa atcagttttt gtgctgttcc
     7141 tgacttcaat cgagtagagc ctgtcaaaaa ttctggtcct gcatcgactt cgattgggaa
     7201 atccgcatgc tcgctgattt ctgcttgttt attgcaagaa atcgttcctg taacagcacc
     7261 aatcgatttt gcgtaatcta atccaccaat cacatatggt gtacgaccgc tagctgcgat
     7321 accaataact atatcatttt ttgttaaatt cagcttgatt agatcttctt gccctaattc
     7381 ataagaatct tcggctcctt cgacggctac agtcattgct tcttctccac cagcaattag
     7441 gcctatgacc atttccggtt ctacaccgaa tgtcggtaca cattcagcag catctaatac
     7501 accaagtcta ccactcgttc cagctcccat ataaatcaag cgaccacctt ttttaaagga
     7561 ttcaatcgtg ttggcgatca ctggctcgat acttggcagt tctttttcaa cagcagcggc
     7621 cactttctga tcttctttgt tcatgagttg gactgcttga gaaatactca tttcatctaa
     7681 tccaaaagtt tcttggtttc tgcgttccgt tgttaggttc tctaaattga ttttgctcat
     7741 ttttatcttc cttcttttct tataaattga tagttcatac cagctttgat ttggttgatc
     7801 agcggtagtt cttctttgat gactttggct actacattca ctttttcatc cgctggcaaa
     7861 tcatatttag ttaattggat ctcacccata tagcgcaaat acttttcgtt atctaaagta
     7921 atagatcctt tcatacgctt agctgtattt cttactggga ttggcggaat ctctcgaaaa
     7981 cgcgcatctg cgcttcgtat gacatctctt gcatcatctt gccgattcgt atgttttcct
     8041 aatatatatt cgtaaaaaac ttcatctaca ggttccgtat ggagcaagac tgtctgttct
     8101 ttttggaaag aagcaaattg ctcctgaacg tgttctgaca gacctgcatc tccgatataa
     8161 acaatatccg tgttacttgc taatagatca agagcacctg ccagaggatg gatccctcga
     8221 tgttcttcca aagttggtaa tccttgatac agtggacctc tcaactctgt atctcctggt
     8281 acaaagcctt gtacaaggaa tccttgagtt ttgagccatt gatttttttc ctgataccat
     8341 tctttgtcca accctgtttc aagacgtgga taataattat gccaagcttc aatctgtgag
     8401 aaatcagccg ctgcttcttt caactcatcg atatcattag gcgtgatcgt gctggcattc
     8461 aaactgattt tcaattgatg tgaccagtcg gcgatctgct gattagaaat atgataatcc
     8521 attcttaacc ctgttacacc aattgctttc aagggtgtta gatcttctaa tgaaaagcca
     8581 gctcgtttca atgcctctcc tgaaatatcg accatcaact ctagtttatt ctttttcgct
     8641 tcgcttccta gttcgatcaa tcgattttga taagccgaaa catcatcttc cggtatatgc
     8701 atggaagtga aaataccgac aaagccattg gatgccattt ggcggatata ctgtttcttc
     8761 tcttctgaca aagaatcatt cataaatatt gaaaagccaa acatattatc cctctttttc
     8821 tgtatccgtc ttcatttcta tgactagagt atatatctaa agaaacaaaa tttcaatact
     8881 tttgttaaaa taattaaatt ttatttctaa aaattggatt tataatgcca aaagaattga
     8941 cacagccaca aaaaaggcac tgctaagcaa aagcagtgcc ttgaaacatt ttatttaaat
     9001 aacacaaaag tctaattact cataagaaat taattctgta ttgtctacca tgaatgtaaa
     9061 gaacccttca ttaggcattg gatgaaaata agcattgatc actcttatca aggcattatg
     9121 acagactaac aagtagacat tttcttcgtc ttccatgcat tctttcaata aaggaacgat
     9181 ccgtgcgtac acatctaaga ccgactctcc atttgggaaa cgtacagcaa acgctaaacg
     9241 cgctttttga aagttttcat ctttgcttgg cagtccgtca taatccccaa aatccatttc
     9301 taccaatcgc tcatcagcgg tcatcggcaa attgagacga tcaactactg cttgtgctgt
     9361 atctcttgct cgttgaagag gggaatggat gattttcgtg attggaacag gcaaatctac
     9421 taccttttct gctagtgcct gtgcctgcat ctgtcctttt tcagtcagcg gcacatctgc
     9481 tcgcccgcaa actaatcctt gtgcgttcca agttgtttct ccgtgtctcg ttacataaag
     9541 catgtgattc tctccttcgt tgacaaagtt ctccttaacc ataccttgtt gtctttgaga
     9601 ttacaaggtt tagattttct caagagaaat aatctcagta gatactttat ctagaaaggt
     9661 ttgatcatgt tccactagta acatagcagg tttcacgttc aaaatcagtt gttctaattg
     9721 ttcttgattg aagacatcca aataattcaa tggttcatcc catatataga gctcagctgg
     9781 ttgggataaa gatttagcca attccacttt tttccgttgt cccatactca tctgttcgat
     9841 tttattatga aaaacatctc tttccatccc aagctttcgg agattattca aaaatgcttg
     9901 atagtctact cggtttttct tcgcgaattc cgccaatgtc cctcgattgt cttcataatt
     9961 ttggcgtacg taactaatgg tcaaatgttt gggctgggat ttttctccta caaccttgcc
    10021 gttgaatgtc cccagaagat aatggataat ggatgacttt cccgcgccgt taggacctga
    10081 aattgccatc cgctgatgag gttcgatcgt aaaatgaact ggctcgaata acagattttc
    10141 ataccctaat tgaagatttt ctacgcttaa cagccgttta tgatgagaca cttggctatt
    10201 catcgtcaaa gcatcgatat actcgatatc ttttaataat ttttctttct cactgatctg
    10261 ggtttccatc cgattcacga ttgctttaga tcgtttcatc gtccgtgcag catcagcgcc
    10321 tactgctcct ttattcactc gcctagattc cgtatcgatg aatccgactt ttttctttgt
    10381 tttgtcccct tctcgggaac gagaccattc tgctttttca gctgctgttt tctttagcct
    10441 gctgacttct tttttcaatt tttcattttt agctatttca aattcatcac gaagtttttt
    10501 ctgttcttca tagatagaga aattcccttg ataaagttcc agttggctct tttcgattgc
    10561 taaaacatgg tccactactt catcgataaa tccccgatca tgactcacca cgatgaatcc
    10621 ttgttttttc tttttcaagt aagccgctac ttgttttctg ccagagatat ccaaatgatt
    10681 cgttggttca tcgattaacg ggaaatgagt gtcatccaca aataagagtg ccagtaggac
    10741 ttttgtcttc tctcctcctg atagtgtgct gaattctctc cataagattt caggatctgt
    10801 ttgcatcagt tggagctctc tttcaatttc ccatatctca aaatccataa tctcatttaa
    10861 cacataatag gtcaaacgct ctttatcttt tgtttgctgc gggaaataga caaacgcttg
    10921 ctgatggatc acttgccctt gataagggag tttgttttgc aggatattca gtaaagttgt
    10981 ctttcctcga ccgtttcgtc cgataagtcc tagtttccat tgtgtgtcaa agtttagatt
    11041 ggcttgttca aataataatg tgccttggct gtcatagccg aatgtcagat tttttatttc
    11101 gattttagac attattccat cacctcgtaa aaaaagacca gtcctatttt cagaactggc
    11161 taaagaggat gcaagggttg gggcacacaa aaagacccat acatcgaaag aaccaagcca
    11221 atcccgaaaa tctgcacact gctcccttgt ctccacaagg tacagtctga atgcagcttt
    11281 catcaatcga cttaattttt caatgcacgg atccctcact ttgtcctata tttgaaatta
    11341 cgatagcata aagaaattgt ttttgtcaac tacttcggga gaatgttgaa aaatctgctg
    11401 agtgtctgtc ctaagatttc tgttttttct aatgtttgcc tgtgttccat tgcttcctct
    11461 aaatcgattg gtcctaattg aatgcttaag acaatgggga gaaaatcaaa gagtccttcc
    11521 agctctcggt ctctacttcc gcaaatagcg attattggtt ttcgatattt ttttgcaagc
    11581 actgctacgc catatggaac ttttccgtgg attgtttgct gatcaatccg cccttctcct
    11641 gtaatgatga catccgcttg ttcgatctgt ttttctaatc cagtgatttg gctgaccaac
    11701 tcaaaaccag agaccatctt cgctcctaac aaaaaaaggg ctccgccaat accgcctgct
    11761 gctccgcttc ctgggatgtc atctaaatcc acctcccaaa catcttttat ctgctgagca
    11821 atcttttcag cttgtttatc caagaaagct aacgtcgtat ccgttcctcc tttttgtctt
    11881 ccaaattgcc aagcagctcc ttgttgacct gtatatgggt tggtcacgtc tgcagcgatg
    11941 atcactttta ctttagaaac aagaacgttc cttcgatcca aatgaagttt tctagtagat
    12001 aacaaaggat ttccttctgt gtacccttct aaagaagcac ccaaagattg gagtaatcct
    12061 agaccaccat cgctacatcc gcttcctcct aaagtgacaa tgatttgttc cacttcttga
    12121 agcagtgtat ctttgataag ctctcccaag gcataactag aagcaagtcg gatggtttga
    12181 tccgttggtt tatcaagtaa atctaatcct aatacttgtg cagattcaat gactgcggta
    12241 ttttttcctt gccagttcgt caataaataa ctgatttttc gctgacgaaa taagagatcc
    12301 atcccatcaa tcgtacgcca ggttcccccg agcgcgtaat agattgcttc tgcagtattt
    12361 tctccgccat ctgcaatcgg cacattgatt tttcttcctt tacggtcttc ccaaccattt
    12421 agaactgcct gatttaattc aaatgaagaa gcacttcctt taaatgaatc gattgccgcc
    12481 aaaatatcca tctatatccc tcactttctt cttaagtaag acactttttt tcactttttt
    12541 caaacaaaac aatgctattt tcttctgttt ttctatatac taaaagggaa ctcattgaat
    12601 aaaggagaga aaaaacatga aaatcgcaat agctggagcc ggagcaatgg gcagccgaat
    12661 cggattaatg ttgcatcaaa gcggaaatga ggtcgtcttg atcgatcgct ggcctgctca
    12721 cattgaagcc attcgtacaa atggattgat tgctgacttt aatggcaaag aagtagttgc
    12781 aaaactgcca atctattcac cagaagaaat catcgaaagc aacaaacacg tggatttgat
    12841 cgtagccttg acaaaagcga atcaattaga tgatatgttt cgttcgatcc aatccattat
    12901 tactgagaat acttatgttt tgtgtttact gaatggatta ggtcatgaag atgtactgga
    12961 aaaatatgta ccaaagaaaa atatcttatt tgggatcacg atgtggacag ctggtctagc
    13021 tggtcccgga aaagtcacat tattaggtga tggagaaatc gaactagaga acttggaacc
    13081 agaaggcgaa gcatttacaa aaaaagttgt agaagtcttt caagaagcaa aattgaatcc
    13141 aatctatagt cataatgttc gttattccat ttggcgaaaa gcttgtgtaa acggaacatt
    13201 aaatggatta tgcacgatat tggattgtaa tattgctgaa ttaggtgcgc aaaaagcggc
    13261 agagggcatg gttcgaacga ttgtatctga gtttgcttct atcgcagcaa aagaaggaat
    13321 cacattagat caagaagaag tgtaccaaca tattgcttct acttatgatc ctgacaacat
    13381 cgggttgcat tacccatcta tgtaccaaga cctgatcaaa aaccatcgtt tgactgaaat
    13441 cgactacatc aatggtgccg tttggcgaaa aggacaaaaa tatgatattg ctacaccata
    13501 ttgtgccttt ttgacccaat tagtccatgc aaaagaagga atccttggcg cagaataaaa
    13561 ggctctctga tgagaatcag ctggatcagc agctggttct cattttttta cccccatgaa
    13621 agttcaaaaa gaaaaacaca attctcatgt tgccataaga attgcgcttg tttattttca
    13681 tctatgattg gttggcactt ttatttattt ttaacagagg aaagatcgta ttcatattgt
    13741 tcataaatag cggagtcagt agtgaaaaaa cgttttgccc aattttctgc atcaaaaaat
    13801 tccaacagtt ctgatgggat tggttgaaat ggcgtatgca aaaattccgg taattcatct
    13861 aaatcacgtt cttgaagcaa aaaaatattt tcagaatgat aaaactgata atgagctatg
    13921 gcttgattat ctgtcactag tttcttttga aaatacatag attcaaacgt tcgcaaagtc
    13981 actccttgct gcccttcccg caacagttct agaatccccc tcgattcccc ggtttttttc
    14041 agatattctt cataagtaag gtatccaggt tgttcatttc ctcgaagcgg aagaataaag
    14101 taattggtcg taacacctct ttttgtaaat tcctgcttat atttttctgc ccgttctttt
    14161 ctgccgttat ttgttgcacc aaaaaataga tccgtctttg gttcgctaga agaaaaatcc
    14221 atatgtttcg aataaaagga actattatgt tttagtccaa gttctttcgc ttctaaaaaa
    14281 tcaaaatgat aaatctcatc gattgccggt tcatcttgtg cctgcttcag catcgcctcg
    14341 tgttctgctt taaagtggtt ccaaaaaaat aacacgattt ttgcattggt aaatggacgc
    14401 acgcgtcgag ccatctccat atcaaatcca taatctaaaa aaacgatcag ctgataattt
    14461 gccaactgtt gtcgagctga tttaatgaat tccgactgta gtaaatcttt tccggaccaa
    14521 acatctaaag agcggatgga aggaaaaaaa taaagtgatt tatttttcgc aattagcaat
    14581 acgtcgtttt tgttcatctg ttccgcctca ctttctctgt cactttttcc tatgatagcc
    14641 tgtttaaata gtaaaaagcc agtaaaacgc ttcctattat ttttataaaa atttaacatt
    14701 tctatgaaaa tacgatgaaa taaatcattc ttccattaaa atatgcccaa aagttttctg
    14761 aatagttgta at
//
LOCUS       DAZUMV010000061        14770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000061 DAZUMV010000000
VERSION     DAZUMV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.061"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..777)
                     /locus_tag="ACG8FK_001636"
     CDS             complement(151..777)
                     /locus_tag="ACG8FK_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097245.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1072..1407)
                     /locus_tag="ACG8FK_001637"
     CDS             complement(1072..1407)
                     /locus_tag="ACG8FK_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5097246.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1394..1678)
                     /locus_tag="ACG8FK_001638"
     CDS             complement(1394..1678)
                     /locus_tag="ACG8FK_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS5097247.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIVTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1734..3257)
                     /locus_tag="ACG8FK_001639"
     CDS             complement(1734..3257)
                     /locus_tag="ACG8FK_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5097248.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3250..4425)
                     /locus_tag="ACG8FK_001640"
     CDS             complement(3250..4425)
                     /locus_tag="ACG8FK_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5097249.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4429..4614)
                     /locus_tag="ACG8FK_001641"
     CDS             complement(4429..4614)
                     /locus_tag="ACG8FK_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097250.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4580..6274)
                     /locus_tag="ACG8FK_001642"
     CDS             complement(4580..6274)
                     /locus_tag="ACG8FK_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5097251.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6271..6744)
                     /locus_tag="ACG8FK_001643"
     CDS             complement(6271..6744)
                     /locus_tag="ACG8FK_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS5097252.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6813..6968)
                     /locus_tag="ACG8FK_001644"
     CDS             complement(6813..6968)
                     /locus_tag="ACG8FK_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFS5097253.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7102..7482)
                     /locus_tag="ACG8FK_001645"
     CDS             complement(7102..7482)
                     /locus_tag="ACG8FK_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5097254.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7486..7701)
                     /locus_tag="ACG8FK_001646"
     CDS             complement(7486..7701)
                     /locus_tag="ACG8FK_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097255.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7704..8111)
                     /locus_tag="ACG8FK_001647"
     CDS             complement(7704..8111)
                     /locus_tag="ACG8FK_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS5097256.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8377..9852)
                     /locus_tag="ACG8FK_001648"
     CDS             complement(8377..9852)
                     /locus_tag="ACG8FK_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS5097257.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9842..10690)
                     /locus_tag="ACG8FK_001649"
     CDS             complement(9842..10690)
                     /locus_tag="ACG8FK_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS5097258.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10727..11089)
                     /locus_tag="ACG8FK_001650"
     CDS             complement(10727..11089)
                     /locus_tag="ACG8FK_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097259.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11090..11248)
                     /locus_tag="ACG8FK_001651"
     CDS             complement(11090..11248)
                     /locus_tag="ACG8FK_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097260.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11389..12013)
                     /locus_tag="ACG8FK_001652"
                     /pseudo
     CDS             complement(11389..12013)
                     /locus_tag="ACG8FK_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12010..12321)
                     /locus_tag="ACG8FK_001653"
     CDS             complement(12010..12321)
                     /locus_tag="ACG8FK_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097261.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12365..12658)
                     /locus_tag="ACG8FK_001654"
     CDS             complement(12365..12658)
                     /locus_tag="ACG8FK_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097262.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12852..13499
                     /locus_tag="ACG8FK_001655"
     CDS             12852..13499
                     /locus_tag="ACG8FK_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5097263.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13560..14705
                     /locus_tag="ACG8FK_001656"
     CDS             13560..14705
                     /locus_tag="ACG8FK_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5097264.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
BASE COUNT         4266 a         3041 c         2347 g         5116 t
ORIGIN      
        1 atgagaaacc catatcaaag aactataaag gttatcaaaa cagcgtttat gactaaatga
       61 attagagtta tctatcacta tttttcaagc gatttaaacc aaatggcata caatatggca
      121 tacaaaaact tcaataagcc gtgggggttt ttaaatttct tcttgttctt taaaatcaat
      181 gagtggagct acataatttg aatccacttt ttcaaacgtc actttttgca tcgacgaatg
      241 ttttctttcc caagataacg taaatagatt tgactctgga cttataaagt ctacttcacc
      301 ttctagtatt actttcttat gcaaatattc ctctaataaa tatagacttg ctaaagcata
      361 caacacattt tccaaatttg cttccttata atttgaagaa cgaccatgtt ttactctgtt
      421 atatattttc caccaatctg gtgtatcttc aggccaccca tctccaaaag gttttagtga
      481 agtcaatgtt aaagaattca taaccctaac accattatcc aaacacatta attcgctaga
      541 tttaataaca gctttaaaat cagttatgct tttattgcta ctgctatcaa atccgcaaag
      601 ttctttgaaa ataatctcac actccgaacc aatcgctagt agtagttttt gatattcaat
      661 agagaatgtt ttgaaattat tctcatcgag catcacaaaa tctgtggtat taataaactc
      721 attttctaaa tgtagataat acttccaata aaaatttgta aactctgctt tgtccattaa
      781 atcacctctt ttgaattata acaaaaaaaa cacgtccaca acagacgtgt tctttgattt
      841 ctacacaatc agcttgccaa aaccatccaa tcagtcctag catgcctgta ttaaaattga
      901 acgttcatca tctatcatct ttcttagtcc agcatgtttg caaccacacc tacaattttc
      961 agtccaacaa tagatttcga ctagctaagt cttgcaaaat tcaggcagct aacccttcat
     1021 ttactacctt aattatacta aatacccaca acgtttacaa gtgtaatcat tctaagaaat
     1081 ctgtttagct atgatcgttg tataatcttt aaacgccgtt ccttcattga ttgaaataat
     1141 tttataactc ttgtttttcc aagctacttt catatcgttt gttagttcat aatcttgatc
     1201 gtatcgaata ataaacgtca acgttccttc aagaatggta cctacactag caagcacgtc
     1261 ttttagcatt tgtgaacgca cattagccca acattcaaat acagtttctt cactatccac
     1321 catctgccca tcttcatctt ttacccgttt tgtcttttta aacttgactc tctcagtcaa
     1381 ttcatttaca ttcttaatga ttggcataat aagcacctct catttgttgt actaatgctt
     1441 gtaccgttac tggtatccgt tcgctgctgg cttcttgacg gttcaaatac caatgttgag
     1501 taagtaatga caccgccaaa tcaaattgct gataattatc catatcttta tccgttgcgc
     1561 tactatcaat agcacttttt atatattcgc ctgctgtaac aattaattgt tgaataaagt
     1621 catcatcaat agtgtgatcg atccgcatac ttttttttat gctatccaaa attaccattt
     1681 tatctctcct aatgaaaaaa aggcagtggg ataaaccgcc gcccaatatt tttctatact
     1741 tttggtgtta caactggcgt aaactcaata tacactgaag cattttgatc aattttctta
     1801 taatcgttgc gaacgatcac tgcaagtcct tgtgagtagt aatcgaattt ttcccattga
     1861 gtcgttactt gatttctacg agcaacaaag atagattgtg caatatcccc cataatcata
     1921 gggaatgttc cagctttagg attggcaaac aatgtatctg caatcaatac tactggcata
     1981 ccgaataatg atttaccact aggtgctgtc acatcgggtt gtaaaatgta acgtccatct
     2041 gaatctttca atgtatctag gtggtaatat ccgctttggt ttagaattac cattttattt
     2101 aatgctgggt ctaatgccac attgtatagt tgtttcaaat cgtccaacgt agcagccgtt
     2161 ttcttagtga atgtttttaa taaatccatg atatgcttat tgtcagtgtt ttctaccaat
     2221 ttcgctaact gatctttgac ctcttgtaca atattcactg ctgaatcttc cacaacctca
     2281 tttgataagg caatcttacc agcacgagtt tctactttat aatcaactga agtaaacatt
     2341 tcagcgtcaa tatcaccaat ttcagcaagt tcagcttttg ttgctaacac tgcttgttgg
     2401 ttagttgcta ctggatattt accttgtcca tttgatactg tttttactgt tgcatattga
     2461 gctaagttat aatttgaacg tttcaaatca aagacttcac cgattacttc ttcgggaaca
     2521 actgccgctg catttacagt agtgactcca tcacgcactt cgccttgtga acggatatat
     2581 tcttcatacc cacgcaattc tgttttttcg ttatcgatta aagttttttc catgttatta
     2641 ctctcctttt gtgctttaga agtggattct aagctattgt ttgtttgttt tgcttggttg
     2701 ttactcataa acgattcgta agaacgctta tttacttgga catttgatga atcataagcg
     2761 ggaacggtca cgacactaat ttcattaagt gctttgattt tatctattga tcgttcaatt
     2821 gtgccatctt cttttttgtc gaatgaatct aatccaagca cgaacccaaa ggacatagaa
     2881 tcaacaaccc cttttgagat attctcatac acatcatttg aataggtcgt gtcggttagc
     2941 tctgcttcaa aatgtaatcc tgtatcatcg acattcaatt tcaacgtacc tgctttgaca
     3001 ctagctagtg gctttgaata atcgtgtcca tataataaga acacatttga taaatccacc
     3061 tcttttaatg cttcgggtgt aatcacttct acaaagccgc ctaaatcttt tgacggttgc
     3121 ccgaatttca acgcatagcc tgaaatagtt ttgccgtctg ttggttgttt ttcattttct
     3181 ttctctactt tgggagaatc ggctgttagc tcagcttctt ctgtcagccg tttttccttt
     3241 tcttcttcat tattcaactg gtgtaactcc ttcctgttct tttaattgat atttctccaa
     3301 cgtatctaaa tacgtaaagt tcaaacttgc tagtaaacga tcgccaccat ccatgggggg
     3361 tagccccatt ttcgaccgcc cctcattaat cgtgagaagt gaccctttga cctgatccaa
     3421 tacatttttt acttttgttt caggatcagt ctctagtaac tgatcggaat taaagcggat
     3481 attggtttca agtttcgtat ctaattcact cacaaacaca ttaaagtaat gaatcagcgt
     3541 actttggata tactggagat tactttgaac ggtgctagaa tgttcatttt caacgcctaa
     3601 acgctcaatc ggtacaccaa acgccttggc aatttgtttt gtgttccaat cattggaatt
     3661 gataagcttc aaaacatcgg tgtttatttc taatgttttg tagtccattg tttcatcaag
     3721 aatgatcgtt ctaagagcat tatctccact acttgaaccg ttggcttctt caaacttttc
     3781 acgaattgcg ttctttgctg atccgtctaa atctgactta tgaaccttca aaatacctga
     3841 cccactgaca ccacgagtaa agaaattgtg caatgtgcgg ttgccagctt gttgaatttt
     3901 cagttcatca cgtaacgcat aaagtggtgg taatcctgtt aaaccatctt gggtgaaata
     3961 tttgaagtgc aaaatatcgc tagacttcac acgtcttttt ttatcaccaa tctgataaga
     4021 caacgtacca ttatctaatt gagtaaccgt tacacttgag ttaggtaata agtgaatttc
     4081 ttctaccttt tcaccgttgc gtttaatctc tgcaaagcta ttaccgttta gcaacatatt
     4141 gaccgccaaa gcaaacttta aatgccaacc atccatttct gaattaggtt tctcgtttag
     4201 taacttcacc agttcattat cagcttgtgg catattgttt ttgaccaatt gaatcggact
     4261 tgaagcaata tcactagcaa taattcgaac ggctgcaaac acatcactat ttcttaatgc
     4321 acgaacgctt gtaaagctgg tggtgtaatc atccgattgg attgatacca catgttgtag
     4381 gaaaggatct cccgttgtat tttgtctcgc tttaaaaaat gccatctttt aacctccctt
     4441 ctgttctgta tttagcatga ccgctacccc aataagcact atgcccagcg ctaacaagcc
     4501 atagtagaca tttgttagta aggtgatagc aaccaacaca cacaccaatc caagaagtaa
     4561 aagaacggta tgaatgtaat tagaaagaaa attcttcact tgtataaaat tcattgtctg
     4621 ctttcgctcc ttccgttgtg gtgaagtaat ccattgcaaa cacataggca ttgattaatg
     4681 cagctattgg atcaattttg ttactgttct ttgatttatt aatctgaatg ccgttgttat
     4741 ccgtttttaa aatagcgttg ttcactgcat gggtgagaat cgtattttta ttgtgaataa
     4801 cgttgccttc ataaacttgt tctctaaaag ttcgggtcgg aacgtttaga gtaatcgttc
     4861 cttgtcttac ttctaacatt gggtagttgg ctttttcagc tttactgata agcgaattag
     4921 cgttatacgg gtcataacaa atccccatac attccaaatc attttcttga atcaaatctt
     4981 gaataaactg aaatacttgg tcatagtcca cgattccact ttctaattga gtaatggaac
     5041 actcaccagc tttttctagt tctcgataag gcaaaccgtc gcgcttttct ttgtcttgaa
     5101 gcccatattt ggtggctaca aagctatgtg aatcacaata aagttcgccg ttatctagcg
     5161 gtacgatcca tgaaacgcta gttaaatcat ctgttttaga taaatccact ccgatataaa
     5221 cgggcttgcc tgtaatatct tgtggttcta cttcggttgc ctgccagtct tcagcaatca
     5281 tatagctgtc ctcactcgct tgtctccata aattgaagtt tttcaccaat acagaattaa
     5341 ggttattttg tttcaaagca aggttcacat catcttgaat ggttggaatc attacttttt
     5401 tgatttcttc actttcaaaa atcggattgg ctttgatcca tttctcttga tcgtgaattt
     5461 cttcgggatc gtccaattcc catatcgcta taaagtacct gtcggcttgt tcttcgcctt
     5521 ttagcacacg atctaacaat aagtattcct cgtacatagg aacgtttaaa tcaagcccag
     5581 cggtactaat aacacacaat aagccgttct tttgttgggt cattcctgac ttaatgacgt
     5641 tgtacgtttt acgagttttt gcttcgtgcc actcgtcaag aattgcggtt gttggtgcaa
     5701 acccatctag cgtgctggtt tcactagcca gtgccatagc gaacgaatta gagggcaagt
     5761 gctgaatctt agagttcata attttcagtt gtggtcttaa aaacttacta gacttgacca
     5821 cgtttctaag tgaattagac agcatatcat agcccaattt agcttgtttc aaagcgttac
     5881 ttacaaacaa tacttgcctt gcttctgctg gttcttgttc cataatcaac gcattggcag
     5941 ccatgccact tgcgatatac gttttcccat tcttacggct catactggta aacgctcgat
     6001 tgaatcgcct gtaattccct tctttagtac gccagccata aagactgcct agaatgaatt
     6061 tttgaaatcc taacatcttg attgctttac cgtccgtaga tggtagcaac tcaatgaatt
     6121 taatcgcttt ggtggcttgt tcttcatcga acacataagg gaagctatct gacttagaac
     6181 gctccaaatc gcttatatga cgctcacacg cttggataat tttcttgggt gcaatcagct
     6241 tacctgatag gactttctca atgtaatcat tcatgaattc atcaactctt tgaacgggtc
     6301 ttcgggttca tcatttttaa ccttgttcaa agctagtttt gatcgtgatt ctaaggtcat
     6361 acctagtgag gtggcttgtt ttttcaaatc actcatagct tgtgattgaa cccgaaccgc
     6421 tggattggct ttctttactc cgttttcgca tgtgataaac gtacctgatt ttcttatctc
     6481 ggcttcggct gttttcattc gagcaaaggc taaacaatag gctaccaatg aaccataatc
     6541 tgcttcactc aatggataat ctttttttaa tgttggtacg atacgattcc attcacttct
     6601 agcgctcgct gccatccagt cagggggcgt gatatttatt aatggttgct gcttgaacaa
     6661 ttcttctcta gcgtccacac ggattgcttt ttcttcactt gatatattgc cttttgtatc
     6721 gtgtaatatt ttagggtgtc ccatgttgtc ctcctttcag acaaaagaaa aaggaacgta
     6781 agcaattaag ctcacattcc ttcgtttttc gatcagaatt tttctttatc taatttatct
     6841 atataaatga tttttccgtt ctgcatttga atggtcactt gaccaaaatg aggtaactca
     6901 atggtgcgct gtttccctgt tttatcagta ataacaattg tatttttcaa taaatctcct
     6961 actttcatta tatacttttt cacttgtgat tacaagtgta atcgttattt gtaattaccc
     7021 ttatgccttt tcacaacggt tatttgtacc aaaatgagta ggcatcgatt gtcagtaagt
     7081 ttcacactat gcccccttaa ctcaaagggt actcttttct cgtctttgtc tttcattggc
     7141 tgtcttggaa ttatggtgtg catggcataa agaacgaaaa ttagtttcat ctaaccgtct
     7201 attccaatca tcttttagtt caattttgtg atctacaaca tcagctttcc ttatcaagcc
     7261 ttcttctagg caatcctcac atactggatg atttaaccga tacaagtatg aagcctttcg
     7321 ccatgttcta ctttgataga attgaaaata tttaccttcg ctttgctttc tttcttcacg
     7381 cttaattgcc gtttgctttg ctttatgctt cccacaatac ttctgcctat aatcaatcaa
     7441 tgttttacat ccagcatgat tacattgttt tttaactgcc atagattacc aaccaaagcg
     7501 atctcgttcg tcagaatcat cttgttccca ttttgatttg tattcaatat aaggcgaaac
     7561 tatcttcaag cacgttccgt ctttaaaatt aactttagtg attttatctt cctcaaaact
     7621 aataaagtct acatcttctt gagtgtttga atactcaatg tgtttctcac catcgaagta
     7681 tctaatactt tccatatgca taattattct acctccaata aattagttat caattcatca
     7741 tctgttagta gcaaagccaa gaacttatca tggatgttta aaaacaaatt atcctcattc
     7801 gatgtaagct ctcgttgtct cgctcgatca tataggttaa gcagacaatt aaattcatcc
     7861 attagctcac ctgtatcatc atagttgcca ctatctacca atgatcccac tactagcgat
     7921 tgacaccacg ctactgactc ctgcagccaa tcaggttctt cttccattat ctttaatact
     7981 tctggatcaa atgtggaata atcttctgtt accaatccca cactaaatgc cagcgtgttc
     8041 attagaaacc gatccatttc atctgtatat tgaaagttcg gtgctttgtt tgttgaaata
     8101 atattgagca tttactttcc tccttgattc gtaacacgtg gtaacaccta cgtaacaggt
     8161 acccgttacc actcaatccc aacggggaca agggttctag cgttttggta acaggtaacg
     8221 ggtaaaatcc agtaacatta ttccccagca tattagctaa ctatttcaaa agttttaagg
     8281 aaataaggtg ttaccccgtt accaaattgc tgtatccctt ggggcagtaa gggtaaacta
     8341 gtaacgggct aggtgttact gacccgttac tacccgttac tttttgtact ttccagctcg
     8401 atttttattt tcataggcat ttgacttatt gtaaaatcct cgtcgtttat ttctttttgg
     8461 cagttgcttc cgttcccaac caggaatact gctaataatc aaggcaatct ttttagtttc
     8521 tgcatctgat cgaccattca ataaatcctt tgcttgttta tcaaaaacag cttccaaaat
     8581 atcagcggtg agtacgctat caactaaata aaactcttgt tgcatagtag ctccattata
     8641 gaaatgctgt aaatttttct cattacattt actgtcagga tagttctgat aataagaacg
     8701 tctcgcccac gctggagctt cttcccattt atacgggatt tccatttcca aatatttagt
     8761 gattgcttct ttaattggat tctccatctt ggcatcctct tgatagtctt ttgcaatttc
     8821 taagacttct ttatcttcat cgggactaaa atagattcgt tgtcctttat caaaaagcac
     8881 ctttgcttca gctaacactt gaagaaaata ttcatccttt gttttaaacg gattcatctt
     8941 tggcttattc ttacaaggca aaggataaaa acggcggttt cctgtatcat ctttcaaata
     9001 ctgcccatca ttcgacgttc caataaatac acattgcctt gcccaagcta tgacattccg
     9061 accataagga ggtctaattt tatcgatctt ggcactgatg aagtttttta tctgttcaat
     9121 tttagttttg ttcattgaag ataattctcc aagttcaata attacattgc cttgaagctg
     9181 catataatca tctttctttt cacctagcga atctaatgaa tcggtgaaaa aatcatccgt
     9241 gtaaagtaat gctggaacgg tactcttacc aatcccttgc ggtccatcca aaactggaac
     9301 gatctcaaac tttattcctg gtttatagat acgtgcgact gatccgacta tccaacgttt
     9361 tgttacttct cgtgtgtaaa ggttgtcgtc tacccctaaa aggtcaataa acagtgtttc
     9421 cagtctaggc tgttgatccc atttaacttt ttcgatacgt tcttttactg ggttgaaaac
     9481 attctttcta gccacattta ctactgcttt gtgtattttg ttgtcatcaa aaactacatt
     9541 gtagttttct tcaatatatg ccgttaaggc atcctcaaaa ctgtcttccc attctccagc
     9601 ggtacgattg atccaaggag aatcttttaa taggtgaatg tatccgctaa attcgttata
     9661 agcaattgtt ccagctaaat tgctatcatt ggttaaaatc aattctgcat tcacaacact
     9721 gttactttta atctgatcgt ctttattgca tcttaatcgt gacatccagg ttttcaattc
     9781 aatgatattg tcttcacgct cgctggcttc gggaaagtct aaaatactat ctaatttatg
     9841 cttatgagcc atgagctttc cccctcaaat cttttttaat catcgatcga ataatttgat
     9901 tcacttcttt atcttttagc ggtggttcat tgtacgcatt tgctgtataa atcaattctg
     9961 ctaaagtttg attatctaca ccgtgccata gtaaccaacc gcataactta gttaaatgat
    10021 cgtttctact accttcgcca gcgccttgaa caatcatatc taattttttt gctgtccctg
    10081 tttttgctac gggtcggtat tgagtcttta tttcttgcgg tttttttagc agtaggttca
    10141 ctagccaatc gggagctggt gcaatattat ttagagatac gttttcaata ggttcatatg
    10201 ctctcccaac acttcttgaa ataatcatag ttggcgctat tggaactcct ttagtgataa
    10261 tatcaatccc gcttaaactt ttacctttat catcacgccc gaagatatca gcaaatgcac
    10321 ctgtttcttg ttggataatt acaccatcag gaattttaaa gaagaaatgg attcctttac
    10381 ctggtgtgat ttccgtataa gtagaaggaa atggatcata cattgaagca agttttttat
    10441 aattctcaac gccgtttcgt gtttcagaat gttgatctag gtcaactacc accatttgat
    10501 gatcttcaag cattaaccca atatttctag ttgcatcttc cgtccaccat tcattaattt
    10561 gattagaatc gttagttgca tcattccaac cactagagcc ttttattgga attttagttc
    10621 caggagataa gggataaatt gaccatcccg acttttgtaa ttccgttgct gcttgataat
    10681 tatttgccaa taaacctctc ctcctttttc aaaatactgg tataaatcat tcagacacct
    10741 ccatgagctt caactgctca agttcgtatt tcaacccatc ccgaacaaat tcagcacctt
    10801 ttaattgatt ctgatacttt acccatgtat taaatgtttt cacccttgct tcttgcatat
    10861 cagtaatgtt taaccatgcg gttaaaatct gattttttag ctcgttgatt tctcgacaaa
    10921 ttattttgtg ttctgcatgt tttttgagta catacaaaac tgttagtttt tcattgaatg
    10981 aatttccaaa gtaggttttc ccgatattat tttctagttc agcgatccct tgattaattt
    11041 cctctaattc aaggctatcg agttggttta atagttttgt tttgtacatc tacttatgcc
    11101 ctccagttct taattgatag tcgtactcgt cataagcagt gaagagatat aacgctaatc
    11161 ctgcaaagat agctattttt gcaattaatg gtaataagcc gaaaattccg ccgattaaaa
    11221 tgcttcctat aattaagttt cgtttcataa tggttccctc cgatttttta atatggtata
    11281 atcggaacaa agaatatcgg gttttatccc aattaagttg cctttatcct gtccgccaag
    11341 actgttatgg ataaggcttt ttctttgttc tcgatcatgt taatgctcct aagttgtctg
    11401 caattcagca gaacgattat aaattttctc actcactgaa aactgcaacc catatttttc
    11461 tttaacagtc attaattcta ctgtctcatc taaaataggt tcacgatcaa ccaacatttc
    11521 aggagtcata tctttctttt ttaccatttt ggaatgacca taaatagttg atacagcttt
    11581 attggctatc acattcgctt taatgtagtc tactggtttt ggttcagtaa tagcgtggct
    11641 taatttagtc attgcttctt tttggtgttc tttgtctagc atgcggaata cttgaaagcc
    11701 ttcaagtcct gtggactggc gtagttcttt gatgatgtcg aaaatccagt cttgaaatgc
    11761 ctcggcttct tttttacggc ttttgaatgc catgcggtaa atattttttt tcgttgatga
    11821 tgtttacatc ttgtgttctg cccaatgaat cgatgacctt actagtagta accccatctt
    11881 taggcaaaga gtgaatggct ctagtaacct gtttaagacc taacgcttcc gcaacatctt
    11941 tcgccactgc ccaccattca tcattaatat cgacaaatct tataatgtgt ccgttccaaa
    12001 tttcagtttt cattcaacgt cctccagttc aagccctagc aagctcgcta acaactcaac
    12061 ttgctgatac aaaatttctt gatactcttc aatagaaagt cgttctttaa actctgcttg
    12121 tactaaatcg ttcaagccac gcaatactcc gcttaccgtt ggttcttgtg gtagcaattt
    12181 ttgatagtcc atttgttcgt acaaacattc aaacgtttct tctagcgctt tgccttccaa
    12241 gtttgctaat tctgccgctg tgtcttctgt aagaattgtt gataatactt tttcgattgc
    12301 tttattttgt gtcgattcca ttattatttc ctcctattaa tccctttgcc cccacggctt
    12361 gcgtttattt ttggtattct tccataaagc gatcacattc atcttgtgaa attcttacga
    12421 ctccatcaac tataatcaac tttaaaccgt taggaacata tttctgcatc atagtattgt
    12481 atgaaacgtt catatactta gcagcttgtg ccttgttcat atattttgga aactggtact
    12541 ttttttctaa taatgaaagt aacgttcctt gagtttgctc tacttgttga acaactcgat
    12601 cgtcaataat tttaaaagct ggatcatcaa gcgtgagttt gtcaattttt aattccatat
    12661 ttatcccccc tcgttagcat acatttctat acaaagattt taaaaaaatg caagaggctc
    12721 ttgaatacat ttctatacct cttataaact caatatacca tcataccttt tcatttgcaa
    12781 gaattttaac atactttttt gaacttttat gtttcacaca tatttaaatc gtgatatagt
    12841 agagaaaata tatgaatggt ggtgaaaatg tggctagaaa aaaatacccc gaagcagtgg
    12901 agacaggaca aagaattaga ctggctagaa ttgaaaaggg actctcaatg gaacaacttg
    12961 gaggtaaatt atccccaccg gccagtaaag gtgccgttag taattgggaa aatggctata
    13021 atcttcccaa taacaccagg cttaaacaac tttcaaaggt attagatgta tccactacct
    13081 atctattaaa cggatcttat acattaagag atatgcatat gatgcccgaa attaagcgaa
    13141 atgtgcttga aaagcaattg aaaaaaacta ttgaaaaaaa taactctata attgattcta
    13201 atctttctaa tgctatcaat aacatcgaag aagataacct gactgtctca cagaagattt
    13261 atttaacaaa cgcaataaaa tatcttaaaa caggatctcg aattgaattt cctgacggga
    13321 gtaccccaat tgcttatctc gcagctcttc taaatacgct tttacgatat gactcattaa
    13381 ttaatgattc agaaaaaaat acagatgaga aaaaagagta tcaagaattt tatgaaaatg
    13441 atcttctaaa agagttcaca gctcttgtta ctgagctaaa taaacactat tcaaaataat
    13501 aataaactgc ctcctccaac cgcccccacg gcttgctaca ctatggaggg aaaactatta
    13561 tggcaacatt caagcaatat gaaactaaaa aaggtaaatt ttggctctat gaagcctatc
    13621 ttggaatgaa caaaatgact ggcaaacctg ataaggttcg tagacgaggt tacaaaacaa
    13681 aaaaagaagc ccagcttgcc ttgtcacgct tgcaagtgga ctacgataaa aacggtatta
    13741 aaaaagcgaa caacgaaacg tttaaagaag tttatgactt gtggctagaa acatataaaa
    13801 ccactgtccg agaagccacc ttcatgaaaa ctgaaatcaa gtttagaaag tggattcttc
    13861 caaaatatgg aaatcttcgt gtgaatgaag tcacggtaaa aaaagcccaa caaattgtaa
    13921 atagctgggc tgaaactact gaccaataca aggtacttca ttcaacttct aagcgtattt
    13981 ttagctacgc tatcaatctt gggattattg agactaatcc actagacaac gtattaatgc
    14041 ctaaaagaac caccactaag cataaagaaa aggtcaaagt ttacagtaaa gaacaactga
    14101 tcaccttgtt tgattaccta aacagcaaac ctgaaactta tcgaaacgac tttgataaaa
    14161 cgttactacg ctttctcttt tatagcgggg ttaggatcag tgaagccctt gcattgaatt
    14221 ggtcagacat tgattttcaa gaaaatacgg tcaccatcaa taaaacacta agccaatcca
    14281 aaaatggcta taaaatcagt gatcctaaaa ctgattgtag tgctggggtt cttccactag
    14341 atgaaagaac aatttctata ttaaaaaaat ggcagattaa tcagcggaaa tatatgctga
    14401 cacttggtgt aactgatccg actatgattt tttgtggtat ctataaagaa ctaatcacac
    14461 accacgctat ttatgcaaga ttgattacta tttgtgaaaa agctatggtt ccatttctcg
    14521 gtgtgcatgt agcaagacac actcatgctt caatgctctt ggacgctgga gctactatgg
    14581 tagaagttca aactagatta cggcatacaa aaattagtca gacgatcgat tgttacggtc
    14641 acttggcaaa agaaacgaaa gaaaagactg ttgaaaaatt agtacaacac ttaaatattg
    14701 actagatggc atacaatatg gcatacagta aaaaataaaa aaaggagaaa ccctatcata
    14761 tcaaggtttc
//
LOCUS       DAZUMV010000062        14768 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000062 DAZUMV010000000
VERSION     DAZUMV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.062"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..485)
                     /locus_tag="ACG8FK_001657"
     CDS             complement(<1..485)
                     /locus_tag="ACG8FK_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS5097265.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     C"
     gene            758..3229
                     /locus_tag="ACG8FK_001658"
     CDS             758..3229
                     /locus_tag="ACG8FK_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5097266.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3332..5068)
                     /locus_tag="ACG8FK_001659"
     CDS             complement(3332..5068)
                     /locus_tag="ACG8FK_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5097267.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5065..5769)
                     /locus_tag="ACG8FK_001660"
     CDS             complement(5065..5769)
                     /locus_tag="ACG8FK_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5097268.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5900..6403
                     /locus_tag="ACG8FK_001661"
     CDS             5900..6403
                     /locus_tag="ACG8FK_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS5097269.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6363..6701
                     /locus_tag="ACG8FK_001662"
     CDS             6363..6701
                     /locus_tag="ACG8FK_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS5097270.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6722..8140
                     /gene="ffh"
                     /locus_tag="ACG8FK_001663"
     CDS             6722..8140
                     /gene="ffh"
                     /locus_tag="ACG8FK_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS5097271.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8252..8830
                     /locus_tag="ACG8FK_001664"
     CDS             8252..8830
                     /locus_tag="ACG8FK_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS5097272.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8834..9355
                     /locus_tag="ACG8FK_001665"
     CDS             8834..9355
                     /locus_tag="ACG8FK_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS5097273.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9434..9988)
                     /locus_tag="ACG8FK_001666"
     CDS             complement(9434..9988)
                     /locus_tag="ACG8FK_001666"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS5097274.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10241..10516
                     /gene="rpsP"
                     /locus_tag="ACG8FK_001667"
     CDS             10241..10516
                     /gene="rpsP"
                     /locus_tag="ACG8FK_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS5097275.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10528..10779
                     /locus_tag="ACG8FK_001668"
     CDS             10528..10779
                     /locus_tag="ACG8FK_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS5097276.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10904..11695
                     /locus_tag="ACG8FK_001669"
     CDS             10904..11695
                     /locus_tag="ACG8FK_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097277.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11865..12386
                     /gene="rimM"
                     /locus_tag="ACG8FK_001670"
     CDS             11865..12386
                     /gene="rimM"
                     /locus_tag="ACG8FK_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS5097278.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12376..13137
                     /gene="trmD"
                     /locus_tag="ACG8FK_001671"
     CDS             12376..13137
                     /gene="trmD"
                     /locus_tag="ACG8FK_001671"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS5097279.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13273..13620
                     /gene="rplS"
                     /locus_tag="ACG8FK_001672"
     CDS             13273..13620
                     /gene="rplS"
                     /locus_tag="ACG8FK_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS5097280.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13656..13841)
                     /locus_tag="ACG8FK_001673"
     CDS             complement(13656..13841)
                     /locus_tag="ACG8FK_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097281.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            13878..14171
                     /locus_tag="ACG8FK_001674"
     CDS             13878..14171
                     /locus_tag="ACG8FK_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS5097282.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(14259..14405)
                     /locus_tag="ACG8FK_001675"
                     /pseudo
     CDS             complement(14259..14405)
                     /locus_tag="ACG8FK_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         4908 a         2676 c         3009 g         4175 t
ORIGIN      
        1 atacacaaca ccgtcggaag gaagttcata cgtggctggt aatgcttcgt tgcttcacgc
       61 aagatccgtg tcaccgtccc atccgagacg aagtacttct tagcgattag tttacgtgat
      121 tgattttcgt acagttccat gagaatcgcc tgcttcagtt cctttgttag ttgatgatgt
      181 tcatcaacta agtccgtttt cgcagaaaag gtccttccac aatcatggca gagaaaacgg
      241 gtacgtttta attctaaaat agttaacttt tctttgactt tcaacatctg agtgcgtgtc
      301 gtataggaac cgttgcggat aatacgaacc gatcgacagt gttcacaata ggaagaagag
      361 tcatccaatt ttcctttaat aaactgcgct tgaacgcctt tatattcttt ttcaatcagc
      421 cattcttcat caaaaaataa atgtttatct gttaatccga gtaattttct agtatgatta
      481 tccattgaga gactcctttg gtaggatgac gttttggtcg actaaatttt accaaaagtg
      541 agtctctttt tgagttgaaa cgaagaaaaa gtgttagtag aaaatcgcct tcgattttcc
      601 accaacacaa aatattatag aacccttttt ttttgctata tgacaggaat gaaaggtgtt
      661 tgcaagctta atgtgaggaa cactttccat atctgttcta tactggatag gagaagtttg
      721 gaaatatcca gaaaacgaaa tcaatcgagg tagccacatg aagaataaat ggataaatcg
      781 aagtaaaaaa aagaaagtaa cacaaagatc atcagataaa gtcatccgtc tttttccatc
      841 gtataaagaa ggattaacgg cagatcaagt caaggaacga atagaaaaag gtgcagcaaa
      901 taattcagtc gatcccacat tcaaaacaaa tcagcaaatc gtattggaaa acatatttac
      961 gtatttcaat ttgatctttc ttatcttagc cattttgctt tgtttagtag aatcctataa
     1021 gaacttgact tttttaccag ttatcatagc caatacagga atcgccattt ttcaggaaat
     1081 tcgatccaaa aagatcttgg acgaactaaa tgttttaaat acagcaaaag tcaatgtcat
     1141 acgagacggg atagaacaaa caatagatat agaagacctt gtaatagatg acattgttat
     1201 tttagagact ggatatcaaa ttccagcaga tgcagaggtc gtggaaggga aactgcaagt
     1261 taatgaggct ttattgacag gcgaagcaga tgaaataacc aaagaaatag gtgatccatt
     1321 aatgtccggc agtttcattg tttccggaaa agcttacgcc cgattagaca aagttggagc
     1381 agattcgtat atttcccagc tgacagtcaa agccaagaca atgggagaag gagaacagtc
     1441 ggagatgatt gcttctctta atcaattgat caaatggatt gggatcatca ttattcctat
     1501 cggtatcctg cttttttctc aaagttactt ttttaatgga aacacgatca aagaaagtgt
     1561 cgttgcaatg gaagcagcat tgatcggtat gatacctgaa ggactgtact tgctgacgac
     1621 gattgcatta gcattaagtg cgactaaact agcaaagcaa agagttcttt tacacaatat
     1681 gaaaagcatt gagacattgg caagggtaaa tgtcctttgt gtagataaaa caggaacgat
     1741 cacagaaaat aaaatgtctg ttcaaaaaat gatcgtctct aaaaaacaag aaataatcag
     1801 tcagtcagat caattggaag catgcattgc tgactatgca aaagcgatgg caggagacaa
     1861 tgcaacaatg gaagctgtta aagcatattt tacaaaaacc actgatcaat cctatcacca
     1921 tgttattcct ttttcttctg tccaaaaatt cagcagtgta gcgttggcag acaaggtgta
     1981 tgttttagga gcgcccgaaa tggttttgcg cgatcaattg ccggaatatg cggaagagtt
     2041 tgtttctttc gctgaaaaag gctatcgcgt gttggtcttt ggtgtgtacg atgggattct
     2101 tgaagaacca gtattgacag aagcagtcac accattaggc tatattctga ttgctaatcc
     2161 tatcagaaaa gaagcaagag ccacatttga ttacttcaaa aaacagcaag tagccatcaa
     2221 agtgatttct ggagataatc cgctcaccgt atccaatgta gctgtccaag cagggatttc
     2281 acatgctgat cagtacgttg atgcatctaa attatcagaa gaagaatatg aagcagctgt
     2341 cgaaaaatat acagtttttg gacgcgtcaa gccagaacaa aagaaaatct tcgttcaatt
     2401 gataaagcgt aacaatacag tagctatgac aggtgatggc gtgaatgata ttctagccat
     2461 gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca tgcaggcctc
     2521 acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg cagaaggacg
     2581 gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga atattttctc
     2641 ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag aaccatcaca
     2701 aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg ctctagaaga
     2761 aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag ctgtgccagg
     2821 tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga ctttgaatct
     2881 aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag ggttcctcgt
     2941 tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc ttttctgtgc
     3001 tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca ccggtatctc
     3061 agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga tttttaggca
     3121 tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc aaaagccgtt
     3181 tagtcgcgta gctaaaaaat ttctatcgat atttaaacga gaaaattgac gaaaagtatc
     3241 tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga tgagtcatcc
     3301 aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta acggtgaaag
     3361 tcgaacctgt tccaagatga ctgtctacag taatcgtacc attaaggagt tgcacgtagt
     3421 cttttacaat cgctaaccct aatccggtcc cgcctgaatg tcggcttcgc gccttgtcta
     3481 cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct ataccaaagt
     3541 cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc tcgctgtcgc
     3601 tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa aggatatctg
     3661 ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt ttcttttcga
     3721 tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa attgtctgga
     3781 tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc agtttatcta
     3841 accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc gggtcatctt
     3901 ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga gtttttaatt
     3961 cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc actttcgtta
     4021 agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag tagcgcaaac
     4081 tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc agcggatgct
     4141 caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact tctgtgtacc
     4201 gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga ttaagcagta
     4261 gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg ttcagtaacg
     4321 tatataattg ctcttctgtg gtagtacacg cccgatacat cttactcatt tgttcgctca
     4381 acgcattaat cgtctgatac aattcttccc attgatcggg tgtttgcatg attacttccg
     4441 cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc aatggctggt
     4501 ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa gacagaaaga
     4561 agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaacgctt gtcggttctg
     4621 cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg acatataaaa
     4681 gatcctcatt caaagtagta cttttgcgca aagcgctacc taggttccca ccattcaata
     4741 cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg tttgtgtcaa
     4801 ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga tggacgaatt
     4861 cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat tgccggatca
     4921 gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc tgttgaaaat
     4981 aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata agaaagaaga
     5041 gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat agccaaatcc
     5101 tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt ctcgcaaatg
     5161 actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc aaatgcggtc
     5221 tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa caagtaattc
     5281 aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat aattggtcaa
     5341 atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt ttgattgctt
     5401 acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc gaggactaaa
     5461 tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat ccacttggtc
     5521 atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca gccgctgagt
     5581 gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat caaatgattg
     5641 ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct tgtagccttc
     5701 tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa caactagtat
     5761 ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta gcatagaatc
     5821 agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca ctttgctgaa
     5881 tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc tggcctgtcg
     5941 ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa ctggagaagc
     6001 tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc ccgctgaaat
     6061 cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga cgacaagcgg
     6121 cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa aattgcagga
     6181 aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa cccattcgat
     6241 ttatgacgga agtttttccg gaataaaaaa aaatggtgcc ggagtggcta ctgcttattt
     6301 tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta gaggtgagca
     6361 gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat tcgaccttat
     6421 tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt tcattaggtg
     6481 agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc aagcggacaa
     6541 gcaagatctt ggaggattat gaaaaaaaac tccatctttt ttctaattat attgttcgag
     6601 aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa gataaaaaac
     6661 tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga atgaagaaaa
     6721 tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa taagaaaaaa
     6781 aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc ttgccttact
     6841 agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac gcgaacgtgc
     6901 ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa aaatcgttga
     6961 tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat caccaaaaat
     7021 accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct ttactggtaa
     7081 attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg caggggacgt
     7141 atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag acgttcctgt
     7201 atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga tggagttagc
     7261 aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc atatcgatga
     7321 aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg agattttgct
     7381 tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct tcaaccagca
     7441 attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag gcggggcagc
     7501 actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg gtgaaaaatt
     7561 gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg gtatggggga
     7621 tatgctgaca ttgattgaaa aagcgcagca agactacgac gagaaaaaag cggaagaact
     7681 tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac agcttgatca
     7741 agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa tgaatcaagt
     7801 ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa aagcgatggt
     7861 ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa gccgtcgtcg
     7921 ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga tcaaacaatt
     7981 caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca tcccaggtat
     8041 ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga tgaatcgaat
     8101 gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa acagcttcgg
     8161 tacagactga aaatgtctac tgccggagct tttctatcag caatttttat tctttactaa
     8221 tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg aacaaacaga
     8281 aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg accaaaagct
     8341 gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga cgattttaaa
     8401 taaaagtgaa gcattcgatg aagcatatga tcgattcgat atcaaaaaag tcgcaaatta
     8461 tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta accgaagaaa
     8521 aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag aaatcggcag
     8581 tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt cttatcattc
     8641 acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag atctaaagaa
     8701 aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag ctgtcgggat
     8761 catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta tcggcagaaa
     8821 gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag atggctattt
     8881 ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta agcaaactac
     8941 ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa cttatcagga
     9001 agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt tcgttttttc
     9061 cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc ctgttgcttt
     9121 tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg gcggtcgatt
     9181 gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga ttacccctgt
     9241 attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc gttttgaaat
     9301 gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa agtagaagta
     9361 aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc cttttttatt
     9421 accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca tctaaaatcg
     9481 tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt agattcgcaa
     9541 cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag acaagtgatt
     9601 ggcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca gtaacgacta
     9661 aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca tctaaagcat
     9721 ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg acaccgtcta
     9781 gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct ggagtttcga
     9841 gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg tacccttcag
     9901 gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt cttaaactac
     9961 caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact atctataata
    10021 atcttacaaa agataagtaa ctggagcaaa taaaaacact tactgacaaa agctacatag
    10081 aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt tctaaaaaaa
    10141 atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa aactgttaca
    10201 ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta aaatccgttt
    10261 aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg attcacgttc
    10321 tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga aagaccctgc
    10381 agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg cacaaccttc
    10441 tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc acgaagctaa
    10501 attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt cttaacaatc
    10561 gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga gtcgaaagac
    10621 ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat tggaaaacaa
    10681 gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa tgcaccgaaa
    10741 aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct gtactcgaat
    10801 tttcgagtgc agcttttttg ttttttttat taattgaaga aatgattatt tggcaaaaca
    10861 agcaagaaag cgtatcctta taggaggagg gagtgaacag ccaatgagaa agaaaatgtt
    10921 atttattcca tttgctctat tgttaagcgc ttgtagttcg gttactcaag aatcagacaa
    10981 tacacatcta tcaagcagta tatctgaaac tgttaccagt ccagaaaaaa gtagtaccaa
    11041 aacggcttcc accactgaac aaacaatggc tacctcaaat aatgagaaaa agactgcttt
    11101 agaccagttg aaagaacaac agccaaatgt tcccatgcct ctagatgtac cagtctcttc
    11161 aggctatctc aatatcgcag cgacacatac aaagcaaggt tattctattt tatattacag
    11221 aacagatcgt cccctcgggt tgaatgctga cgaattgaat caagaaacgc ctattgcaac
    11281 ctatctatat caatatgggt ttgcttctag tcaagaaaca atccaagttt tgcagccatt
    11341 tgagatcgac acaaatggtc agcaagtgga cttaggttct cgaatcacag ggtatcagca
    11401 aggtgcagca ggttcaagtt ttcttgaatg gcaagaagga aactggcgta ttcgaatccg
    11461 tgggaataat atagaaggcc aagatccctt actattagct aaagaaatcg tagcttattt
    11521 ggaagaaaac agtctaccag cacctgaaca atttggtaaa attactgttg atatgggtga
    11581 tacgacgaat cgagccgtag aagtaagctg gcaagaaccg aaaaatgcct atacgatcac
    11641 tcatcaagat cctatgtctg cgttgaagat ggcagtttct atgaagaggt tgtgaaatca
    11701 gcgcgctgat ctgagcccat catttaactc caagtcacag gaagtaatca aaaatagcac
    11761 gccgcatttt tgcataattt tggcttaata ccttaaaaga aaactagaat tgcctcttca
    11821 cacatgctaa aataaaacag acaaaaaaga aatggggata taagttgact gaatacttga
    11881 atgtaggaaa aatcgtaaac acacaaggta tcaaaggcga agtccgtgtt atctcaacaa
    11941 cggatttccc agaagaacgt tacaaaaaag gaacggtgct aacactattc caagacggaa
    12001 aagcacctgt ggaattaaca gtcaaaagtc atcgcaaaca taaaaatttt gacttattga
    12061 gttttgaagg acatccttct atcaatgatg tggaaaaata tcgggatggc atattaaaag
    12121 tgtcaaaaga caactctgta gatctagctg aaaatgaatt ctattaccat gagatcatcg
    12181 gtctgcatgt attagaagat ggaactgagt taggtaaagt aaaagaaatc ttgtcaccag
    12241 gtgccaatga tgtttgggtc gttcaacgtc cgaaaaaagc tgatatattg cttccatata
    12301 tcgcttctgt cgtcaaagaa gtcgatttag aagctggagt ggtccgtgta gaaattccag
    12361 aaggactgat tgacgatgaa gattgatgta ttgactttat ttccaagaat gtttgaaggt
    12421 cctatggggg aatccatcat cggtaaggct gtagataaag gattattaga tatcaatatc
    12481 tctaacttcc gcgattattc agataacaaa catcagacag tagatgatta tccatacggc
    12541 ggtggagcag gtatgctgct caaagtccag cccatctatg ataacatcca agccattgaa
    12601 aaagctgcac cggatgtcaa aaaaagagta attcttcttg acccagctgg taaacgattc
    12661 gatcagaaaa tggcagaaga attttcacaa gaagaacact tagtattcat ttgtggtcat
    12721 tatgagggat atgatgaacg tatccgcaca ttagtgacag atgaagtttc cttaggagat
    12781 tatgtcctta ctgggggaga attaggtgcg atggtcatga tcgatgcaac agttcggctt
    12841 ttgccagatg tgttagggaa tcagacttca gctcaaacgg attcttattc aaccggttta
    12901 ttggaacatc cccaatatac tcgcccggca gaatataaag ggatgaaagt accagaagta
    12961 ttgacaaatg gaaatcataa attgattgaa gaatggcaat taaaagaatc actaagaagg
    13021 acctatcaac gtcggcctga tcttttggaa acacttgaat ggacacctca aatgacgaaa
    13081 tttttagaag aaatcaagaa agaagaacaa gaaaaaacag cagatgaagc caagtgactt
    13141 tttctaaaat cttctttaca gtaggttgat aatatgttag tattttatgg tgagtgcaaa
    13201 agcacttaac tattacgata ttccgctgtg agaaaagctc ataagaatat ttggaataag
    13261 gagaagaaaa ccatgaatcc attaatcgaa gaattaacaa aagaacaatt acgttctgac
    13321 atcccagcgt tccgccctgg tgacactgtg cgtgttcacg caaaagttgt tgaaggtact
    13381 cgtgaacgta tccagttatt tgaaggtgtt gttatcaaac gccgcggtgc tggaatcagc
    13441 gaaacttata cagtacgtaa agtttctaac ggagtgggcg ttgaacgtac attcccattg
    13501 catacaccac gtgttgcaaa aatcgaagta gttcgctacg gtaaagtacg tcgtgcaaaa
    13561 ttgtactacc tacgtgcatt acacggaaaa gctgctcgta tcaaagaaat ccgtcgttaa
    13621 tcattgttat cagactgaga aagaaccctt ggtattcaag ggttcttttt tcttagcatg
    13681 taatttgaac accctgatcc agttttttct ttaattattg gtaggggaga ataatggatt
    13741 agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat atctttctta
    13801 agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt attcttagaa
    13861 tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaagatg aaagttttag
    13921 acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa aagaaataca
    13981 tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca gaaacagagg
    14041 ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa agacgttatc
    14101 taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag atgatgggaa
    14161 ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg cgatattggt
    14221 tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa gccaagaacg
    14281 ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt taaataagca
    14341 atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg cgttacatga
    14401 ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc ctaaaattag
    14461 attttggaca gatgcccttt aaaaagataa caagatttcg ttcttgaaac aatatatatg
    14521 cttttattcc aatgaggttg tgaaaacagt attttgtcct gagcgttaga aaattgaaaa
    14581 tggggaatag caactcgtac tttaccattt tttaagtgaa tacaagatca gctaattgaa
    14641 caccgtttat tcgtgatgag ggacacaaca ctgcagtcga cacatttctc ttttatgttc
    14701 aaagataaca aatatttaaa acaaagaaaa atagtagata atcccgtcct aactctttta
    14761 ttcttccc
//
LOCUS       DAZUMV010000063        14640 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000063 DAZUMV010000000
VERSION     DAZUMV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.063"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2101
                     /locus_tag="ACG8FK_001676"
     CDS             <1..2101
                     /locus_tag="ACG8FK_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS5097283.1"
                     /translation="KINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDE
                     NEGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPE
                     NQLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATK
                     IVVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLS
                     QPFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKL
                     VLTKENQIAGNYSAEIT"
     gene            2196..2693
                     /locus_tag="ACG8FK_001677"
     CDS             2196..2693
                     /locus_tag="ACG8FK_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFS5097284.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            2712..3176
                     /locus_tag="ACG8FK_001678"
                     /pseudo
     CDS             2712..3176
                     /locus_tag="ACG8FK_001678"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            3173..3931
                     /locus_tag="ACG8FK_001679"
     CDS             3173..3931
                     /locus_tag="ACG8FK_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5097285.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(4017..4757)
                     /locus_tag="ACG8FK_001680"
     CDS             complement(4017..4757)
                     /locus_tag="ACG8FK_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5097286.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            4818..6071
                     /locus_tag="ACG8FK_001681"
     CDS             4818..6071
                     /locus_tag="ACG8FK_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5097287.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            6338..6619
                     /locus_tag="ACG8FK_001682"
     CDS             6338..6619
                     /locus_tag="ACG8FK_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5097288.1"
                     /translation="MSLILGVEKQANPYDHKKMLTQQLRELEQDESIHRKAYNQVPPK
                     VEYSLTEEGKSLREILVALSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            6743..7069
                     /locus_tag="ACG8FK_001683"
     CDS             6743..7069
                     /locus_tag="ACG8FK_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5097289.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            7137..7850
                     /locus_tag="ACG8FK_001684"
     CDS             7137..7850
                     /locus_tag="ACG8FK_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS5097290.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            8160..9011
                     /gene="licT"
                     /locus_tag="ACG8FK_001685"
     CDS             8160..9011
                     /gene="licT"
                     /locus_tag="ACG8FK_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5097291.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            9132..10997
                     /locus_tag="ACG8FK_001686"
     CDS             9132..10997
                     /locus_tag="ACG8FK_001686"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5097292.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            11011..12453
                     /locus_tag="ACG8FK_001687"
     CDS             11011..12453
                     /locus_tag="ACG8FK_001687"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5097293.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            12561..13262
                     /locus_tag="ACG8FK_001688"
     CDS             12561..13262
                     /locus_tag="ACG8FK_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5097294.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            13401..13913
                     /locus_tag="ACG8FK_001689"
                     /pseudo
     CDS             13401..13913
                     /locus_tag="ACG8FK_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13924..14430
                     /locus_tag="ACG8FK_001690"
     CDS             13924..14430
                     /locus_tag="ACG8FK_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFS5097295.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(14427..>14640)
                     /locus_tag="ACG8FK_001691"
     CDS             complement(14427..>14640)
                     /locus_tag="ACG8FK_001691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097296.1"
                     /translation="YRRNWDFVLHVNNTHYLNSHTLVSPINEKSILFSCYVSPTPDVS
                     LNKKADEPNNRNLIILVHQLVPKPLY"
BASE COUNT         4908 a         2478 c         3149 g         4105 t
ORIGIN      
        1 aaagatcaat attaccaatg acaacaatcg cggaatcatc caaatgagag gttcaggatc
       61 gaaagtcact gtatcgaatg gtgggaaaat caccatcaat acagcaacta cgggcggatt
      121 tcgcttgcaa ggagattcta gtcaaatcca tgtatcaagt ggtggacaaa tctcggtcaa
      181 aatggctgga gatgacgatc aacccgggaa caatgggatg cgttttgtag gaaaaggcct
      241 ttctttgatc gtagaaggag ccggatcagt tatcgacatc gataagcaga gtgggcgttc
      301 cagcgcagtt cgttttgaga atggtacgca aaagctgaca gtaaccaatg gaggctcatt
      361 gagggtaaga aatacaggtg atggcaaatc ttacgtgaac agactcaatc aaggaaatca
      421 agcgatccaa ttttatgacg cttctggttt tctagaaagt ccaggttcag ctgattttac
      481 agtcactgga gaaaaatcaa atatcgatat acgcgcagat tccggagcaa ctgtagatac
      541 aactggagat attgatttaa attttacggc aggtgaaaaa acttatttag tcatggtcgg
      601 gaaaacaggg gaagataaga ccggcatcat cagtggggat gtgttgaatg tggacttaga
      661 atctcctacc tattttgatt tccaaaacaa acggattgga agcgagacca atggcatagg
      721 cggctgggta ttccaaggaa atggagattc ttctttaaag atcgcccatt cagaaatcgc
      781 tttatggcga aaaacaggcg caacattcga taactttgaa aaagacccgg attatttttc
      841 tgatatgaca gacttatcga tcaatgggac cgacttaggc aatttcgtat caagccctag
      901 tgcaggttta acaaatgaat ttgctcagac aggtgccgga atgaaaaatt ataatcgaat
      961 cagtgccaat aatcagctcc cgatgattga atccttaaga gtgccaacga atgcagataa
     1021 aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag gcaaagccaa gagatgcttg
     1081 gacaggcgaa gtagaagtga ctttacgtat tacttacgca gatacgacga aatcatctgt
     1141 agaaattact gctctcacgc aaggacaaac agatgaaaat gaaggggcag gatataaccc
     1201 atggggagaa ggcgaacaag gcggcttgtt cgaagttcaa gtacccaatg atgagagatt
     1261 aaaaacggag gatcaagtgc aagttgttgc tgctaaaaaa gtaagaggtg gccaagaagt
     1321 gacagaactg gactctgcca aaacgacagt cgatgtgatt cctcctgaac ctgctaattt
     1381 aagtaccaca ttgatcaatg cagcttctcg cacgatcaca ggtacaggtg aagcaggagc
     1441 cttggtttca ttgaaaaaaa atcaagctgt tttgtcagaa acaactattg atgatacagg
     1501 ggaatttgtt ctttctatcc cagaaaatca actttttgca ggagatgtat tagaaattgt
     1561 attaaacgac caagcaggag aagctactgc taagggtgtg cagaacaagc caagtaccaa
     1621 cgatgaaatt gggaatcaca atccaagtga tacgccagtt gtttatcatg atgccccgcc
     1681 ttttgcacct gctacgaaga tcgtagtaga aggtggtctc tcatttcttg ccccttccaa
     1741 actcgatttt ggtcagatca aagcaactgg tttgacacaa gaagcatttg gccaaatgga
     1801 tatacaggaa aaattatgga tttatgatca gcgagaaacg aaaaatccgt ggatcttaac
     1861 gataaaatta tctcagcctt tcacaaatga tgcaggattt tctatgcagg atgtcctata
     1921 tttcaagaaa ggagaggagt atcaattagt gaatgaagaa gaactggaaa ttgtcagagg
     1981 acaaaattca acgaatgtgc aagcagatta ttcagaatat ttaaataacg gtcgggcgtt
     2041 taaacttgtc ttaaccaaag aaaatcagat agctggtaat tactcagcag agataacata
     2101 gacattagca gatgcgccga cagaataaag atcaaggagg atgattgatg aatcgaatat
     2161 ggaataaaag tcatcgaaac aactgctctt tacttatgat ccttctcctt ggattcatcc
     2221 tttggcagac acctgtccat gcctccgaat tcaattttgc ggtagatttt gaattaccgg
     2281 ctaatcagat tgataagaac aaaacatata taaatgtaag gatggagcct gggaaaacac
     2341 aggaaatccc ctacacggtc tttaaccgaa cagataaatc agtaacagtt gagacattga
     2401 tccactccgc tacgacgaat tcaaacggtg tgatcgaata tggagaatca aaaagtaaat
     2461 ttgatgaaac attaagttat gatatgaaaa acttagcaaa aacagtggga gaaataactg
     2521 ttcctcctca aagtgcggtc actgagacac tttcactgaa agcgccagaa aaagagctgg
     2581 ctggtgtggt cgcaggaggg atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa
     2641 gtacagttgg tgtcgtcaat cagttttctc aagtgaaagc aatcttgatt tgaaatagcg
     2701 aaaaagagtc ttccccagat ttgaaattaa aaaaagctta tgcggatcaa agcgatggaa
     2761 gaaatgtgat taaggcgaat cttcaaaatc caaatgcttc ttatatgttc ggcttgacta
     2821 catcggttga aatctacaaa aaagaaacaa aagagcctta cctttcaata aaaaaagaaa
     2881 aaatagatgt agcacccaat acaaattttg atttacttgt cccattagaa ggaaaaaaat
     2941 tagctccggg acattatcaa gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc
     3001 tccataccga ttttgaaata actgatgatg cagcggacca attgaatgaa aaggatattg
     3061 atttaaaaaa tcagccagaa acatttgact ggcgatggct gatacttgtt ggagtcattc
     3121 ttattggctt atttgctgga ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa
     3181 aacaattgca ttaagtaccg ttctagctgg aagtctatta ttatgtacac aaagtgtttt
     3241 tgctgagaat aagagctcag ccactacaga aggaacgatt tctgtgacca aaccagatgc
     3301 tgcaaatcct gaagacgttg ttccagttcc tcctgttgat ccaaatgatc ctgatactcc
     3361 ttttacacct gacgaaccaa atcagggaac atccggatta ctgacgattg accaagctcc
     3421 agatcttgat tttggaacaa tcaagttagg tacagcaaaa acagtatatg ctgcattaca
     3481 aagcggaacg gacagtactg gagcacaaac acaagtacag aatttcgtac aagtaacgga
     3541 taaaagcgga aattatgccg gttggaaatt atcagtcaaa cgaacagaat ttattggtac
     3601 aacatctgat acgaataaac taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg
     3661 aaatagcgca actaatagca gtggaatgcc atctaccgtt tctggaactg gcgctgaagg
     3721 aatcgaaatc ccagcagatc aggcagtaga tctagtaact gcaggagcaa atgaagggat
     3781 tggcacatgg atttattcat taggagagaa tgtggaacaa gggagacaat cagtggaatt
     3841 atttgttcct gtatccaatt atgctgtaga caactacgtt tcaacattca catggtcgtt
     3901 aacagatgct cctacagaag aggaagcgta aaatcatatc aacgacttca gggagtggag
     3961 ccgattcaag gcccactccc tgaaatctgc tatgcttacg tctcagctct tattcatcaa
     4021 ctcgaagcag ttttttggaa gtcatggaaa cgttcctttg tcttttcaaa gatctttcga
     4081 ccaacgagta aggagtcacc gttgaaaaat tctaacacat aatgagcaga atcaattttc
     4141 tgcaattgcg ataagttgat acagtaagat tttaaaagta aaaagtaagc atacttttct
     4201 aactctgtct tcaattgttt taaagaagta ttcactaatt tatcttctgt agttgttttg
     4261 accataactt ggtttttcaa acctttgata ctttcaatat ataaaatttc ccgaggatac
     4321 aggacgagac ttccaccttt ttgatcaata aagattttat cgacatttac gatctgctgt
     4381 tctttccaat tgatctgttc tagcacttga cgaatttgtt cagataaatt cctgtctttt
     4441 ctgatatatc caatcggaag gattttccga ttgatgatct ctactgcaaa attcgtataa
     4501 gcggtgaaaa aaacaatatt actatgtgca ttttttttac gtatttgttc ggcgacatca
     4561 atacctgtga gtgattgctt caagtcgata tcgataaaga aaacatcgtg attatcaata
     4621 gtgcccaact ggatcaactg attgatttga gagatagaag tgagctgcac gatattctcg
     4681 atatgatgat tgaccaattc tctgatgatc cattcgctgt gaaactggtt atcttctatg
     4741 ataaaatact ttttcatatg caggctccta ttaaaaaaat atgtgtacat tttgaaacaa
     4801 aacaatggag gaataagatg gtcgcaacag tgacacttta tttgaccttt actttgattt
     4861 tagatttagg tatctattta ttcttttcga aacaactgct agtaaaaaac agctttggtt
     4921 gtatagcttt gatggtggtg acacttttac ttcctatccc taaagtgagc tttccttacc
     4981 taattggctt attattgatg tttctttttt attttactac gaaaaacctg tttatcagct
     5041 gtttgatcag taatcttttt tttgctatca ctggactcag cttttttctg gtttttgatc
     5101 tgatggatta tttaggaaag agacatcttg gaacagcaag tttttttgca ctacttatcg
     5161 catttatttt ctatctatta gtaaaaagta tagacaaaca gacgagattt ctcaacttgc
     5221 tttttactta tccaactgaa cactattttg gaggtatttg tattttatta agctggggcg
     5281 tgatttactt ggtattggga agtgttcact tccgctcatc taattatctg cttctatctc
     5341 ttttgtcgat agtcatcagt tttttttctt tgtttttttc cattatgttg atcagtttcc
     5401 aagtaaaaga aagacaacat caggaatcgc ttcaactgta tcaaataaat gaagaatatt
     5461 accatcatct agaggaattc aaacatgatt ataaaagtct actgttttca ttaaaaatta
     5521 ctcttcagga aaataaaaaa gaaacgttgg aatttttaga aatattagaa gatcattctg
     5581 cagctatttt tgaaaaaccg caaatacaac agctaacaaa tattgtatcc ccagctgtca
     5641 ggggtgtctt tttaaaattt ctagaaaaag cagaatcgga gaaaatcccg atccaagtag
     5701 ttattcctgt cgaagtgaaa acaatcccga ttgatctggt tgtttttatt cgctgtttat
     5761 ctatttttat ttctaacgca ttcgatcatc gtgtagccga tcagagtccg attacgatca
     5821 atctgctgga agaaaaagag ggatcacgtt ttagtatagc gaacaaggca aatccaacga
     5881 atttgacgct atcggagatg gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag
     5941 gtctatacag tgcgaaaaaa atgttggatg tttacgaaaa tgcagaacta aaaatcgaat
     6001 tttcaaaaca gaatcactat ttttttgtag aaatgtatct ggcatctaga aagagcaaat
     6061 cagcctactg acttactgtc aaaggataag tttgaggaaa gtctacacgg agatagagac
     6121 aacaacacag catattcaaa agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc
     6181 ttttgctatg tagttaaaca aagtagttga cacttccgct acttagtatt agtacgaaag
     6241 tatacttcat gatactaagt agataagaag tgaaaagcgg aacccacgat cgtcaaaatg
     6301 ctaactgctt tgcttacacc agataaaggg aagagcaata tcgcttattt taggcgtgga
     6361 gaagcaagca aatccctacg atcacaaaaa aatgctgacc cagcagttaa gagagttaga
     6421 acaagatgaa agtatccatc gaaaagccta taaccaagtc ccgcctaaag tagagtactc
     6481 gctcaccgaa gaaggaaaat cattaagaga gattctagta gcgttgtctg tttgggaaga
     6541 agaacgtatc gaaaaacaaa aagcctcaag ggaagaagac acgctactgc atacacatgg
     6601 tggctaccta aattattgat tttctatgac atttatttta aataaaagaa aacaggagtt
     6661 tttttaaaag catcatctat ttttttatgt aaaaaaatgg tatgattaat caagtaagaa
     6721 gtgattggaa gaaggcaagc aaatgaatag tatggaaaaa gtcaaccagt ttagagatga
     6781 acgtaattgg cgacaatttc ataacgaaaa agatttggct atctctattt ctttggaagc
     6841 atctgaattg ttagaacttt ttcaatggaa aacgccggaa gaagtaaaag aaacacaatt
     6901 agaacgaatc aaagaagaac tggcagatgt attgatctat tcatacatga tggcagacaa
     6961 ccttcacctg gatctagatg agatcattga agaaaaattg atcaaaaata atttgaaata
     7021 tcctgtagaa aaaagcgcag gtgtacgaaa aaaatacaca gagttgtaga aatgtgtcat
     7081 tcggctgttg gaaagagcaa aagaaaaaca gtaaaatgag tctggaggga gagaaaatga
     7141 aaaaaaaagg aaaagcaaaa agatggctag tcaatatcct cttgttcctc ttgttattag
     7201 taggactagc tttgattttc aatgaacaga tcaaggatta ttttgtcaga gagacaggag
     7261 ataaatatgc tatagcgaat gtaacaaaag aagatctgaa aaagaataac gataaagatg
     7321 tcagttttga ctttgatgcg gtagaaccaa tgacgacaga aggggtcatg aggtcgcaaa
     7381 tgagaggaac agatctacca gtgattgcta gtatcgcagt cccttcagtt tcaatcaatt
     7441 taccggtttt caaaggcttg gataatacgt cactacttta cggagcagga acattatctc
     7501 cagatcaaga aatgggaaaa ggaaattatg ccttagcaag ccatcgtgca acgaaccctg
     7561 aattgctgtt tacaccgcta gaaaatttag agatgggtgc caagatctat ttgacagatt
     7621 tagaaaatgt gtatacatac aaaacatttt ttaaagaaaa agttgctcct acagataccc
     7681 aattactgaa tgaagtcgag ggaaaagaaa ttgtcacact aattacttgt ggcgacatgg
     7741 acgccgtcac gcgactagtt gtacaaggag aactggaaag tgttacatcg ataaaagatg
     7801 cgacagatga tatgagatct gctttcaatc tagagacaaa aacattttag gatactggga
     7861 cataagtcgt tacagattaa aaacagccaa aggatatgaa tcgataagga aaaaccaatt
     7921 tgctgaaaaa aagagaaacg caaagaggtg catctctgat ttggttgttt tctagataga
     7981 cttgtgttcc agttttttta ttttttatat gaaaaggctt gcataaataa aaattcgatg
     8041 gtatgatgat tttgaataag aaatggattg ttactgtacg gcaggcaaaa ccagaattca
     8101 gctaagaaac agtaggcgaa ttctaggttt ttttattttt cttgagaggg cgaagcaaaa
     8161 tgaaaattga taaaattttg aataacaatg tcgtcatttc gaaaaatgga ttcggggaag
     8221 aagtcgtttg tatgggaaaa ggactagctt ttcaaaaaaa gattggggac gagatttcgc
     8281 cagaagcggt ccaaaaagaa tttgttttaa gagattcttt tgctaaaaac caattccagc
     8341 agctgatggc tgatattccg gcagaggaga tcgaatgggt caaacaagta gtcgaactgg
     8401 ctgaagataa actaaaagtt gaattttcgc ctaatattta tctgacattg accgatcatc
     8461 ttcattatgc gatcacacga gcaaaagaaa atatccaact gcctaatcca ttattatttg
     8521 aaacaaaaaa attttatccg aaagaatatc aagcagcgaa agaggctctt ttgttcgtga
     8581 aacaaaagac gggtgtggaa ctttctgagg atgaagccgg ttttattgcc tttcattttg
     8641 tgaatagcca acaaggaaac gaagacatgc aagtgacgat gaccgctacc acaatggtaa
     8701 gagatatact gagtatcatc agtaaattct tcggcatcgt gttcgatgaa gaatctttga
     8761 attatcagcg tatcattact catctgcagt tttttgccca gcgttatctc aaaggggaag
     8821 gatctgacga gcaagatgaa tttctctacg cattagttca aggaaagtat ccaaaagcgt
     8881 ttcattgtgt cgaacggatc aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga
     8941 tcgcagagca gatttattta acgattcata ttcaaagagt agtaagtgag aaaaaaagca
     9001 tccaatcata aggattgtta ctgtatggca ggcaaaacct ggattgaaaa aataggttca
     9061 cgaatgtttg tgactgtttt tttcaatctg ggtttttatt ttttaaataa aaaagaaaga
     9121 aggaaaaaac aatggataat caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac
     9181 aaaagaatgt caaaagttta gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg
     9241 aatctttagc agatacgcaa aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa
     9301 gtggcggtca gtatcaagta gtgattggca gtaatgtagc agatgtttat caatcgatcg
     9361 tggatgagga aggtcttgca gtttcggagg atggaaaaga agagtccaaa aatattttga
     9421 atcgtctgat cgatattatc tctagtatat ttacaccttt tctaggggcg atggcagccg
     9481 ccggggtatt gaaaggattc ttatcattgg caacggtgat gggctggctg acagctgata
     9541 gcggtactta tcaaatattg tttgctgcag cagatggtgt ctttaccttc ttacctgtca
     9601 tgcttgcttt tacagcagct aaaaagttca aagccaacca gtttttagca gtagcaattg
     9661 ctatggcact tgtgtatcca gctatcacag cagttgctgg tgcgggagaa gctatcagtt
     9721 tctttgggat cccagtgatt ctttcaccat ctggttatac ttcttctgtc ataccaatca
     9781 ttctagccgt atgggttcaa agtaaattag agccattcgt aaaaaaagtg attccgcaat
     9841 ttctgcaaat gattttagtt ccgcttgtcg ttttagtagt gatggttcca ttaacatttc
     9901 ttgctttagg accaatcggg acggttgccg gtaatgcttt aggcggtctc ttcaactcga
     9961 tttatggatt tagtccaatc gtagcaggtc taatcatggg aagtttatgg caagtattcg
    10021 tgatgttcgg catgcattgg ggattcgtac cgatcatgtt tttgaatatc gaacaatatg
    10081 ggtttgatgt attgatgcca atgcttcttc cagccatctt ggcacaaggt ggggctgctt
    10141 tagctgtagc attgcgaaca aaagatacga aacttcgagc attagggatt tcttctacag
    10201 tgacttcgtt atttggaatc actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga
    10261 aaccattcat tgccgcttgt atttctggag gtattggtgg agctattatc ggattttccg
    10321 gagtaaaagc tttctccagc agtttggtca gcttattgac gattccgact ttcattaata
    10381 cggtggatgg tgtagagtca aatgtgacag tagcagtaat agctacagga attgcttttg
    10441 ttcttgcatt cgtaggtaca ttgattttag gatttgacga acaaacacaa gacaatcaat
    10501 tagaaaacaa acatgcgaat gcaggagaac cgattacttc agcacggcat accttaaaaa
    10561 gtcctttaac gggtaaagta ctgccattga gtgaagtacc agaccaagtt ttttcttctg
    10621 gcgtgatggg aaaaggtatc gctattgatc cagaagtagg agaactagtt gcacctgcag
    10681 acggagagat cacgactatt tttccaactg gacatgctgt agggatcaca acgactgacg
    10741 gagcggagat tttgattcat atcgggatgg atacagtaga attaaatggg aatggctttg
    10801 agatcttagt caaacaaggg gatctcgtca aagcaggaga tttattgatt cgttttgaca
    10861 tcgaagcaat tagagcagct ggctatagcg taatcactcc agttgtcatt acgaatactg
    10921 atgcatttgc cgatatactt gaattagacc aaaaagagat tattgcaaac gaagatgttc
    10981 tggcaattgt aaaataagga ggaaatcagg atgcaatcac gtttttcaga aaaattttta
    11041 tggggtggag caactgctgc caatcaatta gaaggggctt atgatcgaga cggaaaaggg
    11101 ctatccgttg cagatgctat gcctggaggt aagcagcgct ttgcgattat cggcagtgaa
    11161 gaatttgact ggacgattga tcaagaaaaa tatatttacc cgaatcatcg aggaattgat
    11221 cattacgatc gtttcaaaga agatcttgcc ttgtttgcgg aaatgggatt caaatgttat
    11281 cgtttttcga ttgcctggac acgaattttt ccaaatggag acgaaggtac tcctaacgaa
    11341 gcgggattag aattttatga tcagctgatt gatgagtgct taaaatatga tattgaacct
    11401 gtgatcacga tttctcatta cgaaatgcct cttcatttgg caaaagaata cggtggctgg
    11461 aaaaatcgaa aactgatcga cttctatgaa cgatttgctc agacagtatt agagagatac
    11521 agcagtaaag tcaaatattg gatgactttc aatgaaatca attcggcctt tcactttcca
    11581 gctttaagtc aaggattagt aaaaagcaat ggagcaggag agtaccaaaa tattttccaa
    11641 gcatggcata atcaattcgt tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt
    11701 tcggatatcc aaatcggctg catgattatc tatgcaacaa cttacagcat cgatgccaat
    11761 ccagtcaatc aggcagcaac aatgatccaa aaccaagaat tcaatttttt ctgcacggat
    11821 gtccaagtac gtggagaata ccctgcatac acagcgcgta cccataaaaa atatggagtc
    11881 gatccggaaa aattagagca aacggaaaaa gacttccagt tgctaaaaga atatccagtg
    11941 gactacatcg gatttagtta ttatatgtct acggcaatca atgagactga tccacaagca
    12001 gcaacttcag aagggaactt gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa
    12061 tggggctggc agatcgatcc agaagggctg agaatcgcat tgaatgaatt atacaatcgt
    12121 taccagaaac cattattcat tgttgaaaac ggtcttggag ccattgatca tatggaagag
    12181 gatggcacga ttattgatga ttatcggatc gattatttac gtcgtcatat cgaagcgatg
    12241 gctaatgcag tagcagacgg tgtagatttg atgggctata cgccttgggg atgtatggat
    12301 cttgtcagtg catcaacagg agaaatgagt aaacgttacg gctttatcta tgtggatcta
    12361 gacgatgaag gaaatgggac actgaatcga tcaaagaaaa aatcttttga ttggtataaa
    12421 gaagtaattg cctcaaatgg cgtaaacttg taattaaagc agctgaatga aaaagaacac
    12481 acttctcaca tgtgatttgt gttctttttt tattttttat gtgctattct tttagtaata
    12541 gtaaagcaaa gggtgacgtt atgaaaagga cacgtgtact atacttggaa gtcgcagata
    12601 aaattaaaga agatattttc tcaggaaaat atccagtcgg ttcgatgtta ccgacagaaa
    12661 gtgaattaga agaactgttt caagtcagca aaatcactgt ccgaaaggca atcgaactgc
    12721 tggcatcaga tgaatatgtc gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc
    12781 gtccttacaa tcgattatcc aaagcggcaa gctttacgca aatcttggag aactcccgat
    12841 atgaagtccg gaaagaaacg ctggatttgc aattggtcca tataaagaag gaagatcctt
    12901 tgttttcctt tttcggtgag caagctgttt gttttaagcg cttatattat ttgaacaatc
    12961 agccttatat ttattttgtt cattatcttc cacaagaaat gatgcaattc agcagagaag
    13021 aatttggcaa agaatctttg tatcgtttgc tgacgaaaaa aggatatact attgactcat
    13081 tttctgatga tttctcggca gtatttttaa cagaagaaga acaagaaatt ctgaaaacag
    13141 acgcacagat cggcatcaaa cggatacgga aatcgtctac gcaaattggg gaaatgattg
    13201 aatattcgga ggcggtctac catacggagc tgcatcctta ccatattgat tatgaaacat
    13261 aaataaaaaa agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
    13321 aaaatagctt ctcatattag cttctcatat ttttcgtttg gttaggctta ttaccgcaga
    13381 tacccggctg tttctggatt accatttatt ctggataagg gggtgtggca cgattttcag
    13441 aaggaatcta tgaaccaatc actgtattga ttttagcttc gatggtaacc ccattcggct
    13501 tgacgatcgc ttatttctta gggaaaatca ttcgtaaaaa tatattcaat cgtcaagaaa
    13561 tcgatacatt gaagaccgct tttcctatgg ggatctgtca gatcacagag ggatgtatcc
    13621 ctatcgtatt gaacgacttg gcacgcaatg tcatagcaac gggagtcggt ggggcagtcg
    13681 gcggtggatt gagcatgttt tggggcgcag atagccgtat tccggcatcg ggaatgtttg
    13741 cagtcgctac gatgaccaga ccatgggcat tcattggtgc attgttagct ggttcttttg
    13801 ctacgggaat aactatttta ttattgaaaa aaccagttga cccaaatgca gaagtcatcc
    13861 aagaagaaaa agaggaagaa gagatatctt gggatgactt gacaatcagt taggaggaac
    13921 agaatggatt acaagaaaag agtacaacaa atgttcctag attcaggagt agtattgaaa
    13981 gaaacagaat tagaaagaat cgaatataca gattttggat taggagcaat cgagactgaa
    14041 ggattgaatt tatttgttta tgaaaataat gagcgatact gtgggaaaga actggtatta
    14101 ttgccttatc aaacttgtcc agagcatcgg cacccacctc gagatgagaa tgaaggaaaa
    14161 aaggaaacct ttcgttgtcg aaaaggctta gtatatcttt atgtcgaagg aaaagaaaca
    14221 aaaaaacttt cctgtattat tccaaagggg caagaacgtt actatacggc aaaaaaagaa
    14281 atcatacttc acgctggaga tcagtacacg atcgaacaaa atattaaaca ctggttccag
    14341 gcaggtgcaa caggcgcggt tatcaccgaa ttttcttcca gcagtgatga tccaagtgat
    14401 agttttacga atccagctat ttatcgttaa tacaaaggtt tgggaacaag ctgatgaacc
    14461 aaaatgatca aatttcgatt atttggttcg tcggcttttt tatttagaga aacatcaggc
    14521 gttggtgaaa cataacacga gaataaaatg gatttttcat taataggaga aacaagtgtg
    14581 tgactattta aataatgcgt gttattgaca tgcaggacga aatcccaatt tcttctataa
//
LOCUS       DAZUMV010000064        14638 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000064 DAZUMV010000000
VERSION     DAZUMV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.064"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(185..751)
                     /locus_tag="ACG8FK_001692"
     CDS             complement(185..751)
                     /locus_tag="ACG8FK_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5097297.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(751..1707)
                     /locus_tag="ACG8FK_001693"
     CDS             complement(751..1707)
                     /locus_tag="ACG8FK_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS5097298.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1707..2363)
                     /locus_tag="ACG8FK_001694"
     CDS             complement(1707..2363)
                     /locus_tag="ACG8FK_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5097299.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2733..4751
                     /locus_tag="ACG8FK_001695"
     CDS             2733..4751
                     /locus_tag="ACG8FK_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5097300.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4821..5027
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5178..7592
                     /gene="leuS"
                     /locus_tag="ACG8FK_001696"
     CDS             5178..7592
                     /gene="leuS"
                     /locus_tag="ACG8FK_001696"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS5097301.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7940..9493
                     /locus_tag="ACG8FK_001697"
     CDS             7940..9493
                     /locus_tag="ACG8FK_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS5097302.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9874..11658)
                     /locus_tag="ACG8FK_001698"
     CDS             complement(9874..11658)
                     /locus_tag="ACG8FK_001698"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097303.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11673..13394)
                     /locus_tag="ACG8FK_001699"
     CDS             complement(11673..13394)
                     /locus_tag="ACG8FK_001699"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097304.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13494..14255
                     /locus_tag="ACG8FK_001700"
     CDS             13494..14255
                     /locus_tag="ACG8FK_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5097305.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4754 a         2706 c         2913 g         4265 t
ORIGIN      
        1 cctgcatcaa gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgatttggtt
       61 ggacttatta ccgcagatat aagcttttga gcaccttgaa taaagaggtt atgaaatcaa
      121 cgctctaacc tgagtattag agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt
      181 ttatttatct ttttttctca atacaatata aaataggcgg ctggttcttt tggttgataa
      241 accgataatt cagcacatta tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca
      301 tatccagttc tttttctcca ccttcatgac catagtagac gacaaggatc aatcgtccgc
      361 gaggcacaag tcgtttcaat atttcttcca tagctgtgcg tgtagtctct ggcatagtga
      421 tgattgcttt atcacttttt gggagatacc ctagattgaa aatccctgct ttgagatttt
      481 gttgttcagc tatcacttct cctaacgttt cgtggccttg tagaaacaat gagacacgtt
      541 cttctaactg acgctgttct agcctttcac gagtagcatc gattgcttgt tgctggatat
      601 caaaactata gacatgacca gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc
      661 cattgcccat cgttgcatct acgacgtgat cccctggttg caagatttct tgcagtaatg
      721 tatggctgaa atgcaaagca gtttgcaaca tcagacgctc acctttttcc gtacatcata
      781 ttttccttgg aagctatctc tgcgaagaag ttcttcatca attgcattca agacttccca
      841 ttttttcaag ctccacattg gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg
      901 atggatgatg atctcttttg gtatcatttc cagctggtcg caaatgacat tgacatagtc
      961 aggacggctc attaattgaa ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg
     1021 catcagatgc atcaaatgga gcttgattcc ttggatatct gaatctaaaa tggtgcgtct
     1081 gacattttct ttcatcattt ctaaactttc gcctggtaat ccgttgatca aatgcgtaca
     1141 aacacggata ttatgtttgc gtaatttagc cacgccatct aaataagttt gataatcatg
     1201 tgctcggttg atcagttttg aagtttcttc aaaagttgtt tgcaatccaa gttctaccca
     1261 taaatacatc cgctcgttca gttcagcaag gtaatcaact acttcatctg gcagacaatc
     1321 cggtcgtgta ccgactgata atcctacgac acctggtaaa ttgactactt gctcaaatcg
     1381 ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta
     1441 ttgttggaca tgaggccatt tttgatgcat catgtcgatt tctttctgga attggattgg
     1501 aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca gatacactgc aaaaagtaca
     1561 accgccatgg gcaacagtcc catctcgatt cggacagtca aatcctccat cgatcggcac
     1621 cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa ttccaagaat gatatctttt
     1681 atttccttct gaataaggaa aagtcattaa aagtccttct ttctgtaaga gttatctatt
     1741 tgttttcttt tttctctata cactggataa gtcaggcaca gccaagaaag actgagtaag
     1801 tagccgccga tgatatcagt aggaaaatga acgcccagat aaatgcggct tatcccgaca
     1861 cttactataa taaacatcat cagcaattga agcaatgtgc gtaaggttct tttttgaaca
     1921 aaagctggaa gcagaaatag taaagaaccg taaagaatca tactgcctgc tgcatgtcca
     1981 ctagggaaac tgtaacttgt ttcaactact aagtgcgcca atgaaggacg ctctcgcatg
     2041 aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta caataaatcc agaaacgaac
     2101 caaaatgttt ctgccctttt tttcttatac cacatccata aagccgcaca agcaaataaa
     2161 atgatgacgg taagaggatt accgaattta gtcataaaga gttggtagct gttcagatca
     2221 ggataagaag ctctgacaat gcctgtaaac cactgatcga aaccagaaag ccacgacaga
     2281 tggaacttta cgatccagcc caaaatgaaa aacaggacaa gaaagaagct accagccacc
     2341 tgaaatgatg ttttatattt catacaattc ctcctttgaa gcaatgactt attatagcat
     2401 ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa tgtatgattc ctctttgaat
     2461 aaccaatatt cttataaaaa aaataactgt aatcggaaag acattttttg gtaatgcaaa
     2521 tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac
     2581 gtaggaagaa cttgaaaatg aacagaagtt gttcattacg gtaaattaca atatgattac
     2641 aaaaaacaac aaagctctta aataactttt aataaatatt aagagaagtt ataattatta
     2701 ataatagata aattgaagtt ggaggataga agatggaaaa cattgcaaga aaagaacgga
     2761 gacgtttaga acagaccaaa cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa
     2821 ctgcgatggt aggctgttct gtcgctgccc cattagtaca gccggttcct gttcaagcgg
     2881 atgaaacacc tgttcagttc ggcgcaagaa tcaatacagc ggcatttatc acagaaattg
     2941 ctacttatgc tcaaccaatc gctcaagcca acgatctata tgcatcagtg atgatcgcac
     3001 aagcagttgt agaaagcggt tggggcagta gtgcgttatc tcaagcacct tattataatc
     3061 tttttgggat aaaaggcagc tatcaaggac aaaccgtcta catggatacg cttgaatttt
     3121 taaataatca atgggtaaat aaaaaggagc ctttccgaca atatccttct tttgctgaat
     3181 ctttcaatga caatgcctat gtacttagaa atacttcatt tggcaacggt tattactatg
     3241 ccggtacttg gaagagcaac acgaaatcct atacagatgc gacagcttgt ttgacagggc
     3301 gttatgcgac tgatcctggt tacgcaggca aattaaataa tatcatcgca acttacggat
     3361 tgacccaata tgatacacca gctagcggaa acgcaggcgg tggagcgact acaggtggca
     3421 ataatactgg aagtgcaggt agtactggcg gttcaagcaa ttctggtaat acaaactcag
     3481 gttcagcaac ttcgaccact tatactgtcc aatctggtga ttctgtttgg ggaatctcaa
     3541 ataaattcgg tatcacaatg gatcaattga tccaatggaa caacatccaa aacaatttca
     3601 tttatccagg acaaaaactg acaatcaaag gtagccagga aaatggctct tcgacgaata
     3661 attccggtaa caataccaac tcttcaggaa atgctggtac atcaaacgga ggacaaacaa
     3721 ctggtgcgaa atacactgtt caatctggtg attctgtttg gaagatctca aatgatcacg
     3781 gaattacgat ggctcaattg attgaatgga acaatatcaa gaacaatttc gtttatccag
     3841 gacaacagct aatcgtatca aatggcggtt cttctaatgc tggagcaaat actggctcaa
     3901 atactgggaa tacaagtggc tctagttcgt ccagctcaag ttcttctaat acagcttcag
     3961 gaacgaaata tacagttaaa gccggagact ctgtttggag cgtctcaaat aaatacggaa
     4021 ttacgatggc tcagctaatc caatggaaca atatccaaaa taactttatc tacccaggac
     4081 aacaactgac tgtttcaaac agtggatcaa caaacagcac gtcaggtaat acaggttcgt
     4141 caaccaatac aaataacagc agttcagtta atactggcag cagttcgtct caaggcactt
     4201 ataccgtgaa agcaggtgaa tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg
     4261 atcagttaat cgaatggaat aatatcaaaa acaacttcat ttatccagga cagcaattga
     4321 tcgttaaagg tggaacttcg gcaaatacaa atacaggttc aactacttct gcagcgaaac
     4381 caaatacacc gaatacgtct gctacgacaa gtacatcatc cactggtaat acgatgtata
     4441 ccgtgaaagc gggcgagtca gtttggagcg tagcgaacaa acatcatatc acgatggatc
     4501 agttaatcga atggaataat atcaaaaata atttcatcta tccaggacag caagtgattg
     4561 tgaagaaagg ttcatctcaa tcaacaaacc aacaatcagt tactggttcg aaaacctata
     4621 ctgtgaaatc tggagaatct gtttggagtg ttgctgacag tcatggaatt acaatggctc
     4681 aactaatcga atggaacaat attaagaaca actttattta tccaggtcaa acgttgattg
     4741 tagctaaata aaatagctgt tcgaagaaat aacgattgaa ttccacaaaa atatttgtta
     4801 ctataaggct acttaaatat gacaacactt gaaaaggaag tagtaaaaag actgaagttt
     4861 cagagagcgc atggctggtg agaatgtgca cggatgcttt tgaactcgcc tttgagttgc
     4921 ccttgctgaa tacccgagta agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt
     4981 ttcactaagg taaaattagg tggtaccgcg tagattacgt cctataaaaa gagcaatctt
     5041 tttataggat ttttttattt cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa
     5101 aataaacaga ttctgccggt tttttcgtta tatttataat agaaacaagt gttttcaata
     5161 gaacaggagg acagcaaatg agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat
     5221 actgggcaaa aacgaatact ttcaacaccc atgatgatcc tgaaaagcca aaattttacg
     5281 cactggatat gtttccctat ccatccggtc aagggctaca tgtagggcat cctgaaggtt
     5341 atacggcaac ggatatcctt tcacgtgtga aaagaagtca aggatacaac gtgctgcatc
     5401 caatggggtg ggatgcattc ggtctgcctg ctgaacaata tgcgctagat acaggaaacg
     5461 atccagctga gtttacccaa aaaaatatcg aaacattccg acgccaaatc aattctttag
     5521 gatttagtta tgactggaat cgcgaagtga atacaactga tccggagtat tataaatgga
     5581 cacaatggat ctttacgaag ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg
     5641 taaactgggt acctgaatta ggaacagtta tttcaaatga agaagtgatc gatgggaaaa
     5701 gtgaacgtgg tggctatgat gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca
     5761 ctgcttatgc tgatcgctta ttggatgatc tagaattagt tgattggcca gaaagcatta
     5821 aagaaatgca aagaaattgg atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg
     5881 ctggtacgga taaatcctat accgtattta cgacacgacc agatactcta ttcggtgcaa
     5941 cttactcggt tcttgcacct gaactagatc ttgttagaga aattacgacc cctgaacaaa
     6001 aagctgctgt agaagcttat atcgaagaga cagctaaaaa atcagatcta aagagaacgg
     6061 atcttgcaaa agaaaaaaca ggagtattta ctggtgctta tgcaatcaat ccagttaatg
     6121 gcaaggaaat cccaatctgg atcgccgatt atgtattatc ttcatacggc acaggagcca
     6181 tcatggctgt tccagcacat gatgaacgtg actttgaatt cgctcaaact ttcggactag
     6241 aaattcttcc agttatcgaa ggtggagatg ttgaaaaagc tgcttacaca gaagatggta
     6301 cgcacatcaa ctctgaattt ctagacggca tgaacaaaca agaagcaatc gataaaatga
     6361 atgaatggct agaagaaaat ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact
     6421 ggctattctc ccgtcaacgt tactggggtg aaccaatccc aatcatccat tgggaggacg
     6481 gaacagtcac agctgtccca gaagaagagc taccattacg gttgcctaag acaaaaaaca
     6541 tcaagccaag cggtactgga gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg
     6601 ttgatcctgt aacaggtaaa aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag
     6661 gaagctcatg gtattacctg cgttatatcg accctcataa caaaaaagaa ctagcagatt
     6721 atgaaaagtt gaagcgttgg ctaccagttg acatctacat tggtggagca gaacatgcgg
     6781 tacttcattt actatatgca cgattctggc ataaattctt atacgatatc ggtgttgttc
     6841 ctacaaaaga accattccaa aaactataca atcaaggaat gatcttaggc gaaaacaacg
     6901 aaaaaatgtc aaaatctcgt ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg
     6961 gtgcagatac actgcgcatg tacgaaatgt tcatggggcc attagatgct tcgattgctt
     7021 ggagcgaaaa tggcttagaa ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg
     7081 ttgatgagaa caataaaatg cgtgaccgta tcactacatt gaatgatggc aagcttgata
     7141 aagtgtatca tcaaacagtg aaaaaagtga cagaagacta tgaaaatctt catttcaata
     7201 cagcaatctc ccaattgatg gtctttatca atgaagccta taaagtggac gctctgcctt
     7261 atgaatatat cgaaggattt gttcaactac tcgcaccgat tgctcctcac atgggtgaag
     7321 aactatgggc aatccttgga aatgatggtg gtatttctta cgcaccatgg ccaacatacg
     7381 atgaagcagc acttgttgaa gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc
     7441 gtgcgaaaag caatgttccc cgagacttag gaaaagatga gctagaaaaa gcagcattag
     7501 ccaatgaaat agtgcaagaa tatattgaag gcaaaacagt cagaaaagtt attgcggtac
     7561 caaacaagtt agtcaatatc gttgctaatt aattggctct ttgtcaataa ggactgatga
     7621 gttgtgcaaa atcaaatctg ggccagaatg aacttcattc tgtctcagat tttttgttat
     7681 ttcaaattga ttaattgatt gatcaaaaag gttttgattt tcatgttctc aaacacgttc
     7741 aatgtctttt tcttctgaat ggtcttgtaa gagatctctg taatcatcaa atttttgtct
     7801 attaatctta gtattttttg atgtataaac tacctgaaat tgagagtaca taccaaattc
     7861 ataaattttt tattagtggg actagtgatt gtatggattt tgacgcataa tgaatgaagt
     7921 atgagaaaag gagagacaaa tggatcaaga actatttatt atcgaagtat tatcagctaa
     7981 acaaaaaatt atcgagaaag cgaaaaaaag agcgaaaaga ataatgttca agcttttaag
     8041 cttagtattt ttctctggtt tgcttttttt cggagctgta tcgttcctat atcgccatat
     8101 gagtgcagta gtccgttcag ctgaagggat gcaaaaatta aataatttgt atcacttttc
     8161 gtggggagtt ggcggaataa caattgttgt gcttattgtt tttcttctac ttatttggct
     8221 ccgttaccat caattgtcgc gtcatttcga tcaatggctt agtcaaagag cacaacagtt
     8281 tttacataat aaaccaactt ctcaagatga tgactatctt tattttgaga agacttcgac
     8341 gagtcaagct tttcggatac aaaaaagtga ttgccagtta ttgctgagat cacttgatga
     8401 tttacctatc tacttaggcg aaagcaaatc aaaattcgga atgaacacat tccatatttt
     8461 tttactagaa aacgaaccca gatgggaaaa aagccagtct tcgcctggtg gatacctgac
     8521 aactaagatg agtacattag tcactggttt tttcctcatt cttttagctg gaacaatcta
     8581 ttatgggaga tacactcagc caaaaatgtc ttctcatata aatgctgctt ctcacatgac
     8641 aatgaactca actaacgata cgcatataca aaagacaaat gaagggttgt tagctgatcc
     8701 taagcaaaag gtaaatttga caacacaaaa gataaatgat ctcaaacttg atacagagac
     8761 gcactctctc tatatgacaa cagatagtgg tgctaattgg gtatttgtgc cgattgattc
     8821 gcaatggttg cgttttggcg aatacaccct gacaacgggg ctgattcctg aaggttattg
     8881 gatggacaac acatatgata tttccgccga tttctcatgg tttatctatt cttctgggga
     8941 tgatgtacgt ctgctgactt caagagataa tggtaaaaca tggcaaacgt ctattatcac
     9001 ccaatcagtt cagccgatac gttatcgaaa agcggtattt tatggcaacg atggtctatt
     9061 tatctgtaca tcagccagtg ggatgtctgc agaaactttg tacatttaca ctactatgga
     9121 tggcggaatc acttggataa aaagtggaag tacaatgatc aatcagccag tacaaaatgt
     9181 cagcttcgtt acaacttctt taggttttgt ttcgacacgt gaaagtttgt tttatacaaa
     9241 caacggagga cgctcattca aagaatcagt tctgtcgatt ccggaaaact atcagacagg
     9301 ggggatagat ctttttcaat caccaaatga agttactcaa ataagcgcga atacgttaga
     9361 gacaaaattt tatttgttga aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct
     9421 cttccgttca actgataacg gggaaacatg gcaattcgtt cagcaactat cgcaagtaga
     9481 acaaggagat taaagaagag agattgtgag cctgacgttc agcaccgagt cacaagaaac
     9541 aaattcaaaa aacagcttct catatttgtt gaatagtgta actcgtgatt cggagttgac
     9601 aagagcaaca agaagcgatt ttgaagtata gcttctcgta ttttacaaga tcgtgtctta
     9661 ttgccgatag gttgtctctt gaacaccgtt tattcgtaaa aagggattgt gacaagtctt
     9721 ttgtcactac ttaccgaacg aaactgaagg agctatccaa aaagtatcat tagtattatt
     9781 ctttgtgttt ctagtggcac cactagtaca tttaaaaaag ctatcgatca tggactaatc
     9841 atacatgatc gatagctttt tagataatgt ttgttacgct atctgaaatt gactattata
     9901 aagactggca taaagcgttg gtgattggag tagttcagaa tgtgttcctt tttcgacgat
     9961 atcgccattc ttcattacaa ggatcatgtc tgcattttca atcgttgaca aacggtgtgc
    10021 aatcacaaaa cttgtcctat cttttgtcac agcttccatt gcttgctgaa tcaatgcttc
    10081 tgttcgtgta tctacgctag aagttgcttc atctaaaata acgacaggag gattcgctag
    10141 aatgactcga gcaattgtca ataattgttg ctgtccttga gataacgtgc cgttttcgct
    10201 agaaatgatc gtatcatatc cgtctggcag tgttcgaata aagtgatcac attgtgcgat
    10261 ttcagccgct tcgataattt gttctcttgt ggcatccatt ttcccgtagg cgatattatc
    10321 agctaccgtt ccttcaaaca gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag
    10381 ttctccacgt gaaagcttgg taatatcaag accgtcgaat tcgatctttc ctttattcag
    10441 ttcataaaaa cgcatcagca gattgactaa agtcgttttt cctgcaccag ttggaccaac
    10501 gatcgctacc gtttgttttg gctttactga aaaatcgact tgattcatca atattttatc
    10561 tgatgtgtaa ccaaactgta catttttgaa agctattttt cctttcggtt gttctaaaag
    10621 tcttgcatct ggaaggtctg gaatttcatc tgtttcatct aatatctcaa atatcctatc
    10681 gattgctgcc attgcggatt ggatcgagtt gatcacataa gaagcggtag aaattggttc
    10741 agaaatctga ttgatgtact gaagataagc ttgtaagaag ccgatcgtca caccacctga
    10801 tagaacaagc atagcaccga ataccgcact tatgatgaag gcgagttgat taatgaaacg
    10861 aatggccgga tagatagcac agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt
    10921 tttatttact tcgtgtatag cagcttctgc atgagcttgc tgattgaatg ttttgaccac
    10981 tagattacca gctaagattt cttccattcg attatttaaa acacccagtt catactgatt
    11041 ctgttcagaa atctgtttgt ttttgttcgc aatcttagtt gtaacaattg aactgccaac
    11101 gatcagcaat aagacgagca aagtaagctt gctttcgata taaaacagca tcagaccagc
    11161 agcagctatt gttacgacag aagtaaaaaa ttgattgata cctgtcaata acacttgaga
    11221 aagttgattg agaccggtag ttgtccgact aatgatgtca cctacttgat gattgtcaaa
    11281 aaacgacatc ggtaatgttt tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat
    11341 cttttcgctt aacgctgcca ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac
    11401 tgaacagccg atcagcaata agacagggaa caacaatgct tgtttgatat cactgaaaga
    11461 aagctgtcca atacctgttt ttcttatttc tgataagaga tcatcgatcc cgattcccat
    11521 gatcaagggc atggctacga ctaataggtt cccgaataaa ctgcatagaa acaatccgta
    11581 aaaatattta tgttcaggtt tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa
    11641 cgtttttttc ttttccatcg tatcccttct ttctatattc cttgtgattt tgcaaactcc
    11701 tgataagttt ggcaattaga cagtagttct tcatgagtac cttgtccaac gatttttcct
    11761 tcatctaaaa caatgatgtt atctgcttgt tggattgtac tgaggcgttg agcaacaatc
    11821 acgagagttt tctccgacat gtgtttatga agggctgctc ttaatgctgc atctgtttta
    11881 tagtctaaag cggagaaact atcatcgaat acatagatct ctgctggttt gatcaacgct
    11941 cgagcaatac atatccgctg tttctggccc cctgaataat tagaaccgcc ttgagcaaca
    12001 aagctgtcca atccgtcagg aagggtgtcc actagttctt ctaattgagc gatcttgatc
    12061 gctttagaca actcttctgt tgaggcatat tcatttccca tctgcaagtt gcttaaaatt
    12121 gtcccgctga ataagaaagc cttctgaggg acataactga tgtgggagcg gatctgttct
    12181 tgagtcattt gagtaagagg aataccacca agggttactt ttcctatcgt agggtcacta
    12241 agtctcaaga gaagtttcgc caaactactt tttccagatc cggtcccgcc aacgatcgct
    12301 gttgtttttc ctttaggtat tgtgaaatca atatcttcta gaactggatc agagatttca
    12361 gtatatttaa atgtgacatg ttcaaaagca gctgcatttg tctgatcaaa ggttggttca
    12421 gctactgttt tgatcgtctc ttcctcaatt tcgatctctg cttgcagtat ttcttctatc
    12481 cgtcgtaatg aggcaaacga tttagggata atgaccaata ccatagctgc gatcatcaag
    12541 taagcaagtg tcagcataga gtattcgact acagcagtca cgacaccgat ttgtaatgct
    12601 ccttctgcta caagatttcc gccaaaccac aaaatagaag cataaacgat tcccatgagc
    12661 aaaaaagcaa ttggagtgac ccaagcaaat attcggttca ctttgatcat ttgattagca
    12721 tagtcagcaa agctcttatt cgttcgcttt tcttcatatg attgattatc aaaggcacgg
    12781 atcatattaa tcccagtaaa gaattctctc aatgtgatgg tgatccgatc catttttggc
    12841 tggatactca aagataaagg tgcccctcgt ttcatcagtc gataaacaat ccatacataa
    12901 aaaacaacag agatcaatgt aacccatgtt agtaatagag aataacgaaa catcatgaga
    12961 atggtgaaaa cgcaaataac cggtgctggt aagatcattt ggcaaaataa aacaatcatt
    13021 tgctggacat tatcgacatc attcgtcatt cttgtcaaaa gagatccaga actaaaacga
    13081 ctgatatcct tcattgacag ctgttggaat ttacggaaat atttctcccg cgtttggaat
    13141 ccaaacctag cggaagccaa tgctgaaaag taactagagg caactgcagc gattgttcct
    13201 agaaacgcta caagcagcat ttcaactcca atttttttta taactgaggc ttcgccactt
    13261 gctatacctg tgtcgatcag ctttgctact aattttggta cacctaatgt accggcgatt
    13321 tctaaacaga tcatcacaaa ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt
    13381 tttaagatag acatattatt cctccttttt tcctgatact tttcgattct ataccttcaa
    13441 gtaacttgaa ggtcaataga aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa
    13501 aaatgtatac gattggccaa gtatcggaac tatttgattt gccaaaatca acgatccgct
    13561 attgggatga acaaggatta atccgttcaa gtagacaaga agagaatgat tatcgattgt
    13621 ttgatatcga tgatattttt atgctttatg atatcgtttt ttatagaaaa ttagatatac
    13681 cgatcaaaca gatgaagaat ttatatggaa aaacattgac ggagttatat gagacacttg
    13741 atgaaacaga aagaagaatc cataaagaac tggtggtgat gaaacaaaaa caaaaagaga
    13801 taagggagca aaaaaaacag ttgaaattga tgatcgatac aaacgaggaa gaatttcctg
    13861 tagaagagat cccttttgac tgtatgatct caacggagtt tgaagatatt gtggaaatca
    13921 aaaaatttct acctaactat tcaagctttg ggatgatgag tatgccagct tccagttcac
    13981 agacgatgta tggatttttt atcgacccta gtgaagtaca tttatttact caagacgtga
    14041 tttgggagaa aaaagatacg gctgtttatc gtcgattctt attgaaatcc gagatgaacc
    14101 atgcggaacg taataatatt ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa
    14161 caggagaagt gatcggccag tatctattga cgaatacgga tgaaaacaat atcagaactg
    14221 aatattatca tgcatggata gaaatgaaaa aataggaaat atgtgtttaa gataactcga
    14281 aaatgatggt tgttttagtt ataaagaaaa attttctggt tcgattacgg gtttaatgtg
    14341 ggggattatt agtcgtggat agatagaatt ttagtttttt agaggaaata tgtctttaaa
    14401 aatggaatgg ttgcaattct tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat
    14461 ggaaaaataa ttgtattggg tgttatgtat aattcatggt gaactgagac taatgagaag
    14521 aaaaattcat attgttatga gtgagattgt atcagatgag aatgataaat taaataggtg
    14581 cataaaagca ttacacttaa aatacattgt tgcttacatt attacaatat gttaataa
//
LOCUS       DAZUMV010000065        14309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000065 DAZUMV010000000
VERSION     DAZUMV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.065"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(324..2321)
                     /gene="tkt"
                     /locus_tag="ACG8FK_001701"
     CDS             complement(324..2321)
                     /gene="tkt"
                     /locus_tag="ACG8FK_001701"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS5097306.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2423..2674)
                     /locus_tag="ACG8FK_001702"
     CDS             complement(2423..2674)
                     /locus_tag="ACG8FK_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS5097307.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2821..3444
                     /gene="lexA"
                     /locus_tag="ACG8FK_001703"
     CDS             2821..3444
                     /gene="lexA"
                     /locus_tag="ACG8FK_001703"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS5097308.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3623..3841
                     /locus_tag="ACG8FK_001704"
     CDS             3623..3841
                     /locus_tag="ACG8FK_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097309.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4129..4641)
                     /locus_tag="ACG8FK_001705"
     CDS             complement(4129..4641)
                     /locus_tag="ACG8FK_001705"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS5097310.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4655..6952)
                     /gene="recJ"
                     /locus_tag="ACG8FK_001706"
     CDS             complement(4655..6952)
                     /gene="recJ"
                     /locus_tag="ACG8FK_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS5097311.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7094..7537)
                     /locus_tag="ACG8FK_001707"
     CDS             complement(7094..7537)
                     /locus_tag="ACG8FK_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS5097312.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7538..8326)
                     /locus_tag="ACG8FK_001708"
     CDS             complement(7538..8326)
                     /locus_tag="ACG8FK_001708"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5097313.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8323..9264)
                     /gene="rnz"
                     /locus_tag="ACG8FK_001709"
     CDS             complement(8323..9264)
                     /gene="rnz"
                     /locus_tag="ACG8FK_001709"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS5097314.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9441..10028)
                     /locus_tag="ACG8FK_001710"
     CDS             complement(9441..10028)
                     /locus_tag="ACG8FK_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS5097315.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10249..11562)
                     /gene="obgE"
                     /locus_tag="ACG8FK_001711"
     CDS             complement(10249..11562)
                     /gene="obgE"
                     /locus_tag="ACG8FK_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS5097316.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11850..12860
                     /gene="gap"
                     /locus_tag="ACG8FK_001712"
     CDS             11850..12860
                     /gene="gap"
                     /locus_tag="ACG8FK_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5097317.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12945..13880)
                     /locus_tag="ACG8FK_001713"
     CDS             complement(12945..13880)
                     /locus_tag="ACG8FK_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5097318.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14066..>14309
                     /locus_tag="ACG8FK_001714"
     CDS             14066..>14309
                     /locus_tag="ACG8FK_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5097319.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIK"
BASE COUNT         3998 a         3184 c         2512 g         4615 t
ORIGIN      
        1 agcaatcgct tttttattga tcaccatttt tcttttaggt ctatggtgtc gggcactgcg
       61 ggtcttcagc tcttgcattc aaattcctcc tcaaaataaa aaaacttaat aaaaagttct
      121 cttcttaaaa caaacttaat taattaaatt atacttttaa ttctcaagaa acacaaacta
      181 atccgaagaa ttctttgttt tgtcatttaa atgtaacata gccgaaaacg aaaagtaata
      241 ttgatttatt cttctaacac ataaagaaca gctgtctcca acttgcagac agctgttctt
      301 atcagtcaaa caattgcttg gatctatgat agttgattgt acgtattcac gacattttct
      361 actgtaaatc caaattcttc taaaatttta gtgccagggg cagatgcacc gaaatgatcg
      421 attgtaatcg ttgcgccctc tgttcctacg tagcgttccc aaccaaatga tgcagcagct
      481 tcgatagcca cacgtttctt cactgattta ggaagtacac tttctttata ttcagaagat
      541 tgtttttcaa agaggtcaaa gcttggcata gaaacaacag aaacatcttt gcctttttca
      601 gccaattctt tttgagcttt cacagccaaa tcaacttcag aacctgttgc aatcaaaata
      661 ccttctggtg tttctccttg tgatggagaa agaacatacg cgcctttttt caccatttca
      721 tctgctactt cttttgtaga aggcaatact ggaaggtttt gacgactcaa tacaaggata
      781 gtaggcgcat ctgttgtctc catcgctact ttccaagcag cacgtgtttc atttccgtct
      841 gccggacgaa tcacttgaac accaggcatg cagcgaacag atgctagttg ttcaatcggt
      901 tcgtgcgttg gtccgtcttc tccgacagca accgagtcat gtgttaagac gtaaacaaca
      961 ggagtatgtt ggattgctgc taaacgaact gctggtctta gataatcgac aaagacaaag
     1021 aatgttccac cgtagatacg agttccgcca tgaagttgga taccattcat agccgaagcc
     1081 attgcaaatt cgcggacacc gaaccagatg ttgcgtcctt cataatgttc aggagtgaaa
     1141 tctttatctg cagccaccat tgtattattg gaacctgaca agtccgctga accaccccag
     1201 aagcttggta tagctttcga taattcttgg atgacttctt tacttgaaac acggcttgct
     1261 tgactgcttc caacttcata agttggcaat tctgcatccc agttttctgg tagtttgcca
     1321 tcaaaagcat cttcaaattg ttgtgctaat tcaggataag cttttttata attggcaaac
     1381 atttcattcc atgcatcttc agctttttgt ccttcttcta tcatcgtttg atggaagcgg
     1441 gcagctactt cttctggaac agtgaaatct ggatactccc aaccataaac ttctttagcc
     1501 atcttgatgc catcttcacc aataggagca ccatgaacag cagatgttcc ttctttagga
     1561 gcaccatagc cgatgactgt ttttacttcg atcaatgttg gtttgtctgt ttcagctttt
     1621 gcttcttcaa tcgctttaga gattgcttct aggtcatttc cgtctttcac taagatatgt
     1681 tgccagccat atgcttcata gcgagcacca acgttttctg tgaaagcttt tgaagttgga
     1741 ccgtctagtg aaatatcgtt tgaatcgtat aaaacgatca atttccctag tttcatatga
     1801 cctgccatcg aacttgcttc ttgagatacc ccttccatca agtctccgtc accgcatatt
     1861 gcatacgtat agtgatccat cacattaaag ttttctttgt tatacgttgc tgcaaggtgc
     1921 gcttctgcca ttgccatacc gacagccatt gcaatccctt gtcctaatgg ccctgtagtg
     1981 gcttctacac catctgtgtg atgtacttca ggatgtcctg gtgttttaga atcccattga
     2041 cggaattgtt tcaaatcatc gattgtcact tgatagccag ctaaatgaag caagctatac
     2101 aacattgctg acccatgtcc tgccgaaaga acgaaacggt cgcggtcagc ccagtttttt
     2161 gatgtcgttg gatttacttt caggtgtttt gtccataacg catatgccat aggtgctgcg
     2221 cccatcggca atccaggatg tcctgagttt gctttttgga ctgcttcaat acttaatgta
     2281 cgaattgtgt taacgccaag ttggtcaatt ttgtcaaaca aaataacccc tcctattaat
     2341 ttaggttttt tttaccttta tttcttacac tttcattcta taagatgtca cacgaaaaag
     2401 caatcttttt cagtattttt tactaaaagt tgtcatttcg gttgtgtaag ccttttttct
     2461 tttggatttc ttttaatttt tccggggtca cgtcattgcc ctcaggatcg acgactttca
     2521 ttccttcgat atgatgacgc ataccggaac gaaaagcttc caaatactct ttgcgtaagt
     2581 tgatttgttc ttgttcttcg gcttttgaga gaccattttc cttcttttta cgtgcaagtt
     2641 cattgatcct ctctattttt tcaggtgaca gcatgctcag ccctcctttt actctccttc
     2701 atattatata aaaactgaaa aaaaaacaac tagataaact aaataaaata cgaacattcg
     2761 tttgatttag cgctttttat atggtactat ttatttatca agtgaaagaa ggtgtacctt
     2821 gtggcccgac aaactgaatc aagacaaatc gaagtcttac gatatatcca tgaacaagta
     2881 acccaaaaag gatacccgcc gactgtccgt gaaatcgggg aagctgttca gctttcttct
     2941 acttctactg ttcacggtca tcttgcacgg cttgaaaaaa aagggtttat ccaaagagat
     3001 ccgacgaaac ctcgtgcaat cgagttgaca aaacaaggtt tagaaaagat tgggattcgt
     3061 ccaacaacta ttccagtact tggcgtcgta acagctggag aacccatttt agcagtagag
     3121 gatgcttcag acttctttcc aatccctcct aatctttctt ccgaagaagg cagtctcttt
     3181 atgctgacaa ttcgtggaga tagtatgatc aatgctggga tcttggatgg tgacaacgtg
     3241 atcgttcgga agcaagaaac tgctcaaaat ggcgatattg tcattgcaat gactgccgag
     3301 gacgaagcga cttgcaaacg attctataaa gaaaaaaact ttttccgtct gcagcctgaa
     3361 aacgacttgc tcgaaccaat catcttagat caagtatcta tattaggacg agtagtagga
     3421 ctttatcgta gtcatatcta ttaattgcta ctaataaaag aaaacttttt aaaaagagtt
     3481 tgagacgatg gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca
     3541 cttccctctt tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca
     3601 taaattaacg aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt
     3661 tttgttttta ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt
     3721 tccgttattt actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg
     3781 gcgcagaatt catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata
     3841 ataaggattg actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac
     3901 aaaaatcggt cacatccttt tttcgaataa acggtgttct aaagccagtt tttcggtatt
     3961 aagtcaaatt gcggaaaaag tgatttttta aacttctact ggtctttgct tgtttccatt
     4021 tataaaaaaa tagcgaccct caaaagtcag atagagaatc tgacttttga gggtcgcttt
     4081 aaaatcgctt ctttaagaat aacatcaaga atagcttctt ttttctgctt aatattccat
     4141 taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca tggagatcca tcaattcgat
     4201 caaaaatgcg cagcctacga ctacaccgcc taattgttcg atcagttcga tcgtcgcttt
     4261 gattgttcct ccagtagcta gcaaatcgtc acaaatcagc acacgttgac ctggtttaat
     4321 ggcatcctta tgcaatgtca atgtatctgt tccatattct aatccataag tcacttcgat
     4381 ggtttctcga ggtaacttcc cttttttgcg gactggggca aaaccgactc ctaactcata
     4441 agcaacagga catcctacga tgaatccacg ggcttctggc ccaactacca tatcaattcg
     4501 tttttctttt gcataattta cgatttgctt cgtagcttcc cgataagcgt caccatctgc
     4561 catcagtggg gaaatatctc gaaacacaat tccttttgac ggatagtcgg gaatactggc
     4621 aatataatct tttaaatcca ctctttgttt cctcctaggt tgttaaccat ttctttatgg
     4681 taggaatatc gctcaaaagc aaaaattcct caactttgat tttattgagc cgccgctggt
     4741 ataatctgct ctcagtcaaa ggatgacttt ctgggctagc gactttttgt aaaacaccgt
     4801 tctctattgt aacaaatttc aattcaaaaa acacctgtat cataaaaatt aataattttt
     4861 cagggatacg caaatattga gctattgctt ttattttatg acgtatatca attcgttctt
     4921 gagaatggat cagtttaaac aattttgcgt actgttctct tgtgcctacc ccattcaaat
     4981 aggcctcatc aggcgaaatc cccatcagat aaacacgatt gaatgaataa gaccgaaaaa
     5041 cttctttcag atccgctaaa tcatctggac aatccataat aactagagag ttcggttttc
     5101 ttttttcaat tattgccttt acagaatcta gtccttcgaa aacaatcgca gcttcctgga
     5161 taatctcagg cacgaatttc aacgtagaag aatcaaatgc taaataaagg ctaccttccg
     5221 acgttcggct ctgttcacgg aaacgttttg cccgccaatc aaatacttgc agaccaccca
     5281 cagaaaaatc agataccatc agctgcggtt tcttacgccc gttccattca ttgatcgaaa
     5341 gctttcctac cacatctacc tgattattca gcagttcttc ttcttgagta ccaaaaccaa
     5401 aagcgaccgc atcaagctga ctcgatgcat tggaaaggac aaatttcaga tgctgttggt
     5461 ttgctccgat tcttttgata ttttctgctt ggacttgtcg aaataaaaac tgaggcaaag
     5521 gattatccgt accaaatggc gccaatagtt tcagctcatc aattcgctct actgttactt
     5581 catttggcag caatacttca tcaattctca actcaggccc tctcatcaag tcaatttgat
     5641 gatcgactat atattggttc atcttctcct gtagtatagt gacattttca cttggcatcg
     5701 ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa taaatctcgc atctggtcaa
     5761 gcatttcaaa aagattcaag gcttcaatac tacgtccaga tcctttagct gatccatctt
     5821 cttttaaagt aaggacaagc gttggtttac ccgtttcatt cattattttc ccagcaacaa
     5881 tgcccaaaac gccttcatgc catcctcggt ttaccaatag atggatatga ttctccttat
     5941 tgaccatcgc taatgcttct tgtgttatct gctctacgat tgcttttctt tcttcattga
     6001 tctcattcaa tttttttgct tgaacggatg cttcctcttc atcaaacgtc gcaagtaacg
     6061 agacggcagg acgtggatcg cccatccgac cgattgcatt tagtctggga gcaatcgaaa
     6121 aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc gctttcttca aatagcgcct
     6181 gaagtcctat ccgttctgaa tgatgaatgg cattgattcc taatgaaaca aggattcgat
     6241 tctcatcagt caatgaaact aaatcggcga tcgttcctat cgctactaaa tccaaaaatt
     6301 ctacaggcgg ttcttccaat aatgcagaag ctactttgaa tgctactccg actcctgcca
     6361 aatctggaaa tggatagtgc cccgctggat gacgagggtg aacaatcgca taagcgtctg
     6421 gtaaattctc aggtagttcg tgatgatctg taatgatcac gtctacacca gcttgctgag
     6481 cgtatgctac cgcatcattt cctgctaccc cgttatcaac agtgatgatc aactggattc
     6541 cttcttcgat cttttcttgg tatacagatt ggtttggtcc atacccatgc tcaaatcgat
     6601 ttggcaggaa tacttctact tctgcaccaa gtaattccag tgtttctttc attaccgttg
     6661 cactggtgat cccatctgcg tcataatcac cgtaaacgag gattttttct ccactgatta
     6721 cagcttcttg gattcggctg attgctttat ccatatcgtg catcagatac ggatcatgta
     6781 attcttgttc ctttggatgt aaaaatcggt tgatatcctt ttcctcatgg taacctcttt
     6841 gccacagtaa cttaccgaca aatggagata atttcagttc acggatcatt tggagaaagg
     6901 attcagaagg ctctgtctcg cttaacttcc aatcaaaatt tgcttgtctc actttctcac
     6961 tcctaactta gttattatag caaaggaaat cagcaaaaaa aagcagaaag aaagaataaa
     7021 aacagatcag atccgctaga cagatgttca gcaggacctg atcttttgta cgaccattta
     7081 tgaaatatcc ctattagtca aaataattga agctttgttt gggcgattga gttttttgtt
     7141 cttccggcgc tttcaattga ctgatttgtg cggttttcgc ttgtaactcc gcctgataag
     7201 cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg ctgttcttct tttgcttctt
     7261 caattttctt ttgaagttct gtttgatatt ctgacatttc ctgagtcagc tgtttgattt
     7321 gctttttttg ctgccatagt gttgccgaag cagtcaaaaa tacaatcagt gcaccaatca
     7381 gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa ggaagtgaag ccaaaactta
     7441 caggaacatt tttattgttc aatacagcaa aaagaacaat aatcaaaact aaaacaaatc
     7501 caagaatcac attgctttgc tttttcaatt ccttcaccta ctttttgtta aaaattccgc
     7561 tggctaaata atcaccaatc gttggaaaca gcgtataaaa ttttgatgca acttctaaaa
     7621 taaacggttg attgatttcc cgttttggat gtctcatatt gcggacgatc atttttgcca
     7681 acttggcagg ttctaaaacg atttgtccga ctttttccaa atagcttccg cttggatcag
     7741 ccttatcgaa gaaatctgtc tcgattggac ctggattcac tgtcgtaaca gctacgccaa
     7801 gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag gactgcaaat ttcgtggcag
     7861 aataaaccgt tgattttgca gttgccattt ttccagccat agacgcgata ttaatgatat
     7921 gaccttgtct tctttctgcc atatcgatcg caaatttttg tgtcaaaacc atcattccta
     7981 agatgttgac ttcaaacata tcgtatgctt ttccaaggtc gaatgtaagg aagtcttcga
     8041 aaatcccaaa acccgcattg tttaccagaa tatcgacgcg cccgacttct tccatcacct
     8101 tttcatataa ttcgtccacg ctttcaggat tgctgacgtc taccttgaaa gcataagcct
     8161 ctttgccgct caagcgtctg cactcgtctc tgacaccttc tacaaatgcc attcttcgtg
     8221 cgcaacttat aacgattgct ccttgctttg ctgcttcata acagatttgt gcgcccagac
     8281 cgcctgaact acctgttact aaaacaactt tatcttctaa attcatgcct cttcctcctc
     8341 gcgaaatgga atctcaatgc tatcaaaatc tttcatgatc gtcgtgtttt caaacacttc
     8401 ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa tatctcgcac tgatatgagt
     8461 aagtagcagc cgttttgcct gcgcttcttt tgcaacttca gcagcttgat gagtcgtgga
     8521 atggaaatag ttgtgcgcca ttcttgcttc atctttatta aatgtgcttt catgtaccag
     8581 aatatcgcta ttttccgcaa gcacaacgga attatgtgtt tttctcgtat cgcctaaaat
     8641 agtcacgatt cgtccttttt tatctggacc tacgaaatct tttccattga tcgtccgccc
     8701 atcttctagt tggatcgttt caccttgctt cagtttgccg tacagtggtc cagctggaat
     8761 acctaattct tttagacgat ccacctgcaa ttctccttta tgatcatgtt caaccacacg
     8821 ataaccaaaa ctgtcgatcc catgatttag tttttttgta taaacagaaa attgctgatc
     8881 agtaaaaatc ggggctgttt catttagctc aatgaatttc agcggataac ttaaacgcgt
     8941 ttgcgaaata cctaaagaga cttttatgta ttcctctatc ccttttggcc cgtaaatctc
     9001 taatggcgta tccccacctt gaaatgaccg gctgctgatg agcccgggaa gtccaaaaat
     9061 atgatcgccg tgtaaatgtg taataaaaat tttaccgatt tttctcggac gaatagtcgt
     9121 tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac aaccaaactt cattgcgctc
     9181 gtctaaaagt ttcaatgcta tacttgtcac atttctatgt tttgcaggaa cacctgcacc
     9241 tgttcctaaa aattgtaatt ccataatcta cctgactttc tttaagttcc tctttaattt
     9301 tagggtaaaa agaagggata atcaaccgta agtcgagaat gggttcaaat taaaaataac
     9361 gttctaaagc gatagcgttt tctttgaaaa ttactataaa aacagcagcg aacccgtttg
     9421 agatcctctg ctgtttttta ttaatttgaa gatccagaac tttcattgtc gttggaagtt
     9481 tgcggatgtt tgtccttcca agtcttttgt gcagcttctt tcgcatgatt ttcagcttct
     9541 ttcgcatcat gcacatcttt ttcgacatct tcataatcca agcgggtaat ttctccgcct
     9601 aattccatca ttaccaattg attcagtggg cgattgtcct cttcatcagc aatcaagatc
     9661 agtcctgtag ctccttcacc gattttattt cctacaaagt cgaacacggt ctgggcatct
     9721 tttatttctt tggcatcttg tgtcgcaccg atcattcctc cggcaaacca tcctaataaa
     9781 atccctagcg gaccacctaa tacaccaacc aacattccga ttaatccacc cgtagaagat
     9841 ttgttattac cggtaaagtc gataaaatct tctattttga attgatgtgt cccattagga
     9901 tcatggctga caactgccat ctgttctcct ttaatcgaac gaacagcatg taactttttg
     9961 atttctgaaa atgcctgata tgcctggctt tctacttgaa aatttaaaat gattactcgt
    10021 ttgctcatta aaaacgcccc tttcatacct tcttgttatt tattttacct ttaattgtaa
    10081 aataagaaaa gaaaagcact ctttttttcc aataaaatag ggctgtgaca aaagtcgtat
    10141 cacagctcct ttttccgaat aaacggcgtt cttgaagctg atcacttctg tcacaacctc
    10201 ccttaaatct gaatctatat tttttggtat ttgtttttat ttgcttgcct attcgacaaa
    10261 ttcaaattca aactcaccga tacgtacaag gtcgccatct ttcgcaccgc gtgcccgaag
    10321 agcttcatcc acacccatac cacgtaattg acgagcaaaa cgcatcactg tttcatcatg
    10381 atcgaaattc gtcatttgga ataatttttc gatcttctca ccagataaga tccacgttgc
    10441 atctgaatca cgatcgattt ggaactcagg accttctgga ttgaatccat agtgtacagt
    10501 ttcttcttcc agttcttctt cgtacaatgg gaattctggc gttacttcta acaaatctgc
    10561 tgtagcattc aataacgcgt ccaatccttg tcgagtcact ccagaaattg gaaatactgg
    10621 gatgtcatca gcaaattcat cttctttttc cttgttcagc tgttctttga atttcactag
    10681 attttccggt gcatccggca tatccatttt gtttgccacg atgatttgcg gacgctctag
    10741 caagcgtaag ttataagtag ataattcttt gttgatggca agatagtctt cataaggatc
    10801 tcgtccttcc atcccactca tatcgatcac atgtaaaatt actcgtgttc tttcgatatg
    10861 acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct ccttcaatca agcctggcaa
    10921 gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc attcctagat tagggacgag
    10981 cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat gaaataacgg atagtaaagt
    11041 cgatttcccg acagatggaa aacctacaag tccaacgtct gccaatactt tcaattctag
    11101 ttcgattttt cgctcttgtc ctggttcgcc gttttcagca atttccggtg caggattccg
    11161 aggagaggca aaacggatat tccctcgtcc accgcgtcca ccttttgcca caactagtgt
    11221 ttgaccattt tcaattaaat ctcctaaaag agcaccagtc tccgcatctc ggacagtggt
    11281 tccttgtggt actttaacat aggtatcttc tgaaccacga ccgtgcatcc ctttactcat
    11341 cccattttca cctggctgcg ctttgaaatg gcgattgaac cgaaaatcca tcaatgtacg
    11401 taacccttca tcgacgatca agatgacatc gccgccacga ccaccgtctc cgccagccgg
    11461 tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc attccgtctc cgcccttgcc
    11521 agctttcaca tcgatcgtta cttgatctaa aaacatggac ataattaagt cctcctatta
    11581 ttcatttatt tatttgactg cctgaatttc cacaataaac aaaagtctta cttattttat
    11641 cggtgaaatc tgattttgtc tatcatttca gcaaaaagat tgtttacctt cttctttttt
    11701 tatcatttgg taaggtttcc aacctttttt ctgctaccaa gtataatttt ttgacaaaat
    11761 attctttgcg tagcagaata acagtgtatt aattgtatta attgaggatg ccgtttcaca
    11821 gcacccctaa taaaggagga acttttgata tgacagtaaa agtaggtatt aacggatttg
    11881 ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt ctcagatgat attgaagtag
    11941 tagcaatcaa cgacttaaca agtccaacta tgttagcaca attattgcaa tttgactcta
    12001 ctcatggtac ttatcctggc acagttactg caacagaaga ctcaatcgta gtggatggcg
    12061 aagcaactcg tgtttatgca gaacccgacg caagcaaaat cccttgggca aaagaaaacg
    12121 gtgtagatat cgtactagaa tgtactggtt tttatacatc tgaagaaaaa gcaaaagcgc
    12181 atttagatgc tggtgtcaaa cgagtagtta tttctgcccc agctggtgct atgaaaacca
    12241 tcgtctataa cgtcaatgat gatacattag atgaaaacga caaaatcatc tcagcaggtt
    12301 catgtacaac aaactgttta gcacctatgg cttatttctt aaataatgaa tttgggatcg
    12361 aagttggtac aatgacaaca gttcatgcct acacttccac acaaatgctc ttagatggac
    12421 ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc tgacaatact atcccacatt
    12481 ctactggtgc tgcgaaagca atcggcttag ttatccctga gctacaagga aaattacaag
    12541 gacacgcaca acgtgttcca gtcgttgatg gttcattaac tgaattggtt tcagtattga
    12601 aaacaaaagt aactgctgat caagtaaacg aagcaatcaa aaaacacaca gttgacaatc
    12661 cttcattcgg gtatgatgac cgtcaaatcg tttctagcga tgtcattgga acaactcaag
    12721 gttcgatctt tgacccaacg caaacagaag taacaactgc tggtgatttc caacttgtta
    12781 aaactgttgc atggtatgac aatgagtatg gatttacttg ccaaatgatt cgcttactag
    12841 aaaaattcgc taacttgtaa tttgatagat caatcgataa aagaagcctt tgactggatc
    12901 cagtcaaagg cttctttctt ttcatcccct acttttaaac tcgttcaatg aacatattgg
    12961 cttaaaaatt gttggactac ttgctgatat ttttcaggat ctgtcttgta tgcttccgca
    13021 tgctcggctc cttttacaac atacttttct tttggtccct tcgttgcacg atagacctta
    13081 tctaacattt caaatggtac aaatgtatct tcgtctccat gtataaagag cattgggcgc
    13141 tggtttttct tcaattgttc cactgcattt gcttcaccga aaaagtagcc agcacggact
    13201 tttgtcacta gactcgttac ttgcataagc ggaaatgctg gaagactaaa catatcgtct
    13261 agctgataag caagctcatc atgaacggaa gaataaccgc aatcttcaat gattgctttc
    13321 acattatctg gtagtttttc tccgctagtc atcataactg ttgctgctcc catgctgaca
    13381 ccgtatagaa cgatttcttg cgattttcca ttgttttcta gcactttgtt tatccactgt
    13441 acatagtctt tacgctctgg ccatccaaat ccaatgtaat ccccttcgct ttttccatgt
    13501 cctcttgcat caggaacaag gacattgtaa cccatatcat gaaacatctt tgcgtaattg
    13561 gtcatcgttt cagcgtttcc catatagcca tgagccacaa gagctgtttt ttcacttttc
    13621 gtttcagcag gaagataaat cgctgataat ttcagaccgt cttttgaagt gatggtccaa
    13681 tcgcttcggt tttcttttga actaaaccat ttatgtgcat ccgtttcttt ggatgtctgt
    13741 gttgtaccag gtgtatcttc gtctaaaaag tctttctcag aaggcacaac agcataatta
    13801 taaaaataat tccctgcaaa cactaacgca acaatcagag ctaaagcgac gcctaaaagg
    13861 attttcatcc aaagtttcat aaatcatcca tctcatttct cttttttcat ataaccttga
    13921 attagtataa tcaaagaatc tctactatgc aatgttcatt tttataaccc gcgaattgta
    13981 aaattaagtt agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa
    14041 agaaaacaaa ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac
    14101 aatgatagaa tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg
    14161 acgtgctata caaactattt ataatgtagt caataagttc aagcaaggca agactgctct
    14221 tgattattgg caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt
    14281 acctgctcat gaagtagatt acattaaag
//
LOCUS       DAZUMV010000066        14245 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000066 DAZUMV010000000
VERSION     DAZUMV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.066"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(158..2236)
                     /locus_tag="ACG8FK_001715"
     CDS             complement(158..2236)
                     /locus_tag="ACG8FK_001715"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5097320.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            2729..3946
                     /locus_tag="ACG8FK_001716"
     CDS             2729..3946
                     /locus_tag="ACG8FK_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5097321.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(4405..5028)
                     /locus_tag="ACG8FK_001717"
     CDS             complement(4405..5028)
                     /locus_tag="ACG8FK_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097322.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(5184..6581)
                     /locus_tag="ACG8FK_001718"
     CDS             complement(5184..6581)
                     /locus_tag="ACG8FK_001718"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5097323.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(6603..7574)
                     /locus_tag="ACG8FK_001719"
     CDS             complement(6603..7574)
                     /locus_tag="ACG8FK_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5097324.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(7581..9881)
                     /locus_tag="ACG8FK_001720"
     CDS             complement(7581..9881)
                     /locus_tag="ACG8FK_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS5097325.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(9883..11208)
                     /locus_tag="ACG8FK_001721"
     CDS             complement(9883..11208)
                     /locus_tag="ACG8FK_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5097326.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            11439..11864
                     /locus_tag="ACG8FK_001722"
     CDS             11439..11864
                     /locus_tag="ACG8FK_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097327.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(12362..13063)
                     /locus_tag="ACG8FK_001723"
     CDS             complement(12362..13063)
                     /locus_tag="ACG8FK_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5097328.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(13079..13519)
                     /locus_tag="ACG8FK_001724"
     CDS             complement(13079..13519)
                     /locus_tag="ACG8FK_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5097329.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(13519..14094)
                     /locus_tag="ACG8FK_001725"
     CDS             complement(13519..14094)
                     /locus_tag="ACG8FK_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS5097330.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         4222 a         2990 c         2382 g         4651 t
ORIGIN      
        1 aatttaaatt ttccgcattc aataaataga tacgttaaaa ataaaaaata cacgaaaaaa
       61 ataaaaaaat gtttttctca taaaaaatac caaccttcaa gcatgagact gctgtctaat
      121 ccctgaaggt tggtttgatt gtttattgat cattttttta ctgctccgca ttggttcctg
      181 ttactggata taggatagtc gttgttttat tgccgttttt tgaaaagaca ctggtcgaat
      241 gttcaccttt ttgcttttcg attgcttcat tacgttccgc ttcgttttta tagtcatatt
      301 ttttcggatc aatcgtctca aatccgtttg gcacataaaa ccgcaagaga ttttcctgat
      361 tcagcttatc tgaaagactt aacgcagtct tgactttttc ctgatctttg gagatttcct
      421 cgtcgattcc tgctgctttc gtatcgatga tttctcctgt ctgattattg taagcttttc
      481 ctcctaggac ggtgtatttg ggcgtcacga agttcccatt tcgaaaagca actacttgat
      541 cgtgctgaga agataataag tctgtaccaa aatgaagata atctttatca tcgattccca
      601 atagatgtag taatgtcggc aaaacatcga tttctccacc atattcatga ttgactgtcc
      661 ctttagtgta accaggcata tggatcatca atggcactcg ttgcatctgt gcgttgtcaa
      721 attcatccca tgtatctgga tctttaccaa gagcacttgc taaatcttta ttatctgtat
      781 tcgagatccc aaaatgatcc ccgtaaatca caaagattga attttggtaa ataccagatt
      841 ttttcaaata cgtaaaaaat tgttccaatg attgatccag ataatgggcc gttcgaacat
      901 aattatctac tgtgctattg ccagtattca aagatggaaa gtcgaaattc ttgtcatccg
      961 tataataagg cgtgtggttg gttacggaaa gaaatttcgt gtaaaacggc tgttgtagat
     1021 gttcaagata ttgggcagat tctctgaata aatctttatc taatatccca taacctaacg
     1081 tttcatcgga ttcatcaaaa taagatcgat caaagaaatt gtcataccct aaatttttat
     1141 atacatgatc tctattccag aaactagcta cattcccatg gaaaacagca cttgtatagc
     1201 cttctgtttg accaagggtc gctggtgctg cttgaaagac attatctgac ccaagttctg
     1261 tgaataatga gccttgaggt aaaccaaatg tgccagtttc caacatgttt tcagcatcac
     1321 ttgtctttcc ctgtcctact tcatgaaaga aattgtcaaa actgatcgtt gcttgattct
     1381 ggaaaatact atttaaaaac ggcgtgactt cttgtccgtc taccttcata ttgatcaaaa
     1441 actgttgaaa actttctaaa tgaaggacaa tcacattttt tcctttagca atcccaaatg
     1501 tttcaggatt tggctctgca taatgtttct ttgtatagtc taaaacttca tcaattccat
     1561 tactgcttgc atgcgcgcga acactgctag tcattcctgt tttgattccg tcataaatag
     1621 tgtacgcatc gattcctaga tatttgacaa tataggaacg gtcaaatgtc cgctgtagca
     1681 attggggacg gttggcttcg ctcaacgcta aatttcctga aaaaatcaat cctgaaagac
     1741 aggtaacagc gatagccatc ggtttgctga cagggttgct tttgataggt attttcttga
     1801 ccttcaacca aaccagtagc ccaatgaaca cagcgatatc caaccaatag atgacatcat
     1861 ggggtttcat caaagaaaag gagcttcctg acaacccttg tgacactttc gaaaagccta
     1921 atacagattt gactgtaatg aaatctgtga attcccgata aaaaataata ttcaagtaca
     1981 acaaaagcgt atttaaaata tcaatgatca aaagaatggc taacgctggc tttggctttt
     2041 taatatacaa agaaaggcca aaaaacaata aggtagtcgc aatcgggttg atccacaaga
     2101 taaagtactg gatcgtacct tccacaccta atgaaaaatc tgcatagtag gcaatgatcg
     2161 tcttcagcca gaaaaagaaa acagctgtca aaacgattgc taaacgattt tggcacaatt
     2221 ttttaagttg tgtcacgtat atcacttctc tctattcaca ttttttctta ttttgctcct
     2281 gaaaaatgaa aatttcctaa aaaagttgtt atcttaagat ttttttattc tttctaaaga
     2341 ttattccatt catttttcac agcataaaat cgtttcggtg taagcggtta aaatagtaag
     2401 gagataaaaa tcttgtggtg accgatttac tctatagtat tcaaaagtag tttctcggta
     2461 ttcgtacaaa aaaaacatac taagattaga ggggaacatc attgacgaat gatgttctcc
     2521 tctattttct atttttagat tatagtgaag agtgaaagaa agttcgaaca aagccttgag
     2581 gactacgagt ccctatcaaa aaccgaactt ctttactgtc attttagaat caggtttacg
     2641 tatgttgggg ccatgagccc gtgtatagga aattggaatc gaaatgaagc agtaaagttc
     2701 tttcaacact taaaaaggag gaaaacaaat gccttacatt cttgcattag atgtttcaat
     2761 gggacacagc tacagtgtcc tatacaaaga cgacacctgc ttatttgaag acgagatcga
     2821 acacactcaa agaggatttc atgcattact cgaagagatc cagcacttgc ctgaacgtcc
     2881 attgattgtt tttgaagcaa cgggtattta ttccagacca gtcgaaaagt tctttcaaga
     2941 tcatcacttt tcttactgtt tgttaaaccc gttagaagcg aaaaaacaga tggaagaaaa
     3001 caccttacgc agttggaaaa cagataaatc ggatgcccat cggctcgcac agactcatct
     3061 taaaaatcaa cgtcaaccaa aagaagtaca aagcaatttt tacttagaga tgcgcgattt
     3121 agcccgtttt tatcaagaaa ttgagaagaa aatcactcga ctgcgcatgg atttacacaa
     3181 ctgccttcag ttgacttttc cagagcttga acagttcttt tcaaacaggc tgacccctta
     3241 cgcattgaca ttgatccgtc tttttcccca tccagatttt gtattggcat ctactcggac
     3301 aaaaataaaa aataaactga tcaacgaaac acgggaaaaa atctccgcga atcgtgccga
     3361 gcaaaaggca gatcaaatca tccactatgc acagtgtgcc tatccagctg ttgaaaaaga
     3421 cagtattcac tgtcaaaaaa cgatctatta cgccgaactt ctccaagatt tactcgaaca
     3481 aaaaaaagca ctggcgacac aaatgatcaa aaaagccgaa gcctctcctt gctttttgtt
     3541 gtaccaaacg ttccctggga ttggcgcact cacagccgct ttattgctcg gtgaattagg
     3601 agatatcaca cgatttaaaa cccataaaca attgaatgcc tttgtcggaa tcgacatccg
     3661 acggtatcat tcaggtaagt atactggcca agaccgcatc aataagcgtg gtaatcccaa
     3721 agcacggaaa attattttct tcaccattcg aaatatgatt cgccagcaac gcgcagctcc
     3781 taatcatatt gtcgattatt actacaaatt aaaaaagcaa cctatcccta agaaagataa
     3841 ggttgccaca gttgcttgta tgaacaagct tctgaaatgt atgcacgcta tggttagggc
     3901 acatacagag tacgactatg cgtacgcggt ctcagtggac cattaactaa accacttaca
     3961 gtataccact tctttgccca gatcgaaaat tttttacgaa cggggcttta tttagtgtgc
     4021 cttgattttg tcgctttcgt ttacgagcta tttttcttgc accttttact tgactaatcg
     4081 taggaaagag gctgtgacaa aattcttgtc gcagctccct atttcttgat gcaggacagc
     4141 tttttcacaa cctctccact aaacggtgtt caatcagctg accttcggta ttagctcaaa
     4201 aaacggcaaa atatgaggag ctattttccc gtttttttct ctaatactca ggtcaaaccg
     4261 ctgatttcac aacctctgat tcacggtgtt ccagaagtag cttcttcgga aataagccga
     4321 aattttacaa aaacttgaga agcacttttc gtaaatttct tcttatttct tgaagctgac
     4381 cacttctgtc acaacttctg tttttcattg tccataggtt tgatattgtc cttctgatga
     4441 ctcattcgta atgattgagg cttgattttc ttcatttccc gttatcgttt gtccactgcc
     4501 agaatctgaa gggcttagtt ctgtcgactc gtttttttga gaagaagcag ttgttttctt
     4561 gacttgttcg ttggtctttt tccattccgc tagtcgatcg gccaccactt ttttgtccac
     4621 tttttcagaa taagctgtaa ccacttttcc atttttcaaa cgtgctacct tgtcgccatc
     4681 atgagtcaaa agcaaaacga ccaaatgttt gtctttgaat tgatattgtc tgagagaaat
     4741 cgcactaagt acgtcatcga tattgataaa ttgattttct tcttttcctt ttggcaaata
     4801 gatgtccaat tcaccttttt cgtaattggc agttaattta tattcctccc accaataagt
     4861 tgagaattct tttttgaaat tatctactga ttctttgctt gctacgattt tttctgtttc
     4921 tttctgagca agatctgctg tcatttttcc tgaaaatgct gcggataaaa taagcagtaa
     4981 gaaaaaaccg aataaccaaa agagaagaaa cggtcttttt ttcctcatgc cttcctccac
     5041 tcctatttaa ttttgactat tctattatat acaaaaaatg aaatagaaag acaaaaaagt
     5101 tgaaaaagat cgtaaataat tgaacttctt tttcctgaag cggtaagaac gggaattttc
     5161 atacattctc tcttggctat tctttaagct tcaaatccat ttctgtctga tacatctttg
     5221 aaccaatgac ccgatttctt gatcgttctt tttccatctt gtgctaaatc tactgaaata
     5281 aaaccgtaac gattcttata ggcattggtc cacgaccagt tatccatgca tgtccacata
     5341 tgataaccta aacaattggc accttcttga atggcttgat ggacatattt cagatgagat
     5401 tgaacaaatt cgatccgata atcatcgtgg atcataccat cttccttgat aaatcgtgct
     5461 tcattttcta cacccatgcc gttttcagaa ataaaacagt cgatattccc ataatgatct
     5521 cgtacattga tcaaaatatc atagattcct ttttcataga tctcccatcc gcgataagga
     5581 ttcattttac gaccaggcat ttcataattg tcgaagtaat cctcaggcat aggaccgttt
     5641 ttagtatcga ttggtgtttc ttttgctttt attcggcgtg gttggtaata attaattccc
     5701 aaaagatcta ctgtgttttc tttaatgatc gtcaaatcat ctggatgaat ctccggcatt
     5761 agatcgagtt cttttattct ctcaatcaat tcactgggaa actcgccttt tacagctgga
     5821 tcaaggaatg aacgattgaa gaacgcatct gctattcgtg ctgctttaac atcttcggga
     5881 tttgattcgt ctcttggata actcggtgtc aaatttaaga tgattccgat cacaccgctt
     5941 tgatccattt catggtaagc tttgatggct aatgctgaag ccaacgcttc atgataacca
     6001 acttggattg cctttttcat atctgattct tccggataat gccatcgata caaatagcct
     6061 gcctcgactg gaacgattgg ttcattatgg gtaaaccatt tttttacttt cgagccaaac
     6121 aattgaaaac aactttttgc gtatttttca taggcttcaa ccgtttctcg gttcaaccat
     6181 ccgccttttt cttgcagata catcggcata tcgaaatgat acagattaac aaagggttcg
     6241 ataccgtttt caatcaactc atcaaaatag tgatgataga aacgaacggc ttcttcgttg
     6301 attgttccag attcatcagg tatcaaacgg ctccattgga tagaggttcg aaatgaatta
     6361 tgcccagtct gtttcatcaa ctgtacatcc tcttgatact tcgtataaac ttgcgaggtt
     6421 ttttcaggtc ctacttgatc aaagaacttt tgcggttcgt ttttgtacca ataatcaaaa
     6481 atattctctc cttttccatc gcctgaaatt cttccttctg tttgtggacc acttgctgca
     6541 gagccccacc aaaaattttt tggaaattga tatcttttca tcaaagcacc tctttttctt
     6601 ttttagttga ttccatgtat gatattttcc atcacaatca ctttttcatt ttcttcctct
     6661 tcttttttga ttcgtttcaa cagctgttct gctgctcgtt ccccgatttt atctgttggt
     6721 ggcgtgatcg tgatttttgg aaggatactc agatcgtttt tatctaaata atcatagtga
     6781 ctgaacgcaa tatccttccc gattctgaca ccttcttgat tgaaacattc gatggcacca
     6841 atcgacatgt tgtaattgct gacaaaaata gcagttgccc ctttttctaa taatgctttt
     6901 gccccgtaat agccgctttt tctagaatag tctcctgtaa agatctctaa atggatagac
     6961 ggatcctttt gtaccgcttc tttggctcca gctaaacgtt ctcttgctac gtaatctgtt
     7021 tgagcggcag caataaatcc tattgtttta tgtccttgtc cgccatgatg agcaatcact
     7081 tttgctacac tttctcgatc attcactact attgtatcaa tacctgctgg cccacccgga
     7141 caattcaatg cgacaactgg gatactcatt tttgtcagca tttcaaatcc agaccactct
     7201 tcttctgata agaagacgat caatccatcg atcgcatgct tcatcaggta ttcgattttt
     7261 tgttcgattt tttctggctg atcggcaaac ccgcttaata ataagctata cccattttct
     7321 tcagctactc tttcgatccc tgaaacaacc tccgaaccaa atcggttaga tatcccgttt
     7381 atcaacagtc caatagagaa acttcgatta ttcttcaatc ctttagcaat gatgttttct
     7441 ttatagtcca acgctgaaat cgcttctgat attttttcca tattcttttc cttcagctgc
     7501 tgaccgttta aatagcggga aactgttccc actgaaacac cagccatctt agccacgtca
     7561 cggatcgatg ccatacattt tcactcctgc cagttacatt tgatttcttt ttcttgttgt
     7621 tcgaccatga tgaccgcttt ctttgcataa tgccgcttgt catcaatatc catataacta
     7681 aagtcttcag aagtgagttc aaaaacaagc agtctttcct cttgagaccg caattcatct
     7741 ttacgaaaag ctttcaataa tttcgttctt tggatgacaa cgccgtcaat taatcggaca
     7801 tagacaagag tacttgtttt acctgaaaca cttcctgtat ttttaacttt aattgatatt
     7861 attactttgt caatatccgt tttgacttcc atctgactgt attcaaatgt tgtatagctt
     7921 agaccatgtc caaaaggata aaatggactt ccgcttaaat cataataatt ctcttgttta
     7981 gaagcagcac gctgatagta ataaacaggt agttgccgac tatttctagg ataagacaaa
     8041 ctcaatcgtc cgcttgggtt atttcttcca gaaatcatct gcgcaatggc ttttccaccc
     8101 tcctgtccag gaaaccatgc tgttgcaatg gcattcgtaa atgactcgat tttcatcaaa
     8161 tcgtagggtc ttccttgcac aagtatcgtg atgattggct tgcctagttg atacaattgt
     8221 tcaatcagct gttcttggta tccacctaaa gaaagagaag caacatctac attttctcca
     8281 gaatccatgt tgatcccttt cgatgaaact gccccgttac gaagaaattc catatcaaag
     8341 ttccgcgcac ttgatccacc tagaactgca atgatcacat ctgagttttg agcaagggtc
     8401 accgcttcta ataatttttc ttcctgatcc tcctgatttc ttacttcaca tccttgtgca
     8461 taagaaacct ttgattgggg aaactctgtt tttatctctt gtactatctt ttttttcaaa
     8521 cggtcttctg tttgtggtgc agagtagtct cccaacaagt ggtaaacatc gtgagcattt
     8581 ggacctataa cagcaatgtt ttttacttgt tgatccaacg gcaatatacc gtcatttttt
     8641 agtaaagtga tactttcttt tgctgtctct aaatttaagt gctctgattc ctccatgaga
     8701 tgatcccaat actcctttgg atcgtttgca aatggctgtt caaataaacc taataaaaat
     8761 ttgacagata aaactcggcg aactgcttga tctagtagct tttcatctac gacttgatta
     8821 tcaatggctt ctgcaatctt ggtataggtc tcatcccaca aactgagatc gattcctgcc
     8881 gctaatgcat atgctgcagc agtcttttta tctgtaaaaa catcggataa acgatccaaa
     8941 gccacaccgt ctgccataac gattccttga taaccgatct cttttcttaa tgtttgctca
     9001 aaaagcgctc gattgatatg acaaggtatc ccgtcgatgt cattataagc agccatgaca
     9061 cctactgcat ttcttgtact cttcaataat ggtccgtaaa tatcggagaa ctctctgcgt
     9121 ccaatcgtta ctgtgcctga attatgaccg cctaaagctt ctccttgtgc gatacagtgc
     9181 tttaacacaa caccgatctg atccttcttc ttattgattt cctctactgt ctgatcaaga
     9241 aaagaaactt tttctgaaat aatctctcct tggaaccctc gaacgaccgc ttcagaaaaa
     9301 cgagctgaga gaatcggatc ttccccaaag cactcttctg tccttcccca acgaggatct
     9361 tttgccagat ccaaagtcga caccagcgct aaatgaacac ctttagcagc cagttctttt
     9421 gccatcaatc gactcgattg ttctaataat gctacattga aagtatttcc acggccgata
     9481 ttcgttggat aagaaatccc atccaagcct tgatgaccat gtggacattc ttctacaagc
     9541 aaaactggaa ttttccatcg cgaatgctgt agtacatatt cttggactag gttagccacc
     9601 ttccagcttt cttttgcagg aatcccattc ttataatctg ttttagacca cggatcagag
     9661 cgaaacagac cgtataaggc acccagaccg cctccccatt taacatgttc ttttaacttt
     9721 tctgttaatt cgatcttgtc tccgtttttt tcataacatt cccaaccata caaatgctgg
     9781 tttacctgtc ctactttttc agcaatattc atcctcgaaa gtaaatcttc tactctttgg
     9841 cggatggtta actttgggtt ttgatacggg taatcgtaca ccttaatatc ctccttctgc
     9901 caattttgcc ttattagcgg ctaatacaaa tggcaggaat aaaaatacat ctagaatgat
     9961 aagtattact gttaagactg ctgctcgtat atcgcctcct gtagcaataa aagattgtat
    10021 aaccggtgga gtgatccaag gtgcagcagc aactgtttta ctgatgattc caagacttgt
    10081 ggctgcgtag gcaataacca agttaataga tggtataata gcgcatggaa tcatatatat
    10141 tggattcatg actactggta atccaaacat aattggttcc gtaatattaa ataaacaagt
    10201 agtcagacca aatttagcga ttgatcgctg ttcttgacgt ttagaagccc acaagatagc
    10261 aataactaac ggtaaaatac acccgccacc tccgatcaat ccaaatgcat tggtaaaggg
    10321 ctccgtaata atattaggaa tttcttttcc tactgaaaaa gcatccatgt tttcctgaat
    10381 agactgtaac aaaggtgctt gccgtatagg agccaaaata ctcgaaccat gcataccaaa
    10441 cacccataag aaatcagaga aaaactgtaa aaataagatg cctgggacgg ataaaacaaa
    10501 agctttcaat ggtgtctgaa tcgctaattc aattaattgc ggaacattca aaccagtcgt
    10561 atggtttaaa gtaaaaacga caactgaaaa aaataaaatg acaactattt ctggtactaa
    10621 agaattaaat gacgcagcta ctgccggtgg aactccttct ggcatcctga ttttaaaact
    10681 cttcattttc gctaatttga taaataattc tgtactgaca agggcagcaa tcatcgccgt
    10741 tgctaagcca gacgaagaag tcagactttg taagaaaacc cctgtagctt ctgctgtatt
    10801 tccgtccatc aaagtgactg tacttgataa agacatcaaa ataaacatta cggctgtact
    10861 tagaattcct gcatgaacag gactaaatga tggatcatca atcacgcctg tttggatata
    10921 gtgggttgcc aaattccagc cgatattgta acacacgata atagctaaaa tactcatcgt
    10981 accattattc acgattgaag caagttcagc caatggactt agatcaagta ttctttggac
    11041 tgttgcattt gaaaaaatca gagcattcag caaaaccatg acagaagcag ccattactaa
    11101 cggcatagta acgataaaac tgtctcgaat ggccagcaag tgtcgctggc tactcatctt
    11161 agatacgatt ggtagcattt tttcagatag tctgtctacg actttcatta tttcatctcc
    11221 tagattctaa aattgaaccg gttcattttt aatatagcac tttttttaaa gattgcaaca
    11281 cattttttta aaacgctttc taaaggtggt ggatttttta tgacttttga aaaattattc
    11341 tatttaatgg tattttgtga attttttctc ttccatccgt ttgaagtttt actttccaat
    11401 ggtataattt ctttatccta tcaaatgggg gaagtatttt gaagaaatta atcgtactat
    11461 ttattttgtc tgcgggactt ttgacattga ctggctgtga tttgtttggc ggctctgacc
    11521 aatcctcgga cgaaacaacc gattcttctg tcttaaaagc ggaaaaagca gatatcacca
    11581 acagtgtata tacgctaaaa gtacgtaaag atatggattt atccgcggga cagttgacat
    11641 ttacagataa aaaaatggaa tggacacgca cgtatcagac acaaggagct gattcttctg
    11701 aatcatctga agaaaaaaca gtgctgacag atatcaagat tactaccaaa gatgacgaat
    11761 atattatttc aggaaaagaa aataaaaaag aagcaacagt cacatttacc aaaatcggta
    11821 attaccgttt gaaggataag gatggcaaca tctacagtct atagtgccat ctgttggatc
    11881 ttcttttgtt tctccccttc ttttctgcta tagtaaagaa gcaagatgac tgtaaaggag
    11941 acgaaatgga tgatcgatag tcctgattat tgtacatgta ataacaacaa aaatatccat
    12001 acagaattta atgatcaagg aagtttgagt atatgcgaca attgccacaa actagtcaaa
    12061 gattctttta aatcgtttga atcttatcct gtcaaaaatt atgtaaaaag caaaagaaga
    12121 taagcaaaat caagcaaaac tcactatggc tgatttttat gaacttcaaa ctcagcttct
    12181 tcctttgatc aagcttcaaa aactggaatc agtaaaacaa acagtggatc atttcagttc
    12241 acaaatcaaa gagtaacgtt ttatttattt cacatctttg aatataaaat aaccaaacga
    12301 tagaaagact tattctacat cacgtagtaa tttttctttc ccgtcgtttg gttttttact
    12361 tttagcctaa ttcttgacct ttcgcatgct gattatctgt aaaatatttt ccagcaggct
    12421 ttccttccaa ttcagctaat ttttcttgga tctgacgaat cacctttttt gcatcttctg
    12481 gattttctac aaaatcgaat gtatccccat ccacttgtat tttaggacat acatcaaaat
    12541 cctcatacca ttgattatag cgggtgttca gtgtttcgta ataggtgtat aacgtttcat
    12601 cgctttctag ctgttcatag tcacgcccgc gttttttgat acgttctagc atcgtgtcca
    12661 aagaaacttt gatatggacc atcaaatcag gacgtttttt tggagcaaca tcatctaact
    12721 cgtttagcat cgtatctaat aagctatcgt actgctgtac ttcgatatcc gttacccgac
    12781 ctaaatcagc atttagatgg aacaacagac tatcttcgta gatggagcgg tctagcacat
    12841 tgtcatcgtt cactaaagca tttttcattg ctaaaaatcg cttattcaaa aagaagattt
    12901 gcaataaaaa tgcgtactgc tcaggatttg aataaaaaag cggcaatact tcgttatctt
    12961 cgacattttc ataaaatggt ttagaatcaa tctcttttgc aagcatatcc gtcaaactgc
    13021 tcttacctgc accaatcgtt cctgaaatca aaatcactga catacaaccc ttcctcttct
    13081 ataagtactc gcctacgtaa gtttcaacag gcaatttttc aaaatcatat tcacgtacag
    13141 cttggtctga ttttaaatac gtgtgaagac tttgtgtaat catcaagttc gtaggtaccg
    13201 tctcttcttg tacgatgatc atcggttttt tcaacatagt tgccacacct aattcgtaag
    13261 ccgttcctgg atcgattgtt tgatgctcaa agtcaaggat agccaaaaca aattctgcat
    13321 ttgtcaactc ttccatatct ttttcgtata cttcctgcgc ccatccagcg ctgaatagtt
    13381 cataattgct ttcttggtga tgacgtggag agtagaaact tgtgattgtt ttgttttcct
    13441 ccaacgcttt ttcgatacga cttactcgat cgatttgctc ctctgaaaaa aatggtcctg
    13501 ctaaatatat ttgactcatt attttgcctc cttcaataat aactgatttt taatagatac
    13561 tgctcgtaat tcattgacca gttccaatgg attgttggaa aagatcactt gatccaacca
    13621 ttttttatcc ataaaatcct cttttgcaaa agtttctaat aattgttgca agccgctgta
    13681 ataaccgcca atattcaata ttccgattgg cttatcaggt gtcaggccga tcgctatcca
    13741 agaaagaagt tgcgaaaatt cctccaatgt acctagacct ccagggaaaa cgaggacagc
    13801 atctcctttt tcaaataata atacctttct ttcatcaatt gtctctgtag gaataaatgt
    13861 agtaacatct ttcccaggca gctcagtttc aaaaaggcca tttggataga tacctgtgac
    13921 ttttccgtta ttttccagta ctgttttact aatcactccc ataatcccgg agtatgaacc
    13981 accgtaaacc agttcgattt cttcttgtgc catatatttt cctaacgttt gcgcaataaa
    14041 tttgtaactt tctttattgc caaaacggga accgcaaaaa acagtaagtt tcataaaaaa
    14101 acactcccta actcaaaagt ctatcttcat tctacacgtg tttttttaaa ataaaagact
    14161 agattttgta gttttttagt ggtatataga tcaaacacca ctatatatag tatttgcaaa
    14221 aaaataattt ttcagctatt ttaca
//
LOCUS       DAZUMV010000067        14056 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000067 DAZUMV010000000
VERSION     DAZUMV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.067"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..1380
                     /locus_tag="ACG8FK_001726"
     CDS             40..1380
                     /locus_tag="ACG8FK_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5097331.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1575..2018
                     /locus_tag="ACG8FK_001727"
     CDS             1575..2018
                     /locus_tag="ACG8FK_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS5097332.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2280..3698)
                     /gene="nhaC"
                     /locus_tag="ACG8FK_001728"
     CDS             complement(2280..3698)
                     /gene="nhaC"
                     /locus_tag="ACG8FK_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5097333.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4102..5325)
                     /locus_tag="ACG8FK_001729"
     CDS             complement(4102..5325)
                     /locus_tag="ACG8FK_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5097334.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5357..6196)
                     /locus_tag="ACG8FK_001730"
     CDS             complement(5357..6196)
                     /locus_tag="ACG8FK_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5097335.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6213..6893)
                     /locus_tag="ACG8FK_001731"
     CDS             complement(6213..6893)
                     /locus_tag="ACG8FK_001731"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5097336.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(6895..7968)
                     /locus_tag="ACG8FK_001732"
     CDS             complement(6895..7968)
                     /locus_tag="ACG8FK_001732"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5097337.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(8027..8265)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8308..8541)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8618..9289)
                     /locus_tag="ACG8FK_001733"
     CDS             complement(8618..9289)
                     /locus_tag="ACG8FK_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5097338.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KKWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9377..12025)
                     /gene="mgtA"
                     /locus_tag="ACG8FK_001734"
     CDS             complement(9377..12025)
                     /gene="mgtA"
                     /locus_tag="ACG8FK_001734"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS5097339.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASPNGLSEEQIERSRET
                     YGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELITGEKI
                     SEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGKFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLMPLPGNYWLWF
                     FITVVAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12368..12542)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12715..13152
                     /gene="ndk"
                     /locus_tag="ACG8FK_001735"
     CDS             12715..13152
                     /gene="ndk"
                     /locus_tag="ACG8FK_001735"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS5097340.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(13174..14040)
                     /locus_tag="ACG8FK_001736"
     CDS             complement(13174..14040)
                     /locus_tag="ACG8FK_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5097341.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
BASE COUNT         3992 a         2982 c         2494 g         4588 t
ORIGIN      
        1 atgcattttg attgtgaatt taaaagaaag aggttattta tggaagataa aaaattgaaa
       61 aaacaactta gctcccgaca tatcaccatg ctagctttag gtggagcgat tggtgcaggt
      121 ctatttaaag gaagtggcga agcgattgga atcgccggcc cttctgtatt gatcgctttt
      181 ttgattggcg gcataatttt atatatcgtg atgaaaggtc tgggaaaaat tgttttaagt
      241 ggtggtgata cgcaccacgg attatctgga ttgattcgtc catatctagg tgctcatgct
      301 gccgatttta ccgattgggt ctactggtcc atgtggatta tcaatatcat tgctgaagct
      361 gttgcagctg cttccttttt acagttgtgg ttcccccaca tcccagcttg ggtatttgtt
      421 tttatgttgg ctgttttaac tactatcatc aatctttatt cggtacgatt attcgcagag
      481 acagaatatt ggctggcctt tgctaaaatc agtgtcatta tcctattgat catttttgct
      541 gcttatcttg tcggtcagca aatgttgggt actggtgttt tccctaccct tcaacaatta
      601 actgatcatg gtggttttac tccccacggt atgaagggaa tcatcagttc tttgcttgta
      661 gttatctatt cttacggcgg ttctgagttg attgctatca cagtaagcga agcagatgat
      721 cctaaacgtg cgattcctaa agcaatcaaa ggcgtcatgg gaagaatcat ttccttttac
      781 attattcctt tatttttgct tttgattatt tttccttgga atactttagc aggcacgaac
      841 gttagtcctt ttgtgatggt atttgaaaaa atgaatattc catttgcggc agatatcgtc
      901 aatttcgtga ttgttcttgc tctgttctcg tctatcaatt caggtgtata tgcttcttcg
      961 agaattttgt tcttccgctt gaaagatcgg aaaggttcct ctagaaaact agctgtttta
     1021 aacaagcatc aagtaccgca acgtgctgtg ctgttttgta ccagtgttct ttatctaggt
     1081 gtggctcttt cttattttgt cggtgacaaa ttatttggct atctggcagg ttctctttcc
     1141 tatacagtat tattgatctg gatcattatc agtgctgcgt cctttgtact gacactaagg
     1201 aaaggaacgt tatgggacaa aggaatcagc ttttttgcat tagcagtctt atggttgatt
     1261 ttccttggta tcctattcac gaactccatt ggggtaactg tattgaccgg tttgctttac
     1321 ctgttcattt atttcagtta tcaaaaaaag aatgatgctt tcgtcttgac gaatgaatga
     1381 aaccaataga gaattttatt ctatttatat tgaatatctg gagctattga aaaaaaggag
     1441 ctgcgacaaa aattgttgca gttctttttt ccgaataaac ggtgttcaaa aaccatctgt
     1501 gcagattaat tgattgttct ccacgctcat gacatgatag aagaaaggaa tgaagaaaaa
     1561 ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt gatgattggt gtttcgacgg
     1621 tagtcatgtt tggtatgatg tatttgaatg tttatgcgct tgatcatatc ttcttcagtc
     1681 gcacacgtgt attcatggcg ttgatgatgg gtgcagtcat ggcagtcatc atgttgttgt
     1741 tcatgtggaa aatgtatgaa aacaaaaaga taaatgctat gattttagcc gctagtcttc
     1801 ttgtttttgc cggttcctta tttatggtac gaagccaaac aactgtcaac gatacggcgt
     1861 ggatgaaggc gatgatccct catcactcta ttgctatctt gacaagtaaa cgagctgatt
     1921 taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat tcaggcacaa gaagaagaaa
     1981 tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc attatccatc caaataaaaa
     2041 aagaggatgt tccagaagtg atcaacttca agaaataaga agaagctact tctggaacac
     2101 cgtgaatcag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     2161 aatagcttct catatttttc gtttggttag gcttattacc gcaaatgcaa gcttttgaac
     2221 accgtttatt cgtaaaaggg agtgtgacaa aagtcttgtc acactccctt tttacgtttt
     2281 tattgttcta attgcgctgt cttgcttact gcttcttgcg gtttcacaaa tttctttgag
     2341 atcccccaac ctgtaaaggc actgaccatc gaaaacacag gtgaaagcaa actgaaaaac
     2401 acgaatggca agtaagtgag tgtaggaatg ccaaatgtac ttgctacgaa acttccggcg
     2461 attccccaag gcactaagta gttgattact gttccaccat cttctagtgt tctccctaat
     2521 acagttggat caaatcctgc tttttcgtaa gtttctttaa atgcattacc aggcaggata
     2581 acagatagaa attgttctcc aataaagata tttactccga tgcttgtcat caatgttgta
     2641 aaaatcaatt ttggggtgtt cgtcatcttt cttgatactc gttccataac ggttgagatc
     2701 aacccaaatt cgatcaacaa accacctaat gacaatgtta gaataatcaa tgacacagtt
     2761 ggcatcatgc tttcgattcc tccacggcta agtagtgaat cgatttgttg gtttccagta
     2821 tttgaaacaa atcccgattc gatgacccct gccaacttcg ttaaggaagt ttgtgggttt
     2881 tgaataaaga tcattcctac tgctacagca atattcaaca aaatcgtcaa aattgccggt
     2941 acctttttcc atgcacacaa gagcattaaa aatagaggaa tcagtgacca gatcgaaata
     3001 gtaaagtttg cttctaaaat atcagtcact tcacgaatag atgaaagact tgcggcttta
     3061 cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg aacagttgac
     3121 cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc tgctaaattt
     3181 gttgattcgg ataatggaga catcttgtct ccaaaaacgg cacctgaaac aatggctcca
     3241 acaacaaggg ctggatttag tcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc
     3301 gttgacataa cagtaaaggc actccctaca gcgctaccta cgattgcaca aacaacaaat
     3361 acagaaggga cgaaccactg gacactaatc aatttaaatc caatgaccat gagggttgga
     3421 ataattcctg cttgtatcca tacagcaatc aatgaaccga ctaaaataaa aataaagatt
     3481 ggaatgatcc ccggtttgat gccattgatg atcccttcgt gcagccaatc tgtcgggtat
     3541 ttttttatca ctgcgtacaa catgaccatt ccgatagctg ccaaaatcgt gatattaggt
     3601 gaaaccttta atccgacaac gccaatcgca atgcctaaaa caatgaccat caatacaagt
     3661 gttgcttcta aaaaacttgg tgttcctttt ttttccatga taatctctcc ttttttagtt
     3721 gttcttatgt tctaaaaaag ttaagatatc ggattactcc gccacaattc atccgcgatt
     3781 cctcctttat aaaaataaaa agacctgctt tcaactaagg acgaatgact ccgcggtacc
     3841 accttaatta aaaacagatc tgtttttcac tttcaaacga ttattcaaat gtatggactt
     3901 tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg accaccgact ttctgaacaa
     3961 aacacctaac cactactgcg tgaaaagttg acttgtatga agtttacctt ccataagatc
     4021 atttgtcaat gaaaaagaaa atattttttc atttttctga aaattatttt tataactttc
     4081 gcgcacaact ttatcttttt actagaaagt agcgccctct tgtttttttc ttgctactat
     4141 ttcttgatgt tcttgtttga ttttttctaa tagcttgggt tctctcaata aatctagagc
     4201 tgtattggca agtaattccg ctgcaatcgc aatagaagca agtccttttt cacttttagc
     4261 agcagctttg aattcttctg tatgtccggc aatatatgtc tcagagattg ctacagtagg
     4321 ctgtatcgtt ggaataactt ggctaacatt tcctacatca gaagaaccta gacttgttct
     4381 ttgttccgtt tctatttctt catcaggaac ccctgcttct tttgcatgtt ggaagaataa
     4441 gtcatcgaaa gaaggcgtta cgatgacatc atcgactgca ttttggaaca atccaaactc
     4501 aaaagtcgta cccgtgctta atgcagcacc tcgtacgata ttttccactt tttcatatac
     4561 tttgtctaat gtttgtcgat ttcctgcacg taaatagaaa cgtccagcag cgtagtctgg
     4621 tatgacattg gccgccacgc ctccatctgt aatgattcca tggatattga catcttttgg
     4681 cagttgcaag cgtagagcgt tgattccgtt aaacacttgg atcaaggctt ccaaagcatt
     4741 tactcctttt tcaggtgctg ctgctgcatg agaagcgaca ccgtaaaatt tgatatcgac
     4801 tggatcattt gctaaagatt ctgtcgtttt cccataacga aaagctggat ggacacataa
     4861 tgctgcatcg acatcctcaa aaaaaccttc ccgaacgaag cttcccttag ctgatccatt
     4921 ttcgcctcct tcttcccccg gagtaccata cacacgaatt tctccgccaa tctcatcaat
     4981 gaattgtttc actatgctgg ctgctaatac ggagtaagta ccaaataaat tatgtccgca
     5041 tgcatgacca atacctggta aagcatcaaa ttctgccaaa aaggcaattg tcggacctgg
     5101 tttattggaa atataacgtg catcaaagcc tgttcggtgc ccagctactt ctttttctac
     5161 ccagaaccct tctttttcca attgtttgat taaagctgct gaggaataaa attcataatt
     5221 acttacttca ggatggtcgt ggatagctag tgcaagtgct tgatattctg gtaaacggta
     5281 ttgaatcaga tccttcaatt gatttgttga gagtgtggtt gtcataaaaa tttcctccta
     5341 tgctagtcgt tttttcttat tttctcccga atgtttccca cgcaggtacg cttgaacctt
     5401 ttgatgtttt ttcgattact ttggctgttt cttctgtttg ataggcatct actatttttt
     5461 gatacgtttt attgtcttca tcttcttttc ttgcaacgat gatattgaca taaggacgtg
     5521 catgctcatc gactggttct aagaagattg catcagaagc gggattaaat ccagcatcta
     5581 cagctacacc gctattgatg actgaaatat ctacatctga caaagcacgt gctgtttggg
     5641 aagcatctaa ctcagaaatg tctagatttt ttttattctc ggtaatatca gaaacggtag
     5701 gcgcttggcc ttttgccttg tccactttga tcaatccagc agtttgcaat aacagaagag
     5761 ctctgccgcc attcgtgaca tcattaggga ttgcaacttt tgctccatct ttgatatcag
     5821 ccacatcttt taacgtattg gaataaattc ccaacggtgc attaaccgta ttcccaatcg
     5881 atactaaatc agttccgtgt tcttcattgt aattgtctaa aaagaattga tgttggaagg
     5941 aattcaaatc gatcgatcca tctgctaaag ccgcatttgg ctgattgtaa tctgtaaatt
     6001 tcacatattc caaatggatg ttttcctttt tcaaacggtc tttcacatcg tcccatacat
     6061 ctgtatcttc cccaatgatc cctagtttta cggtcgtttc ttttttttca gttgtagctg
     6121 cttttccttg gctgcatcct gccaaccaaa taattgctac aaatgtaatg actgcaaaac
     6181 gaataatttt tttcattttt tctcctccat tttcaatgtg tgactttttt aatcagttga
     6241 ttactgataa attgactcaa aaaaactaag accagaatca ataatgtagc gacaatcgtt
     6301 acatctgttt ggaatcgatt atatcctctc gatatagcaa gattccctaa tcctccggca
     6361 cctactgctc cagccatggt agtcagtccg atcacattga tgatcgtcaa cgctgagacc
     6421 cgaatgatac caggtaaccc ttctaacaaa taaacacgta aaatgatgcc gattgggctc
     6481 gtgcccatcg cttctgctgc ttcgatcacc ccaggatcaa cttctagcag tgcattctcg
     6541 atttgacggg caaaaaacgg gacaacgcca aaaatcaaag ggactaaagc tgctttggca
     6601 ccaatcgtcg ttccaactaa gaaacgagta gtcaaagcta aaagagaaag caagataatg
     6661 aaagggatgg aacgaatgat attgatcaac ttatcaagta tttcgaacag cagcctgtta
     6721 gctagtaatc catttggacg tgtcactact aacgtcacac ctaagatgat tccgatgatc
     6781 ccaccaatga atgccgtcca aaaagacata tatagtgttt gctttgtcgc ttcgatgaat
     6841 tcatccggaa tcaaatagac attcggcaaa tatgttttaa aaaattcagt cattttagtt
     6901 tcctccttcg atgactttga tacggttatc taattcattt ttttgtaaaa tagttacttt
     6961 tacaccagat ttaatcaaat aatccaatgc tttttgccgc tgtttggttt ctccagctaa
     7021 agtaacgatc aaacttccta agggtgtttc ttgtaatagc tctacattac cgtataagat
     7081 attcgctgtt acttggtact tgctgtaaag atgagcgatt agaggttcgt tcgtctggtt
     7141 gccgatataa cttaattcca ccaaccattt atttgcaatc tgatcggcaa aagcagcatg
     7201 ggcactgatt gtttccaatg cttggtcaag gtgtgtcgct gtccgaataa aatctttagt
     7261 caattcttct tcaggatgac tgaaaatctg gatgctgcta cctttttcaa tgattttccc
     7321 cgcttccata accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat
     7381 caggacgatt gttagaccta gctgacgatt tagttttttt aacagtgcaa ggatctgaag
     7441 ggtcgttttc ggatcaagtg cactagttgc ttcatcgcat aacaacactt taggttcatt
     7501 tgccagagct cgtgcgattg ccactcgttg tttttgcccc cccgacagtt gtttaggata
     7561 cgcagatgct ttttcttcca gaccgacaag ttccagtagt tcattcactt tttgacgacg
     7621 ttcctgtttt gattttccag aatactttag agaaaaatct acgttgtcaa aaatcgttcg
     7681 actttccatt agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa
     7741 ttcttttgga gagagctggc ttaaattcgt ctgattgacg atgacttctc ctgctgttgg
     7801 tttctgcaac aaattcatca cgcggactaa cgtactcttt cctgcacctg agtaaccgac
     7861 gatcccataa acatcccctt tttcaatcgt taaatctaca gaatctactg cctgtatctc
     7921 tttttctttt tgtttgaaac atacagaaat atctcgcaat tcaatcatta acattcctcc
     7981 ttgtctataa aaaaagcatc ctcaagaaaa tgaaaatagt cattttcttg aggacgcctt
     8041 tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct tcaagtacca
     8101 tcatactcta gcaagatatc ggtcgcagcc gtcaccgcct tacatctatg ctcgggatga
     8161 attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta ccgaagctct
     8221 ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc aaaaaaaaaa
     8281 tccttttact aacataaact atgtcagtaa aaggacgacg tatcgtggta ccaccttttt
     8341 tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg ttcgttttaa
     8401 cggtcgaaca tttccgaatt tgcctactta tcgacaaatt attctccaag gccattttca
     8461 gcttttctta cttgactctt cacaccagcc agagcctctc tgcaaagttt ccaagcttac
     8521 tttccttctc aacgaaatca atattttgtt gttgggaata atctacagga ggaaaactga
     8581 aaagtcaagc atgaaatcca aattattttt tctatattta tttttctgtt tttttatcta
     8641 acatttcaat aacagttcga agatcttttc cttccgcaag aaggttcaat ttttccttct
     8701 cttcgacgct ataatttcga acaaaactgg aatcgggaat caaaacaacg cctagtcctg
     8761 cacgtgaagc ggctgtcgct cctactaaag aatcttccac tgcaaaagct gaaagagctt
     8821 ctaatcctgt tcgttccaaa gctgtcagat aagggactgg tgaaggcttg ttctccgata
     8881 cttcatctcc gaacgtaaac gtatcaaagt aatcggctaa ttgaaagtga tccaagatat
     8941 ctgttgctct tttcgtaaca gttgtcgtag caatccctag tttcatatga cgttctttta
     9001 agtctgtcaa tatttctttt gcatagggtt tcaattggat agctccactt gtcagctgtt
     9061 gataaaaata ttcttcacgc tttgctcgga cttcctgcac tttttcagga ctgcctgctg
     9121 cctgcactaa aatatcaaaa ctttgtttcc atttttttcc gccccaagcg tcaacgattt
     9181 ctttcgggat atgaatatca aattgctgga atccccaaag ccaaccatcc cgatacgttc
     9241 tttccgtatc cactaacaaa ccatctaaat caaatattgc acctttcatc tatacattaa
     9301 tcctttccaa aacaatacac ctagtttagc atgactgatc caaaatgaac agaagcatag
     9361 agcccagccg catcacttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
     9421 ctgtcacaag cgccagataa gcgacgactg ttataaaaaa ccataaccag taattgccag
     9481 ggagcggcat caagcctaac tcagcaccaa atcctgtgaa tggaaggact gaaccgacaa
     9541 tgattcctaa tgtagtgata accagcatgg caaatgttgc attgctttgg ataaatggta
     9601 ttttcggtgt acgcaatgca tggagtacta atgtttgtgt ccacagggat tcgacaaacc
     9661 atcctgcatg gaataatgcg ataaaaatca cttgttgtga gctatccaat gtatggaaac
     9721 ttccaccaac tacagcagga caaataacaa aatacatcaa caagtaagta gtgatatcaa
     9781 aaatcgaact tgttggacca aaataaacca tgaattttcc aatactagat gcattccatt
     9841 tctttggctc atccaaatat tctttatcca tccgatccca tggtaaggaa atgcaagaaa
     9901 cgtcatagat cagattcaaa aatagaagtt gcaaaggcaa cattggtaaa aatggtaaga
     9961 acgtactggc aatcagtacg gaaaacatat tgccaaaatt agaacttgct gttgccttga
    10021 tatatttcat gatgtttcca aaagtttctc ttccagaaag aaggcctctt tccaaaatcg
    10081 tcaaatcttt ttctaaaagg atcacgtctg ctgattcttt tgcgatatcc acagctgtat
    10141 ccacggaaat accgatatca gccactttca ttgctggagc atcattgata ccatctccta
    10201 aaaatccgac agtatgccca tttttacgta aagctgtcgt caatcgtgct ttttgctgag
    10261 gattcaattt tacgaatact tcgtgttctt cagctacttg cgctaattca tgatcattca
    10321 tttccgagat tttctcacca gtaatcaatt cattgatttc taggcctact tgacggcaca
    10381 cagaccgtgt gactaattca ttgtccccag tcagaacttt tacagctaca ccatgttctt
    10441 ttaaagcttt caacgcttgt tttgttgttt cttttggtgg atcaaggaaa gcaagataac
    10501 caatcagaac catgtctgac tcatctttga ctgaaaattc tccaacaacg cttggattgg
    10561 tcttttgtgc gacaccaatc actcgtaagc cgtcttcatt aagtgattgt acttttgcaa
    10621 gcaccgcttc ttttcgttct tttgttaatg gtgcgacagt accaccaaga tcaataaatt
    10681 tagaaatgtt caacatttct tcaatcgcgc cttttgtgat catctgtgtt ttgccagccg
    10741 gatcagcgac tacaacactc atacggcgac gttcaaaatc aaaaggaatt tcatctactt
    10801 tttgataatt gattgtatct gtatctaaag tttctgctgc ttcatcaatg atagccacat
    10861 ctaacaaatt tttcagccct gtttgatagt agctgtttag ataagcatgg cgcaaaacac
    10921 gatcatcttc atttccttca caatccatat gatattcaag aatgattttg tcttgtgtca
    10981 atgttcccgt tttatccgta cataacacat cgatcgcacc aaaattctgg atagcattta
    11041 aatttttgat gactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    11101 taacgatcat tggaagcatt tcaggcgtta aaccaactgc cacagataat ccaaaaagaa
    11161 aagcttctaa ccaatctcct tttgtaagcc cattgataac gataactgct ggagccatca
    11221 atgacataaa tttgatcagc agcatggatg ttttgtggat gcctaattca aaacttgatt
    11281 ggatgggttt ttcagatagc gtttctgcta catgcccaaa tagtgtgtca tttccgacag
    11341 aaacaatgat tccttccgcg cttccactga tgacattgct tcccataaag acaagatttt
    11401 cataacttgt ttcactttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    11461 agctttcccc tgtcaatgct gcttgagaaa tgaatagatc ctttgcggac aataaacgca
    11521 tatccgctgg tatcatatct cctgctgaaa gccgaacaag atctccacag acgatttctt
    11581 ccataggaat ctctgtatac tcgccatttc gtttgacagc tgccgttact ttcacaagtg
    11641 atttcagttt ttcagctgct tgattagaac gcacagactg gatcaacgtc atcgtcccac
    11701 tgacgataac catagtgaag ataatggcta caccaaagag atctttatct cctggagcgg
    11761 ctaatacgta ttccgtaatg aatgaaataa ttcctaatgc aatcaacact aatgtaaagg
    11821 gtgtgatgta tgctttcaat atttctataa tgaatggtgt ttttttgcca tatgaaatcg
    11881 cattttcacc ataagtttct cttgaacgct cgatttgttc ttctgacaat ccatttggtg
    11941 atgcatggaa caattcaaat aattcttgct tcgtttttcc agcaaaagct gcatagattt
    12001 tttgtgtatc tttctttttt tccatctcga tctctccttt gtgtccaaaa gacagctgtt
    12061 ctcgctgttt ttggtctttt gctattcatc ggatcgatag agaaaatgct gaaaggggct
    12121 gagaacagat agataatagc cctggccttt cctctaaatc cttgtttttc gaatgactgt
    12181 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    12241 tcgaatagtt attttcccaa ctcgctcacc tccctttttg ttttttcacc tatgggattt
    12301 gcggcaacaa aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcaaatcaaa
    12361 aaaagcttct cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatatc
    12421 tgcttttagc taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt
    12481 ctctcgacgt ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatccta
    12541 ttcggttttc cctcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg
    12601 tttttcatac ttttttaaaa tacacgcgtt atatttcctg tctcacgcag ttttatttgt
    12661 ctcattttcc acctcccgct ataatagaat tatagtagga aacgaggtgc tgttatggaa
    12721 cgaacacttg ttattatcaa gccagacggc gtaagacgcc atttggtcgg aagcatcatc
    12781 caacgttttg aagcaaaggg actggccatt gctgaaatga aatttgcaac gatgacacct
    12841 gaactagcaa aagaacatta tcagcacttg actgaacgta gtttttttga tgagcttatc
    12901 gattacatga cttctggtcc ggtcgtttat cttgtgctgg tcggcgagga agtgatcgat
    12961 atcgtccgta agatggtcgg agcaacaaaa ggggcggatg ccgttccagg tacgatccga
    13021 ggagattatg cactgccggg gactgaaaac atcatccatg cttctgattc ccgtgatgct
    13081 gcaacaaaag agatcgcccg ctttttcccg ccattcgata caaacaaacc gtctgaaaaa
    13141 gcggcgaagt gatacaaatc ctaaactgtt ggtctaactg ctgggtttag caatggccag
    13201 cagttttttc tctaaagaat tcctttgatt cttccctaat gtatattcga tcactgcgaa
    13261 aaactcttgg tgattttccg atttttcttc ccctaaagca gcagaaatca gtcgttggta
    13321 ataatccgcc atcaatgttt cataaatcac tgtacttctt tctttatgcg ctaaactccg
    13381 tttcatatct tcattcacgc tacgtgcctt ttttaagcga ccattttcta tataaaacaa
    13441 agtcaagttc gtcaaaaaag taaagagata ctgctcgtga ttattcagtt gcgcgatccg
    13501 tcgcattctt tttttgattt tcaaataatt attctcaata aaacgatcac tgaatatcca
    13561 aggaaaatct gctagaatag atacttcaaa ttgcccatac tctggtactt gcatcaaata
    13621 ttcttcaatg atacgacatt cataacgcaa aaattcaaat tccgtctcag atacttctgt
    13681 ttttttggac atgtgcagat tcaaaaccaa atagtacaga tcttctgttt tcttatagtg
    13741 cctgcggtta ttagcaattt ctttgtaagg atcaaagctg tcttcttgaa tccgatctag
    13801 cattcgtctt tttttcttta atttttttgg ctcatcaagc tgatttcgta tccattcata
    13861 ttcggcaagt gtcatgttca ttcgctctaa aaatgatttc aatgttccaa acgatacttt
    13921 tttcactgaa tttgtctcga tcccgactag cgaagtttta gaacagattc cttccgctaa
    13981 ttgggtctgt gtcagctttt tgttgactcg aagcttctta atcaattctc catcatacat
    14041 aatagttctc cttttt
//
LOCUS       DAZUMV010000068        13934 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000068 DAZUMV010000000
VERSION     DAZUMV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.068"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..209)
                     /locus_tag="ACG8FK_001737"
     CDS             complement(<1..209)
                     /locus_tag="ACG8FK_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5097342.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWE"
     gene            complement(688..1407)
                     /locus_tag="ACG8FK_001738"
     CDS             complement(688..1407)
                     /locus_tag="ACG8FK_001738"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5097343.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1456..3102)
                     /locus_tag="ACG8FK_001739"
     CDS             complement(1456..3102)
                     /locus_tag="ACG8FK_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS5097344.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3281..3871
                     /locus_tag="ACG8FK_001740"
     CDS             3281..3871
                     /locus_tag="ACG8FK_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5097345.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3963..5468
                     /locus_tag="ACG8FK_001741"
     CDS             3963..5468
                     /locus_tag="ACG8FK_001741"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS5097346.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5552..6904)
                     /gene="celB"
                     /locus_tag="ACG8FK_001742"
     CDS             complement(5552..6904)
                     /gene="celB"
                     /locus_tag="ACG8FK_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5097347.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6904..7422)
                     /locus_tag="ACG8FK_001743"
     CDS             complement(6904..7422)
                     /locus_tag="ACG8FK_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097348.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7438..7764)
                     /locus_tag="ACG8FK_001744"
     CDS             complement(7438..7764)
                     /locus_tag="ACG8FK_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS5097349.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7777..9756)
                     /locus_tag="ACG8FK_001745"
     CDS             complement(7777..9756)
                     /locus_tag="ACG8FK_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5097350.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9777..10091)
                     /locus_tag="ACG8FK_001746"
     CDS             complement(9777..10091)
                     /locus_tag="ACG8FK_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS5097351.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10259..10552
                     /locus_tag="ACG8FK_001747"
     CDS             10259..10552
                     /locus_tag="ACG8FK_001747"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS5097352.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10630..11742)
                     /locus_tag="ACG8FK_001748"
     CDS             complement(10630..11742)
                     /locus_tag="ACG8FK_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5097353.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11895..12743
                     /locus_tag="ACG8FK_001749"
                     /pseudo
     CDS             11895..12743
                     /locus_tag="ACG8FK_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13096..>13934)
                     /locus_tag="ACG8FK_001750"
     CDS             complement(13096..>13934)
                     /locus_tag="ACG8FK_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5097354.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT         4344 a         2852 c         2385 g         4353 t
ORIGIN      
        1 ttttcccaac ccacccaagg actttccctc aagctagcca agaacccatc tgctgaaata
       61 tgaaaatttt tgaatgctac gacattcgcc attacaggta tgtaaaagaa aaaaatcatc
      121 caaagcatac ccggtacagc catcaggaca agtgctttat aacgccatag attcataaaa
      181 aaatgattga acccctttcg ttttttcatc taaaaacccc tttcgattca ttcgcaacgt
      241 aattatagcg ctttcaataa tgagaataaa taaacaaagt atagggaaaa tgaaacaaag
      301 tataggagag gttgtgaaat cagcgttttg gcctgagtat tagagaaaaa aacgggaaaa
      361 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
      421 tttatcagag gttgtgaatt cagcgttttg gcctgagtat tagggagatc gtgtccttac
      481 tatctaactc tgagtcacaa ggaacaatgg ggactgtaac aaaagtcttg tcacagtccc
      541 ttattccgaa caaacggtgt tccagaagta gcttctacgg aaataagccg aaatttcaca
      601 aaaaagccat ccgaaagtca gacggacaag tctgcttttc agatggcgct acaactactc
      661 tgcttattct tttagctatc cattcaatta tttttctctc acttgttcta gctcttcttt
      721 ggtcatgggt cgatattgcc ctttattcaa atctttaggc agccaaaatt ttcccatacg
      781 gattcttttt aaatataaga cttcttttcc tactgctttg accattcgct taacttggtg
      841 aaactttcct tcatgcaaaa tcagacggat aattgaagtt tctgtctctt gatctacttg
      901 atcaatttgg agttttgccg gcaatgtctg ctcatcgcca tcaataatca gtccttctgc
      961 aaataaacgg atatcttctt ctgtcattat accgctaacc tcagctagat attctttctc
     1021 cacatgtttt ttaggtgata gtaactgatg ggcaaacacc ccgtcattcg ttaaaatcaa
     1081 gaggccctct gtgtctttat ctaatcggcc gactggaaaa agatcttccc gataatcctg
     1141 gtcttccaat aaatcgatta cggtttgatc atgattatcc tgagtagcag aaatgactcc
     1201 tgcaggttta tgcaaaatgt agtaaaaatc tttttgatac gtaatgattt ctcctaaata
     1261 agtaaccgca tcttcgaatt catttacttg cgctttatcg cttttcacca cttgtccatt
     1321 gacttcgatc gttccttttt tgattaatcc tttgacttct ttgcggctgc caagtcccat
     1381 atctgctaaa aacttatcta aacgcatgcg tttcccccat ttcaaaagag catcaatcta
     1441 gctcttctga ttcacttatt ttatttttaa tttatgtcgt aaacgcgcta ttcctggtcc
     1501 tagtagtctt tctgccaagc ggattttcaa agctaaataa ccatacacta aagcccctat
     1561 acctgctact acaagaatca acaatagaga ctggaatttg ctatcttcgt ttaaaaaagc
     1621 accaaaaatc atctttgtga tccacgaagc caaaagcata atcaaggtaa gaataaagat
     1681 cagcaatgtc ctgcgccaga caaaacttgg tttaaagcgg gctacttcat ggattctctt
     1741 taagatcaag gtacaagaca cagcaaatcc gatcattgtc gccaataaag ggccgtatac
     1801 ttcaaataaa cgaatggctg ggtattgaag aaggagtttc actagaaacc caataaccaa
     1861 ataattaatt gccgccttgt tttcaaacat tccttgcagc atgttggaaa ctagcatata
     1921 tagtcctagg aagagtccta caaaacttgc ttggatcagt acattacttc ctagagaatc
     1981 cggtgtataa aataatgtat ttagcggata ggcaagtaac ataacaccaa atgtcgccgg
     2041 aaacataaaa aatgaaaaca actgtaaatt tccgcttgtc aattttgcca gacctactcg
     2101 atctttcaac gtaacagctt ctgtaatcaa cggtaaccca gtagacgcga tcgatgttgc
     2161 caatgcaata actaccatcg tcaacttatc tggattggca ctaaaaatcg aaaaaagatc
     2221 cagtaactgt gcattggaat actcggtgga tgcagacatg atcttcataa aagtgaactg
     2281 atcgactaat ttgaagatcg ttacccctga acccacaata ataaacggaa tcgcttcttt
     2341 aacggtatct aacaacaatt ccttcgttgc aatatttatt ttgttttcgc tgtagtgaat
     2401 aaacgccgat gtatacgctt gatgtttctt caaaaaatac aaaaggacaa gaatacttgc
     2461 tagcataccg ataaatgccg cgaaagttga ttgcgtgacc gccgtcacat agtcaccatc
     2521 catgactttc atgatgatga atgtagccaa tagcatataa aaaatacgtg ccacctgttc
     2581 tacaatctgc gacagcgcat aaggcatcat ttcttgattg ccttgcaaat accctcgtat
     2641 cacactcata caaggaaaaa ccaatacagc gatactcaaa gaacgcatcg ttggaatcag
     2701 ttcttctccg ccaccagaag catgagctag ccaaggagag gcgatgtaca tgaacaaagc
     2761 aaacaacagg cctaacccgc ccatcatctg taatgcacgg ataaataatc gtcgtgaagt
     2821 gccatattca ttcaatgaat tatagcgtgc agtttgtttt gcaatcgcag caggtattcc
     2881 tgctgtagag atcagtagaa acaatgcata tatattgtat cccatattga ataatccatt
     2941 ggcagcctta gcattttcgc ccatccaagc ataccaggga atgatataga cagcgcctag
     3001 caatcttgaa atgatattac tggcggtcat ccaagcagag cctcgtgcca ttttttcttg
     3061 cggtgttaat tgatctaatg ctggttttct ttgattatcc atcggtcaga ttccaacttt
     3121 ctacaaaaca ttactatcat tttaattggt aaaacagaaa ctggcaatcg attttatata
     3181 acttttataa gaacaattgt ttttttgaat tcggtttcgt ttttttggta aagtaaagaa
     3241 atatacagcc ttttatggct aacatttata ggagatatca atgacacaag aaataaaaaa
     3301 actacccgtt atatttttag gtcttttctt tatcacagtt ggattgaatt ggttcttgct
     3361 ccctcacgat atagcttccg ccggagtggg cgccatcgga catttagttg aaacggcctt
     3421 ttctatccaa cgctcagtta ctgtttggag catcaatctt atgatgctgc ttctagccgc
     3481 tgtcctatta ggaaaatcag tattttttaa gactgtagcg ggaagtctgc tttttcctgt
     3541 tgttttagag attgttccaa aagtggaaat gatctcctca catttctttt cactgatttc
     3601 aggaagtctt ttgttcagtc ttggggttta cacattgtac acagtcgggg cctcaaatgg
     3661 cggtataacg ataccgccga ttattttcca caaatttttt cggcttccaa tgccgcaagg
     3721 acttttactt accaatagtc ttatcgtctt tttgaattat tatgtttttg gattgttgga
     3781 gacactattc gttctcctat ctattacagt cagttcattg tttatgaaaa tactcatccg
     3841 cttgtcccct gtgtcaagaa aatttgaata aagctggatt ttgtcatagc ggaaacaaac
     3901 gatatattaa aaagagtgtg ctataataaa ccaagaaatt gaataaagcg agggatcaat
     3961 caatgagctt ctctttagaa gacatccgtc atttactatt aaaagaaaat ctattaaaag
     4021 aatttgtttc aacaagaggc tggcatcttg atgtaccaga aaaagctgaa tttcttcagt
     4081 taagttatga ttcaagaaaa gctgatgctt ctactttgtt tttctgtaaa ggaatgaatt
     4141 tcaaagaaga atatttggat tcagcaatcg aacagggtat tcaatattat gtatcagaac
     4201 aaccatacga aaattcagca gtaggaatca tcgtaacgga tatccgtaaa gcaatggcgc
     4261 ttcttgctat ggctttttac gactatccgc aaaataaatt gaaagtgatc ggattcactg
     4321 gaacaaaagg taaaacaact gctgcgtact ttacaaaagc aatcttggat cacacaacaa
     4381 ataaaaaaac agcattgctt tctactatga atacaacgtt agatggcaaa acatatttca
     4441 aatcccattt gacaacgcct gagtcattag atttatatcg tatgatgtca gaagcagttg
     4501 aaaatggtat gactcatcta gtgatggaag tttcttcaca agcttacaaa acacaacgcg
     4561 tatacggctt gactttagat gtcggaatct tcttgaatat ctcgcctgac catatcagtc
     4621 cgatcgaaca tccgacattt gatgactatt tctattgcaa gcgacaattg atcttgaatt
     4681 caaaaacagt tgttttaaat catgaaagtg actattttga tttacttagg gaaacagccg
     4741 atctattcca gattccaacg atcacttatg gtcgttcgga agaaagcaat tatcaagtga
     4801 tccgttcaga taaaggtact cacggtttta ctttatcttc tcatgaagat cgcttagcta
     4861 tctgcgatac tgcttatgat attttgctgg ctggaggatt caaccaagaa aatgcagcca
     4921 gtgcgatcat tgccgcagca ctaacaggtg cctccagtca agatgcacaa gacggattaa
     4981 aagaagctcg tgtaccaggc cgaatggatc aattgatcca atccaatgga gcgcatgtct
     5041 atgtggacta tgcacataat tatctaagct tgaaaacatt gctgaaattt gccaaaaatg
     5101 aacatccaga tggccgtgta attgttgtat taggcagccc aggaaacaaa gcaatctccc
     5161 gtcgacatga ttttggaaaa gtgctatcag aaacagctga catcgccttt ttgacggcag
     5221 atgacccagc atttgaagat ccgaaaaaaa tcgcggaaga aatcaatgaa gcaattacta
     5281 atccagattt gatcgttcat tatgaaatgg atcgtcctga agcgattcga cgcgctttag
     5341 cagaaagcgc tccgcaagat tcagtcgtta ttgcgggaaa aggcgtggat ccctatcaaa
     5401 aaatcaatgg tgtagatgaa ccttatgaag gggattatgc aatcgtgaag cggttgattg
     5461 aagaataatg gtatttatat actagtggtt tcccatttct aagaaaaaaa gggactgtga
     5521 ttttttgtca cagttccttt tttcttagaa atcattttaa cttttcacta gctaattctg
     5581 ctgccttttc tctttcttca tttaataatt tattatcata gaatttgata aatgggaacc
     5641 agataatgaa tgcgatgatt gcacaaatga ctgctaacac agcagctttc caatcaccac
     5701 cactaccaac aaatgctccg atcccgactg gtgaaggcca cggcatctgt gcaagcatcg
     5761 gtttgacgat ttccagccga atggcaaaat aagcgagtgt agcagaagcc atcggagcta
     5821 agaaaaacgg aaccgctaaa tagggattgt agacgattgg catcccgaaa atgattggtt
     5881 cattgatatt gaataatcct ggtacaagtg aggcttttcc taacactttc agctgatcag
     5941 atttcgcgag gaatgcaata aagatgatca atcctaaagt cgcacctgat ccaccaacgg
     6001 ttacataaga attgttgaat tctcctgcca acggataatt tgcgccttgt gcgttagctg
     6061 ccatattagc taatacgatc ggggtcaaga aagaagtgat gatattcgct ccatggatcc
     6121 ctacgatcca caatgcatgg atcaagaaat aaatcaccat gacaccaagc catgtattcg
     6181 tcaactgcgt aacaaaccca aatgggatag caatcatttt gaagatatcc gttcctagtg
     6241 caactaataa tccattgatg atcatcacca caaacgcaat gacaaatgtt gggatcagag
     6301 cagtgaaaga acgagaaact cctggcggaa caacatctgg catttttatg acccaatttc
     6361 tcttgacaca catacaatac aattcgaccg caataatcga cataataata gctgtaaaga
     6421 ttcctgaagt tcctaaacgt tcgacaaaag agcccatcct aataccattt actacagttt
     6481 ctgtctcatt gatagaagca atcaagctga ttttaccatc agtcatgatc aattcaggga
     6541 tacacataaa gaatgcaaaa acagatagta atgcccccgt gagcggatta acatttaatt
     6601 tttgttcatc tcgttcaatt gacgttaatt cataagcaaa cacaatggca aagtaaatag
     6661 ataaaatccc catcgtcatt gtattgacga tcatgtacaa gtcactaact ttgaaaaaag
     6721 ttgcattaaa aaatccttct aaaaatggga acggtaatgg caaaatattt aaaaccaaaa
     6781 acatcgagcc tacaatcgtg aaaggaatag aagccatccc cgctgccatg acagcccgaa
     6841 cgattttaag ttgcgcaatt ttccccatag gtcccatcag gtatttctgc aaaaaatcaa
     6901 acattagtta ttctcctttc ctaaatataa cgctttttca tattgtttaa tacggatata
     6961 ctgttggtct tggttgagtg aaattttctt taaacgatac tgtataaatg cactggcaaa
     7021 aataccgctt acaacaagaa tgaaaacaaa taactgtcct ccattgttat ttgccataaa
     7081 aggatacaag aatgtaaatc ctgaggtaaa caacactgga atcactaata aattaaagac
     7141 actctggaac aaaaaatacc actttgtcca tggaacgttc gtttggtgtt ttcgataaag
     7201 ctttacttgt tctcctactg caggcaaaat agctaaaatc agaatcatcg gtaaactact
     7261 tcctaaactt ttagctgata gaagcagaat catccattgc atgttcatga aaaagaaaaa
     7321 ggcagtcagg taacgtacga ttagataacg attgaaatac atgtttttca aactaagttg
     7381 ctttttttct aattcgattt tcctttttac tgtatcctgc acaatgatcc ttcctttcta
     7441 tttatttttt tgatacaaat aattcatttc ctgtgcaact tccaataatg tcatcgtcgt
     7501 catcagatga tcttgtgcat ggaccatgat aatttccatt tctatttttg tcccgctggc
     7561 atattctttc agaagttgag tttgtgactg atgcgcatga agaatctctt catttgcttg
     7621 tcgcaacttc tcttctactt cttcaaacat ctctttacgc atcgagcgaa atgcttcatg
     7681 tatcagtgtc cgtgcatttc cgctatggaa aatgatatca aaagaagtca cttgtagtgt
     7741 ttcactgttt attttttcag ccatctgttt tcctccttat tcgatcagtt tgataaattt
     7801 actgctgaaa ttctcaaaaa taggttcagc taagatttcc tgctgtactt ttagctggtc
     7861 aactaaccta acgattgctt tagtcacgac cgtaattcct tcattttcga cataagaagg
     7921 cgaaactaaa aaaacaaaat gaatttcttg atattgatcc cactgcatcc cagaaggaat
     7981 caatgcaacg gctacctttg tttcgattcc aactggaaca gcgggatgtg ggactgccac
     8041 tgtatcactg aatatgattt gtcccatatt ttcacgttgc tggatctgct ttcgcatttc
     8101 tcttatatat ccttcttctt catttatgct cagcttttgc agcaaatcat gaagaatttc
     8161 ttcctttttc ggttcgtacg tataagtctt gaaaaatttt tcagacaact gttcggtgta
     8221 aatctgcttt ttttgctgta gagaaaatgc tggtatcggt ctatctgagg aatgataggg
     8281 tatagattct ccaactacct ttcgtatttt acgaacatca tcttcattca aaaaaatact
     8341 gacatgtaaa actggaattt tgaaaaacat tgtcgaaaga tcaatagaag aaataatcag
     8401 gtcgattcct tttaaagttg tttcattgat ctcataatag ccttttacac ttaccactgt
     8461 aatattttta ccgaattcac tgactacgcg attctttaat agttgggcac tgccgtaacc
     8521 cgttgcacag ataatcaatg catgtagttt atgcgcagct ctttcttttt ccaatgccgc
     8581 catgagatgc aaagccaaat aggcccattc atcttcattg atggtatagt ttttcaaagc
     8641 cggcatgttg ctgaaatgcc tttttactgc gtcaaagact ttgggatttt ctttctttat
     8701 ctcctcagtc aatgggttct caatgcgaat acctcggctc aatcgaatca tcatcggtga
     8761 caaatgatcg agtagtccat tgaaaagctg gagatcctct actaatgaaa aatgaaattc
     8821 ctgagacaat cttttcagaa ctttcgctaa ttcctttgat agttcctgat cattttgatt
     8881 ttctttgtga ttcgacttag ccatgagatg taatgtcaaa taagcaattt cttcttcggg
     8941 aaacgataca cctgcgatta cctcaatacg ttgaacgatc cgctgcgcca cttgatactc
     9001 tactcgctta gaaacttctt gttcgatgcc taattccttg atttcaaacc cttctcggag
     9061 ccgtttgatc cccaaagcca agtgtaaagt cagattctgt atgataaaat ccgaaacttt
     9121 taacttagct tctcttgttt catcgagaat gataatgact agctcttcaa aactgatgtc
     9181 tgggaaaaaa cggctttgcc ctaaatattc tttcaatgaa tttccataat ggtttccaaa
     9241 aaaagtatcc atgataaaat gccgtcgatt tctttcttcc ccttcgatcc atgtcccttg
     9301 accatgctta gaaacgattt ttagtgaata tggaatcaat cgctccttga tttcttgcat
     9361 gtcctttgtc aaactggaac gactgatata aagctcttct gccaaatcat ccataaaaag
     9421 aatggcatct tccaaaagca attggttcaa tatatacttt tgtcgatctt cggactcata
     9481 aaatccttgc ggttctctag catatccagg tactatttct tgccgactca aaaacaagtc
     9541 aaatgtcagt ctttgtaaaa tatgcagttg gtagccataa cctggtttag caatgatctt
     9601 acctccgttt ttttctacta actctttgat ccgctgcaag taattacgga ttgtccgatc
     9661 agataataac aattcagaag ctaaacgttg actggtgaca tactccttct tgtgatcaat
     9721 caaatatctc aacagcaatt tttccttctg tttcattatc atccactcct attccatcat
     9781 atgttctctt cgatcacctc tgctaacttc tggatgcctg tagggattgg gacatacgct
     9841 tggaaaggga tactaacaac aggctttcca acagattctc ctaatttttt gaatttatcc
     9901 aaaaacatcg tcgtttgcgg gctgatcaga aataaatcaa aatcactatt cttaatcata
     9961 ttatcgcctt ctgtagctga aactgcatct acgagtacct cttctctttt ttgcttaaag
    10021 aaatccgttg tctttgaggc catcatcgag gatgacattc ctgccgcaca aataatcaat
    10081 gcttttttca taaatcaatc actcctttta tttttataaa ccaagcatat cttgtaagcg
    10141 ttttctattc aaaatatata tttccgtagc attgcggaaa aaatgtgtat catatcttga
    10201 ataataattt ctggcaccgt gatatactag tgatagaatt ttttcaggag gggatttagt
    10261 gagtatcaca gtgaatattt tgtatacaag tacaaatgga caagccagaa aatttgcaga
    10321 agaaatggtc gaaagaaaca ttgtcaaagc gattcgtgca gaagaaggaa acgagaaata
    10381 tgactattac tttccactag aagatccaga atctctttta ctgatcgacc gttggaaaga
    10441 tgaagaagca atcgaaaagc atcacaaatc cgaaatgatg gctcaaataa acgaactccg
    10501 taaaaagtat aaattaaaaa tgaaagtcga acgtttcact gatttaccgt aaaaagatac
    10561 acatggaatg atagcaattt atcatccatg tgtatcttca ctttttcctt attttttatt
    10621 cgatctcatc taatgattca aaccgtgttt ttctcttatt tttcggataa gtatttcagt
    10681 caccccaata tacatgattc ctaaacatac cccaaggata ttctcaaaaa tgcggatcgt
    10741 tactgctcct ggaaccccga ataaactggc agtgattgcc aaagcaccaa aagaattaaa
    10801 aactgtttta tatctatagg tagaacaaac acctaaagct agaccaccta atataccgac
    10861 ccagttaaaa ggaataaatt ggctgatacc aataaataaa acacatccaa tcaatgagcc
    10921 aatgattcgg tctactgctc gttctttgaa accaatcgac atcaatccat agctagatac
    10981 aatagaagaa aatgcaaacc ctgcccacat caatcgctga aaagggacat actctcctgc
    11041 aacgatcaaa agactaatgc caaatgcata gatgagcatc caaatttttt cctgcgaata
    11101 catgcctttc aaaaaaatct ctttaaacaa gcttttgtct ttgttctttt ctcgatgttt
    11161 tctgtaaagg agaactgaaa accaacaaaa aaacaaaaat aataagctac ttgtttgtgt
    11221 aaagaaattc ttgttcaacg aatctttagg taacgaataa acaataaaga gatatgaaaa
    11281 gccgtacaaa gaagcattcc ccattttggg atcactagcg gttgccatta atatgctgct
    11341 tagtaaaata aaatgaatca acatgcccat aactgaccat ttgatcagct ggacatacgg
    11401 tgctactaat agtgagaaca tgacgattgc taaacctatg atcgaatggg aaatcttata
    11461 accaaagtca acaaaacgaa tactcatcaa catacagaat aaaaccacag cgtatggttt
    11521 atgcttcgat tcaaataatg ttgtaattat aatgataaaa caaatggcaa acaatacgat
    11581 gaataatgat cgtaaaaaca atgaacaaat atacatgcac ttttcttttt tagaagctgc
    11641 ttctcgtatt ttttgtttta aaataaacgg atctagttgt agagactgat aaaaatccac
    11701 ctgttgtcgt cctttctttt tacttttatt ttttacagtc atctatgaca ggttgtaaat
    11761 actatggaac atcatatggc gttgtcattt tcttgtcaac tatttttcct attcatgttt
    11821 ttataaaaaa acatatcgtt ttcttgtatg attaaatcac cacaaaataa acaactgaga
    11881 gaacgatatg ctttatgaat ggttctatca aaaaaattct gagatcaata gacaaaaatt
    11941 tgatgattac agaggtctct tacgaaacct tacagaagaa aaaaacattg atcgttgctg
    12001 cagttctctt ccctgctcca tgtgtctgta gaaattgtgg ttctgctgtg gtagatggaa
    12061 ataggaaatt aatggtggtg aaaaacaaga agaaggaaac cattgtccac tttgaataat
    12121 ataatcatat gccaatggtt atacgcctaa aaaagcagcg ctatacctgt aaaaactgtc
    12181 gaagccattg gaacgctcaa agttatttta tccgtccaag gcactcgatt tcaaatcatg
    12241 tcagacataa aatcacttct ttactgactg aaaaagtgtc gttatttttt atttcgaaaa
    12301 gttgttaggt atctttgaca accgttattc gcacattgaa agagttgaga agctatttac
    12361 caaagtcttc caaaagaatt ctgtcttgag tgttgagggt tgtgaatttc gttctcacgt
    12421 ttccgttgag gataaactga gttttatttc cgcggatggc gagacagaaa aattaatcga
    12481 gaaaatttac ctacacaaaa aattcctcga ttaacaaact atttccttag ctgcactaat
    12541 cctaaagaaa tagaattctt agttacagac atgaacgcca cctactttca gctcaccaaa
    12601 catgttctac caaatgcgaa attggtgatt gatcgattac ttggtttttc cacgcctttc
    12661 aaggaggcgt aacctttatt cacgagttgg ttgaagcttt ccgagacaaa agacccgact
    12721 tgtttttctc cttattgaca aagagccatt aaaactagag aaaaaattac aactgatcgc
    12781 tgattatttg tagaaagaaa atcaaagtat tacccaataa acttgagtca ttaggcaaaa
    12841 aacactgcga aactcaagaa tgattttcgc agtgtttttg tatatttttt attaactagt
    12901 attactacaa aggtttattt ttcacttctc aacgcagcac aaaattgtct ttacttattt
    12961 actggttggg ttcaaccttc tctactccga tactaatatt tgtttgtata ccccttctaa
    13021 aatcggtagt ttccattcaa ttgatgggta ctagttgatt ttaaaaacta tgcttattca
    13081 attttttatt ttagctcata tatttcgttt ctttctaaat tttatactga tgaaaacaag
    13141 tagcacaccc aatattgaca atataaactg gctaatgatg gtttctccag tttttggaag
    13201 atgcttattt ccactgcttg ttggattgtt tcctcttata tctgagctat cagcaacttt
    13261 tctattctct tttgttaaaa agatcgccac ttcttgttct ttttcgatct tgaaagttac
    13321 tggtgtcgca tccaattcat accccgttgg cgcttgagtt tctaccagct gatatgctcc
    13381 tggtgctaag ccatctatcg ccagttttcc atctgctcct gtggttagac ctgactgcag
    13441 aacggttcca ttggcatctt gtaactcaaa aacagctcct tgcaggactt ctccgctttg
    13501 gtcatcgatc tttgtcaaca cgacaccgcc tggtgtaagt cgattttctt tcgtcaattc
    13561 taccaccgct gtctgacttc tttcgatctc aaactcgatt ggtgtcgcat ccaattcata
    13621 ccccgttggc gcttgggttt ctaccagctg atacactcct ggtgctaagc cgtcaatcgc
    13681 cagttttcca tctgctcctg tggttagacc tgactgcaga acggttccat tggcatcttg
    13741 taactcaaaa acagctcctt gcaggacttc cccgctttgg tcatcgatct ttgtcaacac
    13801 gacaccgcct ggtgtaagtc ggtttgcttt cgtcaattct accaccgctg tctgacttct
    13861 ttcgatctca aactcgatcg gtgtcgcatc taattcatac cccgttggcg cttgagtttc
    13921 taccagctga tatg
//
LOCUS       DAZUMV010000069        13829 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000069 DAZUMV010000000
VERSION     DAZUMV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.069"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            363..731
                     /locus_tag="ACG8FK_001751"
     CDS             363..731
                     /locus_tag="ACG8FK_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS5097355.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="ACG8FK_001752"
     CDS             911..1852
                     /locus_tag="ACG8FK_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS5097356.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="ACG8FK_001753"
     CDS             1921..2652
                     /locus_tag="ACG8FK_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5097357.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="ACG8FK_001754"
     CDS             2796..4976
                     /locus_tag="ACG8FK_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS5097358.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="ACG8FK_001755"
     CDS             4996..5478
                     /locus_tag="ACG8FK_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS5097359.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="ACG8FK_001756"
     CDS             5494..6255
                     /locus_tag="ACG8FK_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5097360.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /gene="murC"
                     /locus_tag="ACG8FK_001757"
     CDS             6386..7720
                     /gene="murC"
                     /locus_tag="ACG8FK_001757"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS5097361.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="ACG8FK_001758"
     CDS             8027..8512
                     /locus_tag="ACG8FK_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS5097362.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="ACG8FK_001759"
     CDS             8505..9188
                     /locus_tag="ACG8FK_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS5097363.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="ACG8FK_001760"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="ACG8FK_001760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS5097364.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="ACG8FK_001761"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="ACG8FK_001761"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS5097365.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /gene="coaE"
                     /locus_tag="ACG8FK_001762"
     CDS             12804..13433
                     /gene="coaE"
                     /locus_tag="ACG8FK_001762"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS5097366.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="ACG8FK_001763"
     CDS             13485..13817
                     /locus_tag="ACG8FK_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5097367.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
BASE COUNT         4662 a         2431 c         3038 g         3698 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggtaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaa
//
LOCUS       DAZUMV010000070        13720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000070 DAZUMV010000000
VERSION     DAZUMV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.070"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..901)
                     /locus_tag="ACG8FK_001764"
     CDS             complement(<1..901)
                     /locus_tag="ACG8FK_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS5097368.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(925..969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1184..2152
                     /locus_tag="ACG8FK_001765"
     CDS             1184..2152
                     /locus_tag="ACG8FK_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5097369.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2265..2543
                     /locus_tag="ACG8FK_001766"
     CDS             2265..2543
                     /locus_tag="ACG8FK_001766"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5097370.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2661..2897
                     /gene="secG"
                     /locus_tag="ACG8FK_001767"
     CDS             2661..2897
                     /gene="secG"
                     /locus_tag="ACG8FK_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS5097371.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3000..3755
                     /locus_tag="ACG8FK_001768"
     CDS             3000..3755
                     /locus_tag="ACG8FK_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5097372.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3758..6115
                     /gene="rnr"
                     /locus_tag="ACG8FK_001769"
     CDS             3758..6115
                     /gene="rnr"
                     /locus_tag="ACG8FK_001769"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS5097373.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6137..6601
                     /gene="smpB"
                     /locus_tag="ACG8FK_001770"
     CDS             6137..6601
                     /gene="smpB"
                     /locus_tag="ACG8FK_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS5097374.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6903..7622
                     /gene="atpB"
                     /locus_tag="ACG8FK_001771"
     CDS             6903..7622
                     /gene="atpB"
                     /locus_tag="ACG8FK_001771"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS5097375.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7675..7890
                     /gene="atpE"
                     /locus_tag="ACG8FK_001772"
     CDS             7675..7890
                     /gene="atpE"
                     /locus_tag="ACG8FK_001772"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS5097376.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7981..8505
                     /gene="atpF"
                     /locus_tag="ACG8FK_001773"
     CDS             7981..8505
                     /gene="atpF"
                     /locus_tag="ACG8FK_001773"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS5097377.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8492..9034
                     /locus_tag="ACG8FK_001774"
     CDS             8492..9034
                     /locus_tag="ACG8FK_001774"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS5097378.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9057..10613
                     /gene="atpA"
                     /locus_tag="ACG8FK_001775"
     CDS             9057..10613
                     /gene="atpA"
                     /locus_tag="ACG8FK_001775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS5097379.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10627..11529
                     /locus_tag="ACG8FK_001776"
     CDS             10627..11529
                     /locus_tag="ACG8FK_001776"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS5097380.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11552..12958
                     /gene="atpD"
                     /locus_tag="ACG8FK_001777"
     CDS             11552..12958
                     /gene="atpD"
                     /locus_tag="ACG8FK_001777"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS5097381.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMQSTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12972..13394
                     /locus_tag="ACG8FK_001778"
     CDS             12972..13394
                     /locus_tag="ACG8FK_001778"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS5097382.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4535 a         2452 c         2990 g         3743 t
ORIGIN      
        1 tttcggaaaa aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc
       61 aaaattcata acgatcaacc aatgtttgtc cacgacttat tccctgagtt tccacagcgg
      121 tgaagctttt gaaaccttca cagatggccc cctcgatgaa ataagcaaca gctaacggat
      181 cattgatgac acatcctaaa attctttctt gtttccagtg gaagtcaaaa taaaaacgag
      241 tgatcgcttt taaatactct ccctcttcag gattcatttg acagcagtat tcgaggatag
      301 tcggtgtcaa gacgatttct cttgtcacat ctaacccaac catttctatc gtttgtttga
      361 gattctcgaa tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg
      421 gcgaacagtt accatggctt ttgtaagttc cacccatcga gacaaaacga tccatatgtt
      481 tcccaatatt tgggttgact ttcaaagcaa aagcgatatt ggtcaaagga cccaaagcaa
      541 tgactgatgt atttgtcttt tctttgaaat aatcagccaa aaaatcaacg gcatggagag
      601 gttctgcctg ttttctcaag atcatcggga aatttgtttc ccctaaacca tccataccat
      661 gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt ttccattcct tgataaacag
      721 gaatgtctaa acgatcacag cgttccaggc atttcagcac attctgcgta cctatatctg
      781 cagggacatt cccacaaact acggtaatag caactacttc gatatcaggg gatttgacag
      841 ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca atcaataatt acttttcgca
      901 ttatttccaa tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac
      961 cagtccaaga cgagcgtctt gattataatg cacgaaatta gtatactggc ttttattgcg
     1021 aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa ctttaagtaa atgatacaat
     1081 gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat cctatggatc ggtacattgg
     1141 ataatagata gattatagtt tggcagaaag cggcgggtgt acaatggtta ttttttattt
     1201 cattcttatt ctgataatta tagggatcag ttaccatttt ctttcttatt tttatgcaaa
     1261 gaaattagta agagcgggag tacagagagg atatgactgg tatgaagaca ctggtcatcg
     1321 tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa cgacaaaaat tagaagaaga
     1381 gtgtgaaagc ttttgggagc agggaacacc caaaacaatc aaaagttatg acgggttgaa
     1441 actagctggt cagatgttca tccatccaaa taagcagaat aaatgggtca tttgcgtaca
     1501 tgattaccga agcacaggga aaagagacat gtctcatata ggtaaaagat atgcagagaa
     1561 aggattcaac gttcttgtac ctgatttacg tgcgcacgga gaaagtgaag gagaaatcat
     1621 cggaatggga tggctagatc ggctagattt aattgcctgg atccaactca tcttagatga
     1681 acagccgaat gcgtccatta ttttacacgg cggatcaatg ggagcttcta cgatcatgat
     1741 ggcaagtggt gaaaaacttc catcagcggt caaaggattt attttagaca gcggatatgt
     1801 ttctgtttat gcagaattcc gatatatgtt aagtaaaatc actgtctttc ctaaaaaaat
     1861 ggtcatgaga tatgcgaatc attatgccca gaaatacgcc ggttactcct taaagcaagc
     1921 atcagctacg agacagcttg gcagcaacca tctgccttta ttagtcatcc atggtgaaag
     1981 agatcatttt gtccctacag aagcagcgta cacgattcaa aatgctacgg caggggataa
     2041 agcactgctg cttgttccag aagcagaaca cttggaagca tcgatgaaag atccgaaaac
     2101 ctattggaca gtgatttttt catttattga acaaagaata acgatggatt aattttctct
     2161 tcttttttgt aagtaattcg aggataaata attgtttata gatctggaga ctgctaaaat
     2221 aaggtaggaa taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagaag
     2281 aaaaaaatcg tcttgcttta ttcaatgatg agcaacaaga gattggagaa atgacatggt
     2341 cagatgcggg cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg
     2401 gtcaaaagtt agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga
     2461 aaaagatcat tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc
     2521 aagacgtctt acgtcagtca taagaataac agctgaaagt tttgaacttt cagctgttat
     2581 tccatttata tagagttgtc acggactttt atctatgata aagttaaaga gacgatatct
     2641 tttgggaagg aggctgcgat atgtataatt taattttggg aatcgtgatc gttttatcga
     2701 ttgttatggt cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat
     2761 ttaccggcgg tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca
     2821 tgcagcgatc aactgcagtt atgggagcta tctggatgat cttattattt gtcctagcat
     2881 atttatcttc gaagtagttg aataagcctc tcgcaaaacg gcgggcggct ttttttattg
     2941 caaagagaac gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg
     3001 tgagacgagt gagacaatta ccaaaacccc tttatgcaaa acatggaaag cgagcggttt
     3061 tgctgcttca cgcttactca ggaagtccaa acgatgttcg aatgctcgca cgatttttgg
     3121 aaaaagcaga ttatacagtt tatgcgccgc tttttaaagg acacggaaca atggagccat
     3181 atgatatctt gcaagaaaaa gcagaaagct ggtgggcaga cactaaaaaa gccatccatt
     3241 ttttacagtc tgagcagttt tctgatattg ccgtattagg attatcaatg ggcggcatct
     3301 ttgctgtacg agcactagaa gaagaatcag tgattggcgg cggttttttc tgttcgcctt
     3361 tgtctcctgt aaaaacaaac gtgcctgaaa actttgaaaa atacgtacgt caagtgctga
     3421 agacagcagg taaatcagag aaagaaatca atgaaaaagc agttgcttac cgctcgttag
     3481 ctgaacaaca attaatggat atacaagatc aggcagcaat cgtagaaagt aggttgtcag
     3541 atatccaaca gccgatattt ttagctcaag ctgggaaaga tgaaatgatc gaccctaatg
     3601 gcgtatttga aactgcacgc aagttgagcc gtcagcgagt gacacttcaa tggtatcctg
     3661 aaagtggtca tgtgattaca gttggtacag caagacgcga attagaaaaa gacgtcttgg
     3721 aatttttaga aaaattacct tggaatgagg aataaatatg acaaaagaaa cgataaaaga
     3781 gaaaattctc tttttcatgg aaagcagtcg caaacgcagt ttttctatgg aagaaatcgc
     3841 tgaaggttta gggttccaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca
     3901 aatggaaaga gaacagagta tagttttcaa taaaaaagga aaagtgaaac ttcctttaaa
     3961 accaatcttg atcgaaggaa catttcgagc aaatgaacga ggatttggat ttgtaacgat
     4021 tgatcccgaa gaagatgaca tctatatccc aaaagaagcg actggttttg caatggatgg
     4081 agacaccgta gcaatcgata tcatgaagcg agcaaataca gatatggatc gcggagcaga
     4141 ggggaaagta gtcgagattc gagaccgggc gactactcag ttagcagggg aattcgttgc
     4201 ttattcagaa gaagaaatac aagaaacaga tctttacggc gttgttatcc cgaaagatag
     4261 aaagctcaac caatttaaag tatatgcagc agcagaggga gttcgtccag ttgacggaag
     4321 tattgtgctg atcgaactga cccattaccc tgaaaaaggg tatgcgacta gtttagaagg
     4381 gataatcaag caagtgattg gtcataagaa tgaccctggc atggatatcc tctcaattgt
     4441 cgtggctaat ggtatcccta cgaaatttcc tgatgatgta ttgtctgaag cagatgaagt
     4501 tccagatcaa atcagcgaaa aggatataac tggacgtaga gatttaagaa atcagctcat
     4561 tgttacgatt gatggagaag atgcaaaaga tctagatgat gcagttacgg ttaaaaaatt
     4621 agataacggt aattattttc ttggagtaca cattgcagat gtttcttact atgtgacaca
     4681 aggcagtcaa ttggacagag aagcatatga aagagggaca agtgtatatt tgactgatcg
     4741 agtgattccg atgatcccgc aacgtttatc gaatggtatc tgttcactga atccccatgt
     4801 tccaaggctg accatgagtt gcgaaatgga gattgatcca aatggacaag tagtctctca
     4861 tgatattttc ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca
     4921 aatcttagaa gatcaagacg aagaaacgat gcaacgctat gctcagctcg tacctatgtt
     4981 ccaagaaatg ggtgaattgc atcgaatcct tgaaacgatg cgaatcaaac gtggcgcgat
     5041 ttcttttgag gatcgcgaag cgaaaatcct tgttgatgct gaaggacacc caaatgacat
     5101 ccagctaaga agcagaggaa ttggtgaacg cttgatcgaa tctttcatgc ttgcggccaa
     5161 tgaaacggta gctgaacatt ataataaatt aaatctgccg tttatttacc gaatccatga
     5221 acagccaaaa gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct
     5281 ggtccgagga acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt
     5341 agaagacaaa ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc
     5401 acgctattca gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt
     5461 tacatctcct attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag
     5521 tgaagataaa agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga
     5581 tcatagttct aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa
     5641 gaaagcagaa ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt
     5701 tgtgaaattc ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa
     5761 tgaattgaaa caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag
     5821 aactggtctg acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc
     5881 agatgaacga gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg
     5941 tccaaaagca cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc
     6001 agaaaaaaat ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa
     6061 aaaagggaaa aagccatttt acaaagatgt caaaaagaag aaaaagaaaa aataaaatga
     6121 gttggaggaa acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc
     6181 tcgccatgat tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga
     6241 gatcaagtcg atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa
     6301 cggggaagct ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa
     6361 tcatgatccg ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga
     6421 aaacgaattg aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg
     6481 ctatgccaag gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga
     6541 tttgaaacga aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata
     6601 atttcattat cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt
     6661 tagcatttaa tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt
     6721 gcatggggaa aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa
     6781 aatgttttga attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga
     6841 aaaaaccaga tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg
     6901 gattggatga acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt
     6961 gtataatggt gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa
     7021 atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc
     7081 gaggaatcgt aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat
     7141 ttacattgtt tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct
     7201 tacctaacga aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt
     7261 tggcatttat aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag
     7321 gatatttcgt aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg
     7381 aagaatttac aaatttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg
     7441 aagttttgct aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat
     7501 tagcaattcc attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag
     7561 catttgtctt tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat
     7621 aatactcggt taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat
     7681 tatatcgcag cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt
     7741 caagttatct ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc
     7801 cgtacaacaa tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt
     7861 atcgccttga ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc
     7921 gtttgttact gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct
     7981 atgctgaatc aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt
     8041 gtcagcggct catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc
     8101 agcgatattt tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa
     8161 tctcgcatca actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt
     8221 tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat
     8281 attttgaagg atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc
     8341 actgtagaac gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt
     8401 caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc
     8461 aatcaataca ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag
     8521 acgttacggg aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta
     8581 tcaagatctc gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt
     8641 aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg
     8701 ttatgatggg atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga
     8761 ccttttgttc atgatcgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt
     8821 gggaagtgtc acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa
     8881 tgttgcaaaa acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat
     8941 tattggtggc gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca
     9001 attagaaaaa atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg
     9061 ccatcaaagc agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg
     9121 tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac
     9181 atggtttaga aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa
     9241 tggcgcaaaa cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca
     9301 ttcgtgaagg agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg
     9361 ccttgatcgg tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg
     9421 ttactgacaa agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg
     9481 ttaacgaacc aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg
     9541 gacaacgtga attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgatcgata
     9601 cgatcatcaa ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag
     9661 attctacagt tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa
     9721 tcgttgtgaa tgccggtgcg tctcaaccag caccattgct ttatatcgca ccatatgctg
     9781 gtactgcaat gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg
     9841 atctttcaaa acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag
     9901 gtcgtgaagc ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag
     9961 caaaattaag tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac
    10021 aagctggaga tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa
    10081 tcttcttaga aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat
    10141 ctgtatctcg tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt
    10201 tacgcctaga cttagcaagt taccgtgaat tagaagcatt tacacaattc ggttctgact
    10261 tggatgcagc aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac
    10321 aaaaattgca tgcaccgcta gctgttgaaa aacaagtagt gattttgtat gctttgacac
    10381 atggattctt agatagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg
    10441 aatatctgga taccaatcat tcagatatct ttgaaacaat ccgtacaaca aaagatcttc
    10501 cagaagaaga agcgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa
    10561 aaggaagtaa ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg
    10621 agatgaatgg gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg
    10681 agccaaatca cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct
    10741 tcttcacaga agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact
    10801 gctactcagt taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc
    10861 atgctgctca gccgtgctgt gaagaaaact ggttatatcg tcatcactgc agatggcggt
    10921 ttagtcggtg gatataacag ctcgatattg aaacaaacga tgtctatttt agaagaagac
    10981 caccaatcac ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt
    11041 aaagcacgcg gtatcaatct agcatatgag ctgcgcaatc tatctgatca gccaagcttt
    11101 gatgaagtcc gcaaaatcgt aaatatggca acaactatgt tccaaaacga agtattcgat
    11161 gaattatacg tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa
    11221 aaaatgctgc caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat
    11281 atttttgaac cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt
    11341 ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca
    11401 atgaaaacag caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac
    11461 cgtgcaagac aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca
    11521 ctagaatagt attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat
    11581 cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc
    11641 attagttgtt tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga
    11701 attaggggac ggagtgatcc ggaccattgc gatgcaatca acagatggtt tacaaagagg
    11761 aatggaagtc attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg
    11821 tcgcgtattc aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc
    11881 ggaaagaagc gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga
    11941 aattttggaa acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa
    12001 agtcggacta ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca
    12061 taatatcgcc caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg
    12121 tgaaggtaat gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat
    12181 ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt
    12241 gacgattgcg gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa
    12301 cattttccgt ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc
    12361 tgcggttggt tatcaaccaa cattggcaac agaaatgggt caattacaag aacgtatcac
    12421 atctacgaaa aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta
    12481 cactgacccg gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg
    12541 taaattaaca gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc
    12601 tttagcacct gaaatcgtgg gggaagaaca ttataaagta gcaacagaag tccaacacgt
    12661 attgcaacga tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc
    12721 cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa
    12781 ctttaacgtg gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac
    12841 agtgaaagga ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt
    12901 ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa
    12961 aggggtgccc catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct
    13021 atgatcacca tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc
    13081 ctaaacatgc accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag
    13141 attcagatac ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata
    13201 atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg
    13261 cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata
    13321 cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat
    13381 ctaagcactc ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg
    13441 ttatgacaga agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa
    13501 atttttgtga aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag
    13561 aggttgtaaa atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc
    13621 atattttgcc gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg
    13681 gctctttgtc aaatgatgtt ggtaaagaga aatcttagaa
//
LOCUS       DAZUMV010000071        13446 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000071 DAZUMV010000000
VERSION     DAZUMV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.071"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1147
                     /locus_tag="ACG8FK_001779"
     CDS             <1..1147
                     /locus_tag="ACG8FK_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5097383.1"
                     /translation="AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSK
                     KEAPKTEGQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARK
                     IGQKYDLYASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNG
                     TLYTTQSKFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGR
                     YATDTQYYLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFH
                     RGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGD
                     NVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1496..2128
                     /locus_tag="ACG8FK_001780"
     CDS             1496..2128
                     /locus_tag="ACG8FK_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS5097384.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2716..4155
                     /locus_tag="ACG8FK_001781"
     CDS             2716..4155
                     /locus_tag="ACG8FK_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5097385.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4450..4668
                     /locus_tag="ACG8FK_001782"
                     /pseudo
     CDS             4450..4668
                     /locus_tag="ACG8FK_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            4917..5132
                     /locus_tag="ACG8FK_001783"
     CDS             4917..5132
                     /locus_tag="ACG8FK_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097386.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5349..5605)
                     /locus_tag="ACG8FK_001784"
                     /pseudo
     CDS             complement(5349..5605)
                     /locus_tag="ACG8FK_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5792..6703)
                     /locus_tag="ACG8FK_001785"
     CDS             complement(5792..6703)
                     /locus_tag="ACG8FK_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5097387.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6722..7726)
                     /locus_tag="ACG8FK_001786"
     CDS             complement(6722..7726)
                     /locus_tag="ACG8FK_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS5097388.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7723..9846)
                     /locus_tag="ACG8FK_001787"
     CDS             complement(7723..9846)
                     /locus_tag="ACG8FK_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5097389.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9851..12298)
                     /locus_tag="ACG8FK_001788"
     CDS             complement(9851..12298)
                     /locus_tag="ACG8FK_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5097390.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12285..12674)
                     /locus_tag="ACG8FK_001789"
     CDS             complement(12285..12674)
                     /locus_tag="ACG8FK_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5097391.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12734..13237)
                     /locus_tag="ACG8FK_001790"
     CDS             complement(12734..13237)
                     /locus_tag="ACG8FK_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5097392.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13250..>13446)
                     /locus_tag="ACG8FK_001791"
     CDS             complement(13250..>13446)
                     /locus_tag="ACG8FK_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097393.1"
                     /translation="WFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLV
                     FNASGVKDVLLSLFNRIVGA"
BASE COUNT         4111 a         2652 c         2462 g         4221 t
ORIGIN      
        1 tgccgagtct tcaacaactt cgtcaagtga aagtagtacg atttcaagta ccgaatcttc
       61 aacaacttca agtagtacaa gtgaaataac ttcttcgaaa gtgacaaaac caaaccatat
      121 agaatcttca aaaaaggaag cgccaaaaac ggaaggacaa acaaaaactt tttcgacacc
      181 acagcaaata gaatcaacag aatctttcca atcacaacca atactatctg gtaatgaaga
      241 aattcatttt gacaaaaatg aatctgtaga aaattttatt atgaaaattg gtgagtctgc
      301 gagaaaaatt ggtcagaaat atgatttata tgcttctgtt atgattgcac aagcgatctt
      361 agaatctgcc agtggtcaaa gccagttagc tcaggcaccg aattacaatt tatttgggat
      421 aaaaggaact tataatggaa actttgtcat tatggtaaca aacgaagatt tgggtaatgg
      481 aacgctttat acgacccaat caaaatttag agtgtatgaa aactatgagg aaagctttga
      541 agattatgcc aaattattaa ctaagggaat tagtggaaat aaagattttt atgcaggagc
      601 attaaaagct aatagtaaaa catatcgaga agcaactaaa ttccttacag gacggtatgc
      661 aacagataca caatactatt tgaagctaaa tgaattaatt aaaacctacg atttaaccaa
      721 ctatgataaa gaagttaaag gtccacaaat gagtaagact ggataccaag tgcctattca
      781 taattattca ataaccagtt tgtttggaaa tcgtggaaat gaatttcacc gaggacttga
      841 tatggcagct acacagggtg agccgattca tgcagttaaa gctggtaagg ttgttaaagc
      901 agagtttcac tactcttggg gaaattacgt ggtgatacaa cacgaagatg gatcaaccgc
      961 gttatatgct caccagcaac aatacctagt aaaaactgga gataatgttt tgcaaggaca
     1021 aataattggc tatgtaggaa gtactgggaa tagtactggc ccacaccttc atttggaaat
     1081 ttgcaaagat agtagtcttt ctcagagcat gttagttgat ccgaaaacct tattattcgg
     1141 agactaaaaa aggtaatcaa tctgacaaag taacaagaaa atggttggat tcttagacct
     1201 ttgatcagga tattgagact ggaattgata tctcgattac gatgggtttg acaaataaaa
     1261 caagtccatt atcgaatatc gagtgtatta ttgccgtctt tatgctcata ttttgagcag
     1321 gtttcactag atgaaaacaa tttatctatc ttgaggattt cgtgtacgta tcgttcagtc
     1381 ttgtattgta aattagtcat aaaatcagac taagaaaaat tggaaatatt tttttctgat
     1441 ttattaaaag ttaattgaaa ttatatggta cttctttctt gggagggtag gtgcagtgaa
     1501 ggaaagatat ggtcacctta ctgttgttga ggagattact cataatggat ataaaaaata
     1561 tcgttgccta tgtgactgtg ggaatgaaac gattgtatat cgatcaaact tactatcagg
     1621 tagaaccaaa agttgtggat gtggaaatta taaaaatagg cataaatata aaaatttgac
     1681 aaataaaact tttggtaagt tagttgcaat caagccaact aaactgcgaa gtgaaggaac
     1741 tgttgtttgg gaatgtcaat gtctgtgtgg gagaaaaaag ttagtatcgg caaagcgact
     1801 tctaaaaggt gatgtaaaaa gctgtggatg tttgaaatca aaaaagcatc acattgaaaa
     1861 taaaaaattc ggattactta ctgcaaagta tcctttggac aataactgtt catggaaaac
     1921 aaaatgggtt tgtcattgtg agtgtggcag tatgtgtgag ataagttata gtaacttatt
     1981 ttttggacat actcaaagtt gtggatgttt aaaaaggcaa gaaacaagga catgtgttga
     2041 agggacagta atagagtgtc taacgtcaaa attaccgaaa aataatacaa gtggtgttaa
     2101 gggagtttat cgttctaaag agaaatagat tgcgtatata actttaaaaa aaaagaggta
     2161 ctatttagga tcgtatgatt ctattaagag tgcagctcaa gctagaaaag atgctgaaaa
     2221 taaattgttt caacctttga ttgacaaata tcgaggtaat cattaattat tgtggataat
     2281 aattaacagt gatatataaa catctataat atattattca tactattgat atttagtata
     2341 atcctatgga attgaatatc atagcaatgt atttgaaaag caaattggaa aacaaatagg
     2401 tgcaacacca taaatatgaa aaaattggga aaaagtagaa tggaaatttg cttacgtctc
     2461 acgagatagc tcacactatt aatctgaaga acaatgaagt cgacttttag aaattgctag
     2521 aagaaagtaa agcgaattat cactagttac tatagagtaa gttcaaataa agcatctcca
     2581 acttacatgg ttacatgagg atatcaaatc gaaattactc aagcttccta tggctagagt
     2641 cgcgagaagt tgttgggcag tagacatagg ttatattgtt cttagctaca cgatcattag
     2701 aaaggaaatc ctattatgta tagttatcaa gaagatgcaa aagtccaaaa aaatcgctgg
     2761 tggattttga ttgctatatc tatgtttacc ttcatgtcca cattggatgg cagtattgtc
     2821 aatattgcac tacctaccat ctcagatgat atgggcatac caatgaatca agcggaatgg
     2881 gtagtgtccg tttatctgat gattgtctgt tcttgtttgt tgctgtttgg taaaattggc
     2941 gatagccgtg ggaaaatcaa agtatttcgg attggtacgc tgatctttgt cttgggatcg
     3001 ctgttgtgtg ggttcaatca ttcgctaggt attttattgt tcgcacgggt ggtacaagcc
     3061 attggtgcta gtatgactat ggctacaaat gccggaatca tcacggaagt ctttcccata
     3121 catgaacgtg gtcgggcatt gggagcaata ggtgcgtttg tttcattagg ctcgattgct
     3181 ggaccaggtc ttggcggctt gattttgtcc caattttctt gggcgtacat tttttggatc
     3241 aatgtaccgg tgggaatcgt aacgatgttg attgctgaaa aattcttacc aaaagatatc
     3301 accatgagcg gccatacaat cgatggaggg ggctttgctg ccattgcggc tacgattatc
     3361 agcttttttg gagcaatctt tttggggcaa gaaagtggct ttttccatcc agtccccctt
     3421 gtactgttcg taatttcagt gattgccttt gtcagctttt taaaagttga aaagaaagca
     3481 gcctctccat tgatttcttt cggtattttt aagaacaaga tttttaccat gagcttgatt
     3541 acagccttgt tgattttttc ttccaacttc tttgtcaatg tagtgattcc tttttatctg
     3601 caaaatgctc gcgggttgag tcctagctat gctgggctgt tgatgatggt ctttcctttg
     3661 atgatggtcg tcggggcgcc tttaagcggc tacctgaccg accgggttgg cccggagatt
     3721 ttggtattag taggcttggg cttattgtcg gtaacccagg tcatgtatat gtttttgtca
     3781 gtggactctc ctttgtggct atacattgtc gctactgcca tcatgggtgc cggcaatgcc
     3841 ttgttccagt cccccaataa tacgatggtt atgagctcgg tcactatcga aaacttggga
     3901 ctggcaggct cgatgaattc ctttgcccga aatttgggga tggtcagcgg gatttcgctt
     3961 tctacgacgg tcttatatca agcgatgtct gctaagatgg gccagcaagt aactaactat
     4021 atcgcaaatc ggccggatgt ctttttatat ggaatgaaga ttaccttttt aggcagcttt
     4081 gttttgtgtt ttgttgcctt tttcctgact atctggcgta tcaaaaaaca aggaggtatt
     4141 cagaaaaatg tctgaaaaaa ccactgttga agaatttttg gaggcctgcg tatgggtgag
     4201 tgggatttcc atggatgatg gaagatgaag tgaacttcta aaagccgaaa aactcaagta
     4261 ttatcttcaa aatactagtg ctcaatgaga tgcttgattt gatcacaaga agactgcgaa
     4321 agccgctggc aaccgcaagc taggtatccc accgatggac tacgttttag aggctactgg
     4381 ttatgaatac ggagccaacg ctctcatctg cataagaata catcacccag attatgtaaa
     4441 gattttcgat acttctgtca aaaaatcatt aagatagata aatgatttcc gtctagtcaa
     4501 attggttcag aatgtagaca cacagatggt aagaagtcac tcacaattcg agaatggact
     4561 tgtcctattt gccgtataca tcataatcga aacgtcaacg atagtatcaa tattttgacc
     4621 aagggctatg aatacaagca ttgccttaga aaacatcaag aaccttagga acaacgggga
     4681 tagcttggtt aataagagat accgctgttt aggcttgcaa gtatactctt ttatcaagaa
     4741 ttccgtggct ttagtcatga gaggcttaat actatgaaaa ttttctttag catttttgat
     4801 ttaggttcct tttctaatta taataagatg accactacag taacctactg aaatatataa
     4861 acagccactg tagtaccatt ttcaaagtag tctatgagag gagaatcttc attcttatga
     4921 acgctggaat tgcggatatg aatttaataa aaaaaaccct taatgatttt acttccaatt
     4981 caatatcaaa aggtactggt atcaatttaa gcacaataaa aaagctgaaa agtggtgaaa
     5041 gatcagtgga aaagctgaat cttttagatg ctataaaaat aacggaattt gctatgaaaa
     5101 atggaaaagc tgagattgaa atatggagat aattatcgtc tagtgaatta cttgccactt
     5161 agctgaattt aacgatcctt ggtgcttgaa gggggagatt cttgggaata gagcatactt
     5221 gcaagcctat gtctttacta acaacgatat ctcttattaa ccaagctatc cccgtagttc
     5281 taaggctttt acttttatct aagcaataat cgtattcaaa ggaaattttt gacccttaga
     5341 aagtctcatt ttatcagacg tgagcttagg cattgctgaa ttttgttaat tcggttttag
     5401 gttgtgcttt gagcataata tgaaagtatt ctttatcacg atttcattca tctaaggtaa
     5461 tgtgatgtga ttctgaaatt ctttcaaagg cagtttttgc ataagctgac attacatcat
     5521 caatgacatg tttacaatgt ttcactacta aaataagggg gtaaccaaga agaaaaactg
     5581 aatgattatt tgtatctaat tccatggtat atcaaccatt tcctatatag actgattatg
     5641 acatggatta aaataaaaag gcactatgtc ttaattgtcg gttttcgagt taccgaatgg
     5701 cattcatcac ccacctatag aggatgggcg acttctgcct gaaataagtt acactactta
     5761 taacttctaa taaaattaga ttgagtcatt attatttaac atcaacgatt ttccagtttc
     5821 catttttttg taatactaat ttgtactgaa agtagctagt ggtttttgat acattatcaa
     5881 ggtattttat ctttgtactc actagaaaat tttttccgtt ttgtagataa actggattct
     5941 ctaactccga aaactttaag tcaatattga ttggtcgcat cgcaccattt tcaacataat
     6001 actccaattc tttttcagaa gctgatggat aaagtttaaa aaatgtttct aaaaaatcgg
     6061 tgatttcttc cgttgttttg gcatcaattt ctgaatcgtt ttctacttgt tttgcttcat
     6121 attttgcctt gtctggcttt ccaatcattg ttggcaaact agttactaaa tgattcctgt
     6181 tttcatcttc aaagattgtc actcgatatg cagaagaaat tgattgcgtc ttttttccct
     6241 ctgtaatatg ttgctccact aaaaatgaaa cgacaaattc ttccgagtgt ttttcaacat
     6301 ctagaatttt tacagattga acttcggaac tcgtgggaat atctgctcga accatatctt
     6361 ggattaaagc gagaccgttt tctgtaaggt actgttcgag attactcatt ctcttttcaa
     6421 ttgcttcttt gttatttttc catgaaaaat attctttcgc aaaatcctct gtaaaacttt
     6481 caacgccaga agtattcact accttctctt ttataaccac tttttcatga gttgtatgtt
     6541 gatcaatggc ggtgaaattt ttataaacgc caaaagcaag actacaagac aataaaagcc
     6601 aaagggcaag caccattttt cgatgcttac ccacggatac aactcgttct tttttctttt
     6661 ttgggctctt ttccttttga ttccgttcaa tttttatctt cattcttttt cctcttttct
     6721 cttatttgta tcggccagct ccgataagat gctgccgcca atagttttcc gttagattcg
     6781 tccaccccac tggatctcct gcatgaaaca tgcgattctc ccctgcgtaa atgcccacat
     6841 gggtcacata agtaccggca tcataggttg aatgaaagaa aactaaatca cctggtttgg
     6901 catcttttag atcaatatga gtcatggcat catattgatc ctgagccacc cgaggaagtt
     6961 tcaggtcaac tttggcataa caccattggg tcaagccact acaatcaaac cccgtttctg
     7021 gacttgagcc accaaacaca taggccgttc cttcatactt aaatgcctca tcaaaaattt
     7081 cttggactat tttgtcatca aactgagttg tagttaggta ttgcttcact aataaacagt
     7141 aaaacatatt gccataatta taccgccacc caccattttc cttaatcgcg attgggttgg
     7201 ggtaatcgac tttttgacca cctgattttt cctttgaaaa agattcagct aaagcaaaac
     7261 tatgcttctt cccgtttttc ccgacatgat ctagataccc gccaccatag ttataggcct
     7321 gaataatact atcttgatca caccctagtt ctttggaacg cttaaccaaa gacgcaaaat
     7381 agagacagcc ttgtttgatg gatgcctcgg tacttaatga attaggtggt aatccggctg
     7441 actcagaact ttgcataacg tcttccattg tgccaccaga ttccacttga ataatcgcca
     7501 aaagaattgg aatatactct gttatatccg attctttggc atacttttcc accattgcac
     7561 ggtgtttcaa tacttcttct gagacactag ttccgccagg agaagtgcca gaactaccat
     7621 ttccctcact gtcttcacca aacaacaaac ccacacacat caaaataccc ataaaagaga
     7681 tggtcagaaa agttaatgaa ataacaccca ctttcttcaa gttcattttt tctcacctga
     7741 ccttgatttt ggacgatggt tcattctttt ttgtactttt tgattggtct gctttggcag
     7801 agtggacctc tgtttcactt gttggaaaga atttttctta cttgctgatt ggcttttccc
     7861 ttttgaaatg atttcttgac tagttagttt tcgtttgatt tcgggattca gatttgctct
     7921 cacaatccgc tttggtgctt cttttttcag catcatatct ttctgaggtt gagctgcttt
     7981 atctattgga atattcgccc ttctttttcc ctgacgattt gttgcttcac tcagtatctt
     8041 gcgtttttcg tccattttcg catttcttcg ttgtcgttgt ttttctcgca aatccactcg
     8101 atccgctttt tcttggaaca gtccgcgctt gaattcttct ggtgcttttt tcgacttttc
     8161 gattccttta tggagattgt atttcaaatt cgttggcgta ttccttacct gatcctttac
     8221 ttgtttagtc ttatctttca ccaaagcttg ggtatccaaa actttgccga tttttctccc
     8281 agccaaattc attcgggatt gtttcttatt atttgaaact ggattctcct tagttaagtc
     8341 agatagtatt tcgtgatcgt tcggtaatcg tgaattcttt cgtagagaag aaccggatgg
     8401 cttaaatttg gattttgatt gttcctttgc caacttattt ccgactgctg caccaccacc
     8461 aaaagccaaa gctcgtccca catttcgatt gagttgccgc aattttcgat tcattaacat
     8521 ttgcggtttt cgcatgacgc gtcttcccat tgactggcta tcattactct ggaggttgaa
     8581 catactcata atgtcgccca atttgaaata gataccggca aaggtcacaa tttgcagaaa
     8641 agcaaccatg aaaaatggat aggttgccga aatattgaaa aacatcgtgg agatgctaaa
     8701 tgcagtcgta atcaccaaag taacacccgc tcgcatcata atcgtattga ataaacgaat
     8761 aatcgccttc tttcctaaac tttcataagt cggcaacatg gacaataaga aactaattgg
     8821 cagaaacatc gcaaagataa tgaagagaat ttgcgagaac aacataatcc ctgttagaag
     8881 aaaaacaaag aaggaaatgc caatattgaa caataccaaa aagaccacgg tccctaaacg
     8941 gttcatggtc ttggtaatac tgagattcgc attgtcattg tcttcaatct ctgccttgac
     9001 cgcttcttcc cgatctttgc ctttgttttc atctggactc acagatagaa tcttgttgac
     9061 ccggtcttca ccaatttctt ctatgttgga atcatcaaat tgcagtagta accaaggttg
     9121 ttggacttga atcgaaaaca aactatcccg aatcaaatcc acactatctt ttccttggct
     9181 ttctgaattg ggtacaacga tctcggttcc aagggtaagg gccgcttcac taacatctga
     9241 gctaaagtca ttgatttttg tgatataggt aggcgcataa gcaatgaacg aacccgatag
     9301 tagaaaaatc atcacaaaat taatcacggc tcgaactgct ttcgtggttt ctcgttttaa
     9361 taatccggta tacgccacat acacccccag agctaaaatc atcaataata gaaacccaaa
     9421 ataaaatccg gaggtctgga gtccattttc agtaatacca gccaatgttt ggatattctt
     9481 tccaatactt tcagccgtat ccgaaatgaa atctaatttg taagcctctt gaaccacata
     9541 acccgtggcg ttcgacaaat acaaactcac cgtccaaatg aaattagtaa tggcataaag
     9601 accatacatc acgcttttcc ctaagccatc accccaattc caaggcaacc aatcccaaga
     9661 gctatccaca aagaaatcta gttgatagtt gttcagtgaa tatttggaat atagatttcc
     9721 tgcttcaact gtatcatcaa ctagccctgt cgcctcggct accgtaccaa ctaaactcaa
     9781 tagaactatc agagtgagta tgctaataaa aacgattccc acaatccgaa aaagtttctt
     9841 tttcatcccc tcactccatt ctctcttgaa caggtggacg ggtatcaaag gcatggaaga
     9901 gttcttcaaa gactggatgt acctgaacca cgcctacccg accatacaaa tcctgcatca
     9961 agcattggcc attttccaaa tctcgtaggc gtttttgatt gctttcatct tctcgatcca
    10021 agccaaagaa ttccaaggtt ttcttgattt catgaatatc ggtactgcga aacgcaaact
    10081 tcaacccgat attattcttc aagcgttcat ccagtaaatc atcactattt tgcgtgacaa
    10141 agtaaacccc ggcattcata gcccgcccag cacgaacgag cttattcgat aatgttttcc
    10201 cttgcgctac ttgaagaaaa ctccacgcct catctaagtc tacaattttg aagatttctc
    10261 gattactatg gataaagtcc aaggcaaaag tggaaatgac gattagcatg gaaacactaa
    10321 gcaattccat ggtggtatat tcttcaaaag tggtttctgc atctggtaaa accaaatccg
    10381 ctacttgaat gatatttaac tgcctatcta aactaatcga ctgggtaatc gaaccatctg
    10441 agaaaagcaa atgggcaaaa tcataatcgg taaaggactc gatgtgatcc gcgatatttt
    10501 ccgcaactgg tgagccttct tttcgaagct cattaattac ttgtaataat ccccgttgat
    10561 tgctttgggt aaccgtccga atggccttac gcaacactgg aaacttctcc ccatctcgac
    10621 tagaaattcc tgttaaaaag gtcagaatat caattgctag actttcagaa tccttctttt
    10681 tctccataat cacataagga tcaagtaatc cttgattttt tggcttacta gttaagttga
    10741 gaatattgat ttcatccgca atttctggca aggtttcttt ccagccaccc cgttcccctt
    10801 ttggatcgac aatcacagct tgtccaccaa aaaggacggc gtaataaact aagagattgt
    10861 tactaaagga ctttccgcca ccgagtgaac cgaggaatgc cgctgctagc gcattggtaa
    10921 ctgaatcctt gaccccttgt gaggccaatg ccggtttcag gtaaacattg cgccccgtat
    10981 ctacgttata accaaagtaa atgccttcca actcccctaa catctgagtg gcgccaaaac
    11041 caagtccggc gagaaaatca gacgtaacat actggatata atcattcatg tatcgcttac
    11101 tagaaggtaa gaattcttca tgtagcccca tcatatcgcc aaaaggccgg actagtttta
    11161 cattggtatc atcataaaaa tctagcactt catcacaccg gcgctttagt tcatctactg
    11221 attcggcact tactcgaacc acatagctca gtttgtacat ggattctttg gactgatcca
    11281 aggtagtttc caattcatct acggaatcca atgcatctaa gacattggaa tttgtttcat
    11341 tgtcagattg ataggcatgg ttgtccaagt cttttaattc ctttttctta ttgcgaacgg
    11401 tagcaagtgc ctttttgttc gtcacaatct ccacattcat actagtgtca attgggaatg
    11461 taaattgttg ttgctggtaa tagaaaagtt ctgaggaggg aaactccatt tcccccacaa
    11521 ttgtattaat tgttaagtac gctacatatg attcatgatt ctcatgttcc atgcataagt
    11581 accggggctt ttcttcaatc aaacaacgac tgggacgtaa taagtcgtaa cgtttcacca
    11641 aagtttccga cttcagcttt ttctttggta gctgaaattc atattcttca aatggtgtac
    11701 ccttttcgcc atagatatgt tcaatcaaat acatcagatc actcggtgtg actcgtctga
    11761 ctttaaatct tcgagccaat ttactttcca ttagtttttc aagcttggta tatcggcgaa
    11821 tttcttcatt ggataaggaa acaaaatcgc ccatcaaatg atgattcact ccatagacaa
    11881 attcttgaaa gccggagaag aaagatttct tgagattctt tagattgacc tcttcatctg
    11941 tcgcaatcag tttgaagcca ataaaaaagc gataatcaat ctgactatcg ccaatcatgg
    12001 atactaatgc ctctgtctgt aaatcaattc gttgtttcgc tacttctgtt aatcgcccgg
    12061 taatttcctt ttttgatcgt tcttgcgtgg cacgaatact actttctgtg gcaatttgta
    12121 aggcatgaat cttgccttca cgattttgcg caatcaattg acgaaagtta tcgtggactt
    12181 gatatttctg ttcaggtgac aaaaaggagt aattgtaagg tattaactca tagtaggcaa
    12241 aacattctcc ctcttgatta aagacaagat tattttcaat atacttaatc gggtacatag
    12301 ttcacactcc ttaccagagt aatgggttcg tcccatcgtt tcttctgttc tttgactttc
    12361 tttcctgcga aagtgacctt aaatcgtaag tgatagctaa taaaagatcg taaaaatccc
    12421 aatggtttct taccgtcaaa cgttttttgc gacatgaacc aagtaactgc caccggtata
    12481 cctaagtatt ttaaaaaggc cccctctatc atagatagtg gaggcacatg ggcaaatacg
    12541 atgacaaaca gcaaggataa aacaaaccaa gccatctggt taaaagtcac aggaaaaggc
    12601 agttgaaagt cattaattgc ataaatgacc ttttcaactg cccaaatact ggtgtagctt
    12661 tttattttct tcatcaaatc attcctttaa aaagaaggat agtttagaaa tcaactaccc
    12721 ttcaataatt ttgttaatat ggatactcaa aaacacctcg actcgttacc aaataattgc
    12781 ctccaatctc caagtctcta ccacacgcat caaaatcaat atacgtcttc aagtgttctg
    12841 gtacttctcc aaaatcacca gcttctacaa tcaagaaata tgctacatca ctcatatcat
    12901 cgcaatctgg ataatagata atatcatcca catgctctac catctcctca aaagaattga
    12961 agaaagcatg ttgaatctca gaagcaactt caccaactgg cgttccttca agttcctccg
    13021 ctaagccaca caaccgatta atttcctcaa ttggcgtata ctcatcaatc tcaaagggaa
    13081 gttcataatc atgaattgca tattcctcgt attcgtcatt taacccaatt cgttctttca
    13141 cttcatcaaa atcgacaggt ggggtaaacc acgcccccac caattcacct tcgttatatt
    13201 tcccgaggtt cgcaatataa acacgcatct gttccatcgt gcttccccct tacgcaccga
    13261 caattcggtt aaacaaactg agtaagacat ccttcacccc agaagcatta aagaccaatc
    13321 cgaccgcaat caaagcaatc accaaaaagc caatcagttt ggaaaactcc cgcttaaatc
    13381 ctaaaaacaa accaatgacc acaattgcca gtaagactaa ggattgggca ttggttaaaa
    13441 accatt
//
LOCUS       DAZUMV010000072        13257 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000072 DAZUMV010000000
VERSION     DAZUMV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.072"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1303)
                     /locus_tag="ACG8FK_001792"
     CDS             complement(41..1303)
                     /locus_tag="ACG8FK_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5097394.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1296..2195)
                     /locus_tag="ACG8FK_001793"
     CDS             complement(1296..2195)
                     /locus_tag="ACG8FK_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097395.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2340..4118)
                     /gene="uvrC"
                     /locus_tag="ACG8FK_001794"
     CDS             complement(2340..4118)
                     /gene="uvrC"
                     /locus_tag="ACG8FK_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS5097396.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4300..4614)
                     /gene="trxA"
                     /locus_tag="ACG8FK_001795"
     CDS             complement(4300..4614)
                     /gene="trxA"
                     /locus_tag="ACG8FK_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS5097397.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4701..7061)
                     /locus_tag="ACG8FK_001796"
     CDS             complement(4701..7061)
                     /locus_tag="ACG8FK_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS5097398.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7176..7724)
                     /locus_tag="ACG8FK_001797"
     CDS             complement(7176..7724)
                     /locus_tag="ACG8FK_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFS5097399.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7782..8219)
                     /gene="zapA"
                     /locus_tag="ACG8FK_001798"
     CDS             complement(7782..8219)
                     /gene="zapA"
                     /locus_tag="ACG8FK_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS5097400.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8421..9338
                     /gene="rnhC"
                     /locus_tag="ACG8FK_001799"
     CDS             8421..9338
                     /gene="rnhC"
                     /locus_tag="ACG8FK_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS5097401.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9594..10505)
                     /locus_tag="ACG8FK_001800"
     CDS             complement(9594..10505)
                     /locus_tag="ACG8FK_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5097402.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10908..11486)
                     /locus_tag="ACG8FK_001801"
     CDS             complement(10908..11486)
                     /locus_tag="ACG8FK_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5097403.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11506..12174)
                     /locus_tag="ACG8FK_001802"
     CDS             complement(11506..12174)
                     /locus_tag="ACG8FK_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097404.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12189..>13257)
                     /locus_tag="ACG8FK_001803"
     CDS             complement(12189..>13257)
                     /locus_tag="ACG8FK_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5097405.1"
                     /translation="FLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNRT
                     AVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEGK
                     INVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTFK
                     LTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQI
                     KISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGIT
                     GGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVVA
                     VLGALFSVRTIVKIDPLKAIG"
BASE COUNT         3760 a         2851 c         2189 g         4457 t
ORIGIN      
        1 aacaataaat gtccagcttc cttctatatt tttctatatt ttatgctttt tttcgttcat
       61 ttctcataat tgaaatcgat tgtttcaatg aactccataa tccgccttcg ctatacacga
      121 gtacattcga tttgtacatg tttgcagaca agatcatcaa taagaatgtt acggctacaa
      181 gtaccaacaa ggaaatcaat gctccaccca taccgactgt ctcatttgct aagcgaaccg
      241 gcataatata tgaagataag aatggaatat acgaagtgat ccgaataacg atattgtttg
      301 gatctgaagc tcctagtatc aatccaagca tatatccgcc tagagataga taagtcactg
      361 gcaaaattgc ttttgctgta tcttctgctt tgttgaccaa agagccacat aaagctgcta
      421 aaacagaaaa aatcaaaatg ccaaagatca tgaatagaag agaataccac aagaatggtc
      481 caatcaagtt atccaatgag ataccgtcta atatattttt gaccatttcc atatctttga
      541 attgactgta tcctatacca aaaataatac cgtataatgc catttgcgtt aatgcaacaa
      601 gtaaaacacc tgttagtttt ccgtaaaaat gcttttgtgc agtcgtactg gacaggatca
      661 cttccatgat ccgcgtgcct ttttcagaag cgatctcttg tgcaatgatc tgcgcatacg
      721 tcaaaataat aatgaacaag acaatcgttg ctacataact tacgacatat tggactgtcg
      781 agttatcttc tccaactgtc atttttcctt ttgaatcaaa attgactttt tgtcgagtca
      841 aacttgcttg ttggctcaag ctagctactt cttctggtga aatgcctaat tgacttgctc
      901 gaagcatcga ttgcaagcca gtcagttgtt gttgtatcag tagttgtgta ctttgtccta
      961 atgaattttc gctataaagc atcccattta tcttatcatt ctcagttgag actactaaat
     1021 acgcatcgat tttttcttcc gaaagctctt tttgtgcttt ttcttcagaa tcaactgctt
     1081 tgaactcaaa atcatctgtt ttgacttggc tcatttgctg actgatttgc tcattatcag
     1141 ccaatacgcc gattttattg acatcactat tttgttgagc aaagtttcct gctacataaa
     1201 tgatacccag catgataaaa gggactaata tcatgatcaa gaaagaaatc gacttaacat
     1261 tttttaaata gacatctttg gtaataatcc aaaacttacg catcgatttc acctgctttc
     1321 aatttgaaaa tctcttccaa agttggcggt tgctggttaa acataggaat gtagccgaac
     1381 tgagtcgcac gagtaaagat ttctttccca gcttcaggat cttctaaggt aatctctaac
     1441 gtattgtctt catgaaggat cacttttttc acgccttcga tttcttctac ttcctgttgg
     1501 cttaaaccag attccaagaa taacttcgta cggccaaatg attcacgaat ctcatgtact
     1561 tttccattca aaaccatttt tccgtttcgc aacatgatca aatgatcgca gattttttct
     1621 acattatcca tattgtggct agaaaaaatg acacatgaac catgtttttt caattccaat
     1681 attccatctt ttagcaattc agcatttacc ggatctaacc cgctgaatgg ctcatccaaa
     1741 atgatcaatt taggttcatg gattaacgtg gcgatcagct ggactttttg ctgatttcct
     1801 ttagacaaag attttacttt atccgtcttt ttccctttta cctgaaattt ttccatccaa
     1861 aaatcgattt ttggttcaat ctcttttctt gatttccctc taagttctgc aaaataaagc
     1921 agttggtctt gaatcgttac tttcggataa agtccccgtt cctccggaag ataaccgatg
     1981 acatcataat ctttctcccc aagatgatgg ccattccata acacttctcc ttggtcttgt
     2041 gtcaaaaaat ccaagatcaa acgaaaagtc gttgtcttcc cagccccgtt ttggccaatt
     2101 agccccatga tttttccatc ggggatttgg aaagagacat gatcgacagc tgtgtagttc
     2161 ccaaaagttt taacaagatc cttgacttct aacatttctt cactccttct tcaagaaatc
     2221 acatatatcc ttagacacca gacgtaagcg atttattatt taccaacaaa acgtaaacgc
     2281 tctttttgtt gtacataaaa tgataggaca ctttactttc tttgtaaagt gccctttcct
     2341 cattcctgat gtaatttatc aaatacgttt tgtgcgacat ttttcggcag tccgatttct
     2401 tgtaattctt ctgtcgtggc tgctgtgata tttttcaacg atttgaactc tttaagcagc
     2461 attttttttc gttttggtcc taacccttca atttcatcta atcgggaagc aaaactgttt
     2521 ttactgcgta gctgccgatg gaaagtgatt gcgaatcgat ggacttcatc ttggattcgc
     2581 tggagtaaga aaaactctgg tgaatttcgc tcaagcggta ctacagccaa atctataccg
     2641 aatagcaact catttgtttt atgtttatcg ttttttgcca tgccagcaat aggaatatcg
     2701 attcctaatt gattttctaa gacatctttt gccacatcta cttgtccttt tcctccatcg
     2761 atcaagatca aatcaggtaa aggcaaccct tcgcgaatca ctcgtgaata tctgcgatag
     2821 atgacttctc tcattgatgc ataatcatcc ggtccgttga ctgtttttat tttatatttc
     2881 cgatattctt ttcttgccgg ttttccatca atgaatacga ccatcgcagc cacaggattc
     2941 gtccccatga tattagagtt atcaaaagac tcgatacgta ttggggtcgg gatattcatc
     3001 gcgtttccta aacgttcaac tgctccgatc gtccgttcct gttttctggc aatcagatca
     3061 aaccgttcat tcaaagcaac tgaggcattc ttattcgcca attcaactaa ttttttcttt
     3121 tctcctcgtt ttggttgcag cactttcgta gctaacaaag cttctacact gggcttgtcg
     3181 atcgtatcag gaatcaagac ttctttagga atgaaatgct cattttcctg ataaaactga
     3241 ccgaggtaag tcaagaaatc ttcttctgct tcgttataga aaggaaacat tgaaacatct
     3301 cgttcaatta gctttccttg tcttacgaaa aacacttgga cacacatcca tcctttgtca
     3361 atcgcataac caaatacatc tcgatccagt aaatcagtat ttgtcatttt ttggcgagtc
     3421 atgattgtct caatggcacg gatctggtct cggtattctg cagctttttc aaactccatg
     3481 ttttcagcag ctttttccat tttctgatgg atctctgact cgattttttc gtgtcctccg
     3541 tttaaaaact ttttcacttc atcaacgatt ttcgtataga ctttcggatc tacgtcgaaa
     3601 taataaggac acagacattg tcccaaatga tagtaaagac aaggctcctt cgtctgacta
     3661 ggtttgcatt ttctcagagg aaataatcga tcgaggatct tctttgtctc attcgccgca
     3721 ccgacatcgg gatatggtcc aaaatacagt gctttgtctt ttagtacttt acgagtaatc
     3781 accaaacggg gatatttttc attcgttatc ttaaggaaag gataggtttt atcatccttt
     3841 aacataatgt tgtatttagg atcatttttc tttatcagat tgatctctaa gagcaatgct
     3901 tcgatattcg attctgtaac gatgtattca aagtcttcga tttcactaac aagccgttcc
     3961 gttttcgtat tatggctgcc agtaaaatat gagcggacac gattttttaa tattttggct
     4021 ttgccgacat aaatgatcgt tccattttta tctttcatca agtaacagcc gggctgatca
     4081 ggaagtaaag ctaatttgtt tttgattcgt tcgttcatct ggtactcccc ttcatattca
     4141 attaaagtta ttatagcatg tctgtcaaaa aaacaatcca gcctgacaac gtaattatac
     4201 tcaaaaaata ttaatttgaa taaccttaac tcaaccgcaa aaaaaccgag gacggaactg
     4261 gattcagtcc caccctcagc ttatttttgc tgtaagtttt tataaatatt gatcgatcat
     4321 ttgacgtagt tgttcttttg aatgaacacc aacagctttt tctactactt gaccatcttt
     4381 tttcaacatt aatgttggaa tgctcatgat accgaattgt tgaggagttt ctggattttc
     4441 atccacatcc attttagcaa ttctgaattc ttcttcatca tattcttgtt ccaactgatc
     4501 caaaatcggt gcttgcatac gacatggacc acaccaagtt gcccaaaaat caatcaatac
     4561 aagtccttta tctgtttctt cattaaatgt cttatctgta attacttgtg acatatcaaa
     4621 gacctccatt tttaaaatct atgattagta tagcatttct atacttggat agcgaaccat
     4681 ctgcttacct ttagtaagct ttacttaaat ttaacaattg tcgcaccatt tccgccttgg
     4741 tttgccggtg caaactcaaa gcttttgacg ctccgatggt ttttaagata atcagtaatt
     4801 cccttgcgaa gtgcgcctgt tccttttccg tggacaatag tcacttgagg ataaccagct
     4861 agaatggcag aatccaaata ttgatctacc tcacttagcg cttcttcgta tcgttttcct
     4921 cgaaggtcca attgattcgg cacgtgacta ctagattcag atcgtacagc atggataact
     4981 cgttgtgtcg gctctttttg aggagcaaca ggcgtcagct cgtcttcgga cacattcatt
     5041 tttaatatac cgatttctac ttgccactga ttcccgtttt tacgtaaaag cgtccctctt
     5101 tggccgtaag tcgtaaccaa cacttcgtct cccggtttca acgttttttg ttctttcgcc
     5161 ttcttcaata ctttattttt cttcaaagtt tcttcttgat gtagatttgc taattgggtc
     5221 tttgcatcga tcaactgatg ctctttcaca tttccttgcc caatctgctg ttgcatttta
     5281 cgaatatcag caatgatttt ttctgctttt tcttccgcct ctgacacgac ttcgtttgct
     5341 tttttctttg ctttttccat ttctttttca cgttcttcaa agaaatagct gtaagcttct
     5401 ttcaaatcat cgtgaagttc ttgtgcctca gaaacaaaat gacgcatttc cagatattcg
     5461 gtctctgcca ttttccggcg attctctaaa tctgtgatca tttcgtttaa atcttggctc
     5521 tcatcattca tcaactgctt cgcttcatca ataacttctg tatcaagacc taatcggcta
     5581 gagatttcaa acgcattgct tcgtccagga acgccaatca gcaaacgata cgttggactt
     5641 agtgtatcca catcgaactc catgctggca ttaatcgtat ttgcccgatt gtatccataa
     5701 actttcaatt caggataatg cgttgttgcc atcacataag cagacttttt accaagatca
     5761 tctaagattg cgattgctag agcagcacct tcttgcggat ctgttcctgc accgagctcg
     5821 tcaaataata ccagactttt ttcattgact tttgataaaa tatctacagt gttcgtcata
     5881 tgagaagaaa aggtcgatag actttgctca atcgactgtt cgtctcctat atccgcaaat
     5941 acttcctcaa aaataccgat ttgactttct tcacctgcag gaatcggtaa tcctgcttgc
     6001 cccatcagtt gtaacagacc taaagtcttc aacgtgatcg ttttcccacc tgtgttaggt
     6061 cctgtaatga cgatggcttg atagtctttt ccgattgtga tgtcattaga aactactttt
     6121 tcctgatcga tcagtggatg tcttgcttgt ttcaagacga catgattgtc ttcactgatt
     6181 cccggcacga tggcttttac ttctttacca aatcgtgctt ttgcattcat cagatccatt
     6241 ttaccaatca cataagcatt gtggatgatt tcttgacgat ggggaacaag ttcagcagaa
     6301 agttcactta aaatacgttg aatctcattt cgttcagcga tttggtattg acgcaaacga
     6361 ttattcaaat ccaccacttg ttttggttca ataaataaag tctgtcctga agcactttga
     6421 tcgtggacta caccgccaaa tacaccgcgg tattcttgtt tgactgggat aacgtagcgg
     6481 tcatttcgca tggtgatgat cgcatcactt aaatactttg catttttccc acggacgatc
     6541 ccatctaact gttcacgaac agcttgttca cttcgccgaa tgttctgtct tatgctcttt
     6601 aactcaggag aagcgtcatc agtcacgcgt ccgtcttcat cgattgcttc ctttaaacgg
     6661 cggctcaaaa cagggatcgt gactaactga tctgcccaca tatacaaacg tgcaaattcg
     6721 atttcactgt cagaaagatc atcaatgaaa cgattcaatt cgcttgtcgt agacaatact
     6781 ctggaaactt gtgcaagctc cactccattc aagtctgctc cgatctcgat ccgtttcata
     6841 tggggacgga tattttcaag tttaggtata gggatcccgc ctcgcagacg ttgcactttc
     6901 aatccatcct cagtttcttg aagccagttt gtgatcgttt gtttctcact cactggtacc
     6961 aattctgcca gttcttcttt tccttgtgca gtcactacaa attgtctgac catctgtttg
     7021 actttttcaa attccaatgt ttcaaggatt cggttattca ttttatcacc ttatctttca
     7081 ctatgaaaaa agagctgtgc cggacatacg ctacgcagcc ttcttaaata aactactctg
     7141 tacaaaaaac gaaatatttt ccagcactat tttccttaat tcaaaatatt cgttacccaa
     7201 agctcataca tcttgttggt aagaaatggc gttctctcaa cgataaaacg agcgaaacta
     7261 tgctcgccga attgattttg gaccacatct aatggaacaa aggcaagtaa ctgcaaaatc
     7321 aaaaatatag taacgtaagc catgattagt gacaggatgc ctcctgcaat ccagtctacc
     7381 tgtctcaaga tcggcacata cgtcaatcca tggacaaaaa cccccacaaa tcgggtaagc
     7441 agccagccaa ttagcaaaat aatcagaaaa gcagttcctg cataaaaagc ttcatctagg
     7501 tgaaaagcaa atacctgatc aaaaaatgct aactttgatg ttggggtgac agctgggtat
     7561 ggaatgtata attccaaatg acttgctaat ggtttgtaaa aatgctgggc agcaataaat
     7621 gacaatacat atccaatcgc gaatatgatt tgcaaagcaa acccgcgacg tgctccagaa
     7681 aaaaacgcaa ttagcaataa aaataaaatc agcaaactca gcatgctacg ttcctttctc
     7741 ttctaacttt tttaaatttt tcttattttg ataaaaggat cttaattaga agatttttgt
     7801 ttaatttgtc cttttcgttt ctcgtttaaa atctgttgtg cttccacatg atttttaatg
     7861 gtatgatctg cttgaccatt ttctttcaat acttcccgag cttcgttctc gattgcttca
     7921 atgcgtttca cacgattttc aagctctgta aatttgatca tttttttctt taattctgct
     7981 gtttcttctt ccagctccaa gattctttct tgtttcttca attgatccga aagcgcattg
     8041 acagccatca agattgctgc ttgttcatta tccatttgtg gtgataattg tttcaattct
     8101 gctaactgtt cattgatcag cttcgttacg atatccatat gatgcttggt ttcccgtccg
     8161 ataatagtat atgtttggtt ggcaatcact gctttgtagc gtgttttttc gtgcgccata
     8221 cgaatcattt cctcctattt gatgaaagtt ttatttagtg taccatgaag ttggttcttt
     8281 caccgttctc cacaaatatt tttcacatac ttaactatta tatgataaac tttaaaaaaa
     8341 tgctatgtct cgcaatctct tagaaaacga aaagtcgtga gttgcggtat agttttatgt
     8401 ataaaaacga ggagaatttt atgagcaacg tcattatcaa agcaacaaac gaccaactta
     8461 gtcaaatgaa agcatactat cgctcttatt tgttaaataa acagattcct tatactagct
     8521 ttgctgcaaa aaaagacggg acgaccatta ccgcttatac atccggaaaa gtgatgtttc
     8581 aaggtacgaa tgcagcaata gaagctgata aatgggggga ggcacaggat aaacaagcca
     8641 agccagcaaa aacagcctct tctttgccaa aaaatatcag tcaattatcc gttttaggta
     8701 gtgatgaggt cggaaatggc agctattttg gtccgctaac agtatgtgct gcttatgtga
     8761 aacgtgatca gcttgcagaa ttacgtaaac ttggtgttag agattcaaaa gagttaaaag
     8821 atagccaaat catccagctt gctgacatct taaaaaaaac catcccctat aaactgcttg
     8881 tcttatctcc agaaaaatac aatgagatcc aaccaaaata caatgccgtt cggatgaaag
     8941 tcgctttgca caatcaagcg atccatttgc tgctaaaaaa aatcgagcca gaaaaaccag
     9001 aagcgatttt gatcgatcag tttacgccgg aagcaaattt taaaaaatat gcgcgtttag
     9061 aaaaaaatca attacaagaa aaactgtact tcgttactaa aggagaacaa tatcatttag
     9121 ctgttgctgc tgcttctatc atcagtcgtg ccagcttttt ggaagagtta cacaaagcat
     9181 cagctgaagc tggaatcacc cttccatcag gagctggaac gaaatctgac caagtagcgg
     9241 caaaactatt ggagaaaggc ggcatgccta tgcttgaaaa atatgccaaa cttcattttg
     9301 ccaatacaga aaaagctttg aagctgctaa aaaaataatg gaactgtgac aaaagtcatg
     9361 tcacagtccc ttgttccgaa taaacggtgt tctcatgcca actcctcggt cacaagtcac
     9421 aatagtcatc aaacatgaga agctgttttt tgaaattgct ccttgtgacg cggagttaaa
     9481 cggcaggctc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cgataataag
     9541 cccggccaaa cgaaaaatat gaggagctat ttttgtttgg ctgggcttat ttattactga
     9601 aaataagtgg tcaatgcttg tgtcaaactt gcagcaatct gctgctgata actgtctgtt
     9661 actaattcct ttaaatcact atcattattc atataaccca tctcgattaa aatagaaggt
     9721 tgaagatttt ctcgaagaac ttgatagttc ccagtggaga ttcctcttga atccagaggc
     9781 agatcagtga aggagtcagc aattgtctgt gctaatgttt cctcttgtcc atagtaataa
     9841 tatgtcgtga ttccagttgc ttcattcgca tattccgtag agtctgcgtg taaactgata
     9901 aagacatctg cctttttttc ttcactgtag tcgcatatat ctccaagctg tactaattca
     9961 tctgtagtat gggtcaaata aaccgttgct ccagcatcct ctagcgcctt ttttactttt
    10021 atggctgtac ttaatgttat atctttttct tctgtttgtt catcattgct taatgcccca
    10081 gtatcatttc cgccatgccc aggatctaat acaatcactg catcagatag ctcttccggt
    10141 tttgtttgta tattctttgt gactttggtt ccatcttcga gggctaaata tccagtatag
    10201 ccatcatttg tcttgatctg ataagcattt tttgtttcag aaagaaactc cgccagctct
    10261 ccgccgttta tagttgcaag tggggtagtt agcgagtcat ctgtatatag tgtacggtca
    10321 ttttttccga ttttgattaa tgtctcttgt tctgtatcat caaatgattc ctttgtcttt
    10381 gtaaccgtac tgttcgtact gctgaccgtt tgtccaacta tctttttttg accaaaaaca
    10441 tctatttgaa aacagataat aatgataatg attgcaaata caaaagctcc aattattttt
    10501 ttcattcttg actctcacct cgtagatcca gactaagggt caaaacttaa aatcagctta
    10561 agtacgcaaa aaaggggctg taaaagatat aaccgtagaa aataacatta ctgctaaaat
    10621 gatttttttc atttttggca tatcctgaaa tctccttttg actattcgtg gtgattcatc
    10681 tacccaataa atagtttcct tcattctaca aacaattaat ttcttcgtta ttaaattaaa
    10741 acacttcctg gaacaccgac ataataaata aaaatatacc ctgataatca tgagagaata
    10801 acaaatttgt atttatgaaa aactacctat ataaaaaaaa caagatataa cgatcttcgt
    10861 catatcttgc ttcttcacct aactattttt ctccctatat aacagcttta cgcttctcct
    10921 aatacaccat tcaaatataa atcgatcaat tgcgccacat ttagcggttc ttccacaggt
    10981 cttttcgacc gagtgatgta aggtgctaac actaagaaaa aatgccttgc tgcgatttct
    11041 tttgaaatcc catttcttag cacagattgg tttgctgaaa agatttcttc aaaaggttca
    11101 cgataatttt tcttccatag aaagaaaaga tgatctcttg tttcctttga aagattgctt
    11161 tcaagatcgt gaagcatcag aaacaaatcg accagatgtg tctcaatcaa aaactgcgcc
    11221 atttttgtca aggttacttg aaatcccgtt tcgcttgttt gaacgatttc tctcaaagct
    11281 tttccaacat cttttaagtg gttttctaaa actgccgtat atagtttttc tttatcaccg
    11341 aaataatgat agagattggg ttgggtgatc ccaacttttt gtgcgatctc tcttgttgaa
    11401 gtgccgagat atcccttgga cataaacaag tcagtcgctt ctttcaaaat tttttgctgt
    11461 gtatctttgt tgtatcgatg gaccattttt ccacctgctt tttggttatt ctttttcttt
    11521 caatacacca tcttccatca taaatattct atcgcatttg tcaatcagcc gtgtatcgtg
    11581 agtaaccatg atcgttgctt tatttttgtc ttttgattct tttgccaaga tatcgactac
    11641 ttctcgtgcc ttttctgaat ccaaactagc agttggttca tctgctagaa taatggacgg
    11701 atcattgtac aatgcacgag cgattgcgac cctttgccgt tctccaccag atagttcttc
    11761 tggatacttg ttgatcaact tatccacgcc aagctgctca aataattctt ttgcttcttt
    11821 ttgacgatgt tcatgtttga tcttatctac taataataac tgctttttta ctgtcagaaa
    11881 aggaaccaga ttggaagctt gcaaaataaa gccaatttct ttaaaacgta gtttaacgcg
    11941 ttcactttct ttttgattac tgaattcttg atcattgata aatacagagc cactgctagg
    12001 cgtctgcaat ccgcctgcca aagtcaaaaa agtactttta cctgaaccag aaggaccgat
    12061 gattgcaaca aattcccctt tatcgatact catggttgtt tctttcaatg ctttaactac
    12121 ttggtcgcca tcttggaaac ttttttctac ttctttaaat tctattgctt tcatgctctt
    12181 cctccttctt atccaattgc cttcaacgga tcgattttta cgattgtccg aacagaaaat
    12241 agtgcgccta acacagctac taccagcatc aacaagctaa tgacgccaaa aaacagccaa
    12301 ttgctttgga atggaaccgc atcaggtaga acaagggatg ttcccactgt tcctaataga
    12361 ccaagaagaa taccaacaaa tgataaaatg aatgtttgtg ccactacaga acgcgcaata
    12421 aatcctcctg tgatcccttg tgctttcatt acaccaaaga tattaatctt ttgcatcgtc
    12481 agtacataaa tgaaaatccc gatcacaata gccgcaataa cgatcaaaaa tccgatcata
    12541 aatccaaaag tcaatacttg cgcattatat ccaggtaatt cattgatgaa tttagaaatt
    12601 ttgatctgtt ctaaatcttc aggtaaatta ctgattttac ctctgacaac tattgcgtta
    12661 atccgtgcat tttcacttgt atcttctttt tcgaagcgga tctcttggta agcattgatg
    12721 gttgtataga gaactggtga gacattgaat ttggcatgat ccgtaaagcc agttagttta
    12781 aacgtcttgt cactaccaga taacttaact gtatctccta cggctagacc atattcttct
    12841 ttcaaactaa tatcgcctac tgcctcatca tcgttatcaa aagcttttcc ttcaacaaga
    12901 ttgggcatga tgaattgatt tttatcgata ccgaagaagc taacattgat ttttccttct
    12961 tctgtactgt ctttactagt cacgactcca ggtgtctggg caagatacgc cactttccca
    13021 ccttcgactt cttctgctat cttcttagta atcatggaca tccctagatt tgaatttgca
    13081 tctttcgaaa gcacaatcga atctgcctcc cacttgtcga ctgctgttcg attatcttga
    13141 gctaagccat aagctaaccc agtcaaaaaa aataccaggt aggcgatcaa aaacatcacc
    13201 cctgcaacta acgcataacg taactttgaa tgcataattt catttaatgc taaaaac
//
LOCUS       DAZUMV010000073        13148 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000073 DAZUMV010000000
VERSION     DAZUMV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.073"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..151)
                     /locus_tag="ACG8FK_001804"
     tRNA            complement(79..151)
                     /locus_tag="ACG8FK_001804"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
     gene            532..1101
                     /locus_tag="ACG8FK_001805"
     CDS             532..1101
                     /locus_tag="ACG8FK_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS5097406.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1088..1948
                     /locus_tag="ACG8FK_001806"
     CDS             1088..1948
                     /locus_tag="ACG8FK_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5097407.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2138..2842
                     /gene="yycF"
                     /locus_tag="ACG8FK_001807"
     CDS             2138..2842
                     /gene="yycF"
                     /locus_tag="ACG8FK_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS5097408.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2847..4682
                     /gene="walK"
                     /locus_tag="ACG8FK_001808"
     CDS             2847..4682
                     /gene="walK"
                     /locus_tag="ACG8FK_001808"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS5097409.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4679..5995
                     /gene="yycH"
                     /locus_tag="ACG8FK_001809"
     CDS             4679..5995
                     /gene="yycH"
                     /locus_tag="ACG8FK_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS5097410.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5996..6865
                     /locus_tag="ACG8FK_001810"
     CDS             5996..6865
                     /locus_tag="ACG8FK_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS5097411.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6920..7729
                     /locus_tag="ACG8FK_001811"
     CDS             6920..7729
                     /locus_tag="ACG8FK_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5097412.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7832..8461)
                     /locus_tag="ACG8FK_001812"
     CDS             complement(7832..8461)
                     /locus_tag="ACG8FK_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5097413.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8631..9923
                     /locus_tag="ACG8FK_001813"
     CDS             8631..9923
                     /locus_tag="ACG8FK_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5097414.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9999..10661)
                     /locus_tag="ACG8FK_001814"
     CDS             complement(9999..10661)
                     /locus_tag="ACG8FK_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5097415.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10886..11170
                     /gene="groES"
                     /locus_tag="ACG8FK_001815"
     CDS             10886..11170
                     /gene="groES"
                     /locus_tag="ACG8FK_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS5097416.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11221..12846
                     /gene="groL"
                     /locus_tag="ACG8FK_001816"
     CDS             11221..12846
                     /gene="groL"
                     /locus_tag="ACG8FK_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS5097417.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4371 a         2265 c         2833 g         3679 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
      541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
      601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
      661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
      721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
      781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
      841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
      901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
      961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     1021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     1081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgtgccaga
     1141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     1201 ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt tgccagagga
     1261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     1321 aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata
     1381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     1441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     1501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     1561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     1621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     1681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     1741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     1801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     1861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     1921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     1981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     2041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     2101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     2161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgatgt
     2221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     2281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     2341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     2401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     2461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     2521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     2581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     2641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     2701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     2761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     2821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     2881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     2941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     3001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     3061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     3121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     3181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     3241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     3301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     3361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     3421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     3481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     3541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     3601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     3661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     3721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     3781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     3841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     3901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     3961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     4021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     4081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     4141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     4201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     4261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     4321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     4381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtggttttaa
     4441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     4501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     4561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     4621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     4681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     4741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     4801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     4861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     4921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     4981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     5041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     5101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     5161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     5221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     5281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta
     5341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     5401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     5461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     5521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     5581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     5641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     5701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     5761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     5821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     5881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     5941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     6001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     6061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     6121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     6181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     6241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     6301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     6361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     6421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     6481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     6541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     6601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     6661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     6721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     6781 taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga caaacaatac
     6841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     6901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     6961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
     7021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
     7081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
     7141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
     7201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
     7261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
     7321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
     7381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
     7441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
     7501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
     7561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
     7621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
     7681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
     7741 aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt
     7801 atttttttag catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct
     7861 tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga
     7921 tcgtactgtc gccagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg
     7981 tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt
     8041 tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg
     8101 catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag
     8161 aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg
     8221 acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa
     8281 catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa
     8341 catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca
     8401 atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca
     8461 taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga
     8521 atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag
     8581 ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt
     8641 ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg
     8701 ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct
     8761 tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggattttcta
     8821 tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg
     8881 ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag
     8941 ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga
     9001 caggtgctga agggatacta ggagcagtta caactgggct catcatcgga tggggctatg
     9061 cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt
     9121 ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt
     9181 cagcggtttt tcaacagttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc
     9241 agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg
     9301 ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc
     9361 ccgtcttgca agcgaattca ttagcgaatg cagaaatctt agcctcagga caagagttga
     9421 cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta
     9481 cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga
     9541 aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa
     9601 ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac
     9661 cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt cctttattta
     9721 cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag
     9781 tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat
     9841 ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag
     9901 cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa
     9961 tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca
    10021 gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag
    10081 atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc
    10141 agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa
    10201 aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg
    10261 atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg
    10321 atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca
    10381 gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt
    10441 ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa
    10501 aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca
    10561 gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca
    10621 tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg
    10681 gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat
    10741 aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag
    10801 aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa
    10861 aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga
    10921 agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga
    10981 gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga
    11041 aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac
    11101 agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat
    11161 tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc
    11221 atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat
    11281 aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa
    11341 aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa
    11401 ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca
    11461 aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc
    11521 gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa
    11581 ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa
    11641 gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt
    11701 gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg
    11761 atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa
    11821 tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc
    11881 acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa
    11941 caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt
    12001 gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc
    12061 gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact
    12121 gacgatctag gtttagaatt gaaagatgta acaatcgaaa acttaggaaa tgcttctaaa
    12181 gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc
    12241 gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc
    12301 gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt
    12361 gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct
    12421 acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt
    12481 atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt
    12541 gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca
    12601 gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga
    12661 gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc
    12721 ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat
    12781 aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggtatg
    12841 atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac agataaaaag
    12901 aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga aattgctttt
    12961 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gctgtttata
    13021 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct tttttgtgtg
    13081 taattataat gaaaatagtt atttttaata gttataatat tttatctatt aaaagtaaat
    13141 atccattc
//
LOCUS       DAZUMV010000074        13092 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000074 DAZUMV010000000
VERSION     DAZUMV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.074"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..141)
                     /locus_tag="ACG8FK_001817"
     CDS             complement(<1..141)
                     /locus_tag="ACG8FK_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFS5097418.1"
                     /translation="MILIVKYRVEVLFMVTRIFILDDSLVFEKMLVEILEESRILLSP
                     WKF"
     gene            complement(350..1186)
                     /locus_tag="ACG8FK_001818"
     CDS             complement(350..1186)
                     /locus_tag="ACG8FK_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS5097419.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1197..2126)
                     /locus_tag="ACG8FK_001819"
     CDS             complement(1197..2126)
                     /locus_tag="ACG8FK_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS5097420.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2318..2671
                     /locus_tag="ACG8FK_001820"
     CDS             2318..2671
                     /locus_tag="ACG8FK_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5097421.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2745..3401)
                     /locus_tag="ACG8FK_001821"
     CDS             complement(2745..3401)
                     /locus_tag="ACG8FK_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5097422.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3627..5561
                     /locus_tag="ACG8FK_001822"
     CDS             3627..5561
                     /locus_tag="ACG8FK_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5097423.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5647..6870)
                     /locus_tag="ACG8FK_001823"
     CDS             complement(5647..6870)
                     /locus_tag="ACG8FK_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5097424.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6864..8735)
                     /locus_tag="ACG8FK_001824"
     CDS             complement(6864..8735)
                     /locus_tag="ACG8FK_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS5097425.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(8756..9874)
                     /locus_tag="ACG8FK_001825"
     CDS             complement(8756..9874)
                     /locus_tag="ACG8FK_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5097426.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(9957..10790)
                     /locus_tag="ACG8FK_001826"
     CDS             complement(9957..10790)
                     /locus_tag="ACG8FK_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5097427.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10783..11700)
                     /locus_tag="ACG8FK_001827"
     CDS             complement(10783..11700)
                     /locus_tag="ACG8FK_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5097428.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11927..13051)
                     /locus_tag="ACG8FK_001828"
     CDS             complement(11927..13051)
                     /locus_tag="ACG8FK_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5097429.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         4095 a         2635 c         2163 g         4199 t
ORIGIN      
        1 aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc
       61 aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg
      121 atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg
      181 taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg
      241 ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg
      301 acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac
      361 gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg
      421 ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc
      481 attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg
      541 aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt
      601 tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt
      661 aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct
      721 tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat
      781 aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt
      841 tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg
      901 gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat
      961 aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt
     1021 ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa
     1081 tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt
     1141 acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa
     1201 ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag
     1261 cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag
     1321 accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac
     1381 gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct
     1441 aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca
     1501 gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa
     1561 tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt
     1621 tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct
     1681 ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa
     1741 aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg
     1801 gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga
     1861 actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa
     1921 ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc
     1981 catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct
     2041 aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa
     2101 accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct
     2161 ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc
     2221 ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct
     2281 catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac
     2341 tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa
     2401 ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat
     2461 tccggcactt catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct
     2521 gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt
     2581 catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct
     2641 ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga
     2701 acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt
     2761 tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc
     2821 aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc
     2881 aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg
     2941 ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc
     3001 attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa
     3061 gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc
     3121 aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca
     3181 aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa
     3241 taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt
     3301 gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc
     3361 agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt
     3421 gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca
     3481 ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc
     3541 gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga
     3601 attcacaaag tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc
     3661 attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg
     3721 tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa
     3781 ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg
     3841 ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg
     3901 aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta
     3961 cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt
     4021 tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta
     4081 aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac
     4141 gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta gatgaaccaa
     4201 cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg
     4261 gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct
     4321 atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt
     4381 tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc
     4441 ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc
     4501 aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa
     4561 aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg
     4621 aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc
     4681 gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga
     4741 aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca
     4801 ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag
     4861 aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt
     4921 tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag
     4981 cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac
     5041 caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg
     5101 acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag
     5161 tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag
     5221 agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg
     5281 ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc
     5341 gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga
     5401 tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca
     5461 atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat
     5521 gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa
     5581 accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta
     5641 cctttttcac caataagaag accaattact agtcattctc actaaagctt ctaaataata
     5701 gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga
     5761 ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat
     5821 aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc
     5881 tattaattct aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct
     5941 gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat
     6001 aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata
     6061 acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact
     6121 ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt
     6181 atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg
     6241 agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag
     6301 attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca
     6361 agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat
     6421 tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga
     6481 atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa
     6541 atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa
     6601 caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata
     6661 acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat
     6721 taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa
     6781 ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt
     6841 atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt
     6901 tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct
     6961 aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt
     7021 ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg
     7081 ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata
     7141 cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat gattattcct
     7201 tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct
     7261 aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt
     7321 tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct
     7381 cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg
     7441 gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct
     7501 ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa
     7561 tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat
     7621 gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca
     7681 cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac
     7741 aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat
     7801 gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt
     7861 tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc
     7921 aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg
     7981 cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa
     8041 aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta
     8101 taaatcaaac cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa
     8161 ctgtctcgat accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc
     8221 agtgctgatt ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta
     8281 aaaaattgcc aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata
     8341 atctggcttt tttctcctgt atctaatttt ttccataata attgttcatg atgatataac
     8401 gctaataaca ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa
     8461 taatcgggat gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat
     8521 cgttcatcta attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc
     8581 attcctgcaa ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca
     8641 cttgctcgat aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc
     8701 tgtctggtcc ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc
     8761 aaatagctaa ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat
     8821 agatcagtgg cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga
     8881 tcccatcttc atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga
     8941 tagcagcttc atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa
     9001 gaccgcatgc agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat
     9061 caatgagagg aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt
     9121 ctgctatagc tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata
     9181 aagcccatgc atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta
     9241 accgaatata tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt
     9301 tcctcactag atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg
     9361 gatcaccagt ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa
     9421 tcatccaacc aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc
     9481 gtccagcaag taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact
     9541 gttgataatg tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg
     9601 aaaaatggta cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact
     9661 tttctcgttt tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc
     9721 accagtcgat tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg
     9781 gaattttatt ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa
     9841 tttttcttga ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt
     9901 tatgaatgtg gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac
     9961 ttatttgcag ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag
    10021 accattggga tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca
    10081 cctgtactag aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct
    10141 gaaagcatga ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca
    10201 ctagtaataa tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca
    10261 ttggcaccat cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt
    10321 cgcatcataa acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta
    10381 ttcgataatc ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc
    10441 accatcctag atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt
    10501 gctaatgcat aacccgccat tgtagaactg gctgtagcta aaactgtact gattacagca
    10561 ataacaatcg aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag
    10621 ttttttaata gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta
    10681 ctttgcgttg aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac
    10741 acaataatga cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc
    10801 tccgaattta aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat
    10861 agcagaggct tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac
    10921 gaccaaagct gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa
    10981 attacccaag ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat
    11041 ggggatcgtt aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc
    11101 gtatatctct ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac
    11161 actaagccaa atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca
    11221 ctgtacaccg ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa
    11281 aaaagtcatg aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac
    11341 agttctaaaa aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat
    11401 agctaatagc atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga
    11461 tttaataaac aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa
    11521 tgagtagcca attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt
    11581 cacaataaag aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt
    11641 atctgttgct tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat
    11701 cgttaagtcc tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa
    11761 aattttctgc ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg
    11821 cagcggtagt caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg
    11881 tattttcatt gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact
    11941 ttttctcagc tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca
    12001 gtgcgttggc taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt
    12061 gtggattttt ggcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc
    12121 ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag
    12181 gaaaattcaa agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt
    12241 gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt
    12301 ctttgattaa ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt
    12361 catcaagcaa agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag
    12421 attctggcaa ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga
    12481 tatattcttc ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg
    12541 gttgataata acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac
    12601 tcgtttgttg acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat
    12661 aaaatccata ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg
    12721 ctgaagtgtc tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg
    12781 tgatcatttg tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg
    12841 ctccagtttt gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt
    12901 ttgcgacata tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg
    12961 ttacttcttc gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca
    13021 aagctatcac caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt
    13081 atataaaacg ct
//
LOCUS       DAZUMV010000075        13085 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000075 DAZUMV010000000
VERSION     DAZUMV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.075"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACG8FK_001829"
     CDS             complement(79..2199)
                     /locus_tag="ACG8FK_001829"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5097430.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYENSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACG8FK_001830"
     CDS             2429..3607
                     /locus_tag="ACG8FK_001830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS5097431.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACG8FK_001831"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACG8FK_001831"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS5097432.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG8FK_001832"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG8FK_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS5097433.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACG8FK_001833"
     CDS             complement(4960..6213)
                     /locus_tag="ACG8FK_001833"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5097434.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG8FK_001834"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG8FK_001834"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS5097435.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACG8FK_001835"
     CDS             7790..9136
                     /locus_tag="ACG8FK_001835"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS5097436.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACG8FK_001836"
     CDS             complement(9444..9830)
                     /locus_tag="ACG8FK_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5097437.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACG8FK_001837"
     CDS             complement(10098..10247)
                     /locus_tag="ACG8FK_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097438.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACG8FK_001838"
     CDS             10709..10921
                     /locus_tag="ACG8FK_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097439.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACG8FK_001839"
     CDS             complement(11199..12998)
                     /locus_tag="ACG8FK_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS5097440.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4290 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattct catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaa
//
LOCUS       DAZUMV010000076        12691 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000076 DAZUMV010000000
VERSION     DAZUMV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.076"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..948
                     /gene="licT"
                     /locus_tag="ACG8FK_001840"
     CDS             97..948
                     /gene="licT"
                     /locus_tag="ACG8FK_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5097441.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     EKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            1041..2927
                     /locus_tag="ACG8FK_001841"
                     /pseudo
     CDS             1041..2927
                     /locus_tag="ACG8FK_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(2967..3869)
                     /locus_tag="ACG8FK_001842"
     CDS             complement(2967..3869)
                     /locus_tag="ACG8FK_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5097442.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            4100..5503
                     /locus_tag="ACG8FK_001843"
     CDS             4100..5503
                     /locus_tag="ACG8FK_001843"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5097443.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            5503..6936
                     /locus_tag="ACG8FK_001844"
     CDS             5503..6936
                     /locus_tag="ACG8FK_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5097444.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            7081..8007
                     /gene="trxB"
                     /locus_tag="ACG8FK_001845"
     CDS             7081..8007
                     /gene="trxB"
                     /locus_tag="ACG8FK_001845"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS5097445.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            8221..9948
                     /locus_tag="ACG8FK_001846"
     CDS             8221..9948
                     /locus_tag="ACG8FK_001846"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS5097446.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            9963..10313
                     /locus_tag="ACG8FK_001847"
     CDS             9963..10313
                     /locus_tag="ACG8FK_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS5097447.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(10405..11265)
                     /locus_tag="ACG8FK_001848"
     CDS             complement(10405..11265)
                     /locus_tag="ACG8FK_001848"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS5097448.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            11357..11785
                     /locus_tag="ACG8FK_001849"
     CDS             11357..11785
                     /locus_tag="ACG8FK_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5097449.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            11947..12123
                     /gene="rpsU"
                     /locus_tag="ACG8FK_001850"
     CDS             11947..12123
                     /gene="rpsU"
                     /locus_tag="ACG8FK_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS5097450.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            12150..12596
                     /locus_tag="ACG8FK_001851"
     CDS             12150..12596
                     /locus_tag="ACG8FK_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS5097451.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4164 a         2159 c         2795 g         3573 t
ORIGIN      
        1 taaacctgat gagtctccca tggtttcatg gacggctttt tgggtttttc tttttgtttt
       61 ggatacagcc ctatacatca tggaaaggag caaaacatgc tgattgagaa agtactgaat
      121 aacaacgtcg taataaccag ggatgaaaga aatcaggaaa tgatcgtgat gggaaaaggt
      181 ttggcattca aaaagaaagt gggtgacccg atttttgact catgcatcga aaaaaaattt
      241 cagttgacaa atgacgggct ttcacaacag ttccaagaat tggcacaagc gattcctctt
      301 gagtacttag aaatttcttg tgaaatcatt gaatatacaa aagaggctct acaagtccag
      361 ttgaatgatt ctgtgtatat tgcattgacg gatcatttgt atacggctat cgaacgtcac
      421 aaaatgggaa tatttgtacc aaatgtattg ctgtatgata tcaaacaact atttcctaaa
      481 gagtatgcca tcgggaaaaa aacagtgaag aaaatctcgg ataaatacca catcaagtta
      541 tcagaagatg aagccggatt tatcgcattg catttagtca atgcacaagg cattcaaagc
      601 cagcaagaca atacctacct gtttactata acaatacagg atattctaaa tatcgtccgc
      661 tattactata agattacatt tgatgaagat tcagtccatt tttttcgatt cgtcacacac
      721 ttacgttttt ttctgacacg cgtcctagat ggagaaaaat cagaagtggg agaagtttct
      781 gaggaattgt ttcaggttat tcaaaaaaaa tataaggata cgtttggttg cgttgagaaa
      841 atagcagact ttttagccga gacgttcaac taccagatgt ccacggacga aaaaatgtat
      901 ttaacgatcc atactgcgcg tttagtgcat gagaatcgtt gtaaataaaa aaaggaggat
      961 aaattaacaa gaacgtatga aataagggta gtgacattca gttggctaaa ccttccacta
     1021 ataaattgga gggaatagaa atgggaaaat accaagaatt agcgagaaaa atcgtagaaa
     1081 atgtcggcgg gaaggaaaat atcaatggtt tgacacattg tatcacaagg cttcgtttca
     1141 aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa aaatatggac ggtattgtaa
     1201 cggtaatgcg agcgggaggg cagtatcaag ttgtcattgg gaatcatgtg ccagtcgtgt
     1261 ttgaagaggt tataaaagca ggaaacctta cttttgatga ggctgtttca acagaaaaaa
     1321 tgagaccgtt tgatatgctt attgatggaa tatcgggctg tttccagcca ttccttgcta
     1381 tcctagctgc tggcgggatg attcgtggac tgacagcatt ccttgtcttc ttaggcgcgt
     1441 ttgaccgcgg ttctggcaca tttgttatgt ttgataatat cggtgattct gtattccaat
     1501 ttatgcctgt tattatcggt ttaacagctg cccgtaaatt taaagtaaat gaattcgtag
     1561 ggatgttgct tggcgcagca ttaatgaatc ctagcttgtc gctggaagct ttgagtggtg
     1621 cagcagaagc accactaacg accatatttt ctggcactat ttttgaagca cctatttatc
     1681 aaacagtatt cggcattccc tggattgcgc gtaactacgc ttcttctgtc attcccatta
     1741 tctttattgt gttgttagct agccaaattc agaaaccaat taaaaagtta gtacctgaga
     1801 tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat tacaatgcct ttaggttttt
     1861 tactcgttgg accagtattc acattcgcaa ctgatatttt aatggctgga tttgaaacac
     1921 ttttagcact ttcgccagtt atttatggag caatcgtagg cttcttctgg caaattctgg
     1981 tgatgtttgg tctccattgg gcaatcgtac caatgggatt gatgcaattt tctgtgaatg
     2041 gttggcaaaa tattatgaca cctgttgcag tagtgtcttt tggacaaact gcagcactta
     2101 ccgcactcta ctttaagctt agaaatccaa aagacaaagc aattgcaata ccagccattg
     2161 tttctggtat tgtggggatt acagagccag ctatttatgg gtttacatta ccacgtaaaa
     2221 aaatatttat ttttacctgt gtcggtggtg cgttaggggg cgcgtatagt gggttaatga
     2281 atttaacttc ttggaatcaa ggtggactag gcatctttac aattcccaac tatattagac
     2341 cagatggtga tttaacagat gtgattaatg tattgattgg gatcgccatt gcgatggtgg
     2401 tatcctttac gctgactttt ttcttttgga aggatgaagc aggtgaaaca gatgatatac
     2461 agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc gctccactaa
     2521 atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc ttgatctacc
     2581 ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg ttcccgacga
     2641 aacaggcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat gtcggattgg
     2701 atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga taaagagtga
     2761 aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa ggatacagtt
     2821 tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg gaaaaccaag
     2881 gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg gttaggtgaa
     2941 gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa attgaaaggc
     3001 tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac agaaattaga
     3061 tggactaaat tctgttgata attttctgca aaaacacaac tatataacac gtctagaaga
     3121 taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg ctcgcgtgtc
     3181 gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat actggtcaaa
     3241 gctaaaattg gaacatgatt ttgtttcaat atttctgcgg tacgaaggat cacattgttt
     3301 tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta cgcttcatag
     3361 aatgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt cattttcagc
     3421 atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat tttttttgct
     3481 tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga aagtgtatca
     3541 tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat accgtctgtg
     3601 gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata ggctgtttca
     3661 gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt tgctacacgg
     3721 atgagtgtag aggggtggac ataacatcgc tcagcaatcg cttggatggt catttcgcca
     3781 agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg agaaaaggag
     3841 atttctttga ttttttcacg taataacatg tagatacctt cttcttattt ttttacgtaa
     3901 attataactt ttttgaaaat gatttgtaaa cggatttata ctatataact taaaatagac
     3961 attttttgac aagatattta caaaaatcca ctagaaaaca gtaaattttg taaatgttac
     4021 tggaaagaaa cagatttcta ttgtttgttt tgtttacaaa aaagataatg aaagtgtaaa
     4081 aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg gtggaggcag
     4141 cagttacacc cccgagttga tggaaggatt tatcaaaaga tatgaggaat taccgatacg
     4201 agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg ttagtcagat
     4261 gtctcagcgt atgtgggatg cttctccgta tgaagtcaag atctatgcaa ctctggatcg
     4321 ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg gtcaattaga
     4381 tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc aagaaacaaa
     4441 tggtgctggc gggatattca aagcttttcg taccgttcca gtcgttttgg caatcgttga
     4501 ggacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc caagtgggat
     4561 ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgattggat tatgcaatgt
     4621 gccagtgatg gcgatgatga cagaaccagc aatgcttggc aagaaaaaag aagagctgat
     4681 ctacaagttt gccggtctaa atcatttcca ttggcacaaa gtagcagata ataaaggaaa
     4741 tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gagaatggat taccgaaaaa
     4801 tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaacatga tcccttgtgg
     4861 ctaccaccgc tattactatc gtgaagaaga aatgctggaa catgctttag aagaatatca
     4921 aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctat ttgaattata
     4981 caaagatcca gaattagacc ataaacctga acaattgcaa aaacgaggtg gcgcttatta
     5041 ttcagacgct gcctgtgaaa cgattgcttc aatctatgga aataaagaaa cacagatcgt
     5101 ggtatctaca aagaatgatg gagctgttcc tgacctgccg gcagattgtg tagtagaagt
     5161 cactgcctat gttggcgcac aaggagcgag aaatgtagcg tttggttctc ttcctgctgc
     5221 tgaaagaggc tggttgcaag taatgaagaa tatggaattg ctgacgatcg aagcagctgt
     5281 gactggtgat tatgggacag ctttacaggc gtttacgatc aatccactta tacgatcagg
     5341 aaagacagca aaacggatca tggatgagtt gtttattgca caccagcagt atttaccgca
     5401 attcaaggag acaatcagcc gattaaaaga agaaggaatc gaagttcaag atgaactggc
     5461 aagagaatta tcaggtaata cacatataat ggaggaagta taatgggatt cagaaaagat
     5521 tttttatggg gcggtgctac agcagccaat caatgtgagg gcggttatga tgaaggtgga
     5581 cgcggactag caaacgtaga tgttgtgcca atcggaaaag accgttttcc tgttatcacc
     5641 ggaaagaaaa aaatgtttga atttgatgag gatcattttt atccagcaaa aattgcaatc
     5701 gatatgtatc atcgttataa agaagatatt gctttgtttg cagaaatggg gttcaagact
     5761 tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg gggatgagaa agagccgaat
     5821 gaagctggct tgaaatttta tgaggatgcc tttaaagaat gccaaaagca cggaattgag
     5881 ccacttgtga cgattacgca ttttgattgt ccaatgcatt tgatcaaaga atatggtgcg
     5941 tggcgttcca gagaactcgt tggtttttat gaaaatctgt gtcgagtcat cttcaatcgc
     6001 tataaaggac ttgtcaaata ttggctgaca ttcaatgaga tcaatatgat ccttcatgca
     6061 ccattcatgg gcgcagggct gtattttgaa aaaggagaaa atgaagaaca aatcaaatat
     6121 caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc tcatgaagta
     6181 gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc atacacttgc
     6241 aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt cttcattgat
     6301 gtccagtcac gaggagaata tcccgcttat gcattgaaag aaatggagcg aaatggcatt
     6361 cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag aacatactgt tgattttatc
     6421 tctttttctt attatgcttc ccgagtttcg actaccgaca acgaactctt ggaacagact
     6481 gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc aagcaagtga atgggggtgg
     6541 caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg ttatcaaaaa
     6601 ccattgttta ttgtagaaaa cggcttaggt gcagtcgata cgccagatga aaatggttat
     6661 gtggaagatg attaccgaat cgattacctg gcagcgcata tccaggcgat gaaggatgca
     6721 gtagaactag acggagtcga tctattaggc tatacgactt ggggatgtat tgatttagta
     6781 tcagctggta ccggtgagat gaagaaaagg tacggattca tttatgtcga tcgtgataac
     6841 caaggaaatg gcacattagc ccgttcaaag aaaaaatcat tcaactggta taaaaaagta
     6901 attgattcaa atggagaaaa tctttcgaac gattgatcaa tgatttataa aagtctattg
     6961 tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc agaacaatag
     7021 actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga gaatctacat
     7081 atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc tttatatgct
     7141 tcacgttcaa acttatccgt attgatgatc gaacaaggtg cccctggcgg ccaaatgaac
     7201 aatacggcag aaatcgaaaa ttatcctgga ttcgattcga tcatgggccc cgatctagct
     7261 tataagatgt atgaaagtgt ctcaagattt gggacagaaa atgcttatgg aatcgttcaa
     7321 gatatcaagg atcacggcag ccataaagaa gtcatctgtg aagataaaag ttacgaagga
     7381 aaaacagtca ttatcgcaac aggatgtatc caccgtaaat taggcgtacc tggcgaggaa
     7441 gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg atggtgcgtt tttccgtaac
     7501 aaacgtctag tagtcatcgg gggtggggac tcagcagtag aagaagccat ctatttgaca
     7561 caatttgctt ctgaagtagt gatcgtccac cgtagagatg aattacgtgc acaaaagatc
     7621 atccaagacc gtgcatttgc aaatgaaaaa atttccttta tgtgggatac agtcgttgac
     7681 gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag cacgtaatgt caaaaccggc
     7741 aatgaatttg atttagcagc agacggagta ttcatctatg ttggactgga tccattaact
     7801 gcacctttta gagaagcagg tttgacgaac gaagctggct ggattccaac agacaacgaa
     7861 atgaaaacga atatgccagg cgtttttgcc atcggagatg ttcgtgaaaa agatttgcga
     7921 caaatcacta cagctgttgg tgacggcggt attgctggac aacaagtgta taaatacttg
     7981 gagtctttag aagaaacaac agcataagtt taaaaagagt agaagaacgg gttcaattgt
     8041 tcttttactc ttttttttcg cgtgaaattt actgaatctg atttatgcga acacgtccaa
     8101 caccttttga taaaagaatc ctttcttgat ttaagcgttt tttggtcctc atactttcat
     8161 atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc
     8221 atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta
     8281 aaaaaacaat taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca
     8341 ccgttggaat tcggtactgc aggtatgcgg ggaatcttag gacctgggat caatcgaatg
     8401 aacatctata cggtaagaca agcaacagaa ggactagcac gtttcatgga tacacaaggt
     8461 gaagaaacaa aacgccgagg tgtcgctatc gcttatgact cacgccatca gtcaccagaa
     8521 tttgcaatgg aagcagccaa aacactagca catcacaata tcccgtctta tgtattcgaa
     8581 agtctgcgtc caacaccaga gctttctttt gcagttcgtc atttgaagac gtttgcggga
     8641 atcatggtaa ccgcatctca caacccagcg gcttacaatg gatataaagt ctacggagaa
     8701 gatggcggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc
     8761 agcaatccgc taaaagtcga agtattatct gacgaagaag ctaaacatag cgggttgatc
     8821 acgatcatcg gagaagaagt ggacgctgct tatctagaag aaatcaaagc agtgacgatt
     8881 gatcgtgaat tggttgaaac aatggggaaa gatttgaaac ttgtgtacac gccacttcat
     8941 ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta
     9001 ctagttcccg aacaagctgt ggcagatcca gatttcacta cggtcaaatc gccaaatcca
     9061 gaagaacatt cagcttttga atatgctata cgacttggag aaaaagaagg agctgacttg
     9121 ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag cggttcgttt gccagatggt
     9181 tcttatgaag tattgactgg gaaccaaatc ggagcattgt ttgctcaata tattttggaa
     9241 gcacatcaat caagaggcac tttaccagaa aacgcagcgg tattgaaatc aatcgtttcc
     9301 agcgaattgc ctacagcaat tgcggaacac tacggtgcta ccatgttcaa tgtgttgaca
     9361 gggtttaaat tcattgctga aaaaatccaa caatttgaag aagaccattc acatactttt
     9421 atgtttggat tcgaagaaag ttatggttat ttagttaaac cttttgttcg cgataaagac
     9481 gcgatccaag cattgctttt attagcagaa gtagcagctt actataaaaa acaaggcaaa
     9541 acgttgtatg acggcttgca aacgattttt gaaacctatg gctatttcgc agaaaaaacg
     9601 atttctgtga caatgagcgg acaagaaggt tctgcgaaaa tctctgcttt gatggaaaaa
     9661 ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag ttattcaaac agaagacttt
     9721 aaagaattga cgagaacaga ccgtgaagga aacgtagaaa aactaacaac accgccatcc
     9781 gacgtattga agtatacatt ggaagaaaat ggctggattg ctatccgccc ttcaggaact
     9841 gagccgaaaa tcaagtttta tattggtgta aaaggaagtt ctacagaaga cgcagccgaa
     9901 aaagtagcct cttatgaagc gattatcaaa aagatgacag aagaataaaa aacttgagac
     9961 tgatggaaaa agtggtaaac tttagaagtc agtctttttt caagttggag gtgaatggat
    10021 tgccagacac gcaaaaatca aaagtagaga aagtcagtca attattcaaa atgttgagtg
    10081 atcccactcg tcttaaaatt ctgctatatc tgaaagacgg ggaacaaaat gtgacagcaa
    10141 tcacccaagc agtggaaatg gagcagtctg ctgtatcgca tcaactacgt ttattaagag
    10201 aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat tttgtatagt cttgatgatt
    10261 ctcatgtact ggatattttg aaccagacac tcaaacatgt ggaacatcaa taacgatagg
    10321 tgcaaataga taaacactgg agtgtgacaa cgaagaaatt tcgttgtcac actccagtgt
    10381 tttttaagaa aaagacaaaa atgatcaatc gcttttttct gttgtttcag ttccataata
    10441 actatgatct tctaggtcta aagcattcaa aataagcgaa gccgtttctt caatagataa
    10501 agatgctaca ttgatcacta cacatcctag tttttcataa agatcattcg caaaatcgag
    10561 ttcagctcta attttttcga tatcggaata agcagtatca gggtttaatc cgtaagagcg
    10621 catccgttct ttacggatat tatttaaaat atctgggtta tttgttagtc ccacgatttt
    10681 tttaggatcg atctcccaaa gctgtttggg aatatgtgct tgtgggatca aaggtaaatt
    10741 ggcaactttt aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg aagttcggga
    10801 tacgcctagc aaaacgacat ctgcttctaa aaaaccacga ggatctttcc catcatcgta
    10861 ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca ataaatggag
    10921 ggcaccaggt tgccgaattg gcttgatacc agttaaacgt tcaatttctg atatcggtgg
    10981 cgtcatcaca tcaaaactaa ataattgatg ttcttcacaa aaagaacgtg ttatctcgat
    11041 cagttcttga ttgattaatg tatgcaggac aagtgctttt tcttctaatg catcttgcaa
    11101 tgcctgcctt aatgtttttt catctgtcac gaacgttctg cggaataggt tgaactcaac
    11161 ggtaggatac tgagccatag tagcttgcgc tagttttgtc gcagtttctc cagcagagtc
    11221 ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gtcatggata aaacctcact
    11281 tgcatttttt ttatgtttat tataccatac tctagaatgc gtacttcgta atcattttga
    11341 tttggaggga taagtcatga aaaaacagac attgatcgaa caatcattag aaacattgaa
    11401 aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt tagtgaagcg
    11461 taaccgctac gtatctgcta aagaagtttt tgactatatg aatcaggaat tcaaaggggt
    11521 aagttatgat acgatttacc ggaatctcca tgattttgca gagatcgatt tactggaaga
    11581 aaccgattta aatggagaaa tgaagtttcg ttttcattgc tgtcagggga aaatcggcca
    11641 tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa gaaatccata tgtgtccgat
    11701 gaattatttc gaagaacagc taggtggatg cacgatcgaa gggcatagat tcgagatttt
    11761 cggaagatgt gaaaattgtc aataaggatt taaataaaat aacatttgat aattttaaaa
    11821 aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac aggacagtct
    11881 tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg gagggaggga
    11941 attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc tcttcgtcgc
    12001 ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg tgaattctac
    12061 gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg caaaaaattc
    12121 tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta aacgatgaca
    12181 tgaaagcagc gatgaaagcg aaagataagg aatctttaca agtcatccga atgatcaagt
    12241 catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa gaagaactaa
    12301 cggtgttgtc tcgtgaaatg aaacagcgac gtgattcttt gcatgagttt gaagaagcag
    12361 gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa aaatatatgc
    12421 cggaacaact ttccgacgaa gaaattcgac aacttgttca agaagcaatc actcaaactg
    12481 gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc aattatgcct aaagtcaaag
    12541 gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa tcttaatttt
    12601 atcatttaaa ctgcggacag tgattatccg cagttttttg tgttcgtaaa aaaagaacta
    12661 aaaaacagct agaagaaaga atcttattct t
//
LOCUS       DAZUMV010000077        12383 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000077 DAZUMV010000000
VERSION     DAZUMV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.077"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="ACG8FK_001852"
     CDS             complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="ACG8FK_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS5097452.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1197..3038)
                     /gene="dnaG"
                     /locus_tag="ACG8FK_001853"
     CDS             complement(1197..3038)
                     /gene="dnaG"
                     /locus_tag="ACG8FK_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS5097453.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3220..5535)
                     /locus_tag="ACG8FK_001854"
     CDS             complement(3220..5535)
                     /locus_tag="ACG8FK_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5097454.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5720..6580)
                     /locus_tag="ACG8FK_001855"
     CDS             complement(5720..6580)
                     /locus_tag="ACG8FK_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS5097455.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6639..7271)
                     /locus_tag="ACG8FK_001856"
     CDS             complement(6639..7271)
                     /locus_tag="ACG8FK_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS5097456.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTNLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            7438..8199
                     /locus_tag="ACG8FK_001857"
     CDS             7438..8199
                     /locus_tag="ACG8FK_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5097457.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8225..10816
                     /gene="mprF"
                     /locus_tag="ACG8FK_001858"
     CDS             8225..10816
                     /gene="mprF"
                     /locus_tag="ACG8FK_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5097458.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="ACG8FK_001859"
     CDS             complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="ACG8FK_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5097459.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT         3495 a         2626 c         2192 g         4070 t
ORIGIN      
        1 tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca
       61 cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa
      121 tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc
      181 ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc
      241 ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc
      301 gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc
      361 ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg
      421 gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc
      481 aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg
      541 aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat
      601 ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc
      661 cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac
      721 ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc
      781 ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag
      841 ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt
      901 tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct
      961 gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg
     1021 gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa
     1081 atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc
     1141 ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat
     1201 ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc
     1261 tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca
     1321 gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct
     1381 tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa
     1441 aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa
     1501 aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta
     1561 cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc
     1621 gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc
     1681 tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc
     1741 gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt
     1801 ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt
     1861 tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg
     1921 cgcccatgag ccactaattc ttggaaagat gcttctccat acttacggag atattcatca
     1981 ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc
     2041 aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc
     2101 aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata
     2161 gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg
     2221 aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa
     2281 acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt
     2341 tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa
     2401 ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc
     2461 gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact
     2521 tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct
     2581 aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat
     2641 agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca
     2701 gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt
     2761 tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga
     2821 aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct
     2881 gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta
     2941 ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt
     3001 actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg
     3061 atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa
     3121 ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat
     3181 tgctttttca agtataacac atttctatct cattaagcat caatttgcta ctcttttcag
     3241 taactgattt tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat
     3301 gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg
     3361 gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa
     3421 tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta
     3481 atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac
     3541 cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg
     3601 caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata
     3661 cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc
     3721 tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc
     3781 tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt
     3841 agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc
     3901 tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc
     3961 tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg
     4021 cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc
     4081 agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat
     4141 attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg
     4201 aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc
     4261 aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa
     4321 cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc
     4381 ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc
     4441 gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag
     4501 ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata
     4561 cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg
     4621 gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg
     4681 ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt
     4741 tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa
     4801 caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc
     4861 ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt
     4921 gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa
     4981 gcggacagca taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt
     5041 caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc
     5101 tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat
     5161 ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga
     5221 ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg
     5281 tacaagattt cttgcatgta tttcttgata aattccaata acgatattga cgacaataat
     5341 tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa
     5401 gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga
     5461 tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg
     5521 tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg
     5581 tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa
     5641 cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt
     5701 ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta
     5761 gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg
     5821 cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga
     5881 atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac
     5941 aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt
     6001 cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga
     6061 cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga
     6121 cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa
     6181 caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg
     6241 caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac
     6301 ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa
     6361 caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa
     6421 tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga
     6481 taaatcctga accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt
     6541 accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgca ctccttcttt
     6601 catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag
     6661 gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta
     6721 tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc
     6781 ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct
     6841 actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat
     6901 gcttggacag ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc
     6961 aaatatttga caccttgatc aatactgtca gctggattat caatgatctc agcttctcca
     7021 tgcagacttt ccgagctctg catgagattt gtcccttttc cttttgattc ggtcaatatt
     7081 atcgccttga tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt
     7141 tctatttgat agctttccac tcgttttacc gaacgataag ttcttcctcc taaaaacaac
     7201 accgcaccaa taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt
     7261 tttcgcttca tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta
     7321 tcttcttggc tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct
     7381 gtattttttg acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg
     7441 actgatataa tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat
     7501 gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc
     7561 tttttactgc atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc
     7621 gaacgctact ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa
     7681 gcatctatgt tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa
     7741 aaaatcgatc aggcagattt tcttggattt agtgatggag caaatctagc actggtattt
     7801 gccagcagct tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt
     7861 aaaggcgttc gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta
     7921 ctctctttat ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat
     7981 gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg
     8041 ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa
     8101 gcttcctttg tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc
     8161 ttcaatcgag aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat
     8221 ttatttgtta aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat
     8281 ctttttgatt tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct
     8341 atctatccag caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat
     8401 gctattgatt ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg
     8461 gatgcttgaa caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac
     8521 gatcaacaat attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata
     8581 tggacaaaag aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt
     8641 gatggctgga ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt
     8701 tgctcctttt ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt
     8761 cgtcttttta tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac
     8821 tcaaggacag ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct
     8881 ttctgttggt tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat
     8941 tgctgcttca gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt
     9001 aatgatgatt atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat
     9061 cttgctctat cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt
     9121 caaaaatatc ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac
     9181 agaaattgcg cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact
     9241 ttctgccact atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt
     9301 gaaatttcat tttattatcc aatttcctag tatactattg ggatttcttt taatcattat
     9361 gggcagaggg atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt
     9421 tctagcttta ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc
     9481 tattctgctt ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta
     9541 ttcatgggag tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat
     9601 cgttattggg gtatacaatc tgccagattt ccctcatcgg cgtcaccatt ttatttcctt
     9661 tttcttattt ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag
     9721 tttcatgatc gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc
     9781 ctttaatgaa gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca
     9841 attgatcttc ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt
     9901 tcttctccaa ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa
     9961 gaaagaagat tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta
    10021 tttaccagtt ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta
    10081 cgatttcatt aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg
    10141 gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt
    10201 tgatgtctta caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga
    10261 taattggttg ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta
    10321 tctacaaaga gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc
    10381 aacgatcatg cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga
    10441 ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat
    10501 gaaagaagaa catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac
    10561 ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca
    10621 tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc
    10681 acgttatact ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat
    10741 tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat
    10801 acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga
    10861 gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa
    10921 agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta
    10981 ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact
    11041 gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc
    11101 aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat
    11161 gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag
    11221 aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt
    11281 gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt
    11341 tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta
    11401 cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc
    11461 tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct
    11521 cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt
    11581 tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca
    11641 tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct
    11701 gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct
    11761 tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg
    11821 cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact
    11881 aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt
    11941 cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta
    12001 ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga
    12061 ggatttgcta ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc
    12121 ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt
    12181 tcttttgcca taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct
    12241 caccttttta ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt
    12301 tgtaagattc gaactaagct tattatacgt caatattggt caaagtcaac taagagcact
    12361 cgtctgtctt atacgcgaaa aaa
//
LOCUS       DAZUMV010000078        12304 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000078 DAZUMV010000000
VERSION     DAZUMV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.078"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..322)
                     /locus_tag="ACG8FK_001860"
     CDS             complement(<1..322)
                     /locus_tag="ACG8FK_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5097460.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSL"
     gene            complement(319..972)
                     /locus_tag="ACG8FK_001861"
     CDS             complement(319..972)
                     /locus_tag="ACG8FK_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5097461.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1260..1780
                     /locus_tag="ACG8FK_001862"
                     /pseudo
     CDS             1260..1780
                     /locus_tag="ACG8FK_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1778..2497)
                     /locus_tag="ACG8FK_001863"
                     /pseudo
     CDS             complement(1778..2497)
                     /locus_tag="ACG8FK_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2522..2707)
                     /locus_tag="ACG8FK_001864"
     CDS             complement(2522..2707)
                     /locus_tag="ACG8FK_001864"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFS5097462.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            2934..4207
                     /locus_tag="ACG8FK_001865"
                     /pseudo
     CDS             2934..4207
                     /locus_tag="ACG8FK_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4314..5345)
                     /locus_tag="ACG8FK_001866"
     CDS             complement(4314..5345)
                     /locus_tag="ACG8FK_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097463.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5352..6182)
                     /locus_tag="ACG8FK_001867"
     CDS             complement(5352..6182)
                     /locus_tag="ACG8FK_001867"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5097464.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6179..6994)
                     /locus_tag="ACG8FK_001868"
     CDS             complement(6179..6994)
                     /locus_tag="ACG8FK_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5097465.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6991..7815)
                     /locus_tag="ACG8FK_001869"
     CDS             complement(6991..7815)
                     /locus_tag="ACG8FK_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFS5097466.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7805..8905)
                     /locus_tag="ACG8FK_001870"
     CDS             complement(7805..8905)
                     /locus_tag="ACG8FK_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5097467.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9083..9868
                     /locus_tag="ACG8FK_001871"
     CDS             9083..9868
                     /locus_tag="ACG8FK_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS5097468.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9901..10284
                     /locus_tag="ACG8FK_001872"
     CDS             9901..10284
                     /locus_tag="ACG8FK_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS5097469.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10360..10491
                     /locus_tag="ACG8FK_001873"
     CDS             10360..10491
                     /locus_tag="ACG8FK_001873"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5097470.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10460..10696
                     /locus_tag="ACG8FK_001874"
     CDS             10460..10696
                     /locus_tag="ACG8FK_001874"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5097471.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            10708..10794
                     /locus_tag="ACG8FK_001875"
                     /pseudo
     CDS             10708..10794
                     /locus_tag="ACG8FK_001875"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(10774..11745)
                     /locus_tag="ACG8FK_001876"
     CDS             complement(10774..11745)
                     /locus_tag="ACG8FK_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS5097472.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3695 a         2772 c         2227 g         3610 t
ORIGIN      
        1 tcaaagaatt actatcactt tctaaattaa catttatatt atctttgatt tgtatttcat
       61 tcttattatt tttttctttt atgaatactt ttatttcact actcttggaa taaaactcca
      121 aagtttgttc tatatatttt atttcttttc cattcatatt actagaaatt tcatttgcca
      181 tattatgaat cttttctttt ctcgtctcca aatatgtttt aggaaagata aaaaaaatta
      241 atgaatgaac taatataatt attattccaa gaacagaaaa tatttgtatg aacatttttg
      301 gaaatatctt taattttttc attttctctc caatttatat cctacatttc ttatagtagt
      361 tatacagtct aattgtaatt tttttctaag ttcttttata tatacatcta ttactcgatc
      421 ataaggagtt tcttcgctat ctttccatac ataatcaata atttgcattc ttgttaacac
      481 ttgtccatta ttatccaata aacattttaa tacttctagt tcttttgcat ttacatctat
      541 ttcttcacca tttattttag ctgtatagct attaaaatta accgatagat ccttatattc
      601 aaacttctct aaatgtccat aattcttctt aattaaagaa tctattctag cttttaagac
      661 cggcaatgaa aatggctttt ctacatatcc gtctactaaa ttagtaaatg catcaatttt
      721 atattcttca tcactaaatg cagtcaagat tagaattggt aaattgcttt tctttctaat
      781 ttctttcaac acttctaaac catttataaa aggtatctga atgtctaaga taaccaaatt
      841 tatatcgcta ttaaattttg acaaggcttc tcttccatct ttagcttgaa taacagtata
      901 tccaaattct gaaagatatt cacttattcc ctctcttatc atattatcat cttcaacagt
      961 taatattttc atatatacct ccacttaagc ctatacacta gatattatac agcatattat
     1021 ggctctataa tatctagtgt tggtgacgcc ttctgttgcc aatacttttt ctttttttta
     1081 ataaatgcaa acagacacct tgaaatcctt tagaaaaaag gcttctataa tattttgtgt
     1141 tgttggaaaa tcgaaggcga ttttctacta acactttttc ttcgtttcaa ctcaaaaaga
     1201 gactcacttt tggtaaaatt tagtcgacca aaacatcacc ctaccaaagg agtcgctcta
     1261 tggataatca tactagaaaa ttactcggat taacagataa acatttattt tttgatgaag
     1321 aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa gggagattgg
     1381 atgactcacc ttcccattgt gaacactgtg gatcgattcg tattatccgc aacggttcct
     1441 atacgacacg tactcagatg ttgaaagtca aagaaaagtt gactatttta gaattaaaac
     1501 gtacccgttt tctatgctat gattgtgggc agaccttttc tgcgaaaacg gacttagttg
     1561 atgaacatca tcaactaaca aaggaactga aacaggcgat tctcatagaa ctgtacgaaa
     1621 atcaatcacg taatctgaaa atcagaagaa gtaccgtcgg ctcaagcgat attggaaact
     1681 cttactcaaa gattccacga cattggagcc gctcaaacgg cattatcatc gactgtttaa
     1741 acgaccaatt agtcaaacag aaatcgtcga tgaaccgttg tgcacaatgg tgtcctgaac
     1801 gtacagcgac accttccata tccagagcag tcgccacatc acggggatgg atgccatcca
     1861 aattaaaagc aaccacgcca gtatgatgtt caggatcttg cggtccgtaa atggtgatac
     1921 cgccgatcgc ttgtaattta ggtaaaacgt aggcaaccag ttcttgctca taacgatgaa
     1981 tattctccat accaatctca ttcaagtaat cgattgctcc tgcaatgttc ggagtaccag
     2041 cttcaaattt ccatggcagc tctttccatg aactatcgta aagattgaca aaatctatca
     2101 tttcaccgcc gaattcgact ggctccattt gttctaacaa ttgacgtttg ccatataata
     2161 caccgattcc tgtcggcccg cacattttat gcccgctaaa ggcgaaaaaa tctgcatcca
     2221 acgcttgtac atcaactgtc atatgcggtg ctgcttgggc accgactgga ctatcctcaa
     2281 ctacaacagc ctgttcaacc ctcagccgtt tcaagcacaa caaataacct tctggatctg
     2341 gtttgccgac ctgaagatca tcgatcgtca caaaggaatc aattttttcc ttaatcatcg
     2401 gatgtgcttc aaaaaaatag tctagctccc gcagttcaga attgagacta ctccgatttt
     2461 ccagtgctgc tgatccgctg ctcaaaatgt tcagcatcca ctggtctccc aaccgcttga
     2521 cttatccctt cataaaagga cagttctgct gccgtcccaa caccttgttg atagtctgca
     2581 aaaggaattt tatagccgaa ctgctccagt aaataggctt cagtaaatag tgatgaatac
     2641 tctcgtaaaa catccattca ctgtcaatca tcgtcccatc aaaatcaaaa ataagtgctt
     2701 tggtcatctt ctttccccct tacccaaaac aagcaaaagc tgtgtcctgc tctctctaca
     2761 gtatcagcat aaaaactgcc gtctgaaaag tcaaaggtga aagaaatgtg gcgctataat
     2821 ttctaggact gatagtaaat cattctatca gtccttttga gcataaaaaa acaaaacata
     2881 cagaagctcc gttagaattg aatcaccaca acacaatcaa aggagcgttc tgtatgcctg
     2941 atcttcatct tatcaaacta ttactaggaa tcaaagatgg aaacattcaa atcactcatg
     3001 tcgagaagaa aaaaatacat tcaatcaaca gtatcgttgt ttctgccacg ctcattaaaa
     3061 cgattcagcc ctgtcctcag tgtaaacagg tgaatcaaca tgggctgatc gttaagaacg
     3121 gaaagaaaaa atcgctggtc cttctcaata agtgtgcgaa tcaacggact tatcttgctt
     3181 tggcaaagca acggtacctc tgtcgtggct gtcacaccta ttttacggcg aatacctaca
     3241 ttgttgaaaa aaactgtttt atcgccaaac aagtacatta taaaatattg gaggaattga
     3301 ctgaaaaaca agcaatgacg acgatttcta aacattgcgg cgtttcttgg tcaaccgttt
     3361 cgcgaacact cgcttctctg ctccccatga caaacgtgaa aataaattgg ttgcctcgct
     3421 gttcattggt agatgaattt cgttcattga aaaatcaagt cggtccctat tctttttctt
     3481 gtatggatgg cgatactggg aaattactgg atattcttcc ttcacgtaag aagaaagatc
     3541 tcatttctta ttttatgcaa tttgaacgac gtgcacgttt aaacgtaaaa atactcgtaa
     3601 cagatatgaa cgcctcttat gctttgctga taaaagagtg ctttcctcat gccaaactcg
     3661 ttgtggatcg ctttcacatc gtcaagcacc tcattcgcag ttttaaggat atttgactat
     3721 gagtcatgaa aagcttcgac cgaaacgatc caatacaagc caaacactat cgacaagtga
     3781 aggctttatc tcgacttctg atcaaacgac aggacacgct tgtttatgat aaatggacaa
     3841 aatggcgtaa ttttggttgg gcctatttaa ccgaaagtga agtcgtagaa cgtctacttt
     3901 ctacttctga tgaactacga atagcttatg cttactatca agaaattctt caagcttttt
     3961 atgacaaaga agcggacatc ttctttcagc ttgtaaaaac aatgcccaag tcggttccaa
     4021 gagaactgca tcatataaaa aaagcgttta taaactatga atccggcatt cgtttagcct
     4081 tagagttgcc ttactcaaat ggcaaaatag aaaatctgca tacccacatc aaagcattga
     4141 aacgaatagc ttacgggtta ggatgagaac cagaattttc ttaatcaatc atctgatcac
     4201 ttattaaaca taaaaaaaca tctaaagcag aaaatcaatt ctaccttaga tgttcttata
     4261 tcactctatc agtcctattt gacaaagagc cagaaatgtt tgccgactat acactagctt
     4321 tgttgtgcaa aatgctgagt atcaatttcc acagggattg ctagaatatc tttcagctga
     4381 atgtataagg catcgctaat tttctcttcc cgatcgttca accgatccac taaaatttct
     4441 ttgccctcca gcgtttgaaa aacataccgt tgaatgttgc ctaaaataaa tgattccttc
     4501 catagaaccg gaatcttaac cccctgagga accggttcaa attgaatcag ctccggtctt
     4561 aaataataaa tgcagcgatt gcccttagcg gaaaactcgt gggtatactg attcagctca
     4621 taccattcga aacgattata gttgccgata aattccgcaa caaatcggct ctgaggattc
     4681 tgatacaatt ccttcgaggt ggaaacctga gccacccgcc cagcttccat cacaaaaatc
     4741 cgatcactga ttcgcatcgc ctcttcttgg tcatgagtca caaaaatcat cgtcatccct
     4801 agcgattgct ggatagcgcg caaatcccgc tgcagctgtt ttctaattcg ggcatccaaa
     4861 gcacttaatg gctcatccag cagcaggact ttcggttcag tgaccaatga gcgagccagt
     4921 gccacccgtt gtcgttgacc gccagaaaga aactggacat ttttatctgc ctgctccgtc
     4981 atatccacca atgatagcat ctcaaaaacc ttttcctgaa tcacctcagg actcagcttc
     5041 tttaccttca aaccaaaggc tacattttca aaaacagtca tagttggaaa caatgcatac
     5101 tgttgaaaaa caaagccgat attgcgatcc ttcgtaggct tatcggcaat attctcattg
     5161 tccagcacaa tctcaccgct ctttggttgt tccaatcccg tgatacagcg cagcaacgta
     5221 gactttccgc acccgctggg acctagtaaa gtaaccagct cacccttttc aatctgaatt
     5281 tcatcaatag ctaaaatctg tttttcatca aaaaacagat tgagatcatg tatttctaaa
     5341 aacattttcc gtcacaactc ctttaagggc ttctttttcg actgcttctc ttccctcgtc
     5401 tgcttaaggg acagctgttg cttttgtcga taagtcagca taaaggtagt taaagcaatt
     5461 cccaccataa acagcaaata caaaaccatc acagcgctag ccaaatggcc gttttcgttc
     5521 atccgccgca gcatatagat gcgtaccgtc tgaaatcgac cgccaatcaa caggttcgtc
     5581 agcatgtact ccccaaaaac cgaagaaaaa atcatcagag aaactagggt cacaccgata
     5641 cggatgtttg gaaacagcac ctggataata atcgtagaca tcggggcgcc caaggtttct
     5701 gctgcatcca ctaattcctt cagattaatt aagcgcatct ggttgttaag actaagatag
     5761 acaatcggca acgccgtaat aaacaatgca cctaccaaca ctacaaacat tgggatacca
     5821 gtttcagcat acgttcgaag caaagccgtt accaaaatca ctcctggtaa agcatacggc
     5881 agcaggagca gcttttccag ccaacgattc aaacgaggga aatacaaatg aatccaaata
     5941 atggtgggaa tcatcaacaa cagaatcgtc accaagacca cacctgctaa taaaagtgag
     6001 cgacccaccg cagcaataaa ttccctgtcc gtcagcagct gttggaacca ttgcaaggta
     6061 aaatcactag gtaaaagcga ttttgaccat ttagtggagc tggcatacaa aatcgttcca
     6121 ataatcggca gcaagtaata caggacaaag cagaacaaaa tcggttttat taactttttc
     6181 acgtctgcac caccttgcct ttcttttgaa taatcaacat tgccgttacc agcaggacac
     6241 ctaaagcgac tgccaacgca ctgcctaaat taggcttggc gaacacatct cccgacacta
     6301 aagccgcaat tcgaatcgtt aaaagattta cattactgct ggtcagggca tacgctgttt
     6361 cataggtccc catgccatta gcaaataaga tgacaaaggt gctggcaatc gatggtcgca
     6421 aaaaaggcaa agccaccttg aaccaagcat acgctgatga agcgcccagc aattgtgcgg
     6481 actccagcca ttcctttttc acttccttca tgctgggata caaaaacaag acccctaacg
     6541 gaatttggaa ataggtatac gtgatggtca atccccacca agaatacaaa ttgaagtccg
     6601 ctagaaatgg caaaatcatt ttcagcacac ctacattccc caacaaaata ataaaagaga
     6661 atgccagcgg aatcccagca aaattagctg ccaaattgga aacaaaggtg attttctcct
     6721 gcaatttagt cgatagattc attaagcata aacataaaat aaacgtcccg attaacccaa
     6781 tcacactgga gctgacagca atcagtacgc tgttaacaaa ggcctgtgtg taatatttgt
     6841 tgctgaaaat atcactgtaa ttggccaatg tccactggtt agttaaatca ctgtgcaagc
     6901 tgcacagaac cataaaaagc agcggcagca gtaaaaatgc acagaagata aaggaggcag
     6961 gaaccagaac caagcgtttt ttcatctctt tcatcccctc atcaattctt gaatgtcctg
     7021 cattttctct gacggtgcaa ttcctaataa ccagcaaaac aatggagccg ccagcagctg
     7081 tggtacagtt tgttccttca gcttttggcg tttatttgaa atcaaataaa aaggaacttc
     7141 acgatgtgca gccaagctgc cgccatgcag tccgtgcgca tccatcccgt gatccgcagt
     7201 caccacgact tgataacctt tttgcagcca ttcaggaagg taaatagcca ataatctgtc
     7261 cgcctgatta accgctgctg catattcatg agaatcggcg gtaaatttgt gaccgacatc
     7321 atcaatattc atcgaatgta cgaccaaaaa gtctggctga tactgattca ctaaataatg
     7381 ggcatccgca aataaatgcg aatcagggta agtgtcctca ctataaaaga tgccatgctg
     7441 aatcagtcgc tcctcctgat gctggatgcg atcaaccaaa cgattatagg gggcttgatt
     7501 gtacaactcg ctgtaccagt ggtaagcggc ggctcctgtt tttccgccag cttccttaac
     7561 taattcaaac agcgaccact ctttggatcg ctgagcatgg taattactat agatgccatt
     7621 ttcataggct ggaacgccgg tcatcagtac ttcgtataac ggacgtgaat tgctgggcag
     7681 ctcacttttt accttcaata agcttgcttg ctggtgctca actaaatgat tcatgaaccc
     7741 aagctgggcg acggctgtgt cgtagcgaca gccgtctagt ataactacaa tcagtttgtc
     7801 aagtctactt tgcataggtc agtacctccg cctgccaaag ctctggaagc tctacagagg
     7861 tcttgttcca agcttcattg tcttcaactg gcaccgccgc ttcataagca tcctccggca
     7921 gtaatttttc agctacttct ttaggcagct ctaccttgcg aataggtcgc gcatagccct
     7981 ttgccaaatt gatttgccct tcatcagata aaatatattc ccgtgctagc tttgccgcat
     8041 tgggatgagg ggcattttta ttgattaagg ttgtataccc gctaatgatg gagccgtctg
     8101 ttggcacagt tacctcaaaa cgctcggagt caatttggtc acgataattt agtccattga
     8161 agtcccaaac aactgccaca tcgatttcac ctttttcaag atttgctacg ctggcatcaa
     8221 cggtggacaa acggcctttt tctgccattt ttttgtagta attcagacct ggctgaatat
     8281 ccttttcgtc acctccgttg gcaatcgccg cagccaatac agcaaattgt gcttggttgg
     8341 caatcgtcac atcccccacg ctaacttgat agtcaccctc ttgcagctcc ttccagctag
     8401 tcggtgcttg cttcacattc tctttatcgg tgataaagga aatggttccc gtatagctta
     8461 acaaccaatc cccatcatca tcctttgccc aatccggaat ttcctcccaa tagttggtct
     8521 tataaggtaa ggttaactct ttgctttcag ccaacggacc gaagctgata ccgacatcac
     8581 caatatcggc ggttccattt tttccttctg attcaaactt agccaattct tctgcactgg
     8641 acatatccgt atccacgtga cttaagccgt attctttttc aaggtcgttc caagtgccta
     8701 cccaatttgc ccaagtatcc ggcattccca agctggcaat ctctccttct tccttggctt
     8761 tttctataat cgtatctaaa gagtcgtctg ccgcagccga ttgcttcgtt gcttcacctg
     8821 agccgcagcc tgtcagcagg atacttactg ttgctgcaca aaataaaccg atagtcatgt
     8881 tacgaatttt ttcattcatt ttcattcatt cttctcccct ttcgataatt ccattctaac
     8941 aaagctttgt aatgagagaa tcagtttctt gaaaaggttt catgaacaat gtaaaggttg
     9001 tatatggttt gtaaatttcg acgaattccc gttaaccttc taaagctttt gcggtatgat
     9061 agtagaaaaa aggaggctcc ctatgcagta ttatgatcag catctccaca cgtatttttc
     9121 ctttgattca gaggaaaaat ttgaaaacta tttagcctat cagccggagt ttttcgtttc
     9181 tacggatcac tttgatttaa acaatccatc taccggaaaa gatgacattc cggattatcc
     9241 agcctatata aacaaactgg actctttaaa agaaacctat tccaccaaat ttctgcgagg
     9301 tatcgagatt ggcgttgttc caggacaaga agagcggatc gtggattacc tcgctcagca
     9361 tccttatgat ttaaagctgg taagtattca tcaaaatggc cgctttgatt atatggacga
     9421 tgtggtttta accaaagatc ccattcaaac taccaaggaa tattttgatc agatgttgca
     9481 ggtcttgacg gattttccag aaggagatat tttgactcac tttgattatg gattacgccg
     9541 cttcaccttc acagcggaag aattcgcgga gcattttgag gctcagctgc taacgatttt
     9601 caaaaaggtt gttgaacgag aattggcgat ggagttaaat gccaagagct tcttgaaata
     9661 cggcaacgaa gacctttacc gttatgccgt tcctttgtat caaagtgtcg gcggaaagct
     9721 ctttacctta ggctcggatg ctcatgttgc cgaagattat cagctgggct ttaaaaaaat
     9781 gagtcagctg ttggcagact gtcaggtaga aaagttgcta gtcattcaag gaaaagaacg
     9841 ccgcctgacg ccgctgccga ttttgtgatt cctatataat agaagaaagt ggtgaaaaaa
     9901 atgagcaacc tgtgggacta caatcaagag gcacccatcc attatttaat tgcgcgacat
     9961 tgggatgcgt tgaaaattga ggcggtttgc cgatccttgt tagcagccgt tccaaaacaa
    10021 caactggaaa attttttggt ggctgacagc ttgcagcggg aaaaagttca ggcttatttt
    10081 gcagccttta aagatcagcc tttggaatat ttgcatgcgc agtttcatct tttttatcaa
    10141 gtagccgccc cagatgacta caacgattta cgaggacagc tgcaattgac ctttcaagcg
    10201 gatgaaaccg cctataccgt tttattggga atggctcgcc ttggtgatca ggcgaaggtc
    10261 gagtggcgga ttttcgatat ttgatggcag gatcaaggcg tttcagttgc aaaccttcct
    10321 ctctttcaat tagaaaaacc gcagcaggaa gaagtttgtg tgctggtcac cgatgctggt
    10381 gaaattcgtc tccgcttatt tcctgaccaa gctcccctgg ctgtagccaa ctggcgctcc
    10441 ctagctgagc aaggcgttta tgacaacaca cctttcggct cgtgtcatta aggaatttgt
    10501 tattcaaggt ggcgctttgg atggttctgg tgatgaagct gtttccagct atgagggctt
    10561 tttcgctgat gaagtgcatc gcggacttta tcattttaat ggcgccttgg ccttagggga
    10621 ccacggcccc cacaccaacg gcaaccagtt ttttattgtg caaaacacaa aggcgcaggc
    10681 tgacctgcta atgtaaagca agcatacttg gagcttggag gactaccgga gctggatggt
    10741 cgttatactg tttttggaca ggtataccaa gggttagaaa tcgtagcagc gatcgcagct
    10801 cagccgacaa acagtgaaga tgtaccgatt gagccagtgt tcattcgaca aattaacttc
    10861 gaaaaaacat cgtaaaaaga cactcactga cttcagcaaa agctctattc ttgagctggc
    10921 tgcagctagt gagtgtcttt catttatcca taagataaag cttggattca ataaataaat
    10981 aaatgttttg gttaaagctg tgaccagcaa atgaggaacc gtgcgagcca gttgatctgc
    11041 caatgcaaac atttctttag gtgaaggcgc tggcgtgtct gccaacggat actccagtcc
    11101 caatgcttgg taaccgtgca taccatagca gtggtacggt agtaattcta acggtacgaa
    11161 ccgatcaaga tctgccacaa aacaagcaat cgtcagcagc tcggtcaggt tcatattgat
    11221 acctggaacc acaggaactc gaattcgcac ttcgcagtta ggatactcaa ttaaaaagcg
    11281 acgaatattc ttcaaaattt gttggttatc taagccggtc cattgtttgt ggagcttgct
    11341 ggtaaacatt tttacatcca caaacaatgt atccacatag ggagctacct tttggatttc
    11401 attataagca ccacaaaagc tggtttccag tacggtattg attccaatgt atttcgattt
    11461 ttgcaaaatt tctttagcaa aatccgcctg aaccagtgcc tcgcctccgc taatggtcac
    11521 tccgccatcc gagtgaaagt aaaaaacagc atccttctca atctcatcca ttacctcttc
    11581 agcggtcatg gtctggccaa atcctttttc aatctgcttg ctttgtgatt cgggaatcga
    11641 acaccaggca cagcgtaatg ggcagccctt taaataaaca acggtccgta aaccagcacc
    11701 atcatgtaga gaaccctgtt ctattcttaa tactgttcct tccatataga tttttgaatt
    11761 acacactagt ttaaactaaa acacacaagg aaaaacttac catttaatca taaaattagc
    11821 tcatataaat atactaggat gtaaaataga ctggtcaaaa tctggtcaaa aaagaggact
    11881 aatacaggta gtcctctttt ttgttatttt aatattctaa atcatttttt attggtttat
    11941 tcccttattc gatgtagact caaatcacta tatcactaga tatcaaacgg cgtcatacgg
    12001 aatcaaaaac atcaaatttg acctgttaaa tggtcattga ataacattca aaatcaataa
    12061 aacggagtca gacacggagt caaaaacgct gagagtaaaa aatatataca gccttgtagg
    12121 taaagtacca cagggctgta tattttttat ttcagattac cttaaatgat tccatttact
    12181 ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc tcaataaact
    12241 atttttggat acatggtatc tgaaacagct aaaaccttgt cattacagca aaaaatcacg
    12301 ttat
//
LOCUS       DAZUMV010000079        12274 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000079 DAZUMV010000000
VERSION     DAZUMV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.079"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            248..988
                     /locus_tag="ACG8FK_001877"
     CDS             248..988
                     /locus_tag="ACG8FK_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5097473.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(1080..2267)
                     /locus_tag="ACG8FK_001878"
     CDS             complement(1080..2267)
                     /locus_tag="ACG8FK_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5097474.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            2589..3431
                     /locus_tag="ACG8FK_001879"
     CDS             2589..3431
                     /locus_tag="ACG8FK_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS5097475.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            3428..4378
                     /locus_tag="ACG8FK_001880"
     CDS             3428..4378
                     /locus_tag="ACG8FK_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS5097476.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            4393..4626
                     /locus_tag="ACG8FK_001881"
     CDS             4393..4626
                     /locus_tag="ACG8FK_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5097477.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            4957..5541
                     /locus_tag="ACG8FK_001882"
     CDS             4957..5541
                     /locus_tag="ACG8FK_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5097478.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            5672..6340
                     /locus_tag="ACG8FK_001883"
     CDS             5672..6340
                     /locus_tag="ACG8FK_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFS5097479.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            6351..7766
                     /locus_tag="ACG8FK_001884"
     CDS             6351..7766
                     /locus_tag="ACG8FK_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097480.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            7784..8353
                     /locus_tag="ACG8FK_001885"
     CDS             7784..8353
                     /locus_tag="ACG8FK_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS5097481.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            8463..10217
                     /locus_tag="ACG8FK_001886"
     CDS             8463..10217
                     /locus_tag="ACG8FK_001886"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097482.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            10177..11943
                     /locus_tag="ACG8FK_001887"
     CDS             10177..11943
                     /locus_tag="ACG8FK_001887"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097483.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
BASE COUNT         4248 a         1704 c         2246 g         4076 t
ORIGIN      
        1 tatccacttt aagtataatt tttccatacg ccaataaagt agttttagga gcatgttctc
       61 atttgagaac gctccttttt tatatatttt gaaagctact tactatacgc gtagtttcaa
      121 atagttttgt cattctaatc ttcagcaaca gaaaaaaatc taaattataa aatttagggt
      181 tgactaaaat ataatttttg tataccatat gatcgtgata atcattatca tttagaagga
      241 gaaagttatg ttagaagtga aaaagcttaa tgtttgttat aacgattttt tagcattgaa
      301 cgagatttct ttaaaaatac aagaaggtgc tttaacaggt attattggtc caaatggtgc
      361 tggaaaatct acattgctta aagcaatgtt aaatattatc cctcatcagg gagaggtctc
      421 cattaataat gaagatatca ataaaaaact ggtgaagatt gcatatgtag agcaaaaagc
      481 tgatattgat tttacttttc caataaaagt aaaagaatgt gtatctatgg gaacatatgc
      541 cggaatgaaa gtttttcaaa gaattaaaaa tgcagaatgg caaagagttt caaaggcttt
      601 ggaaaaggtc gatatgggaa aatattctaa tcatcaaata ggagaattat ctggaggaca
      661 atttcaacgt gtacttttag caagatgtct tgctcaaaat gcggatttca tatttttaga
      721 tgaaccattt gtagggatag acttagttag tgaacgaata attatggata cgttaaaaga
      781 attgaaacat cagggaaaaa cagtgctaat tgtgcatcat gatttaagca aggtaaagaa
      841 gtattttgat aatattattc ttttgaatcg aaatctaatt gctcacggaa gtgtagaatc
      901 tgtatttaat gaagagaatt taaaaaaagc ctatggagat actatattta ttggagagac
      961 taactatata gagtcaagta aaaattagcc aaatcaaaaa atattttata acagataatt
     1021 ccgaaaaaag ggtacaacaa ataagcttct taccattagt ttttttgaat gttcaatagc
     1081 taatcagaaa caattatata aggctcgttt ttagtcaaaa tagcatgaat cacgtaacaa
     1141 agcttacgag aaaccgcacc taaggcagtt agatgatgtt ttccttcgga aacttttttc
     1201 tggtaaaacg cactaaaaac aggatcacat tgagaagcta ctaaagccgc cgaaaacaag
     1261 gcttttcgca aatatggaga ccctcgttta ctcataacat tatgcgttgc ctcatattgc
     1321 cctgattgtg tcacggaagc gtctatccca gcgtaagcaa cgagtttttt cgggttagaa
     1381 aatttttgaa tatctccgat ctcaccaaga atagtcgcac cgtttatagg accaattcca
     1441 ggaattgtga gaataacaga atttaaagtg tccattatgt gcttgatttc tctttccgtt
     1501 tcctttattt ggctttcaat gaattttaat tgttcaatca ttgaacgtag ttggaaggtg
     1561 aacgcttctt gagcaaattt tactccaaag gagttagaag ctgcttgctt caattggtcc
     1621 gctttttctg ccttgattcg gttcctgctt aactcagcta agtattgggc caaagtttct
     1681 gaggaaatgt tttctaagtc gatcggggca ctaaacgcca gcaataactc tttagaagct
     1741 tttccgaaaa cgccctgctt ggtaaaaatc gtcgcgtatt ctggaaagac ttgatccaat
     1801 acggctataa tttttctttt gaagtcgctc gctgtaccta ctaaataagt tcggtaacga
     1861 gaaagttgtt tcaaagaaaa aacattttca tcggataaga ccgtttcgac aaatgagcca
     1921 tagcgcatta agtcagcaat caaaacagaa tcaataatat cgttttttcg tttccgtatt
     1981 tctgtacctt tacgccatcc gtctgtttga aggggattca caacgtgaat caaataatct
     2041 gattcgtgaa ggtatgaaaa gatggacagc caatagtgcc ccgtggcttc catccctata
     2101 acaaagtcac tgggtgctcc ggaatagtcg accattcgct caattaatga ctctgctcct
     2161 tcagtggtgt taggaaatga aaaacctttt agtaatacct ttccttcctc gttcatgata
     2221 gaagcaacgt gagtgcgttt accgatatca atgcctaaaa agtacatgta tatctgacct
     2281 ttcaaaaaaa gaattttgga ttgaaatcca ctcaactgaa aagcgtcact accttgtacg
     2341 agatacggag taaagtaagg actttcaaca tctaactcat acgcaatcca cttatcagag
     2401 agaggagcca gtctctcgag tgcgggttca atggcccaag gggggaacgg cttagcctct
     2461 caatccaata aatatattat ctcaaaagaa atgagttaat accaaattaa aataacgatg
     2521 gttcataggg ttatccaatc aaaaccggaa ggttaaaacc ctaactctaa tatacaaggg
     2581 ggaataatat gcttcaaaat tttattaatg gtctgtatga ctttcacttt ttacagaatg
     2641 ctctaattac atctgtagtt atagggattg tttctggaac agtaggttgt tttattattt
     2701 taagaggaat gtctcttatg ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa
     2761 tttcttatat tttaggtatt aattttttta ttggtgcgat tgtatttggt ctattagctt
     2821 ctatcattat tacttttata aaaagcaata gtgtaataaa aggagataca gcaattggta
     2881 ttactttcag ttctttttta gcattaggag ttatccttat aggagttgca aatagctcaa
     2941 cggatttatt tcatatctta tttggaaata ttttagctgt acaagatatt gataagtgga
     3001 ttacaattgg cgtttctgtt ttagtgttgg ttacagttgt tctatttttt aaagaattat
     3061 taatcacttc ttttgatcca ttaatggcaa aagcaatggg gatgaatgtc agtttttatc
     3121 attatctatt aatgattcta ttaactttag tagcagtaac agctatgcaa agtgtgggaa
     3181 ctatattaat tgttgcaatg ttaattacac cagctgctac agcatattta tatgcaaaca
     3241 gcttaaaaac tatgatattt atttcggcga cagtcggagc gtgctcatca ttgctaggtt
     3301 tgttcattgg ttatagtttt aacatagcag caggatcaag tattgtttta acagcggcat
     3361 tcttatttgt aattagtttt ctaatctcac caaaacaaaa atttttaaga aagaaggaga
     3421 gaaagttatg atgagaaaat ggaaagcagt actgggaagt ctggggattt tgattgctct
     3481 ttttatattc ggggcatgtt caacaaatag taaagacaaa gatacagtgg cttcgaacga
     3541 aaaattaaag gtagtagtta ctaattcgat tttagcagat attactgaaa atatagcaaa
     3601 agataaaatt gatttacaca gtatcgtacc tattgggaaa gatccccacg aatatgaacc
     3661 tttgcctgaa gatgttcaaa aaacttcaaa agcagatttg attttttata acggtgttaa
     3721 cttggaaact ggaggaaatg cttggtttac aaaattagta aaaaatgcga acaaagagga
     3781 aaacaaagac tattttgcag caagtgatgg catagatgtt atttacttag agggtcagag
     3841 tgagaaaggg aaggaagatc cccatgcttg gttaaattta gaaaacggta ttatttacgc
     3901 taaaaatatt gaaaaacagt tagcggaaaa agatcctgat aataaaaaat tctataaaga
     3961 aaatctagat aagtatattg aaaaattgga ttctctagac aaagaagcta aatctaaatt
     4021 tgcttcaatt ccgaatgata aaaaaatgat tgttacaagt gaaggatgct ttaaatattt
     4081 ctcgaaagcg tataatgtgc cttctgctta catttgggaa atcaacactg aagaagaagg
     4141 aacaccagat caaataaaac acttagttga aaaattacgc acaacaaaag ttccctcctt
     4201 attcgtagaa agcagtgtgg acgatagacc aatgaaaaca gtatcaaaag ataccaatat
     4261 tcctatctat tcaacgattt ttactgattc aattgcagaa aaaggacaag atggtgatag
     4321 ttactatgcg atgatgaaat ggaacctgga taaaattgct gaaggccttt cgaaataatt
     4381 tggagggtag gtatggtaca aattaaaacg actactatac taatttattt actagcaatt
     4441 caggaattat caaaaagaaa aaagaaatta aaaaataatg atttagcaaa agttctaagt
     4501 gttagtccag catctgtaag tgagatgctg gctaaactta gatatgagga ctatttagat
     4561 ataggatttc aattaactga taaagggaaa aaatttattg acgattacaa aaaaggtttt
     4621 aagtaaaaga ctttattttt agggaggata agaaaatggc tattggagtg attatgatag
     4681 gacttaccct aatagctgga tataattttt ttaaatcttt ctcggattat tttagaaaag
     4741 gtgaatgatt tagatgagtc tattaattct tgtaggattg ctttttgtat cagttatttt
     4801 gctctttctc attagtttag ctctaaggaa tgagttcagt aatagacgtt aacgattgaa
     4861 acttcaagtc ctgtgtaaga taatatagtt aattcatctt attaggagga agtacccata
     4921 gaaaccagag attatattac aactcaccaa aaattaatag aaagtggact aagccatttt
     4981 gaaaaagaag gatttcagca aactaagata aggcaactgt gcttgaccgc aggcgtaact
     5041 acgggtgctt tttataaaca ttttacttct aaggaagcgt tatttgaaga aattattcag
     5101 ccgcatatta atgcgcttat taatatgtat aatgaagagt ttaccaaata cctcttaaac
     5161 ttagaaaata tagatataga gaaaagttgg agagagaact tcaatgaatt agatccattt
     5221 attaattata tttttgagaa taaacgtata ttcgatttgg ttttattcca atcagctgga
     5281 accaaatatc aagatattgt tgaaaaaatt acatcatttg tgacaaaaaa gacagtctgt
     5341 tgtctagata agttaaagac tatcggaatg atcaatcaaa atttagtctt taatgaagaa
     5401 gaaatccact tctacattta ctcattctac gctacattct atgatatatt gaagcatagc
     5461 tattcaaaag aaaagactat gctattaacc agaagattgt atcaattttc tgttcctggg
     5521 tggattgaat tactgagtta attaaaaaaa agcttgcatt gagaattgtt ctcagttatg
     5581 ctttttgata agatcgcact gttatctgaa taacggtgca tttttgtcag cttaagataa
     5641 cagtgcgatc ttattttttg gaggtgaaag agtgtcaaaa atcaatccgt taaccttgat
     5701 tactttaaat attttttttc cggtcattat ttttttagga aagggaaata tatttcaagt
     5761 ggggtgtttc attattgcta cagttttagt attgatgttt cgtaaaataa agacatgtat
     5821 ttatttcatc ttgatttatt cagttctgtt tttagtaatg aaaattattg atgctatcaa
     5881 gatagagttt tttactatat tttttgggac atccttatat attgtatttc gaatgttgcc
     5941 ggtcgtaatg atctcatggt tgttggttag tgtctatact agcaatgaat tattatcctc
     6001 tttagaaaaa atacatttac cgaaaaaaat aatgttaagt attacagtag tgatcagatt
     6061 ctttccgact tatcgttctg aaataagaat gattaaagaa tctttaaaaa tgagaaacat
     6121 tcgcttgtct ttttttcagc ctctgcaata tttggaattt ctgttagttc ccgttcttat
     6181 acgtgcaact atgattgctg aagaaatgac cgctaatgca ataacaaagg ggatagaatc
     6241 tcctgtgaaa agaagttctt actataaaac aaacatgagc ttattagact atatcatcct
     6301 gttttccgtt actatttttt tcttaagtat tttgatttga ggtgaaagaa ttgtttgaat
     6361 ttaacaatgt ttctttttca tataaggatg ttaaggaaca agcgcttgtt gaccttaatt
     6421 tttcaataaa aaaaggcagt cttacattga ttactggttc ttctgggagt gggaaatcca
     6481 cactattaaa gttaatgaat ggacttattc caagacttta tgacggaaaa ttggttgggg
     6541 aaattttatt tgaaaataac tcgctacaat ccttaacaca agaaagtata tcaaaaaata
     6601 ttggctacgt aagtcaaaat cctagaggac aattctttac aacaaataca acgagtgagt
     6661 tagtattttc gatggaaaac tttggattta caaaagagca tatggataaa agaatcaatg
     6721 aattagctga attattagat ctttcaaata tattagataa aaacattttt tcgctatcaa
     6781 gtggtgaacg gcagaaaatt tctattggat gtagtttatc gctaaactct aaattaatta
     6841 tattggatga accgtcttca aatttggact tcaaagcgac taagaaactt gctgacttac
     6901 tttgtgattt aaaaaataaa ggatttacta ttgttatagc tgaacatcgt ttttactatt
     6961 tgaaagaatt aattgatcaa gttctatttg ttaaaaatag aactgtaaaa actacaacta
     7021 ttcagttgtt aaaaactgaa aacaataacg cattaaggaa atttgacatt tttaatagtt
     7081 cgattgaagt tccctatatc gaaaaggtaa gtgcagatgt attaaaagtt gaagaaatta
     7141 cttatgaaaa tattctaaaa gacataaatt ttgatgtcaa agttggagat gttattggtt
     7201 tggtagggaa aaacggtgtg gggaaaacaa ccttactaag aatactcatg aagattattc
     7261 gccctaccaa aggaaagata atcgaaaata aaaaaaattt gtcatcgccg tttttagtaa
     7321 tgcaagaaat ggattaccaa tttttcacag aatcagtgag gagcgagtta gccctgggga
     7381 atgaaatagt tagtaaagat cagcaagaaa aaattttaaa aaaaatggaa ctttataaaa
     7441 tgaaagatca attaccgttt gatttatccg gtggagaaaa acaaagatta ttggtttcta
     7501 tagcttcatt gtcaaaaact aatttatttc tatttgatga acctacaagt gggttggatt
     7561 atatcaatat ggaaaggatt gctgacctta ttaaagactt aaaagaaaag ggtgcagtag
     7621 ttatagccac tcatgatccg gaatttcttt ataaaacatg caatagaatt atttatttaa
     7681 aaaatggtgg aataaaaaaa gatataaaac ttagagtaga agattcaact ttgatagaac
     7741 agatttttaa tgacataacg aaatagaatt gaggagaata tatatgaaaa caaaagatta
     7801 tatttttatt ggtttagcta cagttatttc cctagtaatt tacttttttt caataatggt
     7861 tagtagtatt ggtggggcgt ttggtcacag catatcccca gggatttttg gattattatc
     7921 tggaatcata tttgtttata ttagctataa ctaccctaga aaaggaattt ttactattta
     7981 cacaattgtt ttattgttat tttttacgtt aatgggcggg gcgtacttac cttggtttat
     8041 cagttcgatt agtatggcga tcttagctga tattattcta tctgtatttg gatatgatcg
     8101 agcaattcct caagttgtgt cttgggttct aatgcaacta ggcagtgctg ccggacagtg
     8161 gattcccata tggttcttta cagatcgttt tcgtcaagac tggatagata gaggacaatc
     8221 tgctgcaaca atggatgcaa tgatccacta cgcggtgggt atatggggaa ttatttcagt
     8281 gttagttgta gcaagtttat ctatgattgg tgtattgatt ggaagaaaag tcttaaaaaa
     8341 atacaaaaaa tagaacgtag tttaccttct atctgtttgt acatctctaa tttacgctat
     8401 tacggaaagc attaacagtt gtaaacgaat aagagaagaa attgaatgcg ggggaggaaa
     8461 aaatgaaagt atacaaaaaa ttatttagtt atgtccctaa aatgaaatac tacggatggt
     8521 tagccacttt attttctgat ttttctgtcg tattaacagt ctatggatat tacagtattt
     8581 acaaatttct tcagtctcta attattgtag gagatgaata tttagctaag agttatgcgg
     8641 ttcaaactgt tgcttggtta actttaggag ctttatttta tattttttct ggcttatttt
     8701 ctcatatcct tgggtttcgc ttagagacta atttgagaaa aaagggaatt agcggtttaa
     8761 cagaagcaag ttttcgtttt tttgatttaa attcttcggg atatattcga aagacaattg
     8821 atgacaacgc ggctaaaaca catatggcaa ttgctcactt gattccagat agtgctcaag
     8881 ccatagtaac acctgtttta tcagttgcct taggcttttt cattaacttt aaaattggat
     8941 tggttctaat cgccatgtta gtaattggtg tattcttgtt aaagaaaatg acagagaata
     9001 caaattttat tcaaaaatat caagaatcgt tagatgtgtt aagctctgaa acggtagaat
     9061 atatccgcgg aattcaagtt ataaagatat ttggtgctaa agtaacatca tttaaagcct
     9121 tgaataaagc aattaacgat tatgctaagt ttgcatataa gtattctttg agtgggaaaa
     9181 caccttttgt tctttttcaa tggctatttt ttggcatagt ttgcattttg atagttccta
     9241 taaccttttt tataacttct cttaccactc ccaagatctt agctgttgac ttgttgatgt
     9301 tgtttttttt aagtggaatt atttttgttt ctttcatgag aataatgtat gtaagtatgc
     9361 acttgtttga agcaagatat gcagtcgaaa cactagactc cctttattta gaaatgaagc
     9421 aagaccaact aaaatatggg aacgagacac agattaaaaa ttgtgacatt gcatttgaaa
     9481 atgtcagctt ttcatacaaa gaagagtgtg ttttagaaaa tttcaactta gttttagaac
     9541 aaaaaagaat ttatgcatta gtaggaaaat caggaagcgg aaagagtacg atagctaaac
     9601 taatttcagg tttctacaat gtggataagg gacagattaa aattggtggt aagcctttaa
     9661 atcactattc aaaagacaca attattaaca caattgcttt tgtctttcaa gatacgaaac
     9721 tattcaatca gagtatttac gataacgttt ctatagcaaa tcctcaagcg ccagcaagta
     9781 aagtatttga cgcgctaaga ttagccggtt gcgaatcgat tttagaaaaa tttcccgaaa
     9841 gagagcatac cttaattggt agtaaaggag tttatctgtc aggaggagaa aaacaacgta
     9901 tagctattgc cagagcaatt ttaaaagatg caaagattgt tattatggat gaagccagtg
     9961 catctattga tccagataat gagtatgaac tacaaaaggc ttttcaatat ttgatgaaag
    10021 aaaaaacagt tattatgata gctcatcgat tatccagcat taaatctgta gatgaaattc
    10081 ttgtcttgtc agaaggaaaa atagtagaac gtggaaatca taatgaatta atggctaaac
    10141 atcgagtgta taaacaattg gtcactaatt atgagaatgc aaatgattgg aggcttgaca
    10201 atgaaaaatt tttataaaaa aaaatttgct ttaactgatc agggcgcaga agctttaact
    10261 aaagcttcta tttctagttt ttttgtatac tgcattaata tggtaccagc attcattata
    10321 atgatgctga tagacgaatt agttttagaa aatgcaaagc cccgttggct ttattttgca
    10381 gtatcgtttg ttactttgct tttcatgtat tggttgttag atagggagta tgaaaactta
    10441 tataatagta cttataaaga aagtgcgcat ttaagagtgc aaattgcaga cgatttgtca
    10501 aatttaccat tatcctattt ttcaaaacat aatttatcag atttatctca aactatcatg
    10561 tctgacgttg aaggtattga gcatgcgatg agtcatgcaa tacctaaagc cggcggtatg
    10621 gctctgtttt tcccttttat ttcagtgatg cttttggttg gtaatgtcaa aatgggatta
    10681 gctgttattt tgccaacgtt atttagtttt gtcttaatct tgttatcaaa gaaatcccaa
    10741 acgaaagcca atactaaata ttacgatact ttgagagaaa actcggaaga atttcaagaa
    10801 actattgaat tgcagcaaga gattaatagc tttaatctat ctaaaaaagt tcaagacaga
    10861 cttttcaaaa aaatggaaga gagtgaaagg attcatttaa aggtagaatt aagtactttt
    10921 tcagtcatgg ccttatcctc tattttctca tatgttagtt tagcagtagt cattctagta
    10981 ggtgttcact tactgttaac gggtgaagtg actatactct acgtcgttgg ttacttacta
    11041 gccgcaataa aaataaagga ttccttcgat tctatgaaag aagcagtgct tgaaatattt
    11101 tatttagctc ctaaaataca gcgaattaga gctatgaagg aaaccagtat ccaagaggga
    11161 tcagatagtc ctctgaaatc ttttgacatt gagttgcgag atgtctcttt ctcatatgat
    11221 aacaataccc caattttaga tcatatctct tttacggcta aacaaggaga agtgactgcg
    11281 ttagtaggtg ctagtggttc cgggaaaact agtatcttga aattagtgtc tagactgtat
    11341 gattatgatg aaggctgtat tttaattgat gggtatgata ttaaacgtgt atctccagct
    11401 tctttgttta gcaaaatcgc tattgttttt caagaggtaa ctttgtttaa tacaagtatt
    11461 ttggaaaata tacgtattgg aaatagtcaa gcgtcagatg aggaagtgaa aaaagcagct
    11521 cgtttagcta actgcgaaga ctttattgaa aaactacctg acggttatca tacattggtc
    11581 ggagaaaacg gaagttcact ttctggagga gagcgacaac gcctatccat agcacgggct
    11641 tttttgaaaa atgcgccaat tttaatttta gatgaaatta ctgccagttt agatgcggaa
    11701 aatgaaaaac gtattcagga aagtttaaat cgtttaatac aagataagac agtactaatt
    11761 atatcgcatc gcttgaagtc tattgaaaag gtaaataaaa ttgtagtgat ggatcaagga
    11821 aaactagttg atcaaggtac tcataatgag ttgtatcgaa ggtcagaaat atacaaaaat
    11881 ctcataaaga aaactaaact atctgagaaa ttcgtctatg aaaaagaagc tcaatcacga
    11941 tgatgaaata ggctttaaat taacctacaa tttttgggat atagttataa ttctttttca
    12001 aaaagaataa ttttattaaa cagcatctaa ttttgaatac acgaaagaat cagtatgatt
    12061 gaattagttc acttaatgtc tattcttata atataaggaa taatcaaagt tttaaaaaat
    12121 tggcgataaa ccagttaaac ataaaagatt gaataaaaag ttatagtttc tttcatattg
    12181 ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa
    12241 agtgaattct tatttttcag agcctaaact gcat
//
LOCUS       DAZUMV010000080        12224 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000080 DAZUMV010000000
VERSION     DAZUMV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.080"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..617)
                     /gene="frr"
                     /locus_tag="ACG8FK_001888"
     CDS             complement(60..617)
                     /gene="frr"
                     /locus_tag="ACG8FK_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS5097484.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(620..1342)
                     /gene="pyrH"
                     /locus_tag="ACG8FK_001889"
     CDS             complement(620..1342)
                     /gene="pyrH"
                     /locus_tag="ACG8FK_001889"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS5097485.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1478..2359)
                     /gene="tsf"
                     /locus_tag="ACG8FK_001890"
     CDS             complement(1478..2359)
                     /gene="tsf"
                     /locus_tag="ACG8FK_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS5097486.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2458..3240)
                     /gene="rpsB"
                     /locus_tag="ACG8FK_001891"
     CDS             complement(2458..3240)
                     /gene="rpsB"
                     /locus_tag="ACG8FK_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS5097487.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3599..4078
                     /locus_tag="ACG8FK_001892"
     CDS             3599..4078
                     /locus_tag="ACG8FK_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5097488.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4295..5386
                     /gene="phnW"
                     /locus_tag="ACG8FK_001893"
     CDS             4295..5386
                     /gene="phnW"
                     /locus_tag="ACG8FK_001893"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS5097489.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5379..6164
                     /gene="phnX"
                     /locus_tag="ACG8FK_001894"
     CDS             5379..6164
                     /gene="phnX"
                     /locus_tag="ACG8FK_001894"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS5097490.1"
                     /translation="MIKTVIYDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6510..8201)
                     /gene="argS"
                     /locus_tag="ACG8FK_001895"
     CDS             complement(6510..8201)
                     /gene="argS"
                     /locus_tag="ACG8FK_001895"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS5097491.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8623..9570)
                     /gene="arcC"
                     /locus_tag="ACG8FK_001896"
     CDS             complement(8623..9570)
                     /gene="arcC"
                     /locus_tag="ACG8FK_001896"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS5097492.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9685..10704)
                     /gene="argF"
                     /locus_tag="ACG8FK_001897"
     CDS             complement(9685..10704)
                     /gene="argF"
                     /locus_tag="ACG8FK_001897"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS5097493.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10795..12024)
                     /gene="arcA"
                     /locus_tag="ACG8FK_001898"
     CDS             complement(10795..12024)
                     /gene="arcA"
                     /locus_tag="ACG8FK_001898"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS5097494.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3356 a         2541 c         2194 g         4133 t
ORIGIN      
        1 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
       61 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttaatgctg
      121 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      181 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      241 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      301 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      361 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      421 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      481 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      541 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      601 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      661 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      721 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      781 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      841 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgctcgta
      901 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
      961 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1021 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1081 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1141 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccacccaca acgattgcca
     1201 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1261 gtttgattcc aaatcctgca tccccagcta aagcttctcc actcaatttt aagactacac
     1321 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1381 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1441 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1501 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1561 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1621 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1681 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1741 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1801 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1861 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     1921 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     1981 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2041 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2101 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2161 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2221 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2281 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2341 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2401 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2461 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2521 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2581 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2641 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2701 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2761 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2821 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     2881 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     2941 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3001 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3061 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3121 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3181 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3241 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3301 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3361 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3421 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3481 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3541 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3601 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3661 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3721 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3781 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3841 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     3901 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     3961 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4021 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4081 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattaa
     4141 ataaaccttt acactcttta caaaaataca tcatttttta ttcaattttt tcaacacctt
     4201 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4261 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa ctcctggacc
     4321 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4381 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4441 ggaaggaata tacacaacgg tcttaatgca aggtagcggc tcctttgggg tagaatcggt
     4501 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatgg
     4561 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4621 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccctctat
     4681 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4741 gattgctgct gtcacacaag aaaaagatct tttgtttata gtggacgcga tgtcaagctt
     4801 tggtgggata ccgattcgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4861 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactaga
     4921 aaagcgaaaa aatgtgagtc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     4981 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5041 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5101 cgccaataat catgtactac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5161 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5221 taatcatttt tatcagtatt taaagcaaaa cggatttgtg atctatcccg ggaaaatcag
     5281 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5341 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttatg
     5401 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5461 cttttctaga aaaaggaatc caactgactg acaaagaaat ccgcgaaccg atgggaaaat
     5521 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5581 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5641 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5701 tcgaacggtt gagaaacgct gggatcaata ttggttccac gactggattt actgctccta
     5761 tgatgacaat cgtagcagaa accgcaaaaa aagaagggta ttcaccagac ttcatggcaa
     5821 cttctgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     5881 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     5941 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6001 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaaagat cgtttaaaac
     6061 agtctgttgc tcttagattc caacaagctg gcgctgatat ttgtgtaaac aatttaaaag
     6121 agttaacaga atacattttg tacgctgaca agaatccaga atgactttta tacaaaatca
     6181 gaggtcgtga aaaagctgta ctgcatcaag taataagaac agccaaacaa aaatagctcc
     6241 tcatattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa caccgtttat
     6301 tcgaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt tacaaacaaa
     6361 cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact ctcggtattt
     6421 gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta atactcggat
     6481 cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg aagtcctagc
     6541 aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa gcgtgcttct
     6601 ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa tgcttgtgct
     6661 aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc agacaagacc
     6721 gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc attcaatgca
     6781 tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac tgcacgagca
     6841 tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc ttccaggtta
     6901 aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc cccaacccca
     6961 acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct gatttgttct
     7021 ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt tcctttacga
     7081 gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggcg catatcgtca
     7141 gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg atagctttgt
     7201 tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg gtacaatgca
     7261 gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat caacgctgga
     7321 ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt
     7381 ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc
     7441 aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt catcgattca
     7501 tcacggaacc attgccataa ttgaatagcc tcttcatcgc cttcttccag acgtttgaac
     7561 caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg gaattggaca
     7621 tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt accccatttt
     7681 ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg gttgatgcgg
     7741 attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat aactgtcgaa
     7801 cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc gattggtact
     7861 gttccttgat tcccgatatt gctatcccca taatgttctt tttctttcac tactgtttgc
     7921 aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg tccgacaact
     7981 tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc gatttgctgt
     8041 ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc tccgtgttca
     8101 gctgattttg gattttctag taattgttcg acttgttcca gtgttaaatc atctttgaca
     8161 gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc tcctaaagtg
     8221 ctcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta tgccatcact
     8281 agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca gctccatctg
     8341 atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc gttgtatctt
     8401 cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa gtactctctt
     8461 gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa aaaacgaaag
     8521 gctagtcgtt tctgataaaa aaacaatcca gatggcctaa aggagaagaa ataccacctg
     8581 gatagctata aatcatttcg taaatacgaa agacttaaca aattaatcag ctacgataat
     8641 cgttccttct tcagaagcta aaagattctc gatattttct aaagaagtga tgattgcctt
     8701 agcgtttggt tttgcttcaa caaatgcaat agctgcctct actttcggaa gcatgcttcc
     8761 tggagcaaat tgcttttcat caatgtattg tttcatttct gaaacagtaa cagtttctaa
     8821 tttcttttga tctggttttt ggtaattgac atacacattg tctactcctg tcaatacgat
     8881 cagcaaatcc gcatcgatga tttcggctaa tttttctgaa gcaaaatctt tatcgattac
     8941 ggcttctcgt ccttctaatc cagtagctgt ttctacaaca ggaatcccac cgccaccaac
     9001 agagactgtg ataactcctt gatcgaccag cgtttcgatg acacgtgctt ctttaatcga
     9061 gatgggttta ggggaagcga ctacttttct ccatccacgt ccagcatctt ctttgaatgt
     9121 cacagtggag tcagcattca tttgttcttt ggcttcttct tccgtgtaga atggaccgac
     9181 tggtttactt ggatttttga atgaaggatc attttcatcc acaataactt gagtgactaa
     9241 agaaaccaca tctttatcga tccctttttc cttcagtact tcgcccatgg cattttgtaa
     9301 ccagtaaccg atagatcctt ctgtcattgc aacacatgta tctaaaggca ttgccggtgt
     9361 tttctcagaa tcagcagctt gctgctgaat caataggtta ccgacttgcg gaccatttcc
     9421 gtgggagatg atcaattcat ctccttgttc gataaatttc actaaatact ttgctgtttc
     9481 cattaacgct tcttgttggg cttttgcact tgcatccgta gacaagattg cattaccccc
     9541 taaagcgaca actacttttc gtttggccat ttcattcgtc ctttcttcag ttctaccgaa
     9601 ttttctagat aacaaaacga gccagcgttt tgtgattttc cctcaatgct gactcgtttt
     9661 ttcgtgagat gaatgtggtt atgcttaaac gcgagggata aacaagttac ctaatgttgc
     9721 tgccatgatc gctttgattg agtgcatacg attttctgct tggtcaaatt gacgagcata
     9781 tttgctgcgg aagacttcat ctgttacttc catttcagtg atgccaaagc gttctttcat
     9841 ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc aagcagtgca agaagatcaa
     9901 acgatctgtg ttgtgtgttt tttctaccat ttccatattg atttggtaag gtttcaacaa
     9961 tttgatacgt tcttcgaatt tatcttcttc acccattgat acccatacat cagaataaag
    10021 gacgtctgct ccgtctacac ctttatcaac atcgtcggta atcatcaatt gtgcaccgga
    10081 tttttcagca aagccttctg ccattttgac gatttcttct tcaggttgta attcttttgg
    10141 tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg actaataaag agttagccat
    10201 attgtttcga ccatctccgc agtaagcaac agtgatacct tctactgttc caaagttttc
    10261 ttggatagtt aaaaagtcag cgatcatttg tgttggatgc cattcatcag tcaagccatt
    10321 ccaaactgga acaccagaga attctgctag ttcctctacc attttttgac tgaatccacg
    10381 gaattcgatt ccatcaaaca tacgacctag tactttagct gtatcttctg tggattcttt
    10441 tttacctaat tggatatcat ttgcacctag atattcagga tgtgcaccta gatcgatcgc
    10501 tgcagtagta aatgcagaac gagtacgtgt agatgttttt tcaaataaaa gcgcaatatt
    10561 ttttccttct agatagtgat gtgggatacc acgttttttc aagtctttca agtgttctga
    10621 aaaatcgatc aaatattgta attcttcttt tgtaaagtct ttttctgcta ataagcttct
    10681 tccttggaat actgattctt tcatattaaa cattctcctt tagattgttt ttatttaaga
    10741 ttaaagagga aatcccgacg gcagctgaca gtaagaccgc cgtcaggaaa caaattactt
    10801 caagtcttca cggacaagtg gttggctcat gcagcgtgga ccaccacggc ctcgtgacaa
    10861 ttcacttgaa ttgatttcta acactttgat gccatagcta cgaagcaatt cgttcgatac
    10921 ataatttcgg ttataagtga ctacaacacc tggagcgatt gccaatgtgt ttgatccatc
    10981 attccattgt tctcgaggtg caacgatctc atctccatta ccagttggga tcagtacaag
    11041 atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag ttatctgaat gagtaatctt
    11101 gatatcatca ccatctggtt cgattgtaaa gacatccatc ttaccgtctt tactttgaat
    11161 tgctggatgg atagtgaatt tatcgtgatc taccattgta aatacggtat ccaaatgcat
    11221 cattgcacgg ttgtttggaa ttttgattgc taacactttt tcaaatcctg aattttttgc
    11281 gaataggtgg cgtgctaatt tttctaaagc tttcgcatta gttcgttgag aaatcccaac
    11341 agctactact ttatcgctta agatcaattc gtctccgcct tcgatatgat ctggattttc
    11401 acgatccaac catacttcga ttcctttatt tgcaaatctt ggatgatgtt tcaaaatata
    11461 ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc atcttattga ctgtcatccc
    11521 atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag tttggcattg ggtccatgta
    11581 aaatggatag tcatcgtctg ctgacagatc gtatagactg ctagaacgta cttcaatatc
    11641 ttttgtacga accccagcca tgattttatc taccatttct tgcgtttcca ttgatagaag
    11701 gaactcatga agaccctctc ttacagcatt cgatgcaata tgagattcat ccaacatttt
    11761 attcaagaat tgttctttga cattgcctgc gtcaatcgct tctgccgcta atttttccaa
    11821 gtaaagagtc tcgacgcctt cattttgcaa tgtctttgca aagttgtcat gttcttcttg
    11881 agcaatcgga agatatggaa tatcatcgaa caacagacga tccataatat ctggtgtcaa
    11941 attttccact tcttgtccag gccttttcag taaaactgtc tttaatttcc ctatttcaga
    12001 gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt ctttttgatt acatgtatag
    12061 gatatcctcc ttttgtaatc actttcataa ttttgtctca ttcaaaatga gacattcctc
    12121 acattaatat tcgataatta aataattaaa cgtttttcta tttttaagcg taatttattc
    12181 attttttatt ctgtatacac tttcaaaaaa atatttattc attg
//
LOCUS       DAZUMV010000081        12202 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000081 DAZUMV010000000
VERSION     DAZUMV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.081"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1148
                     /locus_tag="ACG8FK_001899"
     CDS             105..1148
                     /locus_tag="ACG8FK_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS5097495.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1321..1545
                     /locus_tag="ACG8FK_001900"
     CDS             1321..1545
                     /locus_tag="ACG8FK_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097496.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1548..1805
                     /locus_tag="ACG8FK_001901"
     CDS             1548..1805
                     /locus_tag="ACG8FK_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097497.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            1907..2260
                     /locus_tag="ACG8FK_001902"
     CDS             1907..2260
                     /locus_tag="ACG8FK_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097498.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            2313..3107
                     /locus_tag="ACG8FK_001903"
     CDS             2313..3107
                     /locus_tag="ACG8FK_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097499.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3136..4254
                     /locus_tag="ACG8FK_001904"
     CDS             3136..4254
                     /locus_tag="ACG8FK_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097500.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4287..4607
                     /locus_tag="ACG8FK_001905"
     CDS             4287..4607
                     /locus_tag="ACG8FK_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097501.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            4862..5551
                     /locus_tag="ACG8FK_001906"
     CDS             4862..5551
                     /locus_tag="ACG8FK_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFS5097502.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            5588..5755
                     /locus_tag="ACG8FK_001907"
     CDS             5588..5755
                     /locus_tag="ACG8FK_001907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097503.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            5775..7403
                     /locus_tag="ACG8FK_001908"
     CDS             5775..7403
                     /locus_tag="ACG8FK_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097504.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            7454..7825
                     /locus_tag="ACG8FK_001909"
     CDS             7454..7825
                     /locus_tag="ACG8FK_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097505.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            7910..8110
                     /locus_tag="ACG8FK_001910"
     CDS             7910..8110
                     /locus_tag="ACG8FK_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097506.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8244..9470
                     /locus_tag="ACG8FK_001911"
     CDS             8244..9470
                     /locus_tag="ACG8FK_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097507.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10320..11645
                     /locus_tag="ACG8FK_001912"
     CDS             10320..11645
                     /locus_tag="ACG8FK_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5097508.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            11638..11985
                     /locus_tag="ACG8FK_001913"
     CDS             11638..11985
                     /locus_tag="ACG8FK_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097509.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            11982..12164
                     /locus_tag="ACG8FK_001914"
     CDS             11982..12164
                     /locus_tag="ACG8FK_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097510.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
BASE COUNT         4742 a         1506 c         2446 g         3508 t
ORIGIN      
        1 acaacaacga ttatgatctt aaaacaatcg tttatgtaat tggcaatctt acgaataatt
       61 ttcatgcaat aatgacaata aaaaatgaaa aggggtaaaa tcaaatgaaa aaatttttag
      121 taggaattct attcacaatg gggctagtat tattatcacc ttcagtttca gcagaaagct
      181 ctatttccat gtatcgtatg tataatccca atagcggaga gcatctatac actagaagcg
      241 ttggagaaag agacaatctg aaacgttatg gatggcatta cgaaggtgtc gcatggcaag
      301 ctccaacaag tggtactcct gtttatcgtt tgtataatcg gtataacggt gaacattttt
      361 acacagttga tgccggagaa agagataaga tttcacacac aggatggacg tacgaaggtg
      421 ttgcgttcta ttcctacaac ggagcaaata gagttcctgt aacacgttta tataacaaat
      481 acgtgaattg gcatcactac acgttagatc aaaatgaaaa gaacgtaatt agtaaacaag
      541 gttggaagat tgaaggtaca ggctggtatg caatgccaaa cagcgtacca aatcctacac
      601 ctgctccaag cactgaaaaa gtgttgaacg ttcctgttgt atggcaagga aatacaatgt
      661 tatgcgaagg agcttcttta ttagaaggtt tacactacaa aggtgtagcc acaaatcaaa
      721 gcttaaccag ttttgtaaac tatatgccaa aagctaatga caataatcct tatcatggtt
      781 actcaggaga gtggcgtcat aacgttaatg gaacgtatca aggcatgatg gctgacccag
      841 tagttaaatg ggcaaaaaac gttggcggta atgccgtcaa tatcactggt tcaggaacga
      901 atggaatcaa gaatgaaatt cgcaatggta atccggttgt aacttggatt acttataact
      961 atgctacgcc agagttcaaa caaatgcctt ggggtcgtgc agtatggaat ggacatgtag
     1021 ttctcgtgga tggcttcaga aatggtgcat accatgttgt tgaccctgta tttggcgcaa
     1081 aatggatcaa tgcaagcacg tatgagagag catttaatgt gactggaatg gcggttgctg
     1141 tacgttaatt gatagaatta tcaactaaat gaaataagag cttaattact gaaatggtaa
     1201 ataggctctt atttttttag aggaaatttt tgtaatacga atctataaca aaaaatattg
     1261 aatgtagtac acaaaagtgg tatattaaaa tcgtagagat tatgcgaaga ggtgattttg
     1321 atgaaccaaa gaagaagaaa aaggttagaa gaaataagcg agcgactttc cgagctaaag
     1381 gatgagttag aagtaattca agaagaagag tacaaagaaa atatacctaa aaatctacag
     1441 ggatcagaaa gatatgaaaa agcagaagaa tccgttgatc aacttgaaga agctgttgat
     1501 ggtttatccg acatattaga attgattgaa gatatagcta gctagagatg attaatgaag
     1561 atttcaggag tgagaataaa atgattgata tttcactgat agagaagtta cttttctcaa
     1621 ataagtacac cgcaaaagag ctggctacaa gatggcaaat atccgaatca gctattaaag
     1681 cctatcgtca aaataaggga agcgtaggat ggcgtgattg gaaagggatc agcattggaa
     1741 aagcagaaga cataatgaaa cgtgaaggac ttcgatccta taccacttct gaaaaagaca
     1801 aataacttaa tgtttgaaaa gataaaatat ctgtcttaaa gctatgtcaa attcaaatgg
     1861 tgaatgtttg ctaaatataa tgcaaaaaaa ggaagggaaa tattaaatga taagcgaaaa
     1921 tttgtcttat gagaataata aacatctagt taaagaaaat aagaaagtag aagattataa
     1981 gaacatgtat atagaaatga aaaaatcacc actgtgtatt caatataaaa atgataattg
     2041 gaagcaagta gtcgaatttg taaaacctca tagtatacga agagtacgaa gggggaagaa
     2101 aaaaagtgaa cttgtaaata gcggaagagc ggtaattaga gttataacca aagattatcc
     2161 agttgaatat ggagattata ttttaaggtt aagttatcat gcttttgttg tgttgaaaga
     2221 agagcatttc aacgagtttt tcaatttaaa aagtaagtaa tcatattttt ttgtagacgt
     2281 gtataaagtt aactaaattg cgaggtgatg acatggtagc aaaaaagaat actctaaaag
     2341 aagcttctga aaaatatcaa gatatgaaaa gacgaggatt aataccgaaa gacaaaaaag
     2401 gtatatggag agaatttgtt aaggaagtca gtagtgatcg aagtggcggt gactttgttg
     2461 gacgatatac agcaaaaggc tatagcaaac gattaaggat cactgttgaa tatggaacga
     2521 atgaatcagc tacagagctg aagcaaaggt acttagatac acgaagggaa gcttcaaaaa
     2581 aatataatga tttcaagagc agaggtctga ttcctaaaaa gaaaaaggga atttggcgtg
     2641 agtttttcaa ggaagaagat agcaatcgga ataagggaga cttcattgga cgatacacag
     2701 caaaaggcta cactaagaat ttagggatca aggttgaata cggaactggt gaaagttcat
     2761 gggaattgag aaatcgctat ttaaatacat tgttagagaa atataaagca gaattattgg
     2821 agttgttcga tgttaatgat tttatcgaga tggctgaaaa gtatggtcat gaagaaggtt
     2881 ttatgatcaa tgacattatg gttattgata attttacgga tttattagca cttgcgaaaa
     2941 aagcaaggga tttaagagaa acgcagatgt atctattcag aaataaaaac ggaagaaacc
     3001 ggagaactga atcgagtgag attgaagatt actacgatgc ctttacacac gatctgggag
     3061 cctttgtaga cgagataagt ggtgtaatga acaaatatag ggtctagaat aaaaaagccg
     3121 gagatgagca gataaatggg aaacaatagc atatacacac ctgaaatgca gaataaaatt
     3181 aaaagacttg tcggattagg gtggatgaca agaggaaaat tagattcttt tagtacgatc
     3241 aaatatcttt tgatggacat ttaccgtcag aacaagcaaa caggaataca ttttttgggc
     3301 tatgataagc cggaagatgc acctttcttt gttccaagtt cagcctatag agccagtacc
     3361 tccggtagca tgtataactt ggatttgttt gaggaaggag caaagatgat cgaatatgat
     3421 ttcaacgaag cctattcgag gatcatgaga aattatcctt tgccgtcaaa cacttatttg
     3481 aaagcaaaat ataatacaga tcaagctatt ttgagagcga taaatagacc aaaagtaaga
     3541 ccgtatgaaa atttaagaac gtatatgttc tttttggtta attttgaagg aacagccaaa
     3601 gaaggaacat atgctaatcc aaaatcacag tcgatgtttg cggattatgg cgaaaatccc
     3661 aaaacagcta atgagcctat ttggttatcg gagattgagc taacacttat ccttgattat
     3721 tatgatatta aagagatgga agtcctagat agccacacat ttttgacgaa aaaaggatta
     3781 ctcaaagaat attttgaaag aatcgagcca ttaaaaactg atcctgatac gttgaagctt
     3841 tataaaaatc ttagaacgaa agtatttggt gctatcggac aaaaatcatt aagagaagga
     3901 gaaacaagca ccttcgataa atacagtatg tataatcgag cgtattcctc catggtagca
     3961 ggcgtattta gagatcgtat ggttagatat gaacagaaat acgttcatag cgcatatggg
     4021 ctattaaaaa ttcggactga tggattgtat ttcagatccg aagtaccaga gtttgaagag
     4081 ctgtataaac ttggagtagt taagaaaaaa gttcatacaa tatccaaaga agaagtcgaa
     4141 aaagctttag aatttaaaaa caaaggattt gaaaataagg gctcctttgg gaaatctaaa
     4201 gatgaagtta agggtgttgt gatagacgat gatttcgagt ttgattttga ctaaatactc
     4261 tttggaaaag atggtaggag gtaaatatga agaagaaaat tgtggaagga aatgtaggtg
     4321 ttattattct aaaagattca actgactatg ctaaatcaat ggcactaaaa gacgaaaaag
     4381 ttgcacgcat ggataaagat ttagttgaat ttctgatgga tgatacagaa agattaaaag
     4441 gggatcagtt tttgagttta atgaaagaca aaggcttaaa cacatatggt ttatttgatt
     4501 ataagaaatt agctatagag tgggtaaaag aaggagaaat attttttatt gaagaatggg
     4561 aaggtactga gaacttagtt cttcatcagt atcttttgaa agcttaatct aagtaaaaca
     4621 gttctgacta gttatgcttg tcagaatttc aaaggaagtt ttccataacc atcatcattc
     4681 aaaaataaag gatttgaaaa taagggttcc tttgggaaat ctaaagatga agttaagggt
     4741 gttgtgatag acgatgattt caagtttgat tttgactaga tttttttgaa aggtttaaga
     4801 gtaaacaagg taagaactaa gatcatgaag attgagagta aagaagatag aggacgaatg
     4861 tatgagaaca cactatattt atttagaaga taaaattgta gatgaaattc cggcaaaaag
     4921 tcgagaacaa gctagaaaat tatttattga aaaatacaaa gagatcaatg gagatagtga
     4981 agttccaaaa gtggtcgtaa cagttagaaa aaggcaaaca aaaaagagaa gtacgtttca
     5041 tgtcagccct gatatatata atcttataga gcgacataga agtgaagata atatttctat
     5101 ctctcaatat attgctacag ttattgatga attgatttca ggaaagtatg actatgcttt
     5161 tgattttgag ggtgaaaagg attacaaaag tgttgctgtc tttcaatctg attttgagaa
     5221 gctaaaaaac tatgctggca ggagaaatgt taattttgca agactactag aagagttcta
     5281 ctccgaagag ttgggagtag gaaatgaata caagagagaa acttatgcga aattgtctca
     5341 taagttatcc caagcgctta aagaagtgac agaagttaaa gcggaactag atgctaaact
     5401 tgaagaagtg aaaaaatatc aaacagagag gaagaacaac atgaagaatc aagtggaatt
     5461 tgacgaacag aaagaatttg aaaaagcaag aaaacgctat aacaaaatta tgaatgaact
     5521 tagctatgaa cacttaacag ttcatgaata attgagtgag ggtacagaaa aatatacgat
     5581 cgcagatatg gttaatgaag tggaaactct tttagcttct ttctttgaaa atggtcacgt
     5641 aaacgatgaa tggcgaaaag aagaacctaa aagggttaac gcttatattg ctcgattaaa
     5701 aaggtttatt gataaatata aggagaatag tgacaaattt tctaaagata aataaggaga
     5761 gagtgtgatt acatttgcca gaaatcgaag gaatattttc gccaaaaata ggtaaaaaac
     5821 agttagattt cagcagtttt ccgagcgtaa atagagatta taatctaggt gatgaggtac
     5881 agatactaaa tattgatttc aaagaactag acttcgctgt tgctgtttcc aacaaaccca
     5941 aggcattgga acgagtgaaa aaaatatacc aagagaatga tcaaaatatc aaaaatatga
     6001 tcaaaaagta tagacgagat aattcaaaag ttgaagacgt tttattcaac ggtacggcaa
     6061 aacatgattt gaatgtttat atggcattag gagtggcttt gtactgtaat tcaattaatc
     6121 agacagatat tttggttaat atcgtgagga atagctatca aaaaatatta aaaataagta
     6181 gcgaaacaaa cccttattat gcaatgaaat atcttgggga taatttatta aatgataaaa
     6241 aattgatgca caacactgaa gagctgttat ctaatttggc tgcgattaac tttctattca
     6301 aaacaggttc tgaagattat tttatagata acgtactaaa aggtgcttac ggaacagcat
     6361 tttatccgct ttacaatatg aaacagcata aagccgaaca aaaggataac aaacttgaaa
     6421 ttaaaaagat gaaagaacta ttaggtatag gatttgtaaa acccaattca aaagtcacta
     6481 ttgcttcgtt agcaactact aacaatgatg ggaaaatagt aaatgggttt gacgattcag
     6541 atatagatat attgtcaaaa tcatattttt ctttgtatgg agattttgca gatttgatgg
     6601 gtattgatag ttacttagtc aataattttg agatcaaggg tagcgagctg aatgatattg
     6661 tgtatttcgt tttaatctct ctatatgcaa ttgatcagaa taccgagtac cgacaaattg
     6721 actatttgct agttttttat agtttgttat atcagttcgt tttaactcgg ttgtttaaaa
     6781 atgctaagga agaattgatc ctgaattctg attatgaaat agcaaaacaa attgcaagta
     6841 ttagaaaaga aaacaaatct gaagtaatca aattaagaaa agaattaagt attgcgagta
     6901 aagaaaacgg tcggttagaa tctttagtca aatcggcaaa aaaagaagag gaacttttga
     6961 agcaagagat taaagaatta aaaggaatca gaaaagaact tgaacagctg aggtttgaaa
     7021 ataaagtatt aaagcaattg caggagcaag aacatagaca atcattaaat ggtaaaactg
     7081 aagagccttc actgtctgaa atgatagctt atctaaaaca taaaaacatt cttttagtgg
     7141 gaggacatgt aaattttcaa aataaattaa ctgaactatt accaaatatt aaaatgtttg
     7201 atgtaagcga aaataatgga aagatactta aaaatttgaa tacgtgtgat gcgatttttc
     7261 tatatacaga ttacttgaat catggtctat attatcgtgt tatgagtgaa atttctaata
     7321 aagataagat attttatttg gataatcatg tgaatatgaa cttaacaatt aaacgtattt
     7381 acgatctttt atcacttata taaagagaga agtgataaat aggacacaaa atttggaaag
     7441 taggtaagtc actatgagag aactaacttt atcttctaag gaattagaaa agaaaactgc
     7501 tttattaaac gaaatattta gtgctgattg tataatccaa agtttaaaag aaagttcatt
     7561 ttcaaaacta acagatcagg aaaaaatttc ttatatgcta agtttagata atctaggaac
     7621 taaatatgat gaatcagcaa gtagcgataa gcttttgatt gaattagtat caagtcttag
     7681 tagttttgga aaaatgaaaa gatttaatgt gatattagaa atttttgctt ggttaatccc
     7741 ttcatttatt tggttttttt atattttcat agttgaatct gagttaatag ccaaggtctc
     7801 tcttggactg ttaattccga tataaatagg ttcagcaatt tactttaata acttgtatac
     7861 acgtattcaa ataaaaaagc agttattaca tgaaggatta agtggtaata tgttaaaaag
     7921 aattgcatgg gataaagata ataaaacaga tattgagctt aaagaatata aatacaatta
     7981 tttctcgaga tgtatagata gaaagttact gtttaaaaaa gaaggattag aatttagttt
     8041 tgatgaagat gaatttgaaa aattagtgat ggactggcgt aagaatttac tggatacggt
     8101 acaattctaa aagcaattga ggtcgaaatt ccgatacttg aatcatgatc tatattatcg
     8161 tgttatgagt gaaatttcta ataaagataa gatatttcat ttggacagtc atgtgaatat
     8221 gagcttaaca attaaacgta tttatgatct tttatatgtt tataaacatt aatgaggtgc
     8281 taaaattgac aacaataact atgacaaaaa aaatagaagt atatgttgat ggatcacaag
     8341 aagaaaggga ttcctttttt gatttttttt ggaaaaatag tagagaatat gtaaaagcgt
     8401 tgaatctggt atataaacag ctaatgtttg aagagttgat aaaagatgca attaaagaaa
     8461 tggatcaagg ttatgctgag agagaagaaa gtattcaaaa tcggattgaa aagttagcat
     8521 atactgatcc aatagatcaa aaaaaacttg ataaagcaaa agctgaatta tctaaattga
     8581 gaaatgaaaa aaataaagaa gcgagagaaa tattaacgca agcgataggg ttgaagagtc
     8641 aaacgagaac acgagatgta gcaaaaaaat tagactttga ctttggagat tttgttgatt
     8701 ccgcaaatat gaaagctagt aaagattttg gcaatgattt tatcggaatt gtgaccggac
     8761 aaagaacgcc tagaatatat aaaaatagaa acaaatatgg attactacct tttagaggtc
     8821 gagatattaa aatccataaa gatgataagt tttatattta tttacctaaa ggctttaggt
     8881 ttaaagtgaa tttaggtagc aagccaagaa aagcaagaga tgttgcttca gttttacaac
     8941 acattatgga tggcgattac aaattaaatc aaagtaatct tatttttgat ggaagaaaat
     9001 tattttttaa tctaacaatt tcttttgaaa aaagctataa tgaaatggaa ttcaagaaag
     9061 gtaatgaatt gatcgtaaac tttgcagatt tgtgtagcgg tgaatgttta attaatgata
     9121 aatctgtaat gtcttttggt gatagccaat atataagaaa cttcaaaagt aaaattttag
     9181 caatgaaatc aaaagaacaa agccgaggag tcttttctaa gggcggtcat ggtcgaaaaa
     9241 gaaaattaga tcaagatcga tgggaagcaa taaagaaaag agaaagtaat tttgttaaaa
     9301 cttataataa tcaatgttct gcaaaaatcg ttaaaactgc tattcgcaat aaagctcaaa
     9361 ttattcgtat cattgaacca gataaggctt atggcgaatg ggcttactat caattgaaag
     9421 aacaactagt aaacaaagga aaaagatatg ggatagaagt cagaagttag taaattaaac
     9481 aatcgttgat ttggcaacga aatagtgagg gaaaatccac tcactgaagg gtaaactccg
     9541 tcaagttgtg tgaccagacg gaaggacgaa tgttgttgag gttttagaac catataaaac
     9601 agcatgtttc tcaaattttt aggataaggg aaattgctag cagtttgttt tagaaccata
     9661 taaaacagca tgtttctcaa atgagtactt taaattaatt gatggcgttc ctgctttaga
     9721 accatataaa acaacatgga tttaaaacaa gaagtaagtc agttctcctt ttactttagt
     9781 aaggaaaaat aaagtattct caataaaact taaaataagc aaacctttgt tttaatagct
     9841 aatcgaagta aaacccaaag ccatatgaac ccctgaatca gcgaaagttt atgatgattt
     9901 ggattactaa tagtttagga attagatggt aaatctaaat taaacaatcg ttgatttggc
     9961 aacgaaatag tgagggaaaa tccactcact gaagggtaaa ctccgtcaag ttatgtgacc
    10021 agacggaagg acgaatgttg ttgaggtttt agaaccatat aaaacagcat agttctcaaa
    10081 ttgtttaaaa tgattgtcaa gttaaaaaaa tctgggatca tataaaatag tatagttctc
    10141 aaatatgatt agaaacccta ttaaagagca atattaagcc aacttactta aatcgtttaa
    10201 ttaagaacaa taatctcttt tagtcagact tttagtctga cttttttgta tttccaaaat
    10261 ttattcttta caaaaacgaa catacgtttg tataattgct tgtgaatggg ggaataaaaa
    10321 tgtttgatta cagtagagaa ccggtgaatg actatttttt aatcgacatg aagtctttct
    10381 atgctagcgt ggaatgtatt gaacgaaatt tagacccaat gactacaccg ttaattgtca
    10441 tgagtagatc ggacaacact ggttcaggtc tgactctagc ttcttcacct actgcaaaga
    10501 aaaaatatca tattactaat gttagtagag ctactgattt accttatcca ttaccaaaag
    10561 aacttcatat tgttccacca cgaatgaact tatatataaa aaaaaatatg gaagttaatc
    10621 agatttttag aaagtatgtc gcagatgaag atttattgat ttattcgatt gatgaatcaa
    10681 ttttaaaagt gacaaaatcc ttaaatttat ttactagttc aggaacaaga agcgagagaa
    10741 gaagagaatt agctaaaata atcaaagatg agattcagaa agaaacaggt ctaatagcaa
    10801 cagtcggaat aggagataac ccattattag ctaaacttgc actggacaat gaagcgaaga
    10861 gtagtaaaga atttatagct gagtggactt atgagactgt acctgaaaaa atatggaaaa
    10921 taaacgagat gactgacttt tggggcattg gatcacgcat gaaaaaaaga ttgaatcata
    10981 tgggaattat gagtatagaa gatttagcaa attggaatcc ctacacaatt aaaagtcagt
    11041 taggtgtaat cggactccag ttatatttcc ataccaatgg aattgatcgt acggatattg
    11101 ctattccacc agaaccaacc aaagaaaagt cttatgggaa ttcacaagtt ctaccaaaag
    11161 attatacgag gagagaagaa gtagaactag tagtcaagga gatggcagag caagtagcca
    11221 ttcgaattag acaacacaat tgcaaaactg gttgtgtacg cttagcaatt ggtacgtcaa
    11281 ttttggaaga aaaatcaggt ttctcgcacc agatgaaaat accaattaca gataatacga
    11341 aagaattaca aaattattgt ctttttttat tcaataagtt ttatgagggg caggaagtac
    11401 gacatatagg agtaacttat agcagattag tatacacaga ttccctacaa ttagatttat
    11461 ttagtgatcc aagaaaaaag attgctgaag aaaatttgga taggattatt gataagatta
    11521 gacaaaaata tggatttaca gctattatac atgcttcatc tatgttagaa ggtgcaagaa
    11581 gtattacgag aagcacttta ataggaggac acgcaggagg attaggaggt ttgcataatg
    11641 attgaatata acgatagagg acgtttaaaa tggtcgggat tttatttgtc tgatcatact
    11701 gaaagaatca atgcagacaa tgtacaacgt gctaatcata atttggcaaa agaacaaatg
    11761 acgactgaag agattgcgaa aatactgaga actgctgtca taaaaaatag acccgtctgc
    11821 attcaaaaag aggaacgaaa cgctgaaggt tactatccag atgatgtagt aggaaaaatt
    11881 attggatatg acgagcttgg tgtatttgtg gaagaagaaa aagttcacta tgatgaaatt
    11941 aggaatgttt ctttctcgga attattaaaa tggagtcaga tatgaacatt attaaaaaat
    12001 acgaattcgg ttatattacg tatgaacagc taatagagga attatgggga tatggtcaac
    12061 aattaattaa tgaagtgggg attgattgct tttgtttcta tattgaatcc ggagcaggat
    12121 accataagaa cagatactat atatttccat atccaccaaa atagtctctt agatattttt
    12181 aaattttctc caaatacttt ca
//
LOCUS       DAZUMV010000082        12174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000082 DAZUMV010000000
VERSION     DAZUMV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.082"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..300)
                     /locus_tag="ACG8FK_001915"
     CDS             complement(136..300)
                     /locus_tag="ACG8FK_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097511.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            595..1923
                     /locus_tag="ACG8FK_001916"
     CDS             595..1923
                     /locus_tag="ACG8FK_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS5097512.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2125..2958
                     /locus_tag="ACG8FK_001917"
     CDS             2125..2958
                     /locus_tag="ACG8FK_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS5097513.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2974..3711
                     /locus_tag="ACG8FK_001918"
     CDS             2974..3711
                     /locus_tag="ACG8FK_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS5097514.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3866..4834)
                     /locus_tag="ACG8FK_001919"
     CDS             complement(3866..4834)
                     /locus_tag="ACG8FK_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS5097515.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5066..5899
                     /locus_tag="ACG8FK_001920"
     CDS             5066..5899
                     /locus_tag="ACG8FK_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS5097516.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5933..6772
                     /locus_tag="ACG8FK_001921"
     CDS             5933..6772
                     /locus_tag="ACG8FK_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5097517.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6798..7358
                     /locus_tag="ACG8FK_001922"
     CDS             6798..7358
                     /locus_tag="ACG8FK_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5097518.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7557..9278
                     /locus_tag="ACG8FK_001923"
     CDS             7557..9278
                     /locus_tag="ACG8FK_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS5097519.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9443..10585
                     /locus_tag="ACG8FK_001924"
     CDS             9443..10585
                     /locus_tag="ACG8FK_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5097520.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10601..11812
                     /gene="thiI"
                     /locus_tag="ACG8FK_001925"
     CDS             10601..11812
                     /gene="thiI"
                     /locus_tag="ACG8FK_001925"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS5097521.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4150 a         2041 c         2527 g         3456 t
ORIGIN      
        1 aataaagctt atgcaaagaa aaagaaatcc aatcacttgg gtttcttttt cttttattat
       61 tatgatgaat caagctttct gtttttgtca cgtatgttgt aatctttacg aaagagtaga
      121 gttggctact cataatcaac catacttccc atcatcgcta aatacgaaat aaatcagcat
      181 tcctacacca atcaaagcac aagctgcacc aagaaaaggg atgacattcc caaagttatc
      241 tgtattcata agtatgccac cacccacaaa caaaaagccg ccagcaatca ggtttttcat
      301 tttattcatc tcctttctta cgttatagta tttccttctt tcaatgagta ttttgaaagc
      361 tatcttattg tatgatgttc ttctaggcct cttctttatt atacaggcca ttttcgttat
      421 ctcatagaaa tattcctttt tatctgaaaa ctattgtata ccgtatgaca tttttcttca
      481 tgaagttgta agtatattct cattcaaatg aaaatttcat catagaagag attgtatttc
      541 taaattctta agagtaaaat acgaagtata acttggattt ggaaggatga ttggatgaaa
      601 gctattacgc ctgatatgac aacaaaattt aacaaacagt ttcaagcaaa tatcaaacag
      661 caggcattgc agcgggcagt tgtgaagaac ggaattactg cttctgctga aaatgtttca
      721 gcacatgtgg agaatacacc ggtattttct atagatttgg ctacggggaa agtggctaat
      781 caaatgcaat cggggcgttg ctggatgttt gctgcactga acacattccg tcataagatg
      841 ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa attacacttt tttctgggat
      901 aaatatgaaa aggcaaatta tttctatgaa aacatcattg caacaggaaa agaggcaatc
      961 gatagccgga aagtagcttt cttactcgct actcctcaac aagatggcgg ccaatgggat
     1021 atgatcgttt cattattcca aaaatatggc gttgtgccta aatcggtgat gccggaaagt
     1081 gcgaatagtt caaattctcg agatttgaat aactacttga acaaattact gcgtaaagat
     1141 gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat tagacgctat tgaagaaaag
     1201 aaagaagaaa tgctagaaag catttataac ttcttagcta tctcattagg aacacctcca
     1261 aaagaattcg attttgagta ccgcgatgaa gaaaagaact atcatcttga ccgaggactg
     1321 acaccacaaa ttttttatga taaatacatc ggtgtaaaat tagatgatta tgttagcgtg
     1381 atcaatgcac caacaaaaga caaaccattc aatcgttctt atacagtaga gatgctagga
     1441 aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg atatggaaac tttcaaaaaa
     1501 ttagcgattg ctcagcttga agaaggagaa tctgtttggt ttggttgtga tgtaggtcaa
     1561 tcttctacta gaactagtgg catcatggcc ttagatgctt attcaatgga tgatctattt
     1621 gatacggatt ttacaatgac taaagctgaa cgtttagatt ttggagaaag tctgatgacc
     1681 catgcgatgg tattgaccgg tgttgactta gtagacgggc aatcaacaaa atggaaagtc
     1741 gaaaatagct ggggcgaaaa agttggaaag aatggcttct ttgttatgag tgatgcctgg
     1801 atggacgaat atacgtacca aatcgttgta agaaaagatt tattgacgaa agagcaatta
     1861 aatgcgttca atgaagaacc aattgttcta tctccgtggg atccaatggg tgctttagca
     1921 tagacgagta tttcaatgaa gagtatgtag taaagataaa aagagctggc ttaagccagc
     1981 tctttttatc tttaaagtcc attccctgtt ttttatggtc cctgtgctat aattctaaat
     2041 aaaggataag gattggaaat aatcctttta tcattaaaaa tatgatgacg aagtagaata
     2101 aaattgggag taggggggaa agtcatggat atccaaactg ttttatctga tctgaaatta
     2161 aatggtaaag tgattccagt tgttggtggc gatgttaatc agacctatcg tataaaaacc
     2221 gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa aaaaaggatt ttttgaagca
     2281 gaagtagacg gattaaaaga actgtcagca ttcgtacgtg taccggatac gtatatgcta
     2341 ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga tcgagccggg aaaaggggat
     2401 caacgagatc ttgctgcagc tttagctaat ctccatcagc aaacagcgcc acaatttggt
     2461 tttcgtaaag ataactattt gggtacactt gttcagaaaa attcttttga agaagattgg
     2521 tggacttttt tctttaaaga tcggcttgaa tcccagattt ctttggcaga agaaacgaat
     2581 cgatggaatg tacagcgtca agaaaagtat ctgcgattta aagaacgtgt attgaaatcg
     2641 gttgaaccaa aaaagatcac tccacgcctc ttgcacggag acctgtggag tggtaatgtg
     2701 ttctttgacc agcaagggca tccagttttt gtcgacccag ctgtttctta tgggaatcgg
     2761 gaacaagata ttgcaatgag ccagttattc ggtggtttcc gaccggaatt tttagatgct
     2821 tatcaaacta tatttccatt agaaaaggga tggaaagacc gactgccgat ttaccagtta
     2881 tattatcttt tggcacattt gaatatgttt ggagaaagtt acggttccca agtagatcaa
     2941 ttactagaaa atttttaaga ggagggcgtg tttttggcaa aaaaatatac caaaaagcat
     3001 gaagtttcat attatgagtg tgacatcaac caaacgatga catttccttc gatgttagga
     3061 gtagttatca aaacgtctga agatcaaagc gaccagttgg gaagaggcag tgaattcgtc
     3121 aatagctttg gacttacttg ggtcatcacg aattattcca tggagattaa tcgtctccct
     3181 agagtcggtg aagtaatctc agtaacgact caagccatgg agtacaataa gtttttctgt
     3241 tatcggaact tctggatcca tgatgaagat gggaaagaat tagtcaaaat agaatctgtt
     3301 ttcgtattga tggattttgt gaatcgaaaa atgagcagtg ttaatgaaga gatcattgct
     3361 ccttttgaga gtgagaagat caagaaaatc aagcgtcagg aaaaaattga aaagattgat
     3421 tcgggcttca tgttgccata tcgtgttcgt ttttatgata tcgatagtaa tcagcatgtt
     3481 aataatgcga tgtattttaa ttggatcatt gacgtattag gatatgactt tttgactacg
     3541 catgttccag aaaaagtaat tattcgattt gataaagaag tagagtacgg gaatgagatc
     3601 gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca aaacaaaaca tgaaatccga
     3661 atgcaagaac agctgtattg tgaagcaaat attatatgga aaaataaata aaaaagaggt
     3721 tgtgacagaa gggggcaact tcaagaaata agaagaaatt tacgaaaatt gtttctcaaa
     3781 tttttgtaaa ttttcgactt agttttcgaa gaagctattt ttgtcacagc ctctcttttt
     3841 aagtttagct attagataat gatgtttagt tttggataaa cgaaatcaat tcttcacccg
     3901 acaaaccata gtttacagca atcaatagtt ttaaacgtgc tttttgacta ttaatactgt
     3961 tacagaaaat aatccccatt ttttctagtt cttttccccc gccttcataa tcataaacag
     4021 gctctgcgat tccattgaaa caacgagaaa caagtactac agggattttt tggacaagaa
     4081 gcttttccaa agcagctaag gcttgtggag ggagattccc cgcacctaaa gcttcgatta
     4141 ccaaaccgtc tactttcgta tgggcgatgg cttccaataa gtccccttgc atcccagcat
     4201 atgctttaac gatcgggata gtcccatcta cagcggaaat gtccagtcgc tttgtttcta
     4261 aaagttcctg caagaataaa attcgattct tagttactaa gccgatggga cccaatgtag
     4321 gtgttcggaa agtagcaaca tttgtggtat gggtctttgt cacgtagcgt gcagaatgga
     4381 tctcatcgtt catgaccacc aaaaccccct tatctagcgc ttcttcacaa cttgcgacac
     4441 gaatcgcact ttcaaaatta taaaggccgt cacttccaag ttcattactt gaacgcattg
     4501 ccccagtcaa aacaattggt aatttgttgc caatcgttgt atcaagaaaa aaagcagttt
     4561 cttctaatgt atctgtaccg tgtgtgatca ccactccgga tatacctgat gcataagcgg
     4621 acaagatacg ttcttttaat accaacatgt gagaaggcat gatgtgtggt gagggaagat
     4681 tgaagatatc ttcaactaat aattctacat gcttggggat tgtgatttct gcatctaata
     4741 atgggtttgc cacatcagga ctaacaccgc ccgtgagcat gttttctttc atggcgatcg
     4801 ttccgccggt gtgtagtaca agtatttttt tcattttaca gacttcctta cgaataattt
     4861 tgcatagccg ttaaatctta ttgtatacta tataaaagag ttttgctatc agtgaagaat
     4921 aacgaatagc ctaacttatc ataaaaaagc tgaccttatt aaaatggaaa agactcaacc
     4981 ttgacctcct aatacaatgg agtatctggt tgagtttctt ttacaagaaa gcagccaagg
     5041 aaatcaatca cgaaggtggc cggaaatgat cagaatcgga ttaacatcgt ttaaagaaca
     5101 tgacaaatta actggtaaaa agaataacac tctttatgaa tatgcagggt ttctaccctt
     5161 agtggaatta gatactgcct attatggaat tccccgaaag tcctcagttg agaattggat
     5221 aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc tatcaaggaa tcagcggtca
     5281 aggagactgg aagccttatt atcagaccga agaagagatg attgcgcatt ttttagatgt
     5341 tatgtcgcca atggtagatg ctggaaagat gttttgcttt ttagtccaat tcccctcatc
     5401 attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa cttagaaagt ggtttgaggg
     5461 ttatcctgtt tctatagaat tgcgggatta ttcttggtac agcgatgaat ttcttgaacg
     5521 tacaagaaac tttatgtcta gagccgcttt ttctttagcc atcatcgatg agccacagtt
     5581 gctgaataca acgattcctt ttgatccatt tgtcaccaat ccgaactttg ctctttttcg
     5641 attccatggt aggaatgcgc aaggatggca agcaaatgat aaagagtggc gtaaaaaaag
     5701 aacgttatat cgttattcag aaaaagaact gcaagaacta gcacatgaag tcaaagtagt
     5761 tgattctaag acaaaagagg taggtgtgat ttttaataat aattctggtg gtgatgcggc
     5821 tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag ccagatggac tcaatccaac
     5881 ccaaatggat ctgttttaag ggcttatttt attcaagaaa ggggcgaagt ctatgatcaa
     5941 agctattttt tttgatattg acggaacatt ggtcaccaat cgttcaaaag cgttggaatc
     6001 aacaaaacaa gcaattgaat atgctcggaa aaacggcatt ttatgcggtg tagcaacggg
     6061 gcgcagccca gtaaagatca aagaaatcat tgatgaattg gaattagata tgtatgtcgt
     6121 gtataatggt caactggttt ttactgcaga tcgcacgatt atcgaccacc cttttgaaca
     6181 aaaagtattg gaacatattg ttgaatttgc agatgagaac catcgacaaa tcgtttttgg
     6241 tgcgagaaat cggttagatg gatcaacgac gatgctatta ggacaatcga tttttatcaa
     6301 gcgactagtc agctttttac cgcgtaaatt cccggtacgt ctgatgaaaa aaatcctcca
     6361 ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg ttagagatat taaaagaacc
     6421 aatttatcaa tgtatattgc tcagtccgga gtcagaacag aaaaagttag aacatcgctt
     6481 tcccgagtgt acgtttcagc gttcaaatac gtatactgta gatatcatcc caaaaggtgg
     6541 ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc gaaattgaaa tggaagaaat
     6601 tatggcattc ggcgatcatt acaatgatat cgagatgctg aaaggtgtag gaattggagt
     6661 ggccatgggg aatgctcaga tcgaagtaaa acatgctgca gactttgtga ctcaatcaaa
     6721 tgaagaagat gggatatacg aaggactgaa acacttcggt ctgatttatt agtcaatgaa
     6781 agaaggaaaa aatcagtatg gaagcagaga caccttttca aaaagtagaa acattccaca
     6841 aaacttttga tccaagaaga ccagagatac ccacggcttt tacagaaaat gaagctcata
     6901 cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt tgcagctact tcacctaata
     6961 aagaacaact gcatgaagct gttatgctgc tgcacgaagc attagataaa gcagaagaaa
     7021 aaatacttgg taaaacacag tggggaagtg atccgctggt tgaacaagta gatgcactga
     7081 cagatatcct ttactttact tatggttctt tttcattact aggaatagtg cctgatcgaa
     7141 tattcgaaat cgtccatcag gcaaatatgg ggaaattatt tccagatgga aaacctcatt
     7201 atgaccctgt aacaaataaa gtgttgaaac ctgaaaactg gcagatggaa tatgctccag
     7261 aacctaaaat cgcggcagaa atccaacgtc agattcaaga agcgattgct aagaagaaat
     7321 ccgctgatat aggcatgtgc acaaatgaaa aagagtgatc agagtttcac tatttttgtt
     7381 atctttatac gatggaaaaa caaaagattt tcgtttaagt tagtgaatgc atgaaaatag
     7441 tatgctataa tctagaatag atgtcttttg cacgagagac gtattatttg gatttatgcc
     7501 aagcagaacg ccatctgttg cataaaagat gcatactgaa aacggaggaa ccataaatgg
     7561 gaaataatgt tatcatcgga atcgtcattg caatcattat agtcgccatt gtcgtctttt
     7621 tggttagtta cttgatgaag aaaaaaaacc aagatcgatt ggatgcctta gaaaaacgca
     7681 aggaagaatt atttgacttg ccagtgattg aagaagtcga tgatgttaaa aaaatgcatc
     7741 tggttgggca aagccaaaat acctttcgtg aatggagtca aaaatggaat gacatatcta
     7801 cttcctcgtt cgctgaacta gaaagtcaaa ttttcgaagt ggaaaacctc aatgaaagtt
     7861 ttcggttctt aaaaggaaaa aaagcagttg ttgaagcaga agctacaatg gaccaaatgg
     7921 agaaacaagt gacggaaatc cgtgaaggtc taaaggaatt gcgggaaacg gaagaacgca
     7981 actcactagc tgttcagcag gcacttgacg tctatgaaga actgaaagaa aaagtcagag
     8041 aagacgagca tgaatacgga ccagctcttt ttgaattgca aaaacagatc aaagtgatcg
     8101 aaaaagaatt tacgcagttt gtgactttaa acacttctgg agaccctgtt gaagcccgcg
     8161 aggttttgga taaagcagaa aaacatacgt atgaagtgga agctctgatg aaaaaaattc
     8221 caccgctgta tgaggagctc cataccactt tccctgaaca acttgaagaa atcagtgata
     8281 cttacgagaa attgatggaa gaacaatatg tattcccaga agaagatctt gctgaagata
     8341 tcgcaaaagt aagtcgaaga attgaaaatt cattagccaa tcttgaaaaa acagaagttg
     8401 aaacggtcga atttgaaaac cgtgaaacag ctgatttgat tgattcgtta tatgatatct
     8461 tagaacggga aatggaagct cagagatatg taaaaactaa ccaatcaacg attgcagagt
     8521 atattaaaca tacaacaaag aataaccgtc aattattgat tgagctggat cacacatctc
     8581 aaagctatac attgaaccac aatgaaattg gccgtgtacg aggtttccaa accgaagtgg
     8641 aagaaatgga acgtcaaaat gaacagatga ttccacagat ccgtcaacac gaaattcctt
     8701 attcagaagt gcgcacgttc tataaaacag tcttcaaagt cttagaagat atcgaaaagc
     8761 aacaagtaga gatcgacgac tcattacatg aattgagaaa aggcgaaaaa gaagcgcaag
     8821 aaaagatcga tacattcgaa ttcaaattgc gcagtttgaa acgttttgta gaaaaacaac
     8881 gtttgcctgg cttgccgaat gattacctag aattcttttt tgttgctact gaccgtattg
     8941 aagaactaag tgttgtcttg aataaaattc gagtaaacat ggaagaggtc aatcgtttag
     9001 ttgctctatg tgaagaagat ctagaattat tagataagaa gacacatgat ttagtagatg
     9061 cagcagcatt aacggagcaa atgctccaat atgcaaatcg ttatcgtcat acacacgaag
     9121 aagtgagaga agcaattgat aaaagctact atttattcaa taaagaatat cactatcaag
     9181 aagcattaga cgaaatcggt acagcattag agcgagtaga acctggtgct ttcaaacgca
     9241 tcgaagattt ttattttaat catccagact tagtataaag caaaaaagtt agtaaaccag
     9301 caagagcaag acggattatt gtcttgttct tgtctttttt tgtatatttg aaaattgaaa
     9361 caagtgaaaa tttcacctgc tcttgaaagc accttggaca accgttataa tagaaaagaa
     9421 tttcaaaaaa gaggggtttc ttatgatata ttttgataac agtgcaacaa ccccaatctt
     9481 tccgcaaagc ttagatgcat atgtaaaaac cagtcagcgg attattggga atccatcaag
     9541 cttgcatgat ttaggcaatc aagctaaccg gttgttgcag caagcaagaa agcagatcgc
     9601 tgatttgatc catgtagaac cagaagaaat ctattttact tcaggcggaa cagaaggaga
     9661 taactgggta ttaaaaggaa cgatgatcga aaaaagagaa tatgggaatc atatgatcat
     9721 ttcaggagtc gaacatccgg ccgtcagcga gacagcagaa caattaaaag aattaggaat
     9781 cgaagtaagt atcgccccag tggataaacg cggatttgta cgtgtcgacg aattgaaaga
     9841 attaataaga aaagagacag tattagtctc tgttatggct gtgaataatg aagtaggatc
     9901 gattcagccc atccaggaaa tcagtgatct tttggatgat tttcctaaaa ttcatttcca
     9961 tgtagatgct gttcaggcga ttggaaaagt accattctcg caatggttga cagaaagagt
    10021 tgattttgca acattttcag ctcataaatt ccatggccca cggggaactg gtttcattta
    10081 ttggaaaaag ggtcgtcgtt tagcaccatt actgacaggt ggagggcaag aaagaaatca
    10141 gcgaagtggg acagaaaatg ttccaggtat tgtggctatg gttaaagctt tacgtttatc
    10201 attagagata aaggaacaaa aacctgagca gacacaacgc ttaaaagtag ctctaatcaa
    10261 tgcgttgaag acgtatgaga aagtgacgat tttttcagaa ggaactgact atgcaccgca
    10321 tattttatgt tttgctctaa aaggaattcg cggcgaagtt ctcgtccatg ccttagaaga
    10381 aaaacaaata tttgtttcta ctactagtgc ctgctcctca cgtaaacata tggcaagcag
    10441 tacattgcac gcaatgcatg taccaaatga tctggctaca tctgctgttc gaatcagctt
    10501 agatgaaaac aattcgcttg cagaagtcga gcaatttatg attattttca agcagcttta
    10561 taagaaattt tctgcaatca attaaaagag gagtgttctt gtgaaataca gtgaaattat
    10621 ggtccgttat ggggaacttt cgacaaaagg aaagaatcga aaaagcttta tcatgcaact
    10681 agcacaaaat gtcagacaag ctttgaaaga ctttccagaa atcaaaatcc atgctgatcg
    10741 ggaccggatg catcttttat tgaatggcgc agacgggcag ctagttattc caaaactagc
    10801 aaaaatattt gggatccaaa atttctctcc aagtattcgt gtagaaaaag atgtcgaagt
    10861 tttgaaacaa gctgttcaag acatcatgaa agaaatttat actgggaaag aaacatttaa
    10921 aattatcgct aaaagaagcg atcaccaatt tgaattgaca agtaatgaat tgaatcaaac
    10981 attaggtaat gctgtttttg atattttccc gcatatccaa gttcaaatgc gtcaaccaga
    11041 tattccatta cgtgtagaga ttcggcgtga tggcgcttat ctatcgtatg aaacgatcaa
    11101 gggagctgga ggattgcccg taggaacaag cggaaaagga atgcttatgc tttctggggg
    11161 gattgattct cctgtagcag ggtactttgc catgaagcgg ggagtggaaa ttgaagctgt
    11221 tcattttgct agtccgccgt atacaagtga acaagcactg caaaaagcaa aagatctaac
    11281 agccaaacta gcaccttatg tgggaacgat ccattttatc gaagtgccat ttaccgagat
    11341 tcaagaagaa atcaaaaagt cagtgccaca aggatattgg atgacaatta ctcgtcgaat
    11401 gatgttgcgt ttgaccgatg aaattcgccg tattcgtcat ggtttagtca ttttgaatgg
    11461 tgaatcatta ggtcaagtgg cttcacaaac tttgcaaagt atggtggcta tcaatgaagt
    11521 aacgaatacg ccaatcattc ggcctgtagc gacaatggat aaacttgaaa tcattgaagt
    11581 agcagaacag atcgatacat ttaatctttc tatccagcct tttgaagatt gctgtacgat
    11641 ttttgctcct ccacagccaa aaacacgccc tcaactggaa aaggtccatc aatatgaaga
    11701 gcgtttagca atagatgaaa tgatccaacg agcactagct ggcttgaaga tggaagagat
    11761 acaagcagaa tcgtctaatg aaaaaactga agaattttca gaattgctat aagaggttat
    11821 gaaaatagtg ttttgaccag agtattagag aaacaaaacg aaaaaatagc tccatgtatt
    11881 ttaccgtttt tgaactaatg tcgaagatta gctgattgaa taccatttat caagaagtta
    11941 tggaaaagcc ggttcttttt tactaactga aaaataaaat tttatttaga gatcaagcta
    12001 cagttgataa atgatataaa aaagagaatt tcccatgaaa tagcgaggtt gtgacagaag
    12061 tgggcagctt caagaaataa gaaggaattt acgaaaattg cttttcaaat ttttgtgaaa
    12121 tttcggctta tttttcgaag aagttacttc tggaacaccg tgtatcagag gttg
//
LOCUS       DAZUMV010000083        12163 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000083 DAZUMV010000000
VERSION     DAZUMV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.083"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..730
                     /locus_tag="ACG8FK_001926"
     CDS             158..730
                     /locus_tag="ACG8FK_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS5097522.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            875..1579
                     /locus_tag="ACG8FK_001927"
     CDS             875..1579
                     /locus_tag="ACG8FK_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5097523.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1807..2145
                     /locus_tag="ACG8FK_001928"
     CDS             1807..2145
                     /locus_tag="ACG8FK_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097524.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2130..2984
                     /gene="lgt"
                     /locus_tag="ACG8FK_001929"
     CDS             2130..2984
                     /gene="lgt"
                     /locus_tag="ACG8FK_001929"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS5097525.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3131..3364
                     /locus_tag="ACG8FK_001930"
     CDS             3131..3364
                     /locus_tag="ACG8FK_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS5097526.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3562..5466
                     /locus_tag="ACG8FK_001931"
     CDS             3562..5466
                     /locus_tag="ACG8FK_001931"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5097527.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5776..6138
                     /locus_tag="ACG8FK_001932"
     CDS             5776..6138
                     /locus_tag="ACG8FK_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFS5097528.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6155..6766
                     /locus_tag="ACG8FK_001933"
     CDS             6155..6766
                     /locus_tag="ACG8FK_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFS5097529.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6868..7776)
                     /locus_tag="ACG8FK_001934"
     CDS             complement(6868..7776)
                     /locus_tag="ACG8FK_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5097530.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7818..8132)
                     /locus_tag="ACG8FK_001935"
     CDS             complement(7818..8132)
                     /locus_tag="ACG8FK_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS5097531.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8431..10362
                     /locus_tag="ACG8FK_001936"
     CDS             8431..10362
                     /locus_tag="ACG8FK_001936"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5097532.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10655..11206
                     /locus_tag="ACG8FK_001937"
     CDS             10655..11206
                     /locus_tag="ACG8FK_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5097533.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11519..12061
                     /locus_tag="ACG8FK_001938"
     CDS             11519..12061
                     /locus_tag="ACG8FK_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFS5097534.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         3952 a         2034 c         2555 g         3622 t
ORIGIN      
        1 aatttaatag agaagcctgg cgtctattaa gaaataattt tcttgatgag cgtcaatttt
       61 tttaacggaa atctatccta tactaaattc agtaaagaaa aaggaggaat caaagaaagc
      121 tattttaaac aaaccaaaat tataggagga ataaatcatg actgaaaata aagctactca
      181 agaaagaaca ccccaagaaa gattgcaaga agaacaagaa cacaaggaac atgttcatca
      241 tacgaaaatt aatgcagcgg ccattgcgga tcatctttta ggcaacatgc atacattaca
      301 tgtgaaactg caccaatatc actggtatgt aaaaggagcc aatttcttta cgttgcatga
      361 aaaatttgaa gagttgtata acgaaaatga aaaatggttc gacaaacttg cggagcgtct
      421 aattacttct gggcataaac ctgcttcgac aacggttgaa tttgaaaaat attcgatgct
      481 ttcggaagat gcagttaata aatacgctaa agcagaagac atggttgaaa atattatcga
      541 ggacttcaga agcactcgtg acctaactat tcgcgcgatt cgtttagctc aagacgaggg
      601 tgacgatgct ttagaagata cattgattgc ttttaaaaat gacttagaca agaacatttg
      661 gatgctgcaa gcgttcattg gtaaagaagc attagaagat gatgacgctc ttgatgagga
      721 tgaagattaa ggtaaaagta gaagctgatt tagggattgg aattctttcc agtccttttt
      781 ccattttctt gatgtgcatc aatttttttg tagtaagaga cttatataat aaaagcagcg
      841 aatataaaca aaataaataa agggggagaa tgaattgact aaacataccc atcatgaaat
      901 tggcgaccac gcagcctgta tccgcttggt tccaatattc aatcacttag aggatagcgc
      961 gatgaattac attgctgaaa aagcccacac gaaacagtat caaaaaggag agtttctgtt
     1021 tcgttcacaa gaaaaagagg atactttata tattatcaat cgtgggaaaa tccgtattta
     1081 tcgattggca gactctggca aagagcagct agtgcggatc ttgaaccctg gtgatttcac
     1141 cggagaatgg accttgttca acccggatgc agtacacgag gaatatgccg aagcaacccg
     1201 agataccgtt gtctgtatga ttcagcaaaa agatgtccaa gattttctgg aacaataccc
     1261 agctatttct ttaaaactat tggcggaaat gtctaaacgg ttagaaagtt cagaacgtca
     1321 aacgacacaa gtagccgtgg agagtgtcat tacccgttta gttttgtttt tggcagaaaa
     1381 tgtggaacct gaaatgggca actctcccac catcaccctg ccgatggcaa aaaaggacat
     1441 tgcttcctat ttaggaacga cacctgagac catcagtcgc aaatttggag aattggagga
     1501 cgaaggattg attcaacagc tgtctgggaa aaggatcaag attcaggatt tagatgattt
     1561 attgctttac agcgaataat cggacacact tttttctaca cgttggtgtt ctgtcgctct
     1621 tcattaacaa cgggacagaa ctcagtgctt ttgcttccat ctgtccgttt ttggcttttt
     1681 taagacagaa cgaccttcat tcattggaaa aataaaagca caaacatgat aaccctcaag
     1741 tttttttgtt ttatttttct ttaagatagg gttgtcgaac aaaacgtaaa aataagtagg
     1801 gtgaatgtgc attgtttttg ggtgatagtt gccatcacgt tgtcagcagc gggggcattc
     1861 tttgtcgtcc ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt
     1921 gaggcagata ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc
     1981 ggacacggaa aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact
     2041 gtttggtttc catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg
     2101 cgaaaaattg aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc
     2161 atgtggtgga gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca
     2221 tattagcggc tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata
     2281 ccattatcga tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt
     2341 atgtgttatt tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga
     2401 atggcggcat tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg
     2461 caaagaaaaa aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt
     2521 tagcccagtc cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg
     2581 ttactcgtca attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata
     2641 tgaatggtac ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct
     2701 ttgtcctcat cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt
     2761 tgagttacgt tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca
     2821 gtttatggat tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg
     2881 gcgcgattgt agtatggttt atacgcagac aagattatcc accaatttct tattattctg
     2941 atggcaataa ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag
     3001 aagtgcaaaa cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg
     3061 taaagaggaa aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga
     3121 gataggaatt atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat
     3181 gcaaaaaatt gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt
     3241 attattcaac tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga
     3301 gattcaaaaa gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt
     3361 ttaacagatt aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt
     3421 ttttgttgca atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga
     3481 tgtcagtcaa ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa
     3541 ttaaatttgg agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc
     3601 gtcatcagtg gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta
     3661 ggactttcag agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt
     3721 caagcgtttc aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca
     3781 gcgattggtg cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc
     3841 ttattcggac actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa
     3901 ttgactgaaa tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa
     3961 gtagaagtgg atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt
     4021 ccagtagatg gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt
     4081 gaagctattc cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa
     4141 aacggaacga ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt
     4201 attgaactcg tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc
     4261 ttttctaaat actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt
     4321 caaaatattg agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc
     4381 ggagtgcctg tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta
     4441 aaagggagtg aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca
     4501 ggtactttaa ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct
     4561 gctgaaacgc ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa
     4621 gcggtcttaa atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg
     4681 aaaggcggcg gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc
     4741 ttgatggaaa aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt
     4801 gaacaacaag ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg
     4861 ggcattcgcg atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg
     4921 ggcgtgaaaa atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc
     4981 ggcgaacttg gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat
     5041 atcggaaaac ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat
     5101 agtccgtcct tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg
     5161 attgaaacat ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta
     5221 ggattagtaa aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta
     5281 gtgttggtct tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg
     5341 ttggttcatg aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat
     5401 aagttgaaag gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata
     5461 tcttaagaat aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg
     5521 aaactcagat ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga
     5581 gtatagagta tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat
     5641 aaaatttaaa ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat
     5701 taaggtagat tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa
     5761 tgtgaggtga gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta
     5821 aatgtaataa aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     5881 tgtttcagcg attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg
     5941 tgtgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     6001 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat
     6061 tctttagcga atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat
     6121 ctatctatga aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat
     6181 cgctgtttat atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca
     6241 aagccacaca aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat
     6301 tttggtagcc gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat
     6361 cattggtctt ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga
     6421 agaggaggaa gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt
     6481 attctggaca gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat
     6541 tccatacttt acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat
     6601 ctctgttgcg gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga
     6661 aacgttagaa aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt
     6721 tattatgata gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg
     6781 aaagataaaa agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa
     6841 tgaagtattt tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc
     6901 ttctttgctt gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac
     6961 ttctgcaatt tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac
     7021 cgtgatgata agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc
     7081 ttttacagca ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg
     7141 cgaaccattc aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat
     7201 ataaagtgca atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat
     7261 tagaatactc acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt
     7321 caacatgctc tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa
     7381 tgcaatagct acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat
     7441 caaacgtggc acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt
     7501 tatcagcgcg cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt
     7561 tttctctgaa taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc
     7621 atgaactgcg tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct
     7681 tatagagaaa ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg
     7741 attcgagtga ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg
     7801 ctacatacta attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga
     7861 atactaaaaa catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat
     7921 ttcactaagc gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg
     7981 tccagagaaa tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc
     8041 gacaatcgtg taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct
     8101 ttatcgattg gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt
     8161 ccgttcatat ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag
     8221 aagtggcgtt ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac
     8281 atatgattaa ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga
     8341 aaggagtcag aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg
     8401 aattataaat tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa
     8461 gaaaagcata atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc
     8521 gttttgtact ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat
     8581 cagttggtac aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt
     8641 cttcttgaag gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct
     8701 aattctcata ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg
     8761 gaaggaacat tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa
     8821 ggaagaagca aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta
     8881 atcctgcctg atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa
     8941 cttcaagtgt tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca
     9001 tccattgatg agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat
     9061 ggcgtcttcg ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa
     9121 aacaaagata cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa
     9181 acaaaagctg cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt
     9241 gccattcctc tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt
     9301 gtctatagag gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact
     9361 gtctcggcaa cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt
     9421 agttcttacc tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc
     9481 ttgaccaaag gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa
     9541 aacatgattg atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct
     9601 attctaagaa agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt
     9661 aaaggattga cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt
     9721 gatgatgttt cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca
     9781 gtcgtatacg tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg
     9841 agtgaatatg ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta
     9901 attactggtg actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa
     9961 gtgattgcga atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa
    10021 tacggcgtga ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca
    10081 gatgtgggaa tcgctatggg agatgggact gatgtggcag tagaggtatc tgatttagtt
    10141 ttaatgcaaa acaatttatc taaattggta caatctcata atatttcttc gaaaatgaac
    10201 cgtgttattt ggcagaatat tattttttca atggccgttg ttgctttttt aattgtcgtt
    10261 agtttcttag gcttaacgga tattgcaatc agtgtcatta ttcatgaagg aagtactttg
    10321 gttgttattt tgaatggact tcgattgcta aaggcagtct aaagaatgat tgcacaactt
    10381 caaattaatc gagtaataga gataattaag gattacacta cattttttat ctctattact
    10441 tgtttatatt tatctagatg ttcaaagtca aaatgatata tcaaccatac tcttgtggcg
    10501 cgggttgaat ataagataca tgaaattgga taaaatcatg tatcaagaat cctatcctta
    10561 tagggttctt tttttttact ccccctaaac acacgatttc gtgttgtaat aactataatc
    10621 atgtattcga acagactaaa aaggagggat tgtcatgggc tataatgttc agccactacg
    10681 gtcgcaacag gagattaatg attttttatt ttgcctgcga cggaataaaa atgcggaacg
    10741 tgacgttttc ttgtttctaa ttggaattaa cagcggtctg cgcatgtccg acatcgttaa
    10801 actcaagaaa aaagacgtga ttacctcaaa aaacccgcgc atcgtggagc aaaagacagg
    10861 gaaaacacgc attttatact taagcagctt acaggacttg attcacggct ataccgcacc
    10921 cttagatccg gaggcttact tgtttcccag tactaagggc ggccatttag aagtcaatac
    10981 ggtctaccag atgtttcaaa aggtcgcgaa gctgttagga agggacgata tcggcaccca
    11041 caccctacga aaaacctttg gttaccacta ttacaagaaa accaaagacg tggccacctt
    11101 aatggaaatc tttggtcaca gcagcgagaa gattaccaaa cgctacatcg gaattaatga
    11161 agatgaaatc agtgaaacgt tattaaattt tcggttaggt ttttgattgt attatataat
    11221 agataagcac agtaagacgg ttataatcac ccacagtatt ggacaaatca accttttttt
    11281 ggtatattat atatacaaat gagagcaggc tcatagtcaa cttgatataa atccggaaca
    11341 taagggtccg gcgggtggcg agcaccaaaa ctaaactcga tacaaatccg gaacgtaagg
    11401 gtccggcggg tggcaagcac caaaactaaa cctgtacaaa aggctgctct tttgagcagc
    11461 tttttttatt gggaaaaagg agcattcttc gcacgcggca aaaaaagaat acaaacgcat
    11521 gtctataaaa gagacaaccc aaattaccag acagttaaac gcgatttata aggctgctca
    11581 tctcttacaa gaacattttg tagataaaaa agtatccttt gtcggagaag tctcaacggt
    11641 agcgattatt ttttcgacaa ctaattttat gcacctctgt ggcattgatt atcgcagagg
    11701 cacacacttg ttttttcaag atgccttaga caggaaaata aatttacagg atattcaaat
    11761 caaaacggat gggacgacct ttcagaaatt acaagtaata ggtagccttg atttattatt
    11821 ggggaagcat atttctattg ttggcagagg tgtttactct tctcttcgct atgatgcagc
    11881 tattcgaaca aggaaaaaaa tattggctct gtctttgaaa caaaacggat taatctatat
    11941 ccctatctct ttgttaaacc tatcatcaaa ggaaattgga cctggacaaa aagttactgg
    12001 catttttagc gaggatttaa catccgggga acttaaaatg attatggaag tcatcgatta
    12061 atcattaata gtgatatgcg acgtcatttt gaagtaatta ttctattttg ggttataaaa
    12121 taaagcattt taagacgatt tcaaatcatt catgtgggtt att
//
LOCUS       DAZUMV010000084        11680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000084 DAZUMV010000000
VERSION     DAZUMV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.084"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..2304)
                     /gene="glyS"
                     /locus_tag="ACG8FK_001939"
     CDS             complement(229..2304)
                     /gene="glyS"
                     /locus_tag="ACG8FK_001939"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS5097535.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(2306..3223)
                     /gene="glyQ"
                     /locus_tag="ACG8FK_001940"
     CDS             complement(2306..3223)
                     /gene="glyQ"
                     /locus_tag="ACG8FK_001940"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS5097536.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3607..4419)
                     /gene="recO"
                     /locus_tag="ACG8FK_001941"
     CDS             complement(3607..4419)
                     /gene="recO"
                     /locus_tag="ACG8FK_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS5097537.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4562..5461)
                     /gene="era"
                     /locus_tag="ACG8FK_001942"
     CDS             complement(4562..5461)
                     /gene="era"
                     /locus_tag="ACG8FK_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS5097538.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5476..5871)
                     /locus_tag="ACG8FK_001943"
     CDS             complement(5476..5871)
                     /locus_tag="ACG8FK_001943"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5097539.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5855..6331)
                     /gene="ybeY"
                     /locus_tag="ACG8FK_001944"
     CDS             complement(5855..6331)
                     /gene="ybeY"
                     /locus_tag="ACG8FK_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS5097540.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6348..8540)
                     /locus_tag="ACG8FK_001945"
     CDS             complement(6348..8540)
                     /locus_tag="ACG8FK_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS5097541.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(8564..9535)
                     /locus_tag="ACG8FK_001946"
     CDS             complement(8564..9535)
                     /locus_tag="ACG8FK_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS5097542.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10241..11620
                     /gene="brnQ"
                     /locus_tag="ACG8FK_001947"
     CDS             10241..11620
                     /gene="brnQ"
                     /locus_tag="ACG8FK_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5097543.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3181 a         2442 c         2135 g         3922 t
ORIGIN      
        1 tcttgactag ggaaattcct aatttaaaag taaataaaac agtaacaaga ttattattaa
       61 tctttttcat ttttatttcc tcacttttca tgagcgtttt gaaaattcta gcaaagaata
      121 aaataacaaa aattattaaa atagaattaa taacagcttt tatatttata acaaaaaaac
      181 agagacacag cacgtttcgt gcatgctctc tgtttttcaa acaaaagact attttgtgat
      241 gatttgatcc aagcttgcta atgacaaagc catggcagtt atttgactaa gttgtgttag
      301 tcggttctta cgtactgctg gttcatcagc catgaccatc gtttgatcaa aataattttc
      361 gatcagtgga cgaagagcga caagctggtc gtagttttcg tggatcgttt ggtcagcaaa
      421 acttgctttc attgcttcta ctgctttgtg taattctttc tctgcgtcgt tttcgaacag
      481 ctcagggtct acttctgatt gtttttcaag ctgcgctttt ttcgctaagt tcacgacacg
      541 tgtcaatgct tccattgaag gtttgaagtc ttcatctgca agatgttgtt tgaacaattg
      601 tgctgctgca aatacttttg tcagatcttt ttgttctgca ttcaaaacag cttcgatgac
      661 atcatgacgg atattcttcg ttgtcaataa ttggcgcaga cggcctttga taaagtcgat
      721 cacctgttgt tgtcccgctg acaagccaat gccaaaacga tccgtatctt tactgataac
      781 ttcactaatc gtttcttgca gaacagacaa aggaaaggcc cattttttcg attcgatgat
      841 tcgaacgaca ccatatgctt gacggcgcaa agcatatgga tcgtttgagc cacttgggat
      901 catcccaact gcaaagaaag aaaagatact atctaattta tcagcaatcg ctaaaacaga
      961 acctactgta gtctcaggta aatctccttc actggatata ggcatgtaat gttcacggat
     1021 cgctgttgct acggctggtt tctcgccttt aagcagagca tatttttctc ccatgatccc
     1081 ttgtagttca gggaattctc ctaccatgtt ggttactaga tcaaatttat agatttcagc
     1141 agcgcgttgc agatccatca attcttcctc agatagtcct acttgttcgc ctatgatgcg
     1201 agcgatgact tgtacccgtt gcattttctc atagatgcta ccgatttttt catggaaagt
     1261 aacagttttt aatttttcaa cgtattggtc gattgaagct ttcttgtctt cattataaaa
     1321 gaattccgca tcttctaaac gagcagtcaa tactttttga ttgcctcgta ccacattttc
     1381 caattttacc cgatcgccat tacgagcaga aataaaatga ggaagcaaca taccttgatc
     1441 gttccgtact tcgaaataac gttgatgttc tttcatagaa gtgactagta cttcttcagg
     1501 aacagataga tatttttctt cgaagtcgcc agaaaagaca gttggatatt cgactagatt
     1561 gttgacttct tctagtaaat cttcatctag atcaatgatc cagtgatttt tttctgctaa
     1621 ttcgtttgct tgtgtgatga tcatttcttt tcttttttgc ggtgaagcaa taacaaattg
     1681 ttcattcaat ttttcttcgt attcttttgc atgcgtaaat gtcacatctt cgcctaagaa
     1741 acgatgacct cttgaaactc tgtctgtttt gacatccaag atctcaaacg ggattatttc
     1801 gtcatctaac aatgccacga tccagtgaat agggcgaata tattcaaaat cgtattctgc
     1861 ccagtgcatc gttactggga aagtgaggct tttaatgaca tctgctaaac ctgtcaatac
     1921 ttcttttgct tctttgccgt gagtgtattt cgttacatag acatattcca cgccttttaa
     1981 ttctctaaaa gtgatagcat ctgtcgtcag accttgtccg cggacaaatc cttcagctgc
     2041 tttcgtccag tttccatctg cgtccaaagc aatttttttg gctggtcctt tgacttcttc
     2101 ttcgctgtct gcttgacgtt ctggaatgtt tgttactttg attgctaagc ggcgcggtgt
     2161 agaaaacgtt tcgatcgtat cataagtcaa ttggttttca tctaaaaatt ttgctgtttt
     2221 ttcttctaat tgtttcatgc ttggtgtgac tacatgagca ggcatttctt ccagcccgat
     2281 ttcaagtaat aagtcttttg ccatcttatt cagcctcctt cacgttttct ggatttgctg
     2341 tgtttttatt caataaaggg aatcctaatt tttcgcgttc tgccacaaag attttggcta
     2401 cagcacgagc catgttgcgg atacgggaaa gataacctgc acgctctgtt acggatactg
     2461 caccgcgagc atctaataaa ttgaatgtat ggctacattt caaaatataa tcatacgctg
     2521 gatgaacaag attttcatcg atacagcgtt tagcttcttt ttcaaaacga tcaaaattgc
     2581 ttagtagcag gtcttggtcg cttacttcaa aagaatattt agaatgttca tattctggtt
     2641 gtttgaagat ctcaccatat ttcacgccat cagcccattc cagatcataa acactttcaa
     2701 cttcttggat ataagaagca agacgttcta gtccataagt gatctcagaa gtaactggtt
     2761 tacaaggtag tcctccgact tgctggaagt acgtgaattg tgtgatctcc atcccatcaa
     2821 gccatacttc ccagcccaca ccggcacaac ccatcgatgg gttctcccag ttgtcttcaa
     2881 caaagcgaat atcgtgttct aatggatcaa ttcctaaaag acgcaagctt tctaaataaa
     2941 gctcttggat attttcaggt gaaggtttca ttacgacttg aaattgatga tgctgataca
     3001 aacggtttgg gttttctcca taacgcccgt cagctggtcg gcgggatggc tctacataag
     3061 cagcattcca tggttccggt ccgattgccc gtaagaatgt atatgggctc atcgtacctg
     3121 ctcctttttc tgtatcataa gcttgcatca gcatgcagcc gtttgatgac caaaattttt
     3181 gtaatgttaa aatcatatct tggacggtca attttttact catctgtttt gcccctttca
     3241 atttttaagc gttgctgtca ataagaatac aggaaagaaa acgacaaaaa gtccctatgt
     3301 ctgattacag acatagggac gaataatttc gcggttccac cctacttccg aataaagatt
     3361 cggcagcttc atttatcgta ctcgcaagtg cctcttcaaa aatcgtttgg gtccggcttc
     3421 cactctcccg aactcgctaa accacgtgtt ttttactgat cttggtcaat gtacgtattc
     3481 attttgcagt tttctggttt gtataataga tcctctcagt gaacgatcta tttaatctac
     3541 ggttaataat agacggaagt ccctgtattt gtcaaatagt tttgattttt ttgttgtatc
     3601 tttcatttat tcttcttttt caggtatctc tttttcaggc atatttttct ctggcgtctt
     3661 taatacgtgt gcccaagact tcatttgatc aatgaacttt ttgcttttta gatgaatacc
     3721 gacatattca tcatacaact ggtcaatgag gtcacggatt gcctgcttcg tttcttcttt
     3781 caagtggatt ccactgatct tatcataaga aatagccgaa aacattcgaa cgaaataaac
     3841 agcgcgagga tctgcatgat aacggtgttc gtccattggc cagtgtttct cgcatatcac
     3901 accgccatat ttggaagaat agtcaaattt tccttttgtt tccccgcata cgacacagtg
     3961 cgtccaaatt ggcgcgattc cgaagcgttg cagtatttgc acttcaaaga tattcgtgat
     4021 gatttctgca tcatctcctt ggtccatccg ctgcagagct tgttgcgtaa attggaataa
     4081 atgcgggtca tattgatgat cttcgattgc tgcatctact agatttagca aatacgtacc
     4141 ataaccgctc aaaaagatat cttcttggat gttccgaaac gcatggacgt ctttgacact
     4201 gcttaaaaac gacaaacctt cacttcgaaa attaccgata tagactgctt cggtaaaggg
     4261 gagcaaagcc ggagctaatg gctgattttg ccgatggatt cctttgatat aaaacatcat
     4321 tttgccagaa gattccgtaa agattttgac taatttatct ttttctttgt aattacggct
     4381 agataaaatc aagcctttcg attctacaag ctggctcata ttctccctcc taaaaaataa
     4441 atctggaaaa gcagattctg ccgatccaga ttatttatgt ttcgatcttc cgctgttttt
     4501 ctcaacgtac ggatttttat ccagtttatt tctataccta acctataaga agtccagaat
     4561 atcagtactc gtcttttcgg tagccgaagt cttgcagata gacttttttg tctcgccagt
     4621 ctttttggac tttgacccaa agttctagaa atactttgtc tcctaaaaga ttttcgatat
     4681 cctttcttgc tttcgttcct acttgcttca gcatttttcc gcctttgccg ataatgattc
     4741 ctttttggct atctctttcg acgatgatcg tggcctggat atggatttta tcattctcgt
     4801 ttcgcttcat cgaatcaaca acgactgcga cagaatgagg cacttcgtct cgtgttaaaa
     4861 acaagacttt ctcacggatc aattcagaaa cgatgaagta ttcaggatga tcagtcactt
     4921 gatcatctgg aaaatattgt gggccctctg gcatttcact gactaataca tccattaagc
     4981 gttcgacatt gtttccttct gtagcagaga tcggaatcac ttgtgcaaaa ggcatctgcg
     5041 tcgaataatc ttcgatgatt cccagcaact cgtctggatg gatcttatcg attttattga
     5101 taatcagata aacaggagaa tgcacgtttt ttagacgttc aataataaaa tcgtctcccc
     5161 ggccacgttt ttgatcagca ctgatcataa ataagactgc atccacttct ttcaacgcac
     5221 tgtaagctgc ttctaccata aaatctccta gacgatgctt tggtttatgg attcctggtg
     5281 tatcgataaa aatcagctga gcttctggtg tagtatatac tccttggatt ttatttctag
     5341 ttgtctgtgc cttgtcactc ataatggcaa ttttttgacc cacgattcgg ttcaataatg
     5401 tggattttcc tacattcggg cggccgacga ttgcgacaaa ccctgattta tgttctggca
     5461 tatacgttct cctttttaat taaacaacaa atgatatatt ttagggacaa ataaaatcaa
     5521 cccaatgatg acagcaaaac aagcggtcac taaaactgct ccagctgcca tatctttgat
     5581 ttttttccca atcggatgaa aatgaaaatc cgtaaacatg tccactacat tttcaaaaac
     5641 agtattgatg atctctacta tccaaacgag aaaaacagct aaaagcagcc ataaccactc
     5701 tatccgatca agctggaaaa tcacgcccat aaataaagca aggataccga acgcaacatg
     5761 tttcctcata ttgcgttctt cctcaaaaac ggttttgact ccttggacag caaattccac
     5821 agacatcagg aaatgtttgt ttttctctac gtttttatct tttgagtcca taggcatcca
     5881 agatctcttt ctgtaaacca aacatttctt tctcatcttc tggtgtcata tggtcatacc
     5941 cgttgatatg caaaaatcca tgtaccgcta aaaagccaag ttcccgatca tatgaatggc
     6001 cgtaagcttc tgcttgttca gcagcacgtt caatagagat catgatgtct cctagtacac
     6061 gaggtaatgg attctctcca tcttcaaaaa tcactgggat ttcgtcttcg ccttcttctt
     6121 ccagagcaaa actgatgacg tctgtcggtg catcttttcc acggtaatca cgattgatta
     6181 cttgaattgc cgcattgtcc atgaaagtaa cagacatttc tgtttcctct ggcagcttta
     6241 aataagatgc tgcaaattgc agcaagtcat cgatttcttt cattttctct tctgagactt
     6301 ggtttgtttc atccataaag gtaatatcca ttatacatcg cttctttcta ttcttcttgt
     6361 acttccttta tcttctcaac ttctgttttc attttaggat atttgatccg agaatggaag
     6421 gtgctactta aaccgctgat taatgatttt tccacttttc ggatttcttt caaggtcagt
     6481 ccactatcgt ctaattgacc atcggaaata cgctcttcga taagattatg gacaaaactt
     6541 tcgatttttt cgctcgtcgg atgatccatt gcccgtactg cagcctcaca actgtcagca
     6601 atattgacga tccctgcttc acgactttgc ggttttggtc caggataacg gaattgctct
     6661 tctgttactt ccggattgcg ttctttcgct ttgatgtaga aataacgcat tagcgtggtt
     6721 ccatgatgct gtttgcaaat atcgatcacc atttgcggca tattataatc ttccaatatt
     6781 ttggctccat ctgtcacatg tccaaagata atatgtttac tgtcttctgg caataagaaa
     6841 ttgtgcgggt tttccgctcc tgaaggcaag ttttcaacga aaaagctagc atgtttgatc
     6901 ttgccgatat catgataata gcaagctact cgagttagca aagaacgtcc gccgatttct
     6961 gctacagcgt tagcacttag attagctacc atcatactat gatgataagt acctggcgct
     7021 tcttccagca actgtttcaa taaaggatga tttggattgc tgagttcatt taatacaatt
     7081 acactgtcat cattgaccat caattcaata tatgggtgta gaccgatcgt cagcaagaat
     7141 gagaagagtg caccagtgaa cccgcagacg agcattaacc aagtcgttgc atcaccaaag
     7201 ctcattcctt gatagacgat caaaatgaca tccatcatca caggaaaaat aatgacccac
     7261 atggcagcag ataatccttg ttctgagatc cgtttgcgtc tgacaatcgt tgccatgagc
     7321 ccagagaaaa cataagacat caagataatc gttagaaagt tcgtgccaat cgaatcataa
     7381 aaaatgaaaa gtgcggatac tgcttggaaa ataccagcca tgatccctgc tcttcgattc
     7441 aagaaaaagc ttaaaacaag tggtgcaaaa gcagcgggat aaaacagcgg gatataagta
     7501 gcttgttccg tttggaataa ttggaaaaat ttcatcagca ctatgctgac tgacatagct
     7561 gtcacataga aaagcacgta ttttgttctt tctcctgcat tttggaattg aagtgaatta
     7621 taaatcagta cgccgatttg aagcaaaaca gctaaaacca tggcaattag cggaaaaatc
     7681 gaagttgttt gattagtcag tcctagtaat tccaattttt tcatagctga agcatcgatt
     7741 tgattacctt cacgaacaat gatttctcct tggaagatca tcactggttg tacagcttct
     7801 cgtgcggatt gtttcaattc ttctgtcttt ttctcattca atacgtcatt cactacgata
     7861 ccttgatcca ctaagtagcg aatcatttgt tgttgtgctg gtgtgacatt caaatattgg
     7921 atttgctctt ctgcttcttg tttaaaagct tccaattcac tttcacgaac ttgtttgctc
     7981 atctgttcgt caatgagctt cagactctca tccctcacag tatgaatctc agtttcagtc
     8041 atttcaaata tcgtcgaata aaaattgttc ggtagttttt gataaaacac aacattttct
     8101 gcattcacat tttcaaaatt tttcttcagc gcggcgatcc gctcttctgt agtaggcttt
     8161 gggatatttt catcttcctt tgccttatca atcttttcgt cataagattt gttgatggca
     8221 tcattcgtct tatcgatcaa ttcaaatagt tgcttgatcc gattgttctg gtcatctgca
     8281 agatcttttt ggtaagtgta ttctggagtg actgcttctg ctgccagttt tcttttttgt
     8341 tcggtttctt ccgtgttctc gatcgttttg ttcgcacgga tactttcttc tgctacttgg
     8401 ccttctctga aatcattggc cttttgtttg acactactaa atgtaacgcc gcagataatg
     8461 ataaaaaaca agaacagcag gaaaggcaat gctgcctttc caagtttttc ttgtagtttc
     8521 tgcagtgttt tattcaacat gtgcttatcc tcctaagcaa agcttattct tgatggagat
     8581 ctgccttttc ataagctttg atgatttcgg caactacagg gtgccgaaca acgtcacttg
     8641 cttcaaaatt aacaaaatcg attttcttga tttgctttaa tgttcgttct gcatgaacaa
     8701 gaccacttgt cgtccctcga ggcaagtcta tctgacttgt atccccattg acaatcattt
     8761 ttgattgata accgagacgg gtcaagaaca ttttcatctg agcgactgtc gtgttttgtg
     8821 cttcgtccaa gataacgaat gcatcatcca atgttcgccc ccgcatgtaa gctaaaggtg
     8881 cgatttcgat tacgcctcgt tccatcaatc ggttcgtatg atccaatcca aaaatttggt
     8941 ataaagcatc ataaacaggc cgcagataag gatccacttt ttcttttaga tccccgggaa
     9001 gaaagcctag attttcacct gcttccacag caggccgcgt caaaatgatt ttttgcactt
     9061 cacctttttt caaggcagcg attgctaata ctactgcaag aaaagttttt cctgtcccgg
     9121 ctggtcctac gccaaatacg atatcatgtt tggctaccga ttcgacatat ttcttttggc
     9181 cgctgttttt tacccgtata ggttttccat tacggtcttt gacgatctct tcctcataca
     9241 tctcaacaaa ataatctaat gtaccttttt gccccatttt caatgccgtc acaacatcgg
     9301 gcgtacttac gtggattcct cgtttgatca attcctgtaa tgtccgaatg actgaggcag
     9361 ccaaggaaat ctgggattgc tcaccaatga tctgaatgat ttctccacgt gtatggatgg
     9421 ttgtttccgt tgtttcctca atcagtttta tatgcttgtc gtgtgcacct aaaagcatgc
     9481 tgacatcatc agcagccgtc aatctaatct ctaaagaact agactcttcc gtcaaaaata
     9541 gacatcccct ttttttcgat taattctttc ctattttacc atactcagaa gaaactatat
     9601 agcaaataag aaaaggttct aaaagagttg cttaaccata agtaccaaga aacgttaaaa
     9661 aactagccgc ttccattctc taaaggtctc gtttgaataa aaaaagctga tcatgtaagc
     9721 tatgcataag atagaggaca agtattccgt ctaacttagc aagtattctc tgggtatttt
     9781 tcttgtttca aaaaaagaaa acaaaatatt tgaaatattc ttgcatttca aaaaaaacag
     9841 gtgtattata acgtcaacaa ataaatatac agcaaagcgt cgaggaaaac agtaggctat
     9901 tcagtgattt attcccagtg agtcatgtaa gtgagaaatg acaataaatc gaatagacga
     9961 acatcatttc cgagtcaatg tcgaaaacgt agatcattgt ggctgcctcc attatcaggc
    10021 tagcggataa ctttatcgaa aagcgtccgt gaatgaagaa gcttttttaa gcgaaaaaag
    10081 gtggtaccgt tctagaatct agagcccttt tcccaagtgg aaaagggctt tttttatatt
    10141 ttaaggtggt ggttttcatg gaatctgata ttagtggtat ttctagtttc tgcaacgttt
    10201 attttgttac tcaaaatgat aattattgga ggaaaaattt atggaagaaa caaaaactta
    10261 ttcatggaag caattattga tggtcagttc acttatcttc gggatgttct tcgggtcagg
    10321 taatctgatc tttcctgtac acttaggtca aatggctgga tcaaactgga tcagcgctgg
    10381 tgtaggattt gccatctcag gcgctctttt cccattatta gctatcttag ctgtagtagt
    10441 gacaaaaagt gatggtctgt atgacttagc taaacctgtc ggaaaatggt atgctgcctt
    10501 gttcttgatc cttgttcatt taacgatcgg accattcttt ggtacaccgc ggacagcagc
    10561 aactgctttt gaaatggctg ctaaaccctt tttgcctgaa aaatatgatt cattaggtat
    10621 gtttttattc tctgctcttt tctttttact tgcttatttc cttactgtac atccaacacg
    10681 tttggtcaaa tatgtcggaa agtatttaaa ctccttgttt cttgcattat tagctatcat
    10741 ttttgtcgtt tctttattcc aacccatggg gaatctaaac caatctgctg cagattctta
    10801 tcagacgaac gctttgacaa atggcgtttt agaaggatac aacacggttg atgcagtagc
    10861 tttacttgct ttaagtgtca cttttgtcca tgcagttcgt gatcttggtt ttaaagataa
    10921 aaaagtcaca gaattaacag caaaagctgg tagcctgagc gttgcgttag aagtcatgat
    10981 ttattttggt ctggtccttc tcggtgcgat gagtttgaat aagatgtctt tatcagaaaa
    11041 tggcggaact gcactttctc agatcactaa tttttattta ggtaacttcg gtgctagctt
    11101 cttaggggta atggtcacat taggtgtctt cactactgct atgggactcg ttgtttcttt
    11161 tgcacaagat ttccataaac tgttccctaa agtcagttat atgacatggc tgcggttaac
    11221 gacttttgtc tcttttgtcg ttgcgaatgc cggattggat aatatcatcc aatggtcttt
    11281 accagtattg atgcttttgt atccattgtc acttgcgtta atcttacttt ccttaacagc
    11341 aaaattcttc caaaagagtc catttgtcta tcaagtaacg atgttctttt ctgccgttcc
    11401 agccgtattg gatatgttgg caaattcgcc tgcattagtg agtcagcaaa ctgtcgtagc
    11461 aagcttacta ggattttatc atcattatgt tccttttgct gcattaggtc ttggctgggt
    11521 cgtcccaaca ttgcttggtt acgctggcag cttgctcttt tattatgtat atcgcgtatc
    11581 aggatataaa caagaagcaa gtgagttacc agaagaataa ccaaatcaaa cacaatttaa
    11641 cataagagat attaaacagg aaaaaaacag ctagaagaaa
//
LOCUS       DAZUMV010000085        11479 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000085 DAZUMV010000000
VERSION     DAZUMV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.085"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(175..660)
                     /locus_tag="ACG8FK_001948"
     CDS             complement(175..660)
                     /locus_tag="ACG8FK_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS5097544.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    750..1095
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1140..1781
                     /locus_tag="ACG8FK_001949"
     CDS             1140..1781
                     /locus_tag="ACG8FK_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5097545.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1800..2435
                     /locus_tag="ACG8FK_001950"
     CDS             1800..2435
                     /locus_tag="ACG8FK_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5097546.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2453..3268
                     /locus_tag="ACG8FK_001951"
     CDS             2453..3268
                     /locus_tag="ACG8FK_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5097547.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3587..5023
                     /locus_tag="ACG8FK_001952"
     CDS             3587..5023
                     /locus_tag="ACG8FK_001952"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5097548.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5212..7659
                     /locus_tag="ACG8FK_001953"
     CDS             5212..7659
                     /locus_tag="ACG8FK_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS5097549.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVILLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7889..8956
                     /gene="rlmN"
                     /locus_tag="ACG8FK_001954"
     CDS             7889..8956
                     /gene="rlmN"
                     /locus_tag="ACG8FK_001954"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS5097550.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8946..9152
                     /locus_tag="ACG8FK_001955"
     CDS             8946..9152
                     /locus_tag="ACG8FK_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097551.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9149..10252)
                     /locus_tag="ACG8FK_001956"
     CDS             complement(9149..10252)
                     /locus_tag="ACG8FK_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5097552.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10805..11353
                     /gene="aac(6')"
                     /locus_tag="ACG8FK_001957"
     CDS             10805..11353
                     /gene="aac(6')"
                     /locus_tag="ACG8FK_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5097553.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3810 a         1941 c         2480 g         3248 t
ORIGIN      
        1 tttcgtagaa tcaataaata tggatttggc taactatgga agaatactcg tattagtttt
       61 cagcgttcat actagttgtt acattgtttg attggccaaa agaatttctg atatattttg
      121 tgctaaataa agaaaaagac gcttgaattg ggaaataaga aaactactct atcattacaa
      181 attttcataa actggcagat gttctaagta aacaagtatc ttttcaaagt cggaaaaatc
      241 cataatcaac gtagtggtat tgatatttgg atgaaagcct acggtatctt gacgatcaat
      301 agcagcatcg atcacgactt ggattttttt ctctttgtcg tgcatcaatg ctaatggagt
      361 cactgtacct gctggaacgc ctaggaggtc aggaagctgt tcttcagaaa caaaagataa
      421 gcgcttttct tttaattgtt cagctagttg ttttaaatcg gcgtgctttt catcaggcaa
      481 gatgacaaga tagatttgtt ttccttttct tgatttcaat aatagatttt ttacttgcgg
      541 accaggtagg gaaaaaggga catttttgac tgacgtaatc gcaggatgat cgactttttg
      601 aaaagagata cccagttttt ttagtagttc ataagcttct tgttcggttg cacgactcat
      661 cgcgcatcac cctccttttt ttattagtgt atcataaaaa agagttgaca aatgaaaaat
      721 aatcatgtaa attacacgta aagttaaaac aaatgacatc gaaaagaaga gtagccgtta
      781 ggtgttttta gagagtctct ggttggtgga aagaggcagc agttaaccgt gaaacacatc
      841 ttggagtcga ttttctgaag cgtatagtag gaaaatccgg ggagcccgtt acagctgcgg
      901 tcgttgctag atgaccagat aaggctggct tttccgtgag gagtcggtaa aaaaaggtgg
      961 aaccacgtga ttaacgtcct ttgatcttcg gatcaaagga ctttttgttt tcatctgaac
     1021 ggcaacgact gtgtgaggtt ttttctgcaa ggaaaaaata aacagaggtg gaaccacgta
     1081 taatacgtcc tcttggcatt tgccaagagg atttttttat tttaaaggag gaaaagaaga
     1141 tggagtattt gattgaagtt atgccgcaat tattagtagg agcactcaca acattgaaat
     1201 tatttttatt taccttgatt ggctcattac cattagggat tgttttagca ttaggattgt
     1261 ctttgcattt taagccgcta cgctacttgc ttcaaacata tgtatgggtt atgcggggaa
     1321 caccgctgtt gttgcaactg atttttattt tttatgggtt gccgacgatt gggatcgttt
     1381 ttgatcgttt cgaagcagca ctcattgcct ttatcttaaa ctatgcagct tattttgcag
     1441 agatctttcg tggagggata caatcgattc ctcaaggtca atatgaagca gcgcaagttt
     1501 tgcaattaag tccctttcaa acggtgacga aaatcgtcat cccacaagtg acaaagattg
     1561 ttttaccttc tgtagggaat gaagttatca acttggtcaa agattcttcc ttgatctata
     1621 tattaggttt aggtgacgta atgcgtgctg ggaaaatcgc gatggaacgc gacgcgactt
     1681 tactgccttt gctaggcgtg gcaatcatct atcttacatt gactggtatt ctaacaatcg
     1741 taatgaaaca agttgaagga aaattaaatt attaccgcta actgatagga ggaagaaata
     1801 tgattgaatt gaagaaagtg aacaaagcat ttggtgaaaa aaaaataatc aaagatttga
     1861 atctaatcat tccagatggg aaaacattag ctatcgtagg tccttccggt ggcggtaaaa
     1921 ccacgctttt acgtatcttg gctggcttag aaagttctga cagcggagaa ttttatttag
     1981 acggtgtacc gttcaaccca ttcgagctta aaatgaaaga acaagttgta ggagtcgtat
     2041 tccaagactt ccagctattt ccacatctga atatttttga aaacattacc ttagcaccaa
     2101 aaatggtatt gaaacagaca aaagaagaat acaccaaaaa agcagaagaa ttagcggctt
     2161 cattagggat tagtgattta ttgaatatct atccttatca gctttctggc ggacaaaaac
     2221 agcgggtagc gattgccaga gcattggcta tgaatccacg ggtattggct tatgatgaac
     2281 caacaagtgc tttagatcct gaattacgcc agcaagtcga gcgtttgatc ctatcattga
     2341 aagaagatgg ggtaacccaa attattgtca cccatgattt agtatttgca gaaaatgtcg
     2401 gtgatcaatt actaaaagtt tccccaattc attaagaaag gagaagatag agatgaaaaa
     2461 aaccagtttt ctattcgctt tgatcgcagg tcttcttctt tttgcaggct gttcgaacaa
     2521 aaaaacttca gcagataatt gggcgactat caacgaagag aaaaaaatcg ttataggttt
     2581 agacgataca tttgttccta tgggatttag agataaagac gggaagctca caggatttga
     2641 tattgattta gccaaagcag tctttgaaca atatgggatt acggtagact ttcagcccat
     2701 tgactggtca atgaaggaat ttgaactgaa caatggaacg atcgatttga tatggaacgg
     2761 ctattccaaa acgccggcaa gagaaaagaa agtgcaattt acaaaacctt atatggaaaa
     2821 cgatcaagtg ctgatcacac ctaaagaatc aggaatcaca aactttggac aaatgaataa
     2881 taagagatta ggtgcgcaaa atgggtcatc tggttatgat gtatttacga aacaaccaga
     2941 agtattaaaa gagatagtca aaaaccaaga tgctgttttg tatgatagtt ttaatgaagc
     3001 attgatcgat ctgaaaagca gacgggtcga tggtctgctg atggataaag tatacgcaga
     3061 ctattatttg aaacaaagaa acgaactaga agacttcaac ataacaaaag gttcttatga
     3121 gagtgaggat tttgcagttg gtgcacgcaa gtcagatagt acattagtga aaaaaatcaa
     3181 tgaagcccta gatcaactag agaaaacagg tgaattccaa aaaatatccg ataaatggtt
     3241 tggcgaagat gttacgccga gaaattaaca aataggggac tgtgacaaaa gacgtgtcac
     3301 agtccctttt tccgaataaa cagtgttctc ctgccaactc cttgtgactc ggagctgaat
     3361 ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc agtttttcgg tattaagcca
     3421 aatttatgca aaaatgtaga aagccatttt cacaaaattt ttcttactac tcgaaactaa
     3481 gcgacttttt cacaacctcc tttacataat tttattttgt tgtggaaggg gtttcgtgtt
     3541 attctaaaag aggaacgatt gaaggttata ggaaaggaag agatgaatgg aacacaaaga
     3601 acttacctca tttccaaaag attttctgtg gggctctgct tctgcagcgt atcaagtaga
     3661 aggagcttgg caagaagatg ggaaaggaga atcagtctgg gatcgatttg tacggatacc
     3721 tggaaagaca tttaaaggaa cgaacggtga tttggcagta gaccactatc atcgttataa
     3781 agaagatatt gctttaatga aagaacaagg tctgaaagct taccgcttct ctgtagcttg
     3841 gacacggatt tttcctaatg gacgaggaga agtcaatcaa gctgggctcg ctttttatga
     3901 gagattgatc gacgaattga ttgaaaacga tattgaacct atgttgactt tatatcattg
     3961 ggatcttcca caagcattgc aagatgaata taatggttgg gaatcacgtc agatcattga
     4021 agatttcact cattatgcag aaacattatt tgaggcattc cgcgggaaag tccattactg
     4081 gattagtttg aatgaacaaa atatcttcac ttcattaggt tatttgttag cagcacatcc
     4141 gccaggtgtg acagacccga aacgaatgta tgaagttaac catattgcta atttagcaaa
     4201 tgcatctgtc atcaataaat ttcacgagtt agaaatgcct ggaaaaatcg gaccaagctt
     4261 tgcgtatacg ccaaattatc caattgacag taatcctgaa aatgttctgg ctgctgaaaa
     4321 tgcagaagac ctgatggcca attattggat ggatgtctat atgtggggaa aatatccgat
     4381 tgctgccatg aggttcttag aagaaaaagg atgggcacca acgattgaag ctggcgatgc
     4441 agaattgcta gaatctgcta aaccagattt tcttgggatc aattactatc aaacggcgac
     4501 gaatgcattc aatccattaa acggagttgg tgctggaaaa atgaatacga caggaaaaaa
     4561 aggttcatca gaagaaactg gagttcctgg aatgtataaa aaagttgaga atccatttgt
     4621 cgaacgaaca aattgggact gggagattga cccagaagga ttaaggatcg gactgcgtcg
     4681 tatcacaagc cgttaccgca tgccggtact gattacggaa aatggcttag gagagtatga
     4741 caagttgaca gaagataaac aaatccatga cgactatcga atcaattact tacaaagcca
     4801 cgtcaaagcc atcaaagaag caatttcgga tggagcagaa gtcctaggct attgcacctg
     4861 gtcttataca gatttgctta gctggctgaa cggttatcaa aagcgatatg gatttgtata
     4921 tgttgatcaa gacgaaacac aagaaggatc tttggaacga tataagaaag acagttatta
     4981 ctggtaccaa aaactgattg aagaaaatgg agagaacctt tagttcacat acttaaaaca
     5041 agaaggtacg gctggatatg gccgtgcctt ttcttgataa aatcatttag aaattaagaa
     5101 aaactgtaaa ataagctgaa aaatgaaaga aagcgtcagt tctttcaaac aaaactttag
     5161 ttcccttact tttttttgta taatgaaact tgatgaaatg agggataata aatggcacaa
     5221 aagaaacggc ctactaaaaa aaggaaaaag acgaaaaaaa cgaaaaagca aaaacagcag
     5281 caggaacaaa tgctgacgat tggtgttggg tttgccttga ttttatttgc tgtcttcggc
     5341 tttttaaaat taggtttttt aggaatattg attgcaaacg gctttcggat cattgcagga
     5401 aatacttacc aaatcctctg tcttttatta gccgtattag gattttggat tgtcattaag
     5461 aatacagagt tttcaattgg taaaaaccga cgctggtttg gcggcatact cttttatttc
     5521 ggtatcttat tattgttaca tgcccattta tttggcaaac tccatactgg ggaaccaaat
     5581 atcatgggga cgacatggga tttgttagcc agtgatataa aacaaagtca agtagacaat
     5641 aacgttggtg gtgggatgat tggagcaatc ctttatcatt ttacttactt cttgattgca
     5701 cagcctggaa gttatttagc agcagtgata ttactggcag gaggagcatt cctgttcagt
     5761 aatctggaag gctatcagct gttaaatggt atccaatcaa ttggagaacg agtacaggaa
     5821 ttattggaag gtgatcctgc caagcaagct cgaaagcaag cggcaaagga agagagaatg
     5881 aaacagcgcg cagaagcgaa agaagcaagg cgcttagctg caaaagaagc tgcagaaaaa
     5941 gaagctgcag aatatgagaa gaaaaaagcg gtgagccagc aaagggaaaa tactgctgat
     6001 gaatggcagg aaccagaaaa tacagagccg gagcagctaa gttttgttcc aatcgacagt
     6061 tttcaagaaa atatccatcc tgcgaatcta gagaaaccag taccagatac accaaaacag
     6121 accaatacag cagaaggttt ttcagatgag ttgccagagg atgacggtac ctcattagaa
     6181 tttgagattg aagcagaaca ggaaaatcaa gactatgaat taccgacagt tgatttgctt
     6241 gattctatac caactgtgga tcaaagtgat gagtataaaa aaattgaaaa aaatatcggc
     6301 gtattagaac aaactttcca aagtttcggt gtcgatgcca aggtcgtcaa agccagctta
     6361 ggaccatccg tcacaaaatt cgaagtccag ccggctgtag gggtaaaagt aagtaaaatt
     6421 gtgaacttaa cagatgatat cgcgcttgct ttagcggcga aagatgtccg gatggaagca
     6481 ccgattccag gtaaatcact gattggtatt gaagtaccaa atggaaagat cagtatggtt
     6541 tcctttagag aaatcatcga agcacaacca aaccacccag ataaattact agaagtacct
     6601 ttaggacgtg atgtttctgg acgggtacaa acggctgatc tttcgaagat gcctcactta
     6661 ctcgtggctg gttcgactgg tagtgggaag tcagtagcaa ttaatgggat tatcacaagt
     6721 atattaatgc gtgctaagcc ccatgaagta aaactgatga tgatcgatcc gaaaatggtt
     6781 gaattgaata tgtacaacgg catacctcat ctgttgacgc cggtcgtgac gaaccctaga
     6841 aaagcagcgc aagccttaca aaaagtagta caagaaatgg aagaacgata tgaaaaattc
     6901 gcggcaaccg gagtaagaaa tatttctggt tacaatgaat ttgttcaaca gaaaaacttg
     6961 gaaaacggaa cgaaacatcc aaccttacca ttcatcgtag tgatcgtaga tgaattagct
     7021 gatttgatga tggtagccag caatgaagtt gaagatgcga ttattcgttt agcgcaaatg
     7081 gctcgggcag cggggattca tatgatccta gctactcaac gtccaagtgt ggatgttatc
     7141 acaggaatca tcaaagcaaa tgtaccttcc agaatggcat ttgcggtatc aagcggtaca
     7201 gattctcgga cgatcattga ttctaatggg gcagaaaagc tcttagggcg aggagatatg
     7261 ctattcttgc caatgggtga gaataaaccg atccgtgtgc aaggagcatt catttctgac
     7321 cacgaagtag aacgggtcgt tcaatttgtt acagatcagc aagaagctca ttatgaagag
     7381 aaaatgatgc cgacagatga agttgaaacg acaggtgctc cagaacagcc acaagatgag
     7441 ctgtttgaag aagcaaaagc acttgtcgtt gaaatgcaaa cggcaagtat ttcccttctc
     7501 caaagaagat ttcggatcgg ttacaaccga gcagcacgtt tagtagatga actggaagct
     7561 catggagttg ttggcccgtc agaaggaagc aagcctcgga aagtattcat tgagcaggaa
     7621 gaaacatcag aaacgttgcc ggacgataat gacatgtaaa acttgtgtga tgaagggttg
     7681 aaaaacgaat aaccaataaa aagacaaggc atgatgatac caaaagaaaa aatggttttt
     7741 ttccatcttt tctaatacgg tatgatgtct tgtcttttta gtttttgtga gattaaagtt
     7801 ctattcgatt tatttgtgaa gtattgcatt aaggaaatga atcatgtata gtatgtagcg
     7861 aaactttgag aaaaagaggg aaaatacttt ggaaaaacag tccatttatg gcttgacgaa
     7921 tgaagaatta atcaattggt ttatagaaaa cggcgagaaa aaattccgag ctgcccaagt
     7981 ttgggaatgg ttgtatcaaa aacgtgtaag taatttcaca gaaatgacga atctatcgaa
     8041 acaattgatc gaaaaattat cggagcattt tatcatcaat cctctaaaac agatggtcgt
     8101 ccaagaagct tcggatggta cagtaaaata tttgtttgaa ctccctgata aaaacatgat
     8161 tgagactgta ctgatgagac aagaatatgg cttgtcagtt tgtgtgacga cacaagtagg
     8221 atgtaatatc ggctgtacgt tttgtgcaag cgggctgctc aaaaagaacc gtgatctgac
     8281 tgctggagaa attgttgcac agatcatgat ggtccaacat tattttgatg agcgcaatct
     8341 tggcgaacgc gtttcacatg tcgttgtaat ggggatcggt gagccatttg ataattacga
     8401 caatgtaatg gactttttgc atatcatcaa tgatgccaaa ggattggcaa ttggtgctcg
     8461 gcacattaca gtttccacta gtggattagc acacaaaatc aaggaattcg ctaataacgg
     8521 cttgcaagtc aatctggcta tttccttgca cgcgccgaat aatgaggtgc gaacatcgat
     8581 catgcggatc aaccgaagtt tcccaattga gaaattgatg gaagctgtgg atgagtattt
     8641 agagaaaaca aaccgtcgaa tcacgtttga atatatcatg ttgaatcaag taaatgatag
     8701 accagagcat gcgcaacaat tagcagatct attaaaagat aagaaaaaat tagcatatgt
     8761 caacttgatt ccatataatc cagtcagtga acatgatcaa tattctcgca gtccaaaagc
     8821 tgatgtattg agattttatg atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa
     8881 agaacatggt acagatattg atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa
     8941 aacagttgcc aaataatcgt atcctactcg ttttttcaat taccataaaa ggagcagttt
     9001 tcttaattaa ggaactgttc aacttaaata agagaaaaga ttctatagcg aaagctataa
     9061 agcttttttt gacaagaaaa accctcatca agcaaagaaa tgtcctcaat ttgatgacag
     9121 cctgcttgat gggggtttta gcaattattt aactgatttt cttatatcct tgctggcttt
     9181 cttgtgtagc atgataagtt tctacgtcaa gaatggcctg tttaaatttt tctcgggtga
     9241 tttcataagg cacatgttta atatcatttg ttgccatggc tttatctaag attgcttcta
     9301 aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac tggaagttgg ttctttttca
     9361 taaacacata gattttatca aacaaatcga actgctgatc catcatcaat aaggtcagta
     9421 caccataact gacagatgct ccgtgaaggt atccctccat tttttctaga acagtcgtac
     9481 cgtaatagaa tgagtgtgcc atagcagaat tatagttatt gttaactaaa acagaaacca
     9541 atccggtagt aacaatgata tccgaaacga cttgttcaat tgcatatgag ggacgatcgt
     9601 cggcactatc tttcaatgct tgcgtaccat aagtcaagag acgatcaatg cacatttttc
     9661 cgatttggat tcctaatcta tccgtgtagc ctagatcatc gccacgagca gaaaaagtag
     9721 attcgacttc tttggataaa gcatcgccaa tacctgccca taagtattta tcaggtgctt
     9781 cggcaataat tgctgtatca atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc
     9841 cgtccattac gccttcttcg ttatagatga cacataccgc agtgacgggc gaacagttcg
     9901 aaccaatagt gggaaaggtg aagactggtt tagctagttt ttcaccaatg acttttacag
     9961 tatcgcaagc acggccacca ccgactgcaa acaggacgtc tgctttttgg acactttcat
    10021 gctggatcaa ctgttcaata tttccataag ttgaattacc cccgtaccag agactggcga
    10081 caacctctat ttcctgtgtc aaagctactt ttattttgtt ttctgccttt gaaagtgcag
    10141 tcttccctcc aatcagcact acttttgtcc catattcttt gacgattcca tttgcttttt
    10201 catacgcatc tgttccaatg gtataatttg gtaagaaaac actttgactc atattcattt
    10261 ccttctttct tcgttgaaac gggttaaaat tttaaagagt gatcgatctg actgcggctg
    10321 tagatggagt agaggtgaac ctaagacagg ataagtgatg aataaggtgg catgaacacc
    10381 ttgaaatcat caataagtat cctaaaaacg aacaagtaca aaaacattga aaacgacaat
    10441 aatgaatttt tgttcctctt agtatttcta ccgattccct ttgctttttt gtaattatct
    10501 aaaaattcta aaaattaaga ttattatatg aaaacaggtt ttatttgtca attcataatt
    10561 ggtttttttt aaagcaaaag ataatctata tcatgatgaa caaacagagt tttttcctcc
    10621 aattgatggc tttcctgatc tttcctattt tgaataaagt gtttctgtat aattttttta
    10681 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
    10741 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
    10801 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
    10861 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
    10921 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
    10981 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
    11041 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
    11101 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
    11161 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
    11221 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
    11281 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
    11341 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
    11401 aatttacgaa aattacttct tgaatttttg tgaaattcca acttatttcc gaagaagcta
    11461 cttttggaac accgtgtac
//
LOCUS       DAZUMV010000086        11322 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000086 DAZUMV010000000
VERSION     DAZUMV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.086"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8FK_001958"
     CDS             complement(<1..132)
                     /locus_tag="ACG8FK_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5097554.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            541..2913
                     /locus_tag="ACG8FK_001959"
     CDS             541..2913
                     /locus_tag="ACG8FK_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS5097555.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2974..4434
                     /locus_tag="ACG8FK_001960"
     CDS             2974..4434
                     /locus_tag="ACG8FK_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS5097556.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            4445..6649
                     /gene="topB"
                     /locus_tag="ACG8FK_001961"
     CDS             4445..6649
                     /gene="topB"
                     /locus_tag="ACG8FK_001961"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5097557.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6665..6814
                     /locus_tag="ACG8FK_001962"
     CDS             6665..6814
                     /locus_tag="ACG8FK_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097558.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6889..7482
                     /locus_tag="ACG8FK_001963"
     CDS             6889..7482
                     /locus_tag="ACG8FK_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS5097559.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            7495..8394
                     /locus_tag="ACG8FK_001964"
     CDS             7495..8394
                     /locus_tag="ACG8FK_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS5097560.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            8397..8894
                     /gene="ssb"
                     /locus_tag="ACG8FK_001965"
     CDS             8397..8894
                     /gene="ssb"
                     /locus_tag="ACG8FK_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5097561.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            9260..9520
                     /locus_tag="ACG8FK_001966"
     CDS             9260..9520
                     /locus_tag="ACG8FK_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097562.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9965..10171
                     /locus_tag="ACG8FK_001967"
     CDS             9965..10171
                     /locus_tag="ACG8FK_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS5097563.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            10192..10422
                     /locus_tag="ACG8FK_001968"
     CDS             10192..10422
                     /locus_tag="ACG8FK_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097564.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            10438..10875
                     /locus_tag="ACG8FK_001969"
     CDS             10438..10875
                     /locus_tag="ACG8FK_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097565.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10868..>11322
                     /locus_tag="ACG8FK_001970"
     CDS             10868..>11322
                     /locus_tag="ACG8FK_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097566.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDY"
BASE COUNT         4273 a         1895 c         2359 g         2795 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaagaac
      541 atggcagaag aaaaagaacc actagacaat agtcgtttgg gaaaatccaa aagaaaacta
      601 gtacgattgc aaaatgaatt aaacgaacaa attgaaaaga tgtttgaaca ccaaaaaatg
      661 acgaacggtc aaccaatgaa cgacaagagg aatggccgtt catggtttaa ccagcaagaa
      721 cgaatcgaaa ataaagtcca aagtctgaga gaagagatca agcaacaaga agaatacgtg
      781 gaaaagcttg aaagacaaga agagctaaag gaaatgggct ataacaagta cggcggactg
      841 gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
      901 gaatcggctt tttcagccgc cacgatccga aaataccaac ggaagttgga aacattagaa
      961 cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt acaagcgatc
     1021 attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt tttgaaaggc
     1081 tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc aaaaaaatac
     1141 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctagacca
     1201 actgattcta tgaaggagaa agaaaacgat catttctatc gagtagaatt caacgaaaat
     1261 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattcga attgatcgat
     1321 caactcaaaa cattggataa ccaatacgcc gcagaagcag gttattataa attctatttt
     1381 gaggaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
     1441 aacagtaatc aaccaatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
     1501 gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa
     1561 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1621 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc tcctgaagac
     1681 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1741 ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
     1801 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1861 aaagggtttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac accagaagat
     1921 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaccttacta tacgattgaa
     1981 aaagtgtttg atatcacaca aacacagtta aaacctgaag actatccgaa aatctttccg
     2041 aaccgtgtct ttgattttca attggataaa aaaggacagt cagaacttca agctgggatt
     2101 caagcggtag ccaacaatat tggaatacaa attcgagata tgacagaaag tgaagtctat
     2161 caaagagaat taggacaagc acgtggggcg tatgttcgta acgaatttac tggcgaagaa
     2221 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     2281 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     2341 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtc ggagaaagct
     2401 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     2461 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     2521 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     2581 caggagcata aaaatacttt agaaacgcca gagaataaga atattgtgcc cgttggttca
     2641 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     2701 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtaatttt aaaaggaaaa
     2761 agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct aggaaatcca
     2821 ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga aatctattat
     2881 gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact atctgaaaaa
     2941 cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat gagtcttatt
     3001 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
     3061 aataaaggac gagattatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     3121 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     3181 tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc cactgatttt
     3241 attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga aacagaaaag
     3301 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     3361 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgcc
     3421 gaactaaagc caaaatatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
     3481 gcggtcgcct ttttatggaa agatccacag acaaaagaga cagtaggggc gagttatcaa
     3541 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
     3601 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gataaaattt
     3661 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
     3721 acgtggttag tctctatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
     3781 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
     3841 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agtcgatcca
     3901 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
     3961 gaatacaaaa tcatttatga agaagtcgca aaagaagaaa aggtcacacc agaagctatt
     4021 atggccatac acaagacaga aaacaatctg caattaacga accaactggt ttccgctcac
     4081 aaagtaaatg cgttttttgg ccagcagctt tctgttaacg attcaataga agccattaat
     4141 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag tttgtgaacg cgtggatggt
     4201 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgcgca gattcttttc
     4261 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
     4321 gaagtaaaaa aggactggaa cgatcaattg aagcatgaga tccagcaaca agaaatcaga
     4381 aagcaaaaac gtgccatgtt gtttcaacaa ggcagacaac aagaaaggga gtgaaatcgt
     4441 catgatgaaa accgttattt tagcagaaaa gccgtcacaa gcgaaagctt atgccgatag
     4501 cttctctaaa gccactcgaa aagacgggta ttttgaaatc caagatcgat tgtttcctgg
     4561 tgaaactgtg atcacgtatg gttttggtca tttagtcgaa ttagattcgc ccgatatgta
     4621 cgatgaaaat tggaaacaat ggtcattaga acatttgcct attttcccta cacattatca
     4681 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     4741 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tcgcttggac
     4801 gatcattcaa caagtaggcg ccgatcaagg aaaaaccttt aaaagacttt ggatcaactc
     4861 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagacgcaa aagagaccta
     4921 tccaaaattt gaagaagcac aagcacgtca gatcgccgat tggttaattg ggatgaacgg
     4981 tagcccactg tatagtttac tcttgcagca aaaaggcata ccaggaagct tttccttagg
     5041 cagagtccaa accccaacgc tttacatgat ttatcaattg caggaaaaga ttagaaattt
     5101 caagaaagaa ccttattttg aaggaaaatc cgaagtaatc gctcaaaatg gcgcatttga
     5161 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     5221 agaaaagggg gttcaattgg gaaaacagcc aggcatgatt cgccaagttg aaaccgagaa
     5281 aaaaagcgcg gcaagtccac gtcttttttc tctatccagc cttcagtcta aaatgaatca
     5341 gctcatgaaa gctagtgcca aagacacttt ggaagccatg caaggcttgt atgaagggaa
     5401 atatctaagc tatccgcgaa cggatacccc ttacattacg gaaggggaat atgcgtatct
     5461 gttggatcac ttagacgaat acaagcattt tttgagggct gaagaaattt cgactcctat
     5521 tcatacaccg aatagtaggt acgtgaacaa caagaaagtc caggaacatt acgcaatcat
     5581 tcctacaaaa acagttatga cagaagcgac gtatgaaagt ctgacacctt tacaacaagc
     5641 gatttacgag caagttttaa aaacgacagt ggcgatgttt gcggaaaaat atacctatga
     5701 agaaacaacg atcttgacgc aagtccagca gcttcagtta aaggcaatcg gaaaagtacc
     5761 gatagatcta ggttggaaga agttatttgg caaagagagc gacggaaaag agaaggaaga
     5821 agagccgcta cttcctaaag tgacaaaagg agaaatggtg acggtagatc tacaagtcct
     5881 tgaaaaagaa accaagccac cacaacctta taccgaaggt acgttgatca ctgcgatgaa
     5941 aacagctggg aaaaccgtgg atagtgaaga agcgcaatcc atattgaaag aagtggaagg
     6001 aatcggtaca gaagcgacac gggcgaatat tattgaaacc ttgaaacaaa aggaatatat
     6061 caaggtagag aaaaataagc ttgtggtgac gaataaaggg gttttgcttt gccaagctgt
     6121 ggaaaaagag ccgctgctta cgagtgccga aatgaccgcc aaatgggaaa gctacttgtt
     6181 aaagattgga gaacgaaaag gaacacaaac cacttttcta gccaatatcc aaaagtttgt
     6241 ctctcactta ttggaagtcg tcccaggaca aatccaatcc attgattttg gatcgacgtt
     6301 acaagaagtg aaagcagcca gtgaaaagca agaagccgcg cgtcatttag gaatttgtcc
     6361 aaaatgccag gaacaagaag tcttactcta tcaaaaggtg gcagcttgta cgtctgaagc
     6421 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcga cacagctgaa
     6481 ggagattatt caaaatagcc gaacaagcca accagtgaaa gggttaaaag gtcagaaggg
     6541 ttcgtttgaa gcaacgattg ttttaaaaga agacttcact acaggctttg aattttctga
     6601 aaagaaaaaa acgaacttca agaaaagaac acgacgcacg acaaaataat ggagggttaa
     6661 tcttatgcgt aatacaacac acaaacaaca agaaatcgaa aatcaaaaag ccaaaattcg
     6721 ttcagaacaa gagaaattac gtgccagtaa aagaagactt cagcaattaa agaaccagca
     6781 acggattcaa aaagaaatcg aattagatcg atagacatta accttgttac aaatacacta
     6841 tcatgatata tttaatgtag tgtatttgta acaaaagggg ggaagtaaat gcaagagaaa
     6901 ctgaaagaat tgttggctat tcgcgatgga aatttatcta tgaaggaagc cagaaaatta
     6961 ggaatcgcag caaccactgt acaaagattg gtgaatcaag gaaaattgat caaagttgat
     7021 cgtggttatt atgtagagga tggtcatgga atagatgatt tattttggct tcagcaacgt
     7081 ttttccaggg gaatcttctc tcatgaaacg actttagata tttaccagct ttctacgaat
     7141 atgccgaaat tcattcactt aacttttccc catggatata acgttgatcg ttcaattaca
     7201 aaagagcagc agttacaatt tcatttcgtg aagaaagaag tttatgaact aggaaaagta
     7261 gagggaactt cctttcaagg aaatcctatt actatgtatg ataaagagcg tacactttgt
     7321 gatatttgga atccgagata tgaaattgaa tatgaaatga aattagaagc attaaaagag
     7381 tacatggaag acccattgag agatccaagt aaattaagag attacatgac aaaactacat
     7441 gtagaaaaag aaatgaaata ccacatgcaa agcttatatt gatggaggaa aaagatgaca
     7501 ccgacacaat ttgcagcaaa aacaagaaat ttagcaaaga ggacaggatt aaatgcacag
     7561 ttagtacaac ggcattttat gatggatcgt ttaattgaac aaatttctga atctaagtat
     7621 aaaaatgatt tcattttgaa aggtggcttt ttattaggat caaaatatgg aatcgataga
     7681 aggacgacaa ttgatatcga cacgacattt agaaatagca aattaactga agaaaaattg
     7741 aagagtattt ttaatgattt aactaggaaa cccacgaaag aaggtattgt tttctctatt
     7801 caaggaatga aggaaacaag agaagccgat ttttatcctg gatttcaaat taaattggtg
     7861 gctaatttag agaaagtacg tgtgccattt aaaatggacg tgacaacagg agattctatt
     7921 tatcctgaag tagatacaca ctatcaccaa ttaatgtttg aagataaaaa ggtggagatt
     7981 ccagcttatc caacagcaca aattattgca gaaaagctaa gtgctacgtt cagctttggc
     8041 acagataatt cgagagcaaa agattactac gacttattta ccattccgaa actagaaaat
     8101 atcgatacga aagagttata caaaagtgtt cgaaatacat ttacaaaacg tggggataca
     8161 aaaccgttgt ccttttatta tgaaagagaa atggataaga tcataggaaa tgactatttg
     8221 gaaaagcagt gggaaaaata tcgctcagtt aacacgtttg cgaattccat ttcatttaca
     8281 gatacagtca acgaaattga tcacctaatg aaaagtgtga tcaaaattga aaatcaagaa
     8341 agaaaacggc agtttaaaag atcacaacaa atgaatcaag aaatggagag ataaagatga
     8401 tcaataatgt aactttagta ggacgcttaa ccaaagatcc agatttacgt tataccgcga
     8461 gtggtactgc agtagcgact tttaccctag ctgtcaaccg aaatttcacg aatcaaaacg
     8521 gcaatcgaga agctgatttt attaactgtg tgatttggcg caaaccagca gaaacaatgg
     8581 tgacattagc taaaaaaggt agcttgattg gtgtagttgg gcggattcaa acgcggactt
     8641 atgacaacca gcaaggacaa cgtgtgtatg tgacagaagt tgtggccgat aatttccaac
     8701 tattagaaag caaagcagcg acagaaagcc gtgcgcacgc tgatcaatcg tccaccagtc
     8761 caagcaccac cacttttgaa caacgggata cagcgacgcc taataacaat ggattgaacg
     8821 ctttacagaa tccatttggg aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8881 atctaccgtt ctaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8941 tagtcaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggt
     9001 tttcttttac gtttctatta taagaattaa cgggtaggct tactgtcttt cttggcaaat
     9061 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     9121 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     9181 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     9241 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     9301 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     9361 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     9421 tattgccgtc acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt
     9481 tagaaatccc gatcatttta cgcgatcgat gggcatataa aaaaagaaga gttgcaatcc
     9541 taatgtagga ctgcagctct ttttgtttta catagaaaca agaaaaatga aaaggccttt
     9601 ttaggaaggc gcgagcgcct tctcaattga aaaacctctc tcactccaac cgccaccgcc
     9661 gttcgagaag aaaggaagtg aacgttgcac gttcactaag ataaagggtc ttgcaaagca
     9721 agacaaaata gaaaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta
     9781 ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg
     9841 taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgattcatt ctggccaaca
     9901 aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc
     9961 aatcatgcga aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta
    10021 tcatgcacca catactgtcg aatgcgattg tggacaaaga gcgaaatgta tcaaaagcta
    10081 tcgtttgtat caatgtccaa catgcggaaa aaaatacacc ttgtcttatg gcgattatat
    10141 tttgattgat gagaaaggaa agaaacgctg atgaaaacat aacataaatt attgactgta
    10201 aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcagaagct
    10261 ttacttattc cgcggtactg gacgatagaa acccctttag tagaggatgc accgactatg
    10321 ctatttgaag acctagtgaa gattgcagga tatgaactgc attatcaaga aaacatggaa
    10381 acttatgaaa acgaatattg gttaaccaaa ttagagaact aaaaaggaga aaaaaatatg
    10441 gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg
    10501 gaggatatga cattcctata ccatgagaca cgtgttccaa aaaaacatta taaggaaaga
    10561 ttgtcggtag ctgtggaaga aatgattgaa tcaggtgtcg aaatgaatct cattgcgacg
    10621 tactatcgaa cactggaaga actgaaaaaa caaaatgcaa aatggttttt ccaagcctta
    10681 ctatgcttgg aagtaggtgt caaaccaagc actattaaac cttctgaata tcaagcttta
    10741 gaactaacgt atgggaaatt tgtcgaaacg aaaaaagcca agactgtttc aagtgaatgg
    10801 ttagattatt ttgaaaatat caataagtat ggcgcctatt acaccatgaa aaaggaggac
    10861 aacgaaaatg aataaggtga tttatcaact aacaacggaa gatgtgtcac actttttaac
    10921 aaacaaagaa catttgaatg aagtaatcag acggattcaa aaagacttta gttgtatgga
    10981 agaaatgaag tactttattc aaagagtcga agaacaattg tatttggaaa gtgaaacgag
    11041 tcaaacgaag gtcacagtca gcaataatta tttagacgtg tataaacatc gtcctgaaac
    11101 ggtaaaaaaa ggaacggagc ttctacgact agaaacagaa aaatacatca tgaatgttta
    11161 cctagaaagg agtgaactgg tcgaaaactt tgtcagctgc acgatttgca ggaaacacta
    11221 ttactcagaa tcagaatatt attcaatttt tgagagcatg gaattaatgg aagaagaaat
    11281 ggaagagcta aacagcaaaa atgcgacgat agacgattat ta
//
LOCUS       DAZUMV010000087        11261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000087 DAZUMV010000000
VERSION     DAZUMV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.087"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            145..1080
                     /gene="pplA"
                     /locus_tag="ACG8FK_001971"
     CDS             145..1080
                     /gene="pplA"
                     /locus_tag="ACG8FK_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS5097567.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1170..2273
                     /locus_tag="ACG8FK_001972"
     CDS             1170..2273
                     /locus_tag="ACG8FK_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS5097568.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2286..3239
                     /gene="menA"
                     /locus_tag="ACG8FK_001973"
     CDS             2286..3239
                     /gene="menA"
                     /locus_tag="ACG8FK_001973"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS5097569.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4053..5399)
                     /gene="gor"
                     /locus_tag="ACG8FK_001974"
     CDS             complement(4053..5399)
                     /gene="gor"
                     /locus_tag="ACG8FK_001974"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS5097570.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5533..6786)
                     /locus_tag="ACG8FK_001975"
     CDS             complement(5533..6786)
                     /locus_tag="ACG8FK_001975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS5097571.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(6792..7715)
                     /locus_tag="ACG8FK_001976"
     CDS             complement(6792..7715)
                     /locus_tag="ACG8FK_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS5097572.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(7936..10428)
                     /locus_tag="ACG8FK_001977"
     CDS             complement(7936..10428)
                     /locus_tag="ACG8FK_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS5097573.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10435..10902)
                     /locus_tag="ACG8FK_001978"
     CDS             complement(10435..10902)
                     /locus_tag="ACG8FK_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS5097574.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(11038..11111)
                     /locus_tag="ACG8FK_001979"
     tRNA            complement(11038..11111)
                     /locus_tag="ACG8FK_001979"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11075..11077),aa:Arg,seq:acg)
     gene            complement(11169..11242)
                     /locus_tag="ACG8FK_001980"
     tRNA            complement(11169..11242)
                     /locus_tag="ACG8FK_001980"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11206..11208),aa:Pro,seq:tgg)
BASE COUNT         3340 a         2310 c         1976 g         3635 t
ORIGIN      
        1 cacgaaattt gctgaaagaa aacgtttgaa atattacatg ggttatttat caaaaatagt
       61 tgacttatag gctaaaaaga ttagaatgta taatgaatca caaagaaaaa ttgtggacta
      121 gaaatggaat ggtggggtat taaaatgaag aaaaaacgtt ttgtaactgg atttgccgta
      181 ttagcatttt cagctctagt gttaggcgct tgcggcgcag atgacaacgg aagctcaaac
      241 tcttcttctg aatcatctac agcacaatct tcgactgcaa aaagcacaac agaatcatct
      301 gcaaaagttg tagctggcgg agatttgcaa gacggcacat ataaattaga agaaaaaaat
      361 tattcaaatg gttaccgtgc agtctttgaa atggttgtaa aagacggcaa aatcactgaa
      421 tcaaaatatg ataacgtcaa tgaaaacggc gaatcaaaaa caaaagatgc agaatacaac
      481 aaaaacatgg aagcaaaatc tggcacaaac ccagaaaaat acattcctga gttgaatgac
      541 caattcttaa aagcacaaag cgcaagcggt gtggaagtcg taactggagc tactcactca
      601 tctgaaagct tccaaaacta cgcacaacaa ttgatccaag ctgctcaagc tggtaacaca
      661 gatacaatcg aaatcgacaa tggtgctgat ttgaaagacg gcacttacaa gcttgaagaa
      721 aagaactact ctaacggcta ccgtgtgcaa ttcgaaatga cagttgctgg cggcaaagta
      781 actgaatcaa actttgatta tatcgacaaa gacggtaaat caaaacaaga tgatacagaa
      841 tataatgaaa atatgaaagc aaaaagcggt acagaaccaa aaacttacat ccctactctg
      901 aacgacgaat tcgtcaaagc aatgggcgaa gaagatggct cacctgctaa tgtagaagtt
      961 gttacaggtg ctacacacag ctcacattca ttcatcatgt acgcacaaca attagtgaat
     1021 gcagctgaaa aaggcgacac tcaaacaatc gaagtagata atatcgtaac agaaaaataa
     1081 tcacataatc atactaataa aagttggagg gtgggacgat agccagatta ggcagtcgtc
     1141 tcacctttca aaatgaagag aggaaaaaga tgcggaaaaa actactattt tccttaatca
     1201 cactgggcat ctcttttatg gtgctaactg gctgcagcaa ctcagcaaat aaaacaacca
     1261 ctgaaagttc agcagctacc aagatattga aagatccata ttctgatgaa cagttcttgt
     1321 tagggactta cgttcgtatc cgtgtgtatg atgaaggcaa agaatcagca ttgaaacctg
     1381 ccttcgatcg agtaaaagaa cttggtgata aaatcacgat caatcaaaaa ggttctgaga
     1441 tcgatgaagt gaatgagcaa gctgggatca aaccagtaaa agtttctgat gacgtctaca
     1501 cgttagtcaa gcgggcctac gaatatagcc aagattctca aggcggattc gatatggcga
     1561 ttggtgcaat cacacaatta tggcgaatcg gttttgatga cgccagaaaa ccttcccaag
     1621 aagaaatcga tcaggcattg aaattagtgg attatcataa gatcgaacta aatgacaaag
     1681 aaaaaactgt ttatctaaaa gaaaaaggaa tgatcatcga tttaggtgca atcgccaaag
     1741 gttacattac ggacgaagta gtgaaagtct tgaaaaaaca aggagtgacc actgccatcg
     1801 ttgacttggg cggtaatgtc tatgtcttag gccacagccc aagaggcgaa aaccaagact
     1861 ggacagtcgg catccaagat ccgaacatgg ctcgcggatc tgttttaggt tctattaaag
     1921 aacggaacaa aacattagtt acctctggaa tttacgagcg ttatttagaa gtagatggca
     1981 aaaaatacca tcatttgttt gatccaaaaa cgggttatcc gttcgacaat gatatcgcaa
     2041 gtgtcacgat cataacagat aaatcaattg atggtgacgg gttgtctaca gcagtctttt
     2101 caatgggtgt gaaaaaagga ctggaatacg tcgaaagcga attgaataac gggacggaag
     2161 cgatcttcgt gacaaaagac gataaagtat atgtgaccga tccaatcaaa gacaccttca
     2221 aattatcgga ggactcccat tacacgatgg gtgaccgttc tgaactaaaa taaggagtac
     2281 gaaccatgac actaaaaatc tttttagaag tagttgaaat acgaacaaag gtggctagtg
     2341 tatttccttt tgcggtaggg gtattattct ctatcgcttt ttttcatgag atacattggc
     2401 taaacacttt tttatttttt attggaatgc tagtttttga tttagcgact accgctatca
     2461 ataattacat ggattatgaa aaggctcatt ctgaagtata taagtatgaa gaaaatgtca
     2521 ttggccgcag aaatctatct cctgtattga ttcgaaacat gattttagct atgattgcat
     2581 ttgtttttgt tatcggcgtt attctaacga tcaatacggg ttggctttta cttttgatgg
     2641 gatttatctg ttgttttatt ggagtattct atacattcgg accgattcct ttatctcgaa
     2701 tgcctttagg ggaaattttc agtggggtga ctatgggcct tggaattttt gccatggtcg
     2761 tttatgtcaa tacattcggt tcagatgtct atgatctgac agtcaacttt tcatctggac
     2821 aatttgattt ggtaggaaat ctttgggcta tagcagcaat cgtattagct tccttaccct
     2881 tggtgtttac gattgccaat atcatgttgg ctaataattt gcgggatctt gaccgtgata
     2941 ttgaaaatca tcgctataca ctagtttttt atatcggacg tcctgcggga acagtcttat
     3001 tccagctatt gatgtatgct tgctatttcg tgattttaat ggggttgttg agcggtatct
     3061 atgaatggcc gatcttggtc accttcttga ccttgccaat tgtgaagaag catttggatc
     3121 aattcaagca aacgttaccc cagcctatca gtttcagcta ttccatcaaa aatatggtct
     3181 tattcaatgc cagttatgta ttggggctat tactaacaat cattatgcaa gccatttaac
     3241 agcatgaaga ggttgtgaaa aaactgccct gcatcaagta ataagaacag ccaaataaaa
     3301 atagctcctt atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
     3361 ccgtttatca ggaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
     3421 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
     3481 caccgtttat cagaggttgt aaaatcagtg ttttgtcctg agtattagag aaacaaatgg
     3541 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa
     3601 cactatttgt tcggaaaaag tgactaacta tgacacaatt tttttcacag tccctttttt
     3661 cacttgattt gattcgtacc agcgaacttg acgagaactt tcttggtttg gtaactatat
     3721 ggtattcttt taacatcctc gtttgcttct tctaatttat ttatacaaag tcacactttt
     3781 ttaaacatct gttcttcgat acatttctat ttcttttaaa catcgatacc aatattttga
     3841 acttaagagt gcataccaaa tggattcatt tactcttcgt cagttacgaa gataatcaaa
     3901 atctccaact tccctactta tttcgttttg agaattatcg aagcaatctt acataaataa
     3961 ctaaaaaaca aaaaaccaga aaatcagtat actgattttc tggttttaca tggtttgttc
     4021 aataaaaagc tgttggaact gttaatatta aattatcgca tcgttacgaa ttcttccgct
     4081 cctgttggat ggatggctac tgtattgtca aagtcttctt tagttgcacc catcttgata
     4141 gccacggcaa aaccttgcag catctcatcg acaccaacac caatagcatg taagccgacg
     4201 attctttctt cttttccgac acagatcaat ttcatttcgc atttttggcg ataatcattt
     4261 aatgcaaagt acattggggt aaagcgtgaa cgataaactt tcaattcatt ttcgccatat
     4321 ttttccaatg cttcttcttc tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg
     4381 actgtcggaa caagtttata gtcaagataa gaatctttct ttccattaaa taaacgttct
     4441 gataaacgtc taccagctgc gattgcaaca ggagtcagat ctaatttacc gatcacatca
     4501 ccaacagcaa aaatatgttc atccgttgta ttttggtatt tatctacttt gataaaccct
     4561 tttttgtcca attccacatt tgtattttcc agacctaatt tttcggtatt cggtacacgt
     4621 ccaccggcaa aaagtacaca atcaccagtt aaggtttcac cattttcaaa tagcaccgta
     4681 tattcgtcat tgtttttagt aatttcttta gcaatatgat gtgcgtaagt ttttattcca
     4741 gcttcttggt acatttggat caaattgtcc gaaagcattt tatcaaacgt tctaagtggt
     4801 cgttcttttc gatatgccca catgacttca ctacctaaac cacgaaatac acctgaaagt
     4861 tcagccgcaa tgtatccagc acctaaaacg attgctctct taggtaactc tgttaattca
     4921 aaaaatccat ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt acttgcacgg
     4981 cctccagtag cgatcaagat tttaggagca cgataggttt caccgtttac ttcgatcgtt
     5041 tgactatcga taaaagtagc gtaacctttt atagcctcga catgattgct atctaatcct
     5101 ctttgataag caccgtgtag aaaatcaata tatttttctc gtctttctac tagaccggcg
     5161 aaatcaacat tttttaaatc tgcttggata ccgtaacttg aggcatctcg ttcaattgtt
     5221 tccaaaatcg tacttgcttg ccacataact tttttaggta cacaaccgac gttgacacat
     5281 gttccgccca attcattcgc ttcaatcagc aaaacttttg cgccatgcat ccctgctctg
     5341 tttgctgaag caataccgcc agagccgcca ccaattacga tgtaatcata tatttgcatt
     5401 ttgttcctcc ttattgaaga gatcaatcaa aacctttatt taacgatcct ttttctacag
     5461 aaaacaagtg taacactttt cttgaatttt gtatgcgaag atgcttaaaa cagggatata
     5521 tcagcttttt ccttatttac gttttcggat catatctcca ggtttcaccg gctgtgcgac
     5581 tggcatagtt aaaatcatca ttggatttgg tgctcggtca atggattcgc cttcttcatt
     5641 atgcataact tcaacggttt gactgaaatg actgaatcct ggaccataaa attcgatttc
     5701 atctcctaca gaaaacaagt ttctctgacg tatcgtagca atctgtgttg cctcatcata
     5761 agataatact tcaccgacaa atttatatac tgggatttta cgacgttcac cgaataattg
     5821 ttcgttttca gttggtgtat tgtagtaaaa acctgtagct aattctcgtt gtgctacttt
     5881 ccataactcg tcgatccatt cttgcttgca gacatagttt tcaggatctt ccatataact
     5941 gtctacagcc gctttataga catttgacac cgtagataca taatggatcg atttcattct
     6001 tccttcgatc ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat gttcgatcat
     6061 tgacatatcc actgcactca tcgagaattc ttcttccact tcgcctgtat cagttaaact
     6121 gtttcgttct tgaccaaaag gcatatcgaa caatccatac ttccagcgac aagattgaga
     6181 acatccgcca cggtttgcat cccgcatcga catatggttg gacaatgtgc atcgaccaga
     6241 ataagagata cacatggcac catggataaa tgcttcgatt tcggtatccg tatttttacg
     6301 aatctctgcc acttcctcca ttgaaacttc acgagctaaa acgacacgtt ccaacccttc
     6361 ttctttccag aattccaatg tctcaaaatt agttgctgaa gcttgagtgg ataaatggat
     6421 cggcagacct ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac
     6481 tgcagaaata ccgacatcac gcaaactgcg gaaaaattcc cctgctccat cttcatttcc
     6541 ttcatgggtg accatattag cagcaacgta taccttcgca tcatgttctt ttgcaaaagc
     6601 aactccttca gccatttctt cataagtaaa attacctgca cggcttctta aaccatatgc
     6661 attcccgcca atatatactg catctgctcc ataatggatc gcggttttta acttttccag
     6721 tgttcctgct ggcgctagga tttctggtcg tttcaatttt ctttttactg tcatttcttt
     6781 catcatactc cttatttgat agcagaagga tcaatataaa agaatcctgt atctaattca
     6841 cggttttcag gatgcaattt actgatttcg ctagtcattt tttccgcttt ttcttgatcc
     6901 cagctacctt ctaaaatttg tttttttgct tcggcgaata gtcgagtgat gtttacgaat
     6961 ttttcgcctg gagtaaataa tccgtccaat ttccatgtac agtaatgatg ctgataaagt
     7021 tcattcaatt cattcgagag attcagatca ttgctggcaa aaatatgtgt gccatgacta
     7081 tcttcataaa tagaataatg tgtctcttct tttttaggtt ccgagataaa aagacctctt
     7141 tcacgatctt tttgttcgtc ttgtttcgta tagttatagt aattctgtag cagtggacgt
     7201 ttggattgat ggatgcatgt agcaccatac acaagggttt ctacaggaat atcgagtttt
     7261 tcttccatcg ccttcatctc ttcaaaagga acttcacgag caagtactgc tccaactgcg
     7321 ccttttttcg cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc agcatcataa
     7381 atatacggta tacgcaattc aggatttttt ttcatcgtat aaataatacc tgtatcacca
     7441 actgtgatca catctacgtt tatttctttt aaaaaagcca aatattcagg tacatttttc
     7501 attttttcag gatgaatgat cccatttaca gcaatattcg cttttttacc atacttgtgt
     7561 gctaattcta ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca
     7621 aattgctctt caccgaaata tagtgtatct gcacctgcag caagcaactg ttctgcttgt
     7681 tcaatagttt ctactgtggt tatgagttcg atcataattt tctcctccta caacttcata
     7741 atagtagcat agctctaaaa actttccaat acttcttcta tttaaagtcg gatttttgaa
     7801 aattatttca caaaaaatca aaataaagtt cgatatattc tgtccaaata taaaaagaga
     7861 ttgtgacaaa aatcttgtca caacctctta atcaagtctt actattataa aaattcagct
     7921 acacaggcca agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa
     7981 tgtgatctta ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc
     8041 aagcaaagcc tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg
     8101 tgcaccatat tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat
     8161 tttcacttga atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac
     8221 gattgtatgg atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt
     8281 caagaattca ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt
     8341 gtgatcttta gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt
     8401 cgctccgata tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt
     8461 agaatctgtt aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg
     8521 tgctttctcc acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt
     8581 caactgtcca ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt
     8641 actgtatttt tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa
     8701 catgacttct gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa
     8761 catgaatgaa ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc
     8821 acgtgaaact gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc
     8881 cagttctaac aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca
     8941 ctccgataca actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg
     9001 ttttgcttca attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg
     9061 taattgagca gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag
     9121 ttcatcgatt tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg
     9181 gactttcgct gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt
     9241 gatataacga acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc
     9301 atggtgctct tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt
     9361 tggctcatct acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata
     9421 tttttgatat tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc
     9481 cggtttcaag atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt
     9541 atgcaattca tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt
     9601 tttcattcga tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc
     9661 aagcatcatc aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg
     9721 agctaaccct tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt
     9781 attctttgtc cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac
     9841 aactgggtcc agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag
     9901 cgtaggtgtt ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt
     9961 cattcctttt ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc
    10021 ttttgaaagg ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt
    10081 taataagctc atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc
    10141 gctgctagct aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt
    10201 agggtaagaa cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt
    10261 ttcattcaaa tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa
    10321 ggctagtaaa acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg
    10381 tgcaatttct aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt
    10441 cccatcttca taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc
    10501 ttcatattca ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc
    10561 tttttttgta attatttggt cttcataaag tttttgaata atagcaaaag catttttctc
    10621 actgattgta tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat
    10681 ccttactttt gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc
    10741 acgttggatg gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa
    10801 gaggtctgcc atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt
    10861 tatatatgat tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga
    10921 ctaatattga ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt
    10981 taaataaatc ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatcgacgc
    11041 gcccaagagg agtcgaaccc ctaacctttt gatccgtagt caaacactct atccagttga
    11101 gctatgggcg catattcaat ttaaaaaaat tataaatcat ttgaaaacaa aaagcaagat
    11161 aaaaaaaatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc
    11221 taccaagctg agctacttcc cgtttaaaaa ataaaaaaga c
//
LOCUS       DAZUMV010000088        11245 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000088 DAZUMV010000000
VERSION     DAZUMV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.088"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..1081
                     /locus_tag="ACG8FK_001981"
     CDS             212..1081
                     /locus_tag="ACG8FK_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS5097575.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            1101..1448
                     /locus_tag="ACG8FK_001982"
     CDS             1101..1448
                     /locus_tag="ACG8FK_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097576.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1461..2723
                     /locus_tag="ACG8FK_001983"
     CDS             1461..2723
                     /locus_tag="ACG8FK_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097577.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2803..4179
                     /locus_tag="ACG8FK_001984"
     CDS             2803..4179
                     /locus_tag="ACG8FK_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5097578.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            4176..4805
                     /locus_tag="ACG8FK_001985"
     CDS             4176..4805
                     /locus_tag="ACG8FK_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097579.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            4824..5303
                     /locus_tag="ACG8FK_001986"
     CDS             4824..5303
                     /locus_tag="ACG8FK_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097580.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            5862..6461
                     /locus_tag="ACG8FK_001987"
     CDS             5862..6461
                     /locus_tag="ACG8FK_001987"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS5097581.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            6477..6953
                     /locus_tag="ACG8FK_001988"
     CDS             6477..6953
                     /locus_tag="ACG8FK_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097582.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            6970..7872
                     /locus_tag="ACG8FK_001989"
     CDS             6970..7872
                     /locus_tag="ACG8FK_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097583.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            7945..8136
                     /locus_tag="ACG8FK_001990"
     CDS             7945..8136
                     /locus_tag="ACG8FK_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097584.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            8300..8887
                     /locus_tag="ACG8FK_001991"
     CDS             8300..8887
                     /locus_tag="ACG8FK_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097585.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            8887..9786
                     /locus_tag="ACG8FK_001992"
     CDS             8887..9786
                     /locus_tag="ACG8FK_001992"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS5097586.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            9786..10700
                     /locus_tag="ACG8FK_001993"
     CDS             9786..10700
                     /locus_tag="ACG8FK_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097587.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            10752..10967
                     /locus_tag="ACG8FK_001994"
     CDS             10752..10967
                     /locus_tag="ACG8FK_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097588.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         4321 a         1520 c         2259 g         3145 t
ORIGIN      
        1 ttttttttga taaaaaaact agctattttt ttaaaaataa tttggttcaa aatttatttt
       61 tttaacttga taattttttg ccatcctttt ttaaattggc tatatatcaa ggtttatagt
      121 ggggataaat aaaaatacat ttgacaaaat ttacaatata gtttattata tatttatctt
      181 attatgataa cacaataaaa ggaatgatgg tatgaaaaag aagaagttta gtcgatcgtt
      241 gttaggaatt ttagttttag ttgcggttgg tcttagtggc aagggttact ttcagaataa
      301 cagcactcag gaacatgatt ttcagatcaa aagtagtgtt caggtaggta ataaagcaga
      361 cagtgaatca aaagaatttt tagggatacg ttatgatcca aacaaatatc caaataatat
      421 cgtaaacatt aatgatggga aatatacact gacaaaagaa gaccatgaag cattatcaga
      481 aaaaggcagt caaacctttt gggtaaatta tgcagattta gatcaacttg gaagagcagg
      541 agatgttgaa gcgttgatca cctatgatgc tataaattct cactcatcta aaaatgtgga
      601 acgtccatct ttcaaaagtt ctgtccatat tgcaggagaa tacaaggatg gagtatttga
      661 tcaagagaga gatacttggg tcggcaaaga ttcaaacaat gaaatcactc agctagaagg
      721 atataaaggt tatctctata acaaaagtca ttcattagca tggtcgctag gaggagatat
      781 ggaggtacac aatttaacac ttggaacgag agcgcaaaat gttgggaaaa atgatcagaa
      841 tggagggatg gcttactcag aaacgattgt acgtgatgca atctataaaa ataagaatga
      901 aaaagtattg tataaagttg atccaattta taaagggaat gaattggttc ctcgtggttc
      961 tcatgtcgaa gctttctcag ttaacgataa tggaaaaacg gtaaacctta atgtttatgt
     1021 atttaacaat caaaaaggaa tcaacatcaa ctatcaaaat ggtacttggg taagagatta
     1081 gaaattgaga aggtgaagaa ttggaaacaa taagcatcaa tggattagat aaatcagttg
     1141 caaatcgaat aaaaaaagga aatggctact atcttctgaa gaagggtaaa tattcagttg
     1201 ctagagagtt agaaacaatg agtatcgtgt atgtagctaa gaatcatttt gacgtagaac
     1261 aggctatttt tgatccagat tctgaaatag ggaaatgtgt tgaattatca gagaaaactg
     1321 ttagtacaaa aggggcgatg atcattgaga gagaaatgtt agacgaaatc agagtaatca
     1381 ttcctaaaga tacgcagatg tgtgaggaca gcataagtga cgatgaacca aaatcagaaa
     1441 gaaaataagg agatattcag atgagcgaaa aatccattga attaaaggaa tataagcgaa
     1501 gcgatggaga aaaatattat cctgtagcca gtttttctaa atcagtatat ctggttggac
     1561 gtgcattggt agttggtgca attggcatat atattttgca ttgggtaaca aaggttgtac
     1621 aactgcttgt agatacctta gtaagtattc ttgtctcgtt aaatgatgtt aacggaacag
     1681 atgatttagg attaaaagaa atgtttaaag agttactcaa tgcgtttttt cccaattatt
     1741 taagttattc atataatgta caaagctttt ttgcaaagct acacttggta gacatctcta
     1801 ttttagtatt agaagcagga acggtattct ttgttggata tttggttact ttgatgttca
     1861 ggcatcacta tggagaacaa agaccatttt ttgatgatat ggaaagtaga aggttgaaaa
     1921 gagaagctct agaagctatg gatgtgaagt atgatccaaa caagattata ggagatgaaa
     1981 cgaacaacaa aaaaagaaaa cgaaacaaaa cgcaattaga atccgatgtg ctatcaacgc
     2041 ttaaaaatat caaaatttct gttcatacaa gacgtgatct aagtgtcaat gaagatattc
     2101 gagaatatat cgtaaaaatc ccgcaaccaa aagggaagaa agttagagaa acagttttac
     2161 gacaaattca agatttaggg tcgatcctga caagagcaac aagcggacga gccaaatttg
     2221 gtgaaaaaga agatgaagtt tcaggcgatt atttcatttt ccgagcatca gtaaatattg
     2281 aaagagaacg taaggaaaga gtaagtacca aaaacaaaga aacggaaaga gcaacatctt
     2341 caatcgcagt ttcaaattca caattttctt ttccaatcaa tctctttgcc gataattcca
     2401 aaaatatcca aaaccaaacc gaaaaagcta gaaaatatgc cgaaactcaa aagcaatctt
     2461 tagaacttta tctatcttct gctgacgtac ctgcaagtct aacagatatg cacgttggga
     2521 atacatctat tgaatatttg tttactttat ctcgtaccag aaaaattcaa ctaagctcaa
     2581 tgaaagaaga aattgaaact tacttgcata tatcaggtgt aatcgtgcgg atcaacgcag
     2641 ggcgtattca aattactcta ccattaggag aaaatgatcg tattccaatt gatgtaccat
     2701 caatgatcaa tagcgtgttt taaaagacat cacaaataga aacagaaaat acaaactacc
     2761 agttcacaaa cagaaaagta ccgagaaaag tgaggaatag aaatggctat gaaattatta
     2821 gagaaagtca aaaagagagc cgaaaaagct gaatctattt tgctagataa agaacctaat
     2881 ataaagctta atggaaaatt cagtgtcaat atgttgaaca caaaggctaa agacccgaca
     2941 gatgctattt ttggaattaa gcctgatggc acaattgctc atttttgcat cagcgaaatg
     3001 ccacacatgt tggttgcagg aacgacagga tcagggaaat cagtattact gaatgaaatt
     3061 cttgttacag caatgtgtca ctcaacgcct gatgaattaa aaataggaat tgtagaccca
     3121 aaaagggttg aatttggaag atacaaaaaa ttgccgttca tgttggctga tccaattaca
     3181 gatatggatg aagcttatga tttttttgaa tatcttgtga tcttgatgca tgaacgttac
     3241 aaactgatgg aaaaagcagg gttacaaaac atcacgctat ataacgaata tgccgaaaaa
     3301 aatggattag atagatttcc ctatgtgatc ttattagttg atgaatattc acagttggtt
     3361 ggaacacata aagaagtaga aggtttgatt gttcaattag gacaaatggc aagggcagca
     3421 ggtattcatg ttatcttagc tacacaatct cctcgatcaa cagttgtcac tggtattatc
     3481 aaagccaatt ttccgtcacg tgtttgcctt atggtagcaa gtgatttaga aagtaggatc
     3541 gtattagatg aaggcggtgg agaaagccta agtccaaagg gagatatgat tattaagctc
     3601 gttaatggaa gtatggttag agcgcaggga gcttacatca gtaacaaaga aattgaaaca
     3661 attttcaatc accttagaaa tactatgcca gaaccagaat atgttgatta ccgagcaatc
     3721 gtagctgaat ctcgtggtga gggtggagaa gacgaggatt ttggtggaat tggtaaagct
     3781 cctgtaagaa aatctgctaa aagaaatttg aacacacctt tagcaaattc atcatctaaa
     3841 gagccatcaa ttgaaaagaa gagtacgttt gctctaaaac ttgaaaaaaa caaagaagaa
     3901 aaagaaaaac aaggagctaa aacactagaa aaacttcaag ccgaaaaatt agaaaaagtt
     3961 ttaaaagcat tagataaagc tgattctgaa ccgcaaacct caaacaacac gaataatgaa
     4021 cagccgattt catctaaaac taaaccgaaa actactgatc cgaaagtttc agtaaacgtg
     4081 accatcacaa ccgataagtc tgagaagaaa caagtaaacg gaacgacttc acctatcatg
     4141 caaaaaatgt tgaaagccaa aaaacaggtg cataaatgat cattgtcagt tcttttttga
     4201 ggtggcttac gccatataaa tatacagatg aagtagttgt gaaacgaacg aagaaaagag
     4261 ataaaaaagc ggtactaact cctaaacaga tgcttgatca tggcatttta ggggtgaagg
     4321 ttttagaagt gaataaaccg tttgaatcaa atagtgcaag agaaaagttg cttggagctg
     4381 aagccgatgt cgcatatttt taccatgaca aaagcaagca tcaaatagct attaaattca
     4441 atccaaaatc ggcatggcgg tatcaagcaa gagatgactt gaaacgtagg aaacttagac
     4501 caatgctcta tccagaagga aaagaagcat ttgatagaac ccacatcgtt cccattggtt
     4561 ttcatggtac agaatcagat aaccgcctat tagtaggttg gaacagcaat caaaatagag
     4621 ttcagatcaa taaatttgag aagaaagtaa ggaaattcaa cgatcgaaaa acgatcctat
     4681 ggtttgcaga tatttgtaaa cagccggatg gaacagcaaa gtgggaagcg gtgatatttg
     4741 acgaaaatgg taaaaaagta atgtctgaaa catggcatga taaagctaaa tttgtttggc
     4801 agtagcagta gaggtgaaaa agattgctag aactttttga agcaaataga acaggcttta
     4861 atcaaaagtt agaagtggga gaaactatag aatataaaga taaaaactat attattatcg
     4921 gaatctataa cacgagaatt aacagaatag gtgatccacg tataaccagt gacttggttt
     4981 gtcaatctgt taactattca cctgatctga caagcaggta taagaaatac gtgttattag
     5041 aatatagaca caagattact gatgaaattg gtgtaaacaa cacacgaaac atatttaaga
     5101 tcggaacagt gattccttat agtaacaacg aagaaaaaat attttatcag atttacggaa
     5161 tagaaaagtt tgaatatgaa catgtcgatc tgctagtgac gtaccaaatg cgtttaatag
     5221 aaccttggtc acaggcagaa attgataaag cagtaaaatt gaaccgatta tcaaaattta
     5281 acgtattagg aaatgccttt taaaaatcgc aatacacaaa cagaaaaata ccaaaaaatt
     5341 agcaatgaac gtaggtaaga acatgacaat aaataataat aaccctaaaa aatataatgc
     5401 tcttgtccaa tgctatatcg aaaacaaaat tacaatgcaa gaagtcgctg atgaattagg
     5461 caaatcaaag agcctagtat cacacgattt taagcacaat gtttttccga acccgattga
     5521 gctgaggaaa aaatttcaaa agagagttta tcaagaaata ggtaagtgga ttgatgaagg
     5581 gaatgcaatt gaccctttaa ttgaaaaata tcctttttta aaaagcaaaa ataaaattca
     5641 aaggttaatt cgtcaaggcg atattcaagc taaagaacct gttttgacaa ccaatgattt
     5701 ggctaaaata aatcttcata aacaaattat tgaagacttt gaaaaaaatc tttcaacaac
     5761 tgaaatttta aaaaaataca atattccgag aggaaagcta acatcgactc tttttagaga
     5821 atatggagaa tcggcagtta ctaaagcaaa ggaagtagcg aatgagagaa aaaaacaaaa
     5881 atagtagtcg atacctagta gcgactgtag tttttgtttg tgtattgatt ttcctgtttg
     5941 attttttgca gattggaaaa agcataattg gaaatacatt gagtgcaaag gaaatggttg
     6001 ctttaggtgg tttgtatgaa ggacacttgt tcccgaatat attcatttct atcgtttctc
     6061 atattaactt gtaccattta atttctaatt tattgttctt agtagttctt gaagaagcac
     6121 tgaatgatca gtatgagtgg ttaacattat tgatcgtttt aatcatatca ggatttctag
     6181 gaagcttagt tccttttctg ctacacagca acatcgtaag tatggggctt agtggaggta
     6241 tttttggatg gattgcttta tttgcgattg catacatccg ttttgatagc aagaaacatt
     6301 tattactaag tgtttttgta ttattgataa tcggcatttt actggtatct acttttgaat
     6361 caggcgagac aaacgtcttg tcacatgtaa tcggatttgt ctgtgggatg atcttgggat
     6421 ttattctaaa gaacgtttca ttgatcaaac aaaagaatta aaagggagtg ctaaaaatgc
     6481 caaaaaaaaa agaaaagaaa attccagtgt ttttgtctgt ctcagaaatg ttatcacttc
     6541 aaatactgtt aagaaaaaag attgaaacaa tagacgaaga agttagaaat aaaagggcta
     6601 tgtacgaaca acgtgaagcg ctaagagaac gaatccacta ttttgatgat atacacacta
     6661 aaatctatga agcacgtaca aatcacgaag acgaaagcaa attaggatta gctcaacaac
     6721 gaatatttga atggctgaat acgaatactg agcaagaacc gttaagagca attagtaatc
     6781 tttttgaatt tggaacgaag cattggttta acgctgtaga aaaggatgct gacttacaag
     6841 aagcgttcga tacactaagc gaggatagca aatccatgcg agacttattg atcacttatc
     6901 taaatgagca tgaatttgaa cagacgaaag ttaaaaagaa aaataaaaaa tagaaagttg
     6961 tgatgaatca tgctattaac cctgttaggt tatttatgtt taattcttgc tttttttggg
     7021 gcaatcgcta taattattgg tcaatttcaa acaaataatc cgattatgcg taacataggc
     7081 attggaattg tgattgtaac ctttattgtg tttaatgcca ctaagatttt tggaagtaac
     7141 gataaacaat ttggtatatc gggagttaat aatagttatg ctccagattc tttgggaagg
     7201 gtagtaatag atggaacatt tgaaaatgta acgagttata aaaatgtttc gctgacagtg
     7261 aaggaagatg gcaagaaagc atcagacgac gttctcgcta ctattaagcc gtctcaatca
     7321 caatttgaag tgttttatac agttaaagag aatagccctg tacacaagct gacattggtt
     7381 tttaacgttg atggagaaga atactataaa gatgtaaaaa taaataaaac atcaacgaaa
     7441 aagattatcg aatcagcgaa aaaagaagta gctacagaag aaagtacaac tgaatcaagt
     7501 tctactgtcg aagcggaact accagataaa attgattttg atcctgaaaa agtcaaatcg
     7561 tttgaatctg tttctggttc tatggctgat cggtttaaca ctgaagtctt caaagctatc
     7621 aaaactgtta aattggatac tgaaaagcaa atgattaatg ttaccttatt caattcagtt
     7681 gaatctatgg atcaggataa aaaagttacg gttgcaagag aactaggaaa aagagtaatt
     7741 ggtttgtatc gtggtactgc taaaccagat tcagacggtg cagatttgcc agttcatgta
     7801 gtttatgaag acggtaaagt ttttggagat tctacagcaa acgaagcatt aacgattaaa
     7861 ttggctaaat agccagaaag ttataataaa caaatggtgt cacaccaata atatataggt
     7921 gtgacacctt ggagatgata gctgatggta gtaaataaag aaaaagttag aaagtatgtg
     7981 actttaaaaa tagcttctgt taaatattta gaaaagcgag ctaaagaaga agaaagagca
     8041 agaggtaaga aagtaacagt ttcagagttg ttagaagaat tgatcgagaa tgatcaaaca
     8101 ataagacgtg catttactaa taggtctatc agataacaat ataattaata aaaaagaaag
     8161 agtgatttgg tcttagacta aatcactctt tttatataaa gtttctttac actcattccg
     8221 aataatgtta aaattcttta tatgatatat agttccttgt cagaggaaga gataaccagc
     8281 aatattggag ggataataag tggaaaacaa taatgatatt agacatttta gcattgggga
     8341 aagagttaca acagtaagtg aattatcaaa ggattataga gataaagagg gaacgattat
     8401 ttctcttaat aagaatcgaa gaggcgtttg gcaatatgtt gttgatttag gtgatggcgt
     8461 tacagttgag tgtaaagata gtcaactgat cagaaataat ggcagtcatt ccattgaaaa
     8521 agcaattctt gtatacccaa aagtatcaag taagatcatg aatgactggt tgcttggaaa
     8581 atttggtgag tacaataaaa aagaaatcgc agaagtgatc ggcaaatctt atccaacagt
     8641 attagggtat ctaaaaaaga taacgagtaa agaagcaaaa attgaggatg ttttaacagt
     8701 aagagagctt caatcattaa tcgagtatcg gaataaacaa ataacaaatc ctgagtacct
     8761 tagaaaagaa gatggtctat tgaaacaatt ggaacaagta aataaaatcc ggaagatgca
     8821 aaaggtagct cctttaagcc ttgatacctt taaaaaaatc tatttgaaag agatcaaaaa
     8881 agactaatgt ggactgtaac tttaccgttc caacctgtat ctgcaccaag accaagtgca
     8941 agaaggaatg ctgctgatga aactaatgac gtagcagaga aaaggatttc aacctactat
     9001 gataaaaagt atcttgatta tttgaaaaaa ataaagagct ttatcgaaga tcaagattta
     9061 ttagatgatg aattttactc gattgtaaat gatcctatgg gcgcaattat ggaagtggac
     9121 ttttactacc agataccaaa aagctataaa aaagtctata acattatgaa gacaacagcg
     9181 ccagatttag ataatttagt aaaaagtgct atggatggta tttttaacat ctcagctata
     9241 cgagatagcc atatcacagg cttaattgct tttaaatata atgtggcgaa cgaaggaaaa
     9301 acagttgtga ggatcaaacg atattcagaa tttgagtggg atacttctga aggattgaac
     9361 ggagatatat gggaagctac atttgatttt aaaccagtag caacgccaag accaaagtct
     9421 aaatttagag gaaatgggat tatcacttac tatccaaaag agtacaacga ctatttggaa
     9481 aatatcaaga atacgcttaa agagaaagat ttggtaaatg atcaactgaa aaaggtaatg
     9541 aatgctccta tgggggttat agctcagata gattattttt accaagtgag aaaagataag
     9601 aaaaagcttg attcactgat gcgtacttca cagccggata ttgataattt agtcaaagga
     9661 acattggacg gaatttttaa caaggaaata ggcatcaaag atagcagggt tgtagggtta
     9721 attgctttta aatacaatac ttttgaaaag tcgaaaacaa acgtgagact acaggagatg
     9781 ggctgatgaa taataatgaa aaaattcttc atgtacttga tagctttgaa ataattcaag
     9841 aagaactaaa aaaatatcgg gatgttttgg aacaaagata tgattttgtc aatcagcaaa
     9901 agagtaatca catggatttc attctcaata tgaatgactt gaagaagaaa ttagttgaac
     9961 gaaaagaaca agaaaagttg atcaaagctt actttgaact cggtgaaaaa gaagttaaaa
    10021 atgcaatgga actgaatgaa gagcgcaggg ttttagatca gctgttagaa caattattgg
    10081 tgatgtttca aaaaggacga atagatgagg atttaattga agaaggattg agaaaatatc
    10141 cttcaaactc aggtatcgga atagtgctga aagcgattga tgaagaagaa attgaagctt
    10201 tcataccgcc agaggatttt gaatcggcaa tggaatatat aaaatattat agccaaggga
    10261 tcactgcttt tagagagttt gatcctgaag atgttattca tgacttgaac aacctaaaag
    10321 aatggtgtga aagttatgga gtcgatgatt ccggattgga ctacttgatc agtattatgg
    10381 aaatcgaaga agagatgccg gataagcctg atccaacaga tattttagaa ttgatccatg
    10441 aagctagaaa tccaattgcc tatatttcta gaggatatac tgtattggaa tactataagc
    10501 cttatatatc agcgatgaac catttaagac gtgtattaag ggaaaaacgt gaataccgct
    10561 cagtgttgaa tgcaacgaat agattagaaa aagctgtttc agagctagat gcttattatc
    10621 gtgaacatta cctgcaagca ggtggtatgc caagaaatac gaaagcaaat atcagtaggt
    10681 atataaaaaa agctgagtag gcggagttag cccataaaat ggcaaaaggt aacctgaaag
    10741 gggcattttg aatgaaaagg gatgtttttg aattgtacgt attagaacgt ctagaagacg
    10801 gcatgatggc acaagagata attgatgaaa ttgatgatat gatcagcaga tttggtcaat
    10861 tggagttgaa tgatggaaac aaaaattcgg tagtgctaaa acaattttgg ttgaacaatt
    10921 tttcgtataa ggattttatc aaagaaaagg caaatacatt gtactgacta aattcacgag
    10981 gagcagtgat gagagcttat tgctaagagc agttaggctc ttttttgttt gtgtaaaatt
    11041 atttgtttta taatacacat ttatacacaa aaaaatattg acagatagta cacaaatgtg
    11101 gtaggatata tttgtaagca agttagatta acaagttaaa ggaagagcag ttatgaaaaa
    11161 agaagaacta atagatatat ttcaaattgt tgaacgagca aataatatgg gaattatgtt
    11221 ttttgatcgt atttctttaa agatg
//
LOCUS       DAZUMV010000089        11186 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000089 DAZUMV010000000
VERSION     DAZUMV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.089"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(484..861)
                     /locus_tag="ACG8FK_001995"
     CDS             complement(484..861)
                     /locus_tag="ACG8FK_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097589.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(1161..2042)
                     /gene="galU"
                     /locus_tag="ACG8FK_001996"
     CDS             complement(1161..2042)
                     /gene="galU"
                     /locus_tag="ACG8FK_001996"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5097590.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(2085..3104)
                     /locus_tag="ACG8FK_001997"
     CDS             complement(2085..3104)
                     /locus_tag="ACG8FK_001997"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5097591.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3737..4741)
                     /locus_tag="ACG8FK_001998"
     CDS             complement(3737..4741)
                     /locus_tag="ACG8FK_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5097592.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4754..5803)
                     /locus_tag="ACG8FK_001999"
     CDS             complement(4754..5803)
                     /locus_tag="ACG8FK_001999"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5097593.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5820..6641)
                     /locus_tag="ACG8FK_002000"
     CDS             complement(5820..6641)
                     /locus_tag="ACG8FK_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5097594.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6628..7407)
                     /locus_tag="ACG8FK_002001"
     CDS             complement(6628..7407)
                     /locus_tag="ACG8FK_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5097595.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(7424..7894)
                     /locus_tag="ACG8FK_002002"
     CDS             complement(7424..7894)
                     /locus_tag="ACG8FK_002002"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5097596.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7906..8325)
                     /locus_tag="ACG8FK_002003"
     CDS             complement(7906..8325)
                     /locus_tag="ACG8FK_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5097597.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(8406..11183)
                     /locus_tag="ACG8FK_002004"
     CDS             complement(8406..11183)
                     /locus_tag="ACG8FK_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5097598.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
BASE COUNT         3222 a         2374 c         1849 g         3741 t
ORIGIN      
        1 aggcattgaa cgaatacgcc caataccttg acatagcaaa taaaacaacg tgcagaccat
       61 ggaaaatatc tacatagggg tagaattttc accctagatt aggtacaata aattaggtcg
      121 atgcactgca ttgactatgg caaaatgatc ggtcagggtg tatgtgtgtg actggtcatt
      181 ccctgattct tagcgccaac taagggtcag ggagcctttt tttattcagc tgtttctatc
      241 tgaatttatt gtaagtaatc tcttatccaa gtgcaagcaa gaaaatacaa aaaaacattg
      301 gtatgattta tatatatata aataacataa aaaaaggaat ggcttatccc gatacaacaa
      361 gcattccttt aaaaaagatt cttttatata agtgaaaaaa gcttttttcc accaattaaa
      421 taaatctctc atctcaatag tttatccatt attttatctt tttttgcgta tacctactaa
      481 atattaccgt cttcttctcg ttcttctact ttgttcgaga gaatctgtac tagaaaaagt
      541 agttgtatgt ctaccttttc tgcctatccg agactttctt ttcatatatg ggatataaat
      601 gattagatac agtacagcaa aaaataaagc aaatacgcaa gcttcccaac tacctaacgc
      661 caattcaacc atcaaggtag caaccccaac ggatgctgca taccagataa aaatcagaaa
      721 cgtttctttt tttctgacca tgcgaaacat gataaataga ctaataagca atagtggcaa
      781 cacaaatatc caattgcttc cttccatcag ccacttgagt atatcaagca gcgactgtgc
      841 aacagcgact aattttccca tagtgaacac acaccttttt ttataattaa aaaattcgcc
      901 tttttattct actatgaatc ttctggcttg gctttcattt ctcttatgta atcattttct
      961 tttatacaac ttaaaaaaaa attaaaccat tagcataata ttttctgaat ctattcacga
     1021 aagtcttttt ctatctttcg ttttccacga ctcaactgat ctagagtcaa aaaactaaaa
     1081 cgacagttgc gcaaatgacg cacaagctgc cgctttaata acaaatagcc tttcattctc
     1141 agcaggccac ttatttccag ttaactttct gttcttagct ttttactcaa ctggataata
     1201 tatgcactta atgaagtccc aatttccgaa tgttgaagtc catattcaat atttgtcttc
     1261 atatatccta acttatcgcc tacgtcgtat ctttttcctt taaactctct cgcatagacc
     1321 gcctctgtct ggttaagagt ctgaagtgca tccgttagct gtatctcgtt tcctgctcct
     1381 ggttcttgag tttctaatat ctcaaatatt ttaggagtca gtagataacg tccgataatg
     1441 gctaaattac ttggtgcttt tcccatttca ggtttttcta caaacccttt tactctgcaa
     1501 cgaccctttt cgctatctgt tttaggatca ataattccgt atttagatgt ttcattttct
     1561 ggtacacgca tgacggcaac agcggaagct tgtgtttctt cataatccat catcaattgt
     1621 tgagtcaatg ggatatcatc tttcatcaaa tcatccccca gcatgacgac aaacggttca
     1681 tttcctataa atgcttttgc ttgaagcacg gcatccccca gtcctcttgg atgagattga
     1741 cggatgaagt gaagattgac ttgagtcgtt tcttcaacta aagcgagtaa ttctgttttt
     1801 tccttttccc gcaaattgtt ttccaattcg atatttgcat caaagtgatc ttcaatgggt
     1861 cttttttctt ttcccgttac gatcaagatg tcttcgattc ccgaatccaa tgcttcttcc
     1921 acaataaact ggatcgttgg tttatccacg attggaagca tttcttttgc catggctttt
     1981 gttgccggca aaaaacgtgt tcctagccca gcggcaggaa tgactgcttt tttgaccttc
     2041 acttgctgtt cctcctaaac gacgattaac tattgatcat ctatttaacg aagagaaaat
     2101 tcattctcca tttttccgtc acgtaacatg atttcttttg ctgctttttt gatatcttct
     2161 ccattataca aaacattata gattgtttga gtaattggca tctcaacacc tagctgttga
     2221 gctagctcta ctgctgcttt tgtcgttgcc acaccctcaa cgaccattcc catgttttct
     2281 agaacttctt ccagcttatg tccctgtcct aacaagttac cggcacgcca attgcgagaa
     2341 tgaacacttg tacaagtaac gatcaagtcc cccacaccac tcaaaccgat aaatgtcaga
     2401 ggattggcac ccatcgcgac accaagacgg ctgatttctg ccaatcctcg agtcatgatt
     2461 gctgcttttg catcatctcc gaatcctaat ccatggatag cgccggcacc aattgcaatg
     2521 atatttttca gtgctgcacc agtttctacg ccaatcacat cattatttgt ataaatcctg
     2581 aaataatcat tcatgaacag tttttgtaca taggctgctg ctttttgatc aatacttgca
     2641 gctgtaattg ttgtgatatc atgtactgcc acttcctcag catggctagg accagataat
     2701 acaacaattg cctgtctttt ttcttctgga atttcttcag ccaaaacttc tgagattcgt
     2761 ttatgcgtat cttgttctag tcctttactt gcatgtataa tgattggctt agtattcatt
     2821 ttaccaataa gttcctgtgc gactgaacga atcgcttttg ttggaacaac aaataaaata
     2881 gcatctgcgt tatccactgc ttctgccaat tctttatatc cgataatcga ttctgggatc
     2941 gtcaaatcag gtaaaaaatg ctgatttgta tgatacttat tgatttcatc aatctgttca
     3001 ggtacatttc cccaaatacg gacatcatgc ccgttttctg ccaatgtttg tgctaaagca
     3061 gtgccccaag atccaggacc aagaactgcg attttttgtt tcatggatat tcctccaaat
     3121 aaataattga taatttccat tatagtatac atttcttaaa gttgggacta atttccattg
     3181 tttttaagtg tttgtttact tcttttatcg tttttacttg aaataatcaa tgatatgtta
     3241 caatagtcta cattttattt ttacactcgt tatgctttgc ttgttcctaa gaggaagcag
     3301 ttttgacaat gttactacca gcattactat taggaggtta ggaaacttct cctagtgagt
     3361 attgatttaa aatacataag agacagattg aacatttcta aatctgtcag gtcctgcaac
     3421 ggacagattg tctctgttga cggttgacat aggaactttt gttttagtca acattctgga
     3481 tacaaaacgc tatgtaaact ggcgcaactc gttgtacttg gacttttact acggatccat
     3541 gcaacaacta aatagtacta ttgcataaca aaaaatggtt ggcacccaca tgtcccatta
     3601 aacagattat aagttgaact gtagataatg caaaaaatgt gttttataac gtaattgatg
     3661 ccaatatttt aaaaaaataa agggagcagt tatcgctgct ctctttattt tttatattct
     3721 ttttatccag tctatcctaa taatcaaact gacgatagta gcggcgtatc tctaatggat
     3781 ggcagttcaa ctgctccaca tgagcatcga tgcgttgtgt cactacatgc atcaataaga
     3841 aagacaaaac acgtccgcga tatttttcac tgataccttc tactggatag tcttttgtgt
     3901 ctatcaatgt ataattgctg caaatttttg gcaaaagttt agccacacgt tcagataaca
     3961 ctctctgttc atcttcgcct aaaaataatg tgactggggt tgtttcttct acgatctcta
     4021 acgtcccgtg aagaaactcg gcagcatgga tagatttcga tggaagccag ctttgttctt
     4081 cccagtaaca catggcatat gaataaacag ctccccattg attccctgct ccaataaaat
     4141 aatgcatact atcacgtcga tgtttttctg caaatgataa gccaaatgca tctgcttttt
     4201 tctctgcttc cactaaccct gttggtaaat aacgttcgag ctgttcatag tattctgaat
     4261 aagcttcgaa ttctccattc aaatacatca atcgatctgc tgccataaaa aatttgattt
     4321 gttctgttcc aggagctgga taagtgacca caaaatcgca tagctgagac aacttactgt
     4381 tcgctttatc gataaatcca atcagtgtcg ctcctacttt cttgatttgt tctactgctt
     4441 tcactacttc ttgagttgta ccggtaacag aggacaaaat aacgatagaa tcttttgtta
     4501 atcttttgtt tccagtagtg taatattctg cagcatgctg tacaaagaca ggcaagttgc
     4561 ttttcccatt catataagtg acagcttgca tcgaggaagc atacgttcca ccgattccta
     4621 gataatagac agcgtcaaat ttttccgcat aaagtttatc gatgatttct tctacttgcg
     4681 gacgtaaagc tagtgcgcct tttattgctt tgatttgttc ttcttcattg aatttcagca
     4741 tgatttccac ctcttataat ttacttttca agactgtatc aaagtcttca aaaatctttt
     4801 tgttcaagtc aataccttta gctactgcgg ttttataagc gaatagttga aacggaacga
     4861 caagcgctaa tacagacaga tattcatcac attctattgc taatccaagt gttttctcgt
     4921 ttcttgcttt ttttgtggta atggtgagag tctctccaac gtaccgactc atatatcttt
     4981 gcaacttttg cgaacgttcc ttatttacac tttccccttc aataaagaat agcaaatgcg
     5041 aagcatctgc ctccaggtat gggccgtgca tataagcttc taactcaaat ccttgtgacg
     5101 gaacgcggat tgtttcagtc aatttcgtct ctgcttcttt agctgttccc cagtttggac
     5161 catatccaat ggtgacgaaa cgctgtgcta aacgaaataa actttgatat ccatcaaaaa
     5221 attcttccgt tttttgaata atcgctggta aatggttgat tattttgcgc aactgttgca
     5281 tgtagtcttg ttctattttt ttgctgattt tatttttact tattccaatt cctatcccta
     5341 agagtagtaa ctgtaaaaca gtcgcagaat agccttttgt aacgaagcct actgattcta
     5401 ccccggcacc taaatcaata atttgtgcag cttctaaagc aagtggactt tgcacgtcat
     5461 ttgtgatagc aacaacaggt aacccttgtt tttgtatcat ttttactgct tcaacagtag
     5521 aagcactttt cccgctttgt gacacagcaa tcacagtatc gattgaagga gaaagttttc
     5581 cataattgaa aaaataaaaa ggttcttgaa tatcgacagt aagatcacca taagattcca
     5641 atgctggctt cgctgctaaa caagcgttat aagatgaacc cgttgctaag atcagtaaat
     5701 tcactgtttt tttcatatgt tctcttgtcg aaaaatcatt ctttttcaag atcgtactta
     5761 gtacttcctt ttcttcatag atataatctt gcatcgtaaa cattttctct gctccttttt
     5821 taaaaaatcc ctatccacga accaaaaatt ccaaagaaag ctagtccgat caagatcgtt
     5881 aatggtttca cttctttctt caatagataa aacactatgc caaatgctcc aagacataag
     5941 atatttggaa cgatatcatc aaaaatactt tgaacagtca cagcattttc tcctgaaccg
     6001 atttttaaag gaatattgat ggtgatcatc gtggcagtca ttgctccaac gaccatcagt
     6061 ccaataatag acgcgcctaa tgtcaaactt tccatcatac cgtttgcctg gatctttttc
     6121 aaaaaacccg ttcctaagcg atatccccaa cctgttgcta gatagcgaaa cagaatgtgc
     6181 ggaatattga aaatcaaaat gaacaagata gggcctaaaa tatttccttg catggctaaa
     6241 gaggtaccca cacctgtggc aatcaatcgc aaagttcccc aaaagaaaga atctcctaac
     6301 cctgcaagag gtcccatcaa agatgttttg atactatcga tcgcagtcac atcaaattca
     6361 ggatcatcct tattttcttc ctccattgca gccgtgattc ctaaaatcag tgtaacgata
     6421 tgtggggtcg tgttaaaaaa ctctagatgc cgtttcaaag ctgctgccat ttcttctttc
     6481 gactgatata actttttcaa aatcggaatc attgcataaa cataagccat attcatctga
     6541 cgttcataat tccaagaaaa ctccatttga aaagaacgcc aaaatacgtg atttagctct
     6601 tttttcgtta ttttgctgtt tttgatctca gaattcttca tcttcttcta cctcccctgc
     6661 agttgtttga agtggtagtt gacggacatt cataatattg actaccacaa ctgctaaaat
     6721 cgcaccaaaa atagctactc cggtcacagg aatctctaag taagaagcta tagcaaaacc
     6781 taagaaaaaa tatggtgcaa cttgtttgtt gatcagcaaa cgagcaagca tcgcaaaacc
     6841 aagagctgga atcaaccctg tagctactga caaccccttt tgaacgaagt caggaatcat
     6901 atttaataga tttccgatcg tattgcttcc caccatataa gaaaccatta ccacaacacc
     6961 aagcatcaag gaaagaccga taccggcaag caagtgcata tgctcgactc ctttataatt
     7021 tccttcttct gcataattgt ctgctttatg gttcataatt ggaatcaaaa gccctaagta
     7081 aatatttttc aatatcaatg tcaacgtagc aatcggcaaa cctagtaaaa gcgcggtttc
     7141 tgtaccggct ccagctgtaa tagcaaaagc cgtccctaaa atcccaccgg tcacaacatc
     7201 aggtggaatc gaagcaccta ctgagaacga tccgataaat gctaactcta aagttgcgcc
     7261 cataatgatc ccagttttta catcacctaa cacgattcct gtaaacagcc ccgtcacaat
     7321 cggacgagaa agcaacgatg tccctagtgc atattctgat tgtgcgacaa atgcaacaat
     7381 tcctaataag actgcttgta caatcattga ggttcttcct ttcttacaaa acttctctta
     7441 catccacttt tttatctgct ggtacttgac ggatttctac ttctatttgt ttgtcaatta
     7501 atcgtttcaa gcgttcctcc tcttctggta aaatatttac cgcttttccg atgctgcgag
     7561 taccaggttt tgccttgatc cctcctaagt tgatggtttt gatttctgga tacgcttctg
     7621 ccagcttata tgcatcctcg atcgattcca ctacgatgaa taacttatat ttatctgtca
     7681 ccccgctttt caacgcggca atcgagtcat caattgattt gatcactagt ttgactcctt
     7741 gtggcttggc taatttgatc gttgtctttc gtatctcatt cgtaggtaca tcatcattag
     7801 caatcaaaat acagtctgcg cctattgttt gtgtccatga aaaagctact tgtccgtgta
     7861 ataaccgatg gtcgactctt gtcaatacaa tcatttttct tcctcctaaa attcgtcttc
     7921 ttctattttc tgattgagcc gctcgttaca aaactggatc attttttttg aatcagttag
     7981 cacctgctgg atcaaaacag ttgtctctac ttcagcgtcc aaccgagata ctagctcaat
     8041 caatagtggc aaattcaatc cggcaacgag atagacatga tcccgattga tatattgtaa
     8101 gaattcgttg ttgacacttc cgccaaaaag atccgtcaga acaatgattt ctttatcttt
     8161 gtacctcata aaaagctgcc ttactgtttc cgctaaatca aaattctcat cagtatagca
     8221 atcgaataca gtaatagaat gatttttccc atagatcagc tctaatgaat ctaatatccc
     8281 tgaagcaaat tttccatggg aagctaagat aatttctcta tccacttttt catctcctct
     8341 tttcacttac acatactaaa aaagcaagtt gcatgccaaa attagtggca acaacttgct
     8401 tatgttcaaa attcttcttc tatcgagaga aactctgtat cttcactcaa aatatcatag
     8461 atataggcaa tctcagaatt ggggatcatg acactataat gctcttcaat gacactaaac
     8521 gcagatttaa tagcagataa cccttctttt tgacactgct tccatttctc taatccttga
     8581 taggtttcga ttggcatttg tcggatcaat cgttcaatga gacagctcac atggacatac
     8641 aacgctaatt ttttgggatt gctgatgctt cgattcaact gcacctctaa ttctctcata
     8701 aaatgatcga tttctcccat caccttctct gtatctagaa tagtcacgga atccagtact
     8761 ttttccaatg aaaaattacg aatgatgcgc tgattgaata tttccttctc tgaagcattc
     8821 atagtagtgt ccaaccattc agctagtaca gacatctttt cccccgagat gaggtcttct
     8881 aatgaaagat agtcgatatt ggtgatatca ggatctgtgg tgccaatcat tcctacgact
     8941 tcgtatatgg aaaataatgg gtcactgttt ttcttatctt ttaattgctg atattcatat
     9001 gggatgattt tcaactgaca agccgtaggt aagctttttt ctagcagttc acttaagtgg
     9061 accgccgtac cgattccagt gaagcaagta gtaatgagtg ctttgggttt gttttcctct
     9121 ggatagagca gtcgccattc tggctggatc tgtcgaacag tctcttctgc tatctcttct
     9181 aatgatacat ttttttgcag gcattcccct actgtgattg ctaaaggtgt cgtcacatta
     9241 ttcaaaatca aaagcggtac ggataactgt tttttaaaaa actgatggat ttctttcaga
     9301 gatcccatgt ctactaatat gatcaatcca tttgaaacat cgctgttttc gcaataatca
     9361 aggatttctg aagcaatctg ctgtggtgtg acatctagtg gcatatcaaa tgattcaaat
     9421 agatcctctt gtaataaacg atttactaca tttgccatac tgctagcagt tgcataaccg
     9481 tgagcagcaa tcatcgcttt tggcagtcgt ttttcttttg accatttcgc tttttttaga
     9541 tagatcgtca gtaaaatccg atccatactt gtgtcttcaa tatctaattt gatcttactt
     9601 aattccagaa ttcgttggac atagtgataa ctagatggat aagactgaga aacttgttgt
     9661 tctaattctt tgattagttg gataatatca tgttcttccg gtaaccattt gatagtgctt
     9721 cgctgataga gatagtaact caaggcatag acactgtttc catcgaattt gatttgatag
     9781 gcagtttcca tttgtcgaaa ggtctctcgg atagattgtg tccaataaac cagcagctcg
     9841 tgtttttctt gtctactggt ctcaaacagc aaaaaatcaa ataattgaat cacttcttgc
     9901 tttaattttc cttcgcaaga atatagcaga cagttatttt tcttgaaatc aattaggatt
     9961 tgctcaaaac ttttaacgat tcgctgctgt tcgggctgtt gcttctccat taattgctct
    10021 aaggtctggt cttgcgtaat atagattcct tgctgtgctg tatttttagt aataggctgc
    10081 gcaagtagtt ctttttgcaa atgatggatc gtgacatgga tttcaggctg ttcacgctgt
    10141 tcagcatatg cttttgctgc tgaaactttg acgatatttt tcagctctcc tatgtttccg
    10201 gataatttct gacttgttaa aagtgacaaa gctggtccac tgactgacaa agggctcccc
    10261 atttttcttt gttcttcgag aaaaaaagag taaatcagtt ctagccgttc attacgacta
    10321 cgctgattaa gtgcaggtag ttcgattcgg ataggtattc ttcgaatgaa agttgttaag
    10381 aaatgactag taatctcttc agtcgtagca aaaaagagac gtgttttgat ccggcgtgac
    10441 aaattaggtt ctcccatacg ataaatcaca ccttgatcaa gataggtgaa cagtttttcc
    10501 tgaccttctg gtgttaagcg atgtacctcg tctaaaaaca agatcccacc atctgctgct
    10561 tcaaaagccc cttttttatt ttcttctgca ccagtgtacg ctcccttaac atgtccaaac
    10621 aaattgctag tcaaaagttc aggattatct gcatattgtg cacaattaaa agtgatgaat
    10681 ggtgcagaat cttctagcag atcatgaagc agacaatact gatagaccaa attaactaaa
    10741 taacttttac ctgtgccact ttcgcctgta atcaaaagtg gtaatccgcc atctggatag
    10801 taaagagctg ttttgatttg ttcgatactt tctttcaaac tggcgtcgta tccgatcata
    10861 aaagagaata agtcttttgg ttgcgcgaaa aaaggctgtt cctctttgag atcatcgata
    10921 gaaaaataaa aattcgtgcg taacttaaaa aattgttttt caaaagcttc tttgtggaaa
    10981 taaatcactg gtcgagaatt gattttgact agtagttgct catcatttaa ctggttcaaa
    11041 taatgactga ctgtgtttct ttttacccca aaatgatcag ctaacctttg tgctgtaaag
    11101 acctcgttca tatgttctaa atcgaaaaag gctgtctggt tctctaagta acataaaatt
    11161 tccttcttca acaacttatt cacccc
//
LOCUS       DAZUMV010000090        11182 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000090 DAZUMV010000000
VERSION     DAZUMV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.090"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8FK_002005"
     CDS             6..305
                     /locus_tag="ACG8FK_002005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5097599.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8FK_002006"
     CDS             378..1739
                     /locus_tag="ACG8FK_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5097600.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8FK_002007"
     CDS             1761..2021
                     /locus_tag="ACG8FK_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097601.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8FK_002008"
     CDS             2035..2787
                     /locus_tag="ACG8FK_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS5097602.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8FK_002009"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8FK_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5097603.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8FK_002010"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8FK_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS5097604.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8FK_002011"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8FK_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5097605.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8FK_002012"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8FK_002012"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5097606.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8FK_002013"
     CDS             7161..8558
                     /locus_tag="ACG8FK_002013"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5097607.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8FK_002014"
     CDS             8639..9079
                     /locus_tag="ACG8FK_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5097608.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8FK_002015"
     CDS             9227..9553
                     /locus_tag="ACG8FK_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5097609.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8FK_002016"
     CDS             9554..10303
                     /locus_tag="ACG8FK_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS5097610.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8FK_002017"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8FK_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5097611.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3552 a         1958 c         2295 g         3377 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaaatta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tg
//
LOCUS       DAZUMV010000091        11165 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000091 DAZUMV010000000
VERSION     DAZUMV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.091"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1398)
                     /gene="feoB"
                     /locus_tag="ACG8FK_002018"
     CDS             complement(<1..1398)
                     /gene="feoB"
                     /locus_tag="ACG8FK_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS5097612.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFS"
     gene            complement(1395..1868)
                     /locus_tag="ACG8FK_002019"
     CDS             complement(1395..1868)
                     /locus_tag="ACG8FK_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS5097613.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVSL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            2269..2493
                     /gene="nrdH"
                     /locus_tag="ACG8FK_002020"
     CDS             2269..2493
                     /gene="nrdH"
                     /locus_tag="ACG8FK_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS5097614.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            2652..4811
                     /gene="nrdE"
                     /locus_tag="ACG8FK_002021"
     CDS             2652..4811
                     /gene="nrdE"
                     /locus_tag="ACG8FK_002021"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS5097615.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            4861..5826
                     /gene="nrdF"
                     /locus_tag="ACG8FK_002022"
     CDS             4861..5826
                     /gene="nrdF"
                     /locus_tag="ACG8FK_002022"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS5097616.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(5915..7054)
                     /locus_tag="ACG8FK_002023"
     CDS             complement(5915..7054)
                     /locus_tag="ACG8FK_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5097617.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(7363..8076)
                     /locus_tag="ACG8FK_002024"
     CDS             complement(7363..8076)
                     /locus_tag="ACG8FK_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS5097618.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(8330..9031)
                     /gene="nagB"
                     /locus_tag="ACG8FK_002025"
     CDS             complement(8330..9031)
                     /gene="nagB"
                     /locus_tag="ACG8FK_002025"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS5097619.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            9265..10797
                     /locus_tag="ACG8FK_002026"
     CDS             9265..10797
                     /locus_tag="ACG8FK_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5097620.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(10988..>11165)
                     /locus_tag="ACG8FK_002027"
     CDS             complement(10988..>11165)
                     /locus_tag="ACG8FK_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5097621.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         3610 a         2230 c         1958 g         3367 t
ORIGIN      
        1 agagaaaata attgcagtca taccaagaaa ataggcagaa ggagctaccc aagattgatg
       61 aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac tttgcagaac aaggcatgaa
      121 ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt tcaatcgtgc gactggccat
      181 gacgcctggc actccacatc ctgtagcaat cagcatagga atgaaggatt ttcctgaaag
      241 accgaatttc ctgaaaagtc gatccatgac aaaagcgatc ctcgccatat atccgcagtc
      301 ttccagaaag ctcaagcata aaaacaatat aatcaattgc ggtagaaatc ctaacactgc
      361 tcccacgcct gcgatgatgc cgttcaagat cagctcctgc atccatcccg ctacttgcca
      421 ttcagctaac aatctgccaa catgttctgg taaccatgta ccaaacaatg tgtcattgat
      481 ccaatctgtc cccattgttc ctactgtttg aatagccaga taatagatca gccacatcac
      541 aaatgcgaag atcggcaagg ctaaccaacg attcgttgca atctgatcga ttttgtcact
      601 catcgataat tggaaagaat catttgacac aacacagagt gtacatagtt gggtaataaa
      661 ttcataacgt tcattgacca gaatcgtctc actatcgtcg tccaatagtt tctctgttaa
      721 ctggatcagt tgctcgatct ctttttgctg agaaactgct atctcgtaat cctctcttac
      781 cttctgatct cgttcaaaat atttcattgc ataccagcgt ttcttgccgc tttgttctcc
      841 tagtacatct tcaatttcat gtaaagctgc ttctaaacgt ggatcatatg tacagggaac
      901 aggaatagaa ggccgctttt ttgcagactc ctgaatcttt tcgattagtt tttcgattcc
      961 ttgctttttc aatacgctga ttggtaaaac tggtacacca agaccatagg aaagcttttc
     1021 tagattgatt ttttttcctt cttttttgat gacatccatc atattcagac caatgatcat
     1081 aggaatgtct aaatccaata actgcgtagt taaataaaga tttcgttcta aattggtagc
     1141 atcaataatg ttgatgatca gttcaggctg ttcctgaacc aaataatctc ttgcaatcac
     1201 ttcttctggt gtataaggag acaaagaata gatgccgggt aggtcctgaa tatgaatgtt
     1261 cggatttttt ttcaacgatc cttctttttt ttctactgtt acgcccggcc aattccctac
     1321 atattgattc gaaccagtca agagattgaa caagctagtt ttcccgctgt ttggatttcc
     1381 gactaacgcg atattcattc tttttcctcc ctttgtatca agattagttc ggcctctgac
     1441 tttcgtaacg tcagttcata ccctcgcact gtcagttcga ttggatctcc cagaggggct
     1501 agttttcgga taactatttt tacatttcgc gtcaatccca tatccattaa ccgtcgcttc
     1561 actgcgcctt gtccgtggat tgctacgacg atcccagtct ctcctgcttt taattgatca
     1621 agagaagtcc agctttcaga ggaagcagtc tttttattga caaagatttg acctagtatt
     1681 tcccgactta ctgccactcg atgacttttt accagtaaaa tggcattgtt gcctgataaa
     1741 ctgacgacga cgattttact tccggcaacg atcccgatat tttgcaaatg gcgttttgtt
     1801 ttttcttctg tttcgattcg ttcgacttga taaacgaaat tctgctttgc ctgcgctaag
     1861 gtctgcatat tgattcctct cttgtgaaat aaatcatgta ttaaaatcat tcattatatg
     1921 ttctaatttt cttacgtgtt acgcaaataa atgagaatca ttctcacata gaatactctc
     1981 ggtaattgag aatgtcaatt atacttgtga tattatgagc gaatctttag tattgataga
     2041 taaaaatgag aaaaaaatat ttcttcatag ttttccacaa ttttttctct attaaatttg
     2101 ctattttccc acacgatact tgttttcagc tcttttttca aaaacacaaa acttagtatt
     2161 ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg acttttttac taagagtttt
     2221 atactatcta ttatagataa taagaatgtg ttagaggagg cactcaccat gaacgtaaaa
     2281 atcttttcta aaaataattg tatgcaatgt aagatggtca aacggttttt aacagaaaat
     2341 caaattgctt ttgaagaagt caatatcgat gaacagcctg aagcagtaga ctggttgaaa
     2401 gcacaaggat tccaaagtgt tcctgtcatt acttctgatg cagaaacagt tatcggattc
     2461 cgtcctgatc agttgcgtaa acttgccagc taacatgact aaaagaatca aaacgtgata
     2521 catggatttg aagggcttga gacagcacca gtttttgtcg caaacttcca aatccttttt
     2581 taaagcttaa aaacaatgca atgatttgca atcaagcatc atcaaatacg aaatgaaggt
     2641 tgggcgtacg tatgagtctt aaaaatttac aagatgtcag ttatttcaag ctgaataatg
     2701 aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa ggataaagaa gctttagttg
     2761 ccttttttga ggaaaatgtc aaaccaaata cgatgacttt tccatctatt atggaaaaaa
     2821 tcaattactt gatcgatcaa gaatatatag aagcagaatt cattcaattg tattcaccat
     2881 catttattga aaagctatat caatttttgg acgagcaaca tttttctttc aaatctttta
     2941 tggctgctta caaattttat tctcaatatg cgttgaaaac caacgacggt tccgcttatc
     3001 tggaaactta tgaagatcga gtagccttca atgcgcttta ctttgcgaac ggggatgaag
     3061 aattagcact tgctcttgca gacgaaatga tccatcaacg atatcagcca gctacacctt
     3121 ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt gtcttgtttc ttgactcaag
     3181 tcactgacga tatgaactct atcgggcgct caatcaactc tgctttgcaa ttgtcccgta
     3241 ttggtggcgg cgtagggatt actttatcaa atcttcgtga agccggcgct cctatcaaag
     3301 gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa attattcgaa gatagtttca
     3361 gctattctaa tcaattaggt cagcgtcaag gagctggcgt ggtttatttg aacgttttcc
     3421 atccagacat tattgctttc ctttcagcaa aaaaagaaaa tgcagatgaa aagattcgcg
     3481 taaaaactct ttctcttggc gtgatcgtac ctgataaatt ttatgaattg acacgtaaaa
     3541 acgaagatat gtatttattt agtccatata gtgtcgaaaa agaatatggt gtcccattct
     3601 cttatgtaaa tattacagct gagtatgatc atctagttgc aaatccaaac attcgcaaac
     3661 aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa attacaacaa gaatctggct
     3721 atccttacgt aatcaatatt gatacagcga accgcgagaa tccaatcgat ggcaagatca
     3781 tcatgagtaa tttatgttca gagattttac aagttcaaac gccttctgtg attaacggca
     3841 aacaggaata tgaagtatta ggaacagata tctcatgtaa cttaggctcg acaaatatcg
     3901 tgaatttgat gcaaagccct aactttggtc aatctgtgcg tacgatgact cgtgcattga
     3961 catttattac agatgcatct gatatcgatg tcgtaccacc gatccaaaac ggaaataagc
     4021 tgaatcatac gattggatta ggtgccatgg gcttacacac ttatcttgca aaagaacaaa
     4081 tggaatatgg ctcagaggat tcattagact ttacaaatat ttacttcatg ctgttgaatt
     4141 attggacact tgttgaaagc aacaatatcg ctcgtgaaag aaaacaagtt ttccataatt
     4201 ttgaaaaatc agcttatgct gacggcactt atttcgaaaa atacgtaact ggagaattcc
     4261 agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat ttttatccca actgctgaag
     4321 attggaatgc tttgaaacaa gcagtaatga aagatggact gtatcatcaa aatcgcttgg
     4381 cagtagcacc aaatggctca atttcatata tcaacgatac gagtgcaagt attcatccta
     4441 tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg taaaatttat tacccagcgc
     4501 cttatctatc caatgagacg attccgtatt acacttctgc ttatgatatg gacatgcgta
     4561 aagtaatcga tgtttatgca acggcacaac aacacgtcga tcaaggcatg agtttgactt
     4621 tattcatgcg ttctgaaatc ccagaaggca tttatgaatg gaagacaaca acgaagcaaa
     4681 cgacacgtga tttgaatatt ttaagacatt acgctttcca caaaggaatc aaatccattt
     4741 attacgtacg tacctttact gatgatgcag aagaaatcgg aagcaatcaa tgtgaaagct
     4801 gcgtgatcta atatcataga tagattcgct tattcatatt ttaattaaag gagcattctc
     4861 atgtcagaaa catattatga agcaataaac tggaatgaaa ttgaagatat cattgataaa
     4921 tccacttggg agaagctgac agagcaattt tggctagata cacgtatccc actttcaaat
     4981 gacctagatg attggcgtac attgtctcaa ttggaaaaag atacagtagg acatgtattt
     5041 ggcggtttga cactgctaga tactgtccaa tctgaaagtg gcatggatca gttgcgtaaa
     5101 gatgtccgca ctccccatga agaagccgtt ttgaacaata ttcaattcat ggaatccgtt
     5161 catgccaaaa gttattcttc tatttttagt acactgaata cgaaaaaaga aatcgaagaa
     5221 atttttgact ggacaaatac gaatccttat ctgcaaaaaa aagctgagcg tatcaatgaa
     5281 atctacaaac acggtacacc gctagaaaag aaaattgcca gcgtcttttt agaaacattc
     5341 ttgttctatt caggttttta tactcctctt tattatttag gaaacaacaa attggctaac
     5401 gtagcagaga tcatcaaact aatcatccga gatgaatctg tacatggaac ctacatcggt
     5461 tacaaattcc aattaggatt caacgagtta cctgaagcag aacaagaaac gttaaaagac
     5521 tggatgtaca atcttcttta tgagctttac gaaaacgaag aacgttatac agaagaatta
     5581 tacgatccaa tcggctggac agaagaagtc aagacattcc tacgctataa tgccaacaaa
     5641 gcattgatga acctagggat ggatccactc tttccagata ctgccaatga tgtcaaccca
     5701 atcgtaatga acgggatttc aacaggcaca agcaaccatg acttcttctc acaagtcgga
     5761 aacggctatt tgttaggtca tgtagaagcg atgaaagacg acgattattt gattggattg
     5821 gaataaggat ttattttaat tcaaaaattt ttcgaaaaat aaaaagagtt tagcgtacag
     5881 atattttaaa tgtacactga actctttttt atgcttattc acttgtttct tccagcactt
     5941 ttggactgtc taacttttcc gaaacatgtt ccattcttct caataaatag attttccgga
     6001 tatttaatat tatcgtctta atcactgcat agacaggtat gcaaatcagc atgcctaata
     6061 ttcctgcgac acttcctgct ccaatcaata ccaagatgat cgtcaaagga tggatattca
     6121 ttgatttccc aaataacaac ggcttgatca aatttccatc taattgttgg atcactaaaa
     6181 tcgacaacgc catatacaaa gcttgaaatg gagagataaa caaaccgaca atgattgctg
     6241 gtaccgctcc aataaaaggg ccgacatacg ggataatatt cgtgattccg caaatgaccg
     6301 caagcaacaa gctataaggc tgatcgaata ttctcattgc caaaaagctc attacaccaa
     6361 taatcaaagc atctaagatc gtactgctga tataggcaga taatgtttcg tttaattctt
     6421 ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa gaagtgagac aacgcatcaa
     6481 taaatttgtg cccatccttg aacataaata gcaaaataaa tggaacggta aataacaaaa
     6541 taaaaaattg catcataaat gaaatgattt ttgagacact tgcagataaa ctgactaata
     6601 tgatattgaa caaattacta agagataaat ttgctgatgc taattgttct tccaaattga
     6661 aatttttaaa ctcttcccgc tgcgccaatt catttaacca tttgcttgtt tcatctgcat
     6721 acattggcaa gctctgggtc aattgaattg tctgttccat taactgaggc actacattca
     6781 tgacagttaa aacaactaaa atgattacga ccaaaaacgt caataaaaaa ccgaacactc
     6841 tcgggatccg atgcttttcg agaaaaacaa cgattggatc gaacatataa tagataaaac
     6901 cagcaacaag caatggtaaa aaaattgccg aaagcatttt caatattgga ctaaaaatat
     6961 tcggcatttg taaaataaag tacacgccga tgatcgtgag tactagctcc actgtccaga
     7021 aaaataattt tgattgttta aaacgtgatt gcatacatct cctccaaggt cctttgatac
     7081 ttctattcta tcatttaaca aagagttacg cgctgagatt ttcatttgct taaaaaaaac
     7141 aaaaaaaata aacatatttt aatgagtaga cgagtactta aacaaaaaca cggtttggag
     7201 tagtgacaaa aattatactg cagtcccttt ttccaaataa acggtgttca aaagcttgca
     7261 tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac tatttttatt tggctgggct
     7321 tattacttga tgtaggacat ctattttaca acctcagatc atttattgcg caatactgac
     7381 taaatgcaac tttgtgatat ttttgtgtac ggacaactcc tcgatcacat cggcataagt
     7441 gagccctttt ggaagatcaa tcgtatagat atttttgtac actcggtagt catcaacgaa
     7501 ttttacgtca aaattcacat cttcgattac gatatttttc tcttcaaaat aatcggtaag
     7561 aaaatctttg gtttcttttc tatggataaa ttggatttct aactttttag tcgtaggtac
     7621 atggatcacg cgtttcacta ctgtgagtgc aatagcaatc ccgataaaac tgatgatcgc
     7681 aatggaatag taacccatgc cgatggcgat ccctaatcca gcaattgccc ataaagaagc
     7741 ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg atcgttcccg cgcctaagaa
     7801 accgacacca ctcaccactt gagcaatcag ccttgcttca tctgaacgaa tcgttccagc
     7861 aagctgtgga tactctaaag catctcttaa tgcaccacta gcgatctcaa cttggatcaa
     7921 tgcaatgata catgctccca tacaaactaa aatatgcgta cgcatacctg ctggacgatt
     7981 tttatattgt cgttcaaaac cgattgctcc gccaaatagc atagcgatcc ctaaacgtaa
     8041 aataatctct ggaattgtca ataccatatc attcatctaa acaccctcca gtccaatata
     8101 tcatgtctga aaggagcaga caaaaaaagt gcaattttta cctcttacga aaaaaggact
     8161 gtgacaaaat ttttgtcata gtccttttta tgaataaacg gtgttcaatc agctgaccct
     8221 cggtattact caacaaacgg caaaatatgg ggagctattt ttcctttgtc ttctctaata
     8281 ctcaggtcaa aacgctgatt tcacaacctc tttagcctac tattgaaaat tacaacttac
     8341 ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg attttgcaat acacttgctg
     8401 gcaaatgatt cgttacaggt ccttcaatca tccctttgat tgcatcagct ttgctttcgc
     8461 caaatgccat caagacgatt tctttacttt tcataattga accgattccc attgaaacag
     8521 cttttgttgg tacatcttct gctttatcga agaatcgttt gtttgcattg attgttgatt
     8581 ctgttaagtc aactactgaa gtcgttccgt caaatgaagc accaggttca ttgaaaccga
     8641 tatgtccatt ttgcccgatt cctaagattt ggatatcgat tggatgtgca tcgatgattt
     8701 gatcatactt tttgcatgct gcttccagat cttctgcttt tccatctggt acaaacgttt
     8761 ctttgaatgg ttttttatca aataattgga catccatgaa atgacggtag ctttgttcat
     8821 ctgaaccgcc tagtccaacg tactcatcta agtttacaga agtcatttca ctgaaatcca
     8881 aatcgcttga agtcatttct ttatataaag taactggtgt gcttcctgtt gccaatccta
     8941 atacttttgc acctttgttc ataccttctt tgatgatttc aaatgctttt tttccgcctt
     9001 cttcagcgtt ttttacacga atgatttcca tagtgattgc tcctttatat tttggtatag
     9061 cccaattata gcattctttt tttcttttta caataattag tatagaccaa attgcatttt
     9121 tttcattttc ttgacaataa ataggaatcc gtgttaggtt tcataagatg gttcaatcaa
     9181 acaatccatg ttgcatgaaa accaagcaag tgattcactg tggacgcttt atgtctgcag
     9241 tttttttatg aaaaaggagt tattatgagt atactgacat taaacaacat tacccagcaa
     9301 tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat taaatgctgg cgaacatctt
     9361 gggctgattg gacaaaacgg agccggtaaa agtacattga ttaaaatcat cactggtgag
     9421 atcttaccag atgacggaca gatccattgg caaaaaaata ttcacaaagg ttacctcgat
     9481 caatatgtgg aagtcgatga aacattaaca atcgaagaat ttctaaaaac tgcgtacgca
     9541 aaagaatacg aaaaggaaga aaaaattgct tttctttacc aaacatatag cgagaccttc
     9601 gatgattcct tgctggaaaa agctgggaaa ctccaaacgg aattagacca aggagtgttt
     9661 tatcaaatcg acaccttagt tgctgagatg agcagcgggt tggggatcga tgtcttaggt
     9721 ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt caaaggtgat tttagcaaaa
     9781 ctacttttag aaaacccaga tgttctgatc ttagatgaac caaccaatca cttagatgat
     9841 cagcatatcc aatggctgac gcagtttctt caggattttg acggtgcgta tatcgtgatt
     9901 tcccatgacc aagaatttct tgatcagatc accacccata tcgcagacat cgagtttgga
     9961 aagattacta agtatactgg ccatctaaaa caagcattga aacaaaagga acaaaatcgc
    10021 gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa tcgaaaagac cgaagcttat
    10081 attcgaaaat acaaagccgg ttcccgatca acgatggcaa aaagccgaca aaaacaatta
    10141 gataaaatcg aacggctgac gcctccagct tcagcaagta aagctgtctt tgattttcca
    10201 ttctcgccta ttgtcacgac attagcagtg gaaacaactg atttagtcat cggatatgat
    10261 cgaccgctac ttgcacccat caatttgacc atccgctttg gtgaaaatat tgccattcga
    10321 ggcttcaacg ggattggaaa atctacttta ttaaaaacac tgatcggtga gattgaaaaa
    10381 ataagtggcg atttccactt cccagataac acaaaaatca attacttttc acaagatctg
    10441 tactgggaaa atccattaga aacaccacta caatatttaa gcagtcaatt tccaaaagcg
    10501 actatcaaag aattacgtcg ccaactagct aaagcagggt tagttaatca attagcaagt
    10561 gaacctctca ctacattaag tggtggggaa caaactaagg tgaaacttgc acagttgaca
    10621 atgaaccaag gtaatttact gattctcgat gaaccgacga accatatcga tcaagaaacg
    10681 aaagaaagct tacaagacgg gatacagaaa tttccaggaa cagtaatcat cgtctctcat
    10741 gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg agattgaacc aaaataaacg
    10801 tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt tttgtcacaa ctccttcttc
    10861 tgaataaatg gtggccaatt agctgacctt cggtattagc tcaaaaaacg gcaaaatatg
    10921 aggagctatt tttatttggc tattcttatt acttgatgca gaacagcttt tcacaacctc
    10981 ttttcgtcta ttttttattt tcgagatttc tctttccttt aaaaacaact gtttgaaaga
    11041 agaacgcaca tccttgaaaa acaagcccgc taaccgttac acctaaccag ccatactgct
    11101 gccacattgt cgtccctagc aatgaaccag ccgcacctcc aataaagtac ataaacataa
    11161 ataca
//
LOCUS       DAZUMV010000092        10666 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000092 DAZUMV010000000
VERSION     DAZUMV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.093"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1232)
                     /locus_tag="ACG8FK_002028"
                     /pseudo
     CDS             complement(15..1232)
                     /locus_tag="ACG8FK_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            1723..2661
                     /locus_tag="ACG8FK_002029"
     CDS             1723..2661
                     /locus_tag="ACG8FK_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5097622.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2791..3438
                     /locus_tag="ACG8FK_002030"
     CDS             2791..3438
                     /locus_tag="ACG8FK_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFS5097623.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(4131..5069)
                     /locus_tag="ACG8FK_002031"
                     /pseudo
     CDS             complement(4131..5069)
                     /locus_tag="ACG8FK_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(5069..5686)
                     /locus_tag="ACG8FK_002032"
     CDS             complement(5069..5686)
                     /locus_tag="ACG8FK_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5097624.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(5700..5870)
                     /locus_tag="ACG8FK_002033"
     CDS             complement(5700..5870)
                     /locus_tag="ACG8FK_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097625.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(6218..7709)
                     /locus_tag="ACG8FK_002034"
                     /pseudo
     CDS             complement(6218..7709)
                     /locus_tag="ACG8FK_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
     gene            complement(7752..8051)
                     /locus_tag="ACG8FK_002035"
     CDS             complement(7752..8051)
                     /locus_tag="ACG8FK_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097626.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8417..8701)
                     /locus_tag="ACG8FK_002036"
     CDS             complement(8417..8701)
                     /locus_tag="ACG8FK_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5097627.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(9220..9705)
                     /gene="vanZ-A"
                     /locus_tag="ACG8FK_002037"
     CDS             complement(9220..9705)
                     /gene="vanZ-A"
                     /locus_tag="ACG8FK_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFS5097628.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(9858..>10666)
                     /gene="vanY"
                     /locus_tag="ACG8FK_002038"
     CDS             complement(9858..>10666)
                     /gene="vanY"
                     /locus_tag="ACG8FK_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS5097629.1"
                     /translation="YDQNPKEHLENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQ
                     ESVKSDIVNLSKHDELINGYGLLDSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGY
                     RDFDEQSVLYQEMGAEYALPAGYSEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYG
                     FILRYPEDKTELTGIQYEPWHIRYVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVN
                     GEKYEIFYYPVTKNTTIHVPTNLRYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3280 a         1849 c         1647 g         3890 t
ORIGIN      
        1 aagggctttt atgtttattt tgaaaaagag ataaaatcaa tatatccctt ttccccaatt
       61 tttacaacgg cattgtaggg ctttttctct ttgtttttga ttccttttac cagggtttct
      121 tttccctcca gtaattcttt tacattcgtt ttggttagtt ttttctttct aaaatgttca
      181 gctaaagtaa acttacattc aggataattt gaacaaccat aaaacgattt ttttaataca
      241 atattgttgc cacacttagg acattttcct acaatacttt tttctgcttc tttttctttc
      301 tgttcctggt aatcagaaaa atttagtttt tctatatcgt taggtacagc ttccagtaaa
      361 tgaacaatga attttttgat attcgtaata aagttctctt gattgccttc tcttttaccg
      421 atttttttta aatacgtttc ccatttagcc gtcatttcag cactcgttaa aaggtgctga
      481 ctttcaactg cctggcacaa taattttcct ttttcagtta caacaagctt attcttaatc
      541 acttggatat attctttttg ttttaacgct tcaataatac ttgctcttgt cgcttctgtt
      601 ccaatcccct caacttcttg taaaatcttg attgcttctt catcatcaac cgttttattc
      661 gccgttttca tagcagttaa taatgtaccc tctgtaaaag cttttggcgg ttgcgtttct
      721 ttttcaacac tcttaacacc aacttcggca cgttcgccaa tgataacgag tggtaacgtt
      781 tgaacgtcct ctttttcttc tttggtttgt tgtttgaaaa gaattttcca cccttcttgc
      841 tttggtgtct ttccgatcga ttgaaacagc aaatcagcta ctttcgtttg tatcttggtt
      901 tcttcataca agtaatcagg taaaaacatg gcaacggtcg ttttaacgac taaagcataa
      961 atttttcgtt gtaaatcgtc cattttcgct aatgcagatt cggtaggtac ttgttttgtt
     1021 gggataatgg cgtggtgttc ctgtacctta ctaccgtcca cataacgctt tctaggctct
     1081 gtttgaacca tttcaagatc aagtcctaaa aaaccgctat atttgccaaa attcgctttt
     1141 aaataagcaa attcgttctc tgtaataaat ggtgtatctg ttcttggata actcaataat
     1201 tttgcttcat acagtccttg catagctttt aaacgtaaaa ttttgcgttc taagacgttt
     1261 taaatatatc tgcgtatatt tttatgtgtt ttatcttttc gtcggcaaat gcgttaaaat
     1321 ttgcgaaata aaatagctca agggcgaacg cagtgagtgc atttaccctt gatctatttt
     1381 tttgagtaaa ttagcttttg aaaccgacgg aaaggaaaca caaaactttt cagaggcaag
     1441 gggtttttaa ggggcgcttt tagccccttg aatttatttg ctttaagata tatttagttt
     1501 taaaatagtt ttaacgaaaa aaagacataa aaaaagacga gcaacgctac caacgttctc
     1561 gcccggaata acaaaccaca cttgttattc attcgatgat tagtatatca atttttgatt
     1621 ttgaatacaa aacaaaatgg acgaatggat acaggcgaaa tttattttgc gattccactc
     1681 gtcctttttt cgttccaaaa attattttag gagcgataaa aaatgaagaa aattgatgaa
     1741 aaattagctg cagaaatctc actaaaaaaa ccggccgtct ctaatagccg gttaagtaat
     1801 agccggttaa gtggtcaaac tttgggaaaa tctcaacccg cattaagttt tgatgccatg
     1861 acaatcgttg gaaatttgaa caaaactaat gctaaaaagc tatctgactt tatgagtgta
     1921 gagccacaaa tacgactttg ggatatactt caaacaaagt ttaaagctaa ggcacttcaa
     1981 gaaaaagttt atatcgaata tgacaaagta aaagcagata cttgggatag acgtaatatg
     2041 cgtgttgaat ttaatcccaa taaactcaca catgaagaaa tgatttggtt aaaacaaaat
     2101 attatcgact acatggaaga tgacggtttt acaagattag acttagcttt tgattttgaa
     2161 gatgatttga gcgattacta tgcaatgact gataaagcag ttaagaaaac tgttttttat
     2221 ggtcgtaatg gcaagccaga aacaaaatat tttggtgtcc gtgatagtga tagatttatt
     2281 agaatttata ataaaaaaca agaacgtaaa gataacgcag atgttgaagt tgtgtctgaa
     2341 catttatggc gtgtagaagt tgaattaaaa agagatatgg ttgattactg gaatgattgt
     2401 tttaatgatt tacacatctt gaaacctgcg tgggctactt tagaaaaaat taatgagcaa
     2461 gctatggttt atactttgtt gcatgaagaa agtatgtggg gaaagctaag taagaatact
     2521 aagactaaat ttaaaaaatt gattagagaa atatctccaa ttgatttaac ggaattaatg
     2581 aaatcgactt taaaagcgaa cgaaaaacaa ttgcaaaagc agattgattt ttggcaacgt
     2641 gaatttaggt tttggaagta aaataagttt tatttgataa aaattgctaa ttcagtataa
     2701 ttaatattta cgaggtgaca taacgtatga aaaaatcaga ggattattcc tcctaaatat
     2761 aaaaatttaa aatttaggag gaagttatat atgactttta atattattga attagaaaat
     2821 tgggatagaa aagaatattt tgaacactat tttaatcagc aaactactta tagcattact
     2881 aaagaaattg atattacttt gtttaaagat atgataaaaa agaaaggata tgaaatttat
     2941 ccctctttaa tttatgcaat tatggaagtt gtaaataaaa ataaagtgtt tagaacagga
     3001 attaatagtg agaataaatt aggttattgg gataagttaa atcctttgta tacagttttt
     3061 aataagcaaa ctgaaaaatt tactaacatt tggactgaat ctgataaaaa cttcatttct
     3121 ttttataata attataaaaa tgacttgctt gaatataaag ataaagaaga aatgtttcct
     3181 aaaaaaccga tacctgaaaa caccataccg atttcaatga ttccttggat tgattttagt
     3241 tcatttaatt taaatattgg taacaatagc agctttttat tgcctattat tacgataggt
     3301 aaattttata gtgagaataa taaaatttat ataccagttg ctctgcaact tcatcattct
     3361 gtatgtgatg gttaccatgc ttcactattt atgaatgaat ttcaagatat aattcatagg
     3421 gtagatgatt ggatttagtt tttagatttt gaaagtgaat ttaattttat acacgtaagt
     3481 gatcataaaa tttatgaacg tataacaacc acattttttg gttgcttgtg gttttgattt
     3541 tgaatttggt tttgaactta tggactgatt tattcagtcc attttttgtg cttgcacaaa
     3601 aactagcctc gcagagcaca cgcattaatg acttatgaaa cgtagtaaat aagtctagtg
     3661 tgttatactt tacttggaag atgcaccgaa taaaaaatat tgaagaacaa ctagcaaaag
     3721 attttaaaga gttattttat tttaagtctt tataacatga gtgaagcgaa tttttaaatt
     3781 tcgatagaaa tttttacatc aaaaagcccc ctgtcaaaat tgacgaaggg ggttttttgg
     3841 cgcacgcttt tcgttagaaa tatacaagat tgaaaatcgt gtataagtgc gccctttgtt
     3901 ttgaacttag cacgttacat caatttttta aaatgatgta taagtgcgcc cttttaaatt
     3961 ttgagtgatt atattttttg agttagaaaa agggattggg aaaatttccc aaaataattt
     4021 aaaaaataag caaaaatttt cgatagagaa tgtgctattt tttgtcaaag gtgtatacct
     4081 tgactgtgct tgctgttaca ttaagtttat ttttaagtta ttaaaaaaga aatagctttt
     4141 aaagtttggc tcgctgtcgc tttataaagc tgattgactt ttgattgcaa actacttaaa
     4201 gaaaacaaac tcggactatt cgttttcttc tctttggttt gaacatcagc aattatcccc
     4261 tcttgattgc ctattttagc ttgtttagaa gaaacaaaag ctaaaagctc ctcttgggtt
     4321 ttaaaacgct gtgtggggct tagaacgccc ttaaacgacc cttggtttac ttttatacta
     4381 gcttccacct cgaaaaaagg ttctttttta aaattctcta tggcttcctg gcgctgaaaa
     4441 ataaggtata aggtgggcgt ttgaacacgt cctagtgaaa atgtaccttg tacgcccttc
     4501 tgttgtaaat ttaacgtata caaagggctt gcgttcatgc cgatcaacca atcggcaatt
     4561 tggcgtgttt gcgcttcttg ataaaaggga tagtaattca ttccaggttg caaattttga
     4621 aaaccgcttc ggattacatc tttttctaag ctattgatcc atagtctttt aaatgtttta
     4681 tcttttgaaa aggcatttgc tttatggata atcgaccagg cgatattttc accttctctg
     4741 tcgctatctg ttgcaacaat aattgtattt gcctttttga gaagttctgc aacaatttta
     4801 aactgctttc ccttatcttt tgcaacttca aaatcgtatc gatcaggaaa aatcggcaaa
     4861 gattcaagtt tccaattttg ccacttttcg tcataatgac ctggttctgc taattccact
     4921 aaatgcccaa aaccaaaggt gataaacgtt tcatctgtaa atagtgggtc tttgatctca
     4981 aaataaccgt cttttttggt gctttgtttt aaagcacttg cgtaggctaa tgcctggctt
     5041 ggtttttcag ctaaaataac cgtactcatt aactatccct cttttcattg ttttttcttt
     5101 gatcgactgt cacgttatat cttgctcgat accttctaaa cgttcggcga ttgattccag
     5161 tttgttcttc aacttcttta tcggataaac cattcaaaaa caaatcgaaa gcatgcttta
     5221 aacgtggatc attttcttta aatttatgct gacgaccttt gaatttgcct tttttcttag
     5281 caatttcgat tccttgtgcc tgacgttcct taattttttt tcgttctgat tctgcttgat
     5341 acttgtacaa ttcaatgaca aggctattaa tcaaacgcct taaattttca tcttcaatac
     5401 cattcattga gggtaaattt aagacttcca gggttgcccc cttaatttga atttgattca
     5461 tcaattctgt taattcttta ttatttcgtc ctaatcgatc taattcagta acaataacaa
     5521 tatccccttc acgaatatag ttaagcatag cttgtaattg tgggcgttcg accgattgac
     5581 cgcttaattt gtctgaaaag accttagaaa cgccctgtaa cgcttgtaat tgccgatcta
     5641 agttctgttc tttgctactg acacgtgcat aaccaatttt agccattttc aaccaacctc
     5701 taaaattctc tcggttgcaa taaccaatca gcaatatcta ctttttcaat ttcaaattgc
     5761 ttatcagaaa ttgtcttttc gtaagcgata aaatcttgcg catattgttg ctcattaaaa
     5821 atagccacca cttcgtcatt ttctaaaact cgataaataa attttttcat tttactcctc
     5881 ctattatgcc caacttaaat gacctattca ccaagtcaat tatactgcta aaatcatatt
     5941 aggacaaata ggtatactct aatgacctat aaaagataga aaattaaaaa atcaagtgtt
     6001 cgcttcgctc tcactgcccc tcgacgtttt agtagccttt ccctcacttc gttcagtcca
     6061 agccaactaa aagttttcgg gctactctct ccttctcccc ctaataatta attaaaatct
     6121 tactctgtat atttctgcta atcattcgct aaacagcaaa gaaaaaacaa acacgtatca
     6181 tagatataaa tgtaatggca tagtgcgggt tttattttca gcctgtatca tagctaaaca
     6241 aatcgagttg tgtgtccgtt ttagggcgtt ctgctagctt gtttaaagtc tcttgaatgg
     6301 aatgtatgct ctaagtcaaa agaatttgtc agcgccttta tatagctttc tttttcttct
     6361 ttttttactt taatgatcga tagcaacaat gatttaacac tagcaagttg aatgccacca
     6421 tttcttcctg gtttaatctt aaagaaaatt tcctgattcg ccttcagtac cttcagcaat
     6481 ttatctaatg tccgttcagg aatgcctagc acttctctaa tctctttttt ggtcgtcact
     6541 aaataaggct tgtatacatc gcttttttcg ctaatataag ccattaaatc ttctttccat
     6601 tctgacaaat gaacacgttg acgttcgctt ctttttttct tgaatttaaa ccacccttga
     6661 cggacaaata aatctttact ggttaaatca cttgataccc aagctttgca aagaatggta
     6721 atgtattccc tattagcccc ttgatagttt tctgaatagg cacttctaac aattttgatt
     6781 acttcttttt cttctaaggg ttgatctaat cgattattaa actcaaacat attatattcg
     6841 cacgtttcga ttgaatagcc tgaactaaag taggctaaag agagggtaaa catgacgtta
     6901 ttacgcccta ttaaaccctt ttctcctgaa aatttcgttt cgtgcaataa gagattaaac
     6961 cagggttcat ctacttgttt tttgccttct gtaccgctta aaaccgttag acttgaacga
     7021 gtaaagccct tattatctgt ttgtttgaaa gaccaatctt gccattcttt gaaagaataa
     7081 cggtaattag gatcaaaaaa ttctacattg tccgttcttg gtatgcgagc aataccaaaa
     7141 tgattacacg ttagatcaac tggcaaagac tttccaaaat attctcggat attttgcgaa
     7201 attattttgg ctgctttgac agatttaaat tctgattttg aagtcacata gactggcgtt
     7261 tctaaaacaa aatatgcttg ataaccttta tcagatttga taatcatagt aggcataaaa
     7321 cctaaatcaa tagcggttgt taaaatatcg cttgctgaaa tagtttcttt tgccgtgtga
     7381 atatcaaaat caataaagaa ggtattgatt tgtcttaaat tgttttcaga atgtcctttc
     7441 gtgtatgaac ggttttcgtc tgcatacgtt ccataacgat aaacgttggg tgtccaatgt
     7501 gtaaatgtat cttgattttc ttgaatcgct tcctcggaag tcagaacaac accacgaccg
     7561 ccaatcatgc ttgattttga gcgatacgca aaaatagccc ctttgctttt acctggcttg
     7621 gtagtgattg agcgaatttt actattttta aatttgtact ttaacaagcc gtcatgaagc
     7681 acagtttcta caacaaaagg gatattcatt cagctgttct cctttcctat aaatcctata
     7741 aaataggttg tttaattaac ttggtttgct ttttcattca actgtttcaa tattgcatgt
     7801 tttgaaaaag atttttttcc tttataagtc aatttttttc cactaatcga ataaattatt
     7861 ttgttatttt ctattaactt atatatataa tcttccccct ccgaagaaaa atacttatct
     7921 gattttgttt ctaagtagat atttctcttt tctaactctt tcttaaacgt ttctagtgta
     7981 tagatatttg ctaattttct tatctccaat aaactatttt ttatataagt tttacattca
     8041 tcatgattca tacaaactcc accttctata aatgaataca aaaaaagcaa tcaaacgatt
     8101 tccgattgat tgcttaacaa ttcttaaatt cagtagctta gatacttgaa aactctctga
     8161 tttccctata taatgatagt acggttatat accgtcttca aacaaagtta attaaataac
     8221 ttcttacgag ggaagagttc atctgactaa ctgataagcg ttggtttggc aatcttatcg
     8281 ggctatgcat ttataaaatg tcgtcaaaca ttttataaat gtgtcatggc tcttttttcg
     8341 tttctattca gttcgttgtt tcgttatatc tagtataccg cttttaaaaa aaataagcaa
     8401 cgatttcgtg cattattcac acgaagtcat tgcttttttc ttcttccatt tctaaatcca
     8461 atgttacttg ttctgattct gtttctggtt ctggttctgt tggctcattt gggattaaat
     8521 ccactactag cgttgagtta gttaactttg caatttgttc tagtgttttt atggttggat
     8581 ctgattttcc tgattctatt cgtgaataat ttgatctact catttctaat tcttgggaga
     8641 atttttcttt tgatttggta cctctcatct tttttaagct ttctctaaat gctagttcca
     8701 agtttctccc ccctcttttt gtgtaataaa attcacgctt tatttaaatt atagctttta
     8761 atatcttaat aaacaagtga ttaatcactt gtgatttgtg attattccaa aagtaggtaa
     8821 tttcaccaac ctttttcaaa agtttttatc gacgattatt ggactggatc gtgtttccta
     8881 aagcgattct aagcgcatgt ttaattttaa agtgggtaat tctctttaaa acgtcctaca
     8941 aagattcaaa aaaagcatgg ctgctagtgg gggtaccgtc aggaaaatgc ggatttacaa
     9001 cgttaaggaa atttattaaa gagaccaatt cctcagcact aaaattgata attgggcttt
     9061 ctttgttact atagtgttag gtaagctttt tactttatca ccggttttcc cctcacttca
     9121 cacctacggg cgagtttccc cacatcccgt tttccatcta atccaattca ttcaatatct
     9181 ttatgtgtat gaaagatcag attgcttgat ttagacatct tacacgtaat ttattcttaa
     9241 atgggtacgg taaacgagca ataatacgag caaaagtata cctacaaaaa taataactct
     9301 gtctaatttt ttttgattca tatgcttatt gcttaaacca tataatttca gtccaagaaa
     9361 gcctccaaca gtatttgtaa ttacatctgt tatgtctgtc gctccaatag cgaagataaa
     9421 ttgaattatt tcaaaagtaa gacttaaaac cagtacaaaa gcaaacttag gtaaaaatcc
     9481 gatttcttta aaattgacat tcaaaagcaa gccaaatgga ataaagatta taacattatc
     9541 tatcatctct ctgaaattcc cagtagcagt aaatggagtc aagttaagac ttctttgatg
     9601 ataattaaat actgataaaa tattgtattg taatttgaat aacactaacc agattagtgt
     9661 cactaaatat aaagctagca atcctctaga taatattttt cccaatataa tttcctctaa
     9721 ttcttttgtt taagttgtat cattagtata gcaatgtcct agttaacgtg ggttttaaat
     9781 taaaaagcta acgttacccc aaaagtgcta gtacaataat gctgttcatt aatttcgttg
     9841 gtttcattcc gccatcctta cctccttgaa ttagtatgtg ttgatccggg aaacactgtc
     9901 acaattacac cgtctatatt gtttcctgat atctcataac gaagattagt cggcacatga
     9961 atggtggtat ttttagtaac aggataataa aagatctcat atttttcccc atttacacta
    10021 acagaaatgg ttttttcttc ttttaggtaa tccatatatt cctcgagaac gaaattcttt
    10081 tctttcataa tcgcactatg tggtaaacca acatagcgaa tatgccatgg ttcatattga
    10141 attcctgtta actctgtttt gtcctctgga taacgtaaaa tgaacccgta tttccaagca
    10201 ttttcttcta tccactttcc ttcaggggct cgttccattt tcgtcaagct tgatcctaca
    10261 tctagtgata aacctgaatt atgctcacta taacctgctg gtaaggcata ctcagccccc
    10321 atttcttggt aaagcacact ttgctcatca aagtctcgat agccactatt aataataaaa
    10381 tgactaacgc caccctttac agcatcattg accatctctg aaaatttttg tgctatttct
    10441 tttgacatat aaatattact atcaagcaac ccgtatccat ttattaattc gtcatgttta
    10501 gataaattca cgatatctga cttcacactt tcttggcgaa caggatattt actattgatt
    10561 aatagcagat ttccttgata aacctgttct tctgtaattg ttttctcttg ggtattttca
    10621 gaagtcccac tattttctaa atgttctttg ggattttgat cataat
//
LOCUS       DAZUMV010000093        10534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000093 DAZUMV010000000
VERSION     DAZUMV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.094"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..401
                     /gene="rpsN"
                     /locus_tag="ACG8FK_002039"
     CDS             132..401
                     /gene="rpsN"
                     /locus_tag="ACG8FK_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5097630.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            416..595
                     /gene="rpmF"
                     /locus_tag="ACG8FK_002040"
     CDS             416..595
                     /gene="rpmF"
                     /locus_tag="ACG8FK_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5097631.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            627..1004
                     /locus_tag="ACG8FK_002041"
     CDS             627..1004
                     /locus_tag="ACG8FK_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097632.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1025..1174
                     /gene="rpmG"
                     /locus_tag="ACG8FK_002042"
     CDS             1025..1174
                     /gene="rpmG"
                     /locus_tag="ACG8FK_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5097633.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1198..2181
                     /locus_tag="ACG8FK_002043"
     CDS             1198..2181
                     /locus_tag="ACG8FK_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5097634.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2211..2333
                     /locus_tag="ACG8FK_002044"
     CDS             2211..2333
                     /locus_tag="ACG8FK_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5097635.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2351..3874
                     /locus_tag="ACG8FK_002045"
     CDS             2351..3874
                     /locus_tag="ACG8FK_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5097636.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3898..4137
                     /locus_tag="ACG8FK_002046"
     CDS             3898..4137
                     /locus_tag="ACG8FK_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5097637.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4330..4791)
                     /locus_tag="ACG8FK_002047"
     CDS             complement(4330..4791)
                     /locus_tag="ACG8FK_002047"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5097638.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(4986..5204)
                     /locus_tag="ACG8FK_002048"
     CDS             complement(4986..5204)
                     /locus_tag="ACG8FK_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5097639.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5322..6938
                     /locus_tag="ACG8FK_002049"
     CDS             5322..6938
                     /locus_tag="ACG8FK_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5097640.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7179..8519)
                     /locus_tag="ACG8FK_002050"
                     /pseudo
     CDS             complement(7179..8519)
                     /locus_tag="ACG8FK_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8795..8950)
                     /locus_tag="ACG8FK_002051"
     CDS             complement(8795..8950)
                     /locus_tag="ACG8FK_002051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFS5097641.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(8957..9103)
                     /locus_tag="ACG8FK_002052"
     CDS             complement(8957..9103)
                     /locus_tag="ACG8FK_002052"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097642.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHIKKEIVVRP
                     LQIY"
     gene            complement(9142..9336)
                     /locus_tag="ACG8FK_002053"
     CDS             complement(9142..9336)
                     /locus_tag="ACG8FK_002053"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5097643.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9246..9578)
                     /locus_tag="ACG8FK_002054"
     CDS             complement(9246..9578)
                     /locus_tag="ACG8FK_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5097644.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10092..10283)
                     /locus_tag="ACG8FK_002055"
     CDS             complement(10092..10283)
                     /locus_tag="ACG8FK_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5097645.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10377..>10534)
                     /locus_tag="ACG8FK_002056"
     CDS             complement(10377..>10534)
                     /locus_tag="ACG8FK_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5097646.1"
                     /translation="PLLKEPEQIFLIEKFIFDKTDKEIGKVLGITRQGATNLKHRLYK
                     KIQKMLV"
BASE COUNT         3497 a         1677 c         2059 g         3301 t
ORIGIN      
        1 tattcatatg agttttattt catgttattt attgttgaca agagcaactc ttgttggtaa
       61 agtatcagtg tcaaaacgta acgattacga tttgaggtgt ctaaatcaaa ataaaaggag
      121 tataaaggta tatggcaaaa aagtcgaaaa ttgctaaagc aaagaaacaa atggcgatga
      181 ttgaaaagta tgcagataaa cgtcaggaat taaaagccgc aggagatcgc accgctctcg
      241 ctaaattgcc aagagattca aatccgaatc gtctgaggct tcgtgatcag acggatggtc
      301 gtccaagagg gtatatgcga aagttcggta tgtcccgtat taagtttaga gaacttgccc
      361 atcaagggtt gattcctgga gtaaaaaaag caagttggta aaaggaggaa atatcatggc
      421 agtaccagca agaaaaacat ctaaggcgaa gaaacggtta agaagaactc atcaaactgt
      481 agtaaaacca gaaatttcat ttgacgaagc gaatggtgat tatagacgta gccatcatgt
      541 atctttaaaa gggtactata atggaaagaa agtaattaaa ggagcttcaa aatagtttaa
      601 tatataactt ttaggaggga atcaaaatgg aaattattta cccaccttta gttgaagaag
      661 gattaaagta ttatcttgaa acgacccagc aatcgctaga taaatgcact ttttatcgtt
      721 cgatggtaga gagaggaatt attactgaaa cgggtttacc gacccaacag gcaattgaaa
      781 atggattagt caaagattac tatgaggacc aaggcttatc atttgatgat tttttaagaa
      841 tctatccaat ttttgaagag tatgacgagg agttatttca atgtatcgat ggttattggg
      901 agatacctgt tgatatgaaa gaaaatttag tttcacaatt ggaatctggc gagttaactt
      961 ttgaagatgc acaacaaatt caagcatatc ttgaagatcg ataagtattg aaggaggaag
     1021 atttatgcgt caaaatatta ttttggaatg tgtcgaaact ggagaacgtc tgtatctcac
     1081 cagcaaaaat aaacggaata accctgaacg gttagaatta aaaaagtatt cgccaaaatt
     1141 gcgcagacgg gcaattttta aagaagtgaa atagctaagt ggtaaaaagg tgacaaaatg
     1201 ggcataccag ttacaatcgt ttctgggttt ttaggggctg gaaaaacaac gctaatcaat
     1261 caagccttgc aagtctcacc ctaccccagg gaagaaatta ttataattga aaatgagttt
     1321 ggtgaagttg ggattgatca caaattatta gttcatagcg aggaacgggt gttacaacta
     1381 aataatggat gtatgtgttg cagtcttcgc agtgatttat tagcaatatt gatttcttta
     1441 ctggaaacaa tagaagaaca tcaacaacca atttcacaaa tcatcattga aactacaggt
     1501 atagcagatc cccaaccaat cattcaaaca cttttaacag cgcctcaact aagaggaaga
     1561 gtatatatcg atagtttatt gactgttatt gatagtgaca gttttgaact tattgaaaga
     1621 gaaccacaag cccttaagca acttgtcatg gcagatcgca tttttgtgtc tagaaaacaa
     1681 aacttgaatt tatctgcact aaaaaaatta cagagagaaa tcaaaagtat taacccttta
     1741 tccgatattc gaatgttcac acatagtgaa acgattcaaa aaaaggattt ttataatttg
     1801 aaaaaattta atcatccctt agtttttgaa agtgattcat cagaaaatca ctataatggc
     1861 aaatactatc atcaacacca tcaagtacat gagtttaaat caattctctt aacttcagat
     1921 caagacataa aaaaagattt tttactccaa tggattgact ggctgatttt aaccaaccaa
     1981 gggagcctgt atcgttttaa aggattgatt gctttacaag agcaggattt tatagttgcc
     2041 atgcaagagg taaacgaaca ggttaatttt caatacacca ctcgacaaaa agaccaaacc
     2101 actattattc taatcggaaa aatgttggat gaagaaagga tcaaacagag ttttgataaa
     2161 ttactggcgg aaagcaaata atgataagta gtgattgggg gtaatttcta atggagaaaa
     2221 cggatatatc tagtgcatat cgacgactta aaagcccgaa tattaaaact agaaaaaggg
     2281 cattaaaaat tatcaaagaa cataaacgaa ataaaatgaa gaaattagca taattaagga
     2341 aggaaaaaga atgaagaaaa tcagtgtagg acttattggg atagcagctc taggattatt
     2401 gggagcttgc tccagcacta atgatgcaaa agtatcgaat gataaagatg gaaaattaga
     2461 aattgtaaca acattctatc caatgtatga tttcaccaaa aacatcgtcg gcgatgaagc
     2521 gaacgtggat ttaatggttc cagcgggatc agaacctcat gactatgagc cctcagctaa
     2581 ggatatggca aaagctcatg atgctgatgt ttttgtatat cataacgaaa acatggaatc
     2641 atgggtacca aaagctaaag aaagttggaa gaaagctggg cctaatgtag tagaaggaac
     2701 aaaagacatg attttattgc caggtagtga agaagaagat catgatcacg gagaagaaga
     2761 tcaccatcat gaactagatc ctcatacgtg ggtttcaccc aaaatggcaa ttaaggaagt
     2821 ttcaaatatc aaggatcagt tagttaaatt gtatccgaaa aaagcaaaag tatttgagac
     2881 aaatgctgaa aagtatttga ctaaactgaa acggttggat gctgactata ccactagtct
     2941 taaagaagcg aaacaaaaaa gttttgtgac gcaacacgca gcctttggat atttagcttt
     3001 ggattatgga ttgaaacaag ttcctattgc tggactaagt cctgaagaag agccatcttc
     3061 tggtcgatta gctgaattaa aagagtatgt taaaaaaaat aagatcaatt atatttactt
     3121 tgaaaagaat gctaatgata aaattgcaaa aactttggcc aacgaagctg ggataaaatt
     3181 ggaagtactg aatcctcttg aaagtcttac aaaagaacaa atggataatg gtgaagatta
     3241 tgtttcagta atggaagaca atctaaaagc gttagaaaaa acaactatgg ttgctggtga
     3301 agaggtagtt ccagaaaaag aagcgaagga tgaaaagaca gttgctaatg ggtactttaa
     3361 agatgccgat gtgaaggatc gcgaattgag tgattataca ggcgaatggc aatctgttta
     3421 tccgttgtta aaagatggaa ttttggatga agttttcgat tacaaagcaa aaatcaataa
     3481 agacatgaca gctgccgagt acaaagatta ttatactact ggctataaga cagatattga
     3541 tactatcaac attaaagaca acaccattga ttttgtagtc aacggtgaac atcatcaata
     3601 cacatacaaa tatgttggat acaaaatttt gaattatgaa aaaggaaacc gtggtgttcg
     3661 ttttaatttt gaaactgatg atgcaggtgc tggtcgtttt aaatatattc aatttagtga
     3721 tcatggaatt gcaccaagta aggcagagca tttccatatt ttctttggtg gagaaagtca
     3781 agaaaaactc tataatgaat tgcataattg gcctactttc tatccagctt cattgtctaa
     3841 acatgaaatt gctcaagaaa tgatggctca ttaaaattca atttttggag gaaatacatg
     3901 gtaagaaaag tattattgtc tatggattcc aagaatattg atgcttatcg gcgttatcga
     3961 tttgcaaagc aaatgagatt aaatgggagt acattagctg atattgctgc aactttacat
     4021 gttagtattg acacagttca tagagtatta aaacgggatc ctgattgttg ttttatcaag
     4081 gtcaatactg gaagaagtag acgtttagca aaacggcata atagcttggc caattaattg
     4141 ataataccag agtagatcat ttttccctta tttggatttg atctgctttt tattatgtct
     4201 tgaattgtca aatcaagcgc aacactttat caaagaagac ttcataaggt gttctccaat
     4261 ctaaacactt tctaggtctg ttattcagtt tttcaatcat ctgatttatc gttgagttat
     4321 ctaccgtagc tatctctttc gattttggca agtattctcg aatgagtcta ttcgtatttt
     4381 aatttgttca ttgttgccaa gggcatgagg atctggaaaa taaaagggca tattattcaa
     4441 tgcttctgta actgcgaaat gattggagaa ttcttttcct ctatctggag tgatcgtttt
     4501 gaccgcctca tttggaatac tgcttaaaag ttcaatcatt ttctcgctca cataagcggc
     4561 tttctttctg gggaccttct ttcctgctaa aaggaagcat gattttctgt ctgtcaaagt
     4621 cactaaacaa gtaccaccag ttttgccagc tacaatatcg gctttccaat gaccaaaatc
     4681 acttctatca tctgcggatt ttggacggcc atggatcgaa tgagtaaggc aaatctttcc
     4741 acgagactcg acatgatttt ttgtatgtct tgtttttcct cgatggtgca atgaacggat
     4801 caggccttta ttgccgtgtg agaggggttc tgtatcgaaa agatcattgc aaattgcacg
     4861 ttagatcgtg ataaaactaa ttgaaaagtc catatgttca tgcttcaaac gattcgagat
     4921 ttcctctggc gaccactgaa aatcgacaaa taaatgacga acaattgacc aaactcacgg
     4981 atcatttaga atgtgataac gtccgcattt ttttcttcga taagcataat ttttttccgc
     5041 tttggaaggt gaatagtttt tttcttgttt cttctcagtt catgtaaaat agtaaacggg
     5101 ctatgctgaa tgtcgttgcc aattatacta atagaaaatc cttcttcgtg aagcagaaag
     5161 attctttctc gttcgagtat agtaagatga cgatactggc tcataattat tttccttcta
     5221 ggagtgttct cgacaaactt atactagttg gatatcgatt atgactcatt tttatgtgtt
     5281 gcacttaaag tgtaaattca aggtatagaa agaggttccc tatgaaaaaa atacctatta
     5341 caatcttaac tggctatctt ggttcaggaa aaacgatact tctaaatcat attctgtacg
     5401 gtgatcacgg aaaggaaatt gctgtcattg taaatgacat aggggaagta aatgttgatg
     5461 gggtattagt cgacaagaaa ggttttaaaa gaactggaga aaaacttgtt tcgatgccga
     5521 atggatgcat ttgttgtaca ttacgcgaag atttgattgt tgaactagat caattagtgc
     5581 aattttctga cttgaatcaa attgttattg aagcttcagg aatcagcaaa ccaattccaa
     5641 ttattcaagc aattatatta gctaaaaacc ctttagacat tgatttgacc caacgattag
     5701 agattggtgc cattattata gtagtggatg taaaacgttt ttgggataat ttcgaaacgg
     5761 gtgaaaagat tcaggaaaga aaagctgaag gtaatgtagt tgaagaagaa caggatattc
     5821 gtggcttgtt aattgatcaa ttcgagtgtt gtaacatatt acttttaaat aatagtgact
     5881 tagtgtcaac caagacgatt tacaaaatta acggtctaat tgctaagtta cagtccaatg
     5941 ccaaaataat taggacaatt aaaggtgaag tagacttatc gaaaattcta aatacaaatc
     6001 tttttaatat agatgagact tcctattcag tgggctggtt caaagagtta gagcttggct
     6061 atcaaaatca tattcctgaa acagaagaat atggtatctc aagttttatc tatcgaaatc
     6121 ggttactcct tgacgcacaa aaatttgccc atcttacgga taaaaatttt cctggcaata
     6181 ttacaagagc aaaagggttt ctttggtttg cccaacatag tgatttttcg acgatctttg
     6241 aaacatcggg tagctataaa agtattgaat cattaaatta ttgggtagct agtttaccta
     6301 aaattgagtg tcaaatgatt atacaaaaaa atcccgaatt gaaggaagcc tggcatccgt
     6361 tgtttggtga acgcattaat aaactagttt ttattggtat gaagatggat caacaatgta
     6421 ttaaggaaca acttgagggt tgtttaactg attctcttaa tgcagataat ttccgaaaaa
     6481 tttcaccatt tccatggaaa aagtctaata tgtttagcca gatatcgtta atgaaatttt
     6541 taagaaaagc tggtgtcaag ataggcgatg tcataattat tggcattttg attattacct
     6601 cttttattcc cttatttgta ttctctatag aaaaggagaa tgaaacaact gaagatgcta
     6661 tcaaatatgc catcgttcga attgatggca aggaaatgga tcgcttcgat ttggataaga
     6721 tcaatcataa actcgtgaat tatcatcctg cgaagggaca atataatatt gttgagatta
     6781 aagatggaag aattcgagtt aaagaagata atagtcctga tcaaatcgcg gttagaactg
     6841 gatggatctc aaaacccggt caaacgagta tttgtttacc ccataagtta gttattagta
     6901 ttgagaaaaa agaaagcaca gattactaca tttactaatc tggaaataga tgaggaggtt
     6961 tttaataaga aaaagatatt attattaata atagcagggg ctttattaag tgtgttaagc
     7021 gcttgtggac aagaaaaaga agcggtgaca acaggcacaa gtatctttaa cgggacctgc
     7081 aatggtttag ttaacagtcg gttagattac ttattttggc tcttcgtcaa tgagtgttgg
     7141 tgaagaaatt caatagaata atcgagatac tcacacggct agacagctat cttgtctagc
     7201 cgtgttgttg ttctggcttt agtcagttgg gtagtattct tagttttctt aaaatatttt
     7261 cctcgatata aaaagattcg ttctcggtag ttttgaaagt ttctatagcc ataggcaatt
     7321 cgttttagta ctttgatatg gttattcaaa cattctatag ggccgttgga atagggaacg
     7381 cgcaacgctc gtttgatttc tgtttggtat ttcttgaagg tcgtgaacac tggttggtag
     7441 tgctctggca actccttgta gtcttctttt aggagcgcga cgaattcagg aacatcttgt
     7501 cttctgataa cggaaagaaa atcctggtag acttcgtagc ctcgctttaa ggaatcatca
     7561 taagataata gccgatcgac tatctctgct tctgtcagat gatctcgaaa tgacggacgc
     7621 cagtagcgat gctgaccttt caattcccac gcattctttt gaagtagttt ccagtatttc
     7681 tttaagtgct tcgaccgacg atcccctttt ttaaaggtct tcattacttg aattcggtgg
     7741 ttggtaaaag cccgaccaat gtgttggcta atatgaaagc gatcggcgac gattcgggca
     7801 ttcggaaaac actctttcac caatgtgcga tagggtgtat agaaatctga aaccactaga
     7861 cgcacttgtt cacggacttt tatagggtag cgcaagaaat agtcgcgtaa aggattgagt
     7921 tgccgatttt ccacaatatc aatcagttga tgggtcttgc cgtccatcat gacaaagctc
     7981 atggctcccg agacattttt cactgatttg aattcatcga aacacaccac ttctggtaac
     8041 agctggctca aagaaatggt tttaggccga aggctgcgaa gtattcgaat gacagtggtc
     8101 ggagaaatgt gcttcatacg tgcgatcaaa gacatagaaa gaggttcgct taatagctca
     8161 agaatggctt gtttgactct gcgagcaatg gagcagcgac gatcactaac tgaatcttct
     8221 gctaaaaaag tctttccaca gtgtttacat ttgaagcgct gtttgttaat ccgcagaatg
     8281 gtcttgtagt tactgacatc attcaataag atggatactt ttttccagcc ctatttaatg
     8341 atcgtttgtt cattttgata gtggcgatga aagcattcct ccggttgata ggtcaatgtg
     8401 ccatcaaaag caaaacatgt ttcgtcattg atgcgctctt tgcgaaaggc atcctcatga
     8461 aaggtaagat ttaggtctag aatatctagt aactctttgg taaactctga ataggacatc
     8521 ttgaaacact actttgaatg gtttttgtcg actttattct aaaggatttt aaggtgtctg
     8581 tttgcgttta ttagaaaaaa gaaaaagtat tggcaacaga aggcgtcacc aacactagat
     8641 attatagatc ccttattttc ttcgcttcaa attgtcaagg agtcatcata aattttgtat
     8701 ggattctttt gtttgggtag tagtcttcta aataatcttg gattaacacc ttcatctcta
     8761 ttttactttt atatttgttg gagtcgatta attctcattt gattgatcga tgaaacgctt
     8821 caattactgc attgtcaaca ggagtcccag tttggctcac tgaatgtgtg atgtggtggt
     8881 cgttcaacag ctcttgataa ataaaagacc gatgctggct acctctatcg gaatgaatta
     8941 ccgtaggcaa ttctccttaa taaatttgta atggacggac gacaatctct ttttttatgt
     9001 gtgaatctac catatagtaa cgtagaatac gagttgctaa gtcaatatag gtggacaaat
     9061 agaggcgacc gtcattacat ggaatgtaag taatatccat cacccaaaca gcatcaattt
     9121 ggttctgttc aaattatcga ttcaacattt ttttaccatt tctttgattg ttttttctct
     9181 accatgagaa gatttgcgtc gatgaaatcc tcttgtgcat aaattcattt cacgcataag
     9241 aacagctaca acacgtttat tgatttcgat tccttcatcg taaagttttt gagtgatccc
     9301 tggagatccg tatcgatctt tgtgttcgta gaacactcgt cttcctcttt tagtcgttta
     9361 ttttcctgtt caagaatgta gaaatctgct ttatcaggta atctttttcc ttgtccagca
     9421 aaagcatttt tcccgtatcg acgatattct gaaacccatc gggaaattgt tggacgagag
     9481 atattgtatt cttcagcaat tttcttggta gttacttttt tctcaaggat atcctgtgaa
     9541 atctttagtt taaatttacg atcatatgtt tttctcaata gagttcattc cttccggtaa
     9601 cgtaactgat agtcaagaaa agtgtatcat agcccaactt agtatcaggg tttgtcaact
     9661 aaactgtgga agttgataga taagagtttt taacaaccaa aattctcttg actatttttg
     9721 aaatcagaca aattttgatt ggacacatag ttgaataaat ctaccgtcgt tacggaaggt
     9781 aaatcgcatg cttttcaatt ctagaggaga agtcctcgtt gaagtaccat agcccagttc
     9841 tggattcgac ctcctgccca cttcatacgt aaataaagta attttaggag tgagactgtg
     9901 acaaaagtta tttttaagct aaaaacgaat taaaaaacgt tgataattaa aatcattcgt
     9961 tttagattct atgaatcatt atagaacgct gtaaaatcga tactttataa ttataaagaa
    10021 gatagtttca aatttattgt tcgaaatttg aaactatctt tttacttatg tcacagactc
    10081 ccttagttga gttattcttt aatttcgaat ttcgagatag ctagatatat tgcaagcata
    10141 cattcttgat aacaatcttg atcatatttt ccagttctcc aggattgctt atgaatcaaa
    10201 gagctaaatc ttttgagaat tgcctcaaca gcttcttcat ttccagcctt tgctaaaatg
    10261 aaatcttctt ttaaagaatt cataaatcta ccttcttccc atggacttat aatctatttc
    10321 tttgggaagg aggagaacga gcaaagaagc atacaaaatt agttattatg cagatattaa
    10381 accagcattt tttgaatttt tttgtaaagt cgatgtttta aatttgtcgc accctgtctt
    10441 gtaatcccca gaacctttcc aatttcttta tcagttttat caaagataaa tttttcgata
    10501 agaaaaattt gttcaggttc tttcaacaaa ggaa
//
LOCUS       DAZUMV010000094        10467 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000094 DAZUMV010000000
VERSION     DAZUMV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.095"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..644
                     /locus_tag="ACG8FK_002057"
     CDS             21..644
                     /locus_tag="ACG8FK_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097647.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1094..2143
                     /gene="trpX"
                     /locus_tag="ACG8FK_002058"
     CDS             1094..2143
                     /gene="trpX"
                     /locus_tag="ACG8FK_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5097648.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2140..3021
                     /locus_tag="ACG8FK_002059"
     CDS             2140..3021
                     /locus_tag="ACG8FK_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5097649.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3024..3794
                     /locus_tag="ACG8FK_002060"
     CDS             3024..3794
                     /locus_tag="ACG8FK_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097650.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4073..4927
                     /gene="aroE"
                     /locus_tag="ACG8FK_002061"
     CDS             4073..4927
                     /gene="aroE"
                     /locus_tag="ACG8FK_002061"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS5097651.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5105..6268
                     /locus_tag="ACG8FK_002062"
     CDS             5105..6268
                     /locus_tag="ACG8FK_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5097652.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6385..6795
                     /locus_tag="ACG8FK_002063"
     CDS             6385..6795
                     /locus_tag="ACG8FK_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5097653.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6969..9197
                     /gene="topB"
                     /locus_tag="ACG8FK_002064"
     CDS             6969..9197
                     /gene="topB"
                     /locus_tag="ACG8FK_002064"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5097654.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9368..9652
                     /locus_tag="ACG8FK_002065"
     CDS             9368..9652
                     /locus_tag="ACG8FK_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097655.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9812..10048
                     /locus_tag="ACG8FK_002066"
     CDS             9812..10048
                     /locus_tag="ACG8FK_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5097656.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10045..10443
                     /locus_tag="ACG8FK_002067"
     CDS             10045..10443
                     /locus_tag="ACG8FK_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5097657.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3645 a         1763 c         2220 g         2839 t
ORIGIN      
        1 gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt agttaaatcg agagtacaag
       61 aaatgaatga acatttctca gtgtcattcg cagaaaatcc aagtaagtat tttcgacgtc
      121 ttgtttttct gctcatctta tcattgctgt taacagttgc aatgttttta ttgccggaaa
      181 aaatccgaga agagcaagta aatagtctcg tttattcgtc cataccaacc agcaaattga
      241 aaccttttga ttacagcaaa gctgatcaga aaatcaaaca aagccgggcg atatcggtga
      301 tgttcagcaa accgaacggg aaaacctatc aggacatgct tgatgttttt aatgatcctg
      361 agcttatgga agaattgaat cgaccgatct tttattatcc gatcgtttat gatgtacatg
      421 aactggaaca aaaatataac atacgaacgg atgaagtgac atttattttc tttgatggag
      481 gaaaagaagc aaatcggata actgcgggta agggaggaat aactgatttt gataaggagt
      541 tgatccctga actgaatcga cttccgttag ccaatatcaa acaattagaa gaagaattgg
      601 atagaacggg agtaacttcc caaaattccg agattgctaa ttgagacatt ggcgcgatga
      661 aacggtgggt ctatatagcg ctatttttaa ttatcagaac aagtgatgtt tgatcttcaa
      721 aataacaaat aaaaaattaa aaaatatagt tgacgaaatt caaaaattcg attagactta
      781 aatcaattct taaggaaagg gtgacctgta atgaacttat caaaacgtat ttcagcagaa
      841 aacaaaaaaa attggcgcag ctggcgtaga taagttgtat taatggtgag actttcccat
      901 agatacaact ttttagagtg catttctaaa gcttgtatct atgctagaca tgaacattga
      961 ctgccgcata gattagaatc tatgcggttt tttgtgtaaa gacaatgacc gctttggcca
     1021 ttgtcttttt ttgtagactg gatacttgcc tgattataat aaataaaaag attgactttg
     1081 caggaggaaa aaaatgaaaa acaaacgatt aataacagtt gtagtaatta ttttagttta
     1141 tttagcaggt gcatttattt atggaaaagc ctcaggcgaa aaagaaagta cttcgacgaa
     1201 ccagacaaaa aaaacaacag ttgttggggt attgcaatac gtgagccatc cggcattaga
     1261 tgagatttat cgaggaatca aagatggtct gaaagaagaa ggattaaaag aaggagaaaa
     1321 tcttgagatc aaatttcaaa atggacaagc cgatcagagc aagctagcca caatgagtca
     1381 acagctagta caatctgatc cagatgcctt gattggaatc gcaacacctg ccgctcaatc
     1441 attagcaaac acgacttcat cgatcccagt tgtgttaggt gctgtaacgg atcctgtggg
     1501 ggctggatta gtcgcttctt tagagaaacc tggtggtaat gtaacaggtg tttctgatca
     1561 gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg ttgccaaatg caaaaaaagt
     1621 cggaatcttg tatgcttctt cagaggataa ttcaaaatac caagtagcac aagcagaaga
     1681 agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg gtgccttcca ctaatgagat
     1741 tgcacaaacg gttcaagtga tgagccgaca agtcgatttt atctatgttc cattagataa
     1801 taccattgcc aatgcgatgc aaacagtggt aaaagaagca aacaaagcaa atatccccgt
     1861 cattccatct gtggatacaa tggtggaaca aggtggacta gcaacaatcg ggatcaatca
     1921 atatcaatta gggcttcaaa gcggtaagat ggcggcagaa cttgcgagtg gaaaagaaaa
     1981 accggaaacg acgcctgttt atacatttga tcaaggggat acagtgatca atcaatctca
     2041 agctgatcgc ttaggaatca ttgttcccca atcgatgaag gaaaaagcca aaatcattac
     2101 agatgactcg cagcaagaaa catctaagga ggatgacaaa tgatcgtatc agctatttca
     2161 caaggcatgc tttgggcaat attaggatta gggattttca tgacttatcg gatcttagac
     2221 tttcctgata tgacgacaga agggtccttc ccattaggtg ctgctgtctg tgtgacagct
     2281 attactcatg gggtagcgcc gattgtagct acattattag gcgtaggtgc tggcatgata
     2341 gcaggactcg ttacggggtt actttataca aaaggaaaga taccggttat tttagcaggg
     2401 attttagtga tgtcaggatt gaactcagtt attttgtttg tgatgcgaac accaaatctt
     2461 tctttattga atcatccgat tctacaagac agcttccatc gctttgggtt acctgattac
     2521 tttgacacag tccttcttgg cttcatctct ttgggcatct tgattgctgt actgctattc
     2581 ttcttcaata cagatttggg tcaaggctat attgcgacag gagacaacga aacgatggct
     2641 cggtcgttag ggataaaaac cgatcgtatg aaaattttag gtttaacttt gtcgaacggt
     2701 gtcattgcat tatccggtgc gctgattgcc cagaacgacg gatatgcaga tgtaaataaa
     2761 gggattggcg taatcgtcat cggtcttgct tccatcatca tcggtgaagt gatcttccaa
     2821 gaattgacgc tagcagaaag attgattgct attgttgtcg gcagtatcat ttatcagcta
     2881 ttgattttag tagtaatcaa actagggttt gacacgactt atctgaagtt gttctccgct
     2941 atcatcttag ctatttgtct gatgatcccg caattgaaga aagcactgaa cttaaaaacg
     3001 ggttttgaga aggaggcata gtaatgactg atatattgac aatcaaaaat ggcagaaaag
     3061 tcgtaaacaa tggaatgaat gaagagaaag tactattgga tgaaattaac cttacaatca
     3121 gagaagggga cttcatcact gtccttggcg ggaatggtgc tggaaaaagc acactgttca
     3181 atacgatcgc tggtacgctt caactcagtc aaggatcgat ctcttttaaa gatcagctga
     3241 tcacaaaaga ttcagaagaa aaaagagcac gattcctttc tcgagttttc caagacccca
     3301 agatgggaac ggctcctcgg atgacagtag cagaaaattt gctacttgca caaaaaagag
     3361 gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca aaaagaagat tttttcagac
     3421 tatgtcaaga agtcggtaat ggcctagagc aacacttgga tactccagcg ggtgaattat
     3481 ctggtggtca acgccaagca ttaagcttgc tgatggcgac gatcacagct cctgagttac
     3541 tattattaga tgaacatact gcagctttag atcctaaaac agctaaagca ttaatgcgat
     3601 taacaaatca gcggatcaat gaacaaaaat tgacttgtct catgatcacg catcgaatgg
     3661 aagacgcctt gaaatacgga aatcgtctga tcgtactcca aaaagggaaa atcgttcaag
     3721 atatttctga aaaagaaaag caacatttga caatgggcga ccttttgcag ttctttgaag
     3781 aagtagtgga ttgatttcga cagaaaaatt cctttcagta gaaggacttg aaaactcatt
     3841 gacatcttcc tttgattcat cctatactta aaacaataaa caattaaaca tatttgggtg
     3901 ttttgtagga tcacgtagtc aatgatttta acaattaaat agaatattct ttgctctgac
     3961 aagtaaacga tgagaaaaga atttgctact ttgccaccta gacgtttcgt actttttcgt
     4021 atcaaacgct aagtggcttt ttttattgaa gtaagaacaa aggaggaata agatgatttc
     4081 tggacaaacc aaattagctg ggctctttgc tgatccggct tctcacagtt tatccccatt
     4141 gatgcacaat actgcttttg aagcaaacaa catcgatgcg gtgtatttag ctttccaagt
     4201 aaatccaatg aatttgaagc aagcagtcga aagtattcga acttttgata tgttaggtgt
     4261 aaatctttcg atgccgaata aaacagcggt tattccttat ctggatgagg taagccagga
     4321 agcgcaattg atcggagcag tgaatacgat cgtgcacgaa aagggtcgtt tgattggcta
     4381 taataccgat ggtgcgggct tcatgcaatc cgtgcaagaa gctgggattt cgatcaaaaa
     4441 gaaaaaaatg actctactgg gagctggagg tgcagcaaag gcagtcactg ttcaagctgc
     4501 gttagacggc gctgaggaaa tcgtagttta taaacgaaac aaccaaacat tcgctcaagt
     4561 aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt aagataaaag ttcgtgactt
     4621 tgccgatacg catcaattaa gaaaagactt gaaagaaagc tgtcttctag tcaatgcaac
     4681 agatatcggg atgggaaaga aaatcaatca gtcaccgctc ttagatataa gcatgatgca
     4741 tgagaatcta gcggtttttg atttgattta tgcacctaga gaaacacgtt tattaaaaga
     4801 agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc atgttgatct accaaggagc
     4861 aatcgcgttt gaattatgga caaaggaaaa gatgccgatc catgtgattg aaaagctgtt
     4921 tgattaaaaa aggataatag ataaagatct tttattctcg cataaacggt ctcaagaaaa
     4981 gtaatccttt atgtatcgaa gcatttattt cgttattttt tattaaatta tcaaaaaatg
     5041 ataggctttt gtcgattggt tatcgtatcc tttatttatc agtaagaaac gaggaggaac
     5101 cacaatgagt cggatggatc gctataagga catccataag aaggcgaaac ctctgaagcg
     5161 ggatacgggg agtatttttg ctagaagaga acgaaaacga acgcgtatgg aaaatgagga
     5221 agatgctacc cgtatttatg aaagagagca gcattcattt gaacatttga accaatacga
     5281 gcaacagata tacagagatg caccaagagc agaagaacaa caaaaaaata aaaaattttt
     5341 caatcggcaa aagaagaaca aaccgttaaa acctcgaaga aagcgatcgt ggaaaaaaat
     5401 cattctatcg attttgcttg ttttgatcct ttattcaggt atctcatttt ttattggtaa
     5461 atatgtagct gaacatgaca caagcttaga ggcagcgact tcagaaacct ttcatgggca
     5521 aaaagcgacg aatggtgcgc ataatatttt gatccttgga agtgatacac gcggagaaga
     5581 tgccgggcga gccgatacga tcatggtctt acagttggat ggtccttccc ataagccgaa
     5641 actgatttct tttatgcgtg acagcttcgt tacgatccca ggggtaggac aaaataagat
     5701 caattcagct tatgcatacg gaggagccga tctagtgcgt caaactttag tagaaaattt
     5761 tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa tcgtttgaaa aagtgatcga
     5821 tgccttattc atgaacggag tgaagatcga tgcagaaaaa gatctgaatc tggatggtgt
     5881 agatattaaa aaaggggtcc agaagatgga tggacatgtt cttttgcagt acgcacgttt
     5941 ccgaatggat gaacaaggag actttggccg tgtcagacgc caacagcaag taatgaacgc
     6001 tattttcagc caattgaaga atccattgaa tctgatccgt gcaccttatg cagctggtaa
     6061 agctttagga tatacatcca cagatgtcac tgcttggttc ttgacgaaaa acctcttttc
     6121 gattgcacga ggagctggtg gcattgatcg aataagtgtg ccagtagacg gttcgtggaa
     6181 ttatgggcaa agtgattatg ctggcagtat tcttgaaatt gataatcaaa tgaatcaatc
     6241 tgctattaca gattttttga ataaatgatc ataaaaaaga tcaagaagaa gctgcgcaag
     6301 gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa acgctttctt atggtagatt
     6361 taaccaagag aggagctgtt tacattggat atctatctga acttcaaaaa tgagtcgcgt
     6421 gaagccatcg ctttttatga aaaagttttt cagactgaag catcaggtat caccacatac
     6481 ggtgaaatgc ctggaataga acaaatgaac ttgccagaag aagcaaaaaa tctaatcacg
     6541 aatgcacggc tgaacattta tgatacatta gttatgtttg cagatacgcc agaaggaatt
     6601 ggtcctgaat tagttgtggg aaataatgtt tctttagtca tccagacaag aaatgaagaa
     6661 gaaacgaagc gcctatttga aagcttagct gagggtggta agattgagat gccactggat
     6721 gctgttccgt gggctcagtt gtatgggatg gtaactgatc gatttggcat caactggcag
     6781 attaaccaag agtgacagct aaagatgaat attttctgga aacatcaagc agcaaacgga
     6841 tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac tatacggtat ctttttttta
     6901 ttgccagatg cgaaaatatg ttccataata gagaagtccg caaaaaatta agtaacggag
     6961 gtcagaagat gaagacggtg atactagctg aaaaaccttc acaagcaaaa gcctatgccg
     7021 aaaccttcca acagcaaaag cggcatgaag ggtattatga aatcagcgat ccattgtttg
     7081 ctggtgaagt aaccatcact tatggttttg gacatttggt agatatggtt gctccaggag
     7141 cttatgaaga gcgttgggca aaatggtctt tagaaaattt gcctattttc ccagaaacct
     7201 tccgttacga agttccaatg gacaaacgtg cacaattttc gatcgtaaaa cgagaattgc
     7261 agtcagccga taccatcatt attgcaacag atggtgatcg agaaggtgaa gccatcgcgt
     7321 ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa gaactattta cgtttatgga
     7381 tcaattctct tgaaaaagaa gcaatctatg aaggttttaa gaatctccaa cctggaaaga
     7441 actattttcc aaaatataaa gaagcgcaag caaggcagaa cgctgattgg ctgattggaa
     7501 tgaatggaag tccattatat agcttgctac ttcaacgaaa agggattgat ggcagttttt
     7561 ctcttggtcg tgtacaaaca ccgacactct atatgattta tcagctgcaa gaagaaatca
     7621 aacacttcaa aaaagaaacg tactttgaag gagaaggttt aattacgaca aacaaagggc
     7681 agttttctgg aaaaatcgta ccaaaaaaag catttaaaac ccaagaagag ttaacgaagg
     7741 aaatagaaaa acttggagcg catttaggta agcaaaacgg acggattctt gaagttacga
     7801 aaaaggaaaa gaggttgcat agtccacgtt tattttcgct atctagtttg cagacaaaga
     7861 tgaatcagct aatgaaagca agtgccaaag ctacacttga tgctgctcaa ggattgtacg
     7921 aaagcaagtt tctaagctat ccaagaacgg attcctttta cattacagaa aatgagcatc
     7981 agtatctggt taaacatctg caaaattata aaaaattttt aggaatagaa gaagtacaga
     8041 caactgagat caaagcaaag aaacgatatg tcgatgcgaa aaaagttcaa gaacatcatg
     8101 caattattcc gacaaagacg attccgactc ctgcacgctt tgcgaagctt tccaaattac
     8161 agcaagcaat ctatttgcaa gttttgcgca ctactgtggc tatgtttgct gcagattata
     8221 tctacgaaga gacgaccgta atgacgggcg tacagcaatt aaagctaaaa agcatcggaa
     8281 agatcccatt acagcaaggt tggcaggaaa tcttacgcat caagtcaaaa aagaaagaag
     8341 ctcagactct tcctcctatc gtagaaaatg aagaagcaga agtagaactg aaaagtatgg
     8401 agaaagaaac acaacctcca aaaccctata ccgaaggaac gttgatcaca gcaatgaaaa
     8461 ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat tctcaaagaa gtagaaggaa
     8521 tagggacaga agctacacga gccaatatca ttgaaaatct taagcagaag cattacatcg
     8581 aagtcaataa aaacgaaata actgttacgc caaaaggaat cactctttgt aaggcagtag
     8641 ccaatgaacc attactgacg agcgcagaaa tgactgcgcg ctgggaaggg tacttgagaa
     8701 aaattggtaa acaagagggg acaccatctg tttttttaga gaatatcaaa aaattcatcc
     8761 tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt agacttttca gatgagatta
     8821 gcgggacacg tcagataaaa gcgatggaga agaaaaacga tcaattagga agatgtccaa
     8881 aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc tacatgcttg aatccggaat
     8941 gcgacttcaa attgtggcca acagtagcta aaaagaagct gactaaaacg aatctcagag
     9001 atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg ttttactggt aaaaaaggga
     9061 aattcgatgc agttttagta ttaaaagaag atatgacaat cggtttttct tttccatgga
     9121 tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga aacatcaaga aaaaataatg
     9181 aaaacgaagt agagtaaatg aaaacaatga aatagcggaa tatttattcc gaaaagtgag
     9241 aatataaata ccaataaaac taaaaaatat cagtgaatca agcaaaaacg ttggggtagg
     9301 ctgattttat atagatagca aggagttttt ttatttgaaa aaagtgcatt catttttaaa
     9361 ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa gcatttctaa agtatcctga
     9421 ttacgggacg aagttgttgt cattgacgca caatcaaaca gcatattcac caacgttgat
     9481 tttatcgact ttgactgcag gagctaaaag cgctacttat ttttttcata aaacgacaag
     9541 catgctaaag cgaaagaaac agcgaaaaca attgttggtg gtggtagcgg ctatgatcat
     9601 tatttatttt ctaccagtta ttacacaagt atttcatcaa tattttggtt aagaatacga
     9661 gagtttgtga caaaagtcat gtcacaaact tttttctttg tcattttttc gtaagaaaaa
     9721 gcaaaataaa agtatataca ttgattaaaa gtatatactt ggatataatg tacttactat
     9781 agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa atgaaaaagt ataaaattag
     9841 aaaatctggc aatagcgatg taacaactat cccaccagaa gtaaaagaat tcatgggtgt
     9901 gcagacaggt gacgcaatca gttatgtttt tcaatctgat ggttctgtac gaatgatcaa
     9961 agcacaagaa gaacctgata ttgattcatt agttgattcg atcatgaatc aatatgaaga
    10021 cgcgttaaaa gatttggttg atttatgagg tatctttcga ctaaagaaat tatcaaaatc
    10081 aacgcgaaag ttattttacg tcactcgcca ggcgaaatga tcggtgtaaa agatgcaaat
    10141 gcattagata tggccgtcaa acagccttca caagcagtat tcaatcaaga actctatcct
    10201 gaaatctatg aaaaagcggc gattttagca attaatttag ctaaaaaaca tccttttcat
    10261 aatggcaata aacgaactgc tcttgttgcc atgttgttgt ttcttcaatt gaataattgt
    10321 ccagttgctt tttctcgtca agaagctgtt gattttatca taatgattac cactagttca
    10381 ttggatttcg attcactaaa gtccaagata accagttatt tacgacaaac cgcaataaaa
    10441 tgaacgaatt aaaaacttta tttctca
//
LOCUS       DAZUMV010000095        10178 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000095 DAZUMV010000000
VERSION     DAZUMV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.096"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            347..2182
                     /gene="tdc"
                     /locus_tag="ACG8FK_002068"
     CDS             347..2182
                     /gene="tdc"
                     /locus_tag="ACG8FK_002068"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5097658.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2487..3965
                     /locus_tag="ACG8FK_002069"
     CDS             2487..3965
                     /locus_tag="ACG8FK_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5097659.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3931..5262
                     /locus_tag="ACG8FK_002070"
     CDS             3931..5262
                     /locus_tag="ACG8FK_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5097660.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5376..7928
                     /locus_tag="ACG8FK_002071"
     CDS             5376..7928
                     /locus_tag="ACG8FK_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS5097661.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7935..9374)
                     /locus_tag="ACG8FK_002072"
     CDS             complement(7935..9374)
                     /locus_tag="ACG8FK_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5097662.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9349..9531
                     /locus_tag="ACG8FK_002073"
     CDS             9349..9531
                     /locus_tag="ACG8FK_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097663.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9720..10082
                     /locus_tag="ACG8FK_002074"
     CDS             9720..10082
                     /locus_tag="ACG8FK_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS5097664.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3256 a         1659 c         2150 g         3113 t
ORIGIN      
        1 ttataaaatt tggaaaacag ataattctat taaaaaataa attgcgaatt tttaacaata
       61 taaaaaaata aaaatatgtt tagtgaatga aggacagtca ttttatttgt ttgttcgctt
      121 tttttaggtt atctatttta gaagactgat ttattgaaaa attgttcgct tttttgcgtt
      181 gaattatcag aaaaatccga ctttatttat ttttgtttgt aaaaatctac gtggatttga
      241 tgttataaaa aaaaaattct catttttggc gctatagtga agaggtgtta cagataaaca
      301 catgtatctg caagacattg accaacaaaa tatggaggga agaaaaatga aagatatgga
      361 tatcaaggcc gtctttatag gcgacaaagc tgaaaatgga ccagtttata aaatgttgtt
      421 gaacaagatg gtagatgaac atcttggatg gagagaaaat tatattcctt cagatatgcc
      481 tgcaattagc gaaggagata agctcactcc agattatcta gccacccgcg accatatgat
      541 agaggtcttg gatgaagtca gtcagcgatt acgcgcagga tcgatcccat ggcattcagc
      601 aggccgttac tggggacaaa tgaatgctga aacattgatg cctgctttat tagcttacaa
      661 ctatgcgatg ctgtggaacc caaataatgt tgcattggaa tcttctatgg caacttcgca
      721 aatggaagca gaagtaggac aggattttgc ttcattattc aatatgacag acggatgggg
      781 acacattgca gcagatggtt ctattgctaa tcttgaagga ttatggtacg cccgttgcat
      841 caaatcgatt cctttagctg tcaaggaagt gctgcctgaa aaagtgaaaa aaatgtctga
      901 gtgggaactt ttgaatttat ctgttgaaga aattttggaa atgaccgaaa gctttacgga
      961 cgaagaaatg gatgaggtca aagcagcttc ttcccgtagc ggaaaaaata tccaaagatt
     1021 agggaaatgg ctggtccctc aaacaaaaca ctattcttgg atgaaggcgt tagatatttg
     1081 cggagtcggc ttagatcaga tggttgctat cccagttcaa gaggattatc gaatggatat
     1141 taatgctttg gaaaaaacaa ttcgtgaatt agctggacaa aaaatcccaa ttctaggtgt
     1201 ggttgctgtc gtaggaacga cagaagaagg tcaagtagac agtgtagata aaatcgtcca
     1261 attaagagaa aggttaaaag atgaagggat ctatttctat ttacatgttg atgccgcata
     1321 tggcggctat gcgcgttcac tgtttctaaa cgaagcagga gaatttgtgc cgtatgcttc
     1381 cttagctgaa ttctttgaag aacatcatgt tttccaccac tgcgtaacga ttgacaaaga
     1441 ggtatatgaa gggttcagag ctatttcaga agcggattct gtcacgatag atcctcataa
     1501 gatgggctat gtcccttatg ctgctggagg aatcgtaatc aaacataaaa atatgagaaa
     1561 tatcatttct tatttcgcgc catatgtgtt tgaaaaatca gtaaaggctc cagatatgtt
     1621 aggagcttat attttggaag gatcaaaagc tggagcaacc gccgccgcag tctggacagc
     1681 acatcgtgta ttgccattga atgtgaccgg atatggtcaa ttgattgggg cttcaatcga
     1741 ggcagctcaa agattcagag aatttctcga tcatctgact tttactgtta aaggaaaaac
     1801 aatcgaagtc tatccattga atcatcctga ttttaatatg gtcaattggg tattcaaaga
     1861 acaaggctgt acagatttga acgctatcaa tgaattaaat gaaaaaatgt tcgatcggtc
     1921 ttcttatatg gatggcgatg tttatggtga acggtttatt acttctcata ctacatttac
     1981 acaagaagat tacggtgatt ctccgattcg ttttgttgaa agaatgggtc taacgaaaga
     2041 agaatggaaa aaagaacaga aaatcactct gctacgtgcg gctatcatga ctccttattt
     2101 gaatgatgat cgaattttca atttttatac aaaaaaaatc gccaaagcaa tggaaaagaa
     2161 actaaacgaa atcatccaat agaactgatt tttgtgtgaa agaataacaa gagacatatg
     2221 gagaacagac aattatttgc ttcatcaaaa caattgtatt tccccctgtt tttcatatat
     2281 tcctcgccaa gatattcttt tttataaaac gttctataag ttggatcgtt cgtttcatgt
     2341 atcaaaccca gttcactaag atcgttcaat ggatgtttcg gtgatactgg gttttgtact
     2401 gaatcgatga aaccttgtca tttctgttct tgaacaaaaa taaaagaaaa caaaaacata
     2461 tgtcgctatt gaaagaagga ataaagatga aagatactgg ttcatcaaag caaatcacga
     2521 ccatgacctt tatcggaatg acctgcgccc ttgtagcaag tgtacgaaat attccagatg
     2581 tagcagcaac gagctggacg atgatttttt acatgctggt tgcagtatta ttgtacgctt
     2641 ttccaatctc attgatctct ggtgaatttg ctggaatgtt tccccaaaaa ggtggtccag
     2701 aactttgggt atcgaatgct ctaggaaaaa aatgggggtt cgttgtctca tggctgttat
     2761 gggtccaaat gtttcctggg atggtgatgg tcgcttcagc actcgctccg ttgtttggta
     2821 atattatcga taatgtgccg cttggtttga acagcaaatt tacattagtt gtcattttag
     2881 tcgtttattg gatcatcact tttttgaatt tgaaatttga tatggcaaaa atcggcggaa
     2941 aagtcggtgt ttggttagga ttgtacgtgc ctttgacgat gatgctctta ttagggtttg
     3001 ctgcgtggat caaagttggg atcgtaccaa ccggaacatt aggcagtttt tcatgggaca
     3061 aattaatacc agatactacg actgcatcct catttgttta ttttgcgccg atcatgttta
     3121 tttttactgg tatcgaaatg tcctctgtgt atatcactcg attggaaaat ccagttaaaa
     3181 cgtatattcg cggagtattt gccgcgttaa tttttatatt tttcgtcaat attttgaatg
     3241 ctttagttgt agcaaatgtc gtaccaaaag gacaaatgga attgaataat atcgcgcaat
     3301 ctatttcgat ttattgccaa attcttggtt tgccacatct tattgtgaat ctgtttagtt
     3361 tacttgtctt cattggtgta gcagtccaat tatctgcttg ggcctcagga ccagcaaaga
     3421 cagtgacaga aagtgctaga aaaggtgcgt atccaccaaa attcaatttt tggaagacga
     3481 atcaatttga cgtatccaaa tccgttattt tgactcagtc tgtcattatc tcaatctttg
     3541 cactttttta tttgctgatt cctggcgtca atcaagcatt tttgatgctc gtaaattcaa
     3601 ctactgttat ctactgtatc gtctatgtca tcatggggat cgcagttttg agattgcgtt
     3661 atacacatgc aaagttgaat cgaccattta gaattggaaa aaaagagaaa aagagcaatc
     3721 ttggagtttg gactgtcgtt attgttttat ttgcggcaat cggattttct gtcggactga
     3781 cgatgaaagc tggaacatgg atcaatctaa ttgcagtcac agccatcagt gtcattttgt
     3841 ttgtagtacc actctgtatt gaaaaaataa agaaaccttc ttgggaacag gaagtcgcca
     3901 tgctgcttga aaaagaaaaa ggtgaacttg atggaaaaac cggcaatgaa acggactcta
     3961 actaatcgaa atatccagtt aatcgcatta ggtggcgcaa ttggaacagg attattttta
     4021 ggttcagcaa aggcaattgc atcagcaggg ccagctgtta ttctcgttta tttgatatgc
     4081 ggtttggcta tttatggttt gatgcgggcc atgggtgatt tatttttagc aaatagtaca
     4141 tttaactcta tcagtgattt cgttgactat tatttaggca aaaagtgggc attttttgtc
     4201 aattggactt attggctttg ctggcttgga gctggacttg cggaattaac agcaatagga
     4261 ttgtatatcc atttctggtt tgtacatgta ccagctttga taagcggagg tagcgcacta
     4321 atgatcttaa tggcagtcaa ccttgttgca gtcaagtggt ttggtgaatt ggaatctttt
     4381 ttctcaatca tcaagatctt ggggattttc ctttttatct tattgggtat aggattgagt
     4441 gtctcagatt ttcataccca gtctatcgga atcaaagaaa attttggaca gatcgatgaa
     4501 ttactaccgt ttggctttgc tggaatggta gtctcatttc ctatggtttt attcagtttt
     4561 gctggaattg aaatgatcgg cttaaccgtt ggcgagacaa gtgatccgca aaagaatttg
     4621 aaaaaagcaa tcaactgtct gccttggcgt attctctttt tctatatagg aacgattttg
     4681 tcgattttat ttgttgttcc atggcattgt ttagatttaa aagaaagtcc tttagtgact
     4741 atgctgcaag tgatccattg ccaaggcgga gcagcaattg tcaatctaat gattttggtt
     4801 gcttctttgt cctcagcaaa tagtgcagtc tacagtacta gcaggatttt atatcaaaca
     4861 gcaaaagaaa aaagtttgcc ccattggttt tgtgcgcttt ctaagaaagg cgtaccttta
     4921 cacggtattt taattacagg aatgttattt tttgccggtc tttgtctaca tttttttgta
     4981 gctgatagtc gattgttttt tcagcttttg gcaggcatga caacgagttg ttttctattt
     5041 gtgtggtctt cgatcttgtg tgcacatgtt caatttatta aagaaaagaa acggaacact
     5101 acacaaagat ataaagatct tgctttgttg gtgtttttta cttcaattgc tttcagcttg
     5161 ttattcgaaa gcacgacacg ctttattcct tttttattgg ttatctggtt tgtattctta
     5221 aattttgtat ataaaagaat aagcaataga aatatcagtt aggcccagat acattttgca
     5281 aaacgaatta tttcagaccc ccctgaaata atcgatacgc ctgaaaaaac ttccctgcgt
     5341 ataataacag tatcaattaa acaggagggg ataggatgaa ctggtacaga ttgacggtag
     5401 aagatgtatt gaaaaactat caaacatcaa cagaaggctt gtcgcaagaa gaacgacaga
     5461 aacgcctgac tgatcaagga tataatgaaa taaaagcaag gcagcagaca aaacgatgga
     5521 aaaaaatagc aaagcatttt actgatctgc tgatgatcgt gttgatcatt gcctcttttt
     5581 tgaaatttgc ttctagtgaa tatattgaag gaagtattat tttattcgta gtgatcgtca
     5641 atggactcgt tagttactgg caagaaagaa aagcggaaga atcattgaat ggtttgaagc
     5701 agctgatggg acaagaagcg atagtactat caaacgggat gcaagaaaaa atccctgcga
     5761 aagaactggt aaaaggagat gtcattgtct tacgtgccgg agatgtcgtt ccagcagata
     5821 tccgattaat cgaagcacat gatttattag tggaagaatc gattttgacg ggagaatcag
     5881 aagcatctga aaaaagtcat gccgttttga cagaagaaga atcgatcggc gaccagaaaa
     5941 atatgggttt ttcaggaacg cttgtacaat ctggttctgc agtcggtgtc gttgtagaaa
     6001 caggaaacac gacagaaatc ggtaaaatca atcaagcatt gcaatcggtc gaacaacaaa
     6061 cgacgcctct gattaaaaaa atcaaccgat taaacaaaca aatttttcaa ggcattcttt
     6121 gtttgagcct attcttagtt atttttacga catttcgata tggtatggat tggcatattc
     6181 ttctttcggc tacgattgct ttggtggtgt caatggtacc cgaaggattg ccagctgttt
     6241 tgaccatgat tctatccgtt ggagtacatg aaatggcaaa agaaaaggcg atcatcaaag
     6301 gacttccttc ggtagaaaca ttaggttcta tgacagtgat ctgttcagat aagacaggaa
     6361 cattgacaaa aaatgaaatg acagttgtgg atgtagctgc aaaagaagaa gcctgcgtgt
     6421 tgtccattat gaaaaactgt caggaattaa aaacaaaaga agaacagaaa acagaaaatt
     6481 taagtggcaa tccgacagaa gttgcgctga ttcgacacac agaaacagcc aatctacctt
     6541 tgaaaaaaat agaagcaaag attccgttca gttctgaata caaatatatg gcaacgatgc
     6601 atgcggagga aaaaggagcg gtgatttacg tcaaaggtgc accagaagta ttatttgcta
     6661 aatcaactct ttcttctgca gaacaagaag catggagtca gacagctgca gaatttgcga
     6721 gaaaaggtca gcgtgtttta ggatttgctt ataaaaaagt agattcgaaa caggaactca
     6781 cgcatgagac tttaacccaa ttaacttttg cagggatcgc cggattgatc gatccgccaa
     6841 aagaaagtgc agtcaaagct gtaaaggaat gccaacaagc agggatctca gtgaaaatga
     6901 ttacagggga tcacaaagac acagcaaaag cgattgcgga acaaattggc ttaaaacata
     6961 ctgccaaagt tttagaaggg atcgatttgg atcttatgtc tgatgaagag ttgattcagc
     7021 aagtgccaat agtcgatgtc tttgctcgaa cgacaccaga acataaactg cgaattgtta
     7081 aagctttgca gaaaaatgga gaaatcgtcg gaatgaccgg agacggcgta aatgatgcac
     7141 ctgctttgaa aagatccgat gtaggaattg ctatgggaat caaaggtagt gaagtaagca
     7201 aacaggcagc tgatatggtt ttaggagatg ataatttcca tacgattgca aaagccgtaa
     7261 aagagggccg gcggattttg gataatttgc aaaaaaccat caatttcttc ttgccaactg
     7321 cattagctca aggattgatc ttgatttggg cacttatgct gaatcgcccg ctacctttgt
     7381 ccccagtaca gattttatgg gtcaatatgg taacgacaat cactttatct tatgcactag
     7441 ggttcgagtc gccagatcca gaaatcatga agcgcccacc aagagatcca aagcaaggaa
     7501 tcctatctgg ttatcatttg tttcggatca tctatgtctc cctactgatc atggttcctg
     7561 cctatttatt ggctatgcag tttgaagggc aaagcttaca gcaaaccatt cttctgcaaa
     7621 atatcgtctt agctcaagct gtttacatga tcaattgcag agagttgtcc aagccgtcat
     7681 tgaaccaagg tcttttgcaa aacaaagcgc tttttctttc attaggtatc cttgctttat
     7741 tgcaagggat ggttattttt ctaccagtcg gccagcagct gatcggtaca gtaagtttaa
     7801 cgatgacaca acagttattg attggagcaa atgtgattct tctattctta gttgtagaaa
     7861 tagaaaaagg gatcatgaac aaactgtttc gcaaaaaaga aaaaacagct agagaataca
     7921 gtcgctagcc gttactataa aaaagttttg aaagtgagtt cagtcaaacg attgagctca
     7981 tttttcgttg gaatagaaga aatgtataca atagggatat ctagagaatc tagagggaaa
     8041 ttggaaatca agatatctgt tttttcaaaa gaaatagtgg aaagatcggt tagttcgata
     8101 ggtaagaagc agacacgttt tcctaagtaa tcttttaatt cctgctgcaa gaacgccttc
     8161 catgaaggtt ctccttggaa aataaaatag acattgaaca aattcggttt gatagaaaaa
     8221 atcgtttgtt ggatcagtag agacaagagt tggagaaagt aatttggttc attgatttct
     8281 atttcttttt ttagatcgtt cagtaaattc tctttctgtt gatacaagtg tgcatattgc
     8341 tgatcatatg tttctaaaaa acgatggtat tgacggtcca atgcttcaat caatatcggc
     8401 gattcatgat attttaatat gaggagagcc aagttatcag tcagagatgt tttttttagt
     8461 tgatcaagag acagctctga agctagttcc tctacaaacg tttcgaggac ctctaaaaat
     8521 ggagaatgat gcaggcagcg agcggtttct tgtccatagt tattgttgta attccaactt
     8581 tccagaaagc agaaagaagc aaaagaaagc tcagtgtctt taagataaat accttccgtt
     8641 aaatacaatt ttttcaatgc agagtcatac agggaggaga gtgtgctgtt ggtatggata
     8701 gaagaatcta atcggcaatt cgcttttata cggatcgtat tgataaagaa ccaacatgtt
     8761 ccgaaaatct cttctgtttc aaccgctagt tcagtttgac tgaggtttct taaaaattga
     8821 aaataatggg attcatggat cgaataatcc tcgaaaaaat agttgttatg agtgaaagga
     8881 atcagcaatc gatgataaaa agtacgaatc gcacgctctt ttccaagaat acaagctgta
     8941 tatttcgtca aatcgatctg caagtcgtaa cttgctaaac gctgattgat tttagtcaca
     9001 tgtcttttca atgtcccata agaaagccca tggtcggcta gaaaatcagg gacagcaact
     9061 ttttttgtaa aaagcagatt tttcagcagt tcgacactga ctgattgttt gaaaaaaatc
     9121 tcccaaaggt catttgttaa taaattttgc tggttttgca aactgattcc tgcttgatcg
     9181 ctgatgatgt cccagccttc gggcagctga gagcgaatag attgaaggtc agaaaaaatc
     9241 gttcgttcgg ttgtccctat gatttcagct aattcattta cggttatctt tgtatgattc
     9301 agcaattgtt ctactaaaga aacatgacgt cgaatatctt tttcggtaat gatttttttc
     9361 attagtgaat acatcaatca gactcccttc tattctaaga atacggagaa aataaacggt
     9421 ggtcaagttt caactgattt ttcaatcatt gtttattttt ctggagcatt tactgatgat
     9481 aagaatttca tcatgaactt gaaaaatgat accactaaaa tcaaaaaata ggtgggcaaa
     9541 caataataaa cattttaata gtagatggta gcaagaccaa cacaggcgga ttgaacattt
     9601 aagagttgat aaaaaagaaa aatatcgaag tatttgatta gcaactactt gtattcattt
     9661 ttctattctt acgtggtatc tttgaataaa gcgaatcaat aagaaagaga tggaagcaga
     9721 tgctaacgaa aaaagaacta atcgatcaca tcaatgaaac atacaatttg tctccagaat
     9781 atatttttaa aaaatttcct gattattgcg tattcagaca tcaaaaaagc aaaaaatggt
     9841 ttggggtcct gatgtctgtt ccagaagaaa aaatctatga tgatgcaagt gaccatagga
     9901 tcatcaatat tcttgatttg aaggtcgatc ctgaacttgg cggaattttg cgaggaaaag
     9961 aggggtattt tccagcctat cacatgaata aagaacattg gatctctatt gatctacgta
    10021 aaatagaaaa gctagatgaa ctagcagatt tgattgatgg aagttttaaa tcaacgcaat
    10081 gaatgtctag gacaatagag aagaggttgt gacagaaatg tttagcttca agaaataaga
    10141 aggaatttac gaaaattgct tctcaaattt ttgggaaa
//
LOCUS       DAZUMV010000096        10155 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000096 DAZUMV010000000
VERSION     DAZUMV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.097"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..475
                     /locus_tag="ACG8FK_002075"
     CDS             2..475
                     /locus_tag="ACG8FK_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS5097665.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            484..711
                     /locus_tag="ACG8FK_002076"
     CDS             484..711
                     /locus_tag="ACG8FK_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5097666.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1347..1718
                     /locus_tag="ACG8FK_002077"
     CDS             1347..1718
                     /locus_tag="ACG8FK_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097667.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1974..2216)
                     /locus_tag="ACG8FK_002078"
     CDS             complement(1974..2216)
                     /locus_tag="ACG8FK_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5097668.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2248..3150)
                     /locus_tag="ACG8FK_002079"
     CDS             complement(2248..3150)
                     /locus_tag="ACG8FK_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5097669.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3163..3351)
                     /locus_tag="ACG8FK_002080"
     CDS             complement(3163..3351)
                     /locus_tag="ACG8FK_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5097670.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3365..3928)
                     /gene="amaP"
                     /locus_tag="ACG8FK_002081"
     CDS             complement(3365..3928)
                     /gene="amaP"
                     /locus_tag="ACG8FK_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5097671.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3966..4859)
                     /locus_tag="ACG8FK_002082"
     CDS             complement(3966..4859)
                     /locus_tag="ACG8FK_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5097672.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4937..5860)
                     /locus_tag="ACG8FK_002083"
     CDS             complement(4937..5860)
                     /locus_tag="ACG8FK_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5097673.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5909..6259)
                     /locus_tag="ACG8FK_002084"
     CDS             complement(5909..6259)
                     /locus_tag="ACG8FK_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5097674.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6292..7194)
                     /locus_tag="ACG8FK_002085"
     CDS             complement(6292..7194)
                     /locus_tag="ACG8FK_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5097675.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7187..8044)
                     /locus_tag="ACG8FK_002086"
     CDS             complement(7187..8044)
                     /locus_tag="ACG8FK_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5097676.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8378..9187
                     /locus_tag="ACG8FK_002087"
     CDS             8378..9187
                     /locus_tag="ACG8FK_002087"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5097677.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9227..9724
                     /locus_tag="ACG8FK_002088"
     CDS             9227..9724
                     /locus_tag="ACG8FK_002088"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS5097678.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         2924 a         1929 c         1751 g         3551 t
ORIGIN      
        1 aatgaaattt aaaacattgc tattaaaatt tgtcgtttta ggtataagtg ccgtatttat
       61 tttgttcggc gtactttttt atactcagat cgctacaagc gaaaaaggat tcaattttga
      121 ttggcagacc acattatttg tttctgtcat ttactttgcc cttctcctag ggatcgttat
      181 tgcttatttc ttattgaaac tattgaaaaa aatcgaagat aaccaagctt tttccactca
      241 aagcttaaac atactaaaaa aaatcgaacg aaccattttg tttattggtt tcgccttttt
      301 tggcttactt cctaaattta tgaaagtagc ggatttagac gatgctccag gtttgatgtt
      361 ggtaggaatc gctgtcgtat tcattccttt tgcagtatac acattaacaa gcgtgctgga
      421 aaaactgctt gaacatgcga ttttacttaa gacagatcat gatttgacag tgtgaggtgc
      481 gacatggcaa ttatcgttaa tttagatgta gtattagctc aaaaaaaaat gagtgtgact
      541 gaattgagtg agaaagtggg cattacgatg gcaaacatct ctattctaaa aaatggaaaa
      601 gcaaaagcca tccgtttttc cactctcgaa aaaatctgcc aagttttgga gtgtcaacca
      661 ggtgatttgt tgacctataa aaatgactcg aatactatac cagaagaata aaataaagta
      721 gaacaaacag taaataaaaa ataatctgaa tgatagatgt gtgagacttt tagaagttat
      781 ttacgtctat tattcagatt ttttatttcg attgaatacc gttttatcta taaataagat
      841 atgtactgac tcatacaaag ggggcctttt cttctccatg tactatcact tttcctgata
      901 caactggttt cctgttacgt tgtagttgga tctgctttta gtataatact ttattcattc
      961 caccagactg atagcagttt gagcagtcta aataatcatg tagtattaca ggccattctg
     1021 tttcatacct tgaagtgctt aaaacttcag ctaatggccc attgtgttaa ctcttactcc
     1081 attctttttt agtaagctcc catatatact gtaaagcctt ctatatatta tgtcatgttg
     1141 ctttttaaca tttactatgt agttttgata aaatgatttg aataacagtg atggacaatt
     1201 cttttttatt tttaacatta ttttgataat aatgattatt tatttaaaaa tacattagat
     1261 aacatttgtt acgacacgaa aaagcttcac aaacaaatat cgttatttta aaatgagggc
     1321 gaactcacac aattaggagg aatagtatgc catttattgt ttactttttt ctttcattac
     1381 tcgtgatcta tattccatta ccaacatttt tcctgctcgt caatcgtcta gcagagcaac
     1441 atgatacgat tgctgctctt acgaaacttt gtttacttct atttgtttgt ctagattact
     1501 ttacgagcta ttattggcta aaacagacaa gagtaaaaaa gagatattat ttcttccttg
     1561 tctttttcaa tctctttgag tttctgatcc attgcttttt acttcatcag tatcatacga
     1621 tccatttgtg gatcatctgg gttttcaaag tggtcgtatt attttgtatg ttcacctttc
     1681 ctatctcaag aaaatttagg gaggcattgt tttattaaag aagcttctct gtatcaataa
     1741 agacacattt aaggaaaaac ttttaaaaat ctaattggga atttgcccgc ataaataaga
     1801 ggctgtgaca aaagtcttgt cacagcctct tattccgtat aaacagtgtt caaaagcttg
     1861 catcttcgat aatatgccca accaaatgaa aaatatgaga agctattttt gtttggctgt
     1921 tcttattact tgatgaagaa cagctttttc acgacctctt tttaaaagtc tttttacagt
     1981 tttcgagcaa agaatgatac gattagtact aaaatgatgg caccaatcaa tgatggcacg
     2041 attgccattc ctgcaatact tggtccccaa gaacctaata atgcttgacc aagtgctgat
     2101 ccaactaaac ctgctacgac atttgcaata atccccatcg aagaaccttt attcgtgatt
     2161 gctcctgcaa ttgctccgat aattcctccg acgataagtg accaaataaa tcccatgttt
     2221 cgttcctcct aatgttttac ttccttatta ctgaacccgt ggttctgtat tttcttttac
     2281 tttttgtgcg ccgcttccag ctgctgattt tactttatct gtttgattcg aagcaaattc
     2341 accggttttt ttcgctgcgt cagttacttt gtcttgcacc gtttcttgat ctttttcaaa
     2401 ttcttctttt gttttgatgt ccactacgtt agcgttgact tcgatcactt ctaaatgtgt
     2461 catatttttt acttctttgc cgattacttc ttgcatttct tcaaagattt tttcgatatc
     2521 tttgccatat tccacaatga catccatatc aactgctact tgttttttac cgacttctgt
     2581 ttcgatccca gccgttacat tatctgtatt cacaagtttt cctgccacat tagagaagaa
     2641 accaccgttt gcagataata aaccgtctac ttgttcaatt gcgattccga tgattttttg
     2701 cacaacttta tcttcataat tcagctcacc gcgcattgaa tcttcctgtt cagaatcatg
     2761 ctttccttgg ttatttgtat gatcagaacc aagttttcct tgtccgtttg tgtggtcaga
     2821 actaagtttt tcttgtgctt tcgcatggtc agaaggatgt ttttgctctt ccacaggttt
     2881 agaaggatcg attgcatcat gttgttctgg aactttagat tctggagttc ctggatgatg
     2941 aggtgcatgc ttttctgcat catgtttctt ctcttctact ccttgttgaa cgttagggac
     3001 tactggatct ggatgtgcag atactggtgc tgttttgttc ttttcatcgt tcacaccatt
     3061 tgattggttt ggtgtcgttg ggtctggatg tgcagacact ggtttttggt tgttcatttt
     3121 attgttatta tttttgttat tgttattcat ggatgtttcc tcctaagatt taaaatagtg
     3181 atctaagatt ttcgtttgtt tcagataaaa accgatagcg acgccgatcg ctgccatgat
     3241 caagatcaat aaggtcttga aaaagcccac ggtgatccat aaaacggcta atatcaatcc
     3301 aagtacccca aagatgatag ggagcttgta tgtcgaaaat agttctttca tttttggtcc
     3361 ccccctattc tactcgtttg cggtcattgc ttcgtttagc tggttcttca aagcctgtgt
     3421 atttcaccga tactttcact ggctcttttg ttcccaatat gtgttgtact tcattttcga
     3481 tttctttcat caatgttcct gttttaccaa tgagtgctga agttcgtttc aactcccctt
     3541 ttacattgac ggcgatcttg tttttcgttg ctttgacatt tacttttggt gaatggatga
     3601 attcatcttc atgcaagtga gaacgaacca ttccttcaat tgctttttta tccaacgtca
     3661 actccccatc accatgtttc agtagaaagt ttccgattgt ttttggataa aacaggaaaa
     3721 caagtaacgt gataattgtc aaaatagcaa atgcaacggc aagccaaaat acatattgtt
     3781 gcacgtatga attagtgatg gaaaagaatc gaaacttctc tagctgaaaa ggcaatggat
     3841 aggttgcact taatagtatc gcaaacaaaa gcacagatag caaaagtaat gttccgatca
     3901 ctccgatcgt ttttacacct ttattcattt tcgcacctcc tcataggggt gaaacgatca
     3961 acttcttata agttgatcgc ttctccacca gttactccgt aaatttgggc tgtaacataa
     4021 cttgctttat tcgatgctaa gaaaacataa actggtgcta attctcctgg ctgacctgca
     4081 cgttctagta aactttgctg accaaattca gggatcgaat cagaaggttg tccttcgtct
     4141 agttgtaatg gtgtccagat tggaccaggt gctacaccat ttacacggat tcctttaggt
     4201 gcaagctgtt ttgctaaacc gatcgtaaag ttagcgattg aagcttttgt tgctgcgtaa
     4261 tctaataaat gttcgcttgg attgaatgct tgaacagaag ttgttgttac gattgctcct
     4321 cctgctggga tatgcggaac agcttctttc actaaagcaa acatagcgat gatgtttatc
     4381 ataaatgtgt ctttgacttg ctcaagtggt aactcagcaa tagactgaca agaaatttgc
     4441 tgtcctgcgt ttagtaccat cgtatctagc ccgccaagtt cactcacggc tttatctatg
     4501 atttctttag gtgcttgttc gtcacgaaga tcaaaaggta gtaagacaat cttacgtcct
     4561 gcatctttca cgtattttgc tacttcctct gcatcttttt cttctcctgg gaagaattga
     4621 agagcgatat ctgccccttc tctagcaaat gcaatcgctg ctgcacgacc gatccctgaa
     4681 tctccaccag tgattagtac acggcggttt tctagttgat tattgccttc ataggattct
     4741 tctccacaat caggaacagg aatcatattc tgctgtaaag ctggtgtatc ctgatcttgt
     4801 ttaggaaatt tctccgtatg gtacaagttt cgcgggtcga ttaattttgg ttctgtcatt
     4861 gctgtattcc tcctagtttt taaattgtat tgccaaaaca aatttctttg tttgatacct
     4921 gttttaaata gattctttaa tttgtataat ctttccggta taaatggtaa cctaaaacta
     4981 ctgctgctat caaagagcca aagactacga gtataaagcc tagagcagaa cttttacccg
     5041 gtaatcctcc tgtattcatt ccccaaatcc ctgcgaacaa cgccggaacg gaaatgatta
     5101 atgcagctga atctagatat ttcatgatat gatttagatt attgtccatc atcccgttga
     5161 atagatcacc aatactctct aataaatctc gatagatctc gatcattttt tctgtttgtc
     5221 gctgtctcaa atggacatca tagagtagct gttcatcgtc taaacgtttg atgaaagttt
     5281 cagaattcca taattgatcc attgtttccc gttgatctct taaggtatga tcgatatata
     5341 cgatgtctct ttcaagatct gcctgtttat acagttcttc attttccgtt gttttacgag
     5401 ctgctttatc caaagcgtca attcgctttt tcatctctct tagttctttc acgtaatggg
     5461 tgtaaatatc taataatgta tacgtgatga tcttttcaaa cgagtttaaa tgatttccat
     5521 gctttgtcag cagccgatcc acaaattcgg tttttctatc gatacaagta atcaagagat
     5581 tatcggaaag tacaaaagat attggcgtta attttttttc gatacgatta tctgactgat
     5641 ctagattaat cactaccaaa gaaaatggat gctctagttt cgtctcataa aggcgctcca
     5701 tccgagaaac ttcttccgga tattcggttc ctacgaaaat atcatcagga agctcgaatt
     5761 cttcgattgc ttgctcaatc tcttcgggct ttgcactatc aagtacgagc caattatatt
     5821 ttttttcatc tgtttctatt acattctttt cttcatccac aaagtagtat ctcaatgtat
     5881 acctcctttt tggactttct cgaaactatt agctgaagaa gcagttcttt aatttgatat
     5941 tataatcgag tactgcgatt tcattttcta atgaaaagct gtcaagctgt ttgttgaagt
     6001 ccatgttcaa tcgtttaccg aaataatttt gctctaattt agatgtatag atgtccaata
     6061 atggatgctc gattttagtc gcatattctc caaattccga aacaaaagga atgatttgaa
     6121 aaaagctgcc ttttgcaaaa gcacctttgt cccttagaaa tggatcggaa tcattgataa
     6181 gcgcgagtgc gatttttgta ttttccccaa ccttttcaat taacgtctct actgcatttt
     6241 tagacattgt aatttccatt cctactcccc ctcttcagtt gttgtcttct attatttaac
     6301 aactacatct gtttttggtt gaacttttgc atattttgga tatttaggga atttagctac
     6361 acaacggaaa gccgtcatcc ctgcatattt ttcgtaatat ttcttcgttg gcccttcaaa
     6421 atccttgctc cacggtttca catgtcctga aaaatggata atgtaaggat tgttgcgagt
     6481 ttcttcgtat tcacgttcac ctgctgctgt tggatgtttt tttgcttttg ccatgatata
     6541 gccttgtgca ttccaacgcg gatgaagtgg taaccaacgg tcatgtaaga tagcatttaa
     6601 tgcatcttgg tcatggaatc gtaatttttc tggattatgt tcgatgaaat ctaatacttt
     6661 ttgcgtaata ttttcatcta accatttttt cacattgatc agcatcaaac ctgagttgaa
     6721 ataacgcatt gatttagctg aaattttcat tttttccagt cgttgatgga acccagcgtc
     6781 ttcaacagct gctacgattg aatcaccgaa gtcaagacgc cataatttag agatatcttg
     6841 taaagcgatc atgtcgcaat ccatatataa gatacgttca acttctgtgc cgcggaacaa
     6901 ttccggaatc gcaatacgat aataagctgt ttctggaata cgatcgctga ttaccacatt
     6961 tgtaaagaat tctttattga ttttcaagaa ttccacatct gagttcattc gtgcgttttt
     7021 cactgagaag cgtaaaagct gtttactttc aaaatcgata tcatcgtcaa tcacatagaa
     7081 ccgaacaaat ttagacgggt tgcaattatc taatactgag acaaataatg cagctaaatg
     7141 tggtacgaat tttgtgttac aacttgcgac tactgctatt tcttttttat tcattgatct
     7201 cgcctacttt cgttagagta ctgtgtgcta atttttttaa gtataaattg gtatacggat
     7261 gatctttcaa cgtattccaa ggtttatcat gtcctgtaaa gtgaacgatc gaaggttttt
     7321 catttccttc tttatactga cgttcatatt tttcatttgg tgctggatga cgttcaaaaa
     7381 tcagcgaagt ctgcatgttc cattttggat gaagttgttc ccaatcttca taaagtacag
     7441 cattcaaagc atcttggtcg tgatagatga ttcgatcgcc attttcactg aggtaatgga
     7501 tagttttttc tgtaatttct tttttattcc attgatcaat gtcaatcacc atcactcctg
     7561 aattgaagta caagtcatcg gaatcgattc ctaatctttc caaagctttc acttggcctg
     7621 gatcgataat agcgccgatt gtttttccat ttaacggctc atcatatagt tttacgatat
     7681 cgtctagtac taacacatct gaatctaagt aaaggacttt cttataatcc tctttagcta
     7741 ataagtttgg taaagaaatt cttaagtaag cggtagttgt gatatggtca ctgactagga
     7801 agtcttcgta gatatctttt tccactgtca ggaattggat acttgcatta cttgaatatt
     7861 tatttaccgt ttcttccaat ccttctttac tatattctga tagaccatcg tcaatcacat
     7921 aaaattttat gcgtcttgtt ttattgcaat tttctaatgc agttgcgatc atcacgctca
     7981 agtaaggagc ataattttcg tctgaagcag ttaccacagg aactacgccg tatctcttct
     8041 ccatcaaaat cactcctttt tctaatcttt ttctaacctg ttctacatct agaatattcc
     8101 tcatttggtt ttaaagcaaa atatatgctt tgttttcatt gaatatttta aaattttttt
     8161 atcacaaaac gcatcggtaa gcgctaacta ttcgtttttc atggcttttt attcaaaggt
     8221 attgaccaaa aactactata tatagccacc tttttcttaa gaatgttttt tgaaaaaatt
     8281 cataatgaca aagcaagtta gtttcatttt tttaaattct catttaatct taaggtaaca
     8341 ggaaaataga tatgtaaaaa gaaagtagga agataaaatg aaactagcag cgattgattt
     8401 agacggtacc ttgcttgatc gaaatggaaa gataccagaa gaaaatatcc aagcacttcg
     8461 gaaatttgat gaaaatggcg gaatcgtaac cgtcgctacc ggaaggaaca gcatttctgc
     8521 taaagatatt ttttccgaat taggaatcgg tggctatttg atttcttcca atggttcact
     8581 gattgctgaa atgaaggatg gaaaaatggc gaaagttctc agacgttcaa aaatagatag
     8641 cgatacactg agaaaagcct tcttctatgc tcaagaagcg aaaatatcca tcattgctag
     8701 ccgagaaacc caagatgatc agctgacatt cgatgaatca aatctagtca aagatgaccc
     8761 ttattatgaa catttcaatt tacagaacca ttcttttgat caaattttaa atatgttgga
     8821 agatccttct cttagctatc ttaaattagc attaacagat aagagtgaag aaaaactaca
     8881 gaagctacaa agagatctgc aagcagatgg tatccaatca gtattctctg atccgcattt
     8941 cttagaaatc acgcctacag gtatcactaa agctcattca cttgtttttc tggcggatta
     9001 ccttggaatc gaatcgagtg acatcatggc ttttggcgat caagaaaacg acattgctat
     9061 gttgaattac agcgggttga gtattgcgat ggggaatgca cagcgacaag taaaagaaat
     9121 tgcaaaccaa gtaacactga cgaatgaaga agctggtgtt gcttatattt taaaaaactt
     9181 ctattaaaaa gaataaaatg acgatatgaa tgtaggagga aagaaaatgt tagttattat
     9241 tgatatgcaa aatcatattc ttgatccaaa cagcgaattt tatattccag agtcagatca
     9301 attggcagaa cgtattgctc aaagattagc taaagcacgc caaaataatg aatatattct
     9361 cttcacacgg gatataccag ttgaactgaa ggacaaagaa gaagaacgag aagatttgca
     9421 attgatccct cttctttctc cacaagaaaa tgaacgagtg attaaaaagt attactttac
     9481 gattccacct gaaactttag ctgaaatcca ggaaacttta tttgaaagca aagaagaaca
     9541 aaaagagatc gaagtagtcg ggattgaaac aaacttgtgt gtactctcta atctgatagg
     9601 cctgcagagt gcttttcctg aagcagactt ttttgttgat ccaatgttag tctccagtag
     9661 aaaacacgga gaatccgctt tagagctgtt gaaagatttc aacgtctcta tcgtcgaatc
     9721 ataaaaatat ctatcttcat ttacgatatt taacacacaa taaaaaaagc acgcagaact
     9781 gttttagtcc tacgtgcttt ttctatcaaa gtagattggc ttttttatga cacagagtga
     9841 aacggcaggc tcacgacctc tttcttaaat ggtgttcaat cagctgaccc tcggtattag
     9901 cttaacaaac ggcaaaatat gaggaactat ttttccgttt gttaagctaa tactcaggtc
     9961 aaaacgctga tttcacaacc tcttccttaa acggtattca aaagcttgca tctgcagtaa
    10021 taagaacagc caagtaaaaa cataagaagc tgtttttact tggctgttct tattacttga
    10081 tgcagggcag ctttttcaca acctcttatg taccttgtag gactcgaacc tacgaccgga
    10141 cggttatgag ccgtc
//
LOCUS       DAZUMV010000097        10004 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000097 DAZUMV010000000
VERSION     DAZUMV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.098"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..469
                     /locus_tag="ACG8FK_002089"
     CDS             <1..469
                     /locus_tag="ACG8FK_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HFS5097679.1"
                     /translation="PIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQE
                     QRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVY
                     PLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            504..1271
                     /locus_tag="ACG8FK_002090"
     CDS             504..1271
                     /locus_tag="ACG8FK_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5097680.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1463..1861
                     /locus_tag="ACG8FK_002091"
     CDS             1463..1861
                     /locus_tag="ACG8FK_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS5097681.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2168..2542)
                     /locus_tag="ACG8FK_002092"
     CDS             complement(2168..2542)
                     /locus_tag="ACG8FK_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097682.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2564..2914)
                     /locus_tag="ACG8FK_002093"
     CDS             complement(2564..2914)
                     /locus_tag="ACG8FK_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097683.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            complement(2931..3326)
                     /locus_tag="ACG8FK_002094"
     CDS             complement(2931..3326)
                     /locus_tag="ACG8FK_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS5097684.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(3393..3578)
                     /locus_tag="ACG8FK_002095"
     CDS             complement(3393..3578)
                     /locus_tag="ACG8FK_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFS5097685.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(3660..3914)
                     /locus_tag="ACG8FK_002096"
     CDS             complement(3660..3914)
                     /locus_tag="ACG8FK_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097686.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(3936..4181)
                     /locus_tag="ACG8FK_002097"
     CDS             complement(3936..4181)
                     /locus_tag="ACG8FK_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097687.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(4188..4472)
                     /locus_tag="ACG8FK_002098"
     CDS             complement(4188..4472)
                     /locus_tag="ACG8FK_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097688.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(4966..5451)
                     /locus_tag="ACG8FK_002099"
     CDS             complement(4966..5451)
                     /locus_tag="ACG8FK_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097689.1"
                     /translation="MGWYGAYSSANEVIEEKKGYAKNGLEIVDTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKQQLDRIKEE
                     NNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     A"
     gene            complement(5618..5884)
                     /locus_tag="ACG8FK_002100"
     CDS             complement(5618..5884)
                     /locus_tag="ACG8FK_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5097690.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(5886..6107)
                     /gene="relB"
                     /locus_tag="ACG8FK_002101"
     CDS             complement(5886..6107)
                     /gene="relB"
                     /locus_tag="ACG8FK_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS5097691.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            6333..7151
                     /locus_tag="ACG8FK_002102"
     CDS             6333..7151
                     /locus_tag="ACG8FK_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5097692.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            7164..7451
                     /locus_tag="ACG8FK_002103"
     CDS             7164..7451
                     /locus_tag="ACG8FK_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097693.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(7633..7986)
                     /locus_tag="ACG8FK_002104"
     CDS             complement(7633..7986)
                     /locus_tag="ACG8FK_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097694.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(8006..9061)
                     /locus_tag="ACG8FK_002105"
     CDS             complement(8006..9061)
                     /locus_tag="ACG8FK_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097695.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            9277..9675
                     /locus_tag="ACG8FK_002106"
     CDS             9277..9675
                     /locus_tag="ACG8FK_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097696.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            9678..9851
                     /locus_tag="ACG8FK_002107"
     CDS             9678..9851
                     /locus_tag="ACG8FK_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097697.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         3279 a         1573 c         1739 g         3413 t
ORIGIN      
        1 accgattgcc caactgaata aatacactga ttttaaaatt gactatgagc ttatcaagaa
       61 gggtcatact tatacaaaca taaagttctt gcttacacgt accatgtcag ttccggtacc
      121 ttataagcaa gaacaaagag accctgctta tttaaaaggc gcagaagata aaacagagaa
      181 aatgaatcaa tcgtacttga aagctatgaa tagcaaatac actaaattac taatcaaaaa
      241 ctttttgatc agttctgccg acttatgcga tcttaaactt atgagccgac ttcagaataa
      301 agtttatccg ctttatgatc gtttatccaa agatagagga tttgaatcag ttgtcaatca
      361 cctatcttac gttgctactc ataaggagga ttacacaata aaaaatactg ttaaatatct
      421 cgaaaaatca attacgagtt atttgcctta tgtgaacctt tatgattaac aactgaaaaa
      481 atgcgtgaag gaaggaaaaa cttttgaaaa aagatgaact gacaaagtac aatgaggagt
      541 tcttagcaac aattgacatg aaaatggatt taaagtcgta tgaactttat caattgctgt
      601 tcagttatat ctcacccaca aagctaaaga aaacaggcgt agtagattta cctgttaaag
      661 cgattattaa ttacatggat gttgatcaaa atcttgaaac ggaactaaaa accatagtta
      721 acactttgca aagacaagca gtttatgaaa tttcgaatct agacaaaaaa tataagacgt
      781 atactactgt atctgctatt gatcggattc attgggattt agaagagcat agtgtatctt
      841 tcagtttttc agaagatgta attcccttaa ttgtctcaat caaaaagaag tatccgttct
      901 ttgtttttga agacttagca tcttttcaaa aaaaatatag catcgtgtta tacaaatatt
      961 tggcgatgta ttataaaaaa tatcttatcg ccaaaaataa aggagatttt gagaaagctg
     1021 aaatatataa gaatcccctg attgaaatag ttaaaattcg tgagattacg aaaacagaaa
     1081 atgagtataa aacaatatcg aattttgata gacgtgtttt aagggaatca gttgaggaaa
     1141 ttaatgaaaa atcaatgctg aatattgatt ttgctaaagt tagaaaaggc agaaattaca
     1201 attttgctgt ccagtttaat atttctagtg agtttgatga aaaaacttat agagaaaaaa
     1261 aattaggtta atgcaaaata tatcttactc gtttgaagat gcttcagacg tatgctaaga
     1321 tatatagtgc cttgggagca aggtattaat ctcctttaga ggttaattag cttgtgatcg
     1381 cctatcaatt tttttggtag gcgattcttt tttttgaaac aaaaaagcaa aaattatttt
     1441 ttaattagag aggtgcaatg cgatgaaata ctttttttgt gcagtattcg aatggaaagg
     1501 aaatgaatat gcagttaaat tcccagattt tccagactta tatacacatg gtgaaacttt
     1561 agaagatgct tttgagatgg cgcaagaagt attggaagga tacttggtgc ttttagagtt
     1621 gcaagaaaaa gaaattccat cgcctacgcc tgttcaagag ttaaatgcaa gaatcaagcg
     1681 atctgagaat gttaatttat ttgtcgtaaa agctaatacg aaagttgcta aagctcgaaa
     1741 caagcaaaat tatgatcgaa aatcagtgac tgtggaacac tatttaaatg ttttagcggc
     1801 tgaagagaat attaatctgt cgaaagcttt gaatgatttg ttgagagaaa agttgatttg
     1861 atgtctctca gatgatcata ttaagcaaaa acagcttgtt ttaggacgta tggatttaag
     1921 aaagacaaat tatcaaagaa tatattaaaa gctctcagaa gccttctgag agcttttaaa
     1981 ttgctatgta ttgtactgca tctgctcaaa ttgattttct tttcatgctt accatgtatc
     2041 taattttcca tcaagtaatt tttagatatg tattgagtaa taaatagcaa atttgcttga
     2101 tataagctcg aattaggtgc tttgtaatgt gttaggataa aattatacaa gaaaatgtca
     2161 aaagctctca gaacgcttct gagagctttt tatcggtagg cttgtttaac gtgatcttga
     2221 aggataccct tttgatgttt ctctcgatcc ctgcatcgct aattactaag tgttcttcaa
     2281 gtgtgatgta ggtatataaa ttaagcatca aaatggtgta gataagcaaa aaactcccta
     2341 aaactaagtg ttgattataa aaatctgact tataaaaaag aactgagaat gttagtgttg
     2401 attcccaaaa aagaaacaaa attttgtatt tccaaagata gtggatcaag cgctgtgctt
     2461 tacccatctc aaataattta taataacctg ctaattgatc agcaagcgta tcgttaacgg
     2521 tttctgtttg tgtttgtttc atctttctct tctcctgttt caattaatct aaaatttcag
     2581 attgagcaag caactcattg tggtagtcgg tttgaaattc aaataacaat tttgccttct
     2641 taacgaggtt taaagcgttc gttagtgatt cacttaatgc ttcaacatct ttaactgatt
     2701 ttgcatgttt cattttttca gtgtgcgtat agcagaattc aggtattgag ttaggggttg
     2761 cctgttcaat aaacatctca attcgcaagt tattatcctc aaaaataatt agatcattgg
     2821 caaagtgttt ggtactaacg ctaatgccag ttgtatcttt taatagatta ttgattaacg
     2881 ttcgagagaa atagtttaag tgtgtagctt tcataggttc ggtctccttt ttacttttca
     2941 agcaactctt cttccagtgc tttttgaagt agttgagaga agtttatgtt tttctccttc
     3001 cctaaaagaa ctaaccattc cggaacacta acattttttc taattgtttt tgtatttaag
     3061 cttcttcgga aaagtttaat gtttagttgg atgattacta ccacttggtt ggctttcaaa
     3121 gttaattctt ccactgctga tggtttagga aattctgcat ctggtacact atatagcgct
     3181 aatcctaaag catccttagc gtttaaatat gcttcttcaa gactatttcc aaaggtagat
     3241 atttccggta agtcagggaa aacgatgtta taagcttctc cttctttctc gaagattgcc
     3301 ggataaaaaa tattttttgt cttcatttcc tcgctccttt tctagtgtaa gagaggatag
     3361 tatgcctaat accaaatagg aaggcaaggc tgttaaagcc ctgcttgctt ccagatatta
     3421 gcttctgttc ctagtggtac atcaccattg tggatcggaa caacgaccgt tcttttagtc
     3481 ttggaattat aaaattttgc atgactgcca gtttgtccag tctttttgaa gccgttagat
     3541 ttgagaattt ttacaatttc tcttgctgtc ttaggcatag ctaccctccc tttacaaaat
     3601 ctattataca cattgttgtg tatggaatca atagaaaaag aggatgtttt attgcaaaac
     3661 taataaaatg atttattggt tttttcagca aattgaatgt tattagcgtt gcaaatattc
     3721 atgatttccc aagtatcatc agaagtatag gtatctaaga attcattgac agttacggtt
     3781 gattcgtatg gttcataagt caaagcgttc tccaagtctt ttgatgaaat aatcaagact
     3841 ttttcaattt cctccccttc ttcatctatg aaaacatatt ctaaagtgat ttgttcttca
     3901 tcggtataat tcatattgtg ttctcctttc ttgtactaag aaaaatggat atttccaatt
     3961 gcatcccact cggcaccatt gtggtatagg acgtgaccga gcgttacgcc tttgaaatgt
     4021 tcttctaaat acatccaaac gcattcaagg ctagtacctg ctttgagatt gtagaagtct
     4081 gcatccaata ccttttcaga agttatcgga tcaatgcgag ttggtacttc aataaattca
     4141 gtccaaattt ctgcaaaagt cttaccttcc aaagtccaca ctccctatca attaattcca
     4201 aaagcaaata gagaaatcaa gcgaccgtca atagtcactt caacaactga aaatgccgtt
     4261 gaaacagttt taacatccac actttccaat ttccctatga aggaattttt gtgctttaga
     4321 atagctttct ttaactgtct gttatacgct tgacgactgt taaacttttt gaattctcga
     4381 tccttgatca gctgtttaac gacccctgca ttaattgtat agctttctgc aatagcaacg
     4441 tgagtaggtt gctgtaatgt agctgttagc atattgtcac tccttcctaa aagttaatta
     4501 gcttgtttac aattacatta tacacactag tgtgtataat gtaaagagag gttttttgct
     4561 gatggatcag ctgtgctaaa caaaagaaaa cgatagagta acaaaaaaag tcagcaaagt
     4621 taaaaaggag gaaacggcta gtccggttgc tttgcttgtg taagtttcgg ctttcttata
     4681 gatcaaatag gaaattatca gtgatattcc agtaaagaat aatgtactag ctgtcactaa
     4741 aagaaatagg ttaatcatac gatacactcc ttctgtaata ttggtatcag atacgtttaa
     4801 atgcgtttct gagctgtttt ggatcaatgg atgttttagc cctaccctaa aatcaaagcc
     4861 cagaaaagca tttaaaaacg ttcgtaccta atacgtttat attcatatac atatacataa
     4921 acataaacat aaacgtatac acgtttatgt ttatgtattt aagttttagg ctacttcctt
     4981 aacatctcct gctgatagtc gagtgaaccg agtgagtgat ccatcttgta gtctaaaaat
     5041 aaagctacgt ttgagtttat actgataaac agcgacatac gatccatccc agattgagta
     5101 agttttacct gcaactaata agtcatcaag gtttggttta ttgttttctt ctttaattct
     5161 atctagttgt tgcttaacca ttttttcttg atcaccgaca aagaatttca accatttttt
     5221 cggaattttc ttaaagtaag caggactgtc atttaagaag tagattggtt tgtacatttc
     5281 atctcgaaag atgaagctgt caatatcgta ggttccttct gcatctttga aaaggataat
     5341 accataggtc ttcgttactt tggtatctac gatttccaaa ccatttttgg cgtacccttt
     5401 cttttcttcg atcacttcat ttgctgatga ataagcacca taccatccca taaaaaatcg
     5461 ctccttttga aagttaatta gcttgtttac acttatatta tacacaccag tgtgtataat
     5521 aacaagaggg tattgttctt ttttaggtaa agtgggatcg taaacagtga ttttgttggg
     5581 ttaacaaaaa aacagacaag ccttatcagc ttgtctgtta cttataaact tcacttctgt
     5641 gaccaataga caatataaga attgtcactt catgctcgct aatatcagct attatacggt
     5701 aggcacctac cctatatcgc cagtagcctg atttattagc tgttagacct ttaccatgtt
     5761 ttcttggatc atcagtgcct acaagatttt tctcgatcca gtttaggata acctttgcct
     5821 gaaacttatc catttttttt agcgcttttt gagcttcacg ttcatatctt acttggtaag
     5881 tcatcttata agcctaactc ttttagcata tcttcgtggg aaatactttg atcgttttcc
     5941 tcatgctctt tcattagttc cttgtaggtg gcaaagtcta ctaacgtttc tgctgtttgc
     6001 agggaattac tacgcataaa ttcagaaatg ttatctccgt tgaattcagc aactttttca
     6061 agaaaatctt tttcttcttt agatacacga atacttatta ctggcatagt ttatcaactc
     6121 cttttcaatt acattgtact acgattgtat gacaaatgca atagttatat atatgaatat
     6181 atacataaac ataaacatat acatatatat gtttatgttg tagaatataa aagcaaatat
     6241 aaatataaac acgtttatgt atataaacat atacataaac gtgtttatag tgtataatgg
     6301 agttacaaaa ggttaataag ggaggtttaa tcatggaaaa gaagttgtca atcattactg
     6361 gaaacattga aaaaggtgga acaggtaaaa caacaacaat ctacaatcta ggtggatatg
     6421 ctgtcgaaaa tttaggtatg aaggtgctgt atattgatga agataagtcg caaaacctat
     6481 caaaacgatt tcctgatgct ttcagtaaag cgaaaagaga gaatacaatc aatcatttat
     6541 atcttcatgg atcagctgaa cctttagctg taaatgagaa catggatatt ttagtagcag
     6601 gtgaagattt aaaacaagtt gagatgcaaa tgcgtgatat gcctaacaac agaaccctac
     6661 tgtttagatg gatggttgtt aactatgaac agcttaaaga gaagtatgat ctaattctga
     6721 ttgatactca taatgatgca ggtctactag ctcaaaatgc ttgggctgta tctgatcttg
     6781 tcgtaggcat ttctgatcca tctaaagatg gttttgaagc tttgctgaag cttggtaaag
     6841 atattgatgc actagcaaaa cagttgattg atattaagac tggtgaaaca ctgatggaaa
     6901 ctaaatacgc tacgatcggt agcaggatca aacataatac taattcaagt cgtgagttcc
     6961 tagaactggt accaacgtta tctaattatc ttggatatgt acaagagaag gaattgctta
     7021 atgcaactag cttagaaggt aagaatattg ctgagttcgc taaggataag aatatctatc
     7081 gtaagaataa ggatttcttt gaaagcacgt ttacgctata caacaaaata attgatacgg
     7141 cacaagaata aggagtgatt gaagtgaaag aaaatagatt taaaaaattc gctgaagctg
     7201 aagtagaaga agttcctgaa gtggttgaag cagttgaaga acctaaacaa acacctaaag
     7261 aaacaacaag ttctttggta gctaaactaa aaagtgatag ttctgaaaag aagaaaaatt
     7321 atcaattcac gcttcaacca tcgcaaagaa agaaactatc taaaatcagc gaaaagttag
     7381 gaattcgatc agattcagct ttacttggtg aaatgataga taacttgtat gatgaagttt
     7441 ttggtgagtg atcagtttaa agctgatcac tttttttatt ggaataagcg gttaggtata
     7501 gcttaactaa atttaatata cgtgtttatg gatatatacg tatacatgtt tatatacatg
     7561 tatacgtata tgtttatgtt tatatttgta cataataaaa aggctaggta ttgtaacctc
     7621 gcctttttat ttttaaagta agtctttgtt acttggtgca ttgaaccctt tcagcattgg
     7681 cttttctttc tttgctgaat gatcggatgt aaccgttgaa ggatgctctt gttgaatagg
     7741 gttatgatca gttggtttat tctttacttc tgctactttg ctatcgtttc tatcgttgcc
     7801 tgcttcaata attgtaactt cattgttctt tacacgccga tataggttca aagctttttt
     7861 gatcgtctga gagtatttaa cgcctagtgt ttcatattcg gattcaagaa actctgctaa
     7921 aggtaactcg ctaacctttt ctctattgat tataactaca taacgctctg attttccctt
     7981 attcatgttg atctccttcg ctgttttatt gagttaaacc aaacttgtaa aagcctttta
     8041 cattagaaaa ttcactgtct tcaacaaaca aggtatgcgg aattttatct tttacaagtt
     8101 gtttgtcgat aatagaagct ccaccaccag taaagacaac tttatctaag gttgtgaagt
     8161 ttttgaatgt agctaaaact tctgcaacaa ccatattggt gtatgttttt atttctcttt
     8221 caactacttc tgaaatatca aaactttcat ttccgcttgg cttaaataga tatacgcctt
     8281 tttctttgcc ttctcggata atcttttcaa gttggaaagc attaggactg atcggaaggt
     8341 caagcatatt tacgatacga ttatataaag taaaaatgcc ggtatccttt tgttcacgat
     8401 tgttttcgtc aatttcaaaa ttttggacaa catcaaaaag tttcgtgcca ccgccaatat
     8461 caacaaaacc gatcttctga ctgatcaatg tttcatcttg tacactacca tcatcagcaa
     8521 gtaggaggtt atatagtgtt ccaatcggct gtggcagtac ccaaacggta tctacgttta
     8581 tattataagc tttaccgtca acttcaactg aatgttgtcg tgtggcttgt tgcatcacgt
     8641 atttgacgat tgtttcggta tagtcggatg aaggcagacc taaaactaga ttagcggtta
     8701 aaacgtcatc agattgatct tctaaacgtg ctaggttagc taaagagaaa gataggatat
     8761 tttggtaggt ttcctgcttg tatctgctga tcccaaaacc tagtgattct atcacgtagt
     8821 catcttttcc tagctcgtgt atatccttcc caaagtaata gctttcagta gtgtttacta
     8881 ggctatattc agaaacgttt agtgatcgtt ttaatgagga acctatatta gaaaaacgtc
     8941 ctgaattaat gatatttttg ttcaaaatac gagagggaag tgagagtgga gaaacgtgtg
     9001 tactcatttc ttttactgat ttattcccta agtcaacgga aaacaataat ggttctttca
     9061 tggtatgacc tctttctgtt tgtgtttatg gtatttcggt acgaaaatag tttacaccta
     9121 tttactatta gttgcaactg tttataagaa aaaaatgcaa tcataaaagt ctaattaatt
     9181 ataataaagt gtaaataaaa aggtgtaaat aaatgcttgc aaatttatct tattatgata
     9241 ttataaaaac ataaattata ttaaggagtg atcctgatgc gattgagaag aaataagaat
     9301 tacactacca ttattttaga agattttact gatcaagaat tacttattgt cggtaaaaca
     9361 aaagcagatt ttatcgaaaa gggaaatgct tcaatagtaa aagcttcaaa gattgctgtc
     9421 gaaaaatatt ttgattcttt agaagaaagt gctaaggaaa tttttaatgg agagaaagct
     9481 ggtagctatg agttagacgg tattagattt agcttgatcc caacaactga aaatattatt
     9541 ccgaagaaat tatattatat cttttcatct gaaaaacata cttcaatgaa gccttgtatt
     9601 tttgattggc aatggttgat tgatgctgta ttggatgtaa aatgttcaaa atacaatatt
     9661 actttggggt gttaattatg agtatgctag ttagaaaatt accattatct gtaagagaat
     9721 ttctatttta cttggtctgc ttaatcgcat cagggtttaa gctgatcgaa catattttta
     9781 tctttgctta tgtattagca atgatcgcat ttttatctgg cggttggtta attatttatt
     9841 tgttaagcta agttttagat caaaaataat taataaatta aacaataaaa taaccgttaa
     9901 taaatgtaat gaaaggacaa cgcctaacaa tcagcttgta ataagattat aaaataacat
     9961 tatattaaaa taagtgcttt ttaaaggaca aattaagtga atca
//
LOCUS       DAZUMV010000098         9978 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000098 DAZUMV010000000
VERSION     DAZUMV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.099"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG8FK_002108"
     CDS             complement(53..787)
                     /locus_tag="ACG8FK_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5097698.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1113..1523
                     /locus_tag="ACG8FK_002109"
     CDS             1113..1523
                     /locus_tag="ACG8FK_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS5097699.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1516..2217
                     /locus_tag="ACG8FK_002110"
     CDS             1516..2217
                     /locus_tag="ACG8FK_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS5097700.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2217..3803
                     /locus_tag="ACG8FK_002111"
     CDS             2217..3803
                     /locus_tag="ACG8FK_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5097701.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3822..4043
                     /locus_tag="ACG8FK_002112"
     CDS             3822..4043
                     /locus_tag="ACG8FK_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS5097702.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4040..4801
                     /locus_tag="ACG8FK_002113"
     CDS             4040..4801
                     /locus_tag="ACG8FK_002113"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS5097703.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5181..5690
                     /locus_tag="ACG8FK_002114"
     CDS             5181..5690
                     /locus_tag="ACG8FK_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5097704.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5765..6304)
                     /locus_tag="ACG8FK_002115"
     CDS             complement(5765..6304)
                     /locus_tag="ACG8FK_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS5097705.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6444..7055)
                     /gene="rpsD"
                     /locus_tag="ACG8FK_002116"
     CDS             complement(6444..7055)
                     /gene="rpsD"
                     /locus_tag="ACG8FK_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS5097706.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7317..8168
                     /locus_tag="ACG8FK_002117"
     CDS             7317..8168
                     /locus_tag="ACG8FK_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS5097707.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8196..8831
                     /locus_tag="ACG8FK_002118"
     CDS             8196..8831
                     /locus_tag="ACG8FK_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS5097708.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8851..9624
                     /locus_tag="ACG8FK_002119"
     CDS             8851..9624
                     /locus_tag="ACG8FK_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5097709.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
BASE COUNT         3096 a         1781 c         2082 g         3019 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaagaaacat caacacgaaa
      841 agcatcggtg cagtgtttcg tgtcggaagg atttctgcag tcttaacaaa tgtgaataaa
      901 aaaatgaatt ccattactta taagaaacca gaccaacagt aaaactccaa ctctttttaa
      961 aagttctcca caaatcacca agcttaacta taaatatacc aaaaaaaaaa cgtttttttt
     1021 ctcaaaaatt gtatcttaga aacagcatgt tttgaaactt tcagaaaagg tttgttagta
     1081 aaatgaaagc tatacgtaag gagtgttgtt tgatgaaatt ttttcaagag tttttcatta
     1141 ttattttctt ttctgtatta ggagaaggca tacggatttt tgctcattta cctattcctg
     1201 gaagtatctt aggtattctt tttttatttt tagcatttga aatgcatctt gttgatcctg
     1261 aaaaaatcgg agcaactggt gattttttat tgaataatct agccatttta tttgttcctg
     1321 ctggtgtagg tttattggaa tattttgatg atattgcttc gatatggcct gttttactgg
     1381 gagcagtcgt cgtgtgttcc atcgtcacga tggttgcagc gggaaaaacg gcagaaggtg
     1441 tagaagcttt gctgggctat gtccgtaaga aaaaagcaga aagggtatct gtaaacgaaa
     1501 tggaggagaa tcaaattgac tgagtttttt gtgaatccat tatttggctt aagtttgact
     1561 ttaggagtgt acgtattatc tgacaaattt ttgcggaaat ataaactgcc tgttttgaat
     1621 cctctcgtat tatcgattgt tgttattatc ttatttttgc atttttctgg gatcagctat
     1681 aaagactata atatcggggg caattttttg actatgctga ttactcctgc gacagtggca
     1741 cttgccatcc ctctttataa aaacttccgt ttactgaaag agaattttta tcctgtaatg
     1801 gctgcgattt tagcaggaat agtcgcaaat tgcttggtta gtgtagggtt cggttacttc
     1861 ttctctcttc ataaagagat ggtgatctca ttactaccta aatctgtaac gacggctatt
     1921 tctgtcgatt tatctcatac gatgggcggg gtgaatgccg tgacacttgc tattgttgtc
     1981 tctacaggta tttttggttc gctgatcgcc actcatattt tccgcctatt caagatcgaa
     2041 agtccagttg ctcgcggtgt ggcgttagga agtacttctc atgctattgg tacagcaaaa
     2101 gcaatcgaga ttggtgaaat agaaggaatc atttccggac ttgcgatatg cgtcaacgga
     2161 atattgaccg ttttattgct accgttattc tttcagcctt ttgctggctt gttttgatgc
     2221 gaaaaccact attttatgct acaataacca aaacgttcga gcataaaaaa ggagggaaag
     2281 acgtgtattt gtcttccgag tatcttaaac aatttgataa agatctatat tataagattc
     2341 tcctcctaga aagttttgag gatcaagctt ggcataccgc tgcacaatta gcgcaagttg
     2401 ttcagctgga tgcccgcagc gtgtcgaaat acctgaacga gctgtctaaa aactatcagc
     2461 agtttagcgg taagacacac cctttgttta caaaaaatca ccgttctggt tataactttt
     2521 atgatacgct cgattcaatt gaacatgagc gttttttgat ctatctcgtg caaagtacgc
     2581 tgaaattcca gttgctgcac gacatttttt ttgaagaatt ccatacaatg taccaatttg
     2641 cgcaaaagca ttacattagt gaatctactg ctcatcgaaa aatcaatgaa tggaaacagc
     2701 aactgcaaac ttacggaatt cggctgcaac gaggtacata tatcgctcaa ggcgaagaag
     2761 aaatcattcg actttacttg catatgacat tctggcagtt gtttcgaggg aaaatctggc
     2821 catttgaaac gatttctcaa atggacgtaa agaatatggc agaacatatc atggcttttt
     2881 ttaatgttcg tctgaacgag atcaaaaaaa gacgattgga atatatgcta ggtgcctttt
     2941 ttttaagaaa atcacagaag cattatgttg tattgaatga gaaaaaaaga cggctgattt
     3001 ctgacaatct gttgttccag cgattttgcc aggtcatgga gccggtattt cctaattatt
     3061 ttcaagtaga agacgagtta ggcgcattat ttcttgtact gatgactcgt gaagaatatt
     3121 atagtgatcc taagatacgg aagaaaattt ttgattttca tcaacaagca aaaacaccgc
     3181 cctttacagc cctttcagaa gcaaaagccg ctctttctct ttatcaggaa gagcaggggc
     3241 tgccagcaga aaatctaacc ttcgaggcag aaaactattt attcagcagt cattttttcg
     3301 cctatctttt tcctaatgcg aaagaaacga ttgatggcaa tagtagtgat tttattaatc
     3361 atttagttat tgaaaacaag gaattgaaac aatggctcgt acattttttt gaaagtagac
     3421 acaaacatcc caatcatctg gcatttaaaa atcatgcgtt tttgatgggg cggtatctga
     3481 ctgtgtttaa aacattagga gcctttactc cacagcttcc aaagatcacg atccttttaa
     3541 tgacagattt cccattattt gaagagcaac tgttagagga gggattacgg acatttttcc
     3601 gtaatgagta tcagctgatt tttctgccaa cagattatcg ggggagagaa gttgatcttc
     3661 tgatttcgac aagtaaagtc caccgcaaac catgggcgga tcttgactat tttattgtga
     3721 ctgaagaact aaaattaatt gattacatcc aactatctca aaaatttgaa atgattcaaa
     3781 aacaaaaact attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt
     3841 taccagaatt gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt
     3901 atgttgttct cgtatccgat gatgaatttg atggctgtcc ggtgacgccg tacttattga
     3961 tcaaaacaag tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag
     4021 atgattgggc gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt
     4081 tatcacagga gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga
     4141 tgcttttgta tttggattag atcaaaagtt cggtaaaatg gaagaaattt ctttgcgatt
     4201 tcctgaaact tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc
     4261 ggtatcgaaa tgccaaaatc attttggtga agttacgatt ttactgaata cagcggggat
     4321 attggatgcc tacaaaccct tgatggaaac tgacgaatcg ctgtgggatc tgatttatga
     4381 gacgaatgtg aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgcca
     4441 aaaaagcgga acgatcatca atatggcttc gattgctggc ttgattgctg gtggaggtgg
     4501 gatcgcttat acaagcgcga aacatgcgat tgtcggattt accaaacaac tcgctctgga
     4561 ttacgcagga cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat
     4621 gaatgcggct gattttgaag ggaaaggtga gatggcagag tgggtggcaa atgaaacacc
     4681 agtcaagcga tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc
     4741 tcaagcaagc tacatacaag gaacgatcat cccaatcgac ggcggatggt tgttaaaata
     4801 accttgtgtt tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg
     4861 ataaaaaaag tcgatgactg ttcccgacat tcgatgccga acagccagtt caaaaaattc
     4921 tttttaataa tcgttaatat tcgaaactaa gcggcttttc acgacctctt gatataaacg
     4981 atattcaaaa gcttgcaact gcggtaacaa gtccagccaa atgaaaaata taaggagcta
     5041 tttttatttg accattctta ttacttgttg cagggcagct ttttcactac cttctgcgac
     5101 acttcagttg gaattttctt cccatccttt ggtcgcggtc attaccaaga taaaattatc
     5161 ttgcaaagga gaaatagaac atggaaaaaa ataatacccg ttcagccaaa cgctggacaa
     5221 cgacagatgt gactcggatg gcgctagtca ctggtttata tgtggcagtg acgctggttt
     5281 tgtctgtctt tagctttgga gtgattcaga ttcgtctctc ggagatgttc aactatctgt
     5341 ccttgtataa caaaagatac atcgtcgcgg taacagcggg agtagctttg gctaatattg
     5401 cttctcctct tggactgata gatgttgcag taggaagcgt cagcacgctg cttgtattgc
     5461 ttatcaacta taaaatcact agtcgcatca aaaacatgaa aatcaaaatg gccgtgacag
     5521 catgcgtatt tgctttttcc atgtttacag tagctggtca gctaacgatt ttatatcaac
     5581 tacctttttt cttgaattgg tgggtgattg ctttaggaga attggcatca atgattgtcg
     5641 gtgggataat tatttattgg atcggtaaaa aagtggattt gtcaaaataa agatctgttt
     5701 aatttaaaat agggcagcat ggaagagact gaatgttgag tcttcttcca tgctgctttt
     5761 ttgattaatt tgtatgaaga aatcgattta gccgtttttt catcaagata ccgatataag
     5821 ctgctccagt tgccttgatt gcttccggca gtaaaaacgg cagtagtcca ctggcaaatg
     5881 cagttgtaaa agtaagatca ccacttattt tcagccagat cgttccgcag atcaatgcag
     5941 caagagagcc cattagattg gcaaagattg catataagta atgagcatcc gttctttcga
     6001 tcatccaacc agttatccac gtttgtaaga gaaatccaat caaatatccg ccagttggcc
     6061 caaataaaac agctatacct ccagtcatcc ctgaaaatac aggaagacca atcaagccaa
     6121 gtaaaagata aactccaaca gatatcaatc ctagttttct tccgagaaaa gttgctgtta
     6181 atccaatagc aaatgtttgc cctgtcaaag gaaccaaacc aaaaggaatg gtcatctgag
     6241 aaaggacaga aataatcgcg gcaaattccg cagcaagtat caattctttc aatgttcggt
     6301 tcataacgcc ctcttttcgt taacttaata tttttatttg gttaacgaaa ttgtaaaaca
     6361 aagtgattac tttgtaaata gaaaatataa aaaaagacca gctatccaat agatagcggt
     6421 ctttttcaag aaagcttttg aaattataag ctttggttgt agtattcaac gacaagagct
     6481 tcgtcgattt ctggatatag ttcgtcacgt tctggtaaac gagtcaatga accttccaat
     6541 ttttcagcgt caaagcttac gaatgctggg cgtccaacag ttgcttctac agcttctttt
     6601 actgttacga tgttttgtga tttttcacga actgagatca cttgacccac ttctacgtgg
     6661 tatgaaggga tatcaacgcg tttgccatca actaaaatgt gaccatggtt aactaattga
     6721 cgtgcttgac gacgagtcgt tgcaagtcct aaacggtaaa caacattgtc taaacgttgt
     6781 tctagtaaga tcatgaagtt aacaccatgt ttaccttctt tgattttgct agcttttacg
     6841 aacaagttac ggaattgacg ttcgttcatt ccgtacatgt gacgaagttt ttgtttttca
     6901 gttaattgca tgccgtattc tgattgtttg ccacggctat taggtccatg ttgacctggt
     6961 gcgtaagggc gacgtgctaa ttcttttcct gtgcctgaca atgagatgcc taagcgacga
     7021 gaaattttcc atgatggtcc tgtataacga gacattaaaa tttcctccaa tgaatataag
     7081 tttttggagt aaaataatcc gttgaaaaat tcataatcat atagtttgtt cttcaatctt
     7141 cacctttgca gccgcggtta cacaatcgaa cccgagacgg ggcaacaaac tagggatgag
     7201 ttattatttt tctgctgcat tattttacac aaattacagt atatgataga tcatccacga
     7261 atgtcaagat aattagtaca taaaaacgct ttacaaacct aggctgtctt tataaaatga
     7321 gagggtatgt gcaaatggaa aaaagcgaag gaagtttaag gagaaaatca atgaatccgg
     7381 tatcaccatt atttgagaaa atcgatgctt caattgaaaa gaaggcgaat ctttttgaaa
     7441 acagcttttc acgttacgct gttcgagcgg ttctcgcctg tttgtttttg actttaggaa
     7501 cggctgtggc atttgcgatc gcgatgaagg gggaagatat cgcgcatggt ctaggaaaga
     7561 ttttatacgc atttatgttc agctggggtc tggtcatgat tctatacatg aacgccgaat
     7621 taggaacatc gaatatgctt tatatgaccg tgggtgtttt tcggcagaga cttgctttaa
     7681 gtaaggcagc caagattcta tttgcctgta tttttttcaa tatgattggc ggtatattat
     7741 gtggatttct aatttctatg actacgccat ttcaagattt aacgttggac aactttatgt
     7801 tagaatcaat agcagtaaaa ctagcaaaac cttcagcaac gattttggtg gaagggatgt
     7861 ttgcgaatat cgttgtcaat accgctgtac tgatcagcat gcggatgaaa gaggatgtcg
     7921 gaaaagtgat cacagtcgta ttcatcattt tcatttttgc ttttttagga tatgaacacg
     7981 tgatagcgaa ttttcctgcc tttacgctta cttactttgc ttctcacgga cagatggatg
     8041 gaatgacagt cagcaatgtg ttacataatt tgttttttgc tttggcagga aattatattg
     8101 gtggcggttt agtaatggga ctgggatatg cttggctgaa tcagtcaaaa tcatcctatg
     8161 tggactaaaa agtcgttcat taaaggggaa gtgccatgga agttttagtt tttccaaaaa
     8221 atgaggaaaa agcgttacaa atggaaaagt acatgaaaaa tcttttttcc tttgctggag
     8281 tacctaagcc ggaacgcgct cttttacaac aaccgttact ggatgagagc aaaaatttat
     8341 ctatttctga agttttagct tttgttcatt attattatga acaaatcgaa agagagtatc
     8401 agtatgtagc gatcgattta gtccagaaaa acatcaaacg gcttacattt gaagacttag
     8461 tcgtattgtt gccactagtt cagaaaaaag aatggtggga cagcatcgac tcttggagaa
     8521 aagtatacag cacatggtgt aaagcctacc cagagcagcg tgaagaagtc tttcaattgt
     8581 ttgaaaaaaa ggaagatttt tggctacgtc gaatagcaat cacgttgcaa ttaggttata
     8641 aacagcaaac agacacagga ctattgatcc gagcaataga cgaagatcgt caaacaaagg
     8701 aattctttat ccaaaaagca attggttggg cattacgcga ttatagtaag accgatgccg
     8761 attgggtaaa aagacagctg acaacagatt tgagtaagtt agctgttcga gaggcaagta
     8821 agtatttata agaaagcgag acgacaagag atgagacgca aattatttgc ttttgatatt
     8881 gatggaacgt tgttagattc aaacaaacaa gctttagata gcacaagaga agcattggaa
     8941 aaattacgca aagccggtca tctggtcacg attgctacag ggcggagccg atttcatgcg
     9001 caagagatca ttcgcgatct tgattttacg aactacattt tatgtaatgg agcggccggt
     9061 tttttagacc atgaacaagt atataagaat ttactagatc gcgaccagtt ggatgcgttc
     9121 gttcaagaag cgcatcatga gcagatcgat acagcttttg tcagcttaga caatatcaag
     9181 cgttcttctt ccaatgatgt aacggtgatg gaagaagcga tgcattcttt cggtgctgtc
     9241 ttgcctgaat tagatcagtt ttttgcagag gaacaagaaa tataccaagc gttagcattt
     9301 ttcgatcaaa catatgatgg gaagttctct tcatatgata aaattcgatt tgtccgctgg
     9361 catgagaaca gtgtagatgt cgtacctaaa aatggttcta aagcagcgac aattctgcac
     9421 ttagcagaac aagtaggcat ccctcatgaa gacatcatca gctttggcga tggacaaaac
     9481 gaccgtgaaa tgttgcgcat gtcaggaatt gggatagcta tggggaatgc tgtgccagaa
     9541 gtacaagcag aagctaagat ggtcactgat acaaacgatc aggatggaat ttggaaggca
     9601 ttgaaagagt taagcgtgat ttaaaagagg ttgtgaaaaa gctgttctgc atcaagtaat
     9661 aagcccagcc aaacaaaaat agctcctcct atttttcgtt tttctgggct tattaccgca
     9721 gatgcaagct ttcgaacacc gtttaaggaa gaggtcgtga cagaagtggt cagtttcaag
     9781 aaataagaag ggatttacga aaattgcttc tcaaattttt gtaaaattac ggcttatttc
     9841 tgaagaagta gatgaaacaa cgtttattcg ggaaatggga ctgtgacaag atgtttgtca
     9901 tagtcccatt tttttatttt atttttcaaa actaagaaat accaggaaag agttcctttt
     9961 gtttgacctt ttctttct
//
LOCUS       DAZUMV010000099         9963 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000099 DAZUMV010000000
VERSION     DAZUMV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.100"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACG8FK_002120"
     tRNA            8..81
                     /locus_tag="ACG8FK_002120"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="ACG8FK_002121"
     tRNA            101..173
                     /locus_tag="ACG8FK_002121"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="ACG8FK_002122"
     tRNA            201..274
                     /locus_tag="ACG8FK_002122"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="ACG8FK_002123"
     tRNA            288..361
                     /locus_tag="ACG8FK_002123"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="ACG8FK_002124"
     tRNA            385..474
                     /locus_tag="ACG8FK_002124"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="ACG8FK_002125"
     tRNA            487..560
                     /locus_tag="ACG8FK_002125"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="ACG8FK_002126"
     tRNA            579..654
                     /locus_tag="ACG8FK_002126"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="ACG8FK_002127"
     tRNA            656..726
                     /locus_tag="ACG8FK_002127"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            739..812
                     /locus_tag="ACG8FK_002128"
     tRNA            739..812
                     /locus_tag="ACG8FK_002128"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:773..775,aa:Ile,seq:gat)
     gene            837..924
                     /locus_tag="ACG8FK_002129"
     tRNA            837..924
                     /locus_tag="ACG8FK_002129"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:871..873,aa:Ser,seq:gct)
     gene            946..1017
                     /locus_tag="ACG8FK_002130"
     tRNA            946..1017
                     /locus_tag="ACG8FK_002130"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:979..981,aa:Glu,seq:ttc)
     gene            1040..1129
                     /locus_tag="ACG8FK_002131"
     tRNA            1040..1129
                     /locus_tag="ACG8FK_002131"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1076..1078,aa:Ser,seq:tga)
     gene            1142..1215
                     /locus_tag="ACG8FK_002132"
     tRNA            1142..1215
                     /locus_tag="ACG8FK_002132"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1176..1178,aa:Met,seq:cat)
     gene            1234..1306
                     /locus_tag="ACG8FK_002133"
     tRNA            1234..1306
                     /locus_tag="ACG8FK_002133"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1267..1269,aa:Asp,seq:gtc)
     gene            1310..1382
                     /locus_tag="ACG8FK_002134"
     tRNA            1310..1382
                     /locus_tag="ACG8FK_002134"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1343..1345,aa:Phe,seq:gaa)
     gene            1387..1457
                     /locus_tag="ACG8FK_002135"
     tRNA            1387..1457
                     /locus_tag="ACG8FK_002135"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1419..1421,aa:Gly,seq:tcc)
     gene            1470..1543
                     /locus_tag="ACG8FK_002136"
     tRNA            1470..1543
                     /locus_tag="ACG8FK_002136"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1504..1506,aa:Ile,seq:gat)
     gene            1555..1626
                     /locus_tag="ACG8FK_002137"
     tRNA            1555..1626
                     /locus_tag="ACG8FK_002137"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1588..1590,aa:Glu,seq:ttc)
     regulatory      1815..1894
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1892..3085
                     /gene="metK"
                     /locus_tag="ACG8FK_002138"
     CDS             1892..3085
                     /gene="metK"
                     /locus_tag="ACG8FK_002138"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS5097710.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3215..4687
                     /locus_tag="ACG8FK_002139"
     CDS             3215..4687
                     /locus_tag="ACG8FK_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5097711.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4770..5558
                     /locus_tag="ACG8FK_002140"
     CDS             4770..5558
                     /locus_tag="ACG8FK_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5097712.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5625..6293)
                     /locus_tag="ACG8FK_002141"
     CDS             complement(5625..6293)
                     /locus_tag="ACG8FK_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS5097713.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6408..8120
                     /locus_tag="ACG8FK_002142"
     CDS             6408..8120
                     /locus_tag="ACG8FK_002142"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097714.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8123..9895
                     /locus_tag="ACG8FK_002143"
     CDS             8123..9895
                     /locus_tag="ACG8FK_002143"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097715.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2901 a         1781 c         2277 g         3004 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccacc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gtttttatgg gcctatagct cagctggtta gagcgcacgc ctgataagcg
      781 tgaggtcgat ggttcgagtc catttaggcc cattagtaca aaatcttatt ggttttgggg
      841 aagtactcaa gtggctgaag aggcgcccct gctaagggtg taggtcggga aaccggcgcg
      901 agggttcaaa tccctccttc tccgtttcaa tattttgttt tacctggccc gttggtcaag
      961 cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta cgggtcatta
     1021 atttaatata gttttacatg gaggattacc caagtccggc tgaagggaac ggtcttgaaa
     1081 accgtcaggc gggtaaaacc gtgcaagggt tcgaatccct tatcctcctt tttcatatta
     1141 tcgcgggatg gagcagtctg gtagctcgtc gggctcataa cccgaaggtc gtaggttcaa
     1201 atcctgctcc cgcaattaaa aactaaaaac attggttccg tggtgtaggg gttaacatgc
     1261 ctgcctgtca cgcaggagat cgcgggttcg attcccgtcg gaaccgtttg gctcggtagc
     1321 tcagttggta gagcaatgga ttgaagctcc atgtgtcggc agttcgattc tgtcccgcgc
     1381 cattatgcgg gtgtagttta gtggtaaaac cacagccttc caagctgttg tcgcgagttc
     1441 gattctcgtc acccgctttt agtttttatg ggcctatagc tcagctggtt agagcgcacg
     1501 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccatttattt tattggcccg
     1561 ttggtcaagc ggttaagaca ccgccctttc acggcggtaa cacgggttcg aatcccgtac
     1621 gggtcatgga agatactttt tgagtatctt tttcttttta tctgaaagaa tgattttaaa
     1681 aagcttagca gagttatctt tatggatgat tgtgctaagc ttttttggac agttctaatg
     1741 taaaatattt ttatattttt taagcaaaaa aatgaaaaaa tacgagcaga aaactgtttt
     1801 atctgctata cttggttcgt tgcaagttcc cgaaaggatc cgcaggacgg agatgccttg
     1861 taaccgaatt taactagggg gaatcattat aatggtagaa agacacttat ttacatcaga
     1921 atctgtttct gaaggacatc cagataaaat tgctgaccaa atcagtgatg caatcttgga
     1981 tgcaatttta aaacaagatc caacagcacg agtagcgtgt gaaacgtctg taacaactgg
     2041 tcttgtttta gtatttggag aaatatctac gacggcatat gtagatattc aaaaagtcgt
     2101 acgggaaaca ataaaagaaa ttggttatac acgtgcaaaa ttcggatttg atggagatac
     2161 agcagctgta ttggttgcga ttgatgaaca atctcctgat attgctcaag gagttgatga
     2221 agcacttgag atccgggatg aagataaaaa agacgtatta gatgagattg gtgctggtga
     2281 ccaaggttta atgtttgggt ttgctgtaga tgaaacacca gaattgatgc ctttaccaat
     2341 cgctttgagt catcgtctgg tacgacgctt ggcagatttg cgtaaatcaa atgaattaac
     2401 gtatttacgt ccagatgcaa aatctcaagt aacggttgaa tatgatgatc aaggacaacc
     2461 ggaacgcgta gatacaatcg ttatttcaac acagcatgat gatgcagtag ataatgaaac
     2521 aattcgtcat gatgtcatcg aaaaagtagt gaaggaagtt attccagctg aattattaga
     2581 tgatcaaacg aaatattata tcaatccgac tggccgattt gtcattggtg gtcctcaagg
     2641 ggatgccgga ttaacaggaa gaaaaatcat tgttgatacg tatggcggtt atgctcgtca
     2701 tggtggcggt gctttttctg gtaaagatgc gacaaaagtt gaccgttctg ccagctatgc
     2761 tgcacgttat attgctaaaa acattgttgc agcagggctt gctcgcaaag cagaagtaca
     2821 gctagcttat gcgattggtg ttgctcaacc tgtttcgatc tcaatcaata ctttcggtac
     2881 tggaacggtt ccggaagaag aactgattgc tgcagtgaga gaaaacttcg atcttcggcc
     2941 agcaggaatc atcgagatgc ttgatttgcg tcgtccgatt tacaaacaaa cagctgctta
     3001 cggtcatttt ggtcgtacag atgtagattt gccttgggaa catacggaca aagtagatgc
     3061 gttgaaagca agtttagcaa aataagttat attcagctgg aacgatcgcc aagtggtggt
     3121 tgttccagct ttttttatgg cagttaacct cttgaaaaag atagtaatta tttatctatt
     3181 tttagatcct gtcataatga aagggaggaa taagatgaaa aaagaaacaa atgttacttt
     3241 ggtcacagtg agtattttca ttgcaacgtt catgacggct attgaaggta caatcgtaac
     3301 aaccgctatg ccaacgattg tcggttcatt acatggaatg gaaatcatga actgggtatt
     3361 ttcgatttat ttgctgacaa acgctatgct tactccgatt tacggaaaac ttgcggataa
     3421 agtgggacgg aaaccaatct ttatttttgg gacattattg tttattatcg gttcgttatt
     3481 ttgcggttta tctcaaagca tgattgggtt gattgctgct cgtgcgattc aagggatggg
     3541 tgctggggca atgatgccag tagctttgac gatcatcggc gatctttatt ctattgaaaa
     3601 gcgagcaaaa gttttaggtt tgaatagttc agcctggggg attgccagtg tttttggacc
     3661 gcttgcggga ggattcatcg ttgaaacgat cagctggcac tggatctttt ttatcaatgt
     3721 gccgatcgga attctattaa ttattttgat ttggctatat ttaaatgaac caaaaatcat
     3781 acatgagtct aaaccaatgg atatttttgg tagttttagt ttgatggcca tgcttgtctc
     3841 attgctgtta gggttccaat tattaggaga cgaaggaatg agtccgcttg tttctgcttt
     3901 gctgctaggt agtatcgtaa tgttaggtat ttttgttttt gccgaaaaaa gagctaaaga
     3961 tcctgtcatc atgatgcatt tattcaaaaa tccactattt gttgtggtca acttggctgc
     4021 agcattggct agtggttttt tgatgggaat cgatgtttat atccctatgt ggatgcaagg
     4081 tgtattaggg ctgaatgcag caattggtgg gctggtatta gcaccattat cactagtctg
     4141 gatggtcggt tctttccttg caagcaagtg gatgactcaa atgaaaactc attgggtctt
     4201 gagaattgga ctactaatcg cagctattgg gggcgcttgg ttgtatctta tgcctttcag
     4261 aagtaactat ttatggtttt ttgctatatc aacggtcatc ggtataggct taggtgtgac
     4321 catgactacc tcgactgtca gtgcacaaaa tagtgtatct cgtgaccagc ttggtgtagc
     4381 aacttctttt aatacgttag ttcgaacaat tggtcaaaca gtcatggttt ctatttttgg
     4441 tttattgata aatggggtaa ctaaacgaga acttgcagca gcaaatttaa caaatgatgc
     4501 agatatcatg aataaactgg tgaatccaca aacggctaaa tatattgatg cggatatttt
     4561 gttaccatta agaaaaattc tttatgatgg gttacattcc gtttatctag ttggcttgtt
     4621 actcgtgatc attgctttgg tcttaacgct tttcgttcat gaaaaaagtg gtaaaataaa
     4681 acagtagttt cggttaagat atgtacgtag ccgaatcatt caaatttaaa gttattcaaa
     4741 aataacttct tactaaggaa ggcgaaaata tggcatttat tcaagcaaat atttattcga
     4801 atgtgttaga aatggaagtc aatctaaatg tgatactacc gcaaacaaca aaaaaggtga
     4861 ttggcaccag ttcagagcaa gagacaacgg atatacctgt tttatattta cttcatggaa
     4921 tgggtggcaa tcatagtgta tgggaaagac gtacgtcgat tgaacgttat gtagcaaact
     4981 acggattagc tgtcatcatg ccatctactg atcttggctg gtacacagat acagtttatg
     5041 acatgaagta ttggacattc attgcagaag agctacctga gatttgtcat gaattattcc
     5101 cgcaattaac aagaaaaaga gaaaaaacat atgccgctgg attatcgatg ggcggatatg
     5161 gcgcgttaaa actaggcctt gcaaaatcag aaaatttcgc agcagtaatc tctctttcag
     5221 gcgctgtttc tgtaggaaat cgaatggatg atcttttgat ggttcgtaaa gcatcttttt
     5281 gggaagggat ctttggacca ttggatcaaa tcgaaggttc aaaaaatgat ccgcaatact
     5341 tattgaaaga actagtaaaa agcggaaaac cagttccaag attttatatg gcttgtgggg
     5401 aatctgattt tctttatcag gcaaatcagg aaatggtatc tgctatgcag gaacaaggct
     5461 tggcggtcac ctttgaacaa ggacctggcg atcataactg gatcttctgg gaccaatgga
     5521 ttcaacgtgc attggcgtgg ctaatggaag aaaaataaaa aaagagtctt aaaaagatag
     5581 ctcctataga gatcacaaac atagtgatct ctataggagc ttttttactg ctcgttttct
     5641 cttcttttat tttgttggca taagccttca atataggttt gtgcactcgt tagaaagagt
     5701 gctttttcat tcaattctgc atgggactgc atactttctt taaatccctc taattgattt
     5761 tcaactacat aattacgcat aaatagaact gtttctttca aaaattcatt tccgtccata
     5821 attttttgtc gaagctgctg ttcatgctgc tgatccatta actgtccaat caataaattg
     5881 tggagagagg caatggcctg atgactttgg gtgactgaaa atccttcacg aacaagtaat
     5941 tggatcattt gctccaaatg ttctaatcga ctcggaaaag cgggcacagt attgatttct
     6001 aattctgccc cacaagggaa acgtgtataa aggtcatagt aattttccat atactgcagc
     6061 aactgttcat tccaaggcaa agttggatct ggaagcgcta attcttcttc cattgtttca
     6121 gccatttttt gcaagagctg ttgtttgttt ttgaaatacc aatataaagc aggtgcttga
     6181 acatgaattc tgtttgcgat gtttcgcatg gaaagctgcg aaagagcttg tgtttcagcc
     6241 aggatatcaa aggcggcctg aatgatgatt gcttgagaaa gctttatttc cattttatca
     6301 acctcgtgta ttgcattatc tttttaaaca tggtaaattg ttagtcggtc taattttaca
     6361 gtgttaaatt agtcctgtca atttaaacaa aggggtgtaa tcaacgaatg aaattaatct
     6421 tacgacatgc gaaaaaatat aaagtagctg tttttatttc cttgctgtcc gtagcagtca
     6481 tggttgcagc agcattatgg caacctaagt tactacagca agtattggaa gctattatta
     6541 cagaagataa tgatgaaatg aaaacaattg gtatttacct gatcagtctg gctttgctag
     6601 gattagccgc aggtgtaacg aacacgattt tctctgcaaa agtagctcaa ggcgtaagtg
     6661 cagatatccg agaagaagca tttcgaaaga tccagacatt ttcatttgga aatatcgaac
     6721 agttctctgc tggaaatctt gttgttcgtc taacaaatga tatcacgcaa atccaaaatt
     6781 tagtgatgat ttctttgcaa tcactttttc gaattccatt cttatttatt ggtgccttca
     6841 ttttagctat gatcacgatg ccgcaattat ggtggatcat cgtagcgctt atcgttacgg
     6901 tatttctgat tacggcactt tcatttacaa ggatgggcaa gcacttcatg atcattcaaa
     6961 aactgattga tcgtgtgaat agcattgcaa aagaaaactt actagggatt cgtgtagtga
     7021 aatcttttgt acaagaagaa aaccaattga agtcattcag caaagtaagt gaggacttaa
     7081 caaaacacaa catcatagtc ggtacattat tttctgtgat gatcccgtcc ttcatgctgg
     7141 ctgccaattt ggctgtggtt ggggcgatct tttttgtgag cgatcttgtt aaagatgatc
     7201 ctacgttaat tggtggaatt gcttcattca tgaactactt gatgcagatt atgatggcaa
     7261 tcattattgg cggaatgatg atgatgatga cttcaagagc agccgtatcg ctgaagcgga
     7321 ttgcggaaat tttagatgct gaaccagatc ttacttattt agacgttcca gaacaagagt
     7381 tgacgggttc tgtcactttt gaccatgttt cttttcgtta tcctggtgag gatacagata
     7441 cgcttaaaga tatcagcttt tctatcaaac ctggagaaat ggttggtatc gtcggggcga
     7501 ctggtgccgg gaagtcaaca ctagcacaat tgatccctcg tctatttgat ccgacagaag
     7561 gcaaagttga agtaggtggt gtagatcttc gtcaagtaaa tgaaaagagt ttaagacaaa
     7621 cggtttcctt tgttttgcaa aaagcaattt tattctcagg aacgattgct caaaacttgc
     7681 gccaagggaa aaagaatgca acagaaaaag agatggaaca tgcttcttct attgctcaag
     7741 caaaagaatt catcgaaaag ttatctgatc gttacgaagc accaatagaa gaacgtagca
     7801 gtaacttttc tggtggtcaa aagcaacgtt tatccattac tcgtggcgtt atcggtaatc
     7861 caaagatctt gatcctagat gacagtacga gtgcactgga tgctcgctct gaaaagctgg
     7921 tccgagaagc actcgatcga gatttgaagg gaacaacgac gattgtcatc gcacaaaaaa
     7981 ttgcttctgt tgtcaaagca gaccgcatct tagtattgga tgaaggcaga ttagtcggcg
     8041 aaggaaccca tgaggaattg gtagctggaa accgtgtcta tcaggaaatc tttgaaactc
     8101 aaaaaggaac ggaggagtaa gtatgactga tctaataaaa gcaagtaaat ttttctatca
     8161 ctatttgaag cggtacaaac tctccttttt ctttatcttt atcacagtga tcattgctac
     8221 ctatctacaa gtaaaggcac ctcaatacat cggggaagct tttcaagaat tagctaatta
     8281 cgtaggaggc ttgatgcaag gcgtagacga taaaagcaaa tttgtttctg taatatggaa
     8341 actcttgctg ttttatgtac tagctagtgc agccaacttt atctatagta ttttgttcac
     8401 acaagtagta gggaagtcaa cgaaccgaat gcgtatcggt ctattcaaca aattggaaaa
     8461 attgacgatc cgtttctttg attctcatca agacggtgaa attttaagtc gttttaccag
     8521 tgacttagat aatattcaaa atagtttgaa ccaagctttg cttcaagtca tcacgaatgc
     8581 tgttttactt gtcgggatct tgatcatgat gttccgccaa aatgtagagt tagcttgggc
     8641 tacaattgct tccactccag tagccgtttt gatagctgtc ttgattatcc gcaaagcacg
     8701 gaaatatgtg gatcttcagc aagatgaagt ggggaaacta aatggttata tggatgaaaa
     8761 aatcagcgga caacgtgtca tcatcacaaa tggcttacaa gaagaaacga ttgaaggatt
     8821 cttagagcat aatcaaagtg ttcgcgaagc aaccttcaaa ggacaagctt attctggtct
     8881 actatttccg atgatgcaag gaatgtcact ggtcaataca gcgatagtta tctttttcgg
     8941 cggctggtta gcattaaatg gtgatttgga acgttcggtg gcattaggct tagttgtgac
     9001 attcgtccag tattcacaac aatattacca accactgatg cagatttcat ctggatacag
     9061 catgatccaa ttagcgatta caggggcaag acgattgaat gaaatatttg atgaaaaaga
     9121 tgaagtcaaa ccagaaaacg gtaaagcatt tgatggtgtt gaaaaaggtc ttgcgttgca
     9181 gcacgtagat tttggttatt ctccagaaaa actagtcttg aaagatgtct cgatcaatgt
     9241 ggacaaagga gaaatggttg ctcttgttgg tccaacagga tcggggaaaa cgacgatcat
     9301 gaatctgcta aaccgttttt acgatgtcaa tgatggttca gtagtcatcg atggaataga
     9361 tatccgagat tatgatcttg atgcattacg tagacatgta ggaatcgttt tgcaagaatc
     9421 tgtattgttc tccggaacga tcaaagacaa tatcacattc ggcaaaccgg acgcttcaga
     9481 tgaagaagtc atcaatgcag caaaacaagc gaatattcat gacttcatca tgacgttaga
     9541 aaaaggttat gatacagaga tatccgaaga aaataactta ttcagtacag gtcaaaagca
     9601 gttgatcagt attgctcgaa cgatcatcac aaatccatct ttattgattt tggatgaagc
     9661 aacaagtaat gtagacacgg taacagaagc aaggatccaa aaagcaatgg atgaagcaat
     9721 caaaggaaga accagctttg tgattgctca ccggctgaaa acaatcttga atgcagatag
     9781 aatcgttgtc ttgcgtgatg gggaagtcat tgaagaaggc agtcatcatg agttattagc
     9841 agaaaatgga ttttatgcgg aactttatca taatcaattt gtatttgaaa gttaaattaa
     9901 aagaacctcg ttataacggg gttcttttta tatattttat gttgtttgtt tttataaggg
     9961 gca
//
LOCUS       DAZUMV010000100         9937 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000100 DAZUMV010000000
VERSION     DAZUMV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.101"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..614)
                     /gene="rlmH"
                     /locus_tag="ACG8FK_002144"
     CDS             complement(135..614)
                     /gene="rlmH"
                     /locus_tag="ACG8FK_002144"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS5097716.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1042..2331
                     /locus_tag="ACG8FK_002145"
     CDS             1042..2331
                     /locus_tag="ACG8FK_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS5097717.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2370..2975)
                     /gene="ytpR"
                     /locus_tag="ACG8FK_002146"
     CDS             complement(2370..2975)
                     /gene="ytpR"
                     /locus_tag="ACG8FK_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS5097718.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(3171..3635)
                     /locus_tag="ACG8FK_002147"
     CDS             complement(3171..3635)
                     /locus_tag="ACG8FK_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5097719.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3657..3977)
                     /locus_tag="ACG8FK_002148"
     CDS             complement(3657..3977)
                     /locus_tag="ACG8FK_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5097720.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(4120..5199)
                     /gene="pepA"
                     /locus_tag="ACG8FK_002149"
     CDS             complement(4120..5199)
                     /gene="pepA"
                     /locus_tag="ACG8FK_002149"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS5097721.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5399..5749
                     /locus_tag="ACG8FK_002150"
     CDS             5399..5749
                     /locus_tag="ACG8FK_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5097722.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            5934..6887
                     /locus_tag="ACG8FK_002151"
     CDS             5934..6887
                     /locus_tag="ACG8FK_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5097723.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            6900..8687
                     /locus_tag="ACG8FK_002152"
     CDS             6900..8687
                     /locus_tag="ACG8FK_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFS5097724.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            8674..9876
                     /locus_tag="ACG8FK_002153"
     CDS             8674..9876
                     /locus_tag="ACG8FK_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097725.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         2907 a         1867 c         1830 g         3333 t
ORIGIN      
        1 cgtcgtatca aagctcattc ataagtagta aattagtagt aaattgagtg gttttgacct
       61 tgataaagtg tgataagtcc agtttttatg cggataacta gatttttatg ctatttttct
      121 cttaaaaaat atcattattt gtggtatgga gcccccgcgt tgatccggaa ggcacggtag
      181 atttgctcga cgaggatcag gcgcatcaat tgatgtggat acgtcatttt gccaaaagag
      241 atctgagcat tgcttctttt catgacggct tcgctgagtc ctaaagaacc gccgataaca
      301 aaaacaaggt ggctttttcc ttggatacct aattggtcga tttgtttagc aaaagattcg
      361 cttgtgggat ttttcccttc aattgctaag ccatagacgt attcttgttc tttgattttc
      421 gctaagatcc gttctccttc tttttctttt acttgtctca tttgcgcttc gcttaaattc
      481 tccggggctt tttcgtcagg aacttcgatc aattcgattt tcgcataggc attcagccgt
      541 ttgacatact cattgatacc ttggatcaag tatttttctt ttaattttcc tactgaaata
      601 attttgatat tcatgttttc tctcctttga tcttcacgct cactttgttt attataatca
      661 attttttcgt ctatatgttt attttgacta tttttgttaa aaagatttcc ccaacagtta
      721 tgcacaacat ccacaggttt atccacagaa aacagcgttg ttttacctta tttcatcaca
      781 ttcattaggc aaacatacgt tttataagaa tttatccact ttatacacag gctgtggata
      841 tgttttatcc cttatttttc caatcctatc cgcgtagttt tacacaatat ctgtggaaaa
      901 agaaaatcaa atattttcat ctgaaaacaa tgatttttcc taaagttttt cataaaaagt
      961 tcaaagtctc aaagtagact aaagtcaaca aataagacta tactaacagt agataaatga
     1021 atgtaattgg aggaggaaca catggataga aaaaatgtga caccaaagat gaaaaaaaat
     1081 aaaaataata gcctctggcg taagttgggt ctggggcttg ttggagggat cgttggagga
     1141 ctactgacag ctggcatttt ttatgcagcg atgggcactg gcaacaatag ttctactgct
     1201 acaagcggca atcaaaattc tgccggtgaa acagttgtgg aaaatgtcaa agtcaatgta
     1261 gattctgata tcaccaaagc ggtagataaa gttcaagacg ctgtcgtttc tgttatcaac
     1321 ttacaaagtc aaaaccaatc aagcggtttt ggcggattat tcgggcagca agaagaaagt
     1381 agcagctcag atgatagtaa tttggaagct tatagtgaag gaagcggcgt aatctacaaa
     1441 aaagacggga atactgctta tgtagtcaca aacaatcacg tagtagatgg acagcaaggt
     1501 ttggaagtca tgatgaaaga cggtacgaag gtaaaagctg aattagttgg aacagatgcc
     1561 tattcagatt tagcggtgtt gaagatcaat tcagacaaag tcgaaacggt tgcctctttt
     1621 ggggactcaa gtgctttgaa ggttggtgaa cctgcgattg cgattggttc cccattagga
     1681 tctgaatatg cgaattctgt cacttcagga atcatttctt ccttgaaccg acaagtaaca
     1741 agtacgaatg aatcgaacga aacagtcaat atcaatgcga tccaaacaga tgcagcgatc
     1801 aacccgggaa attctggtgg tcctttagtg aatatcgaag gacaagttat cggtatcaac
     1861 tcaagtaaga tcgcaagtac ttctgaatcg acttctaatg taagcgtaga aggaatggga
     1921 tttgccatcc caagtaacga tgtagtgaat atcatcaacc aactggaaaa agacggaaaa
     1981 gtgacacgtc cagctctagg tatcacaatg gtcgacttat ctgccgtttc tactcagcaa
     2041 caagaacaaa tcttgaagat tccttcatct gtaactaacg gtgtcatcgt gacaaatgtc
     2101 caaacagcta caccggcaga aaaagctggg ttgaaacaat acgacgtcat tactaagatc
     2161 gatgacaaag aagtttcttc tggtgtagaa cttcaatctg ctttgtataa gaaaaaagtg
     2221 ggagattctg tcaaaatcac ttactatgaa ggctcgaaag aaaaaacaac tactgttgaa
     2281 ttgacgatcg atcaatcggc attaaaacaa aatcaaaata gtgaaaatta ataaaaaaga
     2341 ggttgtgaca aagacttgtc acaacctctt tattttggaa atacttctcc gactacggca
     2401 tcctcaggca atactaagat tccttttttt tctggagcat ttggcaaatg caattcacga
     2461 gcagaacaaa tcattccata gctggctacc cctcgcaaag ttcctggcca gatcatcatt
     2521 ccatctggca tcatggctcc tggttttgca acgacaactt tttgtccagc ttcgatattt
     2581 ggtgcaccgc aaacaatttg caatactgtc ccattgtcca cttctgtctc agtaatcgat
     2641 aaatggtcag agtcaggatg cgggacgcat gtttttacat acccgacaac gatcttcggt
     2701 tcatgatcag cttcaagctg ttcttcgaat cctgcttctt taatcaatcg attcaatttc
     2761 gctaattctt catctgttaa gcttacttgg ccgttccctt caattgttag atgatcggag
     2821 atatggaaaa agttccaagc aacaacttga ccttgttcat ctttgatccg agcaacattc
     2881 catttacgtt cgacagtgtt ttctgttcct ttatcgtcgg ctatgataac catcaaagtg
     2941 tctccgacgt gtgctttgtt ataagcaaaa atcatgttct ttcctccatt atcctttgct
     3001 gagatttatc ttaacaaaaa attgcattca ctgcatgaga tttttttcat gctttatttc
     3061 cgaagaagct tcttctggaa caccatttat tcgtaacaag ggactgtgac aagacttttg
     3121 tcacagtccc tttactgttt gattaaaaag gttatacaag ttccacttgc ttaattactc
     3181 ggctgttctt cttcgtcaga aacgatcaag acatcacaag gtgaatgacg tatgacgtag
     3241 ctggctacac ttcctgtcat aaagcgttcg actgcattta gtccagactg cccaaccatg
     3301 attagatcga ttgcatattt ttttggcaat ttgactgcca tggattcacg aacagagccg
     3361 tatgcaataa ttccttctat atttgtgtaa tcaacagatt ttccatattc tttacattct
     3421 tcgatcattt gcttggctgt ttcagtttgc tgatcgacta gattttgatc aataggagca
     3481 tagcccataa aattataaaa ttgctgatcg attacgtttg ctacatatac tttcccatta
     3541 ttacggcgag ccacttctaa cgcttttttg aaagcagcaa gtgcttgttc actaccgtca
     3601 atacctacta aaatgttctt atattcttga ttcatcttga cccctcctaa cagatgtcag
     3661 ccttttatta cactttctag gaacgattcg atttcctgtt ttgttttacg atctttattc
     3721 actagacgac ctaattcttt tccgtcttgg atcacgacaa aacttgggat accaaaaatg
     3781 ttccattcag cggctacatc tatgtacttg tcacggtcta cttcaataaa ttgccaatca
     3841 gaaaagtcat tttcgatttc aggcatttgc ggtttgataa aacggcagtc tccgcaccag
     3901 ccggcagtaa agaaaaatac actttttcca gtttctacgt agcctgctaa ttcttctaaa
     3961 ctatttggga taatcattct ttttcctcta ctttctgttt atgtattttt caattaaatt
     4021 atacaccttt ctttcgaact aacaaacatt gatcctcttt ttataaaaaa aatctgacaa
     4081 aaggttgctt ttgccagatt ttatagtttc actatcgcgt tatttcccgt atacgatcgt
     4141 gttcactgtt gatttatcta aacctttcaa tgtttgaatc aacatttctc gtgcagcttc
     4201 aaaatcgcga atactgaaca tcgtttgatg cgtatggata taacgtccca ctacgccaat
     4261 cactgtactt gggattcctt cattttgtgt atgagcagca cctgcatctg taccgccttt
     4321 tgaaacaaag tattgataag ggatgttgtt tgtttcagcg atatctagca agtactcacg
     4381 caaacgagga agcatgatca aacctggatc ttggatacgc atcaatgtac cttctcctaa
     4441 atgtccgaat gtgccatttt tcgtcactgt gtcatctgct gcagagcagt ctactgcaaa
     4501 gaacagatct ggcttgaatt tattgacaga agctttagaa ccacgcaagc ctacttcttc
     4561 ttggacattc gcgcctgcaa tcaatgtatg acctaatgtt tcattttgta acgcttctaa
     4621 agcttctaat accattgtac agccatagcg gttgtcccac gatttgctga tgatattctt
     4681 cccgttggct gttttgatcg tttcagcgta aggaacgatt gtgtctcctg gaagaacacc
     4741 gtaactcatt gcctcttctc ttgattcaaa cccagcatcg aacaaaatat ctgttacttc
     4801 tactgatttt tgtccgcttg ttcctcgtaa taaatgtggg ggaacggaag aagaaataca
     4861 tggataattc ttaccagtag aggttttaag tgtaaaacgt tgagcagaaa caacgtaagg
     4921 gttccatccg cctagaggta cgactttaaa aagcccgttt tcttggattt gtgtcagcat
     4981 gaatccgact tcatccatat gtgctgcgac cattacacga ggtgcatccg cttcttcatg
     5041 gtggcgcagc ccaaaaatgc cgcccaaacc atctaactgt aactcatcta ccagcggttc
     5101 catatgactt tccatatagg cgcgaacgtc ttgttcaaaa ccgcttgttc cttgtaattc
     5161 tgttaactct ttgattcgtt gaaatgtttt ttcttccata tgctattcgt ctaaatttca
     5221 gactaaactc ctctcagact tatattgatc tgcggataaa atatgatttt ttctgcagac
     5281 cgatcgtatt tattatagct tattttttct ggtagcggtt atttatttca aagaaaatat
     5341 gagagtgtgg taaaataaac acaaagagta gcaagtgaga agaaggaggt atatccacat
     5401 gtatgaagaa agcgaattaa gctattttaa aggcgggtta gccgttggga tcggtctagg
     5461 attattgagt ggggtagctt caacgatttg gtatcatcgc aaaaaagtgt tatcagcaga
     5521 ccaagtgctt gaaaatatca aaacatcttt tttaaaagaa ggcgcaatcg aaggtgcttg
     5581 gatctctttt gaaaaagaac ctattcggaa attcgccgtt cggtcgagtg cttatcgggg
     5641 tgggctttcc cggatagaag atggcgaaac tgtttattat gaatttttag cagatgctca
     5701 tacaggaacc gtattagaca tttcaagaaa aaagggcgca atagattaat acgtcaaaaa
     5761 aatgaaataa cgaatacgca cccactatct aaaagagagg tatcctatcc ttcttttata
     5821 gtgggtgatt tttataaaaa tagggctatt tcctcttgga atagctagta tttgtagtta
     5881 ttatacggta ttcttgaaat aagattagtt ttattatata aggagagtga caaatggaat
     5941 cacgtgaaaa aattgccgtg ctcattcctt gttataacga agaggcgaca atttcaactg
     6001 ttattgaaga ttttaaaagg gaattaccag aagcagatat ttatgtctat gataataatt
     6061 cgaccgatcg aacatacgaa ttggccgttc aaggcggagc aatcgtaaag aaagaaccac
     6121 gtcaaggaaa agggaatgta atccgtcaaa tgttttttga tattgatgcc gattattatt
     6181 tgatggtaga tggtgatgat acttatcctg cagaagctgc acatggtttg cttgagaaat
     6241 tacgttctgg cgaagcagat atggtcattg gcgatcgtct atcaaatgga acctattttg
     6301 atgaaaataa gcgtcctttc catgattttg ggaataatct ggttaaaaat acaattactt
     6361 acttatataa aacaaaaatt cgcgatgtca tgaccggcta tcgaggattc aatcgaattt
     6421 ttgtcaaaag cttccctatt atgagttctg gttttcaaat cgaaacagaa ttgacgattc
     6481 atgctttaga taaaaaattc aagttcgttg agattccaat cgattatcgc gatcgtcctg
     6541 aaggtagcga atcaaaattg aatacattct ctgacggatt caaagtgatc atgatgatca
     6601 tcaaaatgtg caaagattat aaacctttgc ttttctttgg gatctggacc atcttcttct
     6661 ttttatttgg attggtcatg gggattcctg tgattcgtga atatatgctg acaagcttta
     6721 ttacacgcat cccttctgcg attttaagta ctggattgat gactttagcc cttctttcat
     6781 tagtaacagg gttgattttg gatacggtcg tcacaaatgc caagaaagaa tatgaattga
     6841 atttatacca tgtctaccac gaatatcaaa ataaagaaat gaagtaggag gcctttttta
     6901 tgcataagaa aaaggagaat ctatttccta ctttttttgc aactttattt gcgtcaggtt
     6961 ttgctttttt tacttcttat ggtctaggaa ctttgaatcc aacagtaatc attggtttag
     7021 ccatggtttt gctacttgct tttaagcaat atggaataga gttgttttca ataaaaaaca
     7081 aagttgcagc tattgcccga ttgttttttt ctgcagcttt tgctcttaca ctttctattg
     7141 gcagacagat acaatttcag tatgtctcag caaactatac aaagacaacg attgctgatg
     7201 gaagtatttt ttactttctt gtttatttct ccatttttgt gtattctttt ttatttttac
     7261 aatcgattta tttatcaatg ccaaaactgg aaacattttt tactgctcaa aacacacagg
     7321 ctttctcttc aagaaaatat tttttattct ttactgtctt tttagttctg tgttggcttc
     7381 cttatctgtt ggcattgtat cctggagtgg ttttgcctga ttcgttaagt tcggtcgccc
     7441 aatctatggg tgatgttcct ataagcaatc atcatccagt cttattcacc ttactggtca
     7501 agctatttgt tcatgattta ccaactgata cgatcaatca cggtgtattt ttattttcga
     7561 tttttcaaac attgataaca gcttcagcga taagttattc tctttgctgg ctgttaaaaa
     7621 gaaacatcca tttttttccg gtactcatta gtttatgcta ttttgcattt gcaccggttt
     7681 ttccaatcta cgccttgaac atgcaaaaag atactttgtt ctctatcgct tgctttttat
     7741 tttccttagc ggtttttgac tttatttttc aacgagaaaa gccaacgatt ctcaatcaga
     7801 tttacgtaat catccttgct ttactgacca cctatctaag aaataatggg atttatgtag
     7861 taaccggcac aggtttgatt tgcggcttga ttattttgaa acaaaaaagg tatcaagcat
     7921 tctttcaagt ggtgctgggt tatacagtga ttgctggact actcatccac ccgttgatgg
     7981 ctcgttatgc tattcctaca gaaactgctg aacaattagg tgttcctttg cagcaaattt
     8041 ctagaactat tgttatggat ggtgacttgt ctaagaaaga taaagcattc atgaatcagc
     8101 tactcccgat aaaagattat gaagcgtatt ctcctagtct tgcagactca atcaagtggc
     8161 ataaaaattt taacaatgag tttttgaata cacataaaaa agaatttcta tcactatggg
     8221 caagaaattt accaagtaat ataaaaatct atacagatgc ttatatttta gaaacgttcg
     8281 ggttctgggt acctggtaca aaaaatagtt acggcttttt agatactcgt gtaaacacga
     8341 ataattatgg tatgaagcaa gtagatttga ttgaacatct tactggattg tcaggaatga
     8401 aaaaaatcat cgatgctcgt gatttctttg gttccggcac attactgtgg ttagctatcg
     8461 gcagtgcact tttagcaatc tttaagaaaa aatatcggta tttactcctt tttcttcccg
     8521 gtatgcttgt ttttgctact attatgattg ctactcctgt ggcttttagt ttacggtatg
     8581 tgtttgtttt agcattaggt ctacctgttt atatcctctc tcctttttta ctagataaaa
     8641 gagaaaaaat gaaaacagat ataggagata ctcatgaaac tatttaaaaa gatatttttt
     8701 gcttttgttc tcttgatcgc tagctattgc gtactactga caattacttt tttcattccg
     8761 cagcactcta ttgaatcgaa tgcagaaaaa tctctacaga tggttgaaaa agaaggaatg
     8821 tatccttcta tcaatcaagg aaatatgcta ggcactcggt tagataattt tactgatcat
     8881 ttgatgatac gaaaaacaaa agcagatcct gaattgaact ccttggagaa cgcgatgtct
     8941 atggcggatt atccccgtta ttggcatggt tatcagcttt ttttacgacc tttactgcta
     9001 gggatgagtt tggggagtat tcgaatgatt tacgcagcca ttctttttct gctgattgga
     9061 ttgacgagct actttttaac caagtgtagc gacatctatt taacgacagc cttgttgatt
     9121 tcgcttgcta taggaaatgc tgcaaccttt ttcttcagta tgcaattttc aaatatttgg
     9181 attttgactt tattgtctgt attactattt ttaacaaaac cacaactctc caacaccaac
     9241 caacaattat tcttgtattt ctttgtcatt ggttctttag ctaacttttt tgacttgttg
     9301 acaacaccgg tcatctcttg ggggatacct gtgatcatta tttattatgt aacaaataag
     9361 tatttaatgg ataaacgcag ttcattgagc aaacaagtag gcagttttgt ttttaccggt
     9421 atcttttggg gattaggcta tggtttgaca tgggtggcta aatggatcct atcatcgatc
     9481 atattgaaca aaaacattgt aaaagatgca atcaaccaaa tcctctttcg aacggaagga
     9541 aacgacaaat acccgttgca tcgattagag atgctgaaaa gcaatattag attgatgtat
     9601 ccaaaagtag caatattatt gctattgatt acttgtcttg tttttctcta ttttgcttat
     9661 cgtaaacgaa atagctggcg tccgttccaa cttctagttt tgtttgtttt agctgctgta
     9721 acgccgtata tttggtacaa tattttagct aatcactcac agattcatta ttggtttacg
     9781 tatcgtacgc aaatcatcac aagttttgct atacttagtg ctttcgcctt tttgatccct
     9841 cctgagaaat caaaagacga actaaattac ttttaagaat accgatgtac taattcattt
     9901 ttaaacataa aaactccagc tgtcagagat agccgga
//
LOCUS       DAZUMV010000101         9882 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000101 DAZUMV010000000
VERSION     DAZUMV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.102"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..3292
                     /gene="fss3"
                     /locus_tag="ACG8FK_002154"
     CDS             65..3292
                     /gene="fss3"
                     /locus_tag="ACG8FK_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS5097726.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3405..3719
                     /locus_tag="ACG8FK_002155"
     CDS             3405..3719
                     /locus_tag="ACG8FK_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5097727.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3732..4106
                     /locus_tag="ACG8FK_002156"
     CDS             3732..4106
                     /locus_tag="ACG8FK_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5097728.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4116..4451
                     /locus_tag="ACG8FK_002157"
     CDS             4116..4451
                     /locus_tag="ACG8FK_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS5097729.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4534..5874
                     /locus_tag="ACG8FK_002158"
     CDS             4534..5874
                     /locus_tag="ACG8FK_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5097730.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5952..6626
                     /locus_tag="ACG8FK_002159"
     CDS             5952..6626
                     /locus_tag="ACG8FK_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5097731.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6619..6783
                     /locus_tag="ACG8FK_002160"
     CDS             6619..6783
                     /locus_tag="ACG8FK_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097732.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6859..8001
                     /gene="dcm"
                     /locus_tag="ACG8FK_002161"
                     /pseudo
     CDS             6859..8001
                     /gene="dcm"
                     /locus_tag="ACG8FK_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            7991..8281
                     /locus_tag="ACG8FK_002162"
     CDS             7991..8281
                     /locus_tag="ACG8FK_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097733.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8540..9724
                     /gene="mobT"
                     /locus_tag="ACG8FK_002163"
     CDS             8540..9724
                     /gene="mobT"
                     /locus_tag="ACG8FK_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5097734.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9721..9858
                     /locus_tag="ACG8FK_002164"
     CDS             9721..9858
                     /locus_tag="ACG8FK_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS5097735.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3269 a         1669 c         2242 g         2702 t
ORIGIN      
        1 ataaacaaag atgttcaagc gagcatctta tttttttgtg cttaatttga aaggagaaca
       61 tttcatgtat aaaaaacaaa aaaagaaatt atgtgtatta actattttga gtttggctgt
      121 tcaagcaatt gttggagcaa ttgctcctac gattactttt gctgatgaaa ttacccatcc
      181 acaaacggta accgtagaac tagatttagc acatcaatat gctgttgaag gaacttttag
      241 tgatggtcgt ccaatgtcag aagtcactgt tccgcattat gcagtgtaca acggctttaa
      301 acaagatatt ttctgtattg aaccaggagt cccgatttac aatgagttca ctcctggcta
      361 tgagaaaaac ccgttaccag atatgtccga taaagccaaa ttagtttccg ttttatggaa
      421 aaaagtaggt acagatgcag atactcatat tgtggcacag aagatgatct ggcaagaagt
      481 taatgggtat acccttcact caataaaacg atcagatggt agtgccgtaa atatcgcagc
      541 cattgaggca aaaataaatc aagccattga cgattatcag aagaaaccaa ccttccataa
      601 tagtacggct aaaacagtat tagggcaatc gactactgtg acggatacga ataatctgaa
      661 tttgtcagag ttcgatgagg ttgtggaaaa tgccgcaaac attgattatc gtgtgaatgg
      721 taatcagttg ataatcaccc caaatgccaa ttcaaaagaa agtggtgtgc tgacgcttaa
      781 gaaatctgct ggtaccggaa cacctgtcgc ctataaaaag gctggccaac agactttaat
      841 ggccggggcg attgataaac ctaataccta cacaatcaaa attgatgtgg aaacggaggg
      901 ttctttaaag atcaaaaaag tcgataaaga atcgggtgat gttgtaccag gaacggtttt
      961 ccatttagac tttggaaaaa ctttacctgc aaaagacgtg actactgaca aagaaggcat
     1021 tgctacattg gatgagattc ctcatggtac aaaagtaacc attaccgaaa aatcagtacc
     1081 ggcgccttat acgattgata ccacccccat gactaccacc attaaagcag gcgaaaccat
     1141 ttacgtgact tcaaaaaacg cacgggaaaa agggcaaatc attctggata aaagcggagt
     1201 tgaaacaggg agtgatcttt ggaatgacaa ttattcctta gctggtaata cgtttgccat
     1261 tcgcaaagac agtccaaccg gtgaaatcgt tcaagaaatg accacggatg aaaacggtca
     1321 tgcggaaaca ccaaaagaga ttgctaatgc gttggaattg ggaacctatt atgtgaccga
     1381 aactaaagct agtcatgggt tcgtgaatac gttcaaacct gtaaaagtcg aattgaagta
     1441 tgccaatcaa accgtagccc ctgttaccag taacgtaaaa gggcaaaatc aagaagtcac
     1501 tggcgaaacc actttgacga aagaagacaa agatactggg gataaagcac aaggtaaggc
     1561 agtctttgag ggtgctgaat acaccttgtt tactgctaag gatggaaaag ctgttaaatg
     1621 gagtgaggct tttaaaccag agttagcgaa gggaacgaaa ggttctgatg aaacggtcac
     1681 tttgacttta gacgaaaaga accaagttgc cgttaaacat ttagctatca acgagtatta
     1741 ttgccaagaa acaaaagcgc ccgaaggata tagccttgat gaaacaaagt atcctgtatc
     1801 catcaaaaag gtggatgata gcgaaaaaaa tgccgtgatt acccgaaatg ttacggcaaa
     1861 agaacaagtg attcgctttg gctttgattt ctttaaattt gctggatcag ctgcgggcac
     1921 taccgaaact ggcttcaatg atctgacctt taaagtgtcg ccattggaag ggaccaatga
     1981 aatcacaggt gccgaagatg aagcgaccac agcttataac gagcaattag ggtttgatgg
     2041 ttatggcaag tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaagcccc
     2101 agaaggtttt caaaagatta cgccattaga aatccgttct acttttaagg aaaacaaaga
     2161 agactttgtg aagagtgagt atgtctttac cattaccgaa caagaccaaa aacaaccaat
     2221 taaaacggtg accgttcctt acgagaagct gacgaacaaa gcattttctg ttagtttgaa
     2281 ccgtttaatg ctctatgatt tgcctgagga agaagatagt ttaacttctc ttgcgacttg
     2341 gaaagacgga aacaaagaat taacttctct ggattctacc gagctagttg ataaattgag
     2401 ttacaatcta catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga
     2461 tgcgacaaaa gctgcccaag aaaaagatga aaaagccaaa ccagtggtga ttgcagaaac
     2521 aagcgcaacg ttggcgaaca aagagaaaac aggaacttgg aaaattcagc ataaattaac
     2581 cgccgaacaa gttttaaata aaactattgt cttattcaac tatgtgtatg aaaataagga
     2641 agcttttgaa gcaggcgata agccagtagc gaaggatgtt agcttgaata accaagccca
     2701 aactgtcagt tgtacagtgg aacaccatgt ttctatccaa acaaaggccc acctagaaaa
     2761 cggttctcag acctttactc atggtgatgt ggtggatatg tttgatgatg tgtcgattac
     2821 tcatgatgta ctggatggct caaaagaagc tttcgaaaca attctgtatg ctttattacc
     2881 ggatggtaca aacaaagaaa tttggaaatc tggcaagatt gattatgaag taaatgataa
     2941 agaattcacc aaaactgtgc ttgcgaaaaa agtagatacc ggaaaatatc cagaaggaac
     3001 caagtttact tttgcggaaa tcaattacga taaagatggg aatatcaatg gaaaacacaa
     3061 tgaagatttg aaagaaaaat ctcaaacctt aacaccaaaa gaagttccaa ccatactaag
     3121 tacgccgaaa caaccggaaa caccaactgt tccaagtggc tctcaagaat ctagtcccac
     3181 agtgaagacc ttccctcaaa ccggggagaa aaattccaat gttttactgt tcattggttt
     3241 tactttgatt tttgcgacgg cgggctatta tttctggaat cgccggaact aaggtgatga
     3301 gatgatacaa gtaaatccta agaaccaacg tgcgcgaccg cctcctaaaa aagtagtcgc
     3361 gctatttttg tatccaaaac taacaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     3421 tcgtgccaga tatggctgaa acctttggaa agttaagtta tgccggtgaa ggagaaatta
     3481 taacagaagg atatggacgg aatactacgg tgattggtcg cagttatcat ttgtattcca
     3541 gtaagcaacg ggccgatgat attgaggtgg tagtagctgc ggaagccggt gaaaaggatt
     3601 ttgatcaaga ccaaccgcta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     3661 ttgagaatcg tgggtttact gattacgtgt tgtatgttga tgatttagtg aaagcatagg
     3721 aggaaaagaa gatgagatta acagaaggta ttgtcgtaga ttcaggttta acatttggga
     3781 agttgcgttt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacaattg
     3841 gtaatgaggt aaaagaacga acctataatt taaaatcctc tgcacaaggg cgaatgattc
     3901 aagttagtat cccagctaat gtacccttac gcgagtttgc ttatgatgca gaggtagagt
     3961 tggtcaatcc aatcgtggat accgttgcca attatgtttt tcgagaagga actaccgtta
     4021 attggttcat taaggcggat gatttagtct tgaagcggca gccgaatcaa ggaaatccta
     4081 cgaaccaaaa tgaaagaaag aaataggtga agattatgga aattaatgtt gaacatattt
     4141 tagattgtct cgatcaatat ggaaaaggcg agctgactga ggaacaactc gttaaagcat
     4201 taacctctga tgaaaaaatg tttctgatta tacatcaagg tttacttgga gaaaatagcg
     4261 acagtgaaga agactctgat gtactagact ggttaggaga agaagagtct ttctttatag
     4321 ttgtcgaagt aaatgaaaac ttatgtcggc aggcagaatc cattttggaa gaaattggtg
     4381 tggagatgcc ggatgctatt gaaggctttt taaatcaatt ggttgaaacg aaacaactcc
     4441 cctcctcgta gcagtaaatg actagcataa ctttttgaaa ttgcctaatg aaatcctatt
     4501 gggctttttc gattggaggt ggaaaccatc aaaatgtaca aaggacatcg catacgcgca
     4561 ggagatcagc acttggttta tcactttgtt cttggctggc tactcgctct gttcatcggt
     4621 tggatgagcg tcttttattt tcaagagttt agacaatttg atatttctaa gctatcactt
     4681 tctactattg aaatcgtttg gtctataaaa gatttagtat gtctgcttgg aagtctcgct
     4741 ttttcaggag ctatgatatt gctttatatt cacttttttc ttgatcattg gcgaagcttg
     4801 tggcatcgac aaaagctagc tcggatgatt ctagagaacc attggtatga agtgaaacaa
     4861 actcaaagtg aaggcttttt caaagatttg aatagtagcc gaaccagaga gactatcagt
     4921 tacttcccca aaatctgtta tcgaatgaaa gatggattac tgtctattcg cgttcaaatt
     4981 tcacttggga aataccaaga tcagctttta aaattggaaa agaagttaga aagcggactg
     5041 tattgtgagt tagtagaaaa ggaactcaaa gactcctatg tggagtatac tttactgtat
     5101 gatatgatcg ccaatcgaat tgggatagac gaagtagtgg cagaaaatgg gactctgcga
     5161 ttgatgaaaa atcaagtatg ggcttatgat tctttacccc atatgttaat cgctggtggc
     5221 acgggtggag ggaagaccta tttcctcctc accattatcg aagcactatt gaagtcagat
     5281 gcagaattgt ttatcctcga tcccaaaaat gcagatttag ctgatttagg tacggtgatg
     5341 cctcatgttt attcacaaaa ggaagaaatt tctgcttgtg tggaagattt ctatgaacgt
     5401 atgatggctc gtagcaaggc aatgaaagaa atgtctaact acaaaacagg agagaattat
     5461 gcctatcttg gacttccacc caacttttta atctttgatg aatacgtggc ttatatggag
     5521 atgttaacga cgaaagaaag tgcggtgatt ttgaataagc tcaaacaaat cgtgatgttg
     5581 ggtcgtcaat ctggtttctt tctgattttg gcttgccaaa ggccggatgc gaaatattta
     5641 ggagacggaa tccgcgatca attcaacttt cgcgtggctt tgggtcgtat gagtgaactc
     5701 ggatattcca tgatgtttgg ggaggtcgaa aaaaacttct ttatgaagca catcaaaggt
     5761 cgaggttatg tggatacagg tggcagtgtg attagtgagt tttatacgcc acttgtacca
     5821 aaagggtatg atttcttaga ttcaattagt aaattagaaa agatgaatga ttgagtgatc
     5881 ttcaatactg actttatact tcaagtaacg tataatactt ttatatagta ggaagaaagg
     5941 ggataattaa tatggaaagt aatgattctt atttagagta tgcaaaagca aataaggata
     6001 agtttatttc ttatattatc aaagggaaat cccccgaagt tggacgagac gcagttttta
     6061 tggcagggag tcctggggct ggaaaatcag aggttgccat tggattagct gagaattatg
     6121 ataaccatgt gattattgat gcggattact ttcgcaccca gtttcctgat tataatggaa
     6181 aaaactctag tctctttcaa aaagcatctt cttggttggt agagcaggca tttagatatg
     6241 tcttagatca tggctattct tttattctgg atgcaacttt tgcaatatta agtgctgaaa
     6301 aaaatatctc gcgggcgttg aaaaaacatt ttcgagtgac gattttctat gtttatcaag
     6361 accctaaaat cgcttggaaa tttacacaag aacgtgaaat tgcagaggga cgtcatgtgc
     6421 cgaaaaaggc atttattaac gcttttttca aatcaagaga gaacattgca aaagtaaaga
     6481 gtaaacatcc agatgtattg cttcatatca taataaaaga ttatacgaat aatatttccg
     6541 aagttcatta tgctacggat aatctagaat tatttttacc actagggtat agtaggaaag
     6601 atttggaggt tcaattacat gactgagaaa gaacaagtaa cgaaaattgt aaagaaatac
     6661 aataagagca ttgcggattt atcggaaaat gctactgcaa aagagttcaa aacggtgatt
     6721 aaatatgtag ccgatcaggc caatgaaaaa caacggaaat tagttggatt aaataaaaaa
     6781 tagtaggaca aattaataat gtatgagcca agagcaaaat gttcttggct tttttctttg
     6841 caatgaatga ggtaatgtat gagattttta gatttattcg ccggtattgg tggctttcgt
     6901 ttaggtatgg aacaggcgag ccatcattgt atcggttttt gtgaaattga taaatttgct
     6961 agacgtagtt ataaagcaat tcatgataca agtaaggagg tggaaatgca tgacatcaca
     7021 agtgtatcag atgagtttat tcaatccctc ggaccagtgg acatcctttg cggcggattt
     7081 ccgtgccaag ctttttcaat tgcgggaaag cggcaaagat tttccgatac tcgaggtact
     7141 ttattctttg aaatcgctcg gttcaccact cttctccgac ctaagctttt attccttgag
     7201 aacgtccgag gattgctcaa tcacgaagga ggggctacgt tcgagacgat cctccgaacg
     7261 ctggatggat tggggtatga cgtggagtgg taggtgctta actcaaaggc ctacattccc
     7321 caaagccggg agcggatatt ccttatcgga cattctcgag acgcatgtac cgaacaagta
     7381 tttcctatca ttggaccgtc ttcaacatct gatcaaaaca tcagaaattt ggtaaatatc
     7441 aatccttcca atcgaggaat gggtggacaa gtctatggtt cagatggcgt agcgccaacc
     7501 ttaacaggtg atgaaggaat caaaatcgca ctgccagcaa gggatggaat ctctgtagca
     7561 ggactattac cgggaaagtt tgaacaaggt aatcgagttt atgaagttac tggaaccgcc
     7621 cccaccttgt caacgaaaca aggtggtaca aaaatcatga ttcgaaaaaa tgaaacagaa
     7681 tatcaagagg tgaagccaga agacagtgtg aatctggctt ttcctaattc cacaagtcgc
     7741 cgaggtcgtc tgggaaaaca gagtgttcat accttactaa caggagacca acaagcgatc
     7801 gtcacggatc agtatcaaat tcggaagcta acacctagag agtgttggcg acttcagggc
     7861 tttcccgatt gggcttttga tcgagattct caagtcaatt cagatagtca gttgtataag
     7921 caagccggaa actctgtaac agttccggtc atttttgata ttgccagaag attaaaagag
     7981 ttggatgtac atggtttatg atgagttgat tggagaaatt tattgggtga ttggaaagat
     8041 tcagtcagac ccagaattag aagaacaatt gcgacggtta aaatttgata ttcgcaagaa
     8101 tggtgtcaaa gtcgctggcg atccatattt aatgaatgag gagacggatg ctcgaattga
     8161 aataaatcaa gtaattgcag aatttgagcg gattgcggac caagcaaaag aaccagatat
     8221 tcgtcaatac ttgttagaaa tgaaagcaga gttggaagta aatggtatta cagatgagta
     8281 acctaaacca acacaactga ctgttaggcg ctgaccgcgg aggattcgcg aagcagaaat
     8341 cctccgtggt gctgtgcctg ttcatggacg gcgttagccg accatgatgt ctcacccccg
     8401 tatctaacag gggggtacaa aaacacaaaa aataagtaag caaccattaa gaatgcttgt
     8461 tgtatcaaag atagtaagta atttgtatag gtgttaactc atttgaaaaa gttaacacct
     8521 tgaatttgga ggtgagaact tggcacaacg gaatttagat tatcggctat taaaagaccg
     8581 acgcaacgaa tacggcgttt cacaaaataa attggctacg gcttgtggac ttagtcggcc
     8641 gtacttaaat cagatagaaa atggtggcgt aaccgcatct ataaaaacca tgaggaaaat
     8701 ttttgaccaa ctggaaagct ttaatcccga tttgcctttg acgttactgt ttgactatgt
     8761 aaggattcgc tttcccacaa cggatgcacg aaaaattatt caagaaattc tccatttgaa
     8821 atttgagtat atgctccatg aagattatgc cttttactcc tatcaagaac agtatgtcat
     8881 gggtgatatt gtggtgatgt tgtctcatga agaagataaa ggtgtgcttt tagaattaaa
     8941 ggggcgaggt tgccgacaat ttgaaacttt tttactcgct caaaagcgta gctggtacga
     9001 ctttttcgag gattgtctaa aggccggtgg cgtgatgaaa cgcttggact tggcaatcaa
     9061 tgatcgagtg gggctattgg atatacctga gctaacgaaa aaatgtcaga aggaagaatg
     9121 tatctccttg tttcgtacct ttaaaagtta tcgttccggg gaacttttaa aggctgatga
     9181 aaaggacggc atgggcaata ccttatatat cggcagtctc aagagtgagg tttatttctg
     9241 tttgtatgaa aaagactatg aacaatatat caagttaggg attccattag accaaactga
     9301 gacgaaaaat cgttttgaga ttcgactgaa aaatgaccgt gcttttcatg cgattcaaga
     9361 tttgttaaaa ggtcgtagca ttgagagcac cacgttttcc attatcaatc gttacttgcg
     9421 atttgcggat aaagtagaag gtaaaaggcg aaccaactgg cccttgaatg aacaatgggg
     9481 gcgttttatt ggacggaatc ggaaagagat tcaattaact tccgagccaa aaccgtatac
     9541 cattgaacga accttgaatt ggctaggccg acaagtcgcc cctacgtgga aaatggctaa
     9601 ggagttggat cgattgaatc ggacaaccta tatccaggat atggtacaga atgcacgctt
     9661 atcagaccga cataaaaaga ttttggagca acaaagtatg gcaattgaaa atttgattat
     9721 atgaaaagag gaatcgtcat gggaattgta aaagatattt ttttggtatt tggtggcagt
     9781 atgcttggtg ttactgtgat gtgcttgatg aatgcaagtg cagcagcgga tcgagcaatg
     9841 gaaaagaaag gaaagtgaca agatgaattt cggacaaaat tt
//
LOCUS       DAZUMV010000102         9873 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000102 DAZUMV010000000
VERSION     DAZUMV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.103"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(235..1425)
                     /locus_tag="ACG8FK_002165"
     CDS             complement(235..1425)
                     /locus_tag="ACG8FK_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFS5097736.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(2297..3928)
                     /locus_tag="ACG8FK_002166"
     CDS             complement(2297..3928)
                     /locus_tag="ACG8FK_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS5097737.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3942..5615)
                     /locus_tag="ACG8FK_002167"
     CDS             complement(3942..5615)
                     /locus_tag="ACG8FK_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS5097738.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5628..5945)
                     /locus_tag="ACG8FK_002168"
     CDS             complement(5628..5945)
                     /locus_tag="ACG8FK_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5097739.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5970..7370)
                     /gene="lacG"
                     /locus_tag="ACG8FK_002169"
     CDS             complement(5970..7370)
                     /gene="lacG"
                     /locus_tag="ACG8FK_002169"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS5097740.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7607..8392
                     /locus_tag="ACG8FK_002170"
     CDS             7607..8392
                     /locus_tag="ACG8FK_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5097741.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8395..9087
                     /locus_tag="ACG8FK_002171"
     CDS             8395..9087
                     /locus_tag="ACG8FK_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5097742.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9361..9732)
                     /locus_tag="ACG8FK_002172"
     CDS             complement(9361..9732)
                     /locus_tag="ACG8FK_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS5097743.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2983 a         2145 c         1728 g         3017 t
ORIGIN      
        1 gaatcattct cacctataat agtatttgtc tcattatcga atcgaaagat ttcggattca
       61 taatttctaa aatttcgaat ttgaagagta gatataaaca taagcagatc tcctaactat
      121 ttaattaata tgattatata gtaagtttat cataaattat gttagtaaaa gttctcatta
      181 taagggagcg tcaataattt tgtgtaaatg attcttccct actgcagatt gtttctaatt
      241 ctcaatcagt tggcttagca tttgttcgag ctcgccttcg gcctgttgga aacctttatg
      301 actgcgacct aggaattttt ggttgtaagt atcaaaagac gagactaaga aacgttccat
      361 cgactcttca ttttggaact gttccttgcg gtggctgtat ttcttgattt gtttattaaa
      421 ggattcaatc aagttcgtag aataaatact cttgcgtatg gccaagggga aatcatagaa
      481 agtgagcaag tgatcgttcg aaagaatcga ttcaaccact tttggatagc tggttttcca
      541 tttttccgca aaagtaccac gtgcttccaa cgccacctct ttagatgagg cttgatacac
      601 cattttaaaa tcatcacaga cttccttacg atcatcgaca cgcactttat gactgatatt
      661 acgggaaacg tgtacacaac aatgttgaaa acgagctttg ggatagaacc gactaatcgc
      721 tcctaccatc ccctttaagc catccgtgat gaacaggagg acatttttca aaccgcgctc
      781 ttgaaggtcc aataaaattt cctcccaaat cgtgatggat tcagtcggtg caatcgcata
      841 gctcaatact tcctttgatc cgtccgggcg aatgccaact gcgatatgaa tagcttcctt
      901 ggcgacggtt ttccgcttta acggaatata cgttgcgtcc atataaatag cagcataacg
      961 gtcatgaagc tcccgcccat taaacgccgt tacctcttct gtaaatgatt ttgttatatt
     1021 ggacatggtt tggggcgtgt agtaatgccc atacattttc tcaatcaagt ctgcgatttc
     1081 cgacatggta ataccttttc ggaagaggtg aatgacggtc tcctctaacg tgtcattcgt
     1141 ccgtctataa gcaggaacag tctgttgctt gaactcacca ttgcggtcgc gcggaatctg
     1201 gagatgaagt tccccatact cggtcttgac cgtacggtca taggagccgt tacgagaatt
     1261 acccgtgtta aaaccaatgc gatcatactt ttcgtaatct aggaaagccg ttaactccgt
     1321 tttgagaagc gtattgactg ctttctccaa atggacacgg aataattcat tcaaatcgcc
     1381 tttgttcgct agagtcttta gaatttctgt agtaaaatcg ttcataggga agtcctctct
     1441 tctgtgaatg tgttgtggta actctattct acagaaggga cttccttttt tctatttaca
     1501 caaaatattt tacactctcc attataacaa ttgggttaaa aagaaaatag atagtattct
     1561 acctcatatt tatttgaaag taatatataa aagacgttca aattcgaacg cctctcgtta
     1621 accaaatatt ggattcagaa tctagttttc atatataaac agagtatgat taggtgcaaa
     1681 aattgcactt ttattgcact tttattttta tttatagtgt tttactgttc tgtatattgt
     1741 tttaactccc ttgatagtat cgcattctgt tttatacact ttacgttgaa aacagagtgg
     1801 gaataaagag ccaacataat atggaggggg aatccctata aatcaacatt cttcaatagc
     1861 taaaatgctt gattttcatt aaaaataaga tggttcccta ccaaaagaga ctataatata
     1921 agttaatttg tttcattctt atcataaaaa gaagttctcg aaaaacgaac tgttttccga
     1981 gaactttttt ctatccatct aagtgctcaa atcatattca cgacggaatt ctaaggttac
     2041 cctttcttaa acaaacttta tgttatctgc acaacttttt gcaatttttt cattgcttct
     2101 tacgaaatcg ttgaactctc tttagcataa tacaaagtgt agatgaagtc tggtgcttta
     2161 ctcgttttgg taaaaatgac tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc
     2221 aggttcgatt cctgccttct acgctaacga tgtggctgaa gggaacctcc gatcttgaat
     2281 cgaaacgatc cgcagtctat tgtttcacca atgaaacaaa atcaacgttc gtccaagcat
     2341 ttcctgaagc cttgctataa accccaattt cacaaacacc tgttgtaatt ggaatgtctt
     2401 tgattgtaat ttttgtccat tcacttgttt taacaaccgc agtatttagt tccgctccac
     2461 cataattttt gatatataac tgattagtat caaactctcc cccagacatt acataggcac
     2521 tcaatgtgta agttcctttg cttatgccac taacattttg ataagctgat gcctcgtatg
     2581 cttgatcctg ccaaaaagtt agtttgtagt ccccaaaaaa agtgcccgct tccgttttga
     2641 tcgtaccctt agcggttcct tgttgaaacc aggtttgcca attggaagga ctggtcgttg
     2701 gtccatcgct ttcgaaactt gcatttttaa ctagatttat ttgcgcccct gcttgtttaa
     2761 acgcacgcaa gcttggcaag gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt
     2821 cgtttcccat agtggattga acactgagat aattcatacc tgcttgtgtt gcccaactca
     2881 catttccgtt gtaccaaaga ggttcccaat agaagaagcc cgccccgcga ttattcggta
     2941 ctgcctgaat ccgatcggca agatccagca aatactctgc ttgtcctgtc ggtgttggtt
     3001 catatccccc cgcagctgct tcggcttcac caaaagcatt tggcatagta tcggcattcg
     3061 ctgttgtatg cccataagct gtttcaacta cgatcacttc tttaccatac cgttggctga
     3121 tcgcattcat attggtttga agttcattca ttgaaccgtg ccaatacgga taatacgaca
     3181 ctccaattaa atcaaatgac acacctcgac tcgtgatttc atcaaaccac caagtaaatg
     3241 ctcctgtatc gccaccatca gctaagtgga gcattactgg agttgtttta ggttgagtgt
     3301 ctcgaacagc ttggatacca gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc
     3361 cttctcccca agacttgcca taaggccaga gcattccgct attcagttca ttgccgatct
     3421 gaaccatatc aggataaacg tccagctgct tcatttccga cagaacgttc tttgtatagt
     3481 cgtacaatgc cgtattcatt tcttcaaagg tcagaccttg ccatgcttta ggaagattct
     3541 gtttcccagg atcaacccaa aaatcactgt agtgaaaatc cagcagcacc ttaagtccca
     3601 gattttttgc tcgttttgca aggtcaatcg tcgtttccag atcatttgtc cctgctccat
     3661 aactttctcc attactatta taaggatcct gccacaaacg cagtcgaaca tagttgacac
     3721 cattattttt taaaatctct aaggcatctt ttgaaatatc gtcttcataa tattttgcac
     3781 cagatttttc catatctgac aggatcgaaa tatctgctcc gcgaataaat tcgctggcag
     3841 atgcttcaac agtttttgaa acagccccca aagcccctaa cacagctgca aataaaacag
     3901 ctttacgcaa ccaaattccc attttcatta tggctcctcc tttattgaaa ttggttcaga
     3961 ataaactgaa cagctttttc aggatctctc gttaatccga tatattctgc tccttttgtc
     4021 gcaaccagct tgattcctaa gcgatcggtg tcttctttga tatcatcata gtaagaattg
     4081 acttgcggtg ctaagaccac catatcaaaa tcctgcagca tttcgtaatg agaaccataa
     4141 gcacccgcca ttgcactgat cggtgctttc gattcagatg cacctttttt taaagcatta
     4201 gctaacatcg cacttgtccc tgcgccagca caaagtacca acacattgat ttctttattt
     4261 gcatcgctga taacttgact cgctgccaca ggtacttcct cacgtacatc ggcagaagca
     4321 ccttcctcgg ttgcgatcac ctctgctttt tgtgcttctt tctctactaa gacagcatcg
     4381 tatgctttgc aaaatggtag atagatcagg aagtcaacga cgattagaac cgctgctaga
     4441 acaaaagcta agatacccat gcccgttcct agaaccaaac cgatcggtgc tggagttgcc
     4501 caaggcaaaa cgtacataaa gctgttcatg cccaatacat cgacaaaaaa cttgaacagg
     4561 caaacgttta caacaggtgt taacaaaaat ggaataaaaa aataagggtt caaaatcatg
     4621 ggtgccgcaa atagcaatgg ttcgttgaca gcgaaactta ctggaataaa ggaagctttc
     4681 ccaactgcct tcaactgctt agacttagca aagagtagga agataaaagg aacaaccaat
     4741 gttgccccag ttcctcccat agttcctaca aaattcccta aacccaccgt cagtacttta
     4801 gaagcttgtt caccggaatt gaacaattgc agattggttt ccacattggc atagataatt
     4861 gccgcaattg ccggctccac gatcgacggt ccatgaacac cgacaaacca aaacagcgcc
     4921 atcgctcccc agataatagc gattcctaag tacccgtcag ctgctgtaaa tagcggctgc
     4981 agcaccgtaa tgattgcttc tgcaaatgag atttggaaga tttgtcgaat gcctaaatca
     5041 attaaaacgg ccgcaaatac tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa
     5101 atcgttccag gaacttcttt aggcatttta atggtaatat ctttcagcac acaaaactta
     5161 tataaattaa ctgttaaaaa tgcagcgaca aaggaagcca gtagtccctt tgtccccata
     5221 tattccgttg aaaatccacc ttcaatcgga gagacgctta acaacaggaa acttaccaca
     5281 gaggccagca tcacagaggt ggtgttgatt actttgtttt tcggtaagcg acgattcatc
     5341 gaatcagcca agcagcgagc agtggttgct gaaacgagta accctacaac acccatcgaa
     5401 taattataga ctttccatag ccatgtggag acagattcag gccattggta accaaaaatc
     5461 tcaggaaccg cagcaatcaa aatgaaaatg cttgaaaata aaatagctgg catcgctgtt
     5521 aaaaatccgt cacgaacggc tcctaaataa ctattacgtg agattttttc aaaaaatggt
     5581 ttcccttttt cgatttgttt aatgattcca tccatagcgt tcttctccta tccttgattc
     5641 tgtctgtaca gtacaataaa gtcttgaatc agatctctca gcaataaagt ggtcattaaa
     5701 tgatcctgcc catggataaa aatgaatccc atctccatct ctttcccaga tgcttcttcc
     5761 gctaaaattt ttgtttgcga attatgtgca agggttaaat tttcgtcggc ttctttcaac
     5821 gccgaatcaa catgttcaaa gtttccttgt cttacttctt tcaataattt caataatgta
     5881 ctgcgagcgt cgcctgaata tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta
     5941 ttcatctttt cttcctccat tatcacttat taaataatcg ttttggtttc actgaccaac
     6001 ttgtaccagt aagctgattc ttttgggtaa cgcttctggg tttcaaaatc aacataaaac
     6061 aagccatacc ttttgttata cccatttgtc catgagaaaa gatccataag cgaccacaaa
     6121 aaatacccct tcacatttac cccggctgtt atcgccttgc ttaaggattc caaatagact
     6181 ctcaaatagt cgattctcgg ctgatccata ataatgccat cttcaaactg atctttatac
     6241 cccatcccgt tctcagtgat atagattttg ttgtaatgtg ggtaatcgct tttaattcgc
     6301 agcaacaagt catatagacc ttctggataa atcagccagt cccaatcagt tcgaggaatt
     6361 ccttctttat aaatacgttc tccgattccc ttcactttat aaacagaggt ccctttttca
     6421 cccgtaccgt tatgatggat tgcattttct ccatcgtaag ctttgacaaa atggcattgg
     6481 tagtgattga tccctaagta gtcattacgc gtggatgctt ttttcaattc aacgaaatct
     6541 tcttcaggaa aatgatagct ggcctgattt gcttcacata tctcatcaag agcagtcaag
     6601 gtttcagttg aataatagcc taaataggta gcatctaata agaaacggat cgacaatgca
     6661 tcgtctaaaa aggcagcatg cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct
     6721 aatgagtgaa ctacaccaat ttcacccgga agttcgtttt ctttgaaata gttcactaca
     6781 cgggcatgag caaccatcat attatgaaga caggcgacga tctttgtaaa atcatatttg
     6841 attcctggtg ggaaaacacc taacaaatat tgattggttg caactggata gatttcatta
     6901 aaggtactcc acaccttgac ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca
     6961 aatgcttcta ttgtttctcg attcaaaaaa tctccatggt caaacaaacg tttaggggta
     7021 tcaaaatgat gcaacgtcac aaacggcgtt acgtttcttt tcttacattc ttcgaacaca
     7081 cgatgataaa aagcgattcc ttctggattt ggtttcccag ctccatcagg aaaaatgcgg
     7141 ctccaagcaa tggataatct tagaccattt acaccaaaac gttcacacaa ttcaatgtcc
     7201 tttgcatatt gatgataaaa atcacttgcc ggatcgggac tgaatcgccc ttgttcctct
     7261 aaaaagtcat cccaagcaac agcgcctttg ccaccttcct tagtggctcc ttcaacctga
     7321 taggctgctg tggctccgcc aaaaataaaa tcttctggta atttaagcat gtacatctct
     7381 cctcaaatct ataatcagtt ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa
     7441 acatttgtca acaaaaacaa acacaaataa tcataaaaac cagaaaacag caataaaatc
     7501 tattgtaaca aacataaaaa ccaccctaaa tgtttacttt tgttcgaatt agattgactg
     7561 ttgtccgttt atatgataga ttatccttaa attagaggtg aagacaatgt tgaaaaaaga
     7621 aagattgctt actatcgtag aaatggtcaa taaaaaagga atccttaccg ttaatgaaat
     7681 catcaatcaa ttagatgttt ctgatatgac cgttcgcaga gatttagatg aattagaaaa
     7741 atcgggcaag ctgctgcgtg tccatggagg ggctcaaagt ctttcctaca ctttagatca
     7801 agaactttct cacactgaaa aagccactct gcagattcaa gaaaaaaaag aaattgctgc
     7861 cacagcagcc tccttgattc acgagggaga aacgattttc ttaggaccag gtacaacaat
     7921 cgaactattg gcgacgcatt tactgaacaa gaaaattcgg atcattacaa ataattatcc
     7981 agtttttgat attcttgctc aagcagatgg accagaaatt ctactgattg gcggtgattt
     8041 ccggaaaaat accggcgcat ttgtcggacc gatcaccaac gacaatttaa gaagactaaa
     8101 gtttacaaaa gcatttatca gttcaaatgg cgttcacaac gaagcgatct ctacttacag
     8161 taccgaagaa ggagaagcac agcagatcgc tctaaataat tcccgtaaga aatatctact
     8221 cgttgataat aaaaaattca atcgagatga tttttatgtg ttttataatt tgcatgattt
     8281 cgatctgctc attacggatc gccagatctc gcgggaggtt caagagcact atgcacaata
     8341 tgtcgatctg caaattgctg attcaaccga aaataccact ataggagcgt aactatgaaa
     8401 gggattatat ttgatttcaa cggaacaatg tttttggact ctcatctgca tgaagctgct
     8461 tggctgcata tgatacatga tcatacaaaa gatggtttat cagacgagga tattttaaaa
     8521 aatattcatg gtcggacgaa tacggagatt ttgaaacatt tcatttcaga gcatttgacc
     8581 gcctctgaga tcgccgtatt gtcagaagaa aaagaaagct attatcgtat gctttgcttg
     8641 caaaatgaaa acgagcttca gttgacaaaa gggcttgaag cgactcttga ccagctgact
     8701 gaccaacaga ttccattaac gattgccacg gctactgtca aagagaatgt tgctttttat
     8761 tttgatattt tcgatttagc ccgctggttt gactgggaca agattgtcta tgacgacggc
     8821 tctttccctg ggaaaccgca accggatatt tttcttaaag cggcagacaa actgagctta
     8881 gctccggctg attgtcttgt tatcgaagat gcttactcag gattacttgc tgccaaacgt
     8941 gcggggatcg gaacaattat tgcaattgat ccattcggta aaaacaaaac agtatttgaa
     9001 caggcacagc taaataaaga tggaaccatc aaagatttta ctggtttttg gacagagcac
     9061 ctgtctgcca catccgttaa ttcttaaacg attcaactaa ctaccaaaga aaagcaactg
     9121 aaatgcacac ctccttttat aaaaaactgc cagtgaaacg acatgaaaga tacactgcta
     9181 tatccttagt gaaatatagc agtgtatctt tgactttttc attcttttaa ccatatgacg
     9241 cgttcatctc tatctataat taacattaaa aatagtgttt tcacattatt tctcaaacca
     9301 atcttaaaaa tcatcaggag tacttttttg atatactttt attaatgctg ggcgtaatgg
     9361 ttaaaagatt tccactagtt cctctccacc aagctgagtt gaaaaatgct tatctgtaaa
     9421 caacatagac aaagaggaaa taaacgtttc cgtatctttc tctgtcccaa ggatattaat
     9481 taattttcct actaggtaag tatcattaaa cttaagatct gccttcacat caaaagtttt
     9541 gataaaagct tttatcatgc gtacaatttt gtctttatcg ccataattga tttcaccact
     9601 catatcaata tagtcactaa tccctgaaga aataatggca actgcttctt ctttcgcctt
     9661 atcagataat tcatttaatg actgggattg attgatgact tcttgagttt tatctgttac
     9721 gtaatctctc attatcctat cggcatactc cgcaaagtaa tcttgaaaaa attcttctac
     9781 agggtaacca acttcgtttt cccaaccagg agaaatttca ttatcagtac ttattaaatc
     9841 tattaaaaac caacccgttc tctgagtgtt gat
//
LOCUS       DAZUMV010000103         9871 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000103 DAZUMV010000000
VERSION     DAZUMV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.104"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..684
                     /locus_tag="ACG8FK_002173"
     CDS             205..684
                     /locus_tag="ACG8FK_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS5097744.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="ACG8FK_002174"
     CDS             717..2945
                     /locus_tag="ACG8FK_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5097745.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="ACG8FK_002175"
     CDS             3074..4717
                     /locus_tag="ACG8FK_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5097746.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="ACG8FK_002176"
     CDS             complement(4790..5335)
                     /locus_tag="ACG8FK_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS5097747.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="ACG8FK_002177"
     CDS             complement(5358..5843)
                     /locus_tag="ACG8FK_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5097748.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="ACG8FK_002178"
     CDS             complement(5833..6645)
                     /locus_tag="ACG8FK_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS5097749.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="ACG8FK_002179"
     CDS             complement(6784..8316)
                     /locus_tag="ACG8FK_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS5097750.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="ACG8FK_002180"
     CDS             complement(8425..9456)
                     /locus_tag="ACG8FK_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5097751.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3282 a         1833 c         1900 g         2856 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcag cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aagatttatt c
//
LOCUS       DAZUMV010000104         9822 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000104 DAZUMV010000000
VERSION     DAZUMV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.105"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..397)
                     /gene="rplT"
                     /locus_tag="ACG8FK_002181"
     CDS             complement(38..397)
                     /gene="rplT"
                     /locus_tag="ACG8FK_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS5097752.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(450..650)
                     /gene="rpmI"
                     /locus_tag="ACG8FK_002182"
     CDS             complement(450..650)
                     /gene="rpmI"
                     /locus_tag="ACG8FK_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS5097753.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(722..1246)
                     /gene="infC"
                     /locus_tag="ACG8FK_002183"
     CDS             complement(722..1246)
                     /gene="infC"
                     /locus_tag="ACG8FK_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS5097754.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1290..1415)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1566..2198
                     /locus_tag="ACG8FK_002184"
     CDS             1566..2198
                     /locus_tag="ACG8FK_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5097755.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2200..3105)
                     /locus_tag="ACG8FK_002185"
     CDS             complement(2200..3105)
                     /locus_tag="ACG8FK_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5097756.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3190..3849)
                     /locus_tag="ACG8FK_002186"
     CDS             complement(3190..3849)
                     /locus_tag="ACG8FK_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5097757.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4165..5109
                     /gene="mvk"
                     /locus_tag="ACG8FK_002187"
     CDS             4165..5109
                     /gene="mvk"
                     /locus_tag="ACG8FK_002187"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS5097758.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5102..6079
                     /gene="mvaD"
                     /locus_tag="ACG8FK_002188"
     CDS             5102..6079
                     /gene="mvaD"
                     /locus_tag="ACG8FK_002188"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS5097759.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6092..7177
                     /locus_tag="ACG8FK_002189"
     CDS             6092..7177
                     /locus_tag="ACG8FK_002189"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS5097760.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7174..8238
                     /gene="fni"
                     /locus_tag="ACG8FK_002190"
     CDS             7174..8238
                     /gene="fni"
                     /locus_tag="ACG8FK_002190"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS5097761.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8487..9290)
                     /locus_tag="ACG8FK_002191"
     CDS             complement(8487..9290)
                     /locus_tag="ACG8FK_002191"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS5097762.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9310..9633)
                     /locus_tag="ACG8FK_002192"
                     /pseudo
     CDS             complement(9310..9633)
                     /locus_tag="ACG8FK_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            complement(9666..9803)
                     /locus_tag="ACG8FK_002193"
                     /pseudo
     CDS             complement(9666..9803)
                     /locus_tag="ACG8FK_002193"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         2881 a         2070 c         1900 g         2971 t
ORIGIN      
        1 ttttcgttat cttctttcct ctttataatt gtttagctta tttagccaaa gcttcttttg
       61 cttggtcagc caatgcagta aatgctgctg catcgttaac agccaagtca gctaacattt
      121 tacggttgat gtcgatttca gcaactttca aaccgtgcat taattttgag tagctcaagc
      181 cattcatacg agctgctgcg ttgatacgag caatccacaa tttacggaag tcgcgtttct
      241 tttgacgacg atcgcgatat gcgtagttgt atgaattcat tacttgttct tttgctgttt
      301 taaataaagt gtgttttgaa ccgtagtaac cttttgctaa ttttagcact cttttacgac
      361 gtttacgagt tactgttcca cctttaacac gtgccatgta taattcctcc taataaaaat
      421 aaatcttcag attctgttat ttgtacttct tatttcattt ttgctagttg ttggcgaata
      481 cgtttgaagt cgccactaga aaccatgctt gctttacgca attgacggcg ttgttttttt
      541 gttttgccgt ggaaacggtg acttgtaaac gcgcggaagc gtttcaatcc gcctttacca
      601 gtacgtttga aacgttttgc tgatccgcgg tgtgtttttt gttttggcat gactatattt
      661 cctcctcaaa atctttcgtt atccgactat cggctgtcca acagccagtt agtcattcaa
      721 attacttact gtcgtttttc ggtgccagcg tcaagaacat gctgcgtccg tccattttcg
      781 ctttttgttc cactgttgca atatccgcag tttcttcagc taagcgattc aagacttttt
      841 gaccaatttc tttatgggta atggcacggc ctttgaaacg gatagaagct ttcactttat
      901 cgcccttttc caagaactta cgtgcattac gaagttttgt attgaagtca ttcaagtcga
      961 tcgtaggact taagcgaact tcttttacat tgatcacttt ttgtttttta cgcgcttcgc
     1021 ggtctttctt ttgttgttcg aaacggtatt taccgtagtc catgattcgc gcaactggtg
     1081 gtttcgcacc aggggcaact aacacaagat ccaagtttgc ttgttctgca atattcaatg
     1141 cttctgcttt tgtcttaact cctaattgct caccatctga accgatcaat cgtaactctc
     1201 gtgcacgaat gccgtcgtta accatcatat cctttgctat ggtcattcac ctccaaatat
     1261 tatgagagaa agaaaagctt accgaataaa cggcaataaa aaaacgagtt ctcttacaga
     1321 acccgcgcgt acttcaccta atagaaccag tgaaaactat ctagcccagt gacattatca
     1381 actaaggcga gaagcgggtg cttctgcttt tatttctcaa ccattatagt atagcaaaaa
     1441 ctaatctttt tgtcaatgtt tttcatacag tttgtcttcc ttttctgaaa atgattagat
     1501 tagcaggctt tctagcagtg attcccgctt ctttgctata ctaaagaaaa acgaggtgaa
     1561 cccacatgaa aaaaatcgaa cctattccac cttctcttcc tcttcttaca aagggagagt
     1621 tttatttaga agatgaggag atcttctctg gtcttctgat caacggaggt aaccaaagct
     1681 atctcgatgc caaaaatatg gtattaagag agagtcatat ccaaaaactt acgatgcaaa
     1741 aaacaggttt acgtcatttt gaatgcagta atgtcatttt tgaaaaatgt gatttttcca
     1801 atctggagtg gattggtggc agtttccacc aagtcgtgtt tcatcaatgt aaattaacag
     1861 gtacgaattt tgccgaaagt tatcttcgag actgtacttt cactgattgt attgcttcga
     1921 tggcttcctt tagtagtaca aacttgaaag ctgtctcctt cgatcattgt caattagaag
     1981 acagcgaatt ttatgaggtc acttggaaaa accttttttt gtctgacaat cagttaaccg
     2041 gttcgaactg gtttcgaact tctctgaagg gattagactt tacgactaat ttcttcacaa
     2101 caatcgcttt atctcaagat tctttggcgg gactaatcgt caatcaagag caagcacttg
     2161 tgatcgccca agcccttggt ttgcagatca aagactagat caacgaatcc gccgccacag
     2221 tgcaataagc atccaaatag ataagaccaa actgatgatc aaggcgattt cccatccgaa
     2281 aacggaatgg gtaaagggta actggagatt cattccgaag aatcctgtca caatagttgg
     2341 aacggtcaag atcagcgacc agaccgtcaa aaatttcatc gtgtcattca agttgttatt
     2401 cagcaaatta ttataagtgc cagagagctg gtctaaaatc tgcgaagcta agttcgtcat
     2461 ttcaactgct tgtcgtgctt cgatcagtgc atcgtccaac tcttcttttt ccttttctga
     2521 caacttgcga taaatagcca gtgccttgat ttgttctaaa agtactacat tttgttttgt
     2581 tcctgtaact aagtaaacca aaccgatctc taaatctgaa agttgcagca gattcttgtt
     2641 cgttgttttt tctcttaatt tcagattaag ccgctgacgt tcactattca cttcctcgat
     2701 taaaggaaaa aaggaatcag aaatcaaaaa caatgtacga aacaataaac tgtatacact
     2761 catgtctggc atccgttcca aaagcgatcg aattagtcga atcacgtact cggtattcgg
     2821 cgtggtaaat gtaaacaaac cgtcttcttt taaaataaaa gcgataggac tagtttcata
     2881 atgattatcg actttttgct ggtgcggaac atttgataca agcagtaacg cttcatcaaa
     2941 agaatcgtac tctacgcgcg cccgttcatt tttatctaga gaataagtca gcatctcgtc
     3001 actaatgcca tattcttctt ttaagttttt aacagctagc acatcgtttt gctgtacatt
     3061 gatccaaaaa ttacggtctt gattaaaata gtgtttttcc cgcatactct atcctcctga
     3121 catttgtttc cagtataaag aaaaaagtcg ctcaaataaa ggtttaccct ttatttgagc
     3181 aactgtaaat caaatggata ctttggtttg gcgaatctct gctaattttt cttcgatcat
     3241 tttcaagact tcttcttttg cttgaggatc ttccacaaaa ttatagcggt ctccatcgat
     3301 ttggattttt ggactttctt tgtattcttc ataccattga tcataacgtc tgttcaattc
     3361 tttgtaataa tcgtatagac ttgggtcaaa ggacaattgt tcatacgaac gaccacgttt
     3421 ttcgatacgc tctaacatcg tatcaaaaga tacacgaata tgaactaata aatccggatg
     3481 ttttttatgg gcagcatagg gaagttcttc catcatgttt tccagcagtt catcatacac
     3541 acaaacttct gtttctgttg ctcttcctag atctgcattt agatggaaaa gcagtgaatc
     3601 ttcataaatc gaacggtcca atacattatt gtcatctttc attgcttgtt tgatgcttgc
     3661 aaaacgttta ttcaaaaaat aaatttgcag caaaaaagca tactgttcag ggtttgcata
     3721 aaataagggc aagacttcat tatcatcaat cgattcataa aatgcctgac tatcaaagtg
     3781 ttcagccatc atttctgtta aacttgattt tccggcacca attgtacctg ctaaaacgat
     3841 cacgctcatt tacctccagc tcacttcatc actaaatatt gtgtttaact ccaacctttt
     3901 ttaaatatac ccccaatatc tagtagtgtc aattgtattt tttctagtca aagaagaaaa
     3961 aaagtcaaaa aattcttaag aaaaaagtgt tttttcttta gaaaacgtat tcttagtttt
     4021 acttctgaga tggttttagt aaaataaagg aggaatgttt tttgagaccc ttagtggaca
     4081 aaaaaattcg tgaagcagcg caactcgttt tgggcgcttt attttttgct taaaaatcac
     4141 ttttacgtga gaaggagtta gattatggca aactatggcc aaggagaatc aagcggaaag
     4201 atcatattga tgggcgagca cgcggttgtt tatggagaac cagcgattgc ctttcctttc
     4261 tatgcaacaa aagtcaccgc attccttgaa gagctggatg caatggacga tcaactggtt
     4321 tcttcctact attcaggaaa tttagccgaa gctcctcatg cattaaaaaa tatcaaaaaa
     4381 ttattcattc acttaaaaaa acagcatgac atccaaaaaa acttgcaact gaccattgaa
     4441 agcacgattc ctgctgaacg tggaatggga tcaagcgctg cagtcgccac agcagtcact
     4501 cgtgcttttt atgattactt agcatttcct ttgtctcgtg aaatactatt agaaaatgtc
     4561 cagctttcgg aaaaaatcgc ccacggtaat cctagtggaa tcgatgcagc cgctactagc
     4621 agcttgcagc cgatttattt tacaaaaggg catccttttg actacttttc tttgaacatc
     4681 gatgcttttt tgattgtcgc tgatacagga atcaaaggac aaacaagaga agccgtcaaa
     4741 gatgtcgctc acctctttga aacccagcct catgaaactg gacaaatgat ccaaaaatta
     4801 ggatacttga cgaagcaagc aaaacaagca atcattgaga attcaccaga aacgttggca
     4861 cagacaatgg atgaatcaca gtcacttctg gaaaagctga caatcagcaa tgattttctt
     4921 aatctattga tccaaacagc aaaagatacc ggggcattgg gtgccaaatt aactggcggt
     4981 ggacgcggtg gctgtatgat tgcattagca caaacaaaaa caaaagccca agaaatcagt
     5041 caagcacttg aagatgcggg tgctgctgaa acttggatac aaggattagg agtacatacc
     5101 tatgtttaaa ggcaaagcac gcgcatatac caacattgct ctaatcaaat attggggtaa
     5161 gaaaaatgaa gaactcatcc ttccaatgaa caacagcctt tcgttaacat tggatgcatt
     5221 ttatacagag acagaagtca tcttttctga tagctatatg gtagatgaat tttatctaga
     5281 tggcacctta caagacgaaa aagcaacaaa aaaagtcagt cagtttcttg acctttttcg
     5341 taaagaagcc ggcctctcac taaaagcttc agtaatcagt cagaatttcg ttcctaccgc
     5401 agctggatta gcctcctctg ccagcgggct agctgcttta gcaggagctt gcaatactgc
     5461 tcttaagctt ggattagacg atctctctct ttcaagattt gctcgacgcg ggtctggttc
     5521 agcttgccga agtattttcg gtggtttcgt tgaatgggaa aaaggccatg acgacttaag
     5581 ttcttacgct aagccagtcc cttccgattc tttcgaagac gatttagcaa tggttttcgt
     5641 tttgatcaac gaccagaaaa aagaagtgtc cagcagaaat gggatgcgtc gaacagtcga
     5701 aacatccagt ttttatcaag gctggttaga ttccgttgaa ggggatctat atcaattgaa
     5761 acaagcaatc aaaacaaaag atttccaact tctcggagaa acgatggaaa gaaacggact
     5821 aaaaatgcat ggaacgactt tagctgccca gcctccattc acttactggt ctcctgacag
     5881 cttaaaagcg atggatgcgg tgcggcaatt gagaaaaaaa ggcattccat gctactttac
     5941 gatggatgct ggccctaatg tcaaagtttt agtggaaaac agccatttat ctgaggtaca
     6001 agaaacattt accaaattat ttagcaaaga acaagtaatt actgcccatg ctggtccagg
     6061 aattgcgatt attgaataaa gggagatttt tatgattgaa gtatctgcac caggcaaact
     6121 ctatattgcc ggagaatatg cagttgttga aacaggacat ccagcagtta tcgctgcagt
     6181 cgatcaattc gtaacagtca ctgtagaatc cgcacgaaaa gtcggaagta tccaatctgc
     6241 acaatatagt gggatgcctg tacgttggac aagacgcaac ggagaattag ttttagatat
     6301 tcgggaaaat ccttttcatt atatccttgc tgctattcgt ttgactgaaa agtatgcaca
     6361 agaaaaaaac attcttttat ccttttatga tctgaaagta acaagtgagt tagacagttc
     6421 aaatggccgg aaatatggat tgggttctag cggtgccgtg accgttgcaa cggtcaaagc
     6481 cttgaatgtt ttttacgcgt tgaatttatc tcaattggag attttcaaga ttgcagcact
     6541 agccaattta gcagttcaag ataatggttc ctgcggcgac atcgctgcta gctgttatgg
     6601 tggctggatc gctttctcaa ccttcgacca tccttggctc caagaacaag aaactcagca
     6661 ttctatcagt gagttacttg ccctggattg gccaggtcta tccattgagc cattgattgc
     6721 tcctgaagat ttacgtttat tgattggttg gacgggtagc cctgcctcta cttctgattt
     6781 ggtcgatcaa gttcaccgtt cgagagaaga caaaatggtg gcttatcagc ttttcttaaa
     6841 aaacagtaca gaatgtgtca acgaaatgat caaagggttt aaagaaaata atgtaacgtt
     6901 gatccaacag atgattcgaa aaaaccgaca attactgcat gatttatctg caatcactgg
     6961 ggtcgtcatc gaaacgcctg ctttgaacaa attgtgtaat ttagctgaac agtatgaagg
     7021 agccgcaaaa tcttctggtg caggtggtgg cgattgcgga atcgtaattg ttgaccagaa
     7081 atctggcatc cttcctttaa tgagtgcatg ggaaaaagca gaaatcactc cactgccgtt
     7141 acatgtctat agcgatcaaa gaaaggaaaa ccgatgaatc gaaaagacga acatgtttca
     7201 ctagctaaag cctttcatga taaacaaaaa aacgaatttg acttcgtacg gatcatccat
     7261 aaccctttgc cgcaaatagc agtttctgat gtcgatctaa gtacacaggc agttgggttt
     7321 acactaagca gcccatttta tatcaatgcg atgactggcg gaagcgaaaa aacaaaaaaa
     7381 atcaaccaag atcttgcaat cgtcgcaaga gaagcagatt tgatgattgc cactggttca
     7441 gtcagcgctg ccttgaaaga cccttcttta gccgatactt atacgattat gcgacaagaa
     7501 tatcctcacg ggaaaatcat tgccaacatc ggtgcaggga cttctgtcga aagagcccaa
     7561 gaagctatcc gattgtttca cgcagatgcc ttacagattc atttgaatgc acctcaagag
     7621 ttggttatgc ctgagggaga ccgtgatttt accaattgga aagtacttat tcaagagact
     7681 caaacagcca ttgacgtccc cctcatcgta aaagaagtcg gttttggcat gacaagagaa
     7741 acgctcaatg atctagctgc tttaggagtt catacagttg atatcagcgg tcgaagcgga
     7801 acaagcttca cccagatcga aaacgcccgt cgctccaagc gggaactgag ttatttagct
     7861 gactgggggc aatcgacagt ctcttcattg cttgaagcaa atgaagcaga cacttctatg
     7921 gaaatcctag cctccggagg tatccgcaat gcctatgaca tatttaaagc cctttgtctc
     7981 ggcgcaaatg cagtgggtac ttcaggaacc gtactgacgc atctgatgaa tcacggcgta
     8041 gaagaaacta ttatactgat gaagcaatgg caagaagagc ttcgtctgct ttatactatg
     8101 gtcggcgcaa cgaataccgc ggccctacac caacaatcac tgatcttttc cggaccagtg
     8161 aaggattggt gcgaagcaag agggatcgac ttagtgaagt atggaagacg cacagaaaag
     8221 agcgtgggac aaaagtaaag aacactttta tcccacgctc taaacacgta taaacggcgg
     8281 aagcaaaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     8341 cgtgaattcc ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttcgaat
     8401 aaatgatgtt taatcagctg tccttcggta ttcgttcaag ccgctgattt cacaacctct
     8461 ccactaaacg gtgtactaaa gctcttttaa ggttgttttc cgatataggc aagtatgcct
     8521 ccgtccacat acaagacatg gccgttaacg aaatcagatg cgctagaagc caagaagaca
     8581 gatggtccag ccaaatcgat tggctcgccc cagcgtgctg ctggcgtacg tccaacgata
     8641 aattgatcga atggatgacg ttcaccgttt tcttgtgttt cgcgtaatgg agcagtttgc
     8701 ggtgtagcaa tataacctgg tccaattcca ttacattgga tattgtattg accgtattca
     8761 gaagcgatgt ttttcgtcag catttttaat ccgccttttg ccgcagcata agcactgacc
     8821 gtttcgcgtc ctaattcact catcatggag cagatattga tgattttacc gccgcctttt
     8881 tcgatcatat cccgaataac tgcttttgaa acgatgaatg gggcatttaa atccacgtcg
     8941 attacttggc ggaagtctgc cgcatccatt tcgatcatcg gtgtccgttt gatgatccct
     9001 gcattgttga ctagaatatc aactgaacct acatcttctt tgatctgttg aaccatcgct
     9061 tgaaccgctt cttcatcggt cacatcgcag acataacctt ttgcatcgat gcctgcttct
     9121 ttataatgcg ctataccttc atcaactgat tcttgattca agttattaaa tacgattttt
     9181 gctcctgctg tagctaaaga gcgtgcaatc tcaaatccaa taccatagac agcaccggtg
     9241 actaatgcta cttttccatc taaacgaaac atatccatac taaattccat cattttctct
     9301 cccttatgct tattttaatt gatccatttg caccatatcc atgtctgtat aggtgatatt
     9361 ttctccgcac attgcccaga tgaatgagta attgcttgta cctacacctg aatggatcga
     9421 ccagcttggc gaaataatgg cttgttcatt gtctacgact aaatgttttg tttcatctgg
     9481 tttgcccatc atatggaaca cacgtgtatc ttcattggca taatcaaaat agacataagc
     9541 ttccatacga cgttcatgtg tatgacaagg catcgtattc caagcacttc ctggttctaa
     9601 aatcgtataa cccatttata aatgacagct atcgctgaag cttctactgc tgtaaatatc
     9661 cccgtcaaga taccaccgat aatgatgaca atcaatagaa ggctaggaat cgcctctttt
     9721 attatttttc cagcatgagc catctgtcct ttttcgacaa cagggtattt attcttttta
     9781 gcaacgatga atgctactag catgattcct agcgcccaca aa
//
LOCUS       DAZUMV010000105         9665 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000105 DAZUMV010000000
VERSION     DAZUMV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.106"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..632)
                     /locus_tag="ACG8FK_002194"
     CDS             complement(<1..632)
                     /locus_tag="ACG8FK_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5097763.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="ACG8FK_002195"
     CDS             975..1391
                     /locus_tag="ACG8FK_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5097764.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="ACG8FK_002196"
     CDS             1388..1858
                     /locus_tag="ACG8FK_002196"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5097765.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="ACG8FK_002197"
     CDS             1872..2645
                     /locus_tag="ACG8FK_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5097766.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="ACG8FK_002198"
     CDS             2659..3471
                     /locus_tag="ACG8FK_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5097767.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="ACG8FK_002199"
     CDS             3525..4490
                     /locus_tag="ACG8FK_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5097768.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="ACG8FK_002200"
     CDS             4606..7329
                     /locus_tag="ACG8FK_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5097769.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="ACG8FK_002201"
     CDS             7342..7974
                     /locus_tag="ACG8FK_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5097770.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9665
                     /locus_tag="ACG8FK_002202"
     CDS             8873..>9665
                     /locus_tag="ACG8FK_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5097771.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3260 a         1477 c         1884 g         3044 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtatt
//
LOCUS       DAZUMV010000106         9642 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000106 DAZUMV010000000
VERSION     DAZUMV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.107"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..1279
                     /locus_tag="ACG8FK_002203"
     CDS             125..1279
                     /locus_tag="ACG8FK_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFS5097772.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1252..1695
                     /locus_tag="ACG8FK_002204"
     CDS             1252..1695
                     /locus_tag="ACG8FK_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097773.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            1997..2377
                     /locus_tag="ACG8FK_002205"
     CDS             1997..2377
                     /locus_tag="ACG8FK_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS5097774.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            2359..3273
                     /locus_tag="ACG8FK_002206"
     CDS             2359..3273
                     /locus_tag="ACG8FK_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5097775.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3233..3511
                     /locus_tag="ACG8FK_002207"
     CDS             3233..3511
                     /locus_tag="ACG8FK_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097776.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDL
                     QVKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
     gene            3513..4202
                     /locus_tag="ACG8FK_002208"
     CDS             3513..4202
                     /locus_tag="ACG8FK_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097777.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4322..4408)
                     /locus_tag="ACG8FK_002209"
     CDS             complement(4322..4408)
                     /locus_tag="ACG8FK_002209"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5097778.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            5054..5986
                     /locus_tag="ACG8FK_002210"
     CDS             5054..5986
                     /locus_tag="ACG8FK_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5097779.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            6003..6473
                     /locus_tag="ACG8FK_002211"
     CDS             6003..6473
                     /locus_tag="ACG8FK_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5097780.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            6557..7009
                     /locus_tag="ACG8FK_002212"
     CDS             6557..7009
                     /locus_tag="ACG8FK_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5097781.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFS
                     HSIATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
     gene            7136..8317
                     /locus_tag="ACG8FK_002213"
     CDS             7136..8317
                     /locus_tag="ACG8FK_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5097782.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            8407..8676
                     /locus_tag="ACG8FK_002214"
     CDS             8407..8676
                     /locus_tag="ACG8FK_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="HFS5097783.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYK
                     KYPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWNASSRTAKYYYYI"
BASE COUNT         3567 a         1529 c         1589 g         2957 t
ORIGIN      
        1 aactaacgct ataaataacg attatgttcc caaaacaata ttttatgaac tataacaaaa
       61 atttgttatc gcatattgta tattttaatt ataggtgtat accacttaaa tttaacacaa
      121 aggcgtgatt attttgaaac ccaaaaagaa aatttatcta ggtctcctct tttccttgat
      181 gattggaatt tttagtatag gaattacaaa tacctatgct tcagaaaatt ctaatttcaa
      241 tatcaactcc caaccaattg aagaacaagc aattaatttt gcgaaaactg attttgaaca
      301 ctccataact tcaattcttt taatggaaaa taacaccgaa actgatatta actatactct
      361 tggttcgcca tttaaaatgc tcgatggaga aaatacggca aatagttcta ttttctttcc
      421 agtattttca gacgctgata atacgattaa atatatttat accgtcaaaa aaaatacaaa
      481 tggagaatat tcaggaagta tttcacaatt tttagcaaaa gaacttcaaa atctagtaga
      541 tagtaatata acagatacaa ccttattttt tcaagataat agcatttatt attccgataa
      601 tggacaaatt aaactacttt ggaaatcacc tttaagtaca gaaaaaaatg gtgtttcttc
      661 ctttacagaa aaaaatgatc ttatatccgt aaatataaaa aataaaatta aaaatttctc
      721 taaaaaacaa aattttcctg cgattaaacg tgtaaaaagt gtctcctccg gagataactt
      781 tattatgatt aattggcgta tagcagaaac ccaaacaagt tttccgtggt gtgctgctta
      841 cgtagaagct gctatcttat gtaacaaaac agatgtacat gctacttcag ctagagccat
      901 tatgcattat acctatccta atttaaacaa cagtcaaatt gaatcaaaaa gtcttaatca
      961 agatcaaata ataaaatatg caaattactg tggtgtttat cctaaaaaag tagcacgaat
     1021 actaaacctt tcagaggtcc aaacacaact aagaaaagga aatgccattt atatgggaac
     1081 tgctggttat ggaaattata gtaacagccg tcatgcactt gcattatttg ggtgggtcaa
     1141 tcaaggtggt tatcaaactt actatgtgtg gaatccttgg tataactacc caactgtaat
     1201 aagtgcaaat gcaggaccag ttacaattcc tgtacctgga ggtggtttta cttgggacaa
     1261 taccattaca aattggtaaa taaacccatt gctttagtta tgggtctagt gttagttctt
     1321 ttcttaacta gttgttcaaa aaaacaagaa atacaaaaga ctgatctgat tccttttcta
     1381 ggaacttggc aaactaaaga tcattcagaa cgtctagtaa tcactaattt aaatacttca
     1441 aaaaatatag aacaatatac catcttagta aaagagaaca atcataaaat tcaaatcatt
     1501 gataaaaatt taaatataaa tgaacaagtt ttcatttaca aagcaacaaa taatttacta
     1561 acactgcgct ctaataaaaa acaattagat tacactttta gtttggattc tccaaaaaag
     1621 ctcgtattta ttttgacaac gaatgatggg acagaaggga cagctaaacc aattgaattt
     1681 tttaaagctg attgatgaat agttgtttga tattacaaaa caataatttc accgaaggtg
     1741 aaacaagcga aactttacaa aaagtggcta gcatttgcta gccttttttg tattgtggag
     1801 cgtgggtttt gagggggatt caagggggag cttgcgcccc tttgaaaagc ggttatttgc
     1861 accaaatacg taggtatttg gctagcaaat atagagcttg ccaaaccctt ttagtgagct
     1921 ttactcattg taccttttta ggtacaatgg tattgcgaac gatacgtccg taggacgtac
     1981 tgaaagggtg ggacttatga gcgaacaaaa tcaaaatttg gctagcgatc attctaaaaa
     2041 atatacctat cgatccgagc caaaacaaat cagttttcga gtgagcgaat ctgaatttgc
     2101 aaagctgaag cagtcagctg aagctttgca aatgagtgtg ccggctttcg taaaagccaa
     2161 ggcacaaaac gcgcgtctgg tgacaccgaa agtggcgcca gacattgccc aggcaatcgc
     2221 gcgcgattta gccaaagctg gagggaatat caatcaaatt gctaagtggt gtaatacaca
     2281 ccaacacgac gttgctcctg gagacgccca acgcttatcc gaaaacctaa aaatcatgca
     2341 aaaggagcta caaaagatat ggcaacagtt aaagtaagcc gaaccacttc ttgtagtcgt
     2401 gcaattaatt acgctgaacc acgagcaacc attaaaacag gaataaactg tgacgtcgat
     2461 tatgccaaaa gtgaaatgaa acaaattcgc atgctgtatg gaaaagacga ccgtgtacag
     2521 gcacacttat tgattcaatc ttttcgacca ggagaaatta ccgctgaaaa agctaatcaa
     2581 ctaggggcag agtatgcaaa aaaaatagcc cccgatcacc aaatagctat ttacacacat
     2641 acagacaaag accatattca taatcacatt gtcattaact ccgttaatct ggagactgga
     2701 aaaaaatttc aagctcatgg tcaagcattt ctaaacaaat gttatgacct aaatgacgag
     2761 atttgtcttg cacataactt accaatcact gagcgaggaa agaaaccaga aaaacgaacc
     2821 atgtctgaaa taaaattaaa agaaaaaaat gaacctgttt ggaaagacga aattcggatt
     2881 gcgatcgatc aaacaatgaa aaacaaaaaa accagaacct atgaccaatt ttgtaattcc
     2941 ctcaaaacat tggggattca ttgcttcaat cgcgggaaaa atttcactta cgaattgatc
     3001 cacaaaaaga aaaaagtacg atccaacaga ctaggaaagg actacgaaaa ggagacgatt
     3061 ttacatgagc tggacagacg agaacgaacc tataaccaac agcgaaccaa cgagctacga
     3121 agagctcgag ctagcattcg cgaaactgca gaagaagtac gaagagaaca acgaactctt
     3181 acgcctgaac aaccggaaat tagaccggct cgaagaaacc atgaacaaca cgttgaaaga
     3241 ggaacagacc accaacaaga attatcgctc taaaacgtta aaagacatag aaaacgaaat
     3301 aaagaaacaa cgtgcttttc tcgttcctta tacgcaagaa aggatacaaa ctacgattga
     3361 tttacaagtt aaaaaactcc atcaagagct attagaccag cgaaataagg aacagaaaaa
     3421 ggtgcttatt caacgatcat tggattttct tgccccctta tgttgcttag tcctgacact
     3481 gttcgcattt tggttagtat ggggaggcta aaatggcatt ttatttaccc tatctactca
     3541 tttttgttag tatttcagga agtatttggc tcatctataa aatttttcaa acaagatatt
     3601 cattaaaagg ctctaaaatt cgatttaagc gatttttctt tcttggctgt atattttcct
     3661 tgataatcgt ttcaagcggc ttattcggcg ttttagaggg aaataagcgc gtttctagaa
     3721 gtattttatt aggaaatgtc actcaaaagt atgaatcagc gcgtgataaa aagaaaaaag
     3781 agcaagtatt agcacaaaaa atagaaaaat ttaccgcttg ttatgaggac atgaatgata
     3841 tttttgtgaa gcaagaaaag cgtttgacgg ataaaaatat ggaaacactc actcgacttt
     3901 atcggaattt gccagaagaa cagcaaaaag agtaccaaga aaaatatgaa caagtaaaaa
     3961 aagacgtgca atatgtgaaa gatacgaaag tggaagaagc ttgtagtgat ttatttggag
     4021 atacaattcc tttttcaact agcgaacaag aacgaaaaga acgacaacaa tcagtcacat
     4081 atgaacgcta tgaaatcttg cttcaacaag caattaacat acaaaatctg actaaaaaag
     4141 aaacagcttt aaattattta aaaagcgtca aggaatggtt agatcagcaa cagcaaaatt
     4201 gaatttgatt gaaattcatg ttaaactaaa catgcaaatg ttcaaacatt agatacacga
     4261 aagccccaga gaggtcggat tctctggggc tttttttagg ttgatgtcgc ctttactgat
     4321 tttacttctt gtttcggtta ttcaaccaat aactgaatac agagatacaa actcctacaa
     4381 gaagggggca aataaacagt tcaaacatca gatacacctc cttccagtcg caagactgtc
     4441 ggataggcga caagttatta taccctacaa aattaaatct gtgtggcaat tttaagtatc
     4501 tcttatttcc gggttgttag ggcttgccct gaccgcctgt agggcgcttt aaatgccaga
     4561 taaaggtttt tatactaaga ctgtcttaaa acggcttaga caagctctta gagcctcaac
     4621 cctccttttt gaacaatggt tttagcaaag gagctggagg cgactgcgca ggtataatgt
     4681 gagagcgtag cgatcacacc tttttggcga ccgtagggag cgaggttttg aattagcagt
     4741 ttagaaaagt tcgcgaacct gtttaaagaa aaaataattg tttttttagg tgggaaattt
     4801 tgtctccctc ttttataatc tttttttaaa cctcttttta aacctcttta ccgtcggctg
     4861 tgtccttact cccccaaggg atacaaaatt ttataggcat gaaattatct gtttataggc
     4921 atgaaattat ctgtttatag gcatgaaatt atctgtttat aggcatgaaa ttatctgttt
     4981 taggtattgt ttttagacat aatatgtcct aaaatgatgt caataaaatc atttagactg
     5041 gagtaacaac taaatggcaa atgaaatcgt aaagtatgaa ccagaattaa ataccattcc
     5101 tttaagaaaa tttaccccaa tagaaatgaa cttgtttttt tctattgtct ctagaatgcg
     5161 cgataaaggt aatcaaaaaa tacgctttta tttcagccaa cttaaagatt taagtgcgta
     5221 taagcagact gcaggagaag tctttataaa agatttggca agaacatacg atcatttgat
     5281 ggatttacgt ttcggaagaa aaagcaatgg tggattaaca atagaacgat ttacaatgtt
     5341 tactgatttt gaaattcatg gagaagtcga taacccttat gtagatataa aagtccacga
     5401 aaaagctttg cctttactta ataatctaga aagctgggta cgtttctctt taacagaatt
     5461 tcgagattta aatagcagtt atgctaaaac aatgtttaga ttgctaaaac aataccgaac
     5521 tcaaggatgg gctgaatttt ctaaggaaga ttttttagag ctattagata ttcctaaaag
     5581 ctatagacaa acaaatataa atcagtttgt actcaaacca ataaaagaag aactaactcc
     5641 actatttaaa ggattaacta ttcgtaaaaa atatggtaaa ggtcgaggta aacctgttat
     5701 tggttatcgt tttacttgga aagctgaaat caaccatgct gacgattttt caaaagggaa
     5761 gcaagaagat ttacgtataa aattatttaa tattgaacac aatggagaat taacacaaaa
     5821 agaaaaatgg cgtgctaaag atcgaatact aaatcttccg ttaggtactc atgaagctga
     5881 ttttaaaaaa caacaacaaa ccgaaaaaga agaagcggaa aaacaatcaa tctcaaatga
     5941 gcttaaacaa gatttattag aaaacttaca aaacttattc gattgaagca taaggagtat
     6001 ttatgataga taaaacgatc aaagaactcg cggaagaatt tcatgtttca aaacaagcaa
     6061 ttaggaaaag gctatcagct gattttagag aaaactatgt gcaaactgtt actaggaacg
     6121 gagtgaaaac cttagttgtc agctacaaag gatatttgct tttaaaacaa cattttttag
     6181 gtagtaacac cactggtaac caagtagaaa ccgttgctag taacgatgaa aactcgatta
     6241 ttgttttttt acatgaacag ctccaagaaa aagatactca aataaaacaa atgcaaaagt
     6301 tactcgacca gcaacaacaa ctcaccttgc aggcaaatca acagatcgaa aaactgcaag
     6361 aacagttaca attagcttat acggaagaac ctgaagaaga ggaattaact tctgtcaata
     6421 aagaaaacaa caatgcaata acaacagaaa aaaagtggtg gaaattttgg taacaattac
     6481 caactttcca taaagaagaa atcaatttct atagactcta tctttctaag aataaaaaat
     6541 atgtttaggt gatcaaatga ctataagtaa agtaatcaaa caaaaaagaa tagaaaaaca
     6601 actaactcaa gaagatttag ctgaaatgct tttagtatct aaaaaaacca tttctaattg
     6661 ggaaaatggt cgaacaatac cagatacaga aaatttaatt cagtttgcta agttatttga
     6721 tttatcacta gacaatattt tgttaggaga agaaaaaatt atggtaaaaa atcatacaaa
     6781 aacactaata ctattatttc attttgtaat ttttttaagt ttagttttca ccttaattca
     6841 gtccggattc ttttatttta gccattcaat tgccacttgg ttattaatat tttttctaag
     6901 tttactatta ataattaata ctttaattat tatctttaat aaacatagaa acttatttct
     6961 tcacagcata tttccaattg ttttattaat ttttctatac tttatataaa aagatagaac
     7021 tatttagtaa gataaaaaag tagctaattg taattttcgt ctaaaactca aaactagtaa
     7081 aaataacttt attgtgttat ttttatttat aaattatgaa aaaggacctt tcgaaatgaa
     7141 aaataataga atctacaaaa atcagttgaa ttatgggctt ttattgccag tttttctgct
     7201 atgtatcatt ggattacttt ctttatatgt tgctttatat cacgactctt acactaattc
     7261 aaaaatcaca aaagaattaa ttaaacaagt attgtggtat agtattgggt tcacttcagc
     7321 aataactatt tattttttta atcctaaaat tttgtggaaa ttaactccta tattttacgg
     7381 attatgcatc ataatgttat tacttctctt gaaattctat aatccacaat tagcagtctt
     7441 aactggagca aaaaattggt tttatattgc tggtttttcg tttcaaccta ctgagttagt
     7501 caaaatagga tacaccctta tgctagctaa aataaccgtc aattatcagc aaaaatctat
     7561 tactagtaat acgaaagatg attggattta tttacttaaa acttttattg ctactcttcc
     7621 aataggaata ttattatttt ttcagaacga tctaggaaca atgttagtct tcattgttat
     7681 ttatattttt attttgttta cgtctaacat aaatataaaa ataattgccc caataatcgc
     7741 cattggtgtt gttttatcct taatattttt atatttggtt atatcagata caggaagaga
     7801 tttcttatta aaaataggat ttcacgacta tcagtttaaa agaatagact catggttgaa
     7861 tccattttta gatccaaatg gatctagcta tcagttagca aatagtttaa ttgctattgg
     7921 ctctggcgga cttttaggta ctggttttaa tgtatcaaac attcacgttc ctgttagaga
     7981 atccgatatg atattttctg taattggaga aaattttggc tttataggaa gttgtcttgt
     8041 aatattccta tacttttatt taatttatca aatgtataaa acatgtttta aaagcaataa
     8101 cttatttttc acctatatcg gtataggcat tgttgcaatg attttttttc atgtttttga
     8161 aaatataggt gcaagtatcg gattactacc tctaactggt attccactcc cctatattag
     8221 tcaaggtggt tctgccattc ttagtaattt tttggggttg ggatttatgt tgtgttgtag
     8281 tagatatact aacgaaaaaa aaggaacaca aaaatgaatt caaaataaca ctttatgaat
     8341 agaatgtcta tttcaataaa aattatgata ccttaaagtt aatcattaaa aaaggagtgt
     8401 cataatttga aaaaaacaaa attattggta gctagtttgt gtttgtttag cagtttatta
     8461 gctttcaccc cttctgtttc attttctcaa aatggtggag tggttgaagc tgctgctcaa
     8521 agaggatata tctacaaaaa atatccaaaa ggtgctaaag tgccaaataa agttaaaatg
     8581 ttagtaaata ttagaggtaa acaaactatg cgtacttgtt accttatgag ttggaatgct
     8641 agtagtagaa cagccaaata ttattactac atttagtaaa aacaaaacat tctttctata
     8701 caataaaata gcgtatagag aggatgtttt taatttgaaa aggagtggtt atctttatga
     8761 aaaatagatc atttgctata ttagtattat ccgtaatttt attagatata ttgttaataa
     8821 cattagcttt aaaaacttta aatttagtaa caagttttgt cattatacca gtaattatag
     8881 gaatgtctat ttatttcatc aaaaaaaata aacaattttt ttgaatgaaa ttacgggcga
     8941 aaataatcac tcaaaattat taataaaaac gaatcaacta ctttttctga agaaacatac
     9001 aaccgtaaac aacaattgtt gtttacggtt gtaaattatc aaactatatg gtgacttgtt
     9061 gcttaaaata ggttgaaatt tcgtttattt tttttgagaa attttaccca cgtactaccc
     9121 atgtacctat ccacgtatta cccatgtacc cccccatgac aaatgggcgg aggggcttgc
     9181 ggcgcttggt attaaaggca ttatccgcct cccaggtttc tgaccatttt ctctcataag
     9241 tggggttttt accgcgttga gagaaaatgg tcagaaacct aaacgatcgg caaagaaaac
     9301 ataccaagcg ccacaagcaa aaaaagttag agtaacttcc agaaaagaaa aatttttaga
     9361 acaacttttg aaaatttgat tttgatttat atccgttcaa tttaaacatt tgataaactg
     9421 aaggctagta tttaaaatgt gtatacaagt ggtagcgtta aaaaaagtgg aggtataacc
     9481 tatgttaaaa aaaactgtgt tggtactggg ttctatttct ctaggaatat taggttttag
     9541 ccccatgcta ttatccgtaa ttggaaaaaa caattccaac agagaactta aattaaattc
     9601 aaattattac aacagtccta acgaagaatt gtttgtaaaa ca
//
LOCUS       DAZUMV010000107         9511 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000107 DAZUMV010000000
VERSION     DAZUMV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.108"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            771..1574
                     /locus_tag="ACG8FK_002215"
     CDS             771..1574
                     /locus_tag="ACG8FK_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5097784.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            2159..2614
                     /locus_tag="ACG8FK_002216"
     CDS             2159..2614
                     /locus_tag="ACG8FK_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097785.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2733..3791
                     /locus_tag="ACG8FK_002217"
     CDS             2733..3791
                     /locus_tag="ACG8FK_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFS5097786.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3833..5782
                     /locus_tag="ACG8FK_002218"
     CDS             3833..5782
                     /locus_tag="ACG8FK_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5097787.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5785..9426
                     /locus_tag="ACG8FK_002219"
     CDS             5785..9426
                     /locus_tag="ACG8FK_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFS5097788.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         3399 a         1374 c         1950 g         2788 t
ORIGIN      
        1 atatttttct agtttttaga aataaattca catacttttt ctttttttgg atatattcgg
       61 aataccacat cgctttttgt ccacgtttgc atcgcttttt gtccacattc acatcgcttt
      121 ttgtccatgt tcgcatcgct ttatgtccac cctaaaatct atttaagatt gatataaagc
      181 ctttttggag gttgctaaag ttttaaagat taaaatatat attataaata taaataaaag
      241 gggaagctga agcttcctcc ttttcctttt tttcaattat tcaaagttac acaagcaaaa
      301 taaactccta gatagtgtta gtttttttta agatttttta tttttaagat tttgaaataa
      361 gagaggagta atcctccttt attaaatatc tgttaatata tctgttaaat atctgttaat
      421 atatctgtta ggggaggctt aatctcttgg gagagtaagt attaaagggg tataaaggta
      481 acacttttgg acagtaaagg taacaaaagt ggacagtaag ggtaacagtt ttggacagca
      541 aaggtaacaa aagtggacat ttgaggtatc acactaggat aagacgttac cttcggtttt
      601 cttgatttgg aaggcgaaaa aatagaaaat tagattagta acaaatcttt gttgactgtt
      661 acctttagtt gtgatttaat ttaggtaaca agcagaaaaa attgtttcct ttaattagta
      721 ccagattaac atgattaaag gagcgcaaaa agaagattgg agagtaatca atgtctcaag
      781 aagttaccga aaagattatt gatagtgatg tagatttagt atcagaacaa acaaatgcaa
      841 ttgtgcgtta taacaatggt ctgaatacaa cagctttgcg aggatttaag cctgttgaaa
      901 tggatgtgtt ttgggctatt ttatcaaaaa tgaaacgtca aggaacagca gaagtaactt
      961 ttgatttcaa cctctttcgt tatttggtca aatacgacag aagagaaaag gacagttttt
     1021 attctgtgtt aaaagatttg agtgacaaac taggtaattt aacctaccgt tttgaagatt
     1081 cagacgtcta tgaacagctg tggctttttc aaaaattccg gatagataaa cagaaggaaa
     1141 cagttacaat taaggcaaat gaggaatttg aatttatctt aaactcaatt ggcaaaaact
     1201 ttactcgttt tgaacttgat aattttacaa aactaagaag tagctacaca aaagaattgt
     1261 atcgtcatct catgcaatac agaaataaag agtggcgaaa tggttattgg gtagtcaaag
     1321 ttgaagattt tagaagagca ttaagtatac cttcaagtta tagaatgaca aatattgata
     1381 aaagaatact agagcaggcg aaagaagaat ttttagctcc tgatgaaaac ggaatacagg
     1441 tatttgaaac ttttgattat gaaaaaatct atgcaaagaa aggaagaaga gttgatcggc
     1501 tcagattcac ttttagtgag ccagaatcaa acttgccaac catttcaatg cataattggt
     1561 tagaagaaga ttgagaagaa gcaggttgta attttaatac aacctgcttc tttaaaaaag
     1621 tatgggtaac acttttggac aataaatgca agaagttttt ttgtaagcaa gtttcagtct
     1681 aaataatatt tgaaagaatg gctaatctat acaaatttta acaactatgg gtaacacttt
     1741 tggacaagtc aagaaaaaaa acaatttttc ttaatttgtt gatcgactaa aggtaacact
     1801 tttggacaac acagaataaa aagtccggtg atatcagttt tggtaggaaa catcttaata
     1861 tctttgtgag attgaaggta acatttttgg acaagtcaac tatttgttaa aagttaatat
     1921 agagtaatta gataagtttt ttcttttcgt ctgataaatg gataatatta gttattttat
     1981 tgctaattag tctttcatga tgactgaaat agctaaaata aatatctcgt tggataattg
     2041 ctgatacttt aagggatatc taaatttgct gattgattta tttatgttat tatgatatta
     2101 taataacata aatgtgttac gcccgaacct cattttaaaa aaatgaaggg gtgagacaat
     2161 gaatgctatt aattggcatg aatttgttaa ctatatggag agcttactag atgatctaaa
     2221 aagttttagt attgctgatg cagtaaagca tgggttacca gttagtacaa cgatgaaatt
     2281 acgaggattg aaaattccag atgaaattat taagctgaga actaaaacaa ttattgaaac
     2341 cgcaaaagtc gttgacaata tgaaccatga actaaaaggc aagaaaacaa cttacgagaa
     2401 agattttgaa cgttttttaa aatctgttat tggaaaatat ctaggtaaaa aatctttcag
     2461 tgaattgaga gatgaagaga ttgtacctac tccaatggtt agcaaaatcc gaaaaaacaa
     2521 agccgagggg aattactatc ttggtacaat agcaaaaatc attgattatt tggaaaataa
     2581 agagcaaaag aaaggaacga agaaaggaag ataatttttg tgttaaatat taatggggaa
     2641 gaatataccg aaatgattca aaatcccgaa cctagaatta ccgaaaaaac gtctgattgt
     2701 ttgctctttt atgaaagggg gcagacaaac taatggaaaa aaacgtcaat tcaacatcta
     2761 ccaaagatca atacagtatt attggagggt tgcttggtga taaggataag agaacacgtc
     2821 acggattacg cattatgctt gcaacttttc taggagtgct ttttttatta acaggcttgg
     2881 gtagctacat ttatcaatta ggaaactata attattatct cgtgaacaat acaacaccta
     2941 taggctcttc attggagttt gctcgatcag gagcagagat cactatttca gatgtgtgga
     3001 cagatcaaaa cagagatgta acagtagtaa aactcggtta ctcgcaaaac gctagaaagt
     3061 tgttatctac gcaaggaaaa aactacaact tatatatggc aacgaaagaa aaagatcgtc
     3121 ctaaaaagct tgaaatgagc tatgggatgt taagtactga tggggatgct ttcttattcc
     3181 taaaaggtaa attagatgaa agagcttatc aagtttttat tgcaaaccaa gtatcactaa
     3241 tttctggtga tgatacagct gacgatgata ccaaagtcaa tgtgaatgaa gcagattcag
     3301 ttacaaaagc actttctcaa tattctatga atgatgttaa taatgaagga gtaatttctt
     3361 ttgggaaaaa agcaaaacct gttaatactc ctgataatat caatttcaga ataaatccat
     3421 attcagattc aactaatgtc tatcaaggaa gctttcttac caaagatggc gatattgatt
     3481 attcaaaagt tgtagcagtt acaagtaaga ataatgttgt aagtaagtta gaagatcagt
     3541 taaatgcttc aaatactaac atcaagaaaa ttgaagctac aaaagctgag tatctggaac
     3601 gtttacaaca aaatcctgac gataaagatt cgcaagaaag tattcgtcaa ctagacagtc
     3661 gaatggagac cgaaattaaa gcaatcaatg atacacaaaa attgattgat caatacaaaa
     3721 atgctgactt taacaaagat tcttttggag acatgaagac gacatttaag tacctaactc
     3781 tgactgacta gaagtaaatt attcttattt tagaggaagt gaacacaaac agatggaaaa
     3841 tagtaccgag ttttttaaga atattcaaac tgaatttaat catgctctca caccaatgaa
     3901 accaccgaaa cataagttag tgggacgtga tgacgaactc catgagttgt cactgatctt
     3961 gaatagaccg gaaacaccgc aagctatctt gttaggtgaa gccggaacag gtaaaacagc
     4021 acttgcaagt gaatgggctg aaagacagcg acaagatgga aagaaagtac aagtatacaa
     4081 attatcaatc ggacaattgc gtgttggggg agatgatgtc ctccaaacac gtttagaaac
     4141 attaattcca agattgaaag aatatgagcg aattttaaaa tctgaggaca aagaaaatga
     4201 atttgtcctc ttcattgacg agtttcataa agttgttagc tcttttggta gtggcaataa
     4261 aattgggggt gattcgctaa aagaaccgtt agcaaatgaa tcacttcaat tgattaccgc
     4321 tactacaaga aaagaatatg aaaactatat caaaagagat gatgccttag ggagacggtt
     4381 acaaccgtta gaattgaatg aactaaaaag cgatgttgtc gtcgatattt tgagacaatg
     4441 gctaaataca tcctctgtta aagatgatcc tcaggcttct tttaaattat cgcaaaggat
     4501 tcctgatgaa ttacttcaag aaatcgtaca agcaaacaag ctttatcgtg aggatttgtc
     4561 cgaaccggct aaatcaattg ccgtaatgga ggatttgatt gcaaaagatc gagtagacgg
     4621 aaatgcttat aatcgagaaa gtctattgca aatatttggt aagtataata ttcagctaga
     4681 ttctgatttt gatatggaaa aacttcaaga ggaattaaag tccataaaag ggcaaccgtt
     4741 agcagtttat actataacaa tgttgtttaa acgtatgaag cttggatttg atcgttttaa
     4801 aggtcgaaag caaccaaaag caactgctct gtttacagga tcaacagggg tcggtaaaac
     4861 tgcaatggct aaagccgtgg caagaggact ttatggcgat gaaaataaag ttctcttagt
     4921 taatatgccg gactacaaaa caaaagatga tgcaatgcgt tttagacgac tcataggtac
     4981 tcatgtaagg catgatcctt ctgcgattat tgtgtgtgat gaatatgaga aagctggtgt
     5041 agaaacactc gatgcctttt tatcaatttt agatgaaggt aggataagtt ttactgaaag
     5101 aggatgggat gatcgagaag ttcacgacac tgtttcattg tcaaatacaa ttttatttgc
     5161 gacaacaaat gcaggtgcgg agatgttcga ttcgttaaac agatatagct cactatctcg
     5221 tgttacaaac aaggaaaatt ctaatgaata tagcactgaa ctaaaaagag aataccgaaa
     5281 agtcgcatca gggatcaaac aagccttgat tggtgaaggt ctaaagcctg aagttattaa
     5341 ccgatttgga cgaatagttc cattttgggc tttatccgaa caaactcttc tggaaattgc
     5401 ttataatgaa atgttgaaga gtgctgaatt agtacgaaaa catttaaata tagatttacg
     5461 tcttccacct aggaaagaaa gagtatatgg tcaaaatttt tatgcagacg atctctccat
     5521 gcacttagtt tttgacatgg cttcacaaga ggattctaaa tcagggggtg caaggaatat
     5581 cttgcattta gttgaatctg agtttgaaga agcgattact gaagcaataa ttacttatcc
     5641 aaacgcaaaa atattcgatg ttaagttgag tgaagccgga gcgtttgaag gaaaaggtga
     5701 ttctaatgct gaaattattg caaagccttc cgttgctgaa agtaaagaaa acaagattgt
     5761 attttcgatt ggaggggcgt agctatgaaa ttcaagaaat atttactaca aaaccttaga
     5821 ataaaacgat gggtcgtaag aggtctagtt attggaggag tattagcagt tactttaggc
     5881 gctccttttg gaatgaatga tccaactcct gacaatcaag atagcaaaca agaaacagtt
     5941 ataaataaga acgttgcata tgcggaaagt aaaccggcag aacaaggaat gtggacacta
     6001 tatgctcgta tcctaatggg agataaagaa gttaatgata gtgccgctaa aactggacaa
     6061 atgaatttgt taggacaagg tggtatcagt gccgatttcc cttattctga catttctaaa
     6121 caaggtaatt cgattgcagg gggtaatgca ggtactcaac tagcatctac tttagcaact
     6181 tatagttact acggatatat tgaaacagtc tcaggaaatg ctattgccag tcaagcatca
     6241 tccattctag gaagtattgg taggacgatt ggtagtttta tcggatcaat tagtttaatc
     6301 ctttctaata ttgctgaatc cataaatgaa ctccttggaa aatttttcat gagtatgaat
     6361 atattttctc ttatggggtg gggagaaggt ggatcggatg tatctaatcc ggtatctaaa
     6421 gcattaacta gcttgcttaa caatttggga ttaaataatg aattatttaa agcgatagca
     6481 agcttatcta tgctcttttt cacagcaata ctaggttatc aaggaatgaa attattgtcg
     6541 aaagatggtg ttaactcgaa gggattatca ccagtacttg aaaaatggat tgttcgtatt
     6601 tttgtattgt ttatattctt accagtggca ggatcgctat tgggagttta tatcaacggc
     6661 atccagtcag caaaaaaaga tggagatata ccaacaaatg ttgtttctga ctacatcatt
     6721 aacacaagac tttgggcttc tactcaaaat cttgctccat catctgatac tggtggtgat
     6781 gtacctgacg gaaagcctac atccgaaatc aagttcgttg atacagatta tgaaccatct
     6841 aaaaaatctg caatgataag agcgatcaat actagcagtt atgccagaca aaataatgat
     6901 ctagattcta attcagtcgg tatggctcta ttaaaagaat ggtcatcgaa tgatactttc
     6961 aatattaata cttatgccgg tgatgttaat gtcgccgaaa atgatgctta tatgggaaga
     7021 gcgcctgctg tttggacagg ctataattta ggggatggaa aagaaggaag tgcaattaag
     7081 aaaccaacca atttaaatgg atatatttgg tcagcaaatc aaaatgtgac agacgacaat
     7141 cgcaagcctg atggaaaagg aacagcgcat aactttgatg ctactcaacc ttggggcgtt
     7201 gctaacaatg caacttttag cacacagtcg gtagctttat tgctccaatc ggcacttgat
     7261 acatcgggag ctaagtttta tgcgtataat atcgctcctt caggaattca aggcagtgct
     7321 aagaatatgt caacagtaaa aacagaatgg cgtacagcat ccttagttgg tcatggagct
     7381 atgggtaagt ttggctcgtg gctagcgatg aacgctgaat caatcgtttt agcagttctt
     7441 tacttagcat gtgcttgggc gatgttcaat gtgaatatcg tgaaagcatt taaacgtgaa
     7501 tttattcgga tttgggaagt agttctaaca ggatcacttg catcaggagc aggtgtgatg
     7561 gcaatttcat taggtgttgt tacaacatct ctgatcggac tacaattacc tatctttctg
     7621 attactttta ttcgtgcatt tggtgatact gctgtgggat tcattcctaa tacattagtt
     7681 ggcgatggcg tagcaaatat tgttcgatct gctatgattt taggtgtagg atatctttta
     7741 gtctttaaaa aaggcgaaaa agtaaataat ttgattaata tgcttattgg cttaccactt
     7801 gaagtagcgt ttgggtttga tgaacgtgct aagttattat ttggtaatag aaatgatgct
     7861 gtgcgagttg tggacggcgt ttctaaagca atcaagaaaa gccaaggagc taaaaatccc
     7921 tattatgatg gtggcaacag ctcagtaaaa gatatggttg caggaggtct tggagcagtt
     7981 ggagcgcaaa aaatggctga acgtttgaac aatatgggag atagcaatgc tgatgctaag
     8041 tccattgctg ataaagtaat tcctgaaaca ccagaaggaa tgtcattcaa cggtggcgga
     8101 gttggttcca caatcaataa tacctctttc aaagatttag ttcgtcaaga tcaactacgt
     8161 aataactccc aacaatatat tgatcattta ggacgtatgg ttgatgaaaa cggaaacttt
     8221 gtcgatgaaa atggtgaaat attaaaagat agtaaatacg gtaaagatgc taaaggcaac
     8281 tttattgacg aaaaagggcg tttggtcgat gaagatggaa accttataga tactaatggt
     8341 aaaccattgg aagatagtga agatggtaat attggtatgg atagcgaagg acgtttaatt
     8401 gatgaaaaag gtcgcttagt cgatgaagat ggaaatcttg ttgacgaaaa tggacgcagg
     8461 ttgctaaatg gtgattttgg tcatgatagt aacggacgtt tagttgacga aaaaggtcgt
     8521 ttagttgatg aagatggaaa tcttgttgat gagtttggta ataaacttgg taaacacagc
     8581 gatggatata atgtgaatgg agatttagtc gatgaggatg ggaatatcat tgagacgaga
     8641 gatgcgcttg tggcagatcg aaatagagaa ctcgaacaac aacggcatac gccaattgct
     8701 gttgcaattc ctgagaatga agaagacgag attactttag atcaaactca tcaagaactg
     8761 aggaatgaaa tgagtggtga ggacgtgtca gacagagtgc atcgggaatt aggagatgga
     8821 gcatctggtg aaagtcagtc aagagagctt aacagcaact ttgataatag tgctattcgg
     8881 aatgacacag tacttaaaga cagagaatcg ctaacaaaag aaacggataa agaacaatca
     8941 gatgtgaaga aaaagttcat tcctactcct aatgttaatc aaacagataa atatgaaaca
     9001 aatattggat caggaatgtc gaaagacaat caatcaatta gagaacctga actgacaaat
     9061 aaaaataata gaacaaataa ttctaaatct acttcagata attcagggaa atctaatcaa
     9121 tcaaccaaga aaacatcaac agctaaatca gccgttgaat atctatcaaa aactggtaaa
     9181 aatgtttaca cctcagcaga taaagcttta gcaaaagctc caaaaggtag tctagcaggg
     9241 ataacctatg aagctaaaaa cgaagctatt aaaataggta aaggaacggc taaggttgcc
     9301 aaggctactg ctacaggagc atccaaaact ggtaaagtag tgaagaaatc taccatgtca
     9361 gtcaaggact atattgtaaa atacggtaat caaaaatcaa ctgaaaataa gtctgaagac
     9421 gagtaaaaag aaggagcatt gatttgctcc ttctttttac tttgatttct aactgaagat
     9481 gtcaaggaaa atttctattt atgcaaaaaa a
//
LOCUS       DAZUMV010000108         9443 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000108 DAZUMV010000000
VERSION     DAZUMV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.109"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..205
                     /locus_tag="ACG8FK_002220"
                     /pseudo
     CDS             59..205
                     /locus_tag="ACG8FK_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            282..611
                     /locus_tag="ACG8FK_002221"
     CDS             282..611
                     /locus_tag="ACG8FK_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5097789.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            601..888
                     /locus_tag="ACG8FK_002222"
     CDS             601..888
                     /locus_tag="ACG8FK_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5097790.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1174..2031
                     /locus_tag="ACG8FK_002223"
     CDS             1174..2031
                     /locus_tag="ACG8FK_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5097791.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            2032..2580
                     /locus_tag="ACG8FK_002224"
     CDS             2032..2580
                     /locus_tag="ACG8FK_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097792.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2840..3301
                     /locus_tag="ACG8FK_002225"
     CDS             2840..3301
                     /locus_tag="ACG8FK_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFS5097793.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            3345..3431
                     /locus_tag="ACG8FK_002226"
                     /pseudo
     CDS             3345..3431
                     /locus_tag="ACG8FK_002226"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(3397..4467)
                     /locus_tag="ACG8FK_002227"
     CDS             complement(3397..4467)
                     /locus_tag="ACG8FK_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5097794.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            5069..6913
                     /locus_tag="ACG8FK_002228"
     CDS             5069..6913
                     /locus_tag="ACG8FK_002228"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5097795.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            6969..8435
                     /locus_tag="ACG8FK_002229"
     CDS             6969..8435
                     /locus_tag="ACG8FK_002229"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5097796.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            8472..9314
                     /locus_tag="ACG8FK_002230"
     CDS             8472..9314
                     /locus_tag="ACG8FK_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5097797.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         3246 a         1443 c         1906 g         2848 t
ORIGIN      
        1 agcttaaatc atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac
       61 ccaatcattt gagaagttaa aacctacaca tgaaggtagg tattttagaa gattgaatag
      121 acaacaccgg gttgtttata aggtagatga agatgaacac gtggttgaaa tttattcagc
      181 aaggacgcat tacgagtcac agtaattact tgtaaataca ttatctcgtg gtataatgta
      241 tttactaagt aattacatac tgttagaaat gagggattta aatgattaca aaaacaagaa
      301 aacaaggcaa ttcaatcatg ttgacagtac ctaaagattt taatgttcct aatggagtcg
      361 aagttgaagc aaagttggta gagaatggta ttctttatga atttgttgaa ccacaaaaag
      421 aattttatga ttttagcgaa gatattttgt ctgatataat tgcagaaggc tacgataaag
      481 atgagattct tgtagaattt aagaatagaa aaaacaaaat gcattcttcc tttagagata
      541 ttgctgaaga tacacttact aattctaaag taatgactaa agaggagttg gcaacagaaa
      601 ttggcttatg aaatcgttcc ttcaaaacat gtcattaagt atttaaaaaa gctaaaagag
      661 aagccattga aagaaaaatt tttgaattta atttatgatg aaattgcgat tgatccatat
      721 aagggtgaac aaaaaactgg ggatttatca ggtatttggt ctagtgggtt caaatatgcg
      781 ggcacaacat atagagtagc ttacgagatt atagaaaaca aggttattcc agtattgtta
      841 tgtggaacgc atgaaaactt ttatgaacag ttgaaaaaat taatctagtg atttgttgtc
      901 gttcttaaga aggacggctt cttttttatc gaaaatctga ttcgtctaca tgactaaagt
      961 cgcgagtatt ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata
     1021 aatcaatata tttcagcttt tccttattta tgtactacct tttacttatt aattataagg
     1081 tagtacatat atatcttttt tttggtagta actaaaaata aatatgttat aatagtatca
     1141 aatgaatatt gctaaaagaa aagaggtagg aacgtggtag cgaagaaata tgtaatcggt
     1201 aattttaaag gtggcgtagg taaaagtacg tgcgcgcaaa tgttagggtt tgaatcagca
     1261 gttaatgaag gaaaaagaac cttgataatt gacttggata tgcaaggaaa tacttctgat
     1321 gtgatgaact taacgcatat gaacttcact aacgaagaag gaggcggaga aggtgaaccg
     1381 attgtttacg aaaatactgt gactgacgtg ttgataaaca atcttccacc taaagaggct
     1441 gtctataaag taatcaataa cctctatata cttccagcag acatgtcctt tgaaatgtat
     1501 gatgattgga tcaaagacca atataatgat tcattatccc aattcaaata tatgaaagaa
     1561 aagctagaac cattatttga tgagtttgac gtcatctatt tagatgtccc accttctatt
     1621 tctgtatata gtaaatccgc gatgtacatt gcggattggg caatcgtcgt cttacagacc
     1681 caagtaaaat cgatgagaaa tgcgatgcaa tatcttgagt atatggactt ttttgtgaaa
     1741 gaatttgata caaatttata tgttgctggt gttattcctt tcatgttgga aagcggtgac
     1801 gcagtagatc aggaaatgta taaacaagcc caagagattt atggggaaca tttgattaaa
     1861 aatgttgtac tcaaaaatgc gcgcttaaaa cgatacgacg gatcaggtat tacgatggag
     1921 aagacaaaga aaggtctctt aaaacaatgg gatcgcaaaa gccacgaatt atttatcagt
     1981 attttgaaag aattggaaga acacgagaat tggtatgtgg aggtggagta attggctgac
     2041 atcaaaggac ttgatatttt tgggaacaac aaaggactcg ccaaaataaa aaaagaagca
     2101 gaagcaaaga aaaagcagca agcaattaca ccatctgaaa ataagcaatt agaagtgcac
     2161 caagttcaag aaataaaaac ttcctccaat gttgaaagac agagtagagg aagaacaaaa
     2221 aaagttggcg caccaatcag aaagtttgat aaggtttatt ccaccaagca tcctttaaaa
     2281 ctgtcgactt tgttaaatgc gacttccaga gttttagtag aaaagtatga gactaatatg
     2341 acaagagatg aattgcttag aaaagcttta gatgagtata ttaaacagaa tcttacgcaa
     2401 gaagataaac aagatttgtt taatgacgtg gtgaaagaac tagacttgtt cagagaaaaa
     2461 tatccaattg ttccagaatt agatgatcgt ggagaaataa taagaactac cagtcagata
     2521 aaacaagata ccgaagactc actgcgcaaa gggtggggaa tgaataataa agttagataa
     2581 taatcaaaga agaactaagt aactaccttt aaacggtagt tacttagttc ttctttgatt
     2641 tgcgtgaaat agaggggatt tatttttctg gttacgtgat taaaaaatcc aaaaaatgag
     2701 cataggaacg gttatgattt tagggtgtgt tttaatgaga tattgtgatt tcattaataa
     2761 gtgtatgata atctgttgtt gagttagtaa ataagtaata gagctaactc taaaataaaa
     2821 ctattaggag gagatgtaga tgaaaaagtt taaaaagaaa atatatttag gattggttgt
     2881 tggggtgagt atgtttgggt tcatggctca tactaaacaa gtaactgcag atactaacca
     2941 aaaagaaagg attatgagcc aattagatga ctctcaaata gagtatgtac ccaatgagct
     3001 aattgtcagt ttaaaggcta cacaaagaat ttcaacagag aaaaataaac cgcaaaattt
     3061 attgggtgta aaagttatgg tggaaaaaga tttgactctt aggacaaatg aaagcgatta
     3121 taataagcga gatcaagtat tattagtgaa attgaacgaa gaaattagtg tggaagaggc
     3181 agtaagaaca tttaatgctt ctgatatggt caattatgca gagccaaact atgtggttga
     3241 actagagccg ttgaatgatg ttgaggagcc aaatacaact gattctttag gaaatcgata
     3301 acgatcctaa ttggagtaaa caagctaatt taagagagat tagcatgcca tccgcttggg
     3361 aagaaaccaa gggttctaag gatgtgaagg tagcggtcat tgattcagga attgattatc
     3421 aacatgaaga tctttctgga aatgtagatg aaagtttggg atacgatttt ttttactttt
     3481 ttcaagaaaa taataattca gaatattctg actatattta tgcaacacta gtgactttag
     3541 gagaaacatc aatagccttg ctaaagtttt tgctttcgct aaagagagta ttttcgataa
     3601 tttgtttttt tctaaaatac ctcgttcctc aatatttggg atatggatat gtatagggat
     3661 tcttcgcata aaggtttcca gaaaatcctc tttgccttct gtagtcgcaa aaactaaacg
     3721 tacattggag gcgtgatgga tagtttcacc gactctagaa taagttccgc catccatata
     3781 aacaaatagt ttctcttgcc cttcaggagg caatctgtgt acttcgtcta aaaacagtac
     3841 tccaccgtct gcatcttgca acaatccatt tcgatcttca tctgcgccgg taaaagcacc
     3901 tttaacatag ccaaatagta tccccgacaa taattcagga ttattatggt attgcgcaca
     3961 attaagtact ttaaaaggag cattctcatg aataacgttc acttttttca taaactgata
     4021 agcagtatga acaaaaaaac tttttccaac tccagtatct ccagtgatca tcataggaag
     4081 ccctcttcca ggatagaaga cagcagtttt catttgttcc accgcatcgg ataagctcat
     4141 atcataccca atcatctttt gtagaatgga atctgtagaa ttgggctcca agtctgtttt
     4201 taattcagat atactttcgt atacatcttt taccggaatc aaaaagtgct cttcaaagcg
     4261 ctctttgatg gcaaataaca ctggacgagt attgatttta aatagaacgt tctttgcaac
     4321 taagtcattg gcaagtttac tggcatttgt tcttcccgtt cttaaaaatt gcgacaggtc
     4381 atttgtgtca aaaaagcact cttctaaatt actgatgcca atttcttcac attttgtgtt
     4441 tgcataagtc actatatcat ttttcatctt ataaacagtc ctttctaaac ttctatccct
     4501 taaataaata gcaaaaaaaa ggaagcaata tcgatatgct gtcctcttaa tttaataaag
     4561 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
     4621 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
     4681 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
     4741 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
     4801 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
     4861 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
     4921 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
     4981 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
     5041 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
     5101 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
     5161 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
     5221 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
     5281 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
     5341 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
     5401 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
     5461 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
     5521 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     5581 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     5641 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     5701 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     5761 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     5821 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     5881 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     5941 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     6001 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     6061 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     6121 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     6181 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     6241 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     6301 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     6361 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     6421 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     6481 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     6541 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     6601 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     6661 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     6721 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     6781 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     6841 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     6901 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     6961 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     7021 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     7081 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     7141 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     7201 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     7261 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     7321 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     7381 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     7441 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     7501 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     7561 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     7621 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     7681 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     7741 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     7801 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     7861 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     7921 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     7981 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     8041 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     8101 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     8161 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     8221 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     8281 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     8341 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     8401 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     8461 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     8521 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     8581 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     8641 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     8701 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     8761 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     8821 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     8881 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     8941 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     9001 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     9061 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     9121 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     9181 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     9241 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     9301 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     9361 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     9421 ttaacgtaag ataataaaaa atc
//
LOCUS       DAZUMV010000109         9381 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000109 DAZUMV010000000
VERSION     DAZUMV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.110"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..1042
                     /locus_tag="ACG8FK_002231"
     CDS             236..1042
                     /locus_tag="ACG8FK_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5097798.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="ACG8FK_002232"
     CDS             1054..2274
                     /locus_tag="ACG8FK_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097799.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="ACG8FK_002233"
     CDS             2264..3850
                     /locus_tag="ACG8FK_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5097800.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="ACG8FK_002234"
     CDS             3889..6027
                     /locus_tag="ACG8FK_002234"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5097801.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="ACG8FK_002235"
     CDS             6315..7706
                     /locus_tag="ACG8FK_002235"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5097802.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="ACG8FK_002236"
     CDS             7765..8574
                     /locus_tag="ACG8FK_002236"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5097803.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..>9381
                     /locus_tag="ACG8FK_002237"
     CDS             8546..>9381
                     /locus_tag="ACG8FK_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5097804.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQ"
BASE COUNT         3231 a         1442 c         1895 g         2813 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ctcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gatagcatcg tttatcagtc ttttaattca aaacagtatg ataatatttt
     9241 gcaaagaaaa atattagttg atgaagtaaa gaaagattat cctcgactta ttaatttggc
     9301 caatgctcga ttagtagacg gatacctttc aacagggttt aaaattactg gcaagaatca
     9361 aaatcagaaa gaaaagcaaa a
//
LOCUS       DAZUMV010000110         9363 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000110 DAZUMV010000000
VERSION     DAZUMV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.111"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..711)
                     /locus_tag="ACG8FK_002238"
     CDS             complement(43..711)
                     /locus_tag="ACG8FK_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5097805.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            845..1132
                     /locus_tag="ACG8FK_002239"
     CDS             845..1132
                     /locus_tag="ACG8FK_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097806.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1548..3086
                     /locus_tag="ACG8FK_002240"
     CDS             1548..3086
                     /locus_tag="ACG8FK_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS5097807.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3083..4048
                     /locus_tag="ACG8FK_002241"
     CDS             3083..4048
                     /locus_tag="ACG8FK_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097808.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4035..5357
                     /locus_tag="ACG8FK_002242"
     CDS             4035..5357
                     /locus_tag="ACG8FK_002242"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5097809.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5420..5524
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5598..6341
                     /locus_tag="ACG8FK_002243"
     CDS             5598..6341
                     /locus_tag="ACG8FK_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5097810.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6356..7348
                     /locus_tag="ACG8FK_002244"
     CDS             6356..7348
                     /locus_tag="ACG8FK_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5097811.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7360..8097
                     /locus_tag="ACG8FK_002245"
     CDS             7360..8097
                     /locus_tag="ACG8FK_002245"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097812.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8087..8809
                     /locus_tag="ACG8FK_002246"
     CDS             8087..8809
                     /locus_tag="ACG8FK_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5097813.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8893..>9363)
                     /locus_tag="ACG8FK_002247"
     CDS             complement(8893..>9363)
                     /locus_tag="ACG8FK_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS5097814.1"
                     /translation="LINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEA
                     PVQAEQPVVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYT
                     ATNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3070 a         1610 c         1976 g         2707 t
ORIGIN      
        1 atgatatttc tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc
       61 aagaggatca agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac
      121 agatccaatt tccagatact tcactttatt caatgtataa gatagaatag aacagctaag
      181 taatgtatac agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa
      241 agtaatcaag aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata
      301 aatgatatct gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc
      361 agaaagaata cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg
      421 aaggctctcc cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag
      481 aaaagcgtgt tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag
      541 tgaagtgaca gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac
      601 cccaccatct gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc
      661 tgcgcctaaa atgattccgc acaattctgt aaataaattt aattttttca tttctagcct
      721 cttttctttt gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa
      781 gaggaaaatg aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg
      841 aaaaatgcga gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta
      901 tcaaggagtt tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa
      961 taaagcaaat caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga
     1021 catctttctt tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga
     1081 actcattcct ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat
     1141 aagaaaacaa gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt
     1201 gtcatgatac agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat
     1261 ttctacttta ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat
     1321 agctgattcc aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag
     1381 aaccaaaaag ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg
     1441 tatttttttc ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa
     1501 aaggaaagaa atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta
     1561 tacaaacatt aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca
     1621 tttctctcat ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag
     1681 ccaatcataa aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc
     1741 cttcacttgc cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat
     1801 tgattgcact tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag
     1861 ctgaaattga cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac
     1921 gcttgctgaa agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg
     1981 ccttagtttt aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag
     2041 ggacatttat tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga
     2101 tcagttctgc cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga
     2161 aacctcgata ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt
     2221 tatatcagtc tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg
     2281 aacctgaaat cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag
     2341 ttgaattaaa accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc
     2401 aaatcgatat ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg
     2461 aagcaatgaa aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat
     2521 tgaataagca attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct
     2581 tggcagtcaa gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag
     2641 ctatctctga tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat
     2701 ataaaggaat ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa
     2761 gtttgcgtta ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag
     2821 atagccagct caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag
     2881 cttatcaagg cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta
     2941 aagcactgaa taaattaagc ggaaaaataa cagaggatca aatgattgat atgaattacc
     3001 aagtcaatgt cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag
     3061 gattagtaaa ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa
     3121 gtatgatgga aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt
     3181 tgttttggtt ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt
     3241 atctaaacca tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct
     3301 gcagaaaatg cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat
     3361 gacagtcaag caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag
     3421 agcagaccgt gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga
     3481 tcgtatgcct agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat
     3541 tgctgcaagc ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc
     3601 aagaaactca ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt
     3661 tttcgttacg cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa
     3721 ggatggagaa attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga
     3781 ctacgttcgg catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt
     3841 agtcgtcaat ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt
     3901 tgatacgcct atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt
     3961 agaaaagcaa agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg
     4021 agaggaggaa ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct
     4081 taagtgctgc ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc
     4141 tatggggcgg gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag
     4201 tagaggcacg taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg
     4261 ccaactggga agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc
     4321 gaaaaaaaca attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg
     4381 atgattcatc tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa
     4441 ccgggcaaag acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag
     4501 actttttatc attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg
     4561 cttttgagct agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata
     4621 atcaacggcc attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg
     4681 aagcatctgg cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta
     4741 ctcgctatcg tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga
     4801 ctgggagaca gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata
     4861 aacacggaat tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg
     4921 gagatattgt ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt
     4981 atgtagctaa gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta
     5041 cgatcgtcta cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt
     5101 cgtcagatca agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg
     5161 aaccagttgc taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag
     5221 tgattagcga gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat
     5281 tgactaaaaa agagttggat agctatatca tggggtatcc gacattggca agtgatctct
     5341 cctatttatt gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg
     5401 ctacacttta aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt
     5461 tgtgaactta gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga
     5521 cgcttgaagc gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga
     5581 agaaaggagg taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt
     5641 taattatttg gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac
     5701 ctgctgctgt attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca
     5761 cgacattaaa tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg
     5821 ccttggcaat ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt
     5881 cacaaatgtt gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta
     5941 tgcttccaaa agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt
     6001 cggatacatt gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag
     6061 atacagggaa gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg
     6121 gtttaaaagt ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg
     6181 ctcaagcagg cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag
     6241 tttttgctgc gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat
     6301 tattgaaaaa agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa
     6361 aaaattatgg cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga
     6421 aacaagtagc aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt
     6481 accgaatact aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc
     6541 tggtttaaat gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg
     6601 tgcggacaaa gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga
     6661 tggacagaat ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt
     6721 cgccacccta tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc
     6781 aggttggcaa agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg
     6841 ggacttttca aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa
     6901 atcctcagat atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg
     6961 tattgaacta aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc
     7021 ggtaattatt acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat
     7081 ggatgccacg aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact
     7141 gttgcaaaaa tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt
     7201 gtcaaaaaat tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg
     7261 caattataca gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga
     7321 actattcact aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac
     7381 ttgaatgtag tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa
     7441 gataccttta catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact
     7501 ttaacaggag tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact
     7561 ggtctccata cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc
     7621 gtctatcaaa acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa
     7681 gtaagggatt atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa
     7741 ttatctggcg ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct
     7801 tacttgcttc ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa
     7861 gactggatgt tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat
     7921 atcgaagaag caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat
     7981 gtgattgctg agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa
     8041 tcagagttga agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc
     8101 tcattgtcat ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt
     8161 gacgatcatc aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag
     8221 taagactcaa gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga
     8281 acaagttgag ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat
     8341 ttggacgaat gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc
     8401 agccataaat gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa
     8461 ttatatccaa gcttacccta atcactattt gatccattgg tattcttctt tggactatca
     8521 acaagactat attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc
     8581 agcagtctgg caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg
     8641 actacatgca gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact
     8701 gcttgaagaa cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa
     8761 aaaaaatact tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt
     8821 gacacaacta gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt
     8881 aataaatgaa tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga
     8941 accataacgt gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc
     9001 accatttaag taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt
     9061 gtaagaaccg cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg
     9121 agtagcagca gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg
     9181 tttttccgtt tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg
     9241 aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg
     9301 ttgtcctgag taaatcaggt taatatcaga aattgaattt gattctgcaa tggcattgat
     9361 caa
//
LOCUS       DAZUMV010000111         9082 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000111 DAZUMV010000000
VERSION     DAZUMV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.112"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..279)
                     /locus_tag="ACG8FK_002248"
     CDS             complement(<1..279)
                     /locus_tag="ACG8FK_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5097815.1"
                     /translation="MSITKNIKCTDEKTSHSIKQILNIKDSHITFSKDAISKERVHGR
                     MANVFTGLLTGEAHRCECCGFDSVIRHAYHDSWIQLLPYQEVPTYLHLY"
     gene            complement(394..624)
                     /locus_tag="ACG8FK_002249"
     CDS             complement(394..624)
                     /locus_tag="ACG8FK_002249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097816.1"
                     /translation="MIEINEKILVFDKKKDDKDNDSYDDFNSGTLILDATCAPQNIKY
                     PTDTELMNDTRTHAEKIIDVVCEGKKLKKPVL"
     gene            complement(714..803)
                     /locus_tag="ACG8FK_002250"
     CDS             complement(714..803)
                     /locus_tag="ACG8FK_002250"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097817.1"
                     /translation="MTFGALLIQKEYSYSDEETVLQIQENPYL"
     gene            complement(800..985)
                     /locus_tag="ACG8FK_002251"
     CDS             complement(800..985)
                     /locus_tag="ACG8FK_002251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097818.1"
                     /translation="MYKLQTTVQLAFEDFNQPIGLKMNPSNRWIQKAELIPWAELEKD
                     YAKNFRKKKETSQNPFA"
     gene            1154..3265
                     /locus_tag="ACG8FK_002252"
     CDS             1154..3265
                     /locus_tag="ACG8FK_002252"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5097819.1"
                     /translation="MNTKDLKLLETIVRSHVTNSQQLKSLVHFSTRQITYSIKKINDE
                     LSLHGLSLIKRSNSGEWLYEANDLQKLLIYMNQAMVFKEGAIPEYINDDLAYSEIRRY
                     SELLFILANPKKVTTQEIMAFIGISKNTARNDFSKIKKELPPDYTLIFDPQNGYMIES
                     SMAKLHTLLLEVFNYLFERHATVNRFESLFSEEREFVGFFIHTYEQTFKLKFSDNSLT
                     KLYYLIGLSLSSFKRRTKGQIDFSYDLQELDEFQMIRSCIDQHYQHAEIAIDYEWLTL
                     LFLSFNTISNEFEILDSRIYQVIMEMITLFQSKSAIRISNKEELTNRLMNHLRPAIYR
                     IRYELPFEIDDLAEIVKENHEVSAVLNFVKESIVPLEAFLRKTIPETELYLISVYFCS
                     ELLNSSIIETPVRKKAIVVCSNGLIMANVMAKTLKEIFPDIQFVASSSVREMDKFVED
                     VDLIFSNQPLKSEIPLYIIQPIMNEEEKNRLRNRVLIDFSTNNISKETRNLTEIIKRY
                     SAVQNEESLQTDIERFLINYANSLIYLDRQSKQAQKISFVELLVSEGIILVSTKQLTW
                     QNAMELSCQPLLKEQVINQNYQTILMSQMADEEAYYFLNNRIAIPHSSPENGVIKGGC
                     SLLISVDPIIFPGNRPIHFISPFAIGKKENYLLAINQLLQLSKDEEYQQEILLCTTED
                     EVRKKIKNFLRRKQYVNNEFS"
     gene            3243..3893
                     /locus_tag="ACG8FK_002253"
     CDS             3243..3893
                     /locus_tag="ACG8FK_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS5097820.1"
                     /translation="MSITNFPELTIILRGYSFEEAMFVMEELAAMNTSVGIEVTTNND
                     DFARIITEGNKKFGQQLLIGAGTVLTFSQADQAIAGGAKFLLGPKKFSTPIFSLAKER
                     GVVTVPSAFTPSEIQVMFDEGADIVKVFPAVTVGPTYFKQLAGPFGIRPLMAVGGVTI
                     DNIAAFKKAGVVYFGVGSSMFQKADIQNRNGQAIRKSIQSYLDKLAEAGSEEDESH"
     gene            3880..4332
                     /locus_tag="ACG8FK_002254"
     CDS             3880..4332
                     /locus_tag="ACG8FK_002254"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5097821.1"
                     /translation="MSLIKLEEKNILLNQSFSNQEEYFQKITKKLMTENMVKEGYLES
                     ITKRETTYPTGLYIGERSIAIPHTDYQLSNVTAILVSVLKDSLHFQRMDDPEEMVSVN
                     VIIHLLFDSPDKQPQLLKELMSKVTNQDFLQAIMEAKSKTEVLTAFNQ"
     gene            4349..4654
                     /locus_tag="ACG8FK_002255"
     CDS             4349..4654
                     /locus_tag="ACG8FK_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HFS5097822.1"
                     /translation="MSKKTIIVACASSMITSTMAANKIRKIAKTNGLPEPRILQLKFS
                     EVAGALESNQVDFIVPTGKLQENVTKGIKVIPGTPFVTGVNEEKSESLVLEALKELD"
     gene            4666..5943
                     /locus_tag="ACG8FK_002256"
     CDS             4666..5943
                     /locus_tag="ACG8FK_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5097823.1"
                     /translation="MNIVIDALNWISSLGPMIMMPIIILILGLFFRIKLNTLLKSALT
                     VGIGFAGVNIIINWFISQVGPSVQLMVKNWGIQTSIMDVGWPARAAATWSFPLAAVMV
                     FIILGINVLMLIFKKTNTVMVDFWSYNHYIFVAALVYYATDKNIILAVIFGAIDAIIS
                     FKLADWTTPLVEDYFELEGVNFPTANSVGWAPLAWLLNKLWDRIPGLNKVDARPEKIQ
                     ERFGFVGEPMFMGFVIGLVIGLLAKEPVDKVLIVGMSTSATMVLTPKMMQILMEGLLP
                     FANSVKKFLNVKFPGNNFTMGVDAALTVANSSAIATGIIMVPITLVLAAILPGNKLLP
                     ISDIAYQAMWLSAWPVAFSKGNIIRGILSTTIITACVLLIATSLAGIHTQLALAGGFQ
                     LPAGMTQISTEDAGTHIIGFILMKIAEFISNIF"
     gene            5962..7110
                     /gene="dgoD"
                     /locus_tag="ACG8FK_002257"
     CDS             5962..7110
                     /gene="dgoD"
                     /locus_tag="ACG8FK_002257"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFS5097824.1"
                     /translation="MKISKITVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRFLVGQNPFAIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKALNVPV
                     YELLGGAARDKIKVYSWIGGDRPSEVVEQAQDRVDRGFTAVKMNATEELHYIDSYEKV
                     AEVVERVASIRERFGDTLSIGVDFHGRVHKPMAKVLAKALEPYHPMFLEEVVLPENEE
                     HFSEVANAVAVPLATGERLYTRWQFKSILQQGAIDIIQPDVALCGGILETRKIAAMAE
                     AYDIAVAPHAPYGPIALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEIFQ
                     YKDGFVDLPTKPGLGLEMDEDRVKEIAQEGLVWTNPKWKNYDGTIAEW"
     gene            7122..8024
                     /locus_tag="ACG8FK_002258"
     CDS             7122..8024
                     /locus_tag="ACG8FK_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HFS5097825.1"
                     /translation="MINTVTGPIEAESLGTTFIHEHLYVIPNELRKYEDYTLDDIYHS
                     IAEVMAFKQAGGKTIVDLTPINYGRNPLALKMISEATGVNVAFVTGFHKEEFQPKWLT
                     TLNDDEIFELLYSEITHGVTSQNLLPGAMKVGTSKNEVTLAEQRILAIAGQVQKKTGI
                     PIITHCDGGTMGLEQINCLTKNGAKASNICLSHVDLTKDFHYLAQICETGASICMDHI
                     GRELANHDADRVQLIKRLVDAGYENQICLSGDMGRKKYLPTYGGTPGLTYILTSLKEE
                     LLKVMSEPTFNKMIEMNPINILSK"
     gene            complement(8693..>9082)
                     /locus_tag="ACG8FK_002259"
     CDS             complement(8693..>9082)
                     /locus_tag="ACG8FK_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5097826.1"
                     /translation="FKQFPLFQGKYMTESEVLDYLLSIDNQLRQSYEVYQELLAAFDA
                     KDFTEFFNLIESLPHSLNDGFKKAICYLKKHTEAITNSLKYPYSNGKLEGKNNLIKVI
                     KRIAFGFRTFRHLRMRVLIQQNICEII"
BASE COUNT         2947 a         1397 c         1855 g         2883 t
ORIGIN      
        1 gtacaggtgt agataagttg gtacttcttg atacggaagc aactgaatcc aactatcatg
       61 ataagcatga cggatgacag aatcaaaccc acaacattcg caacgatgcg cttctccagt
      121 taataagccg gtaaatacat tggccatacg gccatgaacg cgctctttac taatggcatc
      181 ttttgaaaag gtaatatggg aatctttgat gttcaaaatt tgtttgatgg aatgacttgt
      241 cttttcatcc gtacatttga tattcttagt tatggacatt tggttatcct cctatgattg
      301 tttttgccga tttaattata ggacaaccag atgtcttttt gttataaaaa aatatttggc
      361 gtcactactc agtttgagta acaaaaccaa atattataga accggctttt tcagtttttt
      421 cccttcacaa acaacatcga tgattttctc ggcatgggta cgggtatcat tcattaattc
      481 tgtatcggtt ggatatttga tgttttgtgg agcacaagtg gcatccaaaa tcaatgttcc
      541 ggaattgaaa tcgtcataag aatcgttgtc cttatcgtct tttttcttgt cgaagacgag
      601 tattttttca ttgatttcta tcaacgttgt ttcgtccaag cgtttccgga aatagaccat
      661 tgtagagcat cgaaaggctt ggcatcttga tagcccggca aaccgatgaa gaactataaa
      721 tacgggtttt cttgaatttg taagaccgtt tcttcgtcgc tatagctata ttccttttga
      781 atcagtagag ctccgaaggt catgcgaaag ggttttgcga cgtttccttt tttttacgaa
      841 aatttttagc gtagtctttt tcaagttcag cccatggaat gagctctgct ttttgaatcc
      901 aacggttaga cggattcatt ttgagaccta tcggttgatt gaaatcttca aaagccaatt
      961 gaacggttgt ttgtaattta tacatgaaaa gcctccaaaa ttgagtggta ctccacacca
     1021 ttctgatggt gcataagata aaaaattaaa gaagtgcctg tcatttgtct gatttaagcc
     1081 aaggtttgac aaattttgga tattccaatt aatactgtaa gcggttatat ttaactcagg
     1141 gaggtagatg aatgtgaata caaaagattt aaaactgtta gaaaccattg tacgatcaca
     1201 tgtgacaaac tctcaacaat tgaaatcatt agtacatttt tcgactaggc agataactta
     1261 ctctataaaa aaaattaatg atgaattatc ccttcatgga ttgagtctga taaagcgaag
     1321 taactcaggg gagtggttat atgaagccaa tgatcttcaa aaacttttga tatacatgaa
     1381 tcaagcaatg gtttttaaag aaggtgcgat tcctgaatat ataaacgatg atttggctta
     1441 tagtgagata agaagatatt cggaactgtt atttattcta gccaacccca aaaaagtgac
     1501 aacccaagaa ataatggcgt ttattggtat cagtaaaaat acggctagaa acgacttttc
     1561 taaaataaaa aaagagttgc caccagacta cactttgata tttgatcccc aaaatggcta
     1621 tatgattgaa tcaagcatgg caaagcttca tacattgctc ttagaagttt tcaattattt
     1681 atttgaaagg catgcaacag ttaaccgttt tgagtcactc ttttctgaag aaagggaatt
     1741 tgttggtttt tttattcaca cctacgaaca aacttttaaa ttgaaatttt ctgataattc
     1801 actaaccaaa ctttattatt tgataggttt atcactttct agttttaaaa gaaggacgaa
     1861 aggacagatt gattttagtt atgatttaca ggaattagat gagtttcaaa tgattcgaag
     1921 ctgcattgat caacattatc aacatgctga gatagctata gattatgaat ggttgacgct
     1981 tctgttttta tcattcaata caatttcaaa tgagtttgaa attttagatt caagaatcta
     2041 tcaagtaatt atggagatga ttaccttatt tcaaagtaaa tcagctattc ggattagtaa
     2101 taaagaagag ttgaccaatc gattgatgaa tcatttacga cctgcaatct atcgaattcg
     2161 ctacgaatta ccatttgaaa tagatgattt agctgaaatt gttaaggaaa accatgaagt
     2221 tagtgcggta cttaattttg ttaaagaatc cattgtacct ttggaagcat ttttgcgtaa
     2281 gacgattcct gaaactgaat tatatttgat ttctgtttat ttttgttcgg agctattaaa
     2341 ttcttctatt atcgaaacac ctgttagaaa aaaggcaatc gtcgtatgta gcaatggatt
     2401 gattatggcc aatgtaatgg cgaaaacatt gaaagaaatt tttccagata ttcagtttgt
     2461 tgctagttct tcggtcagag agatggataa gtttgtcgaa gatgttgatt tgatttttag
     2521 caatcaacca ttgaaaagtg aaattccatt atatattata caaccgatta tgaacgaaga
     2581 agaaaaaaac cgcttgcgga accgtgtact cattgatttt tctacgaata atatcagcaa
     2641 agagactagg aaccttactg aaattattaa gcgttactct gctgttcaaa atgaagaaag
     2701 tctacaaact gatattgaga gatttttaat taattatgca aattcactaa tatatctaga
     2761 tcgtcaaagt aaacaggcac aaaagatatc ttttgtggaa ttgcttgttt ctgaagggat
     2821 tattcttgta tctacgaaac aacttacttg gcagaacgcg atggaattgt cttgtcagcc
     2881 tttattaaag gagcaggtaa ttaatcagaa ctatcagact attttaatga gtcaaatggc
     2941 ggatgaagag gcttattatt ttcttaataa tcgtattgct attccacatt catcgccaga
     3001 aaatggggta attaagggcg gttgctcgtt acttatatca gttgatccaa ttatttttcc
     3061 tggaaatagg cctatccatt ttatttcccc ttttgcaatt ggaaagaagg aaaattattt
     3121 acttgcgatt aaccaattac tacaactttc taaggacgag gaatatcagc aagaaatact
     3181 tctttgcacg acggaagatg aagtgagaaa aaaaataaaa aattttttaa ggaggaagca
     3241 atatgtcaat aacgaatttt cctgaattaa ccatcatttt aagaggatat agttttgaag
     3301 aggcgatgtt tgtcatggaa gaattggctg cgatgaacac ttcggtagga attgaagtaa
     3361 caaccaataa tgatgacttt gcacgtatca tcacagaagg caacaaaaaa tttgggcaac
     3421 aattattgat tggtgcaggg acagttttaa cattttcaca agcagatcaa gcaattgctg
     3481 gtggtgcgaa atttttatta ggaccaaaaa aattttcaac accaattttt tcgttggcta
     3541 aagaacgtgg ggtggtaact gtgccatcag cttttacgcc atcggaaatt caagttatgt
     3601 ttgatgaagg tgccgatatt gtaaaggttt ttccagcagt cactgtaggg ccaacctact
     3661 ttaaacaact ggccggtcct tttggtatac ggcctttaat ggcagttgga ggagtgacga
     3721 ttgataatat tgcggctttt aaaaaagcag gagttgtgta ctttggggta ggatcaagca
     3781 tgtttcaaaa agctgatata caaaaccgca atgggcaagc tattcgaaaa tcaattcaat
     3841 catatttaga taaactagct gaagcaggaa gtgaagaaga tgagtctcat taaattagaa
     3901 gaaaaaaata ttttgttaaa tcaaagtttt tcaaaccaag aagaatactt tcaaaagatt
     3961 acaaagaaac tgatgacgga aaacatggta aaggagggat atctcgagtc aatcactaag
     4021 agagaaacaa cttatccaac ggggctatat attggggaac gctcaatcgc aattccacat
     4081 acagattatc agttatcaaa tgttaccgct attttagtat ccgttcttaa agatagtttg
     4141 cattttcagc gaatggatga tcccgaagaa atggtttcag tgaatgttat tattcacctt
     4201 ttattcgata gtcccgataa gcaaccgcaa ttattgaagg agttgatgag taaagttacc
     4261 aatcaggatt tcttacaagc aattatggaa gcaaagagta aaacagaagt tttaactgca
     4321 tttaatcaat aaattggagg gaaatacaat gagtaaaaaa acaattatcg tcgcatgtgc
     4381 atcatcaatg attacatcta caatggcagc taataaaatt cggaaaatag caaaaacaaa
     4441 tggattacca gaacctcgaa ttttgcaact aaaattttca gaagttgcag gtgctttaga
     4501 atcaaatcaa gttgacttta tcgttcctac agggaaatta caagaaaatg taaccaaagg
     4561 aattaaagtt atacctggaa caccatttgt tactggagtg aatgaagaaa aatctgaatc
     4621 tttggtgtta gaagcattga aagagcttga ctaggagggt gaaaaatgaa tattgtcatt
     4681 gatgcgttaa actggatctc aagtttgggt ccgatgatta tgatgcctat cattatcttg
     4741 attttaggat tattttttag aattaagcta aatacattac taaaatcagc gttgactgtg
     4801 gggattggtt ttgcgggtgt aaatattatt attaattggt ttatttcaca ggtaggtcca
     4861 tctgttcagt taatggtcaa aaattggggt attcaaacaa gtattatgga tgtcggctgg
     4921 ccagctcgtg cagcagccac ttggtctttt cctctagcag cagttatggt atttattatt
     4981 ttgggtatta atgtgttgat gctgattttt aaaaagacca atacagtaat ggtcgatttc
     5041 tggagttata atcactatat ttttgtagct gctttggttt attatgcaac ggataaaaat
     5101 attatattgg cggtaatctt cggggcgatt gatgcaatta ttagttttaa actcgctgat
     5161 tggacaacgc cgttagtaga ggattacttt gaactagaag gggttaattt cccgactgcg
     5221 aactcagttg gttgggcacc cttggcttgg ctacttaaca aattgtggga ccgcattcct
     5281 ggattaaata aggtagatgc tcgtcctgaa aaaattcaag agcggtttgg ttttgttggt
     5341 gagcctatgt ttatgggatt tgtcatcggc ttggttattg ggttattagc aaaagaacct
     5401 gttgataagg tattgatagt gggaatgagt acttctgcaa caatggttct aacacctaaa
     5461 atgatgcaaa ttttaatgga aggattatta ccctttgcta attctgttaa gaaattcttg
     5521 aatgtaaaat ttccaggaaa caattttaca atgggtgtgg atgctgcgtt gacagttgct
     5581 aattcaagtg ccattgctac gggtattatc atggttccga ttaccttagt gttggctgct
     5641 attttaccag gtaacaaact cttacctatt tcagatattg cctaccaagc aatgtggcta
     5701 tctgcatggc cggtcgcctt ttcaaaagga aatattattc gcggaatact ttccaccacg
     5761 attataacag cttgtgtgct tttaattgct actagtttag ccggcataca tacacaatta
     5821 gctttggctg ggggattcca attaccagca ggaatgacac aaatatctac cgaagatgca
     5881 ggtacacaca ttattggttt cattttaatg aagattgctg agtttatttc aaatattttc
     5941 taatattcag gagggcatac agtgaaaatt tcaaaaatta cagtctataa agtaaaacca
     6001 agatggattt ttgtcaaaat atctacagat gaaggtattg aaggctgggg tgaaatgatt
     6061 tctggaacta agactgaaac agttgtggca ggagcttatg agataggaaa tcgattctta
     6121 gttggtcaaa acccatttgc aattgaacgt ctgtttcagg aaatgcatcg ttcatttttc
     6181 cgaggtggtc ctattaatgg aacgattgtc tctggacttg aaatggcttt atgggatatt
     6241 aaagggaaag ccttaaatgt tccagtttat gaattactag gtggagcagc ccgtgataaa
     6301 atcaaagtat attcttggat tggtggagat cgtccttctg aagtagtaga gcaggcacaa
     6361 gatcgagtag atcgtgggtt taccgccgtg aaaatgaacg cgacagaaga attacactac
     6421 attgattctt atgaaaaagt agctgaagta gtggaacgtg ttgcttcaat tagagaacgt
     6481 tttggagata cattgagcat tggtgtcgat tttcatggtc gtgttcacaa accaatggct
     6541 aaggttttag caaaagcact ggaaccctat catccaatgt tcttagaaga agtggttttg
     6601 ccggagaatg aagaacattt cagtgaagta gccaatgcag ttgctgtacc actagcaacg
     6661 ggtgaacgtt tgtatacaag atggcaattt aaatcaattc ttcaacaggg agcgattgat
     6721 atcattcaac cagatgtagc tttatgtgga gggattttag aaacaagaaa aattgctgca
     6781 atggctgaag cttatgatat agcagttgcc ccccatgcac catatggacc gattgcgtta
     6841 gcagctacat tacaagttga ttcttgtaca cctaatgtgt ttattcaaga acaaagctta
     6901 ggtattcatt acaacaaagg gtttgatttg ttagattttg taaagaataa agagatattc
     6961 caatacaaag atggcttcgt agatcttccg acaaaaccag gtttagggtt agaaatggac
     7021 gaagatcgag tgaaagaaat tgcacaagaa ggtttagttt ggaccaatcc taagtggaaa
     7081 aattatgatg gtacaatcgc tgaatggtag gagatgagtg catgatcaat acggttacag
     7141 gcccaatcga agctgagtct ttgggtacaa catttattca tgaacattta tatgtcattc
     7201 ctaatgagtt aagaaagtat gaagattata ccttggatga tatttatcat agtattgcgg
     7261 aagttatggc ttttaaacaa gctggtggaa aaactatcgt tgatttgaca ccaattaatt
     7321 atggacgaaa tccgttagct ttgaaaatga tatctgaagc taccggagtg aatgtagcat
     7381 ttgttacggg attccataag gaagagtttc agccaaaatg gttgacaacg ctcaatgatg
     7441 atgagatctt tgagttgctt tattccgaaa taactcacgg agtcacttca caaaaccttt
     7501 tgccaggagc aatgaaagta ggtacgagta aaaatgaggt tacattggct gaacaacgaa
     7561 tactagccat agccggtcaa gtacaaaaaa aaacagggat tcccattatt acgcattgtg
     7621 atggggggac gatgggactg gagcaaatta attgtttaac aaaaaacggt gcgaaagcaa
     7681 gtaatatttg tttgagtcat gtagatttaa ctaaagattt tcactattta gctcaaatct
     7741 gtgaaacggg agcaagtatt tgtatggatc atattggccg agagttggcg aatcatgatg
     7801 cagaccgcgt ccaactaatt aaacgactgg ttgatgcggg ttatgaaaat cagatttgtc
     7861 tttcaggtga tatgggacga aaaaaatatt tgccaactta tggaggaacg cctgggctta
     7921 cctatatttt aacaagtttg aaagaagaac tattaaaagt aatgagtgaa ccaactttta
     7981 ataaaatgat tgagatgaat ccaattaata tactgagtaa gtgaagtcaa ttgaatcatt
     8041 cagaagaact aatgaaatta gttgctttcg cagggttaat tcttctctct gggtttggaa
     8101 aagagggctt agcttcggat tccctagtta aactaggggt tttatcaggt tattgtactg
     8161 attataagta cctgaattat tcatgaaata ctttttccgt cgttttatag gcagttgggt
     8221 caataaaaag atcagaaaaa cgaacgagag actctcataa cgtgccacta aataaaaatc
     8281 gtacgttatt ttaaaaaatg ggttgattct aggcagtact ttgtcggtct atttttagaa
     8341 aaagaggttc tttgtcaaac atacatgaat tcgcaagctc attcaccctg aaggatgaaa
     8401 ggcagaaact gtagtatact aatggtggaa aggaaaaaaa gccgccagca atttggaaaa
     8461 gaacgttatg tcgattgtca aactggcttg ctttatttct cgagaaccct gaaatatagc
     8521 caatgagcat gtgtataaaa aattgatttt atatgaaata aagcactcct ttcctaaaat
     8581 tattggaaag gagtgcgttt tttatggaaa ttatttaggt tctttgtcaa ttgctattgt
     8641 tttgctcaaa tcgtatgtta ggaaatgctt cggcatttcc ttttttgcgt tattaaatga
     8701 tttcacagat gttctgttga atcaggacac gcatccggag atgacgaaaa gtccgaaatc
     8761 caaaagcaat tcttttgatt actttgatga ggttattctt tccctccaat tttccgtttg
     8821 aatagggata ttttaaggag tttgtgattg cttctgtgtg ctttttcaga taacaaattg
     8881 cctttttaaa cccatcattc aaggaatgtg gcaatgattc aatgagattg aaaaattcag
     8941 taaaatcttt tgcatcaaaa gcagctaata actcttgata gacttcatag ctttgtcgta
     9001 gttggttatc tatagagagt aggtaatcaa gcacctccga ctccgtcatg tatttccctt
     9061 ggaagagggg aaattgctta aa
//
LOCUS       DAZUMV010000112         9047 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000112 DAZUMV010000000
VERSION     DAZUMV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.113"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..1027
                     /locus_tag="ACG8FK_002260"
     CDS             125..1027
                     /locus_tag="ACG8FK_002260"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS5097827.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            1240..3015
                     /locus_tag="ACG8FK_002261"
     CDS             1240..3015
                     /locus_tag="ACG8FK_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS5097828.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3081..4667)
                     /locus_tag="ACG8FK_002262"
     CDS             complement(3081..4667)
                     /locus_tag="ACG8FK_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS5097829.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            4917..5141
                     /locus_tag="ACG8FK_002263"
     CDS             4917..5141
                     /locus_tag="ACG8FK_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS5097830.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            5283..7034
                     /locus_tag="ACG8FK_002264"
     CDS             5283..7034
                     /locus_tag="ACG8FK_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS5097831.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            7034..8818
                     /locus_tag="ACG8FK_002265"
     CDS             7034..8818
                     /locus_tag="ACG8FK_002265"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097832.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKTEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
BASE COUNT         2921 a         1631 c         1855 g         2640 t
ORIGIN      
        1 ttagagaaaa tttcagttac tatggagaac agggcaaaga aaaatcaatc attttctttg
       61 tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa ggaggaatcc
      121 atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt taggagcagc
      181 agaagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg cccctcaaaa
      241 cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt acatggcaga
      301 aaaaggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg ggaatacgat
      361 caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg ttcgtgcgga
      421 agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg caggaacaga
      481 agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag aacagacggc
      541 acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa catttgagga
      601 attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta cttttgacac
      661 ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg taatggaaga
      721 gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg attctaaaaa
      781 tccgcaaggt tcgcataaag accggcacgc caatattggt tttggaacaa tcggattcga
      841 cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa tcttggaaac
      901 tccctatgta ggcaaagata aaaagaaagc ttacgcacca tatggaaaag aaatcgccat
      961 gttcaaagcg caaacattcg atccagaagt gtttcctgag ttattagcag aagaagtgat
     1021 actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc cttgtccttt
     1081 tttgtttaag aaacaaagga gtactttttg cagtcttttt catcatgcat cttctccccc
     1141 ctctttttaa atttagacaa acaaggtata ctggtagaaa agtaaaaaaa cgaaaagttt
     1201 cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac tttaaaaccc
     1261 ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc cctattattt
     1321 acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat gtcggcaacc
     1381 tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa atggctaggg
     1441 gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca
     1501 cataaactgc atctttctta tgcagttttg ttcggcatgt tcagtatttt tgccagtata
     1561 ttggttgcac tcgcgtttaa accgattgtg aagaacaagt atcttctttt aagtatctat
     1621 ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt
     1681 aatgccctcg tgattccggc tgttgagatc attatcgctt gtttattagc tatttattac
     1741 cgcaaatacg gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt
     1801 cctttctttt ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct
     1861 ttgggtatca gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag
     1921 aagttaaaaa aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt
     1981 ttacttccta tagttggctt ttccatcgtc tatcaatgga tcgaatataa aaatgagaca
     2041 acatacggta ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa
     2101 caactgatcc gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc
     2161 tcacatgctg cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc
     2221 cgtattgacg aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta
     2281 cccggagata tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa
     2341 aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc
     2401 tatcacaagg gaacaatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga
     2461 aagacgatga gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca
     2521 tggcgatata aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc
     2581 aaacaggctc aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa
     2641 ccgagaacac ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa
     2701 atttaccaag ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg
     2761 atactaggaa ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg
     2821 gtcaccggat taatactgac acaattggtt tttcttttcg gtgtctcatg gttttgttct
     2881 tatgcgccag aaaaaaagga ctatttttta agtgtctaca ctggtgcagg cgtgccatta
     2941 acgcaattca tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaagct
     3001 ttatcttcta aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct
     3061 ttgtgtggaa aaatcagttt ttattcgatt tcttctaccg tttcctgcaa aagctgttca
     3121 tattttttgt ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga
     3181 acaatgatta cataagcgat gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg
     3241 ccgaagaaac tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc
     3301 acagagaacc agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc
     3361 gcttggctgt tggtcatcat cagtaaagga atcatcgaaa acggcaacgc aaaagccaag
     3421 aacacctggg attcattcat taagttattc aaagcggcat gttcttcgat cgttcctttt
     3481 gtacttgtaa acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt
     3541 cttcttagcc aaatcgggat acgcatatgg atgaatcctt ccatgatgac ttgaccggtc
     3601 aacgtaccag taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg
     3661 gacaagatac cagattttgc tacagcaatc aaaataccat tgctcatagc tgatggatca
     3721 gataaagctt catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca
     3781 gcgacaccca tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta
     3841 gaatcccaag tagaaaaacg tacagcttgt gctacatctt cttcgttttg gtgatcgatc
     3901 ttccgagttt gtgaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca
     3961 ataatcccta aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat
     4021 gtatgactgt tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca
     4081 acttgataga caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc
     4141 ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag
     4201 acggcaatca ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact
     4261 tctgcaatat ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacctaat
     4321 gtcttacttg tgcgtgctcg gattgcctgt gccaaatcca tttggctcac gattcctaat
     4381 tttgctgcca tatactgtaa aagcatagca atcagactag acatcaaaat aactgacatc
     4441 aataaatatt ggaaattttg tcctccggtg atcgaagtag accagttacc cgggtccata
     4501 tacccaactg ctactaacgc accaggtcca gagtaagcaa gaagtgtttt aaaaaaactt
     4561 ttcccctttg gtacatcaac tgttccattt acttcagcta aagataaacc atttgtatgt
     4621 tcaataagtc gatgcttatg aaattgttta ttggattgat tattcaagaa aaaaccacct
     4681 ttcctattta tctacgatta cattctacac ctcaaaaaga aaaaattaaa gaaaaagatt
     4741 cggtacgcct aatttttttc atggcgcgtc ttgctagatg acgaaagaaa ttttgttctc
     4801 ctctaaaaaa accactattt tttgcatgaa actaaagaat acgcctttct tttttcaata
     4861 aactttagta aaataagtca ttgagagcaa ttcggaaata gaaaggagag atttatatgt
     4921 tatcaacagg tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg
     4981 gctttttcct agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg
     5041 aggacatgtt gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc
     5101 gccagatgat gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caacgacttt
     5161 ttacgggctg attttttcag tccgtttttt atttcgtaat ttgttagact attcagatta
     5221 ttcagcaaaa aagacaagca cagacaatcc taggctcttg ataaagacaa aggaggagta
     5281 aaatgtcgat ttttagaaaa ttaggctggt tttttcgtca ggaaaagaaa cattatttaa
     5341 ttggggtttt ctcgctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg
     5401 ggatcatcat tgatgagatc gccgataaaa atttatcaat aaagatcatt ttcgggtgga
     5461 tcttcctatt gattatcgct gcttttttac aatatctttt tcgttatatc tggcggacga
     5521 atatttgggg aagtgcagct cgattggaga aagaacttcg gcagcagtta ttcaaacatt
     5581 ttacagaaat ggacaatctc ttttaccaaa aatatcggac tggggatttg atggctcacg
     5641 caacgaatga tttgaatgcg atccagaatg tagcaggagc tggtatcttg acctttgcag
     5701 attcagtgat tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct
     5761 taacactgat tgcgctgatt cctctgcctt tgctggccgt cacttcacga gtgctggggt
     5821 caaaactcca tgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga
     5881 cacaagaaag tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc
     5941 tagcggattt tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt
     6001 tagactcctt atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa
     6061 ttattttagg tggaaagtat gtcttggaag gcacgatctc gatcgggcag atggtctcat
     6121 ttattagtta tatcggtatg cttgtttggc cgatgtttgc gattggtcga ttgttcaacg
     6181 tattggaaag aggaaatgct tcttatgatc gcgtcaatga acttttgcat gaaaagacgc
     6241 atatcgtgga acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta
     6301 tttcctcttt taattatccg aaagaagaat caccagcatt agaacaaatc aagtttgcga
     6361 tccaagaagg agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacgatca
     6421 tcaaattact gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt
     6481 cgatcaaaga atattcattg aatgctttat taggatcaat cggctatgtg cctcaggatc
     6541 acttcttgtt ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag
     6601 aagaagaagt agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattac
     6661 caaaaggtta tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac
     6721 aaaggatttc gattgctcga gcattgatca aaaatcctga attattaata ttagatgatg
     6781 cactctctgc agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa
     6841 gaaaagagaa aacaactatc attgcagctc accgtttaag tagcgtgatg catgcaaaag
     6901 aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg aacccatcat gagctgcttg
     6961 cactaggcgg ctggtatgaa caaatgtggg agaaacagca attagaagcg aagatagaag
     7021 gaggcgaggc ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa
     7081 agaacagaaa aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt
     7141 catcgttgct atcttatttg cgtttgcttt gtctgtgatc aatgtcctat tgccaaggat
     7201 catccaaact tttatggatg atcatctggc taaacagtcg gctacgacac aagttatttt
     7261 gttttttgct ggtatgtact tatttggagt catagtaaaa agtatcattt ggtttttcca
     7321 atggtatttg tattccatgg cctcattgaa gacctatcag tatattaggg taaaattatt
     7381 tgaaaagctg catacactag gtatgcgtta ttttgatcag acaccggcag gatcaaccgt
     7441 ttctcgagtg acgaatgaca cagaaacgct atttgaattt tggtacgtct ttttgatggt
     7501 attgactggg atctttgcag tcgtttcttc attccttgcc atgtttcaaa tcaatggaga
     7561 aattgccttg tggactttgg tgtttttgcc gattttagga atcgtcatct ggtattacca
     7621 gaaattcagt tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa
     7681 attgaatgaa tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt
     7741 agctaaagaa tttgaacaag tgaacaatga gtatttagac acgcgttatg cgatgatcaa
     7801 aacgaattcg attctgttaa gtccgatcat caattttcta tatgcattgg cgattgcgtt
     7861 ggtgttgact ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta
     7921 tgcatttacg acctatgtcc agggattttt taatccaatg tctcaaatga tggacttttt
     7981 gagcatcttc acagatggga tggttgctgg tagtcgtatc ttgaagatta tggatacgga
     8041 agaatatgca ccacaacaaa accctggagc agatcaaatg attacttcag gtaagactga
     8101 gtttcgcaat gtaagctttt cttatgatgg agagcacaat gtattaaaca atatctcttt
     8161 tgttgctaat ccgggagaga ccgttgcatt agtgggacac accggaagcg gaaaaagctc
     8221 gatcatcaac gtattgatgc ggttttacga attttatgaa ggtcagatat tgattgatga
     8281 tcatgacatt cgagaatatc caatggaaga gttacgtaag aaaatggggc tggtcttgca
     8341 agatgctttc ttgttttatg gggatatcgc aggaaatatt cggatgatga atccagctat
     8401 cacagacgaa cagatacgcc atgcggctga gtttgttcaa gcaaacaaat tcatcgatga
     8461 attaccaaaa ggatatcact caaaagtgat cgaacatggt gccagctatt ctagtggaca
     8521 acgacagctg atcacttttg cgcgaacaat cgtgactgat cctaaaattc tagtattgga
     8581 tgaagcaaca gcaaatatcg atactgaaac agaaggattg atccaagaag gattggcaaa
     8641 aatgcgtcaa ggacggacaa cgattgcgat tgcccatcgg ctgtcaacga ttcgagatgc
     8701 agaattgatt ttagttttag acaaaggtca tatagtagaa cgcgggaatc atgaaacact
     8761 tctagcgcaa aatggtttgt atgcagatat gtacaaactg caaagcagtg gtatataaaa
     8821 gatgttgtga cagtagaatt ttgacctaag cactagagtg aaaataaaac atagcttctc
     8881 atatttttca tctgtgagag ttagtgtcgt agtcaccaga tttaaaaaaa gattcgacag
     8941 ctagtcagaa gtgcctaata gccactaaga aagcgttgac aaaagcattt gctttcgata
     9001 atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagc
//
LOCUS       DAZUMV010000113         8991 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000113 DAZUMV010000000
VERSION     DAZUMV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.114"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1390)
                     /locus_tag="ACG8FK_002266"
     CDS             complement(<1..1390)
                     /locus_tag="ACG8FK_002266"
                     /inference="COORDINATES: protein motif:HMM:NF014437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5097833.1"
                     /translation="MSNKEEAKSIINFVGGKENIAALTHCVTRLRFALRDDTKVDKAG
                     LENLEDVKGTFNANGQFQVVIGPKVADIYEEVISQSGLSGANKEDVKKVAAELNLNWF
                     QRAVKILGDVFVPILPAIVTAGLLMGLNNLLTNPGIFFEQSLIEVYPNIADVADMINL
                     IANTSFQFLPALITWSTVKYFKGNPLLGIVLGLVFVHPDLMNAWNYGQAVTTGDIPHW
                     TIFGLSISKIGLQAQVLPALVSGYVLAKTELFLKKRVPETIQLLVVAPVAILFTSVIT
                     FIIIAPITIAIGNTIVAGIVWLFQKVPFIGGFAYGIGLAPLVVTGMHHAFLPVHIQLM
                     ANGGDYIWPIGVMSSLAQGSAAIAMNFLIQNKKLKGVGLAAGLSAYMGITEPAMFGVN
                     LKYKFPFVVSILTSGIIGGFMASQHVIATSVGISGIPGFLSIPFEFWPIFFICMLASC
                     VVSFGATYLYGKN"
     gene            complement(1383..1685)
                     /locus_tag="ACG8FK_002267"
     CDS             complement(1383..1685)
                     /locus_tag="ACG8FK_002267"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HFS5097834.1"
                     /translation="MTTTVSNKGSRDGYEVVQLYIGAQYGRYIRPTKDLVAYKKVFVK
                     SGDSVTVSFELTNKDFKYYDEEFNEIIDDGAYWIMVGNDSSQALTNKEEIEVRINE"
     gene            complement(1755..1994)
                     /locus_tag="ACG8FK_002268"
                     /pseudo
     CDS             complement(1755..1994)
                     /locus_tag="ACG8FK_002268"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
     gene            complement(2838..5987)
                     /locus_tag="ACG8FK_002269"
     CDS             complement(2838..5987)
                     /locus_tag="ACG8FK_002269"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFS5097835.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(6000..7217)
                     /locus_tag="ACG8FK_002270"
     CDS             complement(6000..7217)
                     /locus_tag="ACG8FK_002270"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS5097836.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(7207..8802)
                     /locus_tag="ACG8FK_002271"
     CDS             complement(7207..8802)
                     /locus_tag="ACG8FK_002271"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS5097837.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         2646 a         1854 c         1453 g         3038 t
ORIGIN      
        1 tatttttccc gtaaaggtaa gttgctccaa atgagacgac acaacttgct agcatacaaa
       61 taaagaatat tggccagaat tcaaacggaa tagacaagaa accaggaatt ccactgatac
      121 caacagaagt tgcaatgacg tgttgtgacg ccataaatcc tccaattatt ccagaggtca
      181 gaatggaaac cacgaatggg aatttatact ttagattaac cccgaacatt gctggctcag
      241 tgatacccat ataagcagac aaaccggcag ccagtccaac acctttcaac tttttatttt
      301 ggattaagaa attcattgca atagcagcag aaccttgtgc caaacttgac atgactccaa
      361 taggccaaat ataatctcca ccgttcgcca tcaattgaat atgaactggt aaaaacgcat
      421 gatgcatccc tgtcacgact aatggcgcaa gaccaatacc ataagcaaat ccgccaataa
      481 atggaacttt ttggaataac catacaatac ctgcgacaat cgtattacca atagcaattg
      541 taatcggcgc tataataata aatgttataa cactagtaaa aagaattgca actggtgcaa
      601 caaccaacag ttggattgtt tcaggcacac gctttttcaa gaatagctct gttttagcta
      661 acacatagcc ggaaactaga gctggcaata cttgtgcttg tagtcctatc ttagagatgg
      721 acaatccaaa gattgtccaa tgtggaatat ctcccgtggt tactgcttgc ccgtaattcc
      781 aggcattcat caaatctggg tgtacaaaga ctagacctaa cacaatccca agaagaggat
      841 ttcctttaaa gtattttaca gttgaccatg ttattaacgc aggcagaaat tgaaaggaag
      901 tgtttgcgat aagatttatc atatcagcga catcagcaat attaggatat acttcaatga
      961 gtgactgctc aaaaaagatt cctgggtttg ttaataaatt attcagtccc atcagtaaac
     1021 cagctgtaac gatcgctggt aaaatcggca caaatacatc acccaatatt ttaactgcac
     1081 gttgaaacca attcaaattc aactcagccg ctactttttt aacgtcttct ttgtttgccc
     1141 cactcagccc agattggctt ataacctctt catagatatc tgctacttta ggaccgataa
     1201 caacttgaaa ctgtccatta gcattgaagg tacccttcac atcttccagg ttttccaatc
     1261 ctgccttatc aactttagtg tcatctcgca aggcaaacct cagacgtgtc acacagtgag
     1321 tcagtgctgc aatattctct tttccaccaa caaagttaat gattgattta gcttcttctt
     1381 tattactcat taattcttac ctctatttct tctttattag ttaatgcttg agatgaatca
     1441 ttgcctacca tgatccaata agcaccatca tctataattt cgttgaactc ttcatcataa
     1501 tacttaaaat ctttgtttgt aagttcaaaa gaaacagtca cactatcccc tgacttaaca
     1561 aaaaccttct tgtaggctac caagtctttt gttggtctta tataacgacc atattgagct
     1621 ccaatatata gttgaacaac ctcataacca tctctacttc ctttatttga aacggtagta
     1681 gtcaccttca aacagttctt acccttctct ttaatgctaa ttggtgacat agaaaaagtg
     1741 gtataactca acccataccc aaagggaaat tgtggttcat tggatacatc taagtatttg
     1801 gtcgtattaa acttttcact tgaatacggt ctaccagtat tgaaataatt ataataaata
     1861 gggatttgtc ctaaatgtct cggaattgtt attgtaagct ttccggctgg gtttattttg
     1921 ccacaaagga cgtccaaaat agcattgcca gcctctgctc ctagatgcca agcctcaact
     1981 aatcccttac atatagattt ttaaattaca cactagttta aactaaaaca aacaaggaaa
     2041 aacttaccat ttaatcaaaa aattagctca tatgaatata ttagaatgta aattagactg
     2101 gtcaaaatct ggtcaaaaaa gaggacgaat acaggtagtc ctcttttttg ttattttgat
     2161 attctaaatc attttttatt ggattattcc cttgttcgat atagacccaa attgatctat
     2221 caataattat caaactgagt catatagaga caaaaatttc aaaaatgacc tgttaaatgg
     2281 tcatcgaata tcatttaaaa tcaatacgac ggagtcagac acggagtcaa aacaagcccg
     2341 ctgagagtaa aaaatataca gtcccatagg taaagtgcca caggactgta tattttttta
     2401 tttgagatta cctgaaatga ttccatttaa tttttatctc attttttcct actagttagt
     2461 atcaaatact ctcctatttt ctcaataaac tattttgggt tacatggtat ctgaaacagc
     2521 taaaaccttg tcattatagc aaaaaatcac tttatctccg tttcatttca gatacatttc
     2581 acttaagcgg attccaaaaa aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt
     2641 attggttcct ctcaaaacac ggtcatataa caacggggtg gggtaaatga atgaaaaagg
     2701 tggaggaaac attattcaat agctgtctta aatacttcgt tataaaatgt gaacaaataa
     2761 aagacatcta tccctatttt ctacacttcc acttgactac catctttgaa tcgtacaatt
     2821 ttcttaactc ttctcgttta cctcaattca ttctcaagtg gaatcaactc atcatctaac
     2881 attttacgcc atgctcgctt catttcttgt gggtatttaa atggcttcgc ttctggatgt
     2941 ttggctttat atccttcgta atccttactg ttgataattg ctttcatatt aggaattgga
     3001 tcttcacctg gtagatattg cagattagaa gcatgtaatt ctttctcaga aacaaaccac
     3061 tcattggaaa agacatgaat caaatcatca ctcgcttgtg taaaatagcg acgtttaatg
     3121 acaaagatat cttcattact atctaaagtc cccacatcaa atgcatttaa aatttgtcgg
     3181 taaacttctt tgactccttc tgttttattc aatttagcca aagctttttc aatttcttct
     3241 cgtgcatctg aactattcgc agcataagtt cctagtaatt gatcaatata agttgaatca
     3301 atatcataca acttagtggt attttcacta taaaattcga tcgttgaaaa atcttctcct
     3361 tctttttctg gttcttcctc tattaatgaa cccttcacgg tctcgtacac gccaatttgt
     3421 tcttccaaaa gttgaatttg attgttcaaa gttggagatt tcaccatgtc atcattgtaa
     3481 tcgtcataag tcactaacga ttcataagca cgattcaatt cttgaaatgc tttgacgtac
     3541 tcgatacgga tttcaagtgg cgtatgttca tcaatggatt ccatggatga aacctctttt
     3601 aacttaccat atgcttgttt gaatcttttt ttggattcct gataacttgg ataaatcaaa
     3661 cctgattctt cttttgcttt tgaatacagt tttgtcgcat cctcgacatt cttctccatt
     3721 gtcgctggct tacgaaacgt cacaatcaat ccttttgtct tatcaggata cgtacgattc
     3781 gtacgtgaaa aagcttggat caatcctgca tactctagat tacgatccac aaatagggtt
     3841 tgaatcgtag gggcgtcaaa acctgttaat agacgatcca ctacaatcgc taaatcgatt
     3901 tgtttcccaa attgtttgaa ttccgctctt tttcgtgcca aacgattatt gatgtctccg
     3961 ttataacgct caatgtctgc taatgaccag gaagtatcat aatactgatt atattcctca
     4021 ataatttttt gcatttcttc ttgttgggca cttgaatctc ggctattttc ttctaatgaa
     4081 taggtaatag cgattcgtgg aaaatcagga tcctccatcg ttcgtccttc acgaatgagc
     4141 tgattgggaa actcttttgt taaccaatca ggctctttcg tcatctcttt gatggcccga
     4201 taatactttt tggccatgtc aatggaactc gtggttaaga ttgcagattt cgttggcctg
     4261 ccatttcgaa aatcaaattt tgtataggca ttatctggac ggaacatttt atgaatgact
     4321 ttttggatat gttcatctgc ttcaaaaaca gctgggtcca aataactttc ttttttgaca
     4381 ggttccattt gatcaatcat acgattgatc tgttctgatg aatactctgc atatgtttct
     4441 acttctttta atttccgata gattttattc tctagtgaag tcggctccac ggtgttttca
     4501 tgctctactt gaaatcctaa gacagagcca tcttctagcg cattcttaat ggtataggta
     4561 tgcaagacct ctccatactg atcatgggtt gtacgtgcca attgcccttt ggcttgtttc
     4621 cgattctcag gaaaaatcgg tgtacctgta aagccaaacc aagtggattt cggaaacatt
     4681 tttttgattt cttccatatt ttcagcactt aacgctcgat gacactcatc tactacaaac
     4741 acgatatgtt ggcccattaa tttttggaag cgattggttc cttttttctc ttcttgtttc
     4801 tttgcatatt tcaacgccgc atctagtttt tgacgggttg taatgattac aacatttgca
     4861 ttggcatctg ccaacagggt ttcacttaat tctttggcac ttcccgttcc aacgatcaag
     4921 ctattcgctt tggcgttccc ggaagaaatc ccagtattaa attccgaagc aaattttgta
     4981 aactctgtgg tggtttgatt atctaaatct ttgcggtcca ctaacatgat cgttcgatcg
     5041 atccccgatt ttcgtgctaa aagcttagta gatacaaaac tagtcaacgt ctttcctgaa
     5101 ccagtcgcat gccacacata ccctgattga tgtttatttg cggcaataaa caaggcttga
     5161 atcgcatgta cttgataagg atgtaacacc atgagggttt tattgtcttg atcttcactg
     5221 acgattgtat aatccgcaat caaacgatga gcatccggga tatttaacac ttgtttacaa
     5281 aactcatata agttttctac tttttcattg tctttcgttc gccaactaaa taaaaactta
     5341 gggtgcaagt ctttcggtaa ggcatttgca aaataccgag tggtttgttc attggaaacc
     5401 acaaacaact gtaaggtcga aaagatattg ttccgaaaca taccttcttc tgcatatttt
     5461 ttgatttggt taaatgcctg ataaacacca tcttttgcag ttacttgttt cagctcgatt
     5521 tggacaatcg gtaatccatt gattaataag gtgacgtcaa aacgtcgatc ccgtgcttct
     5581 atgttacttc cacgttttgc aatttgatgg accacttcat aagtagagat tcctccacca
     5641 atgtcttgat tggaataaag gataagtgag acagaaccca attgcgaatc ttcccgttca
     5701 atcgtgatcc gagccatccc attttcacct tttaaccatt tcgcagcatc aaatggggtt
     5761 ttcgttcgca gcagtaattc cgttttgatc gtttcaaatt ctttatcggt caaaggaaaa
     5821 tcattcagtt ctgcttgatt atttgagata attttttggc gtaaattgac ccaaaggtct
     5881 tcttctgatt ttagatccgg acgatacgtc cactgattat gcccttctcc aagtacacga
     5941 atcaactgtt cttcgacttc tgcttcctca cggtgactaa ttttcattct tcttccctcc
     6001 tagacaaaca tcttttgtaa gaagcttttt ttcgtttctt ttagtaaatc taacttacgt
     6061 tgatgaagag cgatcgtatc atctagctgt ttgaagaaag taccgatttt ttgttgttca
     6121 tcctttacag gaaccttaac actgatattc tcaatatcag aactgttata agaaggcaat
     6181 gcccctactt gggagtatct tggcaaattt atagtttcaa acaatatttt accaaaatag
     6241 atatcccaag tattattaaa taaataagcc atggtattgt tatctattaa gaaagggctt
     6301 gtgaccaaac gttttctatt aagcattatt gctgcgccaa ctttagcaaa tataatcgct
     6361 ggcacatctt ttattggctt ccatttttta cgctcaattt gctcattaga tacataatga
     6421 tttgcatttc tcatttcata ctcattacca atgttattca tgtcggaaac tttaaaaaat
     6481 ggagtacctt cagaaccacc ttgctcggca tcaggaaatc caattcccga ttgagtttta
     6541 cctaattctc ccaacttacg ctcttcccaa tcttccgtaa aaccaggaaa ccggatttct
     6601 ggcacttttg ctccgttttc agggaacatt ttttgtaaga atcctttttt cgtttccttc
     6661 agcaaatcta acttacgctg atgaagagcg atcgtgtcgt caagttgttt gaagaaggtg
     6721 ccgatttttt gttgttcttc ctcactagga aacattgcta ctatattttt aaactttttt
     6781 agagacagaa catgttgacc tgttccctct gcttgaatag ataattgtcg ttggattcca
     6841 ttgttaatac gtcttaagaa gaagtcggaa tcagcagatt ttaaagtaaa tacaggataa
     6901 aaatagctta taatccctct atcaataatg tcattacggt taaacacaaa cacatcatta
     6961 tcacttcgac tacggaacgt gaaatatcct ctaggtagta caaagtaacc aatattattt
     7021 tctgtagtta cttgccgatt tgcaaaatag tcttcttgca aaactatccc tttttgttga
     7081 gatgaagtta gcataggaaa ttgattttgt acacttgtct tttcaacata atctacaacg
     7141 aagtccccca acttcctctg ttcccaatca tctgtaaatc ctgggaaacg gatttctggt
     7201 tgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg cttcattttc
     7261 aggtgttgtt tgcagggaag agatagcttc taaaagtttt ttttctagca cttgctgttc
     7321 ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt cttcttcttc
     7381 gaaagtatct acatagcgtg ggatatttaa gttatagtca ttttctttga tttcatcaaa
     7441 ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt cgaggatttt
     7501 ttgaatgttt tcttccgata gtttattttg atttttccct ttaacaaatt cacgactagc
     7561 atcaatgaat aatacatctc gagtttgacg attcttcttc aacacaatca ccgtcgttgg
     7621 aatcgatgtc ccaaagaaca agttcgcagg cataccgatc acagcataaa tactgccatc
     7681 ttctagtaat ttttgacgga tgaccccttc tgcagcgcca cggaataaaa ctccgtgtgg
     7741 taagacgatg gccatggtac ctgtttcctt caaatgatag aacccatgaa gaagaaacgc
     7801 aaaatctgct ttggattttg gcgctaattt cccataacga ttaaaccgag aatcatctaa
     7861 gaaagtcgta tctgctgacc aattagccga ataaggtggg ttcattacta ctgcatcaaa
     7921 tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc ggagattcat
     7981 ttcttcggcg tcgacaccat gtaaaatcaa gttcattttt gctagattgt atgtcgttgt
     8041 atttagttct tgcccatgat atttaacgtt atctgggtgt gttaagtagt tacgtacgtt
     8101 taacatcaat gaacctgaac ccatcgttgg atcaaaaaca ctaaagaatc gtcgttcctt
     8161 ttgatcgagc gttacgattt gcgccatcat atcagaaacc atatgaggtg tgtaaaattc
     8221 tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaactcat acgcatcccc
     8281 gatcacgtcc ccatcgtgtt ctaaaacatc tacatcattc aatttcttga tgacttcggt
     8341 aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga gatccacatc
     8401 atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg ctttgtttag
     8461 atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga aaagatactc
     8521 tggtgcaatt gcatatccca aaatatcaac gattgtggca atcagatctt cttttgattc
     8581 ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt catattcctc
     8641 taaggattca tctgctaaaa gaaccacttg ttccaacaat cgatcggata aatatttata
     8701 gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac tacggagatt
     8761 atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac actcctatct
     8821 cttatttaaa gttgctatat accctcttat tttagcatac ccccttttat aaaaagcaaa
     8881 aaaaaaaaga aagaaactta ttcttgctaa taaactacgc gaaaggagtc taacgttgtt
     8941 tgatataact tagactgatc gttaacttat tttttatatt tctacatagt t
//
LOCUS       DAZUMV010000114         8769 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000114 DAZUMV010000000
VERSION     DAZUMV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.115"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..2139
                     /gene="treP"
                     /locus_tag="ACG8FK_002272"
     CDS             151..2139
                     /gene="treP"
                     /locus_tag="ACG8FK_002272"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS5097838.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            complement(2224..2784)
                     /locus_tag="ACG8FK_002273"
     CDS             complement(2224..2784)
                     /locus_tag="ACG8FK_002273"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS5097839.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(2806..4914)
                     /gene="mutL"
                     /locus_tag="ACG8FK_002274"
     CDS             complement(2806..4914)
                     /gene="mutL"
                     /locus_tag="ACG8FK_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS5097840.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            complement(4932..7577)
                     /gene="mutS"
                     /locus_tag="ACG8FK_002275"
     CDS             complement(4932..7577)
                     /gene="mutS"
                     /locus_tag="ACG8FK_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5097841.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            complement(7561..7974)
                     /locus_tag="ACG8FK_002276"
     CDS             complement(7561..7974)
                     /locus_tag="ACG8FK_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5097842.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            complement(7952..8749)
                     /locus_tag="ACG8FK_002277"
     CDS             complement(7952..8749)
                     /locus_tag="ACG8FK_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS5097843.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
BASE COUNT         2250 a         1923 c         1653 g         2943 t
ORIGIN      
        1 tttttaagaa aagcttattt taagcttttt aaaacgatat cattttctct cgtacttttt
       61 aataaaacgc ttgcgattct ctccgaaaaa aagtatagta aatgaagaaa caacttgcat
      121 agacaagtta agagaaaggg gtttattgat atggcaaaat atcaagcaga tgccgagaag
      181 ctgttacatg atatcggcgg aaaagaaaat attgctgcag ttagtcattg tgcgactcgt
      241 atgcgttttg tcctgaatga tccaggaaaa gcggacgaaa aagcgatcga agatatacct
      301 agcgtaaaag ggatgttcac gaatgcagga cagttccaag tcatcatagg aaacgatgtt
      361 tctacttttt ataacgactt cgttgcagta tctggagttg aaggggtttc caaagaacaa
      421 ggaaaagcag ctgcaaaaca aaaccttcat cccgttcaac gtgctattgc cgttttagca
      481 gaaatcttta caccactgat tcctgcaatc attgtcggtg gattgatcct tggattcaga
      541 aatgtacttg aaggtatcca gtttgaaagt cttggtggaa caatcgttga acactcgaag
      601 ttttggaatg gtgtcaatgc ctttttgtgg ttaccgggtg aagcaatttt ccattttctt
      661 ccagtaggta tcacttggag tattgcaaaa aagatgggta ctacacaaat attaggaatc
      721 gttttaggaa ttacgttagt ttctccgcaa ttattaaatg cttatagtgt agcttccaca
      781 gccgcagcag atattccatt ttgggatttt ggttttgctc aagtccagat gattggttat
      841 caagcgcaag ttattccagc catgctggca gggtttatgt tagcgtatct agaaatcttc
      901 ttccgcaaat atatccctca gtcgatctcg atgatttttg ttccattgtt ttcattgtta
      961 ccaactgttt tagctgcaca cgttatttta ggtcctatcg gctggacagt cggaagctgg
     1021 atctcaaata tcgtaaatac tggattgacg tcttctatca gctggttgtt tagtgccgta
     1081 ttcggattcc tgtatgctcc tttagtgatt actggtctcc accatatgac caatgccatc
     1141 gatatgcagc tgattgcaga tttcggatca acgaatttat ggccgatgat tgctttatca
     1201 aatatcgccc aaggctctgc agtcttagcc atcatcttct tacatcgtgg gaataaaaaa
     1261 gaagaacaaa tctcgattcc agcaatgatt tcttgttact taggcgtaac tgaacctgct
     1321 atgttcggaa tcaatttgaa atacgtctat cctttcgtcg ctgcaatggt cggctctgga
     1381 ttagctggca tgtttgctaa cttgatgggt gttcgagcta acgcgatcgg agtaggcggt
     1441 ttgccaggaa tactagctat ccaagcagaa acatgggtac ctttcatcat cgcaatgatc
     1501 attgctgtca tcattccatt tggcttgacg atcatcttta gacgtcaggg aattttgaat
     1561 aagattgatc ctgctgttcc tgaaaatgct gctgatgtac aacttcaaac agcaaatggc
     1621 gcaacagcaa cgccacagtc tttcgaacct gtttcagcaa ctggtacggc agttgcaaca
     1681 aaagaaacat tattcgcagt cgctgcaggg accatcaagg aaataactga agtaaatgac
     1741 ccagttttct cacaaaaaat gatgggtgac ggttatgctg tcgaaccgtc aaacggcaaa
     1801 gtctatgcac cagtaaacgg taaagtgacg agtgtctttg aaacaaaaca tgccattggt
     1861 attctttcta atgaggggct ggaagtactt gttcacatgg gattagatac tgttgaacta
     1921 aagggtgttc ctttcaatgt attcgtgaaa gaaggatacc ttgtcacacc agagaccctg
     1981 attgctgaaa tggatttgcc agaaatagaa caagctggca agaaaactga cattattgtt
     2041 gctttgacca ataatgaaaa agttgcagga ctttctcttg accaatcagg acttgttcgc
     2101 ccgggcgaag cagtcggtaa agctgaagta aaatcttagt cagagtagat aagtaatctt
     2161 ttcaataaaa aaaacgggct atcatggatt ttcaatcgaa aatcagatag tccgcttttt
     2221 tgtttaaatg aatgttttca gtgcctgatt gacagcacca atcggaaatc ctacgatact
     2281 gtaataatct ccagaaattt ctttgacgaa tacactcgca gcattttgga tgccgtatgc
     2341 ccctgcctta tccgcataat cccccgtctc caagtagcgt ttgatctctt cgtctgtcaa
     2401 aggataaaaa gtgacatgtg cttctgctag tatccgttct tctcgttcct ttgtttttaa
     2461 aacaacagct gtatatacag tatgtgtttc cccgcttagt tttttcagca tgaattcagc
     2521 ctcagccaga tcttctggtt tacccatgat ctcttgattg agaacaacag tcgtgtcact
     2581 ggcaatcact aaatggtctt ctttttcatc ttgttcccag acagttcgtg ctttttcttc
     2641 agccattcta cgaacataat cgacgggctg ttcgtctgga aatggcgttt catcgatatc
     2701 agcaggttca atctcgaatt ctgaaacgat atgagccaat aactcttttc ttcttggtga
     2761 ttgggatgct aatacaatct tcatgttttc cctcttctct ttgacttaat gcgggtcttg
     2821 gattcgtttg aacatccttt ccatatccga attggtaaag tgaatcaata ctggacggcc
     2881 atggggacaa ttgaaaggat tttcgcatcg tgccagatcc ttcagcagca cacgtgcttg
     2941 tgcttcattc aaataatggt tggctttaat cgatcgtttg caactcatca tgattgctgt
     3001 tgcttcacgg aattttttta cactcacact tccagttgtc agcagcatat cgatcatctc
     3061 ccgaataatc gattcttctt ctccattggg ataccaagta ggatgggccc gtacaataaa
     3121 gctgttttga ccaaattctt ctaaataaat ccctgcatct tctagcaagt gtttttgctc
     3181 ttttaatttc aaagcatcac tgtttggata gtctaacaca aatggaacaa gcaattcttg
     3241 cagatcatca gatacttccc caattttttc ccggaaatat tcatacttaa tgcgctcttg
     3301 ggcagcatgc tgatcaatga tgtacaaccc atcgctactt tgggcaaaaa gatacgttcc
     3361 gtgcatctgt ccaaaatatt ccaactgagg aaaacgttgt actggttttt ccgtttctaa
     3421 tttcttgatg gcttgttcta atgttttgtc gtttccttga gtgaaatcaa attcaggatg
     3481 gagtgtcaat tcttcctcgt aaatcttttc atcctcttct acggatttat ctgcttgttc
     3541 ttcatttgtt ttctccatgt tatccacaat gggaaattct ttcttttcag atttttccac
     3601 tgttttttct acaaagttat caacattttc accaaactta tccaccgttt tcgctgtttt
     3661 ttctggataa gtggtgtttt cttcagaaaa accagaatag ttttcaacat tttccacaga
     3721 cttatcaaca attctgtcaa caaatgtgtt ctttagttct tctacgaaaa aggtacctgt
     3781 ctctcgatca aaattcaaac tacttggctt tttaggtttt tcttcttcaa aagcgtagga
     3841 caaatcgatt tcgatttgtt ctgttttagg ctgatcaggt acttttttct taaaccgtag
     3901 attatccgct gcacttggaa tcagattttc ttgagacaat gctttttgaa tggcgttact
     3961 aattagctct gttagttctt tttccttcga cagccggact tcctgcttcg tcggatgaac
     4021 atttacatct actaaaagcg ggtccatttt gatttccaat acagcaatag ggaaacgacc
     4081 gaccattaat tttgacccat aacctgcaac gattgcttta ttcaaggaaa agttcttgat
     4141 aaaacgtcca ttgatgatcg tcgataagta attcctactg gcacgagtca cctcaggcaa
     4201 tgaaacatag ccaaataact caaaatctag atcttttcct tcgattttca gcattttttt
     4261 tgcggtagat aatccgtaga ttcccgcaat tgtttgtttc agatcgccat ttccagcagt
     4321 tgccagcatt ttgtttccat catgtactaa acgaaaagca atcgatggat gactcaatgc
     4381 cagtcgattg acgatgtccc cgatgttcgc taattctgtg tgcagtgttt tcacgtattt
     4441 caagcgggct ggcgtattga aaaacagatt gctgacggta atcttcgttc cttgacgcag
     4501 tggtgctggt cgatgctcaa taacttctcc gcctttcagc gacagatagg tgccttgttt
     4561 ttcttcctga ttcgccgttt ccagagtcat ttcagaaaca gaggcaatac ttggcaaagc
     4621 ttcccctcga aagcccagcg ttcgtatgcg aaaaagatcg tcgcgattat ggatcttact
     4681 tgtcgcatgc cgcttgaagg cattctctac gtcgtctgcc aaaatgcctt ctccgttatc
     4741 aatcacttgg atcgtccgca gtcccgcttc ttcaactaag atatcaatct gagtactgcc
     4801 ggcgtcaatt gcattttcca ctaattcttt cacaacggaa gctggtcgct ccactacttc
     4861 tcccgcagcg atctgattag ctaaacgttc agataattct tggattttgg ccatcgatat
     4921 ccgccttctt cctacagttg tttttgtaat tcatataatg tgtttagtgc atccattggc
     4981 gtcatttcta gtaaatttat ttgcttcagt gattcaatga cttctgtttc attttcggat
     5041 aattcactga ataaagaaag ctgttggttt tcttctttta cacggtcttg tatatggtct
     5101 tgatttactt tttctgcagg tggttttatt tgctcctgtt tggctgtttt agttttctct
     5161 tctacttcag ctggaacagt atggtgtccg ttatcgccat tttctagcgt atgcaggata
     5221 tctgctgctc gttctagtag attggaaggc aatcctgcga tctttgccac atgtatgccg
     5281 tagcttttat ccgcaggtcc atccatcatt ttatgcaaga agacaacttc tccgtctttt
     5341 tcaacggctc caacatggac atttttaagc tgcggaagtt ctttctcaag taccgtcagt
     5401 tcatgataat gagtagaaaa gagtgttttt gcttgtacat gacgatggat atattcgatg
     5461 attgcttggg ccaacgccat tccgtcatag gttgctgttc ctcgccctag ttcgtcaaac
     5521 aaaatcaaac tgtttggcgt agcataacgc agggcttgat tggcttccat catctctacc
     5581 ataaaagtac tttgtccagc aattaagtcg tcacttgcac caattcgggt aaaaatccga
     5641 tcaaaaatag gtaaaactgc ttgttttgcc ggaacgaaac agcccatttg cgccatcaga
     5701 acagttaatg ccaactgtcg catatacgta cttttaccag acatattcgg tcctgtaatc
     5761 agcaagatca tttcgtcttc tgccatttct acgctgttag ggatatattc ctgatgtcct
     5821 aatacttttt ctactacagg atgtcgtccg tctttgatca gaagctgatg cttgtcactg
     5881 acaagttctg gccgaacata ctgatagcgt tcgctgatcg ttgcaaaact ttgtagcaca
     5941 tctactgcac tgattgcttt tgcaagtact tgtagcggct ggatagcctt tttgacttct
     6001 tctctcacag ctaaaaacaa ctgatattct aaatcaacag atttttcttc tgcttcgagg
     6061 atttgtgtct ccaattcttt tagttctggt gtgatgaaac gctctgcatt tgctaaagtt
     6121 tgttttcgtt catacttctc cagttctgca tttccaagat ttgcttttgt aatttctata
     6181 taataaccga aaacgcggtt aaatccgatt tttaagttct tgattcctgt ttcttgacgc
     6241 tcttttgctt caagttctgc caaccatttc tttccgtttc tcatagcaga acggtacata
     6301 tcgagctgct cattgaatcc atctttgatg atgtttcctt ccgttatttg taacggcgga
     6361 tcatcttgga tagcttggtc gatcaattgg acgagatggt ccatcggctg catttctgaa
     6421 agcagacttt gccattgacc ttgatcgatt ccttcaatca accctctgat cattggcact
     6481 tgttctaaag aagttttcag ctggatcaag tctcgcccat tgacattacc gaaagctact
     6541 cgacctgcta ggcgttccag atcatagact ttcgttaatg ctgactgcaa atcgatacgt
     6601 tcaaaatagc tgtctagcaa agaagcgacc atttcttgtc ttgctttaat ttgttttgcc
     6661 tggataagcg gacggtctag ccattgtttt agtaaacggc cacccattgc tgtttttgtt
     6721 tcatctaaca accatagaag agtgccgtgc tttttcccag tccgaataga ttggcttaat
     6781 tctaggttga attttgaata ataatccatt ttcaaaaaat gatctggttg gtattctact
     6841 gctttttgga tatgagctaa actccgcttt tgtgtcaccg ataaataagt caatagtttg
     6901 ccggttgctt ctttttccag cggatcgact agttcgcttg tcaaaaagct aaattccgcg
     6961 ttttcttcca tctcattttg ttgagaaaag actagcccta aacgcccgct tagctgttca
     7021 cgaagggtat cagtgatctc actgcctaat acgagttctt ttgtctgcaa tgcagaggct
     7081 tcatttaaaa cagcttcttc atcatgcaac cgagccgtct tcaattctcc cgtacttaaa
     7141 tcggcataag caaagccaaa ttcatttcct tgactaacta cagctgttaa aaaattgttg
     7201 tcttttgccg acaatccttt gctgtccatg actgtaccag gagtgactag ctggatcact
     7261 tcgcgcttga ccatcccttt agtcgtcttt ggatcttcta cctgctcgca aatcgctact
     7321 ttatagcctt tttcaatcaa ggcatcgata tatccttgtg ctgcatggtg aggtacaccg
     7381 cacataggaa taggttcttc tgcatttcga tttctgctcg tcaacgttaa ttccaaaagc
     7441 tgtgacgctt tgatcgcatc ttcgtaaaat agttcataaa agtctcctaa acgatagaac
     7501 aaaaaagcat cttggtattg ggccttgatg cttagatatt gttccatcat aggagtattt
     7561 ttagtttttt gaggcatgtt tttcctcctc tatttgtcca ttgatttctt cttgtatcat
     7621 agttgttaaa tgatgaagca gatcatctgc ttccaacagc tgttttctat aagctacaac
     7681 aagcggatgc tgatcaaatt cttgcatgaa ttgatccgct tgtttgattg cttctttttc
     7741 tgctgctggt ttgtcatagt gggcaaaatg aacagcatct ttttgcgctt gtttgatttt
     7801 ttctctcaac tcagctaaat actggttttg ctggatcttt tcttcaagtt ctttgtagcg
     7861 aatgatcgtt tcattctctc ttaaaagttt tgtcagtttc cttacttctt tttgaatttc
     7921 cggctcctgc tgcaaaaaac tgcctctctt tctactccat gaacggtcga tcatcgttga
     7981 tctgaatcag ctggatttct tttgcatgac ctgtcgtatc atccaattcc aagatacagc
     8041 cgcttaaaat cgatcggcct tgttccacaa cttcaaaacg ttttggtaat gcagtcagaa
     8101 acttttcgat tacaggttct ctgcgcatac ccaaaatacc atcataggga cctgtcatcc
     8161 caacgtctgt cagatacgct gttcctcttg gcaaaattcg cgcatcattt gtctgaacgt
     8221 gtgtatgtgt accgaccact gctgaaactt tcccatctaa aaaccagccc attgcttgtt
     8281 tttcacttgt tgtttctcca tgaaaatcta caaaaatgat cggcgtacgt tttctggctt
     8341 cttctaccag ctcattcatt ttacgaaaag gatcatccaa gtccaccata aaagaacgag
     8401 cctgcatgtt gatcactgcc agttcgactt ggttcacttt gacaaagacc atcccttgtc
     8461 caggcgtccc ttccggaaaa tttgccggac gaaccatctt tttagcttca tttacaaact
     8521 caaagattcc gcggttgtcc cacgtatgat tccccatcgt cacgacatct acgccgtctt
     8581 gcaaaaattt tttatagatt ttttctgtga ttccacgtcc agaagcggca ttctcaccat
     8641 ttacaatcgt tacttgcgga cggtacttct tcttcaatct aggaagatat tcagtaatca
     8701 tctctcgtcc cattgaacct actacatcac caataaacaa tacccgcaca ctaaattcct
     8761 cttctcttt
//
LOCUS       DAZUMV010000115         8533 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000115 DAZUMV010000000
VERSION     DAZUMV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.116"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..859
                     /locus_tag="ACG8FK_002278"
     CDS             80..859
                     /locus_tag="ACG8FK_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097844.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            900..1844
                     /locus_tag="ACG8FK_002279"
     CDS             900..1844
                     /locus_tag="ACG8FK_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5097845.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1860..2639
                     /locus_tag="ACG8FK_002280"
     CDS             1860..2639
                     /locus_tag="ACG8FK_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS5097846.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            2652..3350
                     /locus_tag="ACG8FK_002281"
     CDS             2652..3350
                     /locus_tag="ACG8FK_002281"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS5097847.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3378..4142
                     /locus_tag="ACG8FK_002282"
     CDS             3378..4142
                     /locus_tag="ACG8FK_002282"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS5097848.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            4155..5972
                     /locus_tag="ACG8FK_002283"
                     /pseudo
     CDS             4155..5972
                     /locus_tag="ACG8FK_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            5986..7209
                     /locus_tag="ACG8FK_002284"
     CDS             5986..7209
                     /locus_tag="ACG8FK_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFS5097849.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRNLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            7223..7957
                     /locus_tag="ACG8FK_002285"
     CDS             7223..7957
                     /locus_tag="ACG8FK_002285"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5097850.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITSETSILYKDEDKLLHDKNNIDKTYIEKILPSKMYYNLKSISEFSCMNDLKIMC
                     KTIFVIFGKEYHADLEGEREFINQIK"
     gene            7977..>8533
                     /locus_tag="ACG8FK_002286"
     CDS             7977..>8533
                     /locus_tag="ACG8FK_002286"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5097851.1"
                     /translation="MIEEIKSSDKELINSIVDIHMRTFTNFFLTFMGKKFLELMYRSY
                     TEYNDSGILVAIQNDKPIGFLAYSGDMSGLYKYMIKKRIIPFGFYSCVAFIKKPNILM
                     RLFRAFLKPSETKRDENYVELASIGVNPKEKSIGVGSMLIDNLKSRIDFSKYEYIALE
                     TDALNNDVVNNFYKKNEFILERKYST"
BASE COUNT         3014 a         1442 c         1758 g         2319 t
ORIGIN      
        1 tttaaaataa aaagcgctca ttctgatgtg aatgaatcac atctttattt atctatcatc
       61 aatatttagg agtgaatcta tggaaaagcc gttcgtcaag ttattggcta ctgtggcggt
      121 aggcacaggc gctattgtga tttgcttgtt tggataccat ctcaataatc agcggcaaca
      181 tcatcaacgg atcaattacg cagaatctgc catcaccaat caaaaagaca ccgtgacctc
      241 tttatcaaaa gaagtagata agctgtacag tacaaaagaa aaaattttcc taaatcctga
      301 aataacagaa gaaacagttt caaatttgtc acataaacta tcttctatca aactttcagc
      361 agatgattat gacatcaagg aatcagaatt acctaaagaa gctgctgcga tccaagaaga
      421 aaagaaagca gtcttaacac agttagagga tgcagaatca aaactgaaga tccaaacggc
      481 tgtcaataaa ttatttacta aaaacgtatc aaactggcag caagctgtgg atgatgtcat
      541 catcaaagaa aagttggcaa gtgcagatgt cgcccatgta cgtgaaaata tgagcttttt
      601 taaggatagt gcttggaaaa cggtcgttat gcaatatcta ggattcgctg atacccaaat
      661 tgcacaagtc acgcaactag atcagctatt cgatacgatg ctgaaagacg gtcaagtaac
      721 agctactgct acttacgatc aatatttgac cgcactcagt ctcatcgaac agatccggaa
      781 tgaaaaaata agagccgcat acgcgtcaaa agcagaaaca gtcgctcagc aaatgggcta
      841 tagcaatacg agctattaat aagcgagaat tcaagcaagg ctagcaaacg gaggaaacta
      901 tggcaaaacg aagaagaaga tcaagaggga aaaagatatt tctaattatt ttagcaattc
      961 ttgtcgtatt agtattagca atcgtaggag taggagcaaa attatatcta gatctctcac
     1021 attctattga aaaaacttac gaatcagtgg aacgaaccca aggagaacga acaaaaccag
     1081 ttgatctgga caaacaagaa tctttctctg ttctattgat ggggattgat acgggagatt
     1141 taggtcgggt cgatcaagga agatcagata cgatgatggt tgccacagtc agtcctgaag
     1201 acaaacagac aacgattgtc agcattcctc gtgatacgta tgtcgacatc gttggacatg
     1261 acacaaaaga caagatcaac cacgcttacg cattcggcgg agctgccatg gcgatggata
     1321 ccgtacaaaa atatgtagac atcccaatcg atcactatgt ttcaatcaat atgaaaggtc
     1381 taaaagaact cgttgatgca gttggtggta tcgaagtcaa taatgacctc accttctcac
     1441 aagatggcta tgattttacg atcggtaaaa tcacattgaa cggggatcag gcattgtctt
     1501 attctcgtat gcgttatgaa gatccaaatg gtgactacgg cagacaagaa cgtcaaagaa
     1561 agatcgtaga aggtgtagcg aaaaaagtgt tgagtctgaa cggtgtaagc aactatcaat
     1621 ccgttttgaa tgctctagag tcgaatatga aaacagacat gagctttgat gaaatgaaaa
     1681 agattgcttt agattatcgg gatgcatttg gtacgatcaa gcaagatcag ctgcaaggag
     1741 aaggcttcat gcaagacaat atctcatacc aaaaagtcag cgatcaagaa cttgatcgtg
     1801 tgaaaaaaga attaaaagat caaatgaatc tagagaataa ataggaaagt ggagaaaaaa
     1861 tggaagagac aatcagcttg aaagaaattt ttgaaacatt acgcaaacac gtgataacaa
     1921 tcctcatctc aatgtttgct ggcttggcca tctcaggcat cgtgacgttt ttcatcttga
     1981 cgccaaaata cagctcacaa gcccaattga tcgtgacact gccgcaatca gatactgcaa
     2041 atgtcaatga tgtcaatacg aacctgcaaa tgatcaatac gtataaggat atgatcgtca
     2101 gtgatctagt actaaatgaa gtcaaagatc gtttagaaac agaagacaat gtaaaaatga
     2161 ctgctggaca aatcaaagat gccatctccg tcaatcagtc acaaaattca caaatgttct
     2221 cgatccaagc aacaagcaca aatgcagtga cggctcaaca aatcgctaat acaacgtcac
     2281 aagtattcca agagaacgcc aaagacgtga tgaatgtcga taaaatctcg atcatctcag
     2341 gtgcagttgc caatacgaca cctgtttcgc caaataataa attgaatcta gtgattggct
     2401 tagtgttagg aatgatggta ggcgtaggtc tagctttctt atttgaatta ctcgaccgga
     2461 cagtaaaaga tgaaaaatat gtgacggata cattgggctt cccgatctta gggactgtac
     2521 cagaaatgtc agcgaaagaa ttgaatgctg gtatccgtaa aaaaggacca gctccgacac
     2581 aaccaaaagc tacaaacgat aaaacagcgc catcacgcag aagccgctca agagtctagg
     2641 aggaaagaaa aatggcacga acacagaaac aaaaagcagc ccaaactcat gcagtcagtt
     2701 tggtcacact cacaaacccc tcttcaccaa tcgctgaaca atatcgtacg attcggacga
     2761 atatccagtt tgcctccgca gctggtcagc aaattaaaac gattgttgtg acttcatcag
     2821 gacctggaga agggaaatcg accactgcag caaacattgc cgttgttttt gccaaatctg
     2881 gtcaacgggt gctgctagtc gatgcagact tacgtaagcc cgtcatctat aaaacgttca
     2941 agttgaacaa cgcaagcgga ttaagtaccg ttcttagttc atcaggaagt gtagcagatg
     3001 agatccaacg aacaccagtc gagaatttgt cgattctgcc aagtggacct aaaccaccaa
     3061 atccatcaga actactaagt tcgccaagaa tggatcagat tttagcagaa gcaagacaac
     3121 tatttgatgt agtgatcttc gacatgccgc cagtagttgc cgtaacagat gcacagatca
     3181 tgtcttccaa aacggatggc accttattgg tcgtacgtga aaatatgtca cggaaagaat
     3241 cattgactaa agcaaaagaa ttgctggata tggtacaagc gcgagtcctt ggtgttgtat
     3301 acaatggggc agaacacagc aaagatgctg gctattacta cgggaattaa gaaataaaaa
     3361 gagaggagac aagagaaatg atcgatctac attgccatat cctgccagga atcgatgacg
     3421 gagcagaaaa catagatgcc agtatcgcca tggctgaaaa agcgattcaa caagggatca
     3481 cacatatttt atgcacaccc catcataata atggaaaata cagcaatgaa aaatcacaag
     3541 tcatttctct agtcgcttcg ttacaagcag aattagagaa aagacaattg cctttgacgt
     3601 tattggaagg acaagaagta cgtattaccg gaacactgat cgaagacatc gaccgagatg
     3661 aaattttatt tacagattta gatgatacat atcttctaat cgaatttccg acactggaag
     3721 tgccgttata tgcagaacag ctcttttttg agttgttaca actaggaaag acaccagtga
     3781 tcgtccatcc agaacgcaac gcgcattttc gtaaagaccc gaatcatatg ctgccattcc
     3841 tagatatggg atgcttggcc caactcaccg caccaagtta tgtcggtgta tttggaaaag
     3901 acatccagaa aacagccaaa atgatggtca aacataatct tgtgcagatg gctgcttctg
     3961 atgcccatgg ggtctctaag cgcacctttt acttaaaaga gtgctataag cagattgcca
     4021 aggactttgg taaggaaaaa gtagtacaga tgaaacaagt cgcaaaggat ctgatcaacg
     4081 gggatgaaat cagttatcca gtctatgaag agataaaaaa gaagaagttt ggtttatttt
     4141 agggggagtg taaggtgttt gtattaacaa gaagaagaaa aattgccaca ttggttgttg
     4201 tggacagttt gttattgatc gctgcaaata ttgcagcagc taaattcatg aagccgtttg
     4261 tagctatacc aatggacctt attttaatct ctaccggttt gtcgattggt ttttatttat
     4321 tttatggtag tttattcaaa gttttcactc ggatcaatcg ctatacaaat ttaagagaga
     4381 tcattgcaat ttttggttca ctatctgcaa gtgacgtctc aagtatcctc atactgctgt
     4441 tcattaatcg gcaatacagt ttacggttag ttatctttgc ttatctgctt tcgctcttat
     4501 tgatcatcgg tagccgattg atctggcgta tctacatcga aacaaaaaat atgagatatg
     4561 tatcagcgga ttctgctaaa aatacattga tcgttggtgc tggcgaaggc gggcgtattt
     4621 tatacaacag ctttctggga tccaaaacgg ctcaagatat ccaagttgtc ggatttgtag
     4681 atgatgatcc gaataaaaga aatacgtatc tttcaggaaa aaaagtatta ggtgcattaa
     4741 aagatattcc ggaattgatt gaaaaatatg acattcaaat ggtaacgatc gctatccctt
     4801 ccttatcaag aaaaaaatta cgaagaatct tcgagttagt cgaatccgct cacgtgaaag
     4861 tcaacacgat gccttctata gaagaacttg cttcaggaaa aattagcgta tcaaaattaa
     4921 aaacaattga cgtagtggat ttactgggaa gagatgaggt aaagctggat attgaatcca
     4981 tcaaagatca gatcacagac aaagtcattc ttgtcaccgg cgcaggagga tcgatcggtt
     5041 cagaaatctg tcggcaaatc attcaattca atcctgcaaa gctattgctt ttaggacatg
     5101 gggagaactc gatctacttg attgaccgtg agttacgcac gcatcatcaa aattgtccta
     5161 cagaaatcgt accgatcatc gcagatatcc aagatcgcga gaaaatcaat gagatcatgg
     5221 aacaatatca tccagatatc gtctaccatg cagctgctca taaacatgta ccactgatgg
     5281 aatataatcc aaaagaagca gtcaaaaaca atattttcgg tacaaaaaat gtagcagaag
     5341 cagctaaagc agcaaaagtg aaaaacttcg tcatggtatc gacagataaa gctaacaatc
     5401 ctccaaatgt catgggttca acaaaacgaa tcgctgaaat gatcgtgact ggattgaatg
     5461 aagaaggctg tacgaaattc tcagctgtca gatttgggaa tgtccttggt tctcgcggta
     5521 gtgtgattcc agtattcaga gaacaaattg ctaaaggtgg tccaatcact gtaacagact
     5581 ttcgtatgac ccgatacttc atgacgattc ctgaagctag tcgtttagtt attcaatctg
     5641 gcgcattagc aaaaggtggc gaaattttta ttctagatat gagtgaacca gtgaagattg
     5701 tagacctagc caaaaacatg atccgattga gcgggtatag cgaagacgaa atcgaaatca
     5761 ttgaaacagg tatccgtcca ggggaaaaac tatatgaaga actactgttg gataaggaaa
     5821 gaaatgatga agcagtttat tagaaaatct ttgtaggaaa catcaaaggt tactcaatac
     5881 aaaaagtgat ggattttgtc aagagcctgc cgcaagacga tgaacaattg gctaaagata
     5941 tcgtcacttt tgcaaatgct tcgaataaat aggggggaaa tataaatgaa taaattagaa
     6001 aaacgtcaga taccattttc accgccagat attactgatt tagaaataaa aaacgttgta
     6061 gaagtattaa aatctggatg gataactacg ggaccaaaga caaaaaaatt agaaaaaaaa
     6121 attacaaagt ggttaggaac ttctaaagta gtttgtttaa attcacaaac tgctgctgca
     6181 gaaacagggt tacgtttgct gggaatagga gaaaatgatg aggttatcgt cccagcttat
     6241 acttattctg ccagtgcatc ggttgtatgc cacataggcg cgaacctagt attaatagac
     6301 tgtcaacaag attctttgga gatggattac gatgcattag aagcagcaat tacggagaag
     6361 acaaaagtga ttattcctgt tgatcttggt ggtatacctt gtgactacga ccgtattttt
     6421 gagattgtgg agaaaaaaag aaatttattt caacctagta atgagattca gaaagcttat
     6481 ggtcgtataa ttgttatggc agattctgct catgcatttg gagctaattg gcatgatcag
     6541 atggttggta atgttgctga cttgacaaca ttttcatttc atgctgttaa gaattttaca
     6601 actgcagaag gtggagcttt agtatggaaa gagcacgaca aactagataa tgaggcaatg
     6661 tatcatcagt gccagctgct ttctttgcat ggacagtcaa aagatgcact tactaaaaca
     6721 cagttgggtg catgggaata tgatattgtg ggtccatggt ataaatgtaa tatgactgat
     6781 gttactgcat caattgggtt ggcacaattt gaacgctatc ctaatattct gaaaagaaga
     6841 aagaatataa ttaagaggta tgatgaagcc tttcaacaac taggaattga agttcttatg
     6901 cattatacgg atgattatag ttcttcaggc catttatata ttactagagt acctaacatt
     6961 agttctgagc aaagaaatga aataattata aaaatggcag aacaagggat tgcttgcaac
     7021 gttcactata aaccacttcc aatgtttaca gcatataaaa atatggggtt tgatattaat
     7081 aactatccta atgcctatgc tcattttgta aatgagatta cattaccatt gcacacgaaa
     7141 cttacagatg aagaagtaga atacgtaatt aataactata gtaagattgt aaaggatttg
     7201 gttggatgat aggagaggca aaatgagaaa gtgggaagat ttgccaaaag aaatgcagaa
     7261 tgatgaaata cggtattatt atgatatcct cagtaaaaaa aaattcagct tagtggtaaa
     7321 aagagtattt gatattttag tgtctgcaac tttgttactg acactttcat ggttattttt
     7381 tattcttgca atagtgataa aaattgattc tcctggacct gtattttata gacaagaacg
     7441 tattactcgt tatggaaaaa aatttaaaat ttataagttt cgtactatgg taataaatgc
     7501 agataaagat ggcactcttg ttactgtgaa aaatgatgat cgtattacaa gagtaggaaa
     7561 ggtaatccgt aaatatagat tagatgaaat tagtcagcta attaacgttt tggtcggaga
     7621 tatgtctttt gtaggaacca gaccagaagt tggaaaatat gtaaagtcgt attcaccatt
     7681 aatgttagcc acattattat taccagccgg gattacatca gaaactagta ttttatacaa
     7741 agatgaagat aaattgttgc atgataaaaa taacatcgac aaaacttata tagaaaaaat
     7801 tcttcccagt aaaatgtatt acaatttgaa aagtatcagt gaatttagtt gtatgaatga
     7861 tttgaaaata atgtgtaaaa ctatttttgt tatatttggt aaagaatatc acgccgactt
     7921 agaaggtgaa cgagagttta ttaatcagat aaaatgaatg gaggataaga gttttggtga
     7981 ttgaagagat aaaaagttcg gataaagagt taattaattc gattgtcgat attcatatga
     8041 gaacatttac aaattttttt ttgactttca tgggaaaaaa gtttttggaa ttgatgtatc
     8101 gttcttacac agaatataat gattctggta tattggttgc tattcaaaat gataagccaa
     8161 taggatttct tgcatattct ggagatatga gtggtttata taagtatatg ataaagaaaa
     8221 gaataatacc gtttggattt tatagttgtg tagcctttat aaagaaaccc aatatcctta
     8281 tgaggctttt tagagctttt ctaaaaccaa gcgaaacaaa acgtgatgaa aattatgtgg
     8341 aattagcatc cattggagtt aatccgaaag agaaatcgat aggagtaggt tccatgctta
     8401 tagataactt aaagagtaga attgattttt caaaatatga atatattgca ctagaaacag
     8461 atgcattaaa taatgatgtt gtgaataatt tttataaaaa gaatgagttc attttagaac
     8521 gtaaatattc tac
//
LOCUS       DAZUMV010000116         8528 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000116 DAZUMV010000000
VERSION     DAZUMV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.117"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..891
                     /locus_tag="ACG8FK_002287"
     CDS             64..891
                     /locus_tag="ACG8FK_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5097852.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(1164..1934)
                     /locus_tag="ACG8FK_002288"
     CDS             complement(1164..1934)
                     /locus_tag="ACG8FK_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5097853.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            2152..3219
                     /gene="ulaG"
                     /locus_tag="ACG8FK_002289"
     CDS             2152..3219
                     /gene="ulaG"
                     /locus_tag="ACG8FK_002289"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS5097854.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            3237..3698
                     /locus_tag="ACG8FK_002290"
     CDS             3237..3698
                     /locus_tag="ACG8FK_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5097855.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            3718..5202
                     /locus_tag="ACG8FK_002291"
     CDS             3718..5202
                     /locus_tag="ACG8FK_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5097856.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            5245..5544
                     /locus_tag="ACG8FK_002292"
     CDS             5245..5544
                     /locus_tag="ACG8FK_002292"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5097857.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            5559..6197
                     /locus_tag="ACG8FK_002293"
     CDS             5559..6197
                     /locus_tag="ACG8FK_002293"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS5097858.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            6202..7062
                     /locus_tag="ACG8FK_002294"
     CDS             6202..7062
                     /locus_tag="ACG8FK_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS5097859.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            7052..7762
                     /locus_tag="ACG8FK_002295"
     CDS             7052..7762
                     /locus_tag="ACG8FK_002295"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5097860.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            7994..8428
                     /locus_tag="ACG8FK_002296"
     CDS             7994..8428
                     /locus_tag="ACG8FK_002296"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5097861.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT         2814 a         1334 c         1825 g         2555 t
ORIGIN      
        1 ttagaacttt tttttgtgta tgatataaag gagaaataat tttttaaagg gaaggaaatt
       61 gttttgatta aagtagatgg agcattacta aaagaatacc gaaagaaaaa ggggttaaca
      121 caagtacaat tagcagaagg catttgtgaa caagcaactg tcagcaatat cgaaaacaag
      181 aatatgtcgc caggcatcaa tatcttaagt gcgctttgca aaaagctgaa tgtcgagatc
      241 tccgatatat tagtagaaac agaagaagac aaaatcagga aaaaaataaa tgagattcaa
      301 tcattgaatt ctgtatcaaa ataccaagaa tcttataaaa ttttacaaac gatcgatcag
      361 gaaaaagtaa gtaattctga tcttttaaat caaattttat tttatcaagg actgcttgat
      421 catctttatg cgaaaaaacc agatgcagca ttattttatt ttaatcaagt gttggaacaa
      481 acgaccgaaa cagatatcca tcacttacaa gcagctgcaa acgtggcaat gatctacttg
      541 tcaaaaggag aattagattt tgcgaaagtt tatgtggagc gtacacttaa aatattgagt
      601 gagactgatt ttgataatct gatggtctgc attgtatatt atgatatcgc cacctattac
      661 cgtaaaatcg aggattatga taaagcaatc caattatgtg agaagggtat tgagtataat
      721 aagaaacata aatcgactta tgcattggaa tacttgcttt atgaaattgc ttcttgccat
      781 aagcaattag gagaagatga ttatttagaa agatatatgg acgcaaaaaa aatagcgcgt
      841 ttcaacggaa atgattacgc ggtaaaggtg attgaaaacg acctgaaata aagtggttgt
      901 gaaatcagtg ttctgatttg aataccgttt attcgaaaaa aggaggtgtg acttttgtca
      961 tacctccttt tttcgatatt gtttgattcc tatttaggca acatataacg gttataaaag
     1021 aagcaataga ttatgatggt atcgattgga tggatacatc ttgggattcc tttatcaatt
     1081 tgagcgtagc gtgtttttca tttatcgata aatttgttat tcattttttt gataatccta
     1141 tttacatatt tacgtcctgg ttcttaaaat gaatctactt gaatggtttt tatccccgta
     1201 tttctgatct cctctaatac tttttcagaa caaaatttat cagtgatcaa ataatcaaag
     1261 actgaaatat cagcaatctt gaactttgat gtcagtccga tttttcgatg atcagcgact
     1321 aaaatcttac atttgattgt cttgttgacc atcatttcgt tgatcgttga ttcatttaat
     1381 atctttgttg tgaccccatg tactacatcg actccgctac agccaatgat acaaatgtct
     1441 gcattgactg aagaaatcgt atttaatgcg atgtcgccca ctagtgcttc ttttggaaag
     1501 cgcatctctc caccagttaa aatataacta caattatctt tgtgcggaga gtaattgatt
     1561 ttcacgttgt ttgtaatgac tgtcaaatgg ttagattgaa gaaaatccag tgttttcaac
     1621 gcagtcgagc tagaattgat aaacagcgta tcataatcgt taatgtattt actagcttct
     1681 ctggcaatcg cttgtttaat cctttcagga ccagattgat caaaatcagt ctggtttttg
     1741 taattataag aacaataacc gtaattgcgt atgatctcgt ttttttcttc taataaattt
     1801 aaatctcttc gtatcgtact aatagataca tcaagggatt cagctaattc gaaagttgat
     1861 agttgttcag tctgctgaag cagttccaaa atttcttttt gacgtttttc aatattgaca
     1921 agtgagtttt tcatgacttt tgcctcctga gggacatctt gatccatgga tttatttgac
     1981 tagttgttca tttaaacatt catatctgtc tttattatac tgaaacaatc attgattact
     2041 gaaagaaaat catataaaaa acaaacggtc atataaaagg gttttcatgg atgctgatac
     2101 acattaatat ttacttgtaa gttggaacaa cgatatgggg ggagcaagca aatgagtaaa
     2161 actgtcaatg aagtaacaaa agaaaagtgg ttagtagaaa catttcctga gtggggcaca
     2221 tacttgaatg aagaaatcga aacagaagta gtggatcctg gaacttttgc tatgtggtgg
     2281 ctgggctgta cgggaatttg gctgaaaagt catgaaggaa cgaatatttt atgtgatcta
     2341 tggtgtggga cgggaaaacg tagtcatggg aatggcatga tgaaaaaagg ccatcagatg
     2401 atgcgtatga gtggcgttca aaatatgcag cctaatttac gtactcagcc tttcgtaatc
     2461 gatccttttg cgataaaaaa tgtagacgct ttaatcgtta cacatatcca ttccgatcat
     2521 ctagatatcc atacagccgc agcagttcat caaaattgtc ctgaagcaac attcattggg
     2581 ccaaaagaag tggttcgcac gtggcaaaaa tggggaattc ctgaagaaaa aacgattaca
     2641 gtaaagccag gtgatgcagt caaaatcaaa gacatcaatg tagtagcttt agaagcgttt
     2701 gatcggacag cgttggtcac ttgtgaagat ccagaagagg tattaaaagg aaaaatgcct
     2761 caggagatgg atcaaattgc agtaaactat ttattcgaaa catcaggagg gaatctttat
     2821 catgcaggag attcacatta ctccaattta tttgccaaac atggtaatga acacaaaatc
     2881 gatgtctgtt taggggccta tggtgaaaac ccacgaggga taacagacaa agttacttct
     2941 gttgatatgc tgcgcatggc ggagtcattg aatgcaaaag tagtgattcc tgtccactac
     3001 gatatttggt ctaattttat ggctgatcca aaagagatca ctgaaatttg gaaattcaaa
     3061 aaggatcgcc tgaagtatgg atttaaacct tatatctggc aagtaggcgg gaaattcatc
     3121 tatccgacaa acaaagatga tcttgagttt aatttttatc gaggatttga agatgtcttc
     3181 tcgattgaaa atgatacacc gtttccgtcg tttttataag aaagaagggg agtttcttgc
     3241 tgaaatactt ttttgaccat caattgattc attacccaga taaagaaatc acggattggc
     3301 gtgaagctat cgccgaaagc tgtcttgtat tgatcgataa ggaaattatt gatgaaacgt
     3361 atgtggagga gatcatccat tgtgtagaaa aatacggtcc ttatattgtg atcgcacctc
     3421 aagtagcaat gccgcattct tcagaaaaaa gtgatggtgt ttttggaaca gctatttcat
     3481 ttaccaagtt gaaacagcca atccgttttg aaagtgaagg ggagaatgag gagaaaacgg
     3541 ctagtttgtt ttttacactc gcagcaaaag atccagaaga acatctagaa aatatccaaa
     3601 aattatccga attattaatg acagatggat tggttgagcg tctcatctct acgaactcca
     3661 tcgatgatta ccaagttgtg atggcagaat ataattaatt gatagaggag aaaaaagatg
     3721 ggggaatttt tgttaagcat ttggacgtat tttgcaacaa atatcttgac gcagccagca
     3781 tacctgattg gttttatcgt gttattaggc tatattttgc tgaaacgccc gcttcatgaa
     3841 tgcatcgctg gattcttaaa ggcaacggtc ggttacttta ttttgactgt tggctctggt
     3901 ggtttggtca ataattttcg gccaatcttg gttggactaa aggaacggtt caatttggat
     3961 gccatggtta cagatcctta ttttggacaa aatgctgtgg atgctggatt aatggagaca
     4021 tttggccgta cctttggaga tgtgatgatt ttattactga ttgcatttgt tttaaatatc
     4081 ttattagtcc gttttcagaa atatacgaaa ctgcgttccg ttttcactac cggaaacgtt
     4141 caggttcaac aagcagctac cgctttttgg attttactgt tttgctttcc tgatctaggt
     4201 aggatacaag tattgatttt tatgggactg attttaggtt gttattgggc agtagcttct
     4261 aacttaacag taggaatcac gcaggaattg acagaaggtg ccggatttgc agtggcacat
     4321 cagcaaatgt tcggaatctt tatatttgca aaattagctg agtggatgaa aaagcgggat
     4381 gaaaagaaaa atagatcaat caagcaagac aaaaaaattg aagatatcaa attaccagga
     4441 ttcttgtcga ttttcaatga gaatatggtt gctacttcgt tactcatgct atttttcttt
     4501 gggatcattc tgattgtact aggcaaagat tatttgattc aagcgcaatt catgcaagaa
     4561 gggcagagtt tcttattcta cattatgacg acttctttaa attttgcggt atatctggcg
     4621 atcttgcaat taggtgttcg gacattcgtc gatgaactaa ctcaatcttt ccaaggaatc
     4681 tcaaacacga ttttaccagg agctgttcca ggaattgacg tagctgctac ttttggcttt
     4741 ggttcgccaa atgcggtaac gattgggttc ttgtttggtg ctttgggaca gtttttgatg
     4801 attggtttac tgattttatt gaaatcacca acgatcgtca tagccggttt cattccgttg
     4861 ttctttgata atgccgttat agcagtttat gccaataacc gaggtggatt caaagccgct
     4921 tgcctgttcc cgtttctttc aggaatcata caagtaggcg gttcagcatt attcgctaca
     4981 tggattggga tgtcaacata cggtggttat atcgggatgt tcgactgggc aacagcttgg
     5041 ccattgttta cagtaatcat gaaagtccta ggttatgctg gaattgccgt agttgtcatt
     5101 ggtttattgg ctattccgca gttacaatat cggaaaaatc ctgatggtta ttttttgatt
     5161 gtagaagact atgacacata tgcagaaaag cagagagtat aaaagaaaat ctgattagac
     5221 aacactcata atggagggat taagatgaga attttagtat cgtgtgcaaa tggatcagga
     5281 acaagcttga tgatgatgag aagtgtggaa aaagcaatga aagaactaaa cgtaccaatt
     5341 accaaaatcc atcattgtgc catttcagag ggaaaaagtt cagctggcca atacgatgta
     5401 gtttttacgc cagtcaattt tttgaatatg tttactcagg cagagaaaaa aggagttaca
     5461 gttatcggta tcaaaaatgt gatgtcagtc aaagaaatca gtgaaaaatt tgctgaaaca
     5521 aagtggtacc aaaaaaacaa gtaaaggaag gaactaagat gagcaggccg aatttgcaaa
     5581 ttgcgttaga tcacaatgac ttagaacatg cattaggaga tgttatgaaa gttggtgatg
     5641 tagtagatat tattgaagtg ggcacaattc tttgcttaca ggaaggacaa aaagcgattc
     5701 gctgtattcg cagtatgttc ccagataaaa aactagtcgc tgatacgaaa tgtgcagacg
     5761 ctggaggaac agtggcgagt aatgttgcaa aagcaggagc agactggatg acagtcattt
     5821 gttgtgcaac gattccaact atggaggcag cacaaaaaga aataggtgaa ttgcaagtag
     5881 aactctacgg caattggact tttgaacaag ccatggattg gcataacatc ggtatccggc
     5941 aggtaatcta ccatcaaagc cgtgatgcgc tgttatctgg tgaaacttgg ggtgaaaaag
     6001 atttatcgaa gatcaaaaaa ctgattgaac taggctttaa tgtttctgta actggtggat
     6061 taaatccaca cacattacac ttatttgaag ggatcgatgt gtataccttt atcactggtc
     6121 gtggtattac agcagcaaat gatccgatga aggccgcaca aaacttcaaa gatgagatca
     6181 ttcgtatctg ggggtaagga aatggctcaa attgggattt atgaaaaagc gttacccaaa
     6241 gatatttcat ggaaagaacg tttttccctt gtaaaagaaa tggacttcga ttttatcgag
     6301 atgtcgattg atgagacgga tgagcggtta gcacgattag attggtctga agaaaagata
     6361 gctgagctga gagaagagat gttcagctcc ggtgtacgta tccattcgat ttgtttaagt
     6421 gcgcatcggc gatttccgtt tggttctgct gatccagaaa agaaaaaagc agcgaaacaa
     6481 ctaatgaaaa aagcaatcca tttggcgcat aatctaagcg tcaaagtaat ccaaatagct
     6541 ggatacgatg tttattatga agagaaatca atgaattcca gagaagactt tatcgaagga
     6601 ataaaagaaa gcgtaaagga agcttctaaa tatggcatca ttctttccgt ggaaatcatg
     6661 gatgatccat ttatgaactc catttcaaaa tttttagaaa tcaaagaaca gattcattca
     6721 ccttttctac aagtatatcc agacctaggc aatttgtccg catggccgga aaataatccg
     6781 gctcgagaac ttgagaaagg gatcgattat atcactgcta tccacttaaa agatacttac
     6841 cctgtaacaa aagaatattc aggccagttt cgtgatgttc cttttggtag cggttgtgtt
     6901 gattttcttg gattattaag acatttgaaa cgtctagatt atgatggaac atttctgatt
     6961 gaaatgtgga gtgaaaaaag ccaagacttt cgaagagaaa tccagcaggc aaaagactac
     7021 ttaagttcta aattgaagga ggctggctat gatgtcttgt gaagaaaaaa ttgcagcaat
     7081 gaagcaacgt gtccttgaag caaatctggc attgccgcga tacggattag tcaaattgac
     7141 ttggggcaat gtgagtgaag tggaccgtga gctaggagtc atcgtcatta agccaagcgg
     7201 tgtcagatat gaatgcatgc aagcagatca aatggtggta acagatttgt cgggaaatat
     7261 aatagaagaa gatagcttaa aaccatcttc agatctcccc acacatgttg ttttgtatca
     7321 aacttttgaa gatataactg ctattactca tactcattcc acgcactccg tcatgtgggc
     7381 gcaagcagga cgcgatttgc cagcttatgg cacgactcat gctgatgctt tttatggtaa
     7441 ggttccttgt acacgccaat taacgaaaga agaagtaaga gaagcttacg aagttcatac
     7501 agggaatgtg attgtcgaga cgtttaagga aagaaaatta gatccaaacg aagtccctgg
     7561 agtacttgtc tatggacatg gaccgttcac atggggggat tcacctatga aagcagtaga
     7621 aaacagtttg atcttagatg aaatttgtct gatggccaaa gaaaacgagc tgatcaaccc
     7681 caatatatgt gaaatcccgc aatatttact agataaacat tattttcgca aacatggaaa
     7741 gtatgcatac tacgggcaat agagatgcta gggtaagggt agaaaattaa aagataacac
     7801 ttgatagaga gaaacagagg ttgtgaagaa aatttatcac aaccttttta tttatcaaaa
     7861 agtatgcatt ctaaaaggaa agaggaattt taaggaatcg agagaatctt tacaggagag
     7921 ttatatgtga tagattaaat agtcaaagaa ctgtattatt agagggagaa ttgtataaga
     7981 aaggaagaaa aaaatgaata ttgattccct tatgatcgat gagatttctg aattatataa
     8041 gagcgttggt tggacgaatt acacaaaaga cactgcaagg ttagctagag catttgaaca
     8101 atctgaatct ctgataaagc gtaatgggga agggaagatt atcggaatcg ttcgttggat
     8161 cactgatttt gcgacgatcg catttattca agatatcctc attcatccga ggtatcagcg
     8221 ccaaggaata ggcaaagtgc ttttgaatga agctttggag aagatcacta gttatggacc
     8281 agtccaaatc gaattattga cagatgatac tgagaaaaca aaaaaatttt atgaatcagt
     8341 aggatttgtt caagtaaagg aaatgggtgg tatttcttat gtcatagata ctagaagaaa
     8401 aaaggtgctt tttggtaatg acaaataaca tataataacg tacaaaagaa aaattctagc
     8461 ttagttcttt agaaaaacaa gaggatagac ttagtatcac tcaatctatc ctccaatgct
     8521 cagctatt
//
LOCUS       DAZUMV010000117         8317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000117 DAZUMV010000000
VERSION     DAZUMV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.118"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..679)
                     /locus_tag="ACG8FK_002297"
     CDS             complement(68..679)
                     /locus_tag="ACG8FK_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5097862.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(802..927)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1009..1725)
                     /locus_tag="ACG8FK_002298"
     CDS             complement(1009..1725)
                     /locus_tag="ACG8FK_002298"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5097863.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1731..2300)
                     /gene="scpB"
                     /locus_tag="ACG8FK_002299"
     CDS             complement(1731..2300)
                     /gene="scpB"
                     /locus_tag="ACG8FK_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS5097864.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2284..3054)
                     /locus_tag="ACG8FK_002300"
     CDS             complement(2284..3054)
                     /locus_tag="ACG8FK_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS5097865.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3051..3422)
                     /locus_tag="ACG8FK_002301"
     CDS             complement(3051..3422)
                     /locus_tag="ACG8FK_002301"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5097866.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3441..4328)
                     /gene="xerD"
                     /locus_tag="ACG8FK_002302"
     CDS             complement(3441..4328)
                     /gene="xerD"
                     /locus_tag="ACG8FK_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS5097867.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4424..4897)
                     /locus_tag="ACG8FK_002303"
     CDS             complement(4424..4897)
                     /locus_tag="ACG8FK_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5097868.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5002..5865)
                     /locus_tag="ACG8FK_002304"
     CDS             complement(5002..5865)
                     /locus_tag="ACG8FK_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS5097869.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6061..8160)
                     /locus_tag="ACG8FK_002305"
     CDS             complement(6061..8160)
                     /locus_tag="ACG8FK_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS5097870.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT         2109 a         1753 c         1423 g         3032 t
ORIGIN      
        1 attattgcat gtcttgtatt tttttccaaa aaaaaaagac caagaaattt cttgatcttt
       61 ttgatattta tttgactgct gtatgacgtt gttcgaactg atgttgtttt cgacttaacc
      121 atggaagaag ttgcgcatgc agcactaaaa agacagcact cacgatgaat cctttgatca
      181 agttgaaagg aacgatccca atcagtacat agtttgctaa agacatgccc agcatctgtg
      241 ttgcgttcag ccctaaaaac attaagtaaa gaggcgtaat aacgaaataa tttgcgatac
      301 tcataaaaat agttagagcg atggttcctg taatgacacc taaaaatttg tttcttcctt
      361 tatgctgttt ctttacacta aagaaataat aaatcgggaa tgtaaagatc gttgttgata
      421 agaaactagc agtgtctcct accaaattat ccggagaaaa acctgtagtc agcagatgta
      481 atactgaacg caagaacgct gttgcaactc cggccgctgg accgaataag aacatgctga
      541 tcaataccga gatatcacta aaatcgatct tcaaaaaact aaaagccggc atgatcggaa
      601 aagcaaaata ctgtaaaact aatgcgatcg cagcaaacat cgctactgca accattttct
      661 gtacacgggt gttcttcatt tgaaatcctc ctgttaggac tcctcttcag cgaacttttt
      721 gacgtttctc tccaaatgaa aaaactctat tttccaggga aaatagagtt agagttattt
      781 gatcaagcgt cgtcaaataa gctctatctt ctttatccag actatactgt cggtatcgga
      841 atttcaccga ttcagctatt tttttgcaaa ataggtcgtg gactataacc accggtcggg
      901 aatttcaccc tgcccctgaa gacgaacctt atataatttt tgtttacata ttcattatac
      961 atatctattc aaaaaacgca acatctaaca cttataaaaa gtaagtgttc attttgagtg
     1021 gttcagcaaa ctgctgactt cttttttgtt cagttcacgg taatccccag gacgtaggcc
     1081 ttgtaaagtc agatctccgt atttttcacg ttttagtttt tgcacaggaa gaccaactgc
     1141 ttgaagcata tttttcactt gatgattacg tccttcgtgg atcgtcaatt ccacgatact
     1201 gtggtttgtc ttaggatcca ctgataaaat ttggaattta gctggagatg tttttcgtcc
     1261 ttcgatcttt attccttttg tcaaaggtgc aagcatcgat tttgtagcga tcccttttac
     1321 ctttgcaacg taaactttat ccacttcatg ttttggatgt gtcaggcgct gagcaaaatc
     1381 tccatcattt gtcagtagca agatccccga agtatcgtaa tccagtcgac ctacaggata
     1441 gatacgttct tttaccatcg gcaaaagatc cgtaacaact tttcttcctt tgtcatctgc
     1501 aacagcagaa ataactcctt taggtttgta caataaatag taaccgtatt cttcttgata
     1561 aattgggact tcatccactt ccacagtgtc atgtttccca acttttatcc ctaattccct
     1621 tacaacttgt ccgtttactt ttacacgtcc tgcaaggatg aattcttctg cttttcgacg
     1681 agaagtgatg ccagcgtggg caattacttt ctgcaatctc tccattacaa ttattctcct
     1741 ttattcattc catcaaagaa tagatctggt gacacgtctt cttcaagtga aatttccatt
     1801 tcttgtgtat caggcagttc ttctaatgat ttcaaaccaa aataatctaa gaagtagcca
     1861 gttgttccat ataatatcgg ccgacctggt ccttctactc tacctttttc ttcaatcagt
     1921 ttatgggcag ctagacgctg taccgctcct gaaacctgaa ctcctcgaat ctgctcgatt
     1981 tcagctcttg tgattggctg tttataagcg atgattgcta aggtttcgac ggcggcttga
     2041 gataatgtgt ttgatgtccc ttgcgcatat cgcttaagta agggagccag ctctttttta
     2101 gtcgttaata cataatggct ttccgtttca aaaatatgca aagctgagtg cgagttttga
     2161 gtgtaagatt cttttaattc tttcactaaa cttgtgactt ctgcttcgct gagatttaac
     2221 agtccgctta attcttcatt actgatccct tcctcgccaa caataaaaag aagggcttca
     2281 agttcactta atttcgtcat gttcggtatc tcctactgct ggataaagca taattgattc
     2341 atagttttct ttttgttgcg caatgatctg cccttttttc atcaattcta gcaaagccat
     2401 aaaagtagtg acaacttcac ttttagaagg tgactcaaaa aaagaatcga aagtacagcc
     2461 atgcgcattt ctttttatac gctcttcgat aaataccatt cgttcttcga tcgttgtgtc
     2521 atcgggcgtg atcgtttttt ccactttttg agaaaatttc ttacgttcta agattttctg
     2581 catggcacgg atcaaatcga tttttttgac ttgtcctcgc ttcaacggct tttcagcatc
     2641 cgctaaccca tcgatgttca taggttcttt tgtgtaataa agacttcgtt cctctgcttt
     2701 ttctgtcagt ctttcagctg catatttata tttacggtat tctaaaagct gagtgaccaa
     2761 ttcttctcga ggatcttctt cccataattc ttcatcgtct gtgacgaatt cttggacagg
     2821 aagcaatgtc ttgcttttga ttgccatcaa tgtagcagcc atcactaagt attctcctgc
     2881 aacagctagc tcgagtgttt tcattgtatg gatataattc atatattgct ccgtgatcgt
     2941 agcgatcggt atatcatata tatcgatttc catttgctga atcagatgaa gcagcagatc
     3001 gagaggaccc tcgaatacat ctaatttaat attgatctcc gtcaactcat tcactctttc
     3061 ttctttttct ttgctagcca ttttccaatg taaggctctg tgatttcagt tgcacggatg
     3121 gagacatccg ggtacttttc ctgtaattca tctaacattt gaaagcccat attctcaccg
     3181 cgataagacg gattcaaaga aatgtcatgg atgatcagct gatcgttcgt tgcccagtaa
     3241 ataccaataa tcccttgaat gttggttgaa ccttctggcg taaagaaaaa aagttcataa
     3301 tcgtcttcag attcgtacgt atcaatctcc ttcaacaaag aagttttacc tgtcagtttc
     3361 ttatggaaac tcaatagtcc catagcaatt ttacggtttt ctttccggta atgtgttaac
     3421 atcttctcac cttcctatga ctaagccctt ggaaaatgct gcttataaac atccgtcatt
     3481 cttttcttcg tgatatgtgt ataaatctga gtcgtagaga tatccgcgtg acctaacaat
     3541 tcctgtactg tccgcaaatc agctccattt tctagcagat gtgtggcaaa gctatgccgc
     3601 agcgtatgtg gtgttacttc tttgtaaatc ccagcctctc gtactaactg cttcagattt
     3661 ttccagattc cttgccgaga aagacctttc ccatgattat tgacaaatac atgcatttca
     3721 gatgcgtcct ttacaagtaa tggacgtgct tcgtctaagt agcgttccag ccattggatc
     3781 gcataatcac ctaaaggaat gatccgctct ttatctcctt ttccaactgt ctgtaccagt
     3841 ccaagagaaa gatgaagatc tcccaatttc aatccaatca attcactgac gcgcatccca
     3901 gtagcataca tgacttccaa gattgcacga tctcttatcc ctaacgtttt tgtcgtatca
     3961 ggtgcttcaa tcagcttttc tacttctgtc agagtcaatg tacttggtaa tttctgtgct
     4021 ttcttcggcg tatcgatatg ttgcatcgga tcatgatcag tgatacgctc ttgccgtaaa
     4081 aactgatgga atcttctgag acttgagatc atccgagtaa cagttgctgt cgcttttttt
     4141 tcttcatgca acttctccag aaaacggatg actaaaaagc gatcgacatc ctgccatgaa
     4201 gcaattttct gctcctgtag aaaatgcaag tactgttcca aatcacgttc atagctttgt
     4261 cgtgtattgg cggacaatcc ccgttcaata ttcaagtagt ggagataatc gttgatttgc
     4321 tctttcataa taatccccct catgcctctc tatcatatca gataccgctt cattctgata
     4381 gtctttttcc ataatctgag caaagaaaaa gccgtctaga cactcagacg gactggtttc
     4441 ttttttcttt ttcatagcat tcctgacaga ttccatggaa cgttagccga tgatctttta
     4501 ctaaaaaatg ataacgttct tctactactt gctccacttc aagtaaaaga tcctcttcta
     4561 cttcctcgat cgtgccgcag ttcaagcata gtaaatgatg atggaaatgt ttggcgcctt
     4621 cttttctcaa atcataacgg gctaatccat cattaaaact tactttatcg acaattttca
     4681 attctgtcaa gatttcaagg gttcgataaa cagtagcaag tccaatttcc ggactctttt
     4741 ttttcactaa aaagaagatt tcttctgcag ataaatgctc tttctcattt tccaaaagca
     4801 caagcagagt agcttctctt tgcggtgtca gcttaaaccc cgcttcatgt agctgctgtt
     4861 ttgttttttt catcgccgaa atagcattca tgaccattta ctcaacccca acaatttata
     4921 ataattacaa ataaaaaaag atataacact atgttaatta aaattattat aagctagtta
     4981 agaaaaacat gcaagggatt ctcaattaga ttttttgact agttttgtca caccgttttc
     5041 ttgtgtaatc aatccttgtt tcaaaagatt acctaatgcg cgcttaaatg ctcctttgct
     5101 gataccaaaa gctttcatga tttcttctgg atccgattta tctgtaaagt tgatttgctg
     5161 atctgcagaa cgttctaaaa tggttaaaat catttgtgca tcatctgata tagcttcata
     5221 tgctcttggc ttcaatgaaa gattcagtac accgtctggt cggacaccaa tcactcgacc
     5281 ttttatctgc tcgcctagac gaggttcatt atatcgttca gaaggatgga taaacccaag
     5341 atagaaatct tccgttaaaa cgtacgtacc tgttaatttc aagcggtatg ctgttcctat
     5401 cacatctttg tttttcattt cttctgttcc atatttgctt aaagatttga atattttttc
     5461 atctgctaaa ctgccccaaa tacggtcttt tgcatcaact ctaagggaaa tcatcaagcg
     5521 atcccctttt ttaggccata gctctgtcat tgtcggcaat tcatcaagag atacgacaaa
     5581 gtctttatct ggaagaccga tatcgacaaa cgcacctaaa tcttttcgtg tagcagtaac
     5641 gattccaaaa gcgtaatgac cgatacggct tttaggaatt tgtgtagtga tccgattttc
     5701 atgtttttga ttttggtaac cgaatccttc aactgcttca ccgattttgt gttcaccttc
     5761 tgatttgctt aagccaaagg tttgtccgtt tttttggaca aagtagtttt tttcattttc
     5821 atcaataata atacctgtaa atatagttgc taataaatcg ttcatttttt tctccctcta
     5881 tagtgaaagg ttttcttaat acatttcttt gctatcataa cataatgtta tctacttgaa
     5941 tagctgagcc ttggtttgga aaggtgaaaa aaggagtatg acaacagttg tgtcatactc
     6001 ccctttttcg aataaacggt gttaaacggc aggctcacag cctctttcat ttgcctaaaa
     6061 ttaattgctc ccatttcctc cgaaccatcc agttttacag ttgatggtta cgatgtaaaa
     6121 tggcaaagca ctttctccaa tgcctttcat caatgcagat tttgttgaag tggcatatag
     6181 attataatag ccttccccat tttcgattct ggctttttct aatctgtaac ctccttcaac
     6241 gatatatcca cgttggatac atgtagcaat cactttgctt tggacacctg gtgcccacgt
     6301 ccatgctgga ttagatgcat cggcaatatc acttgctctg gcagatttga ttggctgatt
     6361 cgctgagttt gaagaggaag gggtcgagga tgccgcttct tgctgtgctg cttgtgcgac
     6421 cgcagcttgt tgagcggctt gagctgcagc tgctgcttga gcggcttgtt ttgcttgttc
     6481 ttcagctgct tttttcgctt cttctgcttt tttcttttcc tcttcttctt ttttctcttt
     6541 atcagtgagc atcttatcta cttgtttcaa ttgttcattc aactgagcag atagatctgt
     6601 atttttcact ttttctactg cttcttttgc cgtcttgtat tggtcgcgtg ttgctgaatc
     6661 cactactttt ccgtctttaa agactatagc tgttttttct ttagctgttt gcaattcagc
     6721 atattgattt ttagcttctg tttgtgcttg ttgaatcaat tttccaaaat ctgtgttatc
     6781 cgtttcgatt gcttggatct ctttatctgc tttcaacttg atgtcatctg ctagatgatc
     6841 atcgacgatg gcaggtgcta tgaaccactc attcactttt tggatatctc ggatcttaga
     6901 ttggatatct tggtaagttg tttctaactc tgaatatcct ttttcattct taagactgga
     6961 taaatcattt tttagttttg ataaatcttg atgaatcata tctgcacgaa taaattgatg
     7021 gtgatcatct gtataaaagg cagcaagttt ctcatgaagt tcttccattt gttgctgtct
     7081 ttttgcttct gctgctactt cggcttctac tttttgatga tggtcataat aagcccagct
     7141 tcctccaccg agtagaagta ctgctgctaa aacgagataa ggtacttttt ttgatttttt
     7201 cttttgcgct ttatctggat ctgttttttc taagtttgat tcttgatgtg ttttttcagt
     7261 atctggcacg attgttattg atttcgcttc tgcttcaaac agcttaggct ggttctcttc
     7321 tttttctatt tttgcttcaa gatcagtacc ctctttgctt acagattgat tttcagcatc
     7381 ggattcacta tttatttcat cgggctctgt tgactttttg tctgtgtttt cgagtatttc
     7441 ttttttctct gcttccggca gtttattcgt tttttccttt tctaattgtt tttcagtaaa
     7501 tacttcagtt ttttctgtga tatccgtttt tacgtctttt atttttactg tttctttacc
     7561 tgtggcgttt tctctatcag agtctttttc agtttcttgt tccttttctg gctctacttt
     7621 gttttcctgt gcaatttttt cactgatcat tttgagcagc tctttcgcgt gctcttcttt
     7681 tgtatcttca cgatgtgctt tgatataagc ggatagtatc gaattttctt cctcattatc
     7741 gacgatttct ttcagttcag ctactcttct tttttgtttt gattcaagcg tttcttcttg
     7801 attctttcct gattcttccg aagtctcatg aacagttgct ttcttctgtt catttttctc
     7861 tatattagtt acttgatcgt gagcaggtgg ttcttcgtct tctggttctg ctttttcgaa
     7921 taattccatt acagattcta gaggtaaatc tttcaattcc gaccattcga ttggatcgtt
     7981 taattctgta tcaggaaaga ctgcatcttc agttgattta tcctgcatag ctgcatctgt
     8041 atttgtttct tcaattgatt cttttatttg ctgatcttct gcagttattt gatgaccgca
     8101 gtctggacag atagtattct gctccatttt tttcgatccg caatttggac attttttcat
     8161 cacttagcga tcggctcctt tactaggttt acgctaccat ttaatctatc atattctata
     8221 cgtcacgcca atagcttatt tttaggattt acttaaaaat aatgaatgaa tttccttcct
     8281 atagaaaaaa aagcttgaga ctgcgtagtc tcaagct
//
LOCUS       DAZUMV010000118         8251 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000118 DAZUMV010000000
VERSION     DAZUMV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.119"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..197
                     /locus_tag="ACG8FK_002306"
     CDS             <1..197
                     /locus_tag="ACG8FK_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097871.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="ACG8FK_002307"
     CDS             314..811
                     /locus_tag="ACG8FK_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5097872.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="ACG8FK_002308"
     CDS             898..1290
                     /locus_tag="ACG8FK_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5097873.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="ACG8FK_002309"
     CDS             1274..3721
                     /locus_tag="ACG8FK_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5097874.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="ACG8FK_002310"
     CDS             3724..5907
                     /locus_tag="ACG8FK_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5097875.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="ACG8FK_002311"
     CDS             5904..6905
                     /locus_tag="ACG8FK_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS5097876.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="ACG8FK_002312"
     CDS             6920..7834
                     /locus_tag="ACG8FK_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5097877.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8148..8195
                     /locus_tag="ACG8FK_002313"
                     /pseudo
     CDS             8148..8195
                     /locus_tag="ACG8FK_002313"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2569 a         1568 c         1754 g         2360 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg c
//
LOCUS       DAZUMV010000119         8251 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000119 DAZUMV010000000
VERSION     DAZUMV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.120"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..1559
                     /locus_tag="ACG8FK_002314"
     CDS             234..1559
                     /locus_tag="ACG8FK_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5097878.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1552..1902
                     /locus_tag="ACG8FK_002315"
     CDS             1552..1902
                     /locus_tag="ACG8FK_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097879.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1899..2069
                     /locus_tag="ACG8FK_002316"
     CDS             1899..2069
                     /locus_tag="ACG8FK_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097880.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2973..3248)
                     /gene="prgO"
                     /locus_tag="ACG8FK_002317"
     CDS             complement(2973..3248)
                     /gene="prgO"
                     /locus_tag="ACG8FK_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFS5097881.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3220..4173)
                     /locus_tag="ACG8FK_002318"
     CDS             complement(3220..4173)
                     /locus_tag="ACG8FK_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5097882.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            4785..6278
                     /locus_tag="ACG8FK_002319"
     CDS             4785..6278
                     /locus_tag="ACG8FK_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFS5097883.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            6413..6709
                     /locus_tag="ACG8FK_002320"
     CDS             6413..6709
                     /locus_tag="ACG8FK_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS5097884.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            6810..7661
                     /locus_tag="ACG8FK_002321"
     CDS             6810..7661
                     /locus_tag="ACG8FK_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097885.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            7682..7894
                     /locus_tag="ACG8FK_002322"
     CDS             7682..7894
                     /locus_tag="ACG8FK_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097886.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2866 a         1283 c         1552 g         2550 t
ORIGIN      
        1 tttttttcta atataactaa gcaaaactgg ataatacgac acttattttc aaattttcac
       61 aagactaata gtaaatatcc atttaataaa gtgatacaat gtgttgattt tacaaatcaa
      121 cagaagaagt agggaaataa cttacctatg cttctttttt tgtacattta atcgattctt
      181 ctttacaaaa acgaacgtat gtttgtataa tgtggaagaa tggagtgtta tttatgatgg
      241 attattcaaa agagccagta aatgattact ttttgatcga tatgaaaagc ttttacgcaa
      301 gtgtcgaatg tatagaaagg aatttagatc cattaacgac tgaattagtc gtcatgagtc
      361 gagcggataa tacaggatca gggttgattt tagcttcttc accagctgca aagaagaagt
      421 acggtatttc taatgtcagt cgaccacgag atctaccgca accttttcct aaaacgttac
      481 atgtcgttcc accacgcatg aatctctata ttaagcgaaa tatgcaggta aacaatattt
      541 tcagaaggta tgtagcagac gaagatttat tgatttactc gatcgatgaa tcgatcttaa
      601 aagtgaccag atcaatgaat ctttttacga ctgaaggaac aagaagtcaa cggagaaaga
      661 agctggctca aatgatccag gagaatatta aagaagagct aggattgctt gctacagtag
      721 gtgtcggaga taatccctta ttagctaaac ttgctttaga caatgaagca aaacataatg
      781 atggattcct tgctgaatgg acgtatcaaa atgttcctga taaagtatgg aatattcctg
      841 aaatgacaga cctttggggt attggatctc gaatgaaaaa aagactcaac caaattggta
      901 tcatgagtat taaagactta gctaattgga atccatacac tattaagagc catttaggtg
      961 tgattggctt acagctgtat tttcatgcga acgggatcga tcgaacagat atagcgatcc
     1021 caccagaacc gacaaaggaa aaatcatatg gtaattcaca agtgttacca cgagattata
     1081 ccaggagaaa tgaaatcgaa ctggtggtaa aagaaatggc ggaacaagta gctattcgta
     1141 tcaggcaaca taactgtaaa acaggttgtg tccatttata cattggcacg tcgatcttag
     1201 aaacgagacc aggtttttca caccagatga agatccctat aacagataat acaaaggaat
     1261 tgcagaatta ctgtcttttc ttgtttgata agtattatga aggacaagaa gtaaggcacg
     1321 ttggaataac ctatagcaag cttgtgtaca ctgattcgct tcagctggat ttatttagtg
     1381 atccacaaaa gcaaattgac gaagaaaatt tagataaaat tattgacaag attcggcaaa
     1441 aatatggttt tacgtcgatt gtacatgctt cttctatgtt agaaggcgct cgtagtatta
     1501 ctagaagtac attggttggt ggccatgcag gcggcgcagg agggcttaaa aatgattgat
     1561 tataatgatc gtggacgttt aaagtgggca ggtttttatt tatctgatca tactgaaaaa
     1621 attgatgccg acaatgaaca acgagccaat caaaatttgg caaaagaaca aatgacgact
     1681 gaagaggttg cggaagtatt gaacgctgca gtcataaaaa atagatcagt atgtattcaa
     1741 aaagaagaac gaaacgctga aggatactat ccagaagata ttgttagcaa gatcttaggt
     1801 agtgatgagc tggggatcta cgttggaaaa aacaaagttc actacgacga aatacgtcat
     1861 atcgaatttt accaggataa taaatggggt cgattagaat gaacattatc cagcaatatg
     1921 agctaaagta tataaccttt gatcagctat cagaagagat ttggggttat ggccagcgtt
     1981 tgataaatga ggttggtgtt gaacgcttct ctttctatgt agaagctgca gcaggatacc
     2041 ataaatacga atactatatt ggctattaaa gtgcaataaa attatatttc ttttatgttt
     2101 tacattttgt atcaattctg ataaaattac ctataggtaa ttaaaaaata taaaaaaact
     2161 agttacaacc ttaggacgtt gtaactagct tgaagcatac tagagctaac taaaatagca
     2221 ctagaaagca atgttaaacc cgtccaacaa acttgattta acattgagac cagcttattc
     2281 agctaaatat agttgaataa gcttcttttg ttttaaaaaa acaaaaggtg tactacgaat
     2341 aacgcaagta aaagaaatac tattacaacc agtaggctac aagtaagtat tactaaacat
     2401 tgcgtagtta gaacctttgc tctattgtct aaagccacaa aattagagat cagctttatg
     2461 atgctaagag cgagctgcat ccatgcggac ataggttctt ttgacactat taattctcct
     2521 ttttaggata agggaaattg ctagtagttt tttcattagc accatccccc ttcatttgat
     2581 tgagtctagt gctgctgtga tagtttgctg tttaatatgc ttacattctt attataacat
     2641 aaatgaaaca aacacatggt tagaggtcgt atcaatcaaa atagtgtctc acaaacgatt
     2701 tattacttac tcctgctgac taacggactg ttcttcagta actgtaacta tatgtttgtt
     2761 aaaaccacca ttacctgtag ataaaaacca atatatttac tatactcact atattaaata
     2821 tatttagtat agtaaaaact agaaacaata aaatttgtga atgttgtata cccccaaaca
     2881 atacacatat tttgaaggat taggattaat aaagctaaaa atatagtaag tatactaaat
     2941 atatttacta tatctagtat acttactata ttttaatctt ttgatcgtct attatatata
     3001 atccgttcct cgtcgtttaa agtatcaaca taggcgtcca ttgcaagttt gactatttca
     3061 tacataggaa tatcttttgc ataactaaga tcccgaatgg tttttagtac gggaggatct
     3121 acttgaactg gttttctttc tttcgcagtg tactttcgtt cccctaactc gacattaata
     3181 tcatctttaa ttgaaatatc ttcagcacca attttcttct tatctttgtt cgttggtaat
     3241 aatgccattt agtttaatct cctttccaag aggtaggata tgattggcca ttgagtcagc
     3301 atatttaggt gtataatcaa atccttcaat atcaccatgt tgttcatagt attcaattct
     3361 ttcttctaat tctgtaaaga tgtctgcaaa aatagcccac catcttcgat ctacataatc
     3421 atttaattgg ataccattct caccatacat ttctaaccga tccgagcctt taacaatgct
     3481 tttaaaaatg tttgattcac catacttatc aattgttttt tgatagtttg cgtgttgaga
     3541 ggaacgtcgc actgagaaaa gaacaggcaa aattccaatg atttgaagat gagactcttc
     3601 aaaattcgtg acaatcggaa ctaaaacttt attaataaaa tcttctgttc cttccattgc
     3661 gtaccgtttt aattcttgca ttgcaataat ataatctgca gcagttaaga atgcacgaac
     3721 cacattatct gttgatggtg ggcagtcgat aaagatataa tcgaaatttt ttctcaattc
     3781 ttctaaaggt ttaatgaagg ccaatgatct cttacgacga tcaggatatt tttcggacaa
     3841 gtattcatta agttcttcag atccaggaga gccagccata atatataaat gtggacttac
     3901 ttgagtaata cctgaaaccc agtccccttt tttgattgct gcagacatgg atcgatcaaa
     3961 gctagtaatt tgatacgttg atccaatgtt ggaagtagta tttctttgtt ggtcataatc
     4021 aattagcaac acgtcttttt caaaaagttg actagaaaca atcgctaata aatcattgtc
     4081 tgtagtctta ccaatgccgc cttttctacc tgtaagcatt acaacttttc ctagtttatc
     4141 tgatgtttta aatgcagtcc gtaagttttc catagttccc tccaatttcc tatactaaat
     4201 atatttagta tacttcatat acttaatata ttcgatatat ctaatatact ccaaattgat
     4261 aagaattcag actttataag gaatatagtg gatatactaa atatatttgc tatattcagt
     4321 atatcagata tattcaaaat atcaaaatat atttgtgcgt tttgcgttga caatttagag
     4381 tgaaaagagg taaacttaat tctatagaaa ccacagataa ttcactattt acaaagtagc
     4441 aaaagcagtg gtactcttta aacagaaaaa ttacataaaa aaaagagacg gaaataattg
     4501 attttagcag aatcaattat ttctgaatca catggttagg acatgtgttc acgctcgtct
     4561 caagaatatg taagtagcta ccaactactt atattaaaag ttgatcggat accttttccg
     4621 attgccttta ttctaccaaa aaaaggcgag caactcaaca gaagaacgtt tatttagtgt
     4681 acttaaatta aatattctta aacactgaag agagaacaca tactatggct gagattaaag
     4741 ctaaactgtg ttctcttttt gtttgagaaa ggacgttaca atcgatgttg agcgaaattt
     4801 attcagctat tcttaaaaac ggcttacgta catacaaata tcgaaacagc accatgaaac
     4861 ctgcaggtca tattgattcc agtaggagtg gtgctatctt tgcgtaccgt agcaagcaat
     4921 taatgaatgt tgggcgtggt atggtcatta cttcggaaga agctatttta gagaacgaaa
     4981 agaaactaac gcattggaca cctaatacct atcgctttgg tacttatgcg gatgattatc
     5041 gaacagtggt taaaggtcat tctgagaaaa acttaagtca aatcaacaca tttgtagttg
     5101 atatcgattc aaaagagaac catcaaggcg aaattatttt agcctgttta gatcaagtgg
     5161 gctacatgcc aacgctaatt ttagaatcag atcatggtta tcaagtttat tttgtactaa
     5221 agacaccagg ctatgtcaca aagaaaagca atttcaaggt gatcgatgta gcaaaaaaaa
     5281 tttcaaaaac aattcgtcta cagttggctg aaaaattgtc cggagtggat ctaggatgta
     5341 accacttcgg aattgctcga ttcccacaag aaaataatat tgtttttaaa gaaatgaact
     5401 atcagtattc ttttgctgac tggattcaat ggtcgatgaa gcttgaatca gatcaaaaaa
     5461 caaaagaaga tcgagaacga aaactaattg tctttcctga gaaaaaagaa attcgtcaag
     5521 tagatgaacc gtggttcgat atgttacttc ataaagcaaa catcgtcggc ggagaaggac
     5581 gtcttggtcg aaataatgtg attttcacgc tttctttagc ttattactct tcaggttatg
     5641 cattggatac ttgcgaatac aatatgttca cattcaacga acggttacag gaaccgctat
     5701 ctgaagcaga attagtaaag attgtacgta gtgcttattc tggtaattat caagcagcaa
     5761 gcagagaatt cattattgaa ttgtgccagc aatgggtttc accaacctta aaaaataatg
     5821 atctgttcat tcaacgacgt ggctggtgga aatttaaaaa gccacgagat caaagaaaat
     5881 atagtcataa aaatgaatgg aaagaagatc tattggctta tctaagcgaa aaaagtcttg
     5941 ctagtgagcc ttatttgaac ctgtcaaaaa aagaattggc ttcacaacta acgatgccca
     6001 agcgaacact tgataagatc ttaaatgagt tacgagagga gaataaagtc ttctacaagg
     6061 taacacgagg aagaaatggc ggaatgatca ttgcttcagt caaaacgttg ttcgcgaggg
     6121 ttattcaact aagtaaagaa aagagactag attattcaac aaggatcaca gaggtatttg
     6181 gactcaacga gagcttagta gaagagacat ttagacaatt atcaaccccc ttgaaccctc
     6241 ttaaagaagt gaatcttttt gaagtagata caggttgaaa aataaaaggt gcaaattgcc
     6301 ctatacaata tatctatgat acttctgtag gtgttgctcc ctttgcctag ctacgtgtag
     6361 gtggtatact tataactgcg tatgcagtct tatgtatcat ggaggaagtt gtatgtcgtt
     6421 aaaaaattat gtctattcac atcctgttaa tgtgtttatc attggtcgtt tgggattatc
     6481 tgtggaagtg ttctgtgagc tttatggttt taagcagggg actttgtctt cttgggttac
     6541 cagggaaaaa acagttgctt ctttgccgat tgagtttctt catgggttgt ccttagcttc
     6601 tggggagact atggatcagg tatatgattg tttgtgtgtg ttagagcagg agtatattga
     6661 gtataaaata gctaacgagt taagaaaaag gaagaagtat atccagtagt agtttttatt
     6721 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
     6781 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
     6841 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
     6901 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
     6961 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
     7021 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
     7081 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
     7141 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
     7201 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
     7261 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
     7321 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
     7381 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
     7441 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
     7501 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
     7561 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
     7621 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
     7681 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     7741 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     7801 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     7861 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     7921 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     7981 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     8041 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     8101 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     8161 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     8221 cgtaagagaa attgatcgaa gatgtgacga a
//
LOCUS       DAZUMV010000120         8173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000120 DAZUMV010000000
VERSION     DAZUMV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.121"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            341..1204
                     /locus_tag="ACG8FK_002323"
     CDS             341..1204
                     /locus_tag="ACG8FK_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5097887.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1333..2955)
                     /locus_tag="ACG8FK_002324"
     CDS             complement(1333..2955)
                     /locus_tag="ACG8FK_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5097888.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3204..3725
                     /locus_tag="ACG8FK_002325"
     CDS             3204..3725
                     /locus_tag="ACG8FK_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5097889.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3911..5641)
                     /gene="spxB"
                     /locus_tag="ACG8FK_002326"
     CDS             complement(3911..5641)
                     /gene="spxB"
                     /locus_tag="ACG8FK_002326"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS5097890.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5806..6624
                     /locus_tag="ACG8FK_002327"
     CDS             5806..6624
                     /locus_tag="ACG8FK_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS5097891.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6727..6924)
                     /locus_tag="ACG8FK_002328"
     CDS             complement(6727..6924)
                     /locus_tag="ACG8FK_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097892.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7093..7671
                     /locus_tag="ACG8FK_002329"
     CDS             7093..7671
                     /locus_tag="ACG8FK_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5097893.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7766..7903
                     /locus_tag="ACG8FK_002330"
     CDS             7766..7903
                     /locus_tag="ACG8FK_002330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097894.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2466 a         1454 c         1611 g         2642 t
ORIGIN      
        1 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttt
       61 tccgtttttt tctctaatac tcaggtcaaa acgctgattt cacaacctct gattcacggt
      121 gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaatttg agaagcaatt
      181 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct cttattttga
      241 ttagaacttg cgaaaataaa ccggttaaag tgtgtctagc gcaagagatt tgctataata
      301 gacgataagg gtaaaatgta acgagaaggg gttgaaaaat gtgtatcaaa ttgtaacaga
      361 ttcttgttgt gatctgccat accatgtatt agcaaaagaa aaagttgatt ttttatcaat
      421 gaatctccaa ttgaatgatc aagagttagt agatgacctc ggaaagacat tcaattatga
      481 ccgctacatg aatcagctga aaaatggtgc gatgcctacg acatcacaaa tcaatgttgg
      541 ccgatattta gaatttttcc gttcttatgc ggaaaaacag attcctgttt tatatgtggc
      601 tttttccagt ggattaagcg gctcttacag tagtgcgctt caagcggttg aattgctgaa
      661 ggaagaatat gaagaccctg agatctatgt ttttgataca aaagctgcaa gtattgggga
      721 agggctttta gtattggaag ctgtaagatt gaaaaaagaa ggtaaaagca tcgaagaaac
      781 cctagagtgg ctggaaagta attatttgaa agtccattca tgggtaacag tagacgatct
      841 gaaatatcta gaacgaggcg gtaggatttc caaagcaagt gctactcttg gcagtctatt
      901 gagtgtaaag cctattatta cggtggataa agaaggaagg ctgacgaatg ttgacaaagt
      961 tcgaggaaga aacaagtcga tccagacaat cgtcaatgag acggtaaaaa caatcgttgc
     1021 accacttgat caaatcgttt atgtggctta cgctggggat ctagagagcg cggagaaggc
     1081 aaaacggctt ttggaagagc agataaagat gaaagatgtg aagctttatc ccttaggacc
     1141 aacgatcgcc agtcacacag gctatggctg tatcgcgatt ttttcgatgg gaatcagtcg
     1201 ataaaagcag taactcattc tgtaaaaaag cccagaaatt gattgtaaca aaaacagcga
     1261 ctcttaaaag atagatgagc atcctatctc ttagaggtcg ctgttttttt attttttaag
     1321 ctaaataggc cattatgccc ataactcgtt caattgtttt tgaatatctg cattttccaa
     1381 aaattcatca tatgtcgtat cagctcgatc gatcacacct ttatccgaaa cggcaatgat
     1441 gcggtttgcc aatgtttgga taaattgatg gtcatgtgac gcaaataaga ttgagccagt
     1501 aaatgccatc aagccgtcat tcaatgctgt gattgattct aagtctaaat ggttcgttgg
     1561 atcatctaat acaagtacgt tagctttcga aagcatcatt tttgaaagca tgcaacggac
     1621 tttctctcca ccagataaaa cattcactgg tttcaatact tcttcaccag aaaacaacat
     1681 tcttcctaag aagcttctta agaaggtgtt atcatcttct tctttacttg catattgacg
     1741 caaccagtct aaaatcgtta gatcagtatc gaattctttc gttgtatcct ttggtaaata
     1801 agaattactt gttgtcacac cccaacgtac tgttccagta tctggcgtaa tttcacccat
     1861 gataactttg aatagcgttg ttgttgttat atcattttct gcgacgaaag cgaccttgtc
     1921 ttctttgctc aaattaaaag atatatcatc taaaattttc tttccatcaa tcgttacact
     1981 tacattttct acctgcaata agtcgtttcc aatctcacgt tcaggtttga atccaacgaa
     2041 tggataacgt ctagaagaag gttggatatc gtctaaagta attttttcaa gcattttttt
     2101 acgtgaagtt gcttgttttg attttgatgc attcgcacta aagcgggcaa taaattcttg
     2161 gagttctttg atttgttctt cttttttggc atttgcgttt gcttgaagtt tagccgctag
     2221 ttgactagat tccaaccaaa aatcataatt tcctacataa agtttgattt taccaaaatc
     2281 tagatccgcc atatgtgtac atactttgtt caaaaagtga cggtcatggg atacaacgat
     2341 cacagtattt tcaaaattga tcaaaaattc ttccagccaa ttgatcgatt ggatatccag
     2401 accgtttgtt ggttcgtcta gcaaaagaac atcaggttta ccaaatagtg tttgtgctaa
     2461 taacactttt accttttgtc caccagttaa ttcactcatt tgctgatggt gcaattcttc
     2521 agggatgttc agaccttgca gtaaaacagc tgcttctggt tcagcttccc atccattaag
     2581 ctcagcaaat tctccttcta attcagctgc tcgaatacca tcttcgtcag tgaaatcctc
     2641 tttcatgtaa atcgcatctt tttctttcag cacttcatac agacgcttgt gtcccatgat
     2701 gactgtttca agtacagtat agtcttcata gtcgtaatgg ttctgtttca atacggccat
     2761 ccgttcatct ggtcccatag taacggaacc tgtggaaggc tgcagatccc ctgataacac
     2821 tttaaggaac gttgatttac ctgcaccatt tgcaccaatc aagccatagc agtttccggg
     2881 ggtaaattta atattcacat catcaaatag tttgcggtct gaaaagtgca aactcacatc
     2941 atttacagta atcaattttt ttcctcactt ttatcattat tagttttgac aagtcttata
     3001 gaattatata ggggaaaggt aaaagattca aggcaatcga acattctttg tgtttcacgt
     3061 gaaacagaat gaaaaaaata aaaataaaag cggagcgaaa atagctacta taggatttat
     3121 tagtttgcaa cagcataatt tttgacgtta tttataagag ggagtactat aaaactgtaa
     3181 aagaaatgaa agaaggattg aaaatggatg aaaaaagaac catcgactgg ggctcattga
     3241 ttttgggaat tttatttgta cttgtctcat tgttatcatt tcaagaccct gtcggaaatt
     3301 tagttgcaat tgtagtagtc tttgcgattt ttgcctttat taaagggata tttgaactgt
     3361 ttgtacggaa cagattaaaa gagctaacag gatataaagg aaagatgccc ttaataatcg
     3421 ggatcatcga tattctagtt ggcgtctttt tcttattcaa tattggtgca ggagtcgcag
     3481 cattgccttt tgtttttgca gtatggttta ttgctgattc agttttagcg ttatttactg
     3541 ctgacttggc aagaggagta agtgaaggat attattggtt tacgatcatc gtcaatattt
     3601 taggaatttt attaggaatt ttcttattgt ttaatccaat ttcttctgcg ttgacattaa
     3661 gctttttagt tggattctat tttatgttat ttggtatcac acatatcgtc tatgcattta
     3721 gataagtaaa atcaaacaaa taaaaaagcc ggcttccgat tacagtttta ctgttgtaat
     3781 tggaagccgg cttttttatc atggacaaat atgcacgagg tgatttttag gtgtcgagac
     3841 aaatttatgc aaaaatgtga gaagctatat acacaaattt tatcttaata ctcgaagcat
     3901 agcgcagttg tcacaacctc ttttcatata attcattcaa tactggcaaa tcatggactt
     3961 catatttttc tttgaatgct tggatttctt cttcagaata ttttcttgga tctaaatgca
     4021 gatcttctac tggcaatggg cgcttgttat tgatcttcac atcgattact acaggacgat
     4081 ctgattttgc agctgcatca aaggcaaatc gaagttgcga gtattctgtg actgtaaagc
     4141 tatttgcccc gagcgcttct ccaactttac cgaaatctgc atcttctaaa aatacaccaa
     4201 atttcttttg ttccgtatct tcttgttctg cttcgatgaa accaaaagaa tcattcgaaa
     4261 agacaacatt aataattgga agctgatatt tgacttgtgt aatgatatct tgcatgacca
     4321 tagcaaaccc gccatcacca cttaaggtaa atacttgttt ttctgggtag cttagttgtg
     4381 cggcaattcc tcctggcaca ccattaccca tagttgcgaa ccatccagat gtcgtatgtt
     4441 tttgtttacc gttcatattc aataaacgaa tagaatggat agtcgtgtta ccgacatctg
     4501 tgacgaaaat cgcatcgtct tcagcaatac gattgatttc tttatagaca gcctctggac
     4561 gaatcggttc ttcctctcga tcttcaaaag atttcaacca atttacccat ttttctttat
     4621 tttcttgatt tgctaggtac cagctatcag cagggcgagc atcccctaac tcgacaagtc
     4681 tttgcaaagc tgtgtttgca tcaccgagga tcgacaaaga tgtttcatgg cgtcgtccaa
     4741 attttgacgc atcgatatcg atttggataa attcagcagt tggattaaaa aatgcacgtc
     4801 caaatgggaa gtcgctaccg acaaataaga tcagatcggc ttctgccaat gcttcatttc
     4861 cgggtttagt agcgacacgt ccagcaaaac caagaaagtt ttcataatca tcaggaataa
     4921 ttccttttgc tagaacggaa gcgacaacag gaactgaaaa atgttctgag aattctttga
     4981 tttgcggaaa tccgtttcgc gttccttgac caacataaag aactggtttt tttgcttttt
     5041 caagataagg caaagcagct agcaaatctt tttcttctgg taatatcaca cttgttttgt
     5101 gagtgtgagc ggtggagaaa ggttgttcgt caatttcttc aaaaccaaga tcaacaggga
     5161 tagtcacgat agccacgcct ttatgctcat aggcggcttt aatggcttca tctactacat
     5221 gcggaagact ttgtggtgtc atgactgtac gattgtatac gctaacatct gcgaaaatag
     5281 gattttcatt tagttcctgg aaagagttat agttcatgga tgtagaagca acttgtccta
     5341 agaacgctac tagaggcaca tggtccatct gtgcatcata caaaccattg atcaaatgtg
     5401 tcgcaccagg tcctgcagag ccgaagacag cgccgacttt ccccgttaat tttgcatctg
     5461 cagcggctgc gagtgcgccg acttcttcat gacgaacttg aatgtagttg atttcggttt
     5521 tttcttgata tagcgcatcc ataattgaat tgaaagatcc tccgggaatg ccgtaaatat
     5581 ggtcgatacc ccagctttca agaactttta ccattgcgat tcctgcattg atttttgaca
     5641 tggtgtttca ctcctttagc aatgatgata cataaatttt ttgtatctag agtatagaac
     5701 tttttatcga aaatcccaac taattcgtct tttcacaaaa atgtagtcat gtttttggga
     5761 atatgctaga gtagtacata gatagattca agagagggga atggcatggt tgaatggcta
     5821 ttgagacttg aaccttggca gcaagcactt gctgggacag cttttactta ttttatgaca
     5881 gctcttggtg ccgggttggt ttttttcttt aaagaaatca aaaaagaagt gctaaacttg
     5941 atgttaggat ttgcttctgg tgtcatgatt gctgcaagtt tttggtcttt gttagatccg
     6001 gctattacaa aagcagaaga aaatggagat attgcttggc tagttgtaag tattgggttt
     6061 ggtttaggcg gtgtgttttt atatatggca gataaaacac tgccccatat gcattttgga
     6121 ccacaacatg aagcagaagg attgcctacc catttaaaac ggacgatctt gcttgttttt
     6181 tctattacat tgcataacat tcctgaaggt ttagccgtgg gggtagcatt cggtgcagca
     6241 gcaactgcgg ataatccgac agccgctata ttagccgctg tttcggtggc gctaggtatt
     6301 ggtatccaaa acttcccaga aggagcagct gtatcaatcc cgttgcgaca agaaggtctg
     6361 agtcgtaaaa aggcctttgt ttacggacaa gcttctggaa tcgttgaacc aattgctgga
     6421 gtgattggag cgctactagt aacgaaagtg gagctgctgc ttccttatgc acttgctttt
     6481 gccgcaggag caatgattta tgtcgtggtg gaagaattga tccctgaagc acagcagaca
     6541 ttgtccagca aacgtcattt tgcagttttt ggcgtcatga gtggatttat cattatgatg
     6601 attttggatg tggcattagg ataaataaaa aaaatatata tgatatatca aatagggccg
     6661 cgaacttata tcgcgaccct atttttttag taagttatat cataatgaag tgaatggtat
     6721 catacattaa taatagtttt ttctatgtgt atttcctttg atccagcgaa caagccagat
     6781 gataagtgcg attgggatta gaactttgac cgcaaaccat aaaagcccgc ttaggacact
     6841 gacaaaaaga ctgatgacga agctagctgc aattagcact aaaagaacca tgaagatatt
     6901 taagaacata tttttttgac gcatacaaaa ccctcctctt gttcattctt tactatagaa
     6961 aaagtataac agggattgtt taaaaacacc tccaactgaa gactgatttt tacttcatct
     7021 acttaaatga tcaaacaatt gctttttctc tggtttatca tctaaaatag agcggaaaag
     7081 gaggagaata tcatgcctaa aaatcgtctt gaagcattta cagatgctgt aatcgcgatt
     7141 atcatgacta ttttagtttt agaacttcat gaacctaacg gagacacgtg ggaggcgctc
     7201 gctgcattag gttaccgatt ttttatctat attgtcagtt ttatcatttt agcgatttat
     7261 tggaacaatc atcatcattt atttcaagtt gtacataaga taaatggacg ggtcttatgg
     7321 gcgaacagtt tctttgtttt tgcgctttcg ctttttccgt ttgcaacttc atggatcagt
     7381 gagtacatca atagcctggc tccagaaatg acttatggtt ttgttacctt gaccgctaat
     7441 attgcgtatt atatcttggc aaaagaactt gtcaaagcaa ataaaggcca cgagaaagca
     7501 ttgaaggcga tgttgaataa ttacaagaaa tcgtatctat cgattggttt gaatgtgtta
     7561 ggaattgtct tagggtattt agtagcgcca gtagccgtgc tgatcgcaaa catcgtgatt
     7621 ctagctgctt ggatcatccc aagtcgtacc atcgaatcac aatatcggta aaatgatccg
     7681 tgtttaaaat ttaagagaaa aatatagaaa aaataaataa acgatcaaat atccgagtga
     7741 tactagtatt caaagaggga tgcagatgga tcaaaaaaga attcatgtag gactgattac
     7801 tagttttgtc acgatgctta tttatttgga tcaaggtcat ttgacttgga cagtagttgg
     7861 tgtcgtagga tttttgacat tattcacagc aataaaaaaa taaacatctt tatgtaatct
     7921 gatgttctat ctatttaaga aaagcatgtt tacagcagta tgattctgtt gtaaacgtgt
     7981 ttttatttgt ggaaaattta atttttagaa aagtgtatcg aaaaatagat agttagtttt
     8041 tccacagtgg ataacttcgt aagagataaa aataaaaaat tagtcaaaga agggcgtata
     8101 gatttctttt gataggagat atacaacaga aatgaataaa tattaacgtg ttttcaaaca
     8161 gtataaaacg gtg
//
LOCUS       DAZUMV010000121         8066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000121 DAZUMV010000000
VERSION     DAZUMV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.122"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..883
                     /locus_tag="ACG8FK_002331"
     CDS             <1..883
                     /locus_tag="ACG8FK_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS5097895.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="ACG8FK_002332"
     CDS             931..3060
                     /locus_tag="ACG8FK_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS5097896.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="ACG8FK_002333"
     CDS             3035..3724
                     /locus_tag="ACG8FK_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097897.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4817)
                     /locus_tag="ACG8FK_002334"
                     /pseudo
     CDS             complement(4132..4817)
                     /locus_tag="ACG8FK_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(4953..5147)
                     /locus_tag="ACG8FK_002335"
     CDS             complement(4953..5147)
                     /locus_tag="ACG8FK_002335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097898.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5197..5637)
                     /locus_tag="ACG8FK_002336"
     CDS             complement(5197..5637)
                     /locus_tag="ACG8FK_002336"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS5097899.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5641..6486)
                     /locus_tag="ACG8FK_002337"
     CDS             complement(5641..6486)
                     /locus_tag="ACG8FK_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS5097900.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6635..8053
                     /gene="pepV"
                     /locus_tag="ACG8FK_002338"
     CDS             6635..8053
                     /gene="pepV"
                     /locus_tag="ACG8FK_002338"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS5097901.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2480 a         1515 c         1713 g         2358 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagctgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaagctt ttttattttg agcgatcact gcctgatcgg tttgttctag tattttttct
     4501 agtttagctt gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc
     4561 aacttttccc gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa
     4621 gtgccggact gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct
     4681 cgaatcggtg tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt
     4741 ccgggtgcta actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc
     4801 aaattattga taaccattta accttccttt ctatgcagat ataatctatt ataacataaa
     4861 tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg
     4921 cctattagag cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt
     4981 ttgacacgac cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg
     5041 agcagattgt ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc
     5101 attactttaa cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact
     5161 cgacacaata caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga
     5221 agcgtacgct tggaataact gttctaactc cggattgact ttgctcagct tcactaatcg
     5281 ttgcgaatca gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc
     5341 ttcagttccc acaatttcgt agctaaaatc caaccgtgtt cccgtatact tttggatagt
     5401 tgggtggatg ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc
     5461 cagaacgggg atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac
     5521 caataaggct acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc
     5581 catttgatct gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac
     5641 tcatgcctcg ttttccattg attttttaca aaaataattc atccacttcg gaagtgcttc
     5701 gctgatttgt gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact
     5761 atgaagataa acagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc
     5821 tgtgatcatc ccagctaaag tgtcacccat gccgcctgtt gccatagcag cagaacctaa
     5881 aggattggtg tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag
     5941 cacagcaccg atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg
     6001 tagagaactc aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata
     6061 gggtagcaaa agatgattag caacaagtaa acttaaagca gagccatcaa tcaccagcca
     6121 ttgttctttt gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt
     6181 acccaatccg ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc
     6241 agtatccgtc caatctacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt
     6301 tttttcatca gttactacag tcgtgagacc cgctccactg tgtacggcag ctaaagcact
     6361 cataatgatt gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt
     6421 tcctttatgt gaaatctttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat
     6481 cagcatcgaa aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga
     6541 taagaaaagg aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga
     6601 acacgaagac ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt
     6661 agaagcacgt aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga
     6721 gcgcgatgat tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt
     6781 gaaacatatg ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta
     6841 tgcagggcat attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga
     6901 tgtcgttcct gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg
     6961 aaagatcttt gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc
     7021 gatgaagatc attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg
     7081 cagcgatgaa gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc
     7141 agattttggt ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt
     7201 ttctttagct ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt
     7261 ttccggactt cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag
     7321 tgcagatgca gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt
     7381 aagcggaacg attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc
     7441 acatggtgca agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga
     7501 taactatgaa ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca
     7561 tgaagatttt tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt
     7621 gacaatgaat gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat
     7681 caatatgaat ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga
     7741 acaaacagtg gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca
     7801 ttacgtacca atggatgatc cattggtggc tacattattg caagtctatg aagaccacac
     7861 tggagaaaaa ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg
     7921 tggcgttgct tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga
     7981 atttatgagt cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg
     8041 tttggcgaaa taaaaatact aaaaaa
//
LOCUS       DAZUMV010000122         8019 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000122 DAZUMV010000000
VERSION     DAZUMV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.123"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            387..575
                     /locus_tag="ACG8FK_002339"
     CDS             387..575
                     /locus_tag="ACG8FK_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS5097902.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            653..1666
                     /locus_tag="ACG8FK_002340"
     CDS             653..1666
                     /locus_tag="ACG8FK_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5097903.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1733..2767)
                     /locus_tag="ACG8FK_002341"
     CDS             complement(1733..2767)
                     /locus_tag="ACG8FK_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5097904.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2881..5106
                     /locus_tag="ACG8FK_002342"
     CDS             2881..5106
                     /locus_tag="ACG8FK_002342"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5097905.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5124..7007
                     /locus_tag="ACG8FK_002343"
     CDS             5124..7007
                     /locus_tag="ACG8FK_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS5097906.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6988..7962
                     /locus_tag="ACG8FK_002344"
     CDS             6988..7962
                     /locus_tag="ACG8FK_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS5097907.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2584 a         1464 c         1699 g         2272 t
ORIGIN      
        1 ttttagttag tctacattat tttggcgatt tgccaataag gactgttgag ttgtgcaaaa
       61 tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt tcaatttgat
      121 taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa cactaaatat
      181 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
      241 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
      301 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
      361 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
      421 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
      481 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
      541 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
      601 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
      661 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
      721 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
      781 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
      841 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
      901 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
      961 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
     1021 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
     1081 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
     1141 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
     1201 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
     1261 tagtctgaaa ggaaccattt cttattgggg acctgttgat ttgaagctaa cagcacagca
     1321 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
     1381 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggccg accatgtttc
     1441 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
     1501 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
     1561 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
     1621 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
     1681 gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc tttcaatacc
     1741 tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa gtcaaaatga
     1801 ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct gcttgcccaa
     1861 ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg attgtctcga
     1921 tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt tgtttcaatg
     1981 cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta ggaagagggg
     2041 attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc cagccaccta
     2101 gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga taggcacgca
     2161 tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg actttatatc
     2221 ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt acggttttag
     2281 tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca gggttatcca
     2341 cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc gtaacagtcc
     2401 caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga ttatcagata
     2461 gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc atccctactc
     2521 gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta gagatcacac
     2581 caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa tccaacgcag
     2641 cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc tgatcgttat
     2701 tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca tctctaattc
     2761 caaccatttt tacacactcc tcaaaaaaat aaaaaatttt tattgacaaa attgtttagt
     2821 aaacagtata caggaattat aaaaaaagta aacggtttcc gttttgttgg gagtgaaatg
     2881 atggatatta cgcatagaaa aacagataaa aatttcatct tatttgatga aaagagccga
     2941 gtcttccatt tacgaaatac atttctttct tatctgataa agatagaaga aagcaatgta
     3001 ttggcgcata tttattttgg caaaccagtc aagcagtaca aagacaataa aaattatccc
     3061 cgaagagatc gtggattttc aggaaatgtt cccttaaatc ctgaccggtc tctgtcgaaa
     3121 gatacactac cgcaagaata ctcttctcat ggcagcatgg actttcgaac gcccgcatcg
     3181 atcattcagc gcaagaatgg atcagactta ctggatctga gatatgacag ccattatatt
     3241 acagatggga agccagagat cgaaggcttg cctcaaactt atgtgatgga caagtcagag
     3301 gcacaaacgt tggtgatttc acttaaggat agagaaacag cgatttattt cgatttgttc
     3361 tataccatat ttactgatag agcagtgatc acacgatctg taaagataag aaatgaaacc
     3421 ggagaaacaa tcaaactaga aaaagcggct agtttccagc tagactttgc ccatactagg
     3481 cgatttgatg aagtcattgc tttgccagga gcacatgtca atgaaagaca gatttcacgg
     3541 caatctgttt tatctggaac aaaagtattt gaaagccgtc gaggaacgag cagtcaccat
     3601 atgaataact ttattgcttt agttcatcat catacgacag aaaatactgg agaagcgatc
     3661 ggacttcaat ttgtttattc cggcaaccat tctttcgaac ttgaaaaaga tcagatcaat
     3721 caattgagag tcgtcggtgg gatcaacagc catcgttttt cgtgggaatt gaatgcaggc
     3781 cagtcttttc aaacacctga aatgatcctt tcttactcaa gtcagggatt gaacaaaatg
     3841 agtcagatcc accatgagtt gctacgtgaa agaatcgcaa gaggtcgtca tcaatttgct
     3901 gaacggccga ttctggtcaa taactgggaa gccacttact ttgacttcaa tagtgaaaaa
     3961 atcaaggcga tcattgatga agcaaaggag ctaggcatcg aaatgttcgt attagatgat
     4021 ggctggtttg gaaaaagaga tgcggacaat tcttcattag gagactggtt cgagtatgaa
     4081 ggcaagttga caaatggatt acgtgaaata gcagattatg ctcatagcaa aggattgaaa
     4141 tttggtttat ggtttgaacc tgaaatgatc tcggttgatt ctgaactgta tcgaacacat
     4201 cctgattttc ttatgcagga gccaggtaga atgccttctg cttccagatc acaacatgta
     4261 ctggatttta caaggttaga tgttcggcag acgattgaga aacaaatgag aaagatttta
     4321 gatacgattc ctcttgacta catcaaatgg gatatgaacc gttcgctttc agatgtctat
     4381 tcgattaccc ttgacccaca gagacaagga gaagtagcgc atcgttatat gctcgggctt
     4441 tatgaactac ttgaacattt gacaactgat tatccggaaa tcttatggga aggctgttct
     4501 ggtggaggtg gacggtttga tgccgggttt atttattata tgccgcagtc atggacaagt
     4561 gataacacgg atgcagtgga gcgtatgaaa atccaatatg gaacaagcct tgcttatccg
     4621 atttcgtcca tcacagctca tgtatcagct gtaccaaatc atcagaccga cagatctact
     4681 tcgttgaaaa caagaggaga aacagctatg agcgctgttt ttggttatga actggatttg
     4741 acaaagctgt ctcctgaaga aaaaaagcaa gtaaaagacc aaattatatc ctatcaaacg
     4801 attcgtccgg tgatccagta tggccattat tatcgtttgg cgagtccttt tgaagaaaat
     4861 attgccgcat ggatgtttgt gtctcctaaa caagatgaag ctattgtgtt tttagggagg
     4921 atactagctt cagctcaacc agcgttccat gaagtatatc tgatggggtt agatgatgag
     4981 gcactttatc aggaacagac ctcgaagcgg atattttcgg gggccgaatt gatgacagtt
     5041 ggactttact tccccgattt tcaaggtgat ttccaaacag aactgcttca tttcaaaaag
     5101 ttatgagaga gaaggaaaaa agtatgaaag caaatataaa atggttagat gatccacaag
     5161 tttttagagt gaaccaatca aatgcacata gcgatcatgt ttgttatgca acaagggaag
     5221 aagtagaaac cacatcctcc cttaaacagt ctttaaatgg atcatggcgt ttttgttatt
     5281 cgccaaatcc ttcggtacga ccaaaaacat tttataaaaa agattttgat gcctgttctt
     5341 ttgatgaaat cactgttcct agtcatattg aattagcaaa ttatgacaag atccattaca
     5401 tcaatacgat gtatccgtgg gagggacatg tatttcgccg tccccctttc acgacaagcc
     5461 ctgagaaaga ggcggaaggg atcttttcag ctgctaaaga caatccggtt ggttcgtatt
     5521 tgacgacttt tcgattagaa gaaggtctaa agggaaaaca aatctcgatt cattttgctg
     5581 gtgtagaaca agcaatgttt atttggttga atggtcattt tatcgggtat gccgaagatt
     5641 cgttcactcc gtctgaattt gatttaacac cttatttgga agaagaaaat attctagctg
     5701 tggaggttta caaaagaagt acagctgcat tcttggaaga tcaggacttt ttccgatttt
     5761 ttgggatctt cagagatgtc ttcttagttg ctcgaggaca aaaccattta gaagatcttt
     5821 ggatcaaacc tcgcttagat gacaaatgtc aaaatggcaa acttgaagta gatttgtctt
     5881 ttgagcaagt agccaaaaac agtcagctga atgtagcgat actagatcct gatgggaagg
     5941 aaatctatca acagcaatat ccgttaaaag agcaaatgac gattcgcatc gatggatttg
     6001 aaaatgtggc actgtggggg cattttcagc ccaatttata taagcttgaa ttggagacga
     6061 tagacgaaga aaaaagagta acggagtata ttgactattc cttcggtttt cgtaaaatcg
     6121 aaatcaaaga taaaatcatc tacttcaatg gaaaacggat gattatcaac ggggtaaatc
     6181 gacatgagtg gcatccgaaa aaaggacgcg ccatcactat agaagatatg gaaaaagatt
     6241 tggctatcat aaaaaatgca aagatcaacg gcgtacggac atctcattac cccaaccaga
     6301 tcacttggta ttatttatgc gaccaagcag gaatccatgt gatggcggaa acgaacctcg
     6361 aatcccatgg ctcatggcaa aaaatgggcg catttgagcc ttcttacaac gtaccaggtg
     6421 cagtacctca gtggcaggcg gctgttcttg atcgagcaaa aacgaatttc gaaacattaa
     6481 aaaaccatcc aagtattctt ttttggtctc ttggaaatga atcttatgcg gaagaaaaca
     6541 ttcgacaaat gaacgattac ttcaaaaaag tagaccccga tcgacttgtc cattatgaag
     6601 ggaattttcc aaatccagca ttcgaagatt cgatttcaga tgtaaaaagc tgcatgtatg
     6661 cgccaccaga agagatcatt gactaccttg aaaataaagc ggaaaaaccg ttcatcttat
     6721 gtgaatatat gcatgacatg gggaattcgt taggtggaat ggattcttac atgacgttgc
     6781 ttgatcgtta tgaaatgtac caaggaggct atatatggga ttttaaagat caggcgatcc
     6841 aagtgactga tccaatcaca ggcagagaag tgatgcgcta tggcggtgac tttgacgata
     6901 aaccaagtga ctatgaattc tcagggaatg ggatcgtttt tgcagatgga caagaaaaac
     6961 ccgccatgca ggaggtaaga tattattatg aaaaatacag taaataaaag tcatatggat
     7021 acggaaaaag ttgcaatcgt cttcggcgac tgtacattag gtgtcaaatc ggggaatacg
     7081 cattatattt tttcttatac aagaggcgga ctggaatcgc tcaataaaaa cggaaaagaa
     7141 tggctatacc gggaaacact tccagttttt tggcgagcaa cgaccgataa tgacagaggt
     7201 agcggatttg gatttaaatc cggtatgtgg ttaggagccg gattgtatcc aaaagtcaaa
     7261 gaaatagaag tactggtgga caataaaaag atcgatttgc cgattgctcc agtaaataat
     7321 caatattcaa gtaatgaata cgctgaaacg gttgaaatca tttttacact ggaaatcaac
     7381 acagtaccaa aaacccaagt gacaatttcc taccaaatcg ctggagaggg taaaatgctt
     7441 gtcaatatgc attattatgg acaaaaagga ttaccggaat tacccgcatt gggtttaagg
     7501 ttcattatgc caacgaaagc aaaatttttt gagtatgaag gcctatcagg ggaaacatat
     7561 ccggatcgga tggctggtgg tgtcgaaggt atttataaag tggcaggact ccccgtttcc
     7621 ccataccttg ttccccaaga atgcggcatg catatggaaa cgaaatgggt agaactaaca
     7681 agaaatcaaa cgttggataa tacggataaa gatcaagaag atttttcgtt gcgatttgaa
     7741 cagcaggata cgccatttgc tttttccgct ttaccattca aagcagaaga actggaacag
     7801 gcaacacata tagaagaact accgccagaa agacgaacgg ttcttacgat ttttgcaaaa
     7861 gtaagaggag tcgggggtat caatagctgg ggagcggatg ttgagtcacc ttaccgaatc
     7921 agcagcgaag aggaccatga aatcggcttt attattcagt agcaatcttt agattattat
     7981 ttgaaaaacg gagtttacaa gacaaagctg ctttattct
//
LOCUS       DAZUMV010000123         7929 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000123 DAZUMV010000000
VERSION     DAZUMV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.124"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..526)
                     /gene="yajC"
                     /locus_tag="ACG8FK_002345"
     CDS             complement(146..526)
                     /gene="yajC"
                     /locus_tag="ACG8FK_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS5097908.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(623..1768)
                     /gene="tgt"
                     /locus_tag="ACG8FK_002346"
     CDS             complement(623..1768)
                     /gene="tgt"
                     /locus_tag="ACG8FK_002346"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS5097909.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1986..2684
                     /locus_tag="ACG8FK_002347"
     CDS             1986..2684
                     /locus_tag="ACG8FK_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5097910.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2852..5491)
                     /locus_tag="ACG8FK_002348"
     CDS             complement(2852..5491)
                     /locus_tag="ACG8FK_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5097911.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6110..7141)
                     /gene="queA"
                     /locus_tag="ACG8FK_002349"
     CDS             complement(6110..7141)
                     /gene="queA"
                     /locus_tag="ACG8FK_002349"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS5097912.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7334..>7929
                     /locus_tag="ACG8FK_002350"
     CDS             7334..>7929
                     /locus_tag="ACG8FK_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5097913.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         2244 a         1717 c         1457 g         2511 t
ORIGIN      
        1 aaagttattt attattattt atatgacaca cccgattatt caatattgag caatatatcc
       61 catgctctta tctctttctt ttttgaataa aaaatgaggt aggacatctt tctgccctac
      121 ctcgtcttta aataagcttt gatacttatt tttcttcctt ctcttctgtc tcttccagcg
      181 tttctgttgt ttcagttttt acttcaactg tctcagctgg ttcttctgtt gtttcaacag
      241 tagtcgttgc atcatttgtt acgacagtcc ctggtttgac cgttttgatt gctgcacgat
      301 cgtactcaag gaagatccct tcgcagtcta aaacaactgt ccgtttgtca ttgtcgattt
      361 ctgaaacaac tccatgtaat ccgccgatcg ttacgactga atcacctggt ttcatgctat
      421 ctaacaagtt ttgacgttct tgctgttgtt tcttttgcga acgtgacata aaaaaccaca
      481 ttgctaatag cgcgataaac atcacaagca ttggtaatgt tcccattttt ttcacctcac
      541 taattctgta tctacaaaaa acactttagc agaaaaatgc tgataacact agttttttac
      601 ttaattttta aaagattttc ctttagaagc tttttgcgtt ttctttattg aacccgtatt
      661 cttcaaaaaa ggcttctcta aattcaagca aattatcatc catgattgct tgacgtactt
      721 gtttcattag attcaacaag aagtataaat tgtggtaaga agtcaaacga atcccaaatg
      781 tttcatcaca tttgatcaaa tggcggatat aggcacgtgt atagttttta catgtataac
      841 aatcacattt ctcatcaatt ggacggaaat catgggcata tttcgcattt ttcactacta
      901 gacggccttg agaagtcata catgtgccat ttcgagcgat tcgagtaggc aagacacagt
      961 caaacatgtc tactccacgg ataacaccat cgatcaatga atcagcagca ccgacaccca
     1021 tcaagtaacg tggtttgttt tcggggatca acggtgtagt gaattctaat acacgattca
     1081 tttctgattt aggttccccg acagataacc cgccgattga gtaaccaggg aaatccatcc
     1141 cgactagatc tttcgcgctt tgacgacgaa gatcttcaaa ccctgctcct tggatgattc
     1201 caaataatcc ttgacggtct ggattagcat gagctttcaa tcctcgttct gcccatcgag
     1261 acgtacgttc tactgatttt ttcacgtagt cgtaactttc atcgaatggt ggacattcgt
     1321 caaaactcat catgatatca gagcccaatt tattttggat gttgattgct ttttctggtg
     1381 ataaaaacat acgcgaaccg ttcaagtgat ttttgaaatg cacgccttct tcttcgatat
     1441 tccgcatatc acttaatgaa aaaacttgaa agccgcctga atctgttaaa atcggttgat
     1501 cccagttcat aaatttatgc agtccgccag cttcttctac taagtcttca ccagggcgta
     1561 gccataaatg atatgtatta cttaaaataa cgccagcacc catttctttc aactcttctg
     1621 gggacattgt ttttactgta gctaatgttc ctactggcat gaacattggt gtggggaaag
     1681 ttccgtgtgg cgtgatcaat tcccctaaac gcgcgccagt atgtttttct ttcttgatta
     1741 aacgatagcg aatggcaggt tgcgtcattc atttccctac tttctatata aaatatcttg
     1801 acaactccca ccatcataat cgtttcaagt gctttttgca agttccctcg gcttccttct
     1861 actttattta atattctttt ttttatcctt tttttatgaa taacaaaaaa aagaaaaaca
     1921 ttatgacaga aatatttttt gatacatggt atattggtgt tattaaagaa aaggagggaa
     1981 aaatcatgca atcttctgca gaattaaaac gccaagcaaa agagagtcta aaaggacgtt
     2041 ggggacaagc tgttttacta aatctaatcc ctacattgat tggtattgct actattcttc
     2101 ttattatgat tcctattatc ttgtttgcca ccaccatata tgatggatca gcgagcgtct
     2161 cagaagcgac aacttcagga ggcggtagtt caggaggcgg tggcggaatt atttccagca
     2221 tcattagcgc tttgttcatg agcggtattt cttggactta tcttgatatt ttacgtggac
     2281 aaaaagatca gatcgaacca ttcaaagatg catttcgtgg attcaaaggt ttgtttatcg
     2341 gcggagtgat cagcttagct gtgctgatct cgatctttac aacattatgg ggtttgctat
     2401 tagttattcc tggaatcatt aaaagctatt cttattctca aagctatttt atttactacg
     2461 atatcgtcca agaaacagga caaacaccta aagtactgga tacgatcact gcaagtcgaa
     2521 aattgatgga tggttataaa ggacaacttt tctggttaga tctttcattt atcggctggc
     2581 atatcttagc attagcaact gctggtattg gttatctatg gctcagccca tacatttctg
     2641 caacaaaagc agcattttat gaacaattgc caaaagatat ttaaaagaga gtgtgacttg
     2701 tgaccgaggg ggtggcagga gaacaccgtt tatcgagagg cagtaaaaga aacattcagc
     2761 ccattagcaa aaaggaggtg tgacaacatt catgtcacac ctcctttttg tatagttcca
     2821 gtaaaaagaa aatcaactta gaaacagtta atcattcctc ttcttctgct tccggtaatc
     2881 gatggaactt gatcagttca ttcaccgcga ttggtacgat agataaaatc cctaccaaga
     2941 accagtgatt caaactgatg tgtactaggc cgaatagttc ttgcgtaaat ggcagcaaag
     3001 caacagccgt tgtaatagct aatgccagta acgccatttc taccaaagct ttattggtcg
     3061 ctaaattgac tttgaagatc gattggctgc tgcgtacatt gaacacatgc agaatcgatg
     3121 aataagctaa gatcaagaat gcaacagttt gaccgacatg ctgacttgcg tctacccatg
     3181 ggctgatagt atcgacataa ctacctaaat aataaccaaa caaggttacg atggtgaaga
     3241 cccctgcatt tatggcaatt ttttgcaata aacctcgagc aaagatccct tcatttttcg
     3301 gaatgggtgg ctgatccatg atgccgtgtt ccgctggctc tcttcctaaa gcaaatcctg
     3361 gaagtccatc agcaactaca ttgacaaaca gtagttgcac agctgtaaat ggtgctcccc
     3421 atcctaacag catcgcaatc aaaacaataa agatctccga aatattacag cttagcaaaa
     3481 agttgatagc tttacgaata ttctgataaa ccgaacgtcc ttgactgaca gcgtctacga
     3541 tcgtcgcaaa gttgtcatcc gtcaaaatca tatctgcggc actttgcgcc acatctgttc
     3601 ctgtgatccc catggcacac ccaacgttac ttgctttcaa cgctggtgca tcattcacac
     3661 catccccagt catcgctacg actgcacccg tacgttgcca agatttgacg atccggatct
     3721 tgtcttctgg cgttacccga gcatagacag agagagcttt gacacgcgca tctaattctt
     3781 cgtctgataa ttgatgcagt tctgatcctg aaagtgcttc tgacggatca ttcaagatac
     3841 ctaattcttt cgctatggca ctggcagtca ccacatgatc tccagtgatc atgactgttt
     3901 tgatgccagc ctttttcgct cgcttgattg ctcctttact ttctggacgt ggtggatcga
     3961 tcattccgat cagaccaatc aaacgtaagt ctttttccag tgcttcagaa gaaatatctt
     4021 gcggttcttc gtcataagta gcataaccaa cagcaatcac tctcagtgcc cgtttaccaa
     4081 agcgatcatt aacgatcgcc gcttgttcta catcgcctga actaaagcgc ggagcaagaa
     4141 catcgaatgc tcctttggtg attgagatgt atttttcgcc catttggtga actgttgtca
     4201 tcatttttcg ttcagaatca aaaggaagct cgtttactcg tggatatttt tcttctaatt
     4261 ctgcctttgt atgataattc tcttcaacag cccttacgat ggctacttct gttggattac
     4321 ctttgataac aaggtcgtca tcttcttgct cgaccgtcac atctgtacat agtgcagcca
     4381 ttttcaatat ttccatcgcg ctgttcgtca ttgaatcttc gatattcgtt acttcatctt
     4441 ctttgctcca tacccggcgg acgcgcattt cattttgtgt cagcgttcct gttttgtctg
     4501 agcagataac attagcggtt cctagcgttt caactgctgg caactgtcgg atgatcgcat
     4561 gtttcctcgc catcttctgc acaccgtatg caagtgtcaa cgtaacaata acagtcaagg
     4621 tttccggtac agctgcaaca gccaaggaaa tcgaagtcat gaacatctca aggagtggtt
     4681 ctccttgcag ttcaccaatc acaaatacta aagccgcagc cgctaaagcg atcatactga
     4741 ttcgtttgcc tagctgattc aatcggacct gtaaaggtgt tttttgcata gtattttcgt
     4801 tcaaaagatc cgcgatctta cccatttctg tagccatgcc aatggcagtg acgatcgctt
     4861 tcccacgacc tgcagcaacc gtacatcctt taaataccat attaagttgg tcacctaatg
     4921 actcatcttc ttcagcaata aatgctgcat ctttttcaac tgcttcactt tctccagtca
     4981 acgccgattc ttctacacgt aattgtgatg cttcgatcaa acgggcatcg gcagaaatag
     5041 catcaccgga ctctaaaaca agaatatcac ccactaccag ttgctctgac tcaacagtgg
     5101 tcactttgcc gtctcgtatg acagttgccg tttgtttatt catatcttgc aaggattcca
     5161 gtgctttttc tgcgtttcct tcttggacaa tcgccaatac cgagttgatc acaacgatag
     5221 cgatgatcag cagtccttca aataaatccc catgttctgt agcaaatgcg gtataaaagg
     5281 aaatcaccgc tgctacaaga aggatgatcg ttgtaaaatc ggacaagctt cgaaagaatt
     5341 ttttgatcat cgattctttc ggcgcctcat cgaatttatt caatccctgc ttttttcgcc
     5401 gttcatccac ttcttgtgaa gacaacccag tatctggatc tgcttctgtt accttcttta
     5461 cttcttctat tgtctgcttg tattcttcca tcctattcct cctttaaaat cttcgtacaa
     5521 aaaaaggtac ttattttact ataacatcct ttttttattt acttcaaata aagggcttat
     5581 cgattatttg ttaagaaagc gtaaaattta aagtgcttat atttgcaaac gctaccaaag
     5641 gatgctatta tatagatgta aggaaggagg ataccatcaa atagacagaa gaatttctcg
     5701 atagaacagt gtaagcaaac aaagttgtta gaagtacagg tttcacccag acatgcgtgt
     5761 agagtacaaa cctataaata gtgaatgcgt atggatgttt ggatcacgaa gtatccgaaa
     5821 caagaagtaa tgcgatacgt aacaattgaa taacgtaaac gtctgaaaat tgaataggaa
     5881 caagagatgt ttcaaatgtt gaagatgtaa cgaaaacaaa atttttgttt aacatgaaga
     5941 aattcaacac cgtgctttgc atgtatgatg gaaagttaca atcatttctg tttatttgaa
     6001 tccttccgag gtacaagaaa gccgagctta tcgagagctc ggctttttct ttgtcataaa
     6061 atctttgtaa agcttttttg ttattcaaaa ctagcggctt ttcataacct tatttcacga
     6121 acatcgcatc tccaaaacta aagaaacgat agcgctcttc aatcgcatgc tggtaagcag
     6181 ataaagtcaa ttctcttcct gcaaaggcac taactaacat gactaaagtc gattttggca
     6241 aatggaaatt agttgagaaa gcttctacca ctttgaattc atagccaggc gtaatgaaaa
     6301 tatcagtcca tccgctgtct gctttgattt ctccattaaa tttcgttcca atcgtttcta
     6361 atgttcgaat tgaagttgtt ccgacagcca cgattttccc gccattttta cgtacttggt
     6421 tcaatcgctc agccgcttct tccgtcaaac ggtagaattc actatgcata tggtgttctt
     6481 caattttatc tacgctgaca ggtcggaatg tacctaaccc tacatgtagt gtcagataga
     6541 ctaattcaac accctttgct tcgatctcat caagtaattc tttcgtaaaa tgcaatccag
     6601 ctgttggcgc tgcagcagaa ccattttctt tcgcatagac agtttgatag cgatctgggt
     6661 cttccagacg ttctttgata tatggaggca atggcatttc tcccaatgat tcaagaatct
     6721 ctagaaaaat tccttcatac tcaaagtcaa tgatccgtcc gccgtgttcc aattcttctt
     6781 tcactactgc ttgcaatctg ccatcaccaa aagagattct tgtcccaact ttggcacgtt
     6841 tcgcaggttt gatcaatgtc tcccacgtat ctccttgtgt attcgttaga agaaggactt
     6901 ctaaatgcgc accagtatct ggtttttctc catataaacg agcaggcaga actcgtgtat
     6961 tattcataac tagcgcatca ccaggctgta actcttcaat gatttcatgg aaatgtttat
     7021 cttccatctc tccagttttc cgatccaaaa caagtaaacg agaatgatcg cggtctttta
     7081 atggtgtttg tgcgattaat tcttccggca agtcaaaatc aaaatcttct gttgttaaca
     7141 tatcgtcact cactttctat catgtgcttc atcattctat catgtttttc gttctgttgg
     7201 caaaatcagc actttttcac ttctattttt tgttttccta aataaaaaaa ctaccctatt
     7261 tgtatttttt cattgtttta ggcatactag taatagatag ttcttcattg aaaatagaaa
     7321 aagaggtggc accatgattg aatttcaaaa tgtgtcaaaa atttataaag gtggaaaagt
     7381 cgctgtagag gacgtcaatc tttcatttga aaagggtgag ttcatttgtt ttatcggtac
     7441 gagcggaagc ggaaaaacga cgacgatgcg gatgatcaac cgaatgactg atccaacaca
     7501 agggaaaatt ttgattgatg gaaaatccat ccaagaaatt gatccagtgg aattgcgacg
     7561 tcagatcggc tatgttatcc aaagtattgg attgatgcct cacatgacga tccgtgaaaa
     7621 tatcactctt gtccaaaaat tgttgaaagt cagtcaagaa gaacggaata aaacagctga
     7681 aaaaatgatt gatcttgtgg aattgccccg tgagatgtta gatcgctacc cacatgaatt
     7741 atctggtggt cagcagcagc gtattggagt cgttcgtgcc cttgcagcga atcaagatat
     7801 catcttgatg gacgagccat tcggtgcgct tgatccaatc actcgtgatt ccttgcaaga
     7861 ccttgtcaaa gatttacaag agcgtttggg aaaaacgatc gtatttgtaa cccatgacat
     7921 ggatgaagc
//
LOCUS       DAZUMV010000124         7927 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000124 DAZUMV010000000
VERSION     DAZUMV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.125"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG8FK_002351"
     CDS             414..947
                     /locus_tag="ACG8FK_002351"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS5097914.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1437..1961)
                     /locus_tag="ACG8FK_002352"
     CDS             complement(1437..1961)
                     /locus_tag="ACG8FK_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS5097915.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="ACG8FK_002353"
     CDS             complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="ACG8FK_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS5097916.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2550..3530)
                     /gene="pta"
                     /locus_tag="ACG8FK_002354"
     CDS             complement(2550..3530)
                     /gene="pta"
                     /locus_tag="ACG8FK_002354"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS5097917.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3683..4363)
                     /locus_tag="ACG8FK_002355"
     CDS             complement(3683..4363)
                     /locus_tag="ACG8FK_002355"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5097918.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4489..5358
                     /locus_tag="ACG8FK_002356"
     CDS             4489..5358
                     /locus_tag="ACG8FK_002356"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5097919.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5384..5899
                     /locus_tag="ACG8FK_002357"
     CDS             5384..5899
                     /locus_tag="ACG8FK_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS5097920.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5946..6509)
                     /locus_tag="ACG8FK_002358"
     CDS             complement(5946..6509)
                     /locus_tag="ACG8FK_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5097921.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6564..6743)
                     /locus_tag="ACG8FK_002359"
     CDS             complement(6564..6743)
                     /locus_tag="ACG8FK_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097922.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6820..7329)
                     /locus_tag="ACG8FK_002360"
     CDS             complement(6820..7329)
                     /locus_tag="ACG8FK_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097923.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7425..7805)
                     /locus_tag="ACG8FK_002361"
     CDS             complement(7425..7805)
                     /locus_tag="ACG8FK_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5097924.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         2278 a         1637 c         1418 g         2594 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
     1261 aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
     1321 ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg
     1381 tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat
     1441 tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct
     1501 ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca
     1561 ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg
     1621 atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg
     1681 ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt
     1741 ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata
     1801 taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact
     1861 aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct
     1921 ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc
     1981 tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg
     2041 cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc
     2101 ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc
     2161 taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat
     2221 ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat
     2281 ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt
     2341 caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt
     2401 cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc
     2461 cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct
     2521 gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata
     2581 atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg
     2641 ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta
     2701 tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact
     2761 tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat
     2821 tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact
     2881 ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt
     2941 ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct
     3001 tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt
     3061 ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc
     3121 tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct
     3181 gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct
     3241 gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc
     3301 cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca
     3361 tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct
     3421 gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt
     3481 ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct
     3541 ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta
     3601 tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact
     3661 ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt
     3721 ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac
     3781 gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga
     3841 tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc
     3901 gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag
     3961 aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag
     4021 cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt
     4081 tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc
     4141 catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt
     4201 cataaggagt caattccaat gcttcataga tatgatacat atctggatgg atcttttggg
     4261 acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg
     4321 aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat
     4381 aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga
     4441 ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta
     4501 attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat
     4561 gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga
     4621 aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact
     4681 ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca
     4741 aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta
     4801 gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc
     4861 atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct
     4921 aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag
     4981 aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca
     5041 gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat
     5101 atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag
     5161 cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg
     5221 ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat
     5281 gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg
     5341 attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat
     5401 tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc
     5461 cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat
     5521 gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga
     5581 gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga
     5641 cttttattat attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata
     5701 tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg
     5761 agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc
     5821 tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta
     5881 ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca
     5941 tctttttaga tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg
     6001 acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca
     6061 cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat
     6121 cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat
     6181 tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc
     6241 tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa
     6301 gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga
     6361 taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc
     6421 ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga
     6481 gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg
     6541 cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa
     6601 taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt
     6661 tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa
     6721 ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac tctcttagct
     6781 tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg
     6841 acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag
     6901 aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc
     6961 tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt
     7021 ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc
     7081 ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac
     7141 tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc
     7201 ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt
     7261 gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat
     7321 ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat
     7381 gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct
     7441 aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct
     7501 ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg
     7561 tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc
     7621 gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag
     7681 tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt attaacataa
     7741 aaatcacgtg atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg
     7801 ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat
     7861 tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa
     7921 aaaaaca
//
LOCUS       DAZUMV010000125         7865 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000125 DAZUMV010000000
VERSION     DAZUMV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.126"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..319)
                     /locus_tag="ACG8FK_002362"
     CDS             complement(26..319)
                     /locus_tag="ACG8FK_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS5097925.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(347..1012)
                     /locus_tag="ACG8FK_002363"
     CDS             complement(347..1012)
                     /locus_tag="ACG8FK_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS5097926.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1152..1784)
                     /locus_tag="ACG8FK_002364"
     CDS             complement(1152..1784)
                     /locus_tag="ACG8FK_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5097927.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1791..2858)
                     /gene="liaS"
                     /locus_tag="ACG8FK_002365"
     CDS             complement(1791..2858)
                     /gene="liaS"
                     /locus_tag="ACG8FK_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS5097928.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2855..3586)
                     /gene="liaF"
                     /locus_tag="ACG8FK_002366"
     CDS             complement(2855..3586)
                     /gene="liaF"
                     /locus_tag="ACG8FK_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS5097929.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3754..4233)
                     /gene="greA"
                     /locus_tag="ACG8FK_002367"
     CDS             complement(3754..4233)
                     /gene="greA"
                     /locus_tag="ACG8FK_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS5097930.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="ACG8FK_002368"
     CDS             complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="ACG8FK_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS5097931.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5709..6869
                     /locus_tag="ACG8FK_002369"
     CDS             5709..6869
                     /locus_tag="ACG8FK_002369"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5097932.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6909..7856)
                     /locus_tag="ACG8FK_002370"
     CDS             complement(6909..7856)
                     /locus_tag="ACG8FK_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5097933.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
BASE COUNT         2328 a         1665 c         1228 g         2644 t
ORIGIN      
        1 aaaacttgtg tcaccctctc tttttttatt gttttttaaa gtgataggtt ggctcctcca
       61 ggttgggatc ataatcaaca agtaaatcat atcctttaaa gaaccattca tctgcttcct
      121 cgataaaaaa aagtgtccca tccatttcca ctttctttac aggatgttct ggctgttcaa
      181 ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact tttccaaaaa
      241 aacgtattcc gtaagtttct ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg
      301 gcgttaccgt taattccatc ccaacaacct actttctctt ttctttctat tgattcatct
      361 tatcgtctag aatatccaca tcgcctgtct ctccaatcac cagtagatta tcattttcgc
      421 gaacatattc atctgctgca ggagaagcga taaccgtttg atctactctt cgtatggcga
      481 ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt
      541 taggattgga gactcggatt tctgctaaag aatatttatc tgacagctct aagtagtcta
      601 gaatattctt tgatactaag ttatgtgcaa tccgtatccc catgtctcgc tcagggtgta
      661 cgacacgatc tgctccgatc ttctctagta cacgggcatg gtactcattt tgggcttttg
      721 ctaaaatatt cggtacgccc atttccttta ccatcagagt aactaagata ctggcttgga
      781 tatcttctcc aatagcgaca attacatgat cgaaatttct tatacctaat gaacgaagtg
      841 tcatctcatc ctgtgcattt gccacaacag catgagtggc tatattcata tattcattga
      901 ctcgatcttc attactgtca atagccagta cttcttgtcc agcttcgatc agtgtacggc
      961 agatgcttcc gccaaatcgt ccaaggccta caattgcaaa attttgtttc accattttcc
     1021 tcctttagta tcttcttttc tcagtataac aaagcctttc caaaaacaaa agcaagcatc
     1081 attattgtaa aaaagccaag aaaaataata gttattttct cagcttttga ttatcctctt
     1141 tatcatcgtt tttatttcac caaaccatgt ttaaacgcat agatagctgc ttgagttcgg
     1201 tcttctacct ctaacttagc taatatattc gacacatgcg ttttaactgt tttcagtgtg
     1261 ataaacaatt catccgcgat ttcctgatta cttttccctt cagaaatcag catcaaaatt
     1321 tcattttctc gattcgttaa ttcttcatgc aatactggat catttcgccg actcattttt
     1381 tccatcattt ttgttgtcac ttcaggttcc aatactcgct cgccgcgttg cgtagccctt
     1441 atagcatcag ctatttcatg tgcggtagac gtcttcaaca aatatcctgc tgctcctgct
     1501 tcaattgcag gataaacttt ttcatcatca ataaaacttg tgacgataat gattttggct
     1561 tttggacaat cttttaagat tgcttttgtt gattcgatgc catccatttc ttccatgact
     1621 agatccatta aaatgacatc tggtcgaagt gccatcgcct tttcatagcc ttgacgtcca
     1681 ttttcagctt ctccaatcac ttcaatgtct tcttggatag ataaataaga agagacgcct
     1741 aagcgtacca tttcatggtc atctactaat aaaactttta tcatctttat ctatgcctcc
     1801 ttcatcaaag ggaccttgat ctccacgctc gtaccttgcc ctttaaaact aatgattttt
     1861 actgttccac caataccagc tactcgttct ttgatattgt tcaatccata acttcccgtt
     1921 ttggtttcat tcatattaaa tcctgtcccg tcgtcaatga tacgtaataa aagattgtta
     1981 tctattttat gcaaatagac ttctaattca ttagccttag catgtcttaa tgtgtttgat
     2041 aacaattctt gaacgattcg gaacaagtga tcctcaatgg aactagttaa ttttacatct
     2101 tctacatccc attttagcga aatctggatt ttattctgca attccttcaa taactgctca
     2161 atgccttgtt ttaaactttt ttcttctaaa ttgactggac gcagatgaag aagcagcgca
     2221 cgcatctcag actgggaagc gttgatgatc tctgccacca ttttcaactg cttacggaac
     2281 atttctggtg tctcactttt ttctgcttgc tccgtcaatg ccgacatcat catcatcgct
     2341 gcaaataatt gctgtgaaac tgaatcatga agctcacgag ctaatcgatg cctttctaat
     2401 tctaaaatct cttctttggt ttgtccatct acatattgag gacggctagt cataatctgt
     2461 aattcactgg atacttcgat cattttttct tttattttag caatttccat atcaatatct
     2521 ttgatgtaca gatcatccga cgcgcttggg atcggctgat gcaaaagttt ggattcataa
     2581 ttgccagaag aaagtgcacg aagcttttct tcgattttcc cgtattttgc cttttgaata
     2641 agtgataaca atagaaatac gatcaatcct acacctaatg aaattgccat caaatggaag
     2701 ataagcggaa cgtataataa acgcgcacgc agcaattctc cccaccaatg actttggtta
     2761 ctggcataaa aataagtgaa aagtgaaaat agaataataa ggaaagccgc aatccccgaa
     2821 taaacagcta gcattgcttt ggatattctt cccatcatat tcgtatcacc tcgacatctc
     2881 caaccaaggt attggtaatg atcttcaatc tgcgaggatt ttcatcataa tcactgctgc
     2941 agattttgat ctgttcgttt tttaaggaaa actgctcttc ttcaaacaag acatttccta
     3001 ctaatgtcgc atgctctaaa cgaatagcca ctcccagggg tactaagatc cgtgttctgc
     3061 ctatcccttt tcttacaatc acaatattat catcttttgg caaaagtgta ttacccaaat
     3121 cgatgatagt atcaccagaa atgatggcaa tgttgatatc atcccattca tagacgtcgt
     3181 ttccaatacg ttggttacca aataattgct gtcttttacg ctcattatta tgtgttttta
     3241 attgctctgt ttggaccatc atgatctgtt tttttcgcca aaaagcattt tttgtcaaat
     3301 ctattcctga aatctctaca cctttcagcc cgataaataa aacggccaat acaaccatca
     3361 tccataaagc aggactgttg actaagctaa aaaaaatgac caagctccct aagatcaatt
     3421 gaaaattttg aaatttagta cgaggatatt tgcgcatagc tagatatata ttgaaaatac
     3481 cgaatattac taaaagcaaa agtccagtat tgtttacgat ttgccaaaca gcaaataaca
     3541 gcagcagtgc ttcgactacc acaaaaaaac gccaagaact attcatcgct ttttctccct
     3601 cctgacacat tctcatttca tcatacctat ttttttgact gttggctata ggactttaga
     3661 tgtaagatcg ttcagtgaaa cctgcaatac tcaaagagaa aagatctaga gtgcgtcatc
     3721 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
     3781 aactaacata tcgccacctg gtgtagcaat ggttacttca tcatttttac gtttgccaat
     3841 caatgcttgc gcgataggag aatcattgga aatctttcca gaaaacgggt ctgcttcagc
     3901 acttccgaca atcgtgtact cttcttcatc tccatcaggc aactcgatga acgtaactgt
     3961 tttaccgatc gatacctcat ctgcatctac gccgtcatta tcgatgattt gtgcaaaacg
     4021 gatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttagctga
     4081 ttcgtattcg gagttttcgg aaagatctcc gaaaccgcga gcgattttga ttcgttcaat
     4141 gatttctccg cgtttaactg tttttagttc ttctaattct ttttctaatt tttctttacc
     4201 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
     4261 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttctta
     4321 aaatgtaaat gtttttctaa aaatgatcaa tgactagccc ctgtttttct aagaattatt
     4381 tacatatttt tgcactagtt ccatatgttc ttcatacgtt ttagcgaaat aaacatttcc
     4441 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
     4501 gattgccttt tcgcttggat tgtcaaaagg accaggccca tagccggtat tggtatacaa
     4561 attgtatggt gaatctacag ctgtatcttc atacgtcacc aattctttat gttctcctaa
     4621 tgcatataaa acagagatat ctgactgaag tggcatattt gccttgattc ggttaaagaa
     4681 gacttgtgca atatttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
     4741 caaagttaaa acttcttgta cagtcaaatt tttctgtttg atagctgaat aatagttttg
     4801 catgaccgta tttgtcttgt tcaccatctc aatcacaaga tccttaagcg aagtactcgt
     4861 gtagtaatca taagtagctg ggaataagta gccttccaaa cggtattttg tgttctcagc
     4921 tttagaggca ctttccaaca attctgggaa tttttgatac aactcgttaa agaaagtctc
     4981 atcatttact aaatctaaaa attctttctt gtctttacct gttaccttcg ctacccgctc
     5041 agcaatctga tcgatgtcgt agccttctgg aatggcaatt ttcccatctg caacttttgt
     5101 tggctcagat gtgccgcctt cttgcaactg cttgccaatc tcatcaagag tcatattagg
     5161 cgctaactga taataaccag cttgaaagcc agttaagttt ttaaattttg tataataatt
     5221 gaaaacaatc ccacttttaa taatattgtc tttctccaaa atttcgccaa tctgcttatt
     5281 agaagagccg tttgggattt ccacttggac taattggtcg tccgatttat ctaaaggctt
     5341 caacccgcta tctacataac gatatacggt aaacccgaga aatccgccaa taatcaataa
     5401 tgtaagagcg atcactaaaa tgatcttcct cacgatctta tcttcttttt tacgcagatt
     5461 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tgtctttgct
     5521 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcatcttc gtaactctgt
     5581 ttattataca tgaaatttct actgccgtga attattttac caaatattat gtcaaaattg
     5641 taagtggcaa atgctctttt tgaggattac aatgaaaaat agttaaaaat ggatagaaag
     5701 gatttaatat ggcaaaagga aaacatcaaa aaaaacagca gatctctctt tcttttctcg
     5761 cagttttgtt gctggctgtc agcttctttt caacaggcat cattacagga tggcaattaa
     5821 caaaacaacc tcaaccacaa atttataaaa cggaacccgc acaacttgct tccaatttaa
     5881 catctaatga gcagatgctt gccagcaaaa ttgatcaatt actgcaaaaa tcagattata
     5941 tcggttcgat ttatgtacgc aaaaataatc gagtgatatt agaaaaagga tatgggtacg
     6001 caaatctacg cataaaagaa tcgaattctc cttctcttta ctatcaaatc ggttcgatcc
     6061 aaaaagcgat gactgcttta cttatcctaa aacaaattca atctggcaag ataacttttg
     6121 acacaaagct atctgaattt tatcctcaga ttcctggaag tcagcaaatc tcaatcaaaa
     6181 atatgcttta catgcgctct ggtcttcatc gaaccgcttc tccaaaaaaa cctatgtcag
     6241 atagtgagat cattcagttt gcccttcatc atttgaaatt tactggttat gatacgtatc
     6301 attatgaacc attaaattat acattgctta caggggtatt gatcaaacta atgaatcagc
     6361 cttatgaaac tcttttaaaa aaagaaatca ttcggccact ccatctagaa catacagatt
     6421 tttatgaaaa tgtaaaaaat tcccctcagc atgctgtatc ttaccaaatg tctgcaactg
     6481 acgattattc aaaagcattg gtcgagcctg aaacagacat ccgaaatgaa ttaggcacag
     6541 gaaatataag tatgtctgtt tatgacttga acaaattctt taccagtgtt ctgtctggcg
     6601 atattatacc aaaagaacta cttttctcat tgtggcaaaa taatggtgat aaacatcctt
     6661 acagcggcgg tgtttacagt ggaaatgatt atatccttgc tcagggaaat atcaatcgat
     6721 tccatgcagt agcggcgatg aaaaaagaca caaaagatgc aatcgtcatg gaaagtaata
     6781 tccaatctga taaaaacatc aaattgccgg ccactgattt aagaaatcag atttacgaac
     6841 tgatggaagg tgttaattta aaaagctaag tcgagacaaa aaaaccagca ggatgctggt
     6901 tttttgtttc aatcgatttc tgtctttaaa taacgttcca ttccgttcaa tatatcaaat
     6961 ttgggttgat aacctaaaga acggatcttt gaaatatccg ctaaagaatc acgaatatcc
     7021 cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg atcaattgaa
     7081 tgaatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg ctgtcctaaa
     7141 gctttttctt ccttagcaac aagtaaaagt gcttgtataa catcttctat ataaacgaag
     7201 tctctagatt ggctaccatc accaaataaa gtaaattctg ttttttctcc tgccaattgt
     7261 tttttgtatc ggtccactag aatcgaaata actccgctat atggagagtt aggattttga
     7321 tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata taaatgacag
     7381 taatccaaaa catattgttc tgatgcaaac ttatcgatgg catatggtgt taaaggacgg
     7441 atgaccgatt cttctttttt tggcaacgtt ggttcatcgc cataaactgc agctgatgaa
     7501 ctaaatacca atcgttttaa accagattga tattttcgta ccaattcaag caataaaagc
     7561 gcactgtcaa aatttacacg atgagtttct agaggacgtt ctacagaatc tgccacacta
     7621 gctacagcag ctaaatgaaa aatataggca aactgatact gtttcatgat tctttccatc
     7681 aattgaggat ctgatacatc cccctcaatg aaagtcactt gtttctccat atccaagttt
     7741 tctttttttc ccattgataa atcgtcgact actacgattt tgttttcttt tcccaaataa
     7801 ttcgctagcg ttgaaccgat aaaaccagct ccaccagtaa tcaatatagt atccatcaaa
     7861 ttcac
//
LOCUS       DAZUMV010000126         7788 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000126 DAZUMV010000000
VERSION     DAZUMV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.127"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..751
                     /locus_tag="ACG8FK_002371"
     CDS             50..751
                     /locus_tag="ACG8FK_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5097934.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1323..2339)
                     /gene="tsaD"
                     /locus_tag="ACG8FK_002372"
     CDS             complement(1323..2339)
                     /gene="tsaD"
                     /locus_tag="ACG8FK_002372"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS5097935.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2336..2800)
                     /gene="rimI"
                     /locus_tag="ACG8FK_002373"
     CDS             complement(2336..2800)
                     /gene="rimI"
                     /locus_tag="ACG8FK_002373"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5097936.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2807..3349)
                     /gene="rimI"
                     /locus_tag="ACG8FK_002374"
     CDS             complement(2807..3349)
                     /gene="rimI"
                     /locus_tag="ACG8FK_002374"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5097937.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3333..4055)
                     /gene="tsaB"
                     /locus_tag="ACG8FK_002375"
     CDS             complement(3333..4055)
                     /gene="tsaB"
                     /locus_tag="ACG8FK_002375"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS5097938.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4246..5226)
                     /locus_tag="ACG8FK_002376"
     CDS             complement(4246..5226)
                     /locus_tag="ACG8FK_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5097939.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5250..6734)
                     /gene="galT"
                     /locus_tag="ACG8FK_002377"
     CDS             complement(5250..6734)
                     /gene="galT"
                     /locus_tag="ACG8FK_002377"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS5097940.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6746..7735)
                     /gene="galE"
                     /locus_tag="ACG8FK_002378"
     CDS             complement(6746..7735)
                     /gene="galE"
                     /locus_tag="ACG8FK_002378"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS5097941.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2171 a         1686 c         1381 g         2550 t
ORIGIN      
        1 taaaaaaagt gacaataaat atagtgaaca ataaggagag gactttttaa tgcgtgttaa
       61 taaaatcgat tttcactttt caaaactcag atatcaacct gattttgaac atttctcgga
      121 tgaagagttc caacagatca aggaacatac tgttcttcgt tcttataaaa aaggacagat
      181 ccttttcgac gatggtgatc ccagacaacg gttgtatatc ttgaccaaag gactcgttcg
      241 tttagaacgt tatgatgaaa gtgcaactta ttactactat gactatgtga atacaaataa
      301 cctttttcct atgtcaggaa tgtttggaga agagacctac acttatacag ctcaagcgat
      361 gactgatatt gaaacattct accttcctgt ttcctactat gaaaaattag cacgaaaaaa
      421 tcccgaacag ttgatctata tgatacaaaa aatttctaaa gtagttgaaa tacatgaatt
      481 gcgtatccaa atcggattga cctctagcgc ctttgaccgg gtaaaacaaa atttattcat
      541 tttgaaagaa gaattgggcg taccaacaaa agatggtcat atcaccattc cttatccgat
      601 caccttaaaa gaattagcag tgaatagcgg cacgacacgt gaaactgctg gacaagtaat
      661 caaacaacta cgtgattccg gactgttaga ttatgacaaa aagcgtttta ctttctgtaa
      721 gacagctact actccagcta tgagagaata acgcgaacaa aaataagcag tgaccctgat
      781 atttatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta
      841 gatcactggg caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt
      901 ttgcaaaaaa tactgataga ttaactagag gaagcgtctt gtcctcatcc gacctagatt
      961 attcgatatt cctcgacaat aaacgattaa tcagtgaggg caatcagagg aacggataat
     1021 gatgaataag ctgtaaaaaa tagggctttg acaaaagcgt gtcacagccc ttatttcgat
     1081 taaacggtgt gtacctgtca actcttcggt cacaagtcat aatgttcata aacatgagga
     1141 gctgtttttt gaaattgttc cttgtgaatc agagttaaac gacaggctta cgaactcttg
     1201 attaaacggc gttcaaaagc ttgcatacgc ggtaataaga acagccaggt caaaaacatg
     1261 agaagccgtt tttcctgggc tgttcttatt acttgatgca gggcagattt ttcacaacct
     1321 ctttattcag ataaaaccaa gcttggatca gcattcaact gatagctagc aaaatttctt
     1381 ttttgcattt ccacatgtgc tgctgctcca atcattgcag cattatctcc acaaagtcga
     1441 agagggggaa tcactagttc tacctcaggg agtttagttg ccaaagcatc ctgcaatcct
     1501 tcacgcaacc cttgattggc cgctacacct ccagctacaa tcagttgctt gactggataa
     1561 ttttgacaag ctcgaagtgt tttagtaatc aatacatcga tcacacttgc ttgaaagcta
     1621 gctgctaaat catttttgtc tagatcttct ccacgctgtt gtgcattatg caccaaattg
     1681 ataaatgcac tttttaatcc gctaaaactg aaatcataat tatcttcatg gatcatcgct
     1741 cgtggaaaat ggtaattatc ttttccctga tgagctagct gatcgatttc ttttccgctt
     1801 gggtaagaaa gacctaatac tcttccaact ttgtcataag cttctcctgc tgcgtcatct
     1861 cttgtctccc caatgatttc atatgatcca tcttcttgca tatagactaa ttctgtatgt
     1921 cctccgctaa caagaagtgc catcaatgga aactggaatg gtttaaccag acgggctgca
     1981 tagatatgcc ctgccatatg gtttacagga ataagcggca attggtgtgc ccaagcaaaa
     2041 gccttagctg cagaaatacc aatcagcaac gagccgacaa gtcctggtcc ataagtgaca
     2101 gcgactgccg ataaatcttc tgccgacaca ccagcttcta ccagtgcatc ttccaagcat
     2161 aaggtgattt gttctacgtg atggcggcta gctacttcag gaactacgcc accaaaacgt
     2221 ttatgactgt ttatttgaga agcaacgatg ttagataaga tctctgttcc atttctaacg
     2281 accgctacac tcgtttcatc acaacttgat tcaatcgcca gaatcaattc ttcattcatt
     2341 tcatcgtctc cgtttttaac tttctgctca taatgatcgc atcttccgtc ggattgcgat
     2401 aatatgattt ccgtttacct aatatacgaa acttttctga ttcataaagt tttcttgctg
     2461 cttcattaga tagacgtact tctaaaaaaa catgcagcga atcttttatt tgttccgtaa
     2521 tcagtaattg taacaaatgc cgtgcatgcc cttttccttg ttctgaaact gcaacagcaa
     2581 tatttgtgat ctcgacttca tctattactt tcgagtatcc aataaagcca cagatctggt
     2641 tatcttcaac taaaagcaga taatcagagt gaggctgttg gatgtctgcc aaaaactgca
     2701 ttttcgtcca aggtgaacca tgtggataag cattttcgct aatcatccat acgcattctg
     2761 ccaagtacgc ttcatcaaaa tctttttttc tgcaaatcaa ctaatctcac tcattttcta
     2821 aaagttttat catatccaac gcatcttctt ttgtttcagt gtaatatctt tttttgatcg
     2881 ttgttgcttg aaaacctaac ttccgataaa gtctttgtgc atcttgatta ctcatacgca
     2941 cttctaatga catcgtctca cagcgattca ttatagcaaa gttctcgatt tcgtcaataa
     3001 gcagcgaacc gattcgttta cgctgatatg ccggtcgaac ggcaatattc gtgatatggc
     3061 aatctaaacc gaacgctctt gaaccgataa aaccaatcat tcttccatta tcaaagatac
     3121 ctagatatag atgaggaagc ggtgacttca attctgatat aaaggcactt tttgtccatg
     3181 gcaattcgcc attatagact tctcgttcga catccagcag ctctttgata tcttctgtag
     3241 tcaattctct tagtacgtaa cgacgatcga tttgttcgat tgttttttct gtataaatca
     3301 ttttatcttg aaaaaagctt ctggcatacg ctctaaattt tttcaacata actttcgtct
     3361 ccaggtgtgt gcgtctccaa ccatttttct tctgcttcta cccgttttaa atagcgagga
     3421 agaaaagctt gtttctctgc aactggttca gctaaagcac ctaattgagc cacagttaca
     3481 ccacttggaa tatcccatgc tggaactgta ttaatcatcg cactaggcaa tatttccttt
     3541 atttcataga agaatttgtg acaatcactc ccgacgaaat aaatagcttc attcagcttt
     3601 tgtagttgtg tgaacaaatc agaagcagaa atatgtacat cttcaagtac aatttctaat
     3661 ctattttctt tccagcggta tgccccagcg tatacatttt ttcttcgagc atcaaacatg
     3721 ggcacaataa ttttatccac accgatgcaa ttagccgcga tggttttcag actagatact
     3781 cctactagct ctttatctaa tgtataagca atcgtttttg ctgtcgttac acctatacga
     3841 agccctgtat aagaaccggg accatcagaa accgcgattc ggtcaatgtc ttttggactt
     3901 atttgaagat ctgcaaacaa ttggtcgatt gcaggcatca gtgttacact gtggttgcgt
     3961 ttgatatttg tttgtatttg tccaagaatt tgttcgtctt ctaccacacc gatcgccaaa
     4021 gtttggtttg cagtatctat tcctaatgta atcatatatt tgatcctttc tgctatgaca
     4081 ttctcgcctt ccattgtagc atactttttt attctgagga tgaaaatacc aaaggcaaaa
     4141 ctagtacaat gatacatgat tatctgctgg cgggcaaatt ttgccccttc ctgaaacaca
     4201 aaaaagctgc ggtatcactt catttcccca cagcttttcc atcttttatt tcgttgattc
     4261 tcgaataatc aactctgttt ccacaataat ctttcttgaa accggtgctt tggaattact
     4321 taattgaaga atcgtctcca ctgctagctc tcccatccat tcggtataaa cttttacagt
     4381 agaaagagag ggcgttacat atttcgctac actgacatca ttgaaaccca tcacagaaat
     4441 atcttctggt accactaaac ctttttcttg aatcgctttc agtgcgccaa tcgctaatgc
     4501 atcattagct gcaaaaagcg catcaggaac atgcggattc gtttttaaat actgcttcac
     4561 tgcttgatat cctgactcga cagaaaaatc tgcttcgata attggacctt cgtcgtagcc
     4621 tttttctgcc aaaacttgtt gaaatgccat aattctggga tcttttagcc gctgatggtt
     4681 tttcttcgta tattctgttc cagcaagcat agcgatatgt tgcgctttat gggagacaaa
     4741 atgttcaatg actttttgaa cacttgactt aaagtcgaca actagagagt cgattccttg
     4801 agaagtgccg tcagaatcta ccaaaagcaa ggcattttgg gatcctttca tttctaataa
     4861 ctgagcttcg tcgaattttc ctagtgcgat cgttccatct gtcggaagat cactaatgga
     4921 atcccacgtt tctttcaata aagtaacgtt tgcttcttct gccttttttt cgattcctag
     4981 acgaatagat aaataataaa gatcttcaag ctcttccgta tcgtcgtacc actggatcag
     5041 gcgaagcgtt tgcttagtat ttgctgcatt tttacgatgt ttcgtataat ttaagcgttc
     5101 tgctgcttca aaaatttttt gctttgtggc taagccaacg gaaagatcag gatcatagtt
     5161 gagaactctt gaaacagtag caggagaaac tcctgccaac tgcgcaatgt ctttaattgt
     5221 tgccatttgt ctctcctcct tttttacgtt tataaaattg cagtaaatcg ttcaaacgct
     5281 gcctgtcctt tttcatctcg cttgaatact ccagcgtctt ccagtacacg tgcaaaaacc
     5341 tgacctacag atttttgtac gacatcggtg accgtatctt tcgtcaaatc aggatgttct
     5401 ttttttaatt gttctgccca cggacgatga tattcagcca tctggttttc ttctcctaat
     5461 aagaaatgct ctacttctac tagttctggt ttcaaacgag gtggtaaaac agcaagcccc
     5521 attacttcga tcaacccaat gttctccttt ttgatatgtt ggacatcttt atgtggatga
     5581 aaaatcccat caggatgttc ttctgaaaca ttgttgtctc gaaggacaag gtccaactca
     5641 aagtaattgt cccttcttcg tgcaattggc gtaatcgtat ggtgtggtgt accatctgct
     5701 gaaaaagctc tgatagatac tgtttcatcc gaatatgctt tccacttttc caatactgag
     5761 ttagctgctt caatcaacaa ttttttctct gttgattgca aacggatcac agacatcggc
     5821 catttcacga ctcctgcgat gacttgcgga taatctgcta gtttgataag tttttctatc
     5881 ggtgcttttg ccataggaaa ttcatgacgt cctgcctgat aatgatcatg agaaagaatc
     5941 gatcctccaa caatcggcaa gtcagcattc gaaccaacaa agtaatgagg caagacttca
     6001 gtaatccgaa gcagtctagc aaatgtttcc tttgtgattt tcatcgggcg gtgttgttct
     6061 gataaaatga tcgaatgttc attgtaataa gcataaggag aatattggaa tccccagctt
     6121 tcaccgtcta gattcattcg aataatccgg tgattggtgc gggctggata gttcaagcga
     6181 cctctatatc cttcgttttc catgcaaagc atgcaggtag gatagtctac ttttaccgcc
     6241 tcgcgttctg ctgcgatttg ttttggatct ttttctggct tagataaatt gattgtgatt
     6301 tccagttcac catactccga ctctgccgga aatttgatgt tttttgcgat ggctcttgtt
     6361 ttgatgtagt catttttctt acttaaatta aagaaatact cagttgcttc ctctgggctt
     6421 ttcgaatagt gctgtgcaaa gaatgcattg acgactgaag gtggcggtgt gagaaaatcc
     6481 attaattgcg cttcaaaaat ctcttgttct gctggcaatt ctgaaagaac accattttct
     6541 cttgccttag cgacaagctg atccaataag tctacagatt cttccttaac tggtcttact
     6601 tcaatgcttt ctagagcatc ttctcctacc atccctaaaa gacgattctg taaatataag
     6661 cggtccattt ccatccatcc gccagcttcg atcgcaagtg tggcaaaatc acaaatcgtt
     6721 tgactgattg acatatctca ttcccctatt cttcataacc atttggatgg ctgacatgcc
     6781 aatcccatgc tgttttgata attgctttga tatctgtgta ttcaggtttc caacccagaa
     6841 cctctctcgc tttttcactt gaagcgacta atcggctagg atctccagct cttcttggtg
     6901 ctatttctgc tggaatttct tttcctgtca cttctctagc tgcttctagc atttccttga
     6961 ctgagtatcc tttgctgctg cctaaattga agaagttgct ttcatttcct tctttcaagt
     7021 attccaaagc taaaatatgt gcagcaatca aatcttctac ttgaacataa tctctgatac
     7081 atgttccatc tggtgtatca taatcgtctc cataaacagc caatgctttt ctttgaccaa
     7141 gagctacttg taaaatgatt gggacaagat gcgtttctgg tgtatgatct tctccgatag
     7201 atgcatctgc ttttgctccg gctacattga agtagcgcaa agcgacatag cgcatgccat
     7261 atgcttggtc acaccacttc atcatttttt ccatcatcaa tttactttca ccatacggat
     7321 ttttaggatt agctggtgta tcttctgtga ttggagactc ttttggttct ccgtaagttg
     7381 ctgcagtaga agagaacacg attttattca cattgaactc gtgcatgact tcaagtaaga
     7441 tttgcatacc gtatacgttg ttgttgaaat acatcaatgg tttttcgaca gattctccta
     7501 ctaaagagct tgcagcaaaa tggattactc cttcaatcgg ttccttttca aatacggaac
     7561 gtagaaattc tttatcccgg atgtctcctt cataaaaatg agcatctgga tgtactgctt
     7621 gtttgtgacc tgtgagcaaa ttatctacca cagctacttg ataccccttt tgaactagct
     7681 ggtctacagc gtgcgaaccg atataaccgg ctccgcctag tactaaaatt gtcataaaaa
     7741 acactccttt gattagtaaa attgtgtttg ttgattatat ataattta
//
LOCUS       DAZUMV010000127         7623 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000127 DAZUMV010000000
VERSION     DAZUMV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.128"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..712)
                     /locus_tag="ACG8FK_002379"
                     /pseudo
     CDS             complement(14..712)
                     /locus_tag="ACG8FK_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            791..967
                     /locus_tag="ACG8FK_002380"
                     /pseudo
     CDS             791..967
                     /locus_tag="ACG8FK_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="ACG8FK_002381"
     CDS             complement(1143..1712)
                     /locus_tag="ACG8FK_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5097942.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITDVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ACG8FK_002382"
     CDS             complement(1786..2109)
                     /locus_tag="ACG8FK_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097943.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ACG8FK_002383"
     CDS             complement(2263..3075)
                     /locus_tag="ACG8FK_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097944.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3402..4277
                     /locus_tag="ACG8FK_002384"
     CDS             3402..4277
                     /locus_tag="ACG8FK_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5097945.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4291..4614
                     /locus_tag="ACG8FK_002385"
     CDS             4291..4614
                     /locus_tag="ACG8FK_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097946.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4611..5363
                     /locus_tag="ACG8FK_002386"
     CDS             4611..5363
                     /locus_tag="ACG8FK_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS5097947.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ACG8FK_002387"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ACG8FK_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1510 c         1371 g         2470 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacat cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aac
//
LOCUS       DAZUMV010000128         7591 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000128 DAZUMV010000000
VERSION     DAZUMV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.129"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(129..419)
                     /locus_tag="ACG8FK_002388"
     CDS             complement(129..419)
                     /locus_tag="ACG8FK_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097948.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(537..797)
                     /locus_tag="ACG8FK_002389"
     CDS             complement(537..797)
                     /locus_tag="ACG8FK_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097949.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(934..1347)
                     /locus_tag="ACG8FK_002390"
     CDS             complement(934..1347)
                     /locus_tag="ACG8FK_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS5097950.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1803..2288)
                     /locus_tag="ACG8FK_002391"
     CDS             complement(1803..2288)
                     /locus_tag="ACG8FK_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097951.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2427..2642)
                     /locus_tag="ACG8FK_002392"
     CDS             complement(2427..2642)
                     /locus_tag="ACG8FK_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS5097952.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2866..3666
                     /locus_tag="ACG8FK_002393"
     CDS             2866..3666
                     /locus_tag="ACG8FK_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5097953.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3685..5553
                     /locus_tag="ACG8FK_002394"
     CDS             3685..5553
                     /locus_tag="ACG8FK_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5097954.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPTNRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQNTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5546..6037
                     /locus_tag="ACG8FK_002395"
     CDS             5546..6037
                     /locus_tag="ACG8FK_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS5097955.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6025..6579
                     /locus_tag="ACG8FK_002396"
     CDS             6025..6579
                     /locus_tag="ACG8FK_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS5097956.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6597..>7591
                     /locus_tag="ACG8FK_002397"
     CDS             6597..>7591
                     /locus_tag="ACG8FK_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS5097957.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1441 g         2230 t
ORIGIN      
        1 atgaataaca taataagaaa gtaaagtgga ttgatttgag atctttttat acttcaaaaa
       61 agataacacc caactttcaa aaatatgttt tggaagttgg gacaaacgag ttaatacatt
      121 ttaatacatt attttttatt caaaaaaaat agcacaatgg caaagatcgt aagagagatc
      181 ataagcccca atgaatgact aaaaagaaaa gcagaatcac atgccatatc atcccatatt
      241 tcttcaagaa aataaataag cgggaaaaag ggaaggaata gtagaacatc gatccagcac
      301 catttttttc ttcttttgaa ataacataaa acaataaaca tgaacgtgca gggaagtaaa
      361 aacaaagctt tactcataac taacaaaata ccagctcctt ttattttttt ctcccccaca
      421 ttcaaagagt aacaatggta tgtacttaat aatagaaaaa tggatcaact tgtatgaata
      481 acagctgaaa agatctgcac caaataaaaa acttccagcc aaaactgact ggaagtttat
      541 ttttttctga aatattctaa cacaaatatg aaaaagatgg ccgaagctaa taagccgatt
      601 tcgtagcaaa acaatgagat accagagtcg taataataaa atcctctaat cttatggata
      661 aaatagatca tcggacaagc agataagagt aagtatttgt taggcctttt gaataagtaa
      721 aagaaagcac tggtcattat gataaatggt atccaaaaca aaagtttact taagacaatc
      781 aaatgatcaa ctcccatatt ttttaccaag agagatcata acatctttat cgtaaaaaca
      841 aaatattttt ttgctcattt tgaaaattta aacatactcg ttcttatcca aatatataaa
      901 aatcgcccct cattcatcga gaggcgataa gttctaattt aactcaattt tttttgtttc
      961 ttttggctga taagctgttt taggtaatac caatttcagc acaccgtttt taaaggttcc
     1021 agtgattttt tcttcatcaa cattaggaag atagaatgaa cgtttgtaag agaccgaatg
     1081 tctttctttc cgtacatagt tgccatcttc tttatcttct gtatgggact catgattggc
     1141 ggagatcgtc aaagtattat ctgaatattc aactgtcgtg tcttctttgt cacagccagg
     1201 catatcagca gttagtttgt attctttttc attttcgact aaatctactt ttggataact
     1261 tgaaacacct aaaaaatcat tgaatgctgg actaaacacg tcattgaaat cagggaacat
     1321 gtcgcgaatg ctaggtacat ttgccatttt tgatcatcct ttcaataagt attttttatt
     1381 tatccctatt tacgtaataa ctctattttg aaaaaaatgc aagtataatg gttagctttg
     1441 tgaatatttc atctaatacg atacaaaggc tatctttcta atttgaaaaa gggatcatta
     1501 aaataataag aagaaaatat gtcaaaaggt aaaaaagtaa attcttctat ccaaatatag
     1561 aaaaattgaa tctagaaaac cgcccgacaa taaatttcat taactcagtt gaccgaaaat
     1621 gaagtagata ccaaaaattt atcaagagag caagtagaga agtgagtata ttcataagag
     1681 aaatagctgt aacgaattat taggttaatg caaatgctta tttatactcc gtataacttt
     1741 ctcagaaaaa atgataccaa taatgacttg ctagtactgt aaatccgtat aatatcatct
     1801 aatcaaaatg gtgttccatt tgcatctgtc atcgtaatcg atccttcggg tgacactcga
     1861 taaaaaccaa cgcttccaga tcctccttgg gaacggattt gtttgctgga agctgtgaac
     1921 aaataatctc gatcaatttt ttgataaaag ctaataacaa tatcgtcact ttgtaattcg
     1981 gaacggtggg caataagaaa tagaagtgct ttgtcagagt tttctaatgg aagagaaggt
     2041 acagtgcttt cttgcatttc agatgttgaa gaaatgctaa acgactgaat acttgattgg
     2101 ctatgttgcg aatctacatt accagtagaa gtattagtaa tggaagtagt agaagtactt
     2161 ttagttgaac caacctcagt tgttgaaatg gctgctgaag attctttaac agaattcttt
     2221 gttttatatc cacaggcaga aaaaagtatt acagttccca acaagcctgc gtaaatccct
     2281 ttatgcatga tagctcctcc taaaatagat tatctttatt cagtatatca cttaaacaaa
     2341 gaaaagcaga tggtttaaaa tgaaaaaacc ttcccaataa agggaaggtt catgaagata
     2401 ataacttttt taataattat agctttctaa tatctttcat caacttttct agtaatcatt
     2461 gggaaaaatt tcaagcagta gatcgcacaa atacctacga cgatataaat gatcttcgca
     2521 aaaatagttg ttgaaccacc agcaatcata gctactaaat caagttcgaa caagcctacc
     2581 aacagccagt tcaaccctcc cacaatcaat aaagttaatg caatagcatc taaagttttc
     2641 attagaattc tcctttctta aatgataaaa caattatata tcaaaaagag aaacattgct
     2701 tatataacgc tcaggaattt tagcaatcta tgcaaaaaga tgaaaatttt ttttgctctt
     2761 ccattagtac acatttttta gctgaagctg taaatttcat gtcttaattt tttttagtga
     2821 gcttttatac tggatttgta agaaaaaaga aggagaggat cacatatgtc ggattggttg
     2881 gatattgcag gaaaagtagt aattgtaact ggaggttcct caggaattgg aagaagtatc
     2941 gtcgaaaatt tgttgaagca gaatgcacaa gtagcgaatt ttgatgtaac tgaatgccgt
     3001 atacagcatg aaaacctact atctttaaaa gtcgatgtgt cttctaaaac ggacatagaa
     3061 gaagggatat ataaagtaat gaaacatttc aagacgatcg atggtctagt caataatgca
     3121 ggaataaata ttccttctct tctaatagat agaaatcatc caaaaagcaa gtatgaatta
     3181 tcagaacaag tttttgataa gatgatcgct gtcaatcaaa aaagtgtata tttgatgagc
     3241 caagcagttg gaagaatctt ggtgcaaaag ggatcaggag tgatcgtgaa tctttcttct
     3301 gaatcaggat tggaaggatc agaaggacag agctgttatg ctgctacaaa agcagcgatg
     3361 aacagtttta ctcgttcttg ggcaaaagag ttaggaaaga ggaatgttcg tgtggtaggt
     3421 gttgcaccag ggatactgga agaaaccggt ttgagaacac aagaatatga agaagctctt
     3481 tcttatacga gaggtataag cgtcgaacag ctgagaaacg gttacagtcg aacaagtacg
     3541 atccctctag gaaggagtgg taagctgcaa gaagtggcgg atctagtctg ctattatctt
     3601 tcagaaagat caagttatat cactggagtc acgactaata tttcaggagg taaaacaaga
     3661 ggataaagga taggggggac gcacgtgacg ctagtaaaca gatggtacca attattgaaa
     3721 ttactagtca ctcacaagaa agtttcaatc caggaagtgc aaaaagaaat caaaaccagt
     3781 aatcaaacca taaaaaaaga tattggggag ttgaatgaag aaattgccgg tattgcaaaa
     3841 atcatccaaa aaaacaaaca ttttgaactt gaaatttatg attttgattt ttttgatgag
     3901 atcatgcaag gaaaattgaa aacagccagt gatttcaatt cggcatccaa aagaatcgct
     3961 tatttactaa ataggctgat cgaaacagag gatttcttac gtatggatga tttgtctgaa
     4021 gaagtcggtg tcagcagagg aacagtggtc aaggatctaa agacattaaa agagatgatc
     4081 aatgattttg atgtaaagat tacaggcaca ccaaataaag gactaagaat aataggagat
     4141 gagctagaat tacgactact tttattttat tttgtttctc cctacttttc agagacgaaa
     4201 acagaacagt ttctagaaga aaaacaagca atcaaacatt ttcagaagaa gacgaatgct
     4261 agtaaacaag aaatcaccct tttaacgaaa gttatcggta tttctttgaa tagaatctct
     4321 agtaactatc tgttgaagaa gcccaccaat cgttacagtg ggtgttttga atatatccaa
     4381 gaattcaatg aactgatcaa tcagttggaa gaaatctatg aactaacttt gagccaatat
     4441 gaaaaagatt ttatcagttt tcctttgaac atcatcaaca caagtataac gatgataagg
     4501 aaagctaact ctgatttgcg cttatatttc gaccccatga tgatgcgtat tcgacgatta
     4561 gatctcattg atctggatga gtccttctta tatcaagaga tgaaacagca cctctcttta
     4621 ttgataaatc gggtaatttt tcgttatcga ttgactgatt tattttatgg tgagatagaa
     4681 aaaaagtatc ctttttctta ccaaatcgct agcgaatgca tacaagcatt agaagctatc
     4741 ttgcaacata agatctctaa agtagagatc agctacctaa cgttgtattt ggagttagca
     4801 tttcgatatc aaaaagagca tttttcctct aaaaaagtag ccattgttca tacgacagga
     4861 aaaggaacag cattaatgat ccaaagacaa atcaaaagga tattggggag ggatatacaa
     4921 ataacacatg tttcagaaga acaatatgaa aaagtagatc taaacaaata tttcgctgtt
     4981 tttacaacga ttccgttaaa aaatgtaaat gaagagacac cggtgatcca tttgactaat
     5041 ctttttgatg actcttggtt aagggcacaa tggcaaaaag caaaaaaaaa gcgattagcc
     5101 gggtttcatt ctactgtttt tttctatcaa atatggaaag aaaaaaagac gtatcaagaa
     5161 tatttaaaca aattagtcgc tcctttgatc agaggctatt atgtggatga agaatttgaa
     5221 aaaaggatct tgaaaagaga atatcagcaa aacacatttg ttaacaaaga atttgctttt
     5281 cctcacacaa ccaaccagct atctcaacac attgttttat cagtagggct aataactgat
     5341 caaggtttag cagaaacagg aggaaatgga gttatttttt tatttgccat acctgaagaa
     5401 atgacggctg aagttgagaa tgaattattt gagctatacg atatcctatt tgaactcata
     5461 aaaatgggaa tagatgagtt tattgaatta gcaccgacac aagaatcatt tttaaaacta
     5521 atcaaaagta aaggagagag acttcatgaa tgatatgtat ctattcatag ttttcgcagc
     5581 ttcagcttat gtgctgcaag cagttatcag ttggaagcaa ctaaagcatt tcaatcaaac
     5641 atatagcgaa ctgcgaagaa aaggaaaagt agcgattggt aaacgacctg ggaaaatccg
     5701 ttcaggcacg attgttatgc tagctataga cgatcatgat caagttttag aagtcagaaa
     5761 aatgcaaggg gttacatctt tggcaaaatt tcattcatta cctgtctatg cgggtgaaga
     5821 tatccattac atcgatcgtt accatccctc agttcgagcg gaagatcgtt tgacacaagc
     5881 agctttagaa aatgccagag aagtatactt acgaatagag gcaggaaatg aaaaaagtga
     5941 acaaccttca ttgcctttta ttgctatgct caataatttt cggatatgga aaagtacgat
     6001 caaagaaaaa aggagtgaca aacgatggat atcataacaa aatttgctga gggatttatg
     6061 aggctgttcc aaacaggtgc agaaacattc ataagctgga tgacaggaat cgtaccagtt
     6121 gtcttgatgc tgatggttgc aatgaatacg ctgattgctt ttttaggtga agaccgagta
     6181 acgaaagtag ccaaattatc agcgaaaaat ccgcttactc gttaccttgt gttaccgttc
     6241 ttatctgctt ttatgttggg aaatcctatg tcatttacaa tggctcgttt ccttccagaa
     6301 tactacaaac caagctatta tgctgctcaa gctcaatttt gtcatacgag caatggtgta
     6361 ttcccccata ttaatcctgg agaattgttt gtatggatgg ggattgctca agggatcgaa
     6421 accttagggc taaattcgat ggagctggct atccgatact tgcttgtggg actgctgatg
     6481 aactttattg gtggttggat aactgatttt actactggat ttgtatgcag acagcaagga
     6541 atcgttctta gcaaaaaagt agagctttca gttgattaga gaaaggagag ttttaaatgt
     6601 atggtgatat taagatcgta agaggaaacg gcgggtatgg aggtcctttg attattaggc
     6661 cgacaaaaga taaacacaag ttcatctatg tcacaggtgg aggtgagagg ccaatgatcg
     6721 tagataaaat agaagaactg acaggtatgg agtctatcaa tgggttcaaa acatcaatac
     6781 ctgatagtga aatcgcgctt gcaatcgttg actgtggagg gactcttaga tgtggaattt
     6841 atcctaaaaa agggatacca accatcaata tcgtttccac aggaaaaagt ggcccattag
     6901 cacaatatat cactgaagat atttatgttt cagcagtagg gttagaacaa atacagccac
     6961 taatggaaac tgatgataac aaagaaacag taaatactgc atctccagat aaaaagtaca
     7021 aatatgatac ttctaaaaag ataaccgagc aaaaagcaga gcagcaaaac tggatagcaa
     7081 aaattggaat gggcgcggga aaagtagttg ctacattcaa tcaatcagca agagaagcag
     7141 tagatacgat gatccggaca gtgatccctt ttatggcttt cgtttccctt ttgattggtg
     7201 tgatacaagg ttctgggatc ggtaatgtat ttgctaacat tatggcacct ttagcaggaa
     7261 atgcagttgg attaacaatc attggcttta tttgttcact gccattttta tctcctttgc
     7321 ttggtccagg ggcagttatt agccaagtga ttggaacatt gatcggtgta gagattggga
     7381 aaggaaatat tcctccccaa ttagcattac cggcactatt tgctatcaat actcaaaatg
     7441 catgtgattt tattccagtc ggtctaggtt tggaagaagc aaaagctgag acagtagaag
     7501 taggtgtacc atcagttctt tattcaagat ttcttaacgg catcccacga gttatcattg
     7561 cttgggtagc aagttttgga ttatatagct g
//
LOCUS       DAZUMV010000129         6673 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000129 DAZUMV010000000
VERSION     DAZUMV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.131"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..661
                     /locus_tag="ACG8FK_002398"
     CDS             <1..661
                     /locus_tag="ACG8FK_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5097958.1"
                     /translation="LNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFV
                     YAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMG
                     IDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNS
                     GALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSAL
                     F"
     gene            673..1593
                     /locus_tag="ACG8FK_002399"
     CDS             673..1593
                     /locus_tag="ACG8FK_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5097959.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1634..3091
                     /locus_tag="ACG8FK_002400"
     CDS             1634..3091
                     /locus_tag="ACG8FK_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5097960.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3436..3636
                     /locus_tag="ACG8FK_002401"
                     /pseudo
     CDS             3436..3636
                     /locus_tag="ACG8FK_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            complement(3840..5159)
                     /locus_tag="ACG8FK_002402"
     CDS             complement(3840..5159)
                     /locus_tag="ACG8FK_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFS5097961.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            5389..5811
                     /gene="mgsA"
                     /locus_tag="ACG8FK_002403"
     CDS             5389..5811
                     /gene="mgsA"
                     /locus_tag="ACG8FK_002403"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS5097962.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      5998..6096
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            6134..6670
                     /locus_tag="ACG8FK_002404"
     CDS             6134..6670
                     /locus_tag="ACG8FK_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5097963.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         2093 a         1175 c         1364 g         2041 t
ORIGIN      
        1 tttgaatctg ctgatctcag tcgtatttgc aatcattatg agcgagttga gaaataaacg
       61 actcgttaaa gtttatcaaa cgatgtcctt actaccttat tttctatctt gggtcattat
      121 cagttacttt gtttacgctt ttttaagtcc tgacaaagga atattcaatc agtggatcac
      181 tggacatgga ggagaagctg tcaattggta taatgaaccg aaatattggc cgtttatctt
      241 ggtgttcatt ggtacatgga aagggattgg ttataacagc attatttact ttgcatctgt
      301 tatgggaatc gatccgactt attatgaagc tgctatggtt gatggagcta gcaaatggca
      361 gcagatcaaa catgtgacga taccacaact tattccacta atgacgattt taacgatttt
      421 agccgtagga aacattttcc gagctgattt tggcctgttt tataacattc ctaggaattc
      481 cggtgcatta tatgatgtga ctcaagtttt ggatacgtat atctacaatg gcctgacatc
      541 cacaggagat tttggtatga cggctgctgc aggactttac caatcagttg tcggctttgt
      601 cttgttgatg atcacaaata cgatcgcccg acgttttgat agcgattctg cattattcta
      661 aggaggaaaa aaatgaaaaa aaagaaagtg gagagagtcc aagtccgctc tttcaataag
      721 acggctaatt ttttctctaa tataatgatt gcactctttg ctatttcctg tattttgcca
      781 tttatatttg ttatcgccat ctcttttacc gatgagtcca tattgtcgac tcacgggtac
      841 agtttttggc cagaaaagtt cagtatattt ggttatacgt acttgttcga ccagatgcag
      901 gataaaattt tccaagcgct tttcgtcact gtacttgtta ctgttttagg tacactgatc
      961 aatagcacgg ctacttcttt atatgcttat gcgatttcac ggtcaaactt tccattccgt
     1021 cgttttttta ccgttttttg tttgattacg atgttgttct caccagggat ggtggccaat
     1081 tatcttgtta tgacaaatct tcttcagtta aaagatacga tttgggcatt gatcttgcca
     1141 atggctgtca gtcccttcaa tattattgtg atgcgcacat tctttaaacg ttcggtctct
     1201 gattcgatta ttgaatcggc acggatcgat ggagctagcg aactgcgaat atttgttcag
     1261 atcgttttac cgttagctgt tccaggtatt gcgacaatca gtttatttgc cgcattgggt
     1321 tactggaatg actggttcaa tgctttacta tacatccaag atgataagct agttccacta
     1381 cagtatcttt tgatgaaaat ccaaagtaat attcaatatc tgacacaaaa ttcaggagca
     1441 ggaagccaat tgacaggcgg attagcctca gttccaggag aatctgccag aatggcgatc
     1501 gtagtcatct cgacgttgcc tattgcgatc agctatccat ttttccagaa gcattttgtc
     1561 aaaggtctaa caatcggcgg agtaaaagaa taaagaaaca aaaatataaa tcataaaatt
     1621 aggaggaaga aaaatgaagt tatggaagaa actagccttt acaggtgttt cagcgttgtt
     1681 gttgggaaca ttagcagcat gcggaggttc tggttctaaa gaccaggcag aagctagcaa
     1741 ttcggacact ttgcagatgt atcaaatcgg agataaacca gataatttcg atcaattgat
     1801 ggaagtagca aacaaacgaa tcgaaaaaga gatcggtgta aaagtcaata tcaattacat
     1861 tggctggggc gattacgaaa agaaaatgaa cgtcatcatt tcttccgggg aaaattatga
     1921 tatcgccttt gcgaacaact atgtttccaa tgcccaaaaa ggagcctttg ctgatttgac
     1981 tgaattagcg cctaagtacg ctgaaaaagc ttataatgat ttagatgaag catatattaa
     2041 aggaaatctg gtcaatggaa aattatatgc tttcccagtc aatggaaatg tctttgctca
     2101 acaagtactt acttttaaca aagctttact agataaatac gatctttcaa ttgatggaat
     2161 ccaatcttac gctgatgcag aaaaagtctt gaaggaattc catgaaaaag aaccaaacac
     2221 ggcagctttt gccattggac aaggcttcaa agtccaaggg aactttgatt atcctttagg
     2281 gaatacgtta ccatttgcta ttgatctgga tggagatgat tcgaaaatca tcaaccaata
     2341 cgataatgaa acgtatatcg gactgttacg tacaatgcat agctggtaca aacaaggact
     2401 cattccaagt gacgctgcaa cgagcaataa ggattatcca ctagaaggaa atacttggct
     2461 aatgcgtgga gaaacacaag gaccgtatga ttatggcgat acaattttga caaatgcagc
     2521 aaaacaagaa cttgtctcaa aagcaattac tgttccacta aaatctacag ctcaagcaca
     2581 aatggcaaat tttgtcgtat caaatacttc aaaaaataaa gaaaaatcag tagaacttct
     2641 agggctgctt aacagtgatc cagaattatt gaatgggctt gtttggggga tcgagggaga
     2701 agcatgggaa aaagtcgatg gttcagatga taaaatcaaa ttgctagatg gttatcagcc
     2761 aaatacacat atgtctgctt ggaatacagg aaacaacaag attctatata cacaagagtc
     2821 tattacggat gacatgattg ccaaacgtga tcaatcgatt gctgatgcag aaacttcacc
     2881 gattttagga ttcagcttca acactgacag tgtcaaaaca gaattaagta atatttctaa
     2941 tgtcatgaat caatatttag atggattgaa tacagggact gttgatccag atgaaacatt
     3001 accgaaattg aaagacgctt tggacaaggc tggctatgat aaggtattga aagaaatgca
     3061 aaaacaatat gatgagttcc gacaagaata ataatgaata aagacaaagg ggttgtgaca
     3121 aaagacgtgt cacaacctct tgttccgaat aaacggtgtt caaaagccag tttctcggta
     3181 ttaagctaaa tatatgtaaa aatgtggaaa gccatattca caaaattttt cttactactc
     3241 gaaactaagc ggctatttca caaactcttg gtaaacggtg ttcaatcagc taaccctcgg
     3301 tattcgctca caaaacgaca aaatatgagg aactattttt ccgtttgttt ctctaataat
     3361 caggtcaagg tgctgattgc ccaacctttt tttgtataat gagaaagtat aaacttaatg
     3421 tttggaggag aagatatgtt agggaaggtt ttggaagagt tatttatccg gatctgggtc
     3481 attataaaac taaccttgta tttctggata tatacatttg ccggcgggat catctttgga
     3541 ttaggtgcgg catggaaaac agtcaatgaa ctattttatt tgtacggatt cgagtataaa
     3601 gaaatcacaa taaaaagagg ctggaatata tataaacctg aataattcat agctttaatt
     3661 ctttggcaca ttttattgaa atatgtatca ctaatatccc tatcaactga ttttgactaa
     3721 aaagttactt caaacattca ctgttcaaaa atattgagga ataaattttg ggtgtaaggc
     3781 attttgagca tacttctccc tggataactt cccagtaaaa aaatcgttag attgttggat
     3841 tagatccatt gactggctcg cctcaggcga gcaaagaccc tgtagaattc attctgatac
     3901 acatgataac tagataattt gagatagact cgtctacccg tttggactaa tttcccagca
     3961 actttcagaa acttcaatcg aatagaatga atggtcattc ccttttggac ttcttcaaag
     4021 ccaatcgttc ttaagaagtt gactaagttg taagctatca agctgagcat cattcggaca
     4081 tgattctcca aaaagcgagg actgtctgtt ttgtcaaagt aaaagccagc tttcgcttct
     4141 ttaatgaagt tctccattgt gccacgtttg gcatagatag aaaagatggt atcaggagaa
     4201 acattttctg atagattcgt cacgataaat tcatgtcgaa agagtagttc gcccgcttca
     4261 cgtgttgagc gtatacacac gcgacgactt tgtgaccagg attgtgcttg ataagtggtc
     4321 gagaagtact gaacttctcg ttcttcccac ttttgattat cgccataaag tactgatttc
     4381 tcagctattt gacctagtct acgattattc ttcagtcgaa taacataatg actctttttt
     4441 gattcacacg aatcatacac accaggtgta gcgaacccgc tgtctccacg aaccaagatg
     4501 tcagtgtttg gtagagagtg attatagtgc tctaataaag gtgtgagaaa ctcctttacg
     4561 ccttttgatg tatattgatt tcctgaacgt agttcagctt ttaagaagtc accagtcaat
     4621 ccgtcaaaag ctaccaatgg atggtaacca taggtttggt agtgtgtatt ataattcgtt
     4681 tgttcttggt gaccaaatgt atctgaatgg gtcgaatcta aatcaatgat taattccgta
     4741 tcattacgga tgaggcgtgc tttatcgata agttcttggt tcaaagcttg aagttcatgg
     4801 atattctcct cagatagtcg atctaaaaat cgggaaagtg aagattgaga agcgagttct
     4861 ttcctatcta aaacagcttt aaacacagga tcttgtctca ggagattagc tgcagaatca
     4921 gctgagtaac cagcaattaa ttgcataatg aactgctcaa gtatggataa gttgtcatga
     4981 gtaaaatacg ctcgttcgtc ttcaatgtga atgtgttgct tagccagttc agaaaaacca
     5041 aagatgttca tcagctcttt cactaggacg agacccgaat cactcgataa ttgaccacct
     5101 gtatgagaaa tagtgatatt tgaattgaat tttacttggt ttttgtgtaa gctagtcatg
     5161 aaaagaactc ctttcttttg gttggtttag tcactttaac catagcagaa aggggttctt
     5221 ttttcatcac ttaacgggtg aagatgaaaa agtaattgta agctgccgtg aggcagtttc
     5281 acatggtttt aattaaaagt atgaattatt caggaatatc cataagaaat aaaaactgat
     5341 acactggagt aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct
     5401 ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca
     5461 atattggaaa aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca
     5521 accggcttgc cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga
     5581 gccatgattt cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca
     5641 cagccccatg aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg
     5701 ttagctacga atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta
     5761 gattttcgaa gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc
     5821 gtatggaaaa ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga
     5881 taatatgcga tataatacga acgtattgga taatataaac aataatatta gtttttcgta
     5941 tttgcagtaa atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac
     6001 agagtagaaa ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga
     6061 cgtaatgttc gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa
     6121 ggagatgcta aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt
     6181 attgattgca ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa
     6241 aatcagtgtc acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc
     6301 agggataggc gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt
     6361 ctttattgga tttacattga atgcatttat tgaaggagca atctacggat tctttttcta
     6421 tcgaaaagag atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat
     6481 caacttattt ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg
     6541 ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc
     6601 catttattac gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca
     6661 cgctaaataa aaa
//
LOCUS       DAZUMV010000130         6517 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000130 DAZUMV010000000
VERSION     DAZUMV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.133"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..826)
                     /locus_tag="ACG8FK_002405"
                     /pseudo
     CDS             complement(53..826)
                     /locus_tag="ACG8FK_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            862..2049
                     /locus_tag="ACG8FK_002406"
     CDS             862..2049
                     /locus_tag="ACG8FK_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HFS5097964.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDYQLPEG
                     SRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDV
                     NNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINE
                     YSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKN
                     YFN"
     gene            2782..3750
                     /locus_tag="ACG8FK_002407"
     CDS             2782..3750
                     /locus_tag="ACG8FK_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5097965.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3761..4576
                     /locus_tag="ACG8FK_002408"
     CDS             3761..4576
                     /locus_tag="ACG8FK_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS5097966.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4644..5354
                     /locus_tag="ACG8FK_002409"
     CDS             4644..5354
                     /locus_tag="ACG8FK_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5097967.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            5390..>6517
                     /locus_tag="ACG8FK_002410"
     CDS             5390..>6517
                     /locus_tag="ACG8FK_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS5097968.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2196 a         1002 c         1329 g         1990 t
ORIGIN      
        1 gccaataaaa cttagtccga tatgcggcgc gttctgtgcg ccgcatattt ttttagtttt
       61 caaagatcag accttgttgt aaatagatcc gtgcccgaaa gttataaaag ttgcgataac
      121 caaacgcaat tcgtttaatc agcttgatct tgttatacag cccctcagtg acgccgtttg
      181 agtgggacac cttaaaggca tttgaaatac ccgattggta gcgatggaag atcgtgaatt
      241 tctttttaaa cgactgaggt aacgtagatg aaattgtttt aagggtttct tgaaagccta
      301 ccgtatctcg tttaacgaaa taatagcgta ataattgaca aaaatgatac gcagttcgca
      361 attcttcgtt gtagcttaac agctcattaa cgatttctgt ttgactaatt ggtcgtttaa
      421 acagtcgatg ataatgccgt ttgagtgcct cccatgtcgt ggaatctttg agtaagagtt
      481 tccaatatcg cttgagccga cggtacttct tctgattttc agagtgtgag gttcgaaaac
      541 gattcatgat ctggacacgt aattgattga agctgcgatt gatgtgttgg acaatatgaa
      601 agcggtcagt tacgatctcc gcatttggaa agaccgtttt aagcaactga caatagctgg
      661 cattcatgtc catcactagg tacttgacct ttaaacgggc ctttctagga aagcgcagga
      721 agtattgagt tagtttgtaa agtctgcgat cttcaagggt cgtaaatagc tgattggttg
      781 tcccatcaac acaaataaaa ctcatggaac cagaagtggc aacattgtgt acttttcttt
      841 gcaatcttgt gtacaaaact attgcaaaac acagcgcata agaatcctta caaaacagta
      901 gatgaagcaa cccaattaga tcttgttata gttgttaatc agctcttaac gggttacgat
      961 tcaaaatggg tcaacaccct atacttagat aagtcacttg aatatcagaa cataattcag
     1021 gctttttcac gcacaaatcg tcttaatggt cctgaaaaac cgttcggaac cattcgttat
     1081 tatcgtaaag cacatacgat ggaacagaac attgaagagg ctattgagtt atactcaggc
     1141 aaacaagcaa aagggttatt tgttgaaaaa ttatataata acttagaaaa gttgaacgat
     1201 atattcgaag aaattaagtc cgtatttaat atttctggca tagaaaactt cagtcattta
     1261 ccggaggttc caggggaaat taagcaattt gtaaatcttt ataatcaact aatccattgt
     1321 tatgaatcag cgatgataca gggattcaca tgggcaaaat cgacttatga aatccaggat
     1381 tatgaaccta taacagtagt aatcacagaa tatgaattta atatcttggc acaacgttac
     1441 agcgagatag agagagaacc tagagacgga aatgatagta tagcgtatga cattgattac
     1501 caattacctg aaggtagtcg tagtcgtatt aattatgagt atttaaacac aaacttcaaa
     1561 aaatacatga atataaaaga agaacgtgat tcgaatttaa ttcaatcatt gttaaacgac
     1621 ttacatcgca catttgcgca attaccagtg gagttacagc catatgctga gatgattcta
     1681 aatgatgtaa ataatgggga attagttatc aaagaaggat gggaatttac tgattacctc
     1741 aatgaatatc agcttagtga agaggacgat tttatccaag atttagtgga ttcaaccaat
     1801 attaatgagg cattattacg tgagatgttg gatttacgct taaccgaggt aaacattaat
     1861 gaatatagtc gatttgataa attaaaatca agtgttaacg taggtcattt cagtggatat
     1921 atcgagaaaa atttaggtaa aatggtaata ccaatcaagg taaatatgct aattgacaga
     1981 ttattgcgtg catttttatt agaagatgta ttcgatgtta cagagtacat aaagaattac
     2041 tttaactaga gtacacctac atcttatagt acataaaaaa attcatcttt tttagtttaa
     2101 taattcacca aaaaggtgag taatgccatt tttgaaaatt gagcttgttt tatgtctaca
     2161 tctgtatccg tatataatcc aaaggattat aaactcagaa gagtctttat tttttctgaa
     2221 tataactact gtatcaaaaa caatcgatta tgagacaaaa caaaatgacc tcttttattt
     2281 aagaaaggag gtcatttttt gtatcataaa ttgtattaaa atcaatgtat cactttttaa
     2341 tcaattttaa gaccataaat tgcgtactaa aagaaattct gcaattcaaa ataaagcatt
     2401 ttaagccatt ttcaaattta agtgcaccca tctacccgtc aaatcacttt attttccact
     2461 ccagtagttt cctaattcaa ctgtgtgcaa aacgatttct tctaaacaaa tcaagacaaa
     2521 cttataaacc ttcatcccag caccataatt atttaaatca taggcatttt tcgaaattag
     2581 tatatgtact caagtttcac tgcagattat ttatattttt tagagaaata aatttttcct
     2641 attgacaaac acaaagaaag cggatacaat acaattatgc cacatgacgt tatatgacgt
     2701 tatgtaatat ctttacattt aatcagttac ttttattttg gataaaatta acatttttaa
     2761 aattttaagg aggaaatgtt gatggatgta gcaaaaacgg ttaacgcaat tgtagaaaaa
     2821 aatgatggag agatttctgg aatttatttg gtggcttgtg gcggttcgct ggtagatatg
     2881 tatgtctcag attattttct taaagccgaa gcatcgaagt gtattacagg cttatataca
     2941 gcgaacgaat ttaaccatgt aaccccaaag caattagatg ataaatctgt tgtcattcta
     3001 tgttctcatg gaggaaatac agcagaaacg gtaaaagcag gagaagtcgc tcaagctaga
     3061 ggagctgtga ctgttggtct gactcataat aaaactgcgg atttattaaa ggtttctgat
     3121 catagtttcc tttacgaatg gggagatgat gcaaaaatag tcaataatcc tatggccatc
     3181 attcttgatt taactgtatc tttggtcaat gttaccgaaa catttgaaca ttacggtaaa
     3241 ttcagagaag gcatgaaggt tatcgatacc gtaattgaaa atggcattaa gcaggtgcag
     3301 gatcgtatta aagtgtttgc tgataagtac aaagatgaaa caatgtttta tatcttaggt
     3361 agtggaccat catatggaca tgcttatggt ttttcaattt gctctttgat ggaaatgcaa
     3421 tggcttgatt caacttctgt tcattctgga gaattcttcc atggaccttt tgaagtaaca
     3481 gacaaagata cgaattttat tctcttgatg agcattggac gcactcgtgc tttagatgaa
     3541 cgggcaaaac gattccttga taaatatgca gaaagagtag aaatcgttga tgcaaaggaa
     3601 ctaggattgg atatgatatc agatgaagtt tctgaattct tcaatcccat tttattctac
     3661 agtattcttt cagaatatcg ttcagcttta gcagatgcac gcaatcatga tttagatgtt
     3721 cgtcgctata tggggaaagt ggagtactag gaggattaaa atgaaggtat taggcttagg
     3781 tgataacgtc gtcgataaat atgaaaatct gggaattatg tatcctggag gaaattcatt
     3841 gaattttgca gtttttgcaa aaaaactggg acatgaaagt agctttatgg gagtttttgg
     3901 ctcagatgag gaggcagctc atgttttatc agcaataaaa aaaataggac tggacaacag
     3961 tcactctcgc tttgaaaaag gcgagaatgg ctgtgccaga gtgaagattg atgagggcga
     4021 ccggattttc cttggcagca attcgggagg tgttactcgt gaatacccga ttcagcttac
     4081 tgaagaagac agagaatatt taaaccagtt tgaattgatt catatgggat tatatagcca
     4141 tgtcaatcat ctattagaag agttacaaaa tgtttctgga aagatttctt atgatttttc
     4201 tgacgacttt acggaagaag aggttcagcg ggcaattgat aaagttgatt ttggcttctt
     4261 ttctatagaa gacttttccg atgaagaagt aaagcagttt ttgatgaagc atttttgtga
     4321 gcgaaatgat gtattgattg caacccgagg agaaaaaacg gctattggtt atgatggagc
     4381 aaagttttat gaagtgaacc ccgtgctaga agaaccagtt gatacaatgg cagcgggaga
     4441 ttctttctta acagctttcc tactttattt tctcgaaggg gacgatatag tcaccgcgat
     4501 gaaaaagggg aatgaatttg ctgggaagtc ttgtctggtt gacggttcgt tcggctttgg
     4561 tattcattac gattgagctt gttttaaatt cctgcatttg ataacctaaa gaaagaaatc
     4621 ccggtaaagt ggaggttaaa aaaatgctgg atacaaatgc acaattacca ttgtacttac
     4681 aattaaaaga aatattaaaa aataaaatca atacagggga gtatcccgag aatacgaaaa
     4741 ttcccgctga atcagagctg atagaaagtt atgaggtcag ccgaatcaca gtgagaaaag
     4801 cagtagaaga actggtagaa gaagggtacc tacttaaaaa acaagggaaa ggaacttttg
     4861 tcaaccggca taaagttttt cgtaaaattg aatatgttat gggtttcagt gagagttgta
     4921 aatctaatgg ttttacaccg actagcattg ttttggaacg caagatcatt ccagcaactg
     4981 aagggatggc aaaaaaactc gatatcgctc aaggtgatga tgttttatat atccagagaa
     5041 agcgaatggc ggataacatc cccattatgt tagaaaacaa ttatttttct tatgaaagat
     5101 atagtttctt aatggaggaa gagctgactg attctttgta tgaagtactt aaagtccaag
     5161 gtaccttagc catcaatcct ggggaaactg cacttgaaat tgtcacagct gatgaccaaa
     5221 ttgccaaaat aatggaagta gctgttggga cgcccttttt ctttatggat actttaatca
     5281 acgatcaaaa caacatacca attcatgtag gtaatcaata ttacctggga gaagtatatc
     5341 gtttttactt atgatttcct gttttgattt caaggagtag attttattta tgaagcaagt
     5401 attgcggtca aaaatgattt ttgatggaga gcagccacct ttcaaaggtt atattgtaat
     5461 caatgaaggg atcattgaaa aggtggaaaa caactggaat tacagtgatc tattaaatga
     5521 aaacacggaa ttaattgctt atgatgatca attcgttatg ccagcaattc atgataatca
     5581 tgtatttttc agtggttatt tggctatgaa tgctggcata gatttaagcg atacatcttc
     5641 agctaacgag gctgtagagc gtattaaagc tgtaatgaac cataaaaaag cagatgaacc
     5701 aatttatgct catggctggg atgctgccct gtggaaaact gacccatcag aagagctatt
     5761 gaatgaaata aaccactttg gtccaatcac agcgatcgac aaaaatcgca gccattgttg
     5821 gatgaatgct attgctaagc agacttatgg ttttacagaa aaagaaacca gtgctgaaag
     5881 tcgagtatta ttaattaagg aaatgctagc aaacaaaaag cttttagcag aggtttacgc
     5941 tgaatttgaa gcattactgc tttcaaaagg tgttatatca atgaaagaaa tcgtttttga
     6001 tgacgcagaa tttctttcta atatacctca gaggaaaata cttagtaacc tatatgtgca
     6061 ggcagtccaa gaacctctac agcatgaact tctatatgag tataaggaaa aagttttccc
     6121 tgaaaaggtc cgctttggtg gagtgaagat catggtagat ggtgttgttg cggatagaag
     6181 tggagatatt tacggtgttt atcagtcagg agtagcttca cccgagattg actatgaggc
     6241 aattaaagca gaagttgctg cattcaatga agtagagatt ccttgctgtt tgacaacgga
     6301 aggagataaa gcgggttata aagctgcgtc aattcttgtc gaatttggaa agcggttacc
     6361 tgaaggcgtt tttaattcga ttagtgattt ggaaatggtt actcaaaaga cagcagaaaa
     6421 aatgaacgaa ggacaagtag tagcagaggt atatccacaa attctcggct tgaatcaaac
     6481 gttagaagag gcttacatgt caactgttat aaaaaat
//
LOCUS       DAZUMV010000131         6482 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000131 DAZUMV010000000
VERSION     DAZUMV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.134"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..549
                     /locus_tag="ACG8FK_002411"
     CDS             211..549
                     /locus_tag="ACG8FK_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097969.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            635..880
                     /locus_tag="ACG8FK_002412"
     CDS             635..880
                     /locus_tag="ACG8FK_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097970.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            900..3056
                     /locus_tag="ACG8FK_002413"
     CDS             900..3056
                     /locus_tag="ACG8FK_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097971.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDAPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            3068..3301
                     /locus_tag="ACG8FK_002414"
     CDS             3068..3301
                     /locus_tag="ACG8FK_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5097972.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3291..4157
                     /locus_tag="ACG8FK_002415"
     CDS             3291..4157
                     /locus_tag="ACG8FK_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097973.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            4172..6142
                     /locus_tag="ACG8FK_002416"
     CDS             4172..6142
                     /locus_tag="ACG8FK_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5097974.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
BASE COUNT         2497 a          888 c         1259 g         1838 t
ORIGIN      
        1 cttgataaac cagcattttt agccaataaa aaaagtgaaa taatttaatg gaaaatctct
       61 tttaatcatc gaaaatttat gttattatga taacataaag atgtaaatat cgagacgtct
      121 ttttcttagt tagatcattg acaagtgaac aaataagatt tgtcgatgat ataattaact
      181 tattaaaaat attaaaaagg aggtttttat atgttcgatt ctattaaaaa tttggttagc
      241 ggaatgacgc ctgatatgaa aactttagta caagttgttt tgtatttctt ggcattcgtt
      301 ttttcgttga aaccaggatt cactgcaatg aaattttttg gtgatgcaaa atggggagat
      361 ggattaaaat atgttggggc gattattgca atctttatta ttcctgtaat tacaaccgtt
      421 atgctttaca caatgggtac taaaacaggt aatgatttga accaaaaagc taatatcata
      481 tcagcactta ttccagttgt tggtatctat ctagtttcaa aatttccaaa aaatgaactg
      541 ttaggttaaa agtgaattga acaaatttgg ataatatgcc gatttgttca atttactttt
      601 taccaaaaaa ctacgctcca agcgtagttt agaagtgagg gaagaaagta aatgtaagat
      661 ggtttttgat agagaaaaaa tgttagctca cgccaacgaa gtattaatga gcagtctgaa
      721 aggtaccgaa ctggcaaaga taatgaatat gaatgttaac cagttctatg attatcgaaa
      781 tgggagtaaa aagattgaaa aagccagact tgaaacgctt attaaattcg aaaaagcata
      841 tgtatatatg ctagataaac agaaaagaac tattgactga aaaaaggagt tttacaataa
      901 tggcaaaatt caaagatatt accgaatcta ttaattatgt ggcagaaaca gttcctgtag
      961 ttgcactctc tttgaagcca caattactcg aagtgtacaa aaaagaagaa aaagaagcga
     1021 aggcaaaaaa agctaaagca aaggtatgga aagaattcga tgcgttgctg accaaagatt
     1081 taccaaagaa aaattatatg gtaattgaag atgtacaaaa gctcaacagt gagggagtta
     1141 tagatgagga tgatgctatt ctcgattctg agaagtatgt cgttctattg aagtcaagtt
     1201 tgaattatgt agctgatcta ccagaaaaat ttaaagagaa gctattagca ggtgtccgtt
     1261 ttatggatag tagtggtcaa ttattagtta ttccggatgc tcctaatgtg tttgaatcag
     1321 aagttgaaga agctacagac ttctatagcg cagtagagca attagaagta gctactaatg
     1381 gattagaacg aattgctgag accttcaact cttctgaaga agttgaattt gaagccgtag
     1441 cagaaaatgt accagatgaa aaagaagctc tttttgtgga tgataacaaa ccaaccgaag
     1501 cagaaaacca agaagtaagc atagaagaaa cacaaataca aacagaaacc taccaacaac
     1561 caaaaattga tgaaaaaccc gaatcccaaa gtgacaaaga aaattatgtt tatgaagctg
     1621 aaaaaatcac atctgaaagt gatgtaccga aaaacttaga tgctgatttt ttagaaaaaa
     1681 attttgaaca gatcattgct gacaaatttc aacctgtagt tttagatgag attaatgacc
     1741 ctgtcgattt aaacgaggag atgctatcca tacctgagtt gaaaccattg ctgtttttag
     1801 ctaaagagag tatgaacgct cgcatctcta ttgctaataa taaaatcaac agtgaattaa
     1861 ataagacgaa ggagcaattg atcctcaaag gaaagcaaat gttagctgat gagcttttgc
     1921 ggatagagga agcaacctca ttaaatggtc aaaatcagtt tacaaataca ctatcggttg
     1981 ctaaagagag ctacgaagca gaagcatcta aaattgatga tttagtgatg gaaaaatcca
     2041 atatcattcg taaagattat gatatggcga aacaggctta tttggatgct gaactaaaga
     2101 aactagagca acggtatgat gctgagcatt taccagaagt taaacaacaa gaagatgaat
     2161 atagaaaagc aactgaagaa agtcttgatc aacagtacca agaagcaatt gaatctatta
     2221 aaaacgatgc attgaaacaa agccaagaag caaaagaagt tgtggtggaa cgaattatca
     2281 attcattgaa accagaaatt gaaaaatcat taacagacgt gcaagaaaaa actttagaga
     2341 ttgtcgaaaa aacaaagtca gaaaatgaag ctgagttcca tgatacaaaa gagaaaacaa
     2401 cagatgtggt tgtcaaatat tttgcgaaca aagaagaagt tgaaaagcag gtagctaaca
     2461 acacgaaaga attagaaaca aaacttgtag agcaacaaaa aactatcgaa gaatataaag
     2521 atcgaatgaa agaggtcaaa gatttagcag agggttataa attaaatagc gaaaagttgg
     2581 cagaacaaag tgaagcttta agagaagact tatctcgttc ccgaagtgat ctagctcaaa
     2641 tggcagaaaa aacgaaatca ttagcagttg ctacagaaaa tcaatcaagt tcaaaacgtg
     2701 attttacaag taaaaagatg gctactatca tttctgtcgc tgcaatggct tgcggaacga
     2761 tcttgggatc gtcctacttt attagtcata ataatcaaca agcacaggct caaactcaaa
     2821 catctatttc gagagtggaa aattcgattg ggacaaatac gaactctgct tcaacaaata
     2881 gtagctatga agcaacacca actacagagt tcaaagttgg gaaagatgtt gtcatcagta
     2941 ttgatgggaa aacaactaag gctgaagtga tttctattga tggagaaaaa gcagttgtga
     3001 aaactccaga cggaaatcag tacaaaattc aagctcactt atctgataat aactaaggag
     3061 ggctattatg cgagaagaaa aaatagttta tccagtaatt caaaacaatc aagaaaacat
     3121 gacctcttct cagcacttac aggaagcaca gctcgtgtat caggtaaatg aactaccaca
     3181 aacgaacgag acaatgagca agtcaatatc tttaattggc ttgcttattg ttgcaatagt
     3241 gataactgta ggtgtttacc gtaagttgaa cgtgaaagga agtatttctg atggcagata
     3301 actcaaacta tggatttaaa cataacttac atcaaaaacc ttctgaaaca attgtcagag
     3361 ttgaatggct tgaaaagcat gtcaatagca cctattctgt tacagcaaaa agattaattg
     3421 tatcagctat agttgttgca gtcgttgttc ttggatcaat tgtttactta gtttctgata
     3481 cgattaaact gaaatttatt tttgttccaa cgttattaat gataattgga tttcttattc
     3541 taattagcca gtatatgttt attgatccat ctagtggttc attgatttct ggttctatta
     3601 gactgttcaa atcagtagtt aattcagcaa gagtatctat ggggaaaaag aaaccatttc
     3661 gaggcacaga gattttaaaa gtgaaacaag atggaacaat cgtatatcga gatgggagtg
     3721 tagcattaat gttcaatcta gatggggcta catcactaac cgcttttcca gaagaagtta
     3781 tggctcagga atcgaaatca atacaatatc aaaataccag agatcgttcc ttagctgaaa
     3841 tcaaaatcac ttcatctcaa cctcagaata cggaaagaca aactcgaaat atgaaaatac
     3901 aaaaacgaaa tgcaaggagt aacaaagcag tctttgacat aatagatcag caagaacgat
     3961 ttgtatcaga atatattgat ggtgtgaaaa caacagtaat ccaatatctt atcttgaaat
     4021 ctcaatctaa gatggtattg gatgaaggtg tggaacgttt agaaaactat acgagtagag
     4081 gactttatta tagtgtcact cagttgtcag ctaaacaaac aaaagaaata ttatctgata
     4141 tttatgcact gaaatagagg tgagagatta aatggaaatt atgggtagat cacaactatt
     4201 aaatttcaaa aaaaagagaa aaactaaaaa agaacaaaaa actagaaatt caaaggtagt
     4261 gaataatacc tttgttagtg atttaacatc tttagttggt tatcaattta caccatctta
     4321 cattaggaca ggtaagcggt ggggaacaat tgtgaaatta gtgaatactt atggaatgaa
     4381 tagaaattat ggatttggtt ggatggttaa agctattcct gaagtgagag tagaaggggt
     4441 aaaagcctat ctaattgaag cagataagcc agtgtcacaa aaagatcaac aagaaataat
     4501 gcacgagaat gttgggggat caacgaatag ttatgaaaat aatttagaag gaaaaaattc
     4561 agatgctgaa attagcaaaa ctgttatgcg gattatggat ttgaatacag catctttact
     4621 agacagcatg gcaaataaaa ttattgattg gaagctagaa atcctattag tatccgattc
     4681 tcctaataaa atcagcgaac agctaattaa actccaacgg ttgtatgatg aaaaaatgta
     4741 tggattaaag cttatgtcat atggaggaga tcaacgtgat ctgtttgata gacttttaag
     4801 tgaaccaatc gggaatgtct atgagtatac gtctatgagt tcggattttg caggaaatga
     4861 tcatgcaatt cgcaaaggtt tagatgatgc gaatggatgg gctatcggca ctatgtctac
     4921 tagctacgca agtggtgtag cttttatggc actggatgat tcttttacaa gtaaagtttt
     4981 agtggcatca cacaaaaacg ctacaattaa aggctatgat gaaaaaatct cagcatcatc
     5041 actttggggg caattgattg ctaataatgc aatggcaaat cataaacgaa cttttcatat
     5101 tgtcttaaat aaatttgatt actttggtga tccaactggt agaacatttg ttgccaaacc
     5161 ttcgttaaac agtgtaatag aaaaggttaa tttggctgaa gaaggtctta actctttgga
     5221 gctttttggt aatcaaaagg acattggtga tttatataga cggaatctcg ataaactaac
     5281 tcagattttt tatttgttag caggcagaac attagattct gcaactagag ttttattgaa
     5341 aaaagaatta ggtaactttt atataagtca gaaactttgg gatattaatt atgaagaacg
     5401 ttcaaaactt ttggtgaaca gtagtgatac aatgccagtt cttggggatt ttattagcta
     5461 tttgactaat gctgaaacaa aagccaaaaa atctggtact gaaaaagaag tcgatcgtat
     5521 tattgtatta aaagacacat tgagaacagc attagacagc ttctctagga tgtttaatca
     5581 aagaacgaca ctgaaagaaa tcggtaaaag tgacaaatta cagttttact ataacttagg
     5641 tgatctaaga gcagaaacca atgttatgga agctcaattt ttaaatacct ttgatttcat
     5701 cacatcacaa gcaaaaaaag atgatattgt tatgattcac ggcttcgata agttatctat
     5761 tgaaaccgtt gggctaatta aaaaaagatt aacaatgctt gaagaagatg gaattcgtct
     5821 tgcctttcta tttgatacaa tcggttcagg agaagacaat gaatacaaag aagatattga
     5881 aacagctgat atattcaata cagatggaaa aatttaccaa agttttgaaa gtgattttaa
     5941 ttacactatc attggaaaga tgaccagtgg agagttaatg aagtatgaaa aaaaagttgc
     6001 tcctgctaga aaaggagaag atgatcaaaa actttctgtt gatttgagac agtatttgac
     6061 aacaggaaga agtaataaat ttcagatcag gagaccagtg gatctgacta ataattttgt
     6121 tattggagag tttattcttt aagttttatc tttgattttg agtgtataag gaaaggataa
     6181 gaaaaaacat gtatatttga gactaaaaac agtcttagag aacatggtac gttttgtgaa
     6241 aagagaacta tagaaatttc ataaagtgaa accttactga gtgaaataat tatagtggag
     6301 gttaaataat gaaaccaaac ataaaaattt tagatcgaat atttctaggt agagacacag
     6361 aagtaatcct aattcaacat gaagaaggat ttgaagttag tataggtatt cagaaacttc
     6421 aaaaacctca ttattgtaat cagttgtata aaaactttac tgatgaagaa aaagcgagag
     6481 tt
//
LOCUS       DAZUMV010000132         6464 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000132 DAZUMV010000000
VERSION     DAZUMV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.135"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..1057)
                     /locus_tag="ACG8FK_002417"
     CDS             complement(8..1057)
                     /locus_tag="ACG8FK_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5097975.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(1139..1828)
                     /locus_tag="ACG8FK_002418"
     CDS             complement(1139..1828)
                     /locus_tag="ACG8FK_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5097976.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1934..2302)
                     /gene="rplL"
                     /locus_tag="ACG8FK_002419"
     CDS             complement(1934..2302)
                     /gene="rplL"
                     /locus_tag="ACG8FK_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS5097977.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2355..2858)
                     /gene="rplJ"
                     /locus_tag="ACG8FK_002420"
     CDS             complement(2355..2858)
                     /gene="rplJ"
                     /locus_tag="ACG8FK_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS5097978.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3062..3189)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3268..3957)
                     /gene="rplA"
                     /locus_tag="ACG8FK_002421"
     CDS             complement(3268..3957)
                     /gene="rplA"
                     /locus_tag="ACG8FK_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS5097979.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4061..4483)
                     /gene="rplK"
                     /locus_tag="ACG8FK_002422"
     CDS             complement(4061..4483)
                     /gene="rplK"
                     /locus_tag="ACG8FK_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS5097980.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4826..5494
                     /gene="sdaAB"
                     /locus_tag="ACG8FK_002423"
     CDS             4826..5494
                     /gene="sdaAB"
                     /locus_tag="ACG8FK_002423"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS5097981.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5507..6379
                     /gene="sdaAA"
                     /locus_tag="ACG8FK_002424"
     CDS             5507..6379
                     /gene="sdaAA"
                     /locus_tag="ACG8FK_002424"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS5097982.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
BASE COUNT         1906 a         1333 c         1226 g         1999 t
ORIGIN      
        1 gaaaaaatta actagcacca cgggtaaata cagtattatt tcggttgctt gctgcttcac
       61 ctagcccttg tactcgtgtt tgattggcga cttgaccaaa ttgattcccg acgtctaata
      121 aaataatagc taaaagcaaa atggccaaat ggcttccgcc aaccaataaa gcaataaagc
      181 ttattgtttg cataacaagt cccatataca cgatttttct ttcagaataa gaatctgcca
      241 gtcgtcccac gataggtgca gcaactgctc cagacaaacc aaaaatcgct aatatccctg
      301 cttcgaatgt tccccaattg taggcgggac tgctgatata aaaaatcaac gtagaccaaa
      361 agatcgaaaa tgttccaaac atgaaaaatc cactgactgc cgcttctctt aatagacgtt
      421 gagaagcaac taaatgcggc aaacttttaa gtgaatcaag gtatgaaaca gctgtactac
      481 tttgctggac agaacgcggc attttccagt aaagcagaag tgtaagaatt ccgactgcac
      541 ataaagcaaa taaatagatc atccgccaag aaaaaagact ggcaacaata ccagataccg
      601 tccgagaaag caaaatcccc acgagtaaac cgctaagcaa tgtacccatg accttccctc
      661 ttgcttttgg tcccgctagt accgcaccgt aaggaatgat gatttgcggc acgatcgaaa
      721 gtaatcctat taaaaaagaa gcgcatgcaa ataacgtaaa cgaaggcgca aaaaaagcgg
      781 ccaacagaga gacggcagat aacgctacca ttcgaatgat caattttgga cgatccacca
      841 cgtcccctaa aggaactaaa aacagtaacc ctaacgcata gcctaattgg gtcaacatcg
      901 tcaatatccc gactgcactt gtgctaacag ataaactagc tgctactttt gttccaatcg
      961 gctggatgta atacatattg gcaacaacaa cgccacaagt gattgataat agtaaaataa
     1021 gcgaagttgt taaaggtgtt ttttcattac tcttcattga tttcccccat ttcctatctt
     1081 agtttacaac ttatggaaaa gattggaaat agaaaaatac aactattcgc cacacttatt
     1141 atttgtgctt ttgttttcct tgttttttct tgcgatcttt ataaatgaag acaactaacc
     1201 ctactagcac agtaacactt gcaaccaacg tgactgcagt tttgttatat ccgtgaaaca
     1261 gagcttctcc accacacgca tacaacacag acgaagggat gactccaatc acgatcgaca
     1321 atatgatttg ctgtagaggc aatttcatcg catctgcagc aaaattgacc atagaagatg
     1381 gaataaccgg tatcatatag cctaacatta cacctatttt tggatgtttc atttgtcgga
     1441 ttgcttgcac ccaatgattc gtttcggttt tcttatctaa aaattttaag cgatgcataa
     1501 ggaaaattgc agtgatgtta ccaagagcat tacccaaaat gttgatgatc gcacctgtca
     1561 gtggtccaaa gctcaaacca gcaatgactc caactactga agtaggaata cctggaacag
     1621 cacacatcac cgctgtcaat acgatcaaga gtatcgatgc aaaagtccca tgcgaacgaa
     1681 tatgtgcgag tagctctgcc ttactcgctc tcggatttaa aaataattgg atgtctgtct
     1741 gatattctaa atacaaccga tagcccacta ccagtatcag aatgattccc aacgagatca
     1801 acatcaatcg gattattttc tttttcatat ttcctcctga aaaaaatcta tattcattaa
     1861 ataatagttt aacataaaaa caaaaaagca ccgaaatcca acataggatc tcagtgcttt
     1921 taaatagtaa aaattattta acagttacag atgcgccaac ttcttcaagt ttagctttca
     1981 attcttcagc ttcttcttta gatacgcctt ctttaactgg tgcaggagcg ccatcaacta
     2041 cagctttagc ttctttcaag cctaaaccag ttgcttcacg aactgctttg ataactttaa
     2101 ctttttggtc tccagctgca gttaattcta cagtgaattc tgtttgttct tcagcagcag
     2161 cagcgccacc agcagcagct acagctacag gagcagcagc agatacgcca aattcttctt
     2221 caatagcttt aactaggtcg ttaagttcta ggatagttgc ttcttttaac tcagcaacga
     2281 tgttttcaat gttcaatgcc attataattt cctccgttta attgttcttt tttatttaat
     2341 gctttatgcg gcaattaagc tgcatctcca tcttctttgt ctgccactgc tttgacagcg
     2401 taagccacgt tgcggactgg cgcttgtagt acagatagca acatagatag aagtccttcg
     2461 cgatttggca attttgccaa tgctgtgatt tcttccaatg aagagacttt accttcgatg
     2521 ataccgcctt tgatttctaa tgcttttgct tctttagcaa attcgtccat gattttcgct
     2581 ggtgcgacta catcttcgtt actgaatgca acagctgtag gtccagtgaa tacttcgtcc
     2641 atgccttcaa ggccagcttt tttagctgca cgtgaaagaa tagaattttt gataactttc
     2701 atttctacgt tagcatcacg aagttgttta cgtaaacgtg ttacttcatc aactgttaat
     2761 ccgcggtagt caacgactac tactgatgca gctgctgtaa atttttcgct tacttcatca
     2821 acgattgctg ctttttttgc gataattgct tcactcattt ttgagtttca cctcctgaat
     2881 tttgatggaa gagttttttg cactgaaaag gcaagtcact tttatagtta ctttgctcaa
     2941 ccagtattat tcatcaccta ttcacttcgt tcatagtgat tctactcgct ttgctcgaaa
     3001 ggaaactcct ttcgctttgc tcaaccagta ttgttcatca tctcttcact tcgttcatag
     3061 tgattcacaa taaaaaactc tatgccaccg cagacataga gggactattg aatatattca
     3121 ataaacgtcc tcggtaggaa attaaggctt gatgccacct actgtcttcg gtacaagtta
     3181 attattaacc tcaactagct tagcatgcta aactagttga gtcaagaatt atttttcagt
     3241 tggatcaatc aacattctaa tatttaatta gaaagaagct tggtcaacat ggataccagg
     3301 tccaaatgtt gttgtgatcg ttacattttt catgtattga cctttagctg ctgatggttt
     3361 agcttttagc aatacatcat ggattgctgc aaagttttca actaattttt catcgtcaaa
     3421 tgaaactttt ccgattggta catggatatt tccagctttg tccacacggt aagttacttt
     3481 accagctttc acttcgttta ctgcttttgt tacgtccatt gtaactgtac cagttttagg
     3541 gtttggcatt aagccttttg gtcctaatac tcgtcctaga cgacctacag tagccatcat
     3601 atcaggtgtt gcaacaacta catcaaagtc aaaccatccg ttttggattt tttgaaccat
     3661 gtcgtcgtca cctacgaaat ctgcaccagc tgcttcagct tcttttgctt tgtctccttt
     3721 agcaaaaact aaaacagttt gtgttttacc agttccgttt ggtaatacaa cagcaccacg
     3781 gatttgttgg tctgcttttt tagggtctac gttcaaacgg tatgcaactt caactgttgc
     3841 atcaaatttt gcaatgtttg tttcttttgc taaagctact gcttcggcaa caggataaac
     3901 tttacttgaa tcaacttttt ttaatgcatc ttgcattttt ttgctctttt tagccatttt
     3961 ggttcctcct tgattgtggt tataacggtt gtacctccca cgtgttctat atctttcaat
     4021 atagagccaa ctgagaagct gctgtcttag ggtgtataga ttattctaca gtgatcccca
     4081 tgcttcgtgc agttccttca accatgcgca ttgctgcttc aacgttagct gcgtttaggt
     4141 cttccatttt taattcagcg atttctctta cttgatcgct agaaactttc gcaactttat
     4201 ttttgtttgg ttcgcctgaa cctttttcga ttttagctgc ttttttcaat agaacagcag
     4261 ctggcggtgt ttttgtaacg aatgtgaatg aacggtcttc gtatacagaa ataacaaccg
     4321 gaatgatcaa acctgcttga tcagctgtac gagcgttgaa ttcttttgtg aatcccatga
     4381 tattgatacc cgcttgacct agtgcaggac ctactggtgg agctggagtt gctttacctg
     4441 caggaatttg caatttaacg attttttcta ctttctttgc cacgagacat acctccttaa
     4501 gtccgtgatg tggttaattg gagatgcata tttctcctcc cacgttttat ctgatctgaa
     4561 aaaatccaga caaataatgt gcaaaacaat gcacactggt acagtatagc aaatttattt
     4621 gttttttcaa gatttttttc tgctattttt acattttgct tgaataaacg ctaaatttat
     4681 tctaaaaaaa caacaatttt tcgttctcat ttcttctttt atgttacact tgcgctagca
     4741 tggttcaaca gatgaaagca aacaataaca cggtttttta gacccttgta caaagattcc
     4801 tacataacaa aatgaggtgg agaaaatgaa agatttaagg tatagaagtg tctttgatat
     4861 tattggacct gtgatgattg gtccaagtag ctctcacact gccggagctg ctagaatcgg
     4921 gaaaatcata cgtagtatat ttggcgaaca gcctgattct gtagatattt atctatatga
     4981 atcatttgca aaaacctatc gggggcacgg aacagatatt gcattagttg gcggcctgtt
     5041 agggatggaa cccgatgatg aacgtttagc tgattctttg aaattggcat atgaagccgg
     5101 tatggaagtc ttgtttgttc ctaagagtga aaaagccgaa catccaaact ctgtaaagat
     5161 gcttctaaaa aaaggaacac gaaagctttc agtcactggc atttcgatcg gcggtggcaa
     5221 tatccagatt tctgaattga atggattcaa aatttcttta agcatgggta ctcctacctt
     5281 gatcgttgtc caccaagatg ttccaggtat gattgctaaa gtgactagta tactttctga
     5341 gaccaatacg aatatcggca ctatgacagt gacacgggaa tcaaaaggtg aaaatgccat
     5401 tatgataatt gaagtagatg atcctcaagt agaagaaacg gtaaaaagac tcaaacagct
     5461 gccaaatatc gacagcgtaa attatttcga ttaaggagct aaaaaaatgt ttttatcgat
     5521 tgaagaatta gtagatcaag caaaagctta tccaagtgta gcagaattga tgattgcagt
     5581 tgaaatggaa atgagtggcc gttcccgtag tcacatcatc gagatcatgg aaaaaaacct
     5641 ctcagtaatg gaacaatcaa tcaacgaggg aaaagaaggt gttacctccg tcacaggaat
     5701 taccggtggt gatgcaaaaa aattagatga ttatttaaat gaggcagact ttttaagtgg
     5761 agaaacaatc ttaaaagctg tacgcaatgc tgtagctgtt aatgaagtca atgcaaaaat
     5821 ggggttgatt tgtgctacac cgactgccgg aagcgcgggt gttgttcctg gtgtgctgat
     5881 ggctgtccga gatcggcttg atttgacaca tgaacagcaa ttggaattct tatttaccgc
     5941 tggcgctttc gggttagtca tcgcaaacaa cgcttctatc agtggtgcag aagggggatg
     6001 tcaagcagaa gttggttctg caagtgcaat ggcagcagca gctttggttt gtgcaaaaaa
     6061 tgggactcct catcaagcag cacaagcagt agccattaca ttgaaaaata tgatgggact
     6121 gatttgcgat cccgttgcag gcttagttga agtcccgtgt gtcaaacgta atgctttagg
     6181 ctcttctcaa gcttttatct ctgctgatat ggcattagcg ggtatcgaaa gtgtcattcc
     6241 gccggatgaa gtcgttgctg caatgtacca agtcggacgt caaatgccgt cgattttcaa
     6301 agaaacagca gaaggtggct tagctgttac gccaactgct aaacgacttg cggaagagat
     6361 tttaaataag agggcgtgat aaagctcctt aatttcgagt attaagagat tagttatctt
     6421 cataacaaaa aacgaagaga ggactaattg cccttctctt cgtt
//
LOCUS       DAZUMV010000133         6371 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000133 DAZUMV010000000
VERSION     DAZUMV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.136"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(226..903)
                     /gene="nth"
                     /locus_tag="ACG8FK_002425"
     CDS             complement(226..903)
                     /gene="nth"
                     /locus_tag="ACG8FK_002425"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS5097983.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(910..1608)
                     /locus_tag="ACG8FK_002426"
     CDS             complement(910..1608)
                     /locus_tag="ACG8FK_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5097984.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1876..3060
                     /locus_tag="ACG8FK_002427"
     CDS             1876..3060
                     /locus_tag="ACG8FK_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS5097985.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3066..3509)
                     /locus_tag="ACG8FK_002428"
     CDS             complement(3066..3509)
                     /locus_tag="ACG8FK_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5097986.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3735..4022)
                     /locus_tag="ACG8FK_002429"
     CDS             complement(3735..4022)
                     /locus_tag="ACG8FK_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5097987.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4193..5545)
                     /locus_tag="ACG8FK_002430"
     CDS             complement(4193..5545)
                     /locus_tag="ACG8FK_002430"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS5097988.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(5817..6263)
                     /locus_tag="ACG8FK_002431"
     CDS             complement(5817..6263)
                     /locus_tag="ACG8FK_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5097989.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1724 a         1268 c         1059 g         2320 t
ORIGIN      
        1 ctggacttat tttataggta tattggaagg cattattttc tacatcgggt attttcccaa
       61 taaaaataac aacttttatt tttatcgcaa taaattgtaa taagttacca tcctagctac
      121 tgagtatgta catttacttt ttcatgcaaa aaagataaac gaaacagctt ttctccataa
      181 taacaagata attcatacat ttatttgcaa ttaaagaaac cacgtctaca ggcgtggttt
      241 cttttttagc gccttttctt ttagacgcat ccgatttttc ccatcttgac atattgatag
      301 caaaggacat acctcgcact tgggatttct tgccgtgcaa tgatatctac caaaaaaaat
      361 cagtgtatga tgtgtcttta cccataattc ttgtggtact ttccgcatca gtgtttcttc
      421 tacttccatg accgtagcat ctagtttgca gatcctcagg cgttttgaga cacgttccac
      481 gtgggtatct actgcgattg ctggtatccc gaatgcatcc cccaatacga catttgctgt
      541 ctttctgcct acaccaggaa gagacatcaa ttcttctctt gacgttggca cttgcccatc
      601 gaaacgttca atcagctgct gtgcacacgc tttgatgttt tttgctttat ttcgatacaa
      661 accaatcgtt tttattttaa gaatgatttc atcgattgaa gcttctgcta acgcatcggg
      721 tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc actgatacat ctgtcgcctg
      781 ggcacttaag atcactgcaa tcaataactc aaatggatta ttgtgcttga gttctccatg
      841 tgcttcggga aacattccat acatcgtttc tagagcttcc attgtttttt gtttgcttaa
      901 catattccgc tactcctcca accaattttt cagtgagact ttcggcaatt cttcttgttg
      961 agctgactgt tctacttctc tttgaagaag ctgctgtttt cttcgttttt ggtcttcttc
     1021 cacttgttgt tttgtacgta gattttttct ttcccacgat agcaaaatgc ggtcaacgta
     1081 attaaaacta taagcctgat ttaatacggc ttcccgcagt gctagcttca aaatttccgg
     1141 ctgataatgg tcttcttcta gccattgtcc gatccgctgg aactcgatcg aagaaagcgg
     1201 gcgtccaaat tcttgttcaa ataattgata cactgactgg atttcttttt cttgtgtttt
     1261 ttcttcgtat tttttttctt gtgtttttaa atactcacct aatagatcat agatggggta
     1321 aagattatat cgatctgctt ttttaccgtt gacaatcgtc gtttctatct tgataaatcc
     1381 atttgttacg agacgatcca gtatttgaaa aatctgatct tgtttgattc ccatatccat
     1441 agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc tgtgccatat gcaactgtag
     1501 aacaaataaa aattcttcag attgtagacc gatcctatga taatttttaa gtaaaagatt
     1561 agatatgact gtttgtccag cgcttaagta attttctaaa ttcaacatga tttctcctcc
     1621 gctcaatcac agtatacgta aaaaaaacat gggaagcaag aacaggtttc ttttctattt
     1681 acttcactga tcggtcagat ttacaatgtc gtctacttga tttttgtttg ttcatgtttt
     1741 tgatgcgtat cttcactttt cggaataaat aaattaagta gatttcgctg ttccttagaa
     1801 tgttgacaag gaaacaaaat tactatacac tatttttgtt tccaagttaa cattattgca
     1861 taaaggagtt tttacatgta cagtttcaca aataaacatt ctaaagtttt tggatacgtc
     1921 tctgtcttat tcactggatt aggatttact atcgtcagtc ctgtacttcc gtttttaacg
     1981 ctgccttatt ctcactccat acatcagcaa gctttctata tcactttgct catgtctgtt
     2041 tatgctctag ctgccttctt atctgccccc attctcggta gcttaagtga tcattttggg
     2101 cgtcatccga tccttattat cagtttatta ggatcaagta tcggatattt gctttttggt
     2161 ttaggaaatt cgatatggat gttgttcctt ggtcgtatca ttgaaggatt gacggcagga
     2221 gaaatctcca ccttatatgc ttactttgcc gacatcacgg aacctaatga aagaaccaaa
     2281 gtttttggat ggatgggtgc tttagtcggt ataggaacaa cgctgggtcc tattataggg
     2341 ggtctattag ctgagttggg gaattctgtt cctatattta ttggagcact ctttactttc
     2401 ttaaatgccg tttatggcta cacatttatg ctagaaagcc ttccaataaa aaaacgttca
     2461 gtagatttaa gtttctccca tgtaagacct tttcaccaat tacaacaatt attcaagatt
     2521 tcatccgtta ttcctttatt gacagcaggt tttgctgttt ggttggcagc tggctctctt
     2581 caatctatct tttctcaatt ttcgattgac gcctttcagt ggaaggctgg gttggttggt
     2641 ttaagcttct ctttgattgg aatcttggat actgtctcgc aattgttcat tatgcctcga
     2701 ttgctcaaaa aattttcaga acagcagatc acaagaatag ggatgttcag tgaaatcctc
     2761 gcctatctat tcatcacatt atctggaatt cttctactgc cgattttgtt tttatttggg
     2821 attatctgtt atggtttcgg tgattctatt tttactcctg tattcaatgg ccaactatcc
     2881 aattctgtct cagataacca gcaaggtctt gtaatgggcg gtacccaaag tatacaatct
     2941 ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt atgctgcagc accatttctt
     3001 ccgacggtat tcggattttt gcttatgatt tttgcctatt atcactacaa taaaaaatag
     3061 tagcttcatt tgctacttcg tataaattct ctgtatcgac cgataaaacg aaatactgtg
     3121 tcaaattctt cttttgacat attttcaaat acagtagcat ctcgctgtac ccatttttga
     3181 tggagttgct cgtgtctatt ccttattttt tgtccgattg gcgtcaattg aaaatagatt
     3241 tcttttttat tctcttcgct ttgataagtg tttatgatcc ctctagctat cagttttttt
     3301 gttagcttac tgatggctcc tctagtcatg taactagcat tcgctaattt tgtcacgtta
     3361 ggatcaggga ttttcccgat atgttcaatc acttctagtt catttagagt aaatcctgca
     3421 aattcttttt ccatttctgg tttattacga tgaataatct cattagctaa atccagatat
     3481 tcttctatta gcagttgtct tttatccatt tctacacctt ctttttcttt cattatattg
     3541 tttttttgtt tcctattcaa caaagataaa aatattttgt aggaaaaccc aatctgaaac
     3601 caagaaaaaa aggggttgac aaagcttgtc ataacccctt tttctgatta aaagatagta
     3661 aagagacagt catcggtaat tgtgatgatt ttttgtcatc acttaaggat gagcggcctt
     3721 ttcaaaaact gtctttaaag atcatcatca gtttctagca catatggctg accttcatat
     3781 gtgtactctg tattcaatgt gccgtcattt tcatctgagt agatgcgtct actgaaagga
     3841 aaacgatgag aaacattatc tttggcgtca tttgctggca tcgtgtaata ttcctcttgc
     3901 gtttcattca actgagagag tacttgatct gccaactcat caatattgat cgttaatttc
     3961 ttcgaagcac ttttataatc ttctaaaaat gcatcttgtt ggacaaaata ttctttattc
     4021 atcttctttc tcacctttct tttcaaaatc aaataaagct gcttgttctt ttatgtaaca
     4081 gctttatctt tgctgcttat tgtgacaaaa aattcgaaga aagacaacta attgtacttt
     4141 tcacaaaaaa agacagaggg tcgtgacaaa attgtgtcac aacccttttt ctttattgtg
     4201 cagtttcgat ttcgcctagc gcttcttgtg caagtaggtt caagtaattc catgggcggt
     4261 caaaatgtgg ttggaagaag aaatctgaaa gtgcgagatc ttctacagtc attttatttt
     4321 gaacagctaa agacatggta tttgctgatt gtgtaatgtc gtatttggac atgaactgag
     4381 cacctacgat acgattcgtt cctttttcat agactagctc catcattact ttttcagtag
     4441 ttggcatgaa ctctggacga taattatctt caaatacagt cgcttggact tctagatcgt
     4501 tcaaagttgc gctttctttg gtcacacctg tcgaaccgat cgtccagcca aataggtaaa
     4561 gtcctgaagt tccttgagta ccacgatacg ccattttatg atctgtcaag ttatttccca
     4621 caagtaaacc ttgacgaacg gcatttgttg ccaaaggaat atagttttgc gtgccacttg
     4681 gattgtagtt gactactgca gagtctccag ctgcaaaaat gtctggatca cttgtctgca
     4741 tgtattcatt cactttgatt gccccgtttg gcagtgtatc tacttttcct tcgatcagtt
     4801 ttgtatttgg acggaagccc acacataaaa tgaccatatc tgtatcaaat gtatctgttg
     4861 gtgtagctac ttgtttcact tttccttctt catctgtgat aaattcagta acattttcgc
     4921 ctagcgcaag agtaacaccg cggtctgtca attctttttc taagatatct gtgaatggtt
     4981 tatctagata tttattcaag atgcgatcta atccatcaat caatgtcact tgttttccag
     5041 attcgacaaa agcttctact aattcaatac cgatatatcc accaccaaca attactacac
     5101 gttttgcttc tttcgcttgt gcaataattt catttgcttg attgtagttt ttgcatagga
     5161 gtacattttt cgcatcgatt ccactaatcg gtgggataat aggccatgaa ccagttgtca
     5221 tcactaattt atcataagaa accgtttctg tttctcctgt tttcagatct tttgccgtta
     5281 ctgttttatt ttctgtatcg acgtctgtta catcgtgttc cattttaact gttgctccta
     5341 atgaagataa ttcttcagga tttgagtaga acaagcctgc tgggtccttt actactccgc
     5401 cgacataaag tgcgataccg caagataaga aagaaatatt gtcatttctt tcatatactg
     5461 tcacttctgc ttctggatga ttttttaaaa tactttttac agctgcagta cctgcatgag
     5521 tacagccaat tactactact ttcatttatg gtccctccga ttaacatttt gtccttcgac
     5581 aaaactacta taacagaaat aaaacggata caacagttta atgcttgtga attcacaaag
     5641 gtttgtttgt agtttcattc cttttcaagc ataagaaccc aatatctaca agattcttat
     5701 tgtgataata ttcactttga tatctaagat tgtgaataaa agacaaaaaa ataaaaaagc
     5761 tagcgttttt ttacacagct agcttttttt aggtataaca cttatagaaa ctgtttttac
     5821 atttgcgcct tttttgctga ctctttacaa tccggacaga gtccatacaa ttccaaccga
     5881 tgttcattaa tctcaaaatc agtcagtctg ctcgcagcca tttctatatc ttcaagccct
     5941 gggtaatgaa aatcaacaat cttaccgcag ttttcacaaa ttacatgata atgacgcttt
     6001 gaactgaaat caaatcggct agaagcatcg ccgtacttca tttcttgaac aaatccaatc
     6061 gaagtaaata agcgaaggtt attgtaaaca gtcgcaacac tcatatttgg aaaccgatgt
     6121 tcaagtgctt tataaatctc atcagccgtt ggatgggaat gatgttcaat caaatattct
     6181 aaaaccgcgt accgttgagg tgtgattcga atgttctctt tttttaactt ttcgataacc
     6241 tcttcgacta acttattttc catgtaaaca ccccacgttg ttattctcgt cttatcatac
     6301 tatttttaca ttttaaaatc cacttaacat gaaaagaaaa ttcaatttta ttgtatttta
     6361 atgaaaaaag a
//
LOCUS       DAZUMV010000134         6344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000134 DAZUMV010000000
VERSION     DAZUMV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.137"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..483
                     /locus_tag="ACG8FK_002432"
     CDS             106..483
                     /locus_tag="ACG8FK_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5097990.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            471..809
                     /locus_tag="ACG8FK_002433"
     CDS             471..809
                     /locus_tag="ACG8FK_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5097991.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            943..1386
                     /locus_tag="ACG8FK_002434"
     CDS             943..1386
                     /locus_tag="ACG8FK_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFS5097992.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1648..2463
                     /locus_tag="ACG8FK_002435"
     CDS             1648..2463
                     /locus_tag="ACG8FK_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5097993.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2473..3135
                     /locus_tag="ACG8FK_002436"
     CDS             2473..3135
                     /locus_tag="ACG8FK_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5097994.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3340..5004
                     /gene="alsS"
                     /locus_tag="ACG8FK_002437"
     CDS             3340..5004
                     /gene="alsS"
                     /locus_tag="ACG8FK_002437"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS5097995.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            5017..5727
                     /gene="budA"
                     /locus_tag="ACG8FK_002438"
     CDS             5017..5727
                     /gene="budA"
                     /locus_tag="ACG8FK_002438"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS5097996.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            5857..>6344
                     /locus_tag="ACG8FK_002439"
     CDS             5857..>6344
                     /locus_tag="ACG8FK_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS5097997.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SF"
BASE COUNT         2071 a          997 c         1353 g         1923 t
ORIGIN      
        1 cttagtttta atagaaatga gtattgactt ggagtacact taaagttgta gaataaataa
       61 aatacataag taaagtggca agaaattaag agaaagagtg atcagatgaa tatcaaacaa
      121 gtcagtgaag aaaagggcat ttcggccgat acattgcgct attatgaacg aatcgggttg
      181 attccaccag ttaatcggac aaaaggcgga atccgtgatt atacagaaga agacctaaaa
      241 tgggtcgact ttacgatctg tatgcgaaat gccggattat cgatcgagtc tttgattgaa
      301 tatattcgcc tatctagcga aggagaagca actgtatttg agcggcgtaa gcttttgatt
      361 gaagaaagtg aacttttgac gaaaaaaatt gctgaaatgc aagaatgttt ggaacgttta
      421 caaggaaaaa tcaagaaata tgatcgtgtg ttatctagaa ataaaatgga atgtatcaag
      481 taaaaaaatc aaggagcatc gctatggagt tacgcttact aaaatatttt tggacaattg
      541 cagaagaagg aacaatctct caagccgcag aagttctaca tcttacacaa ccaaccttaa
      601 gccgacaact gaaggagtta gaagaggaac taggaacaga actgtttgta cgcgaaaacc
      661 gcaaaatgat cttgacagaa gcaggttatt ttctaaaaaa gccgagcaga agaaatttta
      721 gatttaacga atcaaaccac tcaagaattc gagaacagaa aaaaacaatt attcagcggc
      781 cgatttacga tcggctgtgt ggaagctgat aattcgaata cattggcact attactagaa
      841 aagttgatcc gtgattatcc cagcgttcaa tttcatatcc acagcggaac aagtgatgat
      901 atcacggagt gactagaaaa aagcctgtta gatttagcga ttttgctaga tccagtagat
      961 acagagaaat acgaaacgat tttgttacca agaaaggaaa aatggggatt gctcatttta
     1021 gaaaattctg ttttagcaaa aaaagacgtg ataacaaaag aagactttgc gaatgtacct
     1081 cttttgatct caaagcgtcc agaaatacag cgaatgtttg ctgactggtt aggcataaaa
     1141 aaggagcagt tgctgatcac aggaacgtac aatttgattt tcaatgtatt tgcgctaatt
     1201 gagaatcagg taggtgctgc tttagtcata gaaggtgctg taagtcatct ttcaaagcga
     1261 gtgacattcg ttccgcttga cccaatgatt gaaacgaact gcatgctagt ctggaaaaag
     1321 aatcggattt tatcacctgt agtcaatgaa ttgattcacc gtttcaaaga agcttttgaa
     1381 cagtaataag tatctctaat cgaagaaatt tcaatacaaa agcgagacgt atgacagtct
     1441 tgcttttgta ttttttcatt tttaaatcaa aaaaaataaa gagatttaca taacttgtta
     1501 aataagcgct tacaaacata agaagaacgg aaatgaatca gctataataa ccgttgttct
     1561 ggattgtaat cattacaaag aaatgaagat tataactttg gaggtgttcg aaatgaaaga
     1621 aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc aatggttggg tatgattctg
     1681 ctgattgtat tagctttatt gacgatcatt ccttttttat ggatggtttc tgcttcattc
     1741 aaagcaaata atgaagtgtt cacgattccg attcaatgga tccctaaaac ttggcattta
     1801 gagaattatc tggttatttg gtctcgtatt cctttagtga cattttttaa gaatacagct
     1861 tttttaagcg ttgtgatcac gttgattcag ttgtttactt ctagttttgc tgcttacggg
     1921 ttttctaaac taaattttaa agggcgcgat attttatttt tagcatatat cggcacgatt
     1981 gctgtccctt ggcagtctta tatgatcccg cagttcatta tgatgagaaa agtgggttta
     2041 acatatacgt tgtggtcgtt ggtgctatta caagcgttca atgcttttgg tgtgttcttg
     2101 ttgaagcaat actacagtag tatcccagat tctttatgtg agagtgcgcg gatcgatggc
     2161 ttatcagagt ggggaatcta tcgcaagatc attttacctc ttacaaaacc ggcgattgcc
     2221 agtttgacga tcatcacatt cgttaataca tggaacgatt atatggggcc attcatttat
     2281 ttgtcttcta cggaaaataa aacaatccag ttagggttga aaatgtttat tgggctattt
     2341 gatgcagagt acgcattaat catggctgcc tcagttgttt ctgttcttcc tgttgctttt
     2401 atatttttag cgatgcaaaa atattttgta gaagggatcg cttcatcagg aatgaaggaa
     2461 taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg tttatatgga attcttttat
     2521 tggttgtatc aatcattggc aagtagcctt tgtttttggt gtgtcaatat cttgtatata
     2581 gcagctgcgt tccttttgtc gatagatgga aggaatgtct tttcctttat tttttcttta
     2641 ctttttattg gtccaggttg catcgtgatg ataggtatga cagaagaatt agcaaaagaa
     2701 aaaagttttt caccagtacg tttgttcata aagacgtttc gccaattttg gctaaaggga
     2761 tttttttatt ggctatttgc ttggatagtt agtgtgataa tgatttttga ttgtttcttt
     2821 tttatacgtt tttcttatgg aaaatggttg attccgttgt ttgttttact tgcttgttta
     2881 tcagtttcct ttagcatcaa ctactggtat tttcaggtga gaaatccagc gtcgaaacca
     2941 aatcaagttc tacggatagc gttctactat acgttgaaaa aatggtatgt gagtttacta
     3001 gattttttac tattgacaag tttgtttctc ttctttttcg taaagcccca atggtgtatt
     3061 ctactaggtc cttctatcgt atttggtttg atttacttca acaatcgtaa attgatgaga
     3121 actatggatc tgtagacttt ataaattgaa actttcaatt aagaatgaca gtttttatgt
     3181 aaaaactgtc attctttttc attttttgaa acatatggct aaacaaatga aataaaacgt
     3241 gctatacttt tgaatgaaac aggtttagaa actgtatcaa ttatttgtga ttatttctaa
     3301 ctaataatta aaaataaagc ataaaaagga cgatgttcaa tggacgaaaa aatgaaacaa
     3361 ggttcgaaat ctgtagtaga aagtttgatt aatcatcatg ttgattatgt attcggtata
     3421 ccaggtgcaa aaatcgatgg tgtatttaat gaattagagg atcaaggtcc tgaattaatc
     3481 gtgactcgtc acgaacaaaa tgctgcattt atggcacaag caatcggacg tattacagga
     3541 gagccaggag tagttatcgc aacaagtggt cctggtgcta gtaacttagc tacaggtttg
     3601 gtaacagcga cagcagaagg tgacccagtt ttagcaattg ccggacaagt aaaacgaagc
     3661 gatctattga agttgaccca tcaaagcatg gataatgcag cactgttcca gccaatcaca
     3721 aaatacagtg cagaaatcca agatccagaa acaatttcag aagtgattgc caatgcttat
     3781 cgtatggcta agagttcaaa aaaaggcgcg agctttatca gtatcccgca agatgtagtg
     3841 gatgcacctg tccaagggaa tgtcatcaag ccattgagcg atcctaaatt aggttctgct
     3901 tctgcagatg atatccgtta cctagctgaa cgcattagag aagcaaaact accggtacta
     3961 ttagtaggta tgcgaggatc aagtgaaaaa gaaacgttag ccatcaggca attagttgga
     4021 aagacagcat tgcctgttgt agagacattc caagcagctg gagtgatctc acgtgaatta
     4081 gaagcgcatt tctttggtcg tgtgggttta tttagaaacc aaccaggaga tatgcttttg
     4141 aaacgaagtg atctagttat tgctattggc tatgatccaa tcgaatatga agcaagaaac
     4201 tggaatgcgg aaaaagatgc gcggatcatt gtgatcgatg aagcgccagc tgaaatcgat
     4261 ccttttatgc agcctgaaag agaattgatc ggtgatatct cagccactct tgatttattg
     4321 acaggttcat tagagcctca acaagtgtca gaagatgcaa aagaatattt agcatctctt
     4381 caagcagaat taacggagcg ggacattgtt caatcaaaag gagaagcagg tatcttgcat
     4441 cctttagaag ttattaacac gcttcagtca aaagtaacag atgatatgac tgttaccgta
     4501 gatgtaggta gtcattatat ttggatggca cgccacttta gaagttatga acctcgtcat
     4561 ttgttattca gtaatggtat gcaaacgctt ggtgttgcat tgccatgggc gatttctgct
     4621 gcattggtgc gtccaaatac acaaatcgta tcagtttccg gcgatggggg atttttattc
     4681 tctgcccagg atttagaaac ggcggtacgt aaaaaattga atatcgttca tttgatttgg
     4741 aatgatggac actacaatat ggtagagttc caagaaaaaa tgaaatatca gcgggcgtca
     4801 ggtgtagact ttggtccagt tgattttgtg aaatatgcag aagcttttgg cgctaaagga
     4861 atccgagcta caagtgtaga ggaattagaa aaagctttag aagaaggatt tgcgacagaa
     4921 ggacccgtaa ttattgatat tccgattgat taccgtgata atgagaaatt gggagaaaca
     4981 atcttaccag atcaattcta ttaaggaagt gtcgacatga ctgaaaaaat cttataccag
     5041 cacgggacat taggagcttt gatggctggc ttgatggatg gaacagaaac gattgctcat
     5101 atattagaaa aaggaactct gggattggga acattgcatg ggcttgatgg tgaagtcatc
     5161 tttttagatg gcgtagctta tcaaggaaga tcagatggct cagttgccca attagatgga
     5221 agtgaactta ccccctatgc agcaattaca gattttacgc cagatactag tttttctgtc
     5281 tcaaaaactg cagatggaga acaattgaaa aaagacatat tagtaagaga agccggggaa
     5341 aacctatttt tggcagtgaa gataactgga ttattcaaaa atatgcacat ccgtatcatg
     5401 ccgaagcaag aaaaacctta cagaagacta gcaaaaattt cagagtctca accagaattc
     5461 caacaaagca atgtccaagg aacattagtc gggttcttta cgcctgaatt attccaagga
     5521 gttgctgcag cgggatttca cctgcatttt atcgatgata cacatacatt tggcggacat
     5581 gtaatggatt ttgaagtggc tgaaggaaaa gttgagatta gccgcatctc gtcccttgta
     5641 cagcattttc cagttgatga tccgactttt gttcacgaaa cgattgatta tgctgattta
     5701 gcagcagaaa tccaacaagc agaatagaaa aagacagcga gatgcgttca aggtgattaa
     5761 cctgacgtat ctcgtttttt tgaggagaaa acctagcgtt tacaaaaaaa ctgagctata
     5821 atcttctcca tagatcaaaa aggagcggtt tttatcatgt ggacagaaaa acagaaaaaa
     5881 gatgcatacg aaattttatt attacagcaa caaggattat tagaagcaga agataactgg
     5941 ttagcaaatt tagcaaattc atctgcattg ttgaatgaga ctttgcctga gactgtcttt
     6001 gcaggatatt atttattcga tggtaatgaa ttgattttag gaccgttcca agggcgagtt
     6061 tcttgcacac gtatcaagat gggaaaagga gtttgtggag aatcagctga aaaacaagaa
     6121 acattgatcg ttgatgatgt caaaacccat gcaaattaca tttcctgcga ttcagccgca
     6181 caatcagaaa tcgtagtgcc aatggtaaaa gaaggtaatt taatcggtgt tttagatatc
     6241 gattgcggag taacgaaagg gtatgatgaa atcgatcaag catttctaga aaaatatgta
     6301 gatcttttat tagcaaattt taatataaat gctttttcat ttat
//
LOCUS       DAZUMV010000135         6317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000135 DAZUMV010000000
VERSION     DAZUMV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.138"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8FK_002440"
     CDS             complement(99..1229)
                     /locus_tag="ACG8FK_002440"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS5097998.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8FK_002441"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8FK_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5097999.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8FK_002442"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8FK_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5098000.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4549)
                     /locus_tag="ACG8FK_002443"
     CDS             complement(3485..4549)
                     /locus_tag="ACG8FK_002443"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5098001.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8FK_002444"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8FK_002444"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098002.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8FK_002445"
     CDS             4880..5692
                     /locus_tag="ACG8FK_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098003.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8FK_002446"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8FK_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098004.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1876 a         1299 c          996 g         2146 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctcta
//
LOCUS       DAZUMV010000136         6295 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000136 DAZUMV010000000
VERSION     DAZUMV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.139"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(125..313)
                     /gene="rpmB"
                     /locus_tag="ACG8FK_002447"
     CDS             complement(125..313)
                     /gene="rpmB"
                     /locus_tag="ACG8FK_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS5098005.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            583..945
                     /locus_tag="ACG8FK_002448"
     CDS             583..945
                     /locus_tag="ACG8FK_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5098006.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            997..2679
                     /locus_tag="ACG8FK_002449"
     CDS             997..2679
                     /locus_tag="ACG8FK_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS5098007.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2796..4832
                     /gene="recG"
                     /locus_tag="ACG8FK_002450"
     CDS             2796..4832
                     /gene="recG"
                     /locus_tag="ACG8FK_002450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS5098008.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4881..5882
                     /gene="plsX"
                     /locus_tag="ACG8FK_002451"
     CDS             4881..5882
                     /gene="plsX"
                     /locus_tag="ACG8FK_002451"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS5098009.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6004..6246
                     /gene="acpP"
                     /locus_tag="ACG8FK_002452"
     CDS             6004..6246
                     /gene="acpP"
                     /locus_tag="ACG8FK_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5098010.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2106 a         1094 c         1432 g         1663 t
ORIGIN      
        1 ttcataattt gttcgtataa caaaaataaa cgcaaaaaaa gggtaggaat caaaagtccc
       61 acccttaaaa atttttatta aataaaaaac cgttcactat ctgaacggtc caagtatcat
      121 tcaattaaac gcgttcaact tttcctgatt tcaaagcacg agttgacacc caaacttttt
      181 taggttttcc gtcgaccata acgcgaactt tttgtaagtt aggtttaaca gtacgttttg
      241 ttgagttcat agcgtgtgaa cggttgtttc cactttttgt cttgcgacct gtaaagtaac
      301 aaacttttgc catggtaatt tcctcctttg ccttgatttg gagcaataga ataattcagc
      361 tcataccaga ctaacatatc atatagaatg ggaatttgca agtacatttt catagataca
      421 attgacaatt gagaagaata attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg
      481 tttttgagta ctttgggtaa ctgcctttta tttcaagttg cgctatgata aaatatgata
      541 gaagaaaaat gggagttttt tacccgttaa ggaggctcat tcatggctgt aaaaattcaa
      601 acgtcagctg gaaccattga aattaccaat gatgtaattg caacagttgt tggtggcgcc
      661 gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa atcaaattaa agataatatt
      721 actgagattt tgcgtaaaga aaattactca agaggagtag ttgtccgcca agaagataat
      781 ggtattgctg tagatgttta tacgattatc agctatggta cgaaaatctc tgaagtttcc
      841 cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat tattaggcgt aactgccaat
      901 tcagttaatg tctttgtaca aggtgttcgt gtactgccag actaggcagg agactggctg
      961 gtaactcagc agtcgatctg aaggaggatt atctaggtga gtgtaacaga aataagtgca
     1021 agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac
     1081 gtcaattcat taaatgtatt ccctgtgcca gatggcgata cgggaacaaa tatgaatcta
     1141 tctatgacta gtggagctaa ggcagtagta gattcaacct cagaaaaagt aggagaatta
     1201 gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg gaaattccgg cgttattttg
     1261 tctcaattgt ttcgtggatt ttctaagcaa attccagatg ttgtctcatt aaatgcttct
     1321 gatcttgctg cagccttcac tcatggagtt gaaactgcgt ataaagcagt aatgaaacca
     1381 gtagaaggaa cgattttgac agtagctaga gaagcagcga aagctggaga aaagaaagca
     1441 aaaagtactg atgatgtcgt tgaagtaatg acagctgttg taaaaggagc aaagcgtgct
     1501 ttagctaaaa ctccagactt gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga
     1561 gggcaaggac tgctgtttgt ttatgagggc tttttaagtg cattgaatgg agaattccaa
     1621 gcggatgaaa catatgaacc ttctccagca gaaatggatg atatggtcaa tgcagaacat
     1681 catcgcagtg tccaaggtca aatggcgaca gaagatatcc attttggtta ctgtacagaa
     1741 atcatggtaa aaatcggaga aggacctaca gtcgatagta cattcgatta cgaagaattc
     1801 cgtaactatt tagatggtat aggagattct ttacttgtgg tcaatgatga cgagatcatc
     1861 aaagtccacg tgcatacaga aaacccagga gaagtcttga attatggaca aaaattcggt
     1921 tcattagtca aaatcaaagt cgacaacatg cgcttgcagc atgagacgat tttggaacac
     1981 gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta
     2041 gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt
     2101 atcagtggtg gacaaacaat gaatccaagt acggaagaca ttgttaaagc gattcaagaa
     2161 gtaaacgcag ataaagtgat tgtcttgcca aacaataaga acatcttcat ggcagctgat
     2221 caagcagcag aagtagcaga tatcccagta gcagttgttc cttcaagaac gatttcccaa
     2281 ggattgacag ctatgctttc attcaatgat caagcttctt tagaagaaaa caaagaaacg
     2341 atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc
     2401 tcaattgacg gtgtagagat caaagaagga gactttttgg gcatgatcga tggaaaaatc
     2461 ctgatctcta acccagatat cacagcaaca gcgttaagta cactagaaca aatgatcaat
     2521 gaggacacag agatcgtaac gatcttggca ggtgatgaag gaacagttga acaagcaaat
     2581 gaatttgctg agaaactaac agaaaaacat cctgatttgg aaatcgagat ccaccaagga
     2641 gatcagcctg tctatcctta tctattttct gctgaataat ttctgtgtca gaatatcttt
     2701 ctaacacagt attcattcat aaaaggcctt cggttagtga tcaggtatga tcgctgacca
     2761 agtccttttt ttgttaaagg agggaggaaa aatttatgcg ttctttagat gatccaatca
     2821 ctatgctggc aggagtcgga gaaaaaagag cagctagtct tgccagcttg ggcatccaaa
     2881 cgattgaaga cttactgact tattatcctt ttcgctacga agatattcag gaaagaaatc
     2941 taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg
     3001 tgatgaatcg atttggttat aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg
     3061 atgtgttcaa cgtttctttt tttaatcaac cttatctaaa agaaaaagtc attttatcgg
     3121 aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa agccttgaac gggatgaaga
     3181 ttttaggttc tcaaacaatg gatgactttt cgccgatata tcacgtcaac aaatcaattc
     3241 gtcaaacgac attagttgat ctaatcaaaa aagggtttca agaatacggc acattgattg
     3301 aagaaaatct gccaaacaac cttgtggaga aatatcgact gcttgaccga gcttctgctg
     3361 taagaagtat gcattttcca aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg
     3421 tttttgaaga gttcttttta tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag
     3481 cagaaacgaa tggtttggag atcctttatg atgtccaaaa attgaaacaa tttactcaaa
     3541 agctaccttt tgaactgaca aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc
     3601 ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag
     3661 tggttgccgc gattgcgtta tacgctaccg ttacagcagg tttccaaggt gcgttgatgg
     3721 tgcctacaga aatcttggca caacaacata tggaaagctt gcaacagctg tttgatccca
     3781 atgaagtaac catcgcattg attacggggt caacgaaagc aaaagaacga cgtgagctcc
     3841 ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg cacacatgca ttgatccaag
     3901 atggagtcga atttcagaat ttaggtctag ttattacgga tgagcagcac cgttttggcg
     3961 tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc agatgtctta tttatgacag
     4021 cgactccgat tcctagaaca ttagctatta cagcatatgg tgagatggat gtatctatca
     4081 ttgatgaact tccagctgga cgtatcccga tcgaaacacg ctgggtaaga acacctcaat
     4141 tggacagtgt actggattgg acgaaaaaag aactggcaag aggacatcaa atgtatgtca
     4201 tctgtccgct gattgaagaa tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg
     4261 aaaaacttcg tgaactattt gcaccaaaat atgaagttgg cttgttacat ggtaaaatga
     4321 agaatcagga aaaagaagta atcatggaag ccttcaaaga caaccagatg cagatattag
     4381 tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca
     4441 tggatgcgga tcgatttgga ttggcacagc tgcaccaact tcgtggacgt gttggacgcg
     4501 gaagtgatgc ttcctactgt atcttagttg ctaatcctaa aaatgaacta ggtgtggaac
     4561 ggatgaaaat catgacagag accaccaatg gttttgtact aagcgaaaaa gatctggaat
     4621 tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg
     4681 ctgacttggt gacggatgga aatgttttgg aggtagccag agaagaggct gcagcccttt
     4741 ggcaaataaa ggattggcag atgcttcctg agtatcagcc tttagcgcaa agcctccaaa
     4801 tagaaaatca ggataatcgt tatttcgatt agcagactgt gctatacttt atactgatga
     4861 cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc aatgggcgga gatcacgcac
     4921 ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga ttttccagaa gtagaattct
     4981 tactttacgg aaaagaagca gaaatcaaga aatacttaac aaatgaaaaa aacatcacga
     5041 ttatccatac agacgaaaaa atcaatagtg atgatgagcc agtaaaagca attcgacgaa
     5101 aaaagactgc atcaatggtc cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg
     5161 tattttcagc agggaataca ggcgcgctac tagcagcagg gttgttcatc gtaggacgta
     5221 tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg
     5281 ccggtttcga tatgctggat cttggcgcta acgcagaaaa caaaccagaa catttgttgc
     5341 aatacggtat tttaggttct ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg
     5401 tcgcactctt aaacaacgga acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct
     5461 atgaactcct tcagaatgaa acaagcttga attttattgg aaacgtagaa gctcgtgagc
     5521 tactcaatgg tgtggctgat gtagtggtca cagatggatt tacaggcaac gctgttttga
     5581 aatctattga aggaacagca atgaatatga tgaatctatt aaaatctgct attctaaatg
     5641 aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag
     5701 cagaaatgga ttattccaaa catggcggtg ccgttttgtt tggcttgaaa gcaccagtca
     5761 ttaaaactca tggagcaaca ggccccgatg ctgttcgcta tacgatccgc caaatccaca
     5821 caatgcttga gacagatgta gttggacaat tagtcgaaca atttgaaaaa agcgaagaat
     5881 agtatttgga tatctgaagc gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa
     5941 tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa
     6001 aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag
     6061 atagataccg ataaagtgac ggatgaattg aacatcaaag atgatttaaa agctgattca
     6121 attagtatta tggaattcgt tttagagctt gaagatgaat tcggtacgga aatttcagat
     6181 gaagacgctg aacaaattga aacagtcggt ggcgcagtag attatatcag cagccatctt
     6241 aaataatttc aaataaaagc aaagaaggat aaaaagctgc atgcagcttt ttatc
//
LOCUS       DAZUMV010000137         6173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000137 DAZUMV010000000
VERSION     DAZUMV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.140"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8FK_002453"
     CDS             725..811
                     /locus_tag="ACG8FK_002453"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5098011.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG8FK_002454"
     CDS             complement(932..1621)
                     /locus_tag="ACG8FK_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098012.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACG8FK_002455"
     CDS             complement(1628..1906)
                     /locus_tag="ACG8FK_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098013.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACG8FK_002456"
     CDS             complement(1866..2780)
                     /locus_tag="ACG8FK_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5098014.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQVFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG8FK_002457"
     CDS             complement(2762..3142)
                     /locus_tag="ACG8FK_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS5098015.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG8FK_002458"
     CDS             complement(3592..3879)
                     /locus_tag="ACG8FK_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5098016.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG8FK_002459"
     CDS             complement(3899..4123)
                     /locus_tag="ACG8FK_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5098017.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG8FK_002460"
     CDS             complement(4578..4748)
                     /locus_tag="ACG8FK_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098018.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG8FK_002461"
     CDS             complement(4763..5296)
                     /locus_tag="ACG8FK_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5098019.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG8FK_002462"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG8FK_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5098020.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1634 a         1152 c         1041 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atacttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZUMV010000138         6147 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000138 DAZUMV010000000
VERSION     DAZUMV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.141"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..2715
                     /gene="adhE"
                     /locus_tag="ACG8FK_002463"
                     /gene_synonym="adhC"
     CDS             118..2715
                     /gene="adhE"
                     /locus_tag="ACG8FK_002463"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS5098021.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2944..4050
                     /locus_tag="ACG8FK_002464"
     CDS             2944..4050
                     /locus_tag="ACG8FK_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5098022.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4067..5091
                     /locus_tag="ACG8FK_002465"
                     /pseudo
     CDS             4067..5091
                     /locus_tag="ACG8FK_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5097..5576
                     /locus_tag="ACG8FK_002466"
     CDS             5097..5576
                     /locus_tag="ACG8FK_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS5098023.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5592..>6147
                     /locus_tag="ACG8FK_002467"
     CDS             5592..>6147
                     /locus_tag="ACG8FK_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFS5098024.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT         1992 a         1080 c         1377 g         1698 t
ORIGIN      
        1 atttaaaaat aactgtgaaa aaaaatcaca aaaaagtgtt tacatagaaa aataaatatt
       61 gtatgatata catgtgaatt atttaacaaa taaccatgac ccattaggag gataatgatg
      121 gctaaagtaa tagaaaaaga aaaaacgaag acaattgacg tacaagcaat gattgatgat
      181 ttggcagaaa aagcaaatgc agcattgaaa gaaatggaaa gctttgatca agaaaaagta
      241 gaccatatcg tgcatgaaat ggcaatggca gctttggatc aacatatgcc attagcgaag
      301 atggctgtag aggaaacagg ccgcggtgtt tacgaagata aagcaatcaa aaatatgtat
      361 gcgtccgaat atatttggca taatatcaaa catgataaaa cagttggtgt gatcaatgaa
      421 gatgtccaaa aagggttgat tgaaattgct gaaccagtcg gtgtggtttg cggagtaact
      481 ccaacaacga atccgacttc cacaacgatt ttcaaatcaa tgatcgcgtt gaaaacaaga
      541 aatccaatcg tttttgcttt ccatccaagt gcccaaaaat cttctgcaga agcagcacga
      601 gtggtacgtg atgctgctat cgctgccgga gctcctgaaa attgtatcca atggatcgaa
      661 catccttcga tcgaagcgac ttctatgcta atgaaccatc caggtatcgc gatcgtctta
      721 gcgacaggtg gagctggaat ggtcaagtct gcttactcta caggaaaacc agctttaggt
      781 gtaggtccag gtaacgtacc agcttatatt gaaaaaacag caaaaattaa acgtgcagtg
      841 aacgatttga tcgtttcgaa aacatttgat aatggaatga tttgtgcttc tgaacaagct
      901 gtgatcgtgg ataaagaaat ctatgcagca gtaaaagcag aattccaagc acaccaagtt
      961 tatatcgtaa aaccagatga attgcaaaaa ttagaagatg cagtgatgaa cgaaggaaaa
     1021 tatgcggtca atccatcaat cgttggacat tctgcgatgg aaatcgcaaa attggcagga
     1081 atcagtgtac caaaaggaac gaaaatgctg attgctgaat tggaaggcgt aggcccagat
     1141 tatccgctat caagagaaaa actttctcca gttcttgcca tgatcaaagc taacaacacg
     1201 gaccatgcgt ttgatttgtg tgaaggtatg ttgaatcttg gaggattagg acatacagca
     1261 gttatccatt cagaaaacga agagttgcat gtcaaattcg gtcttcgaat gaaagcttgt
     1321 cgtattttag tgaatacacc atctgcagaa ggcggcatcg gtgatatcta taacgaaatg
     1381 attccgtcat tgactttggg atgcggttct tatggtaaga actctgtttc tcgtaacgtc
     1441 tctgctgtaa acttaatcaa tgtcaaaact gtagcgaaac ggagaaataa catgcaatgg
     1501 ttcaaattac cgccaaaaat cttctttgaa aagaactctc tattgtactt agagaaaatg
     1561 gaaaacgttg aacgtgtcat gatcgtttgt gaccctggta tggtccaatt cggttactgc
     1621 gatacagttc gtgaagtatt ggcacgacgt aaaaatgatg tgaagatcga agtcttctca
     1681 gatgttgaac cgaacccatc gacaaatacc gtatacgcag gaacgaaact gatggcagac
     1741 ttcaagccag atacagtaat cgctcttgga ggcggttctg caatggacgc agctaaggga
     1801 atgtggatgt tctatgaaca cccagacaca tctttctttg gtgcaaaaca aaaattcttg
     1861 gatatccgta aacggacata taagatcgac aaaccagaaa aaacacaatt cgtatgtatc
     1921 ccgacaacat cagggactgg ttctgaagta acgccatttg cggtcatcac tgacagtgaa
     1981 acacatgtga aatatccgtt agcagattat gcattgacgc cagatgtcgc aatcgtcgat
     2041 cctcaattcg tcatgtcagt tccagcatct gtcacagcag ataccgggat ggatgtgtta
     2101 acacatgcaa tcgaatccta tgtttcagtc atggcttcag attatacacg tggattgagc
     2161 ctacaagcaa tcaagttagt ctttgactat ttagaaaaat cagtaaaaac acctgatatg
     2221 gaatcacgtg aaaaaatgca caacgcttct acaatggctg gtatggcgtt tgctaacgca
     2281 ttcctaggta tttgtcactc agtcgcacat aaaatcggcg gggaatacgg gatccctcat
     2341 ggacggacaa atgcgattct tttaccgcat attatccgct ataatgcaaa agatccacaa
     2401 aaacatgcga tgttccctaa atatgactat ttccgtgcag atacagatta cgcagatatc
     2461 gcaaaattct taggtctgaa aggcgaaaca acagaggaat tagtcgaagc tttagcaacg
     2521 gctgtttatg aattaggaaa atctgtcggc atcaacatga gtctaaaagc tcaaggagtc
     2581 acacaagaaa cactagatac aaccgtagac cgtatggctg aattagctta cgaagaccaa
     2641 tgtacgacag ctaatccgaa agaaccattg atctctgaat tgaaacagat catcattgat
     2701 gcttacaacg gataagcaaa agaaagcgta gaaaaaagat aagaaaatag atgaaaaaga
     2761 acttggaaaa cgttgtttct ccaagttctt tttttaattt ccaagtaact gaaagttact
     2821 tttgttattt aacatttagg tcaattttag acagtttttg ttcaaaaagc gatatttata
     2881 gtggacggta ttactgctat attaaggata taaaatgaaa gcgtttaaaa aggtggcgta
     2941 aagatgatta ttaaagaaga gatttttgta cctaaagaat tagtgaaaga aaaaattgac
     3001 ctgctcgttg aaaacctgac aacgatcaaa gacgataatg gtgaattttt gttggacttt
     3061 gatggactga aagtagatga caaaagctgg acagtgtgga attggccaca aggtgtcggt
     3121 ctatatggta tttataaaaa ttaccagatg acaaaaaatc ctcgcgctta tcaagtggtc
     3181 aatgaatggt ttgaggatcg tatggaagaa ggcgctccgc ccaaaaacgt caatacaatg
     3241 gcaccgctat tgactatggc ttacttgtat gaagacacga aagattcacg ctatttgcct
     3301 tatttagaac aatgggcaga atgggtcatg aatgacatgc ctcggacaaa tgaagacggg
     3361 ttacagcatg ccacatatgg accggaaaat aagaatcagt tgtgggacga tacattgatg
     3421 atgacagtgc ttcctttggc aaaaatcggg aaactgttga atcgcccaga atatttagaa
     3481 gaagctcgtt accagtttat gatccatatc aaatacttga tggataaacg aacaggattg
     3541 tggtatcacg gctggacgtt tgaaggaaat cataactatg ccgaagcatt ttgggctcgt
     3601 ggaaactgct ggttgacgat tgctattcca gagatcattg aaatcttaga attatcgaaa
     3661 gaagatgcat tgcgcaaatt attgatcgaa acacttgaag cacaagtgcg tgcattaaaa
     3721 acttatcagt ctgaaagcgg tttatggcat acgttgttgg atgatccttc ttcttacgta
     3781 gaatcttctg caactgcagg atttgcttac ggtattttga aagcggtcca taaacgttat
     3841 ttaccacaag aatataaaga agtcgcttat cgtgctatcc aaggattgct tgaacaaatc
     3901 gatgaaaaag gagaagttca aaacgtttca atcgggacag gaatggggga cagtttagat
     3961 ttttatcgga atatcggaat cacagcaatg ccttatggac agtcgttaac ggtgctttgt
     4021 ttgacagaat tgctcgtctc ttattgttaa aagaaaggaa acccatatga aaaaaacatc
     4081 actgctgttt gcagcaagtt cgcttagcct aggtctgcta ggtctaagcg gctgcggaaa
     4141 taattctagt gtggcaactg tcgcagatgc agacgtaaat gagatagtta ccttgcgctt
     4201 tgcttatgca agcaacagcc agtaattgat gcaatgaatg aatttgggcg tttagtaaaa
     4261 gaaaaaacag atggttccgt acagatccag tatttcccag atggacaact gggtggagaa
     4321 cgtgaactga tcgaattgac acagtcagga gcagtagatt tcacaaaagt aagtgcctca
     4381 gcgttggaaa gtttctctaa agattattcg attttttccg ttccttatct attcaatgat
     4441 gaagaacatt tctttaaggt gatggataat gaagaaataa tggagccaat ctaccaatcg
     4501 accaaaaatt taggatttac cggattgacc tattatgatt ctggtcagcg cagcttttat
     4561 atggtagacg gtccgatcca tacgccggaa gatttgaagg gaaagaagat ccgtgtgatg
     4621 caaagcgaaa ccgctatcaa aatggtagag cttttaggcg gttcgcccgt acctatggga
     4681 agtgatgaag tgtacacgtc tcttcaatcc aatttgatca acggatcaga aaacaatgaa
     4741 tttgtcttgt atacagctgg acatggcggg gtagctaaat actattccta tgatgagcat
     4801 acacgtgtgc cagatatcat catcatgaat gatgccatca aagaacagct aactgatgaa
     4861 caggaaaaag ccattgaaga agcagcgaaa gaatcaacgg tttttgaaat tgaagctttt
     4921 gcaaaagcaa ttgaagaaga aaaagcttta gcacaagaag aacatggagt ccaattcaat
     4981 gatgtagata atacaccgtt tcgagaggca gttgccccac ttcatgatat attcaaaaac
     5041 gatcctacct acaaagacct ttatcaaaag atccaagagc aaggagcgta gctgtaatga
     5101 aaatgatttt aaacaaggta ttagagatcc tgggagctgt tttgcttgtt gggatggtcg
     5161 ctgttgtttt atggcaagta ttttccagaa cggttttagg aaatccaaat acggtcacgg
     5221 aagagcttgt tcgattcggt cttgtctggt ttgccatgtt gtcttccgcg tatgttgtgg
     5281 gacaaaaagc acgtttagcc gtaacggttt taagtgaaaa attaactggc aaaaaaagta
     5341 aaattttaga agttgttgtt caaagtttat ttctggtatt tgctgcgatg atcatggtgt
     5401 atggcggatg gaatgctgtc acattgacaa tggggcagat ctcgccttct ttaagtattc
     5461 ctatgggata tgtttatctg tcagtacctg tatcgggggt actcatcatt atctatagcc
     5521 tgatcaatct aatcgataca ttaagcaaca agccagcaat tcaagaaaaa atataaaaat
     5581 ggaggaataa aatggcaata gaagccggat tagtgttatt ttttgtattt gttgtgttgc
     5641 ttgtattagg gatgccgatt gctataagta ttgcattctc atcgatgatg actttattgt
     5701 tagttatacc gtttgatgtt tctgctttta gttcagctca aaaaatggtg ggaagtatca
     5761 atagtttttc gctgatcgct attccgtttt tcgttctctc gggcattatc atgaataatg
     5821 gagggatagc taagaaacta gtggattttg cgatgttgtt cgtcgggcgc gtgccggggg
     5881 ctttggctca tacgaatgtg ttagggaatg ctttatttgg ttccatctct agctcggcca
     5941 ttgccgcttc taccgcgatt ggtggagtgt tgatccctca gcaagtggaa gaaggatatg
     6001 accggaagtt tgcgacagcg gtcaatatcg cgtcagctcc gacaggtatg gtcattcctc
     6061 caagtactgc ttttatcatg tattcacttg ttgctggtgg tgcttcgatc tctgctttgt
     6121 ttatgggagg ttatctcgta ggcgctt
//
LOCUS       DAZUMV010000139         6068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000139 DAZUMV010000000
VERSION     DAZUMV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.142"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACG8FK_002468"
     CDS             832..1566
                     /locus_tag="ACG8FK_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5098025.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACG8FK_002469"
     CDS             1582..2751
                     /locus_tag="ACG8FK_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5098026.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8FK_002470"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8FK_002470"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5098027.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACG8FK_002471"
     CDS             3841..4767
                     /locus_tag="ACG8FK_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5098028.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACG8FK_002472"
     CDS             4786..6036
                     /locus_tag="ACG8FK_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5098029.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2026 a          997 c         1247 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAZUMV010000140         6060 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000140 DAZUMV010000000
VERSION     DAZUMV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.143"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1088
                     /gene="yidC"
                     /locus_tag="ACG8FK_002473"
     CDS             138..1088
                     /gene="yidC"
                     /locus_tag="ACG8FK_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS5098030.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1267..2241)
                     /gene="lacD"
                     /locus_tag="ACG8FK_002474"
     CDS             complement(1267..2241)
                     /gene="lacD"
                     /locus_tag="ACG8FK_002474"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5098031.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(2238..2696)
                     /locus_tag="ACG8FK_002475"
     CDS             complement(2238..2696)
                     /locus_tag="ACG8FK_002475"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5098032.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(2693..4156)
                     /locus_tag="ACG8FK_002476"
     CDS             complement(2693..4156)
                     /locus_tag="ACG8FK_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5098033.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(4138..5088)
                     /gene="pfkB"
                     /locus_tag="ACG8FK_002477"
     CDS             complement(4138..5088)
                     /gene="pfkB"
                     /locus_tag="ACG8FK_002477"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5098034.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            5231..5979
                     /locus_tag="ACG8FK_002478"
                     /pseudo
     CDS             5231..5979
                     /locus_tag="ACG8FK_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
BASE COUNT         1843 a         1327 c         1039 g         1851 t
ORIGIN      
        1 tttttgtctt ttttcaaaat ttttcgctac gaaaaagaaa gaatatttat atgaaatttc
       61 tatgaagttg attgaattcc atgcgctttt ttagtatagt taagtttgtg taaaaattat
      121 agaagaggaa gtattctatg aataaatgga aaaactggtt attgggatcc ggacttgtca
      181 gtatcctgct ctttttatct ggctgtgtgc ggatggattc aaacggaaac cctgacactt
      241 caggaatcgt ttatcgagta ctcgtccatc ctatgggcca agcgattaca tatctagttg
      301 agaatttcaa ttggtcttac ggctgggcag ttattgcaat gactgtgatc gttcgtatca
      361 tcatcttgcc actaggtatc agtcaatcga aaaaaacaat gatccaatct gaaaaaatgc
      421 aagcgttaaa accacaagtt gaagcagcac aacaaaagct aaaagctgca acaacacgtg
      481 aagaacaaat ggcagcacaa gcagaaatgc agcaagtata tcgcgaaaac ggattgagta
      541 tgaccggcgg aatcggctgt ctgcctctat tgatccaaat gccgatcttc tctgccttgt
      601 attttacggc tcgctacaca gaaggtatcc gtgaatcatc tttttacggc atcgatctag
      661 gctcaccaag tatggtgctt gtggccattg caggggtcgc ttatctgctt caaggatata
      721 tttcaacaat cggtattcct gaagaacaga aaaagacgat gcgtacgatg ctgatcgtct
      781 ctcctgctat gatcgtcttc atgtcgatca gtgcgccagc cggcgttaca ctttactggg
      841 tagtcggtgg tgtgttcagc tgtctgcaaa cattcatcac aaacgttatg atgaaaccac
      901 gcttgaaagc acaagtggca gaagagttga aacaaaaccc gccaaaacaa gtcgtcactc
      961 caagaaaaga cgtgacagaa tcagcaaaac cagctgacaa caaacaagca acaaaacaaa
     1021 tcaaacaacc aaaaaacagt acaaataaca aaggccgcaa cgcaggaaag cagcagaagc
     1081 ggaagtagat agtaaataag agtgtaataa aaattcatta acagttttag caattaaaaa
     1141 tatcgaccaa gatttttgaa aaagaaatct agtcgatatt tttatttaag tgaatgtata
     1201 atttgaatca tcgattgatc gctatctaat tgcagcagca tctttttatg aagaaacaac
     1261 tttttcttat ctatcaacgt agacaggtgt tgctgtttct tttaatagtg tacttaattc
     1321 cttaatattt tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt
     1381 aacaaaaggt gccactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt
     1441 cgaacctgat tgttttgcaa aacgtaatgt atcttgaaat aacgtagcat tgactcccgc
     1501 acttaaaaaa ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc
     1561 ctctgcttga ctataacaag tctcttgccc atatccttcc acaaatctca tattcactgg
     1621 cacttctact ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc
     1681 aataacttta tggggtttta cttttgcata ggcaagagag gacggatcgc caatagttgc
     1741 atcataagaa aggatctcta aaaagaaagg tatgccttct gcatcgcatt ctgaaccaat
     1801 ccgttctata actatttgtt tttgcacatt aatctctgct ggttcatcca cgtcatagta
     1861 caataaaaat ttacaagcat ccgcaccagc ttcttttaac cttttcaccg accaaatatt
     1921 caataaatca ggtaatcttc ccggcatttt cgtatcatag ccggtttttt cataagctag
     1981 cagcaagcca cattctttct ctctaagcgc tgctgcaggc aggccgcatt ctggatcaag
     2041 taaaatagct gaggcatagg gagtcaggta ttgagaaacc atctgtttaa attggatgat
     2101 gtcttctgat gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgctggtc
     2161 gattgctaac gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgctt
     2221 ttctatactc atcttcttca tagatcttca cctatttctt tagttaatat tgtggctatt
     2281 tctgtttttg attttgcttt tctcaactgt tctgtaacct cttttcgcat caatgatcgt
     2341 gcaacgagag acaataattt caaatgagtc gttcctgctt ctgaatctgg aataaataac
     2401 gcaaaaataa aatcaactga ctgtctatct aaactgttcc attcaattcc tttgttcagt
     2461 gtgacgataa tcatactagg ttcgttgatt gtttgagact tcgcatgagg gatagcaaaa
     2521 cctgacatta ttcctgtagt accttctttt tctctttcta gaagttttcg atatacttct
     2581 ttttcgtcat gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct
     2641 tcttgactat ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata
     2701 agcctcctgc tcttccttct tcgataactg tacgatttgc tgctttatta tccatcttct
     2761 tgtctttatc agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg
     2821 aaccaataag aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa
     2881 aaccgccatg tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg
     2941 atgagcctaa catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag
     3001 ctccctccgt aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat
     3061 tagtatcaaa atatttacca aacaataaag ttgcaaaacc agtaatcaac ggtggtgcaa
     3121 tgcaagctac tgacacgcca gccataaaag tcgtgtttcc ttgtgccaaa agagctgtac
     3181 ctgttacata tgccgcctta ttaatcggtc ctcccatatc aaacgcacac atgcaaccaa
     3241 caatcacgcc aagaacaagt ggattcgaat gttcaaatcc agctaaaaag ctcatcattc
     3301 cttcatttat agccttcatt ggacccgcca gcaaagacat cacgatgccg ataaacacta
     3361 caccaataac tggatataag aagattgcct tcaagccatc tagttgttta ggtaaaattg
     3421 tcagtagctt ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaataccta
     3481 ctaaaaatcc agtaccgcca ctcatggcca cgattccacc aacaaatcca acgactaacc
     3541 ctggacgttt cgcgatccct tctgcaatat aagctgctaa aactggaacc atcatcccca
     3601 ttgctgcatc gccaaccgtt tttaacatcg ccgctgtagc attgtattgt gcatggtctg
     3661 gatcaaaaga atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta
     3721 ccacgaaagg caacatatga gagacgccat tcattaaatt tttatagatg ctgcgaccaa
     3781 aattagcacc acttatcgtt tctgattcta aagtatcaac agcttcatta tctgctaaga
     3841 tggtcccttt tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac
     3901 tgacagaaac atcaatgact cgttttcccg caaatctttc tgaatggaca tctttgtctg
     3961 cggcaataat gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc
     4021 caatttgtcc atgggtctcc actttgatcg tgatgccttt ttttctagct gcttgctcta
     4081 atgcctcttg cgccatgtag gtgtgtgcaa ttcctgtcgg acaaccggtt gctgcaatta
     4141 gttgatattt tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc
     4201 ctccacatcc aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat
     4261 gcttttacgt aacgtttcat caagcggtcg tcgattcatg tatcctacta aaaaagtgcc
     4321 taacatggca tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg
     4381 aatcaccctg tctttcgtaa aaagaatcgc tcctttctca ccaagggata acaaaacatt
     4441 ttctgcacct gactctacca gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat
     4501 ttttcgtccg aaccaagaag aaagttcttc ctcattcggt ttcagcaaaa atggtcgata
     4561 gggcaaacaa tccatgacct cttgatcact gctatcaata atcaaatgaa tctccttttc
     4621 ctgacaaatc cgactgattt ccactaaaac cttcggagac attcctcttg gtaaactccc
     4681 agaaacacaa agataatcct ctgcttgtaa tgctcttatt tgatttaaaa aattatggac
     4741 agctgcctgc ggtatttcag gtccttgatt cactaatttg tattcttctc tcgtttgatt
     4801 gacttgtgta aatacattga tacgcgtcat accaggcact tcaataaact ctgtttggat
     4861 ttgtttcttc tgtaaatagt cttctatata ttttcctgtg aaaccagcag aaaaacctaa
     4921 tgccgtactt gggaaatcta gcatctttaa tactagtgac acatttactc cttttccgtt
     4981 tgcctgaatg tcatcctcct ttgtacggtt taccataaaa ggatgcatct cctctgtttc
     5041 aataaataga tcaattgcca aattcatcgt gcatgtgtag atagacataa ccaatgacct
     5101 ccttgaatct attatgaaat acgcgaaaga gaaaatgttt tcttaatcgt tccaacacaa
     5161 agaaaacctt tacatttata aacacatcgt gctatttttt aactaacttt tagtgaaaga
     5221 aggaaagaat atggatcaca aattaagtga aacagaacag tacctctgga actttatcga
     5281 acaccacatc ctagagatcc ctaactactc catcgtgaaa ctaagtgaac aagcaaatgt
     5341 ctcaacagcc accattgtgc ggacaatgaa gaaaaaaggc tatgaaggat ttacttcgtt
     5401 taagcaccat ctaaaagaag gagaaaacaa gaacatcaat ttttcttttt agataaagta
     5461 gacaaaggta tccgtcgagc cattctaaaa aatgaacaag aagtggtacg tacgatcaat
     5521 atgttggaaa tcgggaacat tgaagatgca atacaaagaa tcaaatttac tgaccgggtt
     5581 tttgtctttg cccgaggatt ttcagaaatg attggtcaag agatgctcgt aaaacttcaa
     5641 ttaacaggaa aaaattgtca aatgcatact gatccagaga ttatcaaaag catcagtcgg
     5701 aaactaacta agaaagacag cgttattttt gtttctttga acggcgagac aaaagaactc
     5761 gtcaccgccg caaagaactg ctatgatgca gagattggtt cgattctagt tacagccaat
     5821 cattcctcta cactcaaaga ttattgcgag ataaatctcg taggtttcaa atcagaaggt
     5881 tcttatttcc ctgattatga agtacgttca cgcttgcctc tccagatcat cgcgagaatc
     5941 ctgcttgacg cctatgtatt gcgtatgagg gagaattgag aagcagttta ttatggttgg
     6001 ttacagttta aaacatcaca ttttttattc tttatattgt atggactcac gtaaactttt
//
LOCUS       DAZUMV010000141         6023 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000141 DAZUMV010000000
VERSION     DAZUMV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.144"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..2041
                     /locus_tag="ACG8FK_002479"
     CDS             134..2041
                     /locus_tag="ACG8FK_002479"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS5098035.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(2582..5860)
                     /locus_tag="ACG8FK_002480"
     CDS             complement(2582..5860)
                     /locus_tag="ACG8FK_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS5098036.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         1752 a         1226 c         1137 g         1908 t
ORIGIN      
        1 tcattataag tgaaggattg aaaaaaacaa atcagtttga ttgttcgaat tttcaatttt
       61 ttatgttcta tttgttaatt ccttcatttt gtaagataat gggcacacga aacaaaaaag
      121 gagcgatcga tgaatgaaca aagaacgatt gaaacaagat ttctatgaag cagtcaatga
      181 agaatggcaa aaaacagcaa aaatccctgc agataaacca gctacaggcg gtttccaaga
      241 tttagttgac gggatcgacc aacttttgat gggagaaaca gacgatctgc ttgcccatcc
      301 agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca tattatcaac tggcaaacga
      361 ttatgaccga agaaatgagt tgcatgctca accgcttcaa cctataatcg aaaaagttga
      421 atcattgcag tctttcgacg aattgaataa gcaatttcca gaatggctgt tggatacatt
      481 accattgcca tttaatctgg atgtagatgc agatatgaag aatgcgcaaa cgaacgcctt
      541 ctttgcttat ccgccttctt tattccttcc agataagacg tattatgcat cagaccatcc
      601 caacggtgca caactaatag gcgttttctt tgatatggct gttcagttat tagagcttgc
      661 cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa gcagtgcaat ttgataaatt
      721 gattgctcct catgtaaaaa gtgcagaaga aaacgcagat tacagcaaaa tgtataatcc
      781 gcaatctttt gatacattta ttaaatacac agataaactg gacttgaaaa aacttgtcac
      841 gggtttgatt gggacaactc cagataaagt gatcgtaact gatccagtgt acttcgatca
      901 tttgaatgac atcctgacag aagctcattt tccgttattc aaaagctgga tgatcattaa
      961 aacagtacgt tctcttagtg gttatctatc agaagaattc cgccaagtca gcggaatcta
     1021 ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag aaaaaagcag cgtattacct
     1081 tgcctctgga caattcgatc aagtagttgg cgattactac ggaaaaaaat attttggcga
     1141 agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg atcgccgttt ataaaaaacg
     1201 tctagaagaa aatgactggc ttggggatgc aacaaaagaa aaagccgtca taaaattaaa
     1261 taacttaggt atccaagtag gctaccccga taagatccca gcattattcg accagttcaa
     1321 aacagtacct gcagatcagg gtggtacttt gctttcaaat actcttgttt tcagcaaact
     1381 cacattaaaa gatcgtttca gcaaatggaa caaaccagtg gatcgttcag aatgggaaat
     1441 gagtgctgac acggtcaatg cttattatca tccatttcgg aatgtgatcg tctttccggc
     1501 agcgatcctt caagcaccat tttacagtct agaacaatca agctctgcta attatggtgg
     1561 aatcggagct gttatcgctc atgagatttc ccatgcattt gataataacg gcgctttatt
     1621 tgacgaatat ggcaacttga acaactggtg gacagaagaa gatcttacac aatttcaaaa
     1681 caaagcacaa gcaatgatcg aagaattcga tggtctgcct tttgctgacg gaaaagtaaa
     1741 cggaaaatta accgtttcgg aaaatattgc tgatgcaggc ggtctaagct gtgcattgga
     1801 agcagcaaaa acggaatcag atctttctat cgaggaattc tttgtcaatt gggcaacgat
     1861 ttggcgtaca aaagcaaaaa aagaatacca acaactttta ctgcaaatcg atgtccatgc
     1921 ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt gacgaattct atgaaacatt
     1981 tggtatcacg gaacaagacg ctatgtatct agcgccagaa aaaagagtgc gaatctggta
     2041 agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa ggaatttggg aaaatcgctt
     2101 ctcaaatttt tgtgacattt cggcttattt ttcgaagaag ttacttctgg aataccatgt
     2161 attagaggtt gtgaaaaagc tgcttagttt caagtagtaa gaaaaatttt gtgaaaatgg
     2221 ctttccacat ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt
     2281 tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt agagaaaaaa acggaaaaat
     2341 agttcctcat attttgccgt ttgttgagct aataccgagg gtcagctgat tgaacaccgt
     2401 ttagtggaga gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaatcaaaa
     2461 gcagcaagga gaacctcatc tataaaaaga tagaaggaag tgagacatct attggtctca
     2521 tttccttcta tttttacttt cctcagtcaa ttgttttaca tcataaaagg agaaattcca
     2581 cttatttttt tcgctgtttc atgacataaa caactacacc gattaacaaa attcctccag
     2641 ctaatgctag caacgggtta ttaaattctc ccgtttttgg gagtccttga gtgccatttt
     2701 tttcactttc ttttgctgtc ctatccggat gagaaggttt tccatttggt tttccatttg
     2761 gtgattcatt tcctggattt tctctttctg gtttgtcatt tcccgggggc acatcggcag
     2821 tttctcgcac agtgatcgct ttttgctggg taacagattc tgtatgagca gaccattttt
     2881 ccccatcaaa tatttccgaa cgataagtga cttctagcgt atattctcct tttgaaggtg
     2941 ctgaaaaaat agcggtatga ttagaaaatt gagcacttga ctgtgtttca acgatttgga
     3001 tcttttctgg gaaataacgc atatcttcaa agacaggcgt tccttgattt ggtgtgaaag
     3061 ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt tccttctgca ttctccgcca
     3121 caccatttgc ttcttgtata ataatcggat tattttgttc atttggtttt gtctgatttg
     3181 gttcttgtac gattgcatac tctggatatt ccgaagacgg tgaggtttct ccgtcaaaaa
     3241 tagcacctac agataatgga agaccatcca ctctttcctt ttctgtttga cgattccccg
     3301 gtttaatctg catcagtcca tgaacatgga tacttccatt ttcatagcgc gtaacaggct
     3361 gctgaccttc tttcgaacat gtatttagag actcgatcca atcttttgat atggtatgtc
     3421 caagagtatt ttctgattgt ttctctcgtt cattccaata gtagttcaac gagtggtaga
     3481 tttgatcttc gttttgattc aatgggcgga tctgctctct catctcaaga ttctctgttc
     3541 ggaaagataa gataccagaa gcatggtagt ctgtgtttcc tgctgaagag gtataaagag
     3601 ccacattata tgaaccgtta ttaaaacttg tactgctatt gacgataata tctgttccgc
     3661 tattggaatc gattcctttc gcaagatttt cgaaagacaa actattgatc aattcatgag
     3721 ggcccgttaa agaagaaccg cccattttga atccattacc gttaccttct ccctcaattc
     3781 ctggtaccca gccgtttcga taagcaactg agtttttgat gattactttt ccgatcgaac
     3841 ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc attatgatac gcgatacaac
     3901 cgtcaaatac atttccttct cctattgtca gtttggccgc aaatccatcg gcatcttcaa
     3961 atcctggatc ggcattttca aaagatgtac agttcttgac taagttatgc gcgggccatg
     4021 cttcgaaagg atcatttgag ctaccgctga tttggattcc tgtatttcca ttccgataag
     4081 catgaatctc ttcgatcaaa ttatgatttc cagaaatctg taatcctttt tgcatagctg
     4141 cactatttgt aatgtcaaac cctcgtaaac cccagtagtg actgaacagt gtcatcccat
     4201 ttccagttcc tttaaaatca agtacaggac gcttgttttc tggatttttc tctgcaatca
     4261 actgaatcgg gttttctctt gtcccgttaa cgccttttgg aatcgtcaat cctctttcga
     4321 aggaataagt tccaggcgcc atgacgattg tctgcccagc atatgcgtat ctcaatgctt
     4381 gtgtcagaga taatggattt tgtctgcttc cgtttcctgc atctgaccct tctggtgtta
     4441 cataaactgt cgaatattta ggtttacggt agtccactgt atgaagaatc tcttttgttt
     4501 cataactagt catctctacg tattcttctg gcggatagtt tttatctggc gtaaaactga
     4561 aagtaaattc gttcgttcct tccatcagtt ttgtctgata atcaaattcc cgattcgcag
     4621 tgatagccaa gttttttgcg ttcttaagca cctctcctgt tgctttattt ttaattgtca
     4681 ataatccgtc agcatttgct ctaaaggaca tagtatgatc ttggtttcct gaatgattac
     4741 tagaggtcac agtgtaaatc ggatcaatca agcgagtagg tctttcttgc ccaggaggat
     4801 ctgtcgcagg atctgagtaa gcaacttcga ttccttctac cttgatcgtc atattccttg
     4861 ctgcagcaaa tcctgcataa ataaccgatt catctgaagc gtataatttt tcccagtcat
     4921 acatgatgtc agaagccatt tcttctcctg tttcaggatc gtaataatat gcctctaaac
     4981 cagtattcgt ttttttcatc tctacgtcca gtttagtaat cgagttcgtg gccggaaaca
     5041 gattcccatg accgtttccg aatatattat aagaacctgt ttctttttcc aagaaccctg
     5101 ctgaagtctc taaaggtgtt gtttccactg agacactccc aggagctggc gcagcttgcg
     5161 gatcttcaga tgatattcct gtgattgccc gtgtccccaa tccaagtcgc atcgtatatt
     5221 tgctgccgct cgtgttgacc acttcttgtc tatccgagtc ccagacatat tcgatccgac
     5281 tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc aaacaaatga ttttttggta
     5341 ctgcgtctct taccattaaa gcaaatcctt cttgggcatt cgtataggtc caagattcta
     5401 tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg gattgcttga taataatagg
     5461 tcattccatc tgatccgctt gactggaatt ttccgccgtt attcgtggaa gagagccgca
     5521 cgccgtcttc taatggttca atggcatacc tactagcact tgtgctttct ccgaaaacaa
     5581 cccctttcca ctcttcattt gcttcttcag attgcttttt tgtcttttca ttttctgatt
     5641 ctttcatcat aggatcattt ccttcttccg tatccatatc ttcactagtt cttggtattt
     5701 cctccattgt tgacatccct tcaatgttca cgtgtgcatt ttcttcgttc acagactgtt
     5761 gttcgttagc agcaacttca acgacagaaa ttaaaaccgg ggtactgagc ataaggagat
     5821 tcatcattag cgcactgtat tttatacgtt tttttaacaa gtgtattcct cccgtaacaa
     5881 attagaaagc gtttacttaa agtattacct aatttatttt tactgtcaat aaaaaacaaa
     5941 attataataa gtgtatttta taaaaagaaa aaatataact taagttgttg ataataaaga
     6001 gatttattaa cgtaaaaaga ata
//
LOCUS       DAZUMV010000142         5977 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000142 DAZUMV010000000
VERSION     DAZUMV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.145"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /locus_tag="ACG8FK_002481"
     CDS             complement(<1..450)
                     /locus_tag="ACG8FK_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5098037.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="ACG8FK_002482"
     CDS             674..1465
                     /locus_tag="ACG8FK_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5098038.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="ACG8FK_002483"
     CDS             1431..2384
                     /locus_tag="ACG8FK_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFS5098039.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="ACG8FK_002484"
     CDS             complement(2782..3756)
                     /locus_tag="ACG8FK_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5098040.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="ACG8FK_002485"
     CDS             3871..4305
                     /locus_tag="ACG8FK_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5098041.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="ACG8FK_002486"
     CDS             complement(4430..5452)
                     /locus_tag="ACG8FK_002486"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFS5098042.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5844..>5977
                     /locus_tag="ACG8FK_002487"
     CDS             5844..>5977
                     /locus_tag="ACG8FK_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFS5098043.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     P"
BASE COUNT         1877 a         1128 c         1030 g         1942 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatcc
//
LOCUS       DAZUMV010000143         5871 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000143 DAZUMV010000000
VERSION     DAZUMV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.146"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(604..1644)
                     /locus_tag="ACG8FK_002488"
     CDS             complement(604..1644)
                     /locus_tag="ACG8FK_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5098044.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1993..2319)
                     /locus_tag="ACG8FK_002489"
     CDS             complement(1993..2319)
                     /locus_tag="ACG8FK_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098045.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2306..3094)
                     /locus_tag="ACG8FK_002490"
     CDS             complement(2306..3094)
                     /locus_tag="ACG8FK_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5098046.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3462..3752)
                     /locus_tag="ACG8FK_002491"
     CDS             complement(3462..3752)
                     /locus_tag="ACG8FK_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS5098047.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3888..4067)
                     /locus_tag="ACG8FK_002492"
     CDS             complement(3888..4067)
                     /locus_tag="ACG8FK_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098048.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4064..4414)
                     /locus_tag="ACG8FK_002493"
     CDS             complement(4064..4414)
                     /locus_tag="ACG8FK_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098049.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4407..5732)
                     /locus_tag="ACG8FK_002494"
     CDS             complement(4407..5732)
                     /locus_tag="ACG8FK_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5098050.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
BASE COUNT         1749 a         1064 c          853 g         2205 t
ORIGIN      
        1 gtaagggatt ttattcttta tttaaaactt tgcaacagaa ccgtccgttg cgggtaatac
       61 cgtaacactg aagggcacgc attaaggcgt catcttaatg ctttcctgta ggacactgac
      121 ggagattacc agtctcccga gccttacagg ggcctttctt ttttttgtat tttcaattat
      181 cacaatttat cgtccaaaag tgcagaacga aaatcaattc atcacaagca tatcagacaa
      241 ttaaatttaa aacaactaaa aatatacaaa aaagtgtaat tgaatattag cagcactgcc
      301 tgaaaagaag taatatcagg cttttcagct tggggaaata agtgaattac ctctcttcat
      361 gaattattat aactcaaaaa agcacatatg caaacactag ttccctattt ttttgaatga
      421 aaaataacta aaatatctaa cgattatgac tttaaaacct acgtttgtga ttgttttcat
      481 aaacttttaa tctaaaaact ccggaataat agctatttat ttgtaacatt tgtaataaaa
      541 ttaaaatcat aaaaaagttc taatttaaaa ttgatcattt ttatagtgat cgctttttat
      601 caactactct agccaatttt ccgtactaac tgttggtaat tcgttaaatg tttctctttg
      661 ttcgtttgca agctttgccg caatagcttt ttcgacaaac caatttttta cataaacgaa
      721 aattagattc tgcaaattgt ttactttttc agttttttgt ttttgataag ctttcagcaa
      781 agtcatatat aatcctttgt cagcatcaat tccataagat tccagttctt cataaacaac
      841 aactgtttgc tctaattctt cagctttttt ctttgcatta tgaatagttt ggacggtttt
      901 ttgagcgtct tcgtaagtac tagaaaatgt ttttatcaag tttaatacat tttctggtac
      961 aaatgtattt atactgtcct ctttcatgat gtcgacgaag ttatctagta aaacttgatt
     1021 ttgtaactga tcagtttcag tgtctattat gtgtctatta gtgtctatag aattagaata
     1081 gaatagattg tctgcgattt tcgcaattac ggttgagtcc ttgatatgac tggattttgc
     1141 cgtatttgcg attttcgcaa ttacggtggt atcattgatt tctcgcggtt ttgccgtatt
     1201 tgcgattttc gcaattacgg tggtatcagt attgtcttcc tcttccatga ttttaaagac
     1261 gtcttgattc gttacttctg gcttgcctaa gtacaataaa ttaggcatgg attcaatctt
     1321 accgtcacgt ttatttactc ttcctctctt ctgtttcaga agacctgctt tttctaattc
     1381 ctttttgatt ttagatactt taccttcgcg gcattgtaac aatttcatta attcttcaac
     1441 tgtgaatatg aaaaatatat tatcatcaga atctacccag ttatttttga ctgaataatc
     1501 aaatcgatct ttaagtagca tataagcaat ttttgcatca tttgatagat ccatgtattt
     1561 ttcgttagta aaaaatacct taggcatttg ataatatttt tcacgataaa tttcttttga
     1621 tttgtaataa ttaaaatcat tcatagccaa tcctctttct gtaggatagc gttttgttca
     1681 aaactatgtt acaatgaatt aaacaaatat atctatccac tatatatttt tctactgctg
     1741 ataagtcgcc aaactttgtt aaagcagtag tcttttttta tcttttttta gtttacctta
     1801 aaaaataaat taaagcaaac cgctctattt caaggtttaa gctcatagta actatatcac
     1861 gatattacaa taatattata tcacgatatc gtaatattac aatatgaaga taagcgactt
     1921 atcagctttg atactactga tttttataaa aaaaatatca cgatattatg atatcgtgat
     1981 attgaatgta aattaaattt tattttgttt ctttgctagt ttatatcctt caatcaagta
     2041 ttcttcaggt atggacttta atagcgcatc ttctataacg tctggtttac ttttgtctga
     2101 ttcagcaata gtcgtccaat atttgagtgc tgacattaga attggattta tacgaatgct
     2161 atctgctgtt cttacactac ttccttgatc ttttatgact ttacttatat ttttcttggt
     2221 taattcgatt tcttctactt cttttgattt aaaattcttt ttatttttat ttcctagtaa
     2281 tctacttact gcagcttgac tgcgattatc ttctgccatc aaaatcacca acttctttta
     2341 aatattcttc tgtaaatgct ttatagtcat cttcagctgt tgagggttta ttaaaattga
     2401 ttagatctga tccagcagct tgcgcttcgt caacggttat tgtcctacga atataagttt
     2461 tgaaaatttt tgcaccaatg actctagaaa tttcggttgc tgcagacgtc atttctttag
     2521 aaatgttata acgcttatta tacttagtaa atagaactcc tcgtacttca agcttgtcgt
     2581 tcgtatattc ttttaccgtt tcaatagttt cacttaactt tattagtcct tttaatgaga
     2641 aatacgcagc ttcaactgga atgataattt catcagaaac agtaaatgcg ttgactgtaa
     2701 gcattcctaa acttggtgga caatcaatta taatataatc atagttgtcc ttgattggtt
     2761 gaagtactct tttaagcctg tgttcacttc ctacttgagt aaattccctc tctgcagaag
     2821 aaagggctat gtcggctggc aaaacatcaa ttcctttata gtccagaata gcatctgtaa
     2881 tgtcagcctc cccttttaat acttcataaa ttgtaggttg gtttgttcca tctatatcga
     2941 aacctacatt atctgtagca tttccctgag gatcaatgtc cactaaaagt acatttttac
     3001 cgtaaacaaa tttcaaacat gctgctaaat taacgctagt cgtagtttta gaaactccac
     3061 ctttttgatt ggctactgac aatattcttg ccattaaaat atcccccatt catcttatat
     3121 tataatataa ttatatcata atattgtgat attgcaatat cgtatatgca ttatattgta
     3181 atattgtgtg attattttgt tttaaagata tatatcggtt cggatataaa gattattcat
     3241 atgttattcg aaattaaaat aatcactttt tattacttta aaatttttta ttaatgataa
     3301 gaaatatgtt ttgcgagcag caactagaat aatttaaaca ttactaaact gtctatattg
     3361 ttactaataa ttcccttctt atagtgcatt ttcttagaaa aaaacaatat tacgatatca
     3421 tgatattaag atatcgtaat attatacaat tgtgattgat attatatatg tttttttatt
     3481 tttttatcat gtttaatctt gaaggaatca tattcttttt ctaaagacag gagcttttca
     3541 taaacatcag acatgtttaa actagaggcc aggcttaaat cataaacgaa tgaagcaggt
     3601 aatgatttta ttctacgttg tcgggttatc cagcttgcaa ccgttccttg tgggtaggca
     3661 tataactcac aaaattgttc tacagtgatt cctaaattct tgataatgta aacattgatc
     3721 gggtgcgagt aaacaaatgt attgatagac atggtgcgta agcctacttt cttttattat
     3781 tgcgtttgca ataatagttt aacacacgca ttattctagt atctactcct atttgtaaaa
     3841 acaaaaaagg attcatagag ataactctat agaatccgta aaatacctta tatatataac
     3901 gggaagatat aaaacctaaa attatgataa cctgctgcag cttctacata gaaagagaag
     3961 cgctcaacac caacctcatt tatcaaacgt tggccataac cccaaatctc ttctgatagc
     4021 tgatcaaagg ttatatactt tagctcatat tgctggataa tgttcattct aatcgactcc
     4081 atttattatc ctggtaaaat tcgatatgac gtatttcgtc gtagtgaatt ttgtcttttc
     4141 caacgtagat ccccagctca tcactaccta agatcttgcc aatgatatct tctggatagt
     4201 atccttcagc gtttcgttct tctttttgaa tagatactga tctatttttt atgactgcag
     4261 cgttcaatac ttccgcaatc tcttcagtcg tcatttgttc ttttgctaaa ttttgactgg
     4321 ttcgttgttc attgtcggca tcaatttctt cagtatgatc agataaataa aaccctgccc
     4381 actttaaacg tccacgatca ttgtaatcaa tcatttttaa ttcccccatt tccgcccgca
     4441 tgaccaccaa caagtgtgct tctagtaatg cttctagcgc cttctaacat tgaagaagca
     4501 tgtacaatcg acgtaaaacc atatttttgc cgaatcttgt caataatttt atctaaattt
     4561 tcttcgtcaa tttgcttttg tggatcacta aataaatcca gctgaagcga atcggtgtac
     4621 acaagcttgc tataggttat tccaacatgc cttacttctt gtccttcata atacttatca
     4681 aacaagaaaa gacaataatt ctgcaattcc tttgtattat ctgtgatagg gatcttcatc
     4741 tggtgtgaaa accctggtct cgtttctaag atcgacgtgc caatgtgtaa atggacacaa
     4801 cctgttttac agttatgctg cctaatgcgt atcgctactt gttccgccat ttcttttacc
     4861 accagctcga tttcatttct tctagtataa tctcgtggta acacttgtga atttccatat
     4921 gatttttcct ttgtcggttc tggtgggatt gctatatctg ttcgatcgat cccattagca
     4981 tggaagtaca gctgtaagcc aatcacacct agtctattct taatcgtata aggattccag
     5041 ttagcaagat ctctaatgct cataatcccc atctgattga gtcttttttt cattcgagag
     5101 ccaattcccc aaaagtccgt catttcagga atattccata ctttttcagg aacgttttcg
     5161 tacgtccatt ccgcaacaaa tccttcatta tgctttgctt cgttgtctaa ggctagttta
     5221 gccaaaaggg gattatctcc gacacctact gtagcaatca atcctagctc ttctttaata
     5281 ttctcctgga tcatttgagc cagcttcttt ctccgttgac ttcgtgttcc ttcagtcgta
     5341 aaaagattca gtgatcgggt cacttttaag attgattcat cgatcgagta aatcagtaga
     5401 tcttcatcag ccacatatct tctgaaaata ttatttacct gcatatttcg cttgatatat
     5461 aggttcatac gtggtggaac aacgtgtagt gttttaggaa atggttgtgg taagtcacgt
     5521 ggtctactca catttgtaat accataccgc ttttttgctt caggagaaga agctaatatc
     5581 aatcctgaac cagtattatc agctcgactc ataacaacaa gttctgttgt taatggatct
     5641 aaatttcttt ctatacattc gacacttgcg taaaagcttt tcatatcgat taaaaagtaa
     5701 tcatttactg gctcttttga ataatccatc ataaataaca ctccattctt tcacattata
     5761 caaacatatg ttcgatattg taaagaagaa atagctaaat acataaaaaa agaagcatag
     5821 gtatgctatc accctgcttc ttcttttgat ttgtatgatc aatttattct a
//
LOCUS       DAZUMV010000144         5721 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000144 DAZUMV010000000
VERSION     DAZUMV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.147"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACG8FK_002495"
     CDS             196..921
                     /locus_tag="ACG8FK_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098051.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACG8FK_002496"
     CDS             911..1420
                     /locus_tag="ACG8FK_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098052.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACG8FK_002497"
     CDS             complement(1490..2938)
                     /locus_tag="ACG8FK_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5098053.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACG8FK_002498"
     CDS             complement(2938..3654)
                     /locus_tag="ACG8FK_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS5098054.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACG8FK_002499"
     CDS             complement(3635..3985)
                     /locus_tag="ACG8FK_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS5098055.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACG8FK_002500"
     CDS             4129..4902
                     /locus_tag="ACG8FK_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5098056.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8FK_002501"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8FK_002501"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5485..>5721)
                     /locus_tag="ACG8FK_002502"
     CDS             complement(5485..>5721)
                     /locus_tag="ACG8FK_002502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098057.1"
                     /translation="NLFEGTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEYV
                     LGISKCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT         1661 a         1124 c          994 g         1942 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt t
//
LOCUS       DAZUMV010000145         5685 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000145 DAZUMV010000000
VERSION     DAZUMV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.148"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..520)
                     /locus_tag="ACG8FK_002503"
                     /pseudo
     CDS             complement(1..520)
                     /locus_tag="ACG8FK_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            668..1809
                     /locus_tag="ACG8FK_002504"
                     /pseudo
     CDS             668..1809
                     /locus_tag="ACG8FK_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1935..3014)
                     /locus_tag="ACG8FK_002505"
     CDS             complement(1935..3014)
                     /locus_tag="ACG8FK_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5098058.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3117..3557)
                     /locus_tag="ACG8FK_002506"
     CDS             complement(3117..3557)
                     /locus_tag="ACG8FK_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5098059.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3614..4024)
                     /locus_tag="ACG8FK_002507"
     CDS             complement(3614..4024)
                     /locus_tag="ACG8FK_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5098060.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4204..4638)
                     /locus_tag="ACG8FK_002508"
     CDS             complement(4204..4638)
                     /locus_tag="ACG8FK_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5098061.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4738..5586)
                     /locus_tag="ACG8FK_002509"
     CDS             complement(4738..5586)
                     /locus_tag="ACG8FK_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5098062.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2064 a         1061 c          942 g         1618 t
ORIGIN      
        1 tatctaatgc tgcatattga atttgtacct cttttccaga aaaagacttt ctgccattat
       61 tagaaaagga gatagacaaa ccgtactatg ctaaaaatgc attaatcttt ttcattttcg
      121 tatacaaata agaggcacta tagtttgttt gctgactgaa actgataatc gaatttgtct
      181 gacagctgaa caaagaagtc aagatctgat aactgactga ttcgttgcag tatcttccaa
      241 atagacgata atagcagtaa gccggatcaa ttccttcaaa attcaacttt aaaaaaggtt
      301 tttgcttatc aatatgcagt tctgttagat tgagacgctc aaagtcttgt tctaattctt
      361 tgatgtatag taaaaaattt gattcagaca tatggaaatg atctgctaaa tattgtgtag
      421 taataaagaa acgatgttca gataggaaaa gaaaaatatc taattttgta ttgctttttt
      481 tcttcaagat aaacgtttta atcgcttctt gagtttgcat gaaagaggct cctttttact
      541 taaattgtta aaacttgaaa atattactgt ttttttcata aatttagaca aaaaaacagt
      601 aaacatatag gtagaatctc tactatctta tttcttgcat acaaaaaaat caaggagtga
      661 gagagttatg agatattcaa ccatcttaat atcaacattt gcagccttaa gcttatcgct
      721 tactataaat cagacagtcc acacagagga gacagaaaca gccaaatgga cagaaaccaa
      781 gtgggccaac gtcaatcgaa ttacctttca ggatgaattt tcatccttca attcaggagt
      841 cgcaaaatta agaaaaggac aaacagcgac catcaaagca aaaattgact attcgggaga
      901 cttagcacct atactacaat cagctgttac atttgagaat gttgatccca ctttgtcgat
      961 tggctatgac gagaacactt tgactatcca acacaagcaa gcgatattta ccttcaccgt
     1021 tacattaaat caggacttgt taaaaccagc gctttttact gtcaaggtat cagatggact
     1081 accgaacgat catcatttaa cgccgtatag tcagcggcag acaattgagc aagattcagc
     1141 catcgactca ggaggagatc ttgttgaaga accaacagat aaaccagaaa atgaaaataa
     1201 gccagaagta ccgcctacag aaaatccaga cggagaacag aaaccggaaa tagaacctgg
     1261 agaagaaccc gatacagaaa ctcaaccaga gccagacaat aaatcaaaac ctgaaataac
     1321 ccctggagaa aaaccagatg taactccaga accagatacg gattctggaa accaaacagt
     1381 accggaaaca aatccagaca cagataatga aactgaaaat ccagaaaaac cagaggtaga
     1441 tccagaagaa aaaccagatg tgactccaga accagatacg gacgcaagag accaaggaat
     1501 accagaaaaa ataaacaaaa aaacaattca agaggatgga aaaaaagaga gtaaaaaatc
     1561 aaatctagca attctaaaaa taaatgaaga gcagctgaat aaaaaatcac gaatatttga
     1621 tagcgcccaa tcagctgaaa ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa
     1681 acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc
     1741 ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga
     1801 tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag
     1861 aaaatgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt
     1921 tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc
     1981 actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg
     2041 ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt
     2101 ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc
     2161 gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat gaaagataga
     2221 ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc
     2281 gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgta
     2341 tttaaaagag cgacaacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta
     2401 tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt
     2461 gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata
     2521 gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca
     2581 ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca
     2641 ggatctgcat cttttggctg atgctcgtaa aaattaataa cggactggat catgtcaaat
     2701 gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc
     2761 agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt
     2821 tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata
     2881 cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt
     2941 tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata
     3001 aacacgttat tcattatatg atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc
     3061 taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta gcagtttcag
     3121 ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg ttgcagtgta
     3181 tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat accttctgct
     3241 tgtttttgca acgcagcacc ttgagcagtc agcgtaacat ttacgattct ttcatcaccc
     3301 gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa gggagtcagc
     3361 gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt tttcttttcc
     3421 cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt gaggagggga
     3481 gtgtagtgtc taatgatttc tttagaggcg gcatataaag gaaaacatac ttgattaact
     3541 aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc atagcggatt
     3601 tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt ccagctgtag
     3661 cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt tcttccagag
     3721 aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt tcactttctg
     3781 aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt tgctgtttga
     3841 tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt
     3901 tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat gtacgatcgg
     3961 gattatgcac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa tacatttttt
     4021 tcataaaatt accaccttta attatatttt atacaattga attttacaca atataattgt
     4081 ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga aacagacgcg
     4141 ctttctcgaa tagcaaacat atacaaaaaa tatcagagac aaaaaataat ctcatcataa
     4201 acattacata tatttctcaa aaagttgttt tctttcttca aaaattactt cacgatggac
     4261 ctgatgaagc atccataaat aaccaaaagg atctctgaac attgagttag tcacaccaaa
     4321 gtccatcatt tctgtcattt cttgaatctc tacacagcca tttcgtaatg ctttattatg
     4381 tgtgtcctcg atatcagaga cgatgatatt gaaccagatc gtatttggat gttcagcagt
     4441 aggagcagtc aaaccaagag acgtgttttc atccagcata tggaatctcg taccatacaa
     4501 ggtaaaaatg acttcattat tgcctctagg gtaatctgtt gcctcaatca gttgtatttc
     4561 atcaaagatt gtttgataca gttctagtgc tttcaaacta tcatttaata caaaatcgat
     4621 ttccgttcct ttatgcataa agatctcctc tccttttcag tcgaacagtt actagattct
     4681 tttattatac cagctaaaga aaacgcattc aaaaaaatag cggatttact taccaactca
     4741 ttcgattgga tgataagtaa atccgcgtcc cattacttct gctacgctgc tgacaatgac
     4801 aaaggcactt ggatcgattt cttctatcaa tggtttcatt cgggaaaatt catgatcagt
     4861 cacaacgatc aataaaacct cttttgactt tcgattatac ccgccttttg cctctagcag
     4921 ggtaaggcca cgtccaatct cagcacttaa gcgaaggcgg atgtcttcca aataatgttc
     4981 actcatgacc ataacagatt tctttcgatt gatacctgtt tcgatataag tcatgacaat
     5041 tgaagtgatt gcaatagata aaatagcata aagaaactct tctattccaa aaataaacag
     5101 attaagagaa acaacgaatg cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa
     5161 atatttttta aaaatgaggg gagggatcgt cgtaccgccg ctagaagagt tgtttttata
     5221 taaaatcgca actcctaaag caaaaatcgc acttccaaaa atgacagaca acaaccgatc
     5281 tgaagtaatc attgtttctg gaactattgc aatgattaaa ggaaaaacca agcttccgaa
     5341 aagtacttta aaaaagggtt ttttacctaa aaatagaaga gctagtacca acataacgat
     5401 attcaacact aaaataacta tagctcggtc aaagcctaaa gcagcttcca gtaatattcc
     5461 tagaccgctg acacctccag cagcaatatg atgaggccct aaaaacatat tgatagcgac
     5521 tcctaatatg aagagggcaa aaataatctt aatgtatgtc catataaatt gtctcgtttc
     5581 tttcatttat tcacttcctt aattcattaa taatactctt atcttattat aacttacttt
     5641 tcaccttaag ttaactttaa ttaataaaaa aattaggcta gtcta
//
LOCUS       DAZUMV010000146         5595 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000146 DAZUMV010000000
VERSION     DAZUMV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.149"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..863
                     /locus_tag="ACG8FK_002510"
     CDS             6..863
                     /locus_tag="ACG8FK_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5098063.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            885..1394
                     /locus_tag="ACG8FK_002511"
     CDS             885..1394
                     /locus_tag="ACG8FK_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS5098064.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1431..1832
                     /locus_tag="ACG8FK_002512"
     CDS             1431..1832
                     /locus_tag="ACG8FK_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098065.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1944..2297
                     /locus_tag="ACG8FK_002513"
     CDS             1944..2297
                     /locus_tag="ACG8FK_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098066.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2331..2774
                     /locus_tag="ACG8FK_002514"
     CDS             2331..2774
                     /locus_tag="ACG8FK_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5098067.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2791..3876
                     /gene="uxuA"
                     /locus_tag="ACG8FK_002515"
     CDS             2791..3876
                     /gene="uxuA"
                     /locus_tag="ACG8FK_002515"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5098068.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3869..4582
                     /locus_tag="ACG8FK_002516"
     CDS             3869..4582
                     /locus_tag="ACG8FK_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5098069.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(4789..4938)
                     /locus_tag="ACG8FK_002517"
     CDS             complement(4789..4938)
                     /locus_tag="ACG8FK_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098070.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            5334..5534
                     /locus_tag="ACG8FK_002518"
     CDS             5334..5534
                     /locus_tag="ACG8FK_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5098071.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1941 a          856 c         1216 g         1582 t
ORIGIN      
        1 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
       61 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
      121 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
      181 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
      241 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
      301 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
      361 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
      421 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
      481 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
      541 tcactatggg ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg
      601 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
      661 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
      721 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
      781 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
      841 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
      901 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
      961 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     1021 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     1081 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     1141 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     1201 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     1261 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     1321 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     1381 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     1441 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     1501 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     1561 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     1621 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     1681 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     1741 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     1801 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     1861 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     1921 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     1981 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     2041 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     2101 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     2161 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     2221 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     2281 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     2341 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     2401 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     2461 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     2521 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     2581 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     2641 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     2701 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     2761 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     2821 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     2881 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     2941 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     3001 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     3061 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     3121 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     3181 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     3241 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     3301 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     3361 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     3421 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     3481 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     3541 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     3601 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     3661 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     3721 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     3781 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     3841 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     3901 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     3961 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     4021 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     4081 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     4141 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     4201 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     4261 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     4321 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     4381 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     4441 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     4501 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     4561 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     4621 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     4681 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     4741 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt
     4801 ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc
     4861 tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc
     4921 tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa
     4981 cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta
     5041 agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa
     5101 tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta
     5161 tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct
     5221 attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa
     5281 tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata
     5341 acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg
     5401 gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag
     5461 aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca
     5521 ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt
     5581 ttgtacgaaa aaatt
//
LOCUS       DAZUMV010000147         5569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000147 DAZUMV010000000
VERSION     DAZUMV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.150"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..660)
                     /locus_tag="ACG8FK_002519"
     CDS             complement(205..660)
                     /locus_tag="ACG8FK_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFS5098072.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1280..1696)
                     /locus_tag="ACG8FK_002520"
     CDS             complement(1280..1696)
                     /locus_tag="ACG8FK_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098073.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1698..1979)
                     /locus_tag="ACG8FK_002521"
     CDS             complement(1698..1979)
                     /locus_tag="ACG8FK_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098074.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2355..2879)
                     /locus_tag="ACG8FK_002522"
     CDS             complement(2355..2879)
                     /locus_tag="ACG8FK_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5098075.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            complement(2896..3864)
                     /locus_tag="ACG8FK_002523"
     CDS             complement(2896..3864)
                     /locus_tag="ACG8FK_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5098076.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(5031..5186)
                     /locus_tag="ACG8FK_002524"
     CDS             complement(5031..5186)
                     /locus_tag="ACG8FK_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098077.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(5205..>5569)
                     /locus_tag="ACG8FK_002525"
     CDS             complement(5205..>5569)
                     /locus_tag="ACG8FK_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFS5098078.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1675 a          907 c          942 g         2045 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt
      841 gtgattttta attggtctag ctagttacta gcttctaatg cttgatttta cgcgatttct
      901 cttttttagt tctttgtgca tttttatctg tcatttttta aagacttgtt atttttgaga
      961 ggatttgata tgcttttttt gtctttctct agttcttcac tttgtaatat ttttatgttg
     1021 atgaatagga taatctcatt ttgggctgct cgtgagagca gtcttttttt gtttcagggt
     1081 acgtcctatt ctttggattt ccgcgctatt aaataaccga tacttgggga ttggggtagc
     1141 gccccaaaat acaaaaagaa gaagttgcat aagcggattt ttcttttttt tcgcaagttg
     1201 gtaccaactt gcaaatcttg ctggaagact gatccctaaa aagaaatcga ggaatttttt
     1261 tagggatcgg tttctttttt tatcttttta gaaagtttgt tgggtcaaaa tttccagaag
     1321 taaaggtttg attggtttct ttttgacgat ttttgggttg atttcgtcgg gaaggattga
     1381 gttgattttc aattttttct ttcacttctt gatacacttt agaatttgta ccgataagaa
     1441 caccagtatc ttttagatat aaatcagcaa attctgccat gatttctgtc atgatagaaa
     1501 tttcttttcc catggcattg agttttgctt ggttactggt tgtggttgta ttggtatcag
     1561 gttcataact catacataaa aaacgaatgg cttctgaata attgttgacg tctggatttt
     1621 tttcgatgat ggaatctaaa acgtcaacgt gactgggata aagggttacg tgtttttgaa
     1681 cagaagtttt tctcatgtta tattcctccg ataagtgttt caatgagttg gttgtttgtg
     1741 tgaacatagt cattcagtat gttcgtattt tctgtttgaa tggcaataaa tttttcgagt
     1801 aataaatcga attgatcttt tttgtaattc ataagttgac ggtcaaaatc actttcgatt
     1861 agaactttta gatatttatt tcgactccat tttttgcccg ttttggttgt gagttcctga
     1921 caacgtttat ctattcttgc aataattttg ggattgatat ttcgaactaa aatgtccatg
     1981 gaaattttcc tttcgtatca gcgtataatt tatgtatacc atacaaatat agtatttgta
     2041 tagctatttt tcgccaaaac actatacata gcctagccac aatggcaaaa aagtgatagc
     2101 aaattttttt ctgagaaacg tgataggcac agcctatcta gcacctttcg taagaaagtg
     2161 ataccacgtt tccttatgct ctttcaaaat tcagaaaaat cagctgtaaa acgagtgcag
     2221 tccgagtaag attcaacgaa aaaacgggga ttggggcgcc accaaaatat cgcaacatgg
     2281 gtccatgttg ctctgctcgc aaaagcaaaa agaagaataa aaccagcaat ttttattaat
     2341 ttaaattgct ggttttattg ccagaattgc caccattttt tctccgcctt tgattgtttt
     2401 tctatcgatt ctgtttcttc agaaagagtc gttgatttat tttccgaaga ttcttccgta
     2461 taagtcaact gtagttgttc ctgcagtttc tcgatttgtt ggttggcttg cagggtgagt
     2521 tgttgttggt ggtcgagtaa tttttgcatt tgttttattt gctcgtcttt ttcttgtagt
     2581 tgcatatctt tttgatttaa ctgttgttct aggagagaaa ggacttctaa ttcgggaact
     2641 tggttggcgt ttttgtggtt ttcggttttc gttttttcgc caactagttg ttgatttgag
     2701 ttttcgtttt cgttaaaaaa cattgatttt atagcctttt gtccggtttc attaatcaca
     2761 aacttgtttc cgattttcga aaaccaaagt tttcgatttt cgtcagaaac ttttttgtta
     2821 attgctgttt tactaactcc taattcttca gcaagttctt tgattgtttt ttctgtcata
     2881 aaatctaaaa acccctcaat ctttgaataa gttttgaaga ttgtctaata agtctttttt
     2941 tagttctgaa ggaatatcgg aatttatctc tgatttttgt gcattatttt ctttattttg
     3001 ggtattttgt tctctttcta acttttctaa ctgttgtaaa agttccaatt ttcgcttttg
     3061 ttctttgatt ttttcttgta atgaagtagg tgctttttca gattgaactt gttggttagc
     3121 tagttctaat gctttgtttt gttcttcttt tgttggggca tgtttgttaa agtcgtctga
     3181 tttttttatc tcaggtttaa atgtaaattg atagccaatg acaggctttc cccgtccttt
     3241 tccgtatttc tttttgattg ttaaaccttt aaagattgcg gttagttctt gtcgtattgg
     3301 attgaacaca cgttgatcaa tcgtaccttg gttgtaactt ttaggaatat caagtaattc
     3361 atgaaagtct tcttttgaaa aataagcgaa acctttggtt ctgtattgtt ttagtaatcg
     3421 aaacattgtt tttgaataac tgctttctaa ttcattgaat tgttggagag aatagcgcac
     3481 ccattcttca agattattaa gtaagggtaa ggctttttca tgaacttgta tttcagcaaa
     3541 aggaatatca gattttccgt cgattttaaa ttgattaaat aaaacaaaac gttcacgttg
     3601 caaaccgtca gcacttcttc taccaaaacg taaatccatt aatttgtcat acgttgtttc
     3661 taaatcatct atgaatcgaa catttgcagt tgctttataa ttgcttaaat cttttaattg
     3721 ctcgaatgta aattgtactt ttttatcacc tacatcacgc attcttgata cgatagaaaa
     3781 gaacaaattc atttcgactg gtgtaaattt tcgcaacgga attgtattta attcatgatg
     3841 atactttacg atttcattag acatatttaa aatagctcct ttcttactcg cacaagagat
     3901 acacgttatg aaatacaaaa tgcctgttta tctacatctt atattaacat cgtcgatgta
     3961 ctaattcaat gtacaaacag acaaaacgat gtagataaac agacaaaacg atgtagataa
     4021 acagacaaaa cgatgtagat aaacagacaa aacgatgtag ataaaagcgt cattcccttg
     4081 ggagagtaag gatatagccg acggtaaaga ggtttaaaaa gaggtttaaa aagagattat
     4141 aaaagagggg gacaaaattt cccgcctaaa aaaccacttc ttttttctcc aaacagattc
     4201 gcgcattttt ctcaactggg aatcttactc cctacagtcg cttaaaaagg tgtgatcgct
     4261 ctgctctcac attatactgc gcagtcgctt ccggctcctt tgctaaaaat gttcataagg
     4321 aactctttga gaagctatga gccttcgttt gagacgtttt aagacagtct tagtataaat
     4381 cccttatctt gttcttaaag cgccttacag gcggtcaggg caagccctaa caaccctaaa
     4441 gttgtctgaa tgccctctaa aattcgattt aagcactctt aaacctactc ttatatattt
     4501 atatacttag acgtttcaaa cgtcttaaaa cgcaaattag agcttgataa agatatttgc
     4561 tcgtgtgccg acttttttaa aagtctaggc acacgccatg acaaagctga aataaactgc
     4621 ttcagctttg tcatggcgga ttcgctcact tgaaaaagtg ttcatttcgc tcgattatta
     4681 gcatatattt tttaactttt tttgctagcc aaattttgca ttagttcgct cataaatccc
     4741 accctttcag tacgtcctac ggacgtatcg ttcgtaatgt gaaagtacct ttaaaggtac
     4801 tttctacaaa actcactaaa gtgtttggca agctctatat ttgctagcca aatacctacg
     4861 tatttggtgc aaataaccgc ttgtcaaagg ggcgcaagct cccccttgaa tcccccctca
     4921 aaacccacgc tccacaatac aaaaaaggct agcaaatgct agccactttt tgtaaagttt
     4981 cgcttgtttc accttcggtg aaattgttgc tttgcaacga tcaggtagag ctacattttt
     5041 tttcgttgtt caaatattct tacgccattt actattaaaa tcgtaggtat tccaaagtaa
     5101 agaaaaaatt gattagcagg taatacaaaa tataaaataa taccagcaat aattaccaaa
     5161 cttatagtaa gttttgttaa tttcatgaag tatgccacct tatcttatat agatatcaaa
     5221 tagattaata tgccgattgt attataaaat ccatgagcaa ctatggaata ccatatattt
     5281 tttgtttgta tataaacaaa acttaaaata ataccaataa agaaaatact taggcctccc
     5341 ccatgaacaa tagaaaacag aaaagaagaa attagtattg aaatatattg ttgttttttg
     5401 tttgcttgag gatcaatcat acaaaatcga aaaattcctt cttctaaaaa agctccataa
     5461 gtagcagcat ttaaaaagaa taaaaggaga gaaccattta cttcattagg atttatacct
     5521 tctgaaaaag atgtatttag aatattgatt aggaataaaa cagagaatg
//
LOCUS       DAZUMV010000148         5563 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000148 DAZUMV010000000
VERSION     DAZUMV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.151"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..150)
                     /locus_tag="ACG8FK_002526"
     CDS             complement(<1..150)
                     /locus_tag="ACG8FK_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5098079.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRW"
     gene            complement(243..638)
                     /locus_tag="ACG8FK_002527"
     CDS             complement(243..638)
                     /locus_tag="ACG8FK_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098080.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(628..840)
                     /locus_tag="ACG8FK_002528"
     CDS             complement(628..840)
                     /locus_tag="ACG8FK_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5098081.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1912..2514
                     /locus_tag="ACG8FK_002529"
     CDS             1912..2514
                     /locus_tag="ACG8FK_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFS5098082.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2869..3105
                     /locus_tag="ACG8FK_002530"
     CDS             2869..3105
                     /locus_tag="ACG8FK_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFS5098083.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            3102..3515
                     /locus_tag="ACG8FK_002531"
     CDS             3102..3515
                     /locus_tag="ACG8FK_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5098084.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            4058..4984
                     /locus_tag="ACG8FK_002532"
     CDS             4058..4984
                     /locus_tag="ACG8FK_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFS5098085.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4981..>5563)
                     /locus_tag="ACG8FK_002533"
     CDS             complement(4981..>5563)
                     /locus_tag="ACG8FK_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5098086.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1980 a          806 c          931 g         1846 t
ORIGIN      
        1 ccaacgataa atcgtagtat gacaaacatt tattccacga tcatataaca attcctgaac
       61 ttcacgatag cttagattgt aacgcaggta gtaaccaaca gcgacaataa tgacgtcttt
      121 tttgaattgt ttgcctttaa aatgattcat tactctgtcc tctctgtctt ttttctcaat
      181 tttacactaa aatagatttt ttggaaaact ttgcaacaga acctttgtat gaacctttat
      241 ttctacattt ttttctcaag cacactactg ataatgatta aaataatcgg ctggattgca
      301 atgatacttc cgacaataat agctggaatg atgtagtcta aacaaataaa tataactgtt
      361 gccaccccta acagtattgt gttaaacata tttgttatat ttcccgcttt ttctttgatt
      421 atgatattcc gttcgtccat aacattgatt ttgtagtctg catcattttt aatcatttta
      481 tcatgcaaaa tttgaacatg agaaacgcca aaaacggcac tgaataaaat agcaagtcca
      541 atatctacca ttttaggaaa atctgtaacc aaaattgtca caatcaaaaa caacgaaaca
      601 atataaccga tataccaaat ctgaccctta tttttcatct tcactttcct cctcatagat
      661 aaaaatttct tcaatgctca tttgaaagta ccttgcaatt ttaaatgcca atataataga
      721 gggattataa cgtccatttt ctaaagaacc tattgtttga cgagatactt caagagcaga
      781 agctaaatct tcttgtttaa ttcctctttg ttttcgtaat tcttccaaac gatttttcac
      841 aacatgctcc tttctaaaaa atggaaagtt tactttcctt tcgtgctttt ataatactcc
      901 tgcgagcaca aaatgtcaag gaagctttcc atataaattt tgattttaat gttttagtta
      961 ctgtttttat gacacatgaa cacacttatt cttatttcct cttatctgct atcaacagaa
     1021 aagaaaaagc cctgtcaaga gccgattaga tcaagccagt cgagaaacga tgttagacca
     1081 aattgatttt gggtaatgaa tgtttagaac ttagtagtca tccaaaaacg actacttttt
     1141 ttgttgaatc gtccaaggac gattcgatgc ttttgacttg taagaacagg attgaatgaa
     1201 accatactgt tcgcatattt ttagtggatg aacaaacaaa atacgagaga ttttttgttc
     1261 gttcatccat ggttttagaa aaaagaggga cgattttgga agaagaaaat cgtctctttt
     1321 ttttcttctt tttgtatgac aaaaagaaag atcttttgcc cattttattt ttataaaatg
     1381 ggtaggtggc gttcgcgtaa agcaaatcga tacaatccaa tggttttggc gaatcagatt
     1441 taccattcta ggcttgttta atacagacat cgattgttat tttgagaatt tatatcctta
     1501 ttggcaatga aatggatatt ctccatacca atagaaatct tcaagataat tttcggaaat
     1561 atataagagt taatcaatta tgatcatata gtaggtaaaa attaggacag ccttcaatta
     1621 tatgggaaac caatataatc aagtaattat tcgggtaaca caaatactag agtactcatt
     1681 tcttataact gttttacacc tattagggat cagtattatt aatagatcta gataaattaa
     1741 tacgtaatta tataataatt tatgtttgtg ccattaaaca atcttaacag atatttcatt
     1801 ttatttatgt aaaacaacac gaataattat tgcttcgtaa agttatatga gtataagtta
     1861 atttactgga ggtagtgcaa tgcttttttc tgaaagattt ggttttaaaa aatggaggat
     1921 atcatactga gagatagatt gacagaccct cttaaaaata gtttagcaaa tgtgatttat
     1981 gatatattac gagaggctga taaagtttat agtaattata atggaaaagt ctcatgggta
     2041 atatattctt ttttaattaa agagtttttt aaagaacaga taaataatag ctatgagttg
     2101 ggttatcatt ttactgttta ttatgaagat attatcaaga ggttagaaga cttggagtgg
     2161 tatgcttatt atgatttatt agaggttttt gcaagtgcta aaatcttgga taaaaagaaa
     2221 agagaggaag taaataaaat tctggagtcg gaaaattctg gttacagatt aagcgaaagc
     2281 aatgaattta ttccaatcac agatgatatt tctattaagt ctataaatgc tgtatctgaa
     2341 tcaccattta attacgcaaa aaatcatatc cataaatctc ttacatatat aagcgagaag
     2401 gaaaatcaag attataatac tgtagtgaga gaagcaatta gtgctgtcga aagttgtctt
     2461 atcgaatttt caggattacc agctaataaa aaaatacttt gggaactgct ataaaagcaa
     2521 ttaaagacaa atcgaataac aatgtcgatc ttacattttt aaaaccattt gagacaatgt
     2581 atggtttggc gagtaataat ggaatacgac atgcagaaaa tgaaaaaaca attgtctcag
     2641 atttacctga tgcagttttg gtgttaacaa catgttcagc cgtaattaat tatttgggag
     2701 taaaaatagc atatgactcc aataaatcat gaaaagttaa aattcatcca agagctacat
     2761 tataactatt aatttccaac tctttaaaag tttagtaatt gaaggaagta ataagggtga
     2821 ttgaacaata cggatatacg gtgtatactt atcgtaggag ttgatgatat gaatattaaa
     2881 aaacgcaaaa tcagagaagt aggtaattca atagttgtaa ctttgtcaaa agagagttta
     2941 ttgcaaaaag gtttgaaacc tggagatact atttttattg atgaagataa aatgatggaa
     3001 gctattgtaa aagaggaaag caagatagat cttgaaattg atatgtatgt taaccaagct
     3061 ttatctgagt atgatgcagc tttcaaggaa ttagttgaaa aatgatctat ctaagtgaaa
     3121 aagatattgt aaaattgaat gttattcaaa taaagaaata ttcaccgcga gaaacaatcg
     3181 gagtcattga caaaaatgcg ctgagtatgg ctgtaaatca acctaaacag catatttttg
     3241 gaaaagatct gtatcctgat aaatttagca aagcagctgt tctgctaata aatctgatca
     3301 aaaagcatcc atttcataat gctaacaaac gtacagcatt tttagccgcg tatgtttttc
     3361 tcaagctaaa tggctattca ctaaaaatga aaaatcagga agtagtagaa tttgtagttg
     3421 aaattgctac gtatcagggt gattttgatg acttaaaaga ggcaacaaca gatatactaa
     3481 aaaagaattc aacaaagacc gattcaaaaa agtagaagca aactagggat tcagttaaat
     3541 ctgaagattt agtttgttct ttattttagt ttacataaga tacattatgc gaagttcatt
     3601 attttaaaag cttaaataat aaactttttt ataataccaa tttatttatg taaaccaatg
     3661 accttaatcg cttaagcaag catgttatgt aatactctac tctctccaaa actaatgact
     3721 atctattttc aaagaagcta gaatgattat ctgatcaaat gataatttat atttaattcc
     3781 tggtctactg ctcctctttt aatcaattaa tgtctaacgt actgtacacg ataaagtagg
     3841 aagacttatt ttcaacttcg gattcatttt tttaattcgg tgaaaatcac taaattaaga
     3901 gtttatccaa gttcatttta gctgagaata cgcttgaaaa taattttgat gaattttcct
     3961 gaattaaaat ttattaactt gttgagtgat ataattgtaa tgtattacca aatagttagc
     4021 acttagatta gtgatggaaa ggaatagtga gaacaagatg actggtagaa atcccaaagt
     4081 atttatatca tattcatggg agaataagga aaataaagaa tgggctagag aactagcaga
     4141 caaattactt gaaaacggta ttgatgcata tatagatcag tatgatttaa gtttaggtga
     4201 tagattacct cagtttatgg agcagcaaat atccgattca gattatgttc taattatatg
     4261 tacaccagag tataaaaaaa aggcaaataa tcgtacaagt ggtgttgggt atgaaggtca
     4321 tattatctca gcagaattgc tttctggaaa aaatgaacgt aaatttatcc caataattaa
     4381 agaaggacgt gcaagcgaag tacttcctac ttttcttgct ggaaagcttg gaattgattt
     4441 atcagatagc cagcattatg atgaaaactt agatgattta attacaacta tatttggtgt
     4501 taagagaaaa ccagcattag gtgatatacc taggcatatt aaaggcagtc ctttactacc
     4561 tatagaacat aatgaaaata aagatattga aatcttagga attatcactg ataaagtaac
     4621 tgtacctaaa atggatggct caaggggttc tgcattatat aaaattccat ttagattaaa
     4681 tagagtacca agtcagcttt ggaaagagtt gtttgttaga aattggaatt caccaccacg
     4741 atatacaaca atgcatagac caggaattgc ttctgttgta gacgatgaaa ttttacttaa
     4801 tggaacaact attgaggaag tccgggaata tcatcgggaa actttaaaac tttgcattga
     4861 aaaaagtaat actcaagaaa aaaaaatatt gctagaggag gaacaaaaaa aagagagaga
     4921 gaatgagcga gtcagatcac ataaagaaag tgttcagaaa ttagcagatg aattagagtt
     4981 ctagttgata gtcagttctt tcttaattcg atacacagtt tgtctagtta cattatagtc
     5041 tgttgctatt tttgaaatag caacaccatt ctcctggtcc tgaacgatgc ttctataaac
     5101 aaggcgacgt tgaggatctt ttgtgttcgc actgtataac ttaggacgtc ctttatagac
     5161 gccgtttgct ttagcaatct ttatcccttg tgcctggcgt cgttttgatt cagtacgctc
     5221 ttgttcagca atcatggcca agatttgaat gattaggtct ttgataaatt tatccaggag
     5281 aggatttccg attgcttcag ccatgatcgg taaattagtg ataataagtc gcacattttt
     5341 cttctttaga taatttactg aatcgataat ctcgtcataa ttacgtccaa gtcgatcgat
     5401 agcctctaca ataaaaatat cttggtctct aacaaaatct agaacttcct gaaaaatcgg
     5461 tcgatgagta atagtcgctc cagattgttt ctcgatgaaa attttttcag caccaaattt
     5521 tctcatttcc tcgatttgac gttgttcatt ttgatcgatc gat
//
LOCUS       DAZUMV010000149         5323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000149 DAZUMV010000000
VERSION     DAZUMV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.153"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1258
                     /locus_tag="ACG8FK_002534"
     CDS             <1..1258
                     /locus_tag="ACG8FK_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS5098087.1"
                     /translation="QEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIII
                     SILLFSLFLIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQ
                     TGTIENELTDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKL
                     TEQLTDSNSEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILA
                     FALGGEIKLTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPIS
                     MNQYLKEKFRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFA
                     RDYRLLLLNWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNI
                     PVIGLVLILSALWIVYYQRTFWKKFE"
     gene            complement(1509..1988)
                     /locus_tag="ACG8FK_002535"
     CDS             complement(1509..1988)
                     /locus_tag="ACG8FK_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5098088.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(2004..2756)
                     /locus_tag="ACG8FK_002536"
     CDS             complement(2004..2756)
                     /locus_tag="ACG8FK_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5098089.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            2873..2943
                     /locus_tag="ACG8FK_002537"
     tRNA            2873..2943
                     /locus_tag="ACG8FK_002537"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2905..2907,aa:Gly,seq:ccc)
     gene            3304..4029
                     /locus_tag="ACG8FK_002538"
     CDS             3304..4029
                     /locus_tag="ACG8FK_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS5098090.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            4284..4550
                     /locus_tag="ACG8FK_002539"
     CDS             4284..4550
                     /locus_tag="ACG8FK_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS5098091.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            4547..4978
                     /gene="ruvX"
                     /locus_tag="ACG8FK_002540"
     CDS             4547..4978
                     /gene="ruvX"
                     /locus_tag="ACG8FK_002540"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS5098092.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            5003..5308
                     /locus_tag="ACG8FK_002541"
     CDS             5003..5308
                     /locus_tag="ACG8FK_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS5098093.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
BASE COUNT         1720 a          950 c         1053 g         1600 t
ORIGIN      
        1 acaagaacag gttttttcag caaagatttt ggtagttgct ttgaccatca ttccttttgt
       61 ttttccgctt ctagtcatgt ttattttaac cggatggcga gctgatctag ggattttgct
      121 tactatcatt atctcaatcc ttttgttttc tctatttttg attattcttt tttgtctggc
      181 tagtttgatt gtttttggtt tggctcgaac agcagtgttt aaaaaacata gaaaaacagt
      241 ttctagtatc ttgcttggca gctcgatgct gatcgcattt gctggaatca tgtggatgaa
      301 tcatcaaacg gggactattg aaaatgagtt aacagatcgg catccgattt cgatattatt
      361 gccattctat gatgctgcca gtcagccact ttctcaacaa ggtttattgg gttggggaat
      421 actagctatc acggctctgg gatttttcct tgctgtaaaa cagctgatag taccaaagct
      481 tacagagcaa ctgactgatt caaacagtga gttcaaaata aaaagaaccc ataaaaagaa
      541 ccaaaatgtg catcagcttt tatttagtta caatgctcaa ttagtaaaag aacctaattt
      601 gattatgcaa gttctttctt cttccttgtt aactccaatc atttttattc ttgcttttgc
      661 cctaggaggc gaaataaaat taactgattt agataataag ttaatcggtg ttgttttcct
      721 ggtagggatc gcattggctt tcttaacagt taatcagact tcttttatca gcaatcttat
      781 ttctttggat caagaaaatt ttctgtttat ccgttctttg ccaatctcta tgaaccaata
      841 tttaaaagaa aagttcagat tcggatggat attgcagagt tttttaactg gtgggatcgc
      901 attattaagc ggactgtctt ttcagctgcc tttgtttttt atccctagct tgctatttgg
      961 ttctttattc ggctgctact tattgtcact acgttatttc gctagagatt atcggttgct
     1021 gctgctgaac tggaccaata ttaaccaatt atttaacaga ggttcaggaa gtttgggttt
     1081 agtcgcaact atgatgggtt cattgatcat cagtattatt cttatcgtct tatacggaat
     1141 gttggctttg ttttatccat tttggttagt gaatattcct gtgatcggtt tagttctaat
     1201 actttccgct ctatggatcg tttattatca acgaacgttt tggaaaaaat ttgaataaag
     1261 aagaggggcc gtgacaaaaa tcatgtcgca gccccttttt tacagataga gggtgttcaa
     1321 aagtcagttt ctcagtatta agtcaatttt atgtaaaaat gtgagaagct atttttacaa
     1381 aacttttttt aatactcata attacttctt attgctcctg cggttacttg tcgcaatgtt
     1441 tgtgagatat tttgtcatcg ctcaaggctg aacgtctctt tcacaacctt ttctttgtta
     1501 cacttcgttt atttaaaaag ccgttttttt cgtttcatca tgattgcgtc acgctttact
     1561 ttgaaattac ggatgttatg gttaaagact tctgataata caagacctaa agcgattgct
     1621 ccagcaatca taattgcttg gaccgcataa tgagccgctt ctcgatagga ttcagtaacg
     1681 agatttcgaa ccgcttgata agctaaacca ccagggacta gaggaaccat cccgggaata
     1741 ttgaaaatcg ttacgggcat tttcaaacgt ttagagaaaa gatcgctgat gaatgctact
     1801 cctaaagaac caagcaatga accaaaagca gcagatccgc ctatttgaac acagatccag
     1861 tagatcatcc agccaaaagc accagaaaga ccacaacaaa tcaatgaacg cctaggtaca
     1921 tttgtaataa cggcaaatgc cgcagtcgct aaaaaactaa aagaaaactg aataaatatc
     1981 gtccccatac ttgtgttcct ttcctaataa aaaagctgga aaataaaagc aatcgcaaaa
     2041 ccgatcatcg tagcagtcat catcgcttct gttccgcgag aaactcctga gacataatgt
     2101 ccagctagca aatcacggat agcgttcgta atttgaactc caggaactaa aggcatcaca
     2161 catccgataa tgattagatc ctgattgctt cctaagccaa aacgtacgct gtagatagcg
     2221 gctatgccaa tcaataaaga agataaaaat tccgataaaa atttgattct aaaaaatttc
     2281 aaactatata gataagctgc gtatcccaat cctccaatga ctaaagtcac aggtaaatcg
     2341 gtcatctttc cgccaaataa caccataatt gttccactga tcactgccgc gctaacgatt
     2401 tgcaaccaga caggaaagaa agaacggtct cggtcgattg cttgcagttc gcagtaaagt
     2461 tcttctaatg ttagctctga agcaacatat ttacgtgaaa gatcatttac ttttactact
     2521 ttttctaaat taatcgaacg gttggtaatt tgttccattc ggatggtcga ggtacgatca
     2581 agaccaataa agagaccggt ttgagtgaca tagctgacca aacggaaatt tccagaagct
     2641 aaagcaattc gactcatcgt atcttctacc cgatacatct ccgcattgct ttccatcatg
     2701 attttcccag cgagtaaaca agtgtccagc aacagatcta attcttttcc cgtcatagca
     2761 tcttctccaa taattgattg attagtttta ttttactaca aaacatacca gaagcgtaaa
     2821 gaataaaaaa acttgaggaa ggaataaaag aggcgtatac tgtttttgta ttgcgggtgt
     2881 agtttagtgg taaaatccca gcttcccaag ctgatgtcgc gggttcgatt cccgtcaccc
     2941 gcttaaaata ggttgtgaaa aagctgctct gcaacaagta ataagaacag ccaagcaaaa
     3001 acaacttctt atgtttttaa cttggatgga cttattaccg cagatgcaag cttttgcgca
     3061 ccgtttacca agcggttgtg aaatcagtgt attgtcttga gtatgaagaa aatttttgaa
     3121 taataataaa taccttacag atagagtggt gactgattag agacgaggaa tccgtctctt
     3181 ttttgtattt ttaagccatc ttaaaacagg ttttattttt tgctgtttct atttctttct
     3241 agtatttatt tataggggaa catatgttaa gatttaggga gctgttagta aaggagtaaa
     3301 gaaatgagca aaaaaacaac caaggcgatc gtggggatcg tcgctgctgt gattgcaggg
     3361 gcattcggtg tttccaccac acaaaaagat tcagatatct ttcagcagat tacaactatt
     3421 tttgattcca caccacaagc ttctcaagta gcggatgtgc cagaatatga tggaaaacat
     3481 caagaaatag aaatcaatgg gaatcagccg gaatttacaa aagaggatct tagcctttcc
     3541 aaaggaacat gggaaagcta ttcaaatatt gataaattga acagagtagg acaagccaat
     3601 gctatgcttg gaaaagattt gttccctact gaaaagagag agcctttgta tattgacccg
     3661 acaggttgga aacaaaagaa attaagtgac gggcaatggt tatataatcg cagccattta
     3721 attggtttcc agctgactgg tcaaaataac aacattaaaa acttgatgac agggacacgt
     3781 tcgttgaatg ctccatacat gcttgctcac gaaaatgata ttgcggcata tatcaaagaa
     3841 acaaatcatc atgtacgcta ccgtgtcact cctcattttg aggggaatga actggtagcc
     3901 aggggagtgc aattggaagc agaaagcatt gaagacaaaa aaattgaatt caatgtcttt
     3961 atctacaatg ttcaagatgg ctacacgatc aattatgaga caggacaagc aacaaaaaca
     4021 aaaaattagt agcagctatt cgaatttttt atgaatatta tgaaaaaatt gtgtaaaaac
     4081 atacattcaa agaaatatag cgtatacttt atgaaaagag tacttttgat aaagctacag
     4141 aaaaaaggaa aaaaacatac aaatagtgtt tcttaattta ttcggaagtt tggaatattt
     4201 gtttgtagtt ttccctttct tcgagtaaaa taaggttgta gaaggtaagc tggaattacc
     4261 ttgcgggaaa gaggtgtaga atcatgggtt ttacagatga aaccgtacgt tttggttttg
     4321 acgatagtcg caaaaaagaa gtgagcgaaa cattagcgat tgtttatcgg gctttggaag
     4381 aaaaaggcta caatccaatc aatcagattg tcggctactt gctttctgga gacccggcct
     4441 acattcctcg ttatcgtgat gctcgaaatt tgattcgccg tcacgaacgc gatgagatca
     4501 tggaagtaat tgtcaaggac tacctctcaa atcatggagt gaatttatga gagtaatggg
     4561 cttggatgtc ggttctaaga cagtaggtgt tgctatcagt gacccctttg gctggacagc
     4621 tcaaggagta gagatcattc ggatcaatga agcagaagaa gagtttggct ttgatcggtt
     4681 gaaggaatta gtagcacaat atgaagtaga ccggtttgta gttggattac ctaaaaatat
     4741 gaacaattcc atcggaccaa gggcagaagc ttccatgtca tacggagaaa agatcaaaga
     4801 actattctcc ttgccagttg attatcaaga tgaacgatta accactgttc aggcagaacg
     4861 tatgctagta gagcaggcaa atacttcaag agccaaacga aaaaaagtca tcgataaatt
     4921 agcagctgtg atgatcttac aaaattattt agacggctca ggtaagcaat tattttaata
     4981 ataaaagaaa agaggtaatc ttatgacaga acataaccat tcccacgacc atgaaggaca
     5041 cgaacacatc acattggtgg atgaccaagg aaatgaaaca ttatatgaaa tcctactaac
     5101 agttgacgga caagaagaat ttggacgtaa ctatgttctt ttatatcctg ctggtgtttc
     5161 agaagatgaa gatgtagaat tacaagctta tgcatacatc gaaaacgaag atggaacaga
     5221 aggcgagtta gaacaaatcg aaacagacgc agaatgggac atgattgaag aagtattcaa
     5281 tacattcatg gcggaagaag aagaatagac tataaagcta gga
//
LOCUS       DAZUMV010000150         5322 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000150 DAZUMV010000000
VERSION     DAZUMV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.154"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..2329
                     /locus_tag="ACG8FK_002542"
     CDS             2..2329
                     /locus_tag="ACG8FK_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFS5098094.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2326..3240
                     /locus_tag="ACG8FK_002543"
                     /pseudo
     CDS             2326..3240
                     /locus_tag="ACG8FK_002543"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3313..3828
                     /locus_tag="ACG8FK_002544"
     CDS             3313..3828
                     /locus_tag="ACG8FK_002544"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFS5098095.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3965..5254
                     /locus_tag="ACG8FK_002545"
     CDS             3965..5254
                     /locus_tag="ACG8FK_002545"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5098096.1"
                     /translation="MKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKD
                     TAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLPF
                     YNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQFVT
                     GFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQKET
                     TFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHFIKQ
                     DVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKLISD
                     SEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQPKG
                     ILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
BASE COUNT         1889 a         1027 c         1051 g         1355 t
ORIGIN      
        1 gatgaaaatg attaagaaaa ctcaaaataa aatccaacgt atatgtcaaa aacattttgt
       61 ttctagactc aaagctgtat ctaaaagaaa atacttacat cccattatga tttcgatcat
      121 cttgattttt acattcttag ggagcttatc aagtatttat gtaaacgttt atacagaaac
      181 gtcaaatttt gctaaccaaa cgacagagga gacaaatagt ccaccctatt ctgaaataca
      241 gtccatagaa agcaccaatc agtctagcaa caacgagaca acctcaacca catcctcgac
      301 tcaactgatt gatccgacag aaaaagaaat cacagcagca aaagcagcag gggagaaagc
      361 ttattcagaa acaggtagaa gccagacatt tagtactgtt gcggccactg cgaatttcaa
      421 cggacatacc ataccagatg gatatacagt gatatatgcc aaagatggag tcattgttgc
      481 ggttaacgga tcgatgagtg cggataaaac tgcgttacaa ggcgtaacca aagcaatcgc
      541 ttttattgat aaccgtccag atatccataa tctgtttatt ggctccacag taaatggtac
      601 tactatcaca ttcccaaaaa gtatctatat gggggtggct accgctggaa atacgatcga
      661 ttcagttaca cccaatgcgg ataaaagtta ttcttttaca ccctcaacat ggggagggct
      721 tcctgccgga ggaggtatga gtactttttc aatcggtata cagttagcaa atgatattcc
      781 attcacgggg tactcgatca aaaatgggca gatcgttgat ggtaagggaa atgtttcaaa
      841 ttttccaacc tatcaagcag caacggttca ggtcaatgcg aatcggacag gaactttttc
      901 ttatacagtc aatggtggag cagcaaaaat cggaacgtac gggactacat tatctggaat
      961 ccatgtgggt gacaaagtgg ttatcactcc aacagcagta acgggctatt cttttgcaca
     1021 aacaaacggt tctttcacag ccctagaaag taacgtcgat acggtgacct atacaggcaa
     1081 acaaacacca atcactatta agtatcaaga ctattttgga cagatgattg ctccttctaa
     1141 aataatgaat cttacttacg gaagtgctgc acaagacctg acgactaata cgcctactat
     1201 caacggatat acctttacag ctgtttcagc gacagaaaca aagaaccaat cagcaatgag
     1261 tgttttagct agtttagata caaatggaaa tgtaatcgta aaggacgcaa atggaaaaca
     1321 gatttctgaa gtcatcctat attataagac aaatgcaaca gtatcaatca atgcaaatgg
     1381 cagtaagtac tatgatggtc tttctgttgt accgatcgtt aaatatgcct ttaatgataa
     1441 aacagaatca acatcccttt taaacaatga tctcagtatt gatcaaatca catggaatcc
     1501 aactgatttc aaagcgatga atgaaaaggg agttgaagta actgcgccta ctgaaattgg
     1561 aacctatacg tcttggcaat tgacaaaaag cggactagct aaactagctg ccagaacaaa
     1621 ctatttattt actatcgtgc agacatctga tgtctatacg attaagcaaa tcagcggtgc
     1681 ggtagagctc agtggcagta agacatatga tggaaaagca ggtgtcccgt caatccatgt
     1741 caagctagca gagggtgtca ctagttcgct tactccagta caagtggcac ttagcagtat
     1801 ggattacaca gcagatgcac aatcagcaaa aaatatggtt aatgctggca gctatacaat
     1861 taaactaacg aatagcggaa tagacaaagt caaagcagca aatcccaagt tgtcatttac
     1921 tgatatagcc aacacatcag gaacttatac catctataaa gctaacgcag tgattactgt
     1981 cgatgacgct gcctttaatt atgatgccca aagtcactcg atttcggttg gaaatctcca
     2041 cgttaccggt gttgtcttag gcgaatcatt ggattatacc ctaacagtta acagccggac
     2101 agatattgga tcacaaatag taacgattag tttaggcgaa ggaagtgtaa acaataatta
     2161 tacgattatc gcaacagata ccgctaagtt gacgatcaat ccgactctct tgcttccttc
     2221 gacaggtggg ataggtctac tgccctttgt ctttttagga ctgatcttta ttttttctgg
     2281 cttcttttat tttatccatc gtaagaaaaa agcaggtgaa cagcgatgaa aaaaacaaaa
     2341 aatgtacgtg tggatatatt tgctgttctc ttttttatcc tactggtctt ttttataggt
     2401 tctccgactg tgtcagctag agaatcacct attacattta ctttgcataa tcgaatgttc
     2461 gaaagcgata agatgccaag tatcagtcaa aacgacggaa taaatatccc ggataacttt
     2521 caaggagtaa aagggttgaa cggcgttact ttcgctatat atgatgtctc ggatgaattt
     2581 tacaagctgc ggtcagaagg aagtagtgtg gaagacgcac aaagaaaact agcacaaaaa
     2641 agtgatagtg aaaaaatact ggcagaaaat gtgacaaaaa cagtagacga ggaagagggc
     2701 attgcttctt ttagtgcatc ggacaaagat gagcaaggtc gtgacgctgt ttaccgattt
     2761 gcggaaataa agacttcaga ttaagtaaaa gaaaaatcag caccatttgt cgttgtgctt
     2821 cctgtgacag attcatctaa tcataaattg acgaccatcc acttgtatcc aaagagcgaa
     2881 caaaaaatac ctgctgcact cacattaaca aaaacagtag agaataagca gacagatttt
     2941 gcagatgggg ataaagttcc ttatctcatc accgtaacaa ttcctgagaa catcaacgag
     3001 ataggaacct atacgatcaa agatacagct gatcctcagt tatggctaga accagaaacg
     3061 atcgattttt tgattggtgg agacaagatt catacatttc acacacaaaa aacaaaacat
     3121 ggttttgtct tatcagattc tggaaaagat cttagttcgt ttgctgggaa aaaattaacg
     3181 atccgttacc aaatgacatt gaaagaaaac agtgaaaaaa cgacatttga taacgatgta
     3241 cgtttgacga ctgacaatca aacagtagaa acacatcttg ccatccaaac aggaggcaaa
     3301 caatttgtca aagtggatcg gcaaacgaaa caaaaactag cagatgctca atttctagtc
     3361 aaaaaaaatg accaatactt agcgcaagaa aacgggataa atcattgggt agccaaagat
     3421 gattcaagag caacgatttt tacttccagt aaagatggaa cattttctgt ccatggattg
     3481 tcttttggaa gttacgaact agtggagatt aaaccgccta aaggatatat attgagtcaa
     3541 tcaaccattc tctttgaagt tgaaaaaggc agttatgatc cgcttcatcc tataccattg
     3601 gaaatcatca acgaaccaaa agcaaatccg acgaataatg gaaaaacgcc agttcagatg
     3661 cagacaaatg gtccatcaaa tgaaaaaaat ggaggaagct ttccaaaaac aagcgaagag
     3721 atgcttggag gtttctcaat tttagggttg ttgctagtcc tttcaacagg gacagcttgg
     3781 ttttataaaa aacaacaaaa aggaaacaat aggagggaaa taaaatgaaa aacatcaaaa
     3841 caaaaatatg gacgttttta ggaactgcta tcatgctgct tcctttcgtt ctcggcctag
     3901 gaactgcaga ggtttctagg ccgtatcacc tacaccagaa aatgttacgg ttaatttgca
     3961 taaattgaaa ttcacttcag caccagaaaa tcaaataaac aacggaacgg aattgacatt
     4021 tccgaatagc gaaccgttga acggcgttga attcaacgta tatgacatca ctgctacgta
     4081 ctatccaagt aaagacacag cagttccagc agacgctaca cccttcgcaa gtgtaacgac
     4141 ttctggtgaa ggattagcta acctgactct tccaggtaaa tctgatggga aagatgctgt
     4201 atacgtcttt gtagaaacac caaagcccgg tgtcgaaacg tcgccaaata tcgtgttaag
     4261 cttacccttt tataacccga attcatcaga ccctgacaaa cctttagaca cgattcacct
     4321 atatgctaaa aatgtcatca aagaaagcaa tatcaaaatc cagaaagctg ggaacttttc
     4381 aggggtagat aagttagcag gggcgcaatt ccagatcaaa aattcagaga atcaattcgt
     4441 gaccggtttt gatgaaaaca ctggtgtcgc tctctatgat cctgctgagt cagcgttgac
     4501 attcacaact gatactgaag gatccatcag tgtcaatgga ttaatagacg gcaattatac
     4561 tttagttgaa acaaaagcac cagatggtta tcaattaacc aatccacaaa aagaaactac
     4621 ttttatagta aaaaacggaa aattgcaaca aacaaccggt atcatccatg gcgcagatga
     4681 agccaatctt gggaatccaa acggtgtagt caatcgggaa aatacaacta cggataacac
     4741 gattactgta gaaaatgtcc aaaactacgg tgatttccac ttcatcaaac aagatgtgaa
     4801 cacgcaagaa aaattagccg atgcagaatt ttctattcta agagcaaaag atccaactgt
     4861 taaattaatg aaaaaaatag caaatcttgc ctcaggagaa tacccatatg cttggtcaga
     4921 tgaagtcaca ggttctgact gggaagaagt gaaattgatt tctgattcag aagggaaatt
     4981 ttcaatcgaa gggctgaaac aaggggcata ctgccttcaa gaaacaaaag caccagaagg
     5041 ctacgcatta cctaagaatg aagctgcgta cactgaattt acagtagatg atgatccaac
     5101 aacttctgat gaaacaacga tcgataacca acctaaagga atcttgcctt caactggtgg
     5161 tatggggatc atcgtattca tcttggtagg tactgcttta gttggaggag cagtgatcta
     5221 cttcaaaaaa cgccatcaag aaacagaagc ttaaactatt ggaaacaaaa atcatacgat
     5281 aaataaacga tagtgattca aatggggaga aaggttgtga ca
//
LOCUS       DAZUMV010000151         5275 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000151 DAZUMV010000000
VERSION     DAZUMV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.155"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACG8FK_002546"
     CDS             <1..546
                     /locus_tag="ACG8FK_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5098097.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
     gene            complement(716..1678)
                     /locus_tag="ACG8FK_002547"
     CDS             complement(716..1678)
                     /locus_tag="ACG8FK_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5098098.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1680..3119)
                     /locus_tag="ACG8FK_002548"
     CDS             complement(1680..3119)
                     /locus_tag="ACG8FK_002548"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS5098099.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3304..5250
                     /locus_tag="ACG8FK_002549"
     CDS             3304..5250
                     /locus_tag="ACG8FK_002549"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS5098100.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1693 a          907 c          885 g         1790 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac cgtaacagtt
      541 gtgtaaaaat atcattttag agcatgaaaa aaggagtgga tttctccact ccaaaaaagt
      601 ctaacttttg ggggtcacta caagttgaca actccttttt gtttacaaat caattggata
      661 tctacaaatt gtattttcta acgaatttta tagacattaa aaaattaatt taacgttaaa
      721 tacttcctcc agaaagtatt gagattggta aaatataatc cttatttgtc ttttcgcctt
      781 taattttttt cagaagaata tcaacaataa gacttgcaat ttcatcaatt ggttgtctta
      841 ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt accatcgtat ccaataattt
      901 ttaaatcttc tgggatatta agtttaagtt gatgagcaat tttcatagtg agaatggctg
      961 ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt tataatagtt gactttattt
     1021 ccatttcacg acggatagta gacagattat taggaatttt gaaaattttt ccatttggat
     1081 tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga atccgtgtta tcatttcctg
     1141 taatcataat ggtattttga caaccatttt tttgtaaagt cttagctgca agttttcccc
     1201 cttcaaagtt atctgaagaa acaatggaaa cattaggagc taaatttcta tcaaaagcaa
     1261 caataggtgc tctcacacgc tcataatcag caattccaag attatggctt gatgagatta
     1321 tgccatcaac ttggttggct tcaagcattt caatgtattc tctctcttta gcaggatcat
     1381 tttgactatt gcaaataatc gttttatatc catgtttaaa taactctatt tcgagatact
     1441 caatgagttc agagtaaaag atattactaa tattaggaaa aataagacca ataagctgag
     1501 gtgactttcc ttgaagtcca cgtgctaaat tatttggttt ataaccaaga gttttcatag
     1561 cttcatgaac tttttgtttt gttttttctg ataaatatcc cttattatta atgacgcgtg
     1621 aaacggttgt tgggctaact ccagctaatt cagcgacatc agttaatttt gctaccattt
     1681 taatatttta attcaaagta ttttccagta ggttcgcctg atttgatttg aataccattt
     1741 tggtgatttt cagggaatac acgactagta aacacttttt ctcctttatt aataaaaatc
     1801 tcaatgattg agttatctac aaaaatgtta gcagttgttt ctttagtatc aattgagcat
     1861 tcacgaatcg taccaaagtc agtggcatat tgaatgcctg ctttgctgcg atctaaaata
     1921 atttttcctt ctttggtatc tacaatcaag ctaagtccat ttcctttact atcagagaaa
     1981 agaagtaact ccgatttctg atttggagga aaagttagtt caagctcata agtattattt
     2041 gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta atgatgtaat agcttctact
     2101 ggatattggt aaagtttacc atcttttatt gaaagttctt ttacaagact cattgctccc
     2161 tgataatcat acttatcagt tggataatcg atatcaggta gtccaatcca actgacaatc
     2221 agagcacgac catcaggagt attgaatcct tgagtcgcat acgcttcaaa tccataatca
     2281 agattttgaa tttctgaagc gcccacaagt tttacttttt ctatgtcaag cgattgacag
     2341 atcttaaaag tattcggata gatattccca taatttagct cagatttatc caacccttga
     2401 ggacaataaa tgagaacagg ttttttgtct acaaatacta gatttggaca ctcaatcatg
     2461 tattctgaac cagtgccgcc aaaatcaagg ttgccaactt cctcccatgt ttccacgtta
     2521 ttgtcatccg ctttgtaaag tttgataaag cctgattttt ctaaattttg tgctccaaca
     2581 atcgcataca attgtccttc ataattaaac atttgtggat cacgaaaatg atcagttaca
     2641 tcagcaggtt tatgaataag aacattctca cctttcgtaa tgttgtagtc tttatccatc
     2701 catgctccga tttgaagtgt atcacgttcc caattttcat ttcgaacgtt tcctgtatag
     2761 agtaagaata gattcccctc tatttcatat gcagaaccgg aataagcgcc gtggctatct
     2821 cgttttgtgt ctggaagaag ttctgctcct gttacatgaa aatgaactaa atcatctgat
     2881 tcattgtgta cccattcttt caacccatga gcagctccaa atggccaatt ttgataaaaa
     2941 agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta
     3001 ggttcgatat gatagctagc acgccaaggt gatcgtttta cattatcctt aattttatcc
     3061 atctcatctt tagtccaatc agaataagca cgatatcgta tttctcgagg taagttcata
     3121 ttttactcct tcaatttatc tttttacaac tagtatggta aacgttttcc gttgaaaaat
     3181 caatcatttc atttaaaaaa ataaactata tgacaaacgt ttgacatatt tttaagatat
     3241 gctaaaataa atttgcattt cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt
     3301 cgaatgaatt acagtaaagt agcttccgac gtaatacaag cggtcggaaa agataacctt
     3361 attgcagctg cacactgcgc tacacgtctt cgtctcgtac taaaagatga cacgaaagtg
     3421 aatcaaaaag ctttagacga aaatccagat gtgaaaagca catttaaaat tgatggacaa
     3481 tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa
     3541 actggacttt cggaaatttc aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa
     3601 ttcaacccca ttatggcatt aattaaatta ttatcagata tttttgttcc aattatacct
     3661 gcccttgttg caggtggttt attaatggca ttacaaaact ttttaacagc tgctggactt
     3721 tttggaccaa aatcaattga agaaatgtat cctgcgattg aaggtatttc ctcaatgatc
     3781 caactgatgt ccgctgcacc attcatgttc ttaccaattt tagtaggtat ttcagctgct
     3841 aaacgttttg gagcaaacca atttcttggt gctgcaattg gtatgatcat gacaacacca
     3901 gatcttggtg gctcaactga atattggaat atttttggtt atcatgtttc acaaacaaac
     3961 tatgcttatc aagttattcc agttttagca gctgtttgga tattatctat attagaaaaa
     4021 ttctttcata aaaaattgcc atcatctatt gactttacat tcacgccatt attatcagta
     4081 atgattactg gatttttgac tttcacagtt attggccctg taatgttatt gttgtctaat
     4141 ggaattacag acattattgt ttggctatat aacactactg gatttattgg aatgggtatt
     4201 tttggaggaa catattcact tatagtcatg actggtttac atcaatcatt tccagctatt
     4261 gaaactcaat tactttcagc gtggacaaat ggtatcggac atggagactt tatctttgta
     4321 gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat ttgccatttg gttcttaact
     4381 aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg tctcagcact tcttggaatt
     4441 acagagcctg ccttgttcgg agttaactta aaataccgct ttccattctt ttgtgcttta
     4501 attggttcag gaattgctac agcagtagca ggattactta aagtagttgc tgtttcactt
     4561 ggttcagcag gtttcttggg atttctttca attaatgcaa catctattcc attttacgta
     4621 ttgtgtgaat tgattagctt tgtagttgct tttgcaatta cttacttcta cggtaaaaca
     4681 aaagctgctg gtatctttaa agcagaagaa attgctgaac aaacaccagt taattcttca
     4741 aaagtagcaa caacagatcc aatatctact gatgttgaat caacacaaac aaaagtttca
     4801 tcagaaacaa ttgttagtcc catttctggt aaagtgatta gtctttcttc agtaaatgat
     4861 ccagtctttt catcagaagc tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg
     4921 gtccattctc cagtagatgg tactattcaa ctcacgtttg aaactggaca tgcttatggt
     4981 attaaatcaa ataatggtgc tgaagttcta attcatattg gtattgatac tgtatcaatg
     5041 ggaggaaaag gatttacaca aaaagttact actaatcaaa aagttagaaa aggcgatatt
     5101 cttggagcat ttgaccgttc aatgattgag aaagaaggtc ttgatgatac aacaatgatt
     5161 attgtgacca atacttcgga ttattcagaa gtaaatatag tagctgaaaa tacagtcact
     5221 gagggtactg atttacttga aatcaaataa atatatttcc ctgagtaaag atgga
//
LOCUS       DAZUMV010000152         5212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000152 DAZUMV010000000
VERSION     DAZUMV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.156"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..361
                     /locus_tag="ACG8FK_002550"
     CDS             5..361
                     /locus_tag="ACG8FK_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFS5098101.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            377..727
                     /locus_tag="ACG8FK_002551"
     CDS             377..727
                     /locus_tag="ACG8FK_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFS5098102.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            756..1535
                     /locus_tag="ACG8FK_002552"
     CDS             756..1535
                     /locus_tag="ACG8FK_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFS5098103.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1546..2223
                     /locus_tag="ACG8FK_002553"
     CDS             1546..2223
                     /locus_tag="ACG8FK_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFS5098104.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2242..3348
                     /locus_tag="ACG8FK_002554"
     CDS             2242..3348
                     /locus_tag="ACG8FK_002554"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFS5098105.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3338..4429
                     /locus_tag="ACG8FK_002555"
     CDS             3338..4429
                     /locus_tag="ACG8FK_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFS5098106.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4442..5182
                     /locus_tag="ACG8FK_002556"
     CDS             4442..5182
                     /locus_tag="ACG8FK_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFS5098107.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1664 a          861 c         1247 g         1440 t
ORIGIN      
        1 aatcatgatt aaagtagtta ttgcagaccg tatgggtaaa ggacaaaatg tggccaaagg
       61 ggtagaagca gctggtggaa aagcagttgt cgtaccagga atgggggcag atatgcgttt
      121 gggcgatgtg atgcaacaag agcatgcaga tatgggtatc tctttttgtg gtagtggtgg
      181 tgcaggtgca ttaacagcag caacaaaata tggctatcca gaacgacatg gtatgcgttc
      241 gattgaagaa gggatcacag ctatcaagga tggcaaaact gttctaggtt tcggctttat
      301 ggatcaagag gaattaggga aacgtctgac agaagaattt atcaaaaagc atggaaaata
      361 gaaagaggga catgaaatga gcgttcaaaa gacaattcag caaaaagtta tcgtctccgg
      421 taaaggggaa acaaagcaac atgcttttgc atcagcgtta gcagaaattc aaaacaaaat
      481 aatggaacaa tcaaacgtat tattgcgaat cgaaccagtc aatgtggata ttttaaaggc
      541 ggtcgaaaac aaatacacag aacgctttct atttttcttc ttcccacggc aacgtattag
      601 ctatgaagtc gaactctctg ttactgtggc tgtaacagaa ataaaattgg cagacatatc
      661 gttcagccag caaatgactg aagatccaaa tgggattgcc attccatttg taactaaaaa
      721 aatctaattt tgaatcataa gggggaaaga ataacgtgga tttcgtaatc attctagtca
      781 aatcggtcat tatcggggga ttacttggtt ttgcagcatc tgtcggggca gctcgaatgt
      841 ttcatgcacc taatacacaa ggtttaggag cttttagaac attaggcgaa atgaatgcct
      901 gcatgggtga tccagcttct catttttcat ttggtttagg ctttttcttt aatgcatggg
      961 cttcttcagt tggtgctggt gcattcacac aagatgtgac gcatcgaatc attcccaatt
     1021 gggcagcggc agcacttcta gcaaaaaaca aagatgtcac ccagaccatg catgatccga
     1081 aaaaaatggg gatcattgga gcggttatcg gggcagtggt ggtagccttc ttaaacacaa
     1141 cagctgcagc aattccagag gcgttacaag tgacggcggt ttccgttttg gtaccagcag
     1201 cgacgattct gatcaataca gtaatgccag ttattttttg gttggcagct ttagacgcag
     1261 ggaaacgaac tggtttttgg ggcactcttt ttggtggtat cgcgcaattg attatgggaa
     1321 atgcggtacc tggtgttgtt ctaggaatct tgattggtaa aggcgtcgat gagagcggtt
     1381 ggaatcgcgt cacgaagagc atgatgatcg ctattgtttt attatttatt ttcagtggat
     1441 ttttccgtgg atttgatatg gaaatgatca agagcttcca gcttcaagtg cctaattggc
     1501 ttgataactt gcacaacatc tttagtgtga aataggagga gaatcatgga gacaacagaa
     1561 gaacaagcaa aagtattaga agcaaagcgg caaaagagct tttggtttgc agactggtct
     1621 tttccgctaa tcgtaggaat tatgtccgct gctgtatttg caggaacaca catgtatgtg
     1681 atgtatggtg tgggtgcatt caatgaagtt tcgatcgtgg cgatgttaaa agccgggctg
     1741 gatggcggat cctatggcgc agcggcagcg tttggtgcta gtttcttatt tgccagaatt
     1801 ttagaaggct ctttagttgg gatcttggac ttaggcggtt cgatcctaac tgggatcgga
     1861 attggggtac cagcaattct cttaagtatg aaaatagcgg caccaatcga taattttgca
     1921 ctctctttat tgactggttt ggttctaggt ttgattgttg gtggcatcat tgtcttgatt
     1981 cgtaaattta cgatcaatca atcaaattcc acttttggtg ctgacgtcat gatgggggca
     2041 ggaaattcat ctggacgttt tttgggtcca ttaattatat taagcgcgtg tgcagcatct
     2101 attccaatcg gcattggcgc tacattagga gcactaggat tctatgcttg gaaaaaacca
     2161 attgctggag gtgctatttt aggagcaatg attttaggag caatcttccc ggcagtaaat
     2221 taatagaaag agggtcatta aatgtttgat actttgatta aaaatggaag gttagttgat
     2281 ggatctaaaa tagaggtcgc tatagaaaaa ggcatgatta aagcagttgc agcagaaata
     2341 aatcagccgg cgcaagaagt agttgacttg gagggaaagt attatttatc cgctgggtgg
     2401 atcgatgatc atgtccactg ctatgaaaag atgaatcttt attatgacta tcctgatcag
     2461 ataggggtcg aaaaaggtgt gacgacggtc attgatgctg gaactacggg agcagaaaac
     2521 atccgtgaat tttatcaatt gacgaaatcg gtcaagacaa atgtgtatgc gctaatgaat
     2581 atctctaaat ggggcattgt caaacaagat gaacttgctg atctaacaaa gattcaagaa
     2641 gatctagtaa gtactgcgtt agcagaactg ccggaattta ttgtcgggat taaagcgaga
     2701 atgagtaaga cagttgttgg tgaaaatggc attaagccat tagaattagc taaaaaaatc
     2761 cagacagaaa acaatgatct gccattgatg gttcatatcg gttctgcacc tcctgagtta
     2821 gaagaagtat tgtcaagaat ggaaagaggc gatgtattga cccattgttt taatggaaaa
     2881 ccaaacggta ttttagatcc aaagacagat caaattaaaa actttgtctg ggatgcttac
     2941 aataatggtg tcgtctttga tattggacat gggacggata gctttaattt ccatgtggca
     3001 gaaaccgcac taaaagcagg aatgaaagct acctctatta gtacggatat ctacatccgc
     3061 aatcgtacaa atggcccagt ttttgatcta gcaactactt tagaaaaatt gagggtagtc
     3121 ggctattctt gggaagaaat cattgaaaaa gttacagcag ttcctgcacg gaattttcat
     3181 ttgaagaata agggccagtt agctgtcaat tatgatgcag atttgacgat atttgagatt
     3241 gttgaaaatg aaaaggaatt gaaagattcc aacggattta cacgtatagc taaagaacaa
     3301 attttaccga ttaaaacaat tattggaggc gtcatatatg acaattagct atggaaagtt
     3361 tgatttaaaa gaagtcatta atgcatcggg aaaaatgacg atcctagggg tttctaaggt
     3421 atctgaaagt gtcctagcag cacaacgatt tggaggcgag catttttttg agatgagtga
     3481 tcttgccatc aaaaccggcg cttatctttc tcaattactg aaagtggaag atgcgcaaat
     3541 cgtttcttct gcttcagcag ggattgctca atctgttgct gctttaatcg gacagggcga
     3601 catgtaccat gtctatcatc cctatacaga aaaaatcgct agacgggaaa tcatcatacc
     3661 taagggacat aatgtagatt atgggacacc ggttgaagta atggttgctc aaggtggagg
     3721 aaaagtcata gaagctggct atgcaaatat gtgtacaccg gaacatgttt ccatgatgat
     3781 tacggaaaaa actgctgcgc ttctatatat taaaagtcat catgcagttc agaaaagtat
     3841 gctgacggtg gcagaaatgg tagaagttgc caaagctcat cgtttgccat tgatcgttga
     3901 tgcagctgcg gaagaagatt tattcaaata tagcgaaatg ggcgttgact tagtgattta
     3961 ctctggggca aaagcaatcg aaggtccgag tgccggactg gtcataggaa aaaagaagta
     4021 tattcaatgg attcggctac agtctaaagg tattggccga gcaatgaaaa tcggtaaaga
     4081 taatatttta ggctttaccc aggcactaga agattattta aaaaatggga gcgaatccgg
     4141 tgactccatg aagcaacgat tagcgccttt tgttaacgca atcaatcaac ttcctgacct
     4201 ttcagcgaaa atagttcaag atggtgccgg cagagatatt tatcgagcaa gtgtaacgat
     4261 taatggcgat aaaagtgcga aagaagtcat tcaggaatta aaagcacgaa atccagcagt
     4321 ctacacccga gaatatcaag caaataacgg aatcattgag ttcgacattc gttccgtcag
     4381 ccaaaacgaa atggaaaaaa ttataaaaag attgagagaa atcatgtagg aggcttttaa
     4441 attgtcagtt atcccaaatt attataaaaa tcgtgtatgt ttaaatgtat tagctcattc
     4501 agttgaaaat gcaaaagcat gctatgaagc agcggaaggg catgtcgtac taggtgtgct
     4561 atcgaaaaat tatgctagcg atgaagcagc aatcgaagat atgaaaaggt accaagacgc
     4621 aaccaataat gcgttgtcag ttggattagg cgctggagat ccaaaccaaa gtcagatggt
     4681 ttcgcgttta tctggggttt tacaaccaca acatgtcaat caagtattta ctggcgtggg
     4741 gacttctcgt gctttattgg gccaaaatga tacagtcatc aatggattgg tttcaccaac
     4801 aggtaagatc ggctatgtca atattgctac aggtccgtta agttcacaag cgccagcagc
     4861 tgaagtgcct attgaaactg ccattcaaat gctgcaagac atgggaggta cttcaatcaa
     4921 gtatttcccg atgaaaggct tagcacatag ggaagaattt aaagcagttg cggaagcttg
     4981 cgcaaaatat gaattctatt tagagccaac aggaggaatt gatctttcaa actttgaaga
     5041 aattctgaaa attgcaatcg atgcaggtgt aaaaaaagtg attcctcatg tttatagttc
     5101 aattatagat aaagaaacag gagatacgcg tataggagac gtaaagatac tattaagcat
     5161 ggtaaaaaaa attattcact aactcattag aaaataagaa gcttattcca ga
//
LOCUS       DAZUMV010000153         5165 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000153 DAZUMV010000000
VERSION     DAZUMV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.157"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(591..791)
                     /locus_tag="ACG8FK_002557"
     CDS             complement(591..791)
                     /locus_tag="ACG8FK_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5098108.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(925..1533)
                     /locus_tag="ACG8FK_002558"
     CDS             complement(925..1533)
                     /locus_tag="ACG8FK_002558"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS5098109.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(1618..2088)
                     /locus_tag="ACG8FK_002559"
     CDS             complement(1618..2088)
                     /locus_tag="ACG8FK_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS5098110.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(2136..3050)
                     /locus_tag="ACG8FK_002560"
     CDS             complement(2136..3050)
                     /locus_tag="ACG8FK_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5098111.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3196..3999
                     /locus_tag="ACG8FK_002561"
     CDS             3196..3999
                     /locus_tag="ACG8FK_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS5098112.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            4177..5124
                     /locus_tag="ACG8FK_002562"
     CDS             4177..5124
                     /locus_tag="ACG8FK_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS5098113.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         1663 a         1013 c          791 g         1698 t
ORIGIN      
        1 aagatcgaaa gaccgaatac acgcagttcg caatcgtttt atcttttttt atgtattttt
       61 tccagaaaaa gcgaacaaca aggtattttc caaataatcg tgcagtaaaa tgatcgcaca
      121 tacctttccc atctttttaa cagtctttgc ctaccgttca gattatcatt cgatttcacg
      181 acaatttctt cctatgactg gtccttttgt taaacccatc ttctattcgt tttatttcca
      241 gctcactgca tggctcttaa aatgactcga actagtgatt gcattaccat catcttttaa
      301 tggatagata tgctcctctt caaagtaaaa cgattttctt ttaaagtaat agaattgaca
      361 tggtaatgag tatgtatact agtggtttca caaatggtct tttattcaac atccaaaaac
      421 tgaaatttct ttaaaatcca ttactaacga agaaaatgtt ccttgcttga taattagtta
      481 ggacatgtca agtgaacaaa tgcatttact tagccaaaaa atctaaaaac ttcataaata
      541 gacaaaaaga cacgactctc taagaagtcg tgtctttatt caacaaatta ttataattta
      601 gtcacttcag cagcttgagg tccgcgtgca ccttcaacaa tcgtgaattc tacttcttga
      661 ccttcttcta ggctcttgaa gccgtctcct tgaattgctg agaaatgaac aaatacgtca
      721 tctccgccgt ctactgtaat aaacccaaat cctttttcat tgttgaacca tttaaccgtt
      781 ccgtgttcca taaatgtata gcctccaaaa aagttcttgc aagtaacctt gcaagaacat
      841 tatacccaat ttagaaaacg ctagcaagct ttcgctcttt tattttccaa attattttta
      901 agactgatat gacggaggaa atggttattt tgctgcttcg aaacgtttgt ttacttcatc
      961 ccaattaata acattccaga atgctgcaat ataatctggt cggacatttt tatatttcaa
     1021 ataataagca tgttcccaaa cgtccaaacc tagaattggt gtctttcctt ccatcaatgg
     1081 agaatcttga tttgcagtag aggtaatagc taattttcca ttttccatta caagccaagc
     1141 ccatccagaa ccaaatcgtc cagcggctgc ttttttgaat tcttctttaa aagaagaaaa
     1201 atcaccaaaa gcttcattaa tcgcttcttt tatttctcct gtaggttcgc caccagcatt
     1261 tggtgccatg atttcccaga aaaatgaatg gttagcatgt ccgccaccat tgttacgtac
     1321 agctgtcttg atatctgttg gaatagcgtc catatcagat aataattctt ctattgtttt
     1381 ttcgcctaat tctgggtatt tctcaattgc tgcatttaaa ttcgtcacat aagtattgtg
     1441 atgcttatca tgatgcagat gcatcgtttc ttcgtcaata taaggttcca atgcgtcata
     1501 tgcataaggt agatctggta atgtgtaagt cataaaaaaa ttcctcctca atgttttctt
     1561 aataatgtct acattacaaa gagtaacaaa gagaaggaaa ttcttcaatt gttatgccta
     1621 gtcgtctttt tcactttgac ctacttcttc ttcaaaaacc tcagtttctt taaaaataaa
     1681 caacttgctg aaaatatagt tcgcaaggat aacaataatt tgagtgatga ttttcgccac
     1741 atactcattg ctgttcataa cattgatcaa caataccatt gcccccatgt ccaatccgta
     1801 agaaaggata cggtagaaga agaacttacc gcattcaata agtgcatgtg taaaattcgg
     1861 tgattttgaa tggaataccc aaagtttgtt cgttacaaaa gcaaacaata cagaacaaaa
     1921 ccacgaaata gaattggata aggccatact taaatcaaaa tattggcgtg cgagagcaaa
     1981 actaacaaaa tttacaatcg tagccaaacc accaaaaaag agatagacta gtatctccca
     2041 atatcctttt gcttctaaat atccttttaa ctgtcgatac tgcttcatga gccaacctac
     2101 cttattaaaa attcttattt atcatactct atatttcaat aactttcatc tttatttaaa
     2161 aaaattaatt ttttttgatc ctttgccttt gtctctgctt ggatcgtcac atgtgtaata
     2221 ccatatttta attttaataa agaaattata cgaggatata tcttttcaat ctcgcttagt
     2281 agctgatctt caaaatcaat atgtccgtcg aaaataatta tcttttcgct atactgccaa
     2341 atatggacat gatgaacatt ctttacccca tcgattgctt caatttcttg ttccattttt
     2401 tcatagtcaa tagcaggtgc actttgtatc aaaatagcgg cagctttttt gatcaccatt
     2461 atcgcttcta tcaatatata tatcgaaatg atcagtgtaa taactggatc gatccagtag
     2521 atatcgaaaa aacggatcaa aattgctccg ataatcacac ttaccgaaga cagtgtatca
     2581 cttaacaaat gcaaataact agattttata ttcaaacttt catctgcttc ttgtcgtaat
     2641 aataatactg agaacaaatt cgcgagcaat ccaataactg ctactgttag catcaaatga
     2701 ctatttatct tttctggtga agtgaaccgc ttgaatgctt caacggttaa aaacaaggat
     2761 aatatgatca atatgaatga gttgatgaaa gcactcaata tttctaaacg ctggtagcca
     2821 tacgtacgtt ttttatttgc ttcttttcgg gccatttttt gtgccccgta agaaaaaata
     2881 attgccaatg tatcactaag gttatggaaa gcatctgaaa caagagacaa actccctgac
     2941 ataatccctc caataaattc tgataaagtg atcaccgcat tcaaaaccac tactccaaat
     3001 agtttttttg atgatctctg tggctgtgta ggcactattc cttcctccat tttatttata
     3061 ttggatagtc tttagacaag aataacattt ttttgatatt tcaccaatca ttcagatgtt
     3121 tccctaaagc acttgtattt cacttgatac ttcgctagaa ttgcaagtga tgacactact
     3181 agatgaggag tacttatgac aacaaaacaa ttaattatca gttcctttat acgattcaaa
     3241 gacctaaagg agacgagaaa tgtcccattt tggaaagcca ttgtctatct tttagttttg
     3301 agtataatta tggctttgcc aatctctttt caagtttttc aagtcttaaa caacattcaa
     3361 ttggatgggc aaaagattgc tactcgcatt cctgatttta caatccaaaa cgggcagctt
     3421 caaaccgaag aaaaagaagg attcatttac caaacaaact ccattatctt cacatttgat
     3481 ccagagggaa aacgaacaga acaggacatc tctactgatt tgatggggaa ttttgttagt
     3541 gtcgggatgt taaaaaacaa acttatcata gctcttccta atacaggcac gacttctgct
     3601 ttgttgaaca ataaccaatt agaacttccc tacaccaatg aatctttaaa aaacttaaca
     3661 ggtaaggagc tccgttcttt tttaagtaaa gcatctatac cactttggat aaaagtcctg
     3721 acatttatat tttctattta tcctagtttt ctgaatctgc tgatcacttt gctatttacc
     3781 aacattgctg cttttctcta tgcacgcttt cgactaacaa aagctacttt tcttgattgt
     3841 ttaaaaacat tgatctattc aatttcattg cctactataa ttgcaacaat cttgatggtc
     3901 tttcttcctt cttttgacac aagcgccttc attgcttttg caggaatctt tatttttgct
     3961 caagctgtca aaggatgggc aaaaatatcg atcaattaag aaataaagac aactcgttat
     4021 ttctacttca atagaccctt ttagaggttg tgacattttt ttgtcacagc ccctttttta
     4081 tttgaaaaat atttctttct caaaaaacaa gcaaggcgct tcaaagttct tcttgtatgc
     4141 gctacactta gagtaaagaa aggcgggaaa ttaactatgt cagaaagaat catttatgca
     4201 gatcgtccat ttcgtgatga atttatcgaa gaaatccata ctatagcgcc tggttatatc
     4261 tttaaaacag aattacaacc agaggatcta caaaatgtag agatcagttt gggctggaac
     4321 tcagactacc aagaaaaact attagcctct tctaccttga aatgggtcca atctatctca
     4381 gctggtgttg atcagctacc ccttgagact ttttcaaaga aacatatttt actttcaaat
     4441 ggtagtggca tccacagtga atcgattacg gagcatgtct taggaatcat ccttgggtat
     4501 tctcgtggtc tttttcaagc acaaaaagca cagagtgaca tgaaatggtt aggttcatcg
     4561 gtccattacc aatctatcca aggaaaaaaa atactaatca ttggaactgg ccatattgga
     4621 aaaatgattg caaaaaaatg caaagcatta gacatgaact gctatggaat caatacatcc
     4681 ggtcatccag cagaaggaat ggaaaagact ttttctttaa atcaactaaa ggaagttctt
     4741 cctgaagtag atttcgtagt caatatctta cctctaacgg aggaaacaac tgggctcttt
     4801 gatcagcata tgtttgaaca ctttgatcca aaatctgtat tcatcaatgt tggccgtggt
     4861 gcttctgtta agacacaaga tctggtgcag gctttaaata accagcaact ttcctttgca
     4921 gcattagacg tttttgagga agagcctctt ccacaagatc atcctctttg gaaaatggac
     4981 aatgtactga ttacttccca cattgcaggt ttgacacctg attttcaaaa gaaattaatg
     5041 gctatttttt taaccaatct gaaaagctac tttgccaccc atgagcttca aaccaatcaa
     5101 gtaaaattgt ctgcaggata ttaatagctg aaaacaatat tttcaaaaga acaaagagaa
     5161 ggtga
//
LOCUS       DAZUMV010000154         5159 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000154 DAZUMV010000000
VERSION     DAZUMV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.158"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..740
                     /gene="treR"
                     /locus_tag="ACG8FK_002563"
     CDS             27..740
                     /gene="treR"
                     /locus_tag="ACG8FK_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS5098114.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            817..1263
                     /gene="msrB"
                     /locus_tag="ACG8FK_002564"
     CDS             817..1263
                     /gene="msrB"
                     /locus_tag="ACG8FK_002564"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS5098115.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            1433..2035
                     /gene="ruvA"
                     /locus_tag="ACG8FK_002565"
     CDS             1433..2035
                     /gene="ruvA"
                     /locus_tag="ACG8FK_002565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS5098116.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            2048..3049
                     /gene="ruvB"
                     /locus_tag="ACG8FK_002566"
     CDS             2048..3049
                     /gene="ruvB"
                     /locus_tag="ACG8FK_002566"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS5098117.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            3078..3656
                     /locus_tag="ACG8FK_002567"
     CDS             3078..3656
                     /locus_tag="ACG8FK_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5098118.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            3708..4190
                     /locus_tag="ACG8FK_002568"
     CDS             3708..4190
                     /locus_tag="ACG8FK_002568"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS5098119.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            4307..4681
                     /locus_tag="ACG8FK_002569"
     CDS             4307..4681
                     /locus_tag="ACG8FK_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098120.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         1770 a          872 c         1120 g         1397 t
ORIGIN      
        1 atggaagaac gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg
       61 aacagaaaat tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat
      121 tgatcaaaat ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta
      181 cggaaggtta tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct
      241 tcgactttcc tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc
      301 caagtgaaac gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga
      361 tcactggctg ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg
      421 gcgaagtagt tatattagac accgattatc tattaaagga aatcgttcct attttaactc
      481 cagcacaagc acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg
      541 cttacgcgca aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg
      601 atcttcacga tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc
      661 gctgtttcga gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg
      721 cccgacgccg aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt
      781 tggcgctatg ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa
      841 aaagaagaac tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca
      901 acagaacgac catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat
      961 atcgtcagcg gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg
     1021 ccatcattca gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat
     1081 ggcatgcatc gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt
     1141 accgatggac cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa
     1201 ttcattccat atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa
     1261 taatggaaat acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt
     1321 ttattcaagc gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga
     1381 aatgtgctac aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga
     1441 atatctgaca ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg
     1501 tatcggctac cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa
     1561 aataaaaatt tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga
     1621 ttcgctagaa gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa
     1681 aagtgccctt gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc
     1741 cagtgatgtc acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat
     1801 gatcttggat ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt
     1861 tgatgaagca aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc
     1921 tcttggttac agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa
     1981 tcagacaacg gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg
     2041 agaccaaatg acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt
     2101 ggaaaaatct cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca
     2161 agaacttcag atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct
     2221 gttgtacggg cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat
     2281 gaccgtaaat attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc
     2341 aatcttgaat gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc
     2401 tagagtggta gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt
     2461 aggacaaggg ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg
     2521 cgcgacgaca cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc
     2581 tcatatggaa tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat
     2641 ttttcaaaca gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac
     2701 gcctagaatc gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga
     2761 cgggaaaatc gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc
     2821 tggattggat tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg
     2881 accagtaggg ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga
     2941 tatgtatgaa ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat
     3001 tgctactgct tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt
     3061 agattggagg aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat
     3121 tgattgattt gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg
     3181 aagcaaaaat caatcgacaa acattttatt atcattttac agataaatac gatttgcttc
     3241 gttgggtcta ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata
     3301 attgggaaga gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg
     3361 caaatactgt gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa
     3421 aatcatttat caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca
     3481 aactatttta tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca
     3541 cgaatgaata tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg
     3601 atacggaatt atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga
     3661 aaaatttttt gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt
     3721 tatttgtttg tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac
     3781 gaatagcatt agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt
     3841 atgaagttgg aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag
     3901 atatgacgaa tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga
     3961 tcattggcat ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc
     4021 aggggaaaat ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg
     4081 acccatacta taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc
     4141 attggtggaa tatttggatt gatgaaaata aaataacaaa taatttgtaa agttaacgct
     4201 ttctttttaa ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata
     4261 aaattaatta agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac
     4321 agtatcgcca atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga
     4381 ttcttttgta aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat
     4441 tcttgagctg caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa
     4501 tcgtttgagt agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa
     4561 agaccgagtg atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga
     4621 acaaattgaa aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata
     4681 atttatacca gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa
     4741 taaccgaatg ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcatagc
     4801 atgacagcat tcggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa
     4861 aaggtcctgc tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta
     4921 gggttgtttg ttggtatgct tatataatag tcattagttg tgaatatttt gtgacgaaag
     4981 ttttcgaaag ttgttaagtt tttgagagta taaaatattt tgtgtaaatg aaaaaatcca
     5041 tacaaaaaag gaagtccctt ctgtagaata aagttaacga caaccaattc acagaaaaga
     5101 ggacttccct atgaatgatt ttactacaga aattgtgcaa actctagtca ctaaaggcg
//
LOCUS       DAZUMV010000155         5146 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000155 DAZUMV010000000
VERSION     DAZUMV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.159"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..1016
                     /locus_tag="ACG8FK_002570"
     CDS             141..1016
                     /locus_tag="ACG8FK_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5098121.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1120..1884
                     /locus_tag="ACG8FK_002571"
     CDS             1120..1884
                     /locus_tag="ACG8FK_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS5098122.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1871..3313
                     /locus_tag="ACG8FK_002572"
     CDS             1871..3313
                     /locus_tag="ACG8FK_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5098123.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIASLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3324..4655
                     /locus_tag="ACG8FK_002573"
     CDS             3324..4655
                     /locus_tag="ACG8FK_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS5098124.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            4668..5126
                     /locus_tag="ACG8FK_002574"
     CDS             4668..5126
                     /locus_tag="ACG8FK_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5098125.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1745 a          753 c         1091 g         1557 t
ORIGIN      
        1 attgataacg cttttattat gtgttaaact atttttgtaa attatgtagg gtgctactat
       61 tcaattaggc acgacctaag aaagggttct ttcttaggtc gttttttgtc tttaaaaatt
      121 gtttggagac aaaaatatgt atgaaggagt ctgcaagttg ttttgtttta tcatatagag
      181 gaggtgaatt aacggtgtat gtgatgaaaa agctgaatca taatgctgta ttgatcgatg
      241 acgatggaat agaaaaaatt gcgatgggga aagggattgg cttttctgct aaagtgagta
      301 agccatttga tgttgaggat gctgacaaat tatttgtatt agattctaaa gaaaagatca
      361 aacgattcag tgagatagtc tcgcagattc caatcgaatt tatggatttt gcggaagaag
      421 tgattgaact gataacagct aggatctcct ctccattaga ttcaaatatc tatattacat
      481 tgacagatca tatttatttt gctattcaac gaaaaagaga agatagagat gtcacagcaa
      541 ttatgttgcc agaaatgaga ttcctttatc caaatgaata tgatatagca gtagaagtgg
      601 tcgagcgaat caatcatcga tatcaaaccg agttagggtc aaatgaagta ggattcatca
      661 ctatgcatat cattaatgct gagctaggag agcctaatag tttaaagagc ttaaaaatag
      721 tagaaatgac gaccacgatt ttgtcccatc tagaaaaatc ctattcaaac gtttatgata
      781 aagaatcact gacttatcat cgcttaatgg ttcatatcaa attcctggtt aaacgcttga
      841 tttatcagga agaaacgcca gtagaaaata ttggtttttt tactgaaaca tttaaacata
      901 gcaaagcgta ttgtacagca ctaaagatta aagatatcat cgaacagcaa tttcaacttg
      961 ccattcaaga aaatgaaatc atctatttgg caattcatct agcaagaatc tattaaaata
     1021 agggcgtaac tatctatagg cgcaaccaaa tgacacatag taaatggact atgattattt
     1081 ggttgcgcct attttagtag aaaaaagaag gagaatgaaa tgggaaaagt gatttttaat
     1141 tcggatgatt ttggctatag ccatggtgtg aattatggga ttatggatgc ttatcagcga
     1201 ggaattttga catcaactac attgatggca aatatgccgg gtttcgagca tgctgtgaaa
     1261 ctaagaaagg aaatgcctag gctaggtgta ggagtccatt taacattgac gtgtggcaaa
     1321 ccactcctca aaactgtcga tactcttatc gaaagcggag agttcagatc cttatcttat
     1381 tatcagcatc catttaacat tgacaaagat caactgtatc aggaatggaa tgctcagatt
     1441 caaaaaattt atcatgcggg gatcatacca actcatttag acagtcatca tcatactcat
     1501 actttggatg aaaatcagga ggtagttgtt acattagcaa aaaaatatga tttgccggtt
     1561 cggagcaact ttgaacgaaa agatgaagtt cgtcatgtcg attattttga gccttgtttc
     1621 gatgtcgtag gagaaactga tgaaaaaaaa cgacaaatca atacgagtgt ggaagaatat
     1681 ttggcaagtt tactcatgat attgagagaa gataaaacga tcgaagtcat gtgtcacgca
     1741 gcgtatttgg atcaggaatt gttaaatggt tcaagttttg tgtatcctcg tacccaccaa
     1801 gtcgaatttt taattcattc agcatttgct caaaaagtga aaaatgatcc tttgattgag
     1861 ttagtaacat atgaggatat ttaatagagg aggaataaaa atgaatgtta aatcaaatgt
     1921 gcagaagctt ggaagagcga tgctacttcc agttgcagcg atgccattag cgggattgat
     1981 catgcgttta tcagctgacg atatgctgaa catcccagta attggtgcag ctggaaatgc
     2041 cgtttttgga aacttggata tcttatttgc tataggagtt gcaatcggtt ttgtcaaaaa
     2101 taaggacaaa ggaatacctg cacttactgg ttttttagcg attgctagct tgaaaaaagg
     2161 cttagaaatt atggacccag aagtgaatat ggggattttt ggtggaatta tttccggttt
     2221 agtagcggca tggacgtata atcgctttaa agaacaaaaa ttaccagtag ttttttctta
     2281 ttttgcgggc gaaaaattcc cattgactat ggtgatgatt ctacaaacaa tcgcagctgt
     2341 ttttttgggc tttatctggc cgctcatcca aattgggatt gataattttg caaaactgct
     2401 cgttgatatg ggagcattag gtgtggggat atttatgttt ttgaatcgtt tgctgatccc
     2461 ctttggactg caccatgtgt tgaatacgta tatttattac gatttaggaa gctatacatc
     2521 tcctagcaag gaagttttca gaggagagat gacacgcttt atcaacggag atcctcaggc
     2581 tggactgttt ttatcaggtt tttttgtagt aatgatgttt ggtataccag ctatatgtct
     2641 agctatctat aaagcagctt ataaagaaaa taaagagatg gtcaaaggaa tcatgggaag
     2701 cggtgcagga acttcattta ttgcaaatat cactgagcct gttgaattta gtttcatgtt
     2761 tctttctcct atgttgtacg tgatccatgc actttttgcc ggtttagcag ggattgtctg
     2821 ttacttattc aatatacgaa ttggttttac attcggagca tgtattgttg attatctcat
     2881 caattttcga atcgctacca atgctatttt gattcttcca atcggaattt tcttttttgc
     2941 cttgtattac gttacttttt attatttgat caataagaga aacattcaga cattaggacg
     3001 tgaagctaag gcagagtttg gcaatgaagt aaccttagaa gaaacagagt tagggttagc
     3061 aagtaaaaat tatgaatata tggcaacaaa aatgctacaa gcgtttggag gaaaagccaa
     3121 tatcctggat gtctatagtt gtaatacacg cctacgggta gaggtagtgg atcccaccat
     3181 ggtcgaggaa cagcgaatca agcaattagg gataagcggg atcattaaac caactgaaaa
     3241 aaattatcaa attatcatcg gcttagaagt gacttatgtt atggcagaat tcaataaatt
     3301 attagaagaa tagaaaggaa aagatgatga tgaaaaaaca gattatcact atagctggag
     3361 gcgggagtac gtatacacca ggaatcgttc aagccatttt gaacaatgaa aaacggttgc
     3421 cattatctga aatacgttta tatgatattg atgaagaaag aaatatggat atgtacttga
     3481 ttgttaaatt tatgctgaaa agaaaaggtt tttcaaatat tcgcattcgt gcaacggatg
     3541 atccaaagct tgcttttaca ggatgcgatt ttgttttctc acaaattcgt gttggaggat
     3601 tggagatgcg agaaaaggac gaaaagattc cacttaaaca tggattagta ggacaagaaa
     3661 cgtgtggact cggaggattt gcttatggga tgcgctcaat caaaggttta ttggaaattg
     3721 tcgatcatat acaagactat gcccccaaag catggatttt gaattataca aatccagagt
     3781 cgatcgtttc tgaagctgtt cgtcgtaaat atccgaatgt aaaaatgatt aatgcctgtg
     3841 atatgactat ttcaattgaa gaaacgattg cagtgaatta cggttatgat cgtaagaatt
     3901 ggatcgttac gtattatggt ttaaaccact ttggctggta tacatcgatt tatgataaag
     3961 aattgcaacg agaaattatg ccagagatta ttgaaaaact aataacaaaa gaaatgcaag
     4021 tcgccgattt taatatagga gataaaactt ggcaaaaaac ctttcaaatg atgtcagtca
     4081 tcacgaaaaa ttttccaagt aatatcccga ataattactt agagtattac ttatatcctg
     4141 atatggtcgt tgagcatact gacaaagagt atacacgtgc taatatggtc atggatggac
     4201 gagagaaaaa taccaaagaa atggctgata aaatacgccg cggtattgtg gaagaagtat
     4261 tgaactttaa ttttggggag catggacaat atatcgtcga tatagctatt tccttgttga
     4321 atgatgatcg tcgtcgcttt atgctaatcg ttcctaatca aggggcgatt ccgaatctac
     4381 ggtcagatgc agttgttgaa ataccggcat atgttggtgc aactggtgtg gagccaatta
     4441 cactaagaaa accaatcagt gattttcata aaggattgat ggaggcacaa gttgcagtag
     4501 agaagttact agtagatgct tactttgaag gttcttatca aaaagcttta caagcattta
     4561 cattgaatca aaccgtacca aatgccagag tcgcaaaaaa aatactagat gaaatgatca
     4621 aagtaaacaa aaaatattgg ccagcattaa agtagaggtg ataaaaagtg catttcttta
     4681 aaacaagaaa cattctaaaa gctgtagcta gtggtcaaat tattccatta acagcagtaa
     4741 aagacgaggt cttttcttca aaaatgatag gagttggata cgctataacg aatcatgatg
     4801 gatatatcca tgcgccagtt aaaggaataa tcgataatat atttcctacc cgacatgcca
     4861 taacgatcga aagtgaatct ggcagaaaaa tactgatcca tatgggttta gatacagttg
     4921 aactcgaagg aagtccgttt cagatacggg ttgaaagtaa ccaggaggta aaagaaggga
     4981 cactgttagc tattatagat ttgaagaagt tggagcaagc acaaaaagat acggtgatca
     5041 ttgtagtttc tccaggcatt tttgaaggaa atttattaaa acaaaaccaa gaagtaaaga
     5101 ttaaagatag ttgttttgaa ttttagtgta cgatgaaaaa agttaa
//
LOCUS       DAZUMV010000156         5003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000156 DAZUMV010000000
VERSION     DAZUMV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.160"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..456)
                     /locus_tag="ACG8FK_002575"
     CDS             complement(<1..456)
                     /locus_tag="ACG8FK_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5098126.1"
                     /translation="MRCQQHLTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQM
                     TTVISVFGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLY
                     GIGDCIFTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYR"
     gene            complement(496..1548)
                     /locus_tag="ACG8FK_002576"
     CDS             complement(496..1548)
                     /locus_tag="ACG8FK_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5098127.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1561..2499)
                     /locus_tag="ACG8FK_002577"
     CDS             complement(1561..2499)
                     /locus_tag="ACG8FK_002577"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5098128.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2950..3600)
                     /locus_tag="ACG8FK_002578"
     CDS             complement(2950..3600)
                     /locus_tag="ACG8FK_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098129.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(3700..4209)
                     /locus_tag="ACG8FK_002579"
     CDS             complement(3700..4209)
                     /locus_tag="ACG8FK_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS5098130.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(4572..4955)
                     /locus_tag="ACG8FK_002580"
     CDS             complement(4572..4955)
                     /locus_tag="ACG8FK_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5098131.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
BASE COUNT         1592 a         1040 c          731 g         1640 t
ORIGIN      
        1 acgataaagc agaacaagta acaatgggaa aagtagaaag cctgcatagc ctagcccaga
       61 acctgtatta tggatccaag tcgatagatt ccaagagctt tcattcgtat tgatactgaa
      121 taacccagta aaaatacaat cacctatccc atataaacca actgttgcaa cagcaagtat
      181 tgcaagtgtt tttgatgttc caacaaatag gtgatacaac gcaggcaacg aaagaacaaa
      241 aaaaagtcca gatacaactg accagaccaa gaaagcacgt ctgactggac tatctacgtc
      301 accaaacaca ctaattacgg tagtcatttg attcagtttg ggataaaaaa ggccaaggat
      361 atacggtgtc gataaatcac taatcacacc taataataaa aaataaaaac catattttgg
      421 caaaaaaacg taagtcagat gttgttgaca tctcacttac gcatccattg attgaaaatt
      481 tcttagtaaa atctactagc ctaaaaattt gaagacttct tctcgatcat acagtttaaa
      541 tactttctcg cataaaaatt gagtaattag tgccgcaaaa acagggatga ctaaccaaca
      601 aagaacaaca agaaagccat ttagccctgc atctaaagaa gccaacggac caacaagtcc
      661 gaccacaccg aaaccagcag aagcaggcgt acctgaaata gaaaatagag cgacaggaat
      721 agctgtaatc actgcactaa ctaaacaagg aataagaatc acaggttttc taaatagatt
      781 aggcatcatc atcttcatag cgcctaaagc aatcgcaatt gtcacacctg acttattaac
      841 cttccaagag ttcactacta aaacaaaagt ggttgcagct acgcccatcg cagccgcacc
      901 agcagaaaca ccatctagtt ggattgccat tccgattgct actgttgaaa taggagagat
      961 aatcaaaaag gcaaaggaac aaccaatcaa gatagacatt aacagcggtt gtaaggtagt
     1021 gaagttatta atcaatgatc caattgcaga agtaattgct gttacaaaag ggaataaata
     1081 aaaaccaagt aaacctgcac ctacacctac aacaatcggt gttaacacaa tcgacactga
     1141 cccaaaacta tttccaatca gtaaaatcag cccgacagca atcgatgctg tcagcatcgt
     1201 attaataata tcgcctgtac cagcaccaac gtaaacaccc gtttcaccca aatcaggtgt
     1261 gaatttgact acaccagaac caacaaaaga agctccggct acaaccatca ttctcatcgg
     1321 atcaaatcca aattgtaagg caatcaaacc accaatgatc aaaggtgttg ctaattgaaa
     1381 aataactgct aactgtgcga tcgttaccgc aaagggatac gcactaaaat attttaaaat
     1441 agcacctaat actgcattag gaatcaatcc aataatgatt cctgttgctg taccagcgag
     1501 gactttattc agaaaaattt tcggtgatat tttttgtgaa gtttccattc aatctctcct
     1561 ttattttacg ttcaaactat cttcttttgc atggacaagc tcagtcaaga aatcacaata
     1621 aggcgtcgca acaccatatt tcttcccttt tctagaaatt gcgccattga tataatcgat
     1681 ttctgttaat cgatggttct taatcaagtc ttgatacatt gaaggataat gtaacccaat
     1741 tgtttccggg tcaaaacaag attcacaatg tgcaatgact tctgggacat ctagttcaat
     1801 cttctctact gctgctactt tggcaaactc attgacaatc gtcgccacca ttttatgagc
     1861 agtcgatgtt tttcctagct cagccatatt aacgtctaag attgtgcata gtccgttcat
     1921 tgttccgttc acacatgctt tgcgataaat agaatagtga atattgtcag aaaaatgagc
     1981 atttaatcct gattcagaca atttgtctgc taattttttt gcagctgctt cttttccatc
     2041 acctaaattt tgtaattcta ctgaaccact tccaaataat ttgacctgac caggaccttc
     2101 aagacctgcc gtccacatgg tatttccaat atagatattt tccattggta caaatttctc
     2161 aataatatct tcatgcccga taccatttaa tagacataag acctctgtat cttttttgat
     2221 taatgattgg atatcctgca gcattttttc cagctgcatc gctttggtaa atagaataat
     2281 cagatcaact tgatcttctt tttctacttc ggattgaaga acgattggta gttttgcttc
     2341 tacctcttta ccattaaaat ttgcttgtaa tccatgttct ttgatttgtt ggacatgttc
     2401 tgcccagcca tcgattaata atacttcgtt cccactttgg tgaagcatta acccaaagcg
     2461 acttcccatt gcgccagcgc ctgcaattgc tattttcatt gattcttcct cctttttctt
     2521 tctattatac ttatttgaga atcatttcac aaccttgttt tgttattctt ctcttattta
     2581 tcatgtttat atccaccatt caagttattt tatcagcaaa tcaataaaaa ctggaataga
     2641 atcccaattt catcgtaaga gaaactagac tagcaataag aaaatagaaa cattcataga
     2701 aaaaacctca ctcctctgat tataaaagag gggacattaa cctcgtgtat actaaaacct
     2761 tccattcatt cgatggatga ttttcttttt gtgaaaaaat attcattgta gtacgatgtt
     2821 gcgcaacaca gacggatggt attgcttatt atttattaaa atcgaacaat acacttattt
     2881 aaaaacttct ttcccagatt ttcttccatt tttacagtta taactagtat tatcatgaaa
     2941 aatagccttt tattgtctat gggaataata atttaaaatt ggtacttttt tattaattag
     3001 ttcatcgccc aataatctct ccagtttctt agcatctcct gactcaaccg gaattatcca
     3061 agcccagtcc aaataatttt ttaaaatctc atcatatttt gcaaatattt cattgccata
     3121 ttccgtcctt aaaattatat tttcagtgcc agacaagtga tttgtattga gttgataaaa
     3181 aagatctcca tctaaaaaaa tatcattaga agaagatttc cccacagtaa tgaccattcc
     3241 atcagctcta tgtgttacat acaaataatt gtatgacctc acttcaccta atgaatcaat
     3301 tatttcttgc cctttagctc taatatattg atctctatta cctatctcac tcttatttaa
     3361 caaaagtttt ttcatgtcaa atttaatcgg ctgttttatt tttgtaaatt cgcttctact
     3421 actaatccaa tccgagatta atttttcaac atgtcccaga gtaaactctg atttattcgc
     3481 taaatacttt ttttgttgta ttaaagacat ctttccccga agctcaaaat tgagcctaca
     3541 tcgattctct atctcttcaa aaagcatctt atcttcatca ttcttatatt catcattcat
     3601 aaaaccaatc cagctcctcg attaagataa tattttaatc tcatacaaaa actagctctc
     3661 atcaaaacaa ccattgtttt gatttctcat gaaatttctt tatacttttc tccgattata
     3721 aattcctaac cccttgagaa aattacgcat atatttatct ccgcaaggct taaaatttcg
     3781 ttggccttct tttctcaaaa atgccccgat ttcgcctttt gatggataga gttctgcctc
     3841 agcaaaaact tctaaaatat catcagtggt catagacaag gcgattttta atttttttag
     3901 caacacattg ttgatatatt tgatttcctt gcttttttgc ggcgtgtcta ctatcggctt
     3961 atctttttgc ggaccacgcg ccaagagaat caggccattt aggaatgcgt caaaaacttg
     4021 gttgttgatg gttttttcat acacattatt ttctgcatta tctttgttct tatcctgtac
     4081 tttggtcagt accgcctgag cttcttcttt agtcagtggt acaccgccta gctcaaaagc
     4141 tttaatcaaa tccgtatctt tcaaatcaat cgcataacgc aatcgcaata acctatcatt
     4201 gttattcatt tgttcctccg aagtatttag tctttacttt cttacgtact ttttgtcttg
     4261 ctaagatttt taggatcaat gataaaaacc gcatcactta tgatacggag ctgggtatca
     4321 tgagtgaatg atatttataa atcactggat attgaagtat attctgtttt ctcaacttat
     4381 ttagtcagat tttttaatga agattttttg agtaaaattt ctataattcc tacttattga
     4441 aaagtagttt gtagtgctgc ttctacctct ctcatctgtt ctacaaattc caaataactt
     4501 gacgcagctt gcggaatgaa aaaatgatat gtgaatattt ctcctcctag cccgcttaat
     4561 atgcctattt tttataccca tgatttgcag gaaggggatc ggaagtaggt tgcagaaagc
     4621 cgcctttcat aatctgtcgg aaaccggaca tcaaaacgga ttattttact tgtcacattt
     4681 gtattttctt gtgaggaagt gccaaataca ttatttactt gagtgacatt ctcattgtcc
     4741 tgtctgatat tgcttcgtat aaaagtacca gatccacgtc tcgtatataa caaaccttca
     4801 tcaataagga gttgtatagc tttgcggata gtaacacggc ttgttttaaa ctctctagcc
     4861 agactttctt gctcaggtaa tttagaacct tcctgatatt ttccctcaac gatcctctct
     4921 tttagagcca aggctatttc tttatatttt ggcatatttt cccctcattt cattgatcct
     4981 acaaaaaaat tagtatatat aca
//
LOCUS       DAZUMV010000157         4997 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000157 DAZUMV010000000
VERSION     DAZUMV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.161"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ACG8FK_002581"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ACG8FK_002581"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS5098132.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ACG8FK_002582"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ACG8FK_002582"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS5098133.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ACG8FK_002583"
     CDS             2594..3379
                     /locus_tag="ACG8FK_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5098134.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ACG8FK_002584"
     CDS             3392..4243
                     /locus_tag="ACG8FK_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5098135.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ACG8FK_002585"
     CDS             complement(4367..4819)
                     /locus_tag="ACG8FK_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098136.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1690 a          817 c         1040 g         1450 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaat atacctg
//
LOCUS       DAZUMV010000158         4994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000158 DAZUMV010000000
VERSION     DAZUMV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.162"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(329..2056)
                     /locus_tag="ACG8FK_002586"
     CDS             complement(329..2056)
                     /locus_tag="ACG8FK_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS5098137.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2049..3242)
                     /locus_tag="ACG8FK_002587"
     CDS             complement(2049..3242)
                     /locus_tag="ACG8FK_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5098138.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3429..4073)
                     /locus_tag="ACG8FK_002588"
     CDS             complement(3429..4073)
                     /locus_tag="ACG8FK_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5098139.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4167..4301)
                     /locus_tag="ACG8FK_002589"
     CDS             complement(4167..4301)
                     /locus_tag="ACG8FK_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS5098140.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4303..>4994)
                     /locus_tag="ACG8FK_002590"
     CDS             complement(4303..>4994)
                     /locus_tag="ACG8FK_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFS5098141.1"
                     /translation="PFIMELPAYHFPQWSAVLRQTYDRSKSFVKKAGTIIFVSSIVIW
                     FSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGTVAAITGLVAKENIIGTF
                     GILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAPCFAAIGAIHREMNAKKW
                     TWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILLMIGLVYVLFRQPKSKNQ
                     FYTILSLEGEE"
BASE COUNT         1399 a         1108 c          869 g         1618 t
ORIGIN      
        1 caattccttc ttgaaacgta gcaaaataat tcctgaataa gaatttaatc acttttaagt
       61 atataaacta tgtatattct ttaatatttt ttgagaatgc acttcgtaca taataaactt
      121 taatcacatt agtctttcat acctacgatc gatctctttg actcgcaccc ataaattcat
      181 aatagtttgt cggcgtcttt gaacgtttcg aagatgagct acgcattctc tttttttata
      241 gacaaaaaag atagagacga acatggagct tccaaatttg gatatttgat ccaattttgg
      301 aagctccatt tatttcgtgt ctgtcttttt attttttcca gctttcgact aattcttggt
      361 tctctttgat ccagtctttt gctgcttgct gtggatcttt tccattgttg atttccaaca
      421 taactttttc catatctttt tctgaccaat ggaaattgtc taatactttg taagcttctg
      481 gctggtcttc tttcaatcct tttcgaacca tcgtatggat ctgctcttcg cttcccattc
      541 cgttttcagg atcttctaag tatttcagat catatttggc aaacatccaa tgaggtgtcc
      601 atcctgtaac tacaattggt tcgtgttttt tgatcgcttg acctagtgca acggtcattg
      661 ctcctgaaga agatgtctct actttccagt ctttcaagtt gtcatatttt tggatcgtgt
      721 tttcagcagc cgtaactaca ccagctcctg gctcgattcc tatgattttc ttacctgcct
      781 gatcggttaa ctcatcgatc gaatttacat ccatgtaggc tggcactgct aaaccaactt
      841 ttgctcctgt caaattggca cctaggtctt cgacttgatc tttatattgt gcatattgtg
      901 aaccatgagt tttaggtagc caagctgaaa ccatggcatc tgactcgcca tttgaaacag
      961 atttccacat gattgaatta tctaatggcg tcatcgttac atcatagccc atttgtttca
     1021 atacttcacc gactacattt gttgaagcaa cttctgtatc ccattcaaca taagataagt
     1081 tgatacgttt tgtttctttg gcagatgaaa aactccctat accaatcgca cctaagatca
     1141 ctactactac agcaccaatg atcagtcctt gacgtttttt acttttctgt gttgttgctc
     1201 ctgcagcttt tttattgttt ggctggttca aatgctgtgt aaaacggtca atgatgattg
     1261 ctaagataac taatgcaacc ccgttgacaa atccattccc gacttgtgct ctttgcaatg
     1321 ctgataatac gccgcgtcct aagccgggtg caccgatcat agaggcaata acgaccattg
     1381 aaagtgacag cattgttgtt tgattgatcc ctgccatgat cgtactttta gctaaaggca
     1441 attctagttt gaatagtttt tgacgacttg tgctcccgaa tgaatcagaa gcttctacta
     1501 attctttagg aacttgtcga atccctaaat tcgtaaaacg aacggttggt ggcagagcaa
     1561 agatgactga ggcaaatacc cctggaacca tcccgatacc gaaaaatgca actgctggaa
     1621 tcaaataaac gaatcctggc attgtctgca taaagtcaag gatcggtttg ataatacttt
     1681 gggctttact gctttttgcc atcaagatcc ctaatggtac cccaatgatg atcgagatca
     1741 cactagataa aagaacaagt gtgacagtat tcatcaaatc attccataga ccttggttat
     1801 aaataaacca tagaccgatt aatgtgaaca acgtaagtcc tggacgtttc ttagaaataa
     1861 agaaagctgc taccgtcaat aacaagataa acaaaggagc tggaatcact aacagtaaat
     1921 ttgtaatccc cgacatcact gcctgtccaa tcgtttgtaa aaatgaaaat aggccagaaa
     1981 aagttgtcgt aaaccattct gtgatatttt ctacccagtt tgccactggt aattgatagt
     2041 tcaatagatt attcatgttc actcacctcg cttgtttctg ctaatgcttc aatcacacta
     2101 cctcggatga cgacaccttt taattttcca tcctctacaa ctgcaagtgg agtcggtgag
     2161 tcataaataa tattgaaaat atcacttacc accatgtctt tcccaatcac cgtcacattt
     2221 tcatctagga agtcgatcaa tggacgtttt tgcttcctag cttctaacgc ttgatctgca
     2281 gtgatgatcc ctttcaggtg tctctttctg tcaacagcca tcagcatgct gacttcttca
     2341 ttacgcatcc ttgtcaatgc aacgtttggt ccatcagatt cgatattcgt cgtcaatgca
     2401 ggtaccatga tgttttgagc cgtaagcact ttggaacgat cgacttcttc cacaaattca
     2461 cgtacataat catttgctgg attggtcaat atttcttcac ctgttccgat ctgcatgatt
     2521 tcgccgtctt tcatcaaggc gattctgtcc ccaatccgta aagcttcatt caaatcatgg
     2581 gtaatgaaaa taatcgtttt ttgaacattg gcttgcaggt ccagtaattc gtcttgcatt
     2641 tctcgccgga tcagcggatc aagagccgag aacgcttcgt ccatcagcaa gatctcagga
     2701 tcatttgcta aagctctggc taggccaacc cgttgttgca tcccaccaga taactgacta
     2761 ggatattggt ctttaaatga aagcaaacta gaattttcta atgctttttc tgctttttct
     2821 tgtcgttctt ctttaggtac accacggact tctaacccgt attctgtatt ttccaagatc
     2881 gttcgatgtg gaaacagacc gaagttttgg aaaaccatat tgattttatg acgacggact
     2941 tctcgcaatt cttctttgct taacttcgat acatcttctc catcaatata gatactgccc
     3001 gaagttggct caatcagacg attcaataga cggatcaatg tggatttccc actcccggaa
     3061 agccccatga tcacgaagat ttctccttct tttacgtcaa aattcacgtc atacacacca
     3121 accgttgcac cggttttctt taaaatctcc gttttcggct ggtgatcatt catcatatct
     3181 aaagcggctt gtgtcttttt accaaatatc tttgttaaat gttttacttc tacttttacc
     3241 aaacaaattc tcctttttta tgtaaattca tttttctttc tgaaaaaagt gaccacacta
     3301 tgttaacatc atgtagtcac tttgtcaaaa atgaaagaaa tttgcttgtc caaactgaaa
     3361 aaaacttggc taaatctttg taaataaaag gtttagccaa gttaatattt ttttaatatt
     3421 tcacacaatc attctgcaag ctgatgctcc gggtaaaaaa agtttgtcat acgctgtagt
     3481 gtatattcat ttccaataaa atgaacgatg tcccctgccg taaatttagc ataggggcca
     3541 ggtgaaacaa gcagttcttc attatgagaa attgcgataa ctgtggcgcc agtcgcctgc
     3601 cagatattca actcgctaat tgaacggtcc agatgcattg ctttttctga taaagataat
     3661 tcaaacggaa gtaaaggatt catcttatgt attcgcttcg tttgattgac cagctgatcc
     3721 aataattgag aaaaatgatt cagctcatct tgttgcttct ccacacttgc catgatttct
     3781 gcttttgtat cttggatgga ttgtacatct tgatattgca ataggaaatc ttttgctttt
     3841 tccttcgaag caacaaatgc cccacttcca tgccgtactt ccatgattcc aagatctacc
     3901 agcacattga tagccttgcg ggtagtctca ggagaagcat tgtacgtact ggcaagtgtt
     3961 gagcgggcat ggatcttctc acccacttta tatcggtttt caacgatacg ttcggctaaa
     4021 tcaacagcaa tctgctggta gcgcgggcgg acgatttttt tctttccaga cattttcttt
     4081 cctctttctt tttttacttt ccctcatctt actatgctct agcaaaataa gcaaaaaagg
     4141 ggagaatagc ctcttgcaac gactgatcat cgcttgatct gttccttttt tactgggcaa
     4201 gctgtttgac agtcatcaca attcttaccg gattttaaac gagtatagac aattgcgcct
     4261 gcacttccaa agattaacac acttaataag aatgtcgcca tcctattcct ctccttctaa
     4321 tgacaatata gtataaaact ggttcttgga ttttggctga cggaacaaaa cataaacgag
     4381 tcctatcatc agtaaaatag caagatacgt ccctaacgct acctgtccac cctccaataa
     4441 gtgtcccaac tgataaatca caaaactaac taaataagct aaaccgcatt gatagcctat
     4501 ggcgatccaa gtccatttct tggcattcat ttcccgatgg atagccccaa ttgccgcaaa
     4561 acaaggcgca caaagtaaat tgaaggctaa caatgaataa gctgctgcgg gagtatatgt
     4621 gtgttgtagc acttgccaga tttctctgcc attttcggat acttccgcat gaccaaacaa
     4681 aataccaaat gtgccaataa tattttcttt agcgaccaat cctgtaatcg ctgcgactgt
     4741 tccttgccag tttccccaac caagcggctg aaaaacaact gccaggattc gcccgaaaaa
     4801 ggctaagata ctttggtctt cagaaacagg ttgagcataa aaattataag aagagctgaa
     4861 ccagatcacg atactagaaa caaagataat cgttcctgct tttttaacaa aagacttgct
     4921 gcggtcatag gtttgtctta ggaccgctga ccattgagga aaatgatatg cgggcaattc
     4981 catgataaaa ggag
//
LOCUS       DAZUMV010000159         4974 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000159 DAZUMV010000000
VERSION     DAZUMV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.163"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..741
                     /locus_tag="ACG8FK_002591"
     CDS             55..741
                     /locus_tag="ACG8FK_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS5098142.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            743..1687
                     /locus_tag="ACG8FK_002592"
     CDS             743..1687
                     /locus_tag="ACG8FK_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS5098143.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1886..2530
                     /gene="pcp"
                     /locus_tag="ACG8FK_002593"
     CDS             1886..2530
                     /gene="pcp"
                     /locus_tag="ACG8FK_002593"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS5098144.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2609..3886
                     /locus_tag="ACG8FK_002594"
     CDS             2609..3886
                     /locus_tag="ACG8FK_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098145.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQQFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4070..4819)
                     /gene="pnuC"
                     /locus_tag="ACG8FK_002595"
     CDS             complement(4070..4819)
                     /gene="pnuC"
                     /locus_tag="ACG8FK_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS5098146.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1602 a          870 c         1060 g         1442 t
ORIGIN      
        1 tatgtgtttt gctttataat taaaaaaata ttgtcatttt atggaggaaa atatatggaa
       61 aattatctta gccttttagg cgtcttgatt gttattctcg ggtttgcatt taaattagac
      121 tcgatattga tcgtaatggt tgctttagtt gttacagctt tgactggcgg tcttggcatt
      181 caaggaatgt tagaactttt aggtacaagt tttgtaaaca atcgcgggat ggcgattttt
      241 atcatcatta tgttggcaac aggaacgctt gaaagaaatg gtctgaaaga atcagcggct
      301 tccttgatca agcgattcaa gaaagtatct gctggtatga ttgtagatat ttatgggatc
      361 tttcgaatga tctttgcggc ttttaacgtg agttttggtg gagttgctgg atttgttcgt
      421 ccaatcattt taccgatggc attggggacc atcgaatcaa aagggttaga agtccatcct
      481 aagcatgaag aagaactaaa aggtatggct tcagctatgg aaaatgtctg ctggttcttt
      541 ggccaagtac tattcatcgg aggagcaggg gcgttgctag tccaatcaac actgaaagat
      601 ctaggatatg acgtaacatt gggacagctt gctttagtgg aagttccagt ggctatcacg
      661 gctttagttg tggcaagtgt ctacttctat cttaaagaaa agaaattagc gaagaaatat
      721 tatccaggaa aggggcaata agatggactt ttttacaaat cctgatattt tattaggcga
      781 taaattactg gagatccttt atatcgtgat gggactcgta ttgatttaca ctggagtaag
      841 aaatttaaca gataaaacca atcctcatcg ttatgggtca gcctttttct ggacggtctt
      901 aggggtagtt attgcaggag gaagatggct tccggcaatc gtgaacggtg gcttgatctt
      961 tgcgatgacc ataccagcaa ttgctaagaa agtcagcaaa ggagaaagcc ggcttccatc
     1021 aaaagaatat atggaaaaaa tgtcagacaa actaggaatg aaaattttta ttccagcttt
     1081 gagtatcggt gtctttgcga tcttgtttgc tctatttacc aatttaggtg ctttagtcgg
     1141 cgtgggcgtc ggtgtttttg cagccatgct aatcttgatg ttcttttctc gagataatcg
     1201 accaagtaca tttttagatg atgcagcaga tatgctgggc acagtagggc cgttaagcat
     1261 gttgccaatg cttttagctt ctctaggtgc ggtatttaca agcgctggag ttgggacagt
     1321 gatttccaat gcagtaggta cgatcgttcc agaaggaaat gtgaacatcg gcatcatcat
     1381 ttacgctctt ggcatggtga tatttacggt aatcatgggg aatgcatttg cggccatcac
     1441 cgtgatgacg gttgggatcg gtgcaccatt cgtatttagt catggagcga atccagcgtt
     1501 aataggaatg gttgcattga cttgcggttt ttgtgggacc ctgctgacac cgatggctgc
     1561 taacttcaat atcgtgcctg tagcgatgct ggagatgaaa gacaaatacg gagtgatcaa
     1621 aaaccagttg tttattgcgt tatttatgct agttttccaa attgcttata tgattctatt
     1681 taaataatag aagagagggg ttgtgacaga ataattgtcg caacctcttc taacattgta
     1741 gtggaacact atttttttat aagaatatat taagtttgat ctgttatgtt atttaaacta
     1801 ttgtttcctt tatttacgct gttcaaggaa aatggtatga taaaagataa ccatatgtta
     1861 aatcaatcgg acggaggaac aacagatgaa agtactagta acaggttttg atccatttgg
     1921 tggggacaaa gtgaatccag cctatgaagc agtaaaaaaa atgccagatg aaatcagcgg
     1981 tgctgaaatc atcaaagtgg aagtaccgac cgtattcgaa aaaagcagtc aagtagtcaa
     2041 agaagccatc caacagcacc aaccagatgt cgtgatctgt gtcggacaag caggtgggcg
     2101 gtcagcagtc tcgttcgaac gtgtcgcaat caatctagca gaagcaagaa tccctgataa
     2161 tgaaggaaat cagccattcg atacggcttt agaagaaaac ggtccagccg cttactttac
     2221 ctcattgcca atcaaagcaa tgacaaaaaa tgtacaagat catggtctac cagcgtacat
     2281 ctcttataca gcaggaacat ttgtctgcaa tgatatcatg tatcgtttgc ttcatttgat
     2341 cgaaacacaa ttcccggcaa ttcgaggtgg gttcatccac gtgccattct cacccgatca
     2401 agtgatcgat cgtccagtgg gtacgccttc tatgtcttta gaagacattg cttcttcttt
     2461 gacttatgca gttgaagcag cagtagaaaa taaagaagat atttccggaa atgccggaac
     2521 aactcactaa tttagcaaga aacactggct ggttgctaac gttatgttag tagccagctt
     2581 tttgtaatta gaaaggaaag aaacagccgt ggaagaatgg caatccgtct ttgaagaatg
     2641 gtttccaaaa gaaatcagca aaagctatcc catcaaaatt tctaaacaat acacaagcag
     2701 tcaacgttgg gagatctatg caaaattaac gaaaaaacag cgggaactag tcgataagca
     2761 ccgccgatat ctaatcagct ctcgttttat ggaagaacat tatttagctg caacagattg
     2821 ggtgttttct gacttcaaga tcaatccgtt ttttcgaaca aaacgaagcc agcaaaaatt
     2881 gtattgtgag tgtggtagag aattaaaagt gcaatacatc gttaaatcgc caaaaaccgg
     2941 aaagatccta aaactaggca tcaaccactt tgccgatcac ctgcatgtct ctccgacagt
     3001 ggcagcttcg atccatcagg gaatgacaaa agttgatctg gccttagacg aattattatg
     3061 gcttaagcag aagaatatcg attttcctga aggactatgg caaaaatact gttttgtctt
     3121 gtaccaaaat cgtcggatga aacagccata tttaccggat ataaaattag ctcagcggct
     3181 cgcagaattt cgccaagtag aaatgccgat ttacatagcg gattatcaag cattggaaaa
     3241 tgaaatcaag aaaataagtg aacacatcaa tgggcagtca aaaaagcgac agataaaaaa
     3301 agaactattt gacgactttg cagaagaatt agtaaaggat gtagaagaat tcttgatcaa
     3361 ctatcgcgca tttttacgta aagattggca atccatcgta tatgaagaag tgccagtaca
     3421 tcctaatgct tattttgaaa catttatttc cgtgttgaga aagacaaaac gtcaacgaac
     3481 accagaagtc actgctcaga tggaatattt tgcgaaaaac caacaattca tccaaccaaa
     3541 aatctatcta tttatttgga agcaatattg tcgctatggt tttacagaag ggttcttcga
     3601 cagcattcct cggatagtca gaaatggttt tttaaaagta ttgcgtaaag aacgagaagc
     3661 tatacaatct gctgataaga aagaccgtac agtctcaaaa gaaaaatggc aactagtagt
     3721 gaaagatatc caatctggaa atgtgcaaga gacaatcgac aaatggaaag gaaaacatta
     3781 tcgttttact gaagctcaga aacaagcgtt ggaatattat cagaagttag aagaatcact
     3841 gcgtttcaat gatgaggcaa gaaagtatct gaaggaatta ctataaaaaa tagaggctgg
     3901 gacagaagcg tttaactcca agaaataaga agaaattcac gaaaattgct tttcaaattt
     3961 ttgtgaattt cagcttattt ccgaaggagt tgcttctgat tccgccgttt atacgtgttt
     4021 agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tcagtttttt tatgcgtgtt
     4081 gttgtgcgtt gtagtattct tcttccattt tttgcatacg ttcacgttct ttttggttat
     4141 accaaggtga aacggtaaat gcaaggacat cattcagcaa gtaaacagag ctgttcacga
     4201 acatcgccaa acttgcagat ccatgcgtaa atgaaatata ccaaaggacc atttgagcaa
     4261 gccctgaagc taaccaccag taatattggt tgttgtaacg caagaaactg atgataccag
     4321 cagtcaagct aatagaaaaa gcaatggcat ctacccacgg ccgaggatca tctgtataac
     4381 gaccaatcag gaatcctgaa acaagataga caaggaaagt actgatgatt gcgataaacc
     4441 agctttttcc agtgaatttt cggatttttg aagccatatt atgattccat tctttactta
     4501 acaatacggg gatatccaat gtgatcatat aagcaatctg ttcaccgata cttaaatagt
     4561 tctttgctga aaacccaacg ataataaaac aaatcgcaga caaaatacct aaaataccgt
     4621 tgactgattt tgccgcattg atggataaaa tacacaatac accaagcgtg gtaccgataa
     4681 atgtaataac ggacagccaa gtgatgggac tattgacaag agtcatgacc tggcagccaa
     4741 gactgaaaaa gaaaagataa taattttgtt gcggccagcc ttttagctga tgagctaaaa
     4801 actgaaaata tgacttcatg tatgaggact ccttaagaca ctaaatgtag tgtgttctgt
     4861 atttactaac acaatctatt gtatgctttt cttgaaaaaa aactagtctt tcaattataa
     4921 ataatttgtg ttatgataat tttctgtttg ttttcttgaa aatataattt ctta
//
LOCUS       DAZUMV010000160         4899 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000160 DAZUMV010000000
VERSION     DAZUMV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.164"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8FK_002596"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8FK_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS5098147.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACG8FK_002597"
     CDS             complement(2064..3062)
                     /locus_tag="ACG8FK_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS5098148.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACG8FK_002598"
     CDS             complement(3288..3941)
                     /locus_tag="ACG8FK_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS5098149.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8FK_002599"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8FK_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS5098150.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1448 a         1020 c          753 g         1678 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt aatatacagt aaaaccagca ttctcgcttt ttttatcatt
     4861 taaaaattag agttttcaaa tctaattcaa attttctaa
//
LOCUS       DAZUMV010000161         4871 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000161 DAZUMV010000000
VERSION     DAZUMV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.165"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8FK_002600"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG8FK_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS5098151.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG8FK_002601"
     CDS             724..1878
                     /locus_tag="ACG8FK_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS5098152.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG8FK_002602"
     CDS             2093..3061
                     /locus_tag="ACG8FK_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS5098153.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8FK_002603"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG8FK_002603"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS5098154.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG8FK_002604"
     CDS             4091..4699
                     /locus_tag="ACG8FK_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFS5098155.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(4779..>4871)
                     /locus_tag="ACG8FK_002605"
     CDS             complement(4779..>4871)
                     /locus_tag="ACG8FK_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5098156.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         1501 a          936 c         1135 g         1299 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaaatc ccttacggta tctatgattt aagctgggat tcccaataat
     4801 accttgattt cagtacagac cgaaaacccg aagagagtgc cttcttttcg ggttttctta
     4861 tataatcctc g
//
LOCUS       DAZUMV010000162         4852 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000162 DAZUMV010000000
VERSION     DAZUMV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.166"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACG8FK_002606"
     CDS             complement(220..972)
                     /locus_tag="ACG8FK_002606"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS5098157.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACG8FK_002607"
     CDS             1085..2005
                     /locus_tag="ACG8FK_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5098158.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACG8FK_002608"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACG8FK_002608"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS5098159.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="ACG8FK_002609"
     CDS             3311..4789
                     /locus_tag="ACG8FK_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098160.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1703 a          779 c          935 g         1435 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aa
//
LOCUS       DAZUMV010000163         4834 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000163 DAZUMV010000000
VERSION     DAZUMV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.167"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..1670)
                     /locus_tag="ACG8FK_002610"
     CDS             complement(60..1670)
                     /locus_tag="ACG8FK_002610"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS5098161.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1892..2137)
                     /locus_tag="ACG8FK_002611"
     CDS             complement(1892..2137)
                     /locus_tag="ACG8FK_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098162.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2863..4203)
                     /gene="glnA"
                     /locus_tag="ACG8FK_002612"
     CDS             complement(2863..4203)
                     /gene="glnA"
                     /locus_tag="ACG8FK_002612"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS5098163.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4231..4623)
                     /locus_tag="ACG8FK_002613"
     CDS             complement(4231..4623)
                     /locus_tag="ACG8FK_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5098164.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1416 a         1044 c          792 g         1582 t
ORIGIN      
        1 acaagaaaat tgcttatccc cttctcttgc tacttttctc tttctatcaa gttcaaaaat
       61 tattttgttt cgtgatctgc taatgatgct ccgacaaatg ctttgattaa tttttgcggg
      121 cgatttggac gagaaatcaa ttctgggtgg aattggcatc ccacaaagaa ttttttctct
      181 ggcagctcta cgatttctac taaacggtta tctggtgaga cacctgaaaa gactagccct
      241 tgttcttcaa acaattgacg atatttgtta ttgaactcat agcggtgacg gtgacgttct
      301 tgcacaacat cggctccatc atatgcttca gccgtcttcg ttcctttttt gattttacat
      361 gggtataatc caaggcgtaa agttccaccc aaattctcga tgttttcctg atcagccatt
      421 aaatcaataa tattgtttgc tgtatctgga ttcgtttcag ctgatgacgc atcttctaat
      481 ccaacgacgt tacgtgcaaa ttcgacacaa gccatctgca taccaagaca aatacctaaa
      541 aacggaacgt cattttcgcg agcataacga atcgcttcga ttttcccttc tactccgcga
      601 tcaccaaatc caccaggaac caagattcca tctgcgtctt tcaaacgttc ttctacattt
      661 tctctcgtta attcttgtgc tttgatccaa tcgatctcga tgtctgaatt ataggcgaaa
      721 ccagaatgtt tcaatgcttc aactactgag atatatgcat ctggtaactc gacatattta
      781 ccgacaagcg caatccgtac ttttttcttc aagttcaaga cacgttcttc taattctttc
      841 cattctgtca tgtctgcttc tggtgtatct agttttaaat ggtcgcaaac aatttgatcc
      901 attccttgtg cttgtaaaac taatgggatg gagtaaagtg tatctacatc tggcgactcg
      961 ataactgctt ctggttcgac atcacaaaat tgtgccaatt tgtttttcac gctttgagaa
     1021 acaggttgct ctgtacgtac caccaaaata ttcggttgga tacctaaacc acgtagctct
     1081 tttacactgt gctgcgttgg tttagttttc atttctccag cagctttcaa gtaaggaatc
     1141 aatgttgtat ggatatacat aacattatct gtaccgacat ctgctttcat ttgacgaagt
     1201 gcttcaagga atggcaaaga ttctatatcc cctactgttc cccctacttc tgtaatgatc
     1261 acatctgaat cagtcatttt agctgctcgc atgatttttt ctttgatctc atttgtaata
     1321 tgaggaataa cttgaacagt tgctcccaag tattcgccct tacgttcttt acgcaaaact
     1381 tcagaataga tttttcctgt cgtcacgttg gagtatttgt tcaaattgat gtcaataaaa
     1441 cgttcgtagt ggcccagatc caaatctgtt tcagcaccat catctgttac gaatacttct
     1501 ccgtgctgat atgggctcat cgtccccgga tccacgttga tgtatggatc aaatttttgg
     1561 atagttactt ttaagccgcg gtttttcaat aagcgtccta aagaagctgc aacaatccct
     1621 tttccgattg atgaaacgac accgccggtc acaaaaatat attttgtcat aagttcaaaa
     1681 actccttttt aatttatgtg caggaaaagt ctcagacaat aaacaaagct ccctattctt
     1741 caataagaat agggagccga aatttctgta aacttctgac cctctaaaag ggtgcccaag
     1801 gtgtataata caagctgact aagggaaagt caagtactca aatgaatact atccgcttac
     1861 aattttttct ttttcgatat cctttgagaa attatttctt aagtccttta tgagtgcaaa
     1921 aaaaatttaa cccacataat aagcttgata taaacatagc taacgttaaa cctaagcaaa
     1981 tcagaaaatc gttactgaaa aattttaagt gaaataatag ataaaaaaat aaaacaaaca
     2041 ttatgataga taatatccca cctaacaaag aactactttt tcctacttgc agcaacagtt
     2101 tatatagctt atcgtagaaa caatcttttt ctttcaccct tatttacctc ctttacgtaa
     2161 aaaacgtagt ccacagttct cggatttttg gcatatcttt tctattaaat aatatcaaaa
     2221 acaaaacaat tccactaatt ttttcaaaga tgaccttttt ttcaacaaaa gtgccttgta
     2281 gggattcatt gaaggtaacg aatcacataa gtaaaaaata aggagatgcg acaaaagtcg
     2341 tgcctcctta tttctaaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
     2401 tatccatcaa acatgagaag ctgtttttga aattatttct tgtgattcgg agttaaatgg
     2461 taggctcaca acctctggat aaacggtatt caaaagccag tttctcggta ttaagccaaa
     2521 tttatgcaaa aatgtagaaa gccattttca caaaattttt cttaatactc gaaactaaac
     2581 gtctttttca aaacctcttg gtaaacggtg ttcaaaagct tgcatctgcg gtaataagtt
     2641 cagccaattc aaaaacaaga aaagctgttt ttgcttggct attcttatta cttgttgcag
     2701 ggcagctttt tcacaacctc tccactaaac ggtgttcaat cagctgaccc tcggtattag
     2761 ctcaaaaaac ggcaaaatat gaggaactat ttttccgtta gtttctctaa tactcaggtc
     2821 aaaacgctga tttcacagcc tctaattcac ggtattcctg ttttaataaa gttctagata
     2881 ttgttctctt tcccactcgg aaacagtttg tctgaatgcc gcccattcga gacgttttgc
     2941 ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca atcattacat tatccttacg
     3001 caattctttg atagcattat ggatagttga aggtaagtcc tggatatgtg cagcttctct
     3061 ttcttcctca tccattacat aaatgttacg gtcaacagct gctggcggtg tgatctgatt
     3121 acggataccg tctagtcctg cttgcaacaa tactgccatt gccaagtatg ggtttgcaga
     3181 tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg cgagattctg gtacacgtac
     3241 taatggcgaa cgattacggc cactccatgc gacataaaca ggcgcttcat aacctggaac
     3301 gagacgttta taagaattga ctgttggatt gcaaacggct gtgtaggcac gagcatgttt
     3361 cagcaaacca cctaagaaat gataagctgt ttcacttagt tccaattcac cgtctttatc
     3421 atagaaaaca tttccttcat cattgaacaa agacatattg caatgcatac cagaaccatt
     3481 gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt ccatgcttgc gtgcaatcgt
     3541 tttgacaacg agcttgaagg tttggatatt atcacaagca tctaccacat ctgcatattt
     3601 aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt gaagcttcca cttcaaagcc
     3661 tagactttct aattccaaca cgatgtcacg gcggcaattt tcacctaaat ctgttggtgc
     3721 aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg atcccaccgt cttcgtctaa
     3781 tttaaataag aagaattctg gttcaggtcc taaattgaaa gaggtaaaac caaggtcgcg
     3841 catatctttc aatgctcgtt tcaaattccc gcgaggatcg ccggcaaatg gtgtcccatc
     3901 cggattatag atatcacaaa ttaaacgagc aacttttcca tgagcacttt cccacgggaa
     3961 gatcatccaa gtggagagat ctggatacaa atacatatcg ctttcttcaa ttcgaacaaa
     4021 tccttcaatc gaagaaccgt caaacatcat tttattttct aatactttgt cgatctggct
     4081 cactggaact tctacatttt tgatcgtacc taagatgtct gtgaacatca atcgtaaaaa
     4141 tcgtacgttt tcttcctcaa tgattcgttt gatttcttca cctgttatct gtttctttac
     4201 catggtatcc ctcttccttt tttaatttat tcagtcaaag aattgtcttt gcaatccttg
     4261 cgactgataa tgacctgttt tgcttaaccc accttgcgcc aaaatctcat cacgaaaaat
     4321 ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt gcttcttgtt ttttcgcctg
     4381 tgccatttcg tatacacgtt tgatcccagc catattcaat ccctctgaaa gatagtcttt
     4441 aatctcaagc aacatatcaa tatcattcag cgaatacata cgtcggtttc cttcgcttcg
     4501 ttccggatga actaaatctt gttcttcata atagcgaatt tgccgagcag acaaatcagt
     4561 cagcttcata actgtaccaa ttggaaatac ggacatagat cggcgcaatt ccttttctcc
     4621 catcagacgt cccctttcgt tttataagtt ggctaaagtg taccaattaa aaaaaatatt
     4681 gtcaactgca tatgttaggt tttctaacat gagatctcta ttttttataa aataacaaaa
     4741 aaataagaac acaaaaaagg tatctagttg attttcgtac cagatacctt ttatttttta
     4801 ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DAZUMV010000164         4830 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000164 DAZUMV010000000
VERSION     DAZUMV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.168"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..2410
                     /locus_tag="ACG8FK_002614"
     CDS             8..2410
                     /locus_tag="ACG8FK_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS5098165.1"
                     /translation="MLSFSIILSYLIVDLKLCNRTVLSTQKWLNKKYSNVTGFDKVPE
                     NGRTGWPTIYGLIEGLQVELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQ
                     GAFWCKGINPGGFDGVYTPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFV
                     LVPGGDSKIRKMQQWLNVNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATG
                     SYGNGTTSKTYPVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKF
                     MVLPEYTEIADVVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYL
                     TGTVGIGKDERNKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAID
                     AAKNLNIPLTTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCS
                     QIINNGYASFAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSG
                     RDSGFNELSNDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYS
                     GLSWDIISSPVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQM
                     FLADTINDSVSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQD
                     VDAVNVYTLAKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLE
                     NDETVDILGVGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSK
                     LN"
     gene            2490..2885
                     /locus_tag="ACG8FK_002615"
     CDS             2490..2885
                     /locus_tag="ACG8FK_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098166.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3210..3827
                     /locus_tag="ACG8FK_002616"
     CDS             3210..3827
                     /locus_tag="ACG8FK_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5098167.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            3868..4167
                     /locus_tag="ACG8FK_002617"
     CDS             3868..4167
                     /locus_tag="ACG8FK_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS5098168.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            4201..4368
                     /locus_tag="ACG8FK_002618"
     CDS             4201..4368
                     /locus_tag="ACG8FK_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5098169.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4413..>4830)
                     /locus_tag="ACG8FK_002619"
     CDS             complement(4413..>4830)
                     /locus_tag="ACG8FK_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5098170.1"
                     /translation="LDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDT
                     AMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVF
                     KTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         1646 a          675 c          955 g         1554 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gttttaagta ctcagaaatg gctaaacaag aaatacagta atgttacagg
      121 gtttgataag gttccagaaa atgggcgaac agggtggcct acaatctatg gacttattga
      181 aggactacaa gtagaacttg gtattactaa tctagtagct aattttggac ctaccactga
      241 aaaaatgtac gataatcagg ttactcctaa atggggcaaa aatctaccta aaaatattgt
      301 atttcttatt caaggtgcct tttggtgtaa ggggattaat ccaggtggct tcgatggggt
      361 atatactcca tatttagata ctgctgtaaa agagttgcag actgatgcag ggtttaatgg
      421 aagcgctgta actggagttt tggatgcgaa atgggccaaa gcgttatttg atatgagtgc
      481 ttttgttctt gttcccggcg gagatagtaa aattagaaaa atgcagcaat ggttaaatgt
      541 aaattattta gaatacacgg gaataatgcc ttgtgatggt atttatcaaa gaaattcaaa
      601 tcaagcatta atttttgcat tacaagctga actaggatat tctcccagtg aagcaacagg
      661 gtcttatggt aatgggacaa ctagtaaaac ttatcctgtg tctgaaggta actcgggaaa
      721 ctatgtaaga ataattcaat atggattata tgttaatggt tattttgaag acggtacatt
      781 cgatggtatt ttcactaaat atatgggttt agaagtactg gcattccgaa aatttatggt
      841 attgcctgaa tatactgaaa tagcagatgt tgttgtaata aaaggactat tgtctagcgc
      901 tggagatatt agaagatctg cagatggcgc agatacttct acgcaattaa ctagatctca
      961 aatccaaaca ttggttgata atgatataaa aatagttggt agatatctta ctggtactgt
     1021 tggaattgga aaagatgaac gaaataagta tctaactaca gaggaattga ataatatttt
     1081 ttctaaaaat ttatctgtgt ttcctatcta tcaagatgga ggtgcagcat tagcttattt
     1141 tacttatgat cgtggcttat cagatggtaa gaaggctata gatgcagcaa aaaatctaaa
     1201 tataccatta acaacagtta tttattttgc tgttgatcta gacatgttag gggaagaaat
     1261 tcttgcatat gctggagaat attttaaggg ggtatctgct gtaatgtcat atagcgggta
     1321 tcaaacgggt gtttacggaa caagaaatgt ttgttctcaa ataatcaata acgggtatgc
     1381 ttcgtttgca tttgtttcag atatgtcgac tggttatagt gggaatttag gttttcctat
     1441 gcctagacaa tggtcttttg atcaatttgt tgaatttacc ataggaagtg gcaacggagc
     1501 tgttggtatc gatttaatcg ctacatcggg tagagatagc ggatttaatg agttatcaaa
     1561 tgatactaat aatgataatt acattgccaa atacaatcag aaggttataa atcagatggt
     1621 acgtgcgtat caatatctat cacaagctgg tcttgataat ccttggaacc ctcatttgac
     1681 attttataga tatgtaaatt attctggttt gtcatgggac atcatttctt ctcctgtaac
     1741 agagcatgat agaaaaattt atgatgaata taggaataaa ttaactaata gcgagggcct
     1801 gtataattat tttatagatc ctaatactgg ttcaataata ggtttgcctc acttaattgt
     1861 gacgttacaa tcacagatgt ttctagccga cacaatcaat gatagtgttt ctgactttgc
     1921 aggttggtta ggagatttaa tgacttgctg gggtgaagtt aagaaattag gaatacaaat
     1981 ggagcaaggt gtatttgcat tagtaggtac ggctgcaggt ggaacattta gtctagaaga
     2041 tttataccaa gatgttgacg cggtaaatgt ttatacctta gcaaaaagaa attctgattc
     2101 aacagcttta tcggctttaa aaacgtacta ttgtgataag ggtgctattg atagatttaa
     2161 tcaatttgtt aagaacagat ttggttcttc agataaaatc gcttcagtaa caaatagttt
     2221 attagaaaat gacgaaacag ttgatatctt aggtgtaggg ataatttcat ttttcattga
     2281 taaaatagtt aataattcac ttgggacaga ttttacttat cttgatttta ttggagatga
     2341 ggggaaagtt gcaaatggat tttctgaaaa aattattcaa tttcaagaaa atagaagtaa
     2401 acttaattaa gaatcgttta ctatttaaac agtgtccttt tcctttttaa atatatacga
     2461 taaaataagt atatgaattg taaaaggaca tgatgattat ggaagaaata gaatggaata
     2521 atctgactca aaataataaa aataattctg gatgtggaat tatctttatg tttgttatag
     2581 ttttgggaat agtagtactt ttaataactg tcaagatagt agattacttt gcgaatactc
     2641 ctgaagaaca aatacagaga aaacttcctg agtttgaaag agtaccacct tatgatccaa
     2701 gagataattc atttcaagta aaaaaattaa aagagtctcc aaatgaaatg tggtttgaag
     2761 ttacagttga atcaagcgac ttcaaagaaa aaaagaaaga atacaattat attgtaaagg
     2821 caaaagtatc aaaaagattt atttttaacc ctaaattaga aatatatagt gttgtaatag
     2881 aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca aattctgatg tgaaaaataa
     2941 aatataccat ggaagacata aaagaaaaac tggataacta gtttatttaa tggcagaccg
     3001 ttaaggcctg ccatttttat gtattttagc ggaataaata taatcatgcg ggagtgtaaa
     3061 cttactgttt caaaaagtat actttttgac atgccttgat tttctttgtt tatgtatggc
     3121 ataataacca cataatacaa tttgaaacgt ttctgattta atctgattgt tttgaaacaa
     3181 ttttagtgaa aaaagggtag ataaaaacga tgagaaaatt tttttatatc cgggtcagta
     3241 gttatgatca atcgatcgaa cgacaactga tttccgcgaa ggaattagga atccctgaag
     3301 aatatatatt tgtagaaaaa gccagcggca aagattttaa acgccctgaa tatcaattaa
     3361 tgaaacggat gtttcgaaaa ggagatttac tctatctaca atcattagat agactaggca
     3421 gaaataaaca aatgatttta gaagaatggc gagaattaac taaggaaaag cagattgata
     3481 tttctgtgtt agacatgcct ctgttagata ctgctagtta caagcaacta gatgggttag
     3541 agacgcttat ctctgactta attctgcaat tactcagcta tatggccgaa gacgagcgga
     3601 aacgaattaa tagccggcaa agagaaggcg tagaagctgc gaaacaaaag ggtgtaaaat
     3661 tcggcagaaa gaaaattgct ttacctcctg aatttcctgc gatctattct gactggaaag
     3721 caggaaagat aactgcagtc tatgctatgg atcaattggg catgaaacga aatacctttt
     3781 atcgacgggt gaaagaatat gaacatacct tgcaccactt ttcttgatag atataacagc
     3841 tattagacgg gaagggagag tttgaaaatg gacttaacga atcaaagggt cgtttttact
     3901 ggaacgctgc agcaaatgac acgaacccaa gccatcggtt tggttcactc tttaaacggc
     3961 cattgtcaat caagtgttac ttcaaaaaca aatttcctgg tttgtggtca gtattctaag
     4021 tcgttgtttg atgattctct ctatacaaaa aaggaacaaa ccgcgcggga cttgattgcg
     4081 ttaggtcatg agattaccat tttatcagaa gtcgaatttt atgcgttaat tagtcaacag
     4141 ttgaaagaat ggcaacggta tttataatcg ttagtaaaat ttacaaggag gaaaattttt
     4201 atgaagcgaa aatcttatga tcgttggggc attgaaataa ctagttttaa tcgaaatatt
     4261 gcttttcacg tggagtgtga ctgtggggaa ttagcaaaaa gtgtttatca aaaaaattac
     4321 ttttactgtc ctaaatgcca tcgccgatac ggggtacatg gaacatagaa acgaaatagt
     4381 gcggtagaaa agcgaatgaa tgtagtatat ttctacaaat cattcgcttt ctttatttta
     4441 taaatagttg gtcgagagat cccgttttct tttgcaatct ctgcaactgg aatgccttgc
     4501 tttaacatgt ccaccacggt tttaaaaact aagcgttttt gcggatctgt tgagttaata
     4561 gaatactcga ttggtcgtcc cttatatcgg ccggcttttt gagccaataa tactccttgc
     4621 cgctgacgtt cacgaatttt ctcacgctcg ttttgtgcaa tataacttag cagtgataaa
     4681 aacatatcga acatagcggt atccaggagt tcatttccag tattaaaatc taaaaactgt
     4741 gcatctaata tttttaacgc tacttttttc tgtttcataa aagcaactgt attttttata
     4801 tcttcataat ctcttcctaa acgatctagt
//
LOCUS       DAZUMV010000165         4812 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000165 DAZUMV010000000
VERSION     DAZUMV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.169"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1178)
                     /gene="xerS"
                     /locus_tag="ACG8FK_002620"
     CDS             complement(93..1178)
                     /gene="xerS"
                     /locus_tag="ACG8FK_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS5098171.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1527..1722
                     /locus_tag="ACG8FK_002621"
                     /pseudo
     CDS             1527..1722
                     /locus_tag="ACG8FK_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3254..3772)
                     /locus_tag="ACG8FK_002622"
     CDS             complement(3254..3772)
                     /locus_tag="ACG8FK_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS5098172.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4139..4387
                     /locus_tag="ACG8FK_002623"
     CDS             4139..4387
                     /locus_tag="ACG8FK_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098173.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1582 a          786 c          730 g         1714 t
ORIGIN      
        1 aatataaaaa tgataatgaa gcttgccttt aaagttgggc ttttatctaa ttttagagac
       61 aggcttcatt attatactat tttttactac atttacaaag cgttcaaagc atcaattgta
      121 gctgcattgt ctatatgagt atataaagtt gttgtatttg tacttgtatg tcccaattgt
      181 tgggcagtta ataccaatga atttgtctgt tgatagagtc tagtagccaa agtatgacgt
      241 aatttatgtg gactgacttg gactctgaat ccttttgaat attttgctac catccgttcg
      301 attgcaatac cggatatacg cttggcttct ccttgataaa ttgctagaaa taaagctttt
      361 tcgttttctg ttactttgta tcgttctttc cgaatagcta gataattagc taaatattcc
      421 attgcaaatg ctgctatatt tactgcgtct ctcttatttc cttttctagt tactaccaca
      481 ttattctttt ctaaagatag gtcctctaaa ttaatattta caacctcgga gagtcttaat
      541 ccagatccta aaatcaatgc attgatcgcc aggtctcttt ctacatctcg ttgataatag
      601 atcaaagctc gtttgctcaa agttttttta tattcattgg caatataatc tagataattt
      661 tgtgtatctt cgtctagaaa taattttttc tcgattgcgt ttgcacgggc agctaacgta
      721 gttttatctg tgatagttgc tacttgatcc ataggattat tttctaagta tggttttcct
      781 aaaggattat tggaactttt cgaaagatat ttaaatagaa cctttaatgc tgtgatattt
      841 ctattgatag tagaagtaga tagacctaag ttattattat ctttgagctt agtttttgtt
      901 gtctcttttt ttacattttc ctttgtgcga cgtttcaaat gagatttgta caagtttaga
      961 tcataggcag ttaactgtct tatcgaggtg attgaagtat cgggaacgat agactcactc
     1021 agaagccatt cataaaagag cttataattt tttagatatt ctaaagtagt tcgaggtgat
     1081 tgatctttat cctcacgatc ttgaacaaaa tcttttatat aatcgggcat atgaagtatg
     1141 attgtatcta ttttttgata tatttctgta tttttcatgt tattcatcct ttcgataagt
     1201 aggggagagg agtatctcta taattggact ataccaatta tagagtgttt tgtcaattgg
     1261 tatactctga aatttattaa tttatttcaa aaaagcagat aaataggtat agatttaata
     1321 atttaaaata aatggtatac atgaatagga aaggaaacag aaggaggcaa tcttaatttt
     1381 ttaaattgtc taaattatat agaaagaaca atctttctat ataattagac ataagagaga
     1441 ctacggttga tttgatatta acaagcgtat atgaatgtat atacaaataa aaaaggggag
     1501 ggagaagtta aagaaaattc agagaagtgg ggcactcagt agtattaaca atttaaaagg
     1561 aatttcttaa agaaaatggg ttgcatcctg gagatacaat ttttttagat aaagaaaaaa
     1621 ttcaagcaac aattcaaaaa aatcttaact gctgagattg atgtagcaat atctcaatca
     1681 tcaacaaagt acgatcctgc ctttcaaaag cttattgata aatgtttatt cattataaaa
     1741 ataaaagaaa aatttatgtc agcttttttc atcaaagtgg taatgataat tcgtctatta
     1801 attgtatttt ttaggaaata tggtataatt gttttaacga aaaggtaatt catgatacac
     1861 caaaaaccac tagctggcgg gctagtggtt ttcttttacg ttctattcag attaacgggt
     1921 aggcttactg tcttttttgg caataatttt gcaatcaagc catttacaga tgtaatgcgc
     1981 ggatacttct gctaagattg caaaaaacat atcgaaaagg taattcataa tacaacctcc
     2041 tccccttgcc ataataggcc ggggtagcag acagcataaa tattatacca taactgaaaa
     2101 tttcatttta gtacatttca atatattgat gaaccagcac aataaaacat gccatgacta
     2161 acagaaaaaa acttattaaa ccagatataa cttgtaaaca tccaactata tagaaaacgt
     2221 aaattttcta tatagttgga ttaagataac gactatttat tttatcatat ttttactata
     2281 ccctatactt ttacttggta tatttatgaa taatcgcaaa tttaccccta tactactagt
     2341 ttgtaagtta gattaataaa aatactaaag ttatttgttc ttttattaaa tacacctaca
     2401 acttataaat tgatggtatt tagataacac gaaagaataa aaataacagc tgatagtaaa
     2461 gtttgttgac gattgctact caatcgaaag tgagttagtg cgtatgaact tgcttctgaa
     2521 aacttatgaa atgaaagacc ttttacatct tgcagaaatc gttactggga ttaaactaag
     2581 caataaaaac taatgttccc ctcattcttt gaacttctac tatttgattt atttattctg
     2641 ataaatgacg atttcacctt caggatcttc aaatcccgta tagaatttat ttaagatctc
     2701 ttcagtatca gtaataggat aggcgatagg ccacataccc tttgatggtt agaataaact
     2761 gctcaaaatc tgtatcgtat gttggcttta gcatattaac aaaggctatc tccaaattat
     2821 aaaccataag cgttgaatcg ttcttaacaa tttcaattct tcctaaccca aagtcagaag
     2881 atataattac cggacttgcc ttacccttca tgtgggttgt tgagattcca tatttaaagg
     2941 gaattgtcgg tactatcggc ggtctgaaaa acatcccaaa aataaaaaaa tctttcccct
     3001 gctagtctcc taagtccctt tgtagcggat atggcaccat aatctcattt tatcgaagtg
     3061 agtcatggtt tatctgttgg ccaaccaaaa ttgatacaat ctaacgatgc accctctacg
     3121 gcattctagg ggagtgcact tgcattcacc caataaaaac cggcagttag caaagaactt
     3181 atgtagtcgt atgttcttac tgttaacata caagaggtct cctttccaca attgcttagc
     3241 agtaaacggg aatttacaaa gctaatctct tccattttct tatctttgtt ctaaaagtcc
     3301 caaatggagc aactgtattt acatgaataa atttatatac ttcccataca gccgttttgg
     3361 ttgcctcgtc tgcccatttt cttttgtgtg cagtgaatag atcttcatca ctcattgaat
     3421 caatcatcat gttaatatca tcaatattac gattgagaat ttcctctaat tctttcaatg
     3481 gtagatgtgc ataggtattt gtaaaccact tatataactc tcctaactga ttccatttaa
     3541 attgatcaga aggcgtttta acagataatc catttctttc atcattttcc cattttagaa
     3601 ttaaattcgt ccaaccaact tgatatgcta aattttcagc aggtgttcta tccacttcct
     3661 caactctttt atccttcaat tcttcaggaa tagtttcaaa ttctaaaata tatttagtat
     3721 aagtcctatt tatttctttt tttaattctt ctttattttt atatgttctc attatattac
     3781 ctccataaat tcctaattgt ctcctttaat tcatctataa tttcatccac gatattttaa
     3841 tccgttctac aatgcatatc tgatatgcat tgactgtcta actcatatgt gaatgattta
     3901 taaaatatcc caacgtctca tctaaagaaa tgttgatcgt cagcaaggag ctaaaaaata
     3961 cgaatatatt tcttttgtag taaaatcgga ctggtttgca tatcattttc ttcgtaaata
     4021 tcaaaatcat gttaactact atcaattact aataaaaact accactttaa gcgttaagaa
     4081 ctgttaagtt tagttaagtg aggatagttc acaaaatcca ttgttaaact tcacgaaagt
     4141 ggtaaaactc aaaatgcttt atataggatt acagcatcgc tctttcttcg attgcgcgtt
     4201 gggtaaaaaa tattatgaag taaaaattga cgatgacacg ttccttacgg ctcatcagat
     4261 gaaacagcta caaaaacgaa atgatcaact cgaagaaaat ttaattctaa aaaagcgatt
     4321 gccatattca cgcctcgctc gacgaactat taaaggtcaa gatttacctg aagatcatcc
     4381 tttttaggaa attgaagctt tttatgttgc acaatgcatt tataatacta caatgatgtt
     4441 ttcaccagat attcttctag ggtgcggtgt aatgaaacag atacacttgc aaaagaaaat
     4501 tcaccgcgaa tttactaaaa ttatgaataa ttatgttaat cttccgaaat ttttttccaa
     4561 aattaaggga tcaagctggt gttatagatt gtttaaggtt ggcaaaaaaa taacttcttt
     4621 ttaaacaatt atagaaaacc gatactctgt taagttggtt gacacggtgt cgatttttat
     4681 gactttctta ttctgaaact tcactaacgg acagtaccta aaaagtctat agtatggacg
     4741 tttacgagta ctattttatc aaaaatgctg ctcatttcaa cttatatgtt ttagtgaact
     4801 acccccactt ag
//
LOCUS       DAZUMV010000166         4806 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000166 DAZUMV010000000
VERSION     DAZUMV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.170"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1200)
                     /gene="nagA"
                     /locus_tag="ACG8FK_002624"
     CDS             complement(52..1200)
                     /gene="nagA"
                     /locus_tag="ACG8FK_002624"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS5098174.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1276..2112)
                     /locus_tag="ACG8FK_002625"
     CDS             complement(1276..2112)
                     /locus_tag="ACG8FK_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098175.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2296..3513)
                     /locus_tag="ACG8FK_002626"
     CDS             complement(2296..3513)
                     /locus_tag="ACG8FK_002626"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5098176.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3662..3823)
                     /locus_tag="ACG8FK_002627"
     CDS             complement(3662..3823)
                     /locus_tag="ACG8FK_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098177.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3896..3979
                     /locus_tag="ACG8FK_002628"
     tRNA            3896..3979
                     /locus_tag="ACG8FK_002628"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3930..3932,aa:Leu,seq:gag)
     gene            complement(4219..>4806)
                     /locus_tag="ACG8FK_002629"
     CDS             complement(4219..>4806)
                     /locus_tag="ACG8FK_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098178.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1460 a         1001 c          775 g         1570 t
ORIGIN      
        1 accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat attatgcgtg
       61 gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt tcaatacgat
      121 aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt tacagctgat
      181 tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc cccagtctac
      241 aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc cagattctag
      301 acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat ttccatcagg
      361 catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc ctgctttttc
      421 cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca attcagaaaa
      481 gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat tcaatccacg
      541 cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt tttgcgcttc
      601 ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag tcacttgttt
      661 gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga ttaaaccacc
      721 tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat aacttgggtt
      781 ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc ctttgatttt
      841 agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt ttaaacgttc
      901 tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata acagaccttc
      961 agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca tatagccgtg
     1021 aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa tgatgttgac
     1081 ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa tttcaagata
     1141 gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa aagttctcat
     1201 tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga agaagtcaag
     1261 aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg ctaagccttc
     1321 atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt taagcacaaa
     1381 taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc cttgattcac
     1441 ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat aattataaaa
     1501 aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca gttggatgct
     1561 tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata tttttccgat
     1621 acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt ggctagagta
     1681 gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca acgtgaattc
     1741 gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga
     1801 aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact
     1861 atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac
     1921 atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag
     1981 ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat
     2041 tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat
     2101 taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac
     2161 tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt
     2221 aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa
     2281 atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc
     2341 tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt
     2401 ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac
     2461 gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa
     2521 agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata
     2581 ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc
     2641 gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga
     2701 caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat
     2761 ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac
     2821 gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg
     2881 atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc
     2941 ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat
     3001 agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc
     3061 ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca
     3121 caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat
     3181 acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt
     3241 cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt
     3301 ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt
     3361 tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc
     3421 ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc
     3481 ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa
     3541 gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa
     3601 attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa
     3661 cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa
     3721 tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta
     3781 agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca
     3841 tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga
     3901 tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt
     3961 tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg
     4021 tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta
     4081 caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa
     4141 cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag
     4201 cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag
     4261 cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc
     4321 tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat
     4381 tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat
     4441 catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac
     4501 aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa
     4561 aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat
     4621 cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata
     4681 aataatcagg tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca
     4741 tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga tattcatgtc
     4801 taaaaa
//
LOCUS       DAZUMV010000167         4804 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000167 DAZUMV010000000
VERSION     DAZUMV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.171"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..411
                     /locus_tag="ACG8FK_002630"
     CDS             <1..411
                     /locus_tag="ACG8FK_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098179.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="ACG8FK_002631"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="ACG8FK_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS5098180.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /gene="mobV"
                     /locus_tag="ACG8FK_002632"
     CDS             2446..3708
                     /gene="mobV"
                     /locus_tag="ACG8FK_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFS5098181.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4804
                     /locus_tag="ACG8FK_002633"
     CDS             3953..>4804
                     /locus_tag="ACG8FK_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFS5098182.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1659 a          665 c          979 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaagaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata atgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacagacggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaa
//
LOCUS       DAZUMV010000168         4782 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000168 DAZUMV010000000
VERSION     DAZUMV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.172"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..694
                     /locus_tag="ACG8FK_002634"
     CDS             23..694
                     /locus_tag="ACG8FK_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5098183.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            753..1193
                     /locus_tag="ACG8FK_002635"
     CDS             753..1193
                     /locus_tag="ACG8FK_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098184.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1205..1873
                     /locus_tag="ACG8FK_002636"
     CDS             1205..1873
                     /locus_tag="ACG8FK_002636"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5098185.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1937..2560
                     /locus_tag="ACG8FK_002637"
     CDS             1937..2560
                     /locus_tag="ACG8FK_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS5098186.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2616..3002
                     /locus_tag="ACG8FK_002638"
     CDS             2616..3002
                     /locus_tag="ACG8FK_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS5098187.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3215..4606
                     /locus_tag="ACG8FK_002639"
     CDS             3215..4606
                     /locus_tag="ACG8FK_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS5098188.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1596 a          791 c         1018 g         1377 t
ORIGIN      
        1 tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata ctctgattga
       61 gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc atttttcacc
      121 agagttccat ctagaggggc taacattagg agaaatctat caagcagaag ggctgtcggc
      181 tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata tcgagctttc
      241 tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa tgatcgatac
      301 aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc gttatcaaat
      361 agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa aaagcttttt
      421 aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga aaaaatatcc
      481 aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc atcgttttat
      541 acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca catttcgagt
      601 agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa atcggaatgt
      661 ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg atttttttgt
      721 cataataaat attagatgat ggaggagata atatgatgca aacatggtta gaaaggctaa
      781 tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa tttgatcagt
      841 tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt tggttgccct
      901 atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt cgtttcctat
      961 caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat cttgatagag
     1021 tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt
     1081 atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac
     1141 gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt taagggagaa
     1201 aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg
     1261 gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg
     1321 tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg ttttgaaacg
     1381 tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc ttcctataat
     1441 aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa acaaagataa
     1501 gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat atacggaagt
     1561 tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg atccgcattt
     1621 gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct tatggcaaga
     1681 tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc atggtaatag
     1741 catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg atacgatcga
     1801 tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa ttctggacaa
     1861 aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag aagcaaagga
     1921 atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat tgtttgatac
     1981 gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta tcagtatgga
     2041 caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga tcaatgaatc
     2101 aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag aaagtacgga
     2161 agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag acaacggcgg
     2221 tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt taaaagaaca
     2281 tcgattggca catctgattg aggaagtagt agggatcgat caggactttc ctcgtaaacc
     2341 tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa cagacacgat
     2401 gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag tcgctacttg
     2461 tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg taggaaatct
     2521 gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt caaaggagat
     2581 atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga caaaaagaag
     2641 ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa acagatttga
     2701 aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa catagcatcg
     2761 taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct ggaagtgtga
     2821 gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag
     2881 aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct tatgaggtga
     2941 cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa aaacaagaat
     3001 agacagtaaa aacatacctg cgatagatta aagctatcac aggtatgttt ttttattact
     3061 aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc taaaatataa aagtcactga
     3121 aaaaaatttt ttcaggagga tggttgaaga atcattctcg ggaaccttta cttccttcga
     3181 tggacttgtc attaataata tgaggaggag aaaaatgaca acactcatca cagttttagt
     3241 ggttctagtt tttgcggcat tattatatgt gttctatcgt atgcaaaagc gccatacaaa
     3301 attttcaaca cgcgtcttta cagcattagg agttggtatc gtctttggcg gtctgcttca
     3361 attgatcttt ggtgtagacg acaaagtcgt tactcaatca atggaatgga tcgggattgt
     3421 aggtcaagga tatgtatcat tcttgcaaat gctagttatg cctttagtgt ttatttcgat
     3481 cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta gggaaaatta gtgcgactgt
     3541 attgacttct ttacttggaa caacagctat tgcagcattg atcggaattg gttcaacaat
     3601 gctgttcgga ctccaaggag ctaaattcac acaaggagct gcagaaacag cacgaatctc
     3661 tgaactggca acacgtcagg aaacagttga aaacttatcg attccacagc agattttatc
     3721 ctttatccct aaaaacgttt ttgctgattt agcaggtagt cgtgcaacaa gtacgattgg
     3781 agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta ggcgtgaaaa gaaaagcgcc
     3841 aaaagaagca gcattctttt cagatttagt cgacagtctg tataaaatca ttatgcggat
     3901 cgtgacgctt gttttgcgtt tgacacctta tggagtattt gctttgatga caaacgtttt
     3961 ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa tttgtcgttg cttcttacgt
     4021 agcattgctt gctgttttga ttgtccactc actgattttg attggtgcaa aagtcaatcc
     4081 aatcaactat ttcaaaaaag cctttccggt attgagcttt gcgtttactt cacgttcaag
     4141 tgctggagct ttgccattga atatcgagac tcagacaaaa gctttaggag tagatgatgc
     4201 aacagctaat ttctcagcaa gttttggttt gtctattgga caaaacggct gtgctggcgt
     4261 ttatccagct atgttggcaa cgatcgtcgc accagcagct ggagtgaatg tctttagttt
     4321 ggaatttatt ttgatgcttg tggcaattgt aacaatcagt tcttttggtg tagctggagt
     4381 cggtgggggc gcaacatttg cttcactgat cgttttaggg gcaatgaact tgccagtagc
     4441 aatcgttggg ttagttatct ctgttgaacc attaatcgat atggcacgta cagcagtaaa
     4501 cgtcagcggt agcatggtag ctggtatcgt gacaagtgca cgcatcaaag atttagatcg
     4561 cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat gcatagtcaa aaagaggttg
     4621 tgatagaagt ggtcagcttt aagaaataag aagaagtttc ctaaaaatag tttctcaaat
     4681 ttttgtgaaa tttcggctta tttccgagaa gctacttctg aaacaccgtg aataagaggt
     4741 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aa
//
LOCUS       DAZUMV010000169         4548 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000169 DAZUMV010000000
VERSION     DAZUMV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.174"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(507..848)
                     /locus_tag="ACG8FK_002640"
     CDS             complement(507..848)
                     /locus_tag="ACG8FK_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5098189.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
     gene            complement(838..1119)
                     /locus_tag="ACG8FK_002641"
     CDS             complement(838..1119)
                     /locus_tag="ACG8FK_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS5098190.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            complement(1490..1729)
                     /locus_tag="ACG8FK_002642"
     CDS             complement(1490..1729)
                     /locus_tag="ACG8FK_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098191.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(1749..1997)
                     /locus_tag="ACG8FK_002643"
     CDS             complement(1749..1997)
                     /locus_tag="ACG8FK_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098192.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(2012..2578)
                     /locus_tag="ACG8FK_002644"
     CDS             complement(2012..2578)
                     /locus_tag="ACG8FK_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098193.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            complement(2579..3124)
                     /locus_tag="ACG8FK_002645"
     CDS             complement(2579..3124)
                     /locus_tag="ACG8FK_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098194.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3150..3917)
                     /locus_tag="ACG8FK_002646"
     CDS             complement(3150..3917)
                     /locus_tag="ACG8FK_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFS5098195.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1290 a          954 c          595 g         1709 t
ORIGIN      
        1 cgctacgcga ccgttcgttc gtcttttcct ccgtgggact cacccaccgc aagcggttgg
       61 ttgagttgtg gctcctattc aaaattcact aatctgttat tttttatttc tattcaaata
      121 ggtaacgttc gtttgcaagc aaactctctc tatgtaactt gccagttaca tcagcggtaa
      181 cgctacgtcg caagcgactt gctctaatga aactgccgtt tcattctgcc tgttcgttcg
      241 tagcaagcta cttcactcac cgtatccgtc tgaattattg ttatttattg ctattcaata
      301 tccgtcagac attttattta tttacataca ttttatataa aatatatatt ataattcgta
      361 tattactaaa tatcattatt taacaattct taacgtttta ttaatgttta taaccacgta
      421 tatatttacg tatattttca tatacataaa tacgtttata taaacgtata tcttaatata
      481 catataaaca aaaaagaaaa gccttgttag acttttcttt gtaatttctc gataaaccta
      541 tttagctttt tcgtatcact agctgataac ataccaacct ttttgaatga tatgttctct
      601 cggcttttgg gaatagcctt taaagaccct acgtcaatcc atgattgctt tttaagtcct
      661 gctttttccc attctgcaat aggaaaatac accctcttta tttggtctga ttttttctca
      721 tattgacttg tgattccaaa gaattctact ctatcttctt tatcactaac caccaaaata
      781 ggtcttcttt taccaccatt tttatctgta aatgcaacat ataccgttac tatctcatta
      841 ggcttcatcg aaccactctt ctaacttttc atctgtatct agaacatcta ctggcatgtc
      901 cttagttagc tgttgcaatc tgattccttg cttctcctct tcacttagct ttatctctac
      961 tggaaactca cctctagcga ctacttgttt gtaaagaatt gttattgctg ttgtaggatt
     1021 taaacctagc tcttccaata tcatttctgc gttctctttt aaattcttgt ctattccgac
     1081 ttggattctg cttttttcag ttggattttt gaccttcatt tcatccactc cttttgagta
     1141 taaaacactc attatttatc aaattgtcca catttatgat tatattatac tcttaataaa
     1201 ctacattgca agtaatctaa tcaaattaag atgctatatc atacggtaat tgaacaaatt
     1261 gtcgctaacc cacacatacg gaatcgtaat gaatgaaaaa aagacaagcc tattcgcagt
     1321 aggcttgtct ttgtgcttct cttgtacaaa atcttcacat ttgtaaatta attttagcac
     1381 ctttgttagg aaagaacaac ttaaatgaga tgctaaagaa ttaatagttc tcaatctagc
     1441 atattcactt aataccatct tatcttcatt catggttcac cacctcatct taattatttt
     1501 caaattggca aatcaattta ttttgggtta acaaaactaa tagctgttca ctatcaaaaa
     1561 gaacctcaca aaattctttt tctatcctct cttctggcat gtaataacct atttgtttca
     1621 atatcttatc tggataacct tttcgttggg tttttctcaa taaatcttca tcatttccaa
     1681 cataataata agaacaccat tgcataccct tacttttaaa ttttgtcatg tttctatctt
     1741 cctttcactt attgacccaa ccatgaaaaa atatattttg ctaaatcttc cttttctatt
     1801 ccatataaaa cattctctaa atccaattta tattcctcgt caacaaggct ttctgtatcg
     1861 tcatcataaa gaacatatac tgcataattt ctttcgtaaa aggcattagc aagacttctg
     1921 ctaataccta ccattccttt ccatgtataa ccaaaatcaa ccaaatcttc atgatctaga
     1981 aaatccattc cttccatgct gattcacact cctattctgc tgttgtgtaa tatgcaacgc
     2041 aatcatctgc ccctactaac cacacacacc aatcttcaat ttcatcatcg cttctcttac
     2101 tcaattcctc tttggtacaa aaaactactt caccattttc tttttccatc tcataatctt
     2161 gtagcatgta ttcaaaccaa cttctttcgg ttggaaattc tatctcgtgg caactctggc
     2221 aaataaaatc accatattca ttgattcggt agcaaccttc atcttctaca aattcatgat
     2281 tacaacaaac acatctagta cctgttagga cttccccatt aatcgttgtt ctaacggtat
     2341 cttcccttgc tacgtgttca ctgctgacaa gccaatcaat gattttcttt gccaattctt
     2401 catcagaatc ggcaaagcct ttaaacgtgt ttctctttcc ttgaaagtag tatttgcaac
     2461 ttgcggtagt ttctatggtt ccataacttt tttggaataa ttcaatcatt tcaccatcaa
     2521 tcactttttg acttttcgct tctaaattag ggaactggtc ttttcttttt tcaaacactt
     2581 atttcacgct ctcttcttct aggtttattc cataattttc tagcaataat ctgtatccct
     2641 taataaaatc tttcgtagca aactcttttc cgctttcaca aaacttgttt agtcctatct
     2701 gttgaccctc aaaatcaaac aatcgcaatc tggttttatc atctagacta aatccatttt
     2761 catcgaacac catctctaaa tgagcatagc catcattgtt atctgaaaac ttcatcacat
     2821 cttcaatcgt ttcttgataa ggttcatagc gcccaccaaa ccctgtttca tcataagcac
     2881 tcactccaga ttcataggta acaaaattga taactcctgt acttctatca aagattcctt
     2941 ttgctaattc ttctcttgat ttatctcgta agtcgtatgc tttattgatt aaataaattc
     3001 cttgattcaa tacgtccatc agcatatatc gaccataccc ccactgataa tgaatcgaca
     3061 cgcttgcttt cgttttgctg ttaccatatt tgactgtaat taacgcttgt gttctttcgc
     3121 ccatcttgtt ttcctctttt ctctaatttt tataaagaac cattttcttg aaatgagtaa
     3181 tagcttgtgt ttttacttgt tacgatgata tggtctaaca attcaatacc aattgttttt
     3241 cctgcgtttt tcaaattcgt agtaaaactt ttgtcatttt cacttggaaa ttcatatccg
     3301 ctcggatgat tatgtgatac tgcgattttt gctgaattgt ttagcaaagc tagttgaaaa
     3361 atctctcttg gtatcgctac tgattgatta attccacctt tgaaaacttc gctataagca
     3421 atgacctcat tctttgtatt cagacacatc actagcaaag attcactagc attgtttcca
     3481 attaagttag tagcaaagtt tgctagacca accgagcttg tgactctata aaaacttcta
     3541 tcttcactga tatatttttg ttcgattttc aagacattct taatcaatcc ttcaccttca
     3601 ttctcggtct tgttttcaac ttcaatagat tcaagactgc tatttcccat atcagaaccc
     3661 aacaaagcat ttctgaaatc caataccttt tcaaagttat cagtcataac catttcatag
     3721 gtgctattcc attcattcaa atatttctct ttgatgagaa tgaacgctct cggtttactt
     3781 ctgccaattc tgatattttg tgtatcgtga aaaatatagt tatcaagtaa agtaccatcg
     3841 tacttttcat agttcccact gatatttgtt aacttgttta ccatttcttg attaacttct
     3901 aaaattctat ctctcatgtt gaacgcttct ttctgttttt gatttttggt ttttcaaaat
     3961 tcttttttgt ttggattgtt ttagagggag gtggtttttt cttcatcgct atttggtttt
     4021 gcattttatg tttcttcctt tcgttcattt atttatcttt ttcgcctgtc ttgccttgtc
     4081 gggagtgatt agaatgcgtt cttatctgcc ggattaatac aagtgtttaa gtaaattatt
     4141 ttcgtaacgc ttgcgtttag aaaaaaattt acgccacttg tattaatcca aagatgagat
     4201 aagctctaaa cacttctgac aaggtatgac aggcgaaaaa gataatgggc gtaaggaatt
     4261 ataaatggca aaaccaaata aagagcgatg aatttcaatg ctttcaggcg tagcttgaaa
     4321 gcctaaaagt cttgaagaat gaaagacttt ttaccttcaa agcagaccta gtaatcttaa
     4381 ataggcgatg gctatttaag attacggtgt tgacccacta aataaacaga aatggaaccg
     4441 atcaagcaaa atcggactag gcgatggcta ggacggtttg cggtggcttt aggaaacgtt
     4501 catggtgaga tgagcatatc tagcaatgtc gggctaagct atgcttag
//
LOCUS       DAZUMV010000170         4544 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000170 DAZUMV010000000
VERSION     DAZUMV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.175"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..162)
                     /locus_tag="ACG8FK_002647"
     CDS             complement(<1..162)
                     /locus_tag="ACG8FK_002647"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5098196.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDF"
     gene            complement(308..1186)
                     /locus_tag="ACG8FK_002648"
     CDS             complement(308..1186)
                     /locus_tag="ACG8FK_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5098197.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1225..2343)
                     /locus_tag="ACG8FK_002649"
     CDS             complement(1225..2343)
                     /locus_tag="ACG8FK_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS5098198.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2340..4439)
                     /locus_tag="ACG8FK_002650"
     CDS             complement(2340..4439)
                     /locus_tag="ACG8FK_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS5098199.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
BASE COUNT         1366 a         1025 c          789 g         1364 t
ORIGIN      
        1 gaagtcaaca tttcgaaaaa tgaacataca ttggtaagca actataacta ttaccacaga
       61 cataaagtaa cacaatatat tactatttaa gtgtacgcca ccagtgaata tcctcaaggt
      121 atacagcgca caacaaaaag aaaaaaggaa acgcagaagc atttcctttc agtgatttaa
      181 tgaaaacaac acgatttgac ttagagcctt tttatttgaa ttcattttcg ctttatccaa
      241 cttcatttga tcatttgatt actcggttaa atacttttga catgagtatc aatgaaacat
      301 tcattaatta tggttgctta tttcttaccg cttttctggc caatgctaat ccgccgatta
      361 ttccggcatt atcacctaat tcgggcgtaa taatgtagct atcaattggt ggtatttcaa
      421 catatccatt tataagctca acaaatttat cctgaacttt tttcttcatg tgttcttgtt
      481 tcattacacc accgccaaaa ataattctat ctggcgaaag catcagtgtt gtattgtatg
      541 cacattgggc aaggtaaaag gcttcaatct gccaaaacga atcgtcagcc aataaattct
      601 ggccttttac acccaatcgt ttttcaatcg ctggtcctgc tgccatcccc tctaaacaat
      661 cttggtggaa gggacagttt cctgaaaaat tgtcctttgg atgtcgcttg atcaacatat
      721 gccccatttc cggatgactg aacccttcca cgaaagtccc attgaacaag gcaccacctc
      781 cgatacctgt tcccacagtg aagtatgcaa cacctgctaa ccctttgcca gaaccagcaa
      841 catattcacc atagcaagcc gcattgacat ctgttgtcca ttcaatcgga atattgaaat
      901 attgctttag cgttcctaca aaattgaaat tttgccaagc aagttttggt gttgatgtga
      961 tatgaccgaa actcgttgat ttgcattgaa tatcaattgg accaaagcag ccgaccccga
     1021 tgcctgccaa ttgttcctta aaaggtttaa aaaaatcaat gacctgcttc attgtttcaa
     1081 ctggagttgt tgtgggaaaa ctgactcttt ccaaaatcgt taattcctca gtaccgactg
     1141 cacagacaaa tttagtccct ccagcttcaa ttgccccata tatcatcata ggttcctccc
     1201 ttcgaatatg tgaaaaacac ttcattaacg cggcaagcgt tctcgatcag tgaaatgacg
     1261 tttagtattt cctgaagctt ctgttgaata ccagtaagcg acactagcaa catcgtcttg
     1321 ccgctcaaat aagcgaacat catcgttgcc gatttgttgt aaagtgaccg ataaatcttc
     1381 ggtaaaacag atggggtctt gaagatgcca acgataaaga ccatgtcgtg gcattgcgtc
     1441 gtcaccaaag gcatgaacgg cattcaattt tcccgtttca aaacgatctc ttgtggcatc
     1501 tctatttgat tggtaaggat accctaaaaa ggctgttgaa tagtttttcg catgtggacg
     1561 tccgaactca tcgtactcat gaaaggccca cgccccgcca aaataatctt ctgaacctgt
     1621 tgaggttacg gtcggatact cactatcccc atcaatgtag aatttaaact cgccttcgcc
     1681 ccaccaataa cgttctaggc aggtaagcgc aaggtaagta cccacgtaat agccttgacc
     1741 ctttaatcga tcaattaact tataatcctc ctgtaatttg gtaatgcgtt cccgatgcca
     1801 atttgcgtga aaatataggc tattttcttc caattcgtcc accaaagcgt aatttatcgt
     1861 ataaaaaaat gaggacatat ccttcggatg taaattctcg atcgtaattt tagcggatgt
     1921 ttggaaaggc atttcgaaat aggaattaaa gccccctgta ggattaacca ctatcggcaa
     1981 cgagttcacc tcgcaacgct ctccaaaccc attacaaaag aaatccccta aaggactttc
     2041 aacagcaggg ctatccgacc catcccaata aattcttaag attaaatcac gtaaaacaaa
     2101 actgccaaat tctgttcgat cacgaatcgt catccacata tgacgaatta ttcccgttcc
     2161 agtaatatcc gcaatcacag tttcttcacc agaagtcagc ggaattgcgc cacgaccttt
     2221 gcggcttggt cctagctcgc ttggcaacat cgcagcttta cctttctcgc ctgttggatt
     2281 ttcgggagtg attgctcgac tctggatttt tttaaattgg gtaatatttt ttagtatact
     2341 catctttttc tcctttgaat gtgaatcgtt tttccctttt ctacgtgaag aatttttttc
     2401 gtatcactta ttttcaatgg cttttctgac aaatttgaaa tactcaactc ggattgcgta
     2461 cattcaatcg aaatgaaggc ttgaccgaac ttcaaattct tccaagaaaa cgttaaatta
     2521 tcaacaggat caatttgcaa tactcctttt ttgaatttca atccgaaata ttgactgatc
     2581 attaggctga caaaaagacc cgatgcccag ccgcatttgc cgcagccaaa gtctcgcacg
     2641 acttctcctt gttgagcctc taagcgatac ggccaccacc accacgaacc gtctaaatct
     2701 gccaaacaaa ttagttcttc taatcgggtt acgaaagctt gagggttgtg cagctcgctc
     2761 attaaaacag tgataaagcc aggaaaagtg gcgccggatt ctaatcccca agtgatgcct
     2821 ttgatttcct cgctataggt tggattgaaa gaactagcag aaaaggccat ggtgttctga
     2881 tataaatgat ctttattatc taaaaattga taaaaaggca tcaacgtagt gtctgattct
     2941 tctccatcat gcatcaataa attaattttt ccatccacaa tcacatcact taaaaagatt
     3001 aacccttgct caagaatcgg tttttgatag tgcttcactg aataggttgt tttctcctca
     3061 tcaccgattc cctccaaaaa ttgctcacca aaaggaccat cgatagtcat ttgtttacaa
     3121 attgattcct ttagtttaca ggcaatgttt ttgtatcgct tgctaagact cagttgttct
     3181 agggcttcaa aaatttctgc aagtccaata caggccttcc aaagacaaag attgctgccg
     3241 gtatgatact ttccaagtgc aaaggcgtct gataaccaaa cagatggaaa aagcattggt
     3301 tcccctgacc gacgttgagc taatatgtcc tcaataattc ttgaagcatt cagtagaacc
     3361 tgcggacacc gctcaaagaa ttttgtatcc ccagtatgtt caaaataaag actagccagt
     3421 aacgcactgg ataacgaatt actcagagaa tgattgatac ctcctgtaaa ttttgaacca
     3481 ggaaacttaa cgccatattg atcaaaccat agaaccgttt tttgacataa ctccggttca
     3541 aacatggtga atggcaatga tgcgtaatac atatccttat tccaaattcg attagtagca
     3601 ggatagctgc cccaattgct gccgacgacc tgtccctgtc gattcatacc gaaactagaa
     3661 aagctttgat aaagtgcacg acgatagaga tggacaactc tatcgtttgg cattgacagc
     3721 tgcccaaata gctgagcaaa atagttcaat gtttccttca gccatttttc gatatcagat
     3781 tcctgaaaag tcattacttc ttcaaaagca tttgggtcca caaaagcaat ggcgaagctt
     3841 gccacttcct gttgtgcgat cgtgtcctta tttccttttt gagaaatcaa cacattttgc
     3901 tgatccgaat acttctcttg atacgaaggc acatctttca ctgaaacttg aaggctttcg
     3961 tgtgtttcat tcacaatgta aacctgttga acaagcatac ttaaacgctt attagccagt
     4021 atgggagcaa aggatactac aatgacctta aacgtcgaat aatgatgaat atatctaggc
     4081 aaacagtctt gaatcacatc gacttcaata cgatgatccg attctgacaa atggtagaca
     4141 atcccgttta cttcaacctt taaggataac gaattggttt gagagagctg tttattgacc
     4201 caaacacctg gtttttcctc agttagtaaa ttttctcgat aaaaggtcaa cgtttgaatc
     4261 gtcccgcaaa catcaaggtt tgctaatgtc ttttgattac ttacatcgaa ggtcaatccc
     4321 tttacatcag gaatattccg atagtagctg tattcgtttg aagtagtcac atttccgtcg
     4381 tttaactcag cattaaataa tgggtgtttg ggtgaataaa ttacttcatt aatagtcatc
     4441 ttttccctcc ttgctaaaaa aactgcagcc tagtagctgc agcttcctac tatgcgattc
     4501 caagtaatga caataaaatt cccaagacga ccattccaaa aata
//
LOCUS       DAZUMV010000171         4469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000171 DAZUMV010000000
VERSION     DAZUMV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.176"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..1961
                     /locus_tag="ACG8FK_002651"
     CDS             21..1961
                     /locus_tag="ACG8FK_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS5098200.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2048..3970
                     /locus_tag="ACG8FK_002652"
     CDS             2048..3970
                     /locus_tag="ACG8FK_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5098201.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(4250..>4469)
                     /locus_tag="ACG8FK_002653"
     CDS             complement(4250..>4469)
                     /locus_tag="ACG8FK_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFS5098202.1"
                     /translation="DYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSE
                     EEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1476 a          762 c          929 g         1302 t
ORIGIN      
        1 tgtagaaagg cggtaggaaa atgaaaaaaa ttattgcaat cacatcctgt ccagttggta
       61 ttgcccatac atacatggca gcagaaaact tagagaaagc ggggaaagca gttggcgccg
      121 aggtaaaagt cgaaacacat ggttccatcg gtattgaaaa tgaattgact gcaagagata
      181 tcgaagaagc agctggtgta atcatcgcag cagatactaa aatagataaa tcacgattcg
      241 gcggtaaacc tttgataacc gtaggtgtgc aagaaggaat ccaccatgca gatgagttgg
      301 tgagagatat tttagcagga aaagcgccag tctataaaag tacggaggca attatttctt
      361 ccgaaaataa aagtgaatca gaaaatttag gtaaaaaaat ctataagagt ttgatgaatg
      421 gcgtttcgta catggtacct ttcgtagtta caggagggtt attgatcgcg atttctttaa
      481 cattaggagg aacagctaca cctgaaggga tcaagatacc agaaggaacg atttgggcaa
      541 cgatgaatag tatcggaagt atcgctatgg ggttgatggt tccaattcta tctgcattta
      601 ttgcgcaaag tattgcagat cgtccaggac ttgttcctgg atttgttggt gggatgttag
      661 cggcaaacgg agcattatat ggtagtgatg cgaatgcggg attcttaggc gggatcatca
      721 ctggattttt agcaggattc attgctttag gaatcaagaa agtgcgagta ccgaaagctt
      781 tacaatcgat catgccaatt attgttatcc caattttagg atcattggca gttggttttg
      841 tgtttatcta tgttattgga gaaccagtag ctcttctatt tagttcattg actcactttt
      901 tagcagggat gcaaggagga agtgaaattg ttctagctat gatcttagga gcgatgattg
      961 cttttgatat gggggggccg ttcaataaag tagccttttt gttcggatct ggcttgattg
     1021 cagaaggcaa ttattccatt atggggccaa ttgctgtagc tatctgtatc cctccattag
     1081 cattggggct ggcatcattg attttaaaaa ccaaatttac gaaaacagaa cgagaagctg
     1141 gtaaggcgtc cttagcaatg ggactgtttg ggattacaga aggcgctatt ccgtttgctt
     1201 caacagatcc actacgggtg attccaagca ttgtaatcgg ggcaatgaca ggaagtgtga
     1261 tcgcgatgct ttcaggagtg acagatcatg tagctcatgg cggtccgatc gtagcggttt
     1321 taggagcagt ggatcacgta gccatgtttt ttgtagcagt gattgctggt attgctgtaa
     1381 ccgtgctaat gttaagaatc cttaaaccga cattacaaca aactgcggtt gctgcagcaa
     1441 atggtgtgac agtagcagat ctatcagaga ctgaaacaag tgatgtttat acgaaaactg
     1501 aaacttttga ttctatcacg gatattatgt cagatgaagc aatcgtttta ggtgaaacag
     1561 caacaacaaa agacggagta atcgaagatt tagtccaacg tttagaaaaa acagcggcga
     1621 tcacagataa aaccagattt aaagaagtca tctatgatag agaacatgaa agtacaacgg
     1681 gaatcggtat ggggattgct attccacatg gaaaatcagc ttatgttctt aaaccaacag
     1741 tggcatttgc cagaagtcaa tctggggtag attggcattc tttagatgga aaacctgcac
     1801 atatgatctt tatgattgct gtacctgaaa acagccaagg tgatatgcac ttgaaaatac
     1861 tgcaacgtct atcacgtaaa ttgatggatg atgacttccg tcaagcactg atggaagcgc
     1921 cagataaaac aacagttcac caattattaa gcgaaatgta acataagatg aaaaatcgac
     1981 cgtgatgaga cataatagat gtgttatcat gatcgatttt ttatttagaa gggagtttct
     2041 gcacattatg aatcagcgtc agcaatcttt gatctatcaa ttaagtgaga acaataatta
     2101 tataacaaca aaaaagctag cagagcattt ccatgtgtca gaaagaacga ttcatagcga
     2161 tttgaatacc attgaaactt ggttgagaga caaacatctg cctttaatgt ttgaaagaaa
     2221 aaaaggtaca ggtatcttgt tgaatggtct gcctcacgaa agggaacaac tgaaacgagc
     2281 gcttaatgaa gagactcaaa cagaaatgga taaaaaacag cttcgtcagt tattgatttt
     2341 ccatctgcta gtagcaaaag acggatttag tcttgaagaa ctttcagaaa aactatatgt
     2401 tggaaaacgt acgattcgta aagaattgac agagttgcaa agcttttttg agtatcacca
     2461 tttacagcta gtatccaaaa caaaactagg aacttttctt aaaggcgatg aacaagaaaa
     2521 aagacagttg cttgttaaga cattacgaaa tatgcagaaa gaagatccac aaagtccgtc
     2581 gttgaaagaa ttcttcgcta aagatacatt aaaagttatc caacatacat tacaagatgt
     2641 tttttatgaa aaccatttag aacagccgaa tggactggct agtgtggaga ttcacatcta
     2701 ctttatgctc gagcgaatga aacagtacca gaaagtgaaa ttaagtaaag acgaaaacga
     2761 agtcgtggaa cacacccaag cacaaaagtt aagtagtcaa atattagcaa aactagccac
     2821 catctatcca attgaatttt ctcctgatga aatcaattat ctggctttgc gtatcgccaa
     2881 tctttttaca aattcacaag cagccacacg ttttcaaaag gaatctagcg cacttgctga
     2941 tcatttgatc gaacaagtgg aacagtttct aggctattca ttaaaagaag atcagctact
     3001 aaaacaaaat ttaaggtctc atttatcttc aacttatttt cgactccatt atggtttgca
     3061 gatttctaat ccattgacga aaaatgtgtt tagtacgtac acacaacttt tcttagtttt
     3121 acagttaatt ttagaagatt attttgaaaa ggaaaatttt tatgttccac aagaggagac
     3181 agcttattta acgatccatt tccaagcagc gatcgagcga cacaaacatc agaaaagtag
     3241 aagatatcaa acggttttag ttagtgaata ttcaaaagct atggctactt ttctcgaagc
     3301 acggctgaac agagaacttc cagaattaac gattatggat ctagttgaat ataagccaga
     3361 attaaagaga caagattttc cctctgttga ttttatcttg gctacgttac catttaaaca
     3421 tataagcatt ccagtaatcg agatctcacc aatgatcaca gaaactgatc tagcctattt
     3481 gacaaaatat atgctggagc atgtgcctat caagaagaaa aaaacatttg atctagcaag
     3541 ctttactcat ccatttctga tctttcctca acttgaacgg acagacccag ccgatatttt
     3601 aaactttatg ggaaacgtct tagtggaaca tcattatgtg gagccggaat tcatcgattc
     3661 tgtcttggaa agagacagtc actccagtac tagagtagca ccatttgtaa caatccctca
     3721 cggtaatccg ctgtatgtga agcattcgat gatttctatt gctacaatga aagaaccagt
     3781 cctatggcac ggggaacaga tacgtatcgt actgctatta gcaataaaga aaaatgattt
     3841 gaaacatgca gagttcaaaa aaatattttc tgttattcac tatctagaaa agcagccaga
     3901 acaaatggct cagttgatgc aatccaatca tccgctagaa atcttaacgt tgttatctag
     3961 ttatgaataa gtagcgatta aaaaactgcc tttatattta taataattct atctatttat
     4021 ttggaagtag aaaactaaat acaaaaaaag aggatgtggc caaagttgtg gcacatcctt
     4081 ttattttgag tttcttataa ttttttatag ttgcttacga tgtcttgact actggtaaac
     4141 ggtgttcaag agacaatttg tcggcaataa ggcacaattt tgtaaaatat ggaaagctat
     4201 ttttttaaca ttgtttctta gtgcttgaaa aatcattttg aatacttctt tatacgatcg
     4261 atgtacgttg atttaagtct aaaacttttc ttttgattcc ttctctgact tttctgaatt
     4321 ccgttaatat ttcttcttcg ctcccgacag cttgtgcagg atcttttagg tcccagtgaa
     4381 ggtgttcgtg attgtttgga acggcagggc acttgtcttt ggcatcacca caaagtgtaa
     4441 tgatcaagtc tgcctgagta aagtaatct
//
LOCUS       DAZUMV010000172         4456 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000172 DAZUMV010000000
VERSION     DAZUMV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.177"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..938)
                     /locus_tag="ACG8FK_002654"
     CDS             complement(42..938)
                     /locus_tag="ACG8FK_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5098203.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(931..1611)
                     /locus_tag="ACG8FK_002655"
     CDS             complement(931..1611)
                     /locus_tag="ACG8FK_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5098204.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1612..2394)
                     /locus_tag="ACG8FK_002656"
     CDS             complement(1612..2394)
                     /locus_tag="ACG8FK_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5098205.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2387..3304)
                     /locus_tag="ACG8FK_002657"
     CDS             complement(2387..3304)
                     /locus_tag="ACG8FK_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5098206.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3468..3722)
                     /locus_tag="ACG8FK_002658"
     CDS             complement(3468..3722)
                     /locus_tag="ACG8FK_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098207.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1310 a          955 c          773 g         1418 t
ORIGIN      
        1 gataatacat ttttctacta gttctcacta atcttgtata attaaaccct ccgcaacttg
       61 acggagatgg agaagatgcc cccgtctaat ctagcggtca tctctcctcc cattccttcc
      121 accaattttt ttgacaggta aagcccgatt ccgctagatt ccgtatttgt gcggctggta
      181 ttttcggtat aaaaacgctg gaagactttt tcaacagagg tttgcgttcc tggtttgata
      241 ggattggcca ctgttaatcg aatgaatgga tcctcttttg aaaatgtcac ctttgcttgt
      301 ccatctgcat aacgcaataa attctgtatg atattttgca gcactcgcag caacatgagc
      361 ggatctgcta atacagttgt atttatcgag tgttcagcga acgctacctc gatatttttc
      421 tcagaaaatt gttcataaaa agagaaaaat acttcttcca cctctttaga aaaagaaagc
      481 gtatggagat ccatttcttt ttgcttcgtc tctattcgag caagatcata aaactgatcc
      541 gtcatactga gtaatcttgc aacagattgt ttgatgatcg ttaaatactt ctcccgtttc
      601 tccgtatcct ctacctgttc cagtaattgt acataaccgt taatagcagt caaaggagtt
      661 ctgagatcat gagaaaggcc gacgatcgat agtctgatgt tttcttccat ttcttgaaat
      721 gcttgtcctt tttgttcatt ttcatccaac catgtattca gtgcatctac taggtccatt
      781 agattctttt ctcgaaattc gactgaaaga cgaccaccat attcttgtcg ttcagaaaga
      841 gtggcgattt tcttagttaa tctcttgatt tccctttgca ggaagaaaat aaaacttccc
      901 ataaagataa caacgagaag caacagccat ttaatcaaat ctaaacccca ctccccacac
      961 cgtatgaata tacacagcat gtgcctttgc tagcttccct cgaagattgg aaatatgcat
     1021 attcactgtt ttctcactat caaaatatgg ttcttgccaa actgtttcat aaagatttgc
     1081 tcgcgaaaat acttttttcg gatgttctaa aaaaagcttc agcagatcga attctcttcc
     1141 tgtcaaatgg acagccacac cattcacatt tacatccctc gactctttgt ccagtaagat
     1201 ttccttgtaa tagatgcgtt gcgacagatc ttttacttgg acagaagtct gatgacgaag
     1261 ttggatgccg attcgtaaca aaagttcttc gatatcaaat ggtttcgtca catagtcatc
     1321 cgctcctgct gccaacaagg aaactttatc tctttgatcg ctttttgctg aaagaacgat
     1381 gatcggaaca ttcgatgtct gtcggatttc tgctaacaat gcttcacctg aaagccctgg
     1441 aagcatgaga tctagaagga tcaaatcgac aggatatgtg cttaacaata attttccttc
     1501 cgtacctgaa taagcatcaa gaagtttgta tctctgttca agaacctcct ccagtaagga
     1561 atgaacagct tcatcgtctt cgataatcaa aatagtttcc agcaggacca cctacttaat
     1621 gtcagatttt tggaaaataa acattcctag agctgatgat accacgattg ttactagact
     1681 gaccagaatc attttcggtg cttgcgaata agttgcttgt tgcgcaaagt ccgtatacat
     1741 gagccacgtt tccgtaaacc actccgttag aaattccaag cctttgattt ttgcgaaaac
     1801 accgggaatg aaggccgttc ccatgatata aagaaaagaa agaagcaaac tgacactggt
     1861 acttctagtg atgaatgtca aagccactgg aacagataat gtccctaatg tcagcagaag
     1921 caatacagga aatactttta gaacataatt tccatagttt ttgattccct caacttcacc
     1981 aaatccgaaa gcaagtgtgt aagcaagcat ggctagtccc gtgatcaaaa ccacgccaag
     2041 tgttgtgatc aaaagcgtaa cgagaagttt cgacacataa taagtcattc gattccgtcc
     2101 taaagataac gtattgcgaa tcgtaccgtt ttgaaattct tccccaatat aaaagcacgc
     2161 aaatgctgcg aaagccagaa gaatccatgc agccattttt tctaccatta aaatccctgt
     2221 tgcaccgttg atgactgtat ccgttgcttg gtttaattca ttgccgtatt ttgtatgcat
     2281 ataccattcg ttccaagaat aaaaaagcgg aagtacgacc atcagtccta gtgagaccca
     2341 cattttacgg ctcttccata cttttatttt ttcagcagtt aatagattaa acatgtggat
     2401 tgcctccagt taattgtaag aaataatctt ccagtttttg cccaactagg tgaataccat
     2461 ccacaggaac ttttgcttct accaatgctt gattgatggc tgctacttgg tcaattcctt
     2521 caaaaagctg gataacacgg ctagattgta caaaataatc cctataacca agagagtcga
     2581 gaaccgttac tgcttgttcc gtcgccgaag tgtcaatttt tataaattga cgagattctt
     2641 gtgccaattc ttctttcgat aattctttga tcaagtgtcc ttcatgcaaa ataccatagt
     2701 gagtggcgat ctgtgacaat tcatctagca agtggctaga aagcaagacg gtgatgcccc
     2761 gttcttttac caaacgttgg atgatttctc gcatttctgc catccctgct ggatccatcc
     2821 cattcgtcgg ttcatccaaa atcaaaaatt ctggattcgt gatcagtgca tttgcaatag
     2881 ctaaacgctg gcgcatacct aaagagaaat tctttgcttt ctttttccct gtattttcca
     2941 atcgcatcat tttcagcagg tcttcgattt ctctatccga cacaccgccg ttcgctgctt
     3001 ggatacgtag gttatctctt gcagttaatt ctggataaag agcaggggtt tcaatcgatt
     3061 gacccatacg tcgtcttgct gcctgcaaat ccgtcgttcc aaataattcg atcgaacctt
     3121 catcaatcga gatcaaaccc ataatagttc tcataaaggt agacttaccc gctccatttt
     3181 ccccaatcaa tccatagatc attccacgtt tgatttcgat tgatacttta tctaatgctt
     3241 tggcagcacc gtattttttg gttataccag ttgctttcaa gaccgtttca tttccaaaat
     3301 ccatacttat ccttctttct tattgatgag tgaagtatag ttgacgggta taaagaaagc
     3361 agccagaaat gtataaagaa agtgtaaatt ttccaattga atgtcaaaaa aaacaatcgg
     3421 acaatacgtg tctttcctat tatcaggcag tttgttatac gcttgtatca gtttcctgat
     3481 tctttctttt cgaccaaagt cgtttctctg tattcctctt cactgaaaat ttttgcttct
     3541 ttcagatcaa gcttgagtaa ttttaccaga ccggttataa ggataactat agcaagactc
     3601 cctcccagaa aaattagact gtctcgcaga cgtggttgcg ttgtcttcca atcaaagagc
     3661 gcaccaaaca gtgctaatcc aagtggactg gccaccatgg taatcgttgt tatcgttgac
     3721 ataagtcgtc cttgtatatg cgttggaact tctcgctgaa gccagaccat ataaaaagca
     3781 aaagaaaaca ttaatcctta aaaacggact tatgaaaaat aatacatgcc gtttgggatt
     3841 tacaattagc taccaaataa gaggttatga caaaagactt ttcacaatcc cttcttccga
     3901 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatttat caaacatgag
     3961 aagcaatttt tgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctca
     4021 gaagcggata aataatattg tttgatgata ctttatcttt ttcccgtttg tttatagatc
     4081 gaatgatatt atagaccaag aatcctacct attaacatag ctaaaaacac aaaaaaggca
     4141 ctttccaaat tgtggaaagt gccagaaaag ttaggattaa ggtaactgcg ctcagcattg
     4201 cttcgcccag tacgattctc gtaactcgtg aactcgaacg ataatcgcaa ttaacgtttt
     4261 gagaactgtg aagctttacg ggctttctta agacctggtt ttttcttcgt atcattgaat
     4321 aagtttgaaa aaccttgatt tataaaggtt ttcccgtaaa tatattttat tatatttgat
     4381 taaatttgaa atgtttgtgt tctaaaatgt gttctaattt aaaggcagta tatttcgtat
     4441 tttagattaa attttt
//
LOCUS       DAZUMV010000173         4301 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000173 DAZUMV010000000
VERSION     DAZUMV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.178"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..1405
                     /locus_tag="ACG8FK_002659"
     CDS             104..1405
                     /locus_tag="ACG8FK_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5098208.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1432..1692
                     /locus_tag="ACG8FK_002660"
     CDS             1432..1692
                     /locus_tag="ACG8FK_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5098209.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2691..3926)
                     /locus_tag="ACG8FK_002661"
     CDS             complement(2691..3926)
                     /locus_tag="ACG8FK_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5098210.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1251 a          685 c          883 g         1482 t
ORIGIN      
        1 aatatattga aataaaattt cttaattata taattagtta tgaacattct ttgcgttgtc
       61 atgcaatgaa tggttcatat tttattgaaa aggagtggtt ttagtggaaa agctgatgaa
      121 atgggtcgaa gagaaacttg tcccaccaat ggcaaaaatt ggtacacagc gtcatttatt
      181 agcaattcgt aatggggttg tttcaacatt gtcattgatt ttgattggga cattttttat
      241 ggtctttatc aatttaccgt ttcctggatg gaatgaattt attgcccctt attcggcaac
      301 gattgtttta ccctttagga tgacgatggg attgatggct atttatgcaa cttttgttat
      361 gggttcggat ctagctaaat catatggatt agatagtgtg acaggtggta tattatcttt
      421 aggaacgttc tttatgttac aagttcctgt aaacgtttta actcctgaag aggcacctct
      481 tggctgggtc ttacctatga gttctttggg agcatctggg atgtttgcgg ggatcttatc
      541 tatgattttt gcagtggaag tatatcgttt ctttaaaaat cgaaatatta ctatcaaaat
      601 gccagaacaa gtgccacctg ctgttgcccg tagttttgag gcattgattc caggtgcagt
      661 cattttaaca attacttggc tgattcgaag cgtcgcgggc tttgatgtaa acgctgcctt
      721 attatcgtta ttcgagcctt taaccaatat tttagggaat aatttgcttg gtgttttact
      781 accaatgttt ttgatccatt tgttgtggtc atttggtatc catggcatga gtattgtagg
      841 ggctgttgtt cgtccaatgt ggctgatcat gttggatgag aatgcaaagg cattggcaga
      901 tggtacagct gccacccaat taccctatat cgctcctgaa cagttttatc aatggacggt
      961 aacaattggt ggtgcaggag caacgatcgt ggtatctgtt ttgtttatat tcttctgtaa
     1021 atcaaaattc ttgaaagagg ttggtcgttt ttctattatt cctggtatct tcaatatcaa
     1081 tgagcccata atttttggag ctcctatgat gttgaatcct tacatgttca tcccgttcaa
     1141 cttagttcct ttagttttga ctattatcag ttattttgca gtcaaattag aaatggtcaa
     1201 tagatttact gttttgccaa catggacatt accagctcca atcggcggtt atctttcagc
     1261 aggaaatgac tggcgtgttg ttatcttgat catgatcaac acattgatag catttattat
     1321 ctattatcca tttgtaaaag cttatgataa aaagatgctg tcagatgaac aaagtcgtgg
     1381 agaaaacgaa gtccagactg tctaagtttg gtttttagaa ggtgggaaac aatgtattgg
     1441 ctgattttat tttttgtttt tatcttttta ttaacggcta gtcaccttat attgaatatg
     1501 ctagctacat atcatattca aatcaatcga tggatttggg cactggcatc atttttgatc
     1561 gtgattcttc caaagatcat tgtaccgcat atgaatgttt tgttttcatg ggggacatat
     1621 gtactttgtg gaatttttgc tatcaatttt atgatcgagc agcatagatg gtttgtcaca
     1681 tcgaagcttt aaatgttcta aaagtggttg taacagaagt ggttagcttc aagaaataag
     1741 aagaaattta cgaaaattgc ttcttaaatt tttgtgaaat gttggcttat ttttcgtttg
     1801 gttgaactta ttaccgcaga tgcaagcttt tgaccaccgt ttattcggaa tatgggactg
     1861 tgacaaggct tttgtcacag ccctttttta cataaagatt attattgttt taactgtttt
     1921 ggcagaaaga aggaggaaaa aatggaaatt ttatcagcaa tggatttaga attagtagat
     1981 ctactaccaa agtgatcaag tcaaatatag atttgggagc ttacaaccat atagtagggg
     2041 ctgcagtatg gtgtatgaat ggtaatatgt atgatgtgag tgtttattca aatcatgaaa
     2101 cgtgtataga aataattgtg ctgggaatag caatcagtca tggtgaacgt gacttttagt
     2161 gtatcgttgc tgacgacata gaacattctg acatgatcta ctcaatatgt ggggattgct
     2221 ggcagtttat ataggagtat gcatcggatt gcgagatcat tgtctcaaca gaagagggag
     2281 gttgattatc aaagataaag tggtaaatcc tacatttgat ttagtaattg ttgaggggac
     2341 tagcggcaca aatctctaca aagcgatgct tctagatcta tatgacacat tttaagaaaa
     2401 tgtatttgcc taattattat ggtctttctt ttgaagaaac gttgagatga tccaaccaga
     2461 gaaaggtgtc aaagaatttg actaagaata aatggcagac gctacctatg ctcatttgga
     2521 tagcgtcaga tcttttattt taaggatgat tttgtgtttt ttagacaccg taatttaagt
     2581 aactctaact tttcaatcca ctatttactt tttttgtatt ttcactgtct gggtctcgaa
     2641 tagcgaaatt actgatgagt ctctattaaa aaagcttttt ctacttgaaa ctatattggg
     2701 tggttgctca ggaaggtttc gatttcttga attgcttgtt gtggaccatt tattggaaaa
     2761 acggcttgca gcgtgcgtgc ctgctgacga atcttgggtg aagaaacttc gcgcagagca
     2821 atctgcagtt ttctttcagt cactggtttt gtgagggcga ttgcgtttcc gtattttaca
     2881 caatagtcaa tattacagct ttgctcaaat tgcatcccca caccgataaa cggtttgccg
     2941 gattcacagg cagtttggac ggtcccttcc ccgccgtgga taaaagaaaa atcaatgaat
     3001 tcatgcaaaa gatgactaga cagatattct gttattaaaa gatcttctga gtcagattga
     3061 atgtcatcaa gcagatagtc aataggtgca ataaccgcta gatccggttg tttttgtaag
     3121 aagaacaaaa ttttgcgtaa catacgcgga ttgcctgaac tacccattgc gaaatagata
     3181 atacgttttc tttcttgttg ctttcgctgt ataaaagtta gcacttcttc aggcaaggaa
     3241 gtactgagct tagcaaaaat tggaccaacg attcgataat tttccggcag ctgttcttct
     3301 tccaaaaata gtttaggcgt tgtgatgaga tttagatcag catccatcat ttgtaaactt
     3361 gtttttggta aaggaagcat ttctttttgt gcagcgtgtt tgaaggcatt ggggatccat
     3421 cgggtattta ctatacgctt ttttgtccag tgccaaaggg gattgcttaa agggccgaag
     3481 aaacgtaact cgtggggcat cagcgcatgc tctgattgaa aaaaaggttc actcaaagca
     3541 tagggtttaa catatactaa aggaatcttt ttattgcggg ccgataaaaa aattgtcaca
     3601 ttcgaacctg tcacaatcag tcgaggctca aacttagcaa tcagactctg ttcactagtg
     3661 atacgagagg caaccatttc tttggtaaaa gggtttttaa gtgtacgcaa ctggtcaaat
     3721 tttatgattt gttcttcttg ttttttcgta aggatcggcg ctagtaaatg atactcaaag
     3781 ccactttctg tgatcaaagg agcaaaagtt ttagaaaagc caaagaatac acagtaataa
     3841 cggtctttca tagctttggc aacctcaatc attcgtgttg tctcagctaa attgaatact
     3901 gcaggagcaa aaagaattgt cttcatgaaa tcctccttat tggaatgagt ctatcccttc
     3961 gtttcttact agtatactaa aaaacacagc gttcgagtag cctatgtata ttttgaatat
     4021 taaactgtct ctcgctgttt tcacaacttc taaatttaaa ttttgtaatc aagttgaaac
     4081 tccaattttc aaaaaaacac gcaacaattt tttgatcgat ttatatgttg tgccagataa
     4141 atacgagaag ttgtgtcatt aaaaataaac atgagaacat aaaaaaataa aataattata
     4201 ttaattgata ggtgtgctgt cgtcattata cacacgtaag aaagaaatag ttatgtttaa
     4261 aaatattctt agactttctc gccatgacaa ttttcactat g
//
LOCUS       DAZUMV010000174         4172 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000174 DAZUMV010000000
VERSION     DAZUMV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.179"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..559
                     /gene="bacA"
                     /locus_tag="ACG8FK_002662"
     CDS             125..559
                     /gene="bacA"
                     /locus_tag="ACG8FK_002662"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFS5098211.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            1849..2772
                     /locus_tag="ACG8FK_002663"
     CDS             1849..2772
                     /locus_tag="ACG8FK_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5098212.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            2788..3327
                     /locus_tag="ACG8FK_002664"
     CDS             2788..3327
                     /locus_tag="ACG8FK_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5098213.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1546 a          621 c          706 g         1299 t
ORIGIN      
        1 tactaacctt taaaataaca cgttttataa aataaaccct cttgacaata tttaattttc
       61 tgattattct aaatatatac agaaagcgct ttcctaggat tttatatcta aggaggaagt
      121 aacaatgaaa aataaaatta ttaaaattat tacttgtcta tttttgttcg gtatgattgt
      181 tggagcaagt actccgttaa atctgtttcc ttttagtgct agaggtataa aagtggaagc
      241 tgcaagttca cgatataatc ataatcatcg tggatttact tgctataaaa tgaactatta
      301 tgttactaat aaaatgtgta gagatttgaa aaaaaattat aataagctaa agactcctgc
      361 tcaaattgca tcttttattc ctattggtgg agttggaaca tgggttatta caaatgcttt
      421 tagtagtgct gttgatgcaa tgaatgtctt tgtaagagct gcaaatcaag gaaaaggtgt
      481 acaattaaca tataatgctc actttagcaa tacaacatca tatcaataca atgattacgc
      541 tcgttatgta ataaaataat cagaaagtga aactatctca tgaataaaaa gattttagct
      601 gtaattattt caataatagt tttaatcact atgttcttca tatttaggct atttttcaat
      661 ataagcttac aaaaaagtat tctttatcta attcctatag catttatctt agcaattttt
      721 agaagtattt acggaaataa aaactctaaa tagctaaaaa taaatcatac taaattttac
      781 aaaaagtggc tagcattttc tagccacttt ttgtattgtg gagcgtgggt tttgaggggg
      841 attcaagggg gagcttgcgc ccctttgaca agcggttatt tgcaccaaat acggaggtat
      901 ttggctagca aatatagagc ttgccaaacc cttttagtga gttttgtaga aagtaccttt
      961 aaaggtactt tcacattacg aacgatacgt ccgtaggacg tactgaaagg gtgggactta
     1021 tgagcaaatt aatgcaaaat ttggctagca aaaaagctaa aaaatatatg ctaataatcg
     1081 agcgaaataa acactttttc aagtgagcga atccgccatg acaaagctga agcagtttat
     1141 ttcagctttg tcatggcgtg tgccgacttt tttaaaagtc taggcacacg agcaaatatc
     1201 tttatcaagc tctaatttgc gttttaagac gtttgaaacg cctaagtata taaatatata
     1261 agaataggtt taagagtgct taaatcgaat tttagagggc atttagacaa ttttagggtt
     1321 gttagggctt gccctgaccg cctgtaaggc gttttaagaa caagataagg gatttatact
     1381 aggactgtct taaaatgtct taaacgaacg ctcagagctt ctcaaagggt tccttatgaa
     1441 cggcttttag caaaggagcc ggaggcgact gcgcagtata atgtgagagc agagcgatca
     1501 caccttttta gcgaaccgta gggagcaagg tttggaattg gcagttttga taaattcgcg
     1561 aacctgttta gagaaaaaag gagtgatttt ttaggcggga aaattagcct cccctctttt
     1621 tatatctctt tttatatctc tttttatatc tcttttatgt cggctgtgtc cttactctcc
     1681 caagcaattc agagtttttt caagtaggtt ttctacgctt ttcaagtagg ttttctacgc
     1741 ttttcaagta ggttttctac gcttttcaag taggttttcc gacattattg agtattttta
     1801 taaagtatgt tacttttatt tcatacttta taaaagtagg agaagcttat ggcaaatgaa
     1861 attgtaaaat atgaaaatag attaaatcat attcctctga gaaaatttaa tagtcgtgaa
     1921 atgaacttat ttttttcaat tgcttcacgt gtacgtgaaa aaggtgtaga tgagattatt
     1981 ttttcatttc acgatttgaa aaaactaagc ggttatacag aacgtgggga acaatttata
     2041 aaagatctta gtagcacgta tgacaaatta ttaagtatta atgcttggaa tgatgatgga
     2101 aatactttaa ctaaatttgt tgctttcaca aaatatagca ttatacgaga taaacaactt
     2161 gttataattt cagttaatcc agaatttaaa ggacttttta atcaattaaa gaactggaca
     2221 agatttaatc tagagcaatt tactaatttg gatagcacat attcaaaaac tatgtttcga
     2281 ttattaaaac aatatcgaac tcaaggctgg actgaatttt ccaaagaaaa attttttgat
     2341 ttactagata ttcctaaaag ttattggaat agtccgtcaa atattgacaa atttatctta
     2401 aggccaatta aagaagaact cactccctta tttaaaggat taactattcg taaaaaatat
     2461 ggtaaaggtc gaggtaaacc tgttatcggt tatcgtttta cttggaaagc tgaaattaac
     2521 catgctgacg atttttccaa agggaaacaa gaagatttac gtataaaatt atttaatatt
     2581 gatcacaatg gagaattaac acgagaagaa aaatggcgcg ctaaagatcg aatactaaat
     2641 cttcccttag gcactcatga agctgatttt aaacaacaac aacaaactga agaaaaacag
     2701 gcagaaaaac gagaactttc aaacaaactc aaacaagatt tactaggaaa tttacaaaac
     2761 ttattcgatt aagcttaagg agtatttatg gcagataaaa caatcaaaga acttgcggaa
     2821 gaattaggag ttagtaaaac agcaattaac aaaaaagttt ctgacaaaaa tcgaaaactt
     2881 tggtttgcaa aaaacggaaa caagtttgtg attaacgaaa ccggtcaaaa agctataaaa
     2941 tcaatgtttt tagtcgataa cgcaaactta aatcgaaaac tagttggcga aaaatcaaaa
     3001 accaaaaacc ataaaaacac caaccaagtt tccgaattaa aactcctttc tttcctagaa
     3061 aaacagttaa atcgaaaaga tatacagcta caagaaaaag ataatcaaat aaaacaaatg
     3121 caaaaattac tcgaccagca acaacaactc actctacaag cgaaccaaca aattgagaac
     3181 ctacaagaac agctccattt aacttataca gaagaaactc caacaaccga atcaactgcc
     3241 ttttctgaaa aaaaagcaga caatacacaa ccacttattg aaaaaaagtg gtggcaaatt
     3301 tggaaatcta atagaaccaa tctctaaaat tttctagaag ttagttcgga tagtagagga
     3361 aaaagttgta ctacctgatg gggagatcac tcaaattttt atgagaaaga aaaaatagtt
     3421 tacaactgta aacaatcaat gtttatagtt gtaaactatc aaactatatg gtgacttatt
     3481 gcttaaaata ggttgaaatc tcactcaatt tttggtggaa aaacaaaata aattctaatc
     3541 gattttgttg aaaaagtcat aaaaaaacaa atgtgatttt aacaaaattt caaaaaaatc
     3601 gtttgaaatt tcgtttattt tttttgagaa ttttgatcat cgtagggtca ttgtagaatc
     3661 attatgagat catcgtggga tcatcgaata aagtatggga aaggcttgcg gtgcttggta
     3721 ttaagggcat tctccgcctc ccaggttctt gaccattttc tctcaacgtg gtaaaaatcc
     3781 cacttataag agaaaatggt caagaaccta aacggtcggc gaagaaaaca taccaagcac
     3841 cacaagcaaa aaaagttaga gtaacttcca aaaaagaaaa attttttaga acaacttttg
     3901 aaaatttaat tttgatttat atccgttcaa tttaaacatt tgataaactg aaggctagta
     3961 tttaaaatgt gtatacaagt ggtagcgtta aaaaaagtgg aggtataacc tatgttaaaa
     4021 aaaactgtgt tggtactggg ttctatttct ctaggaatat taggttttag ccccatgcta
     4081 ttatccgtaa ttggaaaaaa caattccaac ggagaactta aattaaattc aaattattac
     4141 aacagtccta acgaagaatt gtttgtaaaa ga
//
LOCUS       DAZUMV010000175         3982 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000175 DAZUMV010000000
VERSION     DAZUMV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.180"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..1469
                     /locus_tag="ACG8FK_002665"
     CDS             120..1469
                     /locus_tag="ACG8FK_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5098214.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1597..2187)
                     /locus_tag="ACG8FK_002666"
     CDS             complement(1597..2187)
                     /locus_tag="ACG8FK_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5098215.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2334..3686
                     /gene="brnQ"
                     /locus_tag="ACG8FK_002667"
     CDS             2334..3686
                     /gene="brnQ"
                     /locus_tag="ACG8FK_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5098216.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1141 a          731 c          777 g         1333 t
ORIGIN      
        1 tttttatgaa ttactcataa aaggtaaatt tctgaaaatt tcggaatctt agttgtgttt
       61 ttcagaaaac tctcttaata tggaagtaat taaacttttt tagttaggat gatttaaaaa
      121 tgaatgaaga gcaagaactg cagcgtggac tgaaaaatag acatgtccaa ctgatttcta
      181 ttggcggagc gattggaacg gggttgtttt taggttccgg aaagtctatc cagctagctg
      241 gcccttcgat attgctcgcc tatcttatta ctggatgcat ctgttttttc attatgcgtg
      301 cgttaggcga acttctgtta tcaaacacca ataaccattc ttttctggat tttgttgcag
      361 aatatcttgg caaaaaagcg gctttcatca ctggctggac gtattggttc tgctgggtat
      421 cgattgcaat ggccgattta acagccatcg gaatgtatgt acgctactgg gcgcctggtg
      481 ttcctcagtg gcttcctgaa ctgattgcat taggtcttct tctttgcttg aatatggtgg
      541 ctgtctcatt gtttggtgag ttagagtttt ggtttgcatt gatcaaagtt gtagcgatca
      601 ttgcctttat cctcgtagga gcttatatga tcctcaccca ctatacgaca gatattggca
      661 cagccagtat cacgaatcta tggtctcatg gcggattctt tccaactggt gcaaaaggat
      721 ttatcttagc tttccaaatg gtaacctttg cttttgctgg gatagaatta gttggattag
      781 tcgctggaga aacagaaaat cctgaaaaag tgttgccgga agctatcaat aatattccta
      841 tcaggatcat cttattttac ttaggatctc tttttgtcat tatggcaatc tatccatgga
      901 acagcttgaa tccagataac agtccatttg tagaagtatt ttcggaaatt gggatcacga
      961 ttgctgcttc cttgatcaat ttagtcgtat taagtgcagc agcttctgcc tgcaacagtg
     1021 caatatatag tacaggacgt atgcttcgtt ctttagcgca agaaggcagt gcccctaaaa
     1081 aattcaaaaa gttaacaaca catcatgtgc cagggaatgc tttgacattt tctactatcg
     1141 tcattttcat ttctgttatc ttgaattacg tgatgcctag tgaagtattc acactagtaa
     1201 gcagtattgc cactacttgt ttcttgttta tctggagtat gcttgtctac acgcacatga
     1261 aatatcgtaa aagtatcctc ggcaaaaaag ctcattcatt taaaatgccg ttgtacccat
     1321 ttagtaatta tctggtattt ctttacatgg cctttgtgtg tgtcgtcttg ttcctaggaa
     1381 aagatacgag aatcgcttta ttactaacac ctgtgtggtt cttgcttcta ttactaatct
     1441 atcatatgaa atatgaacgt aagaagtaag aaagtagttg tgacagaagt ggtcagcttc
     1501 gagaaataag aaggaattta cgaaaattgc ttcttaaatt tttgtgaaat ttcggcttat
     1561 ttcttggaga taaaagcttc tgtcccagcc tcttttctat ttccccggaa acaaccgctg
     1621 ataataggca tctgcttttt tataggaaaa aatccaaata ccagatacaa aaaaacgtcc
     1681 gaataacccg atcaacggta caaaaaagac accggcaatc aagcataagt taatcatcat
     1741 ggagataaga cttcgacggt cctttgacaa aactctcacg acatgggaat ctggtttatt
     1801 tgctttcatg atttgcctca tcaagatacc aaaagaaaaa ctccacatcg tgtatactaa
     1861 gacataagcc atctcaggcc aaaaagcttc agggttacgc ccgacccaac tagtaacaga
     1921 aggacataaa gtcagccaaa agagcaaaaa gatattcgac cagaccactc ggtaattaat
     1981 tcgttctgcc atctgaaata tacgatgatg gttcacccag atcaccgcaa caaatataaa
     2041 gctgatggta taagctaata atgtatttcg gatgacccat aaagaatgaa agctagatgt
     2101 attagggatc ttgatctcta atacaataac tctcaaaaca atggctacca ctccatcagt
     2161 aaatgcttct aatctcgact ttgtcacgat gattcctcct cagatgattc agcgctttta
     2221 ttttatcact tacgttatta aataaccatc gttttatata gggaattttc tgaaaattgt
     2281 tgcatatttt tcgaaatttt gttactttta aaaaaaccgt aaaggagtta tgtatgacaa
     2341 aaaaattatc atttaaagac tatcttttta taggctccat gcttttcggt ctattttttg
     2401 gtgctggaaa tcttatcttt cccgttcatt tgggtcaaga agcaggcgct gctacctttt
     2461 gggcgaatct aggtttcttg gttaccggta tcggtcttcc cttcttaggg gtgattgcca
     2521 ttggtgtatc gaaaagctca ggtgtatatg aattagcaaa acggataaat caaacatatg
     2581 cgatggtttt taccatcttg ctttacttag ttatcggacc gttttttgct ttacctcgcc
     2641 ttgcaaccac atctttcgag attggtttag caccatttat cgatcctagc agacagacgc
     2701 tatatctagc aattttcagt attgtatttt ttgtgctggc ttggtggttt tcaagaaaac
     2761 cgacaaagat attagattat gttggaaagt tcctgaatcc tgcatttttg gttttattag
     2821 gtgctttgct tctccttgct tttttgaatc cactaggttc tgtaaatcaa gcgcctattc
     2881 agccaaatta tcaagagcat gcttttttca cagggtttac tcaaggatat aacacattag
     2941 atgcgcttgc tgctttagca tttgggatca tcattgtcac aacaatccaa aacatgggtg
     3001 taacaaaacc tgctgaaatc gctaaagaca cgatcaaatc tggtgcgatc agcattatat
     3061 tgatgggaat catttatact ttgctcgctt atttaggtgc tatgagctta ggtagctttg
     3121 ctctaagtga aaatggcggg atcacgctgg ctcagattgc tgatcattat ttaggtactt
     3181 atggcagtat cttattagca tttatcgtta tattagcttg cttgaagacc ggtattggtt
     3241 tgattacagc ttttgcagaa acattcacag atttgtttcc gcaaagaaat tacgcattct
     3301 ttgttgcact tgctagtatc cttccttgtt tagcggcaaa cgtaggcttg acgaatatca
     3361 ttcaattttc gttgcctgtt ttgatgttca tctatccatt agcaatgaca ctgattttac
     3421 tagtgttagt tggtcctttg tttaagcagc gtacggctgt ttatcgaatg actacttatt
     3481 ttacattgat tgcttcgatt tttgatgggt tgaacgcttg cccagaaact atcaaacaaa
     3541 cacctattgt tcaaaatctt ttatatgttg cggaaagcta cttgccgttt ttcaaattgg
     3601 ggatggggtg gatcgtacca gcagttatcg gttttgttat cggactgatt tggagtttcg
     3661 ctaagaaaga agaagtagca gattgataaa agaggttgtg aaagagacgt tcagccttga
     3721 gcaataaaaa gtaattttga aaaatggctt ctcacatttt tgcataaatt tgacttaata
     3781 ccgagaaact gacttttgaa caccgtttat tgagaggtcg tgaaaaagcc gcttagtttc
     3841 aagtattaag aaatattttg tgaaaatggc ttctcacatt tttgcataaa tttgacttaa
     3901 taccgagaaa ctggcttttg aacaccgttt attgagcgtc tataaactga cctgagtatt
     3961 agagaaacaa aacggcaaag ta
//
LOCUS       DAZUMV010000176         3968 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000176 DAZUMV010000000
VERSION     DAZUMV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.183"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1251
                     /locus_tag="ACG8FK_002668"
     CDS             82..1251
                     /locus_tag="ACG8FK_002668"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5098217.1"
                     /translation="MKKVTILIPGMYYGGMERVAFITRQILLKNNYNVSFVTLFDNDA
                     DYQPNFEYLSLKCDIKPSKFGKVINIFKRVYKMKSVKKKLKTDITISFGQSCNLVNVL
                     SKRDDKVYVGIRSYDWLFQYIFHYYVDKFVYKKADAVVSVSQLIKRDAENIFNFNKDK
                     SYFLYNPYDLELINNKSKEKITEIEVPTDKKIIVTVGRLEDQKGLYHLIKAISMLKNK
                     DNVVVYILGHGTKKELLENLIKAYQLQNVIFLLGGQSNPYKYMSRADLYVMPSITEGF
                     PNALVEAMSTETAVLCADCKSGPREILSNRTLDTICENIEYAESGILVCPMTDSRNYS
                     PQYEECDKNLANAIEELIENDNLRNKYAKAAKKRAELYSYDAFEHNLLQIMKTFN"
     gene            1289..2380
                     /locus_tag="ACG8FK_002669"
     CDS             1289..2380
                     /locus_tag="ACG8FK_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5098218.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(2448..3926)
                     /locus_tag="ACG8FK_002670"
     CDS             complement(2448..3926)
                     /locus_tag="ACG8FK_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFS5098219.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
BASE COUNT         1336 a          575 c          709 g         1348 t
ORIGIN      
        1 cagaaataat taatatggtt agaatgttta ctggtaaagg atattaagag agatttttat
       61 ggcaaggttg tgattaggaa aatgaaaaaa gtaacaattt taattcctgg aatgtattat
      121 ggtggaatgg aaagagtagc ttttataacg cgtcaaatac ttttgaaaaa taattataat
      181 gtatcatttg taactctttt tgataacgat gcagattatc agccaaattt tgaatattta
      241 tcattaaaat gtgatataaa gccatcaaaa tttggtaaag taattaacat ttttaaaaga
      301 gtttataaaa tgaaaagtgt caaaaaaaaa ttgaaaacgg atataactat atcttttgga
      361 caaagttgta atttagtaaa tgttttatca aagagagatg ataaagtata tgttggaatc
      421 cggtcctatg actggctatt tcaatatata ttccattact atgtagataa atttgtatat
      481 aagaaagcag atgctgttgt gtctgtcagt cagttaatta aacgtgatgc tgaaaatatt
      541 ttcaatttta ataaggataa aagctatttt ttatataatc catatgattt agagttgata
      601 aataataaga gtaaagaaaa aattacagaa atagaggttc caacagacaa aaaaattata
      661 gtgacggttg gtcgattaga agatcaaaaa ggtttatatc atctaattaa agctatatct
      721 atgttaaaga acaaagataa tgttgttgtg tacatcttag gacatgggac aaaaaaagaa
      781 ttattagaaa atttaataaa agcatatcaa ctacaaaacg taatattttt gctaggtggc
      841 caatcaaatc cttataaata catgtcacgt gcagatttat atgtcatgcc atctataact
      901 gaaggttttc ctaatgcatt agttgaagca atgtctacag aaactgctgt cttatgtgca
      961 gattgcaaat ctgggccaag agaaatatta tcgaatagaa cgctagacac gatttgtgaa
     1021 aatatagaat atgctgaatc aggcatatta gtatgtccaa tgacagacag tagaaattat
     1081 tcgccccaat atgaagaatg tgataaaaat ttagcgaatg ctatagaaga attgattgaa
     1141 aacgataatt tgaggaataa gtatgcaaaa gcagcaaaaa agagagcaga actatatagt
     1201 tatgatgcct ttgaacataa cttgttgcaa attatgaaga catttaatta attcatatga
     1261 tgaaataaaa ataagggagt ataactagat gctgaaaaaa aatacatctt tacagaataa
     1321 aacaatatta ataactggcg cagccggttt tattggatca aatttggttc ttgagttatt
     1381 acgtactcag tcatcaataa aaatcattgg cattgacaat atgaatgatt actatgatgt
     1441 ctctattaag aactatagat tatcagagat agaaacggag gcaaagaacc atccaggatc
     1501 tacttggaat tttatcaaag gttctattgc tgataaagca acaattgatt tcctctttga
     1561 taattataaa ccaagtatcg tggtgaattt agcagctcag gcaggagtcc gttattcaat
     1621 tactaatcca gatatgtata tcgaatctaa cataatagga ttctataata ttcttgaagc
     1681 ttgtcgtcat tcatatgata atggagaatc tggtgtggaa catttagtgt atgcctcatc
     1741 gtcttccgtt tatggtacta ataagaaaat tccttattct acagaggata aagtagacaa
     1801 tcaagtatcg ttgtatgcag caactaagaa aagtaatgaa tcaatggcac atgcatattc
     1861 taaactttac aatattccgt ctacagggct acgtttcttt acagtatatg gtcctgctgg
     1921 tcgtccagat atggcttatt ttggttttac tgataaactt cgtgctggtg ataatatcca
     1981 aatattcaat tatgggaatt gcaagcgtga ctttacctat atagatgata ttgttgaagg
     2041 agtaaagagg gttatgcagt gcgctccaga gaaaattgat ggggaagatg ggctaccaat
     2101 cccaccatat catatttata atattggtaa caatacgccg gaaaacctac tagattttgt
     2161 agatattcta caacaagagc taattgcagc aggggtgcta ccagaagatt acgactttga
     2221 atctcatacc gaactagttc caatgcaacc aggagatgtg ccagttactt atgcagatac
     2281 tagtgcgtta gaacgagatt ttgaatttaa accaagtaca agtttgcgtg acgggcttcg
     2341 taaatttgcg cagtggtata agtcttttta tgaagtataa ttgtattttt ttacttataa
     2401 aagtttatag aaacacctca gacactacaa atctgaggtg ttatttttta atctcgttga
     2461 aataaatctc tggtatatac tttttctttt acatcatcaa gattactatc gtaacgatta
     2521 gcaataatac tgtgactgat atttttaaaa tcatctaaat tatttattac ttttgaacca
     2581 aaaaatgtag tgttattttc aagtgttggt tcataaatta caatagttgc cccctttgct
     2641 ttaattcgct tcataactcc ttgaatagat gactgacgga agttgtctga attactcttc
     2701 attgtaagac ggtatacacc tatgactacc tctttttctt tttctgcatt ccacgaactg
     2761 ttagcctcat acgtgccagc aagttctaac actcgatctg ctataaagtc ttttcttgta
     2821 cgattactat cgacaattgc acttatcata ttctgaggaa catcgttata gttagtgaga
     2881 agttgttttg tatctttcgg taaacaatag cctccatatc caaaagatgg attattataa
     2941 tgtgtgccaa ttcttggatc taagcaaacc ccgtgaatga tttcttgtgt atttagtcct
     3001 ttcatttctg cataagtatc cagctcattg aagtaagata ctcgaagtgc aaggtaagta
     3061 ttagcgaata atttaactgc ttcagcttct gtgaatccca tgaaaagagt ttcgatatct
     3121 tttttgattg ctccttcttg gagtaaatct gcaaaagtgt gagaggcttc tcgtaaagtt
     3181 ttgtcagttt ggtccgtgcc gacaataatt cgcgaaggat atagattgtc atataatgcc
     3241 ttagattctc tgagaaattc tgggctgaat agaatatttt tagtattgta ttttttacga
     3301 atactttcgg tatagcctac gggtaccgtc gacttgatga ccattattgc ctttggattg
     3361 acttctaaca ccaatttgat tactgactct acagcagaag tatcaaaata attttgttta
     3421 ctatcataat ttgttggagc agcaatgaca acaaaatcgg cttctctata ggcggctgca
     3481 ccatcagtcg ttgcaattaa attaagattt ttttcagcta gatatttttc aatgtaatcg
     3541 tcttgtattg gagatttctt attgttaatt tgttttactt tttcaggaag aatgtctact
     3601 gctgttacct gattgtgctg ggcaagaaga gtagcaatgg aaagacctac gtaaccagta
     3661 ccggctacag caatattctt cgactgatgt tttgttctgt ttgatttatt tgattcattt
     3721 ataaatagat ctgttggttc aaaatttaga acttctgcta atgattctaa ttgttgtatt
     3781 gacgggataa aatcgagaga ttctactcga ctaattaagg cacggttgat accagtagct
     3841 actgcaagtt gttgttgtgt cattttcaga gattttctag aatcaataat gatttgagct
     3901 aaacgttttt gagataactt tttcatgatg cactcctttt tttgttgcta ataataacaa
     3961 acaatttt
//
LOCUS       DAZUMV010000177         3930 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000177 DAZUMV010000000
VERSION     DAZUMV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.184"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..635)
                     /locus_tag="ACG8FK_002671"
     CDS             complement(93..635)
                     /locus_tag="ACG8FK_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS5098220.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            770..1159
                     /locus_tag="ACG8FK_002672"
     CDS             770..1159
                     /locus_tag="ACG8FK_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098221.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1163..1927
                     /locus_tag="ACG8FK_002673"
     CDS             1163..1927
                     /locus_tag="ACG8FK_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5098222.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2056..3003
                     /locus_tag="ACG8FK_002674"
     CDS             2056..3003
                     /locus_tag="ACG8FK_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5098223.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3133..3873
                     /locus_tag="ACG8FK_002675"
     CDS             3133..3873
                     /locus_tag="ACG8FK_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098224.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1304 a          669 c          802 g         1155 t
ORIGIN      
        1 atatgtcgat gttttttaat caaaataata aataaaaact gtctaacatt taaaatttaa
       61 tttagatatt agacagtttt taagtttgtg agttattttc cttgtcgttt tctcactttt
      121 ttagtccaga aaccacctga aatatatttt ggctcataag aaatgataaa agcacgctct
      181 tcgatttctt tgatcaggcg atacagttca cgttcgcttt ttctaggaga aagtatttcg
      241 agaactagcc gctcaccttc cagccccatt gcagaagtct gtgtcacacc ataaccgtgt
      301 tctctcagta tacccggcaa acttttatct tctgcgctat ttgctggcaa aatcgttgtg
      361 accatgatgt atcctaatgc caatttgtct tcaatcaaaa ttccgacact gatacctact
      421 gcgtaaccta acgcatagac aaataaattc agtggattat ctaaacgatc taaaacaagg
      481 gataagccga cgatataaat cgtgatctcg accatactga caagtggagc aatcagtcga
      541 tagcctttca ttgtcaacat gaaacgtaac gtgttcagag tgatataagc aaaattaatg
      601 aggaagatca tccccaacat ttttggatca atcatttgta aaactccttt catttatata
      661 acaacaatat caaaaaaaat cgtaagtgaa gaaaaattga aactttatac atacaaaaaa
      721 atgataaaat gattataata gaattataaa gataggttag gtgattaaaa tgaaaacggt
      781 caatcgtgtg ttttgtgcca gccacgtgct aagtctcctt ctcagtatat acttaggtgt
      841 ctattcgatt caacagccaa ttgaggtaga gaacattgtt tttcagatgt cgctgacaga
      901 tggtttaatt ttaagcgttt ttttcttttt gatactgttt attggtaata ttttcggtag
      961 cgttgccagt tttttgaatt tttctattta tccactcctt tcgctagcat taggagtggt
     1021 gggactttgt tctctgtgct tatttcccga gccattcagt cctgcattta tattatttgg
     1081 tacactgaac ctgttccaga caactgttgg agcatggttg ctatggcgat caggaaattt
     1141 aatgaaaata ggtgagtaac aattgaatcc aattatattg aaacgttctg tccatcatat
     1201 tccagtgtta gaagttgttc tgaaagaatt aagagaggaa cgtattcccg ttgtcatcta
     1261 ttatcacgga tggcagacaa gtaaggagtt agttttgacg caaggaagaa aaattgcgca
     1321 gcagggcatt cgtgtgatcc tgccagatgc gatgaatcat ggggagcgca aacagccggt
     1381 ttcttctatt ccatcctata cattttggga tagtatctac ggaaatctgt ttgaatttga
     1441 cacacttatc aagcatttag aaaaaagagg tcttctacat gatcagcttg cagttggcgg
     1501 ggtttcgatg gggggaatga caacatgcgc cttgttggct aaacatcctc atatagtagc
     1561 gggcgtgtgt ttgatgggtt cccctgcacc tttggaatat ggaaaagaaa tcgtaaggcg
     1621 cgcatcggaa tataattatg tcgtaccaaa agactatttt gatttgattt catgggttaa
     1681 gagctatgat ttgtcattgc agccggaaaa gttagctgga cgtcctctgt tcttttggca
     1741 tggacaagct gacgaaaaaa tcccttatat acaaacggaa cgatttgtag aaaaaaatcg
     1801 gcataagtct tacggaaaag aaattgtttt tcgttcctca aaaacacagc gacatatggt
     1861 acaaatacca ctaatggaag aagcttccac ttttttagca aaaaatttga taaaaaagta
     1921 taaataattg aaacggaaaa tcactcatga cgatagagga ttttccgttt tttattttgg
     1981 gctataataa actctgttag gaaaatttgg tataattgat agtaagaatg acaacatgtt
     2041 agaaaggaag agagcatgcc cactttagca gatgttgcaa aacgtgccaa tgtttcaaaa
     2101 atgacggtct ctcgagtcat caatcatccg gaacaggtga ctgacgaatt gaaagagtta
     2161 gttttctcag caatggctga actggattac cgtccaaata ttgctgcaaa agcacttgtc
     2221 acgaatcggt cgcagatcat caaattattt atactggaag aaatcgacac aactgagccc
     2281 tattacatga atttgctgat gggaatcgct aaacggatcg gtaaagcgca ttattctctc
     2341 caattagtta caaatgatgc cttcgacgtc ggttcttgtg atggatacat catcacaggt
     2401 gtcagaaaaa aagacttcga ttggatcgac cgtttggaaa aaccagttgt cttgtttggg
     2461 gaaaattctg aaggatatga ttatgtagac agcgataatg aatatggtac agcaaaagca
     2521 acgcaatatg ccttagaaag agattatcaa acgattattt atatcggtat cgaagtggac
     2581 gagccttttg aaatttcaag ggaaactggt tatttatctg tgatgaagga agttgatctt
     2641 cctagtaaaa tcttgcggtt tccaaatcat tcgacagaag tagaaagata tatgacagaa
     2701 aactgggcag attttacaga taaaacttgt tttgtatgta gttcagatcg gctagcacta
     2761 ggagcagtaa gagggattat aaatgctgga ggacacttgc ccgaagaatt tggtgtgatt
     2821 ggatttgatg gggtattttt agaccaaatc agttcaccta aattgacaac gatcaagcaa
     2881 gatattatac gtatgggtga agtatgtgga gaaatgttgt taaaaaaaat agaagaaaaa
     2941 ggggcgagtc aaggttatcg tcgttttttt cctgaactag ttatcagaga gacaacgaat
     3001 taagtcttat tactatatat atatttatga aattcatcgc tattttctta aaataatcag
     3061 cgcattgcta ctgaaaacaa tgaatctgtg ctattattga gaaaagttcc atttttgaga
     3121 aggatggttt gaatgaaaga aatcagacga aaacatttcc gttttcctgc gtacgatgat
     3181 gagttaggcg taaaattaac caacgacaag aagcgagtat tatttgacgg acaagaagat
     3241 cttttgactg aacagaatca gtccccgttt gaacagatga aagaatttca tttcacgaac
     3301 acgaacaccg agaaaatccg tcgcaagcgt cgtgcaacaa atggattgaa gtcagctccg
     3361 cgtaaggaaa atttggaaaa gcataaagga aaattaccgg attataccgg ccattaccaa
     3421 tcagaaatca catcgacagg acgaaaaaaa ctttttggtg attcctctcg ttctgtcaga
     3481 caaagtgaaa caaggacaga gtccaagcat actacacgaa acgcatctgt tgacacgaag
     3541 gtgcgggagc gttcttattt tgttcctaaa tatgtacccg catcgattat cccagaccct
     3601 agagaaagca aaatttctga ggatcattta atcgaatcga tgaaaaaaac ggatgactct
     3661 tatctgttgt tcgatacaga accgacgccg tatcaagaaa aaagaaatgg tgagccgtct
     3721 gtacgaacat tcaatcgaac aaaaacgtta gaggaaacaa agcagtcaga agaaaaaaaa
     3781 tcgaaaaaaa gccatggtat cttagaacgc tcattacaag gattgatcga tgatcaagga
     3841 aactctttgg gagaaaatag ctattttaaa taagtgatat acaaatttat attcggctac
     3901 agttggaaag gaaacgctgc ggcgtttcct
//
LOCUS       DAZUMV010000178         3785 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000178 DAZUMV010000000
VERSION     DAZUMV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.185"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..168
                     /locus_tag="ACG8FK_002676"
                     /pseudo
     CDS             1..168
                     /locus_tag="ACG8FK_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            406..642
                     /locus_tag="ACG8FK_002677"
     CDS             406..642
                     /locus_tag="ACG8FK_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098225.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="ACG8FK_002678"
     CDS             649..2601
                     /locus_tag="ACG8FK_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5098226.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="ACG8FK_002679"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="ACG8FK_002679"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5098227.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3445..3785
                     /locus_tag="ACG8FK_002680"
                     /pseudo
     CDS             3445..3785
                     /locus_tag="ACG8FK_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DAZUMV010000179         3721 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000179 DAZUMV010000000
VERSION     DAZUMV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.186"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..1915
                     /locus_tag="ACG8FK_002681"
     CDS             200..1915
                     /locus_tag="ACG8FK_002681"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5098228.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1918..3681
                     /locus_tag="ACG8FK_002682"
     CDS             1918..3681
                     /locus_tag="ACG8FK_002682"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5098229.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         1108 a          730 c          740 g         1143 t
ORIGIN      
        1 cctatgaatg aagagactgg catgttttct cattttcttt tttttaaaaa aaatagtcta
       61 ttcaatttct aaagcttctt cataataaaa ctttcattta tgaaaaggct tgttctcttt
      121 tcattctaaa tttcctaagc tacaatgtgg tagtacttgt tttttcaatt gtggacaaat
      181 aaataatagg aggaatcata tgattcagtt attacgttat gcaaaagact atcgtaagca
      241 aatcattttg ggcccgttct tcaaatttct tgaagctgtc ttcgaacttg tcctgccttt
      301 aatgatggct tccttgatcg ataatggact aaaaatgaat gaccgaggaa aaatcattga
      361 aatgggctta tggatggtcg ctatgtctgt gattggtctg atttgtgcta tcatttgcca
      421 atactatgct tcagtagctt cacaaggatt tggtacagag ttacgtaatc agctaatcaa
      481 aaaaatcaat actttctctc acaacgagtt gaatcatttt ggaacagata ccttgatcac
      541 ccgaatgaca aacgacatca atcagcttca gctagcttta gcaatggtca ttcgtttatt
      601 gatcagagca ccgtttttaa gtatcgggtc agtggtcatg gcttttgtga tcgactggga
      661 agtaggtctg tttttcttag cattattgcc tattttctca atcattttat tttttatcat
      721 caaaaaaacc gttcctctct accaaaaagt gcaagaaaaa ctagatcagc tcaatgaaca
      781 cgttagtcag aatcttagcg gtgttcgtgt catccgcgca tttgcgcaga caaaaaaaga
      841 aacagagaag ttcgataaaa gcacaaatga tctagcgaac aattatttac gtgtttccaa
      901 tatctctgcc ctattatcac ctgctacaac cttgatcatg aatgtcggga tcatctgttt
      961 gctgacagtt ggcgggatca aagtaaatat tggtagcttg caacaaggac aagttcttgc
     1021 tttgatcaat tatatgaatc aaatgctttt ggcactgatc gttgtttcaa acctcgtcgt
     1081 gatcttcaca cgtgccgaag cttcaggaaa tcgggtgaaa gaggtgctag acacagaaaa
     1141 ttcgattctt gaagctgagg tcagtactac tcccgatttt tcttctgaag caattatcgc
     1201 ttttgaccat gttgacttcc gttatactcc tgattctggt ctttctctcc aaaatattac
     1261 atttcagctg aagcgaaata cagtccttgg aatcattggt ccaaccggaa gcgggaaaac
     1321 gactttgaca caattgattc cgcgtttcta tgatgtcagt tccggtacag ttataatgga
     1381 cggaatggat gttcgttctt ggcagctgga tcatctccgt cagcaaattg ctcttgttcc
     1441 ccaaacttct gtactattta ccgggactat tcgtgaaaac cttcaatggg gtaagaaaga
     1501 cgccacagaa aaagaatgtt gggaagctct gaaaatcgct caggcagaag aatttgtccg
     1561 tcagcttcca aatggtttag atacaaaagt catggaaaat gggaaaaatt tctctggcgg
     1621 acaaaaacag cgactgacga ttgcaagagc tttgattgcc aagccagcat tactgatttt
     1681 agatgactct ttaagtgcgt tggattatca aacagacttg gatttgcgta aggcgctaca
     1741 gacttatctt ggcagtacag tgattattat ctcccaacgt gtccgctcta ttcaagaagc
     1801 acatcatatc ttagtgatgg atcaaggtaa gattgctgct cagggtaccc atgaagaatt
     1861 acttgctcaa tcagcagaat atcaggaaat cgtcgcatca caggaggaag aatagttatg
     1921 aaagtaaaaa aattatcctt gcagacgctc cgccgattca ttccttacct tactgcctat
     1981 gcaaaagaca tttggctagc tgtgatacta ggaatcatca atgggattgc aacagttgtg
     2041 atgacgttac aaattggtca aagtatcgat caaatgattg gagaaggaca ggtccgcttc
     2101 aatcgactgt tccatttact tattctattc ggtggtatcg ttttaatcaa cgtagtcagt
     2161 caatggctca tccaagtgtt aagcaatcgg ttagcttact tatccgttgc aaaattaaga
     2221 aaagatgcat ttgctcatct gaatcgattg ccacttcgtt attatgatca aaactcccac
     2281 ggaaatatcg tcagtcgatt tacaaatgat atggacaata tttccattgc aatttctgcg
     2341 gccttcaatc aattattttc tggagtggca atcatcattc tttcgtttat ttttatgatg
     2401 cgtctaagtc cacttctgac attggtcgtt ctttgttcta caccaattat tttccttgtg
     2461 agttggatcg ttgctcgtgc ttcacaaaca aatttcgata gacagcaaag gattattggg
     2521 gatatctcgg gctttgtgac cgaacgaatc ggtaaccaga aaatcgtgaa agcttttcag
     2581 caagaagaag ctagtcaaca gcaatttgaa ctgctaaatg ctgatctata tgaaaaagga
     2641 caacgggcac aattttcttc ttccttgaca aacccgtctt ctcgttttgt agatcacctt
     2701 gcttatttat cgattggttt agttggcggc ctattggcat tgaagagtgg ttcgtcaatc
     2761 actgttggtg tgatttccag ctttacgatc tactcaagtc agttttctaa accatttatc
     2821 gaactttctg gaatcacgac acaactgcaa accgcttttt ctggtctaga acgagcattt
     2881 gatttgatcg atcagccaga agaaaagcct gatttgccag atgcctatgc cttggaaccc
     2941 aaaaatatca aaggaaaagt ctcttttgat catgtttctt tttcttatga ccccaagcag
     3001 cgtttgatcg aagatttcaa ttttactgct tatccaggtc aaaccattgc tatagtagga
     3061 aaaacagggg ccggaaaatc cacacttgtt aatttgctga tgcgttttta tgacacaaca
     3121 aaaggaacaa ttacagtcga tgaccatgac atccgctcta tccaacgtga tacattacgt
     3181 aaaagttttg gaatggtgtt acaagatact tggctatttg acagtacatt gcgagaaaat
     3241 cttcgttttg ggaatccgga agcaagtgac gcagaaatca atgatgccct aaaagcctct
     3301 tatatgtatg aatttgtaga acgtttgccg gaaaaattag atactaagat tggcggtcaa
     3361 gggttaaaga tatcagatgg acaacgccaa ctgttgacga ttgctcgaac gatgatcagt
     3421 aatcccccta tgttgatctt agatgaagct accagctcag tcgatacatt gacagagaaa
     3481 aaaatccaac aagccttctt agcaatgatg gaaggaaaaa ccagttttgt gattgcccat
     3541 cgcctgtcca caatcaaaaa tgcggaccag atattagtga tggatcaggg agctattgtc
     3601 gaaatgggca cccatgatga gctgcttcaa aaagatggtt attaccgacg gctctatcaa
     3661 gcacaattca cagcacaata aaaacaagca gtcttctggg atgggaagac tgcttgtttt
     3721 t
//
LOCUS       DAZUMV010000180         3713 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000180 DAZUMV010000000
VERSION     DAZUMV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.187"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            254..853
                     /locus_tag="ACG8FK_002683"
     CDS             254..853
                     /locus_tag="ACG8FK_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5098230.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            902..1618
                     /locus_tag="ACG8FK_002684"
     CDS             902..1618
                     /locus_tag="ACG8FK_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFS5098231.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            1632..2411
                     /locus_tag="ACG8FK_002685"
     CDS             1632..2411
                     /locus_tag="ACG8FK_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS5098232.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            2494..2850
                     /locus_tag="ACG8FK_002686"
     CDS             2494..2850
                     /locus_tag="ACG8FK_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5098233.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            2840..3106
                     /locus_tag="ACG8FK_002687"
     CDS             2840..3106
                     /locus_tag="ACG8FK_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5098234.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            3265..3543
                     /locus_tag="ACG8FK_002688"
     CDS             3265..3543
                     /locus_tag="ACG8FK_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098235.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(3540..>3713)
                     /locus_tag="ACG8FK_002689"
     CDS             complement(3540..>3713)
                     /locus_tag="ACG8FK_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5098236.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT         1370 a          536 c          728 g         1079 t
ORIGIN      
        1 agaataactt aaaagtactg acaaattttt taaaggggca atattaatgt tctgagataa
       61 tcaaatctta ccgaaaatat caaaagtcca atgtgacaca attggatttt gtcacattcg
      121 ttaaaatcgc tccatcctta gaggctctaa taggaaatca cttttctttt agcgcctttt
      181 ttctatagct tgtttaaatg tgacttatta tctgttataa tcgtattaag taacatttga
      241 gaggtgtcta aaaatgaaaa gcgtggaacc gattcgcgac aaaaaaaaga tcgatgccat
      301 gaaggccatt ttggcttccg gaaaatatgg gcaacgaaat ttggtgcttt tttcgatcgg
      361 gatcaataca gcctatcgaa tctccgattt aagacagtta aaattaaggg atgtgctaga
      421 aatttcccgt gggcgggtga tcgtcaaaga acgtctggcc atgaaggaac aaaaaacggc
      481 caaacacaat tcggtcttca tttcgaacaa attgcgaaaa gtgattcttg attatgttca
      541 atccgaattt cctgagcagt tgcaagcaca agacttcagc aagtatttgt ttccaagtcg
      601 aaaaggagca gatacgcctt tgacccgaca aagcctttgg cggatcattc atgaagcagg
      661 aactgcagtt gggttaaaag aaattggtcc ccattcgatg cgtaagacct ttggctattt
      721 tttatacaag caaggaacca aaacagagat cattcagtcg ctgctaaacc attcttcaca
      781 acgggagacc ttacgctata ttggaatcac ccaggaagac aaagatactg cagtaaagag
      841 tttggattta tgacagaata atgccctata taatagaagg aaaaattcaa gaggtgagaa
      901 aatgaacgaa gaaaagcagc ctaactttcc tgataaatat cacttatctc gcaaagagtc
      961 tgtgtattta ttgaagaaaa acattgtaga actggtgtat aatgcgggga aatttgaagg
     1021 gttaaataca acgttgttac aaacagaagg gattattaag tataatcggg ccaacaacgt
     1081 ggctgtagat gatgtcctga ccgtcgttaa cttaaaaaga ggctttgaaa tgcttttaaa
     1141 tgacgtacag gagcccctgt tagagacaag taagcggatt aatcggatcg ttgcagcaga
     1201 ggatgcgctt tttcccggtg aaatacgaac cggtggggta gaattatcga cgattcaagg
     1261 aagatatgtg ccaccaatgt taactgaaga cgaggtaaac aatcaatatg gtgagataat
     1321 gaatcaagag atatcagata cggaaaaagc cctgcgactg tttctattca tttcgaaaaa
     1381 tcaaatattc tggaatggta atgtgggtac agccttgttg attgcgaata agattatgtt
     1441 tagcaacggt ttaggactat tatcaatacc cgaaaatgta tttatgaaat ttaacgaact
     1501 tttatcaaag tattgcaaca gtaatctatt agtcaatgag tcaaatatta tatcattttt
     1561 atatgaacag tgtatctttg gtattaatta tttgaaaata atccaacata caatttaaga
     1621 ggtataagta aatgatgact atagacgaag caaaacaatt agcaacttat ttttcagttg
     1681 gttcagcggg atatatttta gactttacta atgtaacgtg gaacatattt acaaagaaaa
     1741 tcgtagggat tgaaatacaa aatcattatg ggggctcaaa gggagtatcc tttcaaaagt
     1801 ttctgcttga cgatcaattt gatgattcgt taaagaatcg gttaatccaa tctttaaaag
     1861 gtattatgga tcgagataaa aaatatgaat ctactgaaat gaatcccata gttcagttgc
     1921 tttttgagga agtatttaaa gaagatactt caactgcatt aagaaaaatg aaattttcaa
     1981 gcgagattga tttactttat attcggcagc tacccattag gattcaaaca gatattaaaa
     2041 acaaggattt tgactctgtt ttgacaaaag caaatacttt aatagatgaa gttctaaaat
     2101 ttattattga aaataccgaa ggaccgagtg ttaatattaa tttatttaag tcaaaagaat
     2161 taagaaaaga agtatttggc cgcctcaata ttaaaactga aaaagatatg gataatagaa
     2221 tcaaagccct tgttggagca ctcaatacat tatcagataa aatactcgaa atgagaaata
     2281 gtcaaggaga tgctcatgca catggctcaa atagaatagt aattaaggaa gaagaagcaa
     2341 ttcttgttgc aaattcttct atggttttgt gcgagtatgt gtttactaag tataaaggat
     2401 taaatagata ataaaatgca tattttatag tatacaattc gaatttttat gttatatact
     2461 ataatataaa atcaatatat gatataattg ggtatggaaa aaatatcttt tgaaacattt
     2521 aaaagaccca atggtcatga tgaattttta gaatggttgg aaacattacc aaaaaaagat
     2581 agtgcgaagc ttttacgtac aatagaagaa acagaaaaga atgggatgct agtagctcaa
     2641 cggttgaaat gggtgaaaaa attagacact aatttgtacg aattaagaag taaggtagga
     2701 tcaaacatcc aaagagccat ctatttccat gtggacaatg gaaggtatgt cattactcac
     2761 ggatttacta aaaaaacgga gaaaacacca ataactgaac tcaagcatgc aaaagaactt
     2821 agagaggagt ggttcacaaa tgatgattaa aaaattgatt gaagaaaaga aaaaagataa
     2881 agattttgca gaagcatatg accgtgaagg aagaaaactc gaaactgcgg ttgctcttta
     2941 tcatgctcgg gaggaagcag ggctaactca agcggagctt gcacaaaaag cacatacaac
     3001 tcaatctact attgctcgaa ttgagagagg ggataacgta tctttcgata aacttgatga
     3061 tatcgccagg gcattaggta aaaagctggt agtagaattt gcttaaaagc gagttgaaaa
     3121 tatatctata ataggctcct aactaatcaa ttagtttagt gggccttttt ttactagata
     3181 gataaaggtt gtactatagt gtaaaagaca ataaatttta tactaaaatt actttaaaga
     3241 gaactagaga gaaaggtaca ataaatgaaa accataaatc ttataaaagt atttatagca
     3301 tcaccaggtg atatagcagc aaaaaggaat gaagtagaag aaaccatata tagatggaat
     3361 atagaaaata cagataatac aaaagtgatt ttgatgccta tacgatggga aactaatgcg
     3421 attcctactt atgtaaataa tagctctggg caaagtgtta ttaataaaca aattgtagaa
     3481 aatagggttc tgctgcaaag ttttaaaaat aaaatcagtt tttgtattat ttcttcattt
     3541 taagctggaa ttcccaataa tatcctgatt tcagtgcaga ctgaaaagcc gaagagagtg
     3601 ccttcttttc ggcttttctt gtagattcct cgaatggttt ccatcccttt aattgtggtg
     3661 gaagctgtac gtagactttg atacaattta tgtcgacgtt ttatcgatcg atg
//
LOCUS       DAZUMV010000181         3699 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000181 DAZUMV010000000
VERSION     DAZUMV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.188"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..437
                     /locus_tag="ACG8FK_002690"
     CDS             123..437
                     /locus_tag="ACG8FK_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5098237.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            453..839
                     /locus_tag="ACG8FK_002691"
     CDS             453..839
                     /locus_tag="ACG8FK_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5098238.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            868..2253
                     /locus_tag="ACG8FK_002692"
     CDS             868..2253
                     /locus_tag="ACG8FK_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5098239.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2256..2409
                     /locus_tag="ACG8FK_002693"
                     /pseudo
     CDS             2256..2409
                     /locus_tag="ACG8FK_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            2432..3637
                     /gene="mobT"
                     /locus_tag="ACG8FK_002694"
     CDS             2432..3637
                     /gene="mobT"
                     /locus_tag="ACG8FK_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5098240.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         1214 a          680 c          809 g          996 t
ORIGIN      
        1 cccgattttg aaaggaagtg aacttatgaa aacaaaaaat caagaatcaa aaggtcgttc
       61 cccactcttt aagaccatca aacattcatt cagccaataa aaaagaaagg ataggtaaaa
      121 atatggaact taaatttgtg attcccaaca tggaaaaaac attcggcaat ttagaatttg
      181 ctggcgagga taaagtcgtt cagcgaagaa tcaacggacg gctaactgtc ttatcaagaa
      241 gctataatct ctattctgat gttcaaagag cagatgatat tgtggtggtg cttcctgctg
      301 aagctggcga aaaacatttc ggctttgagg aacgtgtgaa gttagtcaat ccacgtatta
      361 ccgcagaggg ctacaaaatc ggcactcgtg gttttacaaa ttacctttta catgctgacg
      421 acatgataaa agaataaaga aagagaggaa aaatgatgag attagcaaat ggcattgtat
      481 tagataaaga cacgactttt ggagaattga aattctctgc tctacgtcgt gaagtgagaa
      541 tccaaaatga agacgggtcg gtttcagatg aaatcaagga acgtacctat gacttaaaat
      601 ccaaaggaca aggacgcatg attcaagtaa gtattcctgc cagcgtgcct ttgaaagagt
      661 ttgattataa cgcacgggtg gaacttatca atcccattgc ggacaccgtt gctactgcca
      721 cctatcaagg agcagatgtt gactggtata tcaaggcaga cgatattgtg ctgacaaagg
      781 attctagttc attcaaagct caaccacaag caaagaaaga accgacacaa gacaaatagt
      841 cgctaggtag aaaggagact ttttcgcatg aaacagcgtg gtaaaaggat tcgcccatct
      901 ggtaaagatt tagtctttca ttttacgata gcgtcactcc tgcctgtttt cctgctggtt
      961 gtcggactgt ttcatgtgaa gacaatccag cagatcaact ggcaggattt taacctatca
     1021 caagcagata agattgacat tccctattta attatcagtt tcagtgtcgc aattcttatc
     1081 tgcttgctgg tagcgtttgt attcaaacgg gttcgctatg atacggttaa acaactttac
     1141 caccgtcaaa aactggcaaa gatgatactt gaaaacaagt ggtatgaatc tgaacaggtc
     1201 aaaacagagg gtttctttaa agatagtgct ggtcgtacaa aggaaaagat aacctacttc
     1261 cctaaaatgt attatcgact taaaaatggc ttgatacaga tacgggtgga aatcacgctg
     1321 ggaaaatatc aagaccaact cttacacttg gaaaagaaat tagagagtgg cttgtactgt
     1381 gagctgacgg ataaagagtt aaaggattcc tatgtggaat atactttgct ctatgacacc
     1441 atagccagtc gtatttctat tgatgaagta gaagctaaag atggtaaact tcgcttaatg
     1501 aaaaacgtat ggtgggaata tgataagctc cctcatatgt tgattgctgg tggtacaggt
     1561 ggcggtaaaa cttactttat actgacactg attgaagcct tgcttcatac agattcaaaa
     1621 ctgtatattc ttgacccgaa aaatgctgac cttgcggact taggttctgt gatggcaaat
     1681 gtctactata gaaaagaaga cttgctttct tgcattgaaa cattctatga agaaatgatg
     1741 aaacgtagtg aggaaatgaa gcagatgaag aactataaga ctggcaaaaa ttatgcttac
     1801 ttaggtctcc cggcacactt cttaatcttt gatgaatacg tcgctttcat ggaaatgctg
     1861 ggaacaaaag aaaacaccgc agttatgaat aagctgaaac agattgtcat gttaggtcgt
     1921 caagctggct tctttctaat actggcttgt caacgtccag acgcaaaata tttaggcgac
     1981 ggaatccgtg atcagtttaa tttcagagtg gctttaggtc gtatgtctga aatgggctat
     2041 ggcatgatgt ttggcagtga cgtacaaaag gatttcttct taaagcgaat caaaggtcgt
     2101 ggctatgttg atgtaggaac aagtgtcata tcagagtttt atactcccct tgtaccaaaa
     2161 ggatatgatt tcttggagga aattaaaaag ttatccaaca gcagacagtc cacgcaggcg
     2221 acgtgcgaag cggaagtcgc aggtgtggac tgatcttgct ggctggtgtg gcaatagcca
     2281 cgccagcact taacccctcg gtatctaaca ggggggtaca aatcgacagg aaacagtcaa
     2341 aaaaacatta gaaaatcctt tggttacaag ggatttacaa aatttcagcg tatgtcaaat
     2401 gggctttaaa agttgacata cgcctttttg attggaggga tttttactga atgaacaaac
     2461 ttggttacag catttaaaag aaaaacgctt ggcttatgga ctatctcaaa accgtttagc
     2521 tgttgcgact ggtattacaa ggcagtatct aagcgatatt gaaacaggaa aagtcaagcc
     2581 atcagaggat ttacagcagt ccctttggga agctctggaa cgcttcaatc ccgacgctcc
     2641 ccttgaaatg ctgtttgatt atgtaagaat tcgctttccg acaacagacg tacagcaggt
     2701 ggtcgaaaac atcttacaac tgaaactgtc ctattttctt catgaggact atggtttcta
     2761 ttcttattca gagcattatg ctttaggcga catattcgtc ctttgctccc atgaactgga
     2821 caaaggagtt ctggtggaat tgaaaggtcg tgggtgcaga caatttgaaa gctatcttct
     2881 ggcacaacaa agaagctggt atgagttctt tatggacgtt ttggtggctg gcggtgtgat
     2941 gaaacgcctt gaccttgcca ttaacgataa gacagggatt ttaaatatcc ctgtactcac
     3001 tgaaaagtgc caacaggaag aatgtatctc cgtcttccgc agttttaaaa gctatcgcag
     3061 tggcgaactg gtacgcaaag aggaaaagga atgtatggga aacaccctct atatcggttc
     3121 attacaaagt gaagtttatt tctgtatcta tgaaaaggac tacgagcagt acaagaaaaa
     3181 tgatattccc attgaagacg cagaagtaaa aaaccgtttt gagattcgat tgaaaaatga
     3241 gcgtgcctat tatgcagtcc gtgatttact cgtctatgac aatccagagc ataccgcctt
     3301 taaaattatc aatcggtata tccgttttgt agataaagac gattccaaac ctcgttctga
     3361 ttggaaactg aatgaagaat gggcttggtt tattgggaac aatcgtgaac gattaaaact
     3421 aaccacaaaa ccagagcctt actccttcca aaggacgctg aactggctat ctcatcaagt
     3481 tgccccgacc ttaaaggttg cgattaaact tgatgaaatc aaccagacgc aggttgtaaa
     3541 agacattctc gaccatgcga aactgacaga ccgacacaag cagattttga agcaacagtc
     3601 agtaaaagaa caggacgtga taacaacaaa aaaataactc aaatacaaat tcattgaata
     3661 tagagaggag aacattttta tgaattttgg acaaaacct
//
LOCUS       DAZUMV010000182         3695 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000182 DAZUMV010000000
VERSION     DAZUMV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.189"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..494
                     /locus_tag="ACG8FK_002695"
     CDS             <1..494
                     /locus_tag="ACG8FK_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HFS5098241.1"
                     /translation="KEKRTFLAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFS
                     MTAWAGSNMIGNFFGSVIRVDADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGV
                     VFMVLFQNTFGLIAATVIASFAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEAN
                     HHD"
     gene            487..804
                     /locus_tag="ACG8FK_002696"
     CDS             487..804
                     /locus_tag="ACG8FK_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5098242.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1048..2658
                     /locus_tag="ACG8FK_002697"
     CDS             1048..2658
                     /locus_tag="ACG8FK_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5098243.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            2815..3663
                     /locus_tag="ACG8FK_002698"
     CDS             2815..3663
                     /locus_tag="ACG8FK_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5098244.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         1084 a          649 c          752 g         1210 t
ORIGIN      
        1 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
       61 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
      121 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
      181 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
      241 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
      301 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
      361 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
      421 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
      481 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
      541 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
      601 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
      661 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
      721 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
      781 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
      841 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
      901 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
      961 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
     1021 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
     1081 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
     1141 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
     1201 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
     1261 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
     1321 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
     1381 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
     1441 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
     1501 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
     1561 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
     1621 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
     1681 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
     1741 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
     1801 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
     1861 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
     1921 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
     1981 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
     2041 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
     2101 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
     2161 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
     2221 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
     2281 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
     2341 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
     2401 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
     2461 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
     2521 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
     2581 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
     2641 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
     2701 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
     2761 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
     2821 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
     2881 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
     2941 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
     3001 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
     3061 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
     3121 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
     3181 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
     3241 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
     3301 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
     3361 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
     3421 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
     3481 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
     3541 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
     3601 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
     3661 taattccttt tattcaaaaa acataccaaa aatat
//
LOCUS       DAZUMV010000183         3631 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000183 DAZUMV010000000
VERSION     DAZUMV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.191"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8FK_002699"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8FK_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8FK_002700"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8FK_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS5098245.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8FK_002701"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8FK_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8FK_002702"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8FK_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS5098246.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8FK_002703"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8FK_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5098247.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
BASE COUNT         1182 a          622 c          612 g         1215 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt c
//
LOCUS       DAZUMV010000184         3627 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000184 DAZUMV010000000
VERSION     DAZUMV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.192"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..924)
                     /locus_tag="ACG8FK_002704"
     CDS             complement(<1..924)
                     /locus_tag="ACG8FK_002704"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5098248.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTL
                     SRLQNSKTEYDKTYLDFIKIVSIFIIPLGIGI"
     gene            complement(983..1444)
                     /locus_tag="ACG8FK_002705"
     CDS             complement(983..1444)
                     /locus_tag="ACG8FK_002705"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFS5098249.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
     gene            complement(1455..2231)
                     /locus_tag="ACG8FK_002706"
     CDS             complement(1455..2231)
                     /locus_tag="ACG8FK_002706"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS5098250.1"
                     /translation="MAFQVYLQRVYILSTGVIIGHYAEMGLNRIAYAGLMGDFSFAGL
                     YLATGCLYAFLKYVDLKNWKLLFFIIIEVFLLGAILCVSARTGLVSLFVTMTLYYLFN
                     INRISVPHLLVLIGGIITTPYILVMLMAGRSGQSLLDSSGRLENYMSSLNAFSDKYLI
                     GYGLGLNNLYTLLGVAVPHNFFIQYLLQTGIIGTLIILSGFFKFTYEELKKSGCYKWL
                     FLMIVVGAMFIPDIVSSRFLYAIVVLCMISASERTIKKES"
     gene            complement(2760..>3627)
                     /locus_tag="ACG8FK_002707"
     CDS             complement(2760..>3627)
                     /locus_tag="ACG8FK_002707"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5098251.1"
                     /translation="FSRKEIDRAVHELNPDIIHAHDFNASVRASRYKKQIILSHLHNN
                     PLWLPKLDIRTIIYSLCSSRFNSVIGVSNSVKEEFRYKQLIKDKFVLLPNTVNTERII
                     KMGNEFVEDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRVVMIGEGPLFDEVK
                     MQIQKRNLTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSVEAMLLETPVVAYN
                     VGGLKDIIDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAISAKKFSNMNRYQG
                     VLEKIYVSAALK"
BASE COUNT         1309 a          649 c          425 g         1244 t
ORIGIN      
        1 tatacctata cccagaggaa taataaaaat ggaaacaatt ttaataaaat ctaaatatgt
       61 cttatcatac tccgttttac tattttgtaa acgagataat gttgaaaata atacagttgt
      121 tgttgctgaa gttacaattg caaaaatacc attaaccgtt gtcattgtag ttttgtatat
      181 tcccaaatag tatacgctta aaatgcctcc tacgataaaa gtacctatat aagatgacaa
      241 ccatgtacct aatgattcca ctagtgacca cgcactaaag gaaaacattt cgtaaaattc
      301 tttttttgaa aaaaaaagtt ttggtttcca ctctgataac ttagttaata ttgcagctgt
      361 aactaaattt cctattatag atccaattac tagtgaccaa taacttagtc caattatcgc
      421 caacggtact gtaataacta aaggtatcaa tgcagatatt attctagcat aacacaaaat
      481 tttgtaatca aaatttcgtc taaaaattgc cgattgtata ctggaaaaag aagtaattgg
      541 aagagaaact ccagctacta taattactaa tccaagtcca gaatttccaa ctaagtgcgc
      601 tattttatta ttaaacacac aaataaataa ccataataat aaagaaatac ctaagttact
      661 agtaaaagct acatttgttg atgaaaacat gtgttcttca ttatcaaatt catgctgtac
      721 taaaaatttt tgaaatcctg aatcactaaa catatcagcg aaactaataa ccatagttac
      781 agtggctact actccaaaag catctggagt caataatcta gctaaaatca tattcgaaat
      841 tggagttata atctttgcaa ttatttcagc taaggatgac cattttaaag cttttgctgt
      901 ctttccatga atattatttc ccatttatta ccccattttg ctaaataaat atagcaacct
      961 ttcgcatatt aaacattact ttttaatcaa atttataatc aaatttcaac tctgttatga
     1021 ttgctggatt tccatatact aagctatatg gtggaacact ttttgtaact acagcatttg
     1081 cacctatgat tgaatcatgt ccgatattaa ttggtcctaa aacacatgca ccagcgaaaa
     1141 cagccacatt ttcctctagc gttggtgccc caacagtttc ttttccattt attattttgc
     1201 catttccacc caaagtcaca ttttgatata ttttacaatt attaccgatt tttgttttag
     1261 ggtggaatac acaaccaaga ccattatgga ctagttgagc attagagcta aaatctacct
     1321 gtccgtagaa ttcacatcca taaataatcc ttaaaatata gcaggatatt tttgacattt
     1381 tattttgacc attcctgtat tgtctacaag ctttatctgc ccatttctct gcttttcttc
     1441 tcattttttc tacctcaact ttcttttttt atagttcttt ctgatgcaga aatcatacac
     1501 aaaacaacta ttgcgtaaag aaaacgcgaa gatacaatat ccggtataaa cattgctccc
     1561 actactatca tcaaaaataa ccatttatag caacctgatt tctttaattc ttcatacgta
     1621 aatttaaaga agccacttaa aataattagc gtaccaataa ttcctgtctg taataaatat
     1681 tgaataaaaa aattatgtgg tactgctaca cctagtagcg tgtataaatt atttaatcct
     1741 aatccatatc ctattaagta tttatcacta aaagcattta aagaggacat atagttttct
     1801 aatctgcctg aagaatccaa aagactttgt ccagaacgac ctgccattaa cattactagt
     1861 atataaggtg tagttatgat tccacctatt agaactaaaa gatgaggaac actaattcta
     1921 tttatattaa atagataata caatgtcatt gtgacaaaca aggaaactaa ccctgttctt
     1981 gcactaacac ataaaattgc acctaataaa aatacttcta ttattataaa gaataataat
     2041 ttccaatttt ttaaatccac atattttaga aaagcatata gacagcctgt agcaagatat
     2101 aaccctgcaa aactaaaatc tcccatcaaa cctgcatatg caattctatt taaacccatt
     2161 tctgcataat ggcctattat tacaccagta gataaaatat atactcgttg caaatatacc
     2221 tgaaacgcca tagatactac tcctatgaaa tagaaattcc acccaatcat agttaagttt
     2281 ttgctataca ttttaaaacg ttttcctatt aaaaaactac agataaacaa cgaaatagtc
     2341 attaattgct ttaatgcatc tattaaaaaa ggtaagaaca tagacgtcga tattccaaaa
     2401 caaataaata aaaataaaat aatcaagtat gaacgattta atgttccttc tataataata
     2461 tataacatca aaattattat tattagatta tgccacgtca atggtaagct agaataacta
     2521 gttcctaata tagatatagt tgatgttgga atgaaaagaa ttgatgttaa caataagcta
     2581 tctgctaaac aaaaatactt ttttaagcac cacatataat acaaaaacat aaaaaaagaa
     2641 acaactgcaa ttgctattga gggcatcagt gaataaaaaa tcattcctga ttgaaacaaa
     2701 atactaatgg gtattatttt tcgtaaaact aattgatctc tatttttata attcatattt
     2761 tattttaaag ctgcagatac ataaattttt tctaatactc cttgataacg attcatatta
     2821 gaaaatttct ttgcactaat agcagcattt tctcccattt tttttctaag ttcttctgaa
     2881 tttaatagct tcattagtga attagataat tcaacttcac taccacacag aaatccattt
     2941 gtctcatcga taatatcctt caatcctcct acgttataag ctacaacagg cgtctctaac
     3001 aacattgcct ctactgatac taggccaaac ccttcccaaa gagatggcat taatagaact
     3061 ttagatttct tcatgtactt atagggatta cttttgaatc cctttaactc aataacatct
     3121 gttaagtttc ttttttgtat ttgcatctta acttcatcaa ataatggtcc ttcaccaatc
     3181 atcacgacac gtaaatgttt tgaatatttt tttactacat tttctaaaac attaattgca
     3241 cctattggat ttttaggttc tgccatgcgt cctacatata aaatatcaat atcatcttca
     3301 acaaattcat tccccatttt tataattctt tcagtattta cagtgttagg taacaacaca
     3361 aatttatctt ttattaattg tttgtaacga aattcttcct taacggaatt cgaaacacca
     3421 attactgaat taaacctaga cgaacataaa gaatatataa tagttcttat gtctagttta
     3481 ggtaaccaca agggattatt gtgcaaatgg gaaaggatga tttgcttctt atatctactt
     3541 gctcttacac tagcattaaa atcatgagca tgaattatat ctgggtttaa ttcatgtacg
     3601 gctctgtcaa tttcttttct cgaaaat
//
LOCUS       DAZUMV010000185         3605 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000185 DAZUMV010000000
VERSION     DAZUMV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.193"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..190
                     /locus_tag="ACG8FK_002708"
     CDS             <1..190
                     /locus_tag="ACG8FK_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5098252.1"
                     /translation="LVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVT
                     VYEGHFVIAFKSGIEMEI"
     gene            333..557
                     /locus_tag="ACG8FK_002709"
     CDS             333..557
                     /locus_tag="ACG8FK_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098253.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            572..1441
                     /locus_tag="ACG8FK_002710"
     CDS             572..1441
                     /locus_tag="ACG8FK_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS5098254.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1422..2156
                     /locus_tag="ACG8FK_002711"
     CDS             1422..2156
                     /locus_tag="ACG8FK_002711"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5098255.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2189..3097
                     /locus_tag="ACG8FK_002712"
     CDS             2189..3097
                     /locus_tag="ACG8FK_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS5098256.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3094..3574
                     /locus_tag="ACG8FK_002713"
                     /pseudo
     CDS             3094..3574
                     /locus_tag="ACG8FK_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1252 a          521 c          789 g         1043 t
ORIGIN      
        1 tttagtagaa gatgcctccc tcagtggcga gaacgagcga atcaatgagc tgattgaatt
       61 tatccgcaag aacaaattcc gcaccttaga atatgatgat aagcttgtaa ggaagataat
      121 ccagagcgtt acagtctatg aaggccactt cgtcatagcc ttcaaatctg gcatcgaaat
      181 ggaaatatga aaaccagaag taaatagccc atgactctac agtagagttg tgggttttct
      241 tttgcttgct gattataata atattttaat aactattgtg tttatcaacc aaatggttta
      301 taatattctt agcgtaaact tggaggtgct gtatgactac ggcagaaatg attaaagaac
      361 tgtgtgagca aatgaatata agtgtttccg aacttgctag acgtattggc cagactccac
      421 agaatttcaa taaaaaatta caacgagaaa cagtaacctt ggatgagttg aaggccattg
      481 ctgatgtgct aggtgtcaag tttgtgcagg catttatttt accagatgga gaagaaataa
      541 aaatatctaa cgaataaagg aggatgttta tatggtaggt aatgttattc aaatagtaac
      601 agagaaatta tcatctttgc cttttataga aggcatcgta ttagggggct cacgtgcaag
      661 aggcacccat acagaaaatt ctgatataga tatcggaatc tattacaatt cagactcatt
      721 tgacctgaca gcgattaatc aaattgctac agaattggat gataaaaata gaaacaacct
      781 tgttgtgcct cccggagcgt ggggtgattg gattaatggc ggcggatggt tatttataaa
      841 tgggtatcat gttgacttga ttttacgtga tataaaacgg gtagaacaaa taatcaaaga
      901 tacggagcaa ggaattgtta ctgccaatta tcagaccggg catccccatg gttatataag
      961 tgcaatgtat cgtggagaat tagcgattag taaaatacta tatgctaaga acgaaagctt
     1021 atgcgaatta aaaaagcagg cagaaattta ccctactgct ctaaagaaaa gcttgatgaa
     1081 cttttttata tttgaagctg aattctcttt aatgtttgta aaagcaaatg cgggggtaga
     1141 ggataaatat tatattgcag gccatgtttt tcgtattatt tcatgcttaa atcaagtact
     1201 atttgcatgt aataatgctt attgcattaa cgaaaagaaa gctataaaac tgcttgaaac
     1261 ttttgaatat aaacctgaaa aatatgccga aagagttaat catatttttg aagtactcgg
     1321 tctttctctt tttgaatgct acgatatgac cgagaaactt tataaagaag tgaatgaaat
     1381 tgtatcggag ataaataact ttttaaacga ggagagttca gatgaaagaa aacaaatatg
     1441 atgataatat attttttcaa aaatacagtc aaatgagtcg ctcgcagaaa ggactggctg
     1501 gtgcgggaga atgggagact ttgaaaaaga tgctacctga ttttaagggt aagcgtgtgc
     1561 ttgatttagg atgcggctat ggatggcact gtatatatgc gatggaaaac ggtgcttcct
     1621 ctgtagtagg tgttgatatt tctcataaaa tgctcgaagt agcaaaagga aaaacccatt
     1681 ttccacagat tgaatatgaa tgctgtgcca tagaagatgt ggatttccca gaggagagct
     1741 ttgatgtaat actaagttcg cttgcgtttc attatgtagc agactatgag aatttaataa
     1801 aaaagatata taggatgctg aaggctggtg gcaatttagt ttttacagtt gaacatcctg
     1861 tttttactgc tcatggaaca caagactggt attataacga aaaaggagaa atactgcatt
     1921 tcccggtgga caattattat tatgagggca aacggacagc tatgtttttg gaagaaaagg
     1981 ttacaaaata tcatagaaca ctgaccacat atctaaatac actgctttca aatagtttta
     2041 taataaatca gattgtggag ccacagccgc cagagaacat gatggatatt ccggggatgg
     2101 cggatgaaat gcgacgccca atgatgctga ttgtatcggc aaaaaagaag atgtaataat
     2161 atagaaaaaa taaacgagga gtatgtaaat gagatcagaa aaagaaatga tggatttagt
     2221 actttcttta gcagaacagg atgaacgtat tcgaattgtg acccttgagg ggtcacgcgc
     2281 aaatattaat atacctaaag atgaatttca ggattatgat attacatatt ttgtaagtga
     2341 tatagaaccg tttatatcta atgatgactg gcttaatcaa tttgggaata taataatgat
     2401 gcaaaagccg gaggatatgg aattattccc acctgaagaa aagggatttt cctatcttat
     2461 gctatttgat gattacaata aaattgatct taccttattg cccttggaag agttagataa
     2521 ttacctaaag ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag
     2581 ggacatagtt ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga
     2641 tgattgctgc aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa
     2701 ggaaatttta tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat
     2761 gatatcatgg aaggtcggca tcgaaacagg ctttaaatta agtgtaggca agaactataa
     2821 gtttattgaa aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat
     2881 ggattcctat gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt
     2941 atccggtgag gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac
     3001 aaaatatacc agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac
     3061 atatgccgct gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac
     3121 ctgaaagata tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg
     3181 tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc
     3241 taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat
     3301 actgataaga taatatatct tttatataga agatatcgcc gtatgtaagg atttcagggg
     3361 gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt
     3421 gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat tctatcataa
     3481 ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     3541 tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt
     3601 cgtct
//
LOCUS       DAZUMV010000186         3513 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000186 DAZUMV010000000
VERSION     DAZUMV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.194"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..1380
                     /locus_tag="ACG8FK_002714"
     CDS             49..1380
                     /locus_tag="ACG8FK_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFS5098257.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1346..1696
                     /locus_tag="ACG8FK_002715"
     CDS             1346..1696
                     /locus_tag="ACG8FK_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098258.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1788..2207)
                     /locus_tag="ACG8FK_002716"
     CDS             complement(1788..2207)
                     /locus_tag="ACG8FK_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS5098259.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2208..2552)
                     /locus_tag="ACG8FK_002717"
     CDS             complement(2208..2552)
                     /locus_tag="ACG8FK_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS5098260.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2902..3498
                     /locus_tag="ACG8FK_002718"
     CDS             2902..3498
                     /locus_tag="ACG8FK_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5098261.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          921 a          641 c          639 g         1312 t
ORIGIN      
        1 tctttcattt caagttatag ttaaagaaaa tgaaaaagaa ggtgtgtaat ggaaaacgaa
       61 actgacgtta tttatattca tcctcaaaaa cgaatcgttt cacagaaaag aaagtatttt
      121 tatcttggtt ttacaggtgt tttttttctt tttattggct tgctttctaa tactcctact
      181 gataattgga gtggattgct aactattctt acctcgccaa gcaatctttt gacagattat
      241 tttgctttag gtgttttcgg aagcgcattc ataaatgtag ggattttgac cttactttct
      301 gtgcttttag cttatcggca caaggtgatt ttaaacggtc ccttatttgc ttcgattcta
      361 acagttaccg gtttctcttt ttttggcaaa aacctttata attctatctc aatcatttta
      421 ggtgtgtatc tatacgctgt atttgtcaat aaaccttttt ctcagtacat catgatcgga
      481 ctgtttggta gcgtattatc accggtcgtt agttatatca cttttggtat gcgttttcct
      541 ttactgatag gtattttatt ggggaattta gctggcatag caattggtat acttcttccg
      601 cccttagctg ctcaaacttt agttttccat cgtggattca cactttataa tattggattc
      661 acctctgggt tgattgcgat gacttttact gctgttttga ggttattcag ctattcaatc
      721 gtcgaaaata cattggtttt caatgaatat cactttcctc tcatttggat tatttttggc
      781 tttttttcgc tgacagtcgg aattggtttc tattataatt cctttcggct ttcggggatt
      841 cgagaaattt tcgattcatc tggaaaattg acaactgatt tcattgctaa ttctggtatt
      901 ggtgctactt tgatcaacat gggcttagtt ggtctgatgc tgtcttctta tgttttgctg
      961 gttggcggtc agttaaatgg acctgtcatc ggtgctattt tatctgcggt gggtttcagt
     1021 gcttttggtt gtcatttaaa aaacagtttt ccgatactag tcggaatatt tattgcttct
     1081 ttgtttggaa cttttcatga gatcaccagt actggcatgc ttgttgcagc tgtgtttggg
     1141 acaggtcttg cgcccataag cggtttttac ggcagttttt acggtgtaat agctggtgtg
     1201 ctacatattg ctcttgtcca taacgtcagc actttgcacg gaggactaaa tctatataac
     1261 agtggttttt caactggttt cgttgcaggt atccttgtac cgatactaga taactttacc
     1321 gctgtcagaa aggaaaaaaa gacacttgga aaaagaatta tcaaaaagaa tcatcgatga
     1381 gcttcttact tatttatatt cccatgaaat aactgaaata cgtattggag tagaatttgc
     1441 tgaggaaggg cttttcatag agatacaggg tacggcagaa actcagccag aagatttaga
     1501 tgtacttgct gatttgctga acaatcctcg ggaccctagt atggaaatct actatcaaga
     1561 actattaggt aatccccgac atgatcggga agattatcat ctgttaggta gtttgattga
     1621 tgacgtggaa atcatgtatg cccagccgat tatcaatatt aagatctttc gacgtgcttc
     1681 tcattcctat tattaaccct attttctatt tataaaagac tgaatcaaaa aagcagctcc
     1741 tgaaagttcg attaaaatct actttcagga gctgttcaag cttgacatta ttgattttcc
     1801 aatccttcat acacttcatc tgttacaggt tctaaaaact ctgaattacc agcagtgatc
     1861 gccaaatgtt caaaccaact gtcttttgtc gcaccatgcc aatgtttaat ccctttgtgc
     1921 gttacgacga catctccagc cattaatcgt cttgctggct ttccttcttc ctgataccag
     1981 ccttctcctc cggtcacaag taagatttga tagccgtctg tatggacatg ccagtgattg
     2041 cgacaacctg gttcaaaagt tacattacct acaccaacag aaacttcggg atcattcact
     2101 aacatattca gatagctttg cccggtaaat acttgagcgt aagcttcgtt tttttcacct
     2161 gctggaaaaa tcacgccatt gtgtatttct tcgtgttttg tcatcattta cgcctctttc
     2221 ttatcttcgc tattttcttc taaatagatt tcttttgcta aactaaaagc tgaccaagct
     2281 tttggccatt ctacataaaa agctaaatgg gtaatgattt ctgctatttc ttcactagta
     2341 atgccatttt gcttgccaat cttcagatga gctggcaatt gttcaaaatt tcctcctgaa
     2401 attaatgcag atatggtgat gatactacgc tgatgagcag ataattcttc tctggaccat
     2461 acttgtccaa ataaaacatc atcatttaat tctgcaaatt ttggtgcaaa atctcctaat
     2521 tgatctcgtc ctgcggtttg tttttctgcc atttctctta ttctcccttc acgactgtta
     2581 ggtgattcaa ctatggttct aaggcaactt tattttctat tgtttcttcg catttcattt
     2641 taaatgtagt ctttcagcct gtccaatgct tagtttattc agtcgatatg ctttttaagc
     2701 atagttgcta acgctttttt tattttaaaa aaaacaagtt attaaataag tctttagttg
     2761 gatgtttttt tgagatcaat cttttatact tatgatgaac ttacaaaatt gtttgttttc
     2821 tgtcatatca aaaaatggcg cagttagttg cttttctagt aaagtatagt taacaaagaa
     2881 cctctaagga gttgatgaat aatgaatatt gctgcctctc gaaaactgat aaatttcata
     2941 tccattatcg gattaggcct atcaatcgcg ctgacgattt attttattaa tttaggtgtt
     3001 ttcaaagatt tgaatgcgtt aagaggatta gtaggtgatt ccatcatttt aggaccgatt
     3061 atttttgtct tcatccaaat cctgcaagta gttattccaa tcattcctgg agggatcagt
     3121 actgcagctg gtgtcctgat atttggccct tatgctggat ttatctataa ctatattgga
     3181 atctgcattg gttcgattat catttttctg ctagggcgtc gttatggaaa gccattcata
     3241 ctttccatga ttagcgataa gacatataac aaatatattg gctggctgga taatcaaaac
     3301 cgttttgaga agctgtttgc tctagctatt tttcttccag ttgctcctga cgatgcgtta
     3361 tgtttgatgg ctggattgac aaacatgtca gtgaaacgat atacactaat cattttgatt
     3421 gctaaaccac tgtcgatttt cttatatagt atggccttga tttatggagg tcaatatcta
     3481 agtggtcttc ttggataata tttattttgt aaa
//
LOCUS       DAZUMV010000187         3478 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000187 DAZUMV010000000
VERSION     DAZUMV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.195"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG8FK_002719"
     CDS             complement(<1..2232)
                     /locus_tag="ACG8FK_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5098262.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG8FK_002720"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG8FK_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          977 a          769 c          549 g         1183 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataata
//
LOCUS       DAZUMV010000188         3423 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000188 DAZUMV010000000
VERSION     DAZUMV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.196"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..200)
                     /locus_tag="ACG8FK_002721"
     CDS             complement(<1..200)
                     /locus_tag="ACG8FK_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098263.1"
                     /translation="MKNFDTYYVIVFSISLLLILLTLMQYKEYPEYFILTFVYLVSSY
                     TLLYKFRSSSRVSKVCFLISLL"
     gene            complement(214..444)
                     /locus_tag="ACG8FK_002722"
     CDS             complement(214..444)
                     /locus_tag="ACG8FK_002722"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS5098264.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(538..876)
                     /locus_tag="ACG8FK_002723"
     CDS             complement(538..876)
                     /locus_tag="ACG8FK_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098265.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1804..1920)
                     /locus_tag="ACG8FK_002724"
     CDS             complement(1804..1920)
                     /locus_tag="ACG8FK_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5098266.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(2396..3274)
                     /locus_tag="ACG8FK_002725"
     CDS             complement(2396..3274)
                     /locus_tag="ACG8FK_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS5098267.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         1105 a          618 c          497 g         1203 t
ORIGIN      
        1 atcaataagc taattaagaa acaaacttta gaaactctag aagaacttct gaatttatat
       61 agaagtgtgt agctacttac taaatataca aaagttaaaa taaaatactc tggatactct
      121 ttatactgca ttaaagttag taatattaac agcaaagata tcgaaaaaac aataacataa
      181 tatgtgtcaa aatttttcat aataagaact cccttactgt tgcatactaa cataatccaa
      241 aaactcttct acttcagcta aagacattcc actttcttta gtccaaaaaa cagttttttc
      301 tatcgatcct tctctgtaaa ctgtaaatgt cggagtcgag ttaattgagt agcgactctt
      361 gaattctttc cactcatttt catcttctct aacttttgct acattcacgt aataaatatt
      421 ttcttttccc ttttctacca gcatatcgaa tagcttcctg cttttgatga tcgatttctt
      481 gtgctacatc tagtaaattt tcatcaattt ctatatcata aagtatttca ctagtgtcta
      541 catccacaat atatactttt tatcagtagc aatttttgag taatccgtta aaatgatctc
      601 agtctcgtca ctttcatcag tataaatata cgttaacaat gaactcacct tttcaatcgg
      661 tgttagctct aagcctactg tattttttac tattgagtca ttatttacca ccataacttc
      721 gtactgtata taccatccta tttggtttcc atcaatatca gtaattggct cactagctaa
      781 tggagtctcc ggaacaatat tttcctgata tttcttaaac tcttctgtag cacgaaaaag
      841 gttatcatgt tcctcacttg aaactggttg agtcatagaa aatgttactt ggctaaacag
      901 agggaaaata aacactacaa tcaacaaagt ataaaaaaaa gcccttcttt ttcatttttt
      961 atcttccaat cacaaattga aaaacttatt cctgcaactt tgagtctagt atcacaatcg
     1021 actgactaca actattcatt tacattctac tttaagttcc tccttaacca ttaattttat
     1081 taaagatact tgtaatagtg attctcacaa aatttcatga aaaatacttt attactgtat
     1141 ctaaaattcc aatacacaac ttatcgacta ttatctgaaa accaaaactt attattcctt
     1201 gatgttattc tagcaatagt aactgctttt ttattttttt gcatattttt tcggcgttac
     1261 tttttaaagt taactttttc gacaaataga aagagatacg aatcactgat ttttttgaag
     1321 aaaagaaatg gagtgagctg aacgttagag atattgggag ctttcgtaac tgcttattaa
     1381 acgataatgg ttatttgcaa ccgtattgct cgcttctaca tgaaatattt catgaaatat
     1441 ttcgcaaaac ctttgattat gtagttacgt ttccattctt gactaaaaat gtttcaaaaa
     1501 cgaacaaagg tgttaaaagg taaagtgatc aatactactg gtcaaaataa gaggttgaaa
     1561 gggaattgaa aaataactac agataatact tttataaacg tacgtgcttt gtggttctgt
     1621 agttaactat ataactggaa ttagggaatg gtatcaattt gaaaagaaag attctaccaa
     1681 tttatcatat gttaaaaatg gaaaatcaac agcagttctt tcggaaacct tacacaaaaa
     1741 ccgattattg aaaatagccg aaaaacgttt ttcggctatt tttttaatta agtttccttc
     1801 ttcttacctt ttcccactat tccgtttcac atctttgatg atcttcagcg cacgttttcg
     1861 tgttttgata tttgggcttt tcaagcggcg gtatgcactt gagatatctg ttttttccat
     1921 atggacgacc cactttcttc tttgattgta gtcatgctaa aaaacacaaa tcgaaacaat
     1981 tacgatttgt gttttaaatg gtatcaaaag ttaaaatgaa agtcaacttg ttaaaacagt
     2041 cgatcagact agctgattga ctcctatctc tggaaataaa ttttcattat tacgttttag
     2101 ctacctaaaa aaggccctct tgtccgttaa ctttaatatc tattgttata gctagtttac
     2161 ctattgaacg tactgtgtgt tcgatagaac actaaaaaag aggctgggac agaagcgttt
     2221 aactccaaga aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttcag
     2281 cttatttccg aaggagttgc ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca
     2341 aaagtgttct ttacttttgt ctcacgctct tttaaactat ttcgaagtac taaacttatg
     2401 aagtaagaat acctaagcca gagccaatgt gctcgcctgc aagcaattgc tcttttgctt
     2461 gtcgctgtaa actcgtactt tctggataat catgtatttg ggcatctact cgtccaaaga
     2521 tccaagggac tccactatcg tcctgttcat caactgataa taatcgataa tcggctgcaa
     2581 aaagacagat ttttgactct tttttcaacc atttagtaag attgggtgat agtaaccaag
     2641 tatctgtgta catcacttgg taatggtaat caggaaaata aagttcaaaa aaacgaatgg
     2701 cactttcgta actatctctt atcttattct gagataaatc tgcatcggaa ggaatatgaa
     2761 gagaaaggca ctgctttcct tctaatctta gatccgtgta ccctttcgga aaatatgtca
     2821 tttcgtactc caattcacca atccgaaaga tcaagccact agtgtaatgc caaatccaaa
     2881 aaccacggtc aaagcgatag cttcctgtta cttggaaatg ttcatgaata aatctagtaa
     2941 aagcattcat tgttgccaaa tagatagttc ttggaatctt caatggttga taaatttttt
     3001 tccaactttg agaagctgca tatagataaa tagctaatgc tgcaaaaggt tcctcttcat
     3061 agactttgtc tattttcttt gcagtcgcct ttgctttatt aggatccatt agttcatgac
     3121 tatattgctc aaacacctca tcaaggatgt tttttgcttg tacaatcatt tgctctataa
     3181 cgactttggg aagagttaaa gtttctgcca gaatttggat ttcattgctt attttcttca
     3241 tcaaatttcc ccttttcata tctttgaacc tcattgttta aatgactgat tatccggaca
     3301 ccatatggta taaacatact tttattgtac actaactata aaacttaaac tttctatgaa
     3361 gaaagaatat ctgtaagact ttcaaggaaa gcattgacca tctcaggaaa gtttattcag
     3421 tta
//
LOCUS       DAZUMV010000189         3404 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000189 DAZUMV010000000
VERSION     DAZUMV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.197"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(141..1724)
                     /locus_tag="ACG8FK_002726"
     CDS             complement(141..1724)
                     /locus_tag="ACG8FK_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098268.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            1846..2430
                     /locus_tag="ACG8FK_002727"
     CDS             1846..2430
                     /locus_tag="ACG8FK_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098269.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2441..2686
                     /locus_tag="ACG8FK_002728"
     CDS             2441..2686
                     /locus_tag="ACG8FK_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098270.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2692..>3404
                     /locus_tag="ACG8FK_002729"
     CDS             2692..>3404
                     /locus_tag="ACG8FK_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098271.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQS"
BASE COUNT         1189 a          465 c          534 g         1216 t
ORIGIN      
        1 agattctaaa gaaagaagag ggaaatatat aaatcaagcg acagtatatt tattattaaa
       61 tgctattgtg agaatataac taattagttt aatttgaaag taaatatacg ctataaaagt
      121 gcatcttgtg ttataaaaaa ttattttatt ttgtgaacat aatttaacaa ttctttctcg
      181 gaattgattt cttttcttaa atagtcttca gacttattaa taatattatc tattatgtct
      241 agatacatat cacttttcga ttcagctaat tcatacagtt tttttaattc tttatccttt
      301 aattgaaaac cctgatccat agtcactgca tttggatata gaagcccact ataatgttta
      361 cgtaaattca cttggaattt ctgattataa aagtctaaca gattagaaat aaaaggcgga
      421 aaattactgt ttttgtaaaa ttgattattt ccatttaggt tgtatagtaa tttgttaaat
      481 tgctcctgat ttaatttatt gtaaatccat ggtaatactt ccatatcctt atctgctaac
      541 ctgaatgaac tagcatcggt aagatgttcg atcataaaat ctatgaacat ctctttttca
      601 ccaaaatata aatattgatt atatattgtt tctaatgttg aagatggtag cgaagaaact
      661 ctcatttttt gctggagtat aggatcaact agactattta agtgattttg taattcttca
      721 ttgttgtttc ctagaactgc aagggaacgt aaacggtagt aatattctat ttctgttagt
      781 acttgttgtt tcggtttaaa atcatttatt gatatttgta aattttgaat tgtctcgtct
      841 gaaaaatcat gttgtaattc tggatgggta gctataaaag taagtaaaaa gaaccctttt
      901 gtatacgtga tctgttgatt ttcaccaagt gatttattaa tttcctctct tgattcagct
      961 gtgaacctat actttgcaat ttcttttttt agaatattta aatagaattc tttattatca
     1021 aaaattaggg cattaagtag tctgaaagta ctttccctat ttctatctgc ttcctcattt
     1081 ccataagata attgaaaaac aaacttaaag gctgtcttac ataggttttc tttggctttt
     1141 tcgcttagtt tatttagata gctatttttt agtgcagagt gaaattcttc aggtgtaaca
     1201 gacatagcat gtatgcgact tgcatattgt ataatttcga tcgttacttt ttttcctaat
     1261 attggatttt ttaggaatat aatttcatag gacttccgta taacatcctg acaagtttct
     1321 tttgttatat ctcttaattc ccaagtatta ccgttgtaag tgactacagg atgagctgaa
     1381 taatttcttt gatccttaat atattttaaa tcctcatatt catagctatc aattagctca
     1441 gttgaactat aagctttttc tagtagttcc atttcccatt ttggagagtt tggatcactt
     1501 tttcttagtc tagctatatc ttctaaaatt ttcttagaag tattatcctc gtaaatattt
     1561 gctaaatcag ataatttatc aattaaatct aaaataagcg cactgtaatt gacagctatt
     1621 gcagaacgat aatttccctg ataaaaacta cttctaactt cttctaataa tggaataatt
     1681 ctaggatttt taactttttt tatttcatca tctaatataa acatataaat ctcctttttt
     1741 tatttgtatg acaagtttac cataaaaaaa aattaaaaag cgagacatat ttatgttgtt
     1801 gtgttatcat aataacataa atatgtagag tgagatgatg aaaaaatgga tttgaaacaa
     1861 tcgttttaca tctggttata tcctgataat cctatgtcaa taatggctga tatttttgta
     1921 gttagcatct tattgtattt attttatcta attttctttc acaatttaat cgggtacatg
     1981 attcttgatt ggaaaggaat gttcgatata gaaagaaaac catttgctaa caatcaaacc
     2041 tataccgtct tcgaaagtgg agattgggaa gaaaatgaag ttgatgtgat ccatgaaatt
     2101 ggattagctt tatcaggaat acaagatgct gatgttttgg tatcaatgac aacggatact
     2161 ggatacaacg cttatgaggt attttatctt atgtatttta tatggcgtga tggtcaagaa
     2221 attatcaatg gtcgtgctga aaaaacaatc aattctaata ctgaaatttc tttttgtatg
     2281 ggtgaagggg aaatgattgt atataaagat gggtcagcaa acattaaaac agcctttggt
     2341 gatattgaaa caagtattaa ggaacttagg gaatatgtgc agaacccata taattttcaa
     2401 tatttagcag gaagaataaa tttagtttag gagtaaaaat atgaaaaaaa ttataaatca
     2461 accaacaaat cacttgataa ttgaaggtgt tattttcgta acaatgtatt ttactgtagg
     2521 aaatggcatc tcaaatacaa tcattcaaaa aaatccacaa tacctattat ttagtcttta
     2581 tgcttggatt gtacaaatgg tttgggtttc catattgatg ctgttgtttt attggatagt
     2641 acgggttatt cttttattgt taaacaaaag aaaaaaggat aggtgaataa aatgaaatca
     2701 actaaattga aggttgtatt gttatcgaca gcagcattaa taacccttac agcatgtggt
     2761 acaactaata gtgctaataa taacaaagac acaaagtcta gtagtacgat ggttactaaa
     2821 aacagcacta catcggcatc aacaacaatg tcttcaatga ctgaaaataa tgctgaaaag
     2881 tatcaaaaat acgaagacat taataaaggt caaaagccgg atgaagttta taacaacttt
     2941 attaatacag taaaaaatga tgaaaattat gggaatgatc cattgcaaaa taaaaatctg
     3001 actatcgttc tttacaaacc tgattgtcca gattgtcaaa aagcagaatc aaaaatcatg
     3061 caagaaataa aagaaggatt tagttatgct ccaacattaa tgcatgatat taccataaat
     3121 aaaccagctt acgagtatta cgcagttgct gtagatatta ctgatggtgt acctgagtgg
     3181 gtacaaaaca ttcctggtct ggctgatgag aacaatcaat atcatacgcc aacagttgca
     3241 gtagtaacac caaagaaagt taacggcaca ctatactggg atgtaatggg aatgacaaca
     3301 aatacaactg atgaaatgat tgaaagtaca actaaatatt atatggattt gggagcatat
     3361 aaattatatc aacaatcaga gcctgttatg gacgatcaat ccaa
//
LOCUS       DAZUMV010000190         3339 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000190 DAZUMV010000000
VERSION     DAZUMV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.198"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            652..738
                     /locus_tag="ACG8FK_002730"
                     /pseudo
     CDS             652..738
                     /locus_tag="ACG8FK_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            1292..1516
                     /locus_tag="ACG8FK_002731"
                     /pseudo
     CDS             1292..1516
                     /locus_tag="ACG8FK_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1776..2030
                     /locus_tag="ACG8FK_002732"
     CDS             1776..2030
                     /locus_tag="ACG8FK_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5098272.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            2031..2348
                     /locus_tag="ACG8FK_002733"
     CDS             2031..2348
                     /locus_tag="ACG8FK_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5098273.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            2907..>3339
                     /locus_tag="ACG8FK_002734"
     CDS             2907..>3339
                     /locus_tag="ACG8FK_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098274.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT         1204 a          459 c          562 g         1114 t
ORIGIN      
        1 aaaaaaatta ctttaaagta gttacatact acctgttaaa ttagatacta ccattaatac
       61 ttaaattaga tactacctat atttatatta agtactaccg ttctaggaat aagataataa
      121 aaaaaaacta ccaaattgca attaaatact acttaaaatg taaaaaataa gaactaccca
      181 aattaggtag ttcttaataa gagaatatca gataaatttg tacaagtcat aaaaaaatta
      241 atcttaaatt tttaaatata tatacctgtt aattataaac tactttattt aggtagtaca
      301 taattaacag ataaaactac atgcttgtag tttgtctagc taatgagttg catatgattt
      361 atataaaggt gtgcagcata acttagttga agttaagaga atagaataaa tattttttgt
      421 tttgctaata attaaacact acctaaatat ggtagtgttt aattatataa tattcatttt
      481 gttacaaata atcgtattat aaaagcataa caattgggat aactattttt tagattattt
      541 aagtactact taaattaggt agtacttaaa ttttggttta aggtagtagg taattaagaa
      601 ataatatata tcttctttta taatagaagg ggagaggcct agttaaaaac agcgacaatt
      661 aataacaatg tatttaatgc ttcacaaaat ccatttggtg gacaatgcat tgttattagc
      721 gatgatctac cgttctaagt agttttttct attggttgca ttcgtgacta attatggtat
      781 aattatgagt aacaaagagg tatttcatta tatcatcaaa ccccatcagc gtgtaggctg
      841 atggggtttt ttttacgttt ctatattata gaattaactg gtaggcttac tgtctttttt
      901 ggcaattttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      961 gattgcaaaa acaaaatcaa aaaggtattt cattatatct cctcctcccc ttgccagatt
     1021 aggccggggt agcagacagc aaaactatta taacataact gaaataccta aatgacatat
     1081 acagatatga taatttatgt attgattttt ctaacaaaag cacatatttt aatatatact
     1141 acctatttta ggtagtatat atttttagat acaatatcga tcagttttga ggaaatgaaa
     1201 ttctagatat tttttgctac atagagttac tttcaatatt ttttaacgta attaggcaga
     1261 agtcactcat cctctatagg tttgtgatga aattgctatt gatccattta agtaagagca
     1321 aaaaactgga ggtttatcag gtaattggtc taatggattt aagtatgcgg tcaccgtgta
     1381 tagagttgct tatgagattc aagaaaataa agttattcca gtattgctat atggaacaca
     1441 taacaatttt tatgaattag ccgagaacac tcgcaacttt attcgtgtag atgaatcggc
     1501 ttcggttgag ttataacttt agctatatca atcaaccgac cgacatatgt taaatctata
     1561 tataataaaa atacctactt aaatggatgt taacggaagt gtatggtgag tagttcactc
     1621 gtattttgat atcgtttgta tatgttttca gcgttctaag tggcttattt tgtccaataa
     1681 taatatggag tggaatccct taagaaacac cgttggcaat tgtcgttata acatgttata
     1741 atttatttat caaaaactaa aagaagtgtg ataacatggg aaaagaacta aaggttagaa
     1801 agatcggcaa tagcgtggga gtgatccttc caagctctct tggattaaaa agcggagaca
     1861 ctatccaagc caaacaagaa ggcaacttat tcatattaga tactactcaa atagcaaaag
     1921 aacacgatcg caaattgatt gaagaaagtt tccaagactt tgagaaaggt ttaacagttt
     1981 cagaaattga aatggtaaaa gctttcggaa aatacggctg gagtgaatag atgaaagagt
     2041 acacgctagt ttattctaaa agtttccaga ccagccttga aacgataata gaagaatggg
     2101 aaaatgagct tgctctttct ccagccagca taaaaagatt tgtaggtgcg atccagaagg
     2161 ctttagaact aaccaaaaat tttccaacta tgcatgagga agtttccgaa atctatggaa
     2221 tggatatccc aacatatcgg atcgtgatcg gcaaaagcta tgccttattt tatcggatcg
     2281 accatgaaaa aaaagagata cttgtaggac gtattttcca atctaaacaa atgaaactag
     2341 gcttttaaca ataattaggc aaaagtcgct catcctctac aggtgggtga ttaatgccgc
     2401 tcgatatggt tgttaaccgt cggttactcg aagattgata tataagactt agtacctttt
     2461 tattttgctc catgttatgg ccgaaggtct aaagatacaa tcattagtct agagaaaaat
     2521 taagaaccgt aggaactacg gggatcgctt ggtgaataag agacactgct attagtgaaa
     2581 aaataagata tcaagtacgc tctattccca aaaatttcgt gactttactc atgagaggtt
     2641 caagtagtat atttttaatc aaaattgacg taacttttaa taaatactac ctaaataagg
     2701 tagtatttat ttttgtgttt taaaagagga agtccaatat atctatggga aacttcttaa
     2761 atattatgtt ggtacatgaa aatatacatc cctgctagac tatttacaac attttacttt
     2821 gtacgtattc ttgttttaaa aggaatggga taaaatatag gtacaataac tatttgatgc
     2881 taaatataat taatggagga gagatagtga acatacttaa tgaaaaaaga tatgtatggt
     2941 ttgctggtaa aaaatatatt actactttca aaagattatc cgagttaatg cagcagactg
     3001 ttaaaagtgc tatgcctgaa ggatacaagt ttaattttgt tcaatcttca gtatctactg
     3061 aaagtatcta tttttcaatt ctttctgaga gtaggctttt tcattttaga ataagttctc
     3121 atgtaaagaa aactgatgtt gatatatata cgttccgtac aaataattat aaaaatttaa
     3181 atgatttgag tagaagtatc agtgaatatc tcaaggggaa tagctatctc aagattcgat
     3241 tttcacatat attaatttta ctaccatatt tgaatgagaa tttcacccta attcaagtag
     3301 ataagtcttt cattgcctca ggtaatcaag gaaatatga
//
LOCUS       DAZUMV010000191         3295 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000191 DAZUMV010000000
VERSION     DAZUMV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.199"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..295)
                     /locus_tag="ACG8FK_002735"
     CDS             complement(38..295)
                     /locus_tag="ACG8FK_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS5098275.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            453..716
                     /locus_tag="ACG8FK_002736"
     CDS             453..716
                     /locus_tag="ACG8FK_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098276.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            740..928
                     /locus_tag="ACG8FK_002737"
     CDS             740..928
                     /locus_tag="ACG8FK_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098277.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(1011..1196)
                     /locus_tag="ACG8FK_002738"
     CDS             complement(1011..1196)
                     /locus_tag="ACG8FK_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098278.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1411..1782
                     /locus_tag="ACG8FK_002739"
     CDS             1411..1782
                     /locus_tag="ACG8FK_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5098279.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1932..2004)
                     /locus_tag="ACG8FK_002740"
     tRNA            complement(1932..2004)
                     /locus_tag="ACG8FK_002740"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1969..1971),aa:Gln,seq:ctg)
     gene            complement(2050..2478)
                     /locus_tag="ACG8FK_002741"
     CDS             complement(2050..2478)
                     /locus_tag="ACG8FK_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098280.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2538..>3295)
                     /locus_tag="ACG8FK_002742"
     CDS             complement(2538..>3295)
                     /locus_tag="ACG8FK_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS5098281.1"
                     /translation="KKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSSTQ
                     KTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDYV
                     PVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLDD
                     IKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIYV
                     NDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1099 a          526 c          619 g         1051 t
ORIGIN      
        1 catctttaat cgttttcttt ttaaacatgc gagggattca attctgattc ttatataaaa
       61 aatagatcca aataataaga aaaattactg aaagcgtcaa ttcgatgatg tgtgttatta
      121 atacagacgt tgtttcccaa tgcctaaaag aaaaaatttg ataagtaaaa tatacagcag
      181 gtaaagaggc cagtaaaact gaaaacttct tttttgtcca ttttatgtaa tagaaaagaa
      241 tgcttatcat aataaaaaaa ggaatataaa aaacaaattg accgatcgcg atcatactat
      301 tgcctccgat aaataaagtc catgatacaa aataacactt tgtgcgaaat ggcgcaagca
      361 gttttgtgga tttatccggt aaaatctgta tcgattttga aattagagga gaaacttgct
      421 ataatcaaag tgaaagaagg agtgggattt tcatggatca tgtagtgtta gaaatcgatt
      481 tgcatttgat caataatatt cgggtgattt actatgttgt aagtaacagt gtcgaacaac
      541 gtgtactgac gaataaaata aatggtattt tagcaaagaa agacgttcac cgattcaata
      601 atggtgaagg aagttattat agtatcccag tagaaaaaat tatttatacg acagtcaaag
      661 tcagagaaga tctagatacc aaaaaagcat atgaacctat ttttacaact tattgattga
      721 cagcagatgg aggaaaaaaa tgaatccata tgaacaactt attcaagatt tgatcgatgg
      781 aaaaatcgaa aagattgatg taaaaagaga agaattaatg gcttttcgag aagcgtggct
      841 gaagttggag aatcgtaaat atgtggtggg aaaagcctca ctgaacggca atgtgactta
      901 tcattacgat ccgacacgtt tattttagta tgaagtattc agaaaagaac caaaggagga
      961 cgatgcagca gcattgtcct cctttgattt tacaaaagtg aaagttttac ttatttgaat
     1021 agatttttaa atttgttttt gacactgtcg atatttacat cttttaaaag attttgggct
     1081 ttctctttat aaggtcctaa ttgttcttta tgttcttcga taaatcgttt tgcgccatcg
     1141 aaattgccgt tcttaatcat ttccttgact ttttgaaata attcttcttg attcattttg
     1201 aacacatcct ttgctatcaa catgattttt tacatggaca aaacgagaaa aactgattta
     1261 tccacacgtc aataatatca aataacgatc atttttacta aaaaaacact cgattctcgc
     1321 ttctaaagta ggagaaaaat ctccaacttg ggacggatgg aatttcttta actgacccat
     1381 atactgaata tataacaagg aggtagaaag atgaaagtga ttaaatattt gattggactg
     1441 gcggcgatta gtggtatttt gctatttgga ctgaaatatt atactgaagg aagttatgga
     1501 gaaatccctg gtatacttga tcagctaaat ccattagttg aaaaaggaga ggtctatgta
     1561 aagacacaaa aaccagagga agtaaatgaa tatggtacag caaggtatgt gcagaaagct
     1621 gctgatgcca atgggaaaga acgaacggtt gaatttaatg gtctttctgt gttaaaagaa
     1681 aatcattatt tgaaaattag caataaagga gcacacatag aaacgtatga ggaagttcct
     1741 agagaaaaag ttccggagaa agcattagca attattgggt aaatcagttt ttgtattaag
     1801 aatacgcccg attttattat tcataattta attatgtaaa ctaaattcta gtagtaaaaa
     1861 taatacaata atccccctgc aaaaagattg ttcttttaca gggggttcat tttttcagca
     1921 agcacatcaa attgggatgg cagggttcga acctacaatt gcttgattca gagtcaagtg
     1981 ccttaccagt ttggctacat cccatttatt caacaaaata atcatagcac tttcctaaaa
     2041 aaacgcaagt tatttttctt gatcggatag ttgttgcttt actcgtaaaa ggcggcgtac
     2101 tagtttcttt tctaatccag caggaagggc aatcagttct gctactgaca agacggtgtc
     2161 caataactga tattctttga caggttcatt cataatatga tagatgtttg ctaaacgttc
     2221 aatatattca aagcgagtat actctgttcg agcaattaga aatttcaata aatcggtggc
     2281 tttctcgtac tcttgatctg cgtaataaac aagagattga cgatacaatt tgatttccag
     2341 ttcattgagg gtagcaggtc tgcgacgctc gttccactca ttaatagaga ctttaccttg
     2401 aacatttccc ttgcagtcac ggacaaaaat aatcggaatt tcatttggat ccattttttt
     2461 atgtgtgaat ttttccatta tctacgctcc cttcaaaggt ttggtatctt gagtataagg
     2521 gatttttgtt tgaattacta gcttttctcc aaagttagca tctgtccacc cattgcagtg
     2581 aaaatcgccg aaaaatcgtt gatcggtact gctcgatttt ttacaccata gggatcgttg
     2641 acataaatat tttctttgtc atatccagtg actacacaag catgaacaag aggagtgact
     2701 cttacttctc cattatttgt ggtccaagtc ataaaatcag tttcatctgg gacttcaaaa
     2761 ttgatagtca cagaagtcca gacaggattt cctttagata gtgctttttt gatatcatcg
     2821 agttgagcat cggtttttgg aaaagtgtga taatccgttt ggacgatttg ctgagcaagg
     2881 gaggcaactg ggggaacata gacgaccata gcccagtctc cgccactgat atcaccaaca
     2941 aatccttcat taggatcgcc atgagtccca tctgcattga aaacagggac gtaatccaat
     3001 tgctcagcta actggttttt agttgtttca taaccataaa aagataaaag catagaaagc
     3061 gaagtgactt cacagccatt ttctaaagaa ggagaatcaa actggctctc taatggaaca
     3121 tctagtaaaa cttctgcttt ctcttttgtc tttttcgttt tttgtgttga acttttttgt
     3181 gcagtgttca ttgtatgttc tggagaatgg atatgtaggt aataacttgt accgcttaat
     3241 aaaagagcag ctgtgccccc tagaaacaat agttttttga acgttttttt cttca
//
LOCUS       DAZUMV010000192         3161 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000192 DAZUMV010000000
VERSION     DAZUMV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.200"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..805
                     /gene="fabI"
                     /locus_tag="ACG8FK_002743"
     CDS             50..805
                     /gene="fabI"
                     /locus_tag="ACG8FK_002743"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS5098282.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(915..1967)
                     /locus_tag="ACG8FK_002744"
     CDS             complement(915..1967)
                     /locus_tag="ACG8FK_002744"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS5098283.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2093..3013
                     /locus_tag="ACG8FK_002745"
     CDS             2093..3013
                     /locus_tag="ACG8FK_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS5098284.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          930 a          639 c          604 g          988 t
ORIGIN      
        1 tattttttta cgactatcaa atttttatca gaaacaaagg agagtcatta tgtcatttct
       61 agaaggaaaa aaaatcgtca ttatgggcgt cgctaacaaa aaaagcattg cttggggttg
      121 tgctgaagca atgttgaaac aaggggcaga aattatctat acttaccaaa atgaacgaat
      181 gaaaaaatca ttagtcaaat tggtcggcga agaagcattc actgtagaat gtgatgtttc
      241 tagcgacgaa agcatcgaaa aagcaatgaa tgccatcggt gaatatgctg gggagatcca
      301 cggccttgtc catgcagttg cttacgccaa caaagaagaa ttgtccggaa atgtgtctga
      361 tatttccaga gacggctatc aattagctca agatatcagc agctactctt taattgctgt
      421 atcaaaatac gcacaaccgt ttcttgcacc aaacagcggg atcgttagca tgacttatct
      481 aggttcagaa cgcgcaatcc cgaattataa catgatgggt attgcgaaag cttctcttga
      541 agctgctatc cgttatttgg cagctgagtt ctcgcctaaa ggaattcggg tcaatggaat
      601 ctcagctggg gcaatcaaaa cattagctgt cacaggtgtt aaagactatc aaaaactgat
      661 ccagttatct gaatctcgta cgcctgatca gcaaggtgtg acgatcgaag aagtcggtaa
      721 tgcttgtgct ttcttaatca gcccactttc cagcggaatc atcggtgata ttatctacgt
      781 tgacaaaggt gtccatcttt cataaacttt tgttgcattc tttaaagtaa aacatataaa
      841 aatcaggaaa aaacttttta aagtctcttc ctgatttttt ctttttacgt ttttttcaac
      901 tcattacttt gaaattattc cacgacttca atccagcctt ctggtgcttc tatgtctcca
      961 aattgaatac ctgtcaattc atcatacaac cgttttgtta ctggtcctac ttccgtttca
     1021 ctataaaata catgaagatt gttattgtga taaatcccac cgattggaga aataacagcc
     1081 gctgtaccac atgcaccagc ttctgcaaat tgatctagat catcaatgaa aatatctcct
     1141 tcaattgctt gcagtcctag tcgttcttca gctaactgca acaaggaata tttcgtgata
     1201 cttggtaaga tagagggcga ttgcggagtg atgaattttc cgtcttttgt aattccaaag
     1261 aaatttgctg cacctacttc ttcgattttc gtatgtgtgg ctggatcaag ataaacacaa
     1321 tctgagtaat ccagttcttt tgcttctgtt cctggaagta aactcgctgc gtaatttccg
     1381 ccgacttttg ctgcaccagt ccccttagga gcagcacgat cgtaatcaga tacaataaaa
     1441 ttcgttggtt ttagtccacc tttgaaatac gctcctactg gcgtacagaa gacgacaaat
     1501 aaatattctg gagctggatg gacaccaatg ttttcaccga ccccaatcag taaagggcgc
     1561 aagtataatg tagcgcctgt tccatagggc ggcacaaaat cttcatttgc ttttaccact
     1621 tgcttgacag catcaacgaa catcttttca gggaccattg gcatcagcaa tcgtctagca
     1681 ctttgattca tccgtcgact attttgttca ggtcgaaaga gattgacctt gccgtctttt
     1741 cgacgataag cttttagacc ttcaaaacat tgctgaccat aatgaagcgc tggagagcct
     1801 tcatgaatcg tcaggtaatt atcttctgtc aattctcctt cttcccattg tccgtctttc
     1861 catttggcaa taaaacgcca aggagttttg atataagaaa aacctagatt gttccaatcg
     1921 atttgcatat acttaccacc ttcgttattt tctccacatt ttatcattat ccatattatt
     1981 tgtcatttta ttttcagctt ttttccttca ttatagttat ttttgctatt tccttttttg
     2041 aaaacttctt ggtacaatac agaaagaatc aaattttaga aaagaggatt ttatgttttc
     2101 attattagca actgccggta cgacagactc ttctggcgag acattgacag atcaagctgt
     2161 cagcaaagta aatgcgtttc agcggttttg ggacgggatt gattgggacg ccattttggc
     2221 tgcctttatc caaaaaagtt tatatctgat ttttttgatc gtcctttttt ggattcttca
     2281 ccgtgtggga aaatatctga ttgataagtc ttttaatcag tattctaaaa aagccattct
     2341 aaacgagagc cgcttaaaga ctttacatag tcttttaaat actatttttc attataccct
     2401 tggtttcttt tttatctatg caattttatc tgctatcggt gtgccgatcg gatcactttt
     2461 ggctggtgcc ggaattgcag gtgtggcaat cggtcttggt gcccaagggt tcatgagtga
     2521 tgttattacc ggatttttta ttatcatgga acaacagatg gatgttgggg actacatcaa
     2581 gctagcaaac ttaacgattg aaggaaccgt cgtatctgtt ggtatccgta cccttcagct
     2641 gaaagcaacg gatggtactg tccatttcat cccgaatcga aacatcacca cgatcagcaa
     2701 tctctctcgt gcaaatatgc aagtactttt agatattcgg attgttcctg aagaaggata
     2761 tgacaaaatc tatgagatca ttgatcgagt aaatcgtaca ttagctgaaa aataccaaga
     2821 agagctccaa actgaacctt ctatctttgg attagtggat cttggaaaca gcaattttgc
     2881 gattcgtacg gtctgctacg cattgaacgg aaaacaatat gccttaaaag aagagttctt
     2941 gagctcctat gtcaaagaat tgacagcttc tggtttcacg attccgaaca gtccaattgc
     3001 tgtcggaaaa taatttggac aaacgatgca acgaatctca ctaaaaagag cgtgggacaa
     3061 aagtaaagaa cacttttgtc ccacgctcta aacacgtata aacggcggaa gcagaagcaa
     3121 ctccttcgga aataagctga aattcacaaa aatttgaaaa g
//
LOCUS       DAZUMV010000193         3099 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000193 DAZUMV010000000
VERSION     DAZUMV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.201"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..728
                     /locus_tag="ACG8FK_002746"
     CDS             39..728
                     /locus_tag="ACG8FK_002746"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5098285.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            742..2244
                     /locus_tag="ACG8FK_002747"
     CDS             742..2244
                     /locus_tag="ACG8FK_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5098286.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2257..2733
                     /locus_tag="ACG8FK_002748"
     CDS             2257..2733
                     /locus_tag="ACG8FK_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5098287.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2830..2940)
                     /locus_tag="ACG8FK_002749"
                     /pseudo
     CDS             complement(2830..2940)
                     /locus_tag="ACG8FK_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1024 a          414 c          608 g         1053 t
ORIGIN      
        1 ttatccaaaa ataataaaaa attggagcgt ttaggataat ggataaaaag aggtttttag
       61 atcttatttc aaaaggtttg attgtttcat gtcaagcatt acctggtgaa ccattttaca
      121 aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct ggagcagttg
      181 gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta tcattaccaa
      241 tgattggaat tattaaaaga gattatccac cagaatctcc attcattact gccactatga
      301 gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat tgcactctta
      361 gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa gaaaaatatc
      421 ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat gcatataaag
      481 caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt aaaaaacaag
      541 atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt gtaattgctg
      601 agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga gttgcaggta
      661 ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt gttaaagcat
      721 taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg cgctttcatg
      781 ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg agcgttaaca
      841 aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca tacaatttta
      901 agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat ttttgcaatt
      961 ggtattgctg taggacttgc aaatggagat aaaggaacag ctggtttagc tggtggagta
     1021 gcttatttag tatatactgc aactattagt gggtttcttg aacttttttc agcaaaagat
     1081 gcaacactag atacaggtgt agtgggatca attgttgttg gtggtactgt tgcattttta
     1141 cacaatcgtt atagaaaaat tgaattacca caattcttag gtttttttgg aggatcaaga
     1201 tttattccga ttgtttctag tttagctgca atcattattg gttctttctt ttatttaatt
     1261 tggccaccaa ttcaaaatgg tttagtagtt gctggtgaac aaattgccca aatgggtagt
     1321 ttaggaactt ttttatatgg ttttttattg cgtttaacag gtgctgttgg actacaccac
     1381 accatttacc ctttgttctg gtatacatct ttaggtggta ccgaaactgt agctggatca
     1441 acaattgctg gagcccaaaa tattttcttt gcccaactag ctgatccaaa ccacactggt
     1501 ctgtttactt atggtactag attttttgca ggaagatttg caacaatgat gtttgggtta
     1561 ccagcagcat gttatgcaat gtatcgtgca attcctaaga aaaatcgtaa aaagaatggt
     1621 gggctttatt ttagcggggc ccttacatca tttttaactg gaattactga accagttgaa
     1681 tatatgtttt tatttgttgc tccttggctt tatgtgattc atgcattctt agatggatta
     1741 tctttttatt ttgctgatat cttaaatatt cgtgtaggga attccttttc aggtggtttg
     1801 atcgactttt tattattcgg tgtacttcaa gggaatgaca aaactaattg gttaaaatta
     1861 attccttttg gtattgcatg ggcatttata tatttctttg tttttacatt ctttatcaaa
     1921 aaatttaagg tagctattcc tggaatggaa gaagaatcag aagttcattc tgaaaaagta
     1981 ataaacaaaa atacatcatt acatgaagag tctcttatca ttattgaagc tcttggtgga
     2041 aaagaaaata ttgagtctgt tgaagcttgt gcaactcgtt tgcgtgttgc tgttacgaat
     2101 ggggagatta ttcaaaaaca aacaataaaa gacactggtg caactgctgt cttcgaagtg
     2161 aagggtggca tccaagctgt ttttggaggg aaagctgact tattaagtca agaaatcaat
     2221 caaatattag gaaaagataa ttaggaggtt tttaatatgt ttgaaatttt caaaaagaaa
     2281 aaacaagaaa ctttttatgc accttgttcc ggaaaaatca tttccatcac agaagtaaat
     2341 gatcctgtct tttcacaaaa attactaggt gatggttttg ccgtcatacc tgatggttca
     2401 aaaatttatg ctccattggc tggtcgagta acaaatatat ttcctactaa acatgctatt
     2461 aatataaaaa gcaactcagg cattgattat ttgattcata ttggaattga tacagttgaa
     2521 ttacaaggag agcctttttc gatttgtgtt caagaaaatg atgaggttac tcctgaaacc
     2581 tcgttggttg atgtggattt tgacaaaata ataaaatctg gtaaagatac ttctgttata
     2641 gttgtttttc cggagaaaaa tcagttcaat gatttaataa tagataatgg tcgaattgct
     2701 catgggaatt catgtggaaa aatcactttt taaaaaagtg tatataaaaa gctataaaat
     2761 aagctaaata ggatatttgt caaatgaagt tggtgaacag aaagaactgt tttctttctg
     2821 ttcatttttt tattttatta gtaatgattc cccatgccaa atcatgatcc gtaatttaaa
     2881 gttataaaag cttcgaaatc cattggcatt tcgttggaat accttgatat gattattcaa
     2941 atataaaata tagagagaag agagagaggg aagatattta taaagggcaa agttatgttt
     3001 actcaactta ggaaatagaa atagatcaat ttatattctc ttgtcaatcg aatatgaacc
     3061 ttagcactat ataccaaata tagtaaaatg ctgaaagat
//
LOCUS       DAZUMV010000194         3071 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000194 DAZUMV010000000
VERSION     DAZUMV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.202"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..1102
                     /locus_tag="ACG8FK_002750"
     CDS             287..1102
                     /locus_tag="ACG8FK_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098288.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1228..1485
                     /locus_tag="ACG8FK_002751"
     CDS             1228..1485
                     /locus_tag="ACG8FK_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098289.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1737..2309)
                     /locus_tag="ACG8FK_002752"
     CDS             complement(1737..2309)
                     /locus_tag="ACG8FK_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5098290.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2325..2930)
                     /gene="fic"
                     /locus_tag="ACG8FK_002753"
     CDS             complement(2325..2930)
                     /gene="fic"
                     /locus_tag="ACG8FK_002753"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS5098291.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1018 a          539 c          473 g         1041 t
ORIGIN      
        1 aaataagaaa ttcaaaaaag aaaaaacttt ccttaaaaac aaaaaatagg agtattcagt
       61 ttcttaatag ttttgtaata gttgtatgta gaagttttct taatagtttt tgtaataatt
      121 tattaatcgt agtttatata acttatttgt aagtgactag agaattaaat aaaaaataat
      181 tactatctag tacgaaatta attacatagg ataatatatt aaggtgctgg actacaagta
      241 aaaaatactg tatgatcatg acagattcaa gaaaggagca ataagattgg ttaagatagc
      301 aggactttct aaaggtggaa aaaccgtttc acaactttat aaacaaaact acagagactg
      361 gagacagttc agaaaaaaca ttaaggattc ttactttatt ctaccaacag aacttgaaaa
      421 atatttaccg aataacaaag ctattaattt atatctgtat tactgtttta aggcaaagaa
      481 tgaaggcggg gattcctggc attcggtcaa taccatagca gagtctctag gagtgacaac
      541 taaaactata aacacctgga ataaaacctt agttgattta ggtatgattg cacgtataga
      601 cgataaccat ctcagtaaat cgacaatttt acttcctctc tctagttact ataaattagc
      661 ccttgatagt tctttaaagg atgttaccga gagcactgtc catgaaattg atggcaactt
      721 ggttgcagtt tatcatttgt ttgaatggag aaaaaatgag gctgatgaaa attataacgt
      781 tccttataac acactctgtt gtgtttttaa aagaaaaaca caaaaagata ctatctataa
      841 atttatcttg gtccaagatg agaaaataag taatttcgaa ttaagcactc cttctagtaa
      901 gatttatgac gatgcatata gatttgaatg tcaagatcta gaagccctcc tttccgcaaa
      961 taatctaaac aatatagaac atgaaaactt tgttgtaaac actagattta atttagtttc
     1021 tgaaaaggac tcagatttgt tagatttgct gatagagctt actaaaaatc tagataagaa
     1081 agaaaacatt actgtactat aactagcatc ggtccatcat taagtttgga ctattagaac
     1141 acttgctatt acagaaaaag gtatgctagc cacgcttact atttattggt atcaaacaag
     1201 tatcttaatt tgaaaggaag atgatttatg gctaatttat atatagagaa caaaggagac
     1261 ttgaatgcca ttattacttt aatcgtagtt atggtaaaat caataggtaa tatcacaata
     1321 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
     1381 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
     1441 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
     1501 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
     1561 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
     1621 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
     1681 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1741 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1801 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1861 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1921 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1981 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     2041 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     2101 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     2161 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     2221 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     2281 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     2341 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     2401 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     2461 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     2521 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     2581 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     2641 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     2701 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2761 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2821 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2881 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2941 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     3001 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     3061 gcacagtcta g
//
LOCUS       DAZUMV010000195         3054 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000195 DAZUMV010000000
VERSION     DAZUMV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.203"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACG8FK_002754"
     CDS             <1..227
                     /locus_tag="ACG8FK_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098292.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="ACG8FK_002755"
                     /pseudo
     CDS             227..1620
                     /locus_tag="ACG8FK_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="ACG8FK_002756"
     CDS             1635..1841
                     /locus_tag="ACG8FK_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098293.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="ACG8FK_002757"
     CDS             1862..2458
                     /locus_tag="ACG8FK_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFS5098294.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1116 a          410 c          594 g          934 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttgg
//
LOCUS       DAZUMV010000196         3026 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000196 DAZUMV010000000
VERSION     DAZUMV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.204"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..446)
                     /locus_tag="ACG8FK_002758"
     CDS             complement(78..446)
                     /locus_tag="ACG8FK_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS5098295.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            596..1078
                     /locus_tag="ACG8FK_002759"
     CDS             596..1078
                     /locus_tag="ACG8FK_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS5098296.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1115..2125
                     /locus_tag="ACG8FK_002760"
     CDS             1115..2125
                     /locus_tag="ACG8FK_002760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS5098297.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2240..2953)
                     /locus_tag="ACG8FK_002761"
     CDS             complement(2240..2953)
                     /locus_tag="ACG8FK_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098298.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1015 a          493 c          596 g          922 t
ORIGIN      
        1 aaggatagga ttgaacaaaa cctgtataag ctgatgaata gtttcctcgc cagtaaaagg
       61 tttattcgta taaagcttta tcgccaaaat ttatcatgac cttttttatc ttcaatatta
      121 aatgaaatca attgttcaat caattcccag ttgattacac ttttccaagg aactttgatg
      181 acgttatccg tatatacgta gcctgcttct tggatatttt tttcaagggc acgaatcgct
      241 gctttttccg gtgcgataga aaaatgattt ttagcaaaac taaatgcaat aataaaggta
      301 ccatggtcag taaacatagg ttggttccat tttacagttg tgtctagctg gggatacgtt
      361 tctgttatct tagcaaaaag gtctttgatc ttttgttgat gttctttttc tttgatctga
      421 ttgatgatcg gctgaaattt ttccattcta aaacgcctcc attaagtggt ttgatttatt
      481 ttaccagaaa aacaatagtt atgtataaaa aacgaacaaa acacaagtaa tgttgtaacc
      541 gctatattta tatgataaga tgtctcaaat aataaaatag aaaagagaca taaaaatgat
      601 tgacttggca aagaaattct tacacgaaaa tatatatttt gagttgtatc atcatcgagc
      661 catttataca aatgaagatg ctttggtaat aaagaaagaa caaggattta caggtacaga
      721 gaccaaaagc ttattcttaa aagataaaaa agataataac tacgtatatc tgacttttac
      781 tacgaaacaa acggatttca aagcattagg gaagatcgtc ggtaaaaggc tttctattgt
      841 ttcatcagaa cgcatggaag aacaaacggg acaaaagcag ggcgctgttt ctccgtttgg
      901 ttatgaaaaa cagattccga ttattattga tgaagagctc ttatcaaatg aaaaacttgt
      961 ttttgcacca ggtagaccag atcaaacgat ggtcatccaa acggttgact taaacaaaat
     1021 catccagcta cttgagatgc cgatattttt gatgccggca aacgaaaaaa acaattaacc
     1081 aagtaaaata gtgcatactt ggaggtgaag caacatgata agttatccat tattagaaag
     1141 aaatgcaacg attggggtta ctgcaccatc ctcaggcgta tcagaggaac accattcgtt
     1201 actcgaatca gctttaaaga gaatggaaga aaaatctttc caagtagtta ctggaaagac
     1261 gctttggacg caagataagg aaaaatcagg tccagcaaga aaacgagcag atgaattcaa
     1321 tcaaatgatg caagatgaaa atatccaaat gatctttccg ccttggggag gagaattatt
     1381 gattgagatt ctcgattttg tcgattttga tgctctacaa ccaaaatgga tagtaggtta
     1441 ttcagatatt agtacgttgc tacttgctgt cacattaaaa acagggatag ccactgcaca
     1501 tggaaccaac cttattgatt taagaggaga gtacttggat gaaacgacag caatgtggga
     1561 aagtgtactg aaaacaagag aaggagaatc gattctacag caaccttctg aaaagtatca
     1621 aaaagagtgg ccccatgagc attcatcatc ttctgttttc catctcactg aaccaacaaa
     1681 ttggcaatca gtcagcggag atgcagtaag gatgaaggga cgattattag gtggctgtat
     1741 tgatacgatc agtcatttaa tcggtacccc ttatggagat gtccaatctt ttaaaagaaa
     1801 gtataatgat ggaaagccga tcttgtggtt tctagaaaat tgtgagctat cgtcagttga
     1861 tttaagaaga tcacttgttc agatgaaact ggcaggatgg tttgatggca gctcggggat
     1921 catgttcggt agaagtgcag cagaggattt gttagattat aaaatgaagg atgtatatga
     1981 agatctgtca aaggaattaa aactaccgat cttgtatgat attgattgtg gtcatgtacc
     2041 gccacaactg acattgatca atggtgccta tgcagaagta gcggtagaag atagaaaagc
     2101 gactgttttg caaacgttta tttaaaaaat tttttcctgc cttggagttt atcaaagacc
     2161 aacaaatcta aatagaaaag aaaatgaaag aagctgacag tattttgcca gcttcttttt
     2221 gtatgatcta ttagaaattt taattatcca taactatcac attgatcagc tgtttcacat
     2281 catttggatt acctgtagga atagctacta cagatgcatt atcaataatt tcttgagttg
     2341 tttctctaat gaacgctgga taagatgttt gatctgcagg catttgccaa tgtgaaggaa
     2401 ctggataact agtgattgtc ccgtcatggt tacttgaata cactaaaact ccctggtaac
     2461 tgtatttccc agtgagcata accacatctt tcggatagac tgcactccct gcatcataag
     2521 gggcgatagg tgttcctgca ggataatgaa taacatgtag ttcgaaatca cccgatgtat
     2581 ctctaaaagt attacctcta attgctagcc aaacacgtgc gtactcaatt tgtagatcag
     2641 aatatcctgc taacggatct attgactgag aagactgtga ggaagttgtg tctattgttg
     2701 aagaagaaat atttgtctca actgtactac tagatgtaga agaagtattg ggctctgaga
     2761 ctttactgct tgttgaagat gtattcgttt cagtagaagc tgatttctga gtacttgatt
     2821 tttcagctga actggtttgt ttgattgaac ttttgtctgt ggtcgcagat ggatcactat
     2881 tttctttact aggagacgta catcctacaa ggaataaaga gaccaacaat aaaataaaac
     2941 ctaacttttt catataaaat acctcattat ttttttaata ccagtatagc ataatcctga
     3001 atttttgaat ccgtaaaacc ttgtct
//
LOCUS       DAZUMV010000197         2996 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000197 DAZUMV010000000
VERSION     DAZUMV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.205"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..927
                     /locus_tag="ACG8FK_002762"
     CDS             52..927
                     /locus_tag="ACG8FK_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS5098299.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1072..1224)
                     /locus_tag="ACG8FK_002763"
                     /pseudo
     CDS             complement(1072..1224)
                     /locus_tag="ACG8FK_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1492..2823)
                     /locus_tag="ACG8FK_002764"
     CDS             complement(1492..2823)
                     /locus_tag="ACG8FK_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5098300.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         1057 a          544 c          458 g          937 t
ORIGIN      
        1 aacgtttctt tatactaaat ataaataagt gtttcatgaa aggagaacta gatgaataat
       61 tggcttgagt attttcctga aaacgttttg gaacgcggtt acagctatca tctgcacggt
      121 tttgtccgtc atttgaacta tacaagcaag tacttgtccg caacagtttc tggcacggaa
      181 gattataagg tagtgatcac ttgggatgaa aagaccaaca tgacttgtga ttgtctatat
      241 gccatagaag gaaaaaaatg caaacacatg gctgctgtct tattcgctta cgaagaacgt
      301 ccaataaaaa aatccaacta ctcactttcc gaactctcgt cccttgtttc ctccgctagt
      361 tcctctcttg tacgggagct tttaacagag attcttatag aacatccgca atttatcgaa
      421 cgcttcaaag ttaagatgcc atttcatgca ataaattatt cagataaatt aacaactatc
      481 atccataaat acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc
      541 atggagatgc gaaagttcat ccaagaagca gttgaatcgc tcattcagca aaatgcctat
      601 cttcccgcat tcgaattgat caatgaagtg attgctactc tcgaaacatt ctattgggag
      661 cctgaggatg aacgaaccct tcttctgata gaggactgtt attatttatg gaaagaatta
      721 ttagcagaag cacctcatgc agaaaaaagg caaatgttct cttggttcgt ctgccaagtc
      781 gatcatactg acgcctctta ttcaaaacgc tactccataa aaatcctgaa agaagacttt
      841 cgggaaaaag aattttctaa ccaaaagaaa aaaatagata aaaaaactaa agaaacggta
      901 aagaaagacg atttcaatga aaaatagcaa gtaaacgata acaaccatcc taaatccaca
      961 agaaatacag cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa gtaaagaaga
     1021 cttaacgcgt acccaatatt agtcatttga ctattaggta cgcgttttgt tttataagtc
     1081 atgacaaatt tccttcgttg ctgggtagat tttccggcat tgttgataga ttttatgata
     1141 cttttctaca ttttgtaaga gaggttccac tgcatctgta tattggataa aattctgaca
     1201 acaactttca ggtgtgtcga accactctaa tctttaaaga taaatattaa aataacgatt
     1261 aataatatta acagtcttta tttttcacca ccaaaaacct atctttgcat atactaaata
     1321 gacgtatata cacagatagg tttgtcttgt gaaaaataat gattatcctt accaaaaaat
     1381 gtttgttttt ctacatgttc gtacttatca aaaaatatga tccttattga aattactggc
     1441 tatttatatt ccaacgactt cttgtgttaa tttttgttat ctcatagtaa attatgtttc
     1501 attttcactt aatttagttg tcacgtgttc gtcaattcgt aaaaacggta aatatataaa
     1561 cacacttgcg gctataatta atatttgtaa aacagctgct cgccagtctc cggctgttgc
     1621 taaaaatccc gatataactg gtggcgtagt ccaaggaatt tgcactacta catgattaat
     1681 taatcccaca gcagttgcaa aatatgctac tgtcaaagaa aaaatcggtg caagcacaaa
     1741 gggaataatc agactgatat taaatacaat aggcaatcca aaaataatag gttcgcttat
     1801 attgaatata gcgggagtta ccgataattt agcaaagtcc ttataatctt ttctacgact
     1861 gaagataaat attgcaatca ataaagcaat cgtatttccc gatccaccca ttaccgcaaa
     1921 ggtatcttta aaggccatat tgataatatg tggaatttgc tctttgtttg cataagctgc
     1981 catattttct tgcatattct gaatcaaaaa cggatctaac aatgaacctg atattactgc
     2041 ttgatggata ccaaatccaa aaaatagatt agcaatagat gtaattaaca aaaatcctgg
     2101 taaaccagtt gtaattttac ttaatggctc tgtaactatc gtggtaatta gcttatttat
     2161 atccatatta aacaattgaa tgattataaa tgacaataaa gcaaacgagg taatcgtaat
     2221 aatgattggg attaatacat taaaagactt taaaacagcg ggaggaatat ttccggatag
     2281 ctgaatctgt agtttcttat tattagaaag tttaataaat aattctgtag ccgctaatcc
     2341 aacaaatata cctacaaaca ttcctgaagc tcctgtatag gatataggta tcacacccgt
     2401 cacttcaaca gctttatccg cattttcagg agtaaacatc atctgcatag gtgttaatac
     2461 aacaaatgtc gaaatagcaa ctaataccgc tgcaatatga tttgtataat ttctgcttgc
     2521 acacatatga taagatactg ctgctacaac taataatgta gttatgctta atgtaccatt
     2581 ggcaattgaa gtaccaattt gttgtaacgt ggtcaatgta ctagtatcga caatttcccc
     2641 catgaatcct gttggttcta agatcactga attaataagt gtcacaaatc ctgctaacat
     2701 cataaatggc ataaatgtag caaacgcatc tcgcaaagtt cttaaatgga tctgattccc
     2761 caacttagtc gcaactatac caaatttttc aaatacaatg tccttcctgc tacttttgcc
     2821 catatttttt ctcctttatg cttattgtaa tcgattactt tttccaccca aataatacat
     2881 cacttatcaa aaaaagtaaa tacttttaat taaatatata tatctttttt agtataaaaa
     2941 aataagctct gaaaaaataa ttttcccgag cttatttttc aacaaaaaca gcttca
//
LOCUS       DAZUMV010000198         2992 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000198 DAZUMV010000000
VERSION     DAZUMV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.206"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..1053)
                     /locus_tag="ACG8FK_002765"
     CDS             complement(106..1053)
                     /locus_tag="ACG8FK_002765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5098301.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="ACG8FK_002766"
     CDS             1299..1763
                     /locus_tag="ACG8FK_002766"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS5098302.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="ACG8FK_002767"
     CDS             complement(1825..2868)
                     /locus_tag="ACG8FK_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS5098303.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          990 a          593 c          571 g          838 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DAZUMV010000199         2947 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000199 DAZUMV010000000
VERSION     DAZUMV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.207"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(449..865)
                     /locus_tag="ACG8FK_002768"
     CDS             complement(449..865)
                     /locus_tag="ACG8FK_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098304.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="ACG8FK_002769"
     CDS             complement(867..1148)
                     /locus_tag="ACG8FK_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098305.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="ACG8FK_002770"
     CDS             1948..2922
                     /locus_tag="ACG8FK_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5098306.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DAZUMV010000200         2932 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000200 DAZUMV010000000
VERSION     DAZUMV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.208"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..984
                     /locus_tag="ACG8FK_002771"
     CDS             88..984
                     /locus_tag="ACG8FK_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5098307.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1076..1291
                     /locus_tag="ACG8FK_002772"
     CDS             1076..1291
                     /locus_tag="ACG8FK_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS5098308.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1309..1581
                     /locus_tag="ACG8FK_002773"
     CDS             1309..1581
                     /locus_tag="ACG8FK_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5098309.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1583..2446
                     /locus_tag="ACG8FK_002774"
     CDS             1583..2446
                     /locus_tag="ACG8FK_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5098310.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1163 a          464 c          510 g          795 t
ORIGIN      
        1 aaagagggct tttatatatt aatcacaaat cacttatcac aaatcacaag tgattaatca
       61 caaatcactt gtgatttgtg attcttaatg atacaatatt actatacaaa aaaagaatgg
      121 ggcgtagtta tggagaagga aaaactaaaa atacttgaag aattaagacg tattttaaac
      181 aataaaaatg aagcaattat tatcttgaat aattacttta aaggtggtgt tggaaagtcc
      241 aaattatcga ctatgtttgc ttacttgaca gacaaattga atttaaaagt tttaatgatc
      301 gataaggact tacaggcaac attgacaaaa gacttagcaa aaacttttga ggtagaattg
      361 ccacgtgtca atttttatga aggcttgaaa aatggaaact tggcttcttc tattgttcat
      421 ttgactgata atttagactt gatccctggc acgtttgatt tgatgttact gccaaaatta
      481 actcgctcat ggacttttga aaatgaaagt agattgcttg ctactctttt agcaccttta
      541 aaaagtgact atgatctcat tattattgat actgtaccaa cgccaagcgt ttatacaaat
      601 aatgcaatcg tggcgagtga ttacgttatg atccctttac aagcagaaga agaaagtaca
      661 aacaacattc aaaactatat ttcctatttg attgatttac aagaacaatt taaccctgga
      721 ctagatatga tcggttttgt tccttattta gttgatacgg acagcgcaac gataaaatca
      781 aacctggaag aactgtacaa gcaacataaa gaagataact tggttttcca aaatattatc
      841 aagcgaagta ataaagtaag tacctggtct aaaaatggaa ttacagaaca caaaggctat
      901 gacaaaaaag ttttatccat gtatgagaac gtattttttg aaatgcttga gcgaattatt
      961 caattagaaa acgaaaaaga atagaatcac aaatcacaag tgattaatca caaatcactt
     1021 gtgatttgtg attgttgatg ataaaataag aataagaaga aatagaaaga agtgagtgat
     1081 tgtgggaaat ttaggcgcac aaaaagcaaa acgaaatgat acaccaatca gtgcaaaaaa
     1141 agatataatg ggagataaga cggttcgtgt tcgtgctgac ttacaccata ttataaaaat
     1201 tgaaacagca aaaaatggcg gaaacgtaaa agaagttatg gatcaagcct tagaagaata
     1261 tatacggaaa tatttacctg ataaacttta aaaaggagtg aaaatgaaat ggcagttacg
     1321 tatgaaaaaa catttgaaat agagatcatt aacgaattat cggcaagcgt ttataatcga
     1381 gtattaaact atgttttgaa ccatgaacta gatactaaaa atactcgttt actagaagtg
     1441 aatcttttaa atcaattaga agtggcacaa gaagttgatt tatttcaaca accatttgaa
     1501 gaattacaag ctattcatga gtattggcgg tcaatgaatc aatattcaaa acaaattttg
     1561 acaaaagaga aagtggctta acatggcgaa tattactgac ttcaccgaaa agcaatttga
     1621 agatcgttta gaaaagaatg ttgaacgact aactaaaaat agactagcgg ttgaatcgcc
     1681 aaccgctttt ttacttggtg ggcaaccagg atcaggtaaa actagtttgc gatcggcaat
     1741 ttccgaagag acacaaggaa atgttgttat cattgataat gatacgttca aacaacagca
     1801 ccctaatttt gatgaactag tgaaacttta tgaaaaagac gtagtaaaat acgttacccc
     1861 ttattctaat cgcatgacag aagcgatcat aagccgtttg agagataaag ggtataattt
     1921 agtgattgaa ggtacaggac gaacaacaga cgttcctatt caaaccgcaa caatgcttca
     1981 agccaaagat tatgaaacaa aaatgtatgt catggcagta cctaaaatca actcatattt
     2041 aggaacaatt gaacggtatg aaactatgta tgcagatgat ccaatgacag ccagggcaac
     2101 accaaaacaa gcgcatgata ttgttgtcaa aaacttaccg accaatttag aaacccttca
     2161 taaaacgggc ttatttagcg atataaggct ttacaataga gaaggagtga aactctattc
     2221 aagcttagaa acgccttcca ttagtccgaa agagacctta gaaagagaat taaatcgtaa
     2281 agtatcaggg aaagaaattc aaccgacttt ggagagaata gagcaaaaaa tggttcaaaa
     2341 tcaacaccaa gaaacccctg aatttaaagc aattcaacaa aaaatggaaa gcttgcagcc
     2401 acctacacca ccaataccca aaacacctaa acttccaggg atttaaacct taaaaaagta
     2461 aaagagtagt taccaaaaaa cggtaactac tcttttttta taaaaacatt tcctcatttt
     2521 ttaaaagctg atcgagaaat aaagcacaca actgtattct ttcttttttc agttggtaca
     2581 ttttgcagtc tgtaacgtgc catttactgt ttgttacctt aacatatcca gtcgaaaaca
     2641 caagggtata taaacgagca agctcgccct tgtgttttct ttgtgtctat gttgcgttct
     2701 cgtcacagca aacgacacga acacactggc gaaaatgaac caacaaaacg aaaaaagaaa
     2761 ggagcgtaca ctgtaattta tttctagctt ttaaaacatg aggaaaacag cttcacttcc
     2821 tcaaacgcct aacaaaaatg gttgtcaaaa aactaaaaat caaaaaaaga aaagaggaaa
     2881 agaaaatgga aaaaacaaaa aaattacaat tagaagattt tacagaaaat gg
//
LOCUS       DAZUMV010000201         2873 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000201 DAZUMV010000000
VERSION     DAZUMV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.209"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..124
                     /locus_tag="ACG8FK_002775"
     CDS             <1..124
                     /locus_tag="ACG8FK_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5098311.1"
                     /translation="TFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(210..1304)
                     /locus_tag="ACG8FK_002776"
     CDS             complement(210..1304)
                     /locus_tag="ACG8FK_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS5098312.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1493..2254
                     /locus_tag="ACG8FK_002777"
     CDS             1493..2254
                     /locus_tag="ACG8FK_002777"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5098313.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2369..2809)
                     /gene="fabZ"
                     /locus_tag="ACG8FK_002778"
     CDS             complement(2369..2809)
                     /gene="fabZ"
                     /locus_tag="ACG8FK_002778"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5098314.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          918 a          572 c          481 g          902 t
ORIGIN      
        1 gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat cattgaatta
       61 cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt ctagcttaat
      121 ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact ggctatttct
      181 agcggattat ttctgcaata actaattatc tattttttat tttcaattaa tttaaagctt
      241 gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct tcctacaaca
      301 ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc tttgtattga
      361 ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt attatgaggc
      421 gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat atattctgag
      481 cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt ttctgtaata
      541 ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc tgccatttct
      601 ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat caatcctgta
      661 aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac
      721 aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac gaatgccatc
      781 gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa
      841 ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt gtttggcgaa
      901 taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc gattttgata
      961 ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat atctaagcgg
     1021 accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat ctccaactgt
     1081 tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt cgcgtaatca
     1141 actacttctt taaattctga aagaactttt tctttatcat cattgatctg cagcaaactg
     1201 gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt gtccttttca
     1261 aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc atttcctcct
     1321 gcatcttttc gtttttgtat ccacttacat tatagcaaac acaaagtaaa tggtctacac
     1381 cttttctttt tcttttaaaa taaatgataa attccaataa tttaagtagg tattttccct
     1441 ctattttgtt atcattaaag ataacaaaat agtaaattgg aaaggaacag ttatgaagaa
     1501 aaataaagtt attttaagta ttagtttagc agcagcattg ctgtttgctg ctggttgttc
     1561 tgcaaacaac acgacttctt ctagttcgtc tacagaaagt tcaaaaagta catctactgc
     1621 ggaaagcacg actgccagta gcgtcgattt gaacgcattg gaattaccac aattgacgaa
     1681 agatgtcgcc gaaaatgaag atctggtcga aatggtgacg aataaaggca caatcaaaat
     1741 caaattattt cccgaacacg caccaaaagc ggttgaaaac tttatgacac atgcaaaaga
     1801 cggttactac aacggactga ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg
     1861 cgatcctaaa ggcgatggca ctggagggga aagcatttgg ggagaaggat ttgaaacaga
     1921 gatcaacaac cacctttata atataagagg tgcgttatct atggctcgct cacaagatcc
     1981 taacagtaac ggcagccagt tctttatcgt tcaaaataca gatgatatgc atgatggtct
     2041 tttaaaagac gactatcctc aagcaatcat cgacgcttat aaaaacggcg gttatccttc
     2101 actagatggc cattatactg tcttcggcca agtgattgaa ggaatggatg tcgtggatgc
     2161 tattgcaaat atcgaaacag atagttcaga caaaccaaaa gaagaagtaa aaatcgataa
     2221 aatcaatata ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag
     2281 atgacaacgt atgctaggat tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc
     2341 ttttgttttg ctatatcagg ttttatagtc aagcattttg gttgacgatg aacgtgaact
     2401 cgcactcaca gactttttta tcatctacat atgctgcaga taacgctgta ccgactgatc
     2461 ccttcatttt ggtgatttca aaatgacatt tcagcacatc tcctggaaca actttttgac
     2521 ggaatttggc cttattgatc ccaccgatat aagctctttt tcctagaaac tcttctgatt
     2581 ttaggataag tatcgaacct gcttgtgcca gtgtttctag aatcaacgca cccggcatca
     2641 ctggattgtc aggaaagtga ccttggaaaa aatcttcatt gattgttaca ttttttgtcg
     2701 caacgatctt ttttcctggt tctaaagtat ctacataatc aatataacaa ataggataac
     2761 gattaggaat caattccatt acttcagtag cagataatac gtaagacata attaaactcc
     2821 ttctttcagg caattgccat tttcattact ttgactatca aagtatcata ttc
//
LOCUS       DAZUMV010000202         2752 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000202 DAZUMV010000000
VERSION     DAZUMV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.210"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      103..277
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            415..2007
                     /locus_tag="ACG8FK_002779"
     CDS             415..2007
                     /locus_tag="ACG8FK_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5098315.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            2020..>2752
                     /locus_tag="ACG8FK_002780"
     CDS             2020..>2752
                     /locus_tag="ACG8FK_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5098316.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRIL"
BASE COUNT          866 a          517 c          603 g          766 t
ORIGIN      
        1 tttatcagta ttttttcaaa aaccagcagt tatagattga caaagcctct cgagtatcgt
       61 aaactttttt tgtataaata actgaatacg agatgcctca atcaatgagg tagaggtcgc
      121 gagaatcatt aaccctgtgg agtgaagcaa acatggagaa gcaggaatag ggaggatcgc
      181 cgaaatgtgc acttttttgc ttggagtgta tgttgggacg caagttaaga gcttgcggac
      241 tgtcttagca gtgatgctaa gttgcgctat gttttgtgag aggtttatca ccttatggaa
      301 ttttagcggc ctttttatat ctataaatcg gtcgcttttt attttttcag ataaagcttt
      361 accgcatttc atctagaccg ctcgtgtatc atataatcag aaagggagaa aaaaatgaaa
      421 aaaaatatgg tttcagttgc cgtactattt gcttttgtct tctcactatt cagtgtggga
      481 tctctggcaa atgcagatga aaaaacaccg agcgatgagt ttcgcgtagg tatggaggct
      541 gggtatgcac ccttcaactg gtctcaacaa acagatgaga ataatgcctt gccaattcaa
      601 ggccaaaata gttacgccgg cggttatgat gttcaaatcg ctaaaaaagt agcagatggt
      661 ttagggaaaa aattagtcat cgtccaaacg aaatgggatg gtctggctcc tgcccttcaa
      721 tcaggcaaga tcgacgcgat catcgccgga atgagcccta ctgctgaacg acgaaaagaa
      781 attgatttta ccgatcctta ttacgaatca caattagtag tagtagttca aaaagatagc
      841 aaatatgcaa aagcaaaaag tttagcagat ctttctggag caaagatcac tgctcagtta
      901 aatacattcc actatactgt gatcgatcag ataccagatg tgcagaaaca acaagcaatg
      961 gataatttct cagcaatgag aacagcttta gcttctggaa tgatcgatgg ttatgtcagc
     1021 gaacgtccgg aaggtgtaac ggcaactagt gtgaacaaag acttggaaat gcttgaattc
     1081 agcggtgcag atggcttcca gacaaacccg gaagatgttc agatttctgt tggtatgcgt
     1141 aaaggcgacc cggatatcca aaaagtaaac cagattcttt ctggcatctc taacgatgaa
     1201 cgaaccaaaa ttatggacca agcagtcaaa gatcagcctg ctgcaactga tagtgaagaa
     1261 aaacctggca tcatttctga tttcactacg atttggaaac aatacggcaa tatgttctta
     1321 cgaggtgctg gacttacctt gttcatcgca ttgatcggga cagttgtcgg tactacactt
     1381 ggattactga ttggtgtctt ccgttcggta cctgaatcag gaaatgcact gactcgcttc
     1441 ttccaaaaaa tcggaaatgc acttctttct atctatgtcg aagtattccg cggaacaccg
     1501 atgatggtac aagcaatggt catcttctac ggtctggcac tcgcttttgg catttcattg
     1561 aaccgaacga ttgccgcttt gtttattgtc tctctcaata ctggagctta catgacagag
     1621 atcgttcgtg gggggatttt tgcagtagat gaagggcagt tcgaagctgc gcaagccatt
     1681 ggtatgaccc atggccaaac gatgagaaaa gttgttattc cacaagtttt acggaatatc
     1741 ttgccagcaa caggaaatga gtttgttatc aatatcaaag atacagcagt tttaagtgta
     1801 atcggcgtag ctgatttgtt cttccaagga aactctgctt ccggtgcgaa tttccaattc
     1861 ttccaaacat ttacgatcgt cgggatcatc tacttagtca tgacctttac gatcaccaga
     1921 atcctgcggg cggtagaaaa gaaaatcgat ggtccttcag cttataccaa gcttgaagaa
     1981 atcgacaatt caaatttgca ggaataagga ggaagaatca tgaatgaaaa aattattgaa
     2041 atcaaacatc taagaaaaag cttcggtgaa aatgaagtat tgaaagatat cagcgtaaca
     2101 gtcaataaag gtgaagttgt gacgatcatc ggttcttccg gttctggtaa atctactttc
     2161 ttgcgctgta tcaatttact ggaaaaacca acaggcggcg agatcattta taatggagaa
     2221 aatgtattag ctcctaaata taatttacct aaatatcgaa cgaatctagg tatggttttc
     2281 caatccttca acctgttcaa caatatgaat gtcttggaaa actgtatgtc tggtcaaaca
     2341 actgtcctga aacgagataa agaaactgcc aaaaaagtag caatcgaaaa tctggaaaaa
     2401 gttggtatgg atcgttatat cgaagccaaa ccagcacaat tatctggtgg acagaaacag
     2461 cgtgtcgcaa ttgctcgggc attatcaatg aatccagatg tattgctatt cgacgaacct
     2521 acttcggcgt tagaccctga aatggttggc gaagtgctta atacgatcaa agacttagca
     2581 catactggct tgacaatgct tatcgtgacg catgagatgg aatttgcaaa agatgtatct
     2641 gaccgtgtca tttttatgga taaaggtgtg attgcagaag aaggtacacc agaagatatc
     2701 tttgtccatc cgaaagaaga acgtacaaaa gaattccttt cacgtatttt aa
//
LOCUS       DAZUMV010000203         2719 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000203 DAZUMV010000000
VERSION     DAZUMV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.212"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACG8FK_002781"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACG8FK_002781"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5098317.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DAZUMV010000204         2559 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000204 DAZUMV010000000
VERSION     DAZUMV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.213"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..721
                     /locus_tag="ACG8FK_002782"
     CDS             65..721
                     /locus_tag="ACG8FK_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5098318.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            721..2097
                     /locus_tag="ACG8FK_002783"
     CDS             721..2097
                     /locus_tag="ACG8FK_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5098319.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            2227..>2559
                     /locus_tag="ACG8FK_002784"
     CDS             2227..>2559
                     /locus_tag="ACG8FK_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5098320.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1016 a          307 c          457 g          779 t
ORIGIN      
        1 ttcttcatta atgttaatca agaagcctaa agaaatctta tcaaaaataa gttaggagaa
       61 aataatggat atattagaaa taaagaatgt agcatatagt tatgcaaatt ctaaagaaaa
      121 agttttgtca ggagtaaatc aaaaatttga acttgggaag ttttatgcga tagtaggaaa
      181 gtcaggaaca ggaaaatcta cacttctttc cttacttgct ggacttgata aacctcaaac
      241 aggaaaaata ttgtttaaga atgaagatat acaaaataaa ggatatagta atcatagaaa
      301 aaataatata tctttagtat ttcaaaacta taatttaata gattatttat caccaattga
      361 aaatattaga ttagtaaata aatcagcaga tgaaagtatc ttgtttgaac taggtttaga
      421 taaaaaacaa ataaaaagaa atgttatgaa attatctggt ggacagcaac aaagagtagc
      481 tattgctaga gcattggtat cagatgctcc aataatacta gctgatgagc ctactggtaa
      541 tctagacagt gttactgctg gagaaataat taatatatta aagacattag ctaaagatag
      601 aaataaatgt gtaatagttg taacacatag taaggaagta gcagattctg cggatatcat
      661 tttagaacta agtggtaaaa agttgaaaaa agtaaataaa atgaatttgg aggttgaata
      721 atgataaaaa atgcatttgc ttatgtaact agaaaaagtt taaaatcatt aattatcata
      781 ttagttattt tatctatgtc aactttaagt ctcattagtt tatctattaa agatgctacg
      841 gacagagctt caaaagaaac atttgctaat ataacaaata gtttttctat ggagataaat
      901 agacaagtaa atccaggaac acctagaggt gggggaaatg taaaaggtga agatattaaa
      961 aagatatctc aaacagatag tatagactcc tatgtaaaaa gaataaacag tgttgcagat
     1021 ttagttgatt atgatattat tgaaactcaa gagactcttg cgaatcaatc acctgaaaga
     1081 gcaaagaatt ttaaaagaac agttatgtta actggggtta acgactcaac aaaagaaaca
     1141 aaatttgtat cagaagcata taaattagta gaaggaaagc atttagaaaa tgaagataaa
     1201 aataaaatct taatgcataa agatttagct gaaaaaaata accttaaagt tggagataag
     1261 ataaaaataa aatctaattt atttgatgct gataatgaga aaggtgcaga tgaaacagta
     1321 gaagtagaaa ttaaaggtct attcgatggg cataatagcg gtggagtaag tgcagcacaa
     1381 gaactatacg aaaatacatt aatcactgat attcatacag ctgctaaagt ttatggtaat
     1441 accgaagata cagctgtata ccaagatgca acattctttg taaaaggtga taagaatctt
     1501 gatagtgtaa taaaagatct tgggaaatta gatataaatt ggagagaata taatttgatt
     1561 aaaagttcat caaattaccc tgcattacaa caatctatat caggtattta ctcaatttca
     1621 aataaattat tttttggctc attaatattt gcaggagttg tagtttcatt attattattc
     1681 ttatggatga atgctagaaa gaaagaaata gcagtattac tatcattagg tatatcaaaa
     1741 ttagaaattt ttggtcaatt cctaattgag atggtattta tatcaatccc cgcattcgtt
     1801 ggttcctatt tcttagctca atatacagct gacaagcttg ggaataatat attgaataag
     1861 gttactggtg atatagctaa acaaatagct aaacaatctg catctagcca attaggtggt
     1921 ggggcagaag ctgaaggatt taacaagaca ttatcaagtt tagatatcaa tgtattacct
     1981 aaattcatga tttatgtagt tatatttatg agtttagtac ttcttgtatc attgattatt
     2041 tcttcattca atatattaag aaggaatcca aaagaattat taattgacaa taattaaaat
     2101 tttcggtgct ttttagcacc aaaaatgaga gtgtaatata aaccgtgtaa gtaaccatca
     2161 atcctaggcg ggtggttgct tcacggtttt tatttttcta aaataagatc aagaggtgat
     2221 tacacgatgg ctagaaagaa aagaaatcct gatgctgaga agttagctga atccattttg
     2281 aatacttatc aacccgaatc tgtagaggat atgcaagatg ttttaaaaga tgtgtttggt
     2341 cccttatttg aaaaaatgct tcaaggagaa ttgaataacc atttagggta tgacgcccat
     2401 tctaaagagc caaagaaaca tgacaatcga cgaaacggtt atggcaataa gacgcttaaa
     2461 acgagctttg gtaaagtaaa cattgatgtt cccagagacc gggaggcttc ttttgaacct
     2521 gagttgattc ctaatagaaa acgagatgtc tccgacatc
//
LOCUS       DAZUMV010000205         2530 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000205 DAZUMV010000000
VERSION     DAZUMV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.214"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(195..560)
                     /locus_tag="ACG8FK_002785"
     CDS             complement(195..560)
                     /locus_tag="ACG8FK_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098321.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            768..1250
                     /locus_tag="ACG8FK_002786"
     CDS             768..1250
                     /locus_tag="ACG8FK_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098322.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1447..2337
                     /locus_tag="ACG8FK_002787"
     CDS             1447..2337
                     /locus_tag="ACG8FK_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFS5098323.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          876 a          410 c          499 g          745 t
ORIGIN      
        1 atgtttccac ctcaaattca atatctatta ttggaatcat tattgctgca attattacac
       61 aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa atagaaaaac tagtgatagt
      121 tttggtgata taagaaaaac atatatgtat aaaaagaaga aagtagcttc tctttgtaca
      181 tatatggttt gtttttatgg atgccgagct tttttataag ataaggctac cgcccataac
      241 gacccaagga tttgtggaac taagtaaata cctgcaagaa gcaagatcag ctgggtcggt
      301 tttaaaggat gatagaagta acgccattca gtaaaattgg cgcaaaataa ataggaagtg
      361 acagtagata taatattggc aatgaataaa gatataatac gcctgttcct ttgaaaataa
      421 tatccagcaa aaagagataa aaatatcaat gtgatgaacc cgcttacgga atgataacga
      481 aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag ataaaagcaa cggaaggttt
      541 ttccagcaag tttttatcat ctttcatcac cacctaacaa gtgttattat tttgttagtt
      601 ttattgtaaa ttgaatcctg caataatcaa ttactttttc attatttcta gattaaatga
      661 ataaactctc atgccattat ctctaaaaaa aaagtttttc cccctaattt ttttagatag
      721 aaaattgtat agtatgatga gagaaaaagt agtttggggg aacgttaatg agaagaaaag
      781 cagtaatcga tcggctttat ttattttttt ggttttttcc tatcgttttg ctacctttta
      841 catgggtaaa tgctgttgta caaggtgcgg agatattagg cctgatccaa ggaagcggat
      901 ttgtggtttc taggaatttt ccagctttga ttgttttcta tttagtgata agtgtaggtc
      961 tcttattacc aaaatctctg atcaatcact acaattggca gctgctttac ttttcttgct
     1021 tattagtatt tacatctatg ttttctttgc tgcctcgctt gattatcggc cctaatggtt
     1081 tgagcgagag tggattgaag gatttagctt attcctccat gtatttcttg acgatcaaac
     1141 catggttcta tttagtaatt gcctcattta ttcttgcaac atttttttac ttctggacag
     1201 aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa taactattga tggtggatca
     1261 tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc tcatatgctt tttttattat
     1321 tatgtaaaga atgcgaaaaa cgtttacatg atttttacat aacttaacta tatttttaca
     1381 gcaaccatac gagggattta cagtcgactg ataaacttta gttgtaaata agaaaggagc
     1441 aataaaatga aaaaagtatt gtttactcta ggtatgatgg gaattctttt agccggatgc
     1501 ggctcaggat caggaaatac agatggctct gccacacaaa ataattcaag tgactcaaat
     1561 gaacaagtaa aaatcgttgc ggtaggttcg acagcattac agccgttagt cgatgcagca
     1621 caagaaagct ttgtacaaga aaatccaaat tatcaaatct ctgtacaagg aggaggaagc
     1681 ggaactggtt tgagccaagt cgaagctgga gcagttacta tcggaaactc tgatgtattt
     1741 gcagaagaaa gagacggagt tgatgcttca aaattagtgg atcataaagt agcagtggtc
     1801 gggatggcac caatcgtcaa taaagataca gatgtcaaag atatcaccaa acaggaattg
     1861 attgatatct ttacaggaaa aatcactaac tggaaggaag ttggcgggaa agatcaaaaa
     1921 atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg cgacattcga aaaatgggga
     1981 ctggatggtg ctacccctgt gcagtcccaa gaacaagatt catcaggaac agttcggcag
     2041 ctggtaagtc aaacaccagg agcaatcagc tatttagctt tctcatatct cgatgattcc
     2101 acacaagcac taagtatcga cggtgtagaa ccaaaagaag agaatgtagc agataacagc
     2161 tggggaatct ggtcatatga acatatgtac acgaacggaa aaccttcccc tgaagtccaa
     2221 aaattcttag actacatgat gacagaagaa atccaagaag gacctgtcaa agaattgggc
     2281 tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg aaggaaatat caaataacta
     2341 gcagataaac atgccacggt agtcgttact attaaggaaa aatcaactat gtttccccac
     2401 atatacattt ttcctttaat tgtgaaaaat agttcctcat attttgccgt tttttgagct
     2461 aataccgagg gtcagctgat tgaacaccgt ttattcgaaa aaaagagcta cgacatagaa
     2521 ctgtcgtaac
//
LOCUS       DAZUMV010000206         2517 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000206 DAZUMV010000000
VERSION     DAZUMV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.215"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            296..910
                     /locus_tag="ACG8FK_002788"
     CDS             296..910
                     /locus_tag="ACG8FK_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5098324.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(934..1284)
                     /locus_tag="ACG8FK_002789"
     CDS             complement(934..1284)
                     /locus_tag="ACG8FK_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5098325.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1278..1541)
                     /locus_tag="ACG8FK_002790"
     CDS             complement(1278..1541)
                     /locus_tag="ACG8FK_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFS5098326.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            complement(1866..2045)
                     /locus_tag="ACG8FK_002791"
     CDS             complement(1866..2045)
                     /locus_tag="ACG8FK_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098327.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            complement(2061..2261)
                     /locus_tag="ACG8FK_002792"
     CDS             complement(2061..2261)
                     /locus_tag="ACG8FK_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098328.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
BASE COUNT          820 a          454 c          388 g          855 t
ORIGIN      
        1 caaaaataag aaaacgtttt cttaataaat cttaacagat aaatcaagtt atatactaga
       61 aaattcataa aatatatttt taatgtcata atcgtttaaa ttttcttgat tgacatttat
      121 ttacaaaaaa tattgccatt taaaaagaaa agtgacacat actatttcct ccatcattag
      181 tttttgtcta tataaggtat accctaacta gacacgaccg cgacattgaa aaatctggtt
      241 ttctcgtgta tgattagggt gttttttaaa tagttgcaga aagtaggtaa aaattttgag
      301 agttgcatac atacgtgtct catccatcga tcaaaatgaa caacggcaga ttgaagaaat
      361 gaaaaaattc ggtgcagaga gaattttcat tgagaaacaa tctggcgcaa cgattactca
      421 tcgacctgtt tttcaagaag cactggattt cgtccgagat caagatattt ttattgtgga
      481 agctattgat cgacttggaa gaaattacga cgagatcatc gcttcagtca attacttgaa
      541 gaagaaaaat gtgcagctga tcatcacaag tctaccgatc atggccgaag caattggcaa
      601 tcctctcctg gataaattca ttaaagattt gatcattcaa attttggcca tgatcgctga
      661 acaagaacgt accgaatcta aacgacgtca ggcacaaggc attaagattg caaaagcgaa
      721 cggtgtttat aaaggacgtc ctaagctata cagtgccaat gcaaaagatc cccaacgtcg
      781 tcttgtttat aaaaacatcg ttgaggatct aaaaaaaggt gttgcgatcg ctaagattgc
      841 aaaagactac aatgttacga gacaaaccgt atatagaata aaaaaagaca gcatggtcaa
      901 tcacgaatag ttgatcatgc tgctgtttct attttatttg cttacttttt gaaaaactgt
      961 tctaactttc atcaataact cctcaactaa actatcatgc gctttttcca aaaaaataca
     1021 ctctctggct tcataatcaa ttgtcgttag ttgatctaat aatacttttc ctgttgtctt
     1081 catttcatat tctgagttta tatttacata tagcggatag tttctagttg tactagaaat
     1141 tggagcaact gtaacaacgt tactataatc cgcaataata tcatgactta aaacaatata
     1201 aggtctttta cctgtctgtt cgtgtccaga tcgaggtgct gtattcaata atagaatatc
     1261 gccttgtcta ggttttctca ccatttttca ttacctactg cttcacctaa attttgaatc
     1321 gtcacatgat ttctttcatc agtgtaattt tcaaataact gttcaattgt taattcattt
     1381 tctatattag ccgcttcgat aattagccgc ttctgaccgt ttacttcttc ttgtattatc
     1441 ttcactttgt cttctgtatg aattccaaga tattccataa tatactttgg caatctaagg
     1501 ccattactat ttccccattt ttttataggt aacatttcca tagtagacac ctctttctgt
     1561 atatacatag tataacatac ataagaaaaa acgtatatac ttaattatcc ttttctatta
     1621 aattagatta acattccatg aatccataaa ctatgttaac atttatatga aaatagtcga
     1681 aaggaaatca taatatgttt aagaaaacaa aatctttaga atcaggtaag aaaaaaattg
     1741 atctggatac tttaaagatt tcatagttat tgatacaaaa tggaataaaa aggacataga
     1801 aaatattcta aatagtagga acaattaagt gctatcactt ttgtttcttc aaatgaaaag
     1861 tgacgtcact tttttagtgc tggtatatca aatcctgctt tttcttgcgc gatcaataga
     1921 tctaacgcaa tacacataat cttagattca ctttttccgt acttctgctt tagttgctgc
     1981 aattcttttt gtctcttttc atctaatgtc acgggtaacc gtttatttcc tattgcaatt
     2041 ggcattagta ctctctcctt tcatcgaaat aaccctatga aaaatatggc tgcaatcaac
     2101 ttgaatagta gactcacaag ggctaacaca aagcgccaaa aaatcaaaca aaatactagt
     2161 attcctaaga atactacgaa gtttaaatgt acagctgcac catgacgcat aagtatctcg
     2221 ccaatacaca gtacgaacaa cagaggaatg attttattca tatggatctc cttctttctc
     2281 ttaggttatc gggctttgcc cgcttatttc gcaccaccta agcggcgggg gcggttggag
     2341 cgattggtgc gaaataagaa ccattccctt aatttcaagg tttttgttct tatttcacac
     2401 attctcactt ttcggtataa atgattattg cctttttttc ctttcaaatc gttgatatgg
     2461 cattcgttta cggtcaagtt cattttggat ttcttctgta tcgtcgtaag tgtaggt
//
LOCUS       DAZUMV010000207         2491 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000207 DAZUMV010000000
VERSION     DAZUMV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.216"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(366..1658)
                     /locus_tag="ACG8FK_002793"
     CDS             complement(366..1658)
                     /locus_tag="ACG8FK_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5098329.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1932..2192)
                     /locus_tag="ACG8FK_002794"
     CDS             complement(1932..2192)
                     /locus_tag="ACG8FK_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098330.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          844 a          465 c          382 g          800 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt tcgaatggca
      661 ctaccataat caaatagttg ttcaacatgt gcaaagttcc gtttccagac atctacagca
      721 ccagaataat gagaccaccg attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga
      781 gaagaagcac cgtagaactt tttcatatct cggcagactt ccttatagtc cttacttgga
      841 atatagcgca atgcatttcg aacaagatga acaatacagc gctgaacaat taccgatgga
      901 aagatcgctt ttgcgccttc ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca
      961 tcttcgacac cacgtgcttt cagttcatca aatacttgca tccagcgatt tttagattct
     1021 gtttgattta accataatcc taaaatctcc ttatttcctt tgagatcata gccaagaatg
     1081 gtgtatacag catattcttt ggcttcataa ttttctcgta aagtaacata catacaatca
     1141 acgaatagaa aggcataaca ctttgctagg gggcgggctt gccattcttc caattcagga
     1201 aggacagcgt cagtgatatc tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct
     1261 tcgacggttg cggcaatatc tcgttgactc attcctcgtg catacatgga aagaaccttc
     1321 ccttcgatgt cggagacatc tcgttttctc ttaggaatta actctggttc aaaggaagct
     1381 tcccggtctc taggaacatc aatagctact tcaccaaaac tggttttaag cgttttagtt
     1441 ccatagccat ttcgacggtt atcgtgttcc ttaggctctt tagaatgggc atcataacct
     1501 aaatgattat tcaattctcc ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc
     1561 aaagcatctt gcatgtcatc gacagattca ggttgatagg cattcagaat ggattcagct
     1621 aacttttcgg catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1681 attgtagaaa aagaaaaacc gcgaagcaac cacctgccta ggattaatgg ttacttacac
     1741 ggtttatatt acactctcat cattaaaaaa gtatctttcg atattactaa acaatgaaat
     1801 tatctatttg aaatttaaat ctgataatat attgagtgtt ccaatgaggg ctttgatcct
     1861 attatccata tctttttcag ttttaatatt gatacacatt ataagaaaaa gaaacttttg
     1921 gttaatttat ttcatcccag tgcaaaatat aaaaaagtaa aatagaccat aggcaataga
     1981 ttaacaatta atataataca agcaaatatt ctattcattg tatttctgct ttcaaaaagt
     2041 tctaatattg aaagtataat tccacaaatg cctataataa gtatgggaat aagattagaa
     2101 aataacaccc cgagccaatc tgtgaaaatt actatattta ctaatatccc tattaacata
     2161 cctatcatat ttattaaatc aatttttttc attagatatt tctcctttat taaatctgaa
     2221 gtacttatat taaaataatt ctaaactatt tacctcaaaa agtgcaataa attatttctg
     2281 aacaataaaa tgatagtcaa aacaaaaaaa tattgttaat cgttcagagc catccgagag
     2341 tgtaaaatat tttgtgtaaa tgaaaaaatc catacaaaaa aggaaggcgc ttctgtagaa
     2401 taaagttaac gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca
     2461 gaaattgtgc aaactctagt cactaaaggc g
//
LOCUS       DAZUMV010000208         2487 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000208 DAZUMV010000000
VERSION     DAZUMV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.217"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..>2487
                     /gene="esp"
                     /locus_tag="ACG8FK_002795"
     CDS             40..>2487
                     /gene="esp"
                     /locus_tag="ACG8FK_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS5098331.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWP"
BASE COUNT          906 a          389 c          518 g          674 t
ORIGIN      
        1 ttactaggaa agtaattatt aagggagttg atttgaataa tggttagcaa gaataataag
       61 agagtatttc tagaaaaaac aaagaaacga gtattgaagt acagtatcaa aaaattaagt
      121 gttggggttg catctgtttt gattggtgtt ggtcttgtct tgggaacaac tgaactagtt
      181 aaagcacaag atgaaatttc tccaagcagc ccacttgaaa cagcgataag ctccgtacaa
      241 gtaggtgaca aagtagcttc ggggaatact tttcaggaga atcctggtta tacgaaaaat
      301 tataactttt cggatttaca atttagtcct caagaactaa ctggagacac actaaaaggc
      361 aatacaattg gatttgaagt ttacggaaaa cataatattg cagcatcaac taaaaactgg
      421 gaaatccgtc ttcaattaga tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat
      481 ccaaagaaag gaattggatc ttctagacgg acctttgtaa gaattaatga ttcgctgggt
      541 agacctacaa atatttggaa ggtcaattat attcgtgcaa gtgatggatt atttgctggg
      601 gcagaaacaa ccgatacgca aaccgctcct aatggggtaa ttacgtttga aaagagccta
      661 gatgaaattt ttaaagagat aggcatagac aatctcaaga ccgatcgttt aatgtatcga
      721 atctacttgg taagtcatca agacgatgat aaaattgtac cgggtattga tagtacgggc
      781 tactttttaa cagattcaga tgatttctat aatagcttag atgtatctga aaataatcct
      841 gaccagttta aacatggatc agtaagtgca aaatatgaga aacctaatac tcaaacaaaa
      901 gatggttcag gatccacagg tgctaatgga gctataatct tagatcataa gttaacgaaa
      961 aattataatt tctcatattc atcttccgca aagggcacgc cttggtatgc taattacaaa
     1021 attgatgaac gattagttcc atatgtagcg ggtatacaga tgcacatggt tcaggctgac
     1081 aaagtaacat atgatgtttc gtttgaatcc ggaacaaaag tagctgattt agcaatcgag
     1141 cgacgggaag gtcatgaaaa ttatggcata ggttcaataa ctaataatga tttaactaaa
     1201 cttatcgatt ttgctaatgc aagtccacgt ccagtcgtta ttcgatatgt tttacaattg
     1261 actaagccat tagatgaaat cttagaagat atgaaagcaa cagcacaagt tgaagaaaat
     1321 aaaccatttg gtgaagattt catctttgat tcttggttgt cggatacgaa taaaaaatta
     1381 atccagaaca cttatggaac aggttattat tatttgcaag atattgatgg tgatggaaac
     1441 cctgacgata aagaagagag cggagacacg aatccatata tcgggaaacc tgaattagaa
     1501 gaagtatatg atgttgacac aacagttaag gggaaagtat tcatccacga gttagcggga
     1561 acaggtcaca aagcccaact tgttgataaa gaaggtactg tattagcaga aaaaactatc
     1621 gctccaaatg aaaaagatgg ggctccaatt tcagatactg tagaatttga atttacgggt
     1681 gtagattcaa gtaaattaat cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt
     1741 tttgataaac cagaagaagg ttcaaccgtt attaaggaat caccaaaagc ggttgataaa
     1801 caaaccgtgg tagttggatt taaaccagat gctaaagaat caattcggaa taataaaaac
     1861 ttacctgaag atgcagagta ttcatggaaa acagagcctg atacttctaa cgttactgat
     1921 agtacgaaag gtattgtaac tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag
     1981 tttgctgtaa aagcttctca agctatggaa aatgatgcaa catacgtacc tataacaaca
     2041 actccagaaa cgacagttca aagtggaaaa cctacatttg ataaaccaga tgttccttta
     2101 gctaaagatg ccttttcaat ttcagatgtc tataataagg acttcggtaa tgcaagtgtt
     2161 gatgcaaaca ctggtattgt tacattcact ccagctaaag gtgtaggaga atcggagccg
     2221 attactggaa caattcctat taaaattgtt taccaagatg gttctgtagg cacgaccgat
     2281 ttagcagtaa ctgtaagtaa agatatttat gaaaatccag gagaaaacat tcctgcaggc
     2341 taccacaaag taaccttcac cgcaggggaa ggaacaagta ttgaaagtgg aacaacagtc
     2401 tttgcagtga aagacggcgt aagcttgcca gaagataaac ttccagtgtt gaaagcaaaa
     2461 gatggttata cagatgcgaa atggcca
//
LOCUS       DAZUMV010000209         2438 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000209 DAZUMV010000000
VERSION     DAZUMV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.218"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1420
                     /locus_tag="ACG8FK_002796"
     CDS             <1..1420
                     /locus_tag="ACG8FK_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS5098332.1"
                     /translation="KYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTI
                     DTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLT
                     DNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNR
                     SNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIV
                     VTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGT
                     TYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQI
                     VTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEP
                     TNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTIS
                     GGLILLGTLGLLGYKNRKKENE"
     gene            1554..>2438
                     /locus_tag="ACG8FK_002797"
     CDS             1554..>2438
                     /locus_tag="ACG8FK_002797"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5098333.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSS"
BASE COUNT          969 a          334 c          503 g          632 t
ORIGIN      
        1 taaatatgaa ccaacagtag aaggcgaaaa agtagaagtt gggggcacag tagatctaac
       61 agataatgtt actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc
      121 cggcggtaca attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta
      181 tccagacggt acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga
      241 cgccgataaa tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga
      301 tttaacagat aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt
      361 tactcccggc ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt
      421 aacgtatcca gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg
      481 ttctaatgca gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga
      541 gccagatgca tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc
      601 ttggaaagag aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac
      661 atatccagat ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc
      721 cgatgcagat aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc
      781 agatgcatct gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg
      841 gaaagaaaaa gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata
      901 tccagatgga tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc
      961 gaataaacct caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga
     1021 accaaatgca gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga
     1081 taaaaatggt aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt
     1141 aactgcaact gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa
     1201 caaaaaagac agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa
     1261 cagcaatcaa gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg
     1321 agagagtgac agtaatatct ttactattag cggtggttta atactgttag gaacgttagg
     1381 attattaggg tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct
     1441 tctctgagaa gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa
     1501 gtgttagtta aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa
     1561 aaatagtaat tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa
     1621 gcgattcaaa ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat
     1681 gtgggacatc gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa
     1741 atagaatgac actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa
     1801 ttgatattca aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag
     1861 aatatgacaa agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga
     1921 ataatttagc taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag
     1981 ctttggatga tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg
     2041 aaattgctga agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata
     2101 gagtgttgtc gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa
     2161 aaaatggaat acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga
     2221 ttgaaaaaat agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta
     2281 taggatttct atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag
     2341 catatctaac taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt
     2401 gtgctacaac tttttttacg tccattaata gaagtagt
//
LOCUS       DAZUMV010000210         2423 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000210 DAZUMV010000000
VERSION     DAZUMV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.219"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..184
                     /locus_tag="ACG8FK_002798"
     CDS             <1..184
                     /locus_tag="ACG8FK_002798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098334.1"
                     /translation="RKYRVSDFSTTSKNYCFFPLKCLQKVGSGRIYWQNQGEGSLNKE
                     KNHRKYKVITYKTSFL"
     gene            complement(471..974)
                     /locus_tag="ACG8FK_002799"
     CDS             complement(471..974)
                     /locus_tag="ACG8FK_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5098335.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1333..2358
                     /locus_tag="ACG8FK_002800"
     CDS             1333..2358
                     /locus_tag="ACG8FK_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5098336.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          769 a          381 c          459 g          814 t
ORIGIN      
        1 cagaaaatac cgtgtctcag atttctctac tacttctaaa aactactgtt tttttccttt
       61 gaaatgcctt caaaaagtag gatcaggcag aatatactgg cagaatcaag gagaagggag
      121 cctaaataag gagaaaaacc atagaaaata taaagtaatt acttataaaa caagcttttt
      181 atagaaaaat aatatacctg agtagggtat aaattaagac ggaattcctt tctagattga
      241 gatattgact aggaagaatg ttaagatttt aagcctttaa gctaaaaaaa aacccttttt
      301 gttaaataag gggaatatag cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat
      361 atagtagatt agagatgaaa atagattttt ttaaaccaat gactgaataa aaagaccgtc
      421 atagatacac attggaatgt gtctattacg gtcttttttt acgaacgatt ttattgaaga
      481 cggacagttc gggtcaaaaa taaaaaagct acgatcagaa ataagaataa aatgtatccc
      541 aaaataatgg ctagacggtc taacttattt gtcgatagat gtttgtttag aagttgatag
      601 gcccataaac ctatcagtcc tcctgttgta ttagttatca aatcagtgat gtccgctacg
      661 ccaataccga ataggaattg aatcaattct gccaagaagc ttacaaggaa aataatccct
      721 aattttttag aaaaagaaag tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa
      781 aagacaatca agttataagt ggtctcacgt aataccgtcg tattttcaaa agaaaaagta
      841 gaaaaaggga cgagattgac attgctgtaa gaataagtga gaaattgtgg aagattaaat
      901 gatagcttga ataaaatcaa ccataataga aagaataaat aaatccctaa taaaaatttt
      961 gatacatacc gcatagacat ctcctccatt taaaaagtat accatcgaaa accgctgaaa
     1021 aatcataaaa aagtatgaag tttaaggacc aacgtagtta tcgtacgaaa attagaagct
     1081 tttttttgtt gggaatccgg tttctttaac aaagctaaga tagcgagttg cttggaaaag
     1141 ttgctatctt ttttctgtca aaacgactcg gaatgttcgt tttttgttct aatttctcca
     1201 agttgtttac tttttgatac taagtttatg aaaaatcaag aaatagttgt tatactaggt
     1261 aatgctgaca aacgggattg gcatttcatc ttggttcagc tttttttcca attactagga
     1321 aggagggata gattgttcaa gacttatttt cgtttcaata aaatacaaga taatcctttt
     1381 cgtatcatga atctgatgct acttatcttt attgtattat ggttaggtta cataaatcat
     1441 gattttttga tttcaagttt ttcatcatta gggatcttta cttttttcta ttatcaaaac
     1501 ataccgatga aatatttgct gaaacgtatg tttctgatcg gttgcggact gttggttgct
     1561 ttcgttttgg ggatcttatc gacttatgtt ttatggcttg aaccatttgc cgtagctttg
     1621 gttgcttttt ccagcagatt cgtgttgcga ttattccata tttctaaacc aggtggcttg
     1681 ttttttgcga tgctttctgc catgggaacg agcatgcagc tgccaatcgc tcaattaccg
     1741 atcgtctcac tttatttttt tatgggtgtc gtttttgccc tgattgcggc agtgattaca
     1801 aaattactgg attcacggcc tgagcagaca attgaaaaag caactttaaa agaaagattt
     1861 cacgaagagc ctttagtgat cattgattct gttttctaca gcgctgcctt atttctttcc
     1921 gtttatgtca gtcacggctt gaatctgcat aatccttatt ggttgacctt gtcatgtgca
     1981 tcgattttgc tagcagaaaa cttggatgcg atgaagcatc gacaagtcca atatctgatt
     2041 ggatcgatgg gtgggttatg tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg
     2101 cagaccattt ttctgatcac ttttctatat ggaatcgcac aatttctagt tgctagaaat
     2161 tatgcagttg ctaatatttt tcttaatccg atggcgttga tgctatctac acttattcgt
     2221 ggagcatact tgatttcttt gatcgaatat cgttttttag ggatcgtcat aggaagtttc
     2281 atcggtttag gtgttgcgtg ggtaatgacg gttggtttgc aacattattt aacggttgtt
     2341 cgtcaaaaat tagaatagaa ggacaaatat atgtgccaaa agaggttatg acaagatttt
     2401 tgtcacaacc tctaattcaa ggt
//
LOCUS       DAZUMV010000211         2401 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000211 DAZUMV010000000
VERSION     DAZUMV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.220"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8FK_002801"
     CDS             207..2069
                     /locus_tag="ACG8FK_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5098337.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..2401
                     /locus_tag="ACG8FK_002802"
     CDS             2069..2401
                     /locus_tag="ACG8FK_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5098338.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
     2401 a
//
LOCUS       DAZUMV010000212         2365 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000212 DAZUMV010000000
VERSION     DAZUMV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.221"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1815
                     /locus_tag="ACG8FK_002803"
     CDS             202..1815
                     /locus_tag="ACG8FK_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5098339.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          745 a          418 c          453 g          749 t
ORIGIN      
        1 ttcagctttt aaaaaatata cagggttatc cccttcaaag tattatctat ccttaaaata
       61 gaaaaaaatt tttaggtttt atccacaaat tatttctcta tattttctgt ctattcaaaa
      121 taaacaataa ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa
      181 cgtagaaaag gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct
      241 tcatttgcca ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc
      301 ggagaaaata tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact
      361 gaccgtgact cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat
      421 gtctttacta aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa
      481 gacctatcca caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt
      541 gctagaccag attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc
      601 cgtagtgtga cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat
      661 gtcgtcattc ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc
      721 ggcttagacg tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa
      781 attgctaatt tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca
      841 agagttactg agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt
      901 tctgctgata tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca
      961 accggcatac aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat
     1021 ctgccggaca tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag
     1081 ccgatccaaa gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt
     1141 ttaaatggta cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt
     1201 gatcatacag caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca
     1261 aattataaaa ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca
     1321 agattaaatc taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca
     1381 tttggagaaa aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac
     1441 ttgacagtct ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc
     1501 cgtgacccgt tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa
     1561 acgatcaaag caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata
     1621 cgtgaaccta ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat
     1681 gagttacgag atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga
     1741 agaggattga atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt
     1801 ttgattgaag aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac
     1861 ttgaggagta aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa
     1921 gataaatgtg aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa
     1981 gtttaaacat tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt
     2041 gactctgaga caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac
     2101 gaggcatcat attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt
     2161 ttcttatata ataaagtttg ctttcactcc tctaatctca ccaaatcgac tggcttgcgt
     2221 tgtgtgatac taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg
     2281 ccatcctcgt tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt
     2341 taattttttt gttcctattt attta
//
LOCUS       DAZUMV010000213         2360 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000213 DAZUMV010000000
VERSION     DAZUMV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.222"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            466..1425
                     /locus_tag="ACG8FK_002804"
     CDS             466..1425
                     /locus_tag="ACG8FK_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5098340.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1412..2017)
                     /locus_tag="ACG8FK_002805"
     CDS             complement(1412..2017)
                     /locus_tag="ACG8FK_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098341.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2257..>2360)
                     /locus_tag="ACG8FK_002806"
     CDS             complement(2257..>2360)
                     /locus_tag="ACG8FK_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFS5098342.1"
                     /translation="WTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          775 a          401 c          371 g          813 t
ORIGIN      
        1 gtcattaact tatgatagta gtcaatttgc ttttcactaa ttacaaaata tgtgatatag
       61 gaagtttagt tattgttctc ataatgccat cctgttttta gattatctag taattttttt
      121 gttatttaaa attaggagca gtgccaattg ttttaatttt aaaggctttt tttatatctt
      181 ctaaattaga agatatttgt atatcaatat ggaatatatt accttttttg tattttttta
      241 tgttatcttt agctaaaaca tcaaatacat gtccttgaaa ttgtatcgag gcagctggat
      301 ttagtttaaa ttgaggaaac tttaccacag ctgattcaaa acacttgtca cattttttta
      361 tttttttacc ttcgccgatt gtaaaattaa gctagacaac taaaaaagtc atttgtgcta
      421 cactcaaaat agttccgtcg aaagaattat aaggagtgag tacaaatgac ctatacccat
      481 cttacaccaa ctgaactcgt aatgatagaa gcttatttca atcaatcaca ctctgtttca
      541 aaagtagccc atctggtaca acgttctcga caaaccattt ataatgtcta tcgttttctt
      601 aaatctggtg gaagcgcaat aagctactat gaacaatata agaaaaacaa acgtaactgc
      661 ggaagacgtc cgattgtgtt accagatggg caacaagaat acattcaaaa aaaggtggct
      721 caagggtgga ctccagatgt ccttattggt cgagcagagt ttcctattgc ttgttcggta
      781 cgtacactat atcggatgtt taaagataaa acctttgacc ctcacgattt accaatgaaa
      841 gggaaaagaa aaccaaacgg acataaagaa aaaagaggaa aacaagcttt tcgccgttcg
      901 attcaggata gaacaactga ttatatacag ttcaatcatg agtttggtca cctagaaggt
      961 gacaccattg tcggtggaaa acataaaagt gcggtgatca cgttagttga gcgattatct
     1021 aaagtgatca tcaccttgaa acccgctggt aggcaagcta tagatattga gaatagactg
     1081 aatcaatggt ttcaatctgt accgaaacac ttgtttaaat ccatcacatt tgattgtgga
     1141 aaagagttct cgaattggaa aaagattagt aataccaatg atatttcgat ttatttcgct
     1201 gatccaggaa caccttctca acgaggacta aatgaacatt cgaatggctt attacgtaaa
     1261 gatggcttat ataaagctat ggattttaat aaggtatccg aaacctttat ccaatcggtg
     1321 gcatcaaaac gaaatcatat tcctagaaaa tcattacact atcgcacacc attggaagta
     1381 tttttgagtt acgtaggtaa agatgatttg tctagcttaa tttgacaaac gaaactttgt
     1441 aaaaatattc gattgaatat tctatttggc acgactcact tggcagtata tgtatgtcaa
     1501 tatttttcat ctgtttcata ggtatttcaa aatctaaact attaaaaaca tataattccc
     1561 tgtaattatt tacatctaac tctactaaat ttatctttgg aacaatagat cctccaccca
     1621 ttccaccata ataaaaaaaa tcttgttctc tatcaaaata gcacatatta agctcagttt
     1681 gtttatcatc ctcaataatt ttataaatca aacctatata atcatgaatg ctggcaaaaa
     1741 ttttttttaa gtcaagttct aaatcttctt cgaaaacaca tttaatttct cctactgata
     1801 attgatactc tatattacat gacttaatta gttttttaga tcccccctcc agataggcat
     1861 atgtttcaat aattatgttc tttttattgc ttggcaaatt tccgttattt attgcataaa
     1921 ttaaaaatga ctgtctattt agcccataat ctaatacaac ttcttcatac tcatatttct
     1981 ttaaatattt aatatgaaaa tatgctgaat taatcaaaac tcgagtgtta ctgttgttat
     2041 ttattgttgt tctattgtgt ataaaattgt ataggttgtc atattttctt ggtttagtga
     2101 actactcacc acttagctaa acctaacggt ttcttacgtt tgaagtggga gcttcttggg
     2161 aagagagcat acttgatagc gtattacttt actaacagag gtgcctctta tttaccaagc
     2221 tatccccgta gttcctacgg ttcttgttta tttgatctaa gccaatccca tagagtgtag
     2281 ccttagacct tcggtcagga tattgatact agcgttgata tctcggtcat gatgagtatg
     2341 acaaataggg caagtccatt
//
LOCUS       DAZUMV010000214         2347 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000214 DAZUMV010000000
VERSION     DAZUMV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.223"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8FK_002807"
     CDS             complement(<1..300)
                     /locus_tag="ACG8FK_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS5098343.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8FK_002808"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8FK_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8FK_002809"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8FK_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098344.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="ACG8FK_002810"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="ACG8FK_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          676 a          442 c          334 g          895 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaa
//
LOCUS       DAZUMV010000215         2345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000215 DAZUMV010000000
VERSION     DAZUMV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.224"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..2311
                     /gene="acm"
                     /locus_tag="ACG8FK_002811"
     CDS             146..2311
                     /gene="acm"
                     /locus_tag="ACG8FK_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS5098345.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          921 a          351 c          454 g          619 t
ORIGIN      
        1 atttattttt tataaatatt tgtaaaataa ctatcaattt tttagttaaa agattggaaa
       61 tgagtggggg aggaattgaa gaaaggattt ttgagagatg atatagtagg ttttattttt
      121 agttaaaata ggggagatag aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt
      181 atttattatt actaatataa catcactgat tcctgttcat gtctatgcgg atgcaggcag
      241 agatatcagc agtaatgtca cttcgttgac cgtggaccca acgaatatta cagatggagg
      301 aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa aatatccaac caggtgatta
      361 tctttggatc aattggccaa gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat
      421 cccattaatg attgaaaata aaaatgttgg aactttaacg gtcagaaaag attctgcgca
      481 ggttgtgttt aatgaaaata ttaaaaacct agactcagta gaaggttggg ggcaatttga
      541 gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat acgggaccat tcaccgtgac
      601 gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca gcttctggtt cttcttctag
      661 cgttttttat tataaaacag gagacatgct acctgaagat actaagcaca ttcgatggtt
      721 tttgaatatt aacaataacg gcacttatgt tgaacaacca gttaaaatca gtgatgaaat
      781 ccaaagtgga caaagattag atccaagtac gtttgaaatt aatcaaatac acttaggtga
      841 acagaaggtt tatagaggag aagagggaat ccaacaattt ttacaagatt tccccagcgc
      901 tacatttaat ttttcagtaa ctgataatta cattgaaata actataccaa aaaactttgt
      961 caatcttagg aaaataatgg tgagttataa gacgatcata gaaaacccag aacaaataaa
     1021 ttttgagaat cattcggaag cttggtttaa agaatttaat aaacctgctg ttgatggaga
     1081 aagtttcaat catacagtta aaaatatttc agcttctggt ggagtcaacg gtacagttag
     1141 aggcgaattg aagattttca aatatatcaa tgacacagag attggtatcc caaatgtgac
     1201 tttcgaatta agacgagcag atgaacagcc tattcaagga cagtctagta tcctgttgac
     1261 ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa
     1321 agaaaaagaa gcacccaatt ggattgattt tgatccacta agttcaaatg aactcaaatt
     1381 ctccataaac gaaaatgata cagagggagt cagtttacct atttataaca agaaaaaggt
     1441 tacgaacatc acagcaacta aaatttggaa cggtggaacc actccaagac cgagtattta
     1501 ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa
     1561 acgattagac aatggaataa cttcagtgac ttgggaagat atccaacagt atgatgatag
     1621 tggaaacgaa tatacgttta aagtgcaaga agtagatcaa aacggaaatg attatgtccc
     1681 ttcaggttat caaaaaattg aaaacgggct agtcgttaca aatgagaata aagaagtcat
     1741 ttcagtctca ggacaaaaaa catgggaaga caatgagaac caagacggca aacgaccgac
     1801 gacgatcaca gtaaatctat tagccgacgg tcaaccgatc caacacaaag aagtaagtga
     1861 aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat
     1921 catctatact gtaacggaag acaacgtgcc cgaatacagt acgacgattg acgggtataa
     1981 catcaaaaac agttatacac cggaaaaaac aaatatatct ataccaggta gccatataac
     2041 accgtggaaa aattttccag agaagacaga gagagagaaa attagaaatc tattctctca
     2101 aaaattgcaa atatttgctc atacaggaga atccagcgaa caaagaagca attatattag
     2161 taagagagct gtacctaaac agataacaaa taaatcaaaa agcattcttc ctaaaacaag
     2221 tagtaaaaaa tctagttttg ctgtaatcat tggtttattg atagtaacta tctgtgctgg
     2281 gatattctta caaaaatata aaaaagttta gttagaatat aatatttttt gttaaatatg
     2341 actga
//
LOCUS       DAZUMV010000216         2307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000216 DAZUMV010000000
VERSION     DAZUMV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.225"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..948
                     /locus_tag="ACG8FK_002812"
     CDS             <1..948
                     /locus_tag="ACG8FK_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS5098346.1"
                     /translation="STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQ
                     LWKPAFIILTPQLFMQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIV
                     MLPKISKAQAEGKSENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPM
                     TINMLISCLIIVVSPIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGA
                     VAATIVLVVVELLVFFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLF
                     SSSFLDMAIKSIMIFIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(1015..1167)
                     /locus_tag="ACG8FK_002813"
     CDS             complement(1015..1167)
                     /locus_tag="ACG8FK_002813"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5098347.1"
                     /translation="MLITKLENLRTHIKTLKRFAYGFRSFTNMRLWIFLINRLISYAL
                     KASNSY"
     gene            complement(1270..2070)
                     /locus_tag="ACG8FK_002814"
     CDS             complement(1270..2070)
                     /locus_tag="ACG8FK_002814"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5098348.1"
                     /translation="MRLRKQHYHYQSCGKYFTAHTYLVVPSCFISKQVHYTILEELTE
                     RQALKTLTKRCDLSVTTFQRTLSALSAMLRQMLDYLPHCLLFDEFRSLSISHGKFSFS
                     CMNRETGKLFDILSSRRKKDLIAYFMRFTRKAHMGVRYIVTNMNAPYFSLVKESFPNA
                     QIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKDTSQAKYYRQLKPLYKLLLKSDDELDY
                     TTFKKWQNFQWRYLTESEVVDRLPSISYELKIAKQFIKSCLAIITRNS"
     gene            complement(2077..>2307)
                     /locus_tag="ACG8FK_002815"
     CDS             complement(2077..>2307)
                     /locus_tag="ACG8FK_002815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5098349.1"
                     /translation="CISISRSIKEHFTIKDKNIEINSFQKTFYKDKKAIVIEGTLVRT
                     FRHCLFCKETAKQLVKNGKKISMIILNRSGNK"
BASE COUNT          722 a          347 c          413 g          825 t
ORIGIN      
        1 tcaactaaag atgatttgtg gatttacgtg ttcattactt caattactaa ctttttagga
       61 agtttagttt tctggttaag tatccacgga aaagtaggta aaccaatctt taactctaat
      121 gtttttaaac agttgtggaa accagctttt attattttga ctcctcagtt gtttatgcaa
      181 gtgtacacaa ctttagacaa aacgatagtt gggtattttg tggatccaac ggaattatct
      241 tattatgatc agtctcaaaa aattgctcgt attatattgg cagtcttaac atcaattact
      301 attgttatgt tgcctaaaat atctaaagca caggcagagg ggaaatcaga aaatatttta
      361 gtttacacaa aaaaatcttt tgattacacg attatcattg cattaattct ttttagtgtg
      421 gtgtttatca atacaaaaga atttgttcca tggttttttg gggagaattt caaaccgatg
      481 acaataaaca tgcttatttc ttgtctaatt atagttgtta gtccaattgg tggagttttt
      541 gctaaccaat ttgcaattgc tatagaaaaa gataaggagt atgcttatcc aatgatcatt
      601 ggtgcgattg taagtattat tggaaatgta gcttttgtgc ctacttatgg cgctgtagcg
      661 gctacgatcg tactcgtggt agtagaatta ttagtctttt tctttagagt attttttgta
      721 cgaaaagaaa taaaaatatc ctatttcttt gggaagaacg ttatcttatt ttttatagtc
      781 aatcttactt tgactacttg ggcctattac tttcttccac aattattctc ttcttcattt
      841 ttagatatgg caattaaatc aataatgatt ttcattattt atcttccgtt tgtttatttt
      901 ctttttccag atatacgaaa tgaaataaga aagattactc gaaaataaaa taatgttgta
      961 aaaaagtagt tctttgtcaa atcgggctga taaggtcatt tatgaacaac aaagttagta
     1021 agagttacta gcttttaatg catagctgat taacctgttg attagaaaaa tccacaagcg
     1081 catattggta aaactgcgaa acccataagc aaatcgtttc aatgttttga tatgtgtgcg
     1141 aagattctct aattttgtta ttagaatact ttttcgaaaa tgctagttaa aagccttttt
     1201 cagatatgaa aaaggctttt tgagatagtg aagtttcgag ggaacttctt tggtcgtttc
     1261 tcgaagtagc tagctgtttc ttgtgataat tgctaagcag cttttgataa actgcttagc
     1321 aattttaagt tcataagaga ttgagggtag acgatcaact acttcacttt ctgttaaata
     1381 gcgccattga aaattctgcc atttcttgaa tgttgtgtaa tccaactcgt catctgattt
     1441 tagaagtaat ttatacaaag gtttcaattg tcgatagtac ttagcttgtg aagtgtcttt
     1501 ttgattcaac tgcttcatca cacgttttcg aacacgatcg aaaaacttgt tcaagtgttg
     1561 tccaatgtga aagcaatcaa tgatgatttg ggcgtttgga aaggactctt tgactaatga
     1621 aaaataaggg gcattcatat ttgtgacaat gtatcgaacc cccatatgag cttttcttgt
     1681 aaaacgcatg aaataagcaa tcaaatcttt ttttctccgg ctggacaaaa tgtcaaagag
     1741 cttccctgtc tctctattca tacaagaaaa ggagaacttt ccatgggaaa ttgaaagaga
     1801 gcgaaactcg tcgaatagta aacaatgagg taaatagtct agcatctgtc gcagcattgc
     1861 cgatagagca gataatgtcc gttgaaatgt cgttactgaa agatcacaac gtttcgtaag
     1921 tgtctttaac gcttgacgct cggtcaactc ttctaatatc gtgtaatgaa cttgtttcga
     1981 aataaaacat gaaggaacga caagatacgt atgagcagta aaatattttc cgcatgattg
     2041 gtagtggtaa tgttgttttc taagacgcaa ataggtttat ttattcccac tgcgatttaa
     2101 gataatcatt gaaatcttct tgccgttttt cacgagctgc ttcgcagttt ctttacaaaa
     2161 tagacaatgt ctaaaagtgc gaactaatgt accttcgatt acgatggctt tcttgtcctt
     2221 ataaaaagtt ttttgaaaag aattaatttc aatattttta tctttaatag tgaagtgctc
     2281 tttgatagaa cgagaaatgg atataca
//
LOCUS       DAZUMV010000217         2291 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000217 DAZUMV010000000
VERSION     DAZUMV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.226"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..2156
                     /locus_tag="ACG8FK_002816"
     CDS             3..2156
                     /locus_tag="ACG8FK_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS5098350.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
BASE COUNT          784 a          412 c          508 g          587 t
ORIGIN      
        1 aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa
       61 aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa
      121 attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt
      181 tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg
      241 tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga
      301 ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc
      361 ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc ggtagatgaa aatctgacag
      421 atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa
      481 gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata
      541 aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta
      601 aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg
      661 atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca
      721 cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga
      781 cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt
      841 ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag
      901 gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag
      961 aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat
     1021 caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac
     1081 agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa
     1141 tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga
     1201 ttactagcgc ctttgctcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg
     1261 caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg
     1321 cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag
     1381 cattggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag
     1441 actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat
     1501 tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg
     1561 aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca
     1621 aagaatggta tactccaggt aactttaccg atttggcatt tggacagttt gacacatata
     1681 caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc
     1741 cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga
     1801 aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc
     1861 tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacagca gccaaaccat
     1921 tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag
     1981 gtcatccaga tattacgaca gtcaatagta atatcgtggc atacggacca acagacaatc
     2041 cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac cacatgaact
     2101 tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa
     2161 gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc
     2221 tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact
     2281 cggcaaaaaa g
//
LOCUS       DAZUMV010000218         2188 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000218 DAZUMV010000000
VERSION     DAZUMV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.227"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..814
                     /locus_tag="ACG8FK_002817"
     CDS             140..814
                     /locus_tag="ACG8FK_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098351.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
     gene            complement(975..2147)
                     /locus_tag="ACG8FK_002818"
     CDS             complement(975..2147)
                     /locus_tag="ACG8FK_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5098352.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT          700 a          436 c          394 g          658 t
ORIGIN      
        1 aaaaatcccg tgtatcaaaa cgggattaga ttaagtttcc tcaatcacta ttcataacac
       61 aattattata acaagtaagt aaacaattga aaaggacatt tattgaaaac ggttgttttt
      121 tacttaagga ggaaatttag tgaaaaaatt agttgcattg tttttggtcg caggaagttt
      181 gttgagtggg tgtggtgcta ctacgtctac tactcaaagc acaagttcag ctcaggcttc
      241 cagcagcagt accacgatct ccgaaagtac ggcagattca tcgaaagaac aagaacaaat
      301 tgaaaagaaa aaagaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaaagt
      361 gacagaagcg gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag aaaactatgc
      421 aactgcttca gcagctatcc aagcgctacc tgaaaacaaa caagagttga caagccgtct
      481 tgctgcaatt gattccacaa tcaaagcaaa agaagcagaa gaagaacgga aaaagcaaga
      541 agaaattgca cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac agcaacaagc
      601 agaacaggcg cgacagcaag cagcagctga acaggcctct cagcaggcac aagccgcagc
      661 tcctgagcaa aataatgaac aaactgtcta tgtcacaccg accggttcaa aataccatac
      721 acataaatgt ggaaatggta cgtatacccc tgccacttta agcgaagctc aagctcgtgg
      781 tttaacccct tgctccaaat gttatggaaa ctaattcaaa agttatacta taataataaa
      841 attcttctga aacataaaaa atagaagctg gcgttccaga tagtaaacca aaaatagcag
      901 tttacacgtc catttcgtca gcttctctct tttttcattt agtctgttcc ctctgctttt
      961 ttggacatct tcattcattc tttctcaatc aaatagtaca cctctttttc ttttcgcagt
     1021 ttatatcttc cttttttcaa tttcattgaa aactgtttcg aatgtttatg ttttacatgg
     1081 attttaagca ggagctcctc atgttgcaag cgatagttcc tgccttttaa atcagaaaat
     1141 ctcactagtc cgtattcatc agaagctttc acaaatggaa ctttttctct agtaatatag
     1201 tgtttgccgt tgagcgtttt tacagaaaag accattcctt cgattggtcg tccgtttgtg
     1261 ttctttacat aaaaagataa aggatagttt cgcttagcga tggccaaaga atctaaataa
     1321 gcgatgagta gccaaactaa gccgattgat ccaagtagta taccgataaa ccaaataaaa
     1381 aatttccatt ttttccattg atcaattcct tggatcgctt tttttgcctc ttttgcttga
     1441 taaggaatac gatgccccgt cacaagcagt cgatgtgtat tgaccatata aggagtacag
     1501 gtaagcaaag tcacgagatc tctgcctttt tggataccca aagaatctac ttcatcaggt
     1561 aagaccactt ggattttttc tacttgatac gccaatgtct ttccattgat ctggatgaaa
     1621 aactggtctc cttttttcaa cttaggcaaa tctgtaaata attttgcttc tgggagtcca
     1681 cgatgcccag aaagaacggc atgggtgttt ttcccgccag tcggataaga actttcttcc
     1741 aataatgacg ttcctttttg caaaaatatt tccgttgtct gatcgaatat aggtaagctg
     1801 acattgattt ttgggatttc gatgactcca agcatatgtt ccataaaaaa ggcattacta
     1861 ggtaaatctt ttaatgcttt tgcatcaact gcttggttga aactgcctat ccctggtgat
     1921 acgttttgct ctgccaactg ttggtttttc tttgtcattt tttcctgctg tttttttatt
     1981 tcctctgaat tcttattcga tgcttgcttt tgataatgga tgatcatttg ctgggcaaga
     2041 aaatcattca gtgaatcttc tacaaaagga taagaaaaga tcccgatccc aacgactaaa
     2101 atgattgcca tgaagatgtc cagtattctt attctgcttt tattcatcat gtccacctat
     2161 tttctaatat gtatatgaag aggttgtg
//
LOCUS       DAZUMV010000219         2171 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000219 DAZUMV010000000
VERSION     DAZUMV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.228"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..903
                     /locus_tag="ACG8FK_002819"
     CDS             289..903
                     /locus_tag="ACG8FK_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5098353.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1068..1892)
                     /locus_tag="ACG8FK_002820"
     CDS             complement(1068..1892)
                     /locus_tag="ACG8FK_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098354.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          733 a          365 c          312 g          761 t
ORIGIN      
        1 aatctaataa aaactctcac tctaaaagag tgagagactg caaattagag tacggacttt
       61 tttggagttc cgttctttaa tattaacaaa tgaataaaat ccttacaaca tttgagaata
      121 agttatcagt agacacttac aaaaaattct catactattt cctccatcat tagtttttgt
      181 ctatataagg tttaccctaa atagacactg gtataacatt gaaaaatatg gcttcctcgt
      241 gtatgattag ggtgtatttt aaatagttgc agaaagtagg taaaagtttt gagagttgca
      301 tacatacgtg tctcatcgat cgatcaaaat gaacaacgcc agattgaaga aatgaaaaaa
      361 ttcggtgcag agagaatttt catcgagaaa caatctggcg caacgattac tcatcgacct
      421 gttttccaag aagcattaga ttacgtccga gatcaagata tttttattgt ggaagctatt
      481 gatcgacttg gaagaaatta tgacgagatc atcgcttcag tcaattactt gaagaagaaa
      541 aatgtgaagc tgatcattac tagtttaccg atcatggctg aagcaattgg caatcctctc
      601 ctggataaat tcattaagga tctgatcatc caaattttgg ccatgatcgc tgaacaagaa
      661 cggactgaat cgaaacgtcg ccaggcacaa ggaattaaga tcgcaaaagc aaacggcgtc
      721 tacaaagggc ggcccaaatt atacagtgca gacgcaaaag atccccaacg tcgccttgtt
      781 tacaaaagca tcgttgaaga tcttaaaaat ggtgttgcga ttgctaagat tgctaaagac
      841 tacaatgtta cgagacaaac cgtgtataga ataaaaaaag acagcatggt caatgacaaa
      901 tagttgatca tgctgctttt attatcttct catctttaat ttcctacata taattactat
      961 ctataaaatt aagtcttact cagaacagat aaagcataaa attccaacaa accatttttt
     1021 acttaactaa ataaagagtc tgttaatata atcttcactt atttacgtta atcccctact
     1081 gtatttgttg gttccttatc tatattttct acaaaatcct tattatcttg tgatttagct
     1141 tcgtataatt ctttgtatgt cgacacattg gttatactaa ttcctatagc tagttcttga
     1201 gactctactt ttctctctag ctcctttgac aaagtgattt gagatttatt tggttccgtt
     1261 ggagtcaatt ctatagattc ttcataaaaa tctctataca taaagctatc attaatttta
     1321 taaaaaaaat taaatgtttt tagcactgcc atataacatt caaaacattc ttctatagtt
     1381 ggtttacttc cttgaggaga atgaaccaat ttattacgtt tttctatgaa atttttaatt
     1441 tcctttctta tttctttcgg aaactcaata ttaaagtacc ctttaaacat gctccctaat
     1501 aatttgtcta cagggggaga ttgtaaattc tcaaatatta gagttaaatc aggtttctct
     1561 tgttgataaa attcctttac ccctatttca caggctgtaa ttatcgaaat aaacctatct
     1621 ctagtatttg atatattttt acttctttta aggagtataa gagaaaaggg gatactttcg
     1681 tgtccatgca tgtaattaac taattgttga gcttcagaag ttatatctct aattatatag
     1741 ttaacggaat taggaagtgt ctttaactct aatgaaattt ctatccaatt actatcttct
     1801 aatctgtata tgctcgaagt ataatttgat tcgtcagcaa gcatcgctac atcccaaaca
     1861 ctgatcataa aatcttttaa acgttcaatc atttcagcaa tattgctaaa atcagctaat
     1921 ttttctttag aataaaaaaa aacatttata tcagaatcaa taaattctat ttttttatta
     1981 ctatatcgtc atcattatct tgaccattta tgtttattgt tccctgagtg gggagtactt
     2041 ctttcaaatt caagttaaat ttatattcca ttacttctcc tccaatatat aaaaattact
     2101 taactgtatc caaatttaat atgtaaataa atcattaatt tttagctaaa ttctatgaga
     2161 agtttatttt t
//
LOCUS       DAZUMV010000220         2138 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000220 DAZUMV010000000
VERSION     DAZUMV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.229"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..565
                     /locus_tag="ACG8FK_002821"
     CDS             <1..565
                     /locus_tag="ACG8FK_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098355.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
     gene            complement(612..848)
                     /locus_tag="ACG8FK_002822"
     CDS             complement(612..848)
                     /locus_tag="ACG8FK_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098356.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            946..1125
                     /locus_tag="ACG8FK_002823"
     CDS             946..1125
                     /locus_tag="ACG8FK_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098357.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1256..1525
                     /locus_tag="ACG8FK_002824"
     CDS             1256..1525
                     /locus_tag="ACG8FK_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5098358.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1518..1775
                     /locus_tag="ACG8FK_002825"
     CDS             1518..1775
                     /locus_tag="ACG8FK_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5098359.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          786 a          299 c          428 g          625 t
ORIGIN      
        1 agaacaattg tatttggaaa gtgaaacgag tcaaacgaag gtcacagtca gccataatcg
       61 tttagacgtg tataaacgtc gtcctgaaac ggtaaaaaaa ggaacggagc ttctacgact
      121 agaaacagaa aaatacatca tgaatgttta cctagaaagg agtgagctgg tcgaaaactt
      181 tgtcagctac acgatttgca ggaaacacta ttactcagaa tcagaatatt attcaatttt
      241 tgagagcatg gaattaatgg aagaagaaat ggaagagcta aacagcaaag atgcgacgat
      301 agacgattat taccttgtat ttactagatt tttgacgtgt ggaacggaat ctatcgtgtt
      361 aatgggaatg gattatgaac tcgctgtacc cgaagcattt ttgagggaat atgtagaaga
      421 agtagataac ccaggcgacg actggtacac gactttaact gattttttag acacctatac
      481 ttacgacgat acagaagaaa tccaaaatga acttgaccgt aaacgaatgc catatcaacg
      541 atttgaaagg aaaaaaaggc aataatcatt tataccgaaa agtgagaatg tgtgaaataa
      601 ggacaaaaaa cctagtaact aagggaatgg ttcttatttc gcaccaatcg ctccaaccgc
      661 ccccgccgct taggtggtgc gaaataagcg ggcaacaccc gataacctaa gagaaagaag
      721 gagatccata tgaataaaat cattcctcta ttgttagtcc tatttgttgg cgaaatactt
      781 atgcgccatg ctgcagctgt gcatttaaac ttcgtagtat ttttggcaat actggtattt
      841 tgtttgatct tttggcgctt tgtattagcc cttgtgagtc tactattcaa gatgattgca
      901 gccatatttt tcatagggtt atttcgatga aaggagagag tactaatgcc aattgcaata
      961 ggaaataaac ggttacccgt tacgttggat gaaaagagac aaaaagaatt gcagcaacta
     1021 aagcagaagt acggcaaaag tgaatccagg attatgtgta ttgcgttaga tttattgatt
     1081 gcccaagaaa aagcaggatt tgaggtacca gcactcaaaa agtgacgtca ctttttatcc
     1141 taaaaactaa aagtgatagc acttttaatt ataagaagtt agaatattaa tcatttgctt
     1201 aattgtacaa tataatgtac aattgtttta tagaaataaa taaggggtga aaggaatgga
     1261 agcagtagct tattcaaatt tccgccaaaa tttacgtagt tatatgaaac aagttaatga
     1321 ggatgctgaa acacttattg taacaagtaa agatgtagaa gatacagttg ttgtattatc
     1381 aaaaagagat tatgattcta tgcaagaaac gttgagaaca ctttctaata attacgtcat
     1441 ggaaaaaatt cgtcgaggag atgaacaatt ctccaaaggt gcatttaaaa cacatgactt
     1501 aatcgaggtt gaatctgatg attaaggctt ggtctgatga tgcttgggat gattatcttt
     1561 attggcatga gcaaggaaac aaaagcaata taaaaaagat taacaagtta ataaaagata
     1621 tcgatcgttc cccctttgct ggattaggaa aacctgagcc attaaagcat gatttatctg
     1681 gaaaatggtc cagaagaatt acagatgaac atagactgat atatagagtt gaaaatgaaa
     1741 cgatatttat ttattctgca aaagatcact attaaccaat cggaagtaag gaaagggtca
     1801 gaaacttaaa agtttttgat ccttatttta tttaccctag tcatttaaaa agctaatata
     1861 gcttagtgtt gattgttatt aatgaatgtg tttgttacgc gtattacgga tataaggtta
     1921 gtaaaatcat ttctaaagtt gaggaaaagt aaatataaat ggcttaaatt tcaacaattt
     1981 gaagttgaat agatatgtta taatactatt gtagtgtggg atgttagtta ctaaaggatg
     2041 acgcttatat atatgactga atagaataag caataggttt aataatctat tttaaatttt
     2101 ttgtactagt tttagttaat tagcaaaaac aacaaaaa
//
LOCUS       DAZUMV010000221         2101 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000221 DAZUMV010000000
VERSION     DAZUMV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.230"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACG8FK_002826"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACG8FK_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACG8FK_002827"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACG8FK_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACG8FK_002828"
     CDS             1898..2059
                     /locus_tag="ACG8FK_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098360.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          751 a          330 c          360 g          660 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 t
//
LOCUS       DAZUMV010000222         2092 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000222 DAZUMV010000000
VERSION     DAZUMV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.232"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8FK_002829"
     CDS             <1..375
                     /locus_tag="ACG8FK_002829"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5098361.1"
                     /translation="DDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPD
                     GKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEK
                     LGGKNDYKDEIVYVYDYEKGDK"
     gene            376..1083
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8FK_002830"
                     /pseudo
     CDS             376..1083
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8FK_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
     gene            1185..>2092
                     /locus_tag="ACG8FK_002831"
     CDS             1185..>2092
                     /locus_tag="ACG8FK_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFS5098362.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYI"
BASE COUNT          816 a          266 c          374 g          636 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      481 ggtagagata aaaaatatac attagaatca ttaaaaaaac attatacaga gccttgggaa
      541 gatgaagttt ttagagtaat tattgaatat aacaatgttc ctattggata tggacaaata
      601 tataaaatgt atgatgagtt atatactgat tatcattatc caaaaactga tgagatagtc
      661 tatggtatgg atcaatttat aggagagcca aattattgga gtaaaggaat tggtacaaga
      721 tatattaaat tgatttttga atttttgaaa aaagaaagaa atgctaatgc agttatttta
      781 gaccctcata aaaataatcc aagagcaata agggcatacc aaaaatctgg ttttagaatt
      841 attgaagatt tgccagaaca tgaattacac gagggcaaaa aagaagattg ttatttaatg
      901 gaatatagat atgatgataa tgccacaaat gttaaggcaa tgaaatattt aattgagcat
      961 tactttgata atttcaaagt agatagtatt gaaataatcg gtagtggtta tgatagtgtg
     1021 gcatatttag ttaataatga atacattttt aaaacaaaat ttagtactaa taagaaaaaa
     1081 ggtgataaag tccgtataat tgtgtaaaag taaaaaggcc atataacagt ccttttacgg
     1141 tacaatgttt ttaacgacaa aaacataccc aggaggactt ttacatgacc caagtacatt
     1201 ttacactgaa aagcgaagag attcaaagca ttattgaata ttctgtaaag gatgacgttt
     1261 ctaaaaatat tttaacaacg gtatttaatc aactaatgga aaatcaacga acagaatata
     1321 ttcaagcaaa agaatatgaa cgaacagaaa accgacaaag tcaacgaaat ggctattatg
     1381 agcgcagctt tacgacacgt gtaggcacgc tagaattaaa agtacccaga acacgtgatg
     1441 gccatttttc acccacagtg tttgaacgtt atcaacgaaa cgaaaaagcc ctcatggctt
     1501 caatgttgga aatgtatgta tcaggcgttt caactcgtaa agtatcaaaa attgtggaag
     1561 aactttgtgg taaatccgtc tctaagtcct tcgtttctag cttaacagaa cagctagaac
     1621 ctatggttaa cgagtggcag aatcgtttat tatcagaaaa aaattatcct tacttaatga
     1681 ccgatgtact ctatataaaa gtacgagaag aaaatcgagt actctcaaaa agctgtcata
     1741 tagcgattgg aataaccaaa gatggcgacc gtgaaattat cggcttcatg attcaaagtg
     1801 gcgaaagcga agagacctgg acaacatttt ttgaatacct aaaagaacgc ggtttacaag
     1861 gtacggaact cgttatttct gatgcgcaca aaggattagt ctctgccatt agaaaatcct
     1921 tcaccaacgt aagttggcaa agatgccaag ttcacttcct aagaaatatc tttaccacca
     1981 ttcctaaaaa aaattcaaaa tctttcagag aagctgttaa aggaattttt aagttcacag
     2041 atattaactt agcgcgtgag gctaaaaatc gattgattca tgattatatc ga
//
LOCUS       DAZUMV010000223         2074 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000223 DAZUMV010000000
VERSION     DAZUMV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.233"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..531
                     /locus_tag="ACG8FK_002832"
     CDS             <1..531
                     /locus_tag="ACG8FK_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098363.1"
                     /translation="SFFKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSILT
                     LAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSN
                     RRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFS
                     FAREDEDNPKTYDILA"
     gene            complement(592..957)
                     /locus_tag="ACG8FK_002833"
     CDS             complement(592..957)
                     /locus_tag="ACG8FK_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS5098364.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1033..1278)
                     /locus_tag="ACG8FK_002834"
     CDS             complement(1033..1278)
                     /locus_tag="ACG8FK_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5098365.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1348..1602)
                     /locus_tag="ACG8FK_002835"
     CDS             complement(1348..1602)
                     /locus_tag="ACG8FK_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098366.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1766..2071)
                     /locus_tag="ACG8FK_002836"
     CDS             complement(1766..2071)
                     /locus_tag="ACG8FK_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098367.1"
                     /translation="MPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLV
                     MMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          766 a          354 c          369 g          585 t
ORIGIN      
        1 agctttttta aagaatattt agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc
       61 gttattattt tagtctattt tcaggggggg aaccttatga taaaaaaact atttttacta
      121 tcaatattaa cgctagcaat ggcaggttgt tctgactctg gcttagcaac tgaactgatc
      181 acaaaatcaa ctgaagccac gacagaggag ttaaaatgtg gaaacgatca cagtgactac
      241 agaggcttaa atcgacgatg catgggaata ttaggcagaa gcaattgtaa gcatcgatgt
      301 agtaatcgaa gaaggtgtag aagaagcaac agaaaaatca aaagaaagat caaaagaagc
      361 aatctgtgct gtgcatcgta tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg
      421 agatgttcat ttatgtctag ggaagttggc gatgaaccaa aactagagta cgcttttagt
      481 tttgcacgtg aggatgaaga taaccctaaa acttacgaca ttttagcttg atctaagatg
      541 gttaaagctt tttaatgaag taagaggaga tagaaggctt ttctagttat attaggtaag
      601 ataaattcct ttttgctcca aatttgctaa acattctgct agataaactt catcgtgttc
      661 cggataaatc ggcacgttgc gctctacagt aggtaaatca aaaaatgctt cgcatgattt
      721 gccacgatac ataggatttt tccactctgg tgctgggctg taaccacgtt tttccatttc
      781 atccatgaca agctgatgat attggaataa tctataagga gaatagtgaa aaacataatc
      841 cacagtagca tgtttctttc cccagccatt tcctcttaat gcacagcatt cccgatgttg
      901 tcctaaaagc tgttgacgag gaagctttga aatcaatttt tcatgccata atcgcataga
      961 aatgtctcct ttatatatag attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa
     1021 ggctggaaaa aatcacttca gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac
     1081 ggaaatatcg taaaagtcag cgatatagat taattcaatg atgcttggaa tttcttctcc
     1141 ttcttcgtac ttacgatatt tgaataaagg aatacttaac gcgctagaaa cagctacttg
     1201 agataaagat cggccagttc ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat
     1261 agcttcttcg atactcattt aatcaccttt cattatagta gaatagttat aatgactata
     1321 gaaagctaca catgcaatca atagtactca atgtttttta tttaaacgta tcaaataaag
     1381 aatgacgatc aataaagaca aaaccagtcc tgataattct tctataaaaa cactatcggg
     1441 ttcaaagtag ttgtaattaa aaactttcca cataaagtag atagccggaa gagtcaaaat
     1501 agctaaataa aaccgttctt tgttccaatt cataagataa acaatacttg cgaccagtgc
     1561 gaccgcaggc acccaaaaca aaactctcac caacgtaatc atgtaacgga ctcctcaacg
     1621 attcatataa taaaagataa catgttattg catacatcac aaatgaaatg ctaggaaaat
     1681 aaaagaagaa tagatcaatg aaaaatgaaa aagcagaaca gttttctgct gataagcagg
     1741 aactgttccg ctgaatgtgt cttatctatc gagaaaaaat caatggattg atatctaagt
     1801 aattcaatac atataaaaag aggacgatcg cattcactaa aaaggaaaga atgatgatcc
     1861 catagcgaat ggaaacatat ttaacaaata cttttgtttg aatacgggca tatctttttc
     1921 ctttatagct gagcatcatg acaagaagca aaagggtaat cacaccttga aaaatcattc
     1981 caataaacag aattcctgag gtttgataat ttaattggag aaaataagct gctgccgcaa
     2041 ctaaatcgaa taaaatcgca ataaatggca aaat
//
LOCUS       DAZUMV010000224         2035 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000224 DAZUMV010000000
VERSION     DAZUMV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.235"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..1459)
                     /locus_tag="ACG8FK_002837"
     CDS             complement(68..1459)
                     /locus_tag="ACG8FK_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS5098368.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            1585..1995
                     /gene="tagD"
                     /locus_tag="ACG8FK_002838"
     CDS             1585..1995
                     /gene="tagD"
                     /locus_tag="ACG8FK_002838"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFS5098369.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
BASE COUNT          779 a          323 c          283 g          650 t
ORIGIN      
        1 aactataaaa tttgttgaag ttgggataac tagttttctt ttctacttta tatacctaaa
       61 aatagtttca attagttact ccttcttctt tagatatagt tttttggata gaacccagat
      121 atccccaaaa aataaatgaa cctatcgtac taaccaaaat gagttctgtg ataaataatg
      181 ccgaaactgc tattgtaaga atcattaaaa tagcaaaaaa gtgcttatct tttactagat
      241 ttggttgtgt gaaacaataa cgcaaagttt taaagcattg gataaataag aaggttaaca
      301 atgtcaatag acctaatcct cctgtagcag ccataactaa gaaaataaaa ttatgagcag
      361 ttaaagtatc ttttcccatt aatgtatatg gtagtttatc atgtgcgtat tcaatatagt
      421 atcctggaga accgccggat atcggcttac tcttgaatat ttcaattgca cttttccaaa
      481 gtccaaacct taaattagaa acatccccgc catcagtgtc agggcgtttt aaatcaactt
      541 tattgctgct agttgttcct gatgaatcag atatatcttt ttttatttct gtaatatgcg
      601 gaacatgtgc ccatgcttga gcagttatat ctgttacata atacatcgca attatactta
      661 gcaatcctac tactaatgca gttagccatc tttttaaagt attagtttta agaaaacgac
      721 tactataact tgtaaagaaa ctaaaaacaa aagttgctat taataaacaa actaatgtgg
      781 tgcgtgagcc cgataaagca ataaacgaga attgaataaa aatattaatt gcaaaaaata
      841 ttttaaatag aagatttaaa tgcttgtaag tgaccaaata atatatagca ataatgatga
      901 caattaatga tataaccgca ccaaaatttg gatctgtata tacaccaaat aaacggttgc
      961 ctaaccaccc taaacgaact gggtaatact tatattttat tggaattttt atgccatatt
     1021 gaatgacaaa ccagtataat gatactaaat tagctaaaaa ccataccaat gaaagtatcc
     1081 aagtaaatac tttgaaaaaa aagttactac tgtagtcttt gccaaactga taaatcataa
     1141 agaaaaagat taattgccac atgattaact tgatattttc gctatatcca tattttaaat
     1201 tcattaaact gcttactagc atcgcgccaa taaaagcaat taatagccaa tcatattttt
     1261 taagattttt ttctctaaat gcaataattg catctataat gatcaagaca actcccgcta
     1321 ttccagcagg ccaaaaaagt aaagtatcat atctagtagg taatagtaaa tacgggccta
     1381 ttgctcgtaa cattagaata aaaccaaaat aaataaaata ccaacctttc acatatttta
     1441 aaaatttttc attcaacatt tttcctccat ccacctattt ctcataaaca tttcttatcc
     1501 tttttaggat aaactggttc tttcaattct aacaaaaaaa tgctaaacta acaatgtata
     1561 ttaatgaatt tcgagaggtg aaagatgaaa agagtaatta cttacggtac ctttgatttg
     1621 cttcactacg ggcatatcaa tttactgaaa cgagcaaaac aatatggaga ttatcttatt
     1681 gttgcccttt ctacagatga attcaattgg aatgcgaaac aaaaaaaatg ttattttagt
     1741 tatgaaaaaa gaaaacagtt acttgaagct attcgctacg ttgatttagt tattcctgaa
     1801 gaaaattggg aacaaaaagt aaaagatata aaagaatacc atgcagatta tttcgtgatg
     1861 ggcgatgatt gggctggaca atttgatgaa cttgaacaac taacagatgc acaagtaatt
     1921 tatttaccaa gaacacctga gatctctaca actcaaataa agaacgaact taattcttta
     1981 aatagtgata aataatattt tttaaacaat gaaagaacaa tatatctgga taaga
//
LOCUS       DAZUMV010000225         2031 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000225 DAZUMV010000000
VERSION     DAZUMV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.236"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..1268
                     /locus_tag="ACG8FK_002839"
     CDS             75..1268
                     /locus_tag="ACG8FK_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5098370.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(1434..1856)
                     /locus_tag="ACG8FK_002840"
     CDS             complement(1434..1856)
                     /locus_tag="ACG8FK_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098371.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT          540 a          340 c          410 g          741 t
ORIGIN      
        1 aagtcttgtt tttttattct tttttggata tacttttaac aatttaggga aattttagga
       61 aaaggggtat aaaaatgaca acgtatcagc gaaagttatt attatcgaca tcggcgggaa
      121 ttgctttaga gaatatggat attatgtttt tagctttttc attatcttca atgattactt
      181 cttttcatat tagtggtacg caagcgggtt tcatttcaac gatcactaat ttggggatgc
      241 tcattggagg tatttttttt ggcatcatag ctgataaata tggacgtgtg aaagtatttt
      301 ctcaaacggt ccttctgttt tcgattgctt ctttattgat gtattttgct tcaaacatct
      361 acttggttta tctctttcgc tttatcgcag gtgtaggagc gggaggagaa tacggggcgt
      421 gtatgtcctt gatttccgaa agtttttcaa aaaaacaaat tggacgagct tcttcggttg
      481 ctggtattgg tgcgcaggtc ggtgcggctt tggctgcgat tttagctgct gtgatcattc
      541 caacgtttgg ttggaagatg ttgtatgtcg taggtgtact gccggtgttg atggtcttgt
      601 tcattcgtcg cggcttgact gaaccaaagg aattcaaatc tgtacaatct gccgaaaaga
      661 aaaccaaact tagcagactg tttgaatcaa aacatcttgc ttggcaaaca tgtggcctta
      721 gtatcatggt aactgtccaa attgccggct attttgggtt aatgaattgg ctgccttcaa
      781 ttatgcaaaa tcgattaggg ctttcggttt ctgggtcttc attgtggatg gtcagtacta
      841 ttttaggcat gtctttgggg atgatgactt ttggcataat catggataaa ttggggccta
      901 aactagcctt ttctttgttt ttaatttgtt catccttatc tgtctatcta cttgtgttag
      961 cccatagcca gtggagtttg gtagcagccg ctgtgatagt cggctacttt atcaatggca
     1021 tgtatggtgg atatggcgca gtgattagta gtttgtatcc aacagaaatc cgcgcgacgg
     1081 cgaataattt cattatgaac cttggacgag ctgttggtgg tttttcttct atcgtcatcg
     1141 gtttcttgat ggatcattat agtttaactg ccgtaatctt gtttttgagc ggtatttacc
     1201 tagtcagtct gctggtcttg acaactctta ctggtgttac agcattaagg gaaagcttgc
     1261 agcattgata gttgagtctg taaatgacaa gaaagaggtg tggcaaaagc ttgcatctgc
     1321 ggtaataaga ccagccaaat caaaaacatg agacactgtt tccgtttggc tggtcgtatt
     1381 acttgatgca gggcagcttt ttcaactgct aatttccttc tatctggcgg ctactattct
     1441 ttgacgtccg tcatttcaaa gtactttact aataacttag cataacgaaa aactgtggat
     1501 acactcgtat attgagcgtc tgcataatct tccaaatttt tctcaggatg ttgatacatg
     1561 aattcaatca aatgataatt atatgatttt ctgtagaaat gaagataaat aatgtagatt
     1621 gggaagttct cctgcttttc catagaaaag cgccgattat tctcattctt caaaatgacc
     1681 ttatcaggat atttttggaa caattcttct aaatctgtta gataattcaa gatagactta
     1741 cttgatacct tcaattcgtt ttgtagttct tctattgaac accaacgttc tgtttcaagc
     1801 agtaaatgaa tcattttaat tttcttttga gaaacttttg ttaaaaaagt ctgcatattt
     1861 tctcctcctg tatctacttt actatactac atacaagaaa aaaaacggat acaaatatca
     1921 tattgtcaca ccgtcaatag tgcataaacc tgttattatt tctctttttt tcctaagtat
     1981 tctattgata tgaatcattt aatcgtttat tatataaatg tcaataatct a
//
LOCUS       DAZUMV010000226         2013 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000226 DAZUMV010000000
VERSION     DAZUMV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.237"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..694
                     /locus_tag="ACG8FK_002841"
     CDS             287..694
                     /locus_tag="ACG8FK_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098372.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVNLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="ACG8FK_002842"
     CDS             708..1109
                     /locus_tag="ACG8FK_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098373.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="ACG8FK_002843"
     CDS             1111..1482
                     /locus_tag="ACG8FK_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098374.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="ACG8FK_002844"
     CDS             complement(1518..1820)
                     /locus_tag="ACG8FK_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5098375.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          762 a          270 c          335 g          646 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taaacttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtg
//
LOCUS       DAZUMV010000227         2002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000227 DAZUMV010000000
VERSION     DAZUMV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.238"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..377
                     /locus_tag="ACG8FK_002845"
     CDS             <1..377
                     /locus_tag="ACG8FK_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS5098376.1"
                     /translation="NVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFRR
                     FPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTDD
                     WQKVMAAEVIATYLDGDLVYKK"
     gene            425..1825
                     /locus_tag="ACG8FK_002846"
     CDS             425..1825
                     /locus_tag="ACG8FK_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5098377.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaatgtttc cacagaggac ttctttaatt attgtcgatt agaggaaagt ggtgtaacag
       61 tcactagcgg tacagattta ccgctgttta ttactgatat tcctgaatcg attataagag
      121 cagtattccg tcgttttcct gaaggagaag aagtctggga gaaagaaaca agcttcacga
      181 caaccgagct gctgaaaagt tttacaagca acagcttcaa agtaaatggt ttatcggagt
      241 acggagaaat aaaagtaggg aaaagcgcca gcttagctgt tttcagcgag gacctctgta
      301 ctgatgactg gcaaaaagtg atggctgcgg aagttatcgc tacctatctt gatggtgact
      361 tagtttacaa gaaataatgc ttttcgatat aacgttataa aacgaattaa agagaggtta
      421 aaaaatggaa acaatgaaaa gaaaattcac cttcccacat agttatacat tgatttttat
      481 cttaattatt ttggctgcaa ttttaacttg gataattcct agtggtcagt ttgagacagt
      541 tgttgaaaac gtcgcaggta tggaaaaaac aacggttgtt ccgggaacct atcaccaaat
      601 cgataaggca gattatggac aaggtttttc ctctatacta atggcaccag ctgaagggat
      661 tatttcagct gttgaggttg ttgcttttgt tctcatcgtt ggtggtgcct tcgggattat
      721 cctaaaaacg ggagccatcg atcgagggct gtttacctta gcaaaagttc tgggagacaa
      781 ggggatctta gttattccga tgtcgatgat tctattcagc ttaggaggat caacctttgg
      841 catgagcgag gaagtcattc ccttatttgc ggtgtttatt tctctgatgt tttctcttgg
      901 ctatgattcc atgactgcga tcttgattct atttttaagt acacaagcgg gatatgttgg
      961 gtcgacgatt aatcccttca acgttttaat cgcacaggga gtagctggcg tttacggaaa
     1021 tcccctgctt tggtttcgga tgctatcctg gattgtcatt actgggattt caattgtttt
     1081 caccatgctt tatgctagca gagtcaaaaa gaatccactg tcctctattg tttatgagag
     1141 tgatttaaag gttaagcata agtttcaaaa ttttgacact gaaaaagagt tttcaacaca
     1201 agataaacta attatcgccg gttttatcgt aggtttagga atcatgattt ggggtatcat
     1261 ttccaaggga tggtatatgg ttgaaatttc tgcactattc ttaggattag gtctttttgc
     1321 tgggggcatc gctagatttt ctcaaaagga aattgctgaa gcttttgtcg aaggctgttc
     1381 agatttcgcc tatgctgctg taatcattgg gctagctaga ggagtcttgg taatcttgga
     1441 aggtggaatg attattgaca ctattctaaa cagcttggct aaccttttag gtggtcttcc
     1501 aaaattcctg ttcactacga ttctactcat ttctcaagta attgtttcct tttttgttcc
     1561 atcctcctca ggagctgcgg cattaacgat gccggttatg gcaccattgg gtgatctaat
     1621 gaatgtaagt cgtgatgttg ttgttctaag taaccagttt ggaaatggtt taatgaatat
     1681 tatctcccca actagtggtg tattactggc aggattagcc atcgcagaga ttaatttctc
     1741 taaatggttt aaggttggct tcaaaatatt tgccatctta acccttgttt ctgctgtttt
     1801 gttatacatt gctacactct tttagtctta atcatccatt aataggaggc aatagttatg
     1861 attaaaataa atcaattagc aggaatgaat atccactatt tattttattc tctcaaagaa
     1921 tttttcgctt atcaacagaa aattggtatt tcttctgttg aactttgggg aggtgctccg
     1981 cattttttca tagatgcttt ta
//
LOCUS       DAZUMV010000228         1971 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000228 DAZUMV010000000
VERSION     DAZUMV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.241"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..1355
                     /locus_tag="ACG8FK_002847"
     CDS             129..1355
                     /locus_tag="ACG8FK_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5098378.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFAGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(1463..1933)
                     /locus_tag="ACG8FK_002848"
     CDS             complement(1463..1933)
                     /locus_tag="ACG8FK_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS5098379.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT          738 a          307 c          381 g          545 t
ORIGIN      
        1 aaattttaaa aaataataat cgttacacaa aaatgttttt taaagttttc ttatgattaa
       61 aaagtgaatt tgctgattga acagttatat gagacaatat tttagaaaat gtgaaacaga
      121 ggtgtattat gaagcgatgg caaaaagttc tattaacctt gttaggggta ttcgttttgc
      181 tgattgccgg attatcagca tacggaatca gatttatggg agaagcgagc caaacagtaa
      241 acaaaatttc taaaaactct aatcgaatat cctccaaacg ttctgagaga gtaagcattg
      301 atgacaaaga acctttttcg gttttgctat taggtttaga taccggtgga ttaggaagaa
      361 cggaacaagg acgttctgat acgatgatgg tagtaacagt taatccgaaa caaaaaaaat
      421 caacgatcat cagtttggac cgagatattt atacgaatat cgtaggttat ggaacggtag
      481 ataaattaaa tcatgcctac gcatttgctg gtgtcgaaat ggccatggat tcaattgagc
      541 aactgctcga tattcctatt gatcattatg taacgatcaa tttggatggc atggaagatt
      601 tgatcaatgc ggtcggtggc atcaaagtga ataataaaat tgactttact ttggatggtg
      661 tccatgtacc agtaggtgaa caaacattga atggagaaaa aggtttagcc tattctcgaa
      721 tgcgacacga agatccagaa ggcgatatcg gtcgacaacg tcgtcagcgg gaagtagtga
      781 cgaaaatcgt caataaagtt ttaagtttag atggcgtaag caattataga aaaattttaa
      841 aagcagttga gaacaatgtg acgacggact tagattggga tgacatgctt gatgtagcga
      901 cgaactacac cccggcgttt gaaacaatca aacaagacca gttgcaaggc gaaggacaaa
      961 tgatcaatga tatctactat caaattttag gaaaaaatga tcttctttcg atccaaaatg
     1021 aactgaagaa acaattaaat ataaaaacaa gcgatacatt acctaatttg aaaaatgaga
     1081 atgcttcaat gatgttcttt gatgattcgg aagatggcga ttcgacaaac ggtgatggat
     1141 cgaatagtga agggtacacc gattattctt cctattcaga aaacacatat gatcagtacc
     1201 aagatccgaa tacctattat cagcaaacag aagatacaag tcagtactat acaccaagtc
     1261 aagatactca aagttatgat ccttacgctg gacaggaaca aaatggttat ggttatgacc
     1321 agaataatga gtatggaaca acaagtgggt actagaaaaa aatagtttgg ttaataaaga
     1381 gtgccaccta aattagacaa aaaatctaac ttaggtggca ctttttctat gtgttaaatt
     1441 acaggacgtc tctttttagc gcttagttta aaaaataaat tgaataattc aatagagttg
     1501 cgaatagtac ccataaataa taaggaatca tgagccacat tgccaggcga tgatgcagcc
     1561 acaactgata cataagcact gacaagaaaa taactaaaag tgtaatatcg attgctgcaa
     1621 tgaaaatatt tcttaaatta aaaaatagag cagaccatat aaaattaaaa atgaactgaa
     1681 tcacaaaaag accgacaaga ttgtttcgct gttttttgtt gttgtgatta agtaacaaat
     1741 aaagagaaat acccatcaat atatacagta aggtccacat cggcccaaat atccagctag
     1801 gtggtgcaaa tggtggaagt tgcaaagaat agtaatattt tccgggattt ccgctaagca
     1861 accctgataa aaagcctaat atgacaattc ctataacaca aacccaaaaa cgaatatctt
     1921 ttatcatctt cattttacta actcctttct ataatattag tttaccatga a
//
LOCUS       DAZUMV010000229         1966 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000229 DAZUMV010000000
VERSION     DAZUMV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.242"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..1033)
                     /gene="cysK"
                     /locus_tag="ACG8FK_002849"
     CDS             complement(107..1033)
                     /gene="cysK"
                     /locus_tag="ACG8FK_002849"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS5098380.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLGTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1173..1966)
                     /locus_tag="ACG8FK_002850"
                     /pseudo
     CDS             complement(1173..1966)
                     /locus_tag="ACG8FK_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
BASE COUNT          536 a          447 c          335 g          648 t
ORIGIN      
        1 attttacgtc tctattcaaa taatttttct catcataaat ttttgtgtgt tcttttcatc
       61 tttaaaagtt ttttacataa acctatctgg agaaaaacca agtgccttat atctcttggt
      121 aaagggctgt agataaataa cgttcaccat tgtcgggaac cacagctaat accttcttgc
      181 cttttcctaa ttcttttgcg acttttagcg cggcagcaat tgcagcgcca gatgagatgc
      241 ctactaaaat cccttctttt cgcccaactt ctctagctgt ttccatcgct tcgtctccac
      301 taatagaaag gactttatca tacacagaag tacccagtgt tttaggaaca aaacctgtac
      361 cgattccttg gattttgtga ggtcccgctt ctttcccttc taaaatagca gattctgctg
      421 gttctacccc gattaattcg attttcggat acacacgttt taattctcgg ccagcgccag
      481 tgatcgttcc tcctgtaccg atacctgcaa caaacgcgtc caagccattt actccaaatg
      541 cttgatgtat ctcaggacca gtcgtcttct catgcacaag aggatttgct tcattttcaa
      601 attgtaatgg taaaaagtag ccgttttctt ttgccaaacg ttctgcttct ctgattgatc
      661 cagaaatacc atccgcacca ggagtcaaaa tcaactctgc gccgtaccct ttcatcaata
      721 aacgtctttc gatactcatc gtctcaggca tcacgatgat tactttatat ccttttgcaa
      781 ctcctaccat cgacaatccg attcctgtat ttccagaagt aggttcaatg atagtatcac
      841 ctggtttcag tagaccatcg tgttctgctt tttctatcat acttaaagca atacgatctt
      901 ttacacttcc tcctggatta aaaaactcaa gctttacaaa aacatctgct gaatcttctg
      961 gtacgatttt atttaatttg acgattggcg ttttgccaat caattcggtg actgagttat
     1021 agatagctgt catcttcaat tcctccttgt ctttgctctt cttacttatc ataatacaat
     1081 ctgttcattt atggcgagtg attcgtcttt gactagcaaa aaaaagacaa agatatacca
     1141 tatatggtag tttctttgcc tttttatcta aatcaaacgt ttagttcttg accaactaat
     1201 aaagaataac tggacataga catgccattt ttcgccatta gatcatcaac agacatgcca
     1261 aatttttgtg caatatccca tagcgtatca cctgacacga ctgtatatgt ctgaccatta
     1321 tttgtattta catctgttgt tgaagatgat gacgttgaag tcgtcgtagt agtcgttgtt
     1381 gttgtacttg tggtagccga acctgttgat ggcgtatcaa aacgggttaa attataagta
     1441 ttaatcaatg agttcaattt ggccccataa tttggatcgg tagcataacg acctgtcaag
     1501 tacgctgttg catcataaaa acttgttgta ttgcttttcc acacgccagc ataatgataa
     1561 tcgcccgtgg aaaaactggt tgaacgaaga acattcgcat ggtcttggaa ggattcccaa
     1621 taagaagaat attttcggaa tggttcattc atcgttaccc attgtccgtt acatattctt
     1681 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
     1741 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
     1801 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
     1861 cgataaacgc ttgaggatca gcaactgttt gagatccttg ttcatctgca tgaacatgtt
     1921 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttcc
//
LOCUS       DAZUMV010000230         1929 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000230 DAZUMV010000000
VERSION     DAZUMV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.243"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG8FK_002851"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG8FK_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5098381.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG8FK_002852"
     CDS             852..1040
                     /locus_tag="ACG8FK_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5098382.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG8FK_002853"
     CDS             1053..1610
                     /locus_tag="ACG8FK_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5098383.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG8FK_002854"
     CDS             1642..1884
                     /locus_tag="ACG8FK_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5098384.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          710 a          296 c          397 g          526 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgag
//
LOCUS       DAZUMV010000231         1928 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000231 DAZUMV010000000
VERSION     DAZUMV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.244"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            198..1163
                     /locus_tag="ACG8FK_002855"
     CDS             198..1163
                     /locus_tag="ACG8FK_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5098385.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNVQNLNTPLKLQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMQLNEGNGFVKSVGKNGWQIVDQYEENIAYIELLQF
                     HEGKARIDFNPNKIGQFLASSMKNFIHDLFLEPHFSRADIACDMVNVPDEFIMQYRVV
                     DPVSFKPIYGRSGKLESAYWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQ
                     LRRGKATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYK
                     YRELLKEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
     gene            1363..1545
                     /locus_tag="ACG8FK_002856"
                     /pseudo
     CDS             1363..1545
                     /locus_tag="ACG8FK_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          630 a          294 c          438 g          566 t
ORIGIN      
        1 aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta cttcacctag
       61 ttccgtaact caagttcatt atacgaatat ttttaggttt cttcaagcta gagatttttg
      121 gatgtggatt tttagagtta cgtgaactct ggaaattctt tccaagaatc tcttttttcg
      181 ttgaggagga gctatttttg agtaaaaaag gcataatcgt aaatgagtgg gaaaaactta
      241 accgtatttc taacaggcgg ttaaatgttc aaaatttgaa caccccccta aaattacagt
      301 ggtcaatcga ccgaattacc attgttggaa aattgaaaga aaatatttat tatcacacac
      361 caaatgatgt cttgatttta aattttgagc aattgatgca attgaatgag gggaatgggt
      421 ttgtcaaatc agttggtaaa aatggctggc aaattgtcga ccaatacgaa gaaaatatcg
      481 cgtatattga gctcttgcaa tttcatgaag ggaaagcacg aattgatttt aatcccaata
      541 aaattggtca gtttttagcc agttcgatga aaaatttcat tcatgatttg tttttagaac
      601 cccatttttc gcgggcagat atcgcttgcg atatggttaa tgttccggat gagtttatta
      661 tgcaatatcg tgtggttgac ccggtatcgt tcaaaccgat ttacgggcgt tctggaaaac
      721 tagaatcagc ttattggggt agccgtgcca gcgaacgaca aattcggcta tacaataaaa
      781 aattggaaca agaaaccaaa cgaaaagttg taccaaagga agttgagact tggtggcggt
      841 tagaaatgca actacggcgt ggcaaggcaa ccgattggca taatatggtt caagaaagtt
      901 tgaatagttt tgcgagcctg cattttttgc cgattgatac aacacctaca gatagaataa
      961 tgctcgctgg tttaatgtct gatcaaaatt tctggtctct tttgtcgagg aattcaaaat
     1021 ataaatatcg ggaattgctg aaagaagaaa gtcaaaatga tgaattgacg aatcatttgc
     1081 gagaaacgtt tgcagaatcg gcagacaatt tgaaacaaga attagacacg tggcttttgg
     1141 gtttggacgt gacagagaag tgataccgaa taagaaaagt cagatgagtg agagtaaatt
     1201 tacgtcaatg tttaatgacg tgagtttacg ataagcgatt attttgcgtg aagcaaaata
     1261 aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt
     1321 gacagcgaat accctcggag agcatttgga attgtgacag ccatgtcaca attaggaaaa
     1381 tgttactctt tgtcaaagag taacagaaag gtttacactc tttttattag cgacagcgcg
     1441 aaggaggggt ttaacttggg gtttagtatt tcgttcaaaa agggaacaaa aaatacaagc
     1501 atttctcata ataatcgtga gttaactgat agccaaaaaa ttaatagttt gtctggtaga
     1561 aaataattag cagatttaca agtgggaaat aaaaagctga aagaagaaaa ttgcatttca
     1621 aactacattt tctacaataa ctctaaaatg gcatattttg cgttctgagc ggttttagcg
     1681 tttatttcgt ttagttatcg gcacaatcgg tgaaacacgt tagaggagcg aaaatggagc
     1741 ctgtaaggcg actggtagcg gaacggtcgt gttagattgt gtagccgata gctgaacgaa
     1801 ataaatgctg gcagcgccat tctatttcgg caggaggagg ctcaaggggt tcggggaatg
     1861 aaattccctg aggtcttttt attttttcta gaggagtgct ataatcaaag aaaatgagaa
     1921 aaaagagt
//
LOCUS       DAZUMV010000232         1829 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000232 DAZUMV010000000
VERSION     DAZUMV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.245"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..625
                     /locus_tag="ACG8FK_002857"
     CDS             <1..625
                     /locus_tag="ACG8FK_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HFS5098386.1"
                     /translation="NEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHP
                     NKAITVINRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGN
                     SDHYIHYDEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYI
                     INTENITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            801..1544
                     /locus_tag="ACG8FK_002858"
     CDS             801..1544
                     /locus_tag="ACG8FK_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5098387.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1537..>1829
                     /locus_tag="ACG8FK_002859"
     CDS             1537..>1829
                     /locus_tag="ACG8FK_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS5098388.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGN"
BASE COUNT          683 a          260 c          342 g          544 t
ORIGIN      
        1 taatgaaatc tattcctcag atattttttt cctagacatc gatttgaacg cctattatag
       61 cggtatcgaa ctaggcgaaa tgcttagaga aaagaatgaa aagtgtaaaa ttgtttatct
      121 aacgtctcat ccgaataaag caataactgt tattaatcga aagattacgc ctagtggtta
      181 tttgataaaa gaattcactt atgaaaaaaa taaaaaaagc atccaaagtt acttattgaa
      241 agaaaaagaa aactatcctg ttcaacatgc tacgaaaaaa aggtggacaa ctgtccgtta
      301 tggtaatagc gatcactata tccattacga tgaaatcctt tatctaatga gcatgccagg
      361 ttataaaaat aaaataggcc tagtattgaa agatgaagag attctcgtaa acggcacaat
      421 taaaaaatat aaaaaatcac tcgaatatga gtatttatgt aaagatttaa aagcttatat
      481 cattaatact gaaaacatta catctattag cagagtaaac gaaatagtaa catttgcaaa
      541 tggagaacaa ctagagatgg gaaagagaag tattgataaa ctaaagaatt ttatgcaaga
      601 aaagatagaa aatgaatcat tttaaagaaa acaaagactc atatcttacc tgttagaaga
      661 gtgttttcct gtagaagaga acaacccaag tagcagccta gtcgtttcta tccttttttg
      721 ttatgcactc tttttgacat ttactccacc aataacgaag ggtacaataa aaagtaataa
      781 caaacaaaag gagtacagaa atgattgaat tcatcaatgt aagtaaaagt tacgggaaaa
      841 aacaagcatt aaaagacttg tctctctcga taagacaagg ggaaattttt ggatttttag
      901 gacataatgg tgccggaaaa tcaactacga ttaaaagttt agttagtatt attgaaccat
      961 catcgggaac gattttagta gatggaatga aattgacgga aaatcgattg agtatcaaac
     1021 aaaaaatcgg ttatgtgcct gattccccag atattttttt acaattgacg gctggagaat
     1081 attgggactt gatttcagct gcctatgaat tagacacaca aaaaaaagaa aagcgtttag
     1141 cagaattgac agcgcttttt gatatgtata gtcatcaaaa tgaaacaatc gcaagttttt
     1201 cacatggaat gcgtcaaaaa acgattttga tcggcacgtt gctgccagat ccagatattt
     1261 gggtattgga tgaacctttg caaggacttg atcctcaagc agcatttgat ttgaaagaaa
     1321 tgatgaaggc tcatgccgca aaaggaaaaa cagtcatttt ttctacacat gtattggata
     1381 ccgcacaaca gttatgcgat aaactggcta ttttgaagaa aggtgaattg atttatcagg
     1441 gggcagttag tgaattattg aatggctcac ctgatgaaac gctagaaaag atttatttga
     1501 aaatggctgg aaggcaagcg acaggagatg gaccgtatgt ataagcgaca attattagaa
     1561 ttaattcgag ttaatctttt atatgttaat ccacagctca cgaataaagc aagagagaaa
     1621 ggaaaaaagg gaaagcgatt aactatctat cttttgtctc aaaatctttt ttcagggctg
     1681 atttttttac tgatttacgg atttgctatg tttgctatga actttgttaa attgccaggt
     1741 ttctttactt attacgtggc actttttggt atgttaggtt taactcaaag tatatcagtc
     1801 atttataata tcttttttga aggaaatga
//
LOCUS       DAZUMV010000233         1779 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000233 DAZUMV010000000
VERSION     DAZUMV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.246"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            611..943
                     /gene="traE"
                     /locus_tag="ACG8FK_002860"
     CDS             611..943
                     /gene="traE"
                     /locus_tag="ACG8FK_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS5098389.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1562..>1779
                     /locus_tag="ACG8FK_002861"
     CDS             1562..>1779
                     /locus_tag="ACG8FK_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS5098390.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
BASE COUNT          610 a          254 c          367 g          548 t
ORIGIN      
        1 ttataataat acgcgaagag aggtaatttc attttttaaa aaagctaaaa accttgatat
       61 tacttctttt caggcaatac gaatattatt cgcttacatt tttttgtata aatttagttg
      121 ttttaaattt aattgtctgc tacgcttgtg atgaattaat tttcgttctg cacttttgga
      181 cgataaattt ggacaattga aaatacaaaa aaaagaaagg ccctgtgaga ctcggaaggc
      241 tggtaacctt cgtcgacgtc cacaggtggc attaagacaa cctttaatcc cttaatgcgt
      301 ttacctttca gtgttacggt attacccgca acggatttag tataacagat attcaaaagt
      361 gataccactt tttttggctg tgttaaggtt aaaaatacgt gatattacga tgaatctatg
      421 cctaagcata atgttataaa aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa
      481 ttagagggaa cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga acgaaaatca
      541 attctctatt tttgaaaaat aacaagaaaa acttattaaa aatatttaaa gtaagtgggt
      601 ggattatttt atgtcatata caggaatttt gagtctcgaa gatatttgtc actacggtaa
      661 acggtgtacc gctacagaga aaatcacgaa gaaactatca actggccaaa gtaaaaccgt
      721 tgtgcagtgt aagaaatata taatccagaa agacaaagtc tcagaggaaa tgatttatta
      781 cgctggtaaa caaaagcaaa taattttaaa agatcctatc cctttaaaag aactatatcc
      841 aacaatcaaa catgtttatg accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt
      901 cttgcgttgt accgcaaaag ggatggggcg tttaatcagc tgaattgatc attctcataa
      961 cttgtatttt atcgtttttc gcttgcttga aaatattgtc gagacttcta aaagaactta
     1021 gaattactag aaaaataagg agtagacttg atctatcaac aattcctttc ggggggtgac
     1081 ggggtaggtt tttacgattg gtaacgtaaa aaattggtca atcgagtaaa tgcccttgtt
     1141 tggatagtgg tgaaatgaat ttgtgtaaat aatatgcaca gagtaaacaa gagggaattt
     1201 agaagttaga caagttagaa tagcgcctag attaatcatg agcagctact aagggatcta
     1261 ggctttgacg caaactcatg gaataaggag taggtcttct cgtaatgggg aggacgcaca
     1321 agaaattgtg tcgtgtgctg cagtacatgt agatacttgc gaggacgttg gtcattggat
     1381 caatcgagca tttaccgatt tctactaggg tttctcgttt cggacgccat aaacgagtcg
     1441 atatttttta tgtattttta agtaaacgaa aaacgataaa atatgaacga gtcctgaata
     1501 aagtgaaaaa attgatataa aacatagaga aaatgaaatt tacgtaatgg aggaatgaca
     1561 aatgaaaaat tatgatccga atatacgatt gggcacacat actattaagg tttctttcca
     1621 acgttgggat tacaaaggat ttgtgacgtt tagaagaggc ggaaattgta aaggattaga
     1681 tgttttagca ttagacgagg atgatttata ctatcaaaaa ttgactgata atccaattgg
     1741 attcggcttg ctacctgaag atgatgaagg taatgaatg
//
LOCUS       DAZUMV010000234         1671 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000234 DAZUMV010000000
VERSION     DAZUMV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.247"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..326
                     /locus_tag="ACG8FK_002862"
     CDS             66..326
                     /locus_tag="ACG8FK_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098391.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(688..927)
                     /locus_tag="ACG8FK_002863"
     CDS             complement(688..927)
                     /locus_tag="ACG8FK_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098392.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1213..1527
                     /locus_tag="ACG8FK_002864"
     CDS             1213..1527
                     /locus_tag="ACG8FK_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098393.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          580 a          223 c          288 g          580 t
ORIGIN      
        1 tcataatcat attttaatgt tattatcatt ttaaataatg aaaaaacatt aaggatggat
       61 gaaatatgaa taacgaaact atcattttag gaaaaaaata tatttgccaa cctattggct
      121 tgaaacaaaa agtgattgga gaagtaatca gtaaattaga aaattgtgtt gtgatctgcg
      181 tagacgaata tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga aaagtagtgg
      241 caaaatatac tcacgtttat gagttggaaa caacagaata tctatataac tatagcaaca
      301 aatatgaaca tactttagtt tcataaagat ttaaataaag tgccttcaaa agcgagaaga
      361 ccaagctttt gaaggcactt tatttagttg gcagtgattg ttcatgattt ttgatttatt
      421 caaaaggttc gcctagagaa tctgtctctg taacgaggcc tggtggagaa actcgataaa
      481 aacctatact gccagaactg tcctattttc ggatagacaa actagtagtt ttaaaaagat
      541 catccgatcc aatcatttca aaaaatgcaa aactgatatc aggatcattt agctcaggct
      601 tgttatcgta aagcatttgt aaaggttgca tttagttagg agtaaacgcg aaactatcat
      661 cattatttgc tgaagaagat gaagaagcta attcagagtt tgtttgaaat aaaggagacg
      721 tgctgacagt ttctttaccc tgttttttgc agaggtagaa acttttatat cggaagtttc
      781 taccggagaa tttttctcat ttgtagaaat tgttgaatcg atactattcg aaatttgcgt
      841 atctgaaggt atgttctttg atgcagaagt cccacaagca gtaagtaaca ataaatttga
      901 taaactgata aacagaattc ttttcattat gtacacactc cttatagtat cgtttagcat
      961 acacctatgc tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt tatagtatct
     1021 attagtctta gtaatacata tatggagagg gtgatatccc agttcaaacc agatacattg
     1081 gttaccatct gtattaacga ttatgcctct aaacgctttt tttatgaaaa aatttataag
     1141 tgaaaacgta ggtatgatat tatctagagg taattttttt gggtatttaa atagttgcta
     1201 ggaggatagc ttttggattt ttacagtaat tttatcttaa taatagcaat attattactt
     1261 ctaaatattt ggttttttga taagagtaga aatgcaggga taggttttag aaccaaacga
     1321 agtacgtctt ctgaaaaaaa gtgggtatat tcacagacta ttttttacgg aggggttatt
     1381 tcaatcagtt tactttcttc aacactttat tcttttaatg ttatagacgt ttccatgtca
     1441 aattttatat ctattattgg aatccttatt tctgcaatta ttacacaact acttcttgtg
     1501 tttgaagaga agtcaaaaaa taattaaagc aattatgcct tttagtacag gaattattac
     1561 ttcttacaaa tgtatagcga atgtgaagcc tgctgtttta attgttctag aaaaactttt
     1621 gagtgaacaa ttattttatg ttaggatgaa ttaatttctc tagagtttat a
//
LOCUS       DAZUMV010000235         1666 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000235 DAZUMV010000000
VERSION     DAZUMV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.248"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1666
                     /locus_tag="ACG8FK_002865"
     CDS             <1..>1666
                     /locus_tag="ACG8FK_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFS5098394.1"
                     /translation="DDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFD
                     NEICASISLNSESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFN
                     EFRNSIRLSDYEFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDM
                     SDIFKHISVVYVDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNET
                     ITSVPEIRKIGENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLD
                     LLGLGHLNMIYLALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNY
                     TQILMTTHSVHLAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINA
                     VERYLDVKRNGLLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFE
                     YIASLFDDDRIQRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWV
                     NCFYANTTFEIEFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQ"
BASE COUNT          630 a          231 c          276 g          529 t
ORIGIN      
        1 agatgatagt tattactata gtagtaaaac actaaaagaa tcagattttt ttcatggaat
       61 tcaaaatggg tggcaagggc attggattat tatctctgcg acatttgaag gaatcagcga
      121 agaagaattt gacaatgaga tttgtgcttc catttcttta aattctgaaa gtcaaacaat
      181 cttagaagaa ttgatttcaa atgcagataa aggtgtaggt agtatttctc tatttattag
      241 acctaacaaa gctattagaa agcaattatt tgatatttcc actgaattag actctcatca
      301 atttaacgaa tttagaaatt ctattcgtct atccgactat gaattttact acacatctaa
      361 atcaaatcta aatttctgta tcaatgaaaa ttatgataaa ttggtaggaa gactcaatga
      421 caatatagct agtaatcctg aagaagacga tgaagctctg ttgggctcaa gaattgacat
      481 gtcagatatt ttcaagcata tttcggttgt ttatgttgac gctcttcgag atgttcttag
      541 agaaatgaaa aacaatcgaa acccagtcaa aagaatcatg gaaacaattg aatcaaaaat
      601 atcatcagat aatgttgagt cattaaaaac aattatacag caattaaatg agactattac
      661 cagtgttcct gaaattagga aaattggcga aaatattaat cggcaactaa atagcataat
      721 tggttcagtg tattctccta atcttcttct ttcctcaact atgtcagatg atatgggttc
      781 tttagcaaaa tttctttcaa tgaagccaga acaaaatatc gacttagact tactagggtt
      841 aggacatcta aatatgattt atttagcttt gaaaatagtc gaatttgaag cgtgtcgttc
      901 aagagaacta cttaatatta tgttgattga agaaccagaa gctcatattc atcatcatat
      961 tcaaaaaact ctgttcgaag gattaaatct tcaaaaaaac tatactcaga ttctcatgac
     1021 cacacattct gtacatttag ctgaagtgtc tgaaatatct cggatgaatg tattaaaatc
     1081 gtttaatggc aaatctatcg ttatgaatcc agcaaataat ctgaactcat ttgctaaaga
     1141 aaagttaaaa aagaacaatc taaatctaat aaacgcagta gagagatatt tagatgttaa
     1201 aagaaatgga ttgctttttt ctaaaggtgt tatcttagtt gaaggagatg ctgaagaaat
     1261 acttattcct caaatagtaa aaaaattatt tggtataaac ttagacgaac ttggaatagg
     1321 tttgattaat gttggtagta cttcgtttga atatatcgct agcttgtttg acgatgatag
     1381 aatacagaga aagtgtgcta ttgtaactga tttagatgaa caggcaatcc catcagaaca
     1441 tcgattgtat aaaagcactg cacaagagaa aggtcaagca agaaaagaaa aattatcctc
     1501 cctttttaat gaaaacaatt gggttaattg tttttatgct aatacaacat ttgaaattga
     1561 gtttttagat gtgaataaag aaaatattct aacttatgtc agtccaatta tagatgacgc
     1621 attcaaagat aaaaaaacaa taactgctta taaagaagaa atacaa
//
LOCUS       DAZUMV010000236         1664 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000236 DAZUMV010000000
VERSION     DAZUMV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.249"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8FK_002866"
     CDS             131..505
                     /locus_tag="ACG8FK_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5098395.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8FK_002867"
     CDS             complement(619..1248)
                     /locus_tag="ACG8FK_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5098396.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8FK_002868"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8FK_002868"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZUMV010000237         1631 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000237 DAZUMV010000000
VERSION     DAZUMV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.250"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..893
                     /locus_tag="ACG8FK_002869"
     CDS             36..893
                     /locus_tag="ACG8FK_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS5098397.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            913..1473
                     /locus_tag="ACG8FK_002870"
     CDS             913..1473
                     /locus_tag="ACG8FK_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFS5098398.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT          613 a          218 c          295 g          505 t
ORIGIN      
        1 attgcttgaa gtattatatt tagatatcat aatttatgga agaaaaatta attaaaaagg
       61 atgagattat aatggggtta ctggttccga gagctaatac ttatactata cctttaacga
      121 aacgccaatt aattgctaaa agatatcaaa ggattactag agccatcaat agagagtttt
      181 ggaattctga aagtgatact gctcacagtt tatacgtagg ttcttatggg agaggaacgg
      241 caattagtac tagtgatata gatataatag ttgaattacc gatggcagaa tttgacagat
      301 tcaaaaatta tttatcaaat ggcccgtcta agttattaca agttataaaa aacgcatttc
      361 aggaaatact accaaatagt gatattagag ctgatggaca agttgttaaa ataaatttcc
      421 atgatggaat caaatttgaa atagttccag cttttaatga aaaagattat tggggtgagt
      481 caaaagggtt tatttatcca gattcaaaca tgggagggaa ctggaaagct acaaatccca
      541 aaaaagagca agaagccatg aaattaaaga atacaaaaag taataattta ttatatgcta
      601 cgtgtaaaca ttttcgacat gtacgagata cagaatttac gagttatcat ctttcaggaa
      661 tagtaattga cagtttcgta tatgaggcta tgggaaattg gaaattcgtt gaaaataaca
      721 gtgggggtca aaacattagt tctgtatctt atgagacagc tttattagaa tactataact
      781 cacataaagt tatgggcggt ttaaatttat actctcctgg aagcaatcaa tttgttaatt
      841 ctgatagtag tatcatatgt ttagaaaaag tattaaaaaa aatagcttta taaagtttta
      901 tggaggaaca acatgggaga cgaaaaaaag cgcttggaag ttcaattacg tgaagcatat
      961 ggacgagtaa cgtatactta caccactcat ttaaagcaag ccgataatat tatttctgac
     1021 aataaaattt taaggtgtct tcaaattggt ttgtcagccc ttactactgg aggggtactt
     1081 agtaccatta ttactgataa tagtgagtta acctttgttg ctggattatg ttcaacaatt
     1141 tctcttggat taaacttata ctttaagaat tttgatcttg ctgaagaagc taaaaatcat
     1201 caaattgttg ccgataaatt atggttgatc agagaagaat acgtaagctt actaacagat
     1261 ttaaatcgtt taagtgttga agatattgtg aaaaaaagag atcaattaat gaacgaaact
     1321 catgaagcat ataaaaatgc ccctaaaaca aatagtaaaa gctatgaatc agctcagaaa
     1381 gcattaaaat ctgaagaaga gcaatttttt acagaagagg aattggataa gatgttacca
     1441 gagcatctta gaaaaaagag ttctaaatat tgaactcttt ttttatgctt aaaattctct
     1501 tgtaattttt ctattcatct atatttaaga agaaatatga tgatagaaac atctcctaaa
     1561 gccaccgcaa gccgttctaa gcatagctta gcccgacatt gctagatatg ctcatctcac
     1621 catgaacgtt t
//
LOCUS       DAZUMV010000238         1613 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000238 DAZUMV010000000
VERSION     DAZUMV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.252"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1186)
                     /locus_tag="ACG8FK_002871"
     CDS             complement(146..1186)
                     /locus_tag="ACG8FK_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5098399.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT          526 a          244 c          206 g          637 t
ORIGIN      
        1 ttgtgatttt tatcataata ttttaactca taaattctat taaaatagct atttctttgt
       61 aacatttgta atataattag aataataaaa aatttttaat tttaatgcta ctccatgaag
      121 caacaatcaa tttgtcagtg aaatttcact ctaaccagtc tttcgtaata gtttttggga
      181 agctttcaga attcaaacgt ttttcacttg atactttagc agctattggt ttttcgataa
      241 accaattttt tacatagaca aaaatcaggt tctggatatt ttcaactttt tcggttttta
      301 atttttgata ggcttttaac agtgtcgtgt ataattcttg ttctgcattg acataataac
      361 tatcgagttc ttcaaaaatt attgaaatac cactttctgt ttcggctttt ttctttgcat
      421 tatgaatagt ctggacagtt ttttgagctt cactgtaact tgaagaaaat gtttttatta
      481 aatttaatac tcgttcaggg ataaacgtag caatgctgtc gtttgtcata attgtctcaa
      541 aattgtctaa caacaattga tcttgtaggc ggtctttttc agtgtctata ttgtgtctat
      601 tagtgtctaa tatattatta ttaagttctg gtatgatttt cataccctct gatttatcaa
      661 cgttttggca cggtatgatt ttcataccct cttgagcccc aagggattca gcgtctgatt
      721 tttcgtcgag ggtgggtatg attttcatac cctctgattt atcaacgttt tccctattct
      781 ctctatcaaa tttttcgagt aagtagatat cattttctga gacttttaat tctgctagat
      841 atagtctatt aggattattt ttccctaact gtttgttaaa tcccatctgc ttttgtaata
      901 ataaaccaaa ggattccaac tcttttttga ttgctatcgc tttttgtttt tggcagttta
      961 atacgctaca tagttcattt gtcgtataag taaaataaac attaccttct tcatcgattt
     1021 gccgtttgct gattgcgatc tctaatcttg cttttaaaag catataggca atcttagttg
     1081 aatctttcat ttttttgtat tcgtcagaaa caataaagac ttttggaaat tgataaaata
     1141 attcgttgta aactctatcc gccttaaaaa aattaaaatc agtcattgta gatcctctac
     1201 tttcacgttg ttttatttat gtaaaattac ctactctttt cgaaaatagc aaagataaat
     1261 aaaaaatgca aacgcattaa aactgttttt taattaaata ctacctaaaa tcagaaataa
     1321 aatgaattat gtactacctt taaagtcatt aagaactact ttaaataaat attatttata
     1381 attaaatact accagaagaa cgtatgaata aaatttagtt ttaaaattaa agataattaa
     1441 gtactaccag tgtcaaaaca aaaaataaat taagaactac cttatgcata taaaacatac
     1501 taccattact agtaatatat actaccttat ttcagtagta tatattatat tcttcttata
     1561 agacaaggct ttaggctaga aaacaataga ttatatacta ctaaaaaata caa
//
LOCUS       DAZUMV010000239         1589 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000239 DAZUMV010000000
VERSION     DAZUMV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.253"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            791..>1589
                     /locus_tag="ACG8FK_002872"
     CDS             791..>1589
                     /locus_tag="ACG8FK_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5098400.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRD"
BASE COUNT          579 a          253 c          264 g          493 t
ORIGIN      
        1 gaagtaataa tatcgctact attaagataa aattactata aaaaagcaaa atattacctc
       61 ctaattaaat attaaatacc gaaaaaatta tgtttagata ataccatagt taagtttttc
      121 cttgtgatgt ttttcgtaaa aagatatttt agcagcatta tcgttacagt gcatagttac
      181 catcggcgtt atgactatat aaagtatact ttcgttgatc cagaaaggta tatagattaa
      241 aattagttac ctaaaaagga caaatacaaa ttttattatc ttattcacag taagaagata
      301 attttaagat tgcgaaatac gatactattt gtaatctata gtatcagtcc tataaaataa
      361 acatctaatt aattactcta aatcctaatt ataagccttt tttctcactt tttttcaaaa
      421 atatggtatg cttcttagta aattcaaata tatggtatgc ttcttagtga attcaaatat
      481 aaaagagttt aaagcgtaat acacttatgg ggaagtggtt tggggtgcgc tttaaactct
      541 tctttattat tatctcacag catgcttaaa aggtctatgg attaagttat gtaaaaaagt
      601 gcctctttaa acgtcctaaa atccaaacaa ggatagcata atgtacacta tcgttgatcc
      661 agaaaacagg taaataaatt ttgcaaaagc aataagtcgc cgattgtaaa attaagctag
      721 acaactaaaa aagtcatttg tgctacactc aaaatagttc cgaccaaaga attataagga
      781 gtgagtacaa atgacctata cccatcttac aacggatgaa cttgtaataa tagagtctta
      841 tttcaaaatg aatcaatctg ttgctaaaac tgcccattgc ttgaatcgtt caagacaaac
      901 gatccataaa gtatatctgt tcttcaagca aggaaaatca gccttagaat attatcaaca
      961 gtataagaaa aacaaatcaa actgtggtag acgtccgctt gttttacccg aggaacaatc
     1021 agaatatatt caaagaaagg ttgttcaagg atggacaccc gatgtgattg ttggtcgtgc
     1081 agcgtttcct attagttgtt ctgctcgtac catttatcgt atgttcaaaa aggggctatt
     1141 tgattcttct gacttaccga tgaaaggcaa gcgtaaaccg aatggacatc aagaaagacg
     1201 tggaaaacaa actttccgcc gctctattca tgaacgtgaa aaggattata gccaattctc
     1261 aaatgagttt ggtcaccttg aaggtgacac tatcgtaggt ctgaaacata aaagtgctgt
     1321 aattacctta gttgaacgat tatcaaaagt tatcatcaca ttgaaaccgt gtggtagaca
     1381 agcgattgat attgaaaaaa aattaaatca atggtttgaa tctgtaccga aaaacctatt
     1441 caaatccatc acttttgatt gtggaaagga attttcaaat tggaaacaga tcagtaatgt
     1501 caatgatatt gccatttatt tcgctgatcc aggaacgccg tctcaaagag gcctaaacga
     1561 gaattctaac ggattgttac gtagagatg
//
LOCUS       DAZUMV010000240         1588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000240 DAZUMV010000000
VERSION     DAZUMV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.254"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..652
                     /locus_tag="ACG8FK_002873"
     CDS             56..652
                     /locus_tag="ACG8FK_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5098401.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(710..1192)
                     /locus_tag="ACG8FK_002874"
     CDS             complement(710..1192)
                     /locus_tag="ACG8FK_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098402.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1298..1495)
                     /locus_tag="ACG8FK_002875"
     CDS             complement(1298..1495)
                     /locus_tag="ACG8FK_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098403.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          538 a          329 c          248 g          473 t
ORIGIN      
        1 catttcgtaa tcaattaaaa aaatgcaaaa cgcaaatcaa aggagaaatt ttattatgac
       61 aacttttact gacactttga aaaaccgccg ttcaatctat catctaggac gtaatgtttc
      121 actttctaat gaagaattaa caactttgat caaagaagca attaaagaaa gcccaactgc
      181 tttcaatgct caatcaacac gcgcagttat ccttttcgga gatgcacatg aaaaactttg
      241 ggaaatcact gaagaagcac ttcgcccatt gactccagca gaagctttcc caaatacaca
      301 aaacaaatta gctggtttca aaaatggtta tggtacagta ttattcttca aagatactga
      361 cgtggtaaaa ggtctacaag aacaatttga attatatgca gacaacttcc cagattggtc
      421 agaacaatca aacggaatcg caacagctaa tacttgggta gcattagttg ataaaggatt
      481 gggcgcaaac ttgcaacatt acaacccagt tattgacgaa gcagttgcaa aagaatggaa
      541 tatcccttca aactggaaac tacgttcgca attagtattc ggttcaccag aaacaccggc
      601 tggggaaaaa gaatacatga acgatgccga tcgcttccgc gtatttggat aattttattt
      661 gaaataaaaa acagaggttg tgacaaacat cttgtcgcaa cctctgtttt tattctggat
      721 caatcataat atcaatctct gcctcatcgt ttaagcgttg ggacaaaaat ggaactaatt
      781 cttctaatga tgcagaaaat ttcagcatcg gtcctcgcca tccgtttcgg aaaaaattca
      841 tttttacgat tttagaacct tttggctgat ataatacaat tttattgatc tcagaaaaag
      901 gaacaccttt tttgtcaaag ctattcaaaa caagtccttc ttctgtgatt cctctactgt
      961 ccaacaaaaa actaataaaa atcattgctg acaagatccc ttttgccaat tcatctgctg
     1021 taggatgact gatgagtgta taccaaatga ctagtgacac taagaagggt atcaccaagt
     1081 agacccatcg atttcgcaca gtccgataaa taatttcttt tcgaaccagc caaagaaata
     1141 aaaacatcac aacaacgaat aaaaacaata gaaccgtaaa gaatgttacc atgacaactc
     1201 gcctcgcttt ttcttctcca gaaaatagga aaagaatact gatttacctt ctcaaagaaa
     1261 ttggacttgc agcacgccgc acgtccaatt gttttcttta ttcaaatgta tcatattttg
     1321 caacaaccat gcttgctttt tcatctaata attcttggtc ctctgcagca cattgggcaa
     1381 ctttgaccac ggcgcagttt gtcatttttg atacaacttc accaatgacc gtttttgtaa
     1441 aaccaattgg tttgcatgca tattgttctc ctaaattaat catttcttcc gtcatcaaaa
     1501 tcctcctaaa ctgctggaac ttttctccac attaccactg ctatttcttt ggagatttaa
     1561 ctcattgatt tttatttaca gttaaaaa
//
LOCUS       DAZUMV010000241         1556 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000241 DAZUMV010000000
VERSION     DAZUMV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.255"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8FK_002876"
     CDS             132..344
                     /locus_tag="ACG8FK_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098404.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ACG8FK_002877"
     CDS             506..1024
                     /locus_tag="ACG8FK_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS5098405.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ACG8FK_002878"
     CDS             1031..1543
                     /locus_tag="ACG8FK_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098406.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          242 g          522 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agtttt
//
LOCUS       DAZUMV010000242         1550 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000242 DAZUMV010000000
VERSION     DAZUMV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.256"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1360
                     /locus_tag="ACG8FK_002879"
     rRNA            <1..1360
                     /locus_tag="ACG8FK_002879"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1455..>1550
                     /gene="rrf"
                     /locus_tag="ACG8FK_002880"
     rRNA            1455..>1550
                     /gene="rrf"
                     /locus_tag="ACG8FK_002880"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          432 a          331 c          459 g          328 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg acttaaccaa taggttaaaa gaaaagaact
     1381 cggaaaaaaa cttatgtgac ttcaaatcca gttttgagtg aacaaaattt actcaataaa
     1441 agataacacc acagtgtggt ggcgatagcg agaaggatac acctgtaacc atgccgaaca
     1501 cagaagttaa gcttcttagc gccgattgta gtgaggggtt gccccttgtg
//
LOCUS       DAZUMV010000243         1533 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000243 DAZUMV010000000
VERSION     DAZUMV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.257"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACG8FK_002881"
     CDS             complement(52..1518)
                     /locus_tag="ACG8FK_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS5098407.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          501 a          325 c          289 g          418 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aat
//
LOCUS       DAZUMV010000244         1533 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000244 DAZUMV010000000
VERSION     DAZUMV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.258"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..1252
                     /locus_tag="ACG8FK_002882"
     CDS             83..1252
                     /locus_tag="ACG8FK_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5098408.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          547 a          250 c          253 g          483 t
ORIGIN      
        1 aaatagaaat ttagggtaga aaaaaaagtc cattctgtaa aattaaagtt accacacaga
       61 aattttatag gaggactttc tcatgactaa atttactata gaaattatgc aaacactact
      121 caataaaggg gatttagatg aattatttca ggatcattta gagcgagcga ttaattcact
      181 tctacaagca gagctaactg cttttttaga ttacgaaaaa tatgatcgaa atggattcaa
      241 ttcaggaaat tcccgtaacg ggaattattc acgtacattt aaaacagaat atggcgagtt
      301 gaatttaact attcctagag atcggaatgg cgaattttct caacaaacac taccagcata
      361 taagcgaacc aatgattcat tagaaacgac cattatccaa ttatttcaaa aaggcattac
      421 tatggctgaa atctccaaat taattgaaaa aatgtatggt caccactata cgccacaaac
      481 aatctccaac atgagtaaat tggtggctga agatgtttta gcttttaaag aaagaacgtt
      541 agaagccaat tattccgtta tatttatgga tgcgacgcat atccctgtga agcgacagac
      601 tgtttcgaag gaagcagtgt atattacgat tggtattcgt ttggatggaa ccaaagaagt
      661 tcttgggttt actattgccc caactgagtc tgcttatatt tggaaagaag ttcttcaaga
      721 tcttagaaaa cgtgggttag aagaagtttt attagtagtg acagacggat taagctgtat
      781 tgaagaaagt atccatagtg tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt
      841 atctagaaat atcgctcata aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt
      901 caaattggtt taccaagcga attcaaaaga agaggcattg gatcacatcg actttatgac
      961 taggaaatgg aaaaagcagt atccaagagt cgtcaattta ctcttgaatc ctgccctatt
     1021 aaccttttat aatttccctc acgccatcag acgaacaatt tattcgacga acctgattga
     1081 aggctttaat aagcagctaa aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga
     1141 atctctagag agattcttca tttctcaatt taatcaatat aaccaaaaat ttttaggtag
     1201 aattcacaag ggatttaaag aaattcagga tacattagag tcgatgattt aactgtaatt
     1261 aacagaatgg attttccatt tacatataat tcttgacgct accataatct aaataaccat
     1321 catattcatc tactacttat ataattgagt tttattaaac tttttaagtt tctattctat
     1381 caaatattta aacaatctct gcatccgaaa agactacctc gtatagtttt actaacaata
     1441 tttttattag cgtgttcaaa tcctgatttc ttgtatcaat tattttctgt tcactttcaa
     1501 taagaaaaaa gtctttactt ccatggataa taa
//
LOCUS       DAZUMV010000245         1500 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000245 DAZUMV010000000
VERSION     DAZUMV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.259"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..443
                     /locus_tag="ACG8FK_002883"
     CDS             21..443
                     /locus_tag="ACG8FK_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098409.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            453..656
                     /locus_tag="ACG8FK_002884"
     CDS             453..656
                     /locus_tag="ACG8FK_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098410.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            867..1451
                     /locus_tag="ACG8FK_002885"
     CDS             867..1451
                     /locus_tag="ACG8FK_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5098411.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          533 a          218 c          282 g          467 t
ORIGIN      
        1 tgatttcgag gtgatagaca atgaaaaata agtttatttt atctttaggt attttaacta
       61 ttggaattag tgtaactgga actacaattc ctacatttgc tgatagtaca aatgaaccaa
      121 ctactgtagt agataataac caagaattaa ctcctttaga taggaacgga atagccattg
      181 gtaacccaac taacgactta actgttactg aaaattattt aatagtttct ccattacttg
      241 ctcgagcagc ttctaaaacg atacgagtga caggaacgat gatttttgat aaagttggtt
      301 ggccttctag acctcctcaa tcacggaatt ggaaagaaaa aagaaatggt tattggtact
      361 atggaaaatt gtatttacaa agttataaat cagctggaaa caattggatt gcgacttatt
      421 caggaacctt aactagaaag tagggatttt ttatgccaga atttgtgaac acaccaaatt
      481 tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc gaaagaagaa tcagctttac
      541 aagttcagcg aactttacag gagacacaaa aaattcaaaa atcaatttct aatctagaag
      601 attctgaaga aaaaagaaaa aaaagagacc aaaaaacata tcaaaaagaa aaataatgat
      661 tttaaaaaaa gagtgtctag tcgacattct ttttttctaa ttgaaagtgt tcataaaatc
      721 acccatttct ggacactttc tttttttgct ttatttttca aaaaaacaat tcatttcatg
      781 ttcataaacc cataacaata tctatgttca gttaaactat atggataaag ttatgttaca
      841 ataaaattaa tgaaggagtg atttttttga aaataggata cgcccgggta tctactggtt
      901 tacaaaattt ggatttacaa gaagacagcc taaataaatt tggttgtgaa aaaattttta
      961 ctgatcatat gagtggggca aaaagtaatc gaccaggatt agaaatggcc attgattttg
     1021 ttcgctctgg cgatacactt gtcgtttggc gcttagatcg tttaggtaga aatatggaag
     1081 atttgatttc aatcgtcaat cgactgaatg agcgtggtgt gagttttcat agtattcaag
     1141 aaaatattac gatggacaaa tcaagttcta caggacagct catgtttcat ttgtttgcgg
     1201 catttgcaga atttgagcgt aatttgatcc tggaacgatc cgcagcagga cgagcagctg
     1261 ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt gtctgaacaa gatttggaat
     1321 tactgaagac tttagtggat agcggtacac ctattaagac tattgcggaa cggtggaacg
     1381 tctcaagaac caccatttat cgttatttgg caaaaatgga ggagaaaaat ggaaagacaa
     1441 attctcatta gtttttgtca tttaatgctg aaaatttttg ccatttatct agaaaagtga
//
LOCUS       DAZUMV010000246         1439 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000246 DAZUMV010000000
VERSION     DAZUMV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.260"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            320..754
                     /locus_tag="ACG8FK_002886"
     CDS             320..754
                     /locus_tag="ACG8FK_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5098412.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            777..1097
                     /locus_tag="ACG8FK_002887"
     CDS             777..1097
                     /locus_tag="ACG8FK_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098413.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1238..1429
                     /locus_tag="ACG8FK_002888"
     CDS             1238..1429
                     /locus_tag="ACG8FK_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098414.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          562 a          210 c          306 g          361 t
ORIGIN      
        1 ttcgttaaaa agttttattt gttgtgttca acgaggaata ctttaacacg tgaaaatatt
       61 tcataggtaa aagaagcatg acaatttatg acaaatccac gttttttgta acagaatgat
      121 gttcgctcga tgtgaaaaat gatgaggaat ttcacataat gcagaaataa tgggattaga
      181 acaggtgttc tgatgagaaa agaaatgtat aaataacttg aaagaaattg cttttgttca
      241 tcattttatt tacatattgc gtatattttt tgtatttttg cttgagatgt tacaataaaa
      301 gaaaaaggag gcggttccaa tgagtaaaag ataccagaac atcatggtag cagttgacgg
      361 atcaagacaa tcgatcaaag cgttcgctga agcccttgac ttagcaaagg acaatgaagc
      421 ccacttattt atcgtgtcaa tcatcaacaa agttgagctg acccatagtg cctatgcctt
      481 ttcaaaaatc tatgcagatg aaaaacaaag aacagaagta gccatgctga aaaaaatcaa
      541 tgatgccaaa gagtacggca ttgctgagat ccacgcaata gttgaaactg gagatccaag
      601 aaatttgatt gccaatgtca tccctggaca agaaaatatt gatttgatcg ttatgggagc
      661 gacaggaaaa ggtgctatac aacaagcatt agttggatcg accgcttctt atgtcgtatc
      721 acatgcccct tgcagtgtgt tggtcgtgaa gtaatgagat aagggaggaa tagaaaatgg
      781 ttcgtcctca agaagtccgt gcgccaaaag aaaaaataga aattttagca attattgaag
      841 acggcacaca aacaaagaag ggatactcga tcgctttagt aaagtggaaa ggaaaaaaag
      901 gtgttgctct gagatgggat ggagataacc aacaagataa aggattccct gtcacagcta
      961 acggttatca tccagcgtgg tttgttctgc ctgataaatt tatggaatta tacagctatg
     1021 attatgcaag agccgcagca tcaatcaaag cattagataa attaacagaa gagcagaatg
     1081 aaatggagga aaaataaaaa agacgaacca atcagccttt agaaaactga ttagttcgag
     1141 gagtaaaaat gaaaaaaggt tatttgggat gaaactagaa taatgcgtat aacttaaata
     1201 aaacttaaag caataaaatg gcaagagggt gaggcaaatg gaagcaggaa aagtattaca
     1261 ggaagtaaaa gcactatgtg cagataaaaa atatgaagaa gcgaaaagcc tgatcgaaaa
     1321 gaacaaagaa aatttggaag aaaagtaccc gatcgcacaa gaatttatag aattaaaaca
     1381 aggaacgatc cttcaccgat tcaaacattt ttttggtata acagaataga aaaaacgat
//
LOCUS       DAZUMV010000247         1430 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000247 DAZUMV010000000
VERSION     DAZUMV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.261"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..104
                     /gene="ermCL"
                     /locus_tag="ACG8FK_002889"
     CDS             45..104
                     /gene="ermCL"
                     /locus_tag="ACG8FK_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HFS5098415.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            164..898
                     /locus_tag="ACG8FK_002890"
     CDS             164..898
                     /locus_tag="ACG8FK_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HFS5098416.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          536 a          216 c          204 g          474 t
ORIGIN      
        1 ttgtgctata attattctaa tttcataaaa aggagaatta atatatgggc atttttagta
       61 tttttgtaat caacacagtt cattatcaac caaacaaaaa ataagtggtt ataatggatt
      121 gttaatataa acattcatta taacctcata ggagtgggtt ataatgaaca aaaaaaatat
      181 aaaagatagt caaaacttta ttacttcaaa gcatcatata aatgaaattt tgagaaatgt
      241 acatttaaat acaaatgata atattattga gattggttca gggaaaggtc atttcacgtt
      301 tgaattagct aaaaggtgta attatgtaac cgccattgaa atagatccta aattatgtag
      361 gataactaaa aacaaactta ttgaatatga gaacttccag gttatcaata aagatatttt
      421 acaatttaag tttcctaaaa ataagtcata taagatatat ggaaatatac cctacaatat
      481 aagtacagat ataattcgaa aaattgtttt tgaaagcaca gctacagaaa gttatttaat
      541 agtggaatat ggatttgcta aaaggttgct aaatacaaat cgttcactag cactattttt
      601 aatgacagaa gttgatatat ccatattaag taaaatccct agagaatact ttcatccaaa
      661 acctagagtt aatagctcgt taattgtatt aaaaagacac ccttcaaaaa tatcactcaa
      721 agatagaaaa caatatgaaa attttgttat gaaatgggtt aacaaagaat acataaaact
      781 attttccaaa aaccaatttt atcaagcctt aaaatatgca agaattgacg atttaaacaa
      841 tattagcttt gaacaattct tgtctctttt caatagctat aaattattta atagataagt
      901 taaggggtgc ataaactgca tcccttaact tatcttattt tgcttaaaca atcttggccc
      961 gtaaactgca taactcatat gtaaatcgct cctttttagg tggcacaaat gtgaggcatt
     1021 ttcactattt ccggcaatta cttccatgta aagtataacc cactatactt tgcaagcaaa
     1081 gtgtgggctc gccgaggggt cttgcagacg gctctttcgg ctcttttgag ccgagtatca
     1141 gcgcaccctt atggtgcaat tagctgatac ctgtttgacg atttgctgcc ctaccttttc
     1201 ggtaggtatg caaacctcaa accaaaataa aaaccccctt cgacttcgtc agggggtttt
     1261 tatttatata aatctccgta aataatcgcg atttaataac atcttcaatt ttattttttt
     1321 gcgtatttta gaaatgtttt cactaaaaaa tatattatac aaaaaataca gtaatcgatt
     1381 ataattcttt gccagttaaa tatcatatcg ttattaaaat atcggaaaaa
//
LOCUS       DAZUMV010000248         1423 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000248 DAZUMV010000000
VERSION     DAZUMV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.262"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            679..765
                     /locus_tag="ACG8FK_002891"
     CDS             679..765
                     /locus_tag="ACG8FK_002891"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5098417.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1067..1291)
                     /locus_tag="ACG8FK_002892"
     CDS             complement(1067..1291)
                     /locus_tag="ACG8FK_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098418.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT          429 a          284 c          226 g          484 t
ORIGIN      
        1 ccgttttttc atccattcct ttcttccccc cttacaaata aataataacc tttaacaacc
       61 aactcgtaaa taggttggtt gttattatac aaaaggatat tagtactcag ataaaaagtt
      121 gttagtactc agataaaaag ttgttagtac tcagataaaa agttgttagt actcagataa
      181 aaagttgtta gtacccttct gtatccgttg ggagagtaag gctcaactgc cccctaaaaa
      241 gggttctaaa aaatctttaa aaaataaata aaataaacgc gcgcgcgcga gcaaaatttc
      301 ccgccaaaaa atcacccact ttttaaaata aacaggttcg cgcatgatat taaactgcca
      361 atctagctcc ctatggtcgc tgtaagaagg agtgatcgct acgctctcac attttacttg
      421 cagcagtcgc ctttaggctc cttcgccaaa aataattcat tagggactcc tttgtggcgt
      481 cctgagagct cgtctaagcc gttctaagac aagattcgat aaaaaactat gtccttcgtt
      541 ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac cccaaggcaa taaaaaactt
      601 aggcttattt ttgctttttt attgtataat tgattgtcgc ctatccgaca gtcttgcgac
      661 tggaaggagg tgtgtttcgt gttcactaac tttatttgct cccttcttgt tgggctttta
      721 agcgctttgt tcagttactg gttgaataac cgaaacaaga agtaaaactt aattaaggcg
      781 actcagccca caaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtgtttcg
      841 tgttcactta tttgcaatca aattataaca gaaataaata tttatttcaa aaatatccta
      901 caaaagtcta aaatccgtaa ttatttctta tatgcaacat ttacaaataa agtcgctctg
      961 agagctcgta tttttcgatt taagacgttt tatttagttt ttggtgtatt tcattggtcc
     1021 aagctaaaaa actcttagaa gcgattttag agccatttaa acccacttaa ttatattctc
     1081 catgaaattc gctccatgcg tcaaaagctg cgtctgaaat tccgccgtct tcgttcacta
     1141 gttgcattaa tttttccaac gatcgttttc tttcgggtgt catattttta tattccaaca
     1201 atttttcgtc tgttaaatcg aacttgattg cttgaattgc ttgctctaat ttttctaagt
     1261 tttgaaaata ttcgtaagga aatttttcca ttttttcttt ctccagtctc acaatttttc
     1321 tagaaataaa atcacttctt tgccgtctat actctcaaaa aaaatgtcgt ttgaaaaact
     1381 aaataaattt ttgatcgtgc atttttaaat ttggttatcg att
//
LOCUS       DAZUMV010000249         1417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000249 DAZUMV010000000
VERSION     DAZUMV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.263"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8FK_002893"
     CDS             complement(150..1337)
                     /locus_tag="ACG8FK_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS5098419.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZUMV010000250         1362 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000250 DAZUMV010000000
VERSION     DAZUMV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.264"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACG8FK_002894"
     CDS             82..>1362
                     /locus_tag="ACG8FK_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS5098420.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAZUMV010000251         1346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000251 DAZUMV010000000
VERSION     DAZUMV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.265"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(191..679)
                     /locus_tag="ACG8FK_002895"
     CDS             complement(191..679)
                     /locus_tag="ACG8FK_002895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5098421.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(701..1303)
                     /locus_tag="ACG8FK_002896"
     CDS             complement(701..1303)
                     /locus_tag="ACG8FK_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5098422.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT          422 a          313 c          195 g          416 t
ORIGIN      
        1 atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag tcttgcccga
       61 cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc tccaaaatca
      121 attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta ctttaacaca
      181 gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga aatccttgac
      241 cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa acctcgattg
      301 cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt tgcgcagcaa
      361 gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact ttatttagca
      421 aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg taaccgacag
      481 tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct ttttgaatct
      541 gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca atgatcggca
      601 caaaagcttc cggccatact tcttgtatga ctgccatcag ctcatccagt tcattttctg
      661 ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga ccttgcgctt
      721 gatggaataa aaagagcatg atcatgctat acacacccac cgaccatggt ttcaagaaaa
      781 tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact agacagatca
      841 agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta atatctttcg
      901 tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa ataagctgga
      961 caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca atcactaggc
     1021 caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc gctactgagg
     1081 agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga acaaatatca
     1141 tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc gatcgaaaaa
     1201 tcccatgatg ataagcataa agaatcaaca caaagaaaaa aaacagacca ataatgggca
     1261 tgatctgaag acatttttgt aaaactgatc cttttttaga catctatatt ccccacatct
     1321 tttttcattt attaaaactg cctcct
//
LOCUS       DAZUMV010000252         1329 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000252 DAZUMV010000000
VERSION     DAZUMV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.266"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..982
                     /gene="scrK"
                     /locus_tag="ACG8FK_002897"
     CDS             110..982
                     /gene="scrK"
                     /locus_tag="ACG8FK_002897"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5098423.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          410 a          234 c          309 g          376 t
ORIGIN      
        1 aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta aatatgatat
       61 atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta tgctatacgg
      121 tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag aaatgacgat
      181 caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt tagtcattga
      241 cttcttcaaa caataccaag cagatttagc agggattggg attggctcat ttgggcctat
      301 cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga aattggcatg
      361 gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga tttcttggac
      421 aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca aaggattgag
      481 cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac aagaagggcg
      541 ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa
      601 agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga tggccgctgg
      661 acccgccatc gaaaaccgct tagggaaaaa aggacaagag gttgcagaag atgatcctta
      721 ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct tgatgttttc
      781 tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt
      841 ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt taagcaaata
      901 tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg cattggctcg
      961 agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg ttggtagaga
     1021 aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact ggtttttgtt
     1081 ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta tggaaatttc
     1141 gatagccgta ggccacttgt attgtgcaat agttttttac aaaaaaaagg gggtgcggat
     1201 atttttttgg acctttttaa caaataggtt ctgttgcaaa gttttaaatc tactatcaga
     1261 taaggtagaa taatagaaaa agatagcagg aggaatgacg atgagtcatt ttaaaggaaa
     1321 gcaatttca
//
LOCUS       DAZUMV010000253         1283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000253 DAZUMV010000000
VERSION     DAZUMV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.267"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..1151)
                     /locus_tag="ACG8FK_002898"
     CDS             complement(12..1151)
                     /locus_tag="ACG8FK_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS5098424.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          334 a          281 c          239 g          429 t
ORIGIN      
        1 ttaaaaaaat cttacgctaa gtaggataaa ataattcctt gatatttttc gatcatatcc
       61 aaataattat cgatttcaac atactcatct acttgatgag gcaaagtaac gacaccaggc
      121 ccaaagacga caaagtcgaa ggaatggtta gctttcgtaa attcggctgc atccgttgtt
      181 gctgccgctc caactaaagg taatggctgg ctgaattgtt gctgaatgca atgaatcaaa
      241 ggagaatctg gatcagcttt gacaggtatt ttgttgtaat cgatcatcaa ttccaaatga
      301 tagtctgtct cttgattcaa ttcattgacg attgattgca gcaatgcaat gattttatca
      361 tttgggtatt ctggaatcga tcgaatgttc ccttggagct gcgcatggct tgggatgctg
      421 ttgacttgat tcccgccact gatcaatgta acattatgga ttgtttttcc taggacagga
      481 ttctcgattg tttcagcgag gtgattcatt tcagcatttg ctttcgtaat gaattcattt
      541 aggtgattga ttgcattata accttggtct ggcatagagc tatgcgcttc ttttccgtgc
      601 gaggtgactg tatagttgat tgatcccatg tgcgtataca tcaaactgta attggttggc
      661 tctccgatga tcaaggcatc taagtcatct acatagcctg ctttcgtgag ctgttcccca
      721 cctagttcac ccacttcttc tccgacagtg gctaataatt tgactgttcc gttaaatggt
      781 tttcctgact ctttcagttc gatcatggca atcaccattg ctgccaagcc actcttcata
      841 tctgtagcac cacgtccgta caaacggttc ccatggattt ctgctgcaaa gggtgcatag
      901 gtccaagacg attcatcacc agccgccacg acatccatat gcccagatag gcctaacact
      961 tttcctgatt ggcccttttg gtaacgagca atcaaattat ctctaccatc tctgtaagaa
     1021 acaatctctc ctgttatgtc atgctttgcc aaaagcttat tcaaataggc tgctacttca
     1081 ccttcgtttc cattcactga tttaattcgg ataatctctt gtaaaatcgc gattttttct
     1141 tcttttttca tctaatcctc tcccttgcca tttcttttta ttgtatttga gtgtgctcga
     1201 attttcaaat aggagcaacc aaagtaaaaa tgaagaaaga gagccacttt atgaaaaata
     1261 tgctgctact tccctcctat ttt
//
LOCUS       DAZUMV010000254         1266 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000254 DAZUMV010000000
VERSION     DAZUMV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.268"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1203)
                     /locus_tag="ACG8FK_002899"
     CDS             complement(31..1203)
                     /locus_tag="ACG8FK_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFS5098425.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          344 a          263 c          243 g          416 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacat
//
LOCUS       DAZUMV010000255         1247 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000255 DAZUMV010000000
VERSION     DAZUMV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.269"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8FK_002900"
     CDS             200..742
                     /locus_tag="ACG8FK_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098426.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1247
                     /locus_tag="ACG8FK_002901"
     CDS             898..>1247
                     /locus_tag="ACG8FK_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS5098427.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          515 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccaga
//
LOCUS       DAZUMV010000256         1195 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000256 DAZUMV010000000
VERSION     DAZUMV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.271"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8FK_002902"
     CDS             <1..1076
                     /locus_tag="ACG8FK_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5098428.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZUMV010000257         1160 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000257 DAZUMV010000000
VERSION     DAZUMV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.272"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..254)
                     /locus_tag="ACG8FK_002903"
                     /pseudo
     CDS             complement(1..254)
                     /locus_tag="ACG8FK_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(286..447)
                     /locus_tag="ACG8FK_002904"
     CDS             complement(286..447)
                     /locus_tag="ACG8FK_002904"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFS5098429.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            complement(771..1103)
                     /locus_tag="ACG8FK_002905"
     CDS             complement(771..1103)
                     /locus_tag="ACG8FK_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098430.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
BASE COUNT          332 a          245 c          150 g          433 t
ORIGIN      
        1 gtcccttaat tttgatatag gtttcgtcca ttttccatga atagaaggat tgtctatttt
       61 tcttcttcca aagataatcg aggactttgc tgtactcttg cacccaacga taaatcgtag
      121 tatgacaaac atttattcca cgatcatata acaattcctg aacttcacga tagcttagat
      181 tgtaacgcag gtagtaacta acagcgacaa taatgacgtc attttttgaa ttgtttgcct
      241 ttaaaatgat tcattactct atcctctctg tcttttttct caattttaca ctaaaataga
      301 ttttttggaa aactttgcaa cagaaccact tttcgtaata tcaatgtctt tattcgtatg
      361 atttttcgtt ttacgatcta catgattttg aagtccattt aacgacccaa ctttatgctt
      421 ttgtgttcgt gccaccatat aggccataac tgtgtacttc ctttcgttaa aattattgcg
      481 agaatcacaa gctaaattcg gaaaaacctc catgtaaggt ataacttact agacttatac
      541 tgcgtaaagt ctgtaagctc tgccgagggc tatatcggct taacagccga tgagaaactc
      601 cgtttctctc aaacgaaaaa gcgaacgaaa aacgactgcc ttcgcattcc ttttttacgt
      661 ccttttttcg ttcaaaacca ttggcttttt gcgtcattgt tttcacaatt tttattccgc
      721 aaaaaaccaa aagtgaaaag tattgctttt cacttcaacc cctcctcaat ttagttcaca
      781 ttttctgaac gaaattgtgc cataaattga gtcaaaccct taacatcatt cgtacgccaa
      841 tatgcaccat tttcttgcca aactaatcca tctgcaaatt ttcttagcga tactaaaacc
      901 tcatcattta ctgaatgatt tcttttattt gattccaaaa aatctaatgc ttcagcaggt
      961 gtcttaaagc cttccttttt acaatataat ttcgctaaag aaatcatttc tttctgtcta
     1021 atctgttttc gtttcgctct aatcttcgct tcttttgctt tcaaatccgc catttctttt
     1081 gccaatattt ctctattgtt cattttgatt cctcctaaaa ataaggcata tttctagttt
     1141 aaaatattta ttttacgaat
//
LOCUS       DAZUMV010000258         1148 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000258 DAZUMV010000000
VERSION     DAZUMV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.273"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..394
                     /locus_tag="ACG8FK_002906"
     CDS             41..394
                     /locus_tag="ACG8FK_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098431.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            complement(452..640)
                     /locus_tag="ACG8FK_002907"
     CDS             complement(452..640)
                     /locus_tag="ACG8FK_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS5098432.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(738..>1148)
                     /locus_tag="ACG8FK_002908"
     CDS             complement(738..>1148)
                     /locus_tag="ACG8FK_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS5098433.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
BASE COUNT          399 a          190 c          195 g          364 t
ORIGIN      
        1 ggttatatag gaacagtaaa atgtatagag gtgatttagt atgcgtaaaa aagaagataa
       61 atacgatttt agagccgttg gtttagcgat taaagaggct cgaatgaaac ggggtctcac
      121 tcgtgaacaa gtgggaacaa tgattgaaat tgacccacgt tatttaacaa atatagaaaa
      181 caaaggacaa cacccaagta cacaagtgct gtatgatctt gtatcattac tccatgtatc
      241 tattgatgaa tttttcttac ctacagataa tttgattaaa agcactcgaa ggttacaggt
      301 agaaaaatac atggatagct ttacagacaa agaactatcc ttaatggaag cactagcaaa
      361 aggtatcaat gaagcaagaa atattgaagg ctagtcagta aaattcagat aaacaaaaag
      421 agccgataag atgagagttt catttctcaa attatcggct ctgcgtcttg gcgtctggct
      481 ctttgattgt tattatattc attttttgaa cattaattaa agtttcgttc ttacatttgg
      541 ggcagtataa agggaaattt ttaagtatag tatccactcg tattcgtagt cttgttttat
      601 ttccacaaat aggacacaat atccacttgt agtttataat aacaatctcc tcctttccac
      661 tttaattcaa atctatatta aagaatattt catcttattt aataagaaac catatttata
      721 taacaacata aaacgcacta agttatttta ttgaacatat atcttacttt atctatccga
      781 ctatttagac gacgggtctg gcaaacaggt tcgccagtgg taacctgata tccttttagc
      841 tctgctaaac aaacactaag cccatttgta aaaaaagtta aatcattgcg ataatcttga
      901 atacatcgag caggaatttc tccaataata atgacctcat tatttttcag ttgagtattt
      961 acgatatttg cacaatattt gggagcatcg ttatatgccc gtgaaagata ttcctgtggt
     1021 gcataaactt taaaactaag atatggctct aacaattctg ttccagcttt tctaaaggct
     1081 tgctccagta caataggagt aagcatccga aaatctgctg gagtactaac agggctatag
     1141 tataaacc
//
LOCUS       DAZUMV010000259         1128 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000259 DAZUMV010000000
VERSION     DAZUMV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.274"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..>1128
                     /locus_tag="ACG8FK_002909"
     CDS             142..>1128
                     /locus_tag="ACG8FK_002909"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5098434.1"
                     /translation="MILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTPFCFIS
                     KQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCLLFDEF
                     RSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIVTDMNA
                     PYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYRQLKSL
                     FKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDLLTAYQ
                     EKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLH"
BASE COUNT          340 a          241 c          214 g          333 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaaatggca agaagatctc tatgattctc ctgaatcgta gcggtaataa acggacctat
      181 cttcggttga agaaacagcg atacctctgc cgagcatgta aaaaatattt taccgctcgg
      241 acgtatctcg ttaccccatt ctgctttatt tctaagcaga ttcattacaa gatcttagaa
      301 gaattgacag agcgtcaatc tataaaagca attggtaaac attgcgatgt ttctgtgaca
      361 actgttcagc ggacattgtc cgccttgtca tcagatatcc gtcagaatct tgagtattta
      421 cctcggtgtc tattattcga tgagtttcgt tctctttcta catggcacgg taaattttct
      481 ttttcttgta tggacggtga gaccgggaaa ctgtttgaga tcttacctag ccgccgtaaa
      541 aaagatctcg ttgctttttt catgcgtttt tctctcaaag cgaggttggg tgtacattac
      601 atcgtaacgg atatgaacgc tccttatttc tcactagtta aagagtgttt tccaaatgca
      661 caagtgatcg tcgatcggtt tcacattgtt caacatctga accgcttctt cgatgcggtt
      721 cgaaaacgtg tgatgaaaac acttgatcaa aaagacccaa tacaagctaa acagtatcgc
      781 caattgaaat ccttattcaa attactttta aaatcagagg atcgtttaga ttacaataca
      841 ctaacgaaaa gacgcaattt caattggaga ctattgactg aaacagaagt agttgaccgc
      901 cttctaaaga tttctcctga acttaaaaca gcttatcgtt actaccaaga cctgcttact
      961 gcgtatcagg aaaaagatcc agatactttt ttccagctgc ttcgagcaat gcctggagaa
     1021 gttccattgg aactgcacca tatcaaaaaa gcctttttca aatacgagaa agggattcgg
     1081 ctggcattac tgaagaagta ttcgaatgca cgactggaaa atctccat
//
LOCUS       DAZUMV010000260         1093 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000260 DAZUMV010000000
VERSION     DAZUMV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.277"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1070)
                     /locus_tag="ACG8FK_002910"
     CDS             complement(<1..1070)
                     /locus_tag="ACG8FK_002910"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5098435.1"
                     /translation="MAAKQTGLEFYIAGNWKYKSDEERKADEVKYGIKIYQVDFYRNP
                     LNLKNIKAYHQIVEIIKNEKIDIIHCNTPIGGVVGRIAGLKCKVKKVIYQAHGFHFYS
                     GAPKINWFIYYPMERWLAHYTDVLITINHEDYTLAQKLRLRKGGKAYYIPGVGIDTNR
                     FMSSTSIRNFRESICKELNIPDTAKIILSVGELNENKNHLSVIHALQKIPLHTDIYYI
                     IAGVGEMRETLQNIANELGLHQRVFLLGYRSDIPKLLAAANIYILPSLREGLNASLME
                     AMASGLPCIVGNIRGNVDLIEDGKGGFLINPKDEEEIAGAINRLADNKLLCESMGAHN
                     LDKIKDFDLSVVINQINNVYHE"
BASE COUNT          336 a          206 c          138 g          413 t
ORIGIN      
        1 atttcatggt aaacattatt tatttggttt atcacaactg ataaatcaaa atcttttatc
       61 ttgtctaaat tatgtgcccc catagattca cacaataatt tgttatcagc caatctattt
      121 atcgcaccag caatttcttc ttcgtcttta ggattgatta gaaatccacc cttaccatct
      181 tctataagat ctacatttcc tcttatatta ccgacaatac atggtaaacc actagccatc
      241 gcttccatta aagaggcgtt gaggccttcc cttaatgagg gtagaatata tatgtttgca
      301 gcagctaata atttgggtat atcacttcta tatcctagta aaaaaactcg ctgatgtaat
      361 cctaattcat tagcaatatt ttgtagtgtc tccctcattt ctcctacccc agcgataata
      421 taatagatat ctgtatgcag cggaattttt tgtaatgcat gtattactga taaatggttt
      481 ttattctcat ttagctctcc tacagataaa atgatttttg ctgtatcagg tatattaagt
      541 tctttacata tactttctct aaaattcctt attgatgtgg aagacataaa cctatttgta
      601 tctataccca cacctggaat ataataagct tttccacctt tcctaagacg aagtttttga
      661 gctaaagtat aatcctcatg atttattgta atcaaaacat ctgtataatg agctagccaa
      721 cgttccatag gataatatat aaaccaattt attttaggag cacctgaata aaaatgaaat
      781 ccatgtgcct ggtaaattac ttttttgact ttacatttaa gaccagcaat ccgtccgaca
      841 acacctccaa taggtgtatt acaatggata atatctattt tttcattttt tataatctct
      901 acaatctgat gataagcttt tatatttttt aaatttagtg ggtttctata aaaatcaact
      961 tggtatattt ttattccata tttcacttca tcagcttttc tttcctcatc ggatttgtat
     1021 ttccagttac cagctatata aaattctaaa cccgtttgtt tagctgccat catactagct
     1081 ttagaaaagt tat
//
LOCUS       DAZUMV010000261         1091 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000261 DAZUMV010000000
VERSION     DAZUMV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.278"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..979
                     /gene="bsh"
                     /locus_tag="ACG8FK_002911"
     CDS             5..979
                     /gene="bsh"
                     /locus_tag="ACG8FK_002911"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS5098436.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQRIKYIN"
BASE COUNT          382 a          136 c          188 g          385 t
ORIGIN      
        1 aactatgtgt acgtctatta cttatgtaac aagtgatcat tattttggaa ggaattttga
       61 ttatgaaata tcttacaatg aagtagttac tgttactcca agaaattata agttgaattt
      121 tcgaaaggta aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc
      181 tgactaccct ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa
      241 tttttctggg tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt
      301 tgaatttatt ccttggattt taggacaatg ctcaacagta ggagaaacta aaaaattgtt
      361 aaaaaatatc aatttagcaa atataaatta tagtgatgaa cttcctttat cccctttaca
      421 ttggctatta gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca
      481 tatatatgat aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt
      541 taatttaaac aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca
      601 aataagtttg aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc
      661 ctcagtatct cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc
      721 agagtcagaa agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg
      781 tttgtgtgat gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt
      841 tgacaaagga atctattatt atcgaacata tgaagacagt caaattactg caattgatat
      901 gaataaagaa gacttagata gtcataagtt aattagttat ccaattatag aaaaacaacg
      961 aattaaatat ataaattagt taatgtgttg tgattgatta tttaatatag atataataca
     1021 aaaaggcaaa gatattttca aaacattttt tcttgatgtg atatccttgt cttttttgta
     1081 atgtttttaa c
//
LOCUS       DAZUMV010000262         1070 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000262 DAZUMV010000000
VERSION     DAZUMV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.279"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1070
                     /locus_tag="ACG8FK_002912"
     CDS             <1..>1070
                     /locus_tag="ACG8FK_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5098437.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYHGSAKSNIGIEQLIEVITNKFY
                     SSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTS
                     INGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKP
                     QQREMLLDALLEISDSDPLLQY"
BASE COUNT          386 a          169 c          223 g          292 t
ORIGIN      
        1 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
       61 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      121 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      181 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      241 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      301 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      361 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
      421 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
      481 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
      541 caagaggaaa gcataagatt tcagaattgc tccttgtacc ctgtttatca tggaagcgca
      601 aaaagcaaca tagggattga gcagcttata gaagtgataa cgaataaatt ttattcatca
      661 acatacagaa agaagtctga actttgcgga aatgtcttca aaattgaata ttcggaagaa
      721 agacaacgtc ttgcatatgt acgcctttat ggcggaatcc tgcatttgcg ggattcggtt
      781 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
      841 ttatgtaaaa ttgataaggc ttattccggg gaaattgtta ttttgcaaaa tgagtttttg
      901 aagctaaata gtgttcttgg agatacaaag ctattgccac agagagagag aattgaaaat
      961 ccgctccctc tgctgcaaac aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta
     1021 cttgatgcac ttttagaaat ctccgacagt gacccgcttc tacaatatta
//
LOCUS       DAZUMV010000263         1062 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000263 DAZUMV010000000
VERSION     DAZUMV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.280"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>1062)
                     /locus_tag="ACG8FK_002913"
     CDS             complement(37..>1062)
                     /locus_tag="ACG8FK_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS5098438.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAZUMV010000264         1010 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000264 DAZUMV010000000
VERSION     DAZUMV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.282"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(272..886)
                     /locus_tag="ACG8FK_002914"
     CDS             complement(272..886)
                     /locus_tag="ACG8FK_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5098439.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          322 a          205 c          157 g          326 t
ORIGIN      
        1 tggattcttt cttgtctctt tttccactac tttttatttt tctgacacta catattctta
       61 taactcattt ctcataactg tgaaacaact tataaaaata acaaacttta aacaaaacaa
      121 gcttaaaatc aaagatgatc catcttaaaa taactaaaac aaaaaaagta gtcgttgtta
      181 atcctcatta ataatcgttt tctttttttc aaacgctcca tttttatgtg gaacattatt
      241 tttttaagga actttgtcaa catgtggata actattttcc acttgtgtcc tggactagct
      301 gttttgctgt aaaatacaaa acttgctgca gcaaaaagat cgttgcaaga gaggctgttc
      361 cgcagatcgc tggatctaga tttggcaaag tcaagactgc atcatgtaaa ttggcgtggt
      421 tgtccaaaac caaatcagca aattcaaata atcgcaaacc actgctatgt tgcgatttga
      481 tacttcgtga caaatcaaat ccagtaacaa tgattaatgg atgaccattt tctttgatcc
      541 actgagctag ttcgatgatt gccgggtttc ttccttcatt cgaaagaaga agaaaaatgt
      601 cttctgattt gacagaaagc tgatcaataa ttgcctgtcc tgcaccttct agcgtttcat
      661 agacacctcc ggctggattt tcaatcaata tacttggtac aagatccggg gtttgatccc
      721 aaaattcaaa tgctacacca cttagtgtgc ggctagcata tatctgcaat ctaccacctt
      781 gattaatttg tttcgcgatc caagtgccag cttgaagcat cagcggctct tctatcctta
      841 atttctcttc caattggtga taatagtcaa aataacataa actcatttat ctctcccgcc
      901 tagctatact tgacatgaat agaaacagct gcctaatcta aggaagtaca taaaaaaaca
      961 gtagttagac agtaacacat gtggtctttg ctgtttcaaa cacaaaaaag
//
LOCUS       DAZUMV010000265         1002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000265 DAZUMV010000000
VERSION     DAZUMV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.283"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..636)
                     /locus_tag="ACG8FK_002915"
     CDS             complement(82..636)
                     /locus_tag="ACG8FK_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5098440.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          325 a          186 c          107 g          384 t
ORIGIN      
        1 acttccgaaa attcagttat cttcattaat ctcaccttct aagttttcat tatacacaaa
       61 aaatatatcc taatatctca attagcctcc caatttaaaa ccacgtaacg atgcccctat
      121 ttcatcttca gttataccaa tgtacctttt tgtaattgct tgcgagctat gattaaaaat
      181 aaacattaag gtagcaatgt ctctagtttt cttgtagtag tgatacccaa aagtttttct
      241 taatgtatgt gttcctatat catctcttcc taatttttct gctacatctg catatatctg
      301 atagacacga tcccttgtta tatgtcgacc ttgttgccga cttggaaata accaatcatt
      361 ttcttcttta ccttcagtat ataataaaat ctcttctctc aaattatcta taaaaatcgg
      421 ttttcgctta cctgttttat gttcaacaat aataggctta cttttacctc gtatgtctcc
      481 aacttttaat cctataatat ctgacatacg taacccatta ttgattccaa acaaaaataa
      541 aaaactatct ctttctggat tacttgttct tcttagccaa aactgaaaat cttgtatctc
      601 ttcatttgtc cttaacggtt gtacattata gctcactatt gtttcccttc ttaaaaatat
      661 tctgagttca tgattatata tatattttac cataatcatg aacttatata tatactctgt
      721 taaaagtgaa gaaaatcaag gggtttagtt tatgatttta tatcatttca gtaactcaga
      781 atattccgac ttttttgtac aaaaaaatta accttactta tatgaagatt ctaagctctt
      841 atttaaattt tagacgggca gttatccgta gaagttctta gaatagctaa tttacataat
      901 taaattatat gaaatttaag tttgaacaat atactaagaa ctccccttac tataaagtga
      961 aataaattag catatgaata accgtgctac caaacttttc at
//
LOCUS       DAZUMV010000266          999 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000266 DAZUMV010000000
VERSION     DAZUMV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.284"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..>999
                     /locus_tag="ACG8FK_002916"
     CDS             49..>999
                     /locus_tag="ACG8FK_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5098441.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSS"
BASE COUNT          394 a          127 c          171 g          307 t
ORIGIN      
        1 tgatacattt gtaaaaataa aaaagatgat ttttaggaag tgaagagagt gcataaaatt
       61 agtgttatag tacccgcata taacgtagaa gactatttag aaaactgctt agaatctatt
      121 tccaaccaga cacattcaaa tattgaaata atagtagtca atgatggttc cccggatcga
      181 agtggggaaa ttgcagaaga atataaaaag aaagatatgc gattaaaagt gatacatcaa
      241 gtgaatggtg gtttaagtgc ggctaggaat tctggtcttg attctgcaac aggagatttt
      301 gtgtgcttta ttgacagcga tgattggata gaaccagact atcttgagtt actatatttt
      361 ggcacgcaaa cttatggagc agatatctcg gtgctaaaaa taaaaaagat ttcaagttta
      421 gaacaactaa aagaacatac aattgctgag agaaaatggg aaatatttga tagagaacaa
      481 gcaatgaaaa acttatttat aaataataaa attggctatt ctgcaaataa taaattattc
      541 aatagaaatt tatttgataa tataagattc ccactgggta aattaatgga agataaggct
      601 actacttatt tgctgatcga tcattccgaa aaagttgctg tcaattcatc tgctaaatat
      661 cactattacc aaagccctaa aagtatactg agaggaagct tcaatcctag aatttttgat
      721 tcttttgaag tccatgaaga tatactaata ttcatagata agttttatcc ggaattatca
      781 attaatgtta gaactcgcta tgtctatgaa gcaataagga tgatgatgac aatgattaaa
      841 aataactata ccaataaaga agattattta cgttctataa atattttaca acgctttgaa
      901 aaagaagtga aaattgatcc cgacttttca ccaaaattaa aaaaattagt aaagttttta
      961 ttaataaata aaaacttgcc ttactatatg tcaagtagt
//
LOCUS       DAZUMV010000267          968 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000267 DAZUMV010000000
VERSION     DAZUMV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.285"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..746
                     /locus_tag="ACG8FK_002917"
     CDS             <1..746
                     /locus_tag="ACG8FK_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS5098442.1"
                     /translation="ENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRN
                     LENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQ
                     ENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEI
                     ANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSS
                     HFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            763..>968
                     /locus_tag="ACG8FK_002918"
     CDS             763..>968
                     /locus_tag="ACG8FK_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098443.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESIS"
BASE COUNT          363 a          110 c          172 g          323 t
ORIGIN      
        1 cagaaaatct aagtaaaact atcgagcaaa tcaaaataga taatctgtta tcacaaaatt
       61 acgatccaga aatatatctg tttctttatg aaaataaaat actggaatat gttaaaaatg
      121 gagatattcg aaatttagaa aacatggttt ttaatctgag taatggaata attccaagtg
      181 ttagtggaga tacgattcgg tctgaaaaaa attattctat cattgttttt gaaaagttag
      241 cacaaacgtc tataacatta ggaatggata taatagaagc gtatcaaagt agagatgctt
      301 tgatacaaga aaacgaatta gctgtatcgt tacctgaggt cttaaaagtg agggattctg
      361 ggattgttta ttatactaag gaaatcggaa aaactaagat agagcatctt tctcccttaa
      421 tttcttctgt cgttcaattc atcggcttaa atatctataa aagaattact gtaaaggaaa
      481 tagctaatta tttttctgta agtgaaacta aattaagaga aagctttaag aatgaaatgc
      541 atattacgat ttataattat atctctaagc gaaaaatttc tacggctaaa ataatgttga
      601 aatcaaacca tactatcagt gaagtttctt taggtttagg attttcagat tcttcacact
      661 tttcgagagt attcaaaaag tacgcaggtg tatctcctaa gcaatatcaa ttaggtttag
      721 tggacaattt caacaacatt agctgagagg ttttatttta ttatgagact atatcattta
      781 cgtagtttgg aggagttgag gaaattggat tttaacaaaa aatttttttg ggaaatttct
      841 gatttgagtt gtttcgtaga atctgagcgt ctaagtaatg gtaataatag tgtgttaaat
      901 gaatatgaca aagtagtaat taataaagta aaaagtttat gtgaagaagc tagagaatct
      961 atcagtca
//
LOCUS       DAZUMV010000268          943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000268 DAZUMV010000000
VERSION     DAZUMV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.286"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8FK_002919"
     rRNA            <1..>943
                     /locus_tag="ACG8FK_002919"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZUMV010000269          897 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000269 DAZUMV010000000
VERSION     DAZUMV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.288"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..879
                     /locus_tag="ACG8FK_002920"
     CDS             256..879
                     /locus_tag="ACG8FK_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5098444.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          331 a          129 c          167 g          270 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
      781 gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg agttacaaga
      841 aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt tagaaag
//
LOCUS       DAZUMV010000270          897 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000270 DAZUMV010000000
VERSION     DAZUMV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.289"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>897
                     /locus_tag="ACG8FK_002921"
     CDS             <1..>897
                     /locus_tag="ACG8FK_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS5098445.1"
                     /translation="INTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGEL
                     TLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTP
                     QTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDG
                     TKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQ
                     HCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQ
                     SLIENQDLLTFYDFPPSIRRTIY"
BASE COUNT          322 a          170 c          153 g          252 t
ORIGIN      
        1 ataaacacac tcctacggac tgaattaacg gcttttttag attacgaaaa atatgatcgc
       61 actggtttta attcaggtaa ttcgagaaac ggttcttact ttcgatcaat caaaaccgaa
      121 tatggtgaat taacattgga aatacctaga gatcgtaatg gtgagtttaa acaacaaact
      181 ttaccagcct acaaaagaac aaacgataca ttggaaacca ctattatcca tttattcgaa
      241 aaaggtgtta cgatgtctga aattgctgat ttgatcgaaa aaatgtacgg tcatcactat
      301 actccacaaa ccatgtccaa catgactaaa gttctgactg aagaagtaaa tgcctttaaa
      361 tccagagcct taaatgataa gtatgtcgct atttttatgg acgctactta cattccacta
      421 aaacgtcaaa ccgtatccaa agaagcgatt tatattgcca ttggtatacg agaagacggc
      481 actaaagaag tactgagtta tgcgattgct ccaactgaat caacatacgt ttggaatgag
      541 ctgctacagg atattaactc cagaggagtt caagaagtct tgctttttat tacggacggc
      601 ttaaaaggca tgaaagatac tatccatcaa atttatccta aagcaaaata tcagcattgt
      661 tgtatccatg tatctcgtaa catcgctcat aaagtacgtg tcaaagaccg aaaagaaatc
      721 tgtgatgact ttaaggctgt ttatcaagct aactcaaaag aagaagcgaa taccttctta
      781 tccggcatga ttgagaaatg gaagaaaaac tatcctaaag tgacgcagtc actcatagaa
      841 aaccaagact tattaacttt ttatgatttt ccacctagca ttcgtagaac catttac
//
LOCUS       DAZUMV010000271          890 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000271 DAZUMV010000000
VERSION     DAZUMV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.290"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG8FK_002922"
     CDS             <1..888
                     /locus_tag="ACG8FK_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS5098446.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAZUMV010000272          882 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000272 DAZUMV010000000
VERSION     DAZUMV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.291"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8FK_002923"
     CDS             168..362
                     /locus_tag="ACG8FK_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098447.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..>882
                     /locus_tag="ACG8FK_002924"
     CDS             631..>882
                     /locus_tag="ACG8FK_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098448.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSN"
BASE COUNT          365 a          109 c          139 g          269 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga at
//
LOCUS       DAZUMV010000273          881 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000273 DAZUMV010000000
VERSION     DAZUMV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.292"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..159
                     /locus_tag="ACG8FK_002925"
     CDS             <1..159
                     /locus_tag="ACG8FK_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5098449.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="ACG8FK_002926"
     CDS             242..859
                     /locus_tag="ACG8FK_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098450.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
BASE COUNT          308 a          109 c          172 g          292 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa a
//
LOCUS       DAZUMV010000274          871 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000274 DAZUMV010000000
VERSION     DAZUMV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.293"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..309
                     /locus_tag="ACG8FK_002927"
     CDS             136..309
                     /locus_tag="ACG8FK_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098451.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          290 a          143 c          106 g          332 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt g
//
LOCUS       DAZUMV010000275          870 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000275 DAZUMV010000000
VERSION     DAZUMV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.294"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..129)
                     /locus_tag="ACG8FK_002928"
     tRNA            complement(57..129)
                     /locus_tag="ACG8FK_002928"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:His,seq:gtg)
     gene            complement(132..205)
                     /locus_tag="ACG8FK_002929"
     tRNA            complement(132..205)
                     /locus_tag="ACG8FK_002929"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Trp,seq:cca)
     gene            complement(212..292)
                     /locus_tag="ACG8FK_002930"
     tRNA            complement(212..292)
                     /locus_tag="ACG8FK_002930"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Tyr,seq:gta)
     gene            complement(294..369)
                     /locus_tag="ACG8FK_002931"
     tRNA            complement(294..369)
                     /locus_tag="ACG8FK_002931"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Phe,seq:gaa)
     gene            complement(405..477)
                     /locus_tag="ACG8FK_002932"
     tRNA            complement(405..477)
                     /locus_tag="ACG8FK_002932"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(442..444),aa:Asp,seq:gtc)
     gene            complement(487..559)
                     /locus_tag="ACG8FK_002933"
     tRNA            complement(487..559)
                     /locus_tag="ACG8FK_002933"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(524..526),aa:Val,seq:tac)
     gene            complement(575..646)
                     /locus_tag="ACG8FK_002934"
     tRNA            complement(575..646)
                     /locus_tag="ACG8FK_002934"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(611..613),aa:Glu,seq:ttc)
     gene            complement(661..750)
                     /locus_tag="ACG8FK_002935"
     tRNA            complement(661..750)
                     /locus_tag="ACG8FK_002935"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ser,seq:gga)
     gene            complement(790..863)
                     /locus_tag="ACG8FK_002936"
     tRNA            complement(790..863)
                     /locus_tag="ACG8FK_002936"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(827..829),aa:Asn,seq:gtt)
BASE COUNT          243 a          238 c          199 g          190 t
ORIGIN      
        1 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaaaagggcg
       61 aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc
      121 cacattcgcc atggcagggg cagcaggaat tgaacccaca ctaacggttt tggagaccgc
      181 tgttctacct ttaaactatg cccctataaa atggaggaga gtggattcga accactgaac
      241 cctaaggaac ggatttacag tccgtcgcgt ttagccactt cgctactcct ccatggtggc
      301 gcgggacaga atcgaactgc cgacacacgg agcttcaatc cgttgctcta ccaactgagc
      361 taccgcgcca tattttaaaa ttattaattg aataacaaga acgacggttc cgacgggaat
      421 cgaacccgcg atctcctgcg tgacaggcag gcatgttaac ccctacacca cggaaccagt
      481 tcattatgga ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc agatgctctc
      541 ccagctgagc taaacctcca acattcaatt aaaatgaccc gtacgggatt cgaacccgtg
      601 ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa cgggccaggt aaatttgcta
      661 cggagagtaa gggattcgaa cccttgagac agtttggcac catctacatg atttccaatc
      721 atgctccttc ggcctctcgg acaactctcc agagtgaaag aagctcaata ctctttctca
      781 aaatgagaac tccggcagta ggactcgaac ctacgacatc atgattaaca gtcatgcgct
      841 actaccaact gagctatgcc ggaataacat
//
LOCUS       DAZUMV010000276          824 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000276 DAZUMV010000000
VERSION     DAZUMV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.295"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..739
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8FK_002937"
     CDS             <1..739
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8FK_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFS5098452.1"
                     /translation="KGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIK
                     GTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLR
                     ETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDF
                     GDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIET
                     IVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          364 a           54 c          134 g          272 t
ORIGIN      
        1 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
       61 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
      121 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
      181 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
      241 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
      301 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
      361 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
      421 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
      481 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
      541 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
      601 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
      661 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
      721 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
      781 taaatagttt aaaaaaatga aaaataataa aggaagtgag tcaa
//
LOCUS       DAZUMV010000277          815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000277 DAZUMV010000000
VERSION     DAZUMV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.296"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(504..728)
                     /locus_tag="ACG8FK_002938"
     CDS             complement(504..728)
                     /locus_tag="ACG8FK_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098453.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          291 a          114 c          143 g          267 t
ORIGIN      
        1 ctctatttac ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa
       61 aaaagtgggt tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa
      121 tatttattcg tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat
      181 ggtttggcgc agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca
      241 aaagctactt gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg
      301 tttctacctt gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa
      361 aaaggatggt gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc
      421 attatgccaa taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac
      481 aggaaagttg aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc
      541 aagataaaga tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa
      601 cgagtttgtt ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa
      661 gatccgatga tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc
      721 gatttcattg aatagtcata gcctccttat tctttcatat gttgaaattc cagtataaaa
      781 actggaagga actgtacgtt ttttagaata aaaat
//
LOCUS       DAZUMV010000278          812 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000278 DAZUMV010000000
VERSION     DAZUMV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.297"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..381
                     /locus_tag="ACG8FK_002939"
     CDS             97..381
                     /locus_tag="ACG8FK_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098454.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            384..800
                     /locus_tag="ACG8FK_002940"
     CDS             384..800
                     /locus_tag="ACG8FK_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098455.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          332 a          139 c          120 g          221 t
ORIGIN      
        1 tagcttatgt atagcatttt ggcaaaaaac agctatacaa atatagtatt tgtatggtat
       61 acataaatta tacataattg caaaaggaga ttttttatgg acattttagt tcggaatatt
      121 gatccaaagt ttgttgcaac aatagacaaa cgttgccaag aactcacgaa taaaacagga
      181 gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg aaagtgattt tgaccgtcaa
      241 cttacgactt acaaaaaaga ccaattcgat ttattgctcg ataaatttat tgctatccaa
      301 acagaaaata ctcaaatatt aagcgagtat gtctacacaa ataatcaaat cattgaaatg
      361 cttatcggag gaatgatata accatgagaa aaacttctgt ccaaaaacat gttactttgt
      421 accctagtca tgttgacgtt ttagattcca tcatcgaaaa aaatccagat atcaataact
      481 actcggaagc cattcgcttt ttatgtatga gttatgaacc cgataccaac acgaccacta
      541 ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat ttccatcatg actgaaatca
      601 tcgcagaatt tgctgattta tatctaaaag atacaggcgt tcttattggc acaaattcta
      661 aagtatatca agaagtgaaa gaaaaaatcg aagctaaact aaatccatca aatcgaaatc
      721 gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac ttctagaaat ttcgacccaa
      781 caaattttct aaaacgataa aaaacagata gg
//
LOCUS       DAZUMV010000279          808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000279 DAZUMV010000000
VERSION     DAZUMV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.298"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..693
                     /locus_tag="ACG8FK_002941"
     CDS             <1..693
                     /locus_tag="ACG8FK_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5098456.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          296 a          128 c          148 g          236 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct cccacttc
//
LOCUS       DAZUMV010000280          783 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000280 DAZUMV010000000
VERSION     DAZUMV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.299"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>783
                     /locus_tag="ACG8FK_002942"
     CDS             <1..>783
                     /locus_tag="ACG8FK_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5098457.1"
                     /translation="ALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGL
                     AKLKDAKVVSGEQRINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEEN
                     DKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGL
                     SSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESK
                     YFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLIL"
BASE COUNT          338 a           64 c          131 g          250 t
ORIGIN      
        1 gctttatatg aaagttctaa ttcttcaata tcaataacaa aaaaagatgg taaatatttt
       61 aatgttaatc aatttaaaga tattgaaaaa ttaaaagaaa ttgaagaaaa aataattcaa
      121 tatgatggat tagcaaaact aaaagatgct aaagtagtta gcggagaaca aagaataaat
      181 agagaagatt tatctgacga atttaagaat gttgtttcac tcgaagctac aaataatact
      241 aaaagaaata ttttatttag tagtggagta tttacaatta aagaaggtaa aaatatagaa
      301 gaaaatgata agaattcaat tattgttcat gaagaatttg ctaaacaaaa caatctaaaa
      361 ttaggtgatg aagttgatct tgaattacta gatattgaaa aaagtggaaa aataaaaagt
      421 cataaattta aaattatagg gatcttttct ggtaaaaaac aggaaacata tacaggatta
      481 tcttctgatt ttagtgaaaa tatggtgttt gtagattatt caacaagcca agagatatta
      541 aacaattcag aaaataatga aattgcaaat aaaattttaa tgtattctgg tagtgcagaa
      601 tctacagact tagccttaaa caaattgaaa gagcttaaaa ttgatgagtc aaagtatttt
      661 gttgaaaaag attctaatgc gtttgaagag tctttagagt cagtgagtgg aataaaatat
      721 ataataaaag taatgactta ttctattatg ttgggtggga tggttgttct ttcattaatc
      781 ttg
//
LOCUS       DAZUMV010000281          753 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000281 DAZUMV010000000
VERSION     DAZUMV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.302"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..>753
                     /locus_tag="ACG8FK_002943"
     CDS             171..>753
                     /locus_tag="ACG8FK_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFS5098458.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEA"
BASE COUNT          264 a          130 c          131 g          228 t
ORIGIN      
        1 agtctaactt ttggggtgca catcactttg aggggcggta cataaaaatt tattatttaa
       61 cacgtggtgc tagttaattt tttcctagag gaaaatgtct aaacatgttg gtaaatcaac
      121 aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg aggactacat atgcgttcat
      181 caaaatatac tgaacattat acagatactt ttataacatt caaggaaatt atgagaactg
      241 tttctaacat ataccacaat tgtgtaccag ataagattaa aaaccgaaga aatactgatc
      301 aactgaagca acgcgataca gtgattattg cttgtgttat atggggcata attaatggct
      361 atactagcca aagagccacg tatagagcgg tttgttccgt cttatttcct aatggtgact
      421 ttcctagtag gagtcgattc actcgcttaa gttcaaactt agcttacact atcaagatta
      481 ttcgatactt tttcatcaag aagctcacca aaggtgaact agtcggaatt atagatagtt
      541 ttcctagtcc attatgtaaa ccagttagaa atagacaagc aaaactattg aatcaaatag
      601 ccaaagttgg ctataactca acaaaaaaat cttattttta tggtcttaaa attcatatga
      661 tcgttactaa gactggcttt ccgattactt actcaatcac gaatccaggt gtccatgacg
      721 tgaaagtgtt agagacttta tcggaagaag cga
//
LOCUS       DAZUMV010000282          729 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000282 DAZUMV010000000
VERSION     DAZUMV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.303"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..703
                     /locus_tag="ACG8FK_002944"
     CDS             <1..703
                     /locus_tag="ACG8FK_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5098459.1"
                     /translation="RRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQ
                     LIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNY
                     EKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRD
                     ISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNF
                     KLRIMIRHGKTFLTK"
BASE COUNT          235 a          147 c          136 g          211 t
ORIGIN      
        1 tcgacgcttg ccttatttag aacgctattt ttctcggttt tcattagcga ctcgtgaagc
       61 cgttcaattg attgttattg atatgtatgc gccttatgtt tcgttggtga aaaagctttt
      121 cccgaatgct cagttgatca tcgatcgttt ccacatcgtc caacatattg gcagaacttt
      181 cttgaatcat cgagtgaaag aaaccacggt acgacttaaa ggaaattccc actctcaacg
      241 agggttaggg aaaaaattga agcgctattg gaaactccta caaaaagaag aagcaaagtt
      301 aaactatgaa aaacgggttt ggcgtgccag ttttaaagat tatttgactg aaagtgagat
      361 tgccgatcga ctgttagctg cttgtcccaa cttacgccaa ggctatcagt tataccaaga
      421 cattctttat gcggtaaaga aaagagatca gtcactattt gaggattgtt taactagaga
      481 cattagcggt ctgccagaaa cctatacaac cactttacga acgtttaaaa agtttctgcc
      541 acagatccaa aatgcgcttc actacagcta ttcaaacgga ccattggaat gcttaaacaa
      601 ccacatcaaa gtattaaaac gcaatgcgta cggctttcgc aacttttaca atttcaaact
      661 acgaattatg attcgtcatg gcaaaacatt tttaaccaaa taaacagaac agaaagaggt
      721 tttcttctt
//
LOCUS       DAZUMV010000283          708 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000283 DAZUMV010000000
VERSION     DAZUMV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.304"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            524..>708
                     /locus_tag="ACG8FK_002945"
     CDS             524..>708
                     /locus_tag="ACG8FK_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5098460.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINV"
BASE COUNT          263 a          107 c           97 g          241 t
ORIGIN      
        1 tttcatcatg atgaatacta tttacatata tctagttcaa agacaataat tattatgatc
       61 cgttattatc taagcactat acagataata ggaatttttc gtttctaaat attccaattt
      121 gggaatccaa taaaaatttt cgttcaaata cgtgtctatt cgaactcttt tctagttgtt
      181 aattatgaag ggggaagaaa taaaaaatac aatctataca cgaataagaa tattatccaa
      241 aaggaggttt tagaaaagat ttattatacg taaaaagctc aagacatttt cttagccata
      301 aggacatgtt tgatatttaa gaagatatat gaattttctt ttctaagtat aaagataact
      361 atccattatc aactaaaaaa acatttttga aaataaacat cagcagtcct atcaatgcat
      421 gtgagcttat agaagttatt cgcagcgcag cataattgca ttagcttctt ttcgtgtttt
      481 ttgcctcttt tccctttaaa tcttaatgga ggaggtgtgc aatatgtcag ataacgacga
      541 acgcgtaaat gcccagttga ttcgattctt tgaaaaggtc attttgaaca ccgctaatac
      601 ttattataaa aaacaagaag aaattcaaaa acatgaacaa cttgatcaac tcccaccata
      661 tttgtttact aaagatacta taaaatttaa acaaccgatt aatgtttt
//
LOCUS       DAZUMV010000284          696 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000284 DAZUMV010000000
VERSION     DAZUMV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.305"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACG8FK_002946"
     CDS             complement(54..629)
                     /locus_tag="ACG8FK_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS5098461.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAZUMV010000285          679 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000285 DAZUMV010000000
VERSION     DAZUMV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.306"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..461)
                     /locus_tag="ACG8FK_002947"
                     /pseudo
     CDS             complement(1..461)
                     /locus_tag="ACG8FK_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
BASE COUNT          226 a          150 c          108 g          195 t
ORIGIN      
        1 aaatacctaa tgacatcaag atcggcaaca caataactgc aatagaaatc actggtccga
       61 ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc
      121 ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat
      181 ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt
      241 actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca
      301 tcgccgagtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca
      361 tgatgacaaa agccgaccat caagttgtag ccttttacac agtaccagac catgaaaaag
      421 aatgagataa ctaacaaaat accaataata atatcactca tgttattcct ccgtccattt
      481 tgtttttctg ataagcaaac taatatatca gtcaaactca ttctaactta tgtagttagc
      541 gctttcatta aaggatgacc ataaatgttc agttttcaat taaaaaacct taattatgtt
      601 attttagact tatacgttat tgtcctgttt catttgatgg aggattgtaa ataaaaaaag
      661 aggaaagaac ataactttt
//
LOCUS       DAZUMV010000286          672 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000286 DAZUMV010000000
VERSION     DAZUMV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.307"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..>672
                     /locus_tag="ACG8FK_002948"
     CDS             102..>672
                     /locus_tag="ACG8FK_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS5098462.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSH"
BASE COUNT          209 a          131 c          128 g          204 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tg
//
LOCUS       DAZUMV010000287          654 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000287 DAZUMV010000000
VERSION     DAZUMV010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.308"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..>654
                     /locus_tag="ACG8FK_002949"
     CDS             51..>654
                     /locus_tag="ACG8FK_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS5098463.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYK"
BASE COUNT          238 a          107 c          130 g          179 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aaga
//
LOCUS       DAZUMV010000288          636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000288 DAZUMV010000000
VERSION     DAZUMV010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.309"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>636)
                     /locus_tag="ACG8FK_002950"
     rRNA            complement(<1..>636)
                     /locus_tag="ACG8FK_002950"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          128 a          209 c          134 g          165 t
ORIGIN      
        1 ccaacactta gcactcatcg tttacggcgt ggactaccag ggtatctaat cctgtttgct
       61 ccccacgctt tcgagcctca gcgtcagtta cagaccagag agccgccttc gccactggtg
      121 ttcctccata tatctacgca tttcaccgct acacatggaa ttccactctc ctcttctgca
      181 ctcaagtctc ccagtttcca atgaccctcc ccggttgagc cgggggcttt cacatcagac
      241 ttaagaaacc gcctgcgctc gctttacgcc caataaatcc ggacaacgct tgccacctac
      301 gtattaccgc ggctgctggc acgtagttag ccgtggcttt ctggttagat accgtcaagg
      361 gatgaacagt tactctcatc cttgttcttc tctaacaaca gagttttacg atccgaaaac
      421 cttcttcact cacgcggcgt tgctcggtca gactttcgtc cattgccgaa gattccctac
      481 tgctgcctcc cgtaggagtt tgggccgtgt ctcagtccca atgtggccga tcaccctctc
      541 aggtcggcta tgcatcgtgg ccttggtgag ccgttacctc accaactagc taatgcaccg
      601 cgggtccatc catcagcgac acccgaaagc gccttt
//
LOCUS       DAZUMV010000289          622 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000289 DAZUMV010000000
VERSION     DAZUMV010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.310"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..448)
                     /locus_tag="ACG8FK_002951"
     CDS             complement(<1..448)
                     /locus_tag="ACG8FK_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098464.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          181 a          113 c           86 g          242 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cg
//
LOCUS       DAZUMV010000290          588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000290 DAZUMV010000000
VERSION     DAZUMV010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.311"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG8FK_002952"
     tRNA            complement(65..150)
                     /locus_tag="ACG8FK_002952"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG8FK_002953"
     tRNA            complement(167..238)
                     /locus_tag="ACG8FK_002953"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG8FK_002954"
     tRNA            complement(250..322)
                     /locus_tag="ACG8FK_002954"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG8FK_002955"
     tRNA            complement(340..421)
                     /locus_tag="ACG8FK_002955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG8FK_002956"
     tRNA            complement(429..501)
                     /locus_tag="ACG8FK_002956"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG8FK_002957"
     tRNA            complement(505..577)
                     /locus_tag="ACG8FK_002957"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZUMV010000291          572 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000291 DAZUMV010000000
VERSION     DAZUMV010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.312"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>572
                     /locus_tag="ACG8FK_002958"
     CDS             <1..>572
                     /locus_tag="ACG8FK_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFS5098465.1"
                     /translation="DYIKRIDSDSFLAAFYLYTAKYWIDDSRKTIKYAKRKKQLYFQT
                     WHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKKYVSAFNIVPSKIL
                     EVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVDNLREFFK
                     KQQQELNFMTKFHPNVHSKLATDSKSIEYM"
BASE COUNT          222 a           79 c           92 g          179 t
ORIGIN      
        1 cagattatat taaaagaatt gactccgatt cttttttagc tgccttttat ttatatacag
       61 ctaagtattg gatagacgat agtcggaaaa cgattaagta tgcaaaaagg aaaaaacaac
      121 tttattttca aacatggcac ggaacgcctt taaaaaaaat tgaatttgat gcaaaagata
      181 agttacctaa acgttaccta agctatgcga aaaaagatag tgagtcaatt acatatttat
      241 tatctggtaa tcgctatagt tctaagaagt atgtttcagc attcaatatc gttccttcta
      301 aaatattaga agtaggaact cctagaaacg atgaattagc ctctagtaaa gagcaagtgt
      361 ttgacttgaa aaagaaacaa atcaatgtct tatttgctcc aacttttcga aataatattg
      421 aggacaacgg aattacgcaa ctagaatggt taggtgtgga taacttacgt gaatttttta
      481 aaaagcaaca acaagaatta aattttatga ctaaatttca tcctaatgtt catagtaaat
      541 tagcaactga ttctaaaagt atagaatata tg
//
LOCUS       DAZUMV010000292          563 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000292 DAZUMV010000000
VERSION     DAZUMV010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.313"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..463)
                     /locus_tag="ACG8FK_002959"
     CDS             complement(164..463)
                     /locus_tag="ACG8FK_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5098466.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          180 a          103 c           68 g          212 t
ORIGIN      
        1 tactagctgt ttttgtgtta attaaaggat aaaatacaat ataaagaaac atctgtttct
       61 ttatttagaa taaaaaaata cagactctgt tcaaataaat gaacctagcc tgcattatga
      121 gattgactat actttttaat tcattgagat gaataactcg tttttataaa tgcccaaaag
      181 taatcggtgc cgaagccatt cctcgaccta attctttttc aaattttcct attgttgata
      241 tctccatata aaacggtgat actggtttag acaattctcc cctgattgct aacggacgta
      301 aactacgttc caaattattt atgactttag gataatactg acctttttct aagcctgtct
      361 ttgcgtctat caaaagtata cgttcctttt ctttgatctc tggatttaaa acgagatcat
      421 ataattttag taccatgttt tctatttctt cacttttaga catcacgatt tcctcccaaa
      481 aatatatttc ttcccactac attctataaa caaagtatac gctacatcta gattttttta
      541 aatcattact ggattgttaa aac
//
LOCUS       DAZUMV010000293          560 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000293 DAZUMV010000000
VERSION     DAZUMV010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.314"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..293
                     /locus_tag="ACG8FK_002960"
     CDS             <1..293
                     /locus_tag="ACG8FK_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFS5098467.1"
                     /translation="LITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPI
                     ISVQDSYGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            290..>560
                     /locus_tag="ACG8FK_002961"
     CDS             290..>560
                     /locus_tag="ACG8FK_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5098468.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFL"
BASE COUNT          198 a           67 c           91 g          204 t
ORIGIN      
        1 ttttaatcac agattattca tctatttttt tcgattttgc gctaacaaaa aaaccaatta
       61 ttctttttaa ctatgatgaa aaagaatata gcttagaaag aggtttttat atacagttaa
      121 gtgagttacc aattataagc gtacaagata gttatggcct tgttgatgtt tttaaaaacc
      181 aacaagaaaa gcttttgaaa agttctcaag agttacttga ttatattgga aattatgaaa
      241 caggagcaag tacagaaaag gttcttgagt tgatagagag ggatataaag tgaaaaaaac
      301 agtaaaagat atattttata atgcgatata tcaagtcttt ttgattgttt tacccttact
      361 tactatacct atacttagtc gtagaattgg ttcaactggt ttagggattt atgggtatgt
      421 tttctcaata tcacagtttt taatgactgt cattgctgtg ggaatgaatc cttttcgtat
      481 tcgaaatatc gctaaatcaa ggaaagataa aaaagcacta tctttacaat tctggaatat
      541 ttactttatt caatttttaa
//
LOCUS       DAZUMV010000294          551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000294 DAZUMV010000000
VERSION     DAZUMV010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.315"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>551
                     /locus_tag="ACG8FK_002962"
     CDS             <1..>551
                     /locus_tag="ACG8FK_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5098469.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSN"
BASE COUNT          203 a           87 c           95 g          166 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt g
//
LOCUS       DAZUMV010000295          530 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000295 DAZUMV010000000
VERSION     DAZUMV010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.316"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..169)
                     /locus_tag="ACG8FK_002963"
     tRNA            complement(98..169)
                     /locus_tag="ACG8FK_002963"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(135..137),aa:Gln,seq:ttg)
     gene            complement(179..251)
                     /locus_tag="ACG8FK_002964"
     tRNA            complement(179..251)
                     /locus_tag="ACG8FK_002964"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(216..218),aa:His,seq:gtg)
     gene            complement(313..397)
                     /locus_tag="ACG8FK_002965"
     tRNA            complement(313..397)
                     /locus_tag="ACG8FK_002965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Leu,seq:caa)
     gene            complement(418..488)
                     /locus_tag="ACG8FK_002966"
     tRNA            complement(418..488)
                     /locus_tag="ACG8FK_002966"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(454..456),aa:Cys,seq:gca)
BASE COUNT          151 a          136 c          122 g          121 t
ORIGIN      
        1 gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc cagcgcgtct
       61 gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg attcgaacca
      121 acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa gaataaaggg
      181 cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt taaccacttc
      241 gccacattcg ccatcataaa agatgttatt taatttctca ataaaaaact aagatgaaga
      301 catcttagtc aataccggcg gccggggtcg aaccggcacg ccctcaacgg gcactggatt
      361 ttgagtccag cgcgtctgcc aattccgcca cgccggcaag ttgatttatt agtactaagg
      421 cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac ctctgcctta ccacttggct
      481 ataccgccat tatactatta tcaatctata aaataaagaa taaattatag
//
LOCUS       DAZUMV010000296          511 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000296 DAZUMV010000000
VERSION     DAZUMV010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.317"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..342
                     /locus_tag="ACG8FK_002967"
     CDS             <1..342
                     /locus_tag="ACG8FK_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098470.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           68 c           93 g          144 t
ORIGIN      
        1 gataacatac gagataaaaa ggcaagttta aaatcactaa aagatacatg gcttgattta
       61 tcagaagata atccatacag ccaattctta attactgtaa tggctggtgt taaccaatta
      121 gagcgagatc ttattcggat gagacaacgt gaagggattg aattggctaa gaaagaagga
      181 aagtttaaag gtcgattaaa gaagtatcat aaaaatcacg caggaatgaa ttatgcggta
      241 aagctatata aagaaggaaa tatgactgta aatcaaattt gtgaaattac taatgtatct
      301 agggcttcat tatacaggaa attatcagaa gtgaataatt agccattctg tattccgcta
      361 atgggcaata tttttaaaga agaaaaggaa actataaaat attaacagcc tcctagcgat
      421 gccgaaaagc cctttgataa aaaaagaatc atcatcttaa gaaattctta gtcatttatt
      481 atgtaaatgc ttataaattc ggccctataa t
//
LOCUS       DAZUMV010000297          488 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000297 DAZUMV010000000
VERSION     DAZUMV010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.319"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>488)
                     /locus_tag="ACG8FK_002968"
     CDS             complement(<1..>488)
                     /locus_tag="ACG8FK_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098471.1"
                     /translation="YVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAF
                     SLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGR
                     YNYKLSVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGK
                     PF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaacggct ttccaaatga tgaaatacct aaatctaact tattaagaaa gttgaaattt
       61 ttatattcag gaaaccattt attaatttca tgtgcagcac gtaatgcatt ccctggagtt
      121 gtcaaagaga aaattaattc tgctaagctt attattgaaa atactgataa cttataatta
      181 tatcgtccat tgacaatcaa atataaacta actatacttg ttagaacaaa aaaacaggcg
      241 ttgacttgtt cttgattagt taaaaatatc aataaaggaa tacttaccca atatattttt
      301 ctatttattt cttctcctct taagagttga aaaaacggat aaaaagttag taaactaaat
      361 gctactggcc actgatagtt caaggtggtt gctctccaac cggtttcccc cattactgtc
      421 aatggtatta aacagtatat agagcaaacc aataataaat ttttaacatt tattttctca
      481 aaaacata
//
LOCUS       DAZUMV010000298          485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000298 DAZUMV010000000
VERSION     DAZUMV010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.320"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>485
                     /locus_tag="ACG8FK_002969"
     CDS             <1..>485
                     /locus_tag="ACG8FK_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS5098472.1"
                     /translation="KVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDA
                     KWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKE
                     DLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTV
                     EG"
BASE COUNT          197 a           74 c          113 g          101 t
ORIGIN      
        1 aaagtaacct tcaccgcagg agaaggaaca agtattgaaa gtggaacaac agtctttgca
       61 gtgaaagacg gcgtaagctt accagaagat aaacttccag tgttgaaagc aaaagatggt
      121 tatacagatg cgaaatggcc agaggaagca actcaaccaa ttacagcaga tgatacagaa
      181 tttgtatcaa gtgcaacaaa actggatgat aaatctgacg ctgacaaata taatcctgaa
      241 ggtcaaaaag tgactacaga attgaataag gaaccagacg catttgaggg aattaagaac
      301 aaagaagatt taccaaaaga tactaagtat acttggaaag aaaaagtaga tgttagcgca
      361 gctggaaata aaaaagggac tgttgtagtg acatatccag atggatcatc tgatgaagtt
      421 gaagtagatg ttacagtgac agacaatcgt tctgacgccg ataaatatga gccaacagta
      481 gaagg
//
LOCUS       DAZUMV010000299          474 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000299 DAZUMV010000000
VERSION     DAZUMV010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.321"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..171
                     /locus_tag="ACG8FK_002970"
     CDS             <1..171
                     /locus_tag="ACG8FK_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5098473.1"
                     /translation="KETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKATTCIAGMSI
                     GGFLGGAIPGKC"
     gene            173..>474
                     /locus_tag="ACG8FK_002971"
     CDS             173..>474
                     /locus_tag="ACG8FK_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5098474.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKS"
BASE COUNT          187 a           50 c           91 g          146 t
ORIGIN      
        1 aaagagacac aacttatcta tgggggtacc actcatagtg gaaaatatta tggaaatgga
       61 gtgtattgca ctaaaaataa atgtacggtc gattgggcca aggcaactac ttgtattgca
      121 ggaatgtcta taggtggttt tttaggtgga gcaattccag ggaagtgcta aaatgaaaaa
      181 aaatgctaag caaattgttc atgaattata taatgatata tctataagta aagatcctaa
      241 atattctgat attcttgagg ttttacaaaa ggtatattta aaattagaaa aacaaaaata
      301 tgaattagat cccagtcctt taataaatag attggtgaat tatctatatt ttactgctta
      361 tactaataaa ataagattca ctgaatatca agaggaatta ataagaaatt tgagtgaaat
      421 tggaagaact gctggaataa atggtttata tcgagcagat tatggagata aatc
//
LOCUS       DAZUMV010000300          470 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000300 DAZUMV010000000
VERSION     DAZUMV010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.322"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..136)
                     /locus_tag="ACG8FK_002972"
     CDS             complement(<1..136)
                     /locus_tag="ACG8FK_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5098475.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     K"
     gene            complement(155..316)
                     /locus_tag="ACG8FK_002973"
     CDS             complement(155..316)
                     /locus_tag="ACG8FK_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS5098476.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          157 a           95 c           55 g          163 t
ORIGIN      
        1 ccttttctat ttcattgctt gatttcatta ttgttaaaca agaatacaag caagaaagaa
       61 ctatagttaa tatgataaaa attattaaag ttctattttt ctttcttgta atatatgcta
      121 tagcattttt tatcactttc ctaactactc ctttctattt tcttactcca taactaattg
      181 catccactgg acaagcattt ttacatcgtc cacatctgat acactccaga tgattgcaat
      241 tctcaactga atctttattc atttggcaaa cttttttaca ttttccacaa ttaatacact
      301 tatccttatc ctgaatcggc tacactagac tagacagaaa aaataaggtg tgtagaataa
      361 taaaaaacac actggaggat ttttcatgcc aagacgtcaa cgaagaacct attcaaaaga
      421 attcaaacaa caaatcgtcg atctctatct cgctggtaag ccttgcgcag
//
LOCUS       DAZUMV010000301          464 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000301 DAZUMV010000000
VERSION     DAZUMV010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.323"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          150 a           91 c           56 g          167 t
ORIGIN      
        1 cggcgaaagc acaaaaaata aaaaaattat tacgttatta aaaaaacaga tgaaagatca
       61 ggttttatta gatctcattc tttcatcact tgaagggatt taatgaacta ctttttcctc
      121 tttgtcgttt ttatatattc gtattccttc aaacagtagg atagcctctt tcaatgcttg
      181 atatttgaac tgtccgttac ttaaaaattt agatgtgccg ccctcttcct agaacatatt
      241 cttttttact ttttcattaa ttgacagacc aatcgaacga aactgtaaat ttcgatagga
      301 taatgattgg tcaaactcat tcattttcaa gacatctcga ttcatattca agataggaat
      361 ctttttatca acatcacttt attcgtattt tcctctacca taaaagcttc acgctccaca
      421 aaattgtttt atacactact attcagccca ttcaacgacc aatg
//
LOCUS       DAZUMV010000302          455 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000302 DAZUMV010000000
VERSION     DAZUMV010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.324"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG8FK_002974"
     CDS             complement(<1..430)
                     /locus_tag="ACG8FK_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5098477.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          131 a           90 c           58 g          176 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatt
//
LOCUS       DAZUMV010000303          451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000303 DAZUMV010000000
VERSION     DAZUMV010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.325"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>451)
                     /locus_tag="ACG8FK_002975"
     CDS             complement(<1..>451)
                     /locus_tag="ACG8FK_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5098478.1"
                     /translation="QNELNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKE
                     LSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSV
                     NPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYV"
BASE COUNT          164 a           84 c           62 g          141 t
ORIGIN      
        1 tacataataa ccagtagaac ggtactgcat aagcagtcgg aatagagttt ttgcatacgt
       61 agaacgcaac gacgtgaaag cagataattc aaatctacta aattcagctg ttaaaccatt
      121 aataatatat tctaggtccg gattaacact gacttcaaca aacttttttg ttttattgat
      181 ttcaaaacca gtaaacaaaa caaagtagcg aatagaatcc tcatcttcag tacggtaagt
      241 aagattaagc atatctttat agagcttttc taaatcaccg acaaaggctt ttgtagcagt
      301 catcttataa tcacttaact cttttaattc ctcgaaagaa aatctaactt ttttcaaacc
      361 acgatctttc atacgagcgc ataatgcaaa aaataaatcc atctgcttag cattgaaatt
      421 tttcagcggt actaaattca attcattttg a
//
LOCUS       DAZUMV010000304          432 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000304 DAZUMV010000000
VERSION     DAZUMV010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.326"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..379
                     /locus_tag="ACG8FK_002976"
     CDS             44..379
                     /locus_tag="ACG8FK_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098479.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          168 a           52 c           80 g          132 t
ORIGIN      
        1 aatttcctag gaaaaatagt taacataaaa ggaatgaatt gttatgaaac aatatttaaa
       61 tgaatggaaa gcgatagagg ggtcattagt aacgcaaaga ataaaggaat tacctgattg
      121 cttagagaag gatcatttgt ttcaaatacg agaaatgttg aaaaaagaac agtttgatcc
      181 agaccaattt ttagtagtcg agtatccaac tattggagtg tactgttgta accatatcaa
      241 tgatggaaaa tattttatta ttcaggagta tggaggacag ttaactcctt tctatactac
      301 atgggaaatg agtgaagaag gaatcaataa ttttccatgt gaaagcatcg aagattcaat
      361 ttcacacacc gaatgttaat tgattataac tataaaaaag gattgaacat tatgttcaat
      421 ccttttttca at
//
LOCUS       DAZUMV010000305          424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000305 DAZUMV010000000
VERSION     DAZUMV010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.327"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>424
                     /locus_tag="ACG8FK_002977"
     CDS             <1..>424
                     /locus_tag="ACG8FK_002977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098480.1"
                     /translation="LVTNILLGSQWKEAVPFVGIYGLMSCFTLVLGQYASEYYRGLGK
                     PKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRSFVKIEQIIVFWIILWFGFKFNPF
                     KILKQVWKSCASALLMGVLAKIMLEFCTNYVMQLLSVCL"
BASE COUNT          125 a           54 c           85 g          160 t
ORIGIN      
        1 ctagtaacaa atattctact cgggagtcaa tggaaagagg ctgtaccatt tgttgggata
       61 tatggattaa tgagctgttt tactcttgtc ttgggacaat atgcctctga gtactatcgt
      121 ggtttaggaa aaccaaaagc aaatgttctt atgactatcc tacatttagc agtattaatt
      181 ccagttctaa taatctattc aaggaaaggg tttatagaac tagcatatgg tagatctttc
      241 gttaagatag agcagattat agtcttttgg attattttat ggtttggttt taagtttaat
      301 ccatttaaaa ttttaaagca ggtttggaaa tcttgtgcta gtgcattgct aatgggagtt
      361 ctagcaaaaa ttatgttgga attctgtact aattatgtta tgcaattact ttcagtgtgt
      421 cttt
//
LOCUS       DAZUMV010000306          415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000306 DAZUMV010000000
VERSION     DAZUMV010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.328"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG8FK_002978"
     CDS             complement(<1..339)
                     /locus_tag="ACG8FK_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098481.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAZUMV010000307          385 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000307 DAZUMV010000000
VERSION     DAZUMV010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.329"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..>385
                     /locus_tag="ACG8FK_002979"
     CDS             87..>385
                     /locus_tag="ACG8FK_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5098482.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSV"
BASE COUNT          144 a           66 c           58 g          117 t
ORIGIN      
        1 gaaaacaggt ctatacttat tcaatggaat cgtaccagtt ccaagttttt aagctcgtac
       61 caagcttaga aagaagaggc aatcgtatga aaataacaga aaagaatgta aacccgaaat
      121 ttgaaactac cgaaagcaat ctaaaaaagt ttgatttaac tgttacttta tcgaatcaac
      181 ttgtagatag cattgttgaa atggatgcta tgccacaaaa aatttatctt tacgctatag
      241 gacatcttga ccctaaaaat ccgcctttta ataataccgt caaagtatct aaaaaggctt
      301 tatttgcgtt gatgtcttct agttcatctt cttcaaaaca ttcaagatta aaacaagtta
      361 ttgaaaaatt gcatcagcaa tctgt
//
LOCUS       DAZUMV010000308          368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000308 DAZUMV010000000
VERSION     DAZUMV010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.330"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>368
                     /locus_tag="ACG8FK_002980"
     CDS             82..>368
                     /locus_tag="ACG8FK_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS5098483.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          130 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcg
//
LOCUS       DAZUMV010000309          365 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000309 DAZUMV010000000
VERSION     DAZUMV010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.331"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            193..>365
                     /locus_tag="ACG8FK_002981"
     CDS             193..>365
                     /locus_tag="ACG8FK_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5098484.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT          152 a           55 c           50 g          108 t
ORIGIN      
        1 gattatttat ttcatcctag ttttagccaa ccctataatt ttattttaaa ggcagtaaca
       61 ggattaaaca ttgaaacata atcgttacta aatgaaatca agaaatatac tgtattactc
      121 aattcattga aaatatttat cataattttt aatagaaaac actattaaag agaaaaaaga
      181 gaggtaaatc aaatgaaaaa atctttaaaa cttgcatcag cagttatatt aatgtcccaa
      241 ctccttatag caacaccttt ccatgtgttg gcaagcgaaa attcaacata caaaattgca
      301 aaagaaagaa ccgagacgac tgaaatgagt aattccgatg taacagcaga gagtagtgaa
      361 ctttt
//
LOCUS       DAZUMV010000310          356 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000310 DAZUMV010000000
VERSION     DAZUMV010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.332"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>356)
                     /locus_tag="ACG8FK_002982"
     rRNA            complement(<1..>356)
                     /locus_tag="ACG8FK_002982"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           79 a          105 c           87 g           85 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcgg
//
LOCUS       DAZUMV010000311          335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000311 DAZUMV010000000
VERSION     DAZUMV010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.333"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          125 a           54 c           59 g           97 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgatagg ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaata
//
LOCUS       DAZUMV010000312          332 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000312 DAZUMV010000000
VERSION     DAZUMV010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.334"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>332
                     /locus_tag="ACG8FK_002983"
     CDS             <1..>332
                     /locus_tag="ACG8FK_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098485.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAZUMV010000313          324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000313 DAZUMV010000000
VERSION     DAZUMV010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.335"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..>324
                     /locus_tag="ACG8FK_002984"
     CDS             71..>324
                     /locus_tag="ACG8FK_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5098486.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           95 a           57 c           62 g          110 t
ORIGIN      
        1 tttatggatg atgtctacag tcttttcttt tataatagat ttatgaaaac gaatgcaagg
       61 agtgatagct atgatgtcta aaaacaatac gaatggtaga aatcaatttg cgatgcttac
      121 gattgatgat ctagttcctc aagatcatct cgttcgaaag attgatgccg cgcttgattt
      181 tgaatttatt tatccaatcg tcgaagccac ttactccgat ttaggtcgcc caagtatcga
      241 tccagtgatt ctgatcaaat tggtgtttat tcagtattta ttcggtatcc gttccatgcg
      301 acaaaccatt aaggaagtcg atac
//
LOCUS       DAZUMV010000314          323 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000314 DAZUMV010000000
VERSION     DAZUMV010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.336"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8FK_002985"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG8FK_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS5098487.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAZUMV010000315          321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000315 DAZUMV010000000
VERSION     DAZUMV010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.337"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8FK_002986"
     CDS             complement(<1..>321)
                     /locus_tag="ACG8FK_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5098488.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZUMV010000316          310 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000316 DAZUMV010000000
VERSION     DAZUMV010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.339"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          126 a           32 c           54 g           98 t
ORIGIN      
        1 taaagagatt gctatattat tatttgttct aaatgattcc tttaaggtgt cactaaaaat
       61 tataaaaaat agtgttgacg cacttttgat gtcatgttat aataacgaag tcgtttggat
      121 gagatagcta ataaagcttg attttatcga aaaaaacttc gaaaaagtta ttgacaaata
      181 acaataaaga tggtatgata atcaagtcgc tgctaatgag tggcaaagac gtttagaaaa
      241 aataaacaaa gaagttgttg acattctctt ctgaatctga taagattaaa gagttgttaa
      301 aacaaaaaga
//
LOCUS       DAZUMV010000317          297 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000317 DAZUMV010000000
VERSION     DAZUMV010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.341"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          105 a           52 c           43 g           97 t
ORIGIN      
        1 ctctataatt tctaggactg atggaaattt tctatctgtc ctagtttttt tgtaaaaaaa
       61 caaaaagaca tacaaaattt ccggtagaat agagtctcct acaacatcat tctgggggaa
      121 ttcttgtatg tccatgaatc attttatcaa acaactcttt actattaaag actcaaatat
      181 ccaaattcaa tcctttgaag aaacttttta taaaagcaga aaaagtatcg ttattgaggg
      241 aactctgatc cgtacctaca gacgttgtcc gtgttgtaaa gattctacaa agcaaat
//
LOCUS       DAZUMV010000318          291 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000318 DAZUMV010000000
VERSION     DAZUMV010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.342"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..>291)
                     /locus_tag="ACG8FK_002987"
     CDS             complement(119..>291)
                     /locus_tag="ACG8FK_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5098489.1"
                     /translation="NKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQ
                     QVTDTLVSMFTE"
BASE COUNT           87 a           39 c           50 g          115 t
ORIGIN      
        1 cgcactcttt atttagtttt tatgcaaaaa atcaagttat aagataaagt aggtgggatt
       61 ttgttaggga gcgtcaataa ttttgtgtaa ataaattgtc ctcctgcaaa ataattagtt
      121 actcagtaaa cattgaaact aatgtatcgg ttacctgttg aaaaccttta tggcttctgt
      181 ttagaaattt ttgattgtat gtatcaaaaa tgctgactag aaagcgttct agtgattctt
      241 cattttgaaa ctgctctttt ctacggctgt atcttttaat ttgcttattg a
//
LOCUS       DAZUMV010000319          291 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000319 DAZUMV010000000
VERSION     DAZUMV010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.343"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACG8FK_002988"
     CDS             <1..>291
                     /locus_tag="ACG8FK_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5098490.1"
                     /translation="AIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVK
                     KAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT           93 a           47 c           59 g           92 t
ORIGIN      
        1 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
       61 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      121 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      181 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      241 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt a
//
LOCUS       DAZUMV010000320          289 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000320 DAZUMV010000000
VERSION     DAZUMV010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.344"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>289
                     /locus_tag="ACG8FK_002989"
     CDS             <1..>289
                     /locus_tag="ACG8FK_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5098491.1"
                     /translation="HKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNF
                     FKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGL"
BASE COUNT           92 a           59 c           48 g           90 t
ORIGIN      
        1 tcataaaagt gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa
       61 acccaacggc cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt
      121 ccctaaaaac ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa
      181 agccattagt aaccaacatg atattgatat atattttgcg gaccctggaa caccttctca
      241 acgcccatta aacgagaatt ctaacgggat tctgcgtcgt aatggactg
//
LOCUS       DAZUMV010000321          282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000321 DAZUMV010000000
VERSION     DAZUMV010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.345"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..243
                     /locus_tag="ACG8FK_002990"
     CDS             <1..243
                     /locus_tag="ACG8FK_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5098492.1"
                     /translation="CASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKII
                     RIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          103 a           54 c           50 g           75 t
ORIGIN      
        1 tgcgcatcat tggatgatgg attcgaagac gcctttcaat ataccgtaca aggaaattcc
       61 cacaatcgac taaagagtac caatctaatt gaacgactga atcaagaagt acgcagaaga
      121 gaaaagatta ttcgcatctt ccccaatcaa acatcagcca atcgcttaat tggagccgtt
      181 cttatggacc tacatgatga atggatttat tcttcaagaa aatacatcaa ttttgataag
      241 tagaaatggt aaaaacattg tatagcattt tacacaggag tc
//
LOCUS       DAZUMV010000322          274 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000322 DAZUMV010000000
VERSION     DAZUMV010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.346"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>274)
                     /locus_tag="ACG8FK_002991"
     CDS             complement(<1..>274)
                     /locus_tag="ACG8FK_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5098493.1"
                     /translation="IHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSS
                     LEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSL"
BASE COUNT           80 a           50 c           48 g           96 t
ORIGIN      
        1 taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata gaacactgta
       61 agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg agttgaaatt
      121 ttgacaacct aatttttcta aggaagaaaa aacagtctca actgtttttc tttgttttcc
      181 aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg gaacggatat
      241 agtaatgccc ttaagggcaa gcttttcatg gatt
//
LOCUS       DAZUMV010000323          268 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000323 DAZUMV010000000
VERSION     DAZUMV010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.348"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..203
                     /locus_tag="ACG8FK_002992"
     CDS             <1..203
                     /locus_tag="ACG8FK_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5098494.1"
                     /translation="PEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKL
                     EFTFSSLTSDLPKISLRNWLEE"
BASE COUNT          114 a           52 c           36 g           66 t
ORIGIN      
        1 tcccagaata ctatcaaatg ggtaacattg atcaaaaagt attaaatcct gcattacgag
       61 aactaaaaaa atacttcaat gatcttaaag tcacaaaaat aaaagcaaaa aagggcaata
      121 aaatcgccaa attagaattt actttttcaa gcctaacgtc agatttgcca aagatttctc
      181 tgcgtaactg gttagaagaa taaataaaaa agaactcgcc ccccttacat taagcaagta
      241 gacaagtccc ttacaaccga ctaagatt
//
LOCUS       DAZUMV010000324          265 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000324 DAZUMV010000000
VERSION     DAZUMV010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.349"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>265
                     /locus_tag="ACG8FK_002993"
     CDS             <1..>265
                     /locus_tag="ACG8FK_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFS5098495.1"
                     /translation="KAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELED
                     EINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          106 a           44 c           61 g           54 t
ORIGIN      
        1 aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat caaagctaac
       61 gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc ctatcaggat
      121 gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc ttttactttg
      181 gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga tccggaaagc
      241 ggctactacg tcaaaggcga acgag
//
LOCUS       DAZUMV010000325          262 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000325 DAZUMV010000000
VERSION     DAZUMV010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.denovo.351"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..241)
                     /locus_tag="ACG8FK_002994"
     CDS             complement(<1..241)
                     /locus_tag="ACG8FK_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5098496.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYI"
BASE COUNT           67 a           50 c           48 g           97 t
ORIGIN      
        1 caatgtattc tttttcctca gcaggaaaaa tgatttcggt tctcccacaa cgtcgcttat
       61 tttctttgta ttgttcaaag tattcaagtg ctgttccacc acatttgaga aagttataga
      121 cattgtaaat tgtttggcgt ccacgtttaa gctgcttcgc aacgatagca accggagttt
      181 cttgatgaaa atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca
      241 tttatgttca ctctccttgt at
//
LOCUS       DAZUMV010000326        10748 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.092, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000326 DAZUMV010000000
VERSION     DAZUMV010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.092"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..850
                     /gene="vanY"
                     /locus_tag="ACG8FK_002995"
     CDS             <1..850
                     /gene="vanY"
                     /locus_tag="ACG8FK_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFS5098497.1"
                     /translation="LFDSRFKTLQQNQNYDQNPKEHLENSGTSENTQEKTITEEQVYQ
                     GNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDSNIYMSKEIAQKFSEMVNDA
                     VKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEHNSGLSLDVGSSLTKMERA
                     PEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIRYVGLPHSAIMKEKNFVLEEYM
                     DYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEISGNNIDGVIVTVFPGST
                     HTNSRR"
     gene            1003..1488
                     /gene="vanZ-A"
                     /locus_tag="ACG8FK_002996"
     CDS             1003..1488
                     /gene="vanZ-A"
                     /locus_tag="ACG8FK_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFS5098498.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2007..2291
                     /locus_tag="ACG8FK_002997"
     CDS             2007..2291
                     /locus_tag="ACG8FK_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5098499.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2657..2956
                     /locus_tag="ACG8FK_002998"
     CDS             2657..2956
                     /locus_tag="ACG8FK_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098500.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            2999..4490
                     /locus_tag="ACG8FK_002999"
                     /pseudo
     CDS             2999..4490
                     /locus_tag="ACG8FK_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
     gene            4838..5008
                     /locus_tag="ACG8FK_003000"
     CDS             4838..5008
                     /locus_tag="ACG8FK_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098501.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            5022..5639
                     /locus_tag="ACG8FK_003001"
     CDS             5022..5639
                     /locus_tag="ACG8FK_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5098502.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            5639..6577
                     /locus_tag="ACG8FK_003002"
                     /pseudo
     CDS             5639..6577
                     /locus_tag="ACG8FK_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(7270..7917)
                     /locus_tag="ACG8FK_003003"
     CDS             complement(7270..7917)
                     /locus_tag="ACG8FK_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFS5098503.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(8047..8985)
                     /locus_tag="ACG8FK_003004"
     CDS             complement(8047..8985)
                     /locus_tag="ACG8FK_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5098504.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            9476..10693
                     /locus_tag="ACG8FK_003005"
                     /pseudo
     CDS             9476..10693
                     /locus_tag="ACG8FK_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         3924 a         1660 c         1860 g         3304 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattatgatc aaaatcccaa
       61 agaacattta gaaaatagtg ggacttctga aaatacccaa gagaaaacaa ttacagaaga
      121 acaggtttat caaggaaatc tgctattaat caatagtaaa tatcctgttc gccaagaaag
      181 tgtgaagtca gatatcgtga atttatctaa acatgacgaa ttaataaatg gatacgggtt
      241 gcttgatagt aatatttata tgtcaaaaga aatagcacaa aaattttcag agatggtcaa
      301 tgatgctgta aagggtggcg ttagtcattt tattattaat agtggctatc gagactttga
      361 tgagcaaagt gtgctttacc aagaaatggg ggctgagtat gccttaccag caggttatag
      421 tgagcataat tcaggtttat cactagatgt aggatcaagc ttgacgaaaa tggaacgagc
      481 ccctgaagga aagtggatag aagaaaatgc ttggaaatac gggttcattt tacgttatcc
      541 agaggacaaa acagagttaa caggaattca atatgaacca tggcatattc gctatgttgg
      601 tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta
      661 cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg gaaaaatatg agatctttta
      721 ttatcctgtt actaaaaata ccaccattca tgtgccgact aatcttcgtt atgagatatc
      781 aggaaacaat atagacggtg taattgtgac agtgtttccc ggatcaacac atactaattc
      841 aaggaggtaa ggatggcgga atgaaaccaa cgaaattaat gaacagcatt attgtactag
      901 cacttttggg gtaacgttag ctttttaatt taaaacccac gttaactagg acattgctat
      961 actaatgata caacttaaac aaaagaatta gaggaaatta tattgggaaa aatattatct
     1021 agaggattgc tagctttata tttagtgaca ctaatctggt tagtgttatt caaattacaa
     1081 tacaatattt tatcagtatt taattatcat caaagaagtc ttaacttgac tccatttact
     1141 gctactggga atttcagaga gatgatagat aatgttataa tctttattcc atttggcttg
     1201 cttttgaatg tcaattttaa agaaatcgga tttttaccta agtttgcttt tgtactggtt
     1261 ttaagtctta cttttgaaat aattcaattt atcttcgcta ttggagcgac agacataaca
     1321 gatgtaatta caaatactgt tggaggcttt cttggactga aattatatgg tttaagcaat
     1381 aagcatatga atcaaaaaaa attagacaga gttattattt ttgtaggtat acttttgctc
     1441 gtattattgc tcgtttaccg tacccattta agaataaatt acgtgtaaga tgtctaaatc
     1501 aagcaatctg atctttcata cacataaaga tattgaatga attggattag atggaaaacg
     1561 ggatgtgggg aaactcgccc gtaggtgtga agtgagggga aaaccggtga taaagtaaaa
     1621 agcttaccta acactatagt aacaaagaaa gcccaattat caattttagt gctgaggaat
     1681 tggtctcttt aataaatttc cttaacgttg taaatccgca ttttcctgac ggtaccccca
     1741 ctagcagcca tgcttttttt gaatctttgt aggacgtttt aaagagaatt acccacttta
     1801 aaattaaaca tgcgcttaga atcgctttag gaaacacgat ccagtccaat aatcgtcgat
     1861 aaaaactttt gaaaaaggtt ggtgaaatta cctacttttg gaataatcac aaatcacaag
     1921 tgattaatca cttgtttatt aagatattaa aagctataat ttaaataaag cgtgaatttt
     1981 attacacaaa aagagggggg agaaacttgg aactagcatt tagagaaagc ttaaaaaaga
     2041 tgagaggtac caaatcaaaa gaaaaattct cccaagaatt agaaatgagt agatcaaatt
     2101 attcacgaat agaatcagga aaatcagatc caaccataaa aacactagaa caaattgcaa
     2161 agttaactaa ctcaacgcta gtagtggatt taatcccaaa tgagccaaca gaaccagaac
     2221 cagaaacaga atcagaacaa gtaacattgg atttagaaat ggaagaagaa aaaagcaatg
     2281 acttcgtgtg aataatgcac gaaatcgttg cttatttttt ttaaaagcgg tatactagat
     2341 ataacgaaac aacgaactga atagaaacga aaaaagagcc atgacacatt tataaaatgt
     2401 ttgacgacat tttataaatg catagcccga taagattgcc aaaccaacgc ttatcagtta
     2461 gtcagatgaa ctcttccctc gtaagaagtt atttaattaa ctttgtttga agacggtata
     2521 taaccgtact atcattatat agggaaatca gagagttttc aagtatctaa gctactgaat
     2581 ttaagaattg ttaagcaatc aatcggaaat cgtttgattg ctttttttgt attcatttat
     2641 agaaggtgga gtttgtatga atcatgatga atgtaaaact tatataaaaa atagtttatt
     2701 ggagataaga aaattagcaa atatctatac actagaaacg tttaagaaag agttagaaaa
     2761 gagaaatatc tacttagaaa caaaatcaga taagtatttt tcttcggagg gggaagatta
     2821 tatatataag ttaatagaaa ataacaaaat aatttattcg attagtggaa aaaaattgac
     2881 ttataaagga aaaaaatctt tttcaaaaca tgcaatattg aaacagttga atgaaaaagc
     2941 aaaccaagtt aattaaacaa cctattttat aggatttata ggaaaggaga acagctgaat
     3001 gaatatccct tttgttgtag aaactgtgct tcatgacggc ttgttaaagt acaaatttaa
     3061 aaatagtaaa attcgctcaa tcactaccaa gccaggtaaa agcaaagggg ctatttttgc
     3121 gtatcgctca aaatcaagca tgattggcgg tcgtggtgtt gttctgactt ccgaggaagc
     3181 gattcaagaa aatcaagata catttacaca ttggacaccc aacgtttatc gttatggaac
     3241 gtatgcagac gaaaaccgtt catacacgaa aggacattct gaaaacaatt taagacaaat
     3301 caataccttc tttattgatt ttgatattca cacggcaaaa gaaactattt cagcaagcga
     3361 tattttaaca accgctattg atttaggttt tatgcctact atgattatca aatctgataa
     3421 aggttatcaa gcatattttg ttttagaaac gccagtctat gtgacttcaa aatcagaatt
     3481 taaatctgtc aaagcagcca aaataatttc gcaaaatatc cgagaatatt ttggaaagtc
     3541 tttgccagtt gatctaacgt gtaatcattt tggtattgct cgcataccaa gaacggacaa
     3601 tgtagaattt tttgatccta attaccgtta ttctttcaaa gaatggcaag attggtcttt
     3661 caaacaaaca gataataagg gctttactcg ttcaagtcta acggttttaa gcggtacaga
     3721 aggcaaaaaa caagtagatg aaccctggtt taatctctta ttgcacgaaa cgaaattttc
     3781 aggagaaaag ggtttaatag ggcgtaataa cgtcatgttt accctctctt tagcctactt
     3841 tagttcaggc tattcaatcg aaacgtgcga atataatatg tttgagttta ataatcgatt
     3901 agatcaaccc ttagaagaaa aagaagtaat caaaattgtt agaagtgcct attcagaaaa
     3961 ctatcaaggg gctaataggg aatacattac cattctttgc aaagcttggg tatcaagtga
     4021 tttaaccagt aaagatttat ttgtccgtca agggtggttt aaattcaaga aaaaaagaag
     4081 cgaacgtcaa cgtgttcatt tgtcagaatg gaaagaagat ttaatggctt atattagcga
     4141 aaaaagcgat gtatacaagc cttatttagt gacgaccaaa aaagagatta gagaagtgct
     4201 aggcattcct gaacggacat tagataaatt gctgaaggta ctgaaggcga atcaggaaat
     4261 tttctttaag attaaaccag gaagaaatgg tggcattcaa cttgctagtg ttaaatcatt
     4321 gttgctatcg atcattaaag taaaaaaaga agaaaaagaa agctatataa aggcgctgac
     4381 aaattctttt gacttagagc atacattcca ttcaagagac tttaaacaag ctagcagaac
     4441 gccctaaaac ggacacacaa ctcgatttgt ttagctatga tacaggctga aaataaaacc
     4501 cgcactatgc cattacattt atatctatga tacgtgtttg ttttttcttt gctgtttagc
     4561 gaatgattag cagaaatata cagagtaaga ttttaattaa ttattagggg gagaaggaga
     4621 gagtagcccg aaaactttta gttggcttgg actgaacgaa gtgagggaaa ggctactaaa
     4681 acgtcgaggg gcagtgagag cgaagcgaac acttgatttt ttaattttct atcttttata
     4741 ggtcattaga gtatacctat ttgtcctaat atgattttag cagtataatt gacttggtga
     4801 ataggtcatt taagttgggc ataataggag gagtaaaatg aaaaaattta tttatcgagt
     4861 tttagaaaat gacgaagtgg tggctatttt taatgagcaa caatatgcgc aagattttat
     4921 cgcttacgaa aagacaattt ctgataagca atttgaaatt gaaaaagtag atattgctga
     4981 ttggttattg caaccgagag aattttagag gttggttgaa aatggctaaa attggttatg
     5041 cacgtgtcag tagcaaagaa cagaacttag atcggcaatt acaagcgtta cagggcgttt
     5101 ctaaggtctt ttcagacaaa ttaagcggtc aatcggtcga acgcccacaa ttacaagcta
     5161 tgcttaacta tattcgtgaa ggggatattg ttattgttac tgaattagat cgattaggac
     5221 gaaataataa agaattaaca gaattgatga atcaaattca aattaagggg gcaaccctgg
     5281 aagtcttaaa tttaccctca atgaatggta ttgaagatga aaatttaagg cgtttgatta
     5341 atagccttgt cattgaattg tacaagtatc aagcagaatc agaacgaaaa aaaattaagg
     5401 aacgtcaggc acaaggaatc gaaattgcta agaaaaaagg caaattcaaa ggtcgtcagc
     5461 ataaatttaa agaaaatgat ccacgtttaa agcatgcttt cgatttgttt ttgaatggtt
     5521 tatccgataa agaagttgaa gaacaaactg gaatcaatcg ccgaacgttt agaaggtatc
     5581 gagcaagata taacgtgaca gtcgatcaaa gaaaaaacaa tgaaaagagg gatagttaat
     5641 gagtacggtt attttagctg aaaaaccaag ccaggcatta gcctacgcaa gtgctttaaa
     5701 acaaagcacc aaaaaagacg gttattttga gatcaaagac ccactattta cagatgaaac
     5761 gtttatcacc tttggttttg ggcatttagt ggaattagca gaaccaggtc attatgacga
     5821 aaagtggcaa aattggaaac ttgaatcttt gccgattttt cctgatcgat acgattttga
     5881 agttgcaaaa gataagggaa agcagtttaa aattgttgca gaacttctca aaaaggcaaa
     5941 tacaattatt gttgcaacag atagcgacag agaaggtgaa aatatcgcct ggtcgattat
     6001 ccataaagca aatgcctttt caaaagataa aacatttaaa agactatgga tcaatagctt
     6061 agaaaaagat gtaatccgaa gcggttttca aaatttgcaa cctggaatga attactatcc
     6121 cttttatcaa gaagcgcaaa cacgccaaat tgccgattgg ttgatcggca tgaacgcaag
     6181 ccctttgtat acgttaaatt tacaacagaa gggcgtacaa ggtacatttt cactaggacg
     6241 tgttcaaacg cccaccttat accttatttt tcagcgccag gaagccatag agaattttaa
     6301 aaaagaacct tttttcgagg tggaagctag tataaaagta aaccaagggt cgtttaaggg
     6361 cgttctaagc cccacacagc gttttaaaac ccaagaggag cttttagctt ttgtttcttc
     6421 taaacaagct aaaataggca atcaagaggg gataattgct gatgttcaaa ccaaagagaa
     6481 gaaaacgaat agtccgagtt tgttttcttt aagtagtttg caatcaaaag tcaatcagct
     6541 ttataaagcg acagcgagcc aaactttaaa agctatttct tttttaataa cttaaaaata
     6601 aacttaatgt aacagcaagc acagtcaagg tatacacctt tgacaaaaaa tagcacattc
     6661 tctatcgaaa atttttgctt attttttaaa ttattttggg aaattttccc aatccctttt
     6721 tctaactcaa aaaatataat cactcaaaat ttaaaagggc gcacttatac atcattttaa
     6781 aaaattgatg taacgtgcta agttcaaaac aaagggcgca cttatacacg attttcaatc
     6841 ttgtatattt ctaacgaaaa gcgtgcgcca aaaaaccccc ttcgtcaatt ttgacagggg
     6901 gctttttgat gtaaaaattt ctatcgaaat ttaaaaattc gcttcactca tgttataaag
     6961 acttaaaata aaataactct ttaaaatctt ttgctagttg ttcttcaata ttttttattc
     7021 ggtgcatctt ccaagtaaag tataacacac tagacttatt tactacgttt cataagtcat
     7081 taatgcgtgt gctctgcgag gctagttttt gtgcaagcac aaaaaatgga ctgaataaat
     7141 cagtccataa gttcaaaacc aaattcaaaa tcaaaaccac aagcaaccaa aaaatgtggt
     7201 tgttatacgt tcataaattt tatgatcact tacgtgtata aaattaaatt cactttcaaa
     7261 atctaaaaac taaatccaat catctaccct atgaattata tcttgaaatt cattcataaa
     7321 tagtgaagca tggtaaccat cacatacaga atgatgaagt tgcagagcaa ctggtatata
     7381 aattttatta ttctcactat aaaatttacc tatcgtaata ataggcaata aaaagctgct
     7441 attgttacca atatttaaat taaatgaact aaaatcaatc caaggaatca ttgaaatcgg
     7501 tatggtgttt tcaggtatcg gttttttagg aaacatttct tctttatctt tatattcaag
     7561 caagtcattt ttataattat tataaaaaga aatgaagttt ttatcagatt cagtccaaat
     7621 gttagtaaat ttttcagttt gcttattaaa aactgtatac aaaggattta acttatccca
     7681 ataacctaat ttattctcac tattaattcc tgttctaaac actttatttt tatttacaac
     7741 ttccataatt gcataaatta aagagggata aatttcatat cctttctttt ttatcatatc
     7801 tttaaacaaa gtaatatcaa tttctttagt aatgctataa gtagtttgct gattaaaata
     7861 gtgttcaaaa tattcttttc tatcccaatt ttctaattca ataatattaa aagtcatata
     7921 taacttcctc ctaaatttta aatttttata tttaggagga ataatcctct gattttttca
     7981 tacgttatgt cacctcgtaa atattaatta tactgaatta gcaattttta tcaaataaaa
     8041 cttattttac ttccaaaacc taaattcacg ttgccaaaaa tcaatctgct tttgcaattg
     8101 tttttcgttc gcttttaaag tcgatttcat taattccgtt aaatcaattg gagatatttc
     8161 tctaatcaat tttttaaatt tagtcttagt attcttactt agctttcccc acatactttc
     8221 ttcatgcaac aaagtataaa ccatagcttg ctcattaatt ttttctaaag tagcccacgc
     8281 aggtttcaag atgtgtaaat cattaaaaca atcattccag taatcaacca tatctctttt
     8341 taattcaact tctacacgcc ataaatgttc agacacaact tcaacatctg cgttatcttt
     8401 acgttcttgt tttttattat aaattctaat aaatctatca ctatcacgga caccaaaata
     8461 ttttgtttct ggcttgccat tacgaccata aaaaacagtt ttcttaactg ctttatcagt
     8521 cattgcatag taatcgctca aatcatcttc aaaatcaaaa gctaagtcta atcttgtaaa
     8581 accgtcatct tccatgtagt cgataatatt ttgttttaac caaatcattt cttcatgtgt
     8641 gagtttattg ggattaaatt caacacgcat attacgtcta tcccaagtat ctgcttttac
     8701 tttgtcatat tcgatataaa ctttttcttg aagtgcctta gctttaaact ttgtttgaag
     8761 tatatcccaa agtcgtattt gtggctctac actcataaag tcagatagct ttttagcatt
     8821 agttttgttc aaatttccaa cgattgtcat ggcatcaaaa cttaatgcgg gttgagattt
     8881 tcccaaagtt tgaccactta accggctatt acttaaccgg ctattagaga cggccggttt
     8941 ttttagtgag atttctgcag ctaatttttc atcaattttc ttcatttttt atcgctccta
     9001 aaataatttt tggaacgaaa aaaggacgag tggaatcgca aaataaattt cgcctgtatc
     9061 cattcgtcca ttttgttttg tattcaaaat caaaaattga tatactaatc atcgaatgaa
     9121 taacaagtgt ggtttgttat tccgggcgag aacgttggta gcgttgctcg tcttttttta
     9181 tgtctttttt tcgttaaaac tattttaaaa ctaaatatat cttaaagcaa ataaattcaa
     9241 ggggctaaaa gcgcccctta aaaacccctt gcctctgaaa agttttgtgt ttcctttccg
     9301 tcggtttcaa aagctaattt actcaaaaaa atagatcaag ggtaaatgca ctcactgcgt
     9361 tcgcccttga gctattttat ttcgcaaatt ttaacgcatt tgccgacgaa aagataaaac
     9421 acataaaaat atacgcagat atatttaaaa cgtcttagaa cgcaaaattt tacgtttaaa
     9481 agctatgcaa ggactgtatg aagcaaaatt attgagttat ccaagaacag atacaccatt
     9541 tattacagag aacgaatttg cttatttaaa agcgaatttt ggcaaatata gcggtttttt
     9601 aggacttgat cttgaaatgg ttcaaacaga gcctagaaag cgttatgtgg acggtagtaa
     9661 ggtacaggaa caccacgcca ttatcccaac aaaacaagta cctaccgaat ctgcattagc
     9721 gaaaatggac gatttacaac gaaaaattta tgctttagtc gttaaaacga ccgttgccat
     9781 gtttttacct gattacttgt atgaagaaac caagatacaa acgaaagtag ctgatttgct
     9841 gtttcaatcg atcggaaaga caccaaagca agaagggtgg aaaattcttt tcaaacaaca
     9901 aaccaaagaa gaaaaagagg acgttcaaac gttaccactc gttatcattg gcgaacgtgc
     9961 cgaagttggt gttaagagtg ttgaaaaaga aacgcaaccg ccaaaagctt ttacagaggg
    10021 tacattatta actgctatga aaacggcgaa taaaacggtt gatgatgaag aagcaatcaa
    10081 gattttacaa gaagttgagg ggattggaac agaagcgaca agagcaagta ttattgaagc
    10141 gttaaaacaa aaagaatata tccaagtgat taagaataag cttgttgtaa ctgaaaaagg
    10201 aaaattattg tgccaggcag ttgaaagtca gcacctttta acgagtgctg aaatgacggc
    10261 taaatgggaa acgtatttaa aaaaaatcgg taaaagagaa ggcaatcaag agaactttat
    10321 tacgaatatc aaaaaattca ttgttcattt actggaagct gtacctaacg atatagaaaa
    10381 actaaatttt tctgattacc aggaacagaa agaaaaagaa gcagaaaaaa gtattgtagg
    10441 aaaatgtcct aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa
    10501 ttatcctgaa tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac
    10561 gaatgtaaaa gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga
    10621 gaaaaagccc tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc
    10681 tttttcaaaa taaacataaa agccctttaa tgatgtgcac cccaaaagtt agactagaaa
    10741 tctaatta
//
LOCUS       DAZUMV010000327         6877 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.130, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000327 DAZUMV010000000
VERSION     DAZUMV010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.130"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1103
                     /locus_tag="ACG8FK_003006"
     CDS             207..1103
                     /locus_tag="ACG8FK_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5098505.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(1246..2187)
                     /locus_tag="ACG8FK_003007"
     CDS             complement(1246..2187)
                     /locus_tag="ACG8FK_003007"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5098506.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2313..3434
                     /locus_tag="ACG8FK_003008"
     CDS             2313..3434
                     /locus_tag="ACG8FK_003008"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5098507.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3692..5194
                     /gene="eat(A)"
                     /locus_tag="ACG8FK_003009"
     CDS             3692..5194
                     /gene="eat(A)"
                     /locus_tag="ACG8FK_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5098508.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            5261..5788
                     /locus_tag="ACG8FK_003010"
     CDS             5261..5788
                     /locus_tag="ACG8FK_003010"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5098509.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            6000..>6877
                     /locus_tag="ACG8FK_003011"
     CDS             6000..>6877
                     /locus_tag="ACG8FK_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5098510.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKG"
BASE COUNT         2264 a         1203 c         1454 g         1956 t
ORIGIN      
        1 ataatgacta atgataacaa attttcagat aattacaact attcagaaaa cttttgggca
       61 tattttaatg gaagaatgat ttatttcatc gtattttcat agaaatgtta aatttttata
      121 aaaataatag gaagcgtttt actggctttt tactatttaa acaggctatc ataggaaaaa
      181 gtgacagaga aagtgaggcg gaacagatga acaaaaacga cgtattgcta attgcgaaaa
      241 ataaatcact ttattttttt ccttccatcc gctctttaga tgtttggtcc ggaaaagatt
      301 tactacagtc ggaattcatt aaatcagctc gacaacagtt ggcaaattat cagctgatcg
      361 tttttttaga ttatggattt gatatggaga tggctcgacg cgtgcgtcca tttaccaatg
      421 caaaaatcgt gttatttttt tggaaccact ttaaagcaga acacgaggcg atgctgaagc
      481 aggcacaaga tgaaccggca atcgatgaga tttatcattt tgatttttta gaagcgaaag
      541 aacttggact aaaacataat agttcctttt attcgaaaca tatggatttt tcttctagcg
      601 aaccaaagac ggatctattt tttggtgcaa caaataacgg cagaaaagaa cgggcagaaa
      661 aatataagca ggaatttaca aaaagaggtg ttacgaccaa ttactttatt cttccgcttc
      721 gaggaaatga acaacctgga taccttactt atgaagaata tctgaaaaaa accggggaat
      781 cgagggggat tctagaactg ttgcgggaag ggcagcaagg agtgactttg cgaacgtttg
      841 aatctatgta ttttcaaaag aaactagtga cagataatca agccatagct cattatcagt
      901 tttatcattc tgaaaatatt tttttgcttc aagaacgtga tttagatgaa ttaccggaat
      961 ttttgcatac gccatttcaa ccaatcccat cagaactgtt ggaatttttt gatgcagaaa
     1021 attgggcaaa acgttttttc actactgact ccgctattta tgaacaatat gaatacgatc
     1081 tttcctctgt taaaaataaa taaaagtgcc aaccaatcat agatgaaaat aaacaagcgc
     1141 aattcttatg gcaacatgag aattgtgttt ttctttttga actttcatgg gggtaaaaaa
     1201 atgagaacca gctgctgatc cagctgattc tcatcagaga gccttttatt ctgcgccaag
     1261 gattccttct tttgcatgga ctaattgggt caaaaaggca caatatggtg tagcaatatc
     1321 atatttttgt ccttttcgcc aaacggcacc attgatgtag tcgatttcag tcaaacgatg
     1381 gtttttgatc aggtcttggt acatagatgg gtaatgcaac ccgatgttgt caggatcata
     1441 agtagaagca atatgttggt acacttcttc ttgatctaat gtgattcctt cttttgctgc
     1501 gatagaagca aactcagata caatcgttcg aaccatgccc tctgccgctt tttgcgcacc
     1561 taattcagca atattacaat ccaatatcgt gcataatcca tttaatgttc cgtttacaca
     1621 agcttttcgc caaatggaat aacgaacatt atgactatag attggattca attttgcttc
     1681 ttgaaagact tctacaactt tttttgtaaa tgcttcgcct tctggttcca agttctctag
     1741 ttcgatttct ccatcaccta ataatgtgac ttttccggga ccagctagac cagctgtcca
     1801 catcgtgatc ccaaataaga tatttttctt tggtacatat ttttccagta catcttcatg
     1861 acctaatcca ttcagtaaac acaaaacata agtattctca gtaataatgg attggatcga
     1921 acgaaacata tcatctaatt gattcgcttt tgtcaaggct acgatcaaat ccacgtgttt
     1981 gttgctttcg atgatttctt ctggtgaata gattggcagt tttgcaacta cttctttgcc
     2041 attaaagtca gcaatcaatc catttgtacg aatggcttca atgtgagcag gccagcgatc
     2101 gatcaagacg acctcatttc cgctttgatg caacattaat ccgattcggc tgcccattgc
     2161 tccggctcca gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt
     2221 ttagtatata gaaaaacaga agaaaatagc attgttttgt ttgaaaaaag tgaaaaaaag
     2281 tgtcttactt aagaagaaag tgagggatat agatggatat tttggcggca atcgattcat
     2341 ttaaaggaag tgcttcttca tttgaattaa atcaggcagt tctaaatggt tgggaagacc
     2401 gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa
     2461 tctattacgc gctcggggga acctggcgta cgattgatgg gatggatctc ttatttcgtc
     2521 agcgaaaaat cagttattta ttgacgaact ggcaaggaaa aaataccgca gtcattgaat
     2581 ctgcacaagt attaggatta gatttacttg ataaaccaac ggatcaaacc atccgacttg
     2641 cttctagtta tgccttggga gagcttatca aagatacact gcttcaagaa gtggaacaaa
     2701 tcattgtcac tttaggagga agcggatgta gcgatggtgg tctaggatta ctccaatctt
     2761 tgggtgcttc tttagaaggg tacacagaag gaaatccttt gttatctact agaaaacttc
     2821 atttggatcg aaggaacgtt cttgtttcta aagtaaaagt gatcatcgct gcagacgtga
     2881 ccaacccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa
     2941 cggatacgac gttagctttc ttggataaac aagctgaaaa gattgctcag cagataaaag
     3001 atgtttggga ggtggattta gatgacatcc caggaagcgg agcagcaggc ggtattggcg
     3061 gagccctttt tttgttagga gcgaagatgg tctctggttt tgagttggtc agccaaatca
     3121 ctggattaga aaaacagatc gaacaagcgg atgtcatcat tacaggagaa gggcggattg
     3181 atcagcaaac aatccacgga aaagttccat atggcgtagc agtgcttgca aaaaaatatc
     3241 gaaaaccaat aatcgctatt tgcggaagta gagaccgaga gctggaagga ctctttgatt
     3301 ttctccccat tgtcttaagc attcaattag gaccaatcga tttagaggaa gcaatggaac
     3361 acaggcaaac attagaaaaa acagaaatct taggacagac actcagcaga tttttcaaca
     3421 ttctcccgaa gtagttgaca aaaacaattt ctttatgcta tcgtaatttc aaatatagga
     3481 caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact
     3541 gtaccttgtg gagacaaggg agcagtgtgc agattttcgg gattggcttg gttctttcga
     3601 tgtatgggtc tttttgtgtg ccccaaccct tgcatcctct ttagccagtt ctgaaaatag
     3661 gactggtctt tttttacgag gtgatggaat aatgtctaaa atcgaaataa aaaatctgac
     3721 attcggctat gacagccaag gcacattatt atttgaacaa gccaatctaa actttgacac
     3781 acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat
     3841 cctgcaaaac aaactccctt atcaagggca agtgatccat cagcaagcgt ttgtctattt
     3901 cccgcagcaa acaaaagata aagagcgttt gacctattat gtgttaaatg agattatgga
     3961 ttttgagata tgggaaattg aaagagagct ccaactgatg caaacagatc ctgaaatctt
     4021 atggagagaa ttcagcacac tatcaggagg agagaagaca aaagtcctac tggcactctt
     4081 atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc
     4141 tggcagaaaa caagtagcgg cttacttgaa aaagaaaaaa caaggattca tcgtggtgag
     4201 tcatgatcgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaagagcca
     4261 actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga
     4321 atttgaaata gctaaaaatg aaaaattgaa aaaagaagtc agcaggctaa agaaaacagc
     4381 agctgaaaaa gcagaatggt ctcgttcccg agaaggggac aaaacaaaga aaaaagtcgg
     4441 attcatcgat acggaatcta ggcgagtgaa taaaggagca gtaggcgctg atgctgcacg
     4501 gacgatgaaa cgatctaaag caatcgtgaa tcggatggaa acccagatca gtgagaaaga
     4561 aaaattatta aaagatatcg agtatatcga tgctttgacg atgaatagcc aagtgtctca
     4621 tcataaacgg ctgttaagcg tagaaaatct tcaattaggg tatgaaaatc tgttattcga
     4681 gccagttcat tttacgatcg aacctcatca gcggatggca atttcaggtc ctaacggcgc
     4741 gggaaagtca tccattatcc attatcttct ggggacattc aacggcaagg ttgtaggaga
     4801 aaaatcccag cccaaacatt tgaccattag ttacgtacgc caaaattatg aagacaatcg
     4861 agggacattg gcggaattcg cgaagaaaaa ccgagtagac tatcaagcat ttttgaataa
     4921 tctccgaaag cttgggatgg aaagagatgt ttttcataat aaaatcgaac agatgagtat
     4981 gggacaacgg aaaaaagtgg aattggctaa atctttatcc caaccagctg agctctatat
     5041 atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat
     5101 tttgaacgtg aaacctgcta tgttactagt ggaacatgat caaacctttc tagataaagt
     5161 atctactgag attatttctc ttgagaaaat ctaaaccttg taatctcaaa gacaacaagg
     5221 tatggttaag gagaactttg tcaacgaagg agagaatcac atgctttatg taacgagaca
     5281 cggagaaaca acttggaacg cacaaggatt agtttgcggg cgagcagatg tgccgctgac
     5341 tgaaaaagga cagatgcagg cacaggcact agcagaaaag gtagtagatt tgcctgttcc
     5401 aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac aagcagtagt
     5461 tgatcgtctc aatttgccga tgaccgctga tgagcgattg gtagaaatgg attttgggga
     5521 ttatgacgga ctgccaagca aagatgaaaa ctttcaaaaa gcgcgtttag cgtttgctgt
     5581 acgtttccca aatggagagt cggtcttaga tgtgtacgca cggatcgttc ctttattgaa
     5641 agaatgcatg gaagacgaag aaaatgtcta cttgttagtc tgtcataatg ccttgataag
     5701 agtgatcaat gcttattttc atccaatgcc taatgaaggg ttctttacat tcatggtaga
     5761 caatacagaa ttaatttctt atgagtaatt agacttttgt gttatttaaa taaaatgttt
     5821 caaggcactg cttttgctta gcagtgcctt ttttgtggct gtgtcaattc ttttggcatt
     5881 ataaatccaa tttttagaaa taaaatttaa ttattttaac aaaagtattg aaattttgtt
     5941 tctttagata tatactctag tcatagaaat gaagacggat acagaaaaag agggataata
     6001 tgtttggctt ttcaatattt atgaatgatt ctttgtcaga agagaagaaa cagtatatcc
     6061 gccaaatggc atccaatggc tttgtcggta ttttcacttc catgcatata ccggaagatg
     6121 atgtttcggc ttatcaaaat cgattgatcg aactaggaag cgaagcgaaa aagaataaac
     6181 tagagttgat ggtcgatatt tcaggagagg cattgaaacg agctggcttt tcattagaag
     6241 atctaacacc cttgaaagca attggtgtaa cagggttaag aatggattat catatttcta
     6301 atcagcagat cgccgactgg tcacatcaat tgaaaatcag tttgaatgcc agcacgatca
     6361 cgcctaatga tatcgatgag ttgaaagaag cagcggctga tttctcacag attgaagctt
     6421 ggcataatta ttatccacgt cttgaaacag ggttggacaa agaatggtat caggaaaaaa
     6481 atcaatggct caaaactcaa ggattccttg tacaaggctt tgtaccagga gatacagagt
     6541 tgagaggtcc actgtatcaa ggattaccaa ctttggaaga acatcgaggg atccatcctc
     6601 tggcaggtgc tcttgatcta ttagcaagta acacggatat tgtttatatc ggagatgcag
     6661 gtctgtcaga acacgttcag gagcaatttg cttctttcca aaaagaacag acagtcttgc
     6721 tccatacgga acctgtagat gaagtttttt acgaatatat attaggaaaa catacgaatc
     6781 ggcaagatga tgcaagagat gtcatacgaa gcgcagatgc gcgttttcga gagattccgc
     6841 caatcccagt aagaaataca gctaagcgta tgaaagg
//
LOCUS       DAZUMV010000328         6642 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.132, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000328 DAZUMV010000000
VERSION     DAZUMV010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.132"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..209
                     /locus_tag="ACG8FK_003012"
                     /pseudo
     CDS             1..209
                     /locus_tag="ACG8FK_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            447..683
                     /locus_tag="ACG8FK_003013"
     CDS             447..683
                     /locus_tag="ACG8FK_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098511.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="ACG8FK_003014"
     CDS             690..2642
                     /locus_tag="ACG8FK_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5098512.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="ACG8FK_003015"
     CDS             complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="ACG8FK_003015"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5098513.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3486..4613
                     /locus_tag="ACG8FK_003016"
                     /pseudo
     CDS             3486..4613
                     /locus_tag="ACG8FK_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
     gene            4711..6084
                     /gene="tet(L)"
                     /locus_tag="ACG8FK_003017"
     CDS             4711..6084
                     /gene="tet(L)"
                     /locus_tag="ACG8FK_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS5098514.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         2198 a         1055 c         1242 g         2147 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     3181 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     3241 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     3301 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     3361 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     3421 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     3481 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     3541 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     3601 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     3661 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     3721 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     3781 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     3841 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3901 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3961 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     4021 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     4081 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     4141 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     4201 atggcttata ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg
     4261 tattggaaca agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta
     4321 aaatttatgc gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg
     4381 cgaacatcgt agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg
     4441 ctcggtgtat tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt
     4501 gtttaacaga gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc
     4561 gtccaaatag tcggatagat aaagtacgat atatgttcaa taaaataact tagtgtattt
     4621 tatgttgtta tataaatatg gtttcttgtt aaataagatg aaatattttt taataaagat
     4681 ttgaattaaa gtgtaaagga ggaattaatt atgaagtgta atgaatgtaa cagggttaaa
     4741 tacaaccaaa ttttaatttg gctttgcgtt ttatcttttt ttagcgtatt aaatgaaatg
     4801 gttttgaacg tctcattacc tgatattgca aatgatttta ataaaccacc tgcgagtaca
     4861 aactgggtga acacagcctt tatgttaacc ttttccattg gaacagctgt atatggaaag
     4921 ctatctgatc aattaggcat caaaaggtta ctcctatttg gaattataat aaattgtttc
     4981 gggtcggtaa ttgggtttgt tggccattct ttcttttcct tacttattat ggctcgtttt
     5041 attcaagggg ctggtgcagc tgcatttcca gcactcgtaa tggttgtagt tgcgcgctat
     5101 attccaaagg aaaatagggg taaagcattt ggtcttattg gatcgatagt agccatggga
     5161 gaaggagtcg gtccagcgat tggtggaatg atagcccatt atattcattg gtcctatctt
     5221 ctactcattc ctatgataac aattatcact gttccgtttc ttatgaaatt attaaagaaa
     5281 gaagtaagga taaaaggtca ttttgatatc aaaggaatta tactaatgtc tgtaggcatt
     5341 gtatttttta tgttgtttac aacatcatat agcatttctt ttcttatcgt tagcgtgctg
     5401 tcattcctga tatttgtaaa acatatcaag aaagtaacag atccttttgt tgatcccgga
     5461 ttagggaaaa atataccttt tatgattgga gttctttgtg ggggaattat atttggaaca
     5521 gtagcagggt ttgtctctat ggttccttat atgatgaaag atgttcacca gctaagtact
     5581 gccgaaatcg gaagtgtaat tattttccct ggaacaatga gtgtcattat tttcggctac
     5641 attggtggga tacttgttga tagaagaggt cctttatacg tgttaaacat cggagttaca
     5701 tttctttctg ttagcttttt aactgcttcc tttcttttag aaacaacatc atggttcatg
     5761 acaattataa tcgtatttgt tttaggtggg ctttcgttca ccaaaacagt tatatcaaca
     5821 attgtttcaa gtagcttgaa acagcaggaa gctggtgctg gaatgagttt gcttaacttt
     5881 accagctttt tatcagaggg aacaggtatt gcaattgtag gtggtttatt atccataccc
     5941 ttacttgatc aaaggttgtt acctatggaa gttgatcagt caacttatct gtatagtaat
     6001 ttgttattac ttttttcagg aatcattgtc attagttggc tggttacctt gaatgtatat
     6061 aaacattctc aaagggattt ctaaatcgtt aagggatcaa ctttgggaga gagttcaaaa
     6121 ttgatccttt ttttataaca ggaattcaaa tctttttgtt ccattaaagg gcgcgattgc
     6181 tgaataaaag atacgagaga cctctcttgt atctttttta ttttgagtgg ttttgtccgt
     6241 tacactagaa aaccgaaaga caataaaaat tttattcttg ctgagtctgg ctttcggtaa
     6301 gctagacaaa acggacaaaa taaaaattgg caagggttta aaggtggaga ttttttgagt
     6361 gatcttctca aaaaatacta cctgtccctt gctgattttt aaacgagcac gagagcaaaa
     6421 cccccctttg ctgaggtggc agagggcagg tttttttgtt tcttttttct cgtaaaaaaa
     6481 agaaaggtct taaaggtttt atggttttgg tcggcactgc cgacagcctc gcagagcaca
     6541 cactttatga atataaagta taatgtgtta tactttactt ggaagtggtt gccggaaaga
     6601 gcgaaaatgc ctcacatttg tgccacctaa aaaggagcga tt
//
LOCUS       DAZUMV010000329         5463 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.152, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000329 DAZUMV010000000
VERSION     DAZUMV010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.152"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..446
                     /locus_tag="ACG8FK_003018"
     CDS             <1..446
                     /locus_tag="ACG8FK_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5098515.1"
                     /translation="HLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEENPLLQSS
                     LQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKHCEMDHKD
                     TGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            430..1059
                     /locus_tag="ACG8FK_003019"
     CDS             430..1059
                     /locus_tag="ACG8FK_003019"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5098516.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1413..2891
                     /gene="msr(C)"
                     /locus_tag="ACG8FK_003020"
     CDS             1413..2891
                     /gene="msr(C)"
                     /locus_tag="ACG8FK_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5098517.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2938..3384
                     /locus_tag="ACG8FK_003021"
     CDS             2938..3384
                     /locus_tag="ACG8FK_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5098518.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3583..4098)
                     /locus_tag="ACG8FK_003022"
     CDS             complement(3583..4098)
                     /locus_tag="ACG8FK_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5098519.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4215..5417
                     /locus_tag="ACG8FK_003023"
     CDS             4215..5417
                     /locus_tag="ACG8FK_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5098520.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1704 a          918 c         1176 g         1665 t
ORIGIN      
        1 gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc tttgaagaaa
       61 atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa gaaaatccat
      121 tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt actcgtcatc
      181 tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag aacaaccggg
      241 aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt gaaatggacc
      301 ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg aaacaatttc
      361 taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa acgaaagagg
      421 ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag aagatccgtt
      481 cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat gatcaaagaa
      541 gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt cgctgaaagc
      601 gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca aaatgatact
      661 tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc
      721 tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca acaattagct
      781 gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt agtcaaagtc
      841 gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca
      901 aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt ggataaagac
      961 cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg ttacgagtct
     1021 gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag ttactattca
     1081 gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa
     1141 tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata aattcaaaag
     1201 acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac gacagtagaa
     1261 taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc agagtaggaa
     1321 aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt atcgataatt
     1381 gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta aatataacaa
     1441 atctacaagt cagctttggc aaccagctag aactatctat tgattctctt cgtgtctatc
     1501 agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact ttgctcaaac
     1561 taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc accttcaact
     1621 acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa ttagccagtc
     1681 atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg gaagaacgaa
     1741 agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta gacgagccaa
     1801 caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt tattattatg
     1861 gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca tcgaaaatct
     1921 gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc tatctcactc
     1981 aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg aaagagaaaa
     2041 aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt
     2101 ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct aaacaaaaag
     2161 acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa
     2221 taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta ccaaaatctc
     2281 ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa agaagtggga
     2341 gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc gcgattgtcg
     2401 gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa ggagaaggaa
     2461 tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat caaatgacat
     2521 ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc
     2581 gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg ttatgtacat
     2641 taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag ccaagtaatg
     2701 tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa gctttagaga
     2761 agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct tactttgtcg
     2821 agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa gtacttacga
     2881 gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg gtacagattg
     2941 cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt gatggtcttg
     3001 gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag tttgttgatt
     3061 ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat cggtgtgctg
     3121 gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca aatgatctta
     3181 actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt cggtgtgatc
     3241 atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg gaaaatgaat
     3301 gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag aagtgtgcta
     3361 aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc cttggcatgc
     3421 tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac gataggtttt
     3481 ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat tgaagataat
     3541 ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa aaaactgctg
     3601 catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca taattctgcg
     3661 ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt cattcaattt
     3721 tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata gcagtccagt
     3781 agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt catctttttg
     3841 cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc cttcatcagt
     3901 caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca cataaccctg
     3961 ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc aaaaaattgt
     4021 gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc ataataatat
     4081 ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat tatatcacag
     4141 taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg tatgatgcta
     4201 atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa caaacaaatg
     4261 aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg atcgctttta
     4321 tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta aacgctacgc
     4381 cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg gtgatgctat
     4441 ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca ggattgttta
     4501 tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg ctgattgggt
     4561 tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta gcaacgatcg
     4621 tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca gcacttggct
     4681 gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca tggcgttatc
     4741 cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg gtacttttac
     4801 ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa gcgttaagca
     4861 atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga ttctttactt
     4921 tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc ggttttgtat
     4981 tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg gctcttgagc
     5041 ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt atttgtttgc
     5101 tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt gtgttagcag
     5161 gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa ataccaggtc
     5221 tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt ggagctttag
     5281 caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg ttttattttg
     5341 caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat tattttgcta
     5401 caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa
     5461 gaa
//
LOCUS       DAZUMV010000330         4659 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.173, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000330 DAZUMV010000000
VERSION     DAZUMV010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.173"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..209
                     /locus_tag="ACG8FK_003024"
                     /pseudo
     CDS             1..209
                     /locus_tag="ACG8FK_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            447..683
                     /locus_tag="ACG8FK_003025"
     CDS             447..683
                     /locus_tag="ACG8FK_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098521.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="ACG8FK_003026"
     CDS             690..2642
                     /locus_tag="ACG8FK_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5098522.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="ACG8FK_003027"
     CDS             complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="ACG8FK_003027"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5098523.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3486..4613
                     /locus_tag="ACG8FK_003028"
                     /pseudo
     CDS             3486..4613
                     /locus_tag="ACG8FK_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
BASE COUNT         1624 a          751 c          836 g         1448 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     3181 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     3241 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     3301 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     3361 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     3421 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     3481 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     3541 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     3601 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     3661 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     3721 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     3781 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     3841 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3901 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3961 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     4021 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     4081 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     4141 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     4201 acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg
     4261 tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta
     4321 aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg
     4381 caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg
     4441 ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt
     4501 gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc
     4561 gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt
     4621 tatgttgtta tataaatatg gtttcttatt aaataagat
//
LOCUS       DAZUMV010000331         3970 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.181, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000331 DAZUMV010000000
VERSION     DAZUMV010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.181"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8FK_003029"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8FK_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS5098524.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG8FK_003030"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG8FK_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS5098525.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1256 a          613 c          816 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaagaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata atgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DAZUMV010000332         3970 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.182, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000332 DAZUMV010000000
VERSION     DAZUMV010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.182"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8FK_003031"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8FK_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS5098526.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG8FK_003032"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG8FK_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS5098527.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1255 a          613 c          817 g         1285 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaagaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca
     3661 agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc
     3721 tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt
     3781 tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc
     3841 ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata atgtgttata
     3901 ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa
     3961 aggagcgatt
//
LOCUS       DAZUMV010000333         3685 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.190, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000333 DAZUMV010000000
VERSION     DAZUMV010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.190"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..229
                     /locus_tag="ACG8FK_003033"
     CDS             <1..229
                     /locus_tag="ACG8FK_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098528.1"
                     /translation="DFILYLKLCNRTLLVEDASLSGENERINELIEFIRKNKFRTLEY
                     DDKLVRKIIQSVTVYEGHFVIAFKSGIEMEI"
     gene            372..596
                     /locus_tag="ACG8FK_003034"
     CDS             372..596
                     /locus_tag="ACG8FK_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5098529.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            611..1480
                     /locus_tag="ACG8FK_003035"
     CDS             611..1480
                     /locus_tag="ACG8FK_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFS5098530.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1461..2195
                     /locus_tag="ACG8FK_003036"
     CDS             1461..2195
                     /locus_tag="ACG8FK_003036"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5098531.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2228..3136
                     /locus_tag="ACG8FK_003037"
     CDS             2228..3136
                     /locus_tag="ACG8FK_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFS5098532.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3133..3613
                     /locus_tag="ACG8FK_003038"
                     /pseudo
     CDS             3133..3613
                     /locus_tag="ACG8FK_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1279 a          533 c          800 g         1073 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccctt ttagtagaag atgcctccct
       61 cagtggcgag aacgagcgaa tcaatgagct gattgaattt atccgcaaga acaaattccg
      121 caccttagaa tatgatgata agcttgtaag gaagataatc cagagcgtta cagtctatga
      181 aggccacttc gtcatagcct tcaaatctgg catcgaaatg gaaatatgaa aaccagaagt
      241 aaatagccca tgactctaca gtagagttgt gggttttctt ttgcttgctg attataataa
      301 tattttaata actattgtgt ttatcaacca aatggtttat aatattctta gcgtaaactt
      361 ggaggtgctg tatgactacg gcagaaatga ttaaagaact gtgtgagcaa atgaatataa
      421 gtgtttccga acttgctaga cgtattggcc agactccaca gaatttcaat aaaaaattac
      481 aacgagaaac agtaaccttg gatgagttga aggccattgc tgatgtgcta ggtgtcaagt
      541 ttgtgcaggc atttatttta ccagatggag aagaaataaa aatatctaac gaataaagga
      601 ggatgtttat atggtaggta atgttattca aatagtaaca gagaaattat catctttgcc
      661 ttttatagaa ggcatcgtat tagggggctc acgtgcaaga ggcacccata cagaaaattc
      721 tgatatagat atcggaatct attacaattc agactcattt gacctgacag cgattaatca
      781 aattgctaca gaattggatg ataaaaatag aaacaacctt gttgtgcctc ccggagcgtg
      841 gggtgattgg attaatggcg gcggatggtt atttataaat gggtatcatg ttgacttgat
      901 tttacgtgat ataaaacggg tagaacaaat aatcaaagat acggagcaag gaattgttac
      961 tgccaattat cagaccgggc atccccatgg ttatataagt gcaatgtatc gtggagaatt
     1021 agcgattagt aaaatactat atgctaagaa cgaaagctta tgcgaattaa aaaagcaggc
     1081 agaaatttac cctactgctc taaagaaaag cttgatgaac ttttttatat ttgaagctga
     1141 attctcttta atgtttgtaa aagcaaatgc gggggtagag gataaatatt atattgcagg
     1201 ccatgttttt cgtattattt catgcttaaa tcaagtacta tttgcatgta ataatgctta
     1261 ttgcattaac gaaaagaaag ctataaaact gcttgaaact tttgaatata aacctgaaaa
     1321 atatgccgaa agagttaatc atatttttga agtactcggt ctttctcttt ttgaatgcta
     1381 cgatatgacc gagaaacttt ataaagaagt gaatgaaatt gtatcggaga taaataactt
     1441 tttaaacgag gagagttcag atgaaagaaa acaaatatga tgataatata ttttttcaaa
     1501 aatacagtca aatgagtcgc tcgcagaaag gactggctgg tgcgggagaa tgggagactt
     1561 tgaaaaagat gctacctgat tttaagggta agcgtgtgct tgatttagga tgcggctatg
     1621 gatggcactg tatatatgcg atggaaaacg gtgcttcctc tgtagtaggt gttgatattt
     1681 ctcataaaat gctcgaagta gcaaaaggaa aaacccattt tccacagatt gaatatgaat
     1741 gctgtgccat agaagatgtg gatttcccag aggagagctt tgatgtaata ctaagttcgc
     1801 ttgcgtttca ttatgtagca gactatgaga atttaataaa aaagatatat aggatgctga
     1861 aggctggtgg caatttagtt tttacagttg aacatcctgt ttttactgct catggaacac
     1921 aagactggta ttataacgaa aaaggagaaa tactgcattt cccggtggac aattattatt
     1981 atgagggcaa acggacagct atgtttttgg aagaaaaggt tacaaaatat catagaacac
     2041 tgaccacata tctaaataca ctgctttcaa atagttttat aataaatcag attgtggagc
     2101 cacagccgcc agagaacatg atggatattc cggggatggc ggatgaaatg cgacgcccaa
     2161 tgatgctgat tgtatcggca aaaaagaaga tgtaataata tagaaaaaat aaacgaggag
     2221 tatgtaaatg agatcagaaa aagaaatgat ggatttagta ctttctttag cagaacagga
     2281 tgaacgtatt cgaattgtga cccttgaggg gtcacgcgca aatattaata tacctaaaga
     2341 tgaatttcag gattatgata ttacatattt tgtaagtgat atagaaccgt ttatatctaa
     2401 tgatgactgg cttaatcaat ttgggaatat aataatgatg caaaagccgg aggatatgga
     2461 attattccca cctgaagaaa agggattttc ctatcttatg ctatttgatg attacaataa
     2521 aattgatctt accttattgc ccttggaaga gttagataat tacctaaagg gcgataaatt
     2581 aataaaggtt ctaattgata aagattgtag aattaaaagg gacatagttc cgactgatat
     2641 agattatcat gtaagaaagc caagcgcaag ggagtatgat gattgctgca atgaattttg
     2701 gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag gaaattttat ttgctattga
     2761 tcattttaat cagattgttc gccatgagct gctgagaatg atatcatgga aggtcggcat
     2821 cgaaacaggc tttaaattaa gtgtaggcaa gaactataag tttattgaaa ggtatatatc
     2881 cgaggatttg tgggagaaac ttttgtccac ctaccggatg gattcctatg aaaacatatg
     2941 ggaagcatta tttctatgcc atcaattgtt cagggcggta tccggtgagg tggcggaaag
     3001 gcttcattat gcctatccgg agtatgatag gaatataaca aaatatacca gggacatgta
     3061 taaaaaatac actggtaaaa ccggctgcct ggatagcaca tatgccgctg atatagaaga
     3121 gaggcgggaa cagtgattac agaaatgaaa gcagggcacc tgaaagatat cgataaaccc
     3181 agcgaaccat ttgaggtgat aggtaagatt ataccgaggt atgaaaacga gaattggacc
     3241 tttacagaat tactctatga agcgccatat ttaaaaagct accaagacga agaggatgaa
     3301 gaggatgagg aggcagattg ccttgaatat attgacaata ctgataagat aatatatctt
     3361 ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag gcagcgcgct
     3421 tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa tgcttgaaac
     3481 ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca aaatcggctc
     3541 cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta
     3601 tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgtggt tctgttgcaa
     3661 agttttaaat aaagaataaa atccc
//
LOCUS       DAZUMV010000334         2750 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.211, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000334 DAZUMV010000000
VERSION     DAZUMV010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.211"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            81..1049
                     /locus_tag="ACG8FK_003039"
     CDS             81..1049
                     /locus_tag="ACG8FK_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS5098533.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1042..2073
                     /gene="vanA"
                     /locus_tag="ACG8FK_003040"
     CDS             1042..2073
                     /gene="vanA"
                     /locus_tag="ACG8FK_003040"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS5098534.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2079..2687
                     /locus_tag="ACG8FK_003041"
     CDS             2079..2687
                     /locus_tag="ACG8FK_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFS5098535.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          812 a          532 c          679 g          727 t
ORIGIN      
        1 ttcagagaaa gatttgacaa gattaccatt ggcatccccg ttttatttgg tgcctttcac
       61 agaaagggtt ggtcttaatt atgaataaca tcggcattac tgtttatgga tgtgagcagg
      121 atgaggcaga tgcattccat gctctttcgc ctcgctttgg cgttatggca acgataatta
      181 acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt caatcaatgt atcagtgtgg
      241 gacataaatc agagatttcc gcctctattc ttcttgcgct gaagagagcc ggtgtgaaat
      301 atatttctac ccgaagcatc ggctgcaatc atatagatac aactgctgct aagagaatgg
      361 gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt tgccgattat actatgatgc
      421 taattcttat ggcagtacgc aacgtaaaat cgattgtgcg ctctgtggaa aaacatgatt
      481 tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat gacagttggt gtggtgggaa
      541 cgggccagat aggcaaagcg gttattgagc ggctgcgagg atttggatgt aaagtgttgg
      601 cttatagtcg cagccgaagt atagaggtaa actatgtacc gtttgatgag ttgctgcaaa
      661 atagcgatat cgttacgctt catgtgccgc tcaatacgga tacgcactat attatcagcc
      721 acgaacaaat acagagaatg aagcaaggag catttcttat caatactggg cgcggtccac
      781 ttgtagatac ctatgagttg gttaaagcat tagaaaacgg gaaactgggc ggtgccgcat
      841 tggatgtatt ggaaggagag gaagagtttt tctactctga ttgcacccaa aaaccaattg
      901 ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt gataatcaca ccgcatacgg
      961 cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa aaccattaaa aactgtttgg
     1021 attttgaaag gagacaggag catgaataga ataaaagttg caatactgtt tgggggttgc
     1081 tcagaggagc atgacgtatc ggtaaaatct gcaatagaga tagccgctaa cattaataaa
     1141 gaaaaatacg agccgttata cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa
     1201 aaaccttgcg cggaatggga aaacgacaat tgctattcag ctgtactctc gccggataaa
     1261 aaaatgcacg gattacttgt taaaaagaac catgaatatg aaatcaacca tgttgatgta
     1321 gcattttcag ctttgcatgg caagtcaggt gaagatggat ccatacaagg tctgtttgaa
     1381 ttgtccggta tcccttttgt aggctgcgat attcaaagct cagcaatttg tatggacaaa
     1441 tcgttgacat acatcgttgc gaaaaatgct gggatagcta ctcccgcctt ttgggttatt
     1501 aataaagatg ataggccggt ggcagctacg tttacctatc ctgtttttgt taagccggcg
     1561 cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca
     1621 attgaatcgg caagacaata tgacagcaaa atcttaattg agcaggctgt ttcgggctgt
     1681 gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag ttgttggcga ggtggaccaa
     1741 atcaggctgc agtacggaat ctttcgtatt catcaggaag tcgagccgga aaaaggctct
     1801 gaaaacgcag ttataaccgt tcccgcagac ctttcagcag aggagcgagg acggatacag
     1861 gaaacggcaa aaaaaatata taaagcgctc ggctgtagag gtctagcccg tgtggatatg
     1921 tttttacaag ataacggccg cattgtactg aacgaagtca atactctgcc cggtttcacg
     1981 tcatacagtc gttatccccg tatgatggcc gctgcaggta ttgcacttcc cgaactgatt
     2041 gaccgcttga tcgtattagc gttaaagggg tgataagcat ggaaatagga tttacttttt
     2101 tagatgaaat agtacacggt gttcgttggg acgctaaata tgccacttgg gataatttca
     2161 ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt agggacatac gagttggctg
     2221 aatcgctttt gaaggcaaaa gaactggctg ctacccaagg gtacggattg cttctatggg
     2281 acggttaccg tcctaagcgt gctgtaaact gttttatgca atgggctgca cagccggaaa
     2341 ataacctgac aaaggaaagt tattatccca atattgaccg aactgagatg atttcaaaag
     2401 gatacgtggc ttcaaaatca agccatagcc gcggcagtgc cattgatctt acgctttatc
     2461 gattagacac gggtgagctt gtaccaatgg ggagccgatt tgattttatg gatgaacgct
     2521 ctcatcatgc ggcaaatgga atatcatgca atgaagcgca aaatcgcaga cgtttgcgct
     2581 ccatcatgga aaacagtggg tttgaagcat atagcctcga atggtggcac tatgtattaa
     2641 gagacgaacc ataccccaat agctattttg atttccccgt taaataaact tttaaccgtt
     2701 gcacggacaa acggttctgt tgcaaagttt taaataaaga ataaaatccc
//
LOCUS       DAZUMV010000335         2093 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.231, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000335 DAZUMV010000000
VERSION     DAZUMV010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.231"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..787
                     /gene="vanR-A"
                     /locus_tag="ACG8FK_003042"
     CDS             92..787
                     /gene="vanR-A"
                     /locus_tag="ACG8FK_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS5098536.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="ACG8FK_003043"
     CDS             765..1919
                     /locus_tag="ACG8FK_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS5098537.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          683 a          399 c          448 g          563 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccg
//
LOCUS       DAZUMV010000336         2057 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.234, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000336 DAZUMV010000000
VERSION     DAZUMV010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.234"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..421
                     /locus_tag="ACG8FK_003044"
                     /pseudo
     CDS             72..421
                     /locus_tag="ACG8FK_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(693..983)
                     /locus_tag="ACG8FK_003045"
     CDS             complement(693..983)
                     /locus_tag="ACG8FK_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5098538.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1191..1882)
                     /locus_tag="ACG8FK_003046"
                     /pseudo
     CDS             complement(1191..1882)
                     /locus_tag="ACG8FK_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          578 a          385 c          305 g          789 t
ORIGIN      
        1 tcctaataga gtataacata gctttaagca ttacttaaag ataacttaaa agttatcttt
       61 taaatactta attgttaaat accttatttg atgcaaaaga aatctacgat agtcaacaaa
      121 aaaattttca ttatatccag aaaaattttt cttagttaag gacctatgga ttgcagtaat
      181 gcagaattcg tcgaataaac tgccaaagac atacaatcat attgctttta aaattgacga
      241 acaagatata gattcgtttg tctcaaaaat ccaaatgtta ggtttaactg tcgaaccagg
      301 acgttctcgt gtgaaaggcg aggcttcttc aatttacttt tacgactatg ataatcacct
      361 cttcgagtta cataccggta ctctccagga acgtttaaaa tcctacaatt ccactacctg
      421 agactagtaa tgactagtct ctttttatgg cctctgatcc ccccattgag cgaatactaa
      481 tagggcttcg aattgatctg ttcaatcttt aagcaatatc cctcaaatga gtttggaaag
      541 tttagtgatt catcacaaat acttaaatac atgtagttat atatagattg aggttcttgt
      601 gagcgtatgc tcgtgtgggt tcaaatccca tcgtctgcat ccatctttcc aaaacaagcg
      661 gctaggacat ttttgtctca gccgcttgtt ttctagttta ttctttcatc ttgtcggacc
      721 ttttttcttg ggtatagact tttctcatat tctaccacaa ttttgggttt ccctaacaca
      781 tgataggatc tcacaacaaa ttgctttcta ttattataat aacctgtaac gactaacttc
      841 atcccttcgt ctacttcagc taggaagttc aaactatggg aagcaatcaa acaattgacc
      901 ccatttaaac tgaaacggat caacggtctg gcactaactt gcaaagtacg gatcttatct
      961 actgttccat attttttctc cataaccttc acctcaatac cattatacga acatacattc
     1021 cctttgtaaa gaagaaactc tgttttatct ttttgacttt ttgtctatta cctttcttaa
     1081 aatcatattg gcacttcctg cgaagatgaa ggacatgtac taaaaaaaag aggcagcagt
     1141 atagattact gttgcttctt tactaattta ggtttacttg ttaagaaaga ttatttacgc
     1201 aaatgttctt caacaatttg ctgagattta tcagctgtat cttccacttc atcttttgct
     1261 ttttctaact tttgaatata ttgtttcaag cttgagtttt cagcagttgc ttcgtctaat
     1321 ttcttttgtg tatctgttaa ctgatttcta catttgtttt ttcttgtgtc agtgttgcga
     1381 tttgttgatt caaaccattt acagtatttt gtaattctga aagttttgct tgtgcttctt
     1441 gtagtcttgc tgtccaatct gcattcagat gattgatttc atcatttttt tggttgatct
     1501 catctagttt gacattgatt tcattttgtt tgctttgtag ttgtgcatcc ttgctgacta
     1561 attgactttc taagttttgt ttttctgctg ccgattgatt tttttggttt tctaattcat
     1621 ttacttttgt ttggtattct tggacatctg cctttgaatc ttgcaatgtc tgttcttttt
     1681 gtgctagtcg agcggtaaga tctgtaatcg cttgctcttt atctgtcaat gtatgatcaa
     1741 gctgtgtcag attctcattg attgtatcaa tatctgtctc tccaccccac aaaataattt
     1801 catttgcgta ccgcgtgcct gtgtagccaa ttagacatag aaatgcaacg acaatcccac
     1861 cagtaataaa tttctttttc atttgcatag ctccttaatc caatactttt acatgctaaa
     1921 agtaagtgat ctcaaacaca atgtcaagcg tttttaaatt acatatgtaa ttttaatata
     1981 aatactatca aatactcatt taatcctcct tttttatcta tttaaaaaat cttttttata
     2041 gagaaaaatg caggtta
//
LOCUS       DAZUMV010000337         1987 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.239, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000337 DAZUMV010000000
VERSION     DAZUMV010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.239"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8FK_003047"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8FK_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS5098539.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          682 a          309 c          410 g          586 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagat
//
LOCUS       DAZUMV010000338         1987 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.240, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000338 DAZUMV010000000
VERSION     DAZUMV010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.240"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8FK_003048"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8FK_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS5098540.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          681 a          309 c          411 g          586 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagat
//
LOCUS       DAZUMV010000339         1229 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.270, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000339 DAZUMV010000000
VERSION     DAZUMV010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.270"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1103
                     /locus_tag="ACG8FK_003049"
                     /note="aph(2'')-Ia"
     CDS             <1..1103
                     /locus_tag="ACG8FK_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS5098541.1"
                     /translation="TRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIE
                     DLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSV
                     AYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILG
                     YKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEE
                     YILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLT
                     GIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEY
                     YPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          529 a           95 c          201 g          404 t
ORIGIN      
        1 gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag
       61 ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt
      121 ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt
      181 atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa
      241 ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg
      301 atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata
      361 agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa
      421 ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag
      481 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
      541 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      601 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      661 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      721 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      781 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      841 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      901 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      961 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
     1021 ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt
     1081 ataaaaggac ttataaagat tgattatata atatatgaaa agctattata aaagacatta
     1141 gtattaaata gtttaaaaaa atgaaaaata ataaaggaag tgagtcaatt ccagactcct
     1201 gtgtaaaatg ctatacaatg tttttacca
//
LOCUS       DAZUMV010000340         1124 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.275, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000340 DAZUMV010000000
VERSION     DAZUMV010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.275"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..416
                     /locus_tag="ACG8FK_003050"
     CDS             24..416
                     /locus_tag="ACG8FK_003050"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5098542.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1124
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8FK_003051"
     CDS             417..>1124
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8FK_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HFS5098543.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKG"
BASE COUNT          456 a           88 c          195 g          385 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggt
//
LOCUS       DAZUMV010000341          902 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5013
            SAMEA115707440-rid22576513.guided.287, whole genome shotgun
            sequence.
ACCESSION   DAZUMV010000341 DAZUMV010000000
VERSION     DAZUMV010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707440
            Sequence Read Archive: ERR13197169
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288022.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288022.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:09:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,052
            CDSs (total)                      :: 2,990
            Genes (coding)                    :: 2,898
            CDSs (with protein)               :: 2,898
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 25 of 92
            Pseudo Genes (multiple problems)  :: 17 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707440-rid22576513.guided.287"
                     /isolate="VRE5013"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..837
                     /gene="aac(6')"
                     /locus_tag="ACG8FK_003052"
     CDS             289..837
                     /gene="aac(6')"
                     /locus_tag="ACG8FK_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5098544.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          323 a          129 c          187 g          263 t
ORIGIN      
        1 aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca
       61 aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct
      121 gatctttcct attttgaata aagtgtttct gtataatttt tttattttat aagtaagata
      181 aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg
      241 ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt
      301 gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact
      361 tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa
      421 cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct
      481 caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag
      541 aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga
      601 atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac
      661 ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga acatccgtat
      721 gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg
      781 gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa
      841 aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac
      901 tt
//
